LOCUS       DAETQN010000001       108100 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.001, whole genome shotgun sequence.
ACCESSION   DAETQN010000001 DAETQN010000000
VERSION     DAETQN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 108100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 108100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..108100
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.001"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(32..562)
                     /locus_tag="K0W68_000001"
     CDS             complement(32..562)
                     /locus_tag="K0W68_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HBI3071697.1"
                     /translation="MSEINYQALREVAERAIPAMERLLMLPADDDLLSEQELKDSGVD
                     IDALNAFKFLAGPETVLALLDEINALEETRINDVCRIAELTKQLELAKSKLNEQREYY
                     EGVISDGSKRIAELESDSQAQKLVEAIIVAIENEQERLFDEDYLMDSKECIDVIREEV
                     KRWNDSRAAGIRIKGE"
     gene            complement(549..803)
                     /locus_tag="K0W68_000002"
     CDS             complement(549..803)
                     /locus_tag="K0W68_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071698.1"
                     /translation="MSNSARLQLGFSPLSKTIMLAKMRDVEGGRMRVGNDPGRDVTNE
                     AAQLVWQLVMAEGGEIVWELDDGSRMVLKAEKQEAASERN"
     gene            complement(800..1342)
                     /locus_tag="K0W68_000003"
     CDS             complement(800..1342)
                     /locus_tag="K0W68_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071699.1"
                     /translation="MSNKIENPVVLIHKRENHDSYEVAITNGSHDYYDGLLMASVSPD
                     EADNSFAVFAMVGYYMAAEIEKLRAQRDALAAENAALKESERAFDAMCAEEHGDNWVS
                     ELTETPATDAFLAEVRAQGVDAAIEAAKNLVAQEYEYKDFKAAQSDCCMHPGSDLVGK
                     VEMTEWLVDFAAQLRKGGNQ"
     gene            complement(1481..1861)
                     /locus_tag="K0W68_000004"
     CDS             complement(1481..1861)
                     /locus_tag="K0W68_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071700.1"
                     /translation="MFGIDAQRIAAFAKSPLDNPLSRSEQMELARLFLHIQKQADIFN
                     NMSNQPILDGHIQMVINSHEKGWAAIVPCTITYRLAKEVQEFRKASVESESTKAAINT
                     LIRMGFTWTGGAYWQAPHPILYGN"
     gene            complement(1861..2565)
                     /locus_tag="K0W68_000005"
     CDS             complement(1861..2565)
                     /locus_tag="K0W68_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071701.1"
                     /translation="MNNLQLEHFNVTGHSDFPFKFTLKGYAEDAVGQIIIDKGIVKFE
                     GDFDESAKTFIDFIAKRWSEQWKDLEKRANEFDRFMDTMDTAKEALAAGTPLDLESLF
                     NSTMASEMFATMFAGEFVRSGAKNYLELDYNVPAIGDFVVTIQREEGKTPGERVAELE
                     AVVDQRNGECDRLINELHALREERICAGSNTRNAADIYFQLVEECQIPPGGSLVDYVR
                     HLMAEVNSSHKDGEVR"
     gene            complement(2627..4312)
                     /locus_tag="K0W68_000006"
     CDS             complement(2627..4312)
                     /locus_tag="K0W68_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBI3071702.1"
                     /translation="MNTALSIMGDASSNTAIDYRQEMNVIHEIVAECEKEISFMYQVH
                     DFVYGDERHNMINRLLRLNHRPDEDRSRLNRVWLDNVDLEWVKQNIWAEYWRKVTDMT
                     NVLLIMPASRRDEWRELFIEGKQEVIKTDRTGYQMKVKEFVGVPEFKAETVIPTMLNL
                     LNDRHKYLSERVYGLFKALSPAHKTNKTNGFSERLIIADCISDFWRDSVSVNYRKEDY
                     IDDLRVLLHFFAHKEFITINRTAEVLSAAYRANDCQTGDWMNVDGNLMRVKMFKNGNV
                     HFEIHPDVAWKLNEVLAYSMPAAIPAPCRTAPKTRAPKQFGLIQKTISVPVRTALRDG
                     RLSKDKGVWYFSDSALQKSQVEELERTLSFIGGVQEKKHWQFPYEIGHTLNTIVATGL
                     IPDTKSHQFYPTPRLIAEYVARATELKPGEKLLEPQAGRGDLLAYINADLEDVTCIEI
                     APLFADILRGKGYTNTICCDFIKWSEDNAGYQFDKIVMNPPYSLGRHREHTLAALGHL
                     KVGGRLVAVLPGTAPILDWMTMDNYVYARGKSFTNEFEDTGITVSVYVFKRVK"
     gene            complement(4416..5030)
                     /locus_tag="K0W68_000007"
     CDS             complement(4416..5030)
                     /locus_tag="K0W68_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071703.1"
                     /translation="MFGMSITQSNGNVWMSPEFTPQNLINRGTMSTSKGSVFKTSIPS
                     NKSCFFFIKSSNKANMMFVHEFRDGYNVLRLNQVGGNPGTITVYAFSDMVLPHSGYGI
                     AMYNSAGKMVYHGEMMPLDAEIITITNPQFTINMGYPCAIMPAMVGVYNYRRTPYDRP
                     VYVTMTGATGNQIYNGEWYSSNVTWNVKKLYTNKVLVINTSKYD"
     gene            complement(5372..5941)
                     /locus_tag="K0W68_000008"
     CDS             complement(5372..5941)
                     /locus_tag="K0W68_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071704.1"
                     /translation="MCEKCKAIADEQNALFEEMDANELVKMLAILRGIEDVSIFERVV
                     TALNFESTFEEPTQVVALAHHFGVHYLAEKQRADKLQETLDMVIETQGTHDSNKSETI
                     IASKDREIAGLKSSLTMLMSAFNLMSSQAGYKMPSLNSDDPMVVRQLLGAMADQLDDT
                     KSRLEDMMRELSHRHNLATQPHKAFQSSN"
     gene            complement(6081..6239)
                     /locus_tag="K0W68_000009"
     CDS             complement(6081..6239)
                     /locus_tag="K0W68_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HBI3071705.1"
                     /translation="MLERFRLVTVIALLVMAVLVDFTGKMMSVISDGVLIGLAIYFAY
                     PLVRKATC"
     gene            complement(6239..6664)
                     /locus_tag="K0W68_000010"
     CDS             complement(6239..6664)
                     /locus_tag="K0W68_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite/colicin resistance protein"
                     /protein_id="HBI3071706.1"
                     /translation="MLGFFKKKTRKAVIEVKKMENRDAVEATVWGGYMISYANGTCDA
                     KEISILEKTIAALPAFSPFAGEIAQMSANIRAQYEASPRRANAQALRELADVAGTDDA
                     VDVLCLCLDIADQDGIDEPEEQALKKIAQALQLSLDAYL"
     gene            complement(6758..6946)
                     /locus_tag="K0W68_000011"
     CDS             complement(6758..6946)
                     /locus_tag="K0W68_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071707.1"
                     /translation="MNWFSNHFGKIWLAILALMAAGWVSNIIKLVCSGDLQFQAGMTL
                     ARVVGIFIFPVGSVLGYF"
     gene            complement(6956..7450)
                     /locus_tag="K0W68_000012"
     CDS             complement(6956..7450)
                     /locus_tag="K0W68_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071708.1"
                     /translation="MARQTYFTSVTKRPRSLRQILAELFSGRVMSRLDELETTVRLLN
                     ERLDNQASVVANVWAIVASGSSREAKNARPLVKEKNNKDSSNGKFSKKEAETNGLRSH
                     YSFTGDGSRSSRSEPFDAGFIHHHTSVDDNYHHSSRATCHSGWDDGGCDTSSSSSYSG
                     SCCD"
     gene            complement(7596..8189)
                     /gene="rnhA"
                     /locus_tag="K0W68_000013"
     CDS             complement(7596..8189)
                     /gene="rnhA"
                     /locus_tag="K0W68_000013"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBI3071709.1"
                     /translation="MTTKTPARPQAKTRKKDKNKNSPRTNPTTPVVEFNPQLKTVKIF
                     SDGSCLKNPGGPGGYGIVLQYRGEEREFSDGFHSTTNNRMEMMGALIGLERLKYPCNV
                     ILHSDSQYLKNGMTQWMKWWKRNGWMTSDKKPVKNVDLWKRLDEAASRHNVRWKWVKG
                     HAGHRENEICDRLAKIAAFSAADMPHKKDIGFVYNKQ"
     gene            complement(8771..9001)
                     /locus_tag="K0W68_000014"
     CDS             complement(8771..9001)
                     /locus_tag="K0W68_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071710.1"
                     /translation="MSYPTNVVALVDSDFLAQAREMMKDREQAFNLYEWAIKCLHLGE
                     HRELVEQLLGELINEVFALNVQLHGRENNQSK"
     gene            complement(9189..9782)
                     /locus_tag="K0W68_000015"
     CDS             complement(9189..9782)
                     /locus_tag="K0W68_000015"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HBI3071711.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPNFIGIERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATEYANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKIPLDVMKSLGGYDPANVIRKKRPSKKAA"
     gene            complement(9965..10774)
                     /locus_tag="K0W68_000016"
     CDS             complement(9965..10774)
                     /locus_tag="K0W68_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071712.1"
                     /translation="MSSVENVMTNDDLDELTAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GLDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVINAESGEAAENEPEQL
                     GIIEVEDCVEVNDELKVQQSNDSNTNKKTRAARGPSFTLSDKDDSFFNKAGLEKDIFL
                     DAYENAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            complement(10935..11492)
                     /locus_tag="K0W68_000017"
     CDS             complement(10935..11492)
                     /locus_tag="K0W68_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBI3071713.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMEACSAWVAHNGEAFDIPF
                     IRHEFSGYGVRLPDVPVIDTMLSGLWATEDGKRPRLEELAFSLGFIYDHAKAHSALYD
                     TNLMMQCFFKARNKYGFFKLPSEIV"
     gene            complement(11502..11921)
                     /locus_tag="K0W68_000018"
     CDS             complement(11502..11921)
                     /locus_tag="K0W68_000018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071714.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGAQVGEAINIAMSQFSEDSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TASGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            complement(11983..12627)
                     /locus_tag="K0W68_000019"
     CDS             complement(11983..12627)
                     /locus_tag="K0W68_000019"
                     /inference="COORDINATES: protein motif:HMM:NF024911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI3071715.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGESMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            complement(12627..13103)
                     /locus_tag="K0W68_000020"
     CDS             complement(12627..13103)
                     /locus_tag="K0W68_000020"
                     /inference="COORDINATES: protein motif:HMM:NF012413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBI3071716.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETVFTQRASEEEPGFSQIVYRRS"
     gene            complement(13100..13501)
                     /locus_tag="K0W68_000021"
     CDS             complement(13100..13501)
                     /locus_tag="K0W68_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071717.1"
                     /translation="MYVLCEIEGVLANTSHRKSVPDTDAGQLIAGDELIFPTSRMLRG
                     FARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNGTSYEKHIKRTLAEHKG
                     DLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            complement(13515..14618)
                     /locus_tag="K0W68_000022"
     CDS             complement(13515..14618)
                     /locus_tag="K0W68_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBI3071718.1"
                     /translation="MSLLLNREHTNGQVTNASYAKVIETVLKSGVQTDDRTGTGTLST
                     CYVPSYYMLTGGTVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWADE
                     NGDLGPVYGKQWRRWEDTRIVSHSEYLSKIATFRERGYKVEGYLGISEDRVVLSREID
                     QLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMATD
                     IGLQHSRFGYESIYTKMLYDLEMDGSVTETELDELGIPKRILNSCLVQRSVDTFVGMP
                     FNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMKRQAPEHSDPVVI
                     FPHEWSELDDFKWDDILILGYDPLPWIKVPVAV"
     gene            complement(14786..15655)
                     /locus_tag="K0W68_000023"
     CDS             complement(14786..15655)
                     /locus_tag="K0W68_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphohydrolase"
                     /protein_id="HBI3071719.1"
                     /translation="MNFTKLTDHLKLATDRLIGFKPEPYELHEGHGVATESIYKMVDQ
                     FHELFQHPRRVMPTPELLRLRASLIHEEAVVEGIPAAMNGDIEQLLDAMADFLYVGVG
                     TMVAIKGGISTGMTYYTQEQSIDRFMQTIFVPGNTVFDDMAMPFQEAREASCMLEELA
                     DKLENKTVKDSELIQELRRVMNKIYVACMMTYRLADFLGINVVELVGEIHRSNMTKLW
                     PADVEERRQAVANCKYDSSDLGFRHADGTDKMIGFRISDGKILKSPTYSDVDLSSFVE
                     QAKASAMYGMIKK"
     gene            complement(15733..16875)
                     /gene="nrdB"
                     /locus_tag="K0W68_000024"
     CDS             complement(15733..16875)
                     /gene="nrdB"
                     /locus_tag="K0W68_000024"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBI3071720.1"
                     /translation="MSYSTFRLGANDATKEPMFLGQSVNVARYDQQKYRDFEKLIERQ
                     LSFFWRPEEVDISSDRIDFNTKLRDHERHIFLSNLRYQTLLDSVQGRSPNATLLPLIS
                     IPELETWVETWSFSETIHSRSYTHIIRGMVDDPSIVFDGIVTDEEIINRAISISAEYD
                     RLYGMTCERQSLGEKEFERLYVNEYGWEPYPLHRQLFRTLVSINALEAIRFYVSFACT
                     FAFGERKLLEGNTKIMRFIARDEALHCEGTERMIRFMRTGREGLLWKEIAADEENVIY
                     DTMKSVAEQEMNWAEYLFKDGSMIGLNADILKTYVKYRTNLAMNRLGLKALFPEVTTD
                     PLVWMNKWLLTDTLQIAPQEAEQSTYLVGQIDSTVDKASLSQFADL"
     gene            complement(16981..19296)
                     /gene="nrdA"
                     /locus_tag="K0W68_000025"
     CDS             complement(16981..19296)
                     /gene="nrdA"
                     /locus_tag="K0W68_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBI3071721.1"
                     /translation="MISIVKRNGQTEPLSEEKYNRVVMYGVEGIRGVSASAVAMGAAA
                     SIFDGMTTSQLHEALVKSAADLISPEAPNYSQVAARLNIFKIRKDAFGRYDYPNFYQH
                     IVKNVNKGVYDKDLLTHYSFEEIEELGNYIKPKRDDLFGYAATVQLQSKYLVQNRVTG
                     EIHEGPQHIYMLVGMCLFQNWEDDCAGKTRMEMVKGFYDVTSTFKLSLPTPIMAGVRT
                     PTRQFSSCVLIESGDSLKGISAASAAIIDYVSRRAGIGIGFGRIRALGSEIRNGEATH
                     TGVIPFLKHFQTAVKSCSQGGVRGGAATAFYPIWHLEVESLLVVKNNRGIDENRVRHL
                     DYGVMSNRLMYRRLVRSENITLFSPHDVPDMYEAFFTDQELFEKLYHKYEADDSIRKK
                     SVPAIELFSSLMQERASTGRIYIANVDHINEHGAFIPALAPVRQSNLCMEITLPTRPL
                     AFTDDPNGEIALCTLSAFNLGAIRSLESLKEVAFYAVAALDSLLDYQDYPMEAAEVPA
                     KARRSLGIGVTNFAYYLAKNGIRYSDTAGNKLVHETFEAIQYYLLDASCQLAEAKGEC
                     DWFEQTKYAIGQLPIDHYRSSLDESGETNFELKMPWEELRERIAKYGLRNSTLTAQMP
                     CETSSQITNSTNGIEPPRGPVSVKSSKDGIVKMVVPEIEKLKEQYEYLWDMPDNRGYL
                     TKVAIIQKFFDQAISANTNYDPSRFEGDKVPMMTLLSDLLLAYKMGVKTLYYHNTRDG
                     AGKRDDDEPQNPLTQAVAVEPEDECDGACKI"
     gene            complement(19370..19939)
                     /locus_tag="K0W68_000026"
     CDS             complement(19370..19939)
                     /locus_tag="K0W68_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071722.1"
                     /translation="MSKKISVVGVDPSMSNFGLAVGTLDLETDELEIHDLTLVETKAG
                     SNKKTVRVNSDDLRRASEIWRVAKPIIDKANMVFCELPVGSQNSRSQTSYGICIGVLA
                     CVDKPLIQVTPNEIKHFVGNKLTTSKEEIIQWATKKHSKAPWLRRKQSGQDVLVNKNE
                     HLADAVAAIHTGMQTDQFRQVRDVLKSLI"
     gene            complement(19949..20692)
                     /locus_tag="K0W68_000027"
     CDS             complement(19949..20692)
                     /locus_tag="K0W68_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071723.1"
                     /translation="MSFKLSRSQFLQVFAVMQSIKLINGHTSNGAAPRILWGSNNIDG
                     VQFAALLGLISETPLMQSLKSLPPGCIAPILINPFVEGGYLPNVGPGFIASHENEDLN
                     INSEGFFGGMGAHHCMAFTNLIRLANKRVDSLASPGDAFTGFLIQRRDKKYSADKLQF
                     VGKYGEMVEIELQLPHVLANDSADSRRLLGIMRHFIASGVKHAVDKRVTQENEYSDFA
                     NSPQPTLQTAIVTNSLEARLLENPIWGTW"
     gene            complement(20682..22598)
                     /locus_tag="K0W68_000028"
     CDS             complement(20682..22598)
                     /locus_tag="K0W68_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI3071724.1"
                     /translation="MKFLKLQVENFMAIASAEVELDQRGLVLIQGVNSDDSSASSNGS
                     GKSTLMNSLMWCLYGETAHGVKGDDVLSTDHEKNCRVAVTIEDEGKRYAIIRHRKHKE
                     FKNRLIVRGEDGDMTKGKDALTQEFVERLIGASKEVFMASIYASQEAMPDLPGMSDKN
                     LKTIVEEAAGVDRLTRAYAIARERANAAAARMDVVKTKLESTISTIEATQSEIESAKA
                     SSESWEQERSKRYDDALAGLASAEVELTEVELEIRTLPEQIRDTEKAIESERKKLASK
                     EEHDAKLLKVRGAITDIRASIKATENSQADAMNRARNFKTKAEEVGTKVGSPCPTCGK
                     AYCEEDLSTVKENFIEQARQEIGQAKTLAEAMAKHKTNLEKALSIESALVKTTPDVTA
                     IIARIEELTKQLSSLRHREKEIVAIESLVTRARTEVDRISKEINPFIALIARHEDNLV
                     SSKSTFKSLKDELKAIQEQTLLLEKARQVYSPAGVRSHILTSVTPFLNTRTAEYLNTL
                     SDGNITAEWSTMDVTKKGEYRDKFNISVQKKGSSKSFQTLSGGEKRKVRIACSLALQD
                     LVSNRASKNIDLFIGDEIDDALDTAGLERLMGILESKARERGTVLIISHKEMKSWFRE
                     TITLEVKEGRSYVV"
     gene            complement(22828..23913)
                     /locus_tag="K0W68_000029"
     CDS             complement(22828..23913)
                     /locus_tag="K0W68_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBI3071725.1"
                     /translation="MTLPYGVISDCHYHKWDAFSTTNAEGLNSRLEIQLEATKEAAIA
                     MKKAGCKYMLVAGDTFHVRGTVSPSVLHYVTETYKWIINELDLTVVMLAGNHDLETND
                     SVYSANAAASLSSIGVVIVCGKRPHSIKIGDVTVHLISWRNNHAELISDLKALRKSVE
                     GDNHDVVIHTSINKAIPTMPDVGIDAQELKDIGFRLVLSGHYHNHKEVIPGVISVGAL
                     THQNWGDVGSLAGYMIVNPDGSFSHYETSAPKFINLEDDVADDQIRGNYVRFRAVIEN
                     DEEGIKYQNILKTMGAKGVVCNFIRKSSMMEGTASTTETSKIDSLGESVSAYCKIVHD
                     TDGGFDLSKLDILCQEILTEAESSEAV"
     gene            complement(24168..24812)
                     /locus_tag="K0W68_000030"
     CDS             complement(24168..24812)
                     /locus_tag="K0W68_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071726.1"
                     /translation="MDLKRTRWVRRLEDGSYTIESNSNLNKQKLLCDICGIAAKCPIY
                     ETRIKLDKAGVNFHLNSCIRYVPLLAFRKPIIGLDAPYFNTLRSGVTWRDRLSPDKLI
                     CLVSADTGKIIRFGKVDKVYSGPVDEMLRKHSRFNHLCMGGEKIEKVEEVIRKSYGHF
                     LTKDSLLTAIYIRHVKREFDLEYHSEEELNLVDPRPKAGVISINAARKKPTDAL"
     gene            25014..26228
                     /locus_tag="K0W68_000031"
     CDS             25014..26228
                     /locus_tag="K0W68_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBI3071727.1"
                     /translation="MLSAVIDSLSAVHRMLPTVFGILSAANQVCCQRLNDKGVHMSTK
                     NKKGKNNKEVEDNIDNFEEGSLELYTGELVPNSNNTVQPIALMRLGLFVPTLKGTKYS
                     KRNKPNEIDASKELVQLEVARSEGYSDIKITGPRLDMDHDFKTWVGVVRSLAEYGEPN
                     GRVELSITKFAKFCGYPSSQIRKTLRDRLTNSLLKIMRTTLSFQRTYEEKNVDGSNKI
                     SLLMVHLINSVDYNEQKDTVVFYAEPKLAELYRFDHKVLLQLKVINKLPRKETAQALY
                     TFIESLPTKPAPVSLARLRARLNLSSRNVSSQNQTIRNGLKALQDLGYLEYSEIKRGR
                     SIYIQIHSRNPKLKVAPPKPEDIEPKKPDEKAGEIDAKQNIINKITELSQNLTPENIK
                     MIEILSNSLKLL"
     gene            complement(26808..27020)
                     /locus_tag="K0W68_000032"
     CDS             complement(26808..27020)
                     /locus_tag="K0W68_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071728.1"
                     /translation="MALTLAGLEIEKTSGYWRAKGFKQPGILERLEREDGYIVHQRRE
                     WRMYDPETGKLTTKAGTLWGLLKKIH"
     gene            complement(27020..27355)
                     /locus_tag="K0W68_000033"
     CDS             complement(27020..27355)
                     /locus_tag="K0W68_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071729.1"
                     /translation="MKTIPFALLFLSSIVVADTTVYQCEMSVADVKNGALTDVIKAPY
                     GAMVVDSGDQFYVVRDDRVLSSPYLTNRNGKLTGVGEDHFVYNKYKGFYGVHASQQSY
                     LFDDCKEVG"
     gene            complement(27571..27846)
                     /locus_tag="K0W68_000034"
     CDS             complement(27571..27846)
                     /locus_tag="K0W68_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071730.1"
                     /translation="MRVLVRIVTSTVYDVFPLFMVKVDGLNDEETDALIQRTLVEYTG
                     HDADSVMVDDDGVCWHNGNCWYVEETQQISNEDAEHLERILSISTFE"
     gene            complement(27902..28327)
                     /locus_tag="K0W68_000035"
     CDS             complement(27902..28327)
                     /locus_tag="K0W68_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071731.1"
                     /translation="MAAIKKLYDAANVALDVIDDEVAKGFPEPDWAHQLRNAIAEMTP
                     PDPTPDETDWQRFIRMYAQEIGPTPTAEQAMLLKYFKEAGEDLPIDDSAYWFHCAWRK
                     YDVIFTQGMGSKDMVVWHLLHIDTAVDRVIEQFFPNQED"
     gene            complement(28389..28778)
                     /locus_tag="K0W68_000036"
     CDS             complement(28389..28778)
                     /locus_tag="K0W68_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071732.1"
                     /translation="MLLIQPGFGLKIKKGHMFGERDSKRKILSIRLPFITIHWLNRES
                     TDYWYKCARAAFNDPDWFVENHHAVRQAKRKANTTYMKAYQDAWEEHRGRYQKNIEKL
                     ESENMDLRRKLGEARRDIEAYKRLVSE"
     gene            complement(28799..29539)
                     /locus_tag="K0W68_000037"
     CDS             complement(28799..29539)
                     /locus_tag="K0W68_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071733.1"
                     /translation="MKRKIEEPKTTLNKHGEKIQTHPAFGLVKTSRVSTTGIRLFDSE
                     LEHRDYIEIGVYEAELSLDNERTRPQRSCNRRPLVEIRLSQAQWAAMVSSFGVGEGVP
                     CTISYKTIGDSIRVHDIAEQKSIRQKFNDQIKAATNSEIDRLNQQVSELGELVKKGRA
                     GKRDLEELYRKLASAVANLPENLCFATELMQEQMDEIVSSGKAEIEAYVAGTVMRKGL
                     AALGTESCINDDPTVSITKMESGKELLP"
     gene            complement(29587..30411)
                     /locus_tag="K0W68_000038"
     CDS             complement(29587..30411)
                     /locus_tag="K0W68_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071734.1"
                     /translation="MPNHITNEIRVIGGTNKQRLDFIRAITNKKGLIDFNTICRRPKS
                     IEMEENNHIRRMASAMAGETVYDHMFGELKTPEQVELAMRESGMTRKEIRKIKEQALM
                     RLENYRRYGYYSWFDWSRANWGTKWNAYNIEMPVKRIPTRIKYGHNFRHTHVRAYAKR
                     IYKKRLARHAATGGELVIRFETAWSMPKPIYQAMARKFPHLEFVIRYADEDFGNNCGF
                     ILLRNGKWEYDYIAPANNKQSSEEKIKWRKFAFELCCPGITPQEYGLNEEYEYAGY"
     gene            complement(30507..31337)
                     /locus_tag="K0W68_000039"
     CDS             complement(30507..31337)
                     /locus_tag="K0W68_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBI3071735.1"
                     /translation="MKKGLLAVALAAICTMGLTGCDRVEPGYVGIKVNKLGEDKGIGE
                     VVGVGRQWTGLNTELYVFPTFKQMKTYDEPFTFQMSDGTAIGHKIGVAYLVNRDKVTT
                     VFQTYRKGVDDITESDLRQKIADSLNRLASRMTTDSFIDGGKAQLLDNALKDIQKEMS
                     PVGIEVLSLSWVGKPDYPKTVIESINAKVTANQRTLQRQQEVEQRKAEANMLREQANG
                     EADAIRARAQAEADAIRLRGEALRQNPNVMELEAINKWNGQLPQYMTQGANTPFITVK
                     "
     gene            complement(31341..31541)
                     /locus_tag="K0W68_000040"
     CDS             complement(31341..31541)
                     /locus_tag="K0W68_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071736.1"
                     /translation="MIKIYLLAVATGVSVALIYGLLVPSLISTKSDLAVMFGVIVGFG
                     APVIGLIAGRKFINSLIKAKGK"
     gene            complement(31634..32515)
                     /locus_tag="K0W68_000041"
                     /pseudo
     CDS             complement(31634..32515)
                     /locus_tag="K0W68_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
     gene            complement(32710..32976)
                     /locus_tag="K0W68_000042"
     CDS             complement(32710..32976)
                     /locus_tag="K0W68_000042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071737.1"
                     /translation="MLKSIINGATTTPTQLAKEIVFYHGEYAVIALPSILGAAGMKAT
                     DREFGLVSEQVVKILARVSRLLNHDAIVFDESAALKRINETKGA"
     gene            complement(32976..33920)
                     /locus_tag="K0W68_000043"
     CDS             complement(32976..33920)
                     /locus_tag="K0W68_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBI3071738.1"
                     /translation="MNYLFVDGNSLGYYHQQSDKLHNGEMEVQAAFGFVKNVRRYASI
                     LHARPMILWDGFSDKRRDFYPDYKANRDDDPDMKKMKEGFAIQKPYILKMMTALGVTQ
                     LIAKDAEADDLAGLLVASIAPQPIVEHIYLLTGDSDWLQLVRENVSWVSLREDAKNKQ
                     VNFEQFAELTGFATPRAFLEAKALQGDKSDNISGVGGIGAGGAKELLHEWGSVATMVR
                     GINDGSIVVDKGRHKTAFNKLAKNAFNEKTGCRMLEAFKRNITLMNLIETKFPPTEIE
                     TIKGNRDVKAFEQLCYELNFRSFLEDLEVFVLPFERYC"
     gene            complement(33981..35009)
                     /locus_tag="K0W68_000044"
     CDS             complement(33981..35009)
                     /locus_tag="K0W68_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBI3071739.1"
                     /translation="MSKTLLDLLNKTREDIAAKRGNNVDLTRLKDGVNYIRIFPNKDD
                     PNGKFFQTFGMHYVKYQNEEGKEATNAYICEQHTHGRACQLCEMVMEGRARHKGNKAM
                     EERIGQMRATPRYLVNGILSAREDFADAEKCQLIELPSTVFDDICKAITEDIADDIGN
                     PLSKEEGYAFLIKRTGSGRDTKYDVSPKRKVYKGDIEDKFWNTQHDLIAYANQADETR
                     LLSTVRTMGRLIGIAAPTAAASAPAISSTAKTSAAALPGFGSVTGHTEGATAVATAHT
                     PASEPTSLVDEEILRAVETEFKPEASSAAVAVSVKESEAVAATSVAAASATEDEGLDD
                     LLRELDSL"
     gene            complement(35127..35558)
                     /locus_tag="K0W68_000045"
     CDS             complement(35127..35558)
                     /locus_tag="K0W68_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071740.1"
                     /translation="MITDIYEKIMSDLEFDRDNLEEVWRRQPRLLMEYGSKLAHAERS
                     VAEAKLNLEAVEAKLYDTERKNLSMNGIKFNESVLDAKVKTNPQYLSKRQKLDDARHI
                     ADIYKHAVAAFSHRRDMIVQASKMAIVELERLGSERFITPR"
     gene            complement(35684..39202)
                     /gene="dnaE"
                     /locus_tag="K0W68_000046"
     CDS             complement(35684..39202)
                     /gene="dnaE"
                     /locus_tag="K0W68_000046"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBI3071741.1"
                     /translation="MKALMVRTDFSLGESALKAENAVKIAREAGYTAVISADSMNIAS
                     VIPLQRAAGDDMAVICGVKLNIVDDPTYEHRDKLAKESKGCMESLERGRNYSFTALIK
                     NEQGYRDICELMTVANTREQFYFVPRLSLEQLVSTYAKGNIILLTSDIGSVFQRNDFA
                     KIISTLITPGGKDNFYSVVYPHPTPFYDQINVRAMKVASALKIEPVAFYPAYYESIDD
                     ADIKDIAHMVTNNIKIDQPHRLRIPHQRDNAVNGRRHLLEALKAFSVRMDVPVTAAMA
                     STTQDTIIDACTWRWHELPPALPKMADDEPATLMKLAVAGLRKRLTTKEFGYTPPASE
                     NRVYVERLKYEMDTLTRLGFCGYFLMVRDLMNHSRETGIPVGPGRGSSAGSLVAWCIG
                     ITNVDPIRHGLLFERFINPERLDLPDADLDFSQARRHEVIEYLNERYGEDYVAGIPNF
                     TYLGAASALRDTARIYGVESADMAVSKELKNVEDDSLPLEELREQLASLDKYATKYPD
                     AFNAACKLQSLMRGFGRHAAGMIVAGVPLTERTPVERRGDARCIAFDKRYCEAMGLIK
                     LDVLGLATLDLLDSAKRYIKENTGEDINLDTISLEDRKVLDGFAAGYTQGVFQLESGP
                     MRKLLKDLGGGIEPMSFKTVVATTALFRPGPIQSGMLDDYVSVAKGFMTPESLHPVLD
                     ELTAETNGVILYQEQTMNATRLLAGFTMAEADGVRKAIGKKDMEKMKSMGEKFIVQAQ
                     AGWIDVELEDGTTQRIHRAEHFKCEDGTMKTVEEALEYGAKLPINAVRVTASHPGLSE
                     MKAKEIWTAFEKNGAYQFNKSHSVAYSLISYQSMWLKTHYPAEFFAAALTILGEDKHQ
                     GLVKDALTYGIRVLPPDVNVSSNRIEIRTLEDGSQVLYAPFSAVKGCSENGCQAIMRA
                     REKVGGKFESLAQFEEAVEKRACNSRVRESLQKVGAFASIEPGSLPATDPERLRDQAE
                     LMGNLVIDAVKASRPFEMNSKRSAEINVLMTRMAAEMGLGDELIRPSIGIKPKIMIIL
                     DNANGNDARTGYFMENGYDDFKAKLLTAGDLRMGDLYVTGVCKKVKDKEKDYTKDEIG
                     QFTDFMREEINLVRPTYVLTCGSRATALFNNKNKPSDLVGRKEYLPDLDVTVFYGFNP
                     NILYFRPEEGERLEAILADVAETIKS"
     gene            complement(39383..40618)
                     /locus_tag="K0W68_000047"
     CDS             complement(39383..40618)
                     /locus_tag="K0W68_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI3071742.1"
                     /translation="MTTTALQNEKNPSDYLVCKWCGKSFHYFKSHVANGNCEGIPESV
                     KDADPDTVLKMYTTQFPDEPTLSKKALDAIQAKRAEQKSEMAKSSGLTSSPGYTGTVE
                     YKTDLVAAHELLNVTVEELGTKRGTPLMVSVNVNTPYPEFVPEVKKGYVYGDFELIKD
                     IFMMLELGIPGYLWGHAGTGKSSLPTQLCALLNRPLIRAQHTASMEEAHVTGQILARD
                     GSTYFEPGLLALAMKHGWVYLADEYDFAFPQILGVYQPVLEGEALVIKEATPEWRRIT
                     PHERFAFIGTGNTNGSGDETGLYQGTNIQNAANFSRFGIVSNVKYMSKDAEINMLTNA
                     GIVDEYAEKMVKFAGIVRDGYEEHNISQPIGPRELLLSAKIGMMRGDFVTGIERSFIN
                     KLPSASAQAAREVVQKIFG"
     gene            complement(40714..42822)
                     /locus_tag="K0W68_000048"
     CDS             complement(40714..42822)
                     /locus_tag="K0W68_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobT"
                     /protein_id="HBI3071743.1"
                     /translation="MSKKRSIKEVQDFRDSVKRVVALLSGKNIPVAERGDDAYVRYND
                     DGEPILVNIPSIPDNATPALMNAVRGFLDHEVAHILFTDIRVSNKMREKGRVPSRSLG
                     NALEDVFIERKMGQVFNGTRRNLMATQRLIIEKVFKPKASEAIAYCGKDQRALFLNFF
                     LCPVVRAWDGQAPFVDFMDEYWPVIEKPISLLKEHGIDVAVRNMSCTEDCVKVAATIA
                     KILKDTESESKGKESAPGKTSDPSDADQTDASGENNEDNEDHETPSALDNHKSIESES
                     HSKHKHDNNDSDDSDNSESSETIFDDTENDKEVSDSDASDNAASESLTADHEKRKTTE
                     DGSSDIPTPSKMSLEEALEELDSIEDEVGGMTEDALSETIKSELTESSKSEYRPYNRS
                     YDFIGSIDQAEAHIKRLIKTFSDIDLGGYPISRYRIVPEGNQLFDKYIEKHLSSGVSS
                     TLAKDLERAIASRNRVQFIPGQRRGRIHGSSIYRLAMNDDRVFRKKEESKAVNACVQQ
                     VIDLSGSMSGITIQLALASSYTIADALDRINVPNIITGFTTFGSHMAAGELKAVKYEF
                     SRFESLMLPIIKNWNEKVSSREVRSRMGCVGYTFPLLNNVDGESIASLASLFSGRMED
                     RKIMLVLSDGAPWAVGRGFDAHLRSVAKQIETQTDIDLMAIGIMTDAPERFYSNHALV
                     TSVDSLGSSVVTELSRIILK"
     gene            complement(42921..43133)
                     /locus_tag="K0W68_000049"
     CDS             complement(42921..43133)
                     /locus_tag="K0W68_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071744.1"
                     /translation="MKTTISSLITLEIGHVEKLADECVADILTELPNEQIQVGMHDAT
                     AFIFLLNNKRFTLINTGSGSLAVRTC"
     gene            43381..43767
                     /locus_tag="K0W68_000050"
     CDS             43381..43767
                     /locus_tag="K0W68_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3071745.1"
                     /translation="MSDKNIEFIESRYAAFIAGLIESSPMSQAQIARLIGYKNANNLS
                     LIKSGKIPLPIDKVRPLALALGIEPSRLMMMVLEERQPELAAFLYKEGTAPLNEDEKQ
                     VLAAYNERFGKEKGASQKVVEAIKSL"
     gene            complement(43762..44865)
                     /locus_tag="K0W68_000051"
     CDS             complement(43762..44865)
                     /locus_tag="K0W68_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBI3071746.1"
                     /translation="MKNSFDRARAAENTSKEAIEYLERASQMQAIMISQINNNMIFSD
                     AFMLFTRLSLLITRRRPEIAVHCILIHVLPHIADVKVSDINRFMVNQLVNPLILDGKI
                     VMGRRVFSLMKQFLSWCAFQGMIDVSPLNDMSLNKVAGGAKPTPRERKLTDAEVWVFW
                     NIWDYFNVCAGTKWAARLCLVSARRPDEVLRAKKSEFNLKRGVWNQGKRNKSAREHSL
                     PLSTLMRTCIEELFEYGKDSQWLVPSNKKIGKDLPMSKVAIAQALRRILERPELMELA
                     PFTPRDLRRTARSYFPALGISQEVSRKIMNHSLEGIDRVYDRHDYMDEMRDALESFST
                     YIASIVEQPDLDEIDHKFKGDRLSTELIRLNFS"
     gene            complement(45073..46986)
                     /locus_tag="K0W68_000052"
     CDS             complement(45073..46986)
                     /locus_tag="K0W68_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBI3071747.1"
                     /translation="MSFIDYDILSASSSKELREKLAEKIAEGWQPFESPNIVEPSKGG
                     PNAYNQAIVRSGDLSQLPTPVSNEDEEPDYYYVVTIAGQSNSMSYGEGLPMPKTFDRP
                     HPRVKQLARRSNIKPGGFACKYNEIIPADHCLHDVQDMTKFKHPKANDSEYGCVGQGL
                     HIGKKLLPFIPANAGILLVPCGRGGSGFTVTGSEGQYYEESGAIESSLKWGLNTPLYK
                     DIVARTKAALSKNKKNVFLGVVWMQGEADLATGKYSQHNELFLKMVEGFRKELSQYDA
                     QCVFGSAANVPWICGDTTHWWKNKYPKQYEIVYGGYKGKTSQAIYFVPFMKDENDNNV
                     LTNSPTDDPDITSIGYYGSGHRKDNKTWTSESRESHFGSWQRRGIISDRLATALLAHS
                     AKSSAFVNGVTGEIIKQEQPTQPKNDNIVTTPTPRTVISLTGAGGDVATEQGWTVTGA
                     NIQSVENGKAIALTKAVSNETWTISKATDIAQALLENGGKLLCKFKLSGNPDPKKAKF
                     GFGIYYKTSKPVADDISFDGTGSLFLANFFLQTIGGKLNIMMHRTGNNRNVQIDSYGD
                     YDNNWHTLELQYEKGTGTVIPVLDGKAGSKFKLVKDNIALGDEAFVLTDITNNQTHDL
                     AIETLTISINNPT"
     gene            complement(47212..47298)
                     /locus_tag="K0W68_000053"
     tRNA            complement(47212..47298)
                     /locus_tag="K0W68_000053"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47264..47266),aa:Ser,seq:tga)
     gene            complement(47789..47864)
                     /locus_tag="K0W68_000054"
     tRNA            complement(47789..47864)
                     /locus_tag="K0W68_000054"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47829..47831),aa:Thr,seq:tgt)
     gene            complement(47869..47955)
                     /locus_tag="K0W68_000055"
     tRNA            complement(47869..47955)
                     /locus_tag="K0W68_000055"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47919..47921),aa:Tyr,seq:gta)
     gene            complement(47961..48035)
                     /locus_tag="K0W68_000056"
     tRNA            complement(47961..48035)
                     /locus_tag="K0W68_000056"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48001..48003),aa:Asn,seq:gtt)
     gene            complement(48795..49085)
                     /locus_tag="K0W68_000057"
     CDS             complement(48795..49085)
                     /locus_tag="K0W68_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type natriuretic protein"
                     /protein_id="HBI3071748.1"
                     /translation="MSNLTSWDWWLATYFLAVGVAFAFYVGQLVVKLLLIKFASHKRI
                     DDGLWRLGTLVETRYGQLKENETITIQAKRFTATITRTPSRRVALIKKVTTE"
     gene            complement(49443..50765)
                     /locus_tag="K0W68_000058"
     CDS             complement(49443..50765)
                     /locus_tag="K0W68_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HBI3071749.1"
                     /translation="MKKLIALKHKLDEMKAMGTNAKKEALANMDDFEQSMVSLMLNPF
                     IRFGVKKYKVAEPLSESIPSDEKAIDVLNKLASRELTGNAAIAAVESIVASMCADGQD
                     VFRRFLLKDPKAGVGISLCNKVFENPIPKFEVQLASPYKEKGDKYPFKPNPKAKWPMI
                     GSLKLDGLRVICEVIVDEEEVNFLSRTGNPITSLDHLKPAMLELGKLSGHKHIFFDGE
                     GTAGSFNQSVSALRKKNVQAIGAIYHVFDFFLPEWRAQAKSKEYAKTGMKLKERLAML
                     VALFKNDRSEGYTQDIHLHPFYIIHSHEDFIERFMKRLDDNEEGEMGKDPNSVYEFKR
                     TRSWWKLKDEDSEDGEIIDFEPGDPDSGFANTLGKIVIRLENGVIVRASGIKHKYLDE
                     IWNNKEKYRGRIVEVHCHEKTPDGSLRHPRLKWPRCLRDTEDRIGDKE"
     gene            complement(50762..51244)
                     /locus_tag="K0W68_000059"
     CDS             complement(50762..51244)
                     /locus_tag="K0W68_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071750.1"
                     /translation="MAVVVKADLSKIPSISGNNGYSLRCEEVKINGESAYCSYSVCQH
                     TILAFKENRLPRTSFQSCATAIKAGKCKALKMMVEEIRKGESLYFEDMTALIKEVEER
                     NKQARTLKRKRDSVTINSMVKKSTTSQTAITDVYAALLEETTKETHEQIDQHMEVKQQ
                     "
     gene            complement(51300..52076)
                     /locus_tag="K0W68_000060"
     CDS             complement(51300..52076)
                     /locus_tag="K0W68_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071751.1"
                     /translation="MKDEIQKLACDIIDKTGLEISESNRLDIIEKAVKTAMDHIATRL
                     VEIPLPGLPYLKVKLHVWGEPSCARRSALVVFISKENPLSLKVQVGAWLDGRVIYTNT
                     VFCLSNDETIEAAIQEAVLAMRSSGLMKNNYEEYLRSISGEKTLSLKADFVTPKNLLE
                     VLLNKGANDAVNVIRESEYASLCDMCKSQLDLVHIVIDAGKACDGVMAEFAWKVVRIA
                     NELPMIEQEAKSYATNHVTELLAPYRLESNQRKMISWGSW"
     gene            complement(52152..53267)
                     /locus_tag="K0W68_000061"
     CDS             complement(52152..53267)
                     /locus_tag="K0W68_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBI3071752.1"
                     /translation="MSELKELIAELDFEQWLDTEGIVYRRGGVSTRGREVNIKECPVC
                     GSSNWKVYFNLTSGVGKCFAGDHPEEIQFNKLVFLKHYSGKSRRQFEEYVQNALLSQG
                     WAPKKEELVLASTVELEGPVALPRHYELPIDGRLPDYLVERNISPEMAKYFDLRYCVE
                     GKHAYVDPYTDQVKGQIFDMRILIPVYDLDGVMKTFQGRDITGTAERRYLFPMQLPAS
                     GKFLYNGHNAVGKQTVVVCEGAFDVMGVKRAIFDEETLRDYVEPIGTFGMHLSGNTTQ
                     DAEDQLGAFLTLKARGLRNVIMMWDSEKQAIRNTMAAARRLTSIGLNVKVACLGEEGL
                     DPGGATPEQIIKAYYRAKPYTKQLELQSKVLGIKALS"
     gene            complement(53415..54632)
                     /locus_tag="K0W68_000062"
     CDS             complement(53415..54632)
                     /locus_tag="K0W68_000062"
                     /inference="COORDINATES: protein motif:HMM:NF015736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI3071753.1"
                     /translation="MPSQFANAANAILVNMVSGYYRMYKSAPSSSAILDMLKRAKRDK
                     TIREELFADVVAAFKRILAEKLSDTSYMVDQVSTFAKSVAFDDALIKAAELKEKGDFQ
                     GAMAIMAKVQQIGSNEATGIYDYYTSASERLKAREYEASEEYVPNSITTGLPLLDRLL
                     YQKGWAKREMVLFMGFAKSGKSTAMGEFSINATLAGYNVLYLSLEVHTTILSDRFDAR
                     LSETEMSKLVERRDEVHRKLAELGATKGIGSLWVVERPSGSMSPADLDRMLNSMKANG
                     MVPDMVVVDYADLMRASYDLRDDRANIRSIYTDLRALYDKHNVAGITASQTNREGGAS
                     EVATMMHAADNIEKVRIADLVITINKTEEEEAKGEARLYFAGSRNQQGGISIRVKQNL
                     EQMRFIERILDVT"
     gene            complement(54799..55539)
                     /locus_tag="K0W68_000063"
     CDS             complement(54799..55539)
                     /locus_tag="K0W68_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071754.1"
                     /translation="MSQIFFDTIDNDQYDFMTEWNTAVMDKWVAENIGLSRCKDEAEL
                     FETKWFDYRDMHPLMATCLFTEAYKRQYSNIMLTHGREHFETAPFTTGLKRLPYQELS
                     TANKTSLWKARQFADRYCCSYDYFISTVLSAAARRLWDKLPRPQHLWQPELVEIFEEK
                     LARRATTRLDDSLVSFKHMGDMQFNPIQESYFEWILECLRTIPRSKRIRAIFSAIWLM
                     EIVPERLISAHFPEELEEARRFIDPLSN"
     gene            55829..56887
                     /locus_tag="K0W68_000064"
     CDS             55829..56887
                     /locus_tag="K0W68_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071755.1"
                     /translation="MRMNRLPGYGLPELAFWPQPKYERNKWSMFCLKLRNDGTLAWYR
                     RYVDRGMPNHAVDDDYDNYPEARKAALELNKNVSFDIEKLPLSMFQKKSLRLKVDKAL
                     KAKSRLMDEEYIMLNEAIKAHANDPRVTLNELIINPDYETLRQPLFDVLSEMPYLHFV
                     FLPKFQVSLQLVAPNTWECSYNNKAKTAKICYQERIARGFGLSGAAHWGKTKATIRSM
                     LLPRANRLLQLASVKRMLDEALRNGQKVLVVGNFVFWFEDKNQVGWSVKVASESETTS
                     RGNTLWKEGTIISKNHGRIVVLPYTKENGKHVKGYTKNAPNDGNAIPRHKDEYVELPF
                     EILDGDLMFSLFGELNYE"
     gene            complement(56948..57301)
                     /locus_tag="K0W68_000065"
     CDS             complement(56948..57301)
                     /locus_tag="K0W68_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071756.1"
                     /translation="MKMGDLAKRKESDAPAKNTTPLRQPVRPQGRPTRGKEKIKSRTM
                     SLEDEYFELLEMMKFIPRFEKFTRSDVIRAAIFHLAEKSPQEIEDIVKLNEAITAADV
                     TMRTDEIKRELMKKG"
     gene            complement(57307..57975)
                     /locus_tag="K0W68_000066"
     CDS             complement(57307..57975)
                     /locus_tag="K0W68_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI3071757.1"
                     /translation="MTKIFVVGGTKGGPGKSTVAQQIAVCLKVKKKKKVYITDIDIQR
                     TTTSWCEDRRQNEDLELIPFAYVQDDIIKHLKSLQGRAEFVVVDAGGFDSEIQRQAML
                     MADVIIIPLRPKRRDLKSLRDIDPIIDNVRNVNDKVKVRAVMNQCPALPSQVSRILAA
                     KEIVETFGIESAPVNLYNRNVYDDAEESGRSIFEMTGSERDKKAEAEFEEFVDYLLSL
                     EEEE"
     gene            complement(58138..58914)
                     /locus_tag="K0W68_000067"
     CDS             complement(58138..58914)
                     /locus_tag="K0W68_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071758.1"
                     /translation="MSPSFIAEYSKVLSDFPECEAREKIRVGYMVNSDFSVLRLTEWS
                     LIGSNYDYCLTVSFVGDPDADWLQEHAMDLDEQGHSEEEIVHSVRELVGDEQEDDDGI
                     QIARFVYKRFQFRSQKGSHLGVQIKGAFVTPSKEQKGLARRVYNFLLNWHDHVVCDDH
                     QTVYGARIWAVGMLLVGRVQIYDNLNKEFIDVLVEGGIGQNGVKPWDALLLNSEAQLR
                     HWNPMAVSIDPSSQILTIISKEDRHIEVGVTTFDARSTNH"
     gene            complement(58915..59313)
                     /locus_tag="K0W68_000068"
     CDS             complement(58915..59313)
                     /locus_tag="K0W68_000068"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071759.1"
                     /translation="MHTALNASRSIQTIRDCTKMIASVPLRHWNDSLQIREMNKPGML
                     KDQRFIVNLKSDVEEGKFTIKLFAEMMVLNNRLRFLVKPEKQFPDVVRCEQDAFYEIE
                     SKIAKFIKDKYRVDVKIAKLDSSTDMRLAN"
     gene            complement(59639..59890)
                     /locus_tag="K0W68_000069"
     CDS             complement(59639..59890)
                     /locus_tag="K0W68_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071760.1"
                     /translation="MLGSLMIKLKVALITLAAVLFVLVGAYTMGGNAARRAMEEKAKQ
                     EDRKRLQSTVDVKNETLDELRRKDASTVHHELHNKWLRD"
     gene            complement(59892..60584)
                     /locus_tag="K0W68_000070"
     CDS             complement(59892..60584)
                     /locus_tag="K0W68_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071761.1"
                     /translation="MKTPRGIRNNNPGNLDKGSPWQGLVANPDEPRFCTFKDPVWGIR
                     ALAVTLITYHDKRRAKDGSSIDTIREVIERWAPPNENNTDTYINEVSKAVGVTADMII
                     DLHDYDILRPLVEAIIRHENGRGPLKTLNTWYAAEVIEEGLRRAGVVKPVKTVKAVPV
                     TKETAGATVTAGIGLAQLADVMPQVSAAMDKAQGHISSGDTVRIIFGIATIVVAGFIA
                     WSQVRKHQKGMA"
     gene            complement(60598..60921)
                     /locus_tag="K0W68_000071"
     CDS             complement(60598..60921)
                     /locus_tag="K0W68_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071762.1"
                     /translation="MANDVGGVTHDQVERIAKIVAREVVGKLGKEIREEIVHEVHEQL
                     KNYFGDMTPAQHSIQHSNLDKLLNRLDSISSGFFGGIISKVTSFIITALILGLAAYGV
                     KNGLQ"
     gene            complement(61019..61531)
                     /locus_tag="K0W68_000072"
     CDS             complement(61019..61531)
                     /locus_tag="K0W68_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071763.1"
                     /translation="MGVASGWVGSSAKSETGEQWMSAAGTKLGLGKPFMMSQMVGRSM
                     GCKIATAYYKGNPPDKVENWGAVGSDWPLAEKNQGTIAGASSCGLGRLVGIVLTQAHY
                     VPGLPPTGGLYIAGGRASNITVTVGGVSQVFTYNSVINGFHYYWQGTPSSAFITAMKR
                     TGVTQDLKIS"
     gene            complement(61617..64988)
                     /locus_tag="K0W68_000073"
     CDS             complement(61617..64988)
                     /locus_tag="K0W68_000073"
                     /inference="COORDINATES: protein motif:HMM:NF025253.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HBI3071764.1"
                     /translation="MWYREGTITFTQGSNTLVGAGTAWNVTANGVLPGMIVVAPDNKL
                     YEIKSVTNDTTLTLVENYTGETQSDIPCRIITTYEGDLTQFSARFTALLSRMSGDAKT
                     VRNWLTAVDEVTLEREDGTNVNVKPLSKIIAEHNKHVEWYENNTNAINSAGDKAKQAA
                     ASASAAAKSANLASSKATQASNSALDATASKNAANKSASSAKASETSALEAKNAAAIS
                     ASTASTKATEATSSANAALVSKEAAKLSEMNAASGAGSASTSANQARESARSAKTSAN
                     QASTYASAARASKDEASRKSAEASTSASNASSKSAESAAKAEEAKQSQTSALASAERA
                     EIAATRAERALSAISLHDASLTMKGIVKLSSETSSSSEEFAATPKAVKAAFDLAKSKY
                     TANDATTSQKGLVQLSSDTNNASESLAATTKAVKSVKDLADRKAAINSPAFTGTPTAP
                     TATQGTNNTQIATTAYVREAISGLVGSSPEALDTLNELARALGDDPDFATTMTNKLAT
                     KQPLDATLTALAGLTTAANKLPYFTGNDTAGLATITSVGRDILSKASVQAIIQYLGLK
                     ENGTSGDKIPLLSTANTWSAKQTFSNGLAGSLNGNSDTATKLKAARKIGGVPFDGSSD
                     INLPGVNSTGTQSTSGNAASATKLATPRSIGGMLFDGTANIDLPGVNKVGNQSTTGNA
                     GSATRLQTARTIGGVSFNGTANIDLPGVNKAGNQNTSGNAATATKLATARKINGVSFD
                     GSKNISITPKQLGAAKYVANLATGNTQEWTTAQFVAWLNSQGAFDATQWSTRCSWSYG
                     SNCYIGNASTGCGVIPLAGSVIEVFSTGTSNYTIRITTPSTSGGASGAVLNSEFIYTY
                     NGDNYSPTWRRNYSSQNKPTASEIGALASNGNAVSATKLKTARNIGGVSFDGTANIDL
                     PGVNKAGNQNTSGNAATATKLKTSRTIGGVSFDGSANINLPGVNTSGNQNTSGNAASA
                     TKLQTARTINGVSFDGTKNISISAAQIGALPIGGGTITGNLTVNGNGSFNDVQIRSDK
                     RNKRNLVKLDNALDRLEALTGYLYEIQLSDDSWKTSVGLIAQDAQKALSELVTEDTDV
                     ISGEKRLCLNYNGIIALLVEGFKTLRHEIKELREK"
     gene            complement(65083..69789)
                     /locus_tag="K0W68_000074"
     CDS             complement(65083..69789)
                     /locus_tag="K0W68_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HBI3071765.1"
                     /translation="MEQFKKKKLPLLIAGSGGKKSSKSSSRTPVEADDTVNSRAMASI
                     LDLLGEGVIGGLVNGARSIFVDDLPIVNEDGSSNFSGISWDFRDGSQDQTPMSGFDFV
                     ETPKSINIQLKKSHYVTVSIDNDEADRVRVIMKFPSLRRIDQKTGDTNGTTVEYKFQI
                     SNGDSTFVDVVAEGEKNVGIKLTAKKTGAYYRSYELKLPKPGRAYSVRVVRITDDNSG
                     QYLYNDTWVDSIGEIVDTPMNYPNSALVGLKVNSEQFGGSMPSRSYLVRGLKIRVPSN
                     YNEASNTYDGVWDGSFKLLSSSNPAWILFDLLTNSRYGLGQYVSESMIDLGQLYQIGR
                     YCDEEVDDGFGGKEKRFAINTQITSRQDAYRLIQDIAGAFRGMVFWAGGMVNIMQDSP
                     SDPVMMFTNSNVKDGLFTYKGSARKDRPSVALVTYNNKEDGYKQNIEYVEDQDAMRRY
                     GERKTEVVAFGCTSRGQAHRVGLWLLYTARMESDVITFTAGLDASFLMPGETVLIQNK
                     YRAGKRNSGRIVEFTKNSITLDAPVSLAKSGSFIRILNQEGKIVERDVLETGENITKV
                     TFSKALSSAETPVLNGVWTITEPDLEPMRVRIVNIAQGETSGSFDITAVENNPSKYEA
                     IDNGATLIPQNTTVLDPTYSKPSNLQITEGTYLSSPGNLSVKLTATWEGKSPEYWISW
                     RRSDENNVSNWQSARVTEEQYEIVNVAENGRYDFQLYAVSFNGKKTEIISTVYQVLGT
                     MTPPDAPTSLTAVGDYRNVILNWVNPDSVDLDHINVYASQTNNLDTAKLIAESASTTF
                     THSGLGDSETWYYWVRSANKRGMLSRPNSNLGTEATTRDVLSFLANKITSSELGQALN
                     EEIDSKASQQAVDEINNHIDQSVESLEGEISALNKELTSSIDEFKTSFTERSETLENA
                     QSELRTEVSSTREKVDDALRQVENSNAALIELRNTVSKQGDSVAGVVEAANAALENAS
                     SLIAEERKVRAQADLANAKKIETMRSSVDESVAAVEEMRRTVAEVGRVSSEITTNLEA
                     ISKTNIDLALRQDEDQHRQLVNNAKIATTQKILVDDVSAMASKVEEIRAEIGDNIRAS
                     ILEEATARTDGDNALANRVTQLQSKFEGDISAAIRTEQSARASANETLSKQITQLESK
                     VNTDISTAIREEQQTRATQDEALSRQITQLQTKVNNDISAAIKSEQQARVSGDSALST
                     QINQLQAKVDDDISAAINEEREARASGDSALSREVSSLKTKTGKDIAAAVAVETKART
                     DANDALSRQISSLTTRANNLQSSIERESTSRTSQDSAIAKDLASFKTKTAKDISAAVA
                     VETQARAAADTALTSQINSLKTQTGKDIKAAVAEETKARTDADAALSTRVTNLQSQTT
                     NYINAAITSESKARTSADNALGKRIDTLKASVDGNVAVIQQQASAIADANRKVSTSYT
                     LKMETSTSGGQKYVAGIALGIDTTGLSQFLVRADRFGLVNNVNGKITTPFVIENSVAY
                     MNGAYIKDGTITNAKVGDLQSTNFVRGRAGWRFGKNGTLEINGNSGGNGRLVINGQRI
                     DVYDDNNVLRVRIGRL"
     gene            complement(69806..70399)
                     /locus_tag="K0W68_000075"
     CDS             complement(69806..70399)
                     /locus_tag="K0W68_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBI3071766.1"
                     /translation="MSELVHVQLGGPMAKHFGRHWHLKVRNTKQALDLIEANKPGFKA
                     WMKRNIKTYDRYHIQITNKQGHKWSVDESEYQMMGQSDNIAKIRITPVPRGSGGSAFG
                     WFQTVVGAALLVVSAVMMPALAPLGLSLMMGGIAQIISPQATNESVRQADNSNSYYFD
                     GPQNTTNQGNPVQLIYGEEILVGSQVVSSSITIDQLM"
     gene            complement(70387..71184)
                     /locus_tag="K0W68_000076"
     CDS             complement(70387..71184)
                     /locus_tag="K0W68_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBI3071767.1"
                     /translation="MGNKAIPELGSDVMQQIYLCAINRYPNEACGFLVRTNGDKYRFM
                     EARNVSENPQNTFVMHVDDIMAAEDAGDVIAIWHSHTDESAEASDADRAGCEATEVPW
                     MILAIRKNVEGDAPFHFSEMNVITPDGFEMPYLCRPYVFGVFDCWMLCRDYLKREFNV
                     ELNPNAHLHIPSWYTGDNDILDQNYRNEGLVRLAPGTEPQRGDVFFIQYGKMPDHCAV
                     YIGDGMILHHQIDRLSCRAYYGGMYQKHTTHHLRHRDLLKGDETCLS"
     gene            complement(71177..71875)
                     /locus_tag="K0W68_000077"
     CDS             complement(71177..71875)
                     /locus_tag="K0W68_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBI3071768.1"
                     /translation="MGIKADIQSLSPSALIELFVLDMSNTTSGGKLFFHAGTNELMQP
                     VVWQGVTYEPWPIKASGFDKTGQGTLPRPKIQVSNFAGTVSAEVQANDDLVGCRIIRK
                     MTLARFLDAVNFKDGNPTADPNQHFPDEMWFIEQKTLETHQVVEFELSSVFDLMGVQL
                     PYRQIIKNTCPWKYRGPECGYTGPYFDKNNQQTSMSGADYCTKRYDACNARRNYFADG
                     VIHFGGFIGATRYG"
     gene            complement(71958..72293)
                     /locus_tag="K0W68_000078"
     CDS             complement(71958..72293)
                     /locus_tag="K0W68_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3071769.1"
                     /translation="MERQTFNWYPDYESEKSVKPNVTVLNFGDDYEQRQAQGLNRIKE
                     EWSLTFTRSYNEINAIDDFLTERSGVESFYWVNPRGKQIVVVCDSHTVKRYQGYCVLT
                     ATFRQVFEA"
     gene            complement(72336..76892)
                     /locus_tag="K0W68_000079"
     CDS             complement(72336..76892)
                     /locus_tag="K0W68_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBI3071770.1"
                     /translation="MSQNVEFILSLEDKQFTASIDRAGKLLTRFGEQVTKPAQKIQTF
                     ERSLGSVARIIGVLESKLDSTADKLQDVAVGFELASDATRKMRGNITSLNSGLKALIE
                     RVDTTTSSVDKLTASLRKVQSELNDFSDWATYASKSASRFGTEVKEASASVSGMNTRL
                     NTTTKRLSNWGVTTSQAAEGLKKVREQMDEVIGRQQLINKPVRVRTSGSGNGGGSGGR
                     GGYSWHIGKNNEGSMFSGLRGNIFLLGEIGDAARTVTSIMFGWQKPIIEAAAEMERMR
                     VMLRGLNKEKSNPGQAAADDMKYIVDMAQNAPFAMQALTDSFVKFRSAGLDPTDGSLK
                     ALVDSVARFGGDSELLKRAAVAVQQMSGKGVVSMEELRQQLGEAVPNAMQAMADAAGI
                     TMGELTKAVASGTVEAKQALSLMFVGLRAENENAAKDMMQTYTGALAQLQTSFTLFAD
                     RVGQAGYLDSLSKGMKELASIMNSAEGISFANSLGSGLTTAIDGLRQLAQWLAKNQEL
                     VINLGKVVAAMVAFKLMRAGIMGVVGAGSKMVSTFATMATAIQTPFNLGATAVTRFNR
                     AARMGLAPIPSLIFAIRGAITGLKGAFAGLTAFIAANPIGAAFTVATVAVAGLITYMT
                     MLRSETSKVVDEIRKIPEAMTAAKRAQMADRAEKLEKQILADQRALKTGEGVNYVSNS
                     AGVITHKESKADIEARLKKNQEEYQKITGTIALGDGAVAKRLAKEAAESQIEKIRAEN
                     QIFAATFVKARQEALDKIQKINDDGSLSDDEKNKLLAPLRETVNKSYLEPAQKLVDSF
                     SSRKNATEKQIATLNDQLEKAKKDGNTEQIQKLQGSIRGYQEHLEAVAQELTQAEFER
                     DNAAKTGKGVMSNQGTVLGLGTTDKAAQKALAQYMRNQMDSATYQRTLPDGTPMMDFE
                     GKPIIGPKQLKTQLNLQKASSASSLEKMSDEERAAAIAALTKAREQDAAAAEKAAQRS
                     ANASQRAAKKEQTAQQKLAAGYQKALDKADQLMGQMGESSKATVSFDQSLRDTTKSLT
                     DLANAVPNEFITQEMIDKAKKRLEDLKNATPEYREMFNRRNVEQMISTWAPESDSIIS
                     AGYTPSREEKVADFEDTYNRNLKALIELRDKASDPKVVALYTKKINQLIAAGNTALIK
                     ETGTATQKLALEYENLAEQIESTWTDLFSGLTDVLTDFVINGKMSFSSLSQSILKDIT
                     NMVVKSQITLPLMNMLGMGTTAAGSSQSGNLLTGVASAVANQGVRMGNTVNGDKSVGE
                     ATKETSSSVTGLGQTTQQTTSAIGTATNAIGSWVSGLFDSTEAKDAETKAVKDSIFSM
                     QNLSSVTGALSAAFAMLGANASGSGNKWLSFGATVASGLVSAWAGGGFDSLTSSSAKT
                     ATSSVADGTKGIPAIPKFANGGIFGKDGVIPLRAYQKGGIANSPQLALFGEGSMNEAY
                     VPLPDGRTIPVTLSTDGMSGGGNVLSPVSIEINVHSDGSTTESGDTESIWNNAAQRMK
                     AIALETIAQEKRPGGSLNPNTQRN"
     gene            complement(76900..77124)
                     /locus_tag="K0W68_000080"
     CDS             complement(76900..77124)
                     /locus_tag="K0W68_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071771.1"
                     /translation="MLSRNIDRLRAEEDVRNLQVARAAQADGEGVKAFMEGLQLRIGR
                     PVVTDKVYDPSQDRADPDAKEQLMQIFGRG"
     gene            complement(77250..77567)
                     /locus_tag="K0W68_000081"
     CDS             complement(77250..77567)
                     /locus_tag="K0W68_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071772.1"
                     /translation="MTKLLDLDSILPPKKSVKFGGQEYPIVEMTVGLFVSIKQMEGKD
                     LTNMSPVEQVTAYADLVRKVIPSVPDEVLEKLTVPQLQQIFTFAMEVIDEENEKAAGE
                     GAK"
     gene            complement(77635..78372)
                     /locus_tag="K0W68_000082"
     CDS             complement(77635..78372)
                     /locus_tag="K0W68_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HBI3071773.1"
                     /translation="MSNTHVKNIKLGACKVSFGGVDLGYTKGGVQVEVATETLKVTVD
                     QQGQTVISELVQGRNITITAPLAESVLKNMVDLMPGSTLSEDDNSVTITSAQGVNLIN
                     VAKELVLTPQDTTDYVLTLPKAATAGNFTMSYQSDDVRVFSVQFNAYPDDEGVLGKMS
                     GPKPVKNVTITPESPSVKVGATVQLTANFTPDDVVDKTGVWSSDNKKATVDHTGLVRG
                     VEQGSANISFTSNSGSKKVTKLVTVNQ"
     gene            complement(78446..78829)
                     /locus_tag="K0W68_000083"
     CDS             complement(78446..78829)
                     /locus_tag="K0W68_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071774.1"
                     /translation="MFIEAFANLMQKAKIGTVGTDIFCHYLPAYVKSGVLLINPNTGI
                     SIDHELQGFYHESFTIIVRGSSITTTIEKARKIIDMFPVEETESGEVYFRLVRPMAMP
                     VVYPKNDGSLIEAGIPVEFAGYLLN"
     gene            complement(78831..79304)
                     /locus_tag="K0W68_000084"
     CDS             complement(78831..79304)
                     /locus_tag="K0W68_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071775.1"
                     /translation="MGLKYDAHQFKRAGNRLNNSQKAFKRYLIRDMEKLARLVERLSR
                     AMAPLETGSLETAIFARVIKEGYSGLRIELSVSGAKPRQGHPGVEVGDYAKYMELGKY
                     RLGYLSRMKNVTNPPIEGVKPRVGPHFLERAVEISEKQFSEAILEAAKKAGFTRG"
     gene            complement(79295..79639)
                     /locus_tag="K0W68_000085"
     CDS             complement(79295..79639)
                     /locus_tag="K0W68_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071776.1"
                     /translation="MNISWQTEIAIYRFGTKNVYGEAQLQFVRKTNVGVVKFEQSNEK
                     SSVRADSSGSRGKASLELFDAVLVVPLESAVQLDDVLILEGQKLKVSSVHRRWGLRGR
                     PGHLEVGANIWV"
     gene            complement(79711..80544)
                     /locus_tag="K0W68_000086"
     CDS             complement(79711..80544)
                     /locus_tag="K0W68_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071777.1"
                     /translation="MAQIDSYRSGEAVSLSFAFNVLDIESATYTVKDSTGAILVDGEP
                     LEITSGQMSIPVVVSAEYNQLSEKERDLRYVIVKAVASGLTHEERQMYVLLNTFELSI
                     PEQSFATVADAQMQAIDMLNGDTLLSDGEGLMRKRLIEATRRIKTLPFSIRKILRIDF
                     DRYDRPQNMLNVYDIPWGADGAYRHDLVDWEKMTQEKFEEFPDYFKEALMLAVVNEAC
                     EIANGNDIVAAREDGILSESIGETTNMYRTGKSANVHVARSTWRLLIRYIDNRMIVRR
                     S"
     gene            complement(80544..80978)
                     /locus_tag="K0W68_000087"
     CDS             complement(80544..80978)
                     /locus_tag="K0W68_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071778.1"
                     /translation="MKPAKIRLLEPQFSDYSGMLCGVKFENGVSVSELPFIDQQRICA
                     SMRASTVEGKNVSPSAAYGERNDLNVDQIVEPSAPDIVPMKRGTADEPAKPIQTFTRE
                     ELESIADSEGIAGLRLIGNKIGVKAKGIVEMIDGIMKAQGGE"
     gene            complement(81076..81996)
                     /locus_tag="K0W68_000088"
     CDS             complement(81076..81996)
                     /locus_tag="K0W68_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBI3071779.1"
                     /translation="MPLLREEAEKLSNNELEQGVIETIIDRDDLFAILPFMKINSKAY
                     LYNREATLSEATFIDVNDTIAEGAATFSEHVAKLRILAGDVDVDKFLATTMSDTNNQL
                     AVQVRQKVKGLARAFRRNLILGNSETNTKAFDGIPRLMHKDQKIDIAGASMTFSMFDE
                     LVDAVKDLGADCIMMRSEHLRAYRALLRTVNVGPSEIMMENFGRPMLCHNGIPFIVND
                     FIPKNGVKVTEDSEQKTEGQEGTANIYCLHLSEENGLTGLYGGDNAGIVVENIGTVQN
                     KDAVRTRVKWYCSLANKHDKAIAALNNVKI"
     gene            complement(82022..82909)
                     /locus_tag="K0W68_000089"
     CDS             complement(82022..82909)
                     /locus_tag="K0W68_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071780.1"
                     /translation="MNLWQMLLARRGLMDVAEAHERGGAGGVAADNEQSTQDPDKQGE
                     QKEQPKGDDEYAGMTQEELLAELRKTKKAGAELLKENMKRKEKERTLADQLAQYGDID
                     PARARQLLEAEQAAENTRREAEQAELERRGEFDAVKKQMIEAHQAELAQRDERYAALE
                     SENASLKSQLVEMTVGASFSNSAFLRDKVLMTPAKARVIYGSHFEVGDDGSVVGYDKP
                     AGHKDRAVLVDGEGNTLSFESAIERIVRADPEADAVMRSEAKQGAGSNSKPTHKVVQQ
                     KSKSTMDKLTSGLGKIGLK"
     gene            complement(82931..84505)
                     /locus_tag="K0W68_000090"
     CDS             complement(82931..84505)
                     /locus_tag="K0W68_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBI3071781.1"
                     /translation="MELNDKQIKDLVARRHPEYEKKKEHWDFLASTYAGGRGWFTDNI
                     FRYFKEGDQEFKERVERAYRFNHTREVVNLINKYLFKEDIHRNIEEAPEQIRNFWKRA
                     TRQNASIDSFMAAIDLQSSIYGRIWVVVDSTMSGDVESVADEKKKDARAYAYWISPQQ
                     MLDVAWDDDGNMLWALIVEVARDDADPFTSTGQEYQRYRLWTQNEWYLFREEVKKGAG
                     GAGRRQAKVILEDSGEHNLGVVPVFPVDCIGESESPYFSPSLIDDIAYLDRAVANYLS
                     NLDAIIQDQTFSQLAIPVQSLLPGDENHTKVLEMGTKRVFTYDSEGGNQPFYLSPDPK
                     QAQMIITTIKTVINEIYHSVGVAGERTKQDNAQGIDNSSGAAKMYDFQRVNSLLVTKA
                     ERLERAERQIMLLVAKWMGVDLDEDHSLIAYPESFDIRGLTDEFSVAEKLSLLQAPDS
                     VRRHQMEMLIEKIFPNITEAMKKEFDKDLLNFPPKNDLNTLENKSVLTYDRGAAQESG
                     QDQPRGNGDSSTQENE"
     gene            complement(84532..85788)
                     /locus_tag="K0W68_000091"
     CDS             complement(84532..85788)
                     /locus_tag="K0W68_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBI3071782.1"
                     /translation="MAIPSSLSLVQLHSGQMKVFQSPHRFKVVCAGRRWGKSRLSIST
                     IIRAAAKEKKQRVWYVAPTYQMARQILWDDLQEVLPRKWVRKKNDTTMTIVLKNGSEI
                     ALKGADKPDTLRGVALHFVVLDEFQDMKADTWYKVLRPTLSSTRGGALIIGTPKGFSE
                     FHKLWTIGQNVELQRKGQWKSWQFVTADSPFVPTAEIEAAKNDMDPKSFAQEYLASFE
                     NMSGRVYYPFDRNVHVKPLQFNPRLPIWVGQDFNIDPMSSVILQPQPNGELWAIDELV
                     LFSSNTAEVCDELERRFWRWKSQVTVFPDPAGAYRQHARGESDVDIFKEKGFLRVDYS
                     KKHPPIADRVNAVNRMLMSASGDIRLYIDPKCKHLIDSLEKVIYKPGTRDMDKTGGIE
                     HSADALGYPVHRRYPVKNRVILGGSR"
     gene            complement(85788..86420)
                     /locus_tag="K0W68_000092"
     CDS             complement(85788..86420)
                     /locus_tag="K0W68_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBI3071783.1"
                     /translation="MTVEKDEVKTRLTPAEWAEAEAKWTSGEYTLSKLEEEYGIRRET
                     LSRHFKKRGLEKGADSVGKMVRESLKSDAELRAKARAEKIEERRTRYDGWAYALGQMV
                     MVEVTTAKREGKPLGAIEDSLKSLQRASNTLAKCFEVSSKALGMDHAENDEEEIPNLV
                     FGELTPSQVAKLRQEDDEPEIIDDELLETLEEEALSEFDATDDGSEGEDE"
     gene            complement(86617..86883)
                     /locus_tag="K0W68_000093"
     CDS             complement(86617..86883)
                     /locus_tag="K0W68_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071784.1"
                     /translation="MTLTDKQKDIIKTLNLGYERGHLLDLDELLEVLPYKTTKQSIQF
                     SIRALIKKGLVEKGHTRQRSDNRYHRRTLGLTTLGRAKAKLLVM"
     gene            complement(86893..87783)
                     /locus_tag="K0W68_000094"
     CDS             complement(86893..87783)
                     /locus_tag="K0W68_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071785.1"
                     /translation="MADIIIKRYRPEEFPRHLDFLERMTVTKGTVEDWHALKSLHYKT
                     DGKPFAPTYYRCELDDRLVGVVVMAYPKLLLAPRHRMFPKLKPTTNTTVANQYWGRYV
                     NNNFAVISRSVVDTQYRGVGVSYRMINLVSRMHDRPIIEIQSSMSKYNPFAMKAGFKF
                     IRPERPKSYESALRVFQRHFRSDPGDNEAIVKELFAMSESRRRRALRDLVADYHKNSS
                     LAKAGRNRGTTIQDIADSLVDEASIVKLLKDIHNLSFTSPLYGVYRNPDFGRRLPDTL
                     PLLAFDKQPLDKPLEIALPA"
     gene            complement(87789..88043)
                     /locus_tag="K0W68_000095"
     CDS             complement(87789..88043)
                     /locus_tag="K0W68_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071786.1"
                     /translation="MPDLKIVELKPSKETDNNNVEVIRLLEEALQHAREGKSQSLALL
                     MINNDGSVLDCWHNGGRPYVMVGAMESLRLDFINANIERR"
     gene            complement(88036..88674)
                     /locus_tag="K0W68_000096"
     CDS             complement(88036..88674)
                     /locus_tag="K0W68_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBI3071787.1"
                     /translation="MSKYIINVSFQTRVNKTTRTLEIAESFGLGLDEKEWTLYDNLEL
                     EVKQGDVVYITGQSGSGKSVVLRELQRQMKDEGLSVASIDDFTFDNEVNVIDQLGKTT
                     SDALGLLSMAGLNDAYLFVRKPSEMSDGQKYRLKIAKLIESGAKVWAADEFGAVLDRV
                     TAQVVASNLQRAARKVGATVMVATTHEDLKNALRPDMQITKHYKERVKVEYA"
     gene            complement(88671..89339)
                     /locus_tag="K0W68_000097"
     CDS             complement(88671..89339)
                     /locus_tag="K0W68_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HBI3071788.1"
                     /translation="MTKKFEIVYRDPADLIPYEMNAKKHDEQQIRDLAAAIKKRGFDQ
                     PITVDKNDVIITGHGRREAAIFAGLERVPVIVRDDLSDDEVRAKRLEDNRLASIDYDA
                     IKLQKELESLVLDDIEVFGFEERELNVLVGSMTEEMDTDSLVIDLGEETKRQKDEHTE
                     ISREVAAEEVRVVDVLGFKTLPAGSAIVVGDLLAHMEEMTGESGVDAFVAYAEKISSG
                     EMAA"
     gene            complement(89339..90019)
                     /locus_tag="K0W68_000098"
     CDS             complement(89339..90019)
                     /locus_tag="K0W68_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HBI3071789.1"
                     /translation="MDVKITILQVDVANLRPNTWNTNSVGAQNFEKLKGSIEKLGFFK
                     PILARELEDGFFEILGGEHRWRAAIEQGISTVPVLSVGKISDVVAKQMSLVDNERYGE
                     DDQIALQRFIEEIQSELDYQLSEIAPYDDEISMVLAKEAAIDLEALEALSRGSDEPVD
                     TDKREKTERVGAEHQTMRFKVTFDASDRVAETIKNIIKEQAINTGNEMENAGEALVWL
                     VDYYKERM"
     gene            90102..91661
                     /locus_tag="K0W68_000099"
     CDS             90102..91661
                     /locus_tag="K0W68_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBI3071790.1"
                     /translation="MTVRIVSNAVNALISGADDKVKQLVQQMLSYEVEAGDWKGTSTM
                     FNWSKNSFPAGFAKPVAANLNKAGIKCVHIRKDKAPALGKPNPAVNPFPYNPDYAYQD
                     QTVETLVREGMMIAQIATGGGKSNVACKAAARIGRMTLFLTTRSVLMFQMAENFQRSI
                     DYRAKNGEPWLKDQKVGVIGSGEFQVSRHINVATVQTLASFLEEPPRDATPDKKSYHL
                     KRRELVKRFLSSVSLLILEEAHESSGSNFYDIARLCVNADYRLALTATPFMKDSTEAN
                     MRLMAVAGRIEIKVTEKYLIDRGILAKPYFLYHKVAYKPDEARIKAELANKHLNFRVG
                     MSTAYQKAYQLGIVYNLGRNEAIVREALLYKQHSLNCMTLVRLKRHGQILMEMMKESG
                     LRVDFIYGESNQATRQAKLNSLASGEIDVLIGSTILDVGVDVPSVGAVILAGGGKAEV
                     EMRQRVGRGLRAKKNQANVCFITDFIDISNKYLLSHSYERKHIIDTTPGFAEGVLPID
                     GAFDFGVLKRD"
     gene            91664..91942
                     /locus_tag="K0W68_000100"
     CDS             91664..91942
                     /locus_tag="K0W68_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071791.1"
                     /translation="MSEKKTTYCQVALSDKANDKLGKFQMKLKEKNIKMSKAEVINTI
                     LETMTMAEFDKVSSAVGVSAKAREKIMRIYENSNMTKEDLEQILSKLP"
     gene            92118..92717
                     /locus_tag="K0W68_000101"
     CDS             92118..92717
                     /locus_tag="K0W68_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071792.1"
                     /translation="MKMKRSLLLACIYLSSFSANALSDIVSDMQKTYSMYTSEDVLKY
                     ESRGLIQPVWFRFSDNAEETILNAGKERLRLQINGTAVPENVNVSSDEAMKPSGIVLE
                     VQDGTLYCDEMATTGCQVNIRVGNEEPIVTYAKAEGNTSGKVLFIDENTSKRIVRDIQ
                     KTANSKSVDKKIYVEVPFFVVGNKEFKFGLLKTPLRVSE"
     gene            92714..93235
                     /locus_tag="K0W68_000102"
     CDS             92714..93235
                     /locus_tag="K0W68_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3071793.1"
                     /translation="MRTLDITYGIPAEVWPRDYEHAERTLLFWRSAKFPVRVTLEDGQ
                     VFCMYVYGLLSARNKLNLTPDPLSNTNRIRLPLERISTIESGIEEVDTSFTGRLTLNN
                     EQMVNQPSRRDFFKICRQAYQNGKSIRVYMADGREIEGTSDGVDACLVTLKLEDGRKI
                     LIFFDWVERILPF"
     gene            93533..94183
                     /locus_tag="K0W68_000103"
     CDS             93533..94183
                     /locus_tag="K0W68_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071794.1"
                     /translation="MANIIISKKSIIEAASIVSDELREKADLATQTYNEHYKNGTHTK
                     ADKANMQAATTKLAYFINNVVNAVEDEKLCSVFYYAIKASKQAPEVFFRDAMTNSYSL
                     EKLVYLVKSIKAGKCVYSVADMSGSRVFALIDMINDEIDTFTNGAVFDLMNEAKKACE
                     IKLDAGYTQANQLINLCERLGLVEKVKGMGSAKAGTQQYRFIKNDFYNYLADAFKA"
     gene            94233..94436
                     /locus_tag="K0W68_000104"
     CDS             94233..94436
                     /locus_tag="K0W68_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071795.1"
                     /translation="MVSYDKIRAEYRAKYRAYKLELINDLIAQRDQLNFTFSDLLNSK
                     RDCKRKREYLRLSALIGKLHNSI"
     gene            94457..94684
                     /locus_tag="K0W68_000105"
     CDS             94457..94684
                     /locus_tag="K0W68_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071796.1"
                     /translation="MVIFISGVNINNHTLVYDIAGLAGYALSSEVVDETTFKININDV
                     EHRARAGINEADVTLMLQEFLNAGFNIRLEK"
     gene            95068..95273
                     /locus_tag="K0W68_000106"
                     /pseudo
     CDS             95068..95273
                     /locus_tag="K0W68_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718033.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(95303..95785)
                     /locus_tag="K0W68_000107"
     CDS             complement(95303..95785)
                     /locus_tag="K0W68_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071797.1"
                     /translation="MNFIATVNAPAHGNIAVTFSDIEKRVLGAWRDNETVELSAQEKC
                     IIARDIIGNRRYSRVFEKAYVVNSGFGTFVFPVRSGRFCQSKLIEFATQISVWIKTQS
                     SFKFSDDEAVSQGMRIANNAIKCKNITYTAGVDTWKLFCANFMLNVYASNRIHILDGV
                     "
     gene            complement(96136..96510)
                     /locus_tag="K0W68_000108"
     CDS             complement(96136..96510)
                     /locus_tag="K0W68_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071798.1"
                     /translation="MGLDIYIETQPKNDLNNDASRKKVAYFRKFNALFGWMERNVGEV
                     KNCELLELTMNDICALKAHLMHMNESNCEEYLPTCEGFFFGSQEYDEGYWHDVGELKK
                     LVEELIKNHDFHNNRLTFCAWW"
     gene            complement(96629..97024)
                     /locus_tag="K0W68_000109"
     CDS             complement(96629..97024)
                     /locus_tag="K0W68_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071799.1"
                     /translation="MSVKRENLTVDVYYASESAEGKNVAKITVVTYNTETGTEVQAST
                     IVRKGDTSGGEYEAQYQSIFDATDPLLLKIESYFRKVNKEVFETMMNMVNTVFASSLN
                     TNTTWIGQYGLRITSGIPADTLIPESVFA"
     gene            complement(97151..97462)
                     /locus_tag="K0W68_000110"
     CDS             complement(97151..97462)
                     /locus_tag="K0W68_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071800.1"
                     /translation="MKKSLVLGLDKDQKRKEKPALVAQLTLLDIVANGTSIRLFRETA
                     VSFDKNTFTRYVMNVRRQRGKGWMAFQRMWPEHQLELALMEVNRVAQQEIQRASVMAI
                     A"
     gene            complement(97617..97946)
                     /locus_tag="K0W68_000111"
     CDS             complement(97617..97946)
                     /locus_tag="K0W68_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071801.1"
                     /translation="MFANIDINQIKKLTQKEFDQFYELEGWSSTLINSRWVLELMTRD
                     DAPALMICDMGEDADFMDMSEFCVDTYNRSQKYYFTCDSENDVISKVYLHLVQHWDVQ
                     DFLAIFA"
     gene            complement(98085..98300)
                     /locus_tag="K0W68_000112"
     CDS             complement(98085..98300)
                     /locus_tag="K0W68_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071802.1"
                     /translation="MKMPVTVEVWGVDSLAECLDAVGPELYRKLWSFVPAEGESPKGK
                     DIWHLLSEDEKRELVGAVHSEFPDDQD"
     gene            complement(99875..100060)
                     /locus_tag="K0W68_000113"
     CDS             complement(99875..100060)
                     /locus_tag="K0W68_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071803.1"
                     /translation="MLKEDSYLLKALEMSGVEPTVKNAHDIYLQFSTELKQLINKYGV
                     NDSSVKAMIDDELDHLI"
     gene            complement(100097..100396)
                     /locus_tag="K0W68_000114"
     CDS             complement(100097..100396)
                     /locus_tag="K0W68_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071804.1"
                     /translation="MLNITQAYQVALISSSHTSEEDARRFMIASNELDWVSQIEYGCI
                     VHAGLQDDDWKDELRRYGISEGAIANIQKVLDVGFNSVHFDCGAPVVEGLECWQW"
     gene            complement(100558..102591)
                     /gene="metG"
                     /locus_tag="K0W68_000115"
     CDS             complement(100558..102591)
                     /gene="metG"
                     /locus_tag="K0W68_000115"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBI3071805.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKCMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLTSELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATAAPVTGPLADEP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            102749..103849
                     /gene="apbC"
                     /locus_tag="K0W68_000116"
     CDS             102749..103849
                     /gene="apbC"
                     /locus_tag="K0W68_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBI3071806.1"
                     /translation="MSESKSPDALRAMVAGTLANFQHPTLKHNLTTLKALHHVALMDD
                     TLHVELIMPFVWNKPFEDLKEQCSGDLLRITGAKAIDWKLSYNIATLKRVKNQPGING
                     VKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQRPT
                     SPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDYLVL
                     DMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVENMS
                     VHICSNCGHHEPIFGTGGAQKLAEKYNTQLLGQMPLHISLREDLDNGTPTVISRPDSE
                     FTAIYRDLADRVAAQMYWQGEVIPGEIAFRAV"
     gene            complement(103887..104276)
                     /locus_tag="K0W68_000117"
     CDS             complement(103887..104276)
                     /locus_tag="K0W68_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HBI3071807.1"
                     /translation="MGFPSPAADYAESRISLDQQIIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCALDGEYRVKRYRKSPRQHLEDLRTGKKEALPKDDDGCT
                     GSNAVFGVITHIINDARSGEFDDCPVM"
     gene            complement(104449..104973)
                     /locus_tag="K0W68_000118"
     CDS             complement(104449..104973)
                     /locus_tag="K0W68_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBI3071808.1"
                     /translation="MTKFGKLEAHLLTRNYRYDVHPYRQWIDESEGVVTFPLYRFDGM
                     LAGYQTYRPGAPKQHSNPKMARYFTRAHGRQLLWGTYLPLKDGPIWITESIFKSAAVH
                     NAGGNSWALLGSTFSAGLRRQLAMLPYDFRVIGDNDAAGESLVKSFGKGFVAPDLDEL
                     QPNEVSHLIFSHSQ"
     gene            complement(104987..105841)
                     /locus_tag="K0W68_000119"
     CDS             complement(104987..105841)
                     /locus_tag="K0W68_000119"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071809.1"
                     /translation="MMHKSVAGEFQKEVDNTTVLLDDILSILALLETGDWSEHCTKTE
                     LGGRLEREITRLIGDAQEATVTSYELIAEAWRLMDGQDPKTSDWHSKASKYLNSNRVE
                     KVDDVKPKPVDHGFRDNCECSSCQTTARICSELTDKSSLIYEVNVGGNTWGECTRAAY
                     VRAKDNGELTRVVTNHPNNELKDHQIRELVNELRDIAVTYHGTQQLREKIARAVNNSL
                     KGGQSQPKRIRITHIGENGTEIPQFSVGDIFDVHGEEFGEPHITMPSGHVYWLRAMEK
                     YGDKWEVL"
     gene            complement(106052..106627)
                     /locus_tag="K0W68_000120"
     CDS             complement(106052..106627)
                     /locus_tag="K0W68_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBI3071810.1"
                     /translation="MTTITDKKQYPGEQYLNELITNIEFAARAPVEVVRAMAAELQKR
                     READSAEPASNHEELPLDYLQGQKDGLEWAAQLAEANHPQTGDWLYDDPLDLAKAIRK
                     GPDIPEFAGPHPVTPDGWISCSERMPEKGQNVLISVNFDSSLVEPLICSARYTGITFR
                     RGDVTIKPGNGIEQATHWMPLPEPPQESKSE"
     gene            complement(106629..107015)
                     /locus_tag="K0W68_000121"
     CDS             complement(106629..107015)
                     /locus_tag="K0W68_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071811.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AKPIGAFHIAEQQVDGTSDYLKDGEWPIDNGIIEVYAAPPVPVVPEEKPMPNPLSMYA
                     VDAVAAIAEVRGWNACRAAMLQGKGE"
     gene            complement(107026..107289)
                     /locus_tag="K0W68_000122"
     CDS             complement(107026..107289)
                     /locus_tag="K0W68_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071812.1"
                     /translation="MATLTKKERAWLNELQDVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDERILFPSSVESTAG"
     gene            complement(107291..107812)
                     /locus_tag="K0W68_000123"
     CDS             complement(107291..107812)
                     /locus_tag="K0W68_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071813.1"
                     /translation="MKFSKFSELVNRILSNNHSHRRDMDVTIVVHSPGRIGSTPSVEV
                     QSIQAGFDWDAGKVMIFPAQPLTTLTPEQVADITDSVRKGQSWHAYQEYKKHKEQLEK
                     LSMELEAAKQREKDLFMENVRLKSGIAGLIHLGIRYADVEVMKIAGDAQLSTPCTDSI
                     INSIAAGIRIKGE"
     gene            complement(107809..>108100)
                     /locus_tag="K0W68_000124"
     CDS             complement(107809..>108100)
                     /locus_tag="K0W68_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HBI3071814.1"
                     /translation="KAFHILERLEALATEEEISPEKLVEFSRVILRRKNDMERLTSGA
                     PSLSVRRTLCCSFCNKSQYAVKKLIAGDAVFICDECVDVCNRIIRGEKEGSA"
BASE COUNT        27683 a        26152 c        23167 g        31098 t
ORIGIN      
        1 ctgggtaatt gcagaatgcg atgtggtcat ctcactctcc tttgatgcga atgccagcgg
       61 cgcgggaatc attccatcgc tttacttctt cacgaattac gtcaatgcat tctttcgaat
      121 ccattaggta atcttcatca aaaagacgtt cctgttcgtt ttctatcgca acaatgattg
      181 cttcaactaa cttttgtgcc tgagaatcac tttctaactc tgctatgcgc ttacttccat
      241 ccgagataac gccctcgtaa tactcacgct gctcgttgag ttttgatttt gccaactcca
      301 gttgttttgt tagttccgca atacggcaaa catcgttgat acgcgtttcc tctaatgcgt
      361 tgatctcatc cagtagtgcc agcacggttt ctggtccggc cagaaatttg aaggcgttga
      421 gggcatcaat atcaacaccg ctatctttaa gttcctgttc acttaacaag tcatcatcag
      481 ctggcaacat taacaggcgt tccattgctg gaattgcacg ttccgccacc tcacgcagtg
      541 cctggtaatt aatttcgctc actggctgcc tcctgctttt ctgccttcaa caccatgcga
      601 gaaccatcat ccagctccca cactatctca ccaccttcag ccatgaccag ttgccacacc
      661 aattgagcag cctcattggt aacatcacga cctggatcat tgccaacgcg catacgtcca
      721 ccttcaacat cgcgcatttt tgccagcatg atagtttttg atagcggtga aaaaccaagc
      781 tgtagtcgtg ctgaattact cactggttgc ctcctttgcg aagctgggca gcaaagtcaa
      841 ctaaccactc agtcatttca accttcccta ccaggtctga accagggtgc atacagcaat
      901 cactctgcgc cgctttgaaa tccttatact catattcttg ggccaccaga ttttttgcag
      961 cttctatagc agcatccacc ccctgcgcac gtacttcagc cagaaaagca tcggtggctg
     1021 gagtctccgt taattcgcta acccaattat ctccgtgttc ctcggcacac atcgcatcga
     1081 atgctcgctc agattctttc agggctgcat tctccgccgc taatgcgtct ctctgcgccc
     1141 gcaacttctc aatttcggca gccatgtagt aaccaaccat agcgaagacg gcaaaagagt
     1201 tgtctgcctc atcaggcgac actgaggcca ttagcagacc atcgtaataa tcgtgactcc
     1261 cattggtgat cgccacctca taggagtcgt ggttctcacg cttgtgaatg agaactacgg
     1321 ggttttcgat tttgttactc atgtgttttt ctcttctttg ctgttacata agcactacca
     1381 agtgctggtt tattttcaca aataagaaaa tgtagaaaac aatttattta aggccacaac
     1441 ttatggcctt tatttattca tgccgaagga tttgtagcgt ctaattgccg tataaaatgg
     1501 ggtgaggtgc ttgccagtag gctccaccgg tccatgtgaa ccccatgcga ataagagtgt
     1561 ttattgcggc tttggtagac tccgattcga cactggcttt acgaaactct tgaacctctt
     1621 tcgccaacct gtatgtaatt gtgcagggga cgattgcagc ccagcctttc tcatgactgt
     1681 taatgaccat ctggatgtga ccatccagaa taggttgatt agacatgtta ttgaaaatgt
     1741 ctgcctgttt ttgaatgtga agaaaaagcc ttgccagctc catttgctca ctacgagaca
     1801 agggattatc aagagggctt tttgcaaaag cggctatacg ctgtgcgtca atgccaaaca
     1861 tcagcgcacc tcaccatctt tatggctgga gttaacttcc gccattaaat gtctgacgta
     1921 atcgacaaga gagccacctg gtggaatctg gcattcctca actaactgga agtagatatc
     1981 cgcagcatta cgtgtattgc tacccgcgca tattctttct tcccgaagtg catgaagctc
     2041 gttgatcaaa cggtcacact ctccgttacg ctgatccaca acagcctcaa gttctgcgac
     2101 gcgttcacct ggcgtcttac cttcttcgcg ttggatggta acgacgaaat cgccaattgc
     2161 agggacgttg taatccagct caaggtagtt tttggcaccg ctgcggacga actcacccgc
     2221 gaacatggtg gcgaacatct cagaggccat tgtgctgttg aaaagtgact ccagatctaa
     2281 cggagtcccg gcagccagag cctcttttgc tgtatccatt gtatccatga accgatcaaa
     2341 ctcattagcg cgcttttcca ggtctttcca ttgctcgctc caacgtttgg caatgaagtc
     2401 tatgaatgtt ttcgcggatt catcaaaatc cccctcaaac ttaacgatgc ctttatcaat
     2461 aatgatctgg cctaccgcat cctctgcata acctttcaat gtaaacttga aaggaaaatc
     2521 agaatggcct gtgacgttaa aatgctctaa ctggaggttg ttcatatgtt tttctcttat
     2581 cgtttagtta ttacattctt acacaaatta agtaagtatt tacctatcat ttaacgcgtt
     2641 tgaaaacgta tacgctgact gtgatccctg tgtcttcaaa ctcgttggta aacgacttcc
     2701 ctctggcata aacgtaatta tccatcgtca tccagtccag tattggcgca gtgcccggca
     2761 atactgctac aagacgcccg ccgactttca gatgccccag cgcagccagc gtgtgctctc
     2821 tatgacgacc aagcgagtac ggcgggttca taacgatttt gtcgaactga taacctgcgt
     2881 tgtcctcaga ccactttatg aagtcgcagc aaatcgtgtt cgtataccct tttccacgca
     2941 ggatatcagc gaagagaggt gcgatttcta tgcaggtaac atcttccaga tcggcgttaa
     3001 tataggccag aagatcccca cgtccggctt gaggctccaa cagcttctca ccaggcttca
     3061 attcagtggc tctggcaacg tactcagcaa tcaagcgtgg ggtagggtag aactggtgtg
     3121 attttgtatc cggtattaaa ccggtagcca caatcgtatt tagcgtatgg ccgatctcat
     3181 acgggaactg ccagtgcttt ttctcctgca cgccgccaat gaagctcagt gtgcgctcaa
     3241 gctcttccac ctgcgacttc tggagagctg aatcagagaa gtaccatacg cctttgtctt
     3301 tgctcaatcg cccgtcacga agcgcagtgc gaaccggcac ggagatcgtc ttctggatta
     3361 acccgaactg ctttggtgcc cgtgtttttg gcgcagttcg gcatggcgcg gggattgcag
     3421 caggcatact gtaagccagc acctcattca acttccaggc cacgtcagga tgtatttcaa
     3481 agtgaacgtt gccgttcttg aacatcttca cgcgcatcag atttccatcg acgttcatcc
     3541 agtcaccggt ctggcagtcg tttgcccgat acgcagctga tagcacctca gcagtgcggt
     3601 tgatggtaat aaattctttg tgcgcgaaga aatgaagcaa gacacgcaga tcgtcgatgt
     3661 agtcctcttt gcgatagttc acgctaacgc tgtcacgcca gaaatcagaa atgcagtcag
     3721 cgattatcag acgctcgctg aaaccgtttg tcttatttgt cttgtgcgca ggactcagcg
     3781 ccttaaacaa gccatacacg cgctcagaga gatatttgtg cctgtcattc agcaaattaa
     3841 gcatcgttgg tatgaccgtt tctgctttga actccggtac accaacgaac tctttaacct
     3901 tcatctgata gccggttctg tcagttttga tgacttcctg cttgccctcg ataaatagct
     3961 cacgccactc gtcgcgacgg gaagctggca tgatcagcag aacgttagtc atatccgtaa
     4021 ccttcctcca gtactcggcc caaatattct gtttcaccca ttccagatcg acgttatcca
     4081 gccagactcg attcaaacgt gagcgatctt catctggtcg gtggtttagt cgaagcaggc
     4141 gattaatcat gttgtggcgt tcatcgccat aaacgaagtc gtgtacctga tacatgaagg
     4201 agatctcttt ctcgcactcg gccacaattt cgtggatgac gttcatttcc tgtcgatagt
     4261 cgatagcagt gtttgagctg gcatcgccca tgatggaaag ggctgtattc ataattgcac
     4321 cattaagaaa tgatttgttt aatgatgcaa ttatttcaaa aataaaaagg tgctaaatag
     4381 aaattaacag gcaatactta aaataaaggc attgactaat catacttgga tgtatttata
     4441 accaggactt tatttgtata tagttttttt acattccatg taacattact tgaataccat
     4501 tcaccattat atatttggtt tcccgttgcg cctgtcattg ttacgtatac aggtctgtcg
     4561 taaggtgttc gtctatagtt atatactcca accattgcag gcattatcgc acacgggtat
     4621 cccatattta tggtaaattg aggatttgta atagtgataa tctctgcatc aaggggcatc
     4681 atctcaccgt gatataccat tttacctgcg ctattataca tggcaatacc atatcctgaa
     4741 tgtggtaaca ccatatcaga aaaagcataa actgttatcg ttccggggtt tcctccaacc
     4801 tgattcagtc tgagaacatt atacccatct ctaaattcat gaacgaacat catattcgcc
     4861 ttattactgc tttttataaa gaaaaaacat gatttgttcg acggtattga agtcttaaaa
     4921 acagaacctt tagacgtgga cattgtcccc ctattaatca aattttgcgg tgtaaattct
     4981 ggactcatcc acacattacc atttgactga gtaattgaca tcccgaacat tattgttatc
     5041 cccaatatgt atatatataa gatccaagtc ccttgtataa atttgaccaa cttactgtat
     5101 tgccgttaat agtgatagtt ggtgtaggta tatttacatg gttatcgtta tttaaaggca
     5161 tcaatgagca cacagcatgt aatgatttgc caggtggtgg atttgggtaa gtcttagagc
     5221 ctgacgtggt tgtaaatcga tcaagaaaaa aaataggagt aaggaagccg gtaacgttaa
     5281 ctcccttatt attgtagatc cctgtaccgt atgccatttt tcatccttgt ttttatcaac
     5341 ataatatggc cgatcatgcg gccatatcag atcaattaga gctttgaaag gctttgtgtg
     5401 gctgtgttgc gaggttatgt cgatggctta actcacgcat catgtcttca aggcgactct
     5461 ttgtgtcgtc gagttggtca gccattgctc ctaaaagctg acgaactacc atcggatcgt
     5521 cgctgtttag tgatggcatt ttataacccg cctgagaaga catcagattg aatgctgaca
     5581 tcaacatcgt tagagaggat ttaagcccgg caatttcacg atctttgctg gcaataatcg
     5641 tctcactttt gttactgtca tgagttcctt gagtctcgat caccatatcc aaagtctctt
     5701 gcaacttatc agctctctgt ttttcagcaa gataatgaac accgaaatga tgtgctaaag
     5761 ctacaacctg agttggctct tcaaaggtgg actcaaaatt aagtgctgta accactctct
     5821 caaaaatgga aacgtcttct attcctcgaa gaatggctaa cattttgaca agttcattag
     5881 catccatttc ttcgaatagg gcgttttgtt catcagctat tgctttgcat ttctcgcaca
     5941 tatgttttcc ttaatgctta aacaagttgt tttcttattg gctttattgt gatgtggcat
     6001 aaaaggggag caagctaaac gtaaaggtgc aaactctcta tttcggtcaa cgaaacacaa
     6061 tggccatttg acccttgtca ttaacacgtt gctttacgga ctagcggata agcgaagtag
     6121 atcgccagac caatgaggac gccatcagaa atgacagaca tcatctttcc cgtaaaatcc
     6181 accaacaccg ccatcaccag aagagcaatg acggtcacaa gccggaatct ctcaagcatt
     6241 agagataagc atccagtgac aactgaagcg cctgggcgat ctttttcagc gcctgctctt
     6301 ctggttcatc aatgccgtct tggtcggcaa tatcaaggca cagacaaagt acatctactg
     6361 catcatcagt cccggccaca tccgccagct cacgtaaagc ctgggcatta gcacggcgcg
     6421 gtgaggcttc gtattgagcg cggatattgg cgctcatctg ggctatctca ccagcaaaag
     6481 gagaaaatgc aggcagagct gcaattgttt tctcaagaat ggaaatttct tttgcatcgc
     6541 atgtaccgtt ggcataggag atcatgtacc caccccacac ggtggcttca accgcatcac
     6601 ggttctccat ttttttgact tcgataactg ctttacgagt tttctttttg aagaaaccta
     6661 acatgtgttt tccttttgtt attggtcaaa acaagttgtt ttctagttaa ataaattgct
     6721 tacgcaaaga cggtcaatgc atatgcacta acaaccgtca gaaataacca agtaccgaac
     6781 cgactggaaa aataaaaatc ccaactacac gagccaaggt catgccagcc tgaaactgga
     6841 gatcgccaga gcaaacaagt tttataatgt tcgataccca accggcggcc atgagggcaa
     6901 gaattgccag ccaaattttt ccaaagtgat ttgaaaacca gttcatacaa ccgccttagt
     6961 cacagcatga tcctgagtaa ctggatgaac ttgaggtatc gcatccaccg tcatcccatc
     7021 cagagtgaca ggtcgctcta ctggagtggt ggtaattgtc gtcgacggag gtgtggtgat
     7081 ggatgaatcc ggcatcaaaa ggctctgacc ggctggaacg gctaccgtcg ccagtgaaac
     7141 tataatgaga acgtaggcca ttggtttcag cctctttctt tgaaaatttt ccattcgaac
     7201 tgtccttgtt gtttttctcc ttcactaaag gccgtgcatt tttcgcttcg cgtgaagaac
     7261 cagaggcaac aatcgcccac acgttcgcaa caactgacgc ttgattatct aaacgttcat
     7321 tcagcaaccg aacggtagtc tctagttcat caaggcgtga cataacacga ccgctaaata
     7381 attcggccaa aatttgacgt aaagaacgag gacgtttagt tacagaagtg aaataggttt
     7441 gacgtgccat gtggactcca tccagtgtca taaagagttg cggctggcaa tcgccagccg
     7501 cctttctcgt tccatcctgg aactgtgtct taccgacaca ttgtcatcct gacgccgata
     7561 agatacatga tttataatga taggtaaaca cttacttact gtttgttata aacaaaacca
     7621 atatctttct tgtgaggcat atctgctgct gaaaaagctg cgatcttcgc gagtcgatca
     7681 catatttcat tttcacgatg cccggcgtga cctttaaccc acttccagcg aacattatgt
     7741 cgacttgcgg cctcatccag acgcttccac agatcaacat tctttaccgg ttttttgtca
     7801 gaagtcatcc atccattgcg tttccaccat ttcatccact gtgtcatgcc gtttttcaga
     7861 tactggctat cagagtgcaa aataacgttg catggatatt tcaaacgctc cagcccgata
     7921 agtgccccca tcatctccat gcggttattg gtggtgctat gaaaaccatc tgagaactcg
     7981 cgttcctcgc cacgatactg gagaacgata ccgtaaccgc ccgggccacc cggattttta
     8041 aggcaagagc catcactaaa gattttcacg gttttaagct ggggattgaa ctctacgaca
     8101 ggcgttgtgg gattggtgcg gggagaattt ttgtttttgt cttttttgcg agtttttgct
     8161 tgcggtcggg ctggtgtctt cgttgtcata taaactcctg aatcaagcgc cgcgccgatt
     8221 tttcctcgcg cgtgcgcaca cgcgtgcgtg ttaataatta ttaaaataaa caaattactt
     8281 cccagaacag gtttttataa acctgaactg aacgaacgaa gtgagtgaag ttcacctcga
     8341 acgaagtgag aggttgtctt ttcaggtaat actctcccag ggaggtgagt ataaaattcc
     8401 tcaccaacct ggtcgtttca taacctgaaa agctatggtc taagtctact gccagcttag
     8461 acttgggaag ttatggatga cagcacccca gaaccgagat cttcccacac tttatgaagg
     8521 ggagtactgg attcaacctc tcgaaacacc ccagactcga caatcataaa gtgacccttg
     8581 tctccgctca ctttggttcc cccttccccg acacctaaac ggcaccggtt ctacgctggt
     8641 agtgagcttt tttaaacctg acgccagtga cgcttacccc cacccatcag gtcgagtctc
     8701 cagtctacga ctggaaacta tcagatctta gcacttacaa ttcatcttat gaatagtttg
     8761 tacttatcta tcatttcgat tgattatttt ctcgaccatg tagttgaaca ttcaaggcaa
     8821 acacctcgtt gatcaactca cctaaaagct gttcaacaag ttcgcgatgc tccccaagat
     8881 gcaagcactt aattgcccac tcgtacaagt tgaaagcctg ctcacgatct ttcatcattt
     8941 cacgagcctg ggccagaaaa tcgctgtcta cgagcgcaac gacattagtc gggtatgaca
     9001 tgttggtttc cttaaagtgg ttcataaaat cgattttaaa gcgtctggga agggggtcta
     9061 gtggattctg tagtgatgtt caggtctgga gtctctgata caaaacagcc tgcttccgta
     9121 tattaataat taatatgtag ttatttatat atatacagaa gcaggctaat tacagacgcc
     9181 agaacaaact aagcagcctt ctttgatggt ctttttttcc tgatcacatt cgcaggatca
     9241 taccctccca gacttttcat gacgtccaaa gggatcttgc ttatagagtg cccagcgtcc
     9301 tgacagaatc ctcgaaaaac aacgagcata ctgccaccgg ggttgatgtt tacttctacc
     9361 tgacccaaat tgacgtcagg ctcgacaaac gctacgcggc caccagacag aataatcgtc
     9421 tcattcgcat actctgttgc acgctcatac cactttgtat cgagggattg aggaattagc
     9481 atcactgtag taactccgcg agcctgctca cgtatagcag catcaatcca gggggagatt
     9541 ttggagtagg gtggattgag gaaggcaatt gtacctggct gtccccattc agatttcaaa
     9601 gcatcacgct caatgcctat gaaattaggc agctttgcgt tgtccttgtt gcaggcaacg
     9661 tcaacatcaa atgtcactcc aagataacgt tgaatggcag aaaacagcca atcaggtgtt
     9721 ctccagaggt cacgaagaga gttgtcgcgc tttcgctgtt tgattttttc ggctgcaatc
     9781 atcttctttc tcgataggta agtaaatacc tatttttgca tttgatttta tgtatggcaa
     9841 tcaaataaaa cgcgccagaa atgcgaacaa gaagctctct ggcgcgtttt tttggtgctg
     9901 ggtatgggtt atcttaccca gcactataaa gctctctatg ggctttattt tcagtagggg
     9961 aacgtcatcc aatagtgtac tccgcgacaa acttcttaac gatcggtgat tcttcgttca
    10021 atgttagatt tccaccgtct ctaacggcaa ttcctgtagc tggaaataca gccatcattt
    10081 gcccagcctg agttgacgca gtgttaagcg gatacggttt ttctggattg ctcattaaag
    10141 caatcttaat actattactt gtagccttct tttctgtaag aaggtgtctc atggaaatta
    10201 ccgtgtaaac actaatatct ggacctccgc taaaccaatt aagaaggttt aatatcttat
    10261 ccttcgcttt gacaggcgcg ttttcgtaag cgtctaagaa aatatctttt tctaagcctg
    10321 ctttattgaa aaacgaatca tctttatcac ttaaagtaaa actaggacct cttgcagcac
    10381 gagttttttt atttgtattg ctatcatttg actgttgaac ttttaattca tcattaacct
    10441 caacacaatc ttcaacctca atgatcccca actgttctgg ctcattttcg gcagcttcac
    10501 cactttcagc gttaattacg tttataggct catgcgctaa ctccaactca tttaaagcag
    10561 agctgaaatc acctgctttt tcttcattga acagttcttc ggcaacatca tcagaactca
    10621 tggctacgcc agcatcgagg ccgagaagca gatcgtcaat atcatcagta ttttcaacct
    10681 gtgcagcttt ttttactggt tcatcaagtg attgcaacat ggctgtcagc tcatccagat
    10741 catcatttgt cattacattt tcaaccgaac tcatgtaagt ctccttctgt ttagttgcat
    10801 ttgcgtttcg atggctacat tttgactaaa cctatcaggc ggaaaagtat taaatacagg
    10861 caggaaatga ttgaaaggcg actgttcttt aaaagaaagc ctgcttcaaa ctctctttaa
    10921 agcaggcttt tgttttacac aatttcagag ggtaatttaa aaaatccgta tttattacgt
    10981 gccttaaaga agcattgcat cattaagttt gtgtcatata aggcactatg tgctttggca
    11041 tgatcgtata taaagccaag agagaaggcc aattcttcaa ggcgaggacg tttaccgtct
    11101 tctgtggccc acaatcccga taacatagta tctataacgg gaacgtctgg cagtcttact
    11161 ccataccctg aaaactcgtg tctaataaat ggtatatcaa acgcttcgcc gttatgagca
    11221 acccacgcac tacatgcctc catataagcg ccaatttcgc tagcatgatt tgacaacaaa
    11281 ggttcagctg cgagctgttc taatgaaatg ccatgaacgg cttgagcttt cggatctatg
    11341 tttctgcgag ggttaaaacg catctccaga ctatcaatat gtctctgtgt ctccagttca
    11401 tagcgggtaa ttgcgatttc aataatttta tgaccagcaa ggaaatccag tcccgtagac
    11461 tcgatatcga tgcctgcaac aatcgttgtc atcagaactc cttacaactt cttagcgccc
    11521 ttaagtagtg cgctacgtac aaattgagcc gcttttttca aagtttcttc actggtttca
    11581 cagacaatcg gatctttcca cttacctgac gcagtgttga gaatattgat ttggttgcta
    11641 tccagacaaa cagagatata aagcaccgtg ccaccagcca gcatcaaatg cataggaaac
    11701 agaggctttt tactgtcttc actgaactga gacatggcga tgttaatggc ttcacctact
    11761 tgtgcgccaa caagcccctg cgcagtttcg aatatggcac gaatagccag acgagcctct
    11821 cgatcgctca taagcgaccg ggaatgttcg tccgctacac gaatcaaagc ctctgttgtt
    11881 ttccgatcca gttcatcttc aagcaacatt tcactaaaca ttttttatcc ttaacccatt
    11941 ttgagagttt tattgtcgca atgtcgaaga ggcgaacaat ttctaatgaa cggtcactcg
    12001 accacagaac cggttttcta tcttttctac agcgttatct agcattctca tcactgatct
    12061 ggctcttgct tcaaccgtgc gatatcggtt ggaaacgacg aaggtttgca gatcccccct
    12121 ttgcggatgc aggtgaattt ttgtcagttc cccgcacatc aacgcatcaa tacgagcgac
    12181 atacagacgg tctaatgatg ctcgttgtga tcgcaagtaa tctcgctctg agagagcgac
    12241 tcccgggcgt aggccagcaa tggcgttaaa gtttgaaata gcagttttgt ggcaaaactt
    12301 ttcgatatcg agggctaact caatacatcg atcttcattg gtatggccga ccaaatccaa
    12361 cgtgtaggcc ataacatcag caggtgtgcg atcgataaca aagccttcaa caccacgcgt
    12421 aagtagctct atatgctttg cgatttccat ttgaatctga agccgctcat atagaggcat
    12481 agactcacct actcgaacac cgagtcggct catcaaactt ccgacaccag catccacgta
    12541 agggattccg taatgtttgt cgatatattt tgccagggtt gttttcccac tgccctgagc
    12601 accagtgatc ccaatccggt aatccattac gaccttctgt atacgatctg cgaaaagcca
    12661 ggctcttcct cgctggctcg ttgggtaaaa actgtttcaa caggcacaaa gcctaaatta
    12721 cgcatcatgc ttgccgggaa gaatgcatcg gcgtttggca catcaacgcc gatgtgggag
    12781 agccaaatct cttcaacatg cggcatgaat agcgagtaaa tttgttcccc gccaatcacc
    12841 cacacaggct ctggtaatcg caacacatca tccacaccag cagggtagaa tccatttggt
    12901 acaaagccat gagagcgcgt caaaacgagg ttatgacgct ctggaagagg gcgtttgaga
    12961 ctttccatcg ttttacgccc cataacgaca gtggcgtttt tggtgagtct cttaaatagc
    13021 ttcagatcgg tagaacaacg ccagggcagt tcgttcgcta tgccgatctc atagttacgg
    13081 ccaacagcag ctatcatctt catttgctca cctcatagat gaccggtcta tgatgagagt
    13141 cagctaaagc ggcacggagt cgaggatcgt gcaccagcgc agcgataagc agatcgcctt
    13201 tatgctccgc taatgtgcgc ttaatatgct tctcataact ggtgccattc ggtacgagat
    13261 gaagccagtc gtaatcaacg ccaaaatctt tcagccatcg tttagtgggc gcttcaagag
    13321 tttcagagcg gctactgata agcaccactt cagcccctga gcgagcaaaa ccacgcaaca
    13381 tacggctggt ggggaaaatg agttcatcac cggcaatgag ctggcctgca tccgtgtcag
    13441 gtacagattt acgatggctg gtatttgcca gcacaccttc aatttcgcat aagacataca
    13501 tacctcttgc catatcacac cgccactgga accttgatcc acggtagagg gtcatagcca
    13561 agaatcagaa tatcgtccca tttgaaatca tccagctcag accattcatg agggaaaata
    13621 acgaccggat ctgaatgctc tggagcctgt cgtttcatta gctcacacac accttccatg
    13681 tggttgttgt acaaatgcac gtcaaagcca aaatgcacaa atgcaccggc catgtgaccc
    13741 gtaatcttcg cgagaaaatg agtgagaatg ccatagccag caatattgaa tggcatacca
    13801 acaaaagtgt ctacgctacg ctgtacgagg caggagttga ggatgcgttt ggggattcca
    13861 agttcatcca gttcagtttc agtaacactg ccgtccatct ccagatcgta tagcatcttg
    13921 gtgtagatag actcataacc gaagcgactg tgttgaagac caatgtcagt tgccatcgtt
    13981 aaacgggttt caaaatcaag ctcacgactc cacaaagaga agacaaagtg gcaaggtggc
    14041 agtttcatat cctcaagctc gcctacgttc catgcgttaa gcatgatgcg acgatcggta
    14101 gggttcgtgc gcagtgtgtc gacaatacgc tgtagctgat cgatttcacg ggacagcact
    14161 acgcgatctt cactgatacc caggtatccc tcgactttgt acccgcgttc acggaaagta
    14221 gcgatcttgc tcagatattc actatggctc acgatgcggg tatcttccca tcgacgccac
    14281 tgcttaccgt aaaccggccc caaatcgcca ttctcatcgg cccatgcatc ccaaatctta
    14341 acgccgttat ccttaagaaa ttggatgttg cccgtgcctt tcagatacca ctcaagttcg
    14401 acaagcagtg gcttaagatt taccgccttt ccagaaataa gcggcacagt cccaccagta
    14461 agcatgtagt aagagggaac gtagcaggtg cttaaagtac cagtgcctgt gcgatcatca
    14521 gtctgcacgc cgcttttaag caccgtctca ataacttttg catacgatgc gtttgttacc
    14581 tgaccgttcg tatgctcacg attcaaaagg agtgacataa aacctcacga agataagtaa
    14641 tcaattattt atcacgtgag tttacattca ttgataaaac aagaccagat agataaaaaa
    14701 tggtggccat aggccaccaa aaagaatagt cgttccagag ttttatacgc aaatcaaata
    14761 tacattagta gataatcacc tacaattatt ttttgatcat tccgtacatt gctgaggctt
    14821 tagcttgctc aacaaaggag gataaatcga catcactata ggttggagac ttcagaatct
    14881 ttccatcgga aattcgaaaa ccgatcatct tatcggtgcc atcagcatgg cgaaatccca
    14941 ggtctgaaga gtcgtatttg cagttggcca cagcctggcg acgttcctct acatcagcag
    15001 gccataattt tgtcatgtta gaccgatgaa tttcgccaac cagctcgact acattgatac
    15061 cgaggaaatc agccagtcga taggtcatca tgcacgccac atagattttg ttcatgacac
    15121 ggcgcagttc ctgaatcagc tcagaatcct taacagtctt gttctcaagt ttatctgcca
    15181 gctcttcgag catacatgac gcctcacgag cttcctgaaa tggcattgcc atatcatcga
    15241 aaacagtgtt accgggcaca aaaattgtct gcataaagcg atcaatgctc tgttcctgcg
    15301 tgtaataggt catgccggta gaaataccac ctttgatggc gaccatcgta ccaacaccaa
    15361 cgtataaaaa gtcggccatt gcatccagca gttgctcaat atccccattc attgcagctg
    15421 gaataccttc cactacagct tcttcatgaa tcaggcttgc acggagacga agcagctctg
    15481 gtgtcggcat aacgcgtctc ggatgctgga agagttcatg aaactgatcg accatcttgt
    15541 aaatactttc agtagctaca ccatgacctt catgcaactc atatggttct ggcttaaatc
    15601 caatgagacg atcggtggca agtttcaggt ggtcagtcag tttggtaaag ttcatgctag
    15661 ttttgttcct tcttaattct tgtgatccat tgtggcccag cgttgccagg ttacataatg
    15721 ctttgtatcg gttcacaggt ctgcaaactg gcttagagaa gccttgtcca cggtagaatc
    15781 gatctgacct accagatatg tgctttgctc tgcctcttgt ggtgcaattt gcagtgtgtc
    15841 ggttaacaac cacttgttca tccagaccag cggatcggtg gtaacctctg gaaataaagc
    15901 cttcaggcca agacgattca tagccagatt ggtgcggtat tttacatagg tcttcaaaat
    15961 atccgcgttt aaaccaatca tcgaaccgtc tttgaagaga tactcggccc agttcatttc
    16021 ttgttcggcg actgatttca tggtgtcgta aatgacgttt tcttcatcag cagcaatctc
    16081 tttccacaat aaaccttcgc gaccggtacg catgaagcgg atcatgcgtt cagttccttc
    16141 gcaatgcaga gcttcatcac gggcaataaa gcgcataatt ttggtgttac cctcaagcaa
    16201 cttccgttcg ccaaaggcaa acgtacatgc aaaacttaca tagaaacgga tcgcctcaag
    16261 cgcattaatg gacaccaacg tgcggaaaag ctgacggtgc aaaggatatg gctcccagcc
    16321 atattcattt acgtacagac gctcaaattc cttctcgcct aacgactggc gctcgcaggt
    16381 catcccataa agcctgtcat attcagcaga gatactgatc gctcggttga tgatttcttc
    16441 atccgtaaca ataccgtcaa aaacaatgct cggatcgtcc accatgccac gaataatgtg
    16501 ggtgtagctg cggctatgga tagtctcaga gaaagaccat gtttcaaccc acgtttccag
    16561 ttcaggaata gagataagcg gcagcagcgt tgcatttggg ctacgtccct gaactgaatc
    16621 gagtaacgtt tgataacgga gattgctcag aaaaatgtga cgttcgtggt cccgcagctt
    16681 cgtgttgaag tcgatacgat cgctcgaaat atcaacttct tccggccgcc agaagaaaga
    16741 caattgacgt tcaatcaact tttcaaaatc acggtatttt tgctgatcgt aacgtgccac
    16801 gttgacagat tgtccgagga acataggctc tttggttgca tcattagcac ccaaacggaa
    16861 cgttgaatat gacatgtgtt tttccttttg ctttttgatg tgtttaaaca agttgttatc
    16921 ctatttgttt aaacaaggct tacaaatcaa agagaaggtg gggatacacc ccaccatatg
    16981 tcagattttg caggctccgt cgcactcatc ttctggctcg acggctacag cttgcgtcag
    17041 tggattctgc ggttcgtcgt catcacgctt tcctgcccca tctctggtgt tgtggtagta
    17101 aagcgtttta actcccatct tgtaggcgag aagcaaatct gacagtagcg tcatcattgg
    17161 gactttatcg ccttcaaagc gagaagggtc atagttggta ttggccgaaa tagcctggtc
    17221 aaagaacttc tggatgatcg ccacctttgt cagatagccg cggttgtccg gcatatccca
    17281 caggtattca tactgttcct tcagtttttc aatctcaggc acgaccatct taacgatgcc
    17341 gtccttagaa gatttcaccg acaccggtcc acgaggcggt tcgatgccgt ttgtggaatt
    17401 agtgatctgg ctggaagtct cgcatggcat ttgtgccgtc agtgtggagt tgcgaagacc
    17461 gtattttgca atacgttcac gcagttcttc ccacggcatc tttaactcaa agttggtttc
    17521 gccactttcg tctaatgaag aacggtaatg gtcgatcggc aactgaccaa ttgcgtactt
    17581 ggtctgctca aaccagtcac actcaccttt tgcttcagca agttggcagc tggcatcaag
    17641 aaggtaatac tggatagctt cgaacgtttc atgcaccagt ttattaccag cggtatcaga
    17701 ataacgaatg ccattctttg ccaggtaata agcaaagttg gttacaccga tccccaggct
    17761 acgacgagct ttggcaggca cttcggctgc ctccatcgga tagtcttgat aatccagtaa
    17821 cgaatccagt gcagcaacgg catagaacgc cacctcttta agagactcca gtgaacggat
    17881 ggctccgagg ttaaaagcgg ataaagtgca tagcgcgatc tcaccgttcg ggtcgtcggt
    17941 aaatgccagt ggacgggtag gtagggtgat ctccatacac aggtttgact ggcggacagg
    18001 tgcaagagca ggaatgaaag cgccatgctc attaatatga tcgacgttcg caatataaat
    18061 tcggcccgtg gacgctcgtt cctgcatcag agatgagaac agctcaatgg caggtactga
    18121 cttcttgcga attgaatcat cggcttcgta tttatggtac agcttttcaa acagctcctg
    18181 gtctgtgaag aaggcttcgt acatatcagg cacatcatgc gggctgaaca gagtgatgtt
    18241 ctcgcttctg acgagtcgac ggtacattag acggttactc atgacgccgt aatcaagatg
    18301 gcgaacgcgg ttttcatcaa taccacggtt atttttcacc accagcagac tttcaacttc
    18361 aagatgccag atcgggtaaa acgctgttgc tgcgccacca cgaacaccac cttgcgaaca
    18421 agatttaaca gccgtctgga aatgcttcag gaatggaata actccggtat gggtggcttc
    18481 accattgcgg atctcgctgc ccagcgcacg gatacggcca aaaccaatac caattccagc
    18541 acgacgtgaa acgtagtcga taattgcggc tgaagctgca ctaatccctt tcagactatc
    18601 gccagactca atcagcacac aactggagaa ctgacgggtt ggagtacgga cgccggccat
    18661 gattggtgtg ggcagagaca gtttgaacgt acttgtaacg tcatagaaac ctttgaccat
    18721 ctccatacgt gttttgccag cgcagtcgtc ttcccaattc tggaacagac acatgcctac
    18781 cagcatatag atatgttgcg gcccctcatg aatctcacca gtaacacggt tttgaacgag
    18841 gtatttgctt tgcaactgca ccgtagctgc atagccaaaa agatcgtcac gtttcggctt
    18901 aatgtaatta ccgagttctt cgatctcttc aaacgaataa tgtgtcagca aatccttgtc
    18961 ataaacgccc ttgttaacgt tcttgacaat gtgttggtag aagttcggat agtcgtaacg
    19021 accgaaggca tctttgcgga ttttaaaaat gttcaggcgg gcagccacct gtgagtaatt
    19081 tggtgcttct ggtgagatca aatcagcggc agatttaacc aaagcctcat gcaactggct
    19141 ggtggtcatc ccatcaaaaa tgctggccgc agctcccatt gctacagcgg atgcgcttac
    19201 accacgaatg ccttctacgc catacattac gacgcggttg tatttttctt cggataacgg
    19261 ctctgtttgg ccgttacgtt ttacgatgct tatcatgtat caccaaataa agagggccac
    19321 taaatatagt ggcccatgtt aatagataag cacttaccta tcaatgaaat caaatgagag
    19381 acttaagaac atcgcgcacc tggcggaact gatctgtttg cataccggta tggatggcag
    19441 ccaccgcatc agccaaatgt tcgtttttgt tcacgagaac atcctgtcca gattgcttac
    19501 gacgcagcca cggtgcttta gagtgttttt tcgtagccca ctggataatc tcttctttcg
    19561 atgtagtaag tttattgccg acaaaatgct tgatttcgtt tggagtaacc tggatcaatg
    19621 gcttatccac acacgcaagt acaccgatac aaataccgta agacgtctgc gaacgagagt
    19681 tttggctccc aaccggtagc tcacaaaaaa ccatatttgc cttatcaatg attggcttcg
    19741 caacacgcca tatttcactg gctcggcgca gatcgtcact gttcacacga acggtctttt
    19801 tgttactccc cgctttagtc tcaacaagag taaggtcgtg aatctcaagt tcgtccgttt
    19861 caaggtctaa agtgcccaca gcaagcccaa agttgctcat tgagggatca acaccaacta
    19921 cgctgatttt tttactcata tagtctcctt accatgttcc ccatataggg ttttccaata
    19981 atctcgcctc caacgaattg gttactattg ccgtttgcaa cgttggttgg ggagagtttg
    20041 caaagtctga atactcattt tcctgcgtga cacgtttatc tacggcatgt ttaacgccac
    20101 ttgctatgaa atgacgcatg atgcccaaca gcctccgact gtctgcacta tcgtttgcta
    20161 aaacatgcgg gagctgaagt tcgatttcta ccatttctcc atacttacca acaaactgta
    20221 gtttgtccgc actgtacttt ttatccctcc tttggataag gaaaccagta aaagcatcac
    20281 ctggcgatgc caaactatcc acccgcttat tggcaagtcg aataaggttg gtgaaagcca
    20341 tacagtgatg cgcacccatt cccccaaaga acccttcgct attaatgtta agatcttcat
    20401 tttcatggga tgcaataaac ccaggcccga cgttgggaag atatcccccc tcaacaaaag
    20461 gattaatcag gatcggcgca atacatccag gtggtagtga tttcaaactt tgcatcaatg
    20521 gcgtctcgga tattagacca agcaacgcgg caaattgtac tccgtcaata ttgttgctgc
    20581 cccacagaat acgtggagcc gcaccattgg aagtatgccc attgatcagt tttatcgact
    20641 gcatcactgc aaatacctgc aaaaactgcg agcggcttaa tttaaacgac atagctgcgc
    20701 ccctctttaa cttccagcgt aatagtttcc cggaaccatg acttcatctc tttatgggag
    20761 ataatcagca cagtacctcg ctcgcgagct ttggactcca gaatacccat gaggcgttca
    20821 agaccggctg tatcgagtgc atcgtcaatt tcgtcgccga taaacaaatc gatgttttta
    20881 ctcgcccggt tactaaccag atcctgcaat gccaaagaac acgcaatgcg aaccttccgc
    20941 ttctcaccac cagagagggt ctggaacgac ttacttgaac ctttcttctg cacactaatg
    21001 ttgaatttgt cgcgatactc accttttttg gtgacatcca tcgtcgacca ctcagcagta
    21061 atattcccgt cagacaacgt attgagatac tcggcagtgc gtgtattcag gaaaggcgta
    21121 acagacgtca aaatatgaga acgcaccccg gcaggagagt agacctgacg agctttttcc
    21181 aatagcaacg tttgttcctg aatagccttc aactcatctt ttaaggactt gaaggtagac
    21241 ttactggata ccaggttatc ttcgtgtctg gcgataagag caataaacgg attaatctct
    21301 tttgatatac gatcgacctc agtacgagca cgagtcacaa gagactcaat agcaacaatc
    21361 tccttctcac gatgacgcaa agatgagagt tgtttcgtaa gctcttcaat ccgggcaatg
    21421 atagccgtta catcaggtgt cgttttaaca agggcagact caatgcttaa cgctttctca
    21481 agattcgttt tgtgtttagc cattgcctct gcaagtgtct tcgcctgacc aatttcctga
    21541 cgtgcttgtt caatgaaatt ctccttcacc gttgatagat cttcttcgca gtaggctttg
    21601 ccacaagtag ggcatggtga tcccacttta gtaccaacct cttctgcttt ggtcttaaaa
    21661 ttacgcgcgc ggttcattgc atcagcctga ctattttctg tagctttgat gcttgcccga
    21721 atatcagtta tcgcaccacg aactttgagc aacttggcgt catgttcttc ttttgaggct
    21781 aactttttgc gctcactttc gattgccttc tcggtatcac ggatctgttc gggaagagtg
    21841 cggatctcaa gttcaacttc cgttaactca acttcggcac ttgccagccc agccagggca
    21901 tcgtcataac gtttagaacg ctcttgttcc catgattcag aggaggcttt cgcggactca
    21961 atttctgact gtgttgcctc aatggtcgag attgtcgact ccaatttggt tttaaccaca
    22021 tccatacgtg cggcagctgc attagctcgc tcacgagcaa tagcgtaggc gcgtgtcagt
    22081 ctgtcaacgc cagcggcttc ttctacgatg gttttgaggt ttttgtcgga cattccaggt
    22141 aaatctggca tagcttcttg gctcgcatag atggaagcca tgaaaacctc tttagatgca
    22201 ccgatcagac gctcgacgaa ctcttgcgtc agcgcatctt tgcctttcgt catatcgccg
    22261 tcttcaccac gaacgataag ccgatttttg aactctttgt gtttacggtg acgaatgatc
    22321 gcatatctct tgccttcatc ctcgatggtt actgcaacac gacagttctt ttcatggtcg
    22381 gtagacaaca catcgtcacc cttcacacca tgagctgttt cgccataaag acaccacatc
    22441 aggctattca ttagcgttga ctttccagag ccattacttg atgcggaact atcatcacta
    22501 ttaacaccct gaatgagcac taaaccacgc tgatctaact cgacctccgc gctggcgata
    22561 gccataaaat tctcaacctg gagctttaaa aacttcatat aacacctttg ataccgtgtg
    22621 ttagcttttg accttctttc aatctgaact ccgtatgcgc cgggaaggat ctgcgcctca
    22681 atacgccttt tctggttaac cttccaagat aaaacgctac gggatttcct tgtggcgggt
    22741 agggcttgtg aaacatcacc gtctggcctc gttcaagtct tttcatcgtg atggcaaagt
    22801 ctcgaaagct cccataacga ctttgtctca cacagcctcc gaactctccg cttcggtgag
    22861 gatttcctga cacaaaatat ccaacttgct cagatcaaat ccgccgtcag tatcgtggac
    22921 aatcttgcaa taagcagata ccgactctcc caggctatcg attttgctgg tttcagttgt
    22981 gctggctgtc ccttccatca ttgatgactt acggatgaag ttgcacacga caccttttgc
    23041 gcccattgtt ttgaggatgt tctggtactt aatgccttct tcatcgttct caattacggc
    23101 gcggaaacgc acgtagttgc cgcgaatttg gtcatcagca acatcatctt ccaggttaat
    23161 gaatttaggc gcactggttt cgtagtgact gaaactgccg tccgggttta cgatcatgta
    23221 accagccaga gatccaacat ctccccaatt ttgatgggtc agtgcaccga cactgataac
    23281 tccaggaatg acctctttgt ggttgtggta atgcccacta agcacgagac gaaagccgat
    23341 atcctttaac tcctgcgcat cgataccgac gtcaggcatt gttggaatgg ctttgttaat
    23401 ggatgtatgg ataacaacgt catgattatc accttctacg ctcttacgta atgctttcag
    23461 atcgctgata agctccgcat gattgttacg ccagctaatc aggtggacag tcacatcacc
    23521 aatttttatt gagtgtgggc gtttgccaca tacgattacc acgccgatag aactcagcga
    23581 tgctgctgcg ttggcgctat atactgaatc gttggtttca agatcgtgat taccggccag
    23641 cattactact gtcagatcaa gctcgttgat aatccacttg tacgtttcag ttacgtaatg
    23701 caaaacagaa ggggacacag ttcctcggac gtgaaatgta tcaccggcaa ccaacatgta
    23761 cttacaaccg gccttcttca tggcgatggc tgcttctttc gttgcttcca actgtatttc
    23821 aagtctggag ttaagcccct ctgcgttcgt cgtggagaac gcatcccatt tgtggtagtg
    23881 gcagtcagaa atgacgccgt atggcaaagt catgtgtttt tcctttgtgg ttattttgat
    23941 acaaatttta agagcaatga gaaggtaaac aactatgcat aacggtatgt taggaatgaa
    24001 acgagcgtct taatatacaa aatttataag taagtatata cctatttgtt gtggcgcaac
    24061 tttgcttgtc tgtatgacta aaaataagac aggatcactg tagaacgcta tgagagctat
    24121 tttgaggagg cgtagattct atctacgcca atatacgatc tggagggtta cagcgcgtca
    24181 gtgggctttt tacgcgctgc gtttatgctt atgacgccag cttttggacg tgggtcaaca
    24241 aggttaagct cttcttcgct gtggtattcg aggtcgaact cacgttttac atgtctgatg
    24301 tagattgcgg tgagcaggct atctttggtc agaaagtgtc cgtaggattt gcggatcact
    24361 tcttctacct tctcgatttt ctcaccaccc atacagagat gattaaaccg gctgtgtttc
    24421 cgcaacattt cgtctactgg gcctgagtaa accttgtcta ctttcccaaa acggatgatt
    24481 tttcctgtgt ctgcggatac gaggcaaatc agcttgtctg gtgataaacg atctcgccac
    24541 gtcacacctg aacggagtgt gttgaagtag ggggcatcca atccgatgat cggtttacga
    24601 aatgcgagta atggaacgta cctgatgcaa ctgtttaaat gaaaattcac accagcctta
    24661 tcaagtttaa ttctggtttc gtagatcggg cacttcgccg ctataccgca gatgtcacaa
    24721 agcaacttct gcttattcag gttggaattt gattcgatag tgtaggagcc gtcttcaaga
    24781 cgacgaaccc agcgcgtgcg ttttagatcc atatgtcatt tttctgtgat tgttagccga
    24841 ggcaacgata acccaccagg tgcgccgaag gtagtaaatg cctgttttaa ttattcacat
    24901 atccacagga tagatcctaa taaagagatc catagataga tccttatata gatcaaataa
    24961 gatccccgat cgctgtaagc cgcgccacga ctggtctgaa gtcatgttca tgtatgctgt
    25021 cagcggtaat tgatagtctg tcagcggttc accgtatgct gccaactgtt tttggtattc
    25081 tgtcagcggc aaatcaagta tgctgtcagc ggttgaatga caaaggtgtc cacatgtcca
    25141 caaaaaataa aaaaggtaaa aataacaaag aagtagaaga taacatcgac aactttgaag
    25201 aaggttccct tgaattgtac acaggggaac ttgtccctaa tagcaacaat acagtccaac
    25261 caattgcgtt gatgcgactg gggttgttcg tccccacgct taagggaaca aaatacagta
    25321 aacgtaacaa gccaaatgag atcgatgctt caaaggagct tgtccagctc gaagtagccc
    25381 gttctgaagg ctattccgat atcaagataa ccggtcctcg tctcgacatg gatcatgatt
    25441 tcaaaacgtg ggtgggcgtt gtgcgttcgc tggcagaata cggcgaacct aacggtcgtg
    25501 tcgagttaag catcacgaaa tttgctaagt tttgtggcta tccatcgtca cagatccgca
    25561 aaacactccg cgaccggctt acaaacagtc ttttgaagat catgcgtaca actctgtctt
    25621 tccagagaac gtatgaagaa aagaacgtcg acggctctaa caagatctcg cttctgatgg
    25681 tgcacctcat caatagcgtg gactacaacg agcagaaaga tacggtggtg ttctatgcag
    25741 aaccgaagct ggccgaacta tatcgctttg accataaggt tcttttgcag ttaaaggtta
    25801 tcaataagct accacgcaaa gaaacagcac aggctctgta caccttcatc gaaagtcttc
    25861 caaccaagcc tgcgccggta tcgcttgctc gattgcgtgc gcgactcaat ttgagtagca
    25921 ggaatgtcag ctcgcagaat cagactatac gtaacggctt aaaagctctc caagatttgg
    25981 gctatctcga atacagcgag attaagcgtg ggcggtcgat ctatatccag attcacagcc
    26041 gcaatccaaa actcaaagtc gcaccgccaa aacctgaaga catcgaaccc aaaaaaccag
    26101 atgagaaagc tggggaaatt gatgccaaac agaacattat caacaagata accgaacttt
    26161 cgcagaattt gacgcctgaa aatatcaaga tgattgagat cctttccaat agtctcaaac
    26221 tgctttgata tgctgtcagc ggtgaaatgt atgctatcag cggctatttt tctgaaaagt
    26281 atgctgtgaa cggttaaaga tatgctacca gcggtatagt gagatgaacg tatgctgtga
    26341 gcggtaaatc atctatcacc actcaatgct ctgtcatacc tccccagtgg ttactaacct
    26401 cgaagagtgc attccatata ggtttgcgct cactaacatt ctattgctat tgcgctgatt
    26461 gcttgtagtg aaaggcattt attgcctgct actccctgat agaacgtatg ctgtcagcgg
    26521 tgttttattg aggaattttt gtttggatat gctatcagcg gtaattaatg gcatatgtca
    26581 gacattcgta tgctgccagc ggtaattttg ttctggttat acccataccg gtacacatta
    26641 ttcatgcaat gtcttaagta tgctgtcagc ggtaaaaaat cgagtaaagt atgctgtgag
    26701 cggtgagata gactctcgat atgctatcag cggtagttga ttgtgtatgc tgtcagcggt
    26761 gacgactatg aaatatgact gcggaacgtg ccgcagtcag tttgcattta gtgtattttc
    26821 tttaacagac cccaaagtgt accggctttt gtagtcagtt ttcctgtttc tggatcgtac
    26881 atacgccatt cacgccgctg gtggacgata tacccatctt cacgttccag acgctcaaga
    26941 atgccaggct gcttgaaacc cttagcacgc cagtagccgc ttgttttctc gatttccaga
    27001 cctgccagtg ttaatgccat tacccaacct ccttgcagtc atcgaaaagg tagctttgct
    27061 gagaagcgtg aacgccatag aagcccttgt atttgttgta tacgaagtgg tcttctccga
    27121 cgccggttaa tttgccatta cgatttgtga gatatgggga tgacaacact cgatcgtcac
    27181 gcacaacata gaactggtcg ccgctgtcta cgaccatcgc tccatatggt gcttttatga
    27241 cgtcggtaag agcgccattc ttaacgtcgg ctacagacat ttcacactga taaacagtgg
    27301 tgtcggccac aacgatcgaa gaaagaaaca acagcgcgaa tgggatggtc ttcatgctgc
    27361 cacctctttg attacatcaa atgctgaatt aaatacgagc acgatagcca aagcgtttat
    27421 gactacgtcg tgcaccgggg aaaagacttt acgaatgaca ccagtgaaaa tgcagtcgat
    27481 gacaaaagct attgaaacga tgagtaacaa gtattcaata aaaatcttca tgataggtag
    27541 atactaactt atataaactt tactgtaaac tcactcaaaa gtgctgatgc ttaaaatacg
    27601 ctcaagatgt tcagcatctt cattactgat ttgttgagtc tcttctacgt accaacagtt
    27661 gccgttatgc caacaaacac catcatcatc aaccatcact gaatcagcgt catgacctgt
    27721 atattcaaca agagtacgct ggatcagcgc gtcagtttct tcatcgttaa ggccatcgac
    27781 tttgaccata aaaagcggaa acacgtcata gacagtgctg gtaacgattc gaactaaaac
    27841 tctcatgtgt ttttcctttt gtggttagtg tttacttatt ttgttagtta tgaataggcg
    27901 atcaatcttc ttggttaggg aaaaactgtt caataactct gtcaacggct gtgtctatat
    27961 ggagtagatg ccacacaacc atatctttgc ttcccatgcc ttgtgtgaat atcacgtcat
    28021 acttacgcca tgcgcagtgg aaccaatatg ctgagtcatc aattggtaaa tcctctcccg
    28081 cctctttgaa gtatttcagc agcattgcct gctctgccgt tggcgttgga cctatttcct
    28141 gagcgtacat acggatgaat cgctgccagt ctgtctcgtc gggggttgga tctggtgggg
    28201 tcatttctgc gatagcgttt cgtagctgat gcgcccaatc aggttcagga aagccttttg
    28261 ctacttcatc atcaataaca tccagagcca cgtttgcggc atcgtagagt ttcttaattg
    28321 ctgccatgtt tacttctcct tataaatagg aaaataatca cctttgaaaa aataaagcaa
    28381 agatatattc actccgatac aagacgttta tatgcttcga tgtctcgacg tgcttcaccg
    28441 agttttcttc ttaggtccat attttctgat tcaagttttt caatgttttt ttggtatcgg
    28501 ccacgatgtt cttcccaggc atcctgatat gccttcatat atgtcgtatt agcctttctt
    28561 tttgcctgac gaaccgcgtg gtgattttct acaaaccagt cagggtcgtt aaatgctgct
    28621 cttgcgcatt tataccaata atcggttgac tctctgttta accaatgaat ggtgataaat
    28681 ggtaatcgaa tagacagaat ttttcgtttg gaatctcgct caccaaacat atgccctttt
    28741 tttatcttta aaccgaaccc aggttgaatt aaaagcattg tcagtacctc agatgcattt
    28801 atggcagtaa ttctttaccg ctttccattt tggtgataga gacagtagga tcatcgttaa
    28861 tgcatgattc tgtgcctaat gctgctaacc ctttacgcat aaccgtgccc gcaacatacg
    28921 cctcaatctc cgcctttcca gatgagacaa tttcatccat ttgttcctgc attaattcgg
    28981 tagcaaagca cagattttca ggaaggttag ctacagcaga tgctagtttt ctatatagtt
    29041 cttcaaggtc acgttttcca gctcttcctt ttttcacaag ttcacctagc tctgaaactt
    29101 gctgattaag tcgatcaatc tcactattcg tagcggcctt tatttgatcg ttgaattttt
    29161 gccgaatact tttttgctcg gcaatatcat gaactctaat tgagtctcca atggttttat
    29221 atgaaatagt gcatggcaca ccttcgccca ctccaaaact gctaaccata gcagcccatt
    29281 gtgcctggct aagtctaatt tcaactaaag gccgtctatt gcaacttctt tgtgggcggg
    29341 ttcgttcatt gtctaaggac agttccgcct cgtaaacgcc tatttcaata tagtctctat
    29401 gttccagttc tgaatcaaat aggcgaatgc cagttgtgct aacacggctt gttttcacaa
    29461 gaccgaacgc tggatgtgtt tgaatttttt ccccatgttt attaagggtc gtttttggtt
    29521 cttcaatttt tcttttcatc ttattaacct ctatgcttca tgcgtaaagc taaatgacaa
    29581 ttttttttaa tatccggcgt attcgtattc ttcatttaga ccgtattctt gtggagtgat
    29641 accaggacaa cataactcaa aggcgaactt gcgccatttg attttttctt ctgacgattg
    29701 tttgttattc gccggtgcaa tgtagtcata ttcccatttg ccgttgcgaa gtaaaatgaa
    29761 tccgcagtta ttgccgaagt cttcgtctgc ataacgtatt acaaactcaa ggtgcgggaa
    29821 ctttctagcc atcgcttgat aaattggttt tggcatagac caggctgttt caaagcgaat
    29881 caccaactca ccaccagttg cggcatgtct tgcaagacgt tttttataga ttcgttttgc
    29941 atatgctcga acgtgtgtat ggcggaaatt atggccgtac ttaatacgtg tagggatacg
    30001 ttttacgggc atttcgatgt tgtaggcatt ccattttgtt ccccaattag ctcgcgacca
    30061 gtcaaaccat gagtaatagc catagcgacg ataattctca agccgcataa gagcctgctc
    30121 tttgattttg cgaatttcct tgcgtgtcat tcctgattca cgcatagcta attcgacctg
    30181 ttctggagtc tttaattcac cgaacatgtg gtcgtaaact gtttcgcccg ccattgcaga
    30241 ggccatgcgc ctgatatgat tgttttcttc catttctata cttttcggac gtctgcaaat
    30301 ggtattaaag tcaattagac cttttttgtt agttatggca cgtatgaaat ctaagcgttg
    30361 tttatttgta ccacctatga ctcggatttc gtttgtaatg tggttgggca taattaactt
    30421 aaccttttaa caaattgttt tcttgtgtgt gataataacg agaataaaac ggcgtccaac
    30481 tggacgcctg atcttcttaa tgagagttat ttcactgtaa tgaaaggagt gttagcccct
    30541 tgagtcatgt actgcggtaa ctggccattc catttattga tggcttccag ttccataacg
    30601 ttcgggtttt gacgcagagc ttcaccgcgc agacgaatgg cgtctgcttc tgcttgcgca
    30661 cgagcacgga tagcatcagc ttcaccatta gcctgttcac gtagcatatt cgcctcagct
    30721 ttacgttgtt caacttcctg ctgacgttgc agagtacgct ggttagccgt tactttggcg
    30781 ttgatagatt caatgacggt ttttgggtaa tcaggctttc caacccatga caggctcagt
    30841 acctcaatac caaccggaga catctctttc tgaatatctt tcagtgcgtt gtccagcaat
    30901 tgcgccttac caccgtcgat aaatgagtca gtggtcatac ggctggccaa acggtttaga
    30961 gagtcggcaa ttttctgacg cagatctgat tcggtgatat cgtctacgcc tttgcgatag
    31021 gtctggaaca ccgtcgttac cttgtcacga ttaaccagat acgcaacgcc aattttgtga
    31081 ccaatagcag taccgtcact catctggaat gtgaacggtt cgtcgtaggt cttcatttgt
    31141 ttgaaggtcg ggaatacgta aagttcggtg ttaagacctg tccattggcg accaacgcca
    31201 accacttcac cgatcccttt gtcttcacct aatttgttta ctttgatgcc aacgtatcca
    31261 ggctccacgc gatcacagcc agtaagaccc attgtgcaaa tagccgccag agcaactgca
    31321 agtaaacctt tcttcattac ttatttcccc tttgctttga ttaatgagtt gataaactta
    31381 cgaccagcaa taagaccgat tacaggagca ccaaaaccaa cgataactcc aaacatgacg
    31441 gctaaatcac tcttggtaga aatcagcgac ggaaccagta aaccgtagat gagagccact
    31501 gaaacgcctg tggctactgc caatagatag attttgatca tgtgttttcc tatgtggttg
    31561 ttatgcgaaa ataataagta agtacttata tattttcaag agataaaaaa ggcgtcctgt
    31621 ggacgccttg tgtttactct tctactgact cggtggggtt gtcttttgct cttcggtcgt
    31681 cgattgcctg caaagccgca atgatttcag ccagtggctt ctcccgatac atctcgacga
    31741 tctgcgattt ggtgtatttc ttgtcaccaa tttcaactcg cccgctggcg ttctttggca
    31801 ggtatccttc ttccagcatg tactcgacga gcgattcgat aacgtcgagg ccacgagtag
    31861 gatcgaagta gaatttccag gtgcatttgc catacggtgg tgcaactttg tttttgatgc
    31921 attctgcgcc tacgtcctgg ccgatcttct ctttaccatc cttcatcacc gatgcaccaa
    31981 gacggatacg tacagaagcg tagaacttcg gtgaatctcc acctggagac gtagtagggt
    32041 cgccaaacat tacaccgatt tttgtgcgaa cctgattcaa gaagatgatg caggcgttgt
    32101 atttgcgtgc ccacaaagcc agagtaggga agttcgcact tgtcgcacga gccagtgccg
    32161 tattgtcgtt catgtttagc tgatctttgt ctttggcagt gccttcagcc atcttttcga
    32221 acttctcggc tttggagttc ggaaccatag acgcaagtga gtcagccacg atacagatag
    32281 gtgctgattc aggaataagc tcttcatcac gtaccaattt aagaattgtg ccgatcaact
    32341 ctacagagtc ttcgaaggta tcaggctgct tgtataccca ctgaccgtca tcctcatctg
    32401 cattcaaacc attggctaca gccaggccaa cgtcaaaact gttctcatga tcgaggaata
    32461 ccgccagacc atcttgtttc tgtgcggaga tcattgctgc cgttgccaaa aaggttttcc
    32521 ccgcgcttgg tggcccgaat acttctacga tacgaccaca tgggaaaccg ccatcgtaac
    32581 gaccggaaat cgctttgttt aacggaggaa agcctgtgtc aatccaatga gttactttct
    32641 gaatttcgtc gttgctaccg attttctttt tcagagcaag tgccagtgcg gattttcctt
    32701 ttgccatgat caggctcctt ttgtttcgtt gattcgtttt aaagcggcgg attcgtcgaa
    32761 tacaatcgca tcgtggttaa ggagtctgga tacacgagcg aggattttta cgacctgctc
    32821 gctgactaat ccaaactcgc gatctgtcgc tttcattccg gcagcgccta gaattgacgg
    32881 cagtgcgatg acagcgtact caccgtgata aaagacaatc tcttttgcca gttgggtagg
    32941 ggtggttgta gcgccattaa taatcgattt aagcattagc aatacctttc gaatggaaga
    33001 acaaacactt caaggtcttc aaggaacgaa cggaaattca gctcgtagca cagttgctcg
    33061 aatgctttca cgtcacgatt gcctttgatt gtttcgattt cggtaggcgg aaacttcgtc
    33121 tcaatcaggt tcattagcgt gatgtttctc ttgaacgctt cgagcattcg acagcctgtt
    33181 ttctcgttga aggcattctt cgctagtttg ttgaaggcgg tcttatggcg tcctttgtca
    33241 accacgattg agccgtcgtt gatgccgcgt accatcgttg cgacacttcc ccattcatgc
    33301 agcagctctt tcgcaccgcc agcaccaatg ccaccaacac cgctaatgtt gtccgattta
    33361 tcgccttgta atgcttttgc ttccaaaaat gcgcgaggag tagcgaatcc tgtcagctcc
    33421 gcaaattgct caaaattaac ctgcttgttt ttggcgtctt cgcgcaggct tacccagctt
    33481 acgttttcgc gaactaactg aagccagtcg ctatcgcctg ttaacagata gatgtgttca
    33541 acaattggct gcggtgcaat gctggctacc agcagcccgg ccagatcatc cgcttctgca
    33601 tcttttgcaa tgagttgggt aactccaagc gcggtcatca ttttgaggat gtatggcttc
    33661 tggatagcaa agccttcctt catctttttc atatcaggat cgtcgtcgcg atttgctttg
    33721 tagtccgggt aaaagtcgcg acgcttgtca ctaaatccat cccaaagaat cataggtcgg
    33781 gcatggagga tggaggcata acgacggacg ttcttaacaa agccgaaagc agcctgtact
    33841 tccatttcgc cgttgtgcaa tttgtcagat tgttggtggt aataacccag gctattgcca
    33901 tctacgaaga gataattcac cggtacattc cttccaaaaa gtaaggcgtc cgtagacgcc
    33961 ttactggtca cgtaatggga ttacagagag tccagctctc tcagtaggtc atccagacct
    34021 tcatcttccg tcgcagatgc ggctgctaca gatgtcgctg cgactgcttc agactctttg
    34081 actgatacgg caacggcagc ggaacttgcc tctggtttaa attcagtttc aacggcacgg
    34141 aggatttctt catcaaccag actggttggt tcagaagccg gtgtgtgcgc ggttgctaca
    34201 gccgtcgctc cttctgtatg accagtgaca gagccaaatc caggtagtgc cgcagccgat
    34261 gttttcgcgg ttgaggaaat tgctggtgca gatgctgcgg cagttggtgc agcgatgcca
    34321 atcagacgac ccatagtgcg aactgtcgac agaagacgag tttcatcagc ctgatttgcg
    34381 tatgcgatca gatcatgctg ggtgttccag aatttatctt cgatatcgcc tttgtagact
    34441 ttacgcttag gcgagacgtc atatttggta tcgcgaccag agccagtacg tttaatcagg
    34501 aatgcgtagc cttcctcttt gctcagtgga ttgccgatat catcagcgat gtcttcggtg
    34561 attgctttgc agatatcatc gaatacagta gacggcagct cgattaattg gcatttctca
    34621 gcatctgcga aatcctcacg agcagaaaga atgccgttga ccaggtagcg aggagtggca
    34681 cgcatttgac cgatgcgttc ttccattgct ttgttaccct tgtgacgagc gcgaccttcc
    34741 atcaccattt cgcatagctg acaagcgcga ccgtgagtat gttgctcaca aatataagcg
    34801 ttggttgctt ctttaccttc ctcgttctga tacttaacgt agtgcatacc gaaagtctgg
    34861 aagaacttac cgtttgggtc gtctttattc gggaagatgc ggatatagtt gacgccgtct
    34921 tttaagcgag tcagatcaac gttgttgcca cgtttggcgg caatgtcttc acgagttttg
    34981 ttaagcaaat caagtaatgt cttagacatg tgtttctcct tgttgtgatt tggccgatgg
    35041 cgctatgcgc gttgggcttt cgttcattcg tggctctttc gagctgttaa atgatagatc
    35101 agtacttact tattatctat caaaaatcaa cggggagtaa taaagcgttc agagcctaat
    35161 cgctctaatt caacgatggc catctttgac gcctgaacga tcatgtctcg gcgatgcgaa
    35221 aaggcggcga cagcatgttt gtatatgtct gctatgtgcc gtgcgtcatc caacttctga
    35281 cgtttagata gatactgtgg gttcgtttta accttagcgt ccagtacaga ctcgttgaac
    35341 ttaatgccgt tcatactcaa gttcttacgc tctgtgtcgt atagctttgc ttcaacagct
    35401 tcaaggttaa gttttgcctc tgcgacactt ctttctgcat gagcgagttt tgagccatac
    35461 tccattaaaa ggcggggttg tctacgccag acttcctcca gattgtctcg gtcaaactcg
    35521 agatcagaca ttattttctc gtaaatatcg gtgatcatgt gctttccttt ttacttatca
    35581 tcttatattg attatatcat tgtgatgata aggcaaggag ttcgaggata ggggatatga
    35641 ggggataaga gaaaacatgg ccatagaggc catgtttatt ggatcacgac ttaatagttt
    35701 ctgctacgtc ggctaatatc gcttctagtc gttctccctc ttcaggtctg aagtaaagaa
    35761 tgttggggtt aaacccgtaa aagactgtaa cgtcaaggtc tggaagatac tccttcctac
    35821 cgactaaatc ggatggcttg tttttgttgt taaacaatgc cgttgcccga ctgccacacg
    35881 tcagcacata ggtcggacgt accagattga tctcttcacg cataaagtcg gtgaactggc
    35941 cgatctcgtc tttggtgtag tccttttctt tgtctttcac ctttttgcaa acgcctgtga
    36001 catagagatc gcccatgcgc aaatctccag ccgtcaacag cttggcctta aaatcgtcgt
    36061 atccgttctc catgaagtaa ccggttcgag catcattgcc gtttgcattg tccagaatga
    36121 tcatgatttt cggcttaata ccaatgctgg ggcggattag ttcatcacct aagcccattt
    36181 cagccgccat ccgcgtcata agtacgttaa tttcagccga acgtttggag ttcatctcaa
    36241 acggacgtga ggctttaact gcgtctatga caagatttcc catcaattcc gcttggtcgc
    36301 ggagacgttc tggatcagtt gctggcagac tgcctggctc gatggatgca aacgccccta
    36361 ctttttgcag tgattcgcgc actcgactat tacaggcacg tttctcgaca gcttcttcga
    36421 attgcgccag tgactcgaat ttgccgccaa ctttttctcg cgctctcatg atggcctggc
    36481 agccattctc agaacaacct ttcacagcag agaagggcgc atacagcacc tggctgccat
    36541 cttcaagcgt gcggatctca attcggttag atgacacgtt aacgtctggt ggcaatacgc
    36601 gaataccata ggtcagcgca tctttaacca gcccctggtg tttatcttcg ccaagaatag
    36661 tgagagcagc agcgaaaaac tctgcgggat aatgagtttt caaccacata gattgataac
    36721 tgattaagga gtaagcaaca gagtgtgatt tgttgaactg gtaggcacca ttcttttcga
    36781 atgcggtcca gatctccttc gctttcatct ctgatagccc tggatgtgac gctgtaacgc
    36841 gtactgcgtt tataggtagt tttgcgccgt actcaagtgc ctcttcgaca gttttcatag
    36901 ttccgtcttc gcatttaaaa tgttccgcac ggtgaatgcg ctgtgtagtg ccatcttcca
    36961 gctcaacgtc tatccagcca gcttgagcct gaacgatgaa cttctcgccc atgctcttca
    37021 ttttttccat gtcttttttg cctatcgctt tacgaacgcc gtcagcttca gccattgtga
    37081 agccagcaag caatcgagtc gcgttcatcg tctgttcctg atagagaatc acgccatttg
    37141 tttctgcggt aagttcatca agaacggggt gtaatgattc cggcgtcata aagcctttgg
    37201 cgacagaaac ataatcatcc aacatgcctg actgaattgg tcccggccgg aagagcgcag
    37261 tcgtggcgac aacggtttta aagctcattg gctcaatgcc accgcccaga tctttaagca
    37321 gcttgcgcat tgggcctgat tcaagctgga aaacaccttg agtgtaccca gcagcaaaac
    37381 catccagcac cttgcgatct tcaagagaaa tggtatcaag attgatatct tcacctgtgt
    37441 tctctttaat gtagcgtttt gcactatcga gcaaatcgag agttgccagg ccaagcacgt
    37501 ccagcttaat taggcccata gcctcgcagt aacgcttgtc aaatgcgata caacgcgcgt
    37561 caccacggcg ctcaaccggt gtacgttctg tcagaggaac acctgctacg atcatccctg
    37621 ccgcatgtct accaaagcca cgcataaggc tttgtaactt gcaggctgca ttgaatgcat
    37681 caggatattt tgttgcgtat ttgtcgagac ttgccagttg ttcgcgcagc tcttccaatg
    37741 gaaggctatc atcctcgacg ttcttcagtt cttttgatac cgccatatct gcggactcca
    37801 caccataaat acgagcggta tcacgtagtg cagaggctgc gcccaggtag gtgaagttcg
    37861 gaatgcctgc aacgtaatct tcgccgtagc gttcattcag atactcgatc acttcatggc
    37921 gacgtgcctg gctgaagtcc aaatccgcat ccggcaagtc gagacgctca gggttgatga
    37981 aacgttcaaa cagaagaccg tgacggattg ggtcgacgtt ggttatgcct atgcaccatg
    38041 ccaccagaga tccagcggag gaaccacgac caggcccgac aggaatgcca gtttcacgac
    38101 tatgattcat cagatcgcgc accatcagga agtaaccaca gaatcccagg cgagtcagcg
    38161 tgtccatttc gtactttagc cgctcaacat aaaccctgtt ctcagaagca ggtggtgtgt
    38221 atccaaactc ttttgtggta agacgtttac gcagccctgc aacagccagt ttcatcagcg
    38281 ttgcaggttc gtcgtctgcc atcttgggca gtgctggtgg caattcatgc cagcgccatg
    38341 tgcaggcatc gataatggta tcctgcgttg ttgaggccat tgcagctgtt accggcacat
    38401 ccatgcgaac ggagaaggct ttaagcgcct caaggagatg gcggcgacca ttgacggcgt
    38461 tatctcgctg gtgggggata cgcagacgat gcggctggtc gatttttatg ttgttcgtaa
    38521 ccatgtgcgc aatgtcttta atgtctgcat cgtcgatcga ttcgtaataa gcgggataga
    38581 acgccactgg ctctattttc aatgcgctgg cgactttcat cgcccggacg ttaatctggt
    38641 cgtagaatgg ggtagggtgc ggataaacca cactatagaa gttgtctttt ccacccggtg
    38701 taatcagtgt gcttatgatt tttgcaaaat cgttgcgttg gaacacgcta ccgatgtcgg
    38761 aagtgagcag gatgatgttg cctttggcat atgtagaaac caactgttcg agcgagagac
    38821 gcggtacaaa gtagaactgt tctcgtgtgt tggccaccgt cattagttcg cagatgtcgc
    38881 gatatccttg ctcattttta attagagcgg taaacgagta gttacgtcct cgctctaatg
    38941 attccataca tcccttagat tctttagcaa gtttatcccg gtgctcgtat gtgggatcat
    39001 caacgatgtt tagtttcaca ccacaaataa ccgccatgtc gtcaccagcg gcacgttgta
    39061 gtggaataac actcgcaata ttcatgctat ctgctgaaat tacagcggtg tagccagctt
    39121 ctctggcaat cttcaccgcg ttttctgctt ttagagccga ctccccaagt gagaagtcag
    39181 ttcgaaccat cagtgccttc atgtgttttt acctttctgg tttttttgat tttgtcattt
    39241 gggaagccta cgaacttccc atgcatcgaa atcgcaactt cttttgctga ttggtgacaa
    39301 taaggcttgt ctggacactc cagacaagcc ttaccagttt cagaagcagc gataagagag
    39361 ccgaaacatc ctttacgcac gatcaaccaa atattttttg aacaacttca cgagccgctt
    39421 gtgcagaagc tgaagggagt ttgttaatga aagaacgctc aatacctgtc acaaaatcgc
    39481 ctcgcatcat tccaatcttg gccgacaaca aaagctcacg aggcccaatc ggttggctga
    39541 tattgtgttc ttcgtatcca tcgcgaacga taccggcaaa cttaaccatc ttttcagcgt
    39601 attcatccac gatgcctgca tttgtcaaca tgttgatctc tgcgtctttg ctcatgtatt
    39661 tcacattcga aacgatgcca aaacgcgaaa agttcgcggc gttctggatg tttgtaccct
    39721 ggtacaagcc ggtttcatca ccagatccgt tcgtgttgcc agtgccaatg aaagcaaacc
    39781 gttcatgcgg agtaatgcga cgccattctg gagttgcctc tttgatgacc aacgcttcac
    39841 cttccagcac tggctgatac acgccaagaa tttgtggaaa cgcaaagtcg tattcatccg
    39901 cgaggtaaac ccagccatgc ttcattgcga gcgcaagcaa gccaggttcg aaatacgtag
    39961 agccatcacg cgccagaatt tgccccgtaa catgtgcctc ttccattgat gctgtatgtt
    40021 gggcacggat caacggacga ttgagcaaag cacatagctg tgtaggcaat gacgatttgc
    40081 ctgttcctgc atgaccccac aaatagccag gtatgccaag ttcaagcatc atgaaaatat
    40141 ctttgatcag ttcgaagtcg ccatatacgt agcctttctt cacttcggga acgaactctg
    40201 gatacggcgt attgacgttg acgctaacca tgagcggcgt cccacgtttt gttccgagtt
    40261 cttccaccgt tacgtttagc agttcgtgag ctgcgaccag atctgtcttg tactcaactg
    40321 tgcctgtgta gccagggcta ctggtcaagc cagaagattt ggccatttcg cttttttgct
    40381 cggcacgttt agcttgaatt gcatcaagtg cctttttcga tagcgttggc tcatctggaa
    40441 actgtgttgt gtacattttc agtacggtgt caggatcggc atcttttact gactcaggaa
    40501 tgccctcgca attaccattg gctacatggg acttaaaata gtgaaatgat tttccgcacc
    40561 acttgcaaac aaggtaatca gaaggatttt tttcattttg cagtgcagta gtagtcatgc
    40621 gtttttcctt tgttttctaa tgagctttca acttcttata taaatatacc atcaaatatc
    40681 gtaagtgatt acttatcatt aaggctattc taatcacttc aaaatgatac gagatagttc
    40741 agtaactaca gatgaaccaa gactatcaac gctcgttacc agggcatgat ttgagtaaaa
    40801 tctctccggt gcgtcagtca tgatgccaat tgccatcaaa tcaatgtcag tctgcgtttc
    40861 aatttgcttc gcaaccgaac gcaaatgagc gtcaaaacct ctcccaacag cccacggcgc
    40921 gccatcactc agaacaagca tgatcttcct gtcctccatg cgaccggaaa ataacgatgc
    40981 aaggctggct atgctttcac catccacgtt attaagaagt gggaatgtgt agcctacgca
    41041 ccccatacgt gagcgaactt cgcgagaact taccttttca ttccaatttt tgatgatagg
    41101 tagcattaaa gattcaaagc gagagaactc atacttgaca gccttaagtt ctcctgccgc
    41161 catatgacta ccaaatgtag tgaagccggt gataatgttg ggaacattta ttcgatcaag
    41221 ggcatcggcg atggtatatg aacttgcaag agccaattgt atcgttatac cactcattga
    41281 acctgataaa tcaatcactt gttgaacaca ggcgttaacg gctttagatt cttctttttt
    41341 acgaaacacg cgatcatcat tcattgctaa tctgtagata ctagaaccat gaatgcgccc
    41401 ccgacgctgg cccggtataa actgaactct gtttctgctt gctattgctc gctccaggtc
    41461 ttttgccagc gtcgacgaaa cacctgacga aagatgcttt tcaatatatt tgtcgaagag
    41521 ctggttgcct tcaggaacga tgcgatagcg gctgattgga tatcctccta aatcaatatc
    41581 ggagaatgtt ttaataagcc gtttgatatg ggcttctgcc tgatcaatcg agccgatgaa
    41641 gtcgtatgag cgattgtatg gcctgtattc gcttttcgag ctttctgtta actcgctttt
    41701 aatcgtttcg gatagagcat cttctgtcat gcctccgact tcatcttcta tgctatccag
    41761 ctcctctaaa gcctcttcca gactcatttt tgacggagtt ggaatatctg aagagccgtc
    41821 ttctgtcgtt tttctttttt cgtggtcagc ggttaatgat tctgacgccg cgttatcaga
    41881 agcatcagaa tctgatacct ctttatcatt ttctgtatca tcgaatattg tttctgatga
    41941 ttcagaatta tctgaatcat cactgtcgtt attatcatgt ttgtgcttac tgtgtgattc
    42001 tgattcgata gatttgtgat tatctaacgc tgagggtgtc tcatgatctt cgttgtcttc
    42061 attgttttct ccagaggcat ccgtctggtc agcgtctgaa ggatcggaag tttttcccgg
    42121 agctgactcc ttacctttgc tttcactttc agtgtctttg aggatcttag ctatggtcgc
    42181 agccaccttt acacaatcct cggtgcaaga catgttacgc acggccacat cgataccatg
    42241 ttcttttaat aatgaaattg gtttctcaat gacaggccag tattcatcca tgaaatctac
    42301 gaacggtgct tggccatccc aggctcttac aaccggacag agaaagaagt ttagaaaaag
    42361 cgcgcgctga tctttgccac aataagcaat agcctctgaa gcctttggtt taaagacttt
    42421 ttcgattata aggcgctgag ttgccatcag attacgtctc gttccgttaa agacctgccc
    42481 catttttcgc tcgatgaaca cgtcttctaa tgcattccct agtgaccggg aaggaacgcg
    42541 tcctttttct ctcattttgt tggagacacg aatatcggta aacaaaatgt gagcaacctc
    42601 atgatcgaga aatccgcgca cagcattcat caatgccggt gttgcgttat ccggaattga
    42661 tgggatgttt acgagaattg gctctccatc atcgttatag cgtacataag cgtcgtctcc
    42721 tcgttctgca acagggatgt ttttacctga aaggagagcg actactcgtt ttacactgtc
    42781 acggaagtcc tgaacctctt tgatggaacg ttttttagac atggctaatc ctttgttatg
    42841 aaaacaaatt atttactagt gtgtttaatg tagcactgcg cgaacaggga actaagccat
    42901 tcgcgcaggt agcagagggg ttaacaggtt ctgacggcta aagagccaga gccggtattg
    42961 ataagcgtga agcgtttgtt attaagtagg aatataaagg ctgttgcgtc atgcatacca
    43021 acctgaatct gctcatttgg taattcggtg agaatgtcag ctacacattc atcagctaat
    43081 ttttcaacgt gtccgatctc aagagtgatt aggctggaaa tagttgtttt cattcaatca
    43141 atccattcta acttaaaata tggtagggag tactattaaa tcttatatca taattgaact
    43201 tatctatatc atatctgagt tagttttgtt acatgttatt agtcgttttt ctccattaat
    43261 gaatgctgat tggttaatat ttgtgcctgt agaaaaaatg attatatctt tacaacccta
    43321 acctttgatg taatatcggt aagcacttac cagagagttt aacgcgcaaa ggtcgcaata
    43381 atgtctgaca aaaacattga atttatagaa agtcgctatg ctgcttttat cgcagggctg
    43441 attgaatcct cacctatgag tcaggcccaa atagccagac ttattggtta taaaaatgcc
    43501 aacaaccttt ctttaattaa aagtggtaaa attcctttgc ctatcgataa ggttcgtccg
    43561 ctggcgttgg cgctgggtat tgagccaagt cgtcttatga tgatggtgct ggaagaacgc
    43621 caacctgaac tcgcagcatt tttatacaaa gaaggtaccg ctcctcttaa cgaggacgaa
    43681 aaacaggttc ttgctgcata taacgagcga ttcggtaaag agaaaggcgc atcacaaaag
    43741 gttgttgagg ccataaagtc tctatgaaaa atttagacga ataagctctg ttgatagacg
    43801 atctcccttg aatttgtggt caatttcgtc taaatccggt tgctctacga ttgatgcgat
    43861 gtacgttgag aaactttcta aggcgtctcg catctcgtcc atataatcgt gccggtcgta
    43921 gacccgatct atcccctcaa gactgtggtt cattattttg cgtgatacct cctggcttat
    43981 gcctaatgct gggaagtaac tacgcgcagt acggcgcaaa tctcggggtg taaatggcgc
    44041 aagctccatc agttctggtc gttccagaat acgacgtaat gcctgggcta ttgccacttt
    44101 agacatagga aggtctttcc cgattttttt attcgaaggc acgagccact ggctgtcttt
    44161 accgtattcg aacaactctt caatgcatgt gcgcattaat gtgcttaaag gtagcgaatg
    44221 ctcacgtgct gacttgtttc tcttgccttg attccaaact ccacgcttaa gattgaactc
    44281 acttttttta gccctcagta cttcgtcagg tcgtcttgct gatacaagac atagcctggc
    44341 cgcccatttt gtaccagcgc acacattgaa gtagtcccat atattccaga acacccatac
    44401 ctctgcgtcg gtcagcttcc gctcgcgagg tgtgggcttt gcgccaccgg caactttgtt
    44461 tagtgacata tcgtttaacg gtgacacgtc tatcatcccc tggaaggcgc accagctaag
    44521 gaactgcttc atcagagaga aaacgcggcg gcccataaca attttgccat ccaatattag
    44581 agggttgacc agttggttaa ccatgaacct attaatgtca cttactttta catcagcaat
    44641 gtgcggcaaa acatgtatca aaatacaatg aacagcgatc tctggccgac gtctggttat
    44701 cagcagagat aagcgagtga ataacatgaa tgcgtccgag aatatcatgt tattgttgat
    44761 ctgtgagatc ataatggcct gcatttgaga tgctctttcg agatactcaa ttgcctcttt
    44821 tgaggtgttt tctgctgcgc gtgctctgtc aaagctattt ttcatttatt ggcattcacc
    44881 tagttacgct gatcacctgt ataagtaacc agtatattag gcattgattc ttttggaatc
    44941 aagctaaaat ctttcatgcg gaaaatgatt ccatacctaa agagtatgga atcatttttt
    45001 tctgtagagc tagttttaaa tatttgattc gatatgaagc caggcaattt catgcctggc
    45061 tgttactttg tgttaggttg ggttgttgat gctaatggta agtgtttcga tagcaagatc
    45121 atgagtttgg ttgtttgtta tatctgttaa aacaaaagcc tcatcaccaa gagcaatatt
    45181 gtctttaacc agcttgaatt tactccccgc cttgccatcg agaacgggaa ttacagttcc
    45241 tgtgcctttc tcatattgca actcaagagt gtgccaattg ttgtcgtagt caccatatga
    45301 gtctatctga acatttcgat tattgcctgt cctgtgcatc ataatgttca acttgcctcc
    45361 aattgtttga aggaagaagt tagccagaaa caggctccca gtaccgtcga atgatatatc
    45421 gtcagcaaca ggtttactgg ttttgtaata tataccaaag ccaaacttag ctttttttgg
    45481 atcagggttt ccagataatt taaacttaca aagtaattta cctccattct caagaagagc
    45541 ctgtgctatg tcagtagctt tactgatcgt ccaagtttcg ttggaaaccg ctttagttaa
    45601 agcaattgct tttccgtttt caactgactg aatatttgca ccagttacag tccatccttg
    45661 ttcagtagcg acatcaccac cagctccagt taaggaaata accgttcgcg gggttggagt
    45721 tgtgacaatg ttgtcatttt taggttgagt aggttgttct tgtttaataa tctcacctgt
    45781 aacgccattc acaaaagcag atgatttcgc tgaatgtgca agcagcgcag ttgcgagacg
    45841 atcagatatt attccgcgtc gctgccatga accaaaatga ctctctctac tctcggaagt
    45901 ccatgtttta ttatccttgc gatgaccgga accataatag ccaatagatg tgatgtctgg
    45961 atcatctgta gggctattgg ttaatacgtt attatcattc tcatctttca tgaatggaac
    46021 gaaataaatt gcttgtgagg tttttccctt ataaccacca taaacaattt cgtactgttt
    46081 tggatatttg tttttccacc agtgggtcgt atcgccacaa atccagggta catttgcagc
    46141 acttccgaaa acacattgtg cgtcgtattg agatagctct tttctgaaac cttcgaccat
    46201 cttgaggaac agttcgttat gctgcgagta cttgccagtt gccaaatcag cttcgccttg
    46261 catccagaca acaccaagaa atacattctt tttgttttta cttaaggccg cttttgttct
    46321 ggctacaata tccttataca aaggagtatt taatccccat tttaaagatg attcgattgc
    46381 acctgactct tcataatatt ggccttcaga gcctgtaaca gtgaaaccag aaccacctct
    46441 tccgcaagga acgagcaaaa tacctgcgtt tgctggaatg aatggtaata gttttttgcc
    46501 aatgtgtagt ccctgaccaa cacatccata ttcagagtcg tttgcttttg gatgcttgaa
    46561 tttagtcatg tcttgtacat catgaagaca gtgatcagcg ggaattattt cattgtactt
    46621 acaggcaaaa ccacctggtt taatattgct gcgtcgagct aactgtttta cgcgaggatg
    46681 tggccgatca aaagtttttg gcataggcag tccttcgccg tatgacattg agttggattg
    46741 acctgcgata gtaactacat aataatagtc aggctcttca tcttcatttg ataccggtgt
    46801 gggcaattgt gataaatcac cagatcgcac aattgcttga ttgtacgcat ttggtccgcc
    46861 cttacttggt tcaacaatgt ttggtgactc gaaaggttgc caaccctctg cgattttctc
    46921 cgcaagtttt tctctaagct ctttgcttga tgatgctgac aaaatatcat agtcaatgaa
    46981 tgacataatc gttactcctt taacgataaa ctttagctaa aatcgtatcg attttagcag
    47041 ttaaaaaacg acaataaaat ataagagtaa tgtatgaaag aacaataaaa attgcgtaaa
    47101 tggatatgtg taaaatatgt ggaatttcac acacagcgtt gagcggtctg gtttatgtta
    47161 gcggagtctt aatcgagttg attaaaaggc caaaccgctc aacgctgtgt ctggcggaga
    47221 gtaatggaat cgaaccatca tcgcttgcgc aatgggacgg ttttcaagac cgcttgagca
    47281 ccatgctccc tactctccag tcgttgtggt taccggtact gatctccggc ttagcgaccc
    47341 cggctactga ctgcattgca gtgtgtgaac tcgcctcgtg tgtcgctggg gcatttcttt
    47401 ccgcgcatca gtctgcgcat caaccacaac ggaaagagca ttcacgtctc gcgtgccacc
    47461 acgaatggca actcagagag tcgaactctg aaaatgttct tacctgttgt gcgctccgtt
    47521 tcgtggagct aacggcgggt gaacaggccg caccaggttg gacatatttc agcgttatgc
    47581 tgttgcaaga gagccaaatt gtgatggtcg gcgctgatct ccgacacagg gttgtagcaa
    47641 gccccgcaaa gcgcgcacta ctgtagttgc ggcacatcag cctgtgcatt caccacaatg
    47701 ttgagaacac tggttgtcac gcggcaacgc aacatttatt catagattgg gatatgaccc
    47761 cgttacgcca atgttctcaa tattatgttg gtgccgatta acgggatcga accgatgacc
    47821 attcgcttac aaagcgactg ctctaccaac tgagctaaat cggcaatttg gtggggagtg
    47881 atagagtcga accacccgag tcgcaatgac agtagattta cagtctaccc cgctacctct
    47941 acggactaac tcccctaaat tggcgatggt gggtggattc gaaccaccga ccaattggtt
    48001 aacagccaac tgctctaccg ctgagctaca ccatcaattc ggcactgcca gtatttatta
    48061 ctcaacagtg ccaatcgcgg cggtgttagt ttctgccagg aaacgcaccg ctacttgcac
    48121 ttttcgttag tgtcagacgc tttcgacttc tctcaataga aggcaaaatc tttaagtaga
    48181 tgatgtttct gaggacagca cctactttgt aatttataca atgtgatgta tggaatcatt
    48241 tgttgtggaa aagtggttaa acctacacaa caatcctgtc ttcacaacgt tgaggccact
    48301 actccgattg aatgtttgcc caacatgtta catatcaacg ctcgccggta tctctgtgaa
    48361 tagaacctta gaattgataa aaatgtaatg gcctcaacgt tgtgtgctgg ctaaccatac
    48421 cagccgggct acgtcgccgc tttttaaccc agtataaacg acataattga acaaaatgac
    48481 gtaacagaat gggcggtcag tggctaagaa tccgggagtc atatggagtt gaaaatatac
    48541 cgaccgccca ttctgttact tcatcggagg gaacatgagt gttccctcct gcgttctgca
    48601 atcacactcg ctcagtgtgt cccatttcgg taacgaggct ggaaactgac ctcgctggtg
    48661 tttggcttat taggctactg ccagatagac ttcttcgttt gcacttgtat ttaagttcaa
    48721 acagtcgcat ctcaacgaaa acaaagtcat cttatacaca aaggataagt aagtaaatac
    48781 ttatcgatat gtttttattc ggttgtgact tttttgatca aggccactct acgactaggt
    48841 gttcttgtga tggtggcagt gaatcgtttc gcttggatag taatggtttc gttctcctta
    48901 agttgcccgt agcgagtttc caccagggtg ccaagacgcc acagaccatc atcgatacgt
    48961 ttatgactag caaatttaat cagcaacagt tttacgacta actgacctac gtaaaaggcg
    49021 aatgcgactc cgaccgctaa gaaataggtg gccaaccacc agtcccaaga agttaaattg
    49081 ctcattttct aacctttgtt tcatgaacta cccgatacac acgctttccg atgcacagtg
    49141 ttttggtttt aagttcctgc ttaattaaat cacgacagat gccgaagccg ataaggaagc
    49201 caccaataaa agctaatgag atgtatggaa tcattttaac actcctgatt ctactaattg
    49261 ctgtagcaat actttccctt tatcagttaa ctggtagttg gctgagcacc acgtttccga
    49321 aatgtttgca accaacccta aacgctctaa attagctcgc gttttaggct tccagtactg
    49381 agggaactcc ggccacttac ttatttcatg aagcgtttct ttctcccgtt tacttaatac
    49441 gatcattctt tatctccgat tcgatcttcg gtatcgcgta agcaacgcgg ccatttcagt
    49501 cgtgggtggc gtaagctgcc atcaggtgtt ttctcgtgac aatgaacctc gacaatacga
    49561 ccacggtact tctctttgtt gttccagatc tcgtccagat atttatgctt aatgccgctc
    49621 gcacgaacga tgacgccgtt ttcaagacga ataactattt ttccaagcgt gttggcaaaa
    49681 ccagagtccg ggtcgcccgg ctcaaaatcg ataatttcac catcttctga atcttcgtct
    49741 tttaacttcc accagctgcg ggtacgttta aactcgtaaa cagagttcgg atctttgccc
    49801 atctcccctt cttcgttatc gtccaggcgt ttcatgaagc gttcgatgaa gtcttcgtgg
    49861 ctatggatga tatagaacgg atgcaggtga atgtcttgtg tgtagccttc actgcgatcg
    49921 tttttaaaca acgccaccag catagccagg cgctctttca gcttcatacc tgtctttgca
    49981 tactctttgg atttagcctg tgctcgccat tccggtagga agaagtcgaa aacatgataa
    50041 atagcgccaa ttgcctgcac gttctttttg cgcaatgcag atacggactg gttaaatgaa
    50101 ccggcagttc cttcaccatc gaagaagatg tgtttgtggc ctgaaagttt gcctaattct
    50161 agcattgctg gctttaggtg atcgagagac gtgattggat taccagtacg agaaaggaag
    50221 ttaacctctt cctcgtcaac aataacctcg caaattactc gcaaaccatc gagcttaaga
    50281 ctgccaatca tcggccattt tgctttagga tttggcttga aggggtattt gtcgcctttt
    50341 tctttatacg gtgacgccag ctgcacctcg aatttcggaa tgggattttc aaaaaccttg
    50401 ttgcataggc taatcccaac acctgctttc gggtctttta agaggaaacg acggaacacg
    50461 tcctgcccat cggcgcacat tgacgccacg atagactcaa cagctgctat tgctgcgttc
    50521 cccgttagct cgcgagaggc cagcttattc agtacatcaa tggctttttc gtcacttggg
    50581 atggactcac taagcggctc tgccactttg tatttcttta ccccaaaacg gatgaaaggg
    50641 ttgagcatca atgaaaccat gctttgctcg aagtcatcca tgttggccaa tgcctctttt
    50701 tttgcattgg ttcccatagc tttcatttcg tccagcttat gcttaagtgc gatcaacttt
    50761 ttcattgttg ttttacctcc atatgttgat cgatttgctc atgtgtttct tttgttgttt
    50821 cttcaagcaa cgccgcatac acgtcagtga tcgctgtttg tgatgtggtg ctcttcttaa
    50881 ccatgctatt aatcgttaca ctgtcacgtt ttcgttttaa agttctggct tgtttattcc
    50941 gttcttcaac ctccttaatg agcgcggtca tatcttcgaa atacagagat tctcctttac
    51001 gaatctcttc aaccatcatt tttaacgcct tgcatttgcc tgctttgata gcggttgcgc
    51061 acgactggaa tgaagttcga ggaagacggt tttctttgaa ggcaagaatg gtgtgctggc
    51121 aaacggagta gctgcaatat gccgactcac cgtttatctt tacttcctca caacgaagtg
    51181 aataaccatt atttcctgaa atagagggga tttttgacaa atcagctttc acaactaccg
    51241 ccataaataa tcttgtactt acctatcgtt atttgcgtaa aaaacgacgc gcggagagat
    51301 taccaacttc cccagctaat catcttgcgc tgattgcttt ctaagcgata gggggcaaga
    51361 agctctgtga catgattggt ggcgtatgat ttagcctctt gctctatcat cggtaattcg
    51421 ttagcaatcc tgaccacctt ccaagcaaat tccgccatta cgccatcaca tgccttccca
    51481 gcatcaataa cgatatgcac cagatccaac tggcttttgc acatgtcgca aagagacgca
    51541 tactcactct ctctgattac atttacggca tcattagccc ctttattaag caagacttcc
    51601 aacagatttt tcggggtaac gaaatctgct ttcagagata atgtcttttc accacttatc
    51661 gaacgcaagt actcttcgta gttattcttc atcaaaccgc tgctgcgcat tgctagaact
    51721 gcttcttgaa tggccgcttc aatagtttcg tcgtttgaaa gacaaaaaac ggtatttgtg
    51781 tagatcactc tgccatcaag ccatgctcca acctgcactt taagactgag cgggttttct
    51841 ttgctaataa aaacaactaa tgcagaacgt cgtgcacaag aaggttcacc ccatacgtgt
    51901 aacttaacct tcagataagg tagccccggt agcgggatct cgaccaaacg agtggcgata
    51961 tgatccattg ctgtttttac cgctttttca atgatgtcta gtcgattgct ctcgctgatt
    52021 tctaaaccag ttttatcaat gatgtcgcag gctaatttct gaatttcgtc tttcatacca
    52081 actcctagct aacagatgaa agtattctca cagaaagata agtaatcatc tacttatttt
    52141 aaaggtgatt gttatgatag agccttaatg cccaaaacct tgctttgcaa ctccaactgt
    52201 ttggtatacg gttttgcccg ataataggct ttgataattt gttctggcgt tgcaccgccc
    52261 gggtcgagtc cttcctcacc caaacacgca actttgacat taagaccgat actggtcagt
    52321 cgcctggctg cggccatcgt gttgcgtata gcttgctttt cactatccca catcatgatc
    52381 acattacgta atccacgcgc cttgagcgtc aggaacgcgc ccaactgatc ttctgcatcc
    52441 tgagtggtgt tgccagatag atgcatcccg aacgttccta ttggctccac gtaatcacgt
    52501 aatgtttctt cgtcgaaaat agctcgtttg acccccataa catcgaacgc accctcacag
    52561 acaactacag tctgtttgcc gactgcatta tggccgttgt agagaaattt acctgaagct
    52621 ggaagctgca taggaaagag gtagcggcgt tctgctgtac cggtaatgtc tcgtccctga
    52681 aatgtcttca ttaccccatc cagatcgtaa accggtatca gtattcgcat atcgaatatc
    52741 tgccctttta cctgatctgt atacggatct acataagcgt gcttgccttc gacgcagtat
    52801 cgtaggtcaa aatactttgc catttcaggt gatatgtttc gttcaaccag ataatctgga
    52861 agacggccat ctataggaag ctcgtaatga cgagggagtg caactggccc ctctaactcg
    52921 actgtgcttg caagcactag ctcttctttc tttggtgccc acccctggga aagaagagcg
    52981 ttctgcacat attcctcgaa ctgtcgtcgt gatttaccgc tgtagtgctt gaggaagacc
    53041 agcttattga actgaatctc ttcgggatga tcaccagcga agcatttgcc gacgccactg
    53101 gtcagattga aatatacctt ccagttggag ctgccgcata ccggacactc cttgatattc
    53161 acttcacgac cgcgagtact cacgcctcca cgtcgataaa cgataccttc agtatccaac
    53221 cattgttcaa aatctaattc ggcaattagc tctttcagct cgctcacgat aattccactt
    53281 ttaacaggca atattgtgac caatctaaat gttgatataa cataaaggct catgtgtttt
    53341 tcttttgtgg tttggcaaaa gaaaagttgt ttcaccaatg aatcaagcgt ggaggtgttc
    53401 tccacgctta ttttttaggt aacgtctaag attcgctcaa tgaagcgcat ttgttcgagg
    53461 ttttgtttaa cgcgaatgct gatccctccc tgctggttac gtgaaccagc aaagtagaga
    53521 cgagcctctc ctttcgcttc ttcttcttcg gttttgttga tcgttattac caggtcagca
    53581 atacgtactt tttcgatgtt gtcggcagcg tgcatcattg tggcaacttc tgacgcgcca
    53641 ccttcacggt ttgtctgcga tgccgtgatc ccagcaacgt tatgcttgtc ataaagagca
    53701 cgtaaatcgg tgtagatact acgaatgttg gcgcgatcat cacgaaggtc ataactggca
    53761 cgcatcaaat ctgcgtagtc gacaacaacc atgtcaggca ccatgccgtt ggctttcatg
    53821 ctgttaagca tacggtccag atctgccggt gacatacttc ctgacggacg ctcaaccacc
    53881 cacaaactac caatcccctt cgtggctccc aactctgcca acttacgatg aacctcatcg
    53941 cgccgttcca ccagcttgga catttctgtc tccgacaatc ttgcatcaaa acggtcggat
    54001 aaaatggtgg tgtgaacctc cagcgagaga tacagaacat tgtagccagc aagcgttgcg
    54061 tttatggaaa actcacccat tgcggtcgat ttaccggatt tagcgaaccc catgaagagc
    54121 accatttcac gcttcgccca gcctttttgg tacagcaacc tatcgagcag agggagtcca
    54181 gttgtaatgc tgtttggcac atactcctct gaagcctcat attcacgcgc tttcaatcgc
    54241 tcacttgcgg aggtgtagta gtcatagatt ccggtcgctt cgttcgatcc aatctgctga
    54301 accttagcca tgattgccat cgctccctga aagtcgcctt tctctttcag ttcagcagcc
    54361 ttaatcagag catcatcaaa cgctacactt tttgcgaagg ttgatacctg gtcaaccatg
    54421 tacgaggtat cggacaattt ttctgcaaga atgcgcttaa acgcagcaac aacatcggcg
    54481 aatagttcct cacggatagt cttatcgcgt ttcgcacgct taagcatatc cagaattgca
    54541 gatgaagagg gcgcgctctt gtacatacgg taatagcccg aaaccatatt aaccaatatg
    54601 gcattggccg cattggcaaa ttggctaggc acaaccagat ctcccgcacg agtaagaaac
    54661 tcgtgatcac gacaaaaata tgccgtcagt ctgttctgga aatcttcatc aaactcttcg
    54721 gacagcccgc gtcctgtatg gcaaagttct gtcatgtgct ttcctttgtt ttttaaacaa
    54781 attgttttct agtattagtt aattagatag gggatcaata aaccgccgtg cttcttccag
    54841 ttcttctgga aagtgggcgg aaataaggcg ctctggaacg atttccatta gccagatagc
    54901 ggagaaaatt gcgcgtatgc gcttgctgcg agggatggtg cgcaaacact ccagaatcca
    54961 ctcaaaatag ctttcctgaa tcgggttgaa ctgcatgtct cccatatgct taaagctaac
    55021 aagagagtca tccagacggg ttgttgcgcg tctggctaat ttctcttcga atatctcaac
    55081 cagctctggc tgccacaaat gctgtgggcg cggcagcttg tcccacagcc gtcgtgcagc
    55141 tgcggaaaga acggtggaga taaagtagtc gtatgagcag caatagcggt cagcaaactg
    55201 gcgtgctttc catagagacg ttttattggc agtcgacaac tcctgataag gcaggcgttt
    55261 taacccggtg gtgaacggag ctgtttcaaa gtgttcgcga ccgtgcgtca gcatgatatt
    55321 tgagtactga cgtttgtatg cctccgtaaa cagacaggtg gccataagag gatgcatgtc
    55381 tcggtaatca aaccacttcg tctcgaagag ttcagcctcg tctttacagc gcgacaaacc
    55441 aatgttttca gcgacccact tgtccataac agcggtattc cactctgtca tgaagtcgta
    55501 ctggtcgttg tcgatggtat cgaaaaagat ttggctcatg tggctcacct ggtaggtagt
    55561 tacttactta tcacaatgga cggatgatag cgactggtgt cagtttttgg aagtgaaaac
    55621 ggaagggagt tgttctggtg gtgtctttta aaagacctgc ttccgtatat atttaataag
    55681 ttacttatta tttatataca gaaacaggct cctaatggat accaagaaca acataatttg
    55741 actccaactc cagatgctat gtcattgaca gatttatcaa gggagatcat gttagaatta
    55801 gtgttttagc ctcgtggata ttgtcaccat gcgcatgaat agacttccag gatatggcct
    55861 tcccgaactg gctttttggc ctcagcctaa atatgaacgg aataaatggt cgatgttctg
    55921 tctgaaactc cgtaatgatg gaacccttgc ttggtatcgg cgatatgttg atcgtggtat
    55981 gccaaaccac gctgttgatg acgactacga taactatcct gaagcaagaa aagcggcatt
    56041 agagctgaat aaaaacgtga gctttgacat tgaaaaactt cctctatcta tgtttcaaaa
    56101 gaagtcattg cgtttgaaag ttgacaaagc actcaaagcc aaatcacgtc tcatggatga
    56161 agagtatata atgctcaacg aagcaattaa ggcacacgcc aacgatccgc gagtgacatt
    56221 aaatgagtta ataattaacc cagattacga aactttgcgt caaccccttt ttgacgtgct
    56281 aagcgagatg ccttacttac attttgtttt tcttcccaaa ttccaagttt cacttcaact
    56341 cgttgctcct aacacttggg aatgttcata taacaacaaa gctaagacag cgaagatctg
    56401 ctaccaagaa cgcatagcca gaggttttgg tttatcaggt gctgcacatt ggggaaaaac
    56461 aaaagcgact attcgctcaa tgcttctgcc gcgtgctaac cggttactgc aactcgccag
    56521 cgtaaaaaga atgctggatg aagctctcag gaatgggcaa aaggtactgg ttgttggcaa
    56581 ctttgttttc tggtttgagg acaaaaatca ggtaggttgg agtgttaaag ttgctagcga
    56641 aagcgaaacc acctcaagag gcaatacgct ctggaaagaa ggaactatca tttcaaaaaa
    56701 tcacggtcga attgtcgtgc tgccgtatac aaaagaaaac ggtaaacatg taaaaggtta
    56761 taccaagaat gcccctaacg acggcaatgc aattccgaga cataaggatg agtatgttga
    56821 gttgcccttt gaaattttgg atggtgactt gatgttcagt ctgttcggtg agcttaacta
    56881 cgaatagctt tcatagaacg aaatatttct aaggctgtcc aatgcagacc agccttagag
    56941 atttatatca acctttcttc attaactcgc gtttaatttc atcggtacgc atcgtgacat
    57001 cggcagcagt gatcgcctcg ttaagcttca cgatgtcctc gatttcctgc ggtgacttct
    57061 ctgccagatg gaaaatagca gcacgaatca catcagagcg ggtgaatttc tcgaagcgag
    57121 ggatgaactt catcatctcc agcagttcga agtattcgtc ctccagtgac attgtgcggc
    57181 ttttaatttt ctctttgcca cgagtcgggc gtccctgtgg tctgactggt tggcgcaaag
    57241 gagttgtgtt cttagccggt gcatcagact ctttgcgctt tgctaggtca cccattttca
    57301 tggacattat tcttcttcct ccagactcaa cagataatct acaaattctt caaactcggc
    57361 ttccgccttt ttgtcgcgct cgctaccggt catttcaaag atagaacgac cagactcttc
    57421 cgcatcatca tagacgttgc ggttatatag attgactggc gcagactcga tgccaaacgt
    57481 ctcaacaatc tctttagccg ccagaatgcg agacacttgt gatggcaaag ccgggcactg
    57541 gttcatgacc gcgcggacct tcactttatc gtttacattg cgaacattgt cgataatagg
    57601 atcgatgtca cgtaaagatt tcaaatcacg acgcttagga cgcagcggga taatgataac
    57661 gtcagccatc agcatcgctt gtcgctgaat ttcggagtcg aagccaccag catctaccac
    57721 tacaaactca gctctaccct gaagcgattt taggtgctta atgatgtcat cctgaacgta
    57781 tgcaaaagga atcagctcaa ggtcttcgtt ctgtcgacgg tcttcacacc agctcgttgt
    57841 cgtgcgctga atatctatat cggtaatata aaccttcttc ttctttttga ctttcaggca
    57901 aacggcaatt tgctgggcaa cggtggattt gccaggcccg ccctttgtgc cgccaaccac
    57961 aaagatcttg gtcattggag agtttccttt gcgtctatta ttattgtctg aaacaacttg
    58021 ttttcttata tgtgatatag cctaaatgcc tacggctgct gtgtaaaggt ttaatgatag
    58081 gtacatagat ttggtaagga cttaataaca aaaaacccgc cgtagcgggt ttaggaatta
    58141 gtggttggta gacctggcat caaatgttgt aacgcccact tcaatgtggc ggtcttcttt
    58201 ggagatgatg gtgagaatct gactagatgg gtctatgctg acagccatag ggttccagtg
    58261 acgtaactga gcctcgctgt tcaatagcag cgcatcccac ggtttgacgc cattctgccc
    58321 aataccacct tcaacaagga catctataaa ttctttattt aaattgtcat agatttggac
    58381 acgaccgacc aaaagcatac ctacagccca aattctggca ccgtaaaccg tctgatggtc
    58441 atcacatact acatggtcat gccaattgag aagaaaattg taaactcttc tagcaagtcc
    58501 cttttgttcc tttgaagggg ttacaaatgc ccctttaatc tggaccccaa ggtgagagcc
    58561 tttttgtgag cgaaactgga atcgtttgta gacgaacctg gcgatctgaa tgccgtcatc
    58621 atcttcttgc tcatcaccaa ctaattcacg aacactatga actatctctt cttctgaatg
    58681 cccctgctcg tcaagatcca ttgcatgttc ttgaagccaa tctgcatcag ggtcgcctac
    58741 aaatgacact gtcaaacagt aatcataatt agacccgatc aaggaccact ctgtgagccg
    58801 taaaacagaa aaatcagagt taaccatgta gccaactctg attttctctc ttgcttcaca
    58861 ttcagggaaa tcagacagta cttttgaata ctctgctatg aatgatggtg acatttagtt
    58921 agcaagccgc atatccgtag atgaatccag tttcgctatt ttaacatcta cgcggtattt
    58981 atctttgata aatttagcta ttttgctttc aatctcatag aaagcatcct gttcacaacg
    59041 cacaacatcg ggaaactgtt tttctggctt aacaagaaaa cgtaaccgat tgttgagcac
    59101 catcatttct gcaaacaatt tgatggtaaa cttgccttct tcaacgtcac ttttaaggtt
    59161 aacgatgaaa cgctgatcct taagcatacc cggtttgttc atttcacgaa tttggagcga
    59221 gtcattccaa tgccgcaacg gaaccgatgc gatcatcttg gtacagtctc ttatcgtctg
    59281 aatagaacga gatgcgttaa gcgcagtatg catgtttcca ccttagccgg taataacttt
    59341 atgtcatagc ggcgaccttg tcggtttaga ggctacgagt atgcgtcgat ttattaaaac
    59401 aaggtatgat ttacttgaca tccatgcttc aaatctacct tgtgctctaa taatatgcaa
    59461 attttatgtt agaaaattcc gcttttatta tggcactttt ctgccccatc cacacaatcg
    59521 ctcccctatc atattgtgaa aaagtacttc cctttcagtc tcttcggtca tgagatcatc
    59581 acggctgatg tatagcggtg tagccgcatc gcagaaaagc acaccagagg attgaggctt
    59641 aatcacgcaa ccacttattg tgcaactcat gatgaacagt agaagcatct ttgcgccgta
    59701 actcatcaag tgtttcattt ttgacatcca ctgtgctttg aagtcgtttc ctgtcttcct
    59761 gttttgcctt ctcttccatt gctcgtcgcg ccgcatttcc gcccatcgtg taagcgccga
    59821 ccagaacgaa aagaacggca gccagagtaa tcaaagcaac ttttagcttt atcatcaggc
    59881 tacctagcat attaggccat ccctttctgg tgttttctta cctgcgacca ggcaatgaat
    59941 cctgccacaa caatagtggc aataccgaag atgatgcgta ctgtatcccc actagagata
    60001 tgaccttgtg ctttatccat agcagcggaa acctgcggca taacatcggc cagctgcgcc
    60061 agaccaatac ctgctgtaac agttgcgcct gctgtttctt tagttacagg aacagccttc
    60121 acggttttca ccggcttaac gactccagct cgacgcagac cttcctcaat aacttctgcc
    60181 gcataccagg tgttcagcgt ttttagcgga cctcgcccat tctcatggcg aatgattgcc
    60241 tcaaccaaag gtcgaaggat gtcgtaatca tgcagatcga tgatcatgtc tgcggttaca
    60301 ccaacagctt tagacacctc attaatgtag gtgtcagtgt tgttttcatt cggcggtgcc
    60361 caacgttcaa taacttcgcg aatggtatca atacttgagc cgtctttggc gcgacgttta
    60421 tcgtggtagg taattagagt cactgccaga gcacgaatac cccaaacagg gtctttaaac
    60481 gtgcaaaagc gaggttcgtc tggattcgca accagacctt gccacggtga tcctttatca
    60541 agattaccgg ggttattatt acgaatgcct ctcggagtct tcatccttgt tctcctgtta
    60601 ttgcagtcca ttttttacgc catatgcggc taatcctaaa attagtgcgg ttataataaa
    60661 cgacgtcact ttagaaataa tgcctccaaa gaagccgctt gagatcgaat ctaacctatt
    60721 aagtagttta tccagattgg aatgttgaat actgtgttga gccggagtca tgtcaccaaa
    60781 atagtttttc aactgctcat gaacctcatg aactatctcc tctctgattt cttttcctaa
    60841 tttaccaaca acttctctgg cgacaatttt agctattctc tcaacctgat catgagtgac
    60901 gcctcctacg tcattagcca tgcttcctcc atgagcaatc tattttaata tccccttaca
    60961 taattttatg taatttaaat atcaactgaa atttaaataa tatataacct tttatatttt
    61021 aactaatttt caaatcttgt gtaacccccg tccttttcat tgccgttata aaggccgaac
    61081 taggtgttcc ttgccagtaa tagtgaaaac cgttaataac actattgtaa gtaaaaactt
    61141 gtgagactcc accaactgtt actgtaatat tgcttgccct tccccctgcg atgtataaac
    61201 ccccagtggg cggtaaacct ggaacgtagt gagcttgcgt aagcacgatc cccactaaac
    61261 gacctaaacc acagctcgaa gcaccagcaa ttgtaccttg gttcttttca gccaatggcc
    61321 aatctgaacc tacagccccc cagttttcga ctttatcagg aggattccct ttatagtaag
    61381 cggttgcaat tttacagccc atagatcgtc ccaccatttg actcatcata aaaggtttac
    61441 ccaatcctaa cttagtaccc gcagcgctca tccattgttc gcccgtttcg ctcttagccg
    61501 aagaaccgac ccatcccgac gctaccccca ttttacccct ccttttgaat gcgtggggtg
    61561 agtatcaccc ccacaattta aattcagtag aaaccagaaa ctccaacagc tgtcgtttac
    61621 ttctcccgaa gttctttaat ctcatgacga agtgttttaa agccctcgac taacaatgca
    61681 attatgccgt tgtagttaag acacagacgt ttttcaccag atataacgtc tgtgtcttca
    61741 gttaccagtt cagacaatgc tttttgtgca tcctgagcaa ttaaaccaac tgacgttttc
    61801 caactatcat cagagagttg gatttcataa agataaccag taagtgcctc caaacgatct
    61861 aacgcattat ctagctttac cagattacgc ttgttacgct tatctgagcg gatctgaaca
    61921 tcattaaatg agccatttcc atttacagtc aaattacctg ttatcgtacc cccaccgata
    61981 ggcaaagcac caatttgagc ggcacttata ctaatatttt ttgttccgtc gaaacttact
    62041 ccatttatag tacgtgctgt ttgtagctta gttgctgatg ctgcgttacc agatgtattc
    62101 tgatttcctg acgtattcac acctggaaga ttaatgtttg cgctaccatc aaatgatact
    62161 ccacctattg ttcttgatgt ttttaattta gtagctgttg cagcattacc tgatgtgttc
    62221 tgatttccag ctttgttaac gcctggtaaa tctatattcg ctgtaccatc aaacgatacc
    62281 ccccctatat ttctcgctgt tttcagtttc gtagccgaaa ctgcatttcc atttgaggca
    62341 agggcgccta tctctgaagc cgttggttta ttttgagaac tgtaattacg acgccatgtt
    62401 gggctatagt tatcgccgtt gtatgtataa ataaactcac tattaagaac agcaccagat
    62461 gcacctccag atgttgaagg tgttgtaatg cgtatagtgt aattacttgt accggttgaa
    62521 aacacctcaa taacagaacc agcaagaggt ataactccac atcccgttga tgcatttcct
    62581 atataacagt tactaccata agaccagcta caacgtgtgc tccactgagt tgcatcaaat
    62641 gccccttgtg aatttaacca tgcgacaaat tgtgcagttg tccactcttg agtattcccc
    62701 gtagcaagat tagcaacata ttttgctgca cctagttgtt ttggcgtgat gcttatattc
    62761 tttgagccat caaaactgac cccatttatt tttcgtgccg ttgcaagttt tgtcgccgtt
    62821 gcagcattac ctgacgtgtt ctgatttcca gctttattga caccaggtaa gtcaatattt
    62881 gcagtgccat taaacgatac cccacctatt gttcttgccg tttgcagtct cgttgcgctc
    62941 cctgcattac ctgttgtact ctgatttcca accttgttaa cgcctggtaa gtcaatattt
    63001 gcagtgccgt caaataacat tccaccaatt gaacgaggtg ttgctaactt agtggcagag
    63061 gcagcgtttc cactagtact ctgagttcct gttgagttta cacctggtag atttatatct
    63121 gatgaaccat caaacggtac tccgccaatt tttcgtgctg cttttaattt tgttgcggta
    63181 tcggaatttc cgttaagtga gcctgcaaga ccattactaa atgtttgctt tgcactccat
    63241 gtattggctg tactcaataa aggtatttta tcgccacttg ttccattttc cttcaatcca
    63301 agatactgaa taatcgcctg tacacttgct ttagaaagaa tatctcgacc aacagaagtt
    63361 attgttgcta atccagctgt atcattccct gtaaaataag gaagtttatt agcggctgtt
    63421 gttagtccag ccagtgcagt cagcgtagca tcaagaggtt gttttgtggc caacttgttc
    63481 gtcattgttg tggcaaaatc gggatcatca cccaaagccc ttgccaactc attaagagta
    63541 tcaagcgctt caggtgatga tcctacaaga cctgagatcg cttctcttac ataagcagtt
    63601 gtggcaattt gcgtattatt agtgccttga gtcgccgttg gtgccgttgg tgtgccagta
    63661 aatgctgggc tgttgattgc tgcttttcta tcagcaagat cctttacact tttaaccgct
    63721 tttgttgttg cggccaatga ttcagaggcg ttatttgtat cgctacttaa ttgaaccagc
    63781 cctttttgtg aagttgtcgc gtcatttgca gtatattttg atttagcaag atcaaacgca
    63841 gcctttactg cttttggtgt tgctgcaaat tcttctgacg aactgctcgt ctcactgctt
    63901 aatttgacaa tacccttcat agtcaaactg gcatcatgta atgatatcgc tgacaaagct
    63961 ctttctgctc tagtagcagc tatctctgca cgctctgccg atgcaagtgc tgatgtttgg
    64021 ctttgctttg cttcttctgc tttagccgca gattcagctg atttcgatga tgcgttacta
    64081 gcactagttg aggcttcagc agactttcgg ctagcttcat ccttcgaagc tctagcagca
    64141 gacgcataag ttgaagcctg attagcggat gtcttcgcag accttgccga ctctctagcc
    64201 tgatttgcag aagttgatgc actgccagca ccagaagcag cattcatttc agataatttt
    64261 gcagcctctt tagagaccaa cgcagcatta gccgaacttg tggcttctgt tgccttagtg
    64321 gaagctgttg atgcagaaat tgcagctgcg ttcttcgcct ctaaagcact tgtttctgat
    64381 gctttagcac ttgatgcact tttatttgct gcatttttag acgctgtagc atccaacgca
    64441 ctattagatg cctgagtagc cttactgctt gctagattcg cactcttcgc cgcagccgac
    64501 gcactggccg cagcctgttt tgctttatct cctgcggaat taattgcgtt agtattattt
    64561 tcataccatt ctacgtgctt attatgctcc gcgataatct tagaaagagg ttttacatta
    64621 acattagtgc catcttctcg ctcaagagta acttcatcta cggcagttaa ccaatttcga
    64681 actgtttttg catcaccaga cattcgtgat aaaagagccg taaatctcgc actaaactgt
    64741 gtcaaatctc cttcgtaggt agtgatgatc ctacatggta tatcactttg cgtttcacca
    64801 gtatagtttt caaccaacgt taaggttgtg tcatttgtta cgcttttaat ttcatacaat
    64861 ttattatcag gtgcgacgac aatcatgcca ggcaacacac cattggccgt aacgttccat
    64921 gctgtcccag caccaaccag agtattgctg ccttgtgtaa atgtgatagt accttccctg
    64981 taccacatgt tgaatctgct cctttaatca ggtgggctat ccttgcccac cattaagtat
    65041 gtaactacct attattatcg ataaaaatat ttttttccat aatcaaagtc gaccaattct
    65101 taccctgagt acattattgt cgtcgtaaac gtcgattctc tgcccattga taactaatct
    65161 cccatttcct ccactgttac cgttaatctc aagcgttccg tttttgccaa atctccatcc
    65221 ggctcgtcct cttacgaagt ttgttgattg taagtcacct acctttgcgt ttgtgatagt
    65281 gccatctttg atataagcac cgttcatata cgcaacgctg ttttcgataa caaatggcgt
    65341 tgtgatcttc ccattaacat tgttgaccaa accaaatcga tctgcccgca ccaaaaactg
    65401 agaaagcccc gttgtatcaa tacccagcgc aatgcccgct acatacttct gcccaccact
    65461 cgttgaagtt tccatcttca atgtgtacga ggttgatact tttctattag cgtcagctat
    65521 agcagaagcc tgctgctgaa taacggcaac atttccatct acagacgctt tcagtgtatc
    65581 aattcgtttt cctagagcgt tgtctgcact tgttctggcc tttgactctg acgtaatcgc
    65641 tgcattgata taatttgttg tttgcgactg gagattagtt acccttgtcg aaagcgctgc
    65701 atcagcatca gttctggctt tcgtctcttc tgctacagca gcctttatat ctttacctgt
    65761 ttgtgtcttt agtgagttaa tctgcgacgt caatgcggta tcagcggcag ctcttgcttg
    65821 agtttcaacc gcaacagctg cacttatatc cttagcagtt tttgttttaa aacttgccaa
    65881 gtcttttgca atggcagaat cctgacttgt acgagatgtc gattctcgct ctatcgagga
    65941 ctgaagatta ttagccctcg tagtaagaga agaaatttgt ctggataatg catcgttagc
    66001 gtcagttctt gctttggttt caactgctac cgccgctgca atgtctttac ctgtttttgt
    66061 ttttaaacta ctaacttctc ttgatagtgc actatcgcct gatgctctgg cttctctttc
    66121 ctcattaata gcagctgata tgtcatcatc tacttttgcc tgcaattgat taatttgtgt
    66181 ggataacgct gaatcgccgc taactcgcgc ttgttgctca cttttaatag cagcagatat
    66241 atcgttatta acttttgttt gaagttgagt tatttgcctt gataatgctt catcttgcgt
    66301 tgctcttgtt tgttgctctt ctctaatagc ggttgaaata tcagtattta ctttagactc
    66361 cagttgtgtg atctgctttg aaagagtctc attggccgat gctcttgcag attgttcggt
    66421 ccttattgcg gcactgatat caccttcaaa tttactctgt aattgtgtca cacgattggc
    66481 taacgcatta tcaccatcag ttctggctgt cgcttcttcg agaatagatg ctcgaatgtt
    66541 gtcaccaatc tcggctcgta tctcttcaac cttagacgcc attgctgaca catcatcgac
    66601 aagaatcttt tgagtcgtgg caattttcgc gttatttacc agctgtctgt gctgatcttc
    66661 gtcctgccgc aacgcaagat cgatatttgt cttagaaatg gcttcaagat tagttgtgat
    66721 ttctgaactt actcttccta cctcagcaac cgttctccgc atttcttcta cagccgcaac
    66781 actttcatca accgatgatc tcatcgtttc tatcttttta gcattcgcta gatctgcttg
    66841 tgcacgaact ttacgctctt cggcaattaa agatgatgca ttttcaagtg ccgcatttgc
    66901 tgcttctacg acaccagcaa cagaatcacc ttgttttgat actgtattac gtagttcaat
    66961 aagtgctgca tttgaatttt caacttgtcg taatgcatca tctacttttt cccgagtgct
    67021 agaaacttcc gttctaagct cgctttgcgc attctccagt gtctcgctgc gttcagtaaa
    67081 cgatgttttg aactcatcga tactactcgt tagctcctta tttaatgcgg aaatttcacc
    67141 ttccagagat tcgacgctct gatcgatatg attattgatt tcatcaacag cttgttgaga
    67201 agccttactg tcaatttcct catttaaagc ctggccaagt tctgacgagg taattttatt
    67261 ggctaaaaat gaaagaacat ctcgcgttgt ggcctctgta ccaagatttg agtttgggcg
    67321 actcaacatg ccacgcttat ttgctgatct aacccagtaa taccaagttt cactatcccc
    67381 aagaccagaa tgtgtaaagg tggtgcttgc agactctgcg atcagtttcg ccgtatccag
    67441 attgttggtc tgggatgcgt aaacattaat gtgatcaagg tctaccgaat ctggattaac
    67501 ccaattcagt atcacattac gatagtctcc aacggccgtt aatgacgttg gtgcatccgg
    67561 cggtgtcatt gtgcccagca cctgataaac ggtactgata atctctgttt ttttaccgtt
    67621 gaatgaaacc gcatacagtt ggaagtcgta gcgtccattc tccgcgacat taacgatttc
    67681 gtattgctct tcggttacac gtgccgattg ccagttcgat acattgtttt catcagaacg
    67741 tcgccaactg atccaatact ctggagattt cccttcccat gttgcagtca gttttactga
    67801 caggttgcct gggcttgaga gataagtccc ttcggtgatt tgcaaattag acggcttgga
    67861 gtaagtcggg tccaataccg tcgtattctg cgggataagc gttgcaccat tgtcgattgc
    67921 ctcatattta gacggattgt tctcaacagc ggtgatgtca aagctacccg acgtttcccc
    67981 ctgagcgatg ttaacgatgc gaacgcgcat aggttcgaga tctggttctg tgattgtcca
    68041 tacaccgttc aaaacaggcg tttccgctga cgacagagct tttgaaaacg taacctttgt
    68101 tatgttttcg ccagtttcaa gaacatcgcg ttcaacgatt ttgccttcct gattcaatat
    68161 ccgaataaag ctgccgcttt tagctaacga cacaggcgca tcgagagtga tgctgttttt
    68221 ggtaaactcc acaattcgac cggagttacg tttacctgca cgatatttgt tctgaatcag
    68281 aacggtttca ccgggcatca gaaatgaggc gtctaaaccg gcagtaaatg taattacatc
    68341 cgactccatt ctggcggtat acaaaagcca caaaccaact cggtgagcct ggcctcggct
    68401 tgtacatcca aatgctacga cttctgtttt acgctcacca taacggcgca ttgcgtcctg
    68461 atcttcaacg tactcaatgt tttgcttata accgtcctcc ttgttgttgt aggttacgag
    68521 cgcaacggat gggcgatctt tacgcgcaga acctttatag gtaaacaatc catctttgac
    68581 gttcgagttg gtaaacatca ttactggatc tgatgggcta tcctgcatga tgttaaccat
    68641 gccaccagcc caaaacacca tgccgcggaa tgcaccggca atatcctgaa ttagtcggta
    68701 tgcatcctgt ctactggtga tctgcgtatt gattgcaaag cgtttctctt taccaccaaa
    68761 gccatcatca acctcttcgt cacaatatcg accgatctgg tacagttggc cgagatcaat
    68821 catagattcc gacacatact gaccaagccc atatcgagaa ttggtaagca agtcgaagag
    68881 aatccacgca gggtttgaag atgacaacag cttaaaagag ccatcccata cgccatcata
    68941 agtgttgcta gcctcgttgt agtttgatgg tacgcgaatt tttaatcccc gcaccagata
    69001 ggaacgagaa ggcatagagc cgccaaactg ctcagaatta accttcagtc ccaccaacgc
    69061 agagtttggg tagttcatcg gtgtatcaac gatctcaccg attgagtcca cccatgtatc
    69121 gttataaaga tactgaccgc tgttatcatc agtaatacga actactcgaa cactgtatgc
    69181 ccgacctggc ttgggtaact tcagctcata actacgataa taagcaccgg ttttcttcgc
    69241 tgttaactta ataccaacgt ttttttcacc ttctgcgacc acatctacaa atgttgaatc
    69301 tccatttgag atctggaact tatactcaac ggtagtgccg tttgtatcac ccgttttttg
    69361 gtctattctt cgcagagaag ggaacttcat gatgactcga acacgatcgg cctcatcgtt
    69421 atctattgaa accgtcacat aatgcgattt ctttagttgg atattgatcg atttaggtgt
    69481 ttcgacgaaa tcaaagccag acattggtgt ctggtcttgc gagccgtctc gaaaatccca
    69541 tgaaattccg ctaaagtttg aagagccgtc ttcatttact atgggcaaat cgtcgacaaa
    69601 aatagatctt gcgccattta ccaagccgcc aattacgcct tctccaagaa gatcgagaat
    69661 agaggccata gcacgagaat ttacggtatc gtctgcttca actggtgtgc gactagagct
    69721 tttgctactt tttttgccac cagaacctgc aattaataga ggtaactttt tcttcttgaa
    69781 ctgttccatg tccaaaaatc cttgattaca taagctggtc gatggtgata gaagaactca
    69841 cgacctgtga gccaactaaa atttcctcgc catagataag ctgtacaggg ttcccctggt
    69901 ttgttgtgtt ttgaggtcca tcaaagtaat aagagttcga gttatccgcc tgcctcacac
    69961 tttcgttagt ggcttgcgga gatatgattt gcgctatacc gcccatcatc agtgacaaac
    70021 cgagaggggc aagagcgggc atcattaccg ccgatacaac caacaaagcg gcccctacta
    70081 ccgtctgaaa ccacccaaaa gcagatccgc cgcttcctcg cggaacaggg gtaatgcgga
    70141 ttttggcaat gttgtcagac tgccccatca tctgatattc actctcgtcc acagaccact
    70201 tgtggccctg tttattggtg atctggatgt ggtatctgtc ataggttttg atattgcgct
    70261 tcatccatgc tttaaaacca ggcttattgg cctcaattaa atccagagcc tgttttgtat
    70321 tgcgcacctt tagatgccag tggcggccaa aatgtttggc catagggccg ccaagctgca
    70381 catgaactaa ctcagacacg tctcatctcc cttgagcaag tctctgtgac gcaagtgatg
    70441 cgtcgtatgt ttctgataca tcccgccgta ataagcacga caactaaggc ggtcgatctg
    70501 gtgatgaaga atcattccat cgccgatata aaccgcacag tggtcaggca tttttccgta
    70561 ttggataaag aagacgtccc cacgttgagg ttctgttccg ggcgcaagcc gtaccaatcc
    70621 ttcgtttcgg tagttctggt cgagaatgtc gttatcgccg gtgtaccacg atggaatatg
    70681 caggtgtgcg ttcgggttta gttcgacgtt aaactcacgc ttcaaatagt cccgacacaa
    70741 catccagcaa tcgaatacgc caaatacata cggtctgcac aggtatggca tttcgaaacc
    70801 atcaggtgtg atcacattca tctcgctaaa atggaaaggg gcatctccct caacattctt
    70861 gcgaatagcc agaatcatcc acggaacttc cgtcgcttcg cagcctgcac gatcggcatc
    70921 agatgcttct gctgattcat cagtatgtga atgccagatt gcgataacat cacccgcatc
    70981 ctcagccgcc ataatgtcgt caacgtgcat tacaaaagtg ttctgcgggt tctccgaaac
    71041 attccgcgct tccataaagc gatatttgtc gccattagtt ctaaccagaa agccacacgc
    71101 ttcattaggg tagcgattta tggcgcagag atagatttgc tgcataacgt cagagccaag
    71161 ctcagggatt gctttattac ccataccgcg tagctccaat aaatccacca aaatggatca
    71221 caccgtcggc aaaataattc cgacgcgcat tacaggcgtc ataacgtttt gtgcagtaat
    71281 ccgcaccaga catagacgtc tgctggttat ttttgtcgaa atatggaccg gtatagccgc
    71341 attctggccc tcggtatttc cacgggcagg tgtttttaat gatctgacga tacggcagtt
    71401 gtacccccat caaatcgaac acactggaca attcaaactc gacaacctga tgagtttcga
    71461 gagttttctg ttcgataaac cacatttcat ccgggaaatg ttggtttgga tctgctgttg
    71521 ggttgccgtc tttaaaatta acggcatcga ggaagcgagc cagcgtcatc ttgcgaataa
    71581 tgcggcagcc aacaagatcg tcgttcgcct gaacttccgc agagacggtt ccggcaaagt
    71641 tcgatacctg aatttttgga cgtggcaacg ttccctggcc agttttgtca aagcctgatg
    71701 ctttgattgg ccacggctcg tatgtcactc cttgccagac gaccggttgc atcagttcgt
    71761 ttgttccggc gtggaagaat agcttccccc ctgaagttgt gttcgacata tccagtacga
    71821 acaactcaat gagtgcagag ggagataagc tctgaatatc agctttaatt cccattgttt
    71881 catccttgaa ataagtaggc gctgacatcc tgtcagcgcc acaatgatag taaattagta
    71941 cttacttatc cagatactta agcctcaaat acttgtctga atgtagcagt taagacacag
    72001 tacccctgat atctcttgac cgtatgactg tcacatacaa ctacaatctg cttgcctctt
    72061 ggattaaccc aatagaacga ttcaacacct gatcgctcag taaggaagtc atcgattgca
    72121 ttaatttcgt tgtatgatct ggtaaaggtt aacgaccatt cttctttaat acgattaaga
    72181 ccttgagcct gtcgctgctc gtagtcatca ccaaaattaa gtaccgttac attcggtttt
    72241 acgctctttt cagattcgta atctggatac caattaaacg tttgtctttc catctcacat
    72301 ccttgtgaga ctgccccgac cggggcagtc gatagttagt tacgttgagt gtttgggttg
    72361 agtgatccgc cagggcgttt ctcttgagcg atagtctcaa gcgcgattgc tttcatccgt
    72421 tgagcggcat tgttccatat gctttctgta tcgccggatt cagttgtgct accgtcacta
    72481 tggacgttga tctcaattga tacaggagaa agaacatttc cgcctccact cataccatcg
    72541 gtactgagcg ttacagggat tgttcggcca tcaggcaatg gaacatacgc ctcattcata
    72601 gaaccttccc caaacaacgc taattgaggc gagttagcaa taccgccttt ctggtatgcc
    72661 cggagcggga tcacgccgtc ttttccgaat attcctccat ttgcaaactt cgggattgca
    72721 ggaatgcctt tagttccgtc agccacactg ctggttgcgg ttttagcaga gcttgatgtc
    72781 aagctgtcga aaccaccgcc agcccaagct gaaacaagac cagatgcaac cgttgctcca
    72841 aaactcaacc acttattacc agagccggaa gcattagctc caagcattgc aaacgcggca
    72901 gacagcgcgc cggtaacaga gctgaggttc tgcatcgaga agatggagtc cttcacggct
    72961 tttgtctcgg catctttggc ttcagtgcta tcaaatagcc ctgataccca gctaccaatc
    73021 gcatttgttg cagtgccaat tgcgctggtg gtctgctgtg ttgtttgccc taatccagtt
    73081 accgaactgg acgtctcctt cgtggcttct cctaccgact tgtcgccgtt aacagtgttg
    73141 cccattcgta ccccttgatt ggcaacggcg gaagcaaccc cggtcagcaa attaccactc
    73201 tgtgaactac cagctgcggt ggttcccatc cccaacatgt tcattagagg cagcgtgatt
    73261 tgcgacttca cgaccatatt ggtgatatct ttcaaaatgg actgagatag gctggagaaa
    73321 ctcatcttcc cgttaataac gaaatcagtc aggacatcag ttaagccact aaacaaatca
    73381 gtccaggtgc tttcgatctg ctctgccagg ttttcgtatt ccagtgccaa cttctgcgtc
    73441 gcagtccccg tctctttaat aagcgcggta ttgccagcag caatcagttg attgattttc
    73501 tttgtataaa gcgccacaac tttaggatca gacgccttat cacgaagttc tatcaacgct
    73561 ttgagattgc ggttgtaggt gtcttcgaaa tcagcaactt tctcttcacg agacggcgta
    73621 tagccagcac taataatgga atccgactcc ggtgcccagg tggagatcat ctgctcaaca
    73681 ttgcggcgat taaacatctc gcgatattca ggtgtcgcat ttttgaggtc ttcaagacgt
    73741 tttttcgcct tgtcgatcat ctcttgagtg atgaactcgt taggaaccgc attagccaaa
    73801 tctgtcagcg atttcgttgt atcgcgaaga gactgatcaa acgataccgt agccttagag
    73861 ctttcaccca tttgccccat aagctgatcg gctttatcca gagccttctg gtatccggct
    73921 gccagtttct gttgcgctgt ctgttctttc ttggccgcac gctgcgaggc attagctgat
    73981 cgttgggctg ctttctcggc ggctgcggca tcctgttcac gagctttagt cagtgcggca
    74041 atggctgcgg cacgctcttc atcgctcatt ttctccagag agctggcact ggatgctttc
    74101 tgcaaattaa gctgcgtctt gagttgttta ggcccaataa tcggcttacc ttcgaagtcc
    74161 atcatcggag tgccgtcagg caaagtacgt tgataagtcg cagaatccat ctggtttcgc
    74221 atatattgcg ccagcgcctt ctgagcagct ttatcagttg tacctaaccc aagaacagtc
    74281 ccctggtttg acattacgcc cttaccagtt ttggccgcgt tatctctctc gaactctgcc
    74341 tgtgtcagtt cctgagcaac ggcttccaaa tgctcctgat aaccacggat actgccttgc
    74401 agtttctgga tttgctcggt atttccatcc tttttggctt tttcaagctg atcattaaga
    74461 gtcgctattt gcttctcggt agcattctta cgagaagaaa atgaatcaac cagtttttgc
    74521 gcaggctcca gatagctttt gtttaccgtt tcacgtaacg gtgccaatag cttgttcttt
    74581 tcgtcatctg aaagtgaacc gtcatcattg attttctgga tcttatccag agcctcctgc
    74641 cgggctttca cgaatgttgc cgcgaaaatc tggttttccg ctcgaatttt ctcaatctga
    74701 gattcggcag cctctttagc caaacgcttt gctacagcgc cgtcaccaag agctatcgtg
    74761 ccggttatct tttgatactc ttcctgattt tttttcaggc gtgcttcgat gtcagccttc
    74821 gactctttgt gagtaataac accggcagag ttagatacgt aattaacacc ctcaccagtt
    74881 tttaatgctc gttgatcagc aagaatctgc ttttcgagct tttctgcgcg gtcggccatt
    74941 tgtgcacgtt tggccgccgt catcgcctct ggtattttcc taatctcgtc aacgaccttt
    75001 gaagtttcgc tgcggagcat ggtcatgtac gtgattaggc cagcaacagc tacagtggca
    75061 actgtaaatg ctgcccctat aggatttgct gcaatgaacg ccgttaatcc agcaaaagcg
    75121 cctttaagcc ccgtaatcgc cccacggatg gcgaaaataa gagaggggat cggagccagc
    75181 cccatacgtg ccgcacgatt gaatcgagtt actgctgtag cgccgaggtt aaatggagtc
    75241 tgtatggcgg tcgccatcgt ggcaaaggtg ctaaccatct tgctgcctgc gccaactact
    75301 cccatgatcc ctgctcgcat cagtttgaac gcaaccatcg cggccacgac cttaccgaga
    75361 ttaattacca gctcttggtt ctttgctaac cattgagcaa gctgacgcaa cccatcgatt
    75421 gccgtcgtta accccgaacc taaagaattg gcaaacgaaa tcccttcggc gctattcatg
    75481 attgaagcca gttctttcat ccccttcgat agagaatcca gatatccggc ctgaccaacc
    75541 cgatcagcaa ataacgtgaa tgacgtctga agttgcgcca gcgcacctgt gtaggtttgc
    75601 atcatgtctt tcgctgcgtt ctcattctcc gcacgcagac caacaaacat cagagacaac
    75661 gcctgttttg cctcaaccgt accgctggca acggctttag tcagttcccc catagtgatg
    75721 cctgcggcgt ctgccattgc ctgcatcgcg ttaggaacgg cttcacctaa ttgctgacgt
    75781 agctcttcca ttgacacaac gcccttaccg gacatctgct gaacggccac agccgcacgt
    75841 ttcaacagct cactatcacc accaaaacga gcaacggagt ccaccagcgc cttcagagaa
    75901 ccatcggttg gatctaagcc agcagaacga aacttcacga aggagtctgt taacgcctgc
    75961 atcgcgaacg gcgcattttg agccatgtct acgatgtact tcatatcatc agcggcagcc
    76021 tggcccgggt tggacttctc cttattcaac cctcgaagca tcacccgcat acgttccatt
    76081 tcggccgcag cttcaataat aggcttctgc caaccaaaca tgatactggt gacggttctt
    76141 gcagcatctc cgatctcacc aagcaggaaa atgttgccac gaaggccaga gaacatactg
    76201 ccttcgttat ttttaccgat atgccaagag taaccaccac gtccaccgct acccccacca
    76261 tttccgcttc ctgatgtgcg aacgcgtaca ggcttgttaa tgagttgctg acgtccgata
    76321 acttcgtcca tctgctcacg aacttttttt agtccctcgg cagcctggct tgttgtcaca
    76381 ccccaattac tgagtcgctt cgtcgtggtg ttaaggcgcg tattcatgcc actcacggac
    76441 gcagaggctt ctttgacctc cgtaccaaaa cggcttgcgc ttttgcttgc ataggtcgcc
    76501 cagtcagaga aatcatttag ctctgattgc actttacgta atgacgcggt gagtttatct
    76561 actgaagaag ttgtcgtatc gacgcgctca atcagagctt taagaccaga attgaggctg
    76621 gtgatgttgc cacgcatttt acgcgtagca tctgaagcaa gctcaaaacc gacagctaca
    76681 tcctgtagtt tatctgccgt agaatcgagc ttgctttcca gaacgccaat gatacgggcg
    76741 accgaaccca aagagcgttc aaaggtttgg attttttgag caggctttgt tacctgctca
    76801 ccaaatcgag taagcagttt tcccgcacga tcgattgacg ctgtaaactg tttgtcttcc
    76861 agcgacagga taaactctac gttttgtgac attcccttgt catcctctgc caaatatttg
    76921 catcagttgc tctttggcgt cagggtctgc cctatcctgg cttggatcgt agactttatc
    76981 tgttacgact ggtcttccaa tcctgagttg caaaccctcc atgaacgcct tcacgccctc
    77041 gccatctgcc tgagcagcgc gagcgacttg caggttgcgg acatcctctt ccgcacgtag
    77101 acggtcgatg ttgcgactga gcatccagaa catcgtgaga gggatgttca gtagttctaa
    77161 cggcgacacg gcgtagtgag caactatacg actgaaatag aatccgagat ctattgagac
    77221 ggtccttgtc ccggattcat cgcgggaaat tactttgccc cttcgccagc cgctttttcg
    77281 ttttcttcat caatcacttc catagcgaag gtgaagatct gctgaagttg cggaacagtc
    77341 agtttttcaa gaacttcgtc gggcactgaa gggataacct tgcgaaccag atctgcataa
    77401 gctgtcactt gctcaacagg agacatattg gtcagatctt taccttccat ctgcttaatg
    77461 gaaacgaaca gacctaccgt catttcaacg atgggatatt cctggccgcc aaatttgaca
    77521 ctttttttag gaggtaggat ggagtcgaga tcgagtaatt ttgtcatttg ttaaaatcct
    77581 tttaaaagag aggcccatca tgagcctcaa tttatagatt aataagttgc ttgattattg
    77641 attaaccgtc actaatttgg ttactttctt gctaccgcta ttgctggtga atgatatgtt
    77701 tgcagaacct tgttccacac cacgcacaag accagtgtga tcaacagtgg cttttttatt
    77761 atcagatgac catacgcctg ttttatcaac tacatcatct ggggtgaaat tagcagtcag
    77821 ttgaactgtc gccccgacct ttacactcgg agattcaggt gtaatcgtca cgtttttcac
    77881 cggttttggg ccactcattt tgccaagaac cccctcatca tcagggtacg cgttgaactg
    77941 aacagagaac acacgtacat catcagactg gtaggacata gtgaagttgc cagcggttgc
    78001 cgctttcggg agcgtcaaca catagtcggt ggtgtcctgc ggtgtcagga cgagttcctt
    78061 cgccacgttg atcagattta cgccctgtgc agatgtgatg gtgacggagt tgtcatcttc
    78121 gctcaaagta gaaccaggca taagatcgac catgtttttg agcacagact cggccagagg
    78181 tgcggtaatg gtaatgttac gcccctgaac caattcagag attactgtct gaccttgttg
    78241 atcgactgta actttaagcg tttcagtggc tacttcaacc tgaacaccac ctttggtgta
    78301 acccagatcc acaccaccaa atgatacctt gcaggcacca agcttgatgt tttttacatg
    78361 ggtattagac attgatggaa actccttttt ccgttaattt cacgcattca ttgcgctata
    78421 tttaagtata tacttaccta tttatttaat tcaataaata gccagcaaat tcaactggaa
    78481 taccggcttc tattaacgat ccatcatttt tcggataaac gacaggcata gccataggcc
    78541 gtacaagtcg aaaatagacc tctccagatt ccgtttcctc tacgggaaac atatcaatga
    78601 tcttacgcgc tttctcaata gtcgttgtaa ttgacgagcc acgcacaatg attgtgaacg
    78661 actcatgata aaagccctgt aactcatgat cgatactgat accggtattt gggttaataa
    78721 gcaggacacc ggatttgaca taggcaggta agtaatgaca gaaaatgtct gtccctaccg
    78781 tgccaatctt tgccttctgc atcaaatttg caaatgcttc aataaacaca ttaacctctc
    78841 gtaaacccgg cttttttggc agcctcaaga atcgcttcgg agaactgctt ctcgctaatt
    78901 tccaccgctc tttccagaaa gtgtggacca acacgaggtt ttacaccttc aattggtggg
    78961 tttgtcacgt tcttcattcg agaaagataa ccgagacggt atttgcccag ctccatgtac
    79021 ttagcatagt cacctacttc tacgccagga tgcccctgac gtggctttgc tccagacaca
    79081 gaaagctcaa tgcgcagccc tgaataacct tctttaatca ccctagcaaa gatggctgtc
    79141 tccagagatc cggtttccag cggggccatt gcacggctga ggcgctcaac caaacgcgcc
    79201 agcttttcca tatcccgaat aagatatcgc ttaaatgctt tctggctgtt gttgagtcta
    79261 ttccccgcac gtttgaactg atgcgcatcg tatttcagac ccatatattc gcccctactt
    79321 caagatgccc aggtcttcct cgtagccccc agcggcgatg aacactggac acctttaatt
    79381 tttgaccttc aaggatcagt acatcatcaa gttgtacagc cgattccaaa gggacaacta
    79441 acacagcatc aaacagctcc agactcgcct tgccacgact accagagcta tcagcacgaa
    79501 ctgacgattt ctcattactc tgttcgaatt taactacacc gacattcgtc ttcctgacga
    79561 actgcaattg cgcttcaccg tagacgttct ttgtgccaaa gcggtagatc gcaatttctg
    79621 tttgccatga aatattcacg ctctctccct gttgtcgatc tcattgccag ccaaaactat
    79681 tgcttccaaa accaaataat gagaaagaca ttacgaacgt cgaacaatca tacgattgtc
    79741 tatataacgg atcagtaaac gccaggtgct gcgagcaaca tgcacgtttg ctgatttacc
    79801 tgtacgatac atgttggtcg tttcaccgat cgattctgac aaaatgccat cctctcgtgc
    79861 tgcgacgata tcattgccat ttgctatctc acacgcttca ttaacaacgg ctagcattaa
    79921 cgcttctttg aagtagtcag ggaactcttc aaacttctcc tgagtcatct tttcccaatc
    79981 gactaaatca tgccggtacg ctccatctgc tccccacgga atgtcataca cattcagcat
    80041 attttgaggg cgatcgtatc ggtcaaagtc gatacgtaga attttgcgga ttgagaacgg
    80101 taaagtttta atacgtcttg tagcctcaat gagacgcttg cgcattaagc cttcaccatc
    80161 cgacagcaaa gtgtccccat tcagcatatc gatcgcctgc atttgagcat cagcgacagt
    80221 tgcaaacgac tgttctggta tcgacagttc aaaagtattc agcagaacat acatttgacg
    80281 ctcttcatgc gtaaggcccg atgcaacagc cttcacaatg acgtaccgca gatctcgttc
    80341 tttctcagag agctggttat attcagccga cacgacaacc ggaatcgaca tttgaccgct
    80401 ggtaatttct agcggctcgc catcaacgag aatagccccg gtgctgtcct ttactgtgta
    80461 ggtggcagat tcgatatcca gcacgttgaa ggcaaatgaa agagaaacag cttcaccgct
    80521 acgatacgag tcgatctgcg ccattactca ccgccttgtg ctttcatgat gccgtcaatc
    80581 atttcgacaa ttcctttcgc cttaacacca atcttattcc caattaaacg taaaccggca
    80641 ataccctcac tatcagcaat tgactccagt tcttcccgtg tgaaagtttg aattggttta
    80701 gctggttcat ctgccgttcc gcgcttcatt ggaacaatgt caggtgctga tggctcaaca
    80761 atctgatcta cgttcagatc attacgttcg ccatatgcag cagatggaga aacgttttta
    80821 ccctcaacag ttgatgcgcg cattgatgca caaatcctct gctggtcgat aaaaggtaac
    80881 tcggaaacgg ataccccatt ctcaaactta actccacaca acattcccga ataatcagaa
    80941 aattgcggtt ccaataaacg aatttttgct ggtttcatta cgctttctcg ctatacaggc
    81001 atatttaaaa cctacaaaaa gggtgggcgt atagcccacc cttaagcata ggtaattaca
    81061 cacctattat tttagttaaa tttttacatt gttcagtgca gcgatagcct tatcgtgctt
    81121 attcgccaga gagcagtacc atttcacacg agtacgtact gcatctttat tctgtaccgt
    81181 gccaatgttc tccacaacga taccagcatt gtcaccgcca tacagaccgg taagaccatt
    81241 ttcttctgac aaatgcagac aataaatgtt agcagtgcct tcctggcctt ccgttttctg
    81301 ctctgaatct tcagtaactt taaccccatt tttaggaatg aagtcgttaa caataaatgg
    81361 aataccatta tggcacagca taggacgacc gaagttctcc atcatgattt cagacgggcc
    81421 tacgtttact gtacgcagca gcgcacggta agcacgcagg tgctctgaac gcatcatgat
    81481 gcagtcagcg cccaaatctt tcaccgcatc gaccagctcg tcgaacatag agaaagtcat
    81541 agatgcacca gcaatgtcga tcttctgatc tttgtgcatc agacgcggaa taccatcaaa
    81601 cgctttggta ttagtttcag aattacccaa gatcagattt cgacggaatg cacgagccag
    81661 acctttaact ttctggcgaa cctgaacagc caactgattg ttagtatcgg acatagttgt
    81721 tgctaagaat ttatcaacgt caacgtcacc agccagaata cgcagttttg caacatgttc
    81781 gctgaatgtt gctgcgcctt ccgcaatagt atcgtttaca tcaataaagg tcgcttcact
    81841 aagcgttgct tcgcgattat aaagatatgc cttcgagtta atcttcataa aaggcagaat
    81901 cgcaaacagg tcatcgcgat cgatgatagt ttcgatcaca ccctgttcaa gttcattatt
    81961 ggatagcttt tcagcttctt cacgcagtaa tggcatctat caattccctt tgatttagat
    82021 gttacttaag tccaattttc ccaagaccag aggtcaactt gtccatcgtc gacttagact
    82081 tctgttggac taccttatgg gttggtttgc tattagaacc agcaccctgt ttggcttcgc
    82141 tgcgcatgac tgcgtcagcc tctggatctg cccgcacaat gcgttcaatc gcagattcaa
    82201 atgacaacgt gtttccttca ccgtcaacca gaacagctcg atctttgtgg cctgccggtt
    82261 tgtcataacc cactacgcta ccgtcatcac cgacttcaaa atgagaaccg tagatcacgc
    82321 gagctttcgc gggagtcatt agaactttgt cacgaaggaa ggcagaatta ctaaatgacg
    82381 cgccaacggt catctcaact aattgagatt tcagtgatgc gttttcactt tccagtgctg
    82441 cataacgttc gtcacgctgt gccagctctg cctggtgtgc ttcgatcatc tgttttttaa
    82501 cagcatcgaa ctcaccacga cgctccagtt cagcttgctc cgcctcacga cgtgtgtttt
    82561 ctgcggcctg ttcagcctct aaaagctggc gagcacgcgc cgggtcgata tcaccgtact
    82621 gagcaagctg atcggccaat gtgcgctctt tctctttgcg cttcatgttc tccttcagca
    82681 gttcagcacc agctttcttg gttttacgaa gttcggccag taactcttcc tgagtcatgc
    82741 cagcgtattc gtcatcgccc ttcggctgct ctttttgttc accctgtttg tcaggatctt
    82801 gtgtactctg ctcattatca gcagctacac cgccagcgcc tccacgctca tgcgcttcag
    82861 cgacatccat cagaccacga cgggccaaaa gcatttgcca cagattcata aaaattcctt
    82921 ttgttactta tcactcgttc tcttgagtag atgagtcccc attccctcgg ggttgatctt
    82981 gcccgctttc ttgggctgca ccacgatcat aagtaagtac tgacttattt tcaagggtat
    83041 ttagatcatt ttttggagga aaattcaaga gatctttatc aaattccttt ttcatcgcct
    83101 cagtaatgtt cgggaaaatc ttctcaataa gcatttccat ctggtgacga cgtacagagt
    83161 ccggtgcctg aagtaatgac agtttctcgg caacagaaaa ttcatcagta aggccacgaa
    83221 tatcgaaact ttctggatac gcaattaaag agtggtcttc gtccagatct acccccatcc
    83281 atttcgcaac cagtagcatg atttggcgtt cagccctttc cagacgctct gcttttgtga
    83341 caagcaagct atttacacgc tggaagtcat acatttttgc ggcaccagat gaattatcga
    83401 ttccctgtgc gttgtcctgc ttcgttcgct caccagctac accaactgaa tggtagattt
    83461 cgttaatcac cgtcttaatc gtagtgatga tcatctgagc ttgtttcggg tctggtgaca
    83521 gataaaacgg ctggtttcca ccttcagaat cgtaggtgaa gactcgcttt gtgcccattt
    83581 caagcacttt agtgtggttt tcatcaccag gtaaaagcga ctgtaccggt atagccaact
    83641 ggctaaatgt ctgatcctga ataatggcat caaggtttga cagatagttt gcaaccgcac
    83701 gatcaagata agcgatatca tcgatcaacg atgggctgaa atacggtgat tcactttctc
    83761 caatacaatc aacaggaaac acaggaacta cgccgagatt atgctcaccg ctatcctcta
    83821 aaataacttt tgcctgacgg cgacctgctc caccagcgcc cttcttcact tcctcacgga
    83881 acagatacca ctcgttttgt gtccacagac gataacgttg gtattcctga cctgtagaag
    83941 taaaaggatc tgcgtcatca cgagcgactt ccacaattaa cgcccacaac atattcccgt
    84001 cgtcgtccca tgccacatcc agcatttgct gaggtgaaat ccagtaggcg taggcgcgag
    84061 catctttctt tttctcgtca gctactgact caacatcacc actcatcgtg ctatcgacaa
    84121 caacccatat gcgaccgtaa atagacgact gcaaatcaat agcggccata aatgagtcaa
    84181 tagaggcatt ctggcgagtc gcacgtttcc agaaattgcg gatctgctct ggtgcctctt
    84241 cgatatttct atgaatgtct tctttaaaga gatatttgtt gatgaggttt accacctcac
    84301 gagtgtggtt gaagcgataa gcacgttcaa ctcgctcctt aaactcctga tctccctctt
    84361 taaagtaacg aaagatattg tctgtaaacc aaccacgccc gccagcgtaa gtgctggcga
    84421 ggaagtccca atgttctttt ttcttttcgt attccgggtg gcgtcgcgcc accagatcct
    84481 taatttgttt gtcgttcaat tccatttgaa taaccttaga taattactta cctatcgaga
    84541 accaccaaga ataacacgat tttttactgg atacctacga tgtactggat agcccaatgc
    84601 gtctgcacta tgctcaatgc cacctgtctt atccatatct cgtgttccag gcttgtagat
    84661 gactttttcc agtgaatcaa tcaaatgctt gcacttcgga tcgatataca gccggatatc
    84721 tccagatgcg gacatcagca ttcggttaac agcattaaca cgatccgcaa ttggcgggtg
    84781 ctttttcgaa taatcgacac gtaagaatcc cttctctttg aatatgtcta cgtcagactc
    84841 cccacgagca tgttggcgat aagcacctgc cggatctgga aataccgtta cctgtgattt
    84901 ccagcgccag aagcgtctct caagctcatc acaaacttct gccgtgttag aggaaaagag
    84961 caccaattca tcaattgccc atagctcacc atttggctga ggttgcaaaa ttactgaaga
    85021 catcgggtca atgttgaagt cctgccctac ccatataggc aaccgagggt tgaactgaag
    85081 cggttttaca tgcacgttac gatcgaacgg gtagtaaacg cgcccggaca tgttctcaaa
    85141 gctcgccagg tactcctgag cgaacgattt cgggtccata tcgttcttag cagcttcaat
    85201 ttccgccgta ggtacaaacg gagaatcggc agttacaaac tgccagctct tccactgtcc
    85261 ctttctttgc agctctacgt tctggcctat agtccacagt ttgtgaaatt ccgagaagcc
    85321 ttttggcgta ccaatgatca gtgcaccgcc gcgtgtcgat gaaagagtag gtcgtaacac
    85381 cttgtaccag gtgtcagcct tcatatcctg aaattcatca agcactacaa aatgcaacgc
    85441 tacgccgcgc agagtgtcag gcttatcagc acctttgagg gcgatctccg aaccgttctt
    85501 caacacgatt gtcatcgtgg tgtcgttctt tttcctaacc cacttacgag gcagaacttc
    85561 ctgtagatcg tcccacaaaa tctggcgagc catctggtaa gtaggagcga cataccagac
    85621 tctttgcttt ttttcctttg ccgccgcacg aataatagtg gagatcgaca accgggattt
    85681 tccccagcgt cgaccagcac aaacaacttt aaatcgatgc ggagactgga agactttcat
    85741 ctgtccagaa tgcagttgca caagactgag cgaggacggg attgccatta ttcgtcctcc
    85801 ccttcacttc catcatctgt cgcatcaaat tcgcttagag cttcttcttc caacgtctca
    85861 agcaattcgt catcgatgat ttcaggctcg tcgtcttcct gacgtaattt cgccacctgg
    85921 gaaggcgtaa gctcaccaaa taccaggttc ggaatttctt cctcgtcatt ttccgcatga
    85981 tccatgccca atgctttgga cgaaacttca aagcattttg caagagtatt actggctctc
    86041 tgtaagctct tgagagaatc ctcaatcgcc cctaaaggct taccctcacg tttggccgta
    86101 gtgacttcga ccatcaccat ctgccccaac gcatacgccc agccgtcata acgtgtacga
    86161 cgttcttcta tcttttccgc acgggcttta gcgcgaagct ctgcgtcaga tttaagagac
    86221 tcacgaacca tcttcccaac agagtccgcg cctttctcta atcctcgctt tttgaaatgt
    86281 ctggagagtg tttcacgacg aatgccgtac tcttcctcca gctttgagag tgtatattcg
    86341 cctgacgtcc atttggcttc agcttcggcc cactccgctg gtgtcaggcg agtttttacc
    86401 tcgtcttttt cgaccgtcat agatccctct aaaacacaca gagcgcgtcc atgcgctcta
    86461 aaacaacttg tttactgcat atgctaacca acttgttttc tggggtgttt aattaggtct
    86521 gggcatgtct tactaagcct gcttctgtat atatttaata agttacttat tatttatata
    86581 tacagaagca ggtctttaaa taagctccca gaccgattac atcaccagta acttcgcttt
    86641 ggctcgacct aaagtggtta acccaagagt ccggcggtga tagcgattgt cactgcgttg
    86701 gcgcgtatgt cctttctcca ccagcccctt ttttatcaga gcgcgaattg agaactggat
    86761 gctttgcttg gttgtcttgt acggcaaaac ttcaagcaat tcgtccagat caagtagatg
    86821 acctcgctca taaccgagat tgagcgtttt gatgatgtcc ttttgtttgt cggttaacgt
    86881 catggcaaat ccttatgccg gtaaagcaat ttctaacggt ttatccaaag gttgtttgtc
    86941 gaatgccagc agtggcagcg tgtcaggcag tcgacgacca aagtcagggt ttcggtacac
    87001 accatacaac ggagacgtaa agctcaggtt gtgaatgtcc ttgagcagct tcacaatgct
    87061 ggcctcgtcc accagactgt cggcaatgtc ctgaatcgtc gtgccacgat tccgcccagc
    87121 ttttgccagg gaactgttct tgtggtagtc cgccaccaga tcacgcagtg cacggcgacg
    87181 acgagactcg ctcattgcga acaactcttt gacgatcgcc tcgttatcac cagggtcgga
    87241 acggaaatgg cgctggaata cacgcagtgc actttcatag ctcttcggtc gctcagggcg
    87301 gatgaactta aaccctgctt tcatggcgaa gggattgtat ttgctcatcg aggactggat
    87361 ctcaatgatt ggccggtcat gcatcctgct aaccaggtta atcattcgat aagagacgcc
    87421 tacgccacga tactgagtat ccacaactga gcgactgatc accgcaaagt tgttattcac
    87481 gtaccgaccc cagtactggt tagctacggt ggtattagtg gttggtttca acttaggaaa
    87541 catgcggtgg cgaggtgcca acagtagttt cgggtaagcc ataaccacga cgcccaccag
    87601 acggtcatca agttcgcagc gatagtaagt tggcgcgaaa ggtttgccgt ctgttttgta
    87661 gtgaagcgac ttaagagcgt gccagtcctc tacagttccc tttgtgacag tcattcgctc
    87721 cagaaagtcc agatgacgcg gaaactcttc agggcggtag cgtttgatga tgatatctgc
    87781 catgtcgatc acctgcgctc gatattggca ttgatgaaat ccaggcgaag agactccatc
    87841 gcccctacca tgacatacgg gcgcccgccg ttatgccagc aatcaagaac actgccgtcg
    87901 ttgttgatca tcaacagcgc caggctctgg ctttttcctt ctctggcgtg ctgaagtgct
    87961 tcttccagca ggcggatgac ttccacgttg ttgttatcag tctctttcga tggcttcagc
    88021 tctacgatct tcaaatcagg catattccac cttcacgcgt tctttgtagt gcttggtgat
    88081 ctgcatatcc gggcgcagcg cgttcttcag gtcttcgtga gtcgtcgcca ccattaccgt
    88141 cgcaccaacc tttcgagcgg cacgctggag gttagacgcc acaacctgag cggttacacg
    88201 gtctagaaca gcgccgaact cgtcagcagc ccatacttta gcgcctgact caatcagttt
    88261 ggcaatcttg agacgatatt tctggccgtc tgacatttca gaaggcttgc gaacaaacag
    88321 atatgcatcg ttcagaccag ccatagataa caacccaagc gcatcactgg tcgttttgcc
    88381 caactgatcg atgacgttaa cctcattatc gaaggtaaaa tcatcgatgg aggctacaga
    88441 cagcccttca tccttcatct gtcgttgcaa ctcgcgcagc acaacggatt tgccggaacc
    88501 ggattggccg gtgatgtaca ccacatcgcc ctgcttcact tccagctcca gattgtcgta
    88561 aagcgtccac tctttttcgt ccaggccaag cccgaacgac tcagcgattt ccaacgtgcg
    88621 cgtggtttta tttacgcgtg tctgaaacga tacgttgatg atgtatttgc tcatgcagcc
    88681 atctccccgg aagagatttt ctccgcatat gccacaaatg cgtctacccc gctttctccc
    88741 gtcatttctt ccatgtgggc aagcaaatca ccaacaacaa tggcagagcc agcaggaagc
    88801 gttttaaagc ccaatacgtc gacaacacgt acttcttccg ctgcaacttc acgactgatc
    88861 tcggtgtgtt catccttctg tcgtttagtt tcttcgccaa gatcgataac tagcgagtcg
    88921 gtgtccattt cttctgtcat actgccaacg agaacattca actcacgctc ttcgaagccg
    88981 aaaacctcga tatcgtccag aacaagcgac tcaagctctt tctgtagctt aattgcatcg
    89041 taatcaatgc tggcaagtcg gttatcttca aggcgcttcg cacgaacctc gtcatcactg
    89101 agatcatcgc gaacaataac cggtacacgc tcaagtccag caaaaattgc agcctcacga
    89161 cggccgtggc cagtaataat tacgtcgttc ttatcgaccg taattggctg gtcaaatccg
    89221 cgctttttaa tggctgcggc cagatctctg atctgctgtt catcatgctt tttggcattc
    89281 atctcatagg gaataagatc tgccgggtcg cgatatacga tttcaaactt tttggtcatt
    89341 acatacgctc cttgtagtag tcgaccagcc acaccagagc ctcaccagcg ttctccattt
    89401 cattaccggt gttaatagcc tgctctttga tgatgttttt tatggtttct gcaacacgat
    89461 ctgacgcatc gaaagttact ttgaagcgca tggtctgatg ttccgccccc acacgttcgg
    89521 ttttctctcg tttgtcggta tcgacaggct catcactacc acgagacaac gcctccagtg
    89581 cttcaaggtc gattgccgcc tcttttgcta aaaccatcga gatttcgtcg tcatacgggg
    89641 cgatttcaga cagttgatag tcaagttctg actgaatttc ttcaatgaag cgttgcaatg
    89701 cgatttggtc gtcttcaccg tatcgctcgt tgtccaccag tgacatctgt ttagctacga
    89761 catcgctaat tttgcccaca gaaagcactg gaaccgttga aattccttgc tcaatagcgg
    89821 cacgccagcg atgttcgccg ccgaggattt caaaaaatcc atcttcaagt tcacgagcca
    89881 aaattggctt aaaaaagccc aatttttcga tagaaccttt cagtttttcg aaattctgcg
    89941 caccaaccga attggtgttc caggtattcg ggcgaaggtt ggcaacatca acctgcaaaa
    90001 tcgtgatttt tacgtccatt ttattgctac aatccactaa gtaatcactt acttattata
    90061 atagccaaat aacatacaaa aggcactaag gaaagaggtt tatgactgtt cggattgtat
    90121 ctaacgcagt aaatgcgctt atttctggcg cagatgacaa ggtaaagcaa ctggtgcagc
    90181 aaatgttgag ctacgaagtc gaggctggcg actggaaggg cacaagcaca atgttcaact
    90241 ggagcaaaaa ctcgttccct gctggctttg ccaagcccgt ggcggctaat ttgaacaagg
    90301 cgggcatcaa atgtgttcat atccgcaaag acaaagcccc ggcgcttggt aaaccaaatc
    90361 cggcggttaa cccattccca tacaatcctg attatgcgta tcaggatcag actgtggaaa
    90421 cactggttcg agagggaatg atgattgcgc agatcgctac tggtggcggg aaatctaacg
    90481 ttgcctgcaa agcagctgca cgtatcggtc gaatgacatt atttttaaca acccgctctg
    90541 ttctgatgtt tcaaatggcc gaaaacttcc agagatccat cgactaccgc gccaaaaatg
    90601 gcgaaccgtg gttaaaagac caaaaggttg gagtcattgg ctcgggtgag ttccaggtat
    90661 cacgacatat caacgtcgct acagttcaaa ctcttgcaag tttcctcgaa gaaccaccac
    90721 gcgatgcaac accagataag aaaagctacc acctcaaacg tcgggagttg gtgaaacgct
    90781 tcctttcaag tgtctctctt cttattctgg aagaggcgca tgagtcttca ggctcaaatt
    90841 tctatgacat cgccagatta tgtgtgaacg cagactatcg tctggcgctt acagccacgc
    90901 cgttcatgaa ggattcgacg gaagccaaca tgcgcctgat ggcggtggcc gggcgaattg
    90961 aaattaaagt cacagaaaag tatctgattg atcgaggcat tctggcaaag ccgtacttcc
    91021 tttatcataa agttgcctac aagccagacg aggccagaat caaggccgaa cttgccaaca
    91081 aacacctcaa ttttagagtt ggtatgagca ccgcctacca aaaggcttat cagttgggga
    91141 tcgtgtataa tttgggacgt aacgaggcca ttgtgcgcga agcattgctc tataagcaac
    91201 attctctcaa ttgtatgact ctggttcgcc ttaaacgcca cgggcaaatc ctgatggaaa
    91261 tgatgaaaga gtccggcctt agagttgact tcatctatgg ggaatctaac caggcgacaa
    91321 ggcaagcaaa gctgaacagt ttagcgtctg gagaaataga tgttttaata ggctcgacta
    91381 ttctggatgt cggtgttgat gtgccaagcg ttggtgcggt cattcttgct ggtggtggga
    91441 aagcagaagt tgaaatgagg cagcgtgtcg gtcgtggctt acgagccaag aagaatcagg
    91501 caaacgtgtg ttttatcact gatttcattg acattagcaa caaatacctg ttgtctcact
    91561 cttatgagcg aaaacacatc atcgacacca cacctggctt tgcagaaggt gtattgccta
    91621 ttgatggcgc attcgatttt ggagttctga aacgagatta gttatgagcg aaaagaaaac
    91681 aacttattgt caggtggcat tgtccgataa ggccaatgac aaacttggaa agtttcaaat
    91741 gaaactaaaa gaaaaaaaca tcaaaatgtc taaggctgaa gttatcaata ccattctgga
    91801 gacgatgacg atggctgaat ttgacaaagt ttcatctgcc gttggagtct ctgctaaggc
    91861 tcgtgagaaa atcatgcgca tctatgagaa ctctaatatg acaaaggaag atttagagca
    91921 aattctaagc aaactgccgt aatattggat tcttagtcaa tttcagaatt gccagacttg
    91981 aagagctaat agacagagtg cggcaacaac gcctttgccg cacagctttt agagaaaaca
    92041 cgtctgctat ttacagttga aaacttatcg tttgagacta ttggatattt ataattctac
    92101 tttgcaataa tcgctgtata aaaatgaaac gttcgttact tcttgcttgt atatacctct
    92161 cgtcattttc cgccaatgcg ttgtcagata tcgtctccga catgcaaaaa acgtacagca
    92221 tgtatacttc tgaagacgta ctaaaatacg aatcacgagg tctgattcaa ccagtctggt
    92281 tcagattctc agataacgca gaagaaacaa tacttaatgc aggaaaagaa agattacggt
    92341 tgcaaattaa cggaaccgct gtaccagaaa acgttaatgt aagctctgac gaagccatga
    92401 agccatctgg aattgttttg gaagttcaag acggcacact ttactgtgat gaaatggcta
    92461 caacaggttg ccaagtaaac attagggttg gcaatgaaga gcctatcgtt acttacgcca
    92521 aggctgaagg gaatacaagt ggaaaggtat tgtttatcga tgaaaatacc tcaaaacgta
    92581 tcgttcgtga catacagaaa acagcgaact ctaaatccgt cgataaaaaa atctatgttg
    92641 aggttccctt ctttgtcgtt ggtaacaaag aatttaaatt tgggttgctg aaaacacctc
    92701 taagagtatc agaatgagaa cattggacat cacctacggc ataccggctg aagtatggcc
    92761 gcgagactac gaacatgctg aacgtacctt gctcttctgg cggagtgcaa aatttcctgt
    92821 aagagtcact ctcgaagacg gccaagtgtt ttgcatgtat gtctacgggc tattgagcgc
    92881 aagaaataag ctgaacctca caccagatcc gcttagcaac acaaatcgta taaggctccc
    92941 tcttgagcgc attagcacaa ttgagtcggg catagaagag gttgacactt cattcacagg
    93001 tcgactaacg ctaaacaatg aacagatggt taatcagcca tcacgtcgtg acttttttaa
    93061 gatatgtcgt caggcttacc aaaacggcaa atctataagg gtctacatgg cagacggtcg
    93121 cgaaattgag ggaacgtcag atggcgttga cgcctgtcta gtcacactta agttggaaga
    93181 cggtagaaaa atacttattt tcttcgattg ggttgaacga atccttccgt tttaatttct
    93241 ataagggtaa taaccggcta agtccggtta ttattttaca tgccattcat tttatactct
    93301 ttcaattcta ccccgtataa atatataagg tctaaagccg gaatcgatta tttatttagg
    93361 gaacaccctc gacgatctaa catttatttc taggactttc atccctgcaa aaaaacttaa
    93421 aaaaacactt gcaatctttt ttcgcgtatc gataattgaa cgcatcagga acgagtgcaa
    93481 aacactttct ttcttgagtt aaattttaat atttatacta aggaataaaa tcatggctaa
    93541 cattatcatt tctaaaaagt ccatcattga agctgcatct attgtatccg acgaactgcg
    93601 cgaaaaagca gatctggcaa ctcaaacata taacgaacat tataaaaatg gtacgcacac
    93661 taaagcagac aaagcaaata tgcaagctgc gaccactaaa cttgcttact tcatcaacaa
    93721 cgtcgtaaac gctgtagaag acgaaaaatt atgctctgtt ttctactatg cgattaaagc
    93781 aagcaaacaa gcgccagaag tattttttcg tgacgctatg actaatagtt attcactgga
    93841 aaaactggtt tatctggtta aatcaattaa agctggaaaa tgtgtttatt ccgtcgctga
    93901 tatgtctgga tctcgtgtat tcgctttaat cgatatgatt aacgacgaga tcgacacgtt
    93961 cacgaatggc gctgttttcg atttaatgaa tgaagccaaa aaagcgtgtg aaattaaatt
    94021 ggacgctggc tatactcaag ctaaccagct aatcaatctt tgcgaacgtc tcggacttgt
    94081 tgagaaagtc aaaggaatgg gaagcgccaa agctggtaca cagcaatatc gctttatcaa
    94141 gaatgatttc tacaattatc tagctgacgc tttcaaagcg taattaatgg aatcaagcgc
    94201 ccactatggg cgctttaaag gagctttaaa aaatggtcag ctatgacaag atccgcgcgg
    94261 aatatcgcgc aaagtatcgc gcttataaac tagaattaat caatgattta atcgcgcaac
    94321 gcgaccaatt aaattttacg ttttctgatt tgcttaacag caaacgagat tgcaagcgta
    94381 aacgtgaata cttacgttta agcgcattaa tcggaaaact tcataattct atttaatttt
    94441 tttaaggagc ttaatcatgg ttattttcat ctctggcgta aatatcaata atcatactct
    94501 tgtttatgat attgctggtt tagctggata tgctctaagt tctgaagtgg ttgatgaaac
    94561 aacgtttaaa ataaacatca atgacgtaga acatagagcg cgcgcaggaa tcaatgaagc
    94621 agatgttact ttaatgcttc aagaattttt aaacgctggt tttaatattc gcttagaaaa
    94681 ataaaaacaa tagcgcccat agtgggcgct tttttcgttt ccaatactcc caccataacg
    94741 cgccaatgat ggcgcgtttt ttattgtctt tgactcactc caacaacata aaaataagcg
    94801 ccaaaataac gccatagacg cgcttttata tccttgccag tacataccca ttacttaaca
    94861 cattaacgcg cttaaaacgc gttatattgc gctatagcgc atagttaatc attggctttt
    94921 agtcttgcta tgtgatccgc gtttatttgt cggcgcggat cggcattttg ttttgttccg
    94981 tatccgctcg cgtattatgt gcgcgtgatt tttcacataa tcacaccact taatcacgta
    95041 tgcgattacg ctacgaaaga aatctggatg tctcaggcga cgaaagtcat cataattttc
    95101 cccgctcacc tgtccgacaa ccgctggttg gattccacca gcttcccgat gttttttcta
    95161 cataaaggcg aatgcagccg tttcccgaaa aaatcctggc cgttccccgt cggttcatga
    95221 atacgttcct cgccgtttct gaaaatttcc ctgcggcatc tggtggctat agagaaaggg
    95281 ccgttactgg ccctcttctc agttacacgc catcaaggat gtggatgcgg ttgcttgcgt
    95341 atacattcag cataaagtta gcgcaaaaca gtttccatgt atcaacgcca gcggtatacg
    95401 taatgttttt gcatttaatt gcattgttgg cgatccgcat cccctgcgat actgcttcat
    95461 cgtcggaaaa tttgaacgac gattgagttt taatccagac agaaatctgc gtagcgaact
    95521 caatcagctt ggactggcag aatcgcccgg agcgcaccgg aaagacgaac gttccgaatc
    95581 cagaatttac cacatacgct ttctcaaata cccgcgagta acgacgattg ccaatgatgt
    95641 cgcgtgcaat aatgcatttt tcttgtgctg acagttctac cgtctcattg tcgcgccatg
    95701 caccaagtac tcgtttttca atgtcagaga acgttacagc gatattgcca tgtgcgggtg
    95761 cgtttacagt agcgataaaa ttcatgatta attccttatc gtaaataaca aattgttttc
    95821 ttgttggcgt taattatcgt tgtacgaata aggcgtcaaa gtggaaagtt gcggtagccg
    95881 gacgggaaca ggtgggtttg tcggttgcct ggaggtaaga ggttggtgtt tttagcctgc
    95941 gggaaacagg atggtcattt aaggccacca gcagtggtgg ccttaataat tatcgaccga
    96001 ttacgctaag gatcttttcc tcaactgatt tgttgttgcg actaaactgc cttgcgtatc
    96061 tgatgacaga agaggcgttt tgctttctga ctatctcggc tctttccttc aggcgtttct
    96121 tgaaatcgcc catatttacc accaggcgca gaaggtcagt ctgttattgt gaaagtcgtg
    96181 gttcttaatc agctcttcca caagtttttt caactccccc acgtcatgcc agtacccttc
    96241 atcgtactcc tgactgccga agaaaaaacc ttcacaggta ggtaagtact cttcgcagtt
    96301 actttcgttc atgtgcatca aatgagcttt cagagcacaa atgtcattca tcgttaattc
    96361 taaaagctca caatttttga cttcacctac gttgcgttcc atccacccaa agagagcatt
    96421 gaacttacgg aagtaagcaa ccttctttct ggatgcatcg ttattcagat cgttttttgg
    96481 ttgcgtctca atgtagatat caagtcccat gatggtttcc ttatcaagtt gctttcagtg
    96541 aaagcatttt cacaaatcag ataaggcaga aaacaaattg ttatcgggca taagaaaatg
    96601 gcgcggttta cgcgccattc aaagaggatt aagcgaatac gctttcaggg attaaggtgt
    96661 cagcaggaat gccggaggta atgcgcagac catactgacc aatccaggta gtgttagtgt
    96721 tcagactgga agcgaatacg gtgttaacca tattcatcat ggtttcaaac acctctttat
    96781 tcaccttacg gaagtaactt tcaattttca acaacaacgg gtccgttgca tcaaaaatgg
    96841 actgatattg cgcctcatat tccccgcctg atgtatctcc tttacgcaca atagtacttg
    96901 cctgaacttc agttccagtt tcggtgttgt acgtaacgac ggtgatttta gctacattct
    96961 taccttcggc gctttctgaa gcatagtaga catcaactgt caggttttct cgtttaacgc
    97021 tcatatcact ctccatagtg tcttggttat ttttctattt tatagacata ggtatgtact
    97081 tacaatacaa aaaaaccccg taaggatggc ggggctgtcg tactgtcgac taattaccac
    97141 ttgcacatta tcaggctatt gccatcactg atgctctctg aatctcttgc tgggccacgc
    97201 ggttaacttc catcaaagcc agttcgagct gatgttccgg ccacattctt tgaaatgcca
    97261 tccagccttt tccacgctgg cgacgaacat tcattacata acgagtaaag gtgtttttat
    97321 cgaaagacac cgctgtttca cggaacagac gaatagaggt tccattggca acgatgtcca
    97381 gcaaagttaa ttgagcaacc agtgcaggct tctcttttct cttctggtct ttgtccagac
    97441 caagaaccaa tgattttttc atcacaactc cgtaaacaac ttgttttctc aatggcgtaa
    97501 ataataccag taagaaaacg gctacaaagc atattgatca ggcgcatgag cgatatgcag
    97561 gttcttccct gcggctacag atgaagttaa gccagcaatg ctggctttgg ttgggcttac
    97621 gcgaatatcg caaggaagtc ctgaacgtcc caatgctgga cgaggtgaag atagacctta
    97681 gaaatcacgt cattttcgct atcgcatgtg aagtagtact tctggctgcg gttgtatgtg
    97741 tccacacaaa attcgctcat atccataaag tcagcatctt cacccatgtc gcaaatcatc
    97801 aaagcaggtg cgtcatcacg agtcatcagc tccagcaccc atcttgaatt aatcagagtg
    97861 gaagaccaac cttccagctc ataaaactgg tcaaactctt tttgagtcaa tttcttaatt
    97921 tggttgatgt cgatatttgc aaacataaag caatccttaa acatgttgtt ttctgaatta
    97981 cgtaaacaat accagtaaga aaacggccac aaagtatatc gttacggtgc tgcgctgccc
    98041 cgaggttaat agcgacaaaa gccaccgtgc tggtggcctc actcttaatc ttggtcgtcc
    98101 gggaactcgc tgtgtaccgc gcccaccagc tcccgctttt catcttcact cagcaggtgc
    98161 cagatatcct tccctttggg cgattcccct tctgccggga cgaacgacca caacttgcgg
    98221 tacaactccg gccccactgc atccagacac tcagccaggg aatccacgcc ccacacctca
    98281 accgtcacag gcatcttaat catctcttca tcctcacttc gtttctataa cgtctaaata
    98341 tgctgtttac ttgttggtgt tattatcgca ataagataaa ggcgaaaaag gattttatgt
    98401 cgggaaagcc aggctgggca gtgaatatcc gggaacgaca cgagagccag tatttacgat
    98461 attctgctct acgcgcgccg cggggctggt gggtagaata atcctctccc cgaaaaccta
    98521 ctgaaatcga ttgtgacccg ctggctgtct ggcacaatta cgctgtatct ggcattcaga
    98581 gggaatccag cggtaagcaa gtcagcctgc acgtgagcgc ctctcttctt cccgcacatt
    98641 acaggtgggt agctgcaact ctctctctct cgctataccg gtacgccgcc gttccagaac
    98701 cggcatcgag ccttttcccg aaatccacac aggcaactcg accgtttccc tgaaaatccc
    98761 cagccgttcc cccgaaggca acccagccgt ttctgtagct ggatgcaacc ttttccctgt
    98821 atggaccgaa ggctgaccgt agggggtgtt acgggggata aaatcaggag ttgaataaat
    98881 acgaaaaagg aggcgacccc cctccttccc tctccactta tacatacctg gcttcttcgc
    98941 tatacatgtt gtgttttatc tgtctcaacc tctctatagg cgttaatgct cactccttcc
    99001 agaatgttct atacggcgac tatttctgtt ggtgaattgg gtgtgttgtt ttctctgtcg
    99061 gtgtatgtgg tttcgttagt tttgcggtgg ttcttctatg ggggtgttct cgtcgttttg
    99121 gtgattgttc ttctccgtgt atggagtaat ggcaggtggg cgattgcttt cgttattgtg
    99181 tttgagttgt ttgtctggag tgtgtgaagg cttttgtagt tattcgtgta ttgggtaatg
    99241 gcgtttcccg tgattgttat gtttcgtgtc ttttgggatt gagagaaacc ttgcggttac
    99301 aggcttcgtt agggtttcag cggttcaggt gtttcttcgc tgtttgggta atggcgtgtc
    99361 gtgggtatcg cgcgtatgaa tgttttcgga ttgcctgtga aagttaggtt ggttgtccgg
    99421 tagcctgggg agaagaggtg ggtcttttcg gtagcctgcc ataaagaggt tggttgtttt
    99481 gggtaatggc atatgtgtta tttggttgtt ttgttatgcc tgaaaataag ttgtttatat
    99541 ggttgtgtaa cgcaacggga gcgattttga gcgtgtgttt ttgttggtag tgtgttgagt
    99601 cgtctgtggt gaaatttgga cgctgtggcg ctatctgctg cgtagaatta cgggtataag
    99661 aagtgatgtg tgataacgtc aattttttag accaaatcgg gagaaagcgt tgatgtttcg
    99721 ttgatctgtt atttagttgt tttcttattg gtataaacaa ccataaccta ttgatattta
    99781 atgatgtgat tgctggtatg ggagggtagt agtggcgatc aacgggttct tatagaaaag
    99841 aatcaaattg tgaccgccat tatcaacggt ttgattagat gagatggtct aattcatcat
    99901 ctatcatggc tttgacgctg gagtcgttca ctccatattt atttatgagc tgtttcagct
    99961 ctgtcgaaaa ctgaaggtag atatcgtgag cgtttttcac tgtaggttca acgccactca
   100021 tttcaagcgc ctttagtaga tatgagtctt ctttaagcat aaattctccg ttatctagta
   100081 tgaagcgatt gccggattac cattgccagc actcaagccc ttcaacgacc ggtgcgccac
   100141 aatcaaaatg tacagagtta aagcctacat caagtacttt ctgaatgttc gcaatcgcac
   100201 cctcggatat accatatcga cgcaactcat ctttccagtc atcgtcctgt aacccggcgt
   100261 ggacaatgca accgtactca atttgtgaaa cccaatccag ctcattgctt gcgatcatga
   100321 atcggcgagc atcttcctct gatgtatgag atgaactgat caatgccacc tggtaggctt
   100381 gggtaatgtt caacatttca actactccaa aacaagttgt tttcttatgt ggcttattat
   100441 ctcaatcaac aaaaggtaga aaacaaattg ttttagggct tgtgtaaatc aggagggatt
   100501 gtgtctatat gaaaatgccc ggcgtgttgc cgggcattgc gaagagaggt tgtttaatta
   100561 cttaacctgg tggccaggtt tagcaccagc atccgggctt aacaggaaga tatctttccc
   100621 gccaggaccg gcagccatca ccatgccttc agagataccg aagcgcattt tacgtggtgc
   100681 caggttagcc accataatgg tgtgacgacc aatcagtgcc tgtggatctg gataagcgga
   100741 acggatgcct gagaagacat tacgcttttc accaccgaga tccagcgtca ggcgaagcag
   100801 tttgtcagaa ccttcgacaa actccgcgtt ttcaatcagc gccacacgca ggtcaacttt
   100861 ggcgaagtcg tcaaaggtga tggtttcctg aatcggttcg tcagccagtg ggccagttac
   100921 cggtgcagct gtggctttca cttcttcttt ggatgcttcc accagtgctt ccacctgttt
   100981 catatcgata cggttataca gtgccttgaa cggattcact ttatggccca gaagcggttg
   101041 ctggatacca tcccaggtca gttctgtatt caggaatgct tctgcacgtt cagtcagttt
   101101 cggcagtacc ggtttcaggt acgtcatcag cacgcggaac aggttaatgc ccatagagca
   101161 aatcgcctgc agatctgcat cgcgaccttc ctgcttcgcc accacccacg gagcctgttc
   101221 atcgacatag cgattagcca ggtcagccaa tgccatgatt tcacggatag ctttaccaaa
   101281 ttcgcggctt tcccatgctt cgccaatcac ttcagcggca tcagtaaagg ttttgtacag
   101341 ttgcgggtca gccagttcgc ttgtcagcac gccgtcaaaa cgcttgttga taaagcctgc
   101401 attacgggag gccaggttaa ccactttgtt cacgatatcg gcattcacgc gctgaacgaa
   101461 atcttccagg ttgagatcga tatcatcaat gcgcgaagag agtttcgcag tgtagtagta
   101521 acgcaggctg tcagcgtcaa aatgattcag ccaggtgctg gctttaataa aggtgccgcg
   101581 agacttggac atcttcgcgc cgttcaccgt cacatagccg tgaacaaaca ggttggtcgg
   101641 cttgcggaag ttgctgcctt ccagcatggc aggccagaac agactgtgga agtaaacgat
   101701 atctttgccg atgaagtggt acagctcggc ggtggagtct ttcttccagt attcgtcgaa
   101761 gcttacgctg tcgccgcgct tgtcgcacag attcttgaaa gaacccatgt agccaatcgg
   101821 cgcgtccagc cagacgtaga aatatttgcc cggcgcgttc ggaatttcaa aaccgaagta
   101881 aggggcgtcg cgggagatat cccactgttg caggccagat tcaaaccact cctgcatttt
   101941 atttgccacc tgctcctgca acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa
   102001 agagggcaga tcaaagaaga agtgttcaga atcacgcatt accggcgtag cgccagaaac
   102061 caccgatttc ggctcgatca gttcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt
   102121 atcgccgtat tgatccgggg atttacattt cgggcaggtg ccttttacaa aacggtccgg
   102181 caggaacatg catttttccg gatcgtacag ctgtgagatg gtgcggtttt taataaaacc
   102241 gttttctttc aggcgagagt agataagctc ggacaactga cggttctctt cgctgtgcgt
   102301 cgagtgatag ttgtcatagc tgatgttaaa gcctgcgaaa tcagtctgat gttcctgact
   102361 catttcgcca atcatctgtt ccggtgtgat accaagctgc tgtgctttca gcatgattgg
   102421 cgtaccgtgg gcatcgtcgg cacagatgaa attaacctcg tggccgcgca ttcgctggta
   102481 acgaacccag acatcagcct ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt
   102541 agcgtacggc agcgcgcacg tcaccagaat tttcttcgcg acttgggtca tagtaggcat
   102601 tacttctttg tagtgaaaag gggcttgata gtaacaaaat ggccttatgt ctgccatgtg
   102661 ataacagcat ttctcataaa tgattaaatg tcgtagctgg agtacactac aaggcgacaa
   102721 ttgcgcaaat taaaataaag gagacgggat gagcgagtcc aaatcgccgg atgccctgag
   102781 agcaatggta gccggtacgc tggctaattt tcagcaccca accctgaaac acaacttgac
   102841 tacgcttaaa gcgttacacc atgttgcgtt gatggatgac acactgcatg tcgaactaat
   102901 catgcctttc gtgtggaata agccttttga agacctgaaa gagcaatgta gtggtgatct
   102961 gctccgtatc actggcgcaa aggctattga ctggaagctg tcgtacaaca ttgccacgct
   103021 taagcgcgtc aaaaaccaac caggcattaa tggcgttaag aacattatcg ccgtcagctc
   103081 aggcaagggc ggcgtgggta aatcctccac agcagtaaac ttggcactgg ctttggctgc
   103141 tgaaggtgcg aaagttggta ttttggatgc cgatatctat ggtccatcaa ttccaaccat
   103201 gctgggcgcg gaaaaccaac gtccaacctc accggacggt actcacatgg cacctatcat
   103261 gtctcatggc ctggcaacca actctattgg ttatctggtc accgatgaca atgcaatggt
   103321 gtggcgtgga ccgatggcga gcaaggcgct gatgcagatg ttgcaggaaa cactgtggcc
   103381 tgatctggac tatctcgttc tcgatatgcc accgggcact ggtgatattc aattgacgct
   103441 ggcgcagaac attcctgtaa ctggtgcggt tgtggtaact acgccgcaag acatcgcgct
   103501 gatcgatgcg aagaaaggca ttgtgatgtt cgaaaaagtc gaagtgccgg tactgggtat
   103561 cgttgaaaac atgagtgtgc atatttgcag taactgcggt catcacgagc caatttttgg
   103621 caccggtgga gcacagaaac tggccgagaa atacaacacg cagttgttag gccaaatgcc
   103681 acttcatatc tctcttcgtg aagatctgga taacggaaca ccaaccgtta ttagtcgtcc
   103741 agatagcgaa tttacggcta tttatcgtga cctcgcagat cgcgttgctg cccaaatgta
   103801 ttggcaaggt gaagtaatac ctggcgaaat cgcattccgc gcagtgtgat aaaaaggcgg
   103861 tgcattgcac cgcctaatca gttctcctac atcacaggac aatcatcaaa ctcgccactt
   103921 cttgcgtcgt tgataatgtg agtgatcaca ccaaacacgg cattgctgcc cgtgcatcca
   103981 tcgtcatcct ttggcaatgc ttccttctta ccggttctta aatcctccag atgctgacgt
   104041 ggagacttcc ggtatctttt tacgcgatat tccccatcca gagcgcatac aagcagagaa
   104101 ccatcaaccg gggtaagtga ggaatcaacc accagcaatg caccctgtaa tattccctca
   104161 cgatgatgac tatcagctgc tctcatgaag taggtcgctg aaggatgtct aattatctgc
   104221 tgatcaagag aaatacggct ctcagcataa tccgccgcag gagaagggaa gcccatagcg
   104281 ttttgccctc aatactgttc atttacacag tatacatgaa agaagctcac tcagtgcaac
   104341 tgatcggtac atagcaccag acaaaggaac gttattttct cgaacaagaa aaggtagaaa
   104401 acaaattgtt taaggccaca actagtggcc ttaatggaga agggatgctt attggctatg
   104461 gctaaaaatc aaatgagaca cttcatttgg ttgtagctcg tcaagatccg gggccacaaa
   104521 accttttccg aaagacttca caagtgattc accagccgca tcattgtcac ctattacgcg
   104581 aaagtcatac ggcagcattg ccagttgacg acgtaatcct gctgagaaag tcgaacctaa
   104641 cagcgcccac gaatttccgc ccgcgttgtg cactgctgca cttttaaaaa tcgactcagt
   104701 tatccatatc ggtccatctt ttaaaggaag ataggttccc catagtagct gtctgccgtg
   104761 cgctcgtgtg aaataccggg ccatcttcgg attgctatgc tgcttaggag cgcctggtct
   104821 gtatgtctga tacccggcca acatcccatc aaacctgtat agagggaatg taacaactcc
   104881 ttcgctttca tcgatccact gtctatatgg atgcacatca tagcggtagt tacgagttag
   104941 caaatgagct tccagctttc caaatttagt cataacttcg catccctcat aacacttccc
   105001 atttatcacc atatttctcc atagctctta gccaataaac atggccacta ggcatagtga
   105061 tgtgaggctc gccaaactct tctccatgta catcaaagat gtccccaaca gaaaattggg
   105121 ggatttctgt tccgttttca cctatatgtg taattcgtat acgtttcggt tgggattgtc
   105181 cacctttgag cgagttgttc actgctctgg caattttctc ccgcaattgc tgcgttccgt
   105241 gatacgtaac cgcaatatcc cgcaactcat tcactagttc tcgaatttgg tgatctttaa
   105301 gctcattatt tgggtgattg gtaacaactc tggttaattc cccattgtct tttgctctta
   105361 cgtatgcagc tcttgtgcat tcgccccatg tattaccgcc tacattaact tcgtagatta
   105421 ggctggactt atctgtcaat tcggaacaaa tacgggccgt ggtctggcaa ctagagcatt
   105481 cgcagttatc gcggaaaccg tggtctactg gcttcggttt aacatcatca actttttcta
   105541 ccctattgga atttaaatac ttcgaagcct tgctatgcca atcgctggtt ttaggatctt
   105601 gtccatccat caaacgccag gcttcagcta ttaactcata actagtgact gtagcttcct
   105661 gcgcatcacc aataagtcgt gtaatctctc tttcaagccg accgcctaac tccgttttag
   105721 tgcaatgttc tgaccaatcg ccagtctcaa gaagcgcgag aatgcttaaa atatcgtcca
   105781 atagaacggt agtattatcg acttccttct gaaactcacc ggctactgat ttatgcatca
   105841 ttcgtcatcc tcatccgagt catcctcatc acaggatgag agtagtgggt tagtcattcg
   105901 ccctacttga gtggcgtagc aacgccgaca gagattgcgc agcacactgt atatttcgaa
   105961 catctcggtt cgttcatcgc caacatcaag ctcagatgct atagcgtggc attcagtcgc
   106021 gagagccgat attttctgaa ataactctgc tttattcact cttcgactcc tgcggaggtt
   106081 ctggtagcgg catccagtga gttgcttgct caataccatt acccggctta atcgttacat
   106141 ctccgcgccg aaaggtgatt ccggtatagc gtgcggagca tattagcggt tcaaccagag
   106201 agctatcgaa attcaccgaa ataagcacgt tctggccctt ttcaggcatt cgctcactac
   106261 agcttatcca accatccgga gttaccggat ggggtccagc gaactcggga atgtcaggac
   106321 cttttctgat agctttagcc agatccagcg ggtcatcgta aagccagtcg ccagtttgtg
   106381 ggtgatttgc ttctgcaagc tgcgcagccc attcaagacc atctttttga ccttggagat
   106441 aatcaagcgg caactcttca tgattacttg caggttctgc actatcagct tcgcgccgct
   106501 tctgtagctc tgctgccatc gctctcacga cttcaactgg tgcccttgca gcaaactcta
   106561 tgttggtgat cagctcatta agatattgct cgcctggata ctgtttctta tcggttatag
   106621 tggtcatatc actctccttt tccctgaagc atagcagcgc ggcaggcatt ccagcctctc
   106681 acctctgcaa tagcggcaac agcatcaacc gcgtacatgc taagaggatt aggcattggt
   106741 ttttcttccg gtactactgg aacgggtgga gcggcgtaga cctcaataat tccattatca
   106801 ataggccatt ctccatcctt gaggtagtca cttgtgccgt caacttgctg ttctgcaatg
   106861 tggaatgcac ctattggttt tgcttccagc gatgccagag caattcgtgc cagttcttcc
   106921 gcttcttctg ctggcagtac aacgttgcta ccaggtccgt atgtttcgcg ccactgtttg
   106981 attgtcagta gtcgctcttt ggtaatagtg gtcatgtgtt actccttaac ccgcagtgct
   107041 ttcaactgat gaggggaaca aaatcctttc atcaaatccg gcattcatat catggacagc
   107101 aacacaccaa tccatcgacg aacgattatc aagagcctcc atgatttcat ccatgcggcg
   107161 caggtcatac aggtaaatgc ttttatcgcc aatggtgtaa aaaccaattt ttttcggtga
   107221 tgggcagcga tcaagaacgt cctgtaattc gttcaaccat gcccgttctt tttttgttaa
   107281 agttgccata tcactctcct ttgatgcgaa tgcctgctgc aatgctgttt atgatgctgt
   107341 cagtgcatgg agtagaaagc tgggcatctc cagcaatttt catgacctca acatccgcat
   107401 atcgaatacc gaggtgtatc agaccggcta tacctgactt aagtcgaaca ttttccataa
   107461 acagatcttt ttcccgctgt ttagcggctt ccaactccat cgacaatttt tccaactgct
   107521 ctttatgctt cttgtattcc tgatacgcgt gccaagactg acctttgcgc acactatcag
   107581 tgatatcagc aacctgctcc ggtgttagcg tggtcagtgg ctgtgctgga aaaatcatca
   107641 ctttcccggc atcccaatca aaacccgcct gaattgactg aacctcaact gatggtgttg
   107701 aaccgatgcg accaggcgaa tgaacaacga tcgttacatc catatcgcga cgatggctgt
   107761 ggttgttgga caaaatacga ttcaccaact cagaaaattt ggaaaatttc atgctgatcc
   107821 ctctttctct ccccggataa ttctgttgca cacatccaca cactcgtcgc agatgaaaac
   107881 ggcgtcccca gcaattaact ttttgacggc gtactgggat ttgttgcaga agctgcaaca
   107941 aagtgttcgc ctgactgata aggatggagc gccagatgtc agccgctcca tatcgttctt
   108001 gcgacgcaat atcacacggc tgaactcaac cagtttctcc ggggatatct cctcctccgt
   108061 agcaagcgcc tctaatcgtt cgagtatgtg gaaggctttt
//
LOCUS       DAETQN010000002        88081 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.002, whole genome shotgun sequence.
ACCESSION   DAETQN010000002 DAETQN010000000
VERSION     DAETQN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 88081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88081
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.002"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..905)
                     /gene="nac"
                     /locus_tag="K0W68_000125"
     CDS             complement(<1..905)
                     /gene="nac"
                     /locus_tag="K0W68_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBI3071815.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1232..1307
                     /locus_tag="K0W68_000126"
     tRNA            1232..1307
                     /locus_tag="K0W68_000126"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1265..1267,aa:Asn,seq:gtt)
     gene            complement(1363..2299)
                     /gene="ldtA"
                     /locus_tag="K0W68_000127"
                     /pseudo
     CDS             complement(1363..2299)
                     /gene="ldtA"
                     /locus_tag="K0W68_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(2361..3440)
                     /gene="cobT"
                     /locus_tag="K0W68_000128"
     CDS             complement(2361..3440)
                     /gene="cobT"
                     /locus_tag="K0W68_000128"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBI3071816.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3452..4195)
                     /gene="cobS"
                     /locus_tag="K0W68_000129"
     CDS             complement(3452..4195)
                     /gene="cobS"
                     /locus_tag="K0W68_000129"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBI3071817.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4192..4737)
                     /gene="cobU"
                     /locus_tag="K0W68_000130"
     CDS             complement(4192..4737)
                     /gene="cobU"
                     /locus_tag="K0W68_000130"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBI3071818.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4871..5308)
                     /locus_tag="K0W68_000131"
     CDS             complement(4871..5308)
                     /locus_tag="K0W68_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBI3071819.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5526..5701)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5790..6466)
                     /locus_tag="K0W68_000132"
                     /pseudo
     CDS             complement(5790..6466)
                     /locus_tag="K0W68_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6669..7478)
                     /gene="pduF"
                     /locus_tag="K0W68_000133"
     CDS             complement(6669..7478)
                     /gene="pduF"
                     /locus_tag="K0W68_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HBI3071820.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7990..8274
                     /gene="pduA"
                     /locus_tag="K0W68_000134"
     CDS             7990..8274
                     /gene="pduA"
                     /locus_tag="K0W68_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HBI3071821.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8271..9083
                     /gene="pduB"
                     /locus_tag="K0W68_000135"
     CDS             8271..9083
                     /gene="pduB"
                     /locus_tag="K0W68_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HBI3071822.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            9102..10766
                     /gene="pduC"
                     /locus_tag="K0W68_000136"
     CDS             9102..10766
                     /gene="pduC"
                     /locus_tag="K0W68_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HBI3071823.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10776..11444
                     /locus_tag="K0W68_000137"
     CDS             10776..11444
                     /locus_tag="K0W68_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HBI3071824.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11459..11977
                     /gene="pduE"
                     /locus_tag="K0W68_000138"
     CDS             11459..11977
                     /gene="pduE"
                     /locus_tag="K0W68_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HBI3071825.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11987..13819
                     /locus_tag="K0W68_000139"
     CDS             11987..13819
                     /locus_tag="K0W68_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HBI3071826.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13809..14159
                     /locus_tag="K0W68_000140"
     CDS             13809..14159
                     /locus_tag="K0W68_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HBI3071827.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14179..14454
                     /gene="pduJ"
                     /locus_tag="K0W68_000141"
     CDS             14179..14454
                     /gene="pduJ"
                     /locus_tag="K0W68_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HBI3071828.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14478..14900
                     /locus_tag="K0W68_000142"
     CDS             14478..14900
                     /locus_tag="K0W68_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBI3071829.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14903..15535
                     /locus_tag="K0W68_000143"
     CDS             14903..15535
                     /locus_tag="K0W68_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBI3071830.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15532..16023
                     /gene="pduM"
                     /locus_tag="K0W68_000144"
     CDS             15532..16023
                     /gene="pduM"
                     /locus_tag="K0W68_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HBI3071831.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16027..16302
                     /gene="pduN"
                     /locus_tag="K0W68_000145"
     CDS             16027..16302
                     /gene="pduN"
                     /locus_tag="K0W68_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HBI3071832.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16314..17321
                     /gene="pduO"
                     /locus_tag="K0W68_000146"
     CDS             16314..17321
                     /gene="pduO"
                     /locus_tag="K0W68_000146"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HBI3071833.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            17318..18703
                     /gene="pduP"
                     /locus_tag="K0W68_000147"
                     /pseudo
     CDS             17318..18703
                     /gene="pduP"
                     /locus_tag="K0W68_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18714..19826
                     /locus_tag="K0W68_000148"
     CDS             18714..19826
                     /locus_tag="K0W68_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBI3071834.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            19823..21168
                     /locus_tag="K0W68_000149"
                     /pseudo
     CDS             19823..21168
                     /locus_tag="K0W68_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
     gene            21171..21725
                     /gene="pduT"
                     /locus_tag="K0W68_000150"
     CDS             21171..21725
                     /gene="pduT"
                     /locus_tag="K0W68_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HBI3071835.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21725..22075
                     /gene="pduU"
                     /locus_tag="K0W68_000151"
     CDS             21725..22075
                     /gene="pduU"
                     /locus_tag="K0W68_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HBI3071836.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22080..22523
                     /locus_tag="K0W68_000152"
     CDS             22080..22523
                     /locus_tag="K0W68_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBI3071837.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22624..22953)
                     /locus_tag="K0W68_000153"
     CDS             complement(22624..22953)
                     /locus_tag="K0W68_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBI3071838.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23125..24183)
                     /locus_tag="K0W68_000154"
     CDS             complement(23125..24183)
                     /locus_tag="K0W68_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBI3071839.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24381..24854)
                     /gene="sbmC"
                     /locus_tag="K0W68_000155"
     CDS             complement(24381..24854)
                     /gene="sbmC"
                     /locus_tag="K0W68_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HBI3071840.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(24973..26139)
                     /gene="dacD"
                     /locus_tag="K0W68_000156"
     CDS             complement(24973..26139)
                     /gene="dacD"
                     /locus_tag="K0W68_000156"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HBI3071841.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26348..27775
                     /gene="sbcB"
                     /locus_tag="K0W68_000157"
     CDS             26348..27775
                     /gene="sbcB"
                     /locus_tag="K0W68_000157"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBI3071842.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27818..28045)
                     /locus_tag="K0W68_000158"
     CDS             complement(27818..28045)
                     /locus_tag="K0W68_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBI3071843.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28059..29117)
                     /gene="yeeE"
                     /locus_tag="K0W68_000159"
     CDS             complement(28059..29117)
                     /gene="yeeE"
                     /locus_tag="K0W68_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBI3071844.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(29296..30654)
                     /gene="plaP"
                     /locus_tag="K0W68_000160"
     CDS             complement(29296..30654)
                     /gene="plaP"
                     /locus_tag="K0W68_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBI3071845.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(30644..30706)
                     /gene="yoeI"
                     /locus_tag="K0W68_000161"
     CDS             complement(30644..30706)
                     /gene="yoeI"
                     /locus_tag="K0W68_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBI3071846.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(30921..31850)
                     /locus_tag="K0W68_000162"
     CDS             complement(30921..31850)
                     /locus_tag="K0W68_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3071847.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(31896..32720)
                     /locus_tag="K0W68_000163"
     CDS             complement(31896..32720)
                     /locus_tag="K0W68_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI3071848.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(32803..33057)
                     /gene="yoeB"
                     /locus_tag="K0W68_000164"
     CDS             complement(32803..33057)
                     /gene="yoeB"
                     /locus_tag="K0W68_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBI3071849.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(33054..33305)
                     /gene="yefM"
                     /locus_tag="K0W68_000165"
     CDS             complement(33054..33305)
                     /gene="yefM"
                     /locus_tag="K0W68_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBI3071850.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            33588..33638
                     /gene="hisL"
                     /locus_tag="K0W68_000166"
     CDS             33588..33638
                     /gene="hisL"
                     /locus_tag="K0W68_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBI3071851.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    33614..33737
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            33784..34683
                     /gene="hisG"
                     /locus_tag="K0W68_000167"
     CDS             33784..34683
                     /gene="hisG"
                     /locus_tag="K0W68_000167"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBI3071852.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            34689..35993
                     /gene="hisD"
                     /locus_tag="K0W68_000168"
     CDS             34689..35993
                     /gene="hisD"
                     /locus_tag="K0W68_000168"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBI3071853.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            35990..37060
                     /gene="hisC"
                     /locus_tag="K0W68_000169"
     CDS             35990..37060
                     /gene="hisC"
                     /locus_tag="K0W68_000169"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBI3071854.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCESGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            37060..38127
                     /gene="hisB"
                     /locus_tag="K0W68_000170"
     CDS             37060..38127
                     /gene="hisB"
                     /locus_tag="K0W68_000170"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBI3071855.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            38127..38717
                     /gene="hisH"
                     /locus_tag="K0W68_000171"
     CDS             38127..38717
                     /gene="hisH"
                     /locus_tag="K0W68_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBI3071856.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            38717..39454
                     /gene="hisA"
                     /locus_tag="K0W68_000172"
     CDS             38717..39454
                     /gene="hisA"
                     /locus_tag="K0W68_000172"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBI3071857.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            39436..40212
                     /gene="hisF"
                     /locus_tag="K0W68_000173"
     CDS             39436..40212
                     /gene="hisF"
                     /locus_tag="K0W68_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBI3071858.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            40206..40817
                     /locus_tag="K0W68_000174"
     CDS             40206..40817
                     /locus_tag="K0W68_000174"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBI3071859.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(40914..41891)
                     /gene="wzzB"
                     /locus_tag="K0W68_000175"
     CDS             complement(40914..41891)
                     /gene="wzzB"
                     /locus_tag="K0W68_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBI3071860.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLAQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(42037..43203)
                     /gene="ugd"
                     /locus_tag="K0W68_000176"
     CDS             complement(42037..43203)
                     /gene="ugd"
                     /locus_tag="K0W68_000176"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HBI3071861.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(43453..44859)
                     /gene="gndA"
                     /locus_tag="K0W68_000177"
     CDS             complement(43453..44859)
                     /gene="gndA"
                     /locus_tag="K0W68_000177"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBI3071862.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(45023..46393)
                     /gene="cpsG"
                     /locus_tag="K0W68_000178"
                     /gene_synonym="manB"
     CDS             complement(45023..46393)
                     /gene="cpsG"
                     /locus_tag="K0W68_000178"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HBI3071863.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(46498..47934)
                     /gene="cpsB"
                     /locus_tag="K0W68_000179"
     CDS             complement(46498..47934)
                     /gene="cpsB"
                     /locus_tag="K0W68_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBI3071864.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(47937..49160)
                     /gene="wcaI"
                     /locus_tag="K0W68_000180"
     CDS             complement(47937..49160)
                     /gene="wcaI"
                     /locus_tag="K0W68_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBI3071865.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(49157..49636)
                     /gene="gmm"
                     /locus_tag="K0W68_000181"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(49157..49636)
                     /gene="gmm"
                     /locus_tag="K0W68_000181"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBI3071866.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(49639..50604)
                     /gene="fcl"
                     /locus_tag="K0W68_000182"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(49639..50604)
                     /gene="fcl"
                     /locus_tag="K0W68_000182"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBI3071867.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(50607..51728)
                     /gene="gmd"
                     /locus_tag="K0W68_000183"
     CDS             complement(50607..51728)
                     /gene="gmd"
                     /locus_tag="K0W68_000183"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBI3071868.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(51754..52302)
                     /gene="wcaF"
                     /locus_tag="K0W68_000184"
     CDS             complement(51754..52302)
                     /gene="wcaF"
                     /locus_tag="K0W68_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBI3071869.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(52318..53064)
                     /gene="wcaE"
                     /locus_tag="K0W68_000185"
     CDS             complement(52318..53064)
                     /gene="wcaE"
                     /locus_tag="K0W68_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBI3071870.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(53075..54292)
                     /gene="wcaD"
                     /locus_tag="K0W68_000186"
     CDS             complement(53075..54292)
                     /gene="wcaD"
                     /locus_tag="K0W68_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBI3071871.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(54267..55484)
                     /gene="wcaC"
                     /locus_tag="K0W68_000187"
     CDS             complement(54267..55484)
                     /gene="wcaC"
                     /locus_tag="K0W68_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBI3071872.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(55481..55969)
                     /gene="wcaB"
                     /locus_tag="K0W68_000188"
     CDS             complement(55481..55969)
                     /gene="wcaB"
                     /locus_tag="K0W68_000188"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBI3071873.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(55972..56811)
                     /gene="wcaA"
                     /locus_tag="K0W68_000189"
     CDS             complement(55972..56811)
                     /gene="wcaA"
                     /locus_tag="K0W68_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HBI3071874.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(56904..59066)
                     /gene="wzc"
                     /locus_tag="K0W68_000190"
     CDS             complement(56904..59066)
                     /gene="wzc"
                     /locus_tag="K0W68_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBI3071875.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(59069..59512)
                     /gene="wzb"
                     /locus_tag="K0W68_000191"
     CDS             complement(59069..59512)
                     /gene="wzb"
                     /locus_tag="K0W68_000191"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBI3071876.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(59518..60657)
                     /gene="wza"
                     /locus_tag="K0W68_000192"
     CDS             complement(59518..60657)
                     /gene="wza"
                     /locus_tag="K0W68_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HBI3071877.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            60966..61115
                     /locus_tag="K0W68_000193"
     CDS             60966..61115
                     /locus_tag="K0W68_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071878.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            61316..62899
                     /locus_tag="K0W68_000194"
     CDS             61316..62899
                     /locus_tag="K0W68_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBI3071879.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(63173..65026)
                     /gene="asmA"
                     /locus_tag="K0W68_000195"
     CDS             complement(63173..65026)
                     /gene="asmA"
                     /locus_tag="K0W68_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBI3071880.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(65048..65629)
                     /gene="dcd"
                     /locus_tag="K0W68_000196"
     CDS             complement(65048..65629)
                     /gene="dcd"
                     /locus_tag="K0W68_000196"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBI3071881.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(65721..66362)
                     /gene="udk"
                     /locus_tag="K0W68_000197"
     CDS             complement(65721..66362)
                     /gene="udk"
                     /locus_tag="K0W68_000197"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBI3071882.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            66680..69997
                     /locus_tag="K0W68_000198"
     CDS             66680..69997
                     /locus_tag="K0W68_000198"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBI3071883.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(70107..70955)
                     /gene="alkA"
                     /locus_tag="K0W68_000199"
     CDS             complement(70107..70955)
                     /gene="alkA"
                     /locus_tag="K0W68_000199"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBI3071884.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            71089..72441
                     /gene="yegD"
                     /locus_tag="K0W68_000200"
     CDS             71089..72441
                     /gene="yegD"
                     /locus_tag="K0W68_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBI3071885.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(72454..74394)
                     /gene="yegI"
                     /locus_tag="K0W68_000201"
     CDS             complement(72454..74394)
                     /gene="yegI"
                     /locus_tag="K0W68_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HBI3071886.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(74391..75152)
                     /gene="pphC"
                     /locus_tag="K0W68_000202"
     CDS             complement(74391..75152)
                     /gene="pphC"
                     /locus_tag="K0W68_000202"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HBI3071887.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(75149..75808)
                     /locus_tag="K0W68_000203"
     CDS             complement(75149..75808)
                     /locus_tag="K0W68_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBI3071888.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(76029..76088)
                     /gene="ibsA"
                     /locus_tag="K0W68_000204"
     CDS             complement(76029..76088)
                     /gene="ibsA"
                     /locus_tag="K0W68_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HBI3071889.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(76361..76417)
                     /locus_tag="K0W68_000205"
     CDS             complement(76361..76417)
                     /locus_tag="K0W68_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBI3071890.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(76688..76744)
                     /locus_tag="K0W68_000206"
     CDS             complement(76688..76744)
                     /locus_tag="K0W68_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBI3071891.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            77023..78264
                     /gene="mdtA"
                     /locus_tag="K0W68_000207"
     CDS             77023..78264
                     /gene="mdtA"
                     /locus_tag="K0W68_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HBI3071892.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            78264..81386
                     /gene="mdtB"
                     /locus_tag="K0W68_000208"
     CDS             78264..81386
                     /gene="mdtB"
                     /locus_tag="K0W68_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HBI3071893.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            81387..84464
                     /gene="mdtC"
                     /locus_tag="K0W68_000209"
     CDS             81387..84464
                     /gene="mdtC"
                     /locus_tag="K0W68_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBI3071894.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            84465..85880
                     /locus_tag="K0W68_000210"
     CDS             84465..85880
                     /locus_tag="K0W68_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3071895.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            85877..87280
                     /gene="baeS"
                     /locus_tag="K0W68_000211"
     CDS             85877..87280
                     /gene="baeS"
                     /locus_tag="K0W68_000211"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBI3071896.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            87277..87999
                     /gene="baeR"
                     /locus_tag="K0W68_000212"
     CDS             87277..87999
                     /gene="baeR"
                     /locus_tag="K0W68_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBI3071897.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        21561 a        23315 c        22149 g        21056 t
ORIGIN      
        1 tgccactgcc ttttttccat cactggggaa ctaatcactg acattaacaa ctctttcact
       61 gcctgtgcct gtggcgataa gttcgctctg gcgggtaaat ttaatgacaa agagagactc
      121 atggaaggag tggtaatgcg tgacatccac ccatttactg cgccacataa cgaacgcgcg
      181 gccgattcgg gtaatactgc aacgcccatg ccgctggcaa tcgctgcggt aagcgtggca
      241 atagactcaa tttcaccaat gacttttgcc gtgaatcgcc gtaaggaaaa agcctcatca
      301 acacgaagtc taatagcact gtaatcactg gggagaaaga ggttcatttt cgcaatagca
      361 ttcacatcaa cgctttgccc cgggcaatct tgagttccta ccagaaaaag atcttctttc
      421 agcaaagcct gactggatac accagccaca ggggaatgct cataaatcac cgccatatcg
      481 agttggtgat ttatcaattt ttcgttaagc actgcaccac tattttcatg aagatagata
      541 acgatctccg gaaattcagc gcgaaccgcc tgtaataagg gcatggtgat ggatgacgca
      601 gcggttcctg gtgcaaagcc aatcgagact tgccccgata atgcctgacc aacgttatgc
      661 accgccagtt gggcctgttc acactgacgt aaaatggccc gcgcatgggt atagagaatt
      721 tttccggcgt ctgttggcgt aacgccccgc tttgtacgga tcaaaagttg ttgatttaac
      781 tcaccttcca gtgtggcaac ctgctggctg agcgctggtt gtgcgatatg caatacttca
      841 gcagcctggg tcaggctacc aatatctaca atttttacga agtatttcag gcgtctgaag
      901 ttcatgttgc ctccggtttt taagaatcgg cccaagtgcc gccattactt acaaccagat
      961 tgcaagatgc ttgccagttt tattttggcg ttgatgtaca agctaaccaa ctgtcaaata
     1021 agagattatg atagattcgt catttgctcc tttaatcagc tgtcgcgttc ccctgcccta
     1081 taaaaggagg gtatgcacca cgatggttca ttacccaata agattgaaag ctcaccactt
     1141 tgttgaaatt gacagcaaac aaacaaaaaa atgcatttca ccctttgaca tcaccatgca
     1201 ctgccattaa tatgcgcccc gttcacacga ttcctctgta gttcagtcgg tagaacggcg
     1261 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat tcaaaaaagc
     1321 ctgctttcta gcaggctttt tgctttctaa ttaccaacgc tcttaaaaca tctgtcttga
     1381 accagaacta atttgcacag gcattcccga tcgacgttgc aacgcagcat ttgcgcgatt
     1441 tacatcaact tcttgcccgc tgataaacgc ccgcaaagat ggggttaccg gcaatggcac
     1501 ttttcggtca gactcatatt ctgcacgatt gcgcgacaac ggctcatgaa cttccagcca
     1561 gttcgagcca tctggttcag tggtgtattt tactggctgg tcgataattt gcacacgcgt
     1621 cccaacagga acattatcaa acagatattt gatatcgtca ttgcgcagac gaatacagcc
     1681 ctgacttacc cggagcccaa taccaaaatt ggcattggta ccatggatgg catacaacct
     1741 gccaatataa atcgcgtaca gccccatggg attatcgggg cccgcaggaa caaatgcggg
     1801 caaactctcc cctcgtttcg catattcgcg ccgagtgttc ggcgttggcg tccaggttgg
     1861 agcttcttgt ttacgttcaa cggtagtcac ccagttacgc ggggtttctc gcccagcctg
     1921 gccgatacca ataggaaaga cttccacagt attactgtct ggtgggtagt aataaagacg
     1981 catctcagcg acgttaacca caatcccttt acgaacagtg gcgggcaaaa tcagttgctg
     2041 cggaatggtg agttgcgagc cagacttcgg caaaaaaaac atcagcgccc gggttcgctt
     2101 ccagcatgtt acttaaccct tgcccgtatt gtgcggcaaa agtctccagc ggctgggtat
     2161 tgtgatcagg aacagttaca gtaagcgact gccccactaa acggctaccc tctggaggta
     2221 atggataagt taccgccagg ctattatggc tggcaaaaag cagagcaaat gagcaaaaaa
     2281 tatttacacg acgcatcatg tccctttcct aagtcgcgaa agctatccgt taagtatagc
     2341 ttttatcaga cttttcgttt ttaactgttc aaatcagaag tcgtattccc cggtagaaca
     2401 atattactgg cagcaagttc gcccatgttg ttatatatcg cacaggcagc ttcgatgatg
     2461 ggcatcgcca gagctgcgcc actaccctca cctaaacgca tctccatatt gagataaggc
     2521 tccagcccca aatgcgacag cgcaatacgc gccccttttt ctgccgacag gtgagaagga
     2581 atgagatacg gtttgattgc aggagacatc tggcaggctg cgagcgcggc agcataagaa
     2641 agaaatccat ccagcaacac gggtaaaccg caggaagcag cgcctaacat cacgccagcc
     2701 attccgacca aatcaaatcc acccactttt gccaggacat caacgccatc ctgcggattt
     2761 ggttgattca acgtaatcgc ccggcgcaca acatcaattt tattagccag tttatctgtc
     2821 ggcaggtttg cgccaatccc aaccacttct tcaggagccc ggccagtgat tgtgctgact
     2881 attgccgctg ccggtgtcgt gtttgccatc cccaggtcac ctacaccaaa cagcgtaaca
     2941 ccgttttttg ccagctcccg cgtataacat atgacgtcca aaagcaactt ttctgccaga
     3001 tggcgactca ttgccggagc tgaagcaata ttgccactac ctcgagcaac acgcatgttg
     3061 ataagcccgg ggataggctc agcagtatca ataccaacat caattacgta gacgttagcg
     3121 cccgcttgtg ctgccagcac acacacgccg gttgttccgc gggtcatatt ttcagcctga
     3181 atggctgtca cttcttttgg ggaaatagcg accccttcct cccagacgcc gtgatcggca
     3241 cacatgacca gtaccgcttt tttgcccaca tgcggaacgc cattcaaccc cggcattcct
     3301 gccagttgta tggcaagcgc ctccagcctt cccaggctac caacaggttt gagtaacccg
     3361 tcgatatgcc gttgtgcacg cgacatagcg gcaggatcaa tagcagggat cgtattcagt
     3421 aaatcggcaa gtgtttgcat gtcacgtctc tttatagcag tgccagcagg aagaccagtt
     3481 caccaagctc tatagcagct cccagcgtat cgccggtttg cccgcccagc gtacgtttga
     3541 gaagctgacc gaggatgaaa atcgccacca tcgtcaccac catagcagcc acaccatgca
     3601 tacccggcaa taatacggca gcaaaaatag cggctaagcc gagtgtgacg caggtttgtc
     3661 gcccatcaat tttgccaatg aacacattgc caagtccttc ttcccgggca taacgatgac
     3721 gatacatcaa taatgcggca gtcccgcggc tgaccgcaca tgctgccgcc agcgatgcaa
     3781 ggatcggttc gccacgcaga gccaactcgc ttaataccag aatctttgcc agtacaacaa
     3841 aaatcaatgc cagaccgccg tgggtgccta aacggctatc acgcatgatt tccaacatgc
     3901 gatcgcggct acgtgcagaa aatacgccat cacaggtatc ggcgaggccg tccaggtgga
     3961 atcccccggt catcagcgcc agcacaagta cgctaaacag tgccgccagt ggtacgccac
     4021 accatgcctg cagcaccatg aagaccagcc cgctaatcgc gccaagcaat aatccaatta
     4081 aaggaaaagt aataataccg cgagaataat gctcgaaatc cagtccctgg gaccagcgac
     4141 gcggtacagg cagacgcgta ataaaagaga gcatcgccca aaataattta ctcatttgat
     4201 ttttactcca atacccgaaa ccaccaacca tacttcattt gccgcagcgg ccagctgctg
     4261 atttacccgc ccggcaatat cacgaaaatg tcgtgccaga cgactctccg gcacaatccc
     4321 cattcccact tcgttagtca ctaatacaac ctttgcggga caacgttggc aggcagcaat
     4381 caacgactga atctcagcat taatcgcctg ttccatcgcc tgataatccc attcatcagg
     4441 gtctttatcg ccgccataat caaacaacag attagtcacc atggtggtaa cgcattccag
     4501 caatacagcc tcatgcgggt taatgtctgc atgaattaat tcatcaagat gttgccagcg
     4561 ctccactgtg tgccagtgcg ccgggcggcc ttgccgatga tgttctatcc gtgcagccat
     4621 ctcatcatca aggatttgtg aggtggcgat atacagaacc tgcgaagagt ccccaataag
     4681 cgcctctgca tggcgactct tcccgctccg tgcgcccccc gtcaccaaaa tcatcatacc
     4741 gactcctgat tcaattgtat gatggtattc attgaacaga acaatggatc ccccaccgtt
     4801 gctgaccgca aaacatttac gcgattaata ctgcgcgtaa tataattaaa aatccagcat
     4861 tctcaatcca ttacgaaaga agatggcatt gcggaatagc cagacttcct cgtgttaacg
     4921 tccgacaaaa tgcgtcattg acagcggttt ctctggaatg acagatttca ttcagataat
     4981 ggattaatgc tacagcccca ccagccggac cagtcagcaa acgggcattg tgttctgcaa
     5041 tcaaaattac cgtcgttccc tcagaatgag cgagccattc tggtacggat atctcgcttt
     5101 cagctgctat caatagcagc actgaataat cgctatacgc atctgataac gattgcacag
     5161 gcccaccatc aagaggctgg aaatcttgct gccagtcctc accaacaaaa caggttattg
     5221 gcaatatttc agccagatgt ttcgccagat ccacatctcc agataagagg caaaaaagag
     5281 ctatcgattc gggttttatc gttttcatgc ctctcgccct ccacttaatt tcctgtaatg
     5341 gcggtctggt tgataagcca tgaaacaata ttgccacacc ttgaatgcag ccctggtgcc
     5401 tgtcggcact gcgatgaaag aactaaatga tgccgccata ccctgatcct ttcagggtgg
     5461 taggggtata aagacgcgcc agtactctac cggacgcttc cgggcaaccc acttcccacc
     5521 gaagttgttg gtattgactg acagactggt cttctggctt agcgtcatcc tcacccgacc
     5581 ttcccaatct cgcgatcagt ggcattttcg ggtttgtcag cattacagca gcgggggctg
     5641 cgagggattt tcaccctctt cccaggcaca aatgtgccac acctgtcagc gtatgttatg
     5701 agctgatttc acttttccca taacacgtaa ataacgttaa ttattcattc cagcgtttat
     5761 ttaaaccttc ccttttttta gcatttattc tataccgttt ccatcgccgc attttcactc
     5821 acttgctgtc gatacacctg cggcgtaacc tgataagtct gacggaaaac tttgcaaaaa
     5881 taactgtatt gtcgtgggga ttaggcgcac gagtctggcg caccgaatct gaccgagatt
     5941 tgttctcttt aataaccaca aaattgagtt gttttttgag gcaattttcc acaatcaact
     6001 tcaacaaatc agcagaggcc ataactcttg agtaatccat ctcaggaact ttgcggaatt
     6061 cattaagtaa ttcgggatct gcctgccagc aatcatcgac attcagaata tcgaccaaat
     6121 cgacatcatc attacgcaga cgaacttgcc cgcacaatac aaaaccgact aaatgtccgg
     6181 cgattaccag tggaatagag aaatcggtta atccggcatg acagcgataa atacaaggct
     6241 gatcggattt agaggcttcc aggcccccac aacgatcgct catgcgacat cgagtgctta
     6301 gctgagggtg ttgccgcatc atctgacaaa atggagtgaa gttaaacagc tcggaaattt
     6361 catcgccgtg aatattgacc acgacaaccg caaggctggt cgcctgcgca aaatcctgcg
     6421 cgattttatt aatgagttct gaattaagtg tcgccgcaga aatcatgata aaacctccca
     6481 gttaatttat tgttatagca aaacaattgt tttacattta atcttcacat catccctgat
     6541 gatttactga cgagaagcac aaaggacaat aacaaaagcc tccgtgaagc gggagacttt
     6601 ttaataagtt cggaaagcag atcacaaaca gtaaaaaaat aaatgtttat aactgccaga
     6661 gtatttcgtt attcgctaat tcctttaggt gtaacactat tgacaggtaa attctttgca
     6721 atgagatagc gataaatcgc cgcaccaaca catgccccaa tgatcggtgc aataatgggt
     6781 acaatgaaat aaggaatatt gcgtccacca gtcatcgcaa tttctcccca ccccgcaagc
     6841 caggtgaaaa tcttaggtcc aaaatcacgc gctggattca tggcaaaacc ggttaatggt
     6901 ccggtagaag caccaataac agcaaccaga ataccaataa gtaaaggtgc aagcggtcct
     6961 ttgggtacgc cattaccgtc atcggtcaac gccataatca accccattaa catggacgtt
     7021 atgaccactt cgaccaacgc agcttgccat acattaatcg ccgctgccgg ataagtactg
     7081 aagatactgg cgagttgtaa actttctgcg ctaccacgca ccatattgtg agcagattca
     7141 aaatcggtaa acagactact gtatagagaa tatgccagaa ctgcaccacc aaaggcaccc
     7201 gctaattgcg ccagacaaaa tggcagcact ttacgggctg ggaaagaggc aaacagccac
     7261 agagcaatgg taactgccgg gttgagatga gcgccggaaa tacctgccgt aaggtaaaca
     7321 gcaagcgaaa tccctaaccc ccaaatgata caaatttccc acagtcccag acttgccccg
     7381 gcaactttca gggcgcacaa acaaccaatg ccaaaaaaga ggaacagtcc ggtgcccaaa
     7441 aactcggcaa cgcattgcgc cttgagtgaa tcattcatag tgacaccttt tttacggagt
     7501 ttttctttat cagccaacta tgcgctgact gttgtatata ccgagcacaa ctataaaaaa
     7561 ttgctctgca gaaaatattg ccaacatgca tgacagactc gtgtaaaaat cgtgctaaca
     7621 taacaaatta ttgtcattac cgaatcatgc aacacagcgt tagtcagtgt tgaaagtaat
     7681 aattcccacc aaacttttac aataaaaaat tgccatcatg acataataaa attgaaacaa
     7741 atatcacagt tccttcttcg aactaaactc aaccaaatat tactcgcgat taaactcact
     7801 agccaacgca attaattcac attgaaatat ccacaaaaca accaataaat taacaacata
     7861 tagttcataa aagcaatttt ataatatttg ttctctatta atttctctca ttagccgatt
     7921 aagtcagcca ttctttttcc tcgctttttt ttatagtcat atcgtcaaaa aaatccaggc
     7981 gaggtcttta tgcaacaaga agcgttagga atggtagaaa ccaaaggctt aactgccgcc
     8041 atagaggccg cagacgccat ggtgaagtcg gcaaatgttt tgctggtcgg ctacgaaaaa
     8101 ataggttccg ggctggtgac cgtcatcgtt cgtggtgatg tcggggcggt aaaagcggca
     8161 acagacgcgg gtgccgcagc tgcgcataac gtcggcgaag taaaagccgt acacgttatt
     8221 ccccgcccac ataccgatgt cgaaaaaatc ttaccaaagg gaattaaccc atgagcagca
     8281 atgaactggt cgaacagatc atggcgcagg tcatcgctcg cgtagcgacg ccagagcatg
     8341 aggttctccc gaaaaccact catcaaatac gagagacggc tatggcagaa aaaagctgca
     8401 cgctaacaga atttgttggc accgctattg gcgacactgt aggtctggtc attgcgaacg
     8461 ttgatagcgc cctgttggac gcgatgaaac ttgaaaagcg ctatcgttcc attggcattc
     8521 tgggtgcgcg tattggcgca gggccacaca ttatggccgc tgatgaagcg gtaaaagcca
     8581 ccaacactga agttattagc attgaacttc ctcgcgatac taaaggcggt gccggacacg
     8641 gctcactgat cctcttaggt ggcaacgatg tttccgacgt gaaacgcggt attgaagtcg
     8701 ccctgaaaga actcgatcgg acctttggcg atgtctatgc caacgaagcc ggacatcttg
     8761 aactgcaata taccgcccgc gccagctatg cactggaaaa agcgtttggt gcaccactgg
     8821 gacgggcttg cggcgttatt gtcggcgctc cggctgctgt gggtgttctg atggccgaca
     8881 ccgcgctgaa gtccgccaat gtggatgttg tggcatacag ctccccagct caagacacca
     8941 gctacagcaa tgaagtcatt ctgattatct cgggtgactc cggcgcagtc cgccaggcgg
     9001 tcatctccgc acgcgaaatc ggtaaaaccg tgctttcgac tctcggcgat acaccgaaaa
     9061 acgatcaccc ttcctacatc tgaattctct gaggcagatt tatgagatcg aaaagatttg
     9121 aagcactggc aaaacgcccg gtcaaccagg acggtttcgt taaagagtgg attgaagaag
     9181 gctttattgc gatggaaagc ccgaacgatc ctaaaccgtc aatcaaaatt gttaacggcg
     9241 tcgttaccga actggatgga aaacccgcca gccagtttga cctgatcgac cactttatcg
     9301 cccgctacgg catcaatctc gcgcgcgccg aagaagtcat cgcgatggat tcggtaaaac
     9361 ttgccaatat gttgtgcgat ccaaatgtca aacgtagcga tatcgttccc cttaccacag
     9421 caatgacgcc tgcaaaaatc gtcgaagtgg tttcgcagat gaacgtggta gagatgatga
     9481 tggcgatgca gaaaatgcgc gcccgtcgaa caccatcaca acaggctcac gtcaccaacg
     9541 tcaaagataa cccagtacaa attgccgccg acgcagctga aggcgcatgg cgcgggtttg
     9601 atgaacagga aaccaccgtt gccgttgctc gctatgcacc gttcaacgcc atcgccctgc
     9661 tggtcggctc acaggtggga cgtccgggcg tcttgaccca gtgttcactg gaagaagcca
     9721 ccgaactgaa actcggtatg ttaggccaca cctgttacgc cgaaacaatt tctgtctatg
     9781 gtacagaacc tgttttcacc aatggtgatg ataccccgtg gtcaaaaggg tttctcgcgt
     9841 catcctatgc ttctcgtggc ttaaaaatgc gctttacttc cggctccggc tctgaagtac
     9901 agatgggtta cgcggaaggc aaatcaatgc tttacctcga ggctcgttgc atttacatca
     9961 ccaaagccgc aggcgttcag ggtctgcaga atggttcggt aagctgcatc ggtgttccat
    10021 cagctgtgcc atcaggcatc cgcgcagtat tagcggaaaa cttaatttgc tcatcgctgg
    10081 atctggaatg cgcctccagt aatgaccaga cctttaccca ctcggatatg cgccgtaccg
    10141 cacgtttcct gatgcaattt ctgccaggca ccgactttat ctcttccggt ttttccgctg
    10201 tgccgaacta cgacaacatg tttgctggtt caaacgaaga tgctgaagac ttcgatgatt
    10261 acaacgtcat tcagcgtgac ctgaaggttg atggtgggct gcgaccagta cgcgaagaag
    10321 atgttatcgc tatccgcaat aaagccgctc gcgctttaca ggctgtcttt gccggtatgg
    10381 gactaccgcc tattaccgat gaagaagtcg aagcggcaac ctatgcccac ggttcaaaag
    10441 atatgcctga gcgcaatatt gtggaagaca tcaagttcgc ccaggagatt atcaataaaa
    10501 accggaacag cctggaagtg gtgaaagcct tggcgcaagg cagttttacc gatgtcgccc
    10561 aggacatgct caacatgcaa aaagcaaaac tgaccggcga ttatctccat acctcagcca
    10621 tcatcgtcga tgacggacaa gtgctctctg cggtcaatga cgtcaatgat tacgccggac
    10681 cggctacagg ttaccgcctg caaggggaac gctgggaaga gatcaaaaat attcccggtg
    10741 cacttgatcc caacgaaatt gactaagggt gagaaatgga aattaacgaa aaattactgc
    10801 gtcagattat cgaagacgtg ctggcagaaa tgcaacccag cgacaagtcc gtttcatttc
    10861 gcgcacctgt ctctgcaact gtcccttccg cgccagatac cggaaatttt ctgaccgaaa
    10921 ttggcgaagc ccaacaaggc actcagcaag atgaagtcat tattgctgta ggcccggcgt
    10981 tcggcctggc gcaaaccgtc aatatcatcg gtattccgca taaaaacatc ttgcgcgaag
    11041 tgattgccgg tatcgaagaa gaaggcatta aagcccgtgt aattcgctgc tttaaatcgt
    11101 ccgatgtcgc attcgtagca gtcgaaggcg atcgtcttag tggttcaggt attgctatcg
    11161 gtattcagtc gaaaggcaca acggttatcc accagcaggg acttcctccc ttgtctaacc
    11221 tggaactttt cccacaagcg cctttgttga cgctggatac ttatcgtcag attggcaaaa
    11281 acgccgcgcg ttatgcgaag cgcgagtctc cacaaccggt gccgaccctc aacgatcaaa
    11341 tggcgcggcc gaaataccag gccaaatctg ccattttgca tattaaagag acgaagtacg
    11401 tggtcactgg caaaaaaccg caagaactgc gcgtgacgtt ttaacaaagg aaaccttcat
    11461 gaataccgat gcaattgagt cgatggtcag ggacgtgttg aaccgcatga acagtttaca
    11521 ggatgcagct cctgtttctg cggtccccaa cgcatcaata ctgagcgcca aagtgacgga
    11581 ttacccgctg gcaaataaac atccggaatg ggttaaaaca gcgaccaata aaacgctcga
    11641 cgatttcact ctggaaaatg tgctaagcga taacgtcacc gccctggata tgcgcattac
    11701 ccctgaaaca ttgcgtattc aggctgcgat tgccagagat gctggtcgcg atcgtctggc
    11761 gatgaatttt gaacgcgccg ctgagcttac ctcagtacca gatgatcgca tcctcgaaat
    11821 ctacaatgcg ttacgccctt atcgttcaac taagcaagaa cttattgcca ttgctgacga
    11881 tcttgaacag cgttatcagg ccaaaatttg tgctgcattt gtccgtgaag ctgcggaact
    11941 ttatgtcgag cgcaaaaaac tgaaaggcga cgattaagga cgaactatgc gatatatagc
    12001 aggcattgat atcggcaact catcaacaga ggttgcgctg gcaacgctca gtgcaactgg
    12061 agagttgtcg ttcgtgagta gcgccctgga ggaaaccacc ggaatcaaag gtacgttgcg
    12121 taacgtccat ggtattcagg aggcgctcgc acaggcaacg aaaaaagtcg gcatcaatgt
    12181 tagcgatatt tcgcttattc gtatcaacga agcaactccg gtcattggtg atgtggcgat
    12241 ggaaaccatc actgaaacta tcatcaccga atcaaccatg atcggtcaca acccgaaaac
    12301 tccgggcgga gtggggctgg gcgtagggtt caccatcact ccgcaagagt tattaacacg
    12361 cccggcggac acaccttaca tcctggtggt gtcatcggca tttgattttg ccgatatcgc
    12421 gacgatgata aacgcctccg tacgcgccgg atatcagctg actggcgtta ttctgcaacg
    12481 cgacgatggc gtactggtca ataaccgcct ggaaattcct ttacctattg tggatgaagt
    12541 cctctacatc gatcgtattc cgctcggcat gctggcagca attgaagttg ccgttcccgg
    12601 gaaagttatc gaaaccctgt ctaacccgta tgggattgcc accgtattcg ccctcaatgc
    12661 tgaagagact aaaaatattg ttcctgtcgc gcgggcgcta atcggcaacc gttcagcagt
    12721 ggtagtgaaa acgccctctg gcgacgttaa agcccgttcg atacccgccg gaaatatcga
    12781 actgctttct gccgggcgca caacgcgcgt tgatgtagct gctggtgccg atgccattat
    12841 gaaagcggtc ggtgagtgcc caaaactgga aaacgtgacg ggagaaccag gcaccaacat
    12901 cggcggaatg ctggaacatg ttcgccagac catggcggaa ctgaccaaca aacccagcaa
    12961 cgaaattttt attcaggatt tgctggcgat agatacctct gtccccgtca gcgtaaccgg
    13021 tggtctggca ggtgaatttt cactggaaca ggctgtgggt attgcctcta tggtgaaatc
    13081 cgaccgtctg caaatggcga tgatcgccag tgaaattaag caaaaactgc atgttgatgt
    13141 tcaggtcgga ggcgcagaag cagaagccgc cattcagggg gccttgacta cccctggaac
    13201 cacacgtcca ctggcaattc tggacttagg tgcaggctct accgatgcct ccatcatcaa
    13261 tcagagtggg gaaatcgttg ccactcacct tgcgggagcg ggcgacatgg taacgatgat
    13321 tatcgctcgt gagctgggct taaatgatcg ttatctggca gaagaaatca aaaagtaccc
    13381 gttagcaaaa gtagaaagcc tgttccactt acgccatgaa gacggtagcg ttcagttctt
    13441 ccctactccc ctatcccccc atgtatttgc ccgcgtctgt gtagtgaaac ccgacgaact
    13501 ggtgcctatt cccggtgatt tgacgctgga aaaagtacgc gctgttcgtc gcagtgcgaa
    13561 agagcgtgtc ttcgtaacca atgccctgcg cgcgctacgt caggtaagcc ctgccggaaa
    13621 tattcgcgac attccgttcg tcgttctggt gggtggttct tcgctggatt tcgaagtacc
    13681 gcaattggtc accgacgcgc tggcacatta tcgactggtc gccgggcggg ggaatatccg
    13741 tggtagcgaa ggtccgcgca acgcggtagc tactgggcta ctcatcgcct ggcacaagga
    13801 gtcgattcat ggaaagtaaa gagcgtgcac cagccatcgt tgtcatggag atcggtgact
    13861 gtatcactac atgggatgag gtgcttcttg gcattgaaga ggaaggtatt ccctttcgca
    13921 ttcaacacat tccgtcaggt gaagttatcg atagcgcctg gctggctgct cgtcaatctc
    13981 ccctgttggt tggcattgcc tgcgaccggg aagaactgat tgtgcattac aaaaacttac
    14041 cggcatcagc gccactgttt acgctgacgt atcaacaaga taaccatacc cggcgcagca
    14101 tcggcactaa cgctgcccgc ctggtaaaag gaattccctt ccgggaattc cactcataat
    14161 ccacaggaga aacgctgtat gaacaacgca ctaggactgg ttgaaacaaa agggttagtt
    14221 ggcgcaattg aagctgcaga tgccatggtt aaatccgcca acgtgcagct ggttggctat
    14281 gaaaagattg gttcgggtct gattacggtg atggtccgcg gtgatgttgg cgcagtaaag
    14341 gccgccgttg atgcggggag cgctgcggca agcgccgttg gcgaagtgaa atcctgccat
    14401 gtcatcccgc gcccacacag tgatgttgag gctattttac ctaaatcggc ataaccccat
    14461 ttgctaagga gcacagcgtg aagcaatcac tgggattact tgaagccagt ggtctggctc
    14521 tcgccatcag ttgcgcagac gctatggcaa aagccgcgtc catcacgctg gttggccttg
    14581 agaagaccaa tggctcaggc tggacgctca tcaaaatcac cggtgacgtc gcctcggtta
    14641 atgcggcgat cgcgacggga gccagtttcg ccggacagcg aaatggcctt gtcgcacaca
    14701 aagtgattgc ccgcccggga gacggcattc tgccccacac aactgaagca ttgaccgtga
    14761 cggttgaaac aagcaaaaca ccagcaacaa ccgtcacctc agagattatc agttgtaatt
    14821 tgtgtcttga cccggcatgt cagcggcaaa aaggtgagcc ccgctcactt tgcctgtacg
    14881 ccaataagcg aggggagtag cagtggataa acaaaccttg caggaaacag tgagccagat
    14941 tctggatgag atgcgcgagc gccctatccc actgggcgtt tccagtcgcc atatacatct
    15001 ttcatcagcg gattatttgc gtctttttcc tgaacaaccg attagcgaga aaaagccgtt
    15061 aatgcaacct ggccaatacg ctgctgaaca ggttgttacg ctggagggac caaaaggcaa
    15121 attgcacaac gttcgcttgt tggggccgct acgcaatgca agccaggtgg aaatttcccg
    15181 gacggacgcc agaacgttag ggatcaccgc cccgctacgc atgtcaggcg atctcagcgg
    15241 cactccgggg attcgtttga taagcccatt tgcggaactg gagatcacat ctggcgtcat
    15301 tgtcgcgcaa cggcatattc atatgtctcc gctggatgcc ctgatttatg gcgttacgca
    15361 tggcgataaa gtggcggttg ccattgaagg tgatgagcga cgactgatat tcgaaaacgt
    15421 cgctgttcgc gtctctccgg atatgcgtct tgaaatgcat attgataccg atgaggccaa
    15481 cgccgcaggc gctgatagcc ctgacgtttt tgccacactg gtgtcagtgc catgaataac
    15541 atgctgatgc aacgtattgt tgacgaagtg gtttttcgcc ttaaacaacg tgcgggaaaa
    15601 acactcgtgc tgactgtttt tcaattgcga gacgccagcg ttcaagagag cgttcatcag
    15661 tatgcgtcat tacaaatccg atatgtcgat ctgccgctgc tgcgacaact tgcggagaac
    15721 gaaacgtcag atcgagcatc tatccagata cacgaagcgt tagcctgggg aatgcacatt
    15781 caattgtcgt tgcagcgtca tttcctgaac gccatcgaat tgaagactct cgcccgtcta
    15841 ccgctaagct ggtgcgacga acaagggcag ccgatctacc ttcatcataa tcgactttta
    15901 agttatgccg atatcgcaca gttaagctcg ggaatactgg tgttacagcg gaaatgttgt
    15961 gtgaccgcac tggctcgtga agcggcaatt acccgaaata ttcagttaat caggcaggag
    16021 taacctatgc atctggctcg agtcactggc gcggtagtat ccacgcaaaa atcaccttct
    16081 ctgatcggga aaaaactgct gctggttcgc cgggtcagtg ctgacggcga actgcctctc
    16141 acgccattaa ccggcgacga agttgccgtt gattcagtcg gcgcaggcgt tggtgagttg
    16201 gtactcctca gcagtggttc cagcgccaga catgtgtttt caggccctaa cgaggccatc
    16261 gacctggcag tggtcggcat tgtcgatacg ctttcgcgct aaggagctaa aagatggcaa
    16321 tttatacccg aacaggtgat tcaggaagta cgtctttatt taccggacag cgcgtcagca
    16381 aaactcatct acgtgtcgaa acctatggca cgctggatga actgaacgcg acactcagtc
    16441 tgtgttattg cgccaccgcc atcgaaagcc atcgcatctt actggaagcc attcaacaac
    16501 agatattttg gtttagcgcc gaactcgcca gtgaaagcga acagccgtca gcgcaacagc
    16561 gttacatcgg tacggaagaa attgccgcgt tggaaaacgc gatcgatagt gcaatgaacg
    16621 ccgtcccccc agttcacagc ttcattctgc ctggtcgatg tgaagccgca agccgcatgc
    16681 atttcgcccg cacggtggct cgacgggctg aaagacggct ggttgaactg acaacggaaa
    16741 ccaccgtccg gaatgttttg ctgcactata tcaaccgact ttccgattgc ctgtatgccc
    16801 tcgcccgcgt ggaagacaac gttgctcatc aaaacctgat gattcaggaa atcacaaaac
    16861 gttatcacga agccaaccac acaccagcat tgaaggaacg cacaatgccg ctcacttttc
    16921 aggatcttca ccagctcatt cgtagtgcgg caatgcgcgc ggatgaactg catattcccg
    16981 ttgtcatcag cattgttgat gccaacggaa cagaaagtgt tacctggcgg atgcctgatg
    17041 ccctgttggt cagcagtgaa ctggcaccga aaaaagcctg gaccgccgtg gcgatgaaaa
    17101 cggcaaccca caagcttgct gataccgtgc aacccgacgc gccgctttac gggctcgaaa
    17161 gccatatgca aggaaaagtc gtcacttttg gtggcggctt ccccctctgg cgtgacggaa
    17221 aattgcttgg cgggcttggc atcagcggcg gtagcgttga acaagacatg gatattgccc
    17281 aaagcgcaat ggcggcaatt aacgtgggag taaaccaatg aataactctg aattagaaac
    17341 actgatccgc aatattctgc gtgagcaact tgtccccgct acaccacgaa cgcaaagaaa
    17401 cgctattttc cagactgtcg atgaagcggt ctgcgcagca cactaggcct ttttacgctt
    17461 tcaacagtgc ccgcttaaaa cccgtagcgc cattatcagc gccattcgcg aagaattaat
    17521 gccccgcctt accgaactgg cagaagaaag cgccaaagaa acggggatgg gaaataaaga
    17581 agataagtac ctaaaaaaca aagcggcact cgataacacg ccaggaattg aagatttaac
    17641 caccacagca ctaaccggtg atggtggcat ggttttgttt gagtactctc cgtttggggt
    17701 gattggctcg gtcacgccaa gcaccaaccc cacggaaacc atcattaaca acagcatcag
    17761 catgctggca gcaggtaata gcgtctactt cagtccacac ccgggtgcca aaaacgtttc
    17821 gttaaaactg attaccacaa tcgaagacat cgcgtttcgc tgtagtggca ttcataactt
    17881 gatcgttacc gtatctgaac cgacctttgc tgccaccgaa cagatgatgg cgcaccctaa
    17941 aatcagcgtt ctggcgatta ccggcggtcc ggggatcgtc gccatgggaa tgaaaagtgg
    18001 caagaaagtg attggtgccg gagcgggtaa ccctccgtgc atcgtcgatg aaacggcgga
    18061 tattgtgaaa gccgccgagg acatcatcaa cggcgcttca tttgattaca acctgccgtg
    18121 tattgctgaa aaaagcctga ttgtcgtggc atcagtggca gattatttaa ttcagcagat
    18181 gcaaagtttt ggcgcacttc ttcttaacta tgagcaaact gagaaattgc gtgcaatttg
    18241 cctgccagac ggtagcgcca acaaaaaact ggttgggaaa agcccatcag ccttgctgga
    18301 agcggcaggc ctccctttgc ccgcaaaagc gccgcgccta ctgattgctg ttgtcgacgc
    18361 cgatgatagt tgggttactt gtgagcaact aatgccaatg ctgcccattg tgaaggtcaa
    18421 ggattttgac agcgctctaa ccctggcgct gaaagtggaa gacggcttgc accacacggc
    18481 aattatgcac tctcagaacg tctcacgact caaccttgcc gcgcgcgtta tgcaaacctc
    18541 aatatttgtc aaaaacggcc cctcctgggc gggtattggc gtcggcggcg aaggctttac
    18601 gacctttacc attgcaacac cgactggaga gggtacaacg tcagcacgga cttttgcccg
    18661 ctcacgacgc tgcgtgttaa ccagcggctt ttcaattcgc tagagaaacg gaaatgaata
    18721 ctttttcact gcagacgcgt ttatacagcg gaccaggaag tctcaaggta cttagccgtt
    18781 tctctcatag acacatctgg attatttgcg atgcctttct ggcacgctca ccacttattg
    18841 acacgttgca tcaggcgctg cccgacagta atcgtctcag tatttttagc gaaataacgc
    18901 ccgccccaac tatccagacc gtggtgaaag gcattgcgca aatgcagact ttacgcccgg
    18961 atgtagttat cggttttggc ggcggttctg ccctggacgc cgcaaaagca attgtctggt
    19021 tcggtcgcca gtgtggcatt gaaattgaaa cctgcgtagc cattcctacc accagcggta
    19081 tcggttccga agtgaccagt gcttgtgtca tcagcgatcc tgagaaaggg attaaatatc
    19141 cccttttcga taatgcactt tatccggata tcgcgatcct tgatccatct ttggtcgtca
    19201 gcgtgccacc ggcgataacg gccaataccg ggatggatgt tttaacccat gcgcttgaag
    19261 cttatgtttc tcctcgcgcc agcgacttca cggatgcact ggtcgaaaaa gccgtgcaaa
    19321 ttgtttttca atacctgccc actgcagtga aaaaaggcga ttgtcttgcc acccgtggca
    19381 aaatgcataa tgcatcgacg cttgcaggaa tggcctttag ccaggccgga ctcggtttaa
    19441 accatgcaat tgctcatcaa ctcggcggac agtttcatct gccgcatgga ctggcgaatg
    19501 cgctcctgct cacggcagtt atccgcttta atgctggcga tccgcgcaca gccaaacgct
    19561 atgcccggct ggcgaaaacg tgccatttat gcccggacaa cgccaatgac accgccagcc
    19621 tgaatgcact tattcaacat attgagcagc ttaatacaac ctgtacgctg ccgactcttg
    19681 ccaatgcgct gaaagaaaaa aaagcagaat ggtcaatacg cataccagat atggttcagg
    19741 cggcgctggc ggatgcaacg ttgcgtacca acccgcgtgc agctgatgcc tccgcgattg
    19801 cagaactgct caaggggttg ttatgaacgt cggattaatc tcctgcgacg accagaccat
    19861 ccgcgagcga gtacaagcag caggtgtcgt gggtgctggc ggcgcaggtt ttccaaccca
    19921 tattaagcta caagcgcgtg ctgacacttt cctggtgaat gcggcggaat gcgaacccat
    19981 gttgaaagta gatcaacagt taatggcgca gcaagccagc cgcctgcttc gcggtgttca
    20041 ctatgcgatg aaagccaccg gggccagtag cggaatcatc gcgctgaaag aaaaatatca
    20101 acgggcaata aacgcactca caccactgtt accaccggat atccgcctgc atattttgcc
    20161 cgacgtttac cccgcaggag acgaagtgct gaccatctgg atggcaactg gtcgccgcgt
    20221 tccacccgct gcattacccg ttagcgtcgg tgtggtggtc aataatgttc aaaccgtact
    20281 taatattgcc agagccgtcg agcaacagta tccagtgacg cgccgcacac tcaccgttaa
    20341 tggtgcagtg gcgaagccaa taacggtaac cgtccccatc ggcatgtctt tacgtgaagt
    20401 cctggcgctg gcgggcggcg caacagtgga tgctcctggt tttatcaatg gcgggccgat
    20461 gatgggaagt ttgatcacat cgctcgacac accagtcagc aaaaccactg gcggcttgct
    20521 ggttttaccc aactctcatt cactgatcca acgtcgaatg cagaatgacc gcagcgtact
    20581 tgccgtcgcg aaaactgttt gtgagcaatg ccgcttgtgt accgatctct gcccacgtca
    20641 tttaatcggt cacgaactgg caccccattt attagtacgt gcgcggttaa ctaccaacag
    20701 ctcgccacgc cgcaactgtt actgacagcg ctcacctgtt cagagtgcaa cgtctgcgcc
    20761 agtgtcgcct gtccggtagg aatttcacca atgcgcataa accgcctgct taagcaggaa
    20821 ttacgcgcac aaaatctccg ctacgaaggt gccttaaatc ctgcagatcc catggcaaat
    20881 tatcgactga ttcccgtaaa acggctggta accagacttg gcttaacccc gtggtatcag
    20941 gatgcgccat tgtctgaaca ggtgcctcag cccgaaaaag tgacgttatt gttacgccag
    21001 cacatcggtg ccagcgcaat tgcgtgtgta caaaaaggcg atcgcgtcgt tcatgggcaa
    21061 tgtgtaggtc aaattccaca cggcacatta ggtgccccca ttcatgccag tattgatggc
    21121 atggtcagcg atgtgacaga aaacgcgatt acgcttgtga gaggttaacc atgtcgaaag
    21181 cggtaggtat tttagaactt agcagtatcg cgaaagggat ggaaaccggc gacgccatgc
    21241 tgaaaagcgc caacgttgag ttactggtca gcaaaacgct atgccccgga aaatttctgc
    21301 tgatgctcgg tggcgatgtg ggcgcggtgc aacaggccat caacagcgga gcgtcccttg
    21361 ctggtgaaat gctggtcgac agtcttgtcc tgccgaatat tcatcccagc ctcctttctg
    21421 caatcagcgg acttaaccat gttgaacagc accgggctgt cggcgtagtc gaaacctgga
    21481 gtgtcgccgc ctgcattgac gctgccgatt gtgctgtcaa agctgcaaat gtcattcttg
    21541 ttcgcgtaca tatggctttt ggcattggcg gtaaatgcta catggtggtt gctggcgata
    21601 tttcagatgt taataacgcg gtctctgccg ccagcgtgcg cgcgggggaa aaaggtttgt
    21661 tggtctatcg gtcggtgatt gcccgtccac atgaagccat gtggcgtcag atggtggagg
    21721 gataatgaca actcagcaca caactgaacg gatgattcag gaatatgtcc caggtaagca
    21781 ggtcacgctg gcacatgtca tcgccaatcc agggaaggat ctgtttaaga aattgggttt
    21841 accagacacc atttccgcta ttggtattct gacaattacc cccagcgaag catctatcat
    21901 cgcatgcgat atcgccacaa aatcaggcgc tgtagagatc ggttttcttg atcgttttac
    21961 cggtgccgta gtgctgactg gtgatgtttc tgcgatcgaa tatgcactta gccaggttac
    22021 ccgtacgctg ggtgaaatga tgcgctttac cgcctgcccc attacgcgga cctgacgtta
    22081 tgcaacgtat gatgctcatc ggccccagcc agtgcggaaa aacgtcactc acgcaatgtc
    22141 tgcgtggcga aaatattcat tatcagaaaa cgcaggcaat cgtctggtcc ccgaccacca
    22201 ttgatacacc aggcgaatac ctcgaaaacc gcagcctcta cagtgcactg ttggtcagtg
    22261 cctgcgaagc ggacattatc gcgctggtgc ttaatgcgga cgcaccgtgg tcaccttttt
    22321 cgccaggctt tacctcgtca atgaatcgcc cggtcattgg agtagtaacc aaagcagatt
    22381 tagccaacat ggaacaaata tctttggtga aatgttggtt acgggaagct ggagcgcaca
    22441 acgtgttagt aaccagtgcg gttaataacc atggagttgc agaactttta gccttgctgc
    22501 atacagaaga aggctgtcgc taatttcgac aggttatcag atgggaatga tgacggtgat
    22561 acacagaaaa gaatcaatga gcaatgaggg ttgccgggca accctcattg aataaaacag
    22621 gaattacttc gcttcgccgt ttttcatttc gcccatagct ttcagctttt tggagatatc
    22681 gcggcgttct ttggagagct cggcattttt gatgatgtaa tcgtcaacgc gatcttcata
    22741 gtcacctttc atgctggcga tgatgccctg gattgcttca acgctcatcc caggcttgat
    22801 gtaatcgctc aggttgtcca gcagcaggac gcgcttctgg ttgtcacgga tctttttctc
    22861 aacgtcctga atttcacgtt gcagtttgtt cttacgacgg aacagacgaa caaattccag
    22921 tacgtcctga aatgaaggct tggtagtttc catttttata cccctgataa tgtgagagtc
    22981 ggattcgttt aagcaaccgc tattcgttag ggccaacatt actcatagct gccgcagatg
    23041 acaatgcttt tatccctttt cactatcata ccctttatcc ttgctgaatc gaagcagcag
    23101 caagatgatt ctgaagttca ggaattattt gcgcaaggcc cggcaaatca gattcgatag
    23161 caactcaagc tgatgcgccg tttccatgtt cagccacaca taaccataga ttggtgtttc
    23221 cactaccttc aggtcatggt ggttattgag caactgacgc agctcttcca cagcatcgtt
    23281 caatttttcc gtattggcaa aaaccggctg cggattacct tcgtacagcg catgaacaaa
    23341 gctcagcagt atttgctgca tcatgtgctg ggtatcacga agtttttgcg cgttcaataa
    23401 cacgaaatgg ctggggcgcg tggcccagta cgcattgatt tgcaactcca gcatacaaac
    23461 cagattgcgg ttaatggtct ggataccttc atatatcgat tttggaatac gggtttcttt
    23521 gctggcgggc gcaatcagcc cacgcatttt cacggcatcg gtcaggagtt tttgtagatg
    23581 gctttccaga cgtgggcgtt cgagtaagtt cggtgagaat gcagattgat agacccgatt
    23641 atactcggtc agacttttcg ccagttgaat gcgccagtgg atgaacgccc gttgtggcca
    23701 gataccggta aacaacattg ccagtaaaga gccgaggatc acatcgccgc ttcgccataa
    23761 cgccgtatca atttcacctg tcggggaacc aacaacaatt gccagcgtca ccccaatcaa
    23821 taatccttga tacggtttct tgcccagcgc cagccaaccg caaagaaaca tcgccgccgc
    23881 gcaccagact aacatcagcg gtaacgaaat taactccagt tgcagagcga taagacctaa
    23941 aatcgaaccc aacaccgtac cgccaatacg ttcgaaggca cgagggacaa cgttacccca
    24001 gaacgagatt ggccccataa tcaccaccat ggtgaccagc ggccaggtgc tttccgggat
    24061 agtaaacagg cggataatga gaaaagtgag caggaatgcc agcgcgaccc gagtaccatg
    24121 cactatgcgg taatggcggt atacccggat ttcaaacggg cttaatgact tatcggcacg
    24181 cacgcagaaa ctccgtaatg aagattaaaa gcgctcataa ctcatctgtc ggccagaagg
    24241 cgcgcctctg aattatccga cggacaaacc gggaacagtg taatttcccg gcaggagcga
    24301 aaaaattgaa aggcgcatct tactcttttc gctttcaaaa aaagagtggt catcgcgtta
    24361 acacaccgcc ctgagatgaa ttagtgatgt tttggctgca ccgcaacata catttcgata
    24421 tcccagtacc catcttccgc gccattgttc aaataaacct cgaagcaggg ctttggtaac
    24481 atttcataag cactgtcctg caagaggcta ttaaagaact gataccaggg tttagcaaaa
    24541 tcatcaccga ctacacgagc caccgccacc gcatactgac cacctgaaat ttctgtcaga
    24601 atgacgccct cactgttttc gggaagcgta aagttattcg gtaccgtcac aacggtgtcg
    24661 cagcgtaatt tttcggcggg tgtttcatct ggattgtcgt aatagacagc aacccactcc
    24721 ttcggcacaa tatttttgtt atctacccac atcatcaact gctcaaagcc tttctttacc
    24781 gtctgttccc acgggccaac gagatggaaa cctgcaacgg tacgtttctc ttcctgcgta
    24841 atctcgtagt tcatgacgcc tccattaaca ctgtttttat atacagcata gttgcaatat
    24901 taaaaccaca aggaatgagt gttgattatg cgagcagact cgcactcctg ccagtctgct
    24961 gcaaaagaaa ggtcaggcct tatggtggaa ataatcactc aggcgggaaa acatgccgcc
    25021 ttccccgaca gattccaggg taaccagcgg ccagtgcgcc acctgtttat cacggtcgta
    25081 aagttcaatt tcccctaccc gctgatgggc gctaattggc gcggtgagct ctttaccatc
    25141 aagggtatat ttggctttga tatgtggaat ttcggctttc ggtagcacca tccagaactc
    25201 ttgttccgtt cctaggtcga tattttcttt atcgccatac cagatgcgtt ccgttccgac
    25261 ctttttccca cggtgcaaaa tttgcaccgt agtaaagttt tgttgccccc aacgcagtaa
    25321 ttttcttgcc tcttcctcac gaccttttgc actgtcagcc cccattacca ctgcaatgag
    25381 acgacgctgc ccatctacag ccgaagcaat gagattaaac ccggcaccag aagtatgacc
    25441 cgttttcagg ccgtcaacat tcatggtttt atcccacaac aacccgttac ggttttgctg
    25501 ggtgataccg ttccaggtga gacttttctc actgtacata tgataaaact cgggctcgcc
    25561 gtggatgata gcgcgagaaa acacagctaa atcataagcc gagctatgct ggccaggtgc
    25621 atccagacca tgcactgttt caaaatgcgt atccttgaga tgcagcttct cggcatagtt
    25681 gttcatcatt tcaacaaact gccgttgccc accggcaata tagtcagcca gagcaacaca
    25741 agcgtcattt ccggaatcca caattaaacc acggcttaaa tcacgtaccg atacgcgatc
    25801 gccctctttc aaaaacatca gtgaagaacc gacaaacacc ggattatctt tcgcccacgc
    25861 atcgcgcccc acggtgacaa tatcgtctgg cgtaatgcga tgactatcga tagcgcgatc
    25921 cacgacataa cccgtcatca gctttgtcag gctggcggga ttgcgctgtt gatgctcatt
    25981 acccgcggtg aggatctgac cggtggtgta atccatcaat acccaggacc cggcatgaat
    26041 ctctggaggc tgaggtgaaa aaggaatgtt ttccgccgca aaaccagacg ataagttaaa
    26101 aacgaacaaa gaagcagcaa taataagacg gcgtttcaac agcaaaccct caggagtttc
    26161 aaatagctgt tctttttacg gaaatactta tgaactggct ggaataaagt gcaagaaaat
    26221 gtgactaccc tctcattttt atctgacatg atctgttgcc actcgctgcc aaattgtggc
    26281 gctaaagctg attagcacgg tgatatttga tactctggca gacagcagaa ataacggatt
    26341 taacctaatg atgaatgacg gtaagcaaca atctaccttt ttgtttcacg attacgaaac
    26401 ctttggcacg caccccgcgt tagatcgccc tgcacagttc gcagccattc gcaccgataa
    26461 cgaattcaat gtcatcggcg aacccgaagt cttttactgc aagcccgctg atgactattt
    26521 accccagcca ggagccgtat taattaccgg tattaccccg caggaagcac gggcgaaagg
    26581 agaaaacgaa gccgcgtttg ccgcccgtat tcactcgctt tttaccgtac cgaagacctg
    26641 tattctgggc tacaacaatg tgcgtttcga cgacgaagtc acacgcaacg ttttttatcg
    26701 taatttctac gatccttacg cctggagctg gcagcatgat aactcgcgct gggatttgct
    26761 ggatgttatg cgtgcctgtt atgccctgcg cccggaagga ataaactggc ctgaaaatga
    26821 tgacggtcta ccgagctttc gccttgagca tttaaccaaa gcgaatggaa ttgaacatag
    26881 caacgcccac gatgcgatgg ctgatgtgta cgccactatt gcgatggcga aactggtaaa
    26941 aacgcgtcag ccacgtctgt ttgattatct ctttacccat cgtaataaac acaaactgat
    27001 ggcgttgatt gatgttccgc agatgaaacc cctggttcac gtttccggaa tgtttggggc
    27061 atggcgcggc aataccagct gggtggcacc gctggcgtgg catccagaaa atcgcaatgc
    27121 cgtaattatg gtggatttgg caggagacat ttcgccatta ctggaactgg atagcgacac
    27181 attgcgcgag cgtttatata ctgcaaaagc cgatcttggc gataacgccg ccgttccggt
    27241 taagctggtg catatcaata aatgtccggt gctggcccag gcgaatacgc tacgcccgga
    27301 agatgccgac cgactgggaa ttaatcgtca gcattgcctc gataacctga aaattctgcg
    27361 tgaaaatccg caagtgcgcg aaaaagtggt ggcgatattc gcggaagccg aaccgtttac
    27421 gccttcagat aacgtggatg cacagcttta taacggcttt ttcagtgacg cagatcgtgc
    27481 agcaatgaaa attgtgctgg aaaccgagcc gcgtaattta ccggcactgg atatcacttt
    27541 tgtcgataaa cggattgaaa agctgttgtt caattatcgg gcacgcaact tcccggggac
    27601 gctggattat gccgagcagc aacgctggct ggagcaccgc cgccaggtct tcacgcccga
    27661 gtttttacaa ggttatgcag atgaattgca gatgctggca caacaatatg ccgataacaa
    27721 agagaaagtg gcgctgttaa aagcgctttg gcagtacgcg gaagagatcg tctaatttga
    27781 agcctccgcc gctggtacgg cggaggtcaa tgttgagtca ggctttttga acggtgatac
    27841 tccacgctgc atcgccaatc tgctgataat cggtgatggc atgcccttct tctgccgccc
    27901 actgtgggat tgcttcagtt gcctgggtgc aatcaaactc aattaccaac tcatcgccgc
    27961 tggccatttc tgccagtgct gcttttgctt ctatgagtgg aaacggacag acctgcgtca
    28021 ccacatccag ctttttaatc gccatcttta caccttactt aatttgccgc agcagttgcc
    28081 agtcgcgcct tacgctgcgg tcgaacatac acaagccagg acgcagtcca gactccgaga
    28141 atcataaata caaggccaat ccagccctgc caggtcatca ttgcagtcat aaccagtcca
    28201 ttaccgataa agcaaccacc tgcaatactg gcaccgaagc ccatcagtac accacctaac
    28261 ccgctacgta atgttgtttg tgcatcagct gcgcgaacgc ggaactcacg gctcgctttg
    28321 gctgcaataa atgaccccac gaagatccct aacaccagga aaacgcccca gttaatgtat
    28381 ttcacgtcac ccgcgaccag aaattgcaga atattggccg ttggggaagt gattcctaac
    28441 ccaaacatgc gtccggttgc ttcactcagg ggccaggcta aaagcgcaat caaaccgatg
    28501 agtacagcag tgacaaaggg atgccagcgt ttttcaaaca gaatatgagc gatcccagtt
    28561 cggcgcggcg gtaaggtcgc gactttgagt tttggcttct tcaactcttt catcaccacc
    28621 cagagcgtta tcaccagcaa aacggcaacc aacggccaaa cagacaaatt aaaagtctca
    28681 gcaatagagt tatgttcagt actgtagtgc tgcaaggttt gatttaaacc actggcatgt
    28741 ggagaacgca tcaccgcact catcaccata taagtgaaaa gcgcgatcca actgccgatc
    28801 aatccctcac ccgcgcgata ccaggtcccg gtggcacatc cacccgccag aacaattccc
    28861 agcccgaaga tatacccacc tataacagta ccaagccacg ggaacgcctc ggcttcgtaa
    28921 gtcagtaggc ccgcctgaat taacgcaaaa acccccacgc tttgtaccga aattgcaatc
    28981 agcaaggcgt aaaacatgcg attatttttc gcgatataca tatcgcgaaa accacctgtc
    29041 aggcaaaaac gcccacgctg catgacaaat cccagcagag caccacaaat tagcccgctt
    29101 aatatcattg aaaacataaa ttaattaacc agatgaatgt taatgaggaa attattcatg
    29161 actggtggaa tgcagaccaa taaccaaatt ctctaaatta gaacaaatgg ttattaatga
    29221 ggatttcgat aaaaaaaaat aaaaaaaccg gagccaaata ttcattcgac tccggttgat
    29281 tattagaaaa gatgattatg caacgtcttc gtactgcgga actgggttac ggaagctctt
    29341 ggtgacgcaa gccaggtaaa tcagaccgat agcagcccaa atcagaccca gaaccattga
    29401 gctttcttcc aggttaaccc acagcgcacc aacggtcagc gcaccacaca tcggcaggaa
    29461 cagatactgg aagtgatctt tcagcgtctt attacgcttc tcacggatcc agaactgcga
    29521 gatgaccgac aggttaacga aggtgaacgc caccaacgca ccaaagttaa tcagcgctgt
    29581 agccattacc aggtcgaagt tgattgccag cagggcaatc gcgccaacca ggatgatgtt
    29641 catcgccgga gtacgccatt tcgggtgtac ataaccgaag aagcttttcg ggaatacgcc
    29701 gtcacgaccc attacgtaca tcagacgcgc tacgcctgca tgcgccgcca taccggacgc
    29761 cagtacggta atggtggaga agatcagcgc gccaacctgg aacgctttac ccgcaacgta
    29821 cagcatgatt tcaggctgtg acgcatccgg atctttaaag cgagagatat ccgggaagta
    29881 cagctgcagg aagtaagttg caaagatgaa gatcatgccg ccaatcagcg cggtcaggaa
    29941 aatcgcacgc gggatcacgc gctctgcatc tttggtttct tccgacaggt tgctgatgcc
    30001 gtcaaagccg gtaaaggaaa agcacaggat tgtcgccccg gtaatcatcg gaataacatg
    30061 cgcatcgcca gaccagaacg gacgagtgct cgccagcgta ccagcgcctt caccttcaaa
    30121 tacgccataa acaaccatgc ccagaatcac cgcgatcagc actacctgca acacgacgat
    30181 tacagtgttg aagttcgcta cggatttcag actacgcagg ttaaaggcgg tcataaaggc
    30241 caccagcgcc accacaaaca tccacgatgg gatggaaggc accagagctt caaaatagat
    30301 tttcgccagc agaatgttga tcatcggcgc gaacagatag tcgagcagag aagaccaacc
    30361 caccataaag ccgacagtcg ggctaatgga tttctgggcg taagtatatg cagagccagc
    30421 agaaggatag cggcgaacca gcttcccgta gctcagagcc gtaaacagga tcgcaatcaa
    30481 tgcgaacgca taggctgtcg gcacatgacc atccgtgagg cctgaaacga taccaaaagt
    30541 atcaaacagc gtcatcggct gcatataggc aagacccatc attacaaccg gaactaacgt
    30601 aagcgtttta cgcaattcca cgcgagaggt gtttggagta acgttatgcg acatggtcat
    30661 tctcctttac ggtgataacc gtcgcgtaag caaaaaattg ccccattttt ttggattcct
    30721 cagcgacaac aactgtcgat ttttagtaaa tatctatccg gtacgaagcc cggcctcttg
    30781 gtatgaatga ttggtttgaa gcaaaaaata accgacgctg atgaaacgtc ggtttttagt
    30841 cattttttga cagcggcgca ttgtgcctaa atggggggga aatgacaaga gaatgagaag
    30901 cttgtcataa taatttttct ttaaatggct gattttccgt catcagattt tgcataaaca
    30961 ccggcgatcg cactatttgc taaaatttca tcccgccacc agccgctggc aagccctgcg
    31021 gtctgttcgt tccagccaat ccagacgggt tcaaagagag tttgggcgac cactttcttc
    31081 tcgattaacg cgccactatc gagaaaacgt tgcaccagat aacggggtaa atagccgcag
    31141 ccgaggccgc taatttgcag ctccagcttg gttttaaaat cgaaaacggt aatggcttct
    31201 tgttcgtcga gaagttgtga ggccgtagaa gcgcctgcct gcgcagtgtc ccccaccaca
    31261 atcgcccgat atcgcttaat gatacgacgg tttaacggct cttcttcctg ggctaacgga
    31321 tgatgaggcg cgaccgcaaa gacttgttcc agatcgccca accgcgagaa accaaactca
    31381 ctggaagatg gtggctcatg catcgcgcca acaataatat ccgctcgccc ctgagtcagg
    31441 gcatcccagg aaccaccgag tacgccattg ataaacttca ggcgcgtcac gctatgatgt
    31501 tgataaaaag cttcaatcag tggcgcaagc agagaaaaag gaaacgtgtc gtccacgcca
    31561 atcaccagct cgttttccca gccttcatgc agtttgatcg cctgcttttc cagttctcgc
    31621 acggtatgca gaacttcccg ccccttctct aataacattt ttccggtacg ggtgaattta
    31681 gcgcggtgac cgctacgatc aagtaattga atattaaggt cgctttccag cttgtgaacg
    31741 gtgtaactca gagcggacgg cgtcttgtag agtttagccg acgccgccgc aaaactgcct
    31801 tctttttcta aggcatcgag gatcatcaac acatccagca gttgtttcat acacgccccc
    31861 ttgcagtgca tgcggcgtcc tgctggcgtg atcgtttact ccagcggcat taccagcgga
    31921 tcgggatact ggtattcaaa tcccagttca ttacaaatcc gactgccatc aataatctta
    31981 cctttgccgc tgtccagact atttctgaac tgcggaggtt ccagccccag taaacgggcc
    32041 atctgcggat agaaaacatt acgcgcaggg tgagctggcg cacatatatt atagatgtgt
    32101 ccgcctttag gtgcctgtaa caacagagtg atagcgccaa taacatcttc taaatggact
    32161 aaattaacac catgttcacc atcaggcgcg gtttttccgg caaagaagcg tccgggatga
    32221 cgtcccggtc cgaccagccc cgcaagacgc agaatatcga ccgaagtacc gggtaaattg
    32281 tgcagccagt cttcgagttc ttctaatact cgtccactgt tggtcaccgg attacgcggg
    32341 gtggtttctt tcaccgtgcc ttgcgcgtcg ccatagacag acgttgagct ggtaaaaata
    32401 atgcgaggaa tacgatgggc cagcgcgcta tccactaact cttgtaccgc ttgtaaatag
    32461 aactcatcgc cggggccgct acgacgtgcc ggaagcgtaa tgaccagcgc atcggcatcc
    32521 atcagggcat ccagatcgtc agaatcgcaa actaactcag gctccatgcg aagcagatag
    32581 ctatcaatgc cactcattcg ggccgcttcg acaccatctt gtgtggtttt actcccggtg
    32641 acttgccagc ctcgcgctga aagtgacatc gccagcggca tgcctaacca ccctaacccg
    32701 acaattgcga cctttttcat ccgttatctc ctgatacacc ttagatctat aaggctacgc
    32761 tagcgtatca aaactgacaa ttcattctat gaatgaatct gttcaataat gataacgaca
    32821 tgctgcaatg agcagtgaat cgtcggtaac cgcgtatacc agacggtgct cctctgtaat
    32881 gcgtcgggac cagaaacctg acaaattatg tttcaggggt tctggcttcc ccttaccttc
    32941 aaatggcgtt ctgcgggtat ctttgataag ttcattgatc tttttaacaa ttcgcttatc
    33001 tgtttcctgc cagtacagat aatcatccca tgattcctca gaccagatta gtttcactca
    33061 ataatgtcct tttctgttcc tttgcctgat ttcaggctat cgattgagtc catcaatctc
    33121 cgggcgttag cgggggagcg cagtagataa gccgtctctt ccagcgagtt gtattcttcg
    33181 agtgacatca gaacacaagc ctctccattc tgacgagtaa taaggatcgg ggcatgatct
    33241 tcaacggctt tcatcattgt tgccgacaaa ttctgacgcg cttcgctgta gctaattgta
    33301 cgcatgtcaa tctcctcttt tgtacagttt attgtacaat gatgggcgtt aattaactat
    33361 ttattaatta gtttgtagat caaggtattg tcagtgagac gaaaatccag gcttcgctat
    33421 ttttggtgcc gtcagcttag aggacagtcc tcttagcccc ctcctttccc cgctcattca
    33481 ttaaacaaat ccattgccat aaaatatata aaaaagccct tgctttctaa cgtgaaagtg
    33541 gtttaggtta aaagacatca gttgaataaa cattcacaga gacttttatg acacgcgttc
    33601 aatttaaaca ccaccatcat caccatcatc ctgactagtc tttcaggcga tgtgtgctgg
    33661 aagacattca gatcttccag tggtgcatga acgcatgaga aagcccccgg aagatcacct
    33721 tccgggggct tttttattgc gcggttgata acggttcaga caggtttaaa gaggaataac
    33781 aaaatgacag acaacactcg tttacgcata gctatgcaga aatccggccg tttaagtgat
    33841 gactcacgcg aattactggc gcgctgtggc attaaaatta atcttcacac ccagcgcctg
    33901 atcgcgatgg cagaaaacat gccgattgat attctgcgcg tgcgtgacga cgacattccc
    33961 ggtctggtaa tggatggcgt ggtagacctt gggattatcg gcgaaaacgt gctggaagaa
    34021 gagctgctta accgccgcgc ccagggtgaa gatccgcgct actttacctt gcgtcgtctg
    34081 gatttcggcg gctgccgcct ttcgctggca acgccggttg atgaagcctg ggacggcccg
    34141 ctctccttaa acggtaaacg tatcgccacc tcttatcctc acctgctcaa gcgttatctc
    34201 gaccagaaag gcatctcttt taaatcctgc ttactgaacg gttctgttga agtcgccccg
    34261 cgtgccggac tggcggatgc gatttgcgat ctggtttcca ccggtgccac gctggaagca
    34321 aacggcctgc gcgaagtcga agttatctac cgctcgaaag cctgccttat ccagcgcgat
    34381 ggtgaaatgg aagaatccaa acagcagctg atcgacaaac tgctgacccg tattcagggt
    34441 gtgatccagg cgcgcgaatc aaaatacatc atgatgcacg caccgaccga acgtctggat
    34501 gaagtcatcg ccctgctgcc aggtgccgaa cgcccaacca ttctgccgct ggcgggtgac
    34561 caacaacgcg tagcgatgca catggtcagt agcgaaaccc tgttctggga aacgatggaa
    34621 aaactgaaag cgctgggtgc cagttcaatt ctggtcctgc cgattgagaa gatgatggag
    34681 tgatcgccat gagctttaac acaatcattg actggaatag ctgtactgcg gagcaacaac
    34741 gccagctgtt aatgcgcccg gcgatctccg cttctgaaag cattacccgc actgttaacg
    34801 atattctcga taacgtgaaa gcacgcggtg atgacgccct gcgggaatac agcgcgaagt
    34861 ttgataaaac cacggttaac gcgctgaagg tgtctgctga ggagattgcc gccgctagcg
    34921 aacgcctgag cgacgagcta aaacaggcga tggcggtggc agtaaaaaat attgaaacct
    34981 tccacactgc gcaaaaactg ccgccggtag atgtagaaac gcagccaggc gtgcgttgcc
    35041 agcaggtcac gcgcccggtc gcttcagttg ggttgtatat tcctggcggc tccgccccgc
    35101 tcttctcaac ggtattaatg ctggcgactc cggcgcgtat tgcgggctgt aaaaaagtgg
    35161 tgctgtgctc accgccgccg attgccgatg agatccttta tgcggcgcag ctgtgcggtg
    35221 tgaaggacgt gtttaacgtc ggcggcgcac aggccattgc cgcgctggcg tttggtacgg
    35281 aatctgtgcc gaaagtggac aaaatcttcg ggccgggtaa cgcctttgtc accgaagcga
    35341 aacgtcaggt gagccagcgt ctggacggtg cggctatcga tatgcccgca ggcccgtcgg
    35401 aagtgttggt cattgccgac agcggcgcaa cgccggattt cgtggcttct gatttgcttt
    35461 ctcaggctga acacggcccg gactcacagg tgattttact gacgcctgac gctgatatgg
    35521 cgcatcaagt tgccgaagcc gtcgaacgcc agttagcaga actgccgcgt gccgaaaccg
    35581 cccgccaggc actgaacgcc agccgcctga tcgtgactaa agatttagcg cagtgcgtag
    35641 agatctccaa ccagtacggc ccggagcacc tgatcatcca gacccgcaac gcccgcgatc
    35701 tggtcgatgg catcaccagc gccggttcgg tatttcttgg tgactggtca ccggaatcgg
    35761 caggtgatta cgcctccggc accaaccacg ttctgccgac ttacggttac accgccacct
    35821 gttccagcct cgggctggca gatttccaga agcgtatgac cgtgcaggaa ctgtcgaaag
    35881 aaggtttctc cgcgctggct tcgaccattg aaacattggc cgccgccgag cgcctgaccg
    35941 cccataaaaa tgccgttact ttgcgtgtta acgcccttaa ggagcaagca tgagcaccgt
    36001 gactattacc gatttagcgc gtgaaaacgt ccgcaacctg acgccgtatc agtcggcgcg
    36061 tcgtctgggc ggtaatggcg acgtctggct gaacgccaac gaatacccca ctgccgtgga
    36121 gtttcagctt actcagcaaa cgctcaaccg ctacccggaa tgtcagccga aagcggtgat
    36181 tgaaaattac gcgcaatatg caggcgtaaa accggaacag gtgctggtca gtcgtggcgc
    36241 ggacgaaggt attgaactgc tgattcgcgc tttttgcgaa tcgggtaaag acgccatcct
    36301 ctactgcccg ccaacgtacg gcatgtacag cgtcagcgcc gaaacgattg gcgtcgagtg
    36361 ccgcacagtg ccgacgcagg acaactggca actggactta cagggcattt ccgacaagct
    36421 ggacggcgta aaagtggtct atgtttgcag ccccaacaac ccgacagggc aactgatcaa
    36481 tccgcaggat tttcgcactc tgctggagct aacccgcgga aaagccattg tggttgccga
    36541 tgaagcctat atcgagtttt gcccgcaggc atcgctggct ggctggctga cggaatatcc
    36601 gcacctggct attttgcgca cactgtcgaa agcttttgct ctggcgggcc ttcgttgcgg
    36661 atttacgctg gcaaacgaag aagtcatcaa cctgctgatg aaagtgatcg ccccctaccc
    36721 gctctcgacg ccggttgccg acattgcggc ccaggcgtta agcccgcagg gaatcgtcgc
    36781 tatgcgcgaa cgagtgacgc aaattattgc agaacgcgaa tacctgattg ccgcactgaa
    36841 agagatcccc tgcgtagagc aggttttcga ctccgcaacc aactacattc tggcgcgctt
    36901 taaagcctcc agcgcagtgt ttaaatcttt gtgggatcag ggcattatct tacgtgatca
    36961 gaataaacaa ccctctttaa gcggctgcct gcgaattacc gtcggaaccc gtgaagaaag
    37021 ccagcgcgtc attgacgcct tacgtgcgga gcaagtttga tgagtcagaa gtatcttttt
    37081 atcgatcgcg atggaaccct gattagcgaa ccgccgagtg attttcaggt ggaccgtttt
    37141 gacaaactcg cctttgaacc aggcgtgatc ccggaactgc tgaagctgca aaaagcgggc
    37201 tacaaactgg taatgatcac caatcaggat ggtctgggaa cacaaagttt cccgcaggcg
    37261 gatttcgatg gcccgcacaa cctgatgatg cagatcttca cctcgcaagg cgtacagttt
    37321 gatgaagtgc tgatttgtcc gcacctgccc gccgatgagt gcgactgccg taagccgaaa
    37381 gtaaaactgg tggaacgtta tctggctgag caagcgatgg atcgcgctaa cagttatgtg
    37441 attggcgatc gcgcgaccga cattcaactg gcggaaaaca tgggcattaa tggtttacgc
    37501 tacgaccgcg aaatcctgaa ctggccaatg attggcgagc agctcactaa acgagaccgt
    37561 tacgctcacg tagtgcgcaa caccaaagag acacagattg acgttcaggt gtggctggat
    37621 cgcgaaggtg gcagcaagat taacaccggt gttggcttct ttgatcacat gctggatcag
    37681 atcgccaccc acggtggttt ccgtatggaa atcaacgtca aaggcgacct ctatatcgac
    37741 gatcaccaca ccgtcgaaga taccggcctg gcgctgggcg aagcgttaaa aatggctctt
    37801 ggcgacaaac gcggtatttg ccgctttggt tttgtgctac cgatggacga atgccttgcc
    37861 cgctgcgcgc tggatatctc tggtcgcccg cacctggaat acaaagccga gtttacctac
    37921 cagcgcgtgg gcgatctcag caccgagatg atcgagcact tcttccgttc gctctcatac
    37981 accatgggcg tgacgctaca cctgaaaacc aaaggtaaaa acgatcacca ccgtgtagag
    38041 agcctgttca aagcctttgg tcgcaccctg cgccaagcca tccgcgtgga aggcgacacc
    38101 ctgccctcgt cgaaaggagt gctgtaatga acgtggtgat ccttgatacc ggctgcgcca
    38161 atctgaactc ggtgaagtca gccattgcgc gccacggtta tgaaccaaaa gtcagccgtg
    38221 acccggacgt ggtgttgctg gccgataaac tatttttacc cggcgttggc actgcgcaag
    38281 cagcgatgga tcaggtgcgt gagcgcgagc tgtttgatct catcaaagcc tgtacccaac
    38341 cggtactggg catctgctta gggatgcaac tgctgggtcg gcgcagcgaa gagagcaacg
    38401 gcgtcgactt gctgggcatc attgacgaag acgtgccgaa aatgaccgac tttggtctgc
    38461 cgctgccgca tatgggctgg aatcgcgttt acccgcaggc aggcaaccgc ctgtttcagg
    38521 ggattgaaga cggcgcgtac ttttactttg ttcacagtta cgcaatgccg gtcaatccgt
    38581 ggaccattgc ccaatgtaat tacggcgaac cgttcaccgc ggcggtacaa aaagataact
    38641 tctacggcgt gcagttccac ccggagcgtt ctggtgccgc tggcgctaag ttgctgaaaa
    38701 acttcctgga gatgtgatga ttattccggc attagattta atcgacggca ccgtggtgcg
    38761 tctccatcag ggcgattacg gcaaacaacg cgattacggt aacgacccgc tgccgcgctt
    38821 acaggattac gccgcgcagg gtgccgaagt gctgcatctg gtggatctga ccggggcaaa
    38881 agatccggca aaacgtcaaa tcccgctgat taaaaccctg gtcgcgggcg ttaacgttcc
    38941 ggtgcaggtt ggcggcggtg tgcgtagcga agaagacgtg gcggcgttac tggaagcggg
    39001 cgttgcgcgc gtggtggttg gctccaccgc ggtgaaatca ccagaaaggg tgaaaggctg
    39061 gtttgaacgt ttcggtgccg atgccttagt gctggcgctg gatgtccgta ttgacgagca
    39121 aggcaacaag caggtggcgg tcagcggctg gcaagagaac tcgggcgttt cactggaaca
    39181 actggtggaa acctatctgc ctgtcggcct gaaacatgtg ctgtgtaccg atatctcgcg
    39241 cgacggcacg ctggcaggct ctaacgtctc tttatatgaa gaagtgtgcg ccagataccc
    39301 gcaggtggca tttcagtcct ccggcggtat tggcgacatt aacgatgttg ctgccctgcg
    39361 aggcactggc gtgcgcggcg taatagttgg tcgtgcatta ctggaaggta aattcaccgt
    39421 gaaggaggcc atcgcatgct ggcaaaacgc ataatcccat gtctcgacgt tcgtgatggt
    39481 caggtggtga aaggcgtaca gtttcgcaac catgaaatta ttggcgatat tgtaccgctg
    39541 gcaaaacgct acgccgaaga aggtgcagac gaactggtgt tctacgatat caccgcttcc
    39601 agcgatggcc gtgtggtaga taaaagctgg gtatctcgcg tggcggaagt gatcgacatt
    39661 ccgttctgcg tggcgggtgg gattaagtct ctggaagatg ccgcgaaaat tctttccttt
    39721 ggcgcggata aaatttccat caactcccct gcgctggcag acccaacctt aattacccgc
    39781 ctggcggatc gctttggcgt gcaatgcatt gtggtcggca ttgatacctg gtacgacggc
    39841 gaaaccggta aatatcatgt gaatcaatat accggcgatg aaagccgcac ccgcgtcact
    39901 cagtgggaaa cactcgactg ggtacaggaa gtgcaaaaac gtggagccgg agaaatcgtc
    39961 ctcaacatga tgaatcagga cggcgtgcgt aacggttacg atctcgaaca actgaaaaaa
    40021 gtgcgtgaag tttgccacgt cccgctaatt gcctccggtg gcgcgggcac catggaacac
    40081 ttcctcgaag ccttccgcga tgccgacgtt gacggcgcgc tggctgcttc cgtattccac
    40141 aaacaaataa tcaatattgg tgaattaaaa gcgtacctgg caacacaggg cgtggagatc
    40201 aggatatgtt aacagaacaa caacgccgcg aactggactg ggaaaaaacc gacggactaa
    40261 tgccggtaat tgtgcaacac gcggtatccg gcgaagtgtt aatgctgggc tatatgaacc
    40321 cggaagcctt agacaaaacc atcgaaagcg gcaaagtcac cttcttctcg cgcactaaac
    40381 agcgactgtg gaccaaaggc gaaacgtcgg gcaatttcct caacgtagta aatattgcgc
    40441 cggactgcga caacgacacg ctactggtac tggcgaatcc tatcggacca acctgccaca
    40501 aaggcaccag cagctgcttc ggcgacaccg ctcaccaatg gctgttcctg tatcaactgg
    40561 aacaactgct cgccgaacgc aaatctgccg acccggaaac ctcctatacc gccaaactgt
    40621 atgccagcgg caccaaacgc attgcgcaga aagtgggcga agaaggcgta gaaaccgcac
    40681 tggcagcgac ggtacatgac cgctttgagc tgaccaacga ggcatcggac ttgatgtatc
    40741 acctgctggt gctgttgcag gatcaggatc tggatttaac gacggtaatt gagaacctgc
    40801 gtaaacggca tcagtgagtt gcagggtaag cggatgcgat gttgttgtcg catccggcaa
    40861 aaaaaaaggg caaggtgtca ccaccctgcc cgttttcttt aaagctgcaa agattacttc
    40921 gcgttataat tacgcagagc gttacgcccc agcacaatcc ccgcgccaac catgccgccc
    40981 agcagcaccg ccagaatcaa ggtaattgcc tttttcgggc tatcgcgacg aataggtaac
    41041 gtcggtttca tcacataacg gtaagcatga atatcaagat catcaacctt taagttttca
    41101 atatccaaca ggttttgacg tgtctgatag tagtttggtg agaacaccaa cggacgggtc
    41161 gcctcatgct taatcatcga ctccagcgct tcgctcccca aaaggaacaa cgtatcttgt
    41221 gtcacatctt cagtctgctg aacctgtggc tttgccacct gcgcctgatt cgcatactgc
    41281 aacgcttcct gaatctgacg gatacgcaga tctttctgct cctgcgcgac tacttcctgc
    41341 gttcttaaag agtcctgcaa gtttttccgt cccagagcaa tgttgtcttt gagatccttt
    41401 tctagctctt gattcacttt atcatcaact tgctgaatgt attgggccaa cttcatttgt
    41461 gctccctctg cagtttgccc aacataagaa acagtcaatg gtaattgctg gttcttaaca
    41521 gaaggttcga tggtaagttt ttctggttct tcctgattat ccagcgtttc tgctaatgca
    41581 gagaaggcag aactgaagcg accaattaac gtctcctgta aatccgatac tttcggtgca
    41641 gcctgaccat agataacatt catggcattg ttatagccag caatttgccc cacgtcgggc
    41701 tgagtgataa ttgctgttga cgtccatttc tccttcgcta ctgccaaata tccaatagcc
    41761 agggcaatag ccacaatgac ggaaatgatg attgtcatct tgccacgcca caactgcgct
    41821 agtaaatcaa tcaaatcaat ctgttccggg tcatggtttt gcccagaaac attattattt
    41881 tctactctca tcattaccct aactgacgga aatttccaag tccgcatagt ttagcgataa
    41941 tcctagtgaa tgctatagga taagtgataa aaataatgaa ttattgggga ggtaacaaaa
    42001 gacaatcagg gcgtaaatag ccctgataac aagatgttag tcgctgccaa agagatcgcg
    42061 ggtgtatacc ttatctgcca catctttaag ctcttctgcc attcggttag agataatgac
    42121 gtcggcttgt tgtttgaaga tggcgagatc acgttccagc cgagagttga agaatgagtc
    42181 ttctttcatc actggctcgt agatgatcac ttcaacgcct ttcgccttga tacgcttcat
    42241 aatcccctga atagaagacg cacggaagtt atctgaaccg ctcttcataa tcagacgata
    42301 aatacccacc acttgcggtt tgcgtgacaa aatggcatcg gcaataaaat ccttacgcgt
    42361 gcggtttgca tcgacaattg ccgagatcag gttattcggc acagactgat agttcgccag
    42421 taactgcttg gtatctttcg gcaagcaata accaccataa ccaaacgacg ggttgttgta
    42481 atggttgcca atacgcggat cgagacaaac gccttcgata atctggcgag tattcagacc
    42541 taaactttct gcgtagctat ccagttcgtt aaagtatgcc acgcgcatcg ctaggtaggt
    42601 gtttgcaaaa agtttaatcg cttctgcttc ggtcgaatcg gtgaacaggg tcgggatatt
    42661 ttgcttaatc gcgccttcct gtaacaacgc agcaaaacgt tctgcgcgtt ctgaacgctc
    42721 accgatgaca atacgtgaag gatggagatt atcgtaaagg gctttacctt cacggagaaa
    42781 ttccggagag aaaataatat tttcagtacg atatttctta tgcatcgctt cggtaaaacc
    42841 aacgggaacc gttgatttga tgaccataac cgcataagga tttatctcaa ctacgtcttt
    42901 aattactgat tctacactgg atgtattgaa ataattagtt ttaggatcat agtcggttgg
    42961 agtggcgatg atgacataat cagcatcccg gtaagcttca tttttatcta atgtggcatt
    43021 aaagtgtatt ttatctgatt gcaaaaactg ctgaatttcc ttatcaacaa taggagatat
    43081 ccgatcattc agcatagcaa cgcgtgacgg taaaatatct aatgccacaa cctcatgatt
    43141 ttgtgcgatt agaagcccgt ttgacaagcc tacatagcca gtaccggaaa tggtgatttt
    43201 catttcatgc tctcagaatt aacttaactg tgaatcatga tgtttttagc atcctgataa
    43261 gagctaaatg ttttaacgct acgttattag ctggtcaacg ctacgaaaaa caattcagat
    43321 tttctttata agaatagctg atatttatta agttaatatt aagcagtgaa atttagtcta
    43381 taagataaat ggcaaaaaaa aagcccggtg caatatacgc cgggcctcaa ttttactgtt
    43441 ggttaaatca gattaatcca gccattcagt atggaacaca ccttctttat caatgcgctt
    43501 ataagtatgt gcaccgaaat agtcacgctg tgcctggatc aggttcgcag gcagaacagc
    43561 ggcacggtag ctatcgtaat aggcaaccgc agcggcgaag gtcggaaccg ggataccgtt
    43621 ctgtactgca taagcaacga catcacgcag cgcctgctgg tagtcatcgg caatttgctt
    43681 gaagtacgga gccagcagca ggttagcgat ctgcggattt tcggcatatg catcggtgat
    43741 tttctgcagg aactgcgcac ggatgatgca gccagcacgg aaaatcttcg cgatttcgcc
    43801 gtagttcaga tcccagttgt attcttcaga cgcagcacgc agctgagaga agccctgagc
    43861 gtaagaaacg attttgccca gatacagcgc acggcgaact ttttcgatga actcagcctt
    43921 gtcgcctgct gactgagctt gcggaccaga gagaacttta gatgcggcaa cgcgctgatc
    43981 tttcagagaa gagatataac gtgcaaacac agactcggta atcagcgaca gcggttctcc
    44041 gagatccagc gcgctctggc tggtccattt accggtacct ttgttagccg cttcatccag
    44101 gatcacatca accaggtagt taccgtcttc atctttttta gtgaagatat ctttggtgat
    44161 gtcgatcagg tagctgctca gttcaccgtt attccactcg gtaaaggtct gtgccagttc
    44221 ttcgttagag agattcaggc cgcctttcag gagagaatag gcttcagcaa tcagttgcat
    44281 atcaccgtat tcaataccgt tgtgaaccat cttcacataa tggcctgcgc catcagcacc
    44341 gatataggta acgcacggtt cgccatcttc agcaacggcg gcgattttgg tcaggatcgg
    44401 cgcaaccagt tcataggctt ctttctggcc accaggcatg atggacggcc ctttcagcgc
    44461 cccctcttct ccgccggaaa caccggtacc gatgaagtta aagccttcgg cagaaagctc
    44521 acggttacga cgaatggtgt cctggaagaa ggtgttacca ccatcgatga tgatgtcgcc
    44581 tttatcgaga tatggtttga gcgaatcaat agcagcatcc gtgcctgcac ctgctttcac
    44641 cattaacagg atgcgacgag gcgtttccag agattcaaca aactctttca ccgtatagta
    44701 aggaaccagt ttcttgcctg gattttcggc aatcacttct tccgtctttt cacgggaacg
    44761 gttgaaaata gagacggtat aaccacggct ttcgatgttg agcgcaaggt tgcgccccat
    44821 cactgccata ccgactacgc cgatctgttg ctttgacatt gtttactcct gtcagggggt
    44881 ggtcaccgcc gagtgtgcgg cttaaatgtg gtatagatgt taactcactc atagagatgg
    44941 tgaatagcat tacctttgct ataccacact gaatatctca ccttttccgt cttttaaaac
    45001 ttatttatat gaaattactg aattacttgt tcagtaactc aagaataagt tttgtctttt
    45061 cttccatcag cggcacatca ccgcgagatt ccacattcaa ccgcaccacc ggttcggtgt
    45121 tggaggtgcg caggttaaag cgccagtcgg caaaggtcat gctgatgcca tcggtgcgat
    45181 ccaccgccag cgcctcgcgg ctaaaatgct gttccacgcg gttaatcgcc tcaaagggtt
    45241 gcgccagttt gctgttgatc tcaccgcttg ccggaaacgc tgccatccgg tcgcgcacca
    45301 gttcgcccag cgtttttcct ttcaggcaca ccagttcggc gaccagcagc cacgggatca
    45361 tgccgctgtc gcagtaagcg aaatcacgga agtaatggtg ggcgctcatt tcaccaccgt
    45421 agatggcgtc ttccttgcgc atccgttctt taataaaggc gtgtccggtt ttcgacatca
    45481 ccggcgtgcc gcctgcggca gtcaccacat caacggtgtt ccaggagaga cgcggatcgt
    45541 ggatgatctt cgcgccggga tttttttcga ggaatgcttc tgccagcagg ccgacaatgt
    45601 agtagccctc aataaactgc cctttttcgt caaacaggaa gcagcggtca aaatcgccat
    45661 caaaagcaat gcccatatcc gcaccgtgtt tgatgaccgc attgcgggtg tcgtcgcggc
    45721 attccggcag tagcgggtta ggaataccgt tggggaaatt gccgtccggc gtgttgtgca
    45781 ctttgattaa ttccacgggt gcgccaaggg ctttaaagcg ggcttcgatg gcgtccacca
    45841 ccggacccgc tgcgccattc ccggagttaa tcaccagctt gagcggcgtg aggtttttga
    45901 cattgatata accgaacagg tgatcaacgt aagcgtcacg cagattgatt tgctgatagc
    45961 gaccgcgttt ggtttcatca acgggaggaa agtcgttggc ttctgccaga cgctggacat
    46021 cgcgcagtcc ggtatcaccg ctgatcgggc gagccccttc gcgcaccagt ttcatgccgt
    46081 tgtaatccat cgggttatgg ctggcggtaa cttcaatgcc gccatccacg ccgagatgga
    46141 acgtggcgaa atagatctct tcggtgccgg acatgccaat atccagcaca tcgacgcccg
    46201 catcctgtaa acctctcgcc agcgccagtt ttaaggtttc gctggtgagg cggacatcac
    46261 cgcctaacac aatggttttc ggtttgagaa attcgccata ggcgcgacca atgcgccagg
    46321 cgatatcttc attcagttct tcgcctaatt tcccgcgaat atcataggct ttaaagcagg
    46381 ttaatttttt catgtcgtta ccctttttca ggcaatagtt ggccctgacc gaaacggcca
    46441 attttgtttt tgtcgttatt tacatctcgg tttcgcattc attgcctgat gcgacgttta
    46501 cacccgtccg tagcgatccg cgaaacgcac cacatcatcc tcttcgagat aagagccgga
    46561 gcgcacttca attaaatcga gcggaatttt ccccgggttt tccaggcaat gcatcgcccc
    46621 cagcggaata taaatggact cgttttcacc aagcagtttg atatcaccat caatggtgac
    46681 ttttgccgtt cccgcgacaa ccacccagtg ttccgcgcgg tgatggtgca tctgtaccga
    46741 caagccctcg cccggtttca cggtgatgcg tttcacctgg tagcggtcgc ccgcgtcgat
    46801 agagtcatat ttgccccacg ggcgatagac ttcgcgatgc acccgatgct catggcgacc
    46861 atcggctttg atctgctcga ccactttttt cacatcctgc accgcgttac ggtcggcaat
    46921 cagcaccgca tctttggtct gcaccactac cagatctttc accccgacgg tggttacgag
    46981 gccagattcg gcgtacacat agctgttttc agttttgtga ttaatcacat cgccgtggca
    47041 aacgttgccc tcggcggtgt gggcgctaat ttcccataat gaggaccagg agccgacatc
    47101 gctccagccc gcatccatcg gcaccacaac ggcatctgcc gtacgttcca tgaccgcgta
    47161 atccaccgac tcttccgggc aggcgagaaa cgcttcttca tccacacgaa taaaatcgag
    47221 atccggatcg acggcgctca tcgctttttc acaggcatcg agaatatccg gacgatattt
    47281 tttcagttct tcgagatagc gtccggcgcg gaacaggaac atgccgctgt tccagtaata
    47341 ttcgccgctt gccacatagg cctgggcggt ttccagattc ggtttttcga caaactgcgc
    47401 cacttcaaag gccaccgtat cctgctcacc cgcagacact tcaccgcgac gaatatagcc
    47461 ataaccggtt tctggtagat ccggcacaat gccgaaggtc accagcttgc ccgcttcggc
    47521 atatggcatg gcattacgca cggcggcacg gaacgcgtct tcatcggcaa tcacatgatc
    47581 cgccgccaat accagcatta acgggtcgct ctccgggcta tgacgttttg ccgccagcgc
    47641 cgccagcgca atggcaggtg ccgtgtttcg ccctgccggt tcgagaataa tgttctcggt
    47701 aagtttgttc agttgacgca gctgttccgc gacaataaag cggtgctgct cattgcaaat
    47761 caccaccggg ctttcgcact ccacgccgtt caggcggcag atggtggttt gcagcatggt
    47821 gagatcgcct ttcaggcata aaaactgctt ggggtaaagt acgcgggaaa gcggccataa
    47881 gcggctaccg gagccacctg ccatcacaac tggatagagt ttcgactgcg ccataattat
    47941 ccccgaatat catttataaa ttgacgtaac acgttctctt tatcgagtgt gcgttcggca
    48001 tattcacgtg ccaccgtgtt gtgtttgggc agcaggagcg cctgacggat ccccgccacc
    48061 agcgcctcga ccgattccgg ttctacgcaa accgcaatgc ccggaaaggt ttcgcaaagc
    48121 tgccccagtt ctgtgtgggc ctctgcggta atcaccgcgt taccgcctac cgccagaata
    48181 ttggtcagtt tcgacggcaa tacggcatct gccgcgccgc gtttttgcac caccagatgg
    48241 caatcgccca tcttcagcag tgcgggtaaa gcgtcatacg attgcagcgg gaaaaattgc
    48301 atgttgcgca gtccacgctg ctgcgccatt ttttccagcc gcgctttgcc gccgccctgc
    48361 ccgacaatgg caaaaatcag cggttcatcg cgcagacgat cggcagcttc aataacgttt
    48421 tccagcccct gcttttcacc aatattgccg gagtaaagaa tgattttttt gttatccggc
    48481 aggtcaagct ggttacgaag ggcgtcaaca tcggcatctg caacatgctg aaaacgggca
    48541 atttccgacc agttggggaa gaagatgacg ttttccgccg ccacgccttt ttcgatggct
    48601 ttattcatca tcgaacgcga aatcgtggag acgttatcga cgttatgcag tccgctacgt
    48661 tcgaacgccg ttgccagctg tgccactttg ctgcctttgc cttttccggc aaggcccagc
    48721 cccagcatgg cgtccacttc gtaatcctga atatgcagca cggtacgcgc accagaaagt
    48781 ttcgccagca ggcgcattcc cggcgtgcaa aacagcgttg gcaccacgcc gataatgcga
    48841 tccggcttcc agcgacgttg cgccatcaga ggaaagaaac tgctgacggc aaaactgccg
    48901 agatgtaaca ggcgtttcag ggtgctcggc tgttttggca cgtacagcgg gcagcgccac
    48961 accgtggcgg ccccctcttc tcgtttgtac cgccaggcgg aatagttctc gcccacctgc
    49021 cactgcgggt agtaaggcgg tgcggtaata acccgcacct catgaccttg tgccgccagc
    49081 cattccacca tctcgccggt gtatttgccg ataccggtta actccggcga gtagttaatg
    49141 ccgtagacca gtattttcat aatccgggta ctccggcacg cttctcagcg agaaaatagg
    49201 cgcggctgtt agcgtgaaca tcgttactgg cgagcagcgc gtccggcgtc agccagcggt
    49261 aatcgtcatg ctgctcatcc ggcagtatca gctcttcttc agcgactctg aagcgaaaac
    49321 cgagcaccac atagtgagtg gtgaaatccg tgccggagaa gttatcgtca taaaagtgct
    49381 gccagacacc gtaaaactgg cctgctgtta tcggcagacg cagccccagt tccgccatcg
    49441 tcagccgctc gaatgcggct tccagcgttt cgtctttctg cacgcgccct cccggcacaa
    49501 accagtaacc ctgcgccggg cggttggttc ttttgccaag cagaaactca ccgcgactgt
    49561 tctcgacaat aaagtcgaga gagacaagcg gggtggagcg cactaccgtg gcaaagtctt
    49621 cctgacgtaa aaacatcatt acccccgaaa gcggtcttga ttctcaagga accactggta
    49681 agtgctggca agccccgctt ccagtgagat ttcgtgatac cagccaagct gatgcaggcg
    49741 cgtcacatcc agcagtttgc gcggcgtacc atccggtttg ctggcatcaa aaaccacccg
    49801 acctttgtaa cccaccactt tggcgatggt ttgcgccagc tcgcggatag tgcagtcaac
    49861 gcccgtgccg acgttaatgt gcgacagcat cggctgggtg ttctccagcc agacttcgtg
    49921 cgccagctcc atgacatgaa tgctcgccgc cgccatatca tcgacgtgca ggaattcacg
    49981 catcggtgta ccgctgcccc ataccaccac gtccggtgca ttctgtgccg tcgcctcgtg
    50041 gaagcgacgc agcaatgcag ggatcacatg cgaattactc gggtggaagt tgtcatgcgg
    50101 gccatacagg ttggtcggca tgactgagcg gtaatcgcgt ccgtactggc ggttgtatga
    50161 ttcgcacagt ttgatcccgg cgattttggc aatggcgtaa ggctcgttgg tcggctccag
    50221 cgtgccctgc aataactcgc tttctgccat cggctgtttt gccagtttcg ggtagataca
    50281 ggacgatcca agaaacagca gtttgttcac gtcgttctga tgcgcggcgt gaatgatgtt
    50341 gctctcaatc atcatgttct ggtagatgaa atccgccgga taggtgttgt tggcaacaat
    50401 gccgcccact ttcgccgccg ccagatagac ctggtcaatg cgttcgctgg caaagaaatc
    50461 atgcaccgcg cggctgtcca gcaggttcag ctcgtcgcgg gtgcgtaata ccagttccac
    50521 atcaccgcgc tgttcgagct gccgcctgat ggcagaaccg accatcccac gatggccagc
    50581 gataaaaatg cgttgtttac tcatgcttat gactccagcg cgatcgccac gtcgtagccg
    50641 tgagatttca gcagagagtg ttttttcgcc gcttcgaggt cattagccac catttcagac
    50701 accatctctc tgagggtgat ttccggtttc cagcccagtt tttcgtgcgc tttggtcggg
    50761 tcgccgagca gcgtttcaac ttcagccgga cggaagtaac gcggatcaac ggcgataatc
    50821 acatcacccg gtttaacgcc cggcgcgtca tgcccggtga cggaaaccac aatgcccttc
    50881 tcttcaacac ccgtgccttc aaagcgcagt ttgatgccca gctgtgccgc tgccatttcc
    50941 acgaactgac gcacggagta ctgaacgccg gtcgcgataa cgaaatcttc cggctgttcc
    51001 tgttgcagca tcatccactg catttttacg tagtctttgg cgtggcccca gtcacgcagg
    51061 gaatccatat tgccgaggta caggcacgac tccagcccct gggcgatgtt ggcgattgcg
    51121 cgggtgattt tgcgggtaac gaaggtttcg ccgcggcgcg gggattcatg gttgaagaga
    51181 attccgttac aagcgtacat gccgtaggat tcgcggtagt taacggtgat ccagtaggcg
    51241 tacagtttgg cgaccgcata cggagatcgc gggtagaacg gcgtggtttc tttctgcgga
    51301 atttcctgca ccagaccata cagttcagag gtggaagcct gatagaaacg agttttcttt
    51361 tccagaccga ggaagcggat cgcctccagc aggcgcagcg tacccatcgc gtcgacgtca
    51421 gcggtatatt ctggtgactc aaaagagacc gcaacgtggc tcattgcgcc caggttgtac
    51481 acttcatccg gctgtacttc acgcaaaatg cgcgtcaggt tagaggtatc actcaggtcg
    51541 ccataatgca gatggaattt cgggttgcag gtgtgcggat cctgataaat gtgatccacg
    51601 cgctcggtgt tgaatgacga tgcgcgacgc ttaataccat gcacctcgta acctttttcc
    51661 agcagaaact ctgccaggta agaaccgtct tgtccggtta caccggtgat gagagcgact
    51721 tttgacatgt cttattcctc tgtatttttg aatttattca gtttcaacgc gttcgcgtat
    51781 caccactgcg ggattccccc ggcaaaccac atttgccgga agcgatttaa aaacactgct
    51841 tcgtgcaccc acgacggtgc cgtcgccgat tgtgacgcct ggggcaacaa agacatcggt
    51901 tgccagccag catttctcgc caatcacaat aggcgtggcg ttaatggtga aatgttgact
    51961 tgcatggtcg tggctaccgg tgcataaata acttttttgc gatatcaccg aatgtgcgcc
    52021 aatggttatt tcaccgaggg tatataaatt gacgtcatcg ccgacccacg cgtaatcacc
    52081 taaggttaat ttccacggat aggtaatttt tactgacgga cgaataacta cgttttttcc
    52141 tatttttgct ccgaataaac gtaataaaaa agcccgccag cgatacaata cttgtggcga
    52201 ccaggcaaat attgttgcct gtactgccca ccataattgc actttaatag cgttgccgcc
    52261 ccggaaccct ttcggcaccg agaaaccgct taaatcttgc attggttttc cttatattca
    52321 gactttgtta tataaggctt tcgtctttga ggtagtacgt tggcgtaaat gccaggataa
    52381 ttcagcccaa aagccaggca catgtaatat ttgtcgttgg acttttttcg cgtcagcaca
    52441 caattccata ttattggtgg tagatacccc acccatggaa aattcagaca ccaggccatt
    52501 gagtttttta aatgcataac cggctttata cattttggct gccagcgcgt agtcggaaga
    52561 aactttatat tccaggtcat aacgccattt tttcaagccg gatacaggga aaaatatcgc
    52621 ctgatgactg gcgggcaggc tgtgataaat ataccagccc ggtttggcgc tacgtttaat
    52681 tttatgcccg tcgccaaaat ccagcagcgc atcgccggtg atcatcatgt tatctttttg
    52741 catttttaac ttacggacaa aatttgcggc atcctgatga aaaatatcgc ccgaattgag
    52801 aaacaacgcg aacttgcctt gtgccatcgt aatgcctttg ttcatggcgt cgtagatacc
    52861 gttatctggc tcgctgacaa agcgtaggtt atagatacca ttgagatttt ccagatactc
    52921 acgggtgccg tcattggaac cgccatcgac aacaatccat tcgaagctga tatcttccgc
    52981 ctgcgccaga tgcgccagcg aggcatgtgt tttgactatc ccttcgaggt tacgaaacgc
    53041 gacagtgatt atgctaagca acatgttctt attcttacct cgtaatattt aacgcttttc
    53101 gcaaaataaa cggacagacg attaaaaatg catattccgg gctaaatatc gaaccggtaa
    53161 aaaacagcga taccggagta aaaagatata attgcacgcg aaaattgcgg ttatcaccaa
    53221 aagcgttgat catcattttt atcactttcc ccatgtacca cagcgataaa aacactgcga
    53281 accaggaaaa ataaataatc agcagataca aaccattgtc tatggttttt ccgacatccg
    53341 caccgttaaa tattccgaat gatgcgacgt attcataaag tgagccaaat ctgactacac
    53401 cgtcaatatg ggtcaaagaa taaccgacca tcaccagcgg accgacaata cgataatacg
    53461 atgacgatcc ttccgtacct aaatcgccca gacgggtgga aatataagga aacgcgatta
    53521 ccacaccaac ccggaataca gccagagaaa tcaatgccaa cggtaacttt ttcttaatcg
    53581 cctctttatt cagatattga aatgcccact ccagcaaata aaacaggata aaagtcataa
    53641 cccctgaaaa cgatccggat aatattatcc ctgcgagaat catagcatcg gttttaggcg
    53701 ttttgatacc aaactgtttg atgctgagcc aaattgagat taatgccaga gcgaaaaatg
    53761 ccggttcgaa ataaagtgct gtcgtgcgct tgccgccgaa tttaatgaaa ttcagtacat
    53821 agctgttgct gtaaatcaga tatttcgaaa tcgactccat aatactgctg ccaccggtga
    53881 ggataatttg cgccatctcc accgccgcca gcgccaccac cagcccgatc accagataaa
    53941 agaaacgtaa tatcttgcga tggttgtgtg gcgaaatcgt tttaaagcga atactccaca
    54001 ccatgccaat aatgatcaca atatagacaa acagcatggt tgaagtgacg tatttactgg
    54061 catccagcga ctgaccaaac agatagttaa acgccgtgag tcccgcacca atccctaagg
    54121 caatcatcaa ttttttaacg ctgatgcgtt ctaaaaacag cagtaacaag acaggtaaaa
    54181 aagtgacgat ggtgatgggg aaactttcgc caagctgggc gattttgacg ttaaccagca
    54241 ggtagatcag cggcagcagc aggtagctac agattctgat agaagttgac atactcctcc
    54301 agcatctgtt gtccactgta ggtggcgcgg ctgcgttggc tgaactcagc cagcgtggta
    54361 ccaaatatcg cctgcgcgat ttccggtttg cttaactgca ccagttgcag cacgtcttct
    54421 tcgtcgacgg ttttaccgcc ggatttttgc aacacttccc gtgccgcgtc gctatgggtg
    54481 gcaatcaccg gcacgccaat tgataacgcc tcacacaaaa tcagcgggta gttatcgacg
    54541 cgagaactga ataccagcgc atccatctga ttgagcgcgc tcatcaactt gcgcttgtca
    54601 gtttcaaagc cgtgattaac cacgttgcca gcggtgaacg gcgagaactt accaaaggta
    54661 tgcagttcga ttttgtcgcc cagcgccatc atctcacgca ccagttgctg gttagtcttg
    54721 ccgtcgtaac gcaagtcatg cgccaccacc gcgattttcg gcttgccctg ggtttcgcgc
    54781 accggaggca agtccgccag aatcgcttcg gttgccatat caataccatt attgataatc
    54841 cggcaacgcc ctggaccgta caggctattg aaagcgtcag ccacatgctg gctgggggaa
    54901 ataaactgac agcccagcgc cagcatctca cggaataact ggcgtttgcc cgccaccagc
    54961 tggtgtgcgc gatcaatctt caccggcgga tagttattta aggtcgggca tttctggcag
    55021 cccgttttcc agccttcgca accgtcggta aaggcgcagc gtccggtaac actccagtgg
    55081 tcgtgcagcg tccagaccag agtgacgtcc ggtttatggt ttttcacttt ttcgcaaaag
    55141 cgcaccacgc tcttaagatt tagccagtag ctgtgcagca catgaaaatg caggaccacc
    55201 ggacccggtg tgcgagtaat ggtgcgatat aactcattga aattgccaaa cagatcgcga
    55261 ttaaacagac gaaacagggc aatattcgcc atcgcggtca tccgcggcgt atgtttgatg
    55321 acctgcggat agttctgatg gctgacgctc tctttgccgc ctttgccata accgtagaca
    55381 aaatgtgacg ccagcccctg ttgcagcgca cgctggtgga gatctaacgc cacacctgct
    55441 gccccgcctt ccgccagtcg cacattaaat tgcagaatat tcatttaatt accttcactc
    55501 gcgctttttc tcccaccacc agcgcgttgt ccgggacaga gtcgagcacc acgctgcccg
    55561 cacccacggt gacgttgtta ccaagcgtga tatcaccaag aataatgacg ttggcaccga
    55621 gttcgacgcc gttgccaatg tgtggacatg ccatgttatc ggcaccacga ttgccgatag
    55681 tgacgccgtg gcgaatggtg aaatcatccc ccgctaccac gtttttattg atcactacgg
    55741 cgtaaccgtg atggatagta aagcggcggc caatggtcgc ggcggcctgg atttcataac
    55801 cgaaaaagca ttcggtgata atgcgataca gcaccagcag cggggccgcc cacagattgt
    55861 tgaggacgtt ctttttgcgc cacaccgaac aaaaatgagc aacacgatag gcaagaacca
    55921 tgcagcacgg gcgtaaactc cagctgttgg cacgcagatc ttccagcatc tttagcgccc
    55981 ccgaatacca tcagccagac gtttgccgtt acgcaccgac agcagcgtca gcaaagtgcg
    56041 ccaggtcata cgtttattgc ggatctggta gagggtaaac agctgatatt ttttgcttgc
    56101 acggtcaaat ttatctttgt gctttcggta aaagtggaag tacccggaga attttttcgg
    56161 tgacgaggtg atctgcattt caccgtgatt gatatgcagg atctgcgtcg cttcttccac
    56221 tttccacggt tcgccgtact ccaccaccat ccgcaggaag atgtcgtaat cctgtgctgc
    56281 tttcagttcg gtatcgaaca aacactcttt gaaacgccat gcccaagtaa agacctgatt
    56341 gccaatgata ttgcgtttat agaacaggcg gcgtgagtac ggcgatttgg gatacagtgg
    56401 taagcttgcc ggttgggaat aaacttctcc ctggcatacg tagtcgttag cgtacaaaaa
    56461 ggcgtgagtg accagttgct gtttatgggc gaggaagacg ctcagacggt tgggtgtcca
    56521 ttcgtcatcg tcatcgatcc cggtaatgta ttccccttgc gccagcataa tcgcctggtt
    56581 acgtaccgcg cacgccccgc tgttaatgtc gttgtgaatg taggtgatac gcggatcgtt
    56641 gagggcagtg acgtactgtt gcagttgttc ccaggaggtg gagcaatcat ccacgatgat
    56701 catctcccag ttgctgtagt cctggcgcag gaccgatttt atcgcccgaa tcgccagttg
    56761 ttgacggttc caggtcggca tatagattga aatcagcgga ttctctttca taggttgttc
    56821 tcccggctta tgagctggtt tgcttgccgg atgcggcgtg agcgccttat ccggcctacg
    56881 gggcggtgcg aatgcaggcg tcgttatttc gcatccgact tatattcgta ttcgtaatag
    56941 ccataatcct gatacgcgct ggcgcggcgg aagatggagt tcagaatcac ccctttcacc
    57001 ggaataccgt tttgctcaaa gcggctcaga ctggtttcca cttctttcaa tgtgttgacc
    57061 gcatagcgcg ccaccattaa cgtggttcca gcgtgacgcc cgacaattgc tgcatcggtc
    57121 actgccagaa tcggcggcgt atcaatcaac accaggtcgt agtttttact cgcccagctc
    57181 accaattcgg caaagcgttc gctcatcaac agttcagaag gatttggcgg cacctgaccg
    57241 cgcgggatca ggtcaaattt ggcaatagag gtcggtttag ctgcggtagt aatatcgccc
    57301 tggccaatca gaatttccga caggccattg acgttattgg tacccaacag ctcgtgggtg
    57361 tagcctttgc gcatatcgca gtcgatcaac aacacccgtt tattggtctg actgatcacc
    57421 gccgccaggt tggcgcagac aaaggtttta ccgattgacg ggctcacccc tgtcatcatc
    57481 aacacattgt tctgcgcctg catcatcgcg aagtgcagac tggtacgcag actgcggatg
    57541 gcttcaatcg ccagatcggt tggattcccc accgccagta gctggctctg tttatagcgt
    57601 ttaatccctt tgatggtttt gacgctatcg cgcgctttct gccattccga cagcgggatg
    57661 ctggcataga cgctgatacc atgttcttcc agcacctgcg ggctttcgat gccgcgatta
    57721 aacaacgagc gcagcagcac acccacgata gagagcatca ggccgaggat aattgcgccg
    57781 aggataatca gtcctttctt cggtttcagc acgccaggct gggtgattgc cgggtcaaca
    57841 atgcgcacat cgccgacggt gctggcttcg gtgattttca gctcctgctc tttattcagc
    57901 agttgcatat agacctgctg accagactcg acatcgcggg tcaggcgcac aatctcctgc
    57961 tgggttttcg gcatcgccgt tacgcgaccg ttaagtttgg ctttctcttc ttccagcgcc
    58021 tgacgtttct ccagcagcgt gcggtacgcc gggtgaactt tggtgtacag cttggagatt
    58081 tccgcctctt taaaggtcag ttcgttcaac tgcgcgtcga tgttcaccat cgaatcgagc
    58141 accgctttcg cttccagcgg cagatcaaca gaatctttat cctgacggaa ggcattcagt
    58201 ttgttttcgg caacatcgag gcggctacgt acttccggta actgttgcgc gaggaaagcg
    58261 aggcttttcg acgcttccgc cgatttgcgc tcaatatttt gttcctgata gttacgggcg
    58321 atgctgttaa gaatgtcgcg gatctgttcg cgatcttcac cggtataagt caggctcagt
    58381 acgcctgcgt ctttgccgtt ctccgttacc gtcaggctgt tttgcagttg gttgatcatc
    58441 cccaacgtgg agtatttggt gacggtaaac tcactgcccg ggcgggcgtg aatggcttca
    58501 accatcagcg tgacgccttc ttttttcagc atctggcccg cttgcccacg ggcgctaaag
    58561 ccgccatcac tgctcagggt gtagttttta ttgtccagca cattaagcgt aaacacctgg
    58621 cccgccatct ctttcgggcg gttaaaggta gtcactttca ccgtctcgtt ctggcgtccc
    58681 atcagtcgct cccagcccgc cccgaaaatc gggaacgtgt ttttgctcac tgcaatatcg
    58741 aggtcgagat cgtccaccgt tttaccaagc accagacgcg aacgaatcaa ctggatctcg
    58801 gcgtccgatg caggcggttt gttggctaat gccgaaccaa tgtcctgcac taacgaattg
    58861 ccgctgtttt gctcgatttg taccagtgcg tcggcactat aaatcggcgt tgcgaagaag
    58921 gtgtaaacca cggcacagag ggcaaataca gcggtgatgc caatcaccca ccagcgcgct
    58981 tcaatgacgg tgccgaccag gcgaccaata tcgatttcat cactgcccgt taccggagcg
    59041 gcatgttgtt ttactttttc tgtcattctt atacctgctc tgcgttcaat gcctgcgccc
    59101 actggcgggc agaccgttca agtaatgtgt acaccgctgc aaacgtttcc cggcttttgc
    59161 gatacggatc ggggatttca cattcgttat cccagtgacc aaacagcatc actttgccgc
    59221 gcatctcagg tgccatctcg cataagcgtt cgatatggcg cttttccatg gtcaaaatca
    59281 ggtcatagtt gcgacacagg cggcggctga tttgtcgtgc acagtggcct tccagagaca
    59341 gttgatgttc tgcggcgacg ctgatagcgg taggatcagc gcccttaccg accagcgcgc
    59401 cgagtccagc ggactccact ttcagttccg ggtgataacg ttgcagtaag cgttccgccg
    59461 tcggggaacg gcaaatattg ccgacacaga caactaatat gttgttaaac atgacgatta
    59521 ccagttatga atgtcgctgg ctgtatccgt catgtaacgg acaccgctaa tagttggcaa
    59581 caactgattg atcagacggt tccagcggga aaccggggcg gtggtgacat acaccacgtc
    59641 ataaggttgc aggcggaatt ctgtcgccat caccagcgac gtggcatcgg acatatccag
    59701 ctggtagata ttggcaatct tgccattacg cccgccctcg cctttcagcg gacgaatgac
    59761 aaagatgccg ctggcgttgg aggtggtcat gtcgatacct tcagcattac ccagggcttc
    59821 agtcagggtc atgccgctaa agtccatttt cagggtgctc tgtttcttca cttcacccat
    59881 cacaaatact ttcagatcat cattacgcgg cacgtagaga atatcgccgg gataaagcag
    59941 tcggttctga ttgaggtcgc cgttttgcat cagcgcctgc aaagaaatgc gttcttcacg
    60001 accattgtgt gttagcacta cgttgcgcca gtcagcggtg tcggtcaggc cacctgcggc
    60061 gttgatggcg tcgagaatag tcagtggcac gttggtgatc gcctgttgac cggatttatt
    60121 cacctgaccg gagatatagg ccttttgtga gcggaaggcg gcgatattaa cgtccacctg
    60181 cgggtcagcg atgtacgtcg ctaagcgccc ggtaatatca ctgcggattt cagcgagcgt
    60241 tttcccgact acgtgaacct tgccgatata cgggtaaaac atagtgccgt caggctgtac
    60301 ccagttgccg gtgtcgctgg agctgcggta ctgaccggct ggcgtggtga gttccgggtg
    60361 atcccagacg gtgacattca gaacgtcgcc cggcccgacg cgatactggt aattcgcgat
    60421 ctcgctttcc agcgtcatat tggggcgcgc tacattcggg cgtgggcgta attggtcaat
    60481 caggcgcggg gtcagcggat aaacattcac cattttgtcg agatcgaaat cagcgtcctg
    60541 ctgtttgatg acgtctttgc ccatcgtcga catattgctg cccggaagta ctgtgcaacc
    60601 gcttatcaag gttactgaca ccaataatgg catcaatttc attttggatt tcatcattgt
    60661 ttatttatca ctttggcaga gtaattatcc tgtgcactat taatagcaat atcgccatgc
    60721 acatttacct tgcagttaat tgaataaaaa tttaactggc atcagtccta aaaaaattga
    60781 tttcatccgc aggctattga cagaataatt cagactggtc tttcaggcat ccagacacgc
    60841 taccgcccct ggctttttag ccaccaacac actgatttag cttaattttt caaaccctct
    60901 ctgcatacaa tcgttgatga gaaagggtta ttgcggaaat taacttccga atataaggtg
    60961 acattatggt aattgaatat tggctctcca ataatgcagg aggaagtgtt acagctaacg
    61021 gaatagcagg caaggtaaca attcggcaat tggctatttt taagaattat attaagtgtc
    61081 attcaatatg gtttttagga gtttctttag gttgacaata tttaatattg cgcctccaca
    61141 tgcgatattt cttaaataat gttttattat taccactttt aattcaggga taatgtgagg
    61201 ttattaccct caaataatat gagataaata gtgctgcaac attgcatttt tgccccgatt
    61261 tatccatgat cgaattgtga catttgtcat acaacgaata ggttttgtac ttactatgga
    61321 atggattgcc gatccgtcta tctgggccgg gttaatcacg ctgattgtga tcgaactggt
    61381 cctcggcatt gataacctgg tctttattgc catcctcgcc gaaaaactac cgccgaggca
    61441 gcgcgaccgc gcacgggtta ccgggctgct gctggcgatg ttaatgcgcc tgttactgct
    61501 ggcgtcaatc tcctggctgg tcaccctgac tcaaccgctg ttcagcttcc gctcgtttac
    61561 ctttagcgcc cgcgacttaa tcatgctgtt tggtggtttc ttcctgttgt tcaaagccac
    61621 gatggagctg aacgaacggc tggaagggaa agacagcaat aatcccacac aacgcaaagg
    61681 tgcgaagttc tggggcgtgg tgacgcaaat tgtggtactg gacgccattt tctcactcga
    61741 ctcagtgatt accgccgtcg ggatggtcga ccatttactg gtcatgatgg ccgccgtggt
    61801 tatcgctatc agcctgatgt tgatggccag caagccgtta acgcaatttg ttaacagtca
    61861 cccgacgatc gttattctct gcttaagctt cctgttgatg attggcttta gcctggtggc
    61921 agaaggtttc ggcttcgtca ttccgaaagg ctacctgtac gctgccattg gtttctcggt
    61981 gatgatcgag gcgctcaatc agttggctat ctttaaccgg cgacgttttc tttccgctaa
    62041 ccagacgctg cgccagcgga ctaccgaagc ggtaatgcgc ctgcttagcg ggcaaaaaga
    62101 agatgcggaa ctggacgccg aaaccgcgtc catgctggtg gatcatggta accagcagat
    62161 ctttaatccg caggaacggc ggatgattga gcgggtactt aatcttaacc agcgtaccgt
    62221 cagcagcatt atgacgtcgc gccacgatat tgagcatatc gatctcaacg cgccggaaga
    62281 agaaatccgc cagttgctgg agcgaaatca gcatacgcgg ctggtggtta ccgacggtga
    62341 tgacgcagaa gatttgctcg gtgttgttca cgttatcgac ctgctacaac agtctctgcg
    62401 cggcgaaccg ctcaacctgc gggtgttgat tcgccagccg ctggtgttcc cggaaacctt
    62461 gccgttgtta cctgccctgg agcagttccg taatgcccgc acgcactttg cttttgtggt
    62521 ggatgagttt ggctcggtgg aagggattgt gacattaagt gacgtcactg aaaccattgt
    62581 cggtaactta ccgaacgaag tggaagagat cgacgcccgc catgatattc agaagaatgc
    62641 ggacggttcc tggacggcga atggtcatat gccgctggaa gatctggtgc aatatgtgcc
    62701 gctgccgctg gatgaaaaac gtgaatatca caccattgcc gggctgttga tggaatattt
    62761 acagcgtatt ccaaaacctg gcgaagaagt tcaggtgggg gattatttgc ttaaaacgtt
    62821 gcaggtggaa agccatcgcg tgcagaaggt gcagattatt ccgctgcata aggatggcga
    62881 gttggagtac gaggtgtgat ggtatcggtc attttaccca atagccgaaa ggaataagat
    62941 gagggaaccg cgccagcggc gatttaccgc cgggagcccg ggttgccagg gtggtggcga
    63001 ttgagccacc ctggcacgtt caccggcttg agcgttccat agtagcgagg aacatcaagt
    63061 gaacggaaca gccttcgatc ttacatattc agaatataat gatgccgcca cgaacgcctg
    63121 atgcgacgct ttacgcgtct tatcaggcct acaggactgg tcacctggta acctacatct
    63181 tctccagcaa cttcttcaca tccttgccat tgcgggaatc tttattccgc tcggcccagt
    63241 cattcaggcg acgtttcgct tcgtcctgta gatgtttgcg cagtaactga tccacttgca
    63301 ggctgtaatt gagttgctgc cagttgccat aaacccgcag cggtactggc gtttctttca
    63361 ggaaatcaat cagtttactt tccccgttcc agccacccac gacccgaata tcaaactggg
    63421 tatcgcaggt ttgatctgcc agattcaaca tgccttcccc tgtcagcgcc agcacaggcg
    63481 attgaccttg catgtcgttt aacgtcacga cgccatcctt caacgtcaaa tcggtggtaa
    63541 agcggtcaag ccgcgtcacg ttatcaaagt tttcagcggc cttcacatca ccgccattac
    63601 gctctaccgc ttgctgaatc atctgctgga agttcatccc ttccatgcgc gtgtcggtca
    63661 tttcgacatg tgcctgtcct tgccagttgt ggcggaatgc gtcggcatct atgtcagcac
    63721 cggagaaatc accagccagt gacatttttc cggtcaacga aatcggatag ttaaacgcct
    63781 tcagaatggt accaatctca acgttttcca gccgtggctg gaagtttatc cgcggattta
    63841 ttgatgtcgc gtccagcgtg cccggcagtg aaacctgtcc accgttaagt ttgccctgca
    63901 gttgagtaat ttccagcaaa cccgacttgt tggtcatttg cgtggcaaca tctgtaaaat
    63961 tcattccgcg ccagcgcacg ttactggcct gcaacaaaat atcagccgta aagccttgca
    64021 gtccctgata ggccggttca tcaatacgcg aagaaatgac cgggcgcggc aacgttgatt
    64081 ggctctgccc ctgctgcgcg gcaccgtttt caccattcgc ggtttcatta agcgggatga
    64141 ggttgtcaag attcagttgc gggaattgca gtctcagctg ccattccggt ttctctgtca
    64201 gcgtgacctg tgcttgcccg ctcagcgtac tgtcattggc ggtcaagctg atttggttaa
    64261 atgaaaggcg tttatgtgac tcctgccact gggcctggaa actcccctgc ccctgaatac
    64321 cttgttttgg taaatcggca ccctgcaact gccagttaat ttgttcaata gccgccgtta
    64381 aatcatgcgg ataatcagaa gcatccaccg taccgttaag ggatattgtg agatcgcgct
    64441 gatcgcgatt aacccgcccg gagaactcaa atgagccacg atgttgggga tcttgttcca
    64501 tttgcaggcg gatattgcgg attgtcacct gctcgtcatc ttcatgctgg aacaccagca
    64561 cgctgtccgc caccttaaga ctggatatat caaacgacca tccgcgatcg tctgacagat
    64621 ccggcaaggt attgtcgcgc ggtgcaaccg gagcgtcttc actgcgcacc gcttccgtct
    64681 gcggcgtcag ttggatcact gcccctttta gcatcacctg cttaacgctc agttgatgac
    64741 tcagtagtgg taaaagcgcc acgtccagac gcatgttgtc ggcgcgaacc agtggctggc
    64801 ttgcgccctg ggcggtgaga gacattcgcc cggagaggat actaagctgc ggccagacgt
    64861 gccaacgcag tggcccgtcg agctgcaatt gataaccgct acgtgcagca acttgcttga
    64921 ccatatagtc gcggaaatca ttcggattca ccagcaacac taacacagat aacccggcca
    64981 ccagcacgac caggagtatc atcagcgtcg tcagaaatcg tctcatggta tcctcaatgg
    65041 gcctgaatta gtctttatcg attcggctgg ctaccgcgcc ctgctggttg cgatatttcg
    65101 catcttcacg gcggttgtaa ggtcgcgccg ccgggccgga aagcggctca aagctcagcg
    65161 caccaattaa catgcccgga cgcagcgcca gcggcagctt accggagttg tagaactcca
    65221 gcacaatgca accagaccag cccggatcga tacggtgcgc ggtgacgtgc accatcagcc
    65281 ccagacgcgc caaagaggaa cgcccgtcca gccagcccac cagatcggct ggcagcgtca
    65341 ccgactccag cgtcaccgcc agcgccagct ctcctgggtg aagatagaac gcctcgccct
    65401 cgtcgagaac gatctcatcg ctcatcacgc ggtcaagcgc ggcgctcact tcatctttgg
    65461 gaccgctcag atcgataaac gctgccgtgt gaccacggaa ggtacgaaat ttattgccca
    65521 ggcgtacatc caccgtcgcg ccgttaatac gctccactgg cggacgtggg ttgatcgaca
    65581 aacggccttc atcaagccag gcttcaatat ctcggtcaca cagacgcatg gcattttctc
    65641 ctttcgcgca tcactccctt aacgccaatt acgtcaaggg cataccaggt tatcactgaa
    65701 cggtacacaa tttatcaagc ttattcaaag aactgactta ttttcgcttt caatatatcg
    65761 atcgcgatgc ggtttttccc gccgcgcggc acgataatgt ccgcatattg tttagaaggc
    65821 tcaatgaatt gcaggaacat cgggcgcacg gttttttgat attgcgccat cacggaatcc
    65881 attgaacgcc cacgctcgtt aacgtcacgc ttgatgcggc gcatcaggca gatatccagc
    65941 ggggtatcaa cgaaaatgga gaagttaagt tcgtcacgca aacgcgcatc cgtcagcaac
    66001 aaaatgcctt cgagaatgat gaccttcttc ggctcaaccg tcaccgtttc tttcatacgc
    66061 gtatgttcaa catagctgta aaccggcagg tcaattgccg agccgcgttt caacgcttgt
    66121 aaatgctcaa gcagcaggct gtgatccatc gcgctgggat ggtcgtagtt ggtcttaacg
    66181 cgttcttcca tcgacagatg gctttgatct ttgtaatagc agtcttcggg aattacgccg
    66241 atgtgttcat caccgacttg ctcacgtaat tcacgataaa gggtactggc aataagactc
    66301 ttgccggaag ccgatgcgcc agcgataccg ataatgacgc actgatgaga ctgatcagtc
    66361 atatatttag cgacctgatt aacctggatg ttaggaaggg ggcgacgaag cgccaaacgc
    66421 ggcaattata gggatttcat ccgtctgata ccagtcgaat agcgttgccg cgcgctcaga
    66481 gttaattgtt gacaaagaat tcccggtggc aaattacgtt gatcagtttt atgtaaggta
    66541 aaaaatgtta tacgcagttg cgcaaattat ccgcctttac gtcactttat gagcaattcg
    66601 catataaaat gtaaaacttt tgtactagca taaacacaga aacgaatact ggcgacctgg
    66661 tcttgcggat aaagcggtaa tgagcaaaca atcacagcat gtattaattg ccctgcccca
    66721 cccgctgctt cacctggtca gtttaggttt agtctcgttt atctttaccc ttttctcgct
    66781 tgagctttcg cagtttggca cccaactcgc cccactgtgg ttcccgacgt ccatcatgat
    66841 ggtggcgttt tatcgccatg ccgggcgcat gtggccggga attgcgctga gctgttcgct
    66901 gggaaatatc gccgcatcca tcctgctttt ttccaccagc tcgctgaaca tgacctggac
    66961 gaccatcaat attgttgaag ccgtggtcgg ggcagtgctg ctacgtaaat tgctgccgtg
    67021 gtataacccc ttgcaaaatc tggctgactg gctgcgtctg gcactcggca gcgccattgt
    67081 tccgcctctg ttggggggtg ttctggttgt cctgctgacg cccggagacg atcctctcag
    67141 ggcatttttg atatgggtac tgtcagaatc catcggcgct ctggcactgg tgccgctggg
    67201 attgttattt aaaccacact atctgctgcg ccatcgcaac ccacggttgc tttttgagtc
    67261 gctgctcacg ttagccatca cactgacgtt aagctggctt tcgatgctgt atctgccgtg
    67321 gccttttact ttcattattg tgctgttgat gtggagcgcc gtgcgcctgc cacgaatgga
    67381 agcctttttg atcttcctta ccacggtgat gatggtgtcg ctgatgatgg ccgcggatcc
    67441 ctccctgctt gctacgccgc gtacgtacct gatgagccat atgccgtggc taccgttttt
    67501 gctgatcctg ctgcccgcca acatcatgac catggtgatg tatgcctttc gtgcggaacg
    67561 caaacacatt tccgaaagcg aaacccgctt tcggaacgcg atggaatatt ccgctatcgg
    67621 tatggcgtta gtgggcaccg agggacaatg gctgcaaacc aacaaagcgc tctgccagtt
    67681 tctcgggtac agtcaggaag agctgcgcgg actcaccttt cagcaactga cctggccgga
    67741 ggatctcaat aaagatctcc aacaggttga aaagctgata agcggtgaaa taaacaccta
    67801 ttcaatggaa aaacgctact acaaccgcaa tggcgatgtt gtctgggcgt tgcttgccgt
    67861 ctcactggtg cgccacacgg atggcacgcc gctctatttt atcgctcaga ttgaagacat
    67921 taacgagcta aaacgcaccg aacaggtcaa tcagcaactg atggagcgca tcacgctggc
    67981 taacgaagcg ggcgggattg gcatctggga gagggagttg aagccgaatg tttttagctg
    68041 ggataagcgg atgttcgagc tgtatgaaat tcctccacat atcaaaccga actggcaggt
    68101 gtggtacgag tgcgtgctgc cggaagatcg ccagcatgcc gaaaaagtga ttcgtgattc
    68161 gttgcaatca cgctcgccct ttaaactgga atttcgcatt accgtaaaag acggtattcg
    68221 ccatatccgc gccctcgcca accgggtact gaataaagaa ggcgaagtcg aacgtctcct
    68281 cggcatcaat atggatatga ccgaagtgaa acagcttaac gaggcattgt ttcaggaaaa
    68341 agagcgcctg cacattacgc ttgattccat cggtgaagcc gtggtctgta ttgatatggc
    68401 gatgaaaatt acctttatga atccagtggc ggagaagatg agcggctgga cgcaggaaga
    68461 agcgttaggt gttccgctcc tgacggtgtt gcatattact tttggcgaca acggaccatt
    68521 aatggagaac atttacagtg ccgacacctc acgttccgca atcgaacaag atgtggtgtt
    68581 gcactgtcgg agcggcggca gttacgacgt gcattacagt attacgccgt taagtactct
    68641 ggacggcagc aatattggtt cggttctggt gattcaggac gtcaccgaat cacgcaaaat
    68701 gctgcgccag ctgagctaca gcgcctccca tgatgcactg acgcatctcg ccaaccgcgc
    68761 cagttttgag aaacaactgc gtatcctgct gcaaacggta aacagtacgc atcagcgaca
    68821 tgccctggtg tttatcgatc ttgatcgctt taaagcggtg aatgacagcg ccgggcatgc
    68881 ggcgggcgac gctttgctgc gcgaactggc gtcgttgatg ctgagtatgc tgcgctccag
    68941 tgacgtgctg gcgcggctcg gcggtgatga atttggtctg ctgctgccag actgcaatgt
    69001 tgaaagcgct cgttttatcg ctacacgtat tatcagtgct gtgaatgact atcactttat
    69061 atgggaaggc cgggtacatc gggtaggtgc cagcgccggg attaccttga tcgatgacaa
    69121 caatcatcag gcggcagaag tgatgtcgca ggctgatatc gcctgttatg cctccaaaaa
    69181 tggtggacgg ggccgggtga cggtttacga accacagcaa gctgccgcac atagcgaacg
    69241 ggcggcgatg tcgcttgatg aacagtggcg gatgattaaa gagaatcagt tgatgatgat
    69301 cgcccacggt gtcgcttcgc cgcggatccc ggaagcgcgt aatttgtggc tgatttcgct
    69361 taagctctgg agttgcgaag gcgaaattat tgatgaacaa acatttcgtc gtagcttcag
    69421 cgatccggca cttagccatg ctcttgaccg acgggtattc cacgaatttt tccagcaggc
    69481 cgccaaagcg gttgccagta aaggcataag catcgccctc cccctttccg ttgccggttt
    69541 gagtagcgcc acgctggtga atgatctgct tgagcagctg gaaaatagcc ctctaccacc
    69601 acggttatta catctgatta ttccggctga agcgatttta gatcacgcag aaagcgtgca
    69661 aaaactgcag ctggcgggat gtcggatagt gctcagccag gtgggccgcg atctgcaaat
    69721 cttcaactcg ctgaaagcga atatggcaga ttacctgcta cttgatggtg agttatgcgc
    69781 caacgtgcag ggtaatttga tggatgagat gctgattacg attattcagg ggcacgctca
    69841 gcgactcggg atgaaaacca tcgccgggcc agtcgtttta cccttagtga tggatacgct
    69901 ttctggcatc ggcgtcgatc tgatttatgg tgatgtgatt gccgatgccc aaccgctgga
    69961 tttgctggtg aatagcagtt atttcgcgat taactgagag agcgtttgcc cattgcctga
    70021 tgcgacgcta acgcgtctta tcatgcctac aaatcgctca ttccccagac cggataaggc
    70081 gctcgcaccg catccggcga ccaacgtcat gcttcgtctg gttgccagcc ttccgtatac
    70141 cagatatgca acagcgcata agaacgccag ggcttccagc gctcggcata acggcggatt
    70201 tgtgccggtg tcattcccgg aaatcgccgt ttaatcagat aatcatccgg cagaaaaaca
    70261 tctttcgcct gccagccacg caaagcaaaa taattcgccg tccagcgccc gatacccgga
    70321 aaagtttgca gcgttttcat cgcctgctcc acatcgcccg gaattgtcat tggtaagctg
    70381 ccctccagcg ccgcatttgc cagatgaatc agtgcctctg cccgtttcaa cggcatgcct
    70441 aacgctttta atgcctgcgg gtcggctgct gccagccgct gaggcgtggg gaagcagata
    70501 tactccggaa aatcatccag ccgttcgcca taaagctgta ccactttggc agtcaatttt
    70561 gccgccatcg ccacgctcac cagttggcct aaaatcgccc gcacaccctg ctcaaaagca
    70621 tcaatacagc cgggtaaacg caatccgggc cgccccgcgc ctaactttcc caacgcaccg
    70681 ttaacaatct gcgggttaca ttgcagatca agcaggcggc tcattttcgc cagacactct
    70741 gcggcaacag gttctaaacc tgcacttaaa tttatgtgca gagtatggcg ggctatatcc
    70801 ggaatagcag tcactacgcc gcgatattcg cccaccgcca gactacgggc ataataattg
    70861 tccgcgaccg tttccacacc gctcaccgca cgggcggcga gaaatcccaa catccacgac
    70921 cagtcatacg gcggctgcca gttcagggta tacatcgcat ctccttgtta atccgctttc
    70981 agcataaacg ttattcagac gttacgcttt gctttcatat tccggttgtc gcgacagcaa
    71041 catttcgcta aagtcacgcc ccttcttcac cggcatgggg attaattcgt gtttattggt
    71101 tttgattacg gtacagcaaa ctgttcagtg gcggtcatgc gtgacggtaa accgcaatta
    71161 ctgaaaatgg aaaacgacag cacgctgctg ccttcaatgc tttgcgcgcc aacgcgtgaa
    71221 tcggtaagcg aatggctgta ccgccatcat gatgttccgg cagacgacga tgaaacgcag
    71281 gcgctgttac gtcgggcgat tcgttataac cgcgaagaag atatcgatgt tacggcgaaa
    71341 agcgtgcagt tcggtctttc ctcactggca cagtacattg atgatccaga agaagtgtgg
    71401 tttgtgaaat caccaaaatc gttcctcggt gccagcggct taaaaccgca gcaggtagcg
    71461 ctgtttgagg atctggtctg cgcaatgatg ttgcacattc gccagcaggc gcaggcgcag
    71521 ctgccagaag cgattactca ggcggtgatt ggtcgcccga tcaacttcca ggggctgggc
    71581 ggcgatgaag caaacgccca ggcgcaagag attctggaac gcgcggcgaa gcgtgccgga
    71641 ttcagggacg tggtattcca gtacgagccg atcgcggctg ggctggatta cgaagccacc
    71701 ttgcaggaag aaaaacgggt gctggtggtg gatatcggcg gtggtacgac tgactgttca
    71761 ttgctgctga tggggccgca gtggcgttcg cgtctcgatc gtgaagccag tctgctgggt
    71821 cacagtggtt gtcgtattgg cggtaacgat ctggatatcg ccctggcgtt taaaaacctg
    71881 atgccactgc tgggtatggg tggcgaaacc gaaaaaggca tcgccctgcc gatcctgccg
    71941 tggtggaatg cggttgccat caacgacgta cctgcgcaga gtgatttcta cagtagtgcc
    72001 aacggtcgtc tgcttaacga tctggtacgc gatgcccgcg aaccggaaaa agtggccctg
    72061 ttacagaaag tctggcgtca gcgtttaagc tatcgcctgg tgcgtagcgc agaagagtgc
    72121 aaaattgctc tttcaagcgt agcggaaacc cgcgcctcac tgccgtttat cagcaatgaa
    72181 ctggctacgc tgattagcca gcgagggctg gaaagcgccc tcagccagcc actggcgcgg
    72241 attctggaac aggtgcaact ggccctggat aacgcccagg aaaaaccgga cgttatctac
    72301 ctgaccggcg gtagcgcccg ttctccgcag attaaaaaag cgctggcaga acagttgccg
    72361 ggcattccga ttgcaggcgg cgatgacttt ggctccgtca ccgccgggct ggcacgctgg
    72421 gcggaagtgg tgtttcgtta attgaaaaat ggctcagcag cggcttaaat ctgcctgcgc
    72481 ctgagccagt ttttctgctg cctgacgtaa cggttccatc aacggcatgg tccgtgctgg
    72541 agcatgaagg cggaatcgct gcaactctgt cgcgccgact gtcaacgccg attccagccg
    72601 atgtcgtttg gcggtcattt tcgccattac cgcttgtctg tctgctggcg ttatcgcttc
    72661 attcggcctg aacacaaaac ggcgttcaca gttcgctttc cagtcgataa ccgcctgggt
    72721 cagatggtcg ccaaaccctt tcacttgctt aacgccacga cgggtaacat ctgcggctgt
    72781 ttcaatacca aaagaccgta acgccgcttt acgcgcaggg ccaacgccgg gaatagaggc
    72841 aacatcaata aaaaatccct ccagaaactt ctgcttctgc cgttcccttg cagtgtcatg
    72901 aagtgctgcc agagcgcgtt tctcttcttc cggtaacccg agaatttcgt ccttcatttt
    72961 ttcgagcatc gtccgtttgg cgataaaacc ttccaggccg cctacctgct ggatctggcg
    73021 gactaaatgg tcatagtcca ttttcgcacg attaaatgcc tcacggcgtt gctgaacttc
    73081 tgctttgtac gctttgcttg tcaaactgcc aataatccag atagccgcca gcacaggaac
    73141 caaaataata taacgcggtt ctgcctgaag gccgcaaagc agcagcgata atgctgacag
    73201 tgcgatctca agcagaatga tgtattcgcg ccgtaacagg cctaaaggaa gcggcctgcc
    73261 agtcggttgg aaatgatcgg gtaatggcag ttgcaatgct ggcggtgcta ctgacgccat
    73321 caccatcgcc cagacttttg ccagcacaaa atccccgccg gtggtaatga cctcttcgcc
    73381 gagatcaata aaatagataa caccttgatt atccagcgca caccatgggc agtcagccaa
    73441 atgaccggga taaacatgca ttgccgaaac ggtacatttc tttaactgtt ggcgtagaga
    73501 atccagtgct gatacccacg ctttagccgt cggacgcccg gttgccacgc cactttccgt
    73561 aaacgcctgc tgaaacatgg cttcaacatc actcggtaac atcgatagcg gaatcgatcg
    73621 tggcggcggt tttaaaccac gtcgctgatt atctgacgcg taggcataac ggaaatgggt
    73681 aatatccgtc tccagcgcat tacccgcatc agagataagc ggcacaccag aataaggatg
    73741 ccggccacca aacaagacgt gaaaaatcag caacgcaagg ccaaaattat cgtgattttc
    73801 tgtgcgttca aagccgacaa atgacgacaa ggtttgtagc tctggcggcg taaaatgcga
    73861 cacgccgact tcgcataaat gcagtgtgcc attggcgtta atctgaaagg agtcgctatc
    73921 aatcaacacc actttgctgt cgcgacctac cataaaactg ttctggttta cgtcacccac
    73981 gacgtgcccg tgctcgtgaa ccgtagcaaa agatgaagca atattgcgcg caacatagag
    74041 tagaaaatcc cacgcacaat gagggtaacg ttgacgacga tgtgccgggc tatagatcat
    74101 atgaatcggt tctttaccag aaacttttgg catcataaaa ccgataactt ttccgcctcg
    74161 tccaccgtga agcgttgcct gcggccaggc gacataattc aacaattgcg cgtcagctgt
    74221 cgcagccata aaggcaagtt tgtcctgttt taaggcgggt ggcggcgtgt gataaatctt
    74281 ggcgacgcta tcgacaaact cattgatatc ataaaccgcg ccttcaccgc ctttgcccag
    74341 ttcacggcct ggcgtaacgc actcaccagt agcagtatat aaagtgggtt tcatggtaac
    74401 cacagcgcca gtgccaacgt tttatcatca tccgtacgct cgtttaccgc cgggctggac
    74461 aaaaactgct ttaacaattc aggtaataaa tcgagttgtt cctgcgttgc tgacgccagt
    74521 ccattgaaaa acggggtaaa gaaaggcaca tgaggagaat tatccagcat atttaacgcc
    74581 agccgctgaa taccatccgt aaatgctgca actttatgcg cacgcccagt gctggtgaaa
    74641 gtttccagtc tggaaacggc atcttcgtcg gtaatgaagt gcgtcatgtt ggcatattca
    74701 cccaccatcg gcgtgagagg caattgcaga ccgtgaccca aatctaccac cacgccgcca
    74761 tcgccaatct gcataatcaa ggtgccgtca ggcgatgaaa tcaatcccag aaacgtgcag
    74821 gcaaaatcgc gaactgctaa tgctttggct tctgcctcgg caaaaagtcg ttgacgaata
    74881 gtcagtacca tatttgtggc gaggacatca ttaagcccca attcaccgtc ctgtactttt
    74941 tgcaccataa acgccatcgc ttcattgacg gcgagcgtcg cgccttctcc tccctgcgaa
    75001 acgctacctg cgccatcagc aaggaacatc accagtaatg gttgctggtc gtttaaccag
    75061 acaacctgca tctggcaggc atcctgacaa ggcaaatcag cactaatgtg cgatgttcca
    75121 acagcggatg cataaaccag acgccagctc acactgacgt ccagcctttt ggcgcttcca
    75181 gcacaacttc cgttcccggc gtggagcggg aaaccgaacg cagcgaactg gataaccagc
    75241 taaacagttc acggaattgt aatccctgca gtggcaaagg ctgacgaacg ctgatttgtg
    75301 ccagagtctt catatccgcc ccctgaacac cgatggaaaa gaaggcaaac ttcttatctt
    75361 cttccccctg aaacactttg ttggcggcag cctgccactc atcggttgga gcgccatcgg
    75421 tgatcaggaa aatccacggg cgataatacg aaataccatt tgcgcggtac tcacgtttcc
    75481 tctcttcaac catatccagg gctttggtaa tggcggcccc cataggcgta tccccctggg
    75541 caaacaggat gggcgggaaa aagttagcgg cgctggtaaa gggttgttcc acatgcaccg
    75601 ggccgaacgt cacaatgccg agttccactc tttttaaagc cagcggatca gcaagcagtt
    75661 catcacgaaa ggtaaccaat ccggcgttaa gttcattaat aggtctgccg ctcatcgagc
    75721 cagaaacatc cagcagcaaa atgcaggggc aacgtggttc cgggttactg gcaaaatcgc
    75781 tggtggcaaa cgtgatttgt tcgctcataa gaaatccttt cttagagaat tatcgaggtc
    75841 cggtatcata ccgattgcaa taatgaaata agagcaaacg aagaaacttt cagaattagg
    75901 cccgtcggcg cagaaaattt tgctattcgc ctcgcaagaa cggagagccg tcgattgaca
    75961 ctaagggcgg agtgacataa tttcaggagt gagggttagg gagaggtttc cccctccccc
    76021 tggtgttctt agtaagcctg gaagctaatc actaagagta tcaccagtat gatgacgtgc
    76081 ttcatcataa ccctttcctt attaaaagcc ctcttctccg ggagagggtt tcccgtttca
    76141 gcgtcccgct gaaatcgtcg gcttacctcc tttcgccctg aatgcagtct atcgctaacg
    76201 cgtgaacaaa atagtttcat ctgtgattat tgcgtaacga ctcgcaaaga atatttgctg
    76261 gcaggatcgc agactacaaa gcctgcggat tgacaatctt atcgtgaagg cataatttca
    76321 ggagtgaggg tagagcgggg tttcccccgc cctggtagtc ttagtaagcg gggaagctta
    76381 tgactaagag caccacgatg atgagtagct tcatcatgac cctttcctta ttaaggcccc
    76441 ttcctcggga ggggctttcc cgtttcagcg tcccgctgaa atcgtcggct tacctccttt
    76501 cgccctgtat gcagtctatc gctgacttgc ggataaaata gtttccagtg tgattattgc
    76561 gtaacgtctc gcaaagaata tttgctggca ggatcgcaga ctgcaaagcc tgcagattga
    76621 caatcttatc gtgaaggcat actttcagga gtgagggtag agcggggttt cccccgccct
    76681 ggtagcctta gtaagctggc cagctaatga ctaagagcac cacgatgatg agtagcttca
    76741 tcatgaccct ttccttattt atggcccctt cctcgggagg ggctttcccg tttcagcgtc
    76801 ccgctgaaat cggcggctta cctcctttcg ccatgcaagc agtctatcgc taacgcgtag
    76861 ataaaatagt ttcctgtgtt attactggat gcgtgctcgc aaatgtgccc gtcattcaga
    76921 cgattcccga cagtgtttca taattcctcc atttttctcc cttattggct ggctacacta
    76981 gtatcattcc gcgaaacgtt tcaggaagag aaactcttaa cgatgaaagg cagttataaa
    77041 tcccgttggg taatcgtggt ggttatcgcc gccatcgccg cattctggtt ctggcaaggc
    77101 cgcaatgact cccagagtgc agccccaggg gcgacgaaac aagcgcagca atcgccagcg
    77161 ggtggtcgcc gtggtatgcg ttccggccca ttagccccgg ttcaggcggc gaccgccgta
    77221 gaacaggcag ttccgcgtta cctcaccggg cttggcacca ttatcgccgc taatactgtt
    77281 acggtgcgca gccgcgtgga cggtcaactg atggcgttac atttccagga aggccagcag
    77341 gtcaaagcag gcgatttact ggcagaaatt gaccccagcc agttcaaagt tgcattagca
    77401 caaacccagg gccaactggc aaaagataaa gccacgcttg ctaacgcccg ccgtgatctg
    77461 gcgcgttatc agcaactggc aaaaaccaat ctcgtctccc gtcaggagct ggatgcccaa
    77521 caggcgctgg tcagtgaaac cgaaggcacc attaaggctg atgaagcaag cgtcgccagc
    77581 gcgcagttgc aactcgactg gagccgcatc accgcaccag tcgatggtcg cgttggtctc
    77641 aagcaggttg atgttggtaa ccaaatctcc agtggtgata ccaccggaat tgtggtgatc
    77701 acccagacgc atcctatcga tttagtcttt accctgccgg aaagcgatat cgctactgta
    77761 gtgcaggcgc aaaaagccgg aaaaacgctg gtggtagaag cctgggatcg caccaattcg
    77821 aagaaattaa gtgaaggcac gctgttaagc ctcgataacc aaatcgatgc cacaaccggt
    77881 acgattaaag tgaaagcacg ctttaataat caggatgatg cgctgtttcc caatcagttt
    77941 gttaacgcgc gcatgttagt cgacaccgaa caaaacgccg tagtgatccc aacagccgcc
    78001 ctgcaaatgg gcaatgaagg ccattttgtc tgggtgctga atagcgaaaa caaggtcagc
    78061 aaacatctgg tgacgccggg cattcaggac agtcagaaag tggtgatccg tgcaggtatt
    78121 tctgcgggcg atcgcgtggt gacagacggc attgatcgcc tgaccgaagg ggcgaaagtg
    78181 gaagtggtgg aagcccagag cgccaccact ccggaagaga aagccaccag ccgcgaatac
    78241 gcgaaaaaag gagcacgctc ctgatgcagg tgttaccccc gagcagcaca ggcggcccgt
    78301 cgcgcctgtt tattatgcgt cctgtcgcca ccacgctgct gatggtggcg atcttactcg
    78361 ccgggattat cggttatcgc gccctgcccg tttcggcgct gccggaagtg gactatccga
    78421 ccattcaggt ggtcacgctc tacccaggtg ccagcccgga tgtcatgacc tctgccgtta
    78481 ccgcgccgct cgaacgccag ttcgggcaga tgtctggcct gaaacagatg tcgtcgcaaa
    78541 gttccggcgg tgcgtcagtt atcactttgc agttccagct aacattaccg ctcgatgtcg
    78601 ccgagcagga agtgcaggcc gcgattaacg ctgcgaccaa cttgttgccg agcgatctgc
    78661 ctaacccgcc ggtttacagc aaagtgaacc cggcagatcc gccgatcatg acgctcgccg
    78721 tcacctcaac cgccatgccg atgacgcaag tggaagatat ggtggaaacc cgcgtcgcgc
    78781 agaaaatctc gcagatttcc ggcgtcggcc tggtgacgct ttccggcggt cagcgtccgg
    78841 ctgttcgcgt caaacttaac gctcaggcga ttgccgccct cggcctgacc agcgaaaccg
    78901 tgcgcaccgc cattaccggc gctaacgtta actcggcaaa aggtagcctc gacggccctt
    78961 cccgtgcggt cacgctttcc gcgaacgacc agatgcaatc cgccgaagag tatcgccagc
    79021 taatcatcgc ctaccagaac ggcgcgccaa ttcgtctggg cgatgtcgca actgtagagc
    79081 aaggtgcaga aaacagctgg ctcggcgcgt gggcgaacaa agaacaggcc attgtgatga
    79141 atgttcagcg ccagcccggt gctaacatta tctccaccgc cgacagcatt cggcagatgc
    79201 tgccacagct cactgagagt ctgccgaaat cggtgaaggt gacagtgctt tccgatcgca
    79261 ccaccaatat ccgcgcatcc gtcgatgata ctcagtttga attgatgatg gctatcgcgc
    79321 tggtagtcat gattatctac ctgtttttgc gcaatattcc ggcgaccatc attcccggtg
    79381 ttgctgtacc gctgtcgtta atcggcactt tcgcggttat ggtgtttctc gatttttcaa
    79441 tcaataacct gacactgatg gcgttaacta tcgccaccgg attcgtggtc gatgacgcca
    79501 tcgtggtgat cgaaaacatt tcccgctata tcgaaaaagg cgaaaaaccg ttggcggcgg
    79561 cgctcaaggg cgcaggtgaa atcggcttta ccattatctc gctgaccttc tcactgattg
    79621 cggtgttgat cccactgctg tttatgggcg atatcgtcgg gcgactgttc cgcgaatttg
    79681 ctattaccct ggcggtagcg attttgatct cagcggtggt gtcgctgacc ctgacaccga
    79741 tgatgtgcgc gcggatgctc agccaggagt cgttgcgtaa acagaaccgc ttctcccgtg
    79801 cctcggaaaa aatgttcgac aggataatcg ccgcctatgg tcgtggactg gcgaaagtgc
    79861 tgaatcatcc gtggctgacc ttaagcgtgg cactcagcac gctgctgctt agcgtgctgc
    79921 tgtgggtgtt cattccgaaa ggtttcttcc cggtacagga caatggcatt attcagggca
    79981 ctttgcaggc accgcaatcc agctccttta ccaatatggc ccagcgacaa cgccaggtcg
    80041 cggacgtgat tttgcaggat ccggcagtgc aaagcctgac ctcatttgtt ggcgttgatg
    80101 gcactaaccc gtcgctgaat agtgcacgtt tacaaatcaa cctcaaaccg ttggatgaac
    80161 gtgatgatcg ggtgcaaaaa gtcatcgccc gtctgcaaac ggcggtagat aaagtgccgg
    80221 gcgtcgatct cttcctgcaa ccaacgcagg atctgactat tgatactcag gtcagccgca
    80281 cccagtacca gtttaccttg caggccacct cactggatgc gctcagtacc tgggtgccac
    80341 agttgatgga aaaactccag caactgccac agctttctga tgtctccagc gactggcagg
    80401 accaagggct ggtggcgtat gtcaatgttg atcgcgacag cgccagccgt ctggggatca
    80461 gcatggcgga tgtcgataac gccctgtaca acgcgtttgg tcagcgactg atttccacta
    80521 tttatactca ggctaaccag tatcgcgtag tgctggagca caacaccgaa attaccccgg
    80581 gcctcgcggc gctggatacc attcgcctga ccagtagcga cggcggcgtg gtgccgttaa
    80641 gctcaattgc caaagttgaa cagcgttttg cgccgctttc catcaaccat ctggatcagt
    80701 tcccggtaac gaccatctcc tttaacgtgc cggataacta ttcgctgggc gatgcggtgc
    80761 aggagattat ggacaccgaa aagacgctga atctgccggt ggatatcacc acgcaattcc
    80821 agggcagcac cctcgccttc cagtcggcgc tgggcagcac tgtctggctg attgtcgcgg
    80881 cggtggtggc gatgtatatc gtgctcggca ttctgtacga gagctttatt cacccgatca
    80941 ccattctctc gacgctaccc accgcagggg tcggcgcgct gctggcatta atgattgccg
    81001 gtagcgaact ggatgtgatt gcgattatcg gcattatttt gctgatcggt attgtgaaga
    81061 agaacgccat catgatgatc gacttcgcgc tggctgctga gcgcgagcaa ggcatgtcgc
    81121 cgcgcgatgc aatctaccag gcttgtctgt tgcgttttcg tccgatcctg atgaccaccc
    81181 tggcggctct gcttggcgcg ctgccgctga tgttgagtac cggggtcggc gcggaactgc
    81241 gtcgtccgtt aggtatcggc atggtcggcg gtctgattgt cagccaggtg ctgacgctgt
    81301 tcaccacgcc ggtgatttat ctgctgttcg accgcctggc attgtggacc aaaagtcgct
    81361 ttgctcgcca tgaagaggag gcgtaagtga agttttttgc cctcttcatt taccgcccgg
    81421 tggcgacgat tttactgtcg gtcgccatta ccctgtgcgg catactgggc ttccgtatgc
    81481 tgccggtcgc cccgctgccg caggtcgatt ttccggtgat tatggtcagc gcctcgctgc
    81541 ccggtgcgtc accagaaaca atggcgtctt ccgttgccac gccgctggag cgctcgcttg
    81601 ggcgcattgc cggggtcagc gaaatgacct ccagcagttc gctcggcagc acacgtatta
    81661 ttttacagtt tgattttgac cgggacatca acggcgcagc gcgtgatgtg caggcggcga
    81721 tcaacgctgc acaaagtttg ctgcccagtg ggatgcccag ccgtccgacc tatcgcaaag
    81781 cgaacccgtc ggatgcgcca attatgatcc tcacgctgac atccgatact tattcgcagg
    81841 gtgaactgta cgatttcgcc tcgacgcagc tggctccgac gatttcgcaa atcgacggtg
    81901 ttggtgatgt cgatgtcgga ggcagctcac tgcccgccgt acgcgtcggg ctgaatccgc
    81961 aggcgctgtt taatcagggc gtgtcgctgg acgacgtacg caccgccatc agcaatgcca
    82021 acgtgcgtaa accgcagggc gcgctggaag atggcactca ccgctggcag atccagacca
    82081 atgatgagct aaaaaccgcc gctgaatatc agccgttgat tattcactac aacaacggcg
    82141 gcgcggttcg tctgggcgat gtggcgacgg tgaccgactc agtgcaggat gtgcgcaacg
    82201 ccgggatgac caacgccaaa ccggctattt tactgatgat ccgcaaactg ccggaagcca
    82261 atattatcca gacggttgac agcatccggg caaaattacc ggagttgcag gaaaccattc
    82321 cggcggcgat tgatctgcaa attgcccagg atcgctcccc caccattcgc gcctcgctgg
    82381 aagaagtcga gcaaacgctg attatctcgg tggcgctggt gattctggtg gtgtttttat
    82441 tcctgcgctc gggtcgcgcc actattattc ccgccgtttc ggtgccggtt tcgctgattg
    82501 gtacgtttgc ggcgatgtac ctgtgcggat tcagtctcaa taacctttcg ttaatggcgc
    82561 tcaccatcgc tactggtttc gtggtggatg acgccatcgt ggtgctggaa aacattgcac
    82621 gtcatctgga agcgggaatg aaaccgttgc aagccgcact gcaaggtact cgcgaagtcg
    82681 gttttacggt gctgtcgatg agtctgtcac tggtggcggt gttcctgccg ctgctgttga
    82741 tgggcggatt gccgggccga ctgttacgcg agtttgccgt gacgctttct gtcgccattg
    82801 gtatttcgtt gctggtttct ctgacattaa cgccaatgat gtgtggctgg atgctgaaag
    82861 ccagcaagcc gcgcgagcaa aagcgactgc gtggttttgg tcgcatgttg gtagccctgc
    82921 aacaaggcta cggcaagtca ctgaaatggg tgctcaatca tacccgtctg gtgggcgtgg
    82981 tgctgcttgg caccattgcg ctgaatatct ggctgtatat ctcgatcccg aaaaccttct
    83041 tcccggagca ggacactggc gtgttgatgg gcgggattca ggcggatcag agtatttcgt
    83101 ttcaggcgat gcgcggtaag ttgcaggatt tcatgaaaat tatccgtgac gatccggcag
    83161 tggataatgt caccggcttt acaggcggtt cgcgagtgaa cagcgggatg atgtttatca
    83221 ccctcaagcc acgcgacgaa cgcagcgaaa cggcgcagca aattatcgac cgtctgcgcg
    83281 taaaactggc gaaagaaccg ggggcgaatc tgttcctgat ggcggtacag gatattcgcg
    83341 ttggtgggcg tcagtcgaac gccagctacc agtacacgtt gttatccgac gacctggcgg
    83401 cactgcgcga atgggagccg aaaatccgca aaaaactggc gacgttgccg gaactggcgg
    83461 acgtgaactc cgatcagcag gataacggcg cggagatgaa tctggtttac gaccgcgaca
    83521 ccatggcacg gctgggaatc gacgtgcagg ccgccaacag cctgttaaat aacgccttcg
    83581 gtcagcggca aatctcgacc atttaccagc cgatgaacca gtataaagtg gtgatggaag
    83641 tggatccgcg ctatacccag gacatcagtg cgctggaaaa aatgttcgtt atcaataacg
    83701 aaggcaaagc gatcccgctg tcgtatttcg ctaaatggca accggcgaac gctccgctgt
    83761 cggtgaatca tcagggatta tcggcggcct cgaccatctc gttcaacctg ccgaccggaa
    83821 aatcgctctc ggacgccagt gcggcgatcg atcgcgcaat gacccagctt ggtgtgcctt
    83881 cgacggtgcg cggcagtttt gccggaacag cgcaggtgtt ccaggagacg atgaactcac
    83941 aggtgatcct gattatcgcc gccatcgcca cggtgtatat cgtgctgggt atcctttacg
    84001 agagttacgt acacccactg acgattctct ccaccctgcc ctcggcgggc gttggtgcgc
    84061 tgctggcgct ggagctgttc aatgccccgt tcagcctaat agccctgata gggatcatgc
    84121 tattaatcgg catcgtaaag aaaaacgcca ttatgatggt cgattttgcg ctcgaagccc
    84181 aacggcacgg taacctgacg ccgcaggaag ctattttcca ggcctgtctg ctgcgttttc
    84241 gcccgattat gatgactacc ctggcggcgc tgtttggcgc gctgccgctg gtgttgtcgg
    84301 gcggtgacgg ctcggagctg cggcacctcc tggggatcac cattgtcggt ggactggtaa
    84361 tgagccagct tctgacgctg tataccacgc cggtggtgta tctctttttc gaccgtctgc
    84421 ggctgcgttt ttcgcgtaaa cctaaacaaa cggtaaccga gtaaatgaca gaacttcccg
    84481 acagcacccg ctggcaattg tggattgtgg ctttcggctt ctttatgcag tcgctggaca
    84541 ccaccatcgt taacaccgcc cttccctcaa tggcgcaaag cctcggggaa agtccgttgc
    84601 atatgcacat ggtcattgtc tcttatgtgc tgaccgtggc ggtgatgctg cccgccagcg
    84661 gctggctggc ggacaaagtc ggcgtgcgca atattttctt taccgccatc gtgctgttta
    84721 ctctcggttc actgttttgc gcgctttccg gcacgctgaa cgaactgttg ctggcacgcg
    84781 cgttacaggg cgttggcggt gcgatgatgg tgccggtcgg caggttgacg gtgatgaaaa
    84841 tcgtaccgcg cgagcaatat atggcggcga tgacctttgt cacgttaccc ggtcaggtcg
    84901 gtccgctgct cggtccggcg ctcggcggtc tgctggtgga gtacgcgtcg tggcactgga
    84961 tctttttgat caacattccg gtggggatta tcggtgcgat cgccacattg ctgttaatgc
    85021 cgaactacac catgcagacg cggcgctttg atctttccgg ctttttattg ctggcggttg
    85081 gcatggctgt attaaccctg gcgctggacg gcagtaaagg tacaggttta tcgccgctgg
    85141 cgattgctgg cctggtcgca gttggcgtgg tggcactggt gctttatctg ctgcacgcca
    85201 gaaataacaa ccgcgccctg ttcagtctga aactgttccg cactcgtacc ttttcgctgg
    85261 gcctggcggg gagctttgcc ggacgtattg gcagtggcat gttgcccttt atgacaccgg
    85321 ttttcctgca aattggcctc ggtttctcgc cgtttcatgc cggactgatg atgatcccga
    85381 tggtacttgg cagcatggga atgaagcgaa ttgtggttca ggtggtgaat cgctttggtt
    85441 atcgtcgggt actggtagcg accacgctgg gtctgtcgct gatcaccctg ttgtttatga
    85501 ctaccgctct gttgggctgg tactacgttt tgccgttcgt cctgttttta caagggatgg
    85561 tcaactcgac ccgtttctcc tccatgaaca ccctgacgct gaaagatctc ccggacaatc
    85621 tggcgagcag cggcaacagt ctgctgtcga tgattatgca attgtcgatg agtatcggcg
    85681 tcaccatcgc cgggctgttg ctgggacttt ttggttcgca gcatgtcagc gtcgacagcg
    85741 gcaccacaca aaccgtcttt atgtacacct ggcttagcat ggcgtttatc atcgcccttc
    85801 cggcgttcat ctttgccaga gtgccgaacg atacgcatca aaatgtagct atttcgcggc
    85861 gaaaaaggag cgcgcaatga agttctggcg acctggtatt accggcaaac tgtttctggc
    85921 gattttcgcc acctgcattg ttttgctgat cagtatgcac tgggcggtgc gtatcagttt
    85981 tgagcgtggc tttattgatt acatcaagca tggtaatgaa cagcgattac aactgttaag
    86041 tgatgcgctt ggcgagcagt atgcgcagca tggcaactgg cgcttcctgc gcaacaatga
    86101 tcgctttgtc tttcagatcc tgcgttcatt tgaacacgat aattcggaag ataaacccgg
    86161 cccgggtatg ccaccgcacg gctggcgtac ccagttctgg gtggttgatc aaaacaacaa
    86221 agtgctggtt ggtccgcgag cgccgattcc acctgacggt acacggcgac ccattctggt
    86281 caacggtgcg gaagttggcg cggtgatcgc ctcccccgtt gagcggttaa cgcgcaatac
    86341 tgatatcaat ttcgataaac aacagcggca aaccagctgg ttgattgtcg ccctggcaac
    86401 gttactcgcg gcacttgcca cttttctgct ggcgcgcggt ttactggcac cggtaaaacg
    86461 acttgtcgat ggcacgcaca aactggcggc gggcgatttc actacccgcg taacgcccac
    86521 cagtgaagat gaactgggca aactggcgca agacttcaac cagcttgcca gcacactgga
    86581 gaaaaaccag caaatgcggc gcgattttat ggccgatatt tctcacgaac tgcgtacgcc
    86641 attagcggtg ctgcgcggtg aactggaagc tattcaggat ggcgtgcgca aattcacgcc
    86701 ggagacggtg gcgtctttac aggcggaggt cggtacactg accaaactgg ttgacgatct
    86761 ccatcagttg tcgatgtctg atgaaggcgc cctcgcctat caaaaagcac cggtagattt
    86821 gatcccactg ctggaagtgg cgggcggcgc atttcgcgaa cgattcgcca gccgtggcct
    86881 gaaactgcaa ttttccctgc cagacagtat taccgtattt ggcgatcgcg accgtttaat
    86941 gcagttattc aataatttac ttgaaaacag cctgcgctac actgacagcg gcggcagcct
    87001 gaaaatctct gccgagcagc acgacaaaac ggtgcgcctg acctttgccg acagtgcgcc
    87061 aggtgtcagt gacgagcagc tacaaaaatt atttgaacgt ttttatcgca ccgaaggttc
    87121 ccgcaaccgc gccagcggtg gttccgggct ggggctggcg atttgcctga acattgttga
    87181 agcacataat ggccgcatta ttgctgccca ttcgcctttt ggcggggtaa gcattacagt
    87241 agagttaccg ctggaacggg atttacagag agaagtatga ccgagttacc aatcgacgaa
    87301 aacacaccgc gtattttgat cgtggaagat gaaccgaagc tggggcagtt gctcattgat
    87361 tatctgcgtg ctgcgagcta tgcgccgacg cttatcagcc acggcgatca ggtactgccg
    87421 tatgtgcgcc agacaccacc ggatctgatc ctgttggatc tgatgctccc tggcaccgat
    87481 ggcttgacgc tgtgccggga aattcgtcgt ttttctgaca ttcctatcgt gatggtgacg
    87541 gcaaaaatcg aagagatcga ccgcctgctc ggactggaga ttggcgcaga tgactacatc
    87601 tgcaagccgt acagcccacg ggaagtggta gcgcgcgtca aaaccatttt gcgccgttgc
    87661 aaaccgcagc gcgaattgca gcaacaggat gccgaaagcc cgttgattat cgacgaaggt
    87721 cgttttcagg cttcatggcg cggtaaaatg cttgacctga cgcctgcgga atttcgtctg
    87781 ttgaaaacgc tctctcatga accaggaaaa gtgttctccc gcgagcaatt gctcaatcat
    87841 ctttatgacg actaccgcgt agtaaccgac cgcaccattg acagccacat taaaaacctg
    87901 cgccgcaagc tggaatctct cgacgccgaa cagtcattta tccgcgccgt ttacggcgtt
    87961 ggttaccgct gggaagccga cgcctgccgc atcgtttagt tttagcgaca tgactttgtt
    88021 agccggagat ggcgttcaga ccagatccgg caacattatc ccacgcatgg tcagcaaact
    88081 g
//
LOCUS       DAETQN010000003        79578 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.003, whole genome shotgun sequence.
ACCESSION   DAETQN010000003 DAETQN010000000
VERSION     DAETQN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 79578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.003"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="K0W68_000213"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="K0W68_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /gene="bluR"
                     /locus_tag="K0W68_000214"
     CDS             1402..2133
                     /gene="bluR"
                     /locus_tag="K0W68_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HBI3071898.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..2758
                     /locus_tag="K0W68_000215"
     CDS             2354..2758
                     /locus_tag="K0W68_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HBI3071899.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2811..2921
                     /locus_tag="K0W68_000216"
     CDS             2811..2921
                     /locus_tag="K0W68_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071900.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            3157..3201
                     /locus_tag="K0W68_000217"
     CDS             3157..3201
                     /locus_tag="K0W68_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3071901.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            3454..3777
                     /gene="iraM"
                     /locus_tag="K0W68_000218"
     CDS             3454..3777
                     /gene="iraM"
                     /locus_tag="K0W68_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBI3071902.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(3880..5130)
                     /gene="icd"
                     /locus_tag="K0W68_000219"
     CDS             complement(3880..5130)
                     /gene="icd"
                     /locus_tag="K0W68_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBI3071903.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            5302..5955
                     /gene="rluE"
                     /locus_tag="K0W68_000220"
     CDS             5302..5955
                     /gene="rluE"
                     /locus_tag="K0W68_000220"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBI3071904.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            5965..6426
                     /gene="nudJ"
                     /locus_tag="K0W68_000221"
     CDS             5965..6426
                     /gene="nudJ"
                     /locus_tag="K0W68_000221"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBI3071905.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            6480..7586
                     /gene="mnmA"
                     /locus_tag="K0W68_000222"
     CDS             6480..7586
                     /gene="mnmA"
                     /locus_tag="K0W68_000222"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBI3071906.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            7622..8263
                     /gene="hflD"
                     /locus_tag="K0W68_000223"
     CDS             7622..8263
                     /gene="hflD"
                     /locus_tag="K0W68_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBI3071907.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            8267..9637
                     /gene="purB"
                     /locus_tag="K0W68_000224"
     CDS             8267..9637
                     /gene="purB"
                     /locus_tag="K0W68_000224"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBI3071908.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            9805..10476
                     /gene="phoP"
                     /locus_tag="K0W68_000225"
     CDS             9805..10476
                     /gene="phoP"
                     /locus_tag="K0W68_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBI3071909.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            10476..11936
                     /gene="phoQ"
                     /locus_tag="K0W68_000226"
     CDS             10476..11936
                     /gene="phoQ"
                     /locus_tag="K0W68_000226"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBI3071910.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            12012..13133
                     /gene="roxA"
                     /locus_tag="K0W68_000227"
                     /gene_synonym="ycfD"
     CDS             12012..13133
                     /gene="roxA"
                     /locus_tag="K0W68_000227"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBI3071911.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="K0W68_000228"
     CDS             complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="K0W68_000228"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBI3071912.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            14658..15794
                     /gene="potA"
                     /locus_tag="K0W68_000229"
     CDS             14658..15794
                     /gene="potA"
                     /locus_tag="K0W68_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBI3071913.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15778..16635
                     /gene="potB"
                     /locus_tag="K0W68_000230"
     CDS             15778..16635
                     /gene="potB"
                     /locus_tag="K0W68_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBI3071914.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            16632..17426
                     /gene="potC"
                     /locus_tag="K0W68_000231"
     CDS             16632..17426
                     /gene="potC"
                     /locus_tag="K0W68_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBI3071915.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            17423..18469
                     /gene="potD"
                     /locus_tag="K0W68_000232"
     CDS             17423..18469
                     /gene="potD"
                     /locus_tag="K0W68_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBI3071916.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="K0W68_000233"
     CDS             complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="K0W68_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBI3071917.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="K0W68_000234"
     CDS             complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="K0W68_000234"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBI3071918.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="K0W68_000235"
     CDS             complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="K0W68_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBI3071919.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="K0W68_000236"
     CDS             complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="K0W68_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBI3071920.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="K0W68_000237"
     CDS             complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="K0W68_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBI3071921.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            23801..24874
                     /locus_tag="K0W68_000238"
     CDS             23801..24874
                     /locus_tag="K0W68_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBI3071922.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            25002..28448
                     /gene="mfd"
                     /locus_tag="K0W68_000239"
     CDS             25002..28448
                     /gene="mfd"
                     /locus_tag="K0W68_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBI3071923.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            28592..29548
                     /gene="ldtC"
                     /locus_tag="K0W68_000240"
                     /pseudo
     CDS             28592..29548
                     /gene="ldtC"
                     /locus_tag="K0W68_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="K0W68_000241"
     CDS             complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="K0W68_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HBI3071924.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            30129..30761
                     /gene="comR"
                     /locus_tag="K0W68_000242"
     CDS             30129..30761
                     /gene="comR"
                     /locus_tag="K0W68_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBI3071925.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(30823..31362)
                     /locus_tag="K0W68_000243"
     CDS             complement(30823..31362)
                     /locus_tag="K0W68_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBI3071926.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="K0W68_000244"
     CDS             complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="K0W68_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBI3071927.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(33301..33843)
                     /locus_tag="K0W68_000245"
     CDS             complement(33301..33843)
                     /locus_tag="K0W68_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI3071928.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(33866..34891)
                     /gene="nagZ"
                     /locus_tag="K0W68_000246"
     CDS             complement(33866..34891)
                     /gene="nagZ"
                     /locus_tag="K0W68_000246"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBI3071929.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(34902..35726)
                     /gene="thiK"
                     /locus_tag="K0W68_000247"
     CDS             complement(34902..35726)
                     /gene="thiK"
                     /locus_tag="K0W68_000247"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBI3071930.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(35707..36348)
                     /gene="lpoB"
                     /locus_tag="K0W68_000248"
     CDS             complement(35707..36348)
                     /gene="lpoB"
                     /locus_tag="K0W68_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBI3071931.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVKAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(36362..36739)
                     /locus_tag="K0W68_000249"
     CDS             complement(36362..36739)
                     /locus_tag="K0W68_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBI3071932.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(36742..37101)
                     /gene="hinT"
                     /locus_tag="K0W68_000250"
     CDS             complement(36742..37101)
                     /gene="hinT"
                     /locus_tag="K0W68_000250"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBI3071933.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            37435..39624
                     /gene="fhuE"
                     /locus_tag="K0W68_000251"
     CDS             37435..39624
                     /gene="fhuE"
                     /locus_tag="K0W68_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HBI3071934.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(39684..41117)
                     /gene="ptsG"
                     /locus_tag="K0W68_000252"
     CDS             complement(39684..41117)
                     /gene="ptsG"
                     /locus_tag="K0W68_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBI3071935.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(41412..42209)
                     /locus_tag="K0W68_000253"
     CDS             complement(41412..42209)
                     /locus_tag="K0W68_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBI3071936.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(42220..43224)
                     /gene="holB"
                     /locus_tag="K0W68_000254"
     CDS             complement(42220..43224)
                     /gene="holB"
                     /locus_tag="K0W68_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBI3071937.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(43221..43862)
                     /locus_tag="K0W68_000255"
     CDS             complement(43221..43862)
                     /locus_tag="K0W68_000255"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBI3071938.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(43852..44874)
                     /gene="yceG"
                     /locus_tag="K0W68_000256"
     CDS             complement(43852..44874)
                     /gene="yceG"
                     /locus_tag="K0W68_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBI3071939.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(44877..45686)
                     /gene="pabC"
                     /locus_tag="K0W68_000257"
     CDS             complement(44877..45686)
                     /gene="pabC"
                     /locus_tag="K0W68_000257"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBI3071940.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(45806..47047)
                     /gene="fabF"
                     /locus_tag="K0W68_000258"
     CDS             complement(45806..47047)
                     /gene="fabF"
                     /locus_tag="K0W68_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBI3071941.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(47135..47371)
                     /gene="acpP"
                     /locus_tag="K0W68_000259"
     CDS             complement(47135..47371)
                     /gene="acpP"
                     /locus_tag="K0W68_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBI3071942.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(47582..48316)
                     /gene="fabG"
                     /locus_tag="K0W68_000260"
     CDS             complement(47582..48316)
                     /gene="fabG"
                     /locus_tag="K0W68_000260"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBI3071943.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(48329..49258)
                     /gene="fabD"
                     /locus_tag="K0W68_000261"
     CDS             complement(48329..49258)
                     /gene="fabD"
                     /locus_tag="K0W68_000261"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBI3071944.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(49274..50227)
                     /gene="fabH"
                     /locus_tag="K0W68_000262"
     CDS             complement(49274..50227)
                     /gene="fabH"
                     /locus_tag="K0W68_000262"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBI3071945.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(50295..51365)
                     /gene="plsX"
                     /locus_tag="K0W68_000263"
     CDS             complement(50295..51365)
                     /gene="plsX"
                     /locus_tag="K0W68_000263"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBI3071946.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(51446..51619)
                     /gene="rpmF"
                     /locus_tag="K0W68_000264"
     CDS             complement(51446..51619)
                     /gene="rpmF"
                     /locus_tag="K0W68_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBI3071947.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(51671..52192)
                     /gene="yceD"
                     /locus_tag="K0W68_000265"
     CDS             complement(51671..52192)
                     /gene="yceD"
                     /locus_tag="K0W68_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBI3071948.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            52391..52975
                     /gene="yceF"
                     /locus_tag="K0W68_000266"
     CDS             52391..52975
                     /gene="yceF"
                     /locus_tag="K0W68_000266"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBI3071949.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(53087..54046)
                     /gene="rluC"
                     /locus_tag="K0W68_000267"
     CDS             complement(53087..54046)
                     /gene="rluC"
                     /locus_tag="K0W68_000267"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBI3071950.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            54619..57804
                     /gene="rne"
                     /locus_tag="K0W68_000268"
     CDS             54619..57804
                     /gene="rne"
                     /locus_tag="K0W68_000268"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBI3071951.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(57999..58952)
                     /gene="flgL"
                     /locus_tag="K0W68_000269"
     CDS             complement(57999..58952)
                     /gene="flgL"
                     /locus_tag="K0W68_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBI3071952.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(58964..60607)
                     /gene="flgK"
                     /locus_tag="K0W68_000270"
     CDS             complement(58964..60607)
                     /gene="flgK"
                     /locus_tag="K0W68_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBI3071953.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(60673..61614)
                     /gene="flgJ"
                     /locus_tag="K0W68_000271"
     CDS             complement(60673..61614)
                     /gene="flgJ"
                     /locus_tag="K0W68_000271"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBI3071954.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(61614..62711)
                     /gene="flgI"
                     /locus_tag="K0W68_000272"
     CDS             complement(61614..62711)
                     /gene="flgI"
                     /locus_tag="K0W68_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBI3071955.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(62723..63421)
                     /gene="flgH"
                     /locus_tag="K0W68_000273"
     CDS             complement(62723..63421)
                     /gene="flgH"
                     /locus_tag="K0W68_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBI3071956.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(63474..64246)
                     /gene="flgG"
                     /locus_tag="K0W68_000274"
                     /pseudo
     CDS             complement(63474..64246)
                     /gene="flgG"
                     /locus_tag="K0W68_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(64418..65173)
                     /gene="flgF"
                     /locus_tag="K0W68_000275"
     CDS             complement(64418..65173)
                     /gene="flgF"
                     /locus_tag="K0W68_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HBI3071957.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(65193..66398)
                     /gene="flgE"
                     /locus_tag="K0W68_000276"
     CDS             complement(65193..66398)
                     /gene="flgE"
                     /locus_tag="K0W68_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBI3071958.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(66423..67118)
                     /gene="flgD"
                     /locus_tag="K0W68_000277"
     CDS             complement(66423..67118)
                     /gene="flgD"
                     /locus_tag="K0W68_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBI3071959.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(67130..67534)
                     /gene="flgC"
                     /locus_tag="K0W68_000278"
     CDS             complement(67130..67534)
                     /gene="flgC"
                     /locus_tag="K0W68_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBI3071960.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(67538..67954)
                     /gene="flgB"
                     /locus_tag="K0W68_000279"
     CDS             complement(67538..67954)
                     /gene="flgB"
                     /locus_tag="K0W68_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBI3071961.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            68109..68768
                     /gene="flgA"
                     /locus_tag="K0W68_000280"
     CDS             68109..68768
                     /gene="flgA"
                     /locus_tag="K0W68_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBI3071962.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            68844..69137
                     /gene="flgM"
                     /locus_tag="K0W68_000281"
     CDS             68844..69137
                     /gene="flgM"
                     /locus_tag="K0W68_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBI3071963.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            69142..69558
                     /gene="flgN"
                     /locus_tag="K0W68_000282"
     CDS             69142..69558
                     /gene="flgN"
                     /locus_tag="K0W68_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBI3071964.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(69598..71133)
                     /gene="murJ"
                     /locus_tag="K0W68_000283"
     CDS             complement(69598..71133)
                     /gene="murJ"
                     /locus_tag="K0W68_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBI3071965.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(71243..72166)
                     /locus_tag="K0W68_000284"
     CDS             complement(71243..72166)
                     /locus_tag="K0W68_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBI3071966.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(72168..72815)
                     /locus_tag="K0W68_000285"
     CDS             complement(72168..72815)
                     /locus_tag="K0W68_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HBI3071967.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(72826..73410)
                     /gene="rimJ"
                     /locus_tag="K0W68_000286"
     CDS             complement(72826..73410)
                     /gene="rimJ"
                     /locus_tag="K0W68_000286"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBI3071968.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            73646..74854
                     /gene="mdtH"
                     /locus_tag="K0W68_000287"
     CDS             73646..74854
                     /gene="mdtH"
                     /locus_tag="K0W68_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBI3071969.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            74918..75565
                     /gene="grxB"
                     /locus_tag="K0W68_000288"
     CDS             74918..75565
                     /gene="grxB"
                     /locus_tag="K0W68_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBI3071970.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            75699..76259
                     /locus_tag="K0W68_000289"
     CDS             75699..76259
                     /locus_tag="K0W68_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI3071971.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            76365..77411
                     /gene="pyrC"
                     /locus_tag="K0W68_000290"
     CDS             76365..77411
                     /gene="pyrC"
                     /locus_tag="K0W68_000290"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBI3071972.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            77485..77730
                     /gene="dinI"
                     /locus_tag="K0W68_000291"
     CDS             77485..77730
                     /gene="dinI"
                     /locus_tag="K0W68_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBI3071973.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            78020..78274
                     /gene="bssS"
                     /locus_tag="K0W68_000292"
     CDS             78020..78274
                     /gene="bssS"
                     /locus_tag="K0W68_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBI3071974.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            78389..79507
                     /gene="solA"
                     /locus_tag="K0W68_000293"
     CDS             78389..79507
                     /gene="solA"
                     /locus_tag="K0W68_000293"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBI3071975.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        19596 a        20914 c        19709 g        19359 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgtcg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcac ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 cattgataat acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct
     2761 aaagttggat gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa
     2821 aagtacagag actatcccta tggatgataa atactgtcct tttattgcta cccataaata
     2881 atcaccaggc caatactatc aaattgatat ttgaaatgtg atcacttgac tttctatacg
     2941 ttattttata acggttaata tatttataaa aacaacgggt gtgccatacg cccgtttcaa
     3001 tacttaacgc acatgtgttt tggtttagtc atcatccagt tatatgtatt ttagccagga
     3061 acaggttaaa cctttcctat ataactcaaa aattgaaacc ttattctcat gtcatgttta
     3121 tattcattat tatcgttata taaaaaggca accataatgt ttagcaaatt ggcaccaagt
     3181 agcataaagg ctatgtttta attacaggat gttcagtcat ttgaatgtat aacattatag
     3241 ctaaacaaat ctaaaacgaa gtgaataatt tattgttttc actaaatctc attttgttta
     3301 acatccattg agattccttg ctttaaattt tgttttatat aagcaatcgt tttaattaat
     3361 ttattttttg aaggggtaat atactcatat gcaaaataaa gaaatgaaca tccaaatgaa
     3421 ctatattaaa taccgtggga taagacataa caaatgaagt gggtagtaat tgacacggta
     3481 attcaaccta catgcggcat atctttttca gccatatggg gtgatatgaa aaagatcatc
     3541 tggtatcaat ctactatatt tcttcctcct ggaagtatat ttacaccggt taagcttggt
     3601 attatcctta aggaaaaaga atatcctatt actatttata acattgcacc attcaacaag
     3661 aatttatgga gtttactcaa aagcagtcaa gagtgtcctc caggagaaag caaaataaca
     3721 aataaatgtt tacataatag ttgcattata aaaatatgcc catatgggct caagtaatgg
     3781 gcttcatata aatatgctcg atatgctatt aataatatcc acataaataa aacaacgggc
     3841 gtgttatacg cccgtttcaa tatttaactc atggagagat tacatgtttt cgatgatcgc
     3901 ttcaccaaac tctgaacatt tcagcagctt agcgccttcc atcagacgtt caaagtcata
     3961 agttacggtt ttcgcgttga tagcaccttc cataccttta acaattaaat cagccgcttc
     4021 agtccacccc atatggcgca gcatcatctc agcggagagg atgatagagc ctggatttac
     4081 tttgtcctga ccggcatatt tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc
     4141 gtcaccgatg tttgcgccag gggcgatacc gataccgcca acctgcgctg ccagggcgtc
     4201 agaaatgtag tcaccgttca ggttcataca ggcgataaca tcatattcag ccggacgcag
     4261 caggatctgt tgcaggaatg catcagcaat cacgtcttta atgacgatct ctttgccggt
     4321 gttcgggttt ttaactttca gccacgggcc gccgtcgatc agttcaccgc caaactcttc
     4381 acgcgccagc tggtagcccc agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc
     4441 tttgtgcacc agagtcacag agtcacgatc gttagcaatt gcgtattcga tcgctgcacg
     4501 aaccagacgt ttggtgcctt cttccgaaca cggcttaata ccgataccac aatgttccgg
     4561 gaagcgaatt ttcttcaccc ccatctcttc acgcaggaat ttaatcactt tctcggcgtc
     4621 ggcagagtct gctttccatt cgatacccgc ataaatgtct tccgagtttt cacggaagat
     4681 aaccatatcg gtcagttcag ggtgtttaac cgggcttgga gtgccctgat agtaacgtac
     4741 cggacgcagg cagatgtaga gatccagttc ctggcgcagg gcaacgttca gagagcgaat
     4801 accgccacca acaggagtgg tcagcggacc tttaatggca acgcgatatt cacgaatcag
     4861 atcaagggtt tcagcaggca gccagacatc ctgaccataa acctgtgtgg atttttcacc
     4921 ggtgtaaatt tccatccagg agattttacg ctcgccttta taggctttct cgactgcagc
     4981 gtcgaccact ttcagcatgg ctggggttac atctacaccg attccatcac cttcaatgta
     5041 agggataatc ggattttcag gaacgttgag tttgccgttt tgcagggtga tcttcttgcc
     5101 ttgtgccgga acaactactt tactttccat tcacctctcc ttcgagcgct actggtttgc
     5161 tcgtctgcca ccacgttgca tatgcgtttg cgtcctgcga tacggatgct ttagagcaat
     5221 tttttgttaa tgatttgtaa ttggcttgtc aatgctaccc tattgtttgc cgtcatgaaa
     5281 gatgcgcgtt attaggctat aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa
     5341 acttctttta gaaatcacca ggttaagcga ttcagctcgc aacgttctac taggcgtaaa
     5401 cctgaaaacc agcccacgcg tgtgatcctg ttcaataaac cctacgatgt tcttccgcag
     5461 ttcaccgatg aagccggacg caaaacatta aaagaattca tcccggttca gggcgtttat
     5521 gcagcaggtc gtcttgaccg cgataccgaa gggttactgg tgctgaccaa taacggcgcg
     5581 ttgcaggcgc gtttaaccca gccgggcaaa cgcaccggga aaatctatta tgtgcaggtg
     5641 gaaggtattc ccacacaaga cgcacttgaa gccttgcgca atggcgtaac cttaaatgat
     5701 ggccctaccc tgcccgccgg cgcggagctg gttgacgaac cagcgtggtt atggccgcgg
     5761 aatccaccaa ttcgtgaacg caaaagtatt cccaccagct ggctgaagat caccttatat
     5821 gaaggacgta atcgccaggt gcgccgcatg accgcccatg ttggtttccc cacgctgcga
     5881 ctgattcgtt atgcgatggg tgattacact ttggataacc ttgccaatgg cgaatggcga
     5941 gaagcaacag aataaggatg cacaatgttt aaaccgcacg ttaccgttgc ttgcgtagtg
     6001 cacgcagagg gtaaattttt agtcgttgaa gagacgatta atggtaaagc gttatggaac
     6061 caacctgccg ggcatctgga agccgatgaa accttagtgg aagctgccgc ccgtgagcta
     6121 tgggaagaaa ccggcatcag cgcgcaaccg caacacttta ttcgtatgca tcagtggatt
     6181 gcgccagata aaacgccgtt tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc
     6241 ccgacccagc ctcatgacag cgatatcgac tgctgtcgtt gggtcagcgc cgaagaaatt
     6301 ttaaaggcgt caaatcttcg ttcgccgctg gtggcggaaa gtatccgttg ttatcaaagt
     6361 gggcaacgct atccgctgga gatgattggc gattttaact ggccttttac aaagggtgtc
     6421 atctaaaagg ctgcgtgata gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa
     6481 tgtctgaaac cgcaaaaaaa gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt
     6541 ctgcctggct gttgcaacaa cagggatatc aggttgaagg cctgtttatg aagaactggg
     6601 aagaagacga cggtgaggaa tattgcacag cggcagcgga tctggctgat gcccaggctg
     6661 tctgcgacaa gctcggcatt gaactgcaca ccgttaactt tgctgccgag tactgggaca
     6721 atgtcttcga actgttcctt gccgaatata aagccggtcg cacgccgaat ccggatattc
     6781 tgtgcaacaa agagatcaaa tttaaagctt tcctcgaatt tgccgccgaa gatttaggtg
     6841 ccgattatat cgctaccggt cattacgtac gtcgggccga tgtcgatggc aagagccgcc
     6901 tgctgcgtgg tctggacagc aataaagacc agagctactt cctttatacg ctcagccatg
     6961 agcagattgc gcaaagcctg ttcccggtcg gcgaactgga aaaaccgcag gtgcgtaaga
     7021 ttgctgaaga tcttggtctg gtcaccgcga agaaaaaaga ctctaccggc atttgcttca
     7081 ttggcgaacg taaattccgc gagttcctgg gccgttatct cccggggcaa ccgggcaaaa
     7141 tcattaccgt cgatggcgat gaaattggtg agcaccaggg gctgatgtat cacactctcg
     7201 gtcagcgtaa aggtctgggt atcggtggca ccaaagaagg caccgaagaa ccttggtatg
     7261 tggtggacaa agacgtcgaa aacaacattc tggttgtcgc tcagggccat gaacacccgc
     7321 ggctgatgtc tgtcgggttg attgcccagc agttgcactg ggtcgatcgc gaaccattca
     7381 ccggcactat gcgttgcacg gtaaaaaccc gctatcgcca gaccgatatc ccttgcaccg
     7441 tcaaggcgct ggacgatgat cgcattgaag tgattttcga tgaaccggtt gccgccgtga
     7501 cgccgggcca gtctgccgtc ttctataacg gtgaagtgtg cctcggtggc ggtattattg
     7561 agcagcgtct gccgctgccg gtctgattat tatctttact taaaacaagg aagcagtgaa
     7621 cgtggcaaag aattactatg acatcaccct cgccctggcc ggtatttgtc agtcggcacg
     7681 cctggtgcaa caactcgctc accaggggca ttgtgatgcc gatgcgctac acgtctcact
     7741 caacagtatt attgatatga accccagctc gacgctggcg gtttttggcg gtagcgaagc
     7801 caacctgcgc gtcgggctgg aaaccctgct cggcgtgctc aatgccagca gtcgccaggg
     7861 cttaaacgcc gaattaaccc gctacacact cagcttgatg gtgcttgagc gcaaactctc
     7921 ctcagcgaaa ggcgcgctcg acactctggg caaccggatc aacggcctgc aacgccagct
     7981 cgaacacttc gatttacagt ccgaaacgct gatgagcgcg atggctgcta tctatgttga
     8041 tgtgatcagc ccgcttggcc cacgcattca ggtcaccggt tcccctgctg tactgcaaag
     8101 cccacaagtg caggcgaaag ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct
     8161 ctggcaccag gtcggcggcg gacgtctgca actgatgttt tctcgtaatc gcctgaccac
     8221 tcaggcaaaa caaattcttg ctcatttaac cccggagttg tgatctatgg aattatcctc
     8281 actgaccgcc gtttcccctg tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg
     8341 gattttcagc gaatatggtt tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca
     8401 aaaactggcc gcgcacgcag cgatcaagga agttcctgct tttgctgccg acgcaatcgg
     8461 ttaccttgat gcaattgtcg ccaatttcag cgaagaagat gccgcacgca tcaaaaccat
     8521 cgagcgtacc actaaccacg acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc
     8581 ggagatcccg gaactgcacg cggtttctga attcatccac tttgcctgta cttcggaaga
     8641 tatcaataac ctctcccacg cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc
     8701 gtactggcgt caactgattg atggcattaa agatctcgcc gttcagtacc gcgatatccc
     8761 gctgctgtct cgtacccacg gtcagccagc cacgccgtca accatcggta aagagatggc
     8821 aaacgtcgcc taccgtatgg agcgccagta ccgccagctt aaccaggtgg agatcctcgg
     8881 caaaatcaac ggcgcggtcg gtaactataa cgcccacatc gccgcttacc cggaagttga
     8941 ctggcatcag ttcagcgaag agttcgtcac ctcgctgggt attcaatgga acccgtacac
     9001 tacccagatt gaaccgcacg actacatcgc cgagctgttt gattgcgttg cacgcttcaa
     9061 caccattctg atcgactttg atcgtgacgt ctggggttat atcgccctta accacttcaa
     9121 acagaaaacc attgctggtg agattggttc ttccaccatg ccgcataaag ttaacccgat
     9181 cgacttcgag aactccgaag ggaatctggg cctttccaac gcggtattac agcatctggc
     9241 aagcaaactg ccggtttccc gctggcagcg tgacctgacc gactccaccg tgctgcgtaa
     9301 cctcggcgtg ggtatcggtt atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag
     9361 caaactggaa gtgaaccgtg accatctgct ggatgagctg gatcacaact gggaagtgct
     9421 ggctgagcca atccagacag ttatgcgtcg ctatggcatc gaaaaaccgt acgagaagct
     9481 gaaagagctg actcgtggta agcgcgttga cgccgaaggc atgaagcagt ttatcgacgg
     9541 tctggcgttg ccagaagaag agaaagcccg cctgaaagcg atgacgccgg caaactacat
     9601 tggtcgcgcc atcaccatgg ttgatgagct gaaataaact cgtatcagtg ccggatggcg
     9661 atgctgtccg gcctgcttat taagattatc cgctttttat tttttcactt tacctcccct
     9721 ccccgctggt ttatttaatg tttaccccca taaccacata atcgcgttac actattttaa
     9781 taattaagac agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta
     9841 cgtcaccacc ttaaagttca gattcaggat gctggtcatc aggtcgatga tgcagaagat
     9901 gccaaagaag ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc
     9961 ggattgccag acgaggacgg tctgtcactg attcgccgct ggcgtagcaa tgatgtttca
    10021 ctgccgattc tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt
    10081 gctggtgctg atgattacgt gactaaacca tttcatattg aagaggtgat ggcgcgaatg
    10141 caggcattaa tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct accaccgttt
    10201 caggttgatc tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg
    10261 ttcgaataca ccattatgga aacgttgata cgcaataatg gcaaagtggt gagcaaagat
    10321 tcgttaatgc tccaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta
    10381 ctgatgggac gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt gattactacc
    10441 gttcgcggcc agggctattt gttcgaattg cgctgatgaa aaaattactg cgtctttttt
    10501 tcccgctctc gctgcgggta cgttttctgt tggcaacggc tgcggtagta ctggtgcttt
    10561 cgcttgccta cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt
    10621 ttcggctgtt acgtggcgag agcaatctgt tctataccct tgcgaagtgg gaaaacaata
    10681 agttgcatgt cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt
    10741 atgatgagaa cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaagatga
    10801 tccagcctga ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata
    10861 ccagcctctt gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag
    10921 atgatgacga cgcggagatg acccactcgg tggcagtaaa cgtctacccg gcaacatcgc
    10981 ggatgccaaa attaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct
    11041 atatggtctg gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc
    11101 tgctgtgggt cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc
    11161 gcgaactgga agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca
    11221 gtctggtacg aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc
    11281 gtacgacgct caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta
    11341 cgctgcgttc tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg
    11401 agcaaatcag ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg
    11461 gcgggacatt gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct
    11521 cagcgctgaa caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag
    11581 agatcagctt tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata
    11641 atgcctgtaa atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc
    11701 tctatattgt ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt
    11761 tcgaccgtgg tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag
    11821 cccgcgaaat caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgctgggcg
    11881 gtgcgcggat ggaggtgatt tttggtcgcc agcattctgc gccgaaagat gaataaatat
    11941 gtccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat accagcctgt
    12001 ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt gaacgtcact
    12061 ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac ccgatctctc
    12121 cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga cagtcgactg gtcagtcacc
    12181 aggatggcaa atggcaggtc agccacggcc cgttcgaaag ctacgatcat ctcggtgaaa
    12241 ccaactggtc attactggta caggcagtga accactggca tgagccgacc gccgcgctga
    12301 tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatt tctttttctg
    12361 tacctggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc attcagggta
    12421 ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac tgcccacatc
    12481 cggatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg gagcctggtg
    12541 atattcttta tattccgcca ggattcccgc atgaaggcta cgcgctggaa aatgcgatga
    12601 actattccgt gggctttcgc gcgccaaata cgcgggaact gattagtgga tttgccgatt
    12661 atgtgctgca acgtgaactg ggcggcaact actacagcga tcctgatgtt ccacctcgcg
    12721 ctcatcctgc ggatgttctg cctcaagaga tggataaact gcgtgagatg atgctcgaat
    12781 tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc cagtcacgtc
    12841 atgaactgga tatcgcgccg ccagaaccgc cttatcagcc agatgaaatc tacgatgcgc
    12901 tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg
    12961 acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcactg gatgcactcg
    13021 ccagcaacat tgcgctgact gcggagaatt ttggcgatgc gctggaagat ccgtcattcc
    13081 tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt cttcgaaggg taagtttgga
    13141 tttaagccgg atgcggcatc acatgccgca tcccttttcg cttacttccg ttgcgccgtt
    13201 aactcggcaa tacggacgat cacctgcacc gctttttcca taccttccag agtcacaaac
    13261 tcatgcttac catgatagtt gtaaccgcca gtgaacaagt tcgggcacgg taatcccata
    13321 aacgacaact gcgcgccgtc ggtaccaccg cggatcggtt tcagttccgg ttcaatatcg
    13381 cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac cactttctct
    13441 cgcatattgt agtaactgtc ttcaatcacc agttcaatgt agcaatcagg atgtaaccct
    13501 ttgcccactt ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg
    13561 tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa cggtgccttt catgctcgcc
    13621 aggtgataga aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc
    13681 gcatgaatac gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccgga
    13741 tgaacattgt taccgacaat tttgatattg accgatgcgg cgttgaagtt ttcaaactcc
    13801 agttcgccta cgccaccacc gtcaacagtg taagcccagc gggcatcgaa ggcatcaaca
    13861 tcaaaatgtt tcgccccttt gcccacttct tcatccgggg taaaggcgac gcgaatatca
    13921 ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaatacct
    13981 gctttgtcat cggcacctaa caaggtttta ccatcggtgg taatcaacgt ctgacccagt
    14041 agctgatgca gcaccgggaa cataaccggt gataaaactt catcgccgat acccagcgca
    14101 atatcgccac cgcgatagtt ttcaacaatt tgcggattaa catttttgcc gctgcaatcc
    14161 ggtgaggtat ccacatgaga aataaagcca atcgccggga tatcgccagg gacgttagcc
    14221 ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatttct
    14281 tcgagctgct ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt
    14341 ctcacccctg cttttgattg ggtatccaga gatacgtagt tcaaaaatcg ctcaagtagt
    14401 ttatccatgt agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa
    14461 atattgcgtc aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat
    14521 gaatataaag ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta
    14581 gaattaccgc cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag
    14641 ccatccgttg cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt
    14701 caccgctggt gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc
    14761 agctggatct gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg
    14821 gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca
    14881 tgctggataa cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt
    14941 tccaaagcta cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc
    15001 gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg
    15061 tgcaactgga aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac
    15121 gcgtcgccat tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc
    15181 tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc
    15241 gtaagcttgg tattaccttc gtctttgtca ctcacgatca ggaagaagcg ctcaccatgt
    15301 cagacaggat tgtggtgatg cgcgagggtc gtattgagca agatggtaca ccgcgcgaaa
    15361 tttacgaaga gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatc aatatgttta
    15421 acgccactgt catcgaacgt ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg
    15481 aatgtaatat ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc
    15541 gcccggaaga cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg
    15601 gttacgttcg tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa
    15661 atggcaagat ggtgatggtc agcgaattct tcaatgaaga cgaccctgac tttgaccact
    15721 ctctcgacca aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg
    15781 aagaacacaa gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg
    15841 tttgtctttc tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc
    15901 agtttcgtca aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat
    15961 tttgaagtgc tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg
    16021 ctgggctacc cgtttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg
    16081 ctgtttctgc tgattgttcc cttctggacc aactcactga ttcgcatcta cgggctgaaa
    16141 attttcctca gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac
    16201 acaccaattc gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg
    16261 ctaccgttta tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt
    16321 gaggcggcgc gcgatctcgg tgccagcaag ttacagactt ttatccgtat cattattcca
    16381 ctgacgatgc cgggaattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg
    16441 ttctatgtat ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag
    16501 gtccagttcc tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact
    16561 atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt ctcgtctgct gaataaaaag
    16621 gtggaactcg aatgatcggt cgactgcttc gcggcggttt tatgaccgct atctacgcgt
    16681 acctgtatat cccaatcatt attttgattg tgaactcctt taacagctcg cgctttggca
    16741 tcaactggca gggttttacc accaaatggt atagcctgct gatgaacaac gacagcctgt
    16801 tacaggcagc gcagcattca ctaacaatgg cggtgttttc ggcgacattt gctacgctta
    16861 tcggttcact gacggcagtt gcgctgtacc gttatcgctt tcgtggtaag ccgttcgtta
    16921 gcggaatgct gtttgtggtg atgatgtcac cagatatcgt gatggcgatt tctctgctgg
    16981 tgctgtttat gctgctgggt attcagcttg gcttctggtc gctgctgttc tcgcatatca
    17041 ccttctgcct gccgtttgtg gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc
    17101 ggatgctgga agcggcgaaa gatctcggtg ccagtgaatt taccattctg cgaaaaatca
    17161 ttctgccact ggcaatgcca gcggtggcgg cgggctgggt gttaagcttt accctgtcga
    17221 tggacgatgt ggtagtttct tcgttcgtca ccggaccaag ttatgaaatt ctgccgttaa
    17281 aaatttattc gatggtcaaa gtcggcgtat cgccggaagt taacgcgctg gcaaccatat
    17341 tactggtgct gtcgctggtg atggttattg ccagccagct tattgctcgt gataaaacga
    17401 aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc
    17461 tctggcactg ggcatgagcg ccgctcacgc cgatgacaac aacacgctgt atttctacaa
    17521 ctggaccgag tacgtgccgc caggactgct tgaacagttc accaaagaaa ccggtattaa
    17581 ggctatctat tcgacttacg agtcgaacga aaccatgtac gccaagctga aaacgtacaa
    17641 agacggtgcc tatgacctgg tggttccttc aacctattac gtcgataaaa tgcgtaaaga
    17701 agggatgatc cagaagatcg acaagtcgaa gttaacgaac ttcagcaatc tcgatccaga
    17761 catgctcaac aagccgtttg acccgaataa cgactactcc attccgtata tctggggtgc
    17821 gacggcgatt ggcgttaacg gtgatgcggt ggatcctaaa tctgtcacca gttgggcaga
    17881 tctgtggaag ccagagtaca aaggtagcct gctgctgacc gacgatgccc gtgaagtgtt
    17941 ccagatggcg ctgcgcaaac tgggctactc cggtaacacc accgatccga aagagattga
    18001 agctgcatat aacgagctga aaaaactgat gccaaacgtc gcagcgttta actccgataa
    18061 cccggctaac ccgtacatgg aaggcgaagt taacctcggc atgatctgga acggctctgc
    18121 tttcgttgca cgccaggcgg gtacgccaat tgacgtggtg tggccgaaag aaggcggcat
    18181 tttctggatg gacagcctgg cgatcccggc aaatgccaaa aacaaagaag gcgcgctgaa
    18241 attgatcaac ttcctgctgc gcccggatgt ggcaaaacag gttgccgaaa ctatcggtta
    18301 tccaacgcca aaccttgcgg cgcgtaagct gttaagcccg gaagtggcga acgataaaac
    18361 gctctacccg gatgctgaaa ccattaaaaa tggtgaatgg cagaatgacg ttggcgcagc
    18421 cagcagcatt tatgaagagt attatcagaa gctgaaagca ggacgttaat tcagcaaacg
    18481 ggcggtggta ttaccgcccg ttttttagtt agcaaggatg tggctaatga gtcaggacag
    18541 taaagtttta tcccgctctt tcgtcggtat tgtagtcccc ttgtaggcct gataagcgta
    18601 gcgcatcagg caatgcttcc tgctttataa tcccttcagc aacttttcaa caaactcagg
    18661 caccacctgg cttgccgggc cgtaatattt ctcggcaaat tcattaccga cctgacttgg
    18721 ttcgagattc agttccacgg tgtgcgcgcc atgcagtttc gcttcgtgaa caaacccagc
    18781 cgccggataa acatgcccgg aagtaccaat ggcaatgaaa atatcagcca tcgacaacgc
    18841 catataaatt tcatccatgc cgagtggcat ttcgccaaac caaacgacgt gcgggcgcaa
    18901 gggtgccggg aactggcaac aatggcattt atcttctggc gtaacgtctc cggtccagtc
    18961 gagaacctga ccactttgcg aacaacgaac tttcagcaat tccccatgca tatgaatgac
    19021 attggtatta cctgcgcgtt catgcaggtt gtctatattc tgcgttacca gcaaaaagcg
    19081 atcgccgagg gcgtcttgca gtttagccag cgcaagatgc gcggcgttag gctgaatttc
    19141 tggctgctgc agctgtcgac gacgggcgtt ataaaacgct tgcaccagtt caggatcgcg
    19201 atcgaaacct tccggcgttg ccacatcttc aacccgatgt tcttcccaca ggccatctgc
    19261 ggcgcgaaag gtacgaatac ctgattccgc agaaattcct gcccctgtca gtacgagtac
    19321 tcttggtttt tccattgctt ccggcaccac tttatctctg aaaaaaatac gctgacgcaa
    19381 acgctcgcgc aggcggcgtt tatttttacg aaaacgactt aaccgatgac cccgacgcga
    19441 cagcatagca acctctgttg tttaatcggt tagatgtagg aaggccgcac cacgcattcc
    19501 tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca cgagctacag gtaagagatg
    19561 acgaggcagc ctgtccgcca gttgcgttgt gattgccggg aaattcgata agccaccacc
    19621 aatgacgacc aggtcagagt caacgatggt caggatattt cccagacaaa ccgctaataa
    19681 atccagataa cgctcaacgt gcgcccttgc ctgctcatcg ccttgatcat aaagcgcaat
    19741 aatttcggga gcctgcaacg gttgatgata atagtgttgc cagagccacg caaagccccg
    19801 acctgataga taattttcga tacaaccaat ttgaccacag ccgcagcggc gtaacgggaa
    19861 atccagcccc atcatggtta acgcatcaac cggcagacgc atatggccaa actcgccggt
    19921 aatgtagctt ttaccggtaa tcggtttacc gttgaaaatc agcccgccgc caacgccggt
    19981 gccgagaatt aaccccatca ccagtggata ttgcgtaaat tcatcatccc aggcttctga
    20041 aagggcaaaa cagttggcat cgttatcaag gcgtacatcg cgatcaagac gtgcgctcag
    20101 gtcggcacgc agcggtttac cgctggcagc ggggacattg gcggcataca gcgtaccatc
    20161 ttctgtttcc ggcattcccg gaataccgat gccaacagaa cctttacagc caaaacgttg
    20221 gtcagcttcg gctaccagct cgcatactgc atctaaaaat gcgtcatagc tgtcacgcgg
    20281 tgtcggcacc cgcttttccc actgcaactg ccgaccgcta tcaaacacgc caagcgcaat
    20341 ttttgttcca ccaatatcaa acccgtaata cattgccgct ccttgtttta atgtactgcc
    20401 tttactggcc gctaaggact cgcgcagggt caatattact ggcgcgccgc gccggatacc
    20461 aacttgccaa aagactcagc aacaatgctg tgaccagtac gtagaagacg tccagccagt
    20521 gcaattccga tggcaggaag tcaataaaat agatatcgct ggagaggaac tgatgaccga
    20581 tcagcttttc aatccgctca ataatcgggg taagttgcag tgaaacaact acgccgataa
    20641 tcacaccaca caggctaccg aacagccctg ccagcaatcc ataccagaca aagatggcgc
    20701 gaattaaacc atctttcgcc cccagcgttc ttaatactgc gatatcgcca ctcttgtctt
    20761 tcaccgccat cactaaggtg gagacgatgt tgaaacaggc cacgccaatc accagtacca
    20821 tcgccagata cataatggcg cggatcatct ggatatcgcg atacatatag ccgtaagtac
    20881 caatccaact tttaatataa acatagctgt tggtcacttc acccgcatcg cgtaccagct
    20941 tattggcgtt gaaaacatcc gtcattttaa gggcaatacc tgacacgctg gaacccatat
    21001 caagatattg ttgggcatcc gccagcggga tcatggcaaa actgtgatcg agttgaccac
    21061 tcaactgcaa aataccggca atgtgcaaac gcacacgttt tggctgcatc agtttatgct
    21121 cgggattcga gttgggaatc atgatcgaca cccaatcgcc ctgcttcact ttcagcgcat
    21181 ccgccacgcc tttgccgatg ataatttgct gttcgcccgc tttaaaattg cgccaggcat
    21241 ccccctgaac aaacgagggt aatgcgctca gacgctgttc ctgttgcggg ttaacgccct
    21301 tcacctgaat tgcgcgcaga ttggtaccac tttctaccag tccggtgaaa ttgatatacg
    21361 gcgcagcggc ggcaataccc ggcacttttt gcacgttatc cagtgcttcc tgccagttag
    21421 tccatggctg atttaccgcc tcaatttcac cgtgcggtac cacagcaaga atgcggttat
    21481 tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac gatcaatacc gccacgccga
    21541 gggcaatgcc aatggtagaa atgacagaga ttagcgacac catgccgccg cgtcgccgtc
    21601 cgcgactaaa acgcaggcca atcaataacg ataaaggcat cgccattact ccgcccccat
    21661 caggctcagt tccgccgtca gacgtccatc acgcatttcc agttggcggc tcatacgttt
    21721 cgccagttgc aggtcgtgag taaccaccag gaaggcggtg ccctgcaagc gattcaattc
    21781 cccaagcaac tggaagatgc tgtcggcgtt acgcgcatcg aggttaccgg taggctcatc
    21841 cgcaagtacc agacgcggat tattgaccag cgcacgggca atcgccacac gctggcgttc
    21901 gccgccagaa agttcagatg ggcggtgatt cgcacgatgc tccagcccca ccgcttttaa
    21961 catctccagc gcacggctgt tgatttcagc gggctttttc ttgccaatca gcagtggcat
    22021 agccacgttt tccagggcag taaaatccgg cagcaggtgg tgaaactgat aaataaagcc
    22081 cagcttctgg ttgcgcagtt ctgctttcgc cgccgaagac agtttgctca tcggttgacc
    22141 gttaaagatc acatcgccgg aggttggtgt atccagcccg cccagcaggt gcagcaaggt
    22201 acttttaccg gaaccagagc taccgacgat cgccatcatt tcaccttcgc cgacgctgaa
    22261 actgacattg tgcagtacat cggtttgcac actgccttcc tgatagcgtt tgcacaggtt
    22321 gtcgcattgc aacaggatct tattcataac gtaaagcctc agcgggttga gtggcggcag
    22381 cgcgccatga agggtaaagc gtagacagca gcgcgatagc catcgccacc agcgcaataa
    22441 caatgacctg taaaggttcg atagcgaccg gcagcgccgc gccatcaagc aggacgccga
    22501 ttatcggcat cagattattt aactggctgg caagcagtgc gccaagcgcc gctccgagga
    22561 tcgcaccgat aatcccggcg ctggcccctt gcaccataaa gaccatcatg atttgtcgcg
    22621 gagttaagcc ttgcgtttgc aggatcgcga cttcgccctg cttctccatt accatcagcc
    22681 ctagtgaggt aataatgtta aacgccgcaa cggcgacaat caggctcagc agcaagccca
    22741 tcatattttt ttccatgcgt acggcctgga acagctcgcc tttacggtca cgccagtcct
    22801 gccatttgct gccttcaggc agtttttgct gacttaaaga gtcaactttc agcggctcat
    22861 ccagccacaa acgccagccg gtaatattgc ctgccggata acgcatcaga cgcgaggcat
    22921 cctcaatatt caccagcatt tcatagccat cgacttcact gttggcggcg aaattgccaa
    22981 tcacattgaa caggcgctgg cttggaatac gccccatcgg cgtgaactgg ctggcagatg
    23041 gtaccatcac gcggatttga tcaccgcgat taacgcctag ctgtgaggca agttgttcgc
    23101 cgaggatgac attatatttc cccggctcga ggtcagtttg tttcacattg accagatacg
    23161 gcgtaagtgg atctttttgc gccgggtcga taccgagcat caccccgacc gccacgctgc
    23221 gcgcgctttg cagtaccaca tcaccggtag taataggtgc gacgcgatta acgccgtcca
    23281 gtttgactgc cgtttccggg agttgctgcg ggttaagaga gccatgctca gaagagagaa
    23341 ttgcctgtgg catcaggcca aggatgttgt tttgcagctc gcgctcaaag ccgttcatca
    23401 ctgacaatac tgtgaccagc gccatcaccc cgagggtaat gccgatggta gaaagccagg
    23461 agacgaaacg accgaagcga tccgctgcac gcccacgcat gtaacgcagg ccaataaata
    23521 gagcgacagg ttggtacatg aaatccgtct ggttgctgta gcaaagacac gagtatataa
    23581 gcgaaagcaa gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag
    23641 cccccgtaaa tcaatcggct aaattgccat gttttatcat caatcttgcc gcctgatctt
    23701 aatctgaaaa atcatccaat acagacagat acccattaca cctcgggtca taacccgcag
    23761 gatcaaccgc atgtaaatta aaaaagagcg tgttgtcctg atgaaacaaa aagagctatg
    23821 gattaaccag atcaaagggt tatgtatttg tctggtggtg atttatcact cggtcattac
    23881 cttttatccg catatgacca cttttcagca tccgttatcg gaagtcctga gtaaatgctg
    23941 gatctatttc aatctttacc ttgcaccctt tcgtatgccg gtttttttct ttatatccgg
    24001 ctatttgatt cgccgctata tcgacagcgt accgtgggga aattgtctcg ataaacggat
    24061 ctggaacatc ttctgggtgc tggcgctttg gggcgtggtg cagtggctgg cgttaagtgc
    24121 gctgaaccag tggctggcac ctgagcgcga tttaagcaat gcctccaatg ccgcttatgc
    24181 cgattccacc ggtgagttcc tgcacgggat gatcaccgcc agcaccagct tgtggtatct
    24241 gtatgcgtta attgtctatt tcgtggtatg taaaattttt agccgtctgg cgctgccact
    24301 attcgccttg tttgtactgc tgagtgtggc ggttaatttc gttcccacgc cgtggtgggg
    24361 aatgaacagt gtgatccgca atttgcctta ttacagcctt ggcgcatggt ttggcgcaac
    24421 aataatgacc tgtgttaaag aggtgccgtt gcgccgccat ctgctgatgg cttctttgct
    24481 taccgttctg gcggtcggtg cctggttgtt tactatctcg ctgctgttgt cgctggtatc
    24541 gattgtggtg atcatgaagc tgttttatca gtacgagcaa cgattcggta tgcgctccac
    24601 cagcctgctg aatgtgattg gctccaacac cattgctatc tacaccaccc atcgcattct
    24661 ggttgaaata ttcagcttaa ctctgcttgc gcaaatgaac gcagcacgct ggtcgccgca
    24721 agtcgaactg acactcctgc tggtttatcc ctttgtcagt ttgttcatct gtactgttgc
    24781 gggcttgctg gtaagaaaac tttcacagcg tgcattcagc gatctgttgt tctccccgcc
    24841 ttcactgccc gctgccgtca gttactctcg ttaaaccatc gccccagccg gggcgattca
    24901 atttgctaaa ccatgtcgat tacggataat gaacagcatt gcttatcaga gatatgcccc
    24961 catatgttga ggcatatcct aacgagaatc tgacaaccgt tatgcctgaa caatatcgtt
    25021 atacactgcc cgtcaaagcg ggtgagcagc gtctgctggg cgagttaacc ggcgcagcct
    25081 gtgcaacgct ggtagcggaa attgccgaac gtcacgccgg tccggtggta ctcattgcac
    25141 cagatatgca aaatgctctg cgtttgcatg atgaaatcag ccagttcacc gatcagatgg
    25201 tgatgaatct ggcggactgg gaaactcttc cctacgacag tttttcgcct catcaggaca
    25261 ttatctcctc gcgcctttcc accctttacc agctaccgac gatgcagcgt ggcgtactga
    25321 ttgttccggt gaatacgctt atgcagcgcg tttgcccaca cagttttctc cacggtcatg
    25381 cgctggtgat gaaaaaaggt cagcgcctgt cacgagatgc attacgaacc caactggaca
    25441 gcgccggtta tcgccatgtt gaccaggtga tggagcacgg cgaatacgcc acgcgcggcg
    25501 cgttgctgga tctcttcccg atggggagtg agctgcctta tcgtcttgat ttctttgatg
    25561 atgaaatcga cagcctgcgg gtgtttgacg tcgacagcca gcgcacgctg gaggaagtag
    25621 aagcgatcaa tctgctgccc gcgcacgaat ttccgaccga taaagcggca attgaactgt
    25681 tccgcagcca gtggcgcgat accttcgaag tgaagcgcga tccggaacat atttaccagc
    25741 aagtgagtaa aggcacatta cctgccggga tcgagtactg gcagccattg ttcttcagcg
    25801 aaccactgcc gccgctgttc agttatttcc ctgccaatac cttgctcgtg aatactggcg
    25861 atctggaaac cagtgccgaa cgtttccagg ctgacacgct ggcgcgtttt gagaatcgcg
    25921 gcgtcgatcc gatgcgcccg ctgttgccac cacaatcgct ctggctgcgg gtggacgagc
    25981 tcttctcaga gctgaaaaac tggccccggg tgcagctaaa aactgaacat ttaccgacaa
    26041 aagccgcgaa tgccaattta ggtttccaga aactgccaga cctggccgtt caggcacaac
    26101 aaaaagcgcc gctggatgcg ctgcgtaagt tcctcgagac tttcgacggt ccggtggtgt
    26161 tctcggtaga aagtgaaggt cgccgtgaag cgctgggtga actgctcgca cgaattaaaa
    26221 ttgctccgca acgcattatg cgtcttgatg aagccagcga ccgtgggcgt tatctgatga
    26281 ttggcgctgc cgaacatggt tttgtcgata aggtgcgtaa tctggcgctg atctgcgaaa
    26341 gcgatctgct cggtgaacgt gttgcccgcc gtcgtcagga ttctcgccgc accatcaacc
    26401 ccgatacact gatccgtaac cttgcggagc tgcatattgg tcagccggtg gtccatctgg
    26461 agcacggtgt cgggcgctat gccggaatga ccacgctcga agcgggcggc attactggcg
    26521 agtatttgat gctcacctat gccaacgacg ccaaactgta tgttccggtg tcgtcactgc
    26581 atctgattag ccgttacgca ggtggcgcgg aagaaaacgc cccgctgcat aaacttggcg
    26641 gcgatgcgtg gtcacgcgcg cggcagaaag cggcggaaaa agtgcgtgat gtggcggcgg
    26701 aattgctgga tatctacgcg caacgcgccg ccaaagaggg cttcgcgttt aaacacgatc
    26761 gtgagcagta tcagttgttc tgcgacagct tcccgtttga aaccacgccg gatcaggcgc
    26821 aggccattaa tgcggtactt agcgacatgt gtcagccgct ggcaatggat cgtctggtgt
    26881 gcggcgatgt tggctttggt aaaacagaag tggcgatgcg cgccgctttc ctggcagtag
    26941 ataaccacaa gcaggtggcg gtgttggtgc ctaccaccct tctcgcgcag cagcattacg
    27001 acaacttccg cgaccgtttc gccaactggc cggtacgtat cgaaatgatc tcccgtttcc
    27061 gcagcgccaa agagcagacg caaatccttg cggaagtggc ggaagggaaa atcgatattc
    27121 tgatcggtac gcacaaactg ctgcaaagtg acgtcaagtt taaagattta ggcctgctga
    27181 ttgtcgatga agaacaccgc ttcggggtgc gtcataaaga gcgcattaaa gcgatgcgcg
    27241 cgaacgtgga tattctgacg cttactgcaa cgccgatccc acgcacgctg aatatggcaa
    27301 tgagcggaat gcgtgacctg tcgattatcg ccacgccgcc cgcccgtcgt ctggcagtta
    27361 aaacctttgt ccgtgagtat gacagcctag tggtccggga ggcgatcctg cgtgaaattt
    27421 tgcgcggggg gcaggtttat tatctctaca atgatgtgga aaacatccag aaagctgccg
    27481 aacggctggc agaactggtg ccagaagcgc ggatcgccat cggtcacggg cagatgcgcg
    27541 agcgcgaact ggaacgggtg atgaatgatt tccatcatca acgtttcaac gtgctggttt
    27601 gtacaaccat tatcgaaacc gggatcgaca tcccgacagc caacaccatt atcattgaac
    27661 gcgcggatca cttcggtctg gcgcagctgc accagttacg cggtcgcgtc ggacgttcgc
    27721 atcatcaggc atatgcatgg ttgctgacac cgcatccaaa agcgatgact accgatgcac
    27781 aaaaacgtct tgaagcgatt gcctcgctgg aagatctcgg ggcaggtttt gcgctggcaa
    27841 cgcacgatct ggagattcgc ggcgcgggtg aactgcttgg cgaagaacaa agcggctcaa
    27901 tggaaaccat cggtttctcg ctgtatatgg agttgctgga aaacgccgtc gatgcactga
    27961 aagccggacg cgagccgtcg ctggaagatc tcaccagcca gcaaacagaa gtcgagctgc
    28021 ggatgccgtc gctattgcca gatgatttca ttcctgacgt gaatacgcgt ttgtcgttct
    28081 ataaacgtat tgccagcgcc aaaacggaaa acgaactgga agagatcaaa gtcgagctta
    28141 tcgatcgctt cggcctgctg ccagatccgg cgcgtaccct gctggatatt gcccgtctgc
    28201 gccagcaagc gcagaaactg gggatcagga agctggaagg taatgagaaa ggcggggtga
    28261 tcgaatttgc cgagaagaat cacgttaatc cggcctggtt gattggtttg ctgcaaaaac
    28321 agccgcagca ttaccgcctt gatggtccga cgcgcctgaa atttattcag gatttgagtg
    28381 agcggaaaac gcgtatcgaa tgggtacgcc agtttatgcg tgaactggaa gagaacgcga
    28441 tcgcttaatg gtaaatcggg ggcgtttctg cgcccccatc tgcaacattt acaaattctt
    28501 tgcacttccc tgcactatcc gacactgtca ccatccataa ttcaggctta tctgtttatt
    28561 acaataacct tatatttatt attgattttt ggtgatgatc aaaacgcgtt tttctcgctg
    28621 gctaacgttt tttacgttcg ccgctgccgt ggcgctaccg gcaaaagcca acacctagcc
    28681 gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa tttcatgtgg tggaaaatga
    28741 cggtggttct ctggaagcca tcgccaaaaa atataacgtc ggctttctcg ctctgttaca
    28801 ggctaacccc ggcgttgatc cttacgtacc gcgcgcgggc agcgtgttaa cgatcccgtt
    28861 gcaaacccta cttccagacg cgccgcgcga aggcattgtg atcaacattg cggagctgcg
    28921 tctctattac tacccgccgg gtaaaaattc ggtaaccgtg tatccaatag gtattggtca
    28981 gttaggtggt gacacgctga caccgacaat ggtgaccacc gtttcagaca aacgtgcaaa
    29041 cccaacctgg acgccaacgg caaacatccg cgcccgttat aaagcacagg gaattgagtt
    29101 gcctacggta gtgccggctg gaccggataa cccaatgggc catcatgcga ttcgtctggc
    29161 ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc gatttcggca ttggcatgcg
    29221 ggtaagttct ggctgtattc gtctgcggga tgacgatatc aaaacactct ttagccaggt
    29281 caccccaggc accaaagtga atatcatcaa cactccgata aaagtctctg ccgaaccaaa
    29341 cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag aagattgatg acgatccgca
    29401 gctgctgcca attacgctga atagcgcaat gcaatcattt aaagatgcag cacaaactga
    29461 cgctgaagtg atgcaacatg tgatggatgt ccgttccggg atgccggtgg atgtccgccg
    29521 tcatcaagtg agcccacaaa ctctgtaaat cctggataaa aaaaatcccc gcgacacggc
    29581 atgtcgcggg gaaaaaatca gcagtagcag gcattaatga gggttaatgc ttatttataa
    29641 attactgctg ttccatggag ggtattcgga ccggttacag aagtaatacg gaaagatttt
    29701 gcgcccatct catccgcttt ttgcgccagc tgctcttcca gcgatcccag atttgtcccc
    29761 gcgttagcac tgatagtacc gactttttgt tggccttctg gcgttgactg aacttcgaca
    29821 gccgcaaagt tggcaaatga catggagctt aaaatcgccg cagcgatgag ggtttttacg
    29881 tttttcataa tagtggcctt atgcagatga atgacgtcgc aagaaattac ttaacgatcg
    29941 ataactaaat gatagatgtg atctggatca catacaagat attttttata acaatcatta
    30001 attaatttaa aaatctttaa atttcagtta aatagaaagc aattattttt atgggatctg
    30061 gactggtgaa cggaggtgat tatttttata ataacctttc attcacagtc accaggttca
    30121 acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag
    30181 tgttcgacag ggatgccgcg cttgataagg ccatgaaatt gttctggcaa cacggttatg
    30241 aagcgacttc tcttgcggac ctcgtcgaag cgaccggagc caaagcgccc acgctatacg
    30301 cggaatttac caacaaagag gggttatttc gtgccgttct cgaccgctat atcgatcgtt
    30361 ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg
    30421 ctgactattt tgctgccatc gccaactgct ttaccagcaa agacaccccg gcaggctgct
    30481 tcatgatcaa caactgcacc accctctccc cagattcagg agatatcgcc aatacgttga
    30541 aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc
    30601 gcggggaaat cccgacccac tgtgacgtga cacatctggc agaattcctt aattgtatta
    30661 ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct ggaaaaactg atgcagattg
    30721 ccggaacgac tttgcgttta tggcccgaac tggtgaaata agtcgcaaaa aaagcccctc
    30781 agcctgagcg aatgaggggc caaattgcag agtacagcct tgttatactt tgttattcaa
    30841 aatcagttgc ccgttactat ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat
    30901 tttgccctgc tgatcgccaa tcttataggt cacatcataa ccgagcattt tttctgactt
    30961 gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga
    31021 gccctggatc tggttaccgg catatccacc acccagcgcc cccacaacag tggcgacatc
    31081 tttaccacga ccaccaccaa actgatgccc tatcacgccg ccagcaacag cgccgagcac
    31141 cgacccggta atgcgatttt catcctgcac cggtcgacga tgggtcactg tgacgttgcg
    31201 acactcctga cgcggtgttt taaccgtttc cttgattggg gttgcagaaa caacctgagc
    31261 gtattgcggg ccccgttcaa acacgttcag actggccact gccgctacgc ccagcgcagc
    31321 tgcgacacca atcccgatac ccgccaacat tgatttattc acgggaacac ctccttcttt
    31381 gcagttatgc caatcatgca agcgaaggag aaaagcagct atcagacaga agatgaaaag
    31441 cgcgggaatg gctggaaata cttaaggatt cggagaagtc tgaaggaatg cctgatgcga
    31501 cgcttaacgc gtcttatcag gcctaccgct cgtaccattt gtccagggcg gatgaggcgt
    31561 ttatgccaca tccgccagtg tacgtcgatt aatgcaactt caaacgcgga cggataacgc
    31621 ggttaatact ccccaccagc atcattaatc cggttttaaa gtaaccatgc agcgcaatct
    31681 gatgcattcg gtatagcgag atatatacaa agcgcgcaat tcgtccttca atcatcattg
    31741 agccgcgcgt caggttaccc atcaggctac cgacagtgga gaagttcgac agcgatacca
    31801 gcgaaccatg atctttatac tgataatttt tcagcggttt accgttcatc tgcgccagaa
    31861 tatttttcat tgcgcaggtc gccatctggt gtgcagcctg agcacgcggc ggaacaaagc
    31921 ccccttccgg acgcgggcat gacgcgcagt cgccaatagc gtaaatgtct ggatcgcggg
    31981 tggtttgcag cgtcggttcc accaccagct ggttgatacg gttggtttca agaccaccga
    32041 tatctttcag gaagtctggc gctttgatcc cggctgccca caccatcaga tcagcctcaa
    32101 tatattcacc atctttagtg tgcaggccgc cttcatcagc actggtgacc atggtttgcg
    32161 tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc cgcagcagag atacgtggcg
    32221 gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg
    32281 tcaggccttt gtaaccgtag ctgtgcagtt gcttgaccgc attgtacaat tcagcggaga
    32341 gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt cactttacca ttcgcgccca
    32401 ggttggcgga gtatttcagg aacagattca gcatctcctg gtggaagcga cgcgcctggt
    32461 gcgggttatc gaggaaaatg cagttctctt tgacacctgg cgtattgaaa tcgttagagg
    32521 tgctacccag cgccattacc agggtgtcat aggcgatttt acgttccgga accagcagtt
    32581 cacctttctc atcgcgcagt tctgcaatag tgattgtttt cgcttcacga tcaatatcaa
    32641 tgacggaacc cagctggaac tggaaaccat gattgcgcgc atgggccaga tagctcaacg
    32701 catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg cagcagcggt ttccacagat
    32761 ggctgtggtt acgatcgacc agcgtaattt tggctttttt cttgcgtccc agcttatgcc
    32821 ccagctgtgt tgccatttcc agcccaccag caccgccgcc gacaatcaca atttttttca
    32881 atggcgtagt caacgtgacc cccttaaatt tattaaccaa ttgttaatta atggttataa
    32941 acatagctct taattaacaa caggttacag caatgaaaat gttcttcgct attgagaata
    33001 acatgaatgg tgcattggtc ataccaaaat tgatgttaat caagttttgt tgctgaaaag
    33061 ataggcgact ttaccgggtg agatcaaaaa acagagttta ttgtttatta tttgagcaag
    33121 tccggaaggg tgatataggg tgacgcgtat atcgagggcg tttggtcgtc ctgataagat
    33181 gcgcagcatc gcatcaggca ttgatgctcg actgtcggat gcggcgtaaa cgccttatcc
    33241 gacttacaga attcggagcg catcaggtac cggcaaggtt acctgatgcg acgagggcat
    33301 ttacccgagg gttttgaacg ccttaatgcg ctgcaaatgc ggggagatat tcttgaattt
    33361 gtgcgtctgc tcttcgtccc agacaatctc gtaataatga tgcaactctt cagatgtccg
    33421 ctggctgtta agcgcttcat cattacgcga caaaatcacc aggcaacgat cgcgattctt
    33481 ctcgcggaag ttggtcacac acttagtggc aatatcggca tactcttccg ggcgatcaat
    33541 cttgccttcc atgttctcat aagggaacaa attagggttg aagatcacct ggcggatatc
    33601 gcaaagaaaa ccaatccgtt ccgcccagta tccgcccaga ccaacgccgc aaatcagcgg
    33661 acgctcgtcg acgttcagtt gcaacatttt gtccacttct ttgagcagat gctgcatatc
    33721 atgtttcgga tgccgcgtac tgtagcttat caagcgtaca tccgggtcaa taaactgcaa
    33781 ttgtaagact ttctcgtggt tacccggact gttagagtca aaaccgtgta aatagataat
    33841 catcgcttcc tcacataagc cagggttagt gacctgcttt ctcttcctgc cagcgttcat
    33901 gtaactgatt cagacgggtg ctgatcgcct tccagcgagc cgagtccatc agttcctgtc
    33961 gcgaaaatga acctttatga tacaaacgtg taacacgttc tgccttgatc ggtgacagat
    34021 tatctaacac gctgacggcc cctttacgat tattgcagac caggatcata tcgcaacctg
    34081 cgtccagtga tgcctgcccg cgttcggcat aactgcccat aatcgcggca ccttccatcg
    34141 ataaatcgtc agagaaaatt acaccgtcaa aacacagttc ctgacgcaaa acggttttca
    34201 gccagtagga agaaccgctc gccggacgcg gatcaacatc actgtagatc acatgcgcag
    34261 gcataatggc gtcgagttta ttttcgcgga ttaaggaact gaagaccgac atatctttag
    34321 cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta
    34381 ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat tccggcttca tgcataccat
    34441 caataaaccg gctggcaatt gccagggctt tttgtggatc ggcatgataa gaacgctcgc
    34501 caatcgccgc gctgatatgc ccgacatcca gcactggcgc aaagctgata tcaatatcca
    34561 tagcgatcat ttcgctggcc atcagccaac ccgcctcttg cgccagtttg ccgccctctt
    34621 ccattcccaa cagcgcagca aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa
    34681 aacgctgcac gcgtccacct tcctgatcta ccgccaccac cagatgattg cgcgatgctg
    34741 cgcggatctg gcgcaccagt tcacgtaact gggcaggatc atgatagtta cgcgtaaaga
    34801 gaatcagccc tcccaccagc ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt
    34861 aaccttcgac atccaacatt actggaccca cactgctctc cttattgttt tattaatagc
    34921 tgccgccagg tgtcatcggc cagcctgata aattgttgat cgccgatttg tcgccagcgg
    34981 tactcaaacc accctgcttt gagcatcagc agccagggaa accatcgcct gacctgacgc
    35041 cataattgcg ccggataaat cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc
    35101 tgttcagtat tttccaccca caccgccgcc agttccagcg cgatatcacc atctccggca
    35161 tactcccagt cgatgagttt taaccctgac gcgctatgca ctaaatttcc ggcgtggaca
    35221 tccatatgca atggacttaa gcgtaaaggc cgtggttccc gcgctttgcg cagacgtttt
    35281 aacattcgca gccaacccac agtccgccgc gccggatcgc tttgctgcca gtacagttcc
    35341 agtaagggca acagcgttat tcgccagcca aaacgtggtt gttgatgaag ataatacagc
    35401 aagcctgcca gttcgttggt atccggcaaa tacgttttta ccgcgccggg cagatagtcg
    35461 actaccatcc agtcacggag atataaatgc ggcttcggtg caatgcttgc gggtagttgt
    35521 gataaagccc gatactggcg caagaacgcg gactgcggcg catcaggatc gtgcggctga
    35581 cgcacaacaa aacgctggcc ctgatgttca atgagaaaac taccgccact aagcccacta
    35641 ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg
    35701 atgggattat tgctgcgaaa cggcaccttt acctgaccag ataatttcgc ccgtctgcac
    35761 cagcatcagc tgcatttgta gggtcggagc cttaacgttg ccagaggcgc tggagtacag
    35821 cacgtaatga gcgccgacat tgcgggcaat gcctatggct ttactacggg tacctaaact
    35881 gtcctgcggc gacaaaccta actgttgctt agccatcgac agctgctggg cggaaaccag
    35941 ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt
    36001 cagcgaaccg ttagtacggt tgttaacgct atcaaccagc aggacgctac ccgcagtcac
    36061 cccgtcagcc ccaagcatct tactgaccat cggctgcatt gcgccattcc agtcataatg
    36121 gcgaatatgc ggcgcaggcg gtgccgtttg atcttcgtgc tcaattgggc ctggctgctg
    36181 cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt tgtggctcgg ctggttgttc
    36241 cggcgctggt ttcacttctt ctaccggtgc aggttcacgt tgccccacac acccggcgag
    36301 aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt tttgtcatca agattcaccc
    36361 cttacaaata tagataaagt ctgactttgt gcgcccccag aaaattggcg ctgccgtaca
    36421 gcgttaccgc cgaatgtgcg ggaatgctaa cgctgcgtgg cctttccaga ggatgcatct
    36481 ccagccctct ggcgtcatac cagtaaaaac gataatgaac ggtaacgggt tcttgccttt
    36541 cgttataaag cgttgaggat gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt
    36601 ctgcactgat gcccgcagcc aataaagatg actccatcac cagcgattgc tcatcattca
    36661 ccggaatttc cggatgtgaa cgacagccca ccagcagtaa caacgccaga gacaccagcc
    36721 caaagcatcc ttttctcatc gttacagacc tttatgcgcc agcattggtc ccagcggacg
    36781 gccacccaac aagtgcatat ggatgtggta aacctcttgt ccgccatggc ggttggtatt
    36841 catgatcaga cgatagccat cttcggcaat accttcttgc tcagcaattt ttgccgctac
    36901 ggtgatcatg cgtcccagcg cctgctcatg ctcagctgac acgtcgttca cagtcggaat
    36961 gaggatattc ggaatgatca gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc
    37021 cgttaccaga tcatcctggt agacgatatc ggaggggatc tcacgacgaa taattttgct
    37081 gaatatagtt tcttctgcca cgacattttc ctttttcaaa tagccatgcg ttgcgccatg
    37141 ctacgccgcg ccacactgcg agtatagagt atggtcgagt taaacctgct ctttcaacct
    37201 taactcacga tttcttaagc aaaaaatcgt tttgctgccg tatatatcgc cattattccc
    37261 atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt ggtttacacg acaaatatga
    37321 atgcgcatat ttctcatttg catttacaaa caaaattatt cgcacactaa atataaccgc
    37381 tcatcaccct cctccggcat gagcctgacg acaacataaa ccaagagatt tcaaatgctt
    37441 tcaacacaat ttaacaggga taatcaatat caagccatca ccaaaccgtc actacttgcc
    37501 ggttgcatag cactggcact attaccttct gccgcttttg ctgcaccagc cactgaagaa
    37561 acggtgattg ttgagggttc agccacagct ccagatgatg gcgaaaatga ttacagcgta
    37621 acgtctacct ctgcgggtac caaaatgcag atgactcaac ttgatattcc tcagtcggtc
    37681 actattgtta gccagcagcg gatggaagat cagcagttac aaacgctggg cgaagtgatg
    37741 gaaaacacgc tggggatcag caaaagtcag gcggattccg atcgtgctct ttattattcc
    37801 cgtggattcc agatcgataa ctatatggtt gatggtatcc ccacctattt tgaatcgcgc
    37861 tggaatctgg gcgacgcact ttctgatatg gcactctttg aacgcgtaga agtagtgcgt
    37921 ggcgcgacag gactcatgac cgggacgggt aatccatctg cggcaattaa tatggttcga
    37981 aaacacgcga ccagtcgtga atttaaaggc gatgtctcgg cggaatacgg tagctggaac
    38041 aaagaacggt atgtggcgga tttacaaagc ccactcaccg aagacggtaa aatccgcgcg
    38101 cgaattgtcg gcggctacca gaataacgac tcatggctgg accgctacaa cagtgaaaag
    38161 accttcttct cgggcattgt cgatgctgat ttaggcgatc ttacgacgct ttcagccggt
    38221 tacgaatatc agcgcattga tgttaatagc ccaacctggg gcggtttacc gcgctggaat
    38281 actgatggca gcagcaacag ttacgatcgt gcacgcagta ccgcacctga ctgggcgtac
    38341 aacgataaag agatcaacaa ggtctttatg accctgaagc agcggtttgc tgatacctgg
    38401 caagcgacac tgaatgccac ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc
    38461 gatgcctatg taaacaaagc ggatggtatg ctggttgggc catacagtaa ttatggacct
    38521 ggctttgatt atgtcggcgg caccggttgg aacagtggca aacgtaaagt tgatgcgctg
    38581 gatttgttcg ctgacggtag ttatgaattg tttggtcgtc agcacaatct aatgtttggt
    38641 ggcagttaca gcaaacaaaa caatcgttac ttcagttcat gggccaacat cttcccggat
    38701 gaaattggca gtttctacaa ctttaatggc aatttcccac aaaccgactg gtcaccacag
    38761 agcctggcac aggacgatac cacacatatg aaatcgttat atgctgctac tcgagtcacc
    38821 cttgccgatc cgctgcatct gatcctcggc gcacgttata ccaactggcg ggttgatacg
    38881 ctgacttaca gcatggagaa aaaccacacc acgccttacg ctggtctggt gtttgacatc
    38941 aatgacaact ggtcgaccta cgccagctat acctctattt tccagccgca aaatgatcgt
    39001 gacagttcag gcaaatatct ggctccaatc accggtaaca actacgagct gggtctgaaa
    39061 tcggactgga tgaatagccg tctgaccacc acgttagcca tcttccgtat tgagcaggat
    39121 aatgtcgctc agtccaccgg tacacctatc cccggcagca acggcgaaac cgcctataaa
    39181 gcggtggatg ggacagtcag taaaggggtg gaatttgaac tcaacggcgc aattaccgac
    39241 aactggcagc tgacatttgg cgcaacgcgc tatattgcag aggataacga aggaaacgcc
    39301 gttaatccta atctgccacg caccacggtt aaaatgttca ccagctatcg gttgcctgtc
    39361 atgccagagt tgacagtcgg cggtggtgtt aactggcaaa atcgcgtgta taccgacacc
    39421 gtgacaccgt atggcacctt ccgcgccgag caaggtagct acgcgctggt ggatctcttc
    39481 acccgctacc aggtgacgaa aaacttctcg ttacagggga acgtcaataa cctgttcgac
    39541 aaaacctacg ataccaacat ggaaggttct atcgtctacg gcgcaccgcg taatttcagc
    39601 attaccggca cgtatcaatt ctgaataaca cctgtaaaaa aggcagccat ctggcggcct
    39661 tagtctcccc aacgtcttac ggattagtgg ttacggatgt actcatccat ctcggttttc
    39721 aggttatcgg atttagtacc gaaaatcgcc tgaacaccag aaccagcaac cactacgccc
    39781 gctgcgccca gtttcttcag gccagcctga tccactttag acacatcagc aacgctgacg
    39841 cgcagacggg taatacatgc gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca
    39901 accagagccg gtgccatttc gctggtacct gttgcttttg catcttcagt cgcgtcttca
    39961 cgacccggcg ttttcagatc cagtgcttta atcagcacgc ggaagatggt gtagtaaaca
    40021 atcgcataac cgataccgac gatcgggaac agccacagtt tgctgctgtt accagacaga
    40081 acgataaagt cgatcagacc gtgcgagaac gacgtaccgt cacgcatccc cagaagaata
    40141 cagattggga atgccaggcc tgccagaatc gcgtggatga tgtacaggat cggcgcaacg
    40201 aacatgaagg agaactcgat cggctcggtg ataccggtca ggaacgaggt cagcgccgcg
    40261 gagatcataa taccgcccac tttcgcgcgg ttttctggtt tagcagagtg ccagatagca
    40321 attgcggcag ctggcagacc gtacattttg aacaggaagc caccagacag tttacccgca
    40381 gtcgggtcac ccgccatata acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg
    40441 gtgtattcac caatctgcat ctggaaaggt acgttccaga tgtggtgcag accaaacggc
    40501 accaggcaac gttcgatgaa accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca
    40561 gcccactgag agaaggtctg gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca
    40621 acgccagtaa agatggcagc caggccagaa atgatcggca caaagcgttt accggcaaag
    40681 aagccaagat actcaggcag cttaatacgg tagaaacggt taaacatgta cgctgcgatc
    40741 gcaccggaga taatccctcc gagtacgcca gtatccgcca ggtgtttaga ggcgatttct
    40801 tcagcaggta aatgcagtac cagtggcgca accacggcca tggttttaac catgatgcca
    40861 taggcaacaa ctgcggccag cgcggatacg ccatcgttat tggtaaagcc gagggcgaca
    40921 ccgatcgcaa aaatcagtgg catgtttgca aagacggaac cgcctgcttc tgccataaca
    40981 tgcgatacaa cggcgggcag ccagctgaaa ttcgcggaac cgacgcccag cagaatacct
    41041 gcgataggca gtacggatac cggcagcatc agcgatttac cgaccttttg caggttagca
    41101 aatgcattct taaacataat tgagagtgct cctgagtatg ggtgcttttt ttacgttctc
    41161 acgcgtggca aggggggaga gcctcgccgt gtacagggca tctaagcgcc ctttatttat
    41221 tacacagagt aaaataattc agtgccgatt tgtttgacgg ctatcacatt tcaacttcag
    41281 atcattggag aagttgcaca ttttgacaaa acatatgtca caatatttca aacgtggtta
    41341 ttcaccgccc aattttcggc ggtgaacttt actcgtttta gccattaatt acgagcttta
    41401 aaaaaactca ttcaacggat ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg
    41461 aagttatcgg tggttacctg cgccagttct tcaacggcaa cacctttcaa cacagccatg
    41521 tattctgcaa cgtcacgaac catcgcaggt tgattctctt ttcctcgatg cggtaccggc
    41581 gcaaggtaag gtgagtcagt ttccaccagt aaccgatcca gggggacata acgcgcagca
    41641 tctcgaagtt gctccgcatt acggaaggtc acaatgccgg aaaaggagat gtaaaatccg
    41701 agatccagta atttacccgc cgtttctctg tcctctgtaa aacagtgtag tacgccaccg
    41761 caatccgtca ctttttcttc gcgcagaatt gccagcgtat cggcgcgggc gtcacgggta
    41821 tggacgataa ccggcttgtt cagttcacga ccaatctgga tatgatggat gaaggactct
    41881 tgctgacgaa ctttagtttc cggtgtgtaa taataatcca gcccggtttc acccagcgct
    41941 acaacaccct cttctgccgc cagacggcgt aaatcttcta catcgtaggg atcattctgg
    42001 ttgagtggat gcacgccaca agaaaatacg acgttgtcac gttcgcctac cagatcccgc
    42061 atatgtaaat aacccggtaa tgttgtggcg actgccagac aaaatttcac atcgcgtgcg
    42121 gcggctttcg ccagaacgtc atccacgtcc ttatgcaaag attcataatc cagaccatcg
    42181 agatggcagt gtgagtcgac taaaaacatg atgtctctct taaagatgag gaaccggtag
    42241 cacaacgccc ggttgcaggt aatgctcaat acgcagtaaa agatcggtga tgagaagctc
    42301 gcggttgatg cctgtgacag acattaactg ttcacgaatg tggcaaacat cccccagtat
    42361 agcctgcagg cgcgagggag aaagatggtt tgccagttcg acgacaaggc ccggcacatc
    42421 aacattggtc acctgcgcag caccatgatg gcgttttagc gcatccatca gcaacgttgc
    42481 cagccagtgt aaacgcgccg gagcttgttc atgattaagg gccgccagca gcgaatacca
    42541 gtcgcccgat ggcacgctat atgccaatgc ctgacacaat gtttcacgag cctgccagtt
    42601 atctccctga aacaacgcca gtgccgcgcc aggcgaaccg gcgcttaagc gcaatgcggc
    42661 aagtaatgca tcctgtgaca ttgtcacttc gcgtgaaagc caggtcacgg cgtactgttc
    42721 cggcggcggc gcaaggtaat gtaaccgaca acgactacgt aatgttgcca gtaaacgttc
    42781 aggctcgcgg gtagccagga aaaaccaggt ttctgctggt ggctcttcaa gcgttttcag
    42841 caatgcgtta gccgcggcgt cggtcagtaa ggcagcatcg gttacccaga caactttcgc
    42901 accacctagg cgtgcgtgct cattcagctt ttcggtgacc tcacgtactg catcaatgcc
    42961 cagcgtattt ttcccttttt cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg
    43021 catcaactga catccacgac agtgaccgca acttttgtgg ccctgcggtt gttggcagag
    43081 taaataacgg ctcagggcgt agattaaagc atcatcgccc atgcccggta acgcctgaat
    43141 gagtagcgca tggtgacctc ttccggcctg atagctggct accagttttt cgaaatcagg
    43201 tcgtaaccat ggataccatc tcatgcgtcc aactccttca cccagtgggt cacggtagtg
    43261 cggattgcat ccatcacggc ctccagcggc tgggtggcat caatggtatg aatactttta
    43321 tcttgtgcag ccagttccag atagcgggcg cgggtgcgat taaagaaatc gaaagactct
    43381 tgctcaatac gatccagctc gccgcgcgca cgcgcgcgtt ttaagccaac ttccggggta
    43441 acatcgagat agagcgttaa gtcggggcga aaatccccga gaacagcatc acgcagtgtt
    43501 gccagcatat gttggtcaat accacgtcct ccgccctgat acgcctgagt ggagagatcg
    43561 tggcgatcgc caatcaccca ggtgccgtta gccagagctg gtttgatgac cgtttctacc
    43621 agttgaacgc gcgcggcata aaacatcaga acttcggctt tatcggtaat gacttcatcg
    43681 cctaccgatt tgatatccag caccaggctt cttaactttt cggcaagttg cgtaccgcca
    43741 ggttcccgag tgaaaaccat gtcgcggata cccagttgct caagcgtctc aaccaccaca
    43801 ttacgcgcgg tagttttgcc tgcgccttcc agcccctcaa tgacgatata cttactgcgc
    43861 atttttttcc ttaagcactt tcagataatc ctgcacagac ttgttatggc tggcaagatt
    43921 ggtattaaac gtgtgaccac ctttaccatc ggccacaaaa tagagatacg gcgtttttgc
    43981 cggatgcgca gcagccttca gcgaatccgc ccccggcgta gctatcgcac ccggcggcaa
    44041 accggtaatg gtataggtgt tatacgctgt cggcgtttcc aggtctgcac gagaaagttt
    44101 gccattataa cgctctccca tcccgtaaat cacggttggg tcggtctgca ggcgcatacc
    44161 aatgcgtaaa cggttgataa ataccgaggc aacctgatcg cgttcactgg caacggcggt
    44221 ttctttttcg ataattgatg ccatcgtcac cagctgattt ttatctttat aaggcagacc
    44281 gtccgcacgc ccttcccagg cgctatcgac cgctttcacc attttcttgt gcgctcgctt
    44341 gagtaacgcg acatcggtgg tattggcggt atacatccag gtgtctggcc agaaccaacc
    44401 ttcaatccac tccgggtttt ccagttcaag tgcctgcgct acggtggcgt acttatcgtc
    44461 actcagcgtg tgcttgatat acggagcctc acgcaattgc ttgaggtagt cgctcagacg
    44521 catcccttct accagtcgca gcgggaactg tgcttcttta ccgctttcca gcaatttcag
    44581 catctcgcgc acggtcatct gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag
    44641 atccggttcg atacgcagca accattgaaa aacccgtggg cgattgatga tcttatcggc
    44701 ataaagctgt tcaccaagcg ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc
    44761 ttctttgata agcaatttgc tgtcggcaag atggcgaacc ttccagacgc ccacaccagc
    44821 ggcgataccc agtaccacca gcaataacaa gataatcaat aacacttttt tcatgactaa
    44881 ttcgggcgct cacaaagtgg ggctaaatat tcatataacg ttgctgacga aaaggagaca
    44941 tcgccacagg cacgtacggg catcactggc attaacgcat tacaaataac catctcatct
    45001 gcctgcaacg cctcttccag agaggcttgc acttcgacaa gctgataaga ggattgtgcc
    45061 agcaaacgga tacagaattg tcgcataatg ccgttaacac ctgcctgatc cagacgcggc
    45121 gtataaacta cattgccctt ccgccagaac aaattagccg cacagcattc cgtaacccac
    45181 ccttcgctgt caaggaccag cgcctcatca gcgtttgtct gctcaagatg agagcggatc
    45241 aatacttgct caagccgatt aagatgttta atacctgcaa gatgaggatt gcgccccagc
    45301 cgcaccgggc ttagcgccaa cgtcatccct tcgttacgca aacggtcgta atgtgcagga
    45361 taaaccgtaa cggagagaat ccgcgttgcc ggtccactgt tcaatgtgct gtaccctcgc
    45421 ccgccactac cgcgactgat cacgactttc agcacaccat tttgctgttc tgctgccagc
    45481 gttttcatct cttgttcaag ctgaggccag aagtcacaga aaatcatcaa ccgctgacaa
    45541 gcatcctgta gtcgctggat atgcgccgat aacaaactga ctttaccgtc gataactctg
    45601 gcggtggtaa aacaaccatc accaaactgc gttgcccgat cgcttactgc cagcgattcc
    45661 tgcttatgac cgttaattaa gaacataccg gctccttatc agagaattgt tagtgtggca
    45721 gcatgttcac tacggaacaa gtcggaataa aagctaagaa aaaaggcccg caagcggacc
    45781 ttttatatgg gtgggaaatg acaacttaga tctttttaaa gatcaaagaa ccattagtgc
    45841 caccgaagcc gaaggagtta cacagcgtgt attccattcc gctgacctga cgtgcttcat
    45901 gcggtacgaa atccagatcg caaccttcat ccgggttatc caggttgatg gttggcggaa
    45961 cagcctgatc gcgcagcgcc aggatggagt agatagattc tactgcaccc gccgcaccta
    46021 acaggtgacc ggtcatagat ttcgtggagc ttaccaacac acggcttgca gcttcaccga
    46081 agatggtttt caccgcctgc gcttcagctt tatcgccagc cggcgtagaa gtaccgtgcg
    46141 cgttaacgta gccaatctga ctcgcttcaa tgcctgcatc acgcagagca tttgccatcg
    46201 ccagagctgc gcctgcgcca ttttctggcg gtgacgtcat atgataagca tcgctgctca
    46261 taccaaagcc gacgagttca gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact
    46321 cttcaagtac cagcataccg gcaccatcgc ccagtacgaa gccatcacgc tctttatccc
    46381 acgggcggct cgccgcttgc gggttatcat tgcgggtaga taatgcacgt gccgcgccaa
    46441 aaccaccaac acccagcggc gtactggctt tctctgcgcc acctgcaacc atcacgtcag
    46501 catcgccata cgcgataata cgcgcagcat ggccaatgtt gtgcacgccg gaagtacagg
    46561 cggtcgcgat agagatgctt gggccacgca ggccatacat gatagtcaga tgacctgcca
    46621 ccatgttcac aatcgttgac ggaacgaaga atgggctgat cttacgtgga ccaccgttca
    46681 tcagagatgt gtggttttct tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg
    46741 caccaatgcg ggttgcgttc tcttccgtta tatcaaggcc agaatcctgc atggcctgaa
    46801 cgccagcgac aattccatat tgaatgaagg catccatctt gcgctgttct ttgcgcgaga
    46861 taatgtcctc acagttaaaa tcctttacta agccagcaaa tttcgttgca taggcgctag
    46921 tatcgaaatg gtcgattagg ctgatgccac tctgaccggc aagcagagct ttccaggtag
    46981 actctacggt attgccgaca ggagacaaca tgcccagtcc ggtcacaact acacgacgct
    47041 tagacacgtt tgtcctccag ggagggaaaa aatgattcta gtgggacaaa aagataaaac
    47101 tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc ctggtggccg ttgatgtaat
    47161 caatggcagc ctgaacggtg gtgattttct cagcttcttc gtccggaatc tcagtatcaa
    47221 actcttcttc cagagccatt accagctcaa cggtgtcaag agaatccgcg cccaggtctt
    47281 caacgaaaga agcattgttg gtaacttctt cctgcttaac gcccagctgt tcgccgataa
    47341 ttttcttaac gcgttcttcg atagtgctca tactcttaaa tttcctatca aaactcgctt
    47401 tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt gcaactaaat cccggcaggt
    47461 cttaccacga ttttacgtta ttttgaggcc ttacccccca ataacgcaaa tcattttgca
    47521 ctaattatta ctcataacca cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg
    47581 gtcagaccat gtacatcccg ccgttcacat gcaaagtttc acccgtgatg taagctgctt
    47641 cgtcggatgc caggaatgca accgcgttgg cgatttcctg tgcgccgccg aggcgacccg
    47701 caggaacctg cgccaggata cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg
    47761 tttcaataaa gcccggagca acaacgttta cagtaatacc gcgtgacgca acttcgcgcg
    47821 ccagtgattt actgaagccg atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc
    47881 catttcccat ggtaccaacc acagaaccga tagtgataat acgaccatga cgctttttca
    47941 tcatagcgcg cattaccgct tttgacagac ggaaaacaga tgaaaggttg gtttcgataa
    48001 tatcgttcca ctcttcatct ttcattcgca ttaacaggtt atcacgagtg ataccggcat
    48061 tattgaccag gatatccact tcaccaaatt ctgcgcgaat tttttccaga acagattcga
    48121 tagatgccgg gtcggtcaca ttcaacatca gacctttgcc gttggcacct aaataatcgc
    48181 tgatcgcctg agcgccattt tcactggtcg cagtgccaat aactttcgcg ccacgggctg
    48241 cgagcgtttc agcaattgcg cggccaattc cgcggcttgc accggttacc agtgcgattt
    48301 ttccttcaaa attcatgatt ttcctctttt aaagctcaag cgccgctgcc atcgctgaag
    48361 gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca
    48421 ccttgcccgg gccgacttca tagagatgtt ctacgccttg cgctgccatg tactcaacag
    48481 acttcgtcca ctgaaccggg ttatacaact gacgtaccag tgcgtcacgg atggcatcac
    48541 cattggtttc gcatttcaca tcaacgttat tcacaacaga aactgttggt gcgttaaagg
    48601 tgattttcgc taattctact gccagtttgt cggctgctgg tttcatcagc gcacagtgag
    48661 acggtacgct cactggtaac ggcagagcac gttttgcgcc cgcagcttta caggcagcgc
    48721 cagcacgctc aaccgcttct ttatgaccgg caataaccac ctgtcccgga gagttaaagt
    48781 ttaccggaga aacgacctga cctgctgcag cttcttcaca cgctttcgca atagacgcat
    48841 catccagacc gatgattgcc gccatagcgc ccgtaccttc cggtacggct tcttgcatga
    48901 gcttgccgcg catctcaacc agacgcaccg catcagcgaa atcaatcaca ccagcgcaaa
    48961 ccagcgcgga atattccccc aggctgtgac cggccatcat tgccggtgct ttaccgccct
    49021 gctgctgcca tacgcggtac agcgcaacag aggcagtcaa cagcgctggc tgggtttgcc
    49081 aggttttatt cagttcttca gctggcccct gctgggtcag cgcccacagg tcgtagccca
    49141 gcgccgcaga agcttcagca aacgtttctt cgacaattgg atagctcgcc gccatatcag
    49201 ccagcattcc aacggtttga gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt
    49261 tttaatcctt atcctagaaa cgaaccagcg cggagcccca ggtgaatcca ccgccaaagg
    49321 cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg
    49381 cgcacgggac agaggccgca gaggtattac cgtggcgatc cagcgtcacc acgacattgt
    49441 ccatagacat accgagtttt ttcgccgttg cactgataat acgcaggtta gcctgatgcg
    49501 gaaccagcca gtccagttga gaacggtcaa gattatttgc cgccagcgtc tcatcaacga
    49561 tgtgcgccag ttccgttacc gcaaccttga agacttcgtt gcccgccatc gtcagatgaa
    49621 ttgaattctc tggattcaca cggtcagcat taggcagcgt cagcaactca ccatagctac
    49681 cgtcggcatg cagatgggtg gagatgattc ccggctcttc agaggcagcc agcaccgcag
    49741 cgcccgcgcc atcgccaaaa ataataatag tcccacgatc ggttggatcg caggtgcgcg
    49801 ccagtacatc ggaaccgacg accagagcat acttcaccgc cccagatttc acgtattgat
    49861 cggctacgct taatgcatag gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc
    49921 aacctttaat gcccagcatg ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag
    49981 cagaagtcgt tgccacaacg atcaggccaa tctggtcttt ctcaatgccc gccatctcaa
    50041 ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt
    50101 ggcgttcgcg gataccggta cgagtgacaa tccactcgtc agaggtgtcc accatttttt
    50161 ccaaatcggc gtttgtccgc acttgttcgg gcagatagct gccagtacca ataatcttcg
    50221 tatacatgta cgctcagtca cttttcggtt atataccgtc acttgcaaac tgcgagttcg
    50281 ctggcagcgt cctgctaccg cagagttccg cttttgccac cgtccagcag ctcaaaacca
    50341 gctgggtata cagattccag gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc
    50401 tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat tggctgcacc atgacttttt
    50461 atcaccgtgc cgcgcaatcc taacagacag gcgccgttat actggtcggg gttgaggtga
    50521 ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta ataacagtag ccaccacgac
    50581 cgttttttcc cttcaccctg agatttcagc agagaaagga acatcctgac aacaccttcc
    50641 atcgtcttta atgtgacatt tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca
    50701 gttaacaact cattggcttc aagatagccg atataattga tagaagggat tgttttaagc
    50761 accgctgagg catcccgaat actgtcgaga ccctttactt cttcttcacc aatattgagc
    50821 aacgccacgc gaggattggg aatttccacc acctcttcag ccagaactga gcccataatg
    50881 gcaaattgca ccaacattgt actgtcacaa tcgacgttgg cccctaagtc aaggaccacc
    50941 gttttgccct tttgctgatg tggtaatacc gtcaccagcg ccggacgctc aatcccctcc
    51001 aggggcttga gtaataattt tgccagcccc atcagtgccc cggtattacc ggcactgaca
    51061 caggcttgcg ctcgaccttc tttcaccagc tccagggcca cgcgcattga actcccacga
    51121 ctggcgcgga tagcttgcga aggccgggca tcactggcga taactgactg cgcaggaata
    51181 atctgcagac gcgaacgttg ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg
    51241 ggattgccga ctaaaagaag agtgagttgc gaattagaat tcagtgcctg caatgctgca
    51301 ggcactgtca cggaagggcc aaaatcccct cccatgacat ctaacgccag ggttagacgt
    51361 gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt tgcctgggga aaatcctcac
    51421 taagcttcat cacgcagacg cgtgattact tagcgatgac cttgcggccg cggtagtaac
    51481 cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga agttttgtct acagacaggc
    51541 tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc acgtttggaa cgggttggtt
    51601 tattctgttg tacggccatg gaccttactc ctcaattacg gtttatcccc gatccaacgg
    51661 ggagcaccaa ttacttacgc tttaagctgg ctaatacggc aaatgggttt ggcttttgca
    51721 cttcttcagg cagttcacca aagaccatgt ccgcttcgga cacttcacag tgttcagaat
    51781 catgcaccgg aactaccggc aaggcgagga tgatttcatc ttcaaccatt gcaagcagat
    51841 cgatttcacc gaattcgtta acctcaatcg gttcatacgc ttccggcagt gcttcagcct
    51901 gttcgtctga acgcacagga ctaaaacaat acgttgtgta gacctgatga gtaaacggct
    51961 tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc atcgccgttt aacactgcga
    52021 gacgttggtt atcgatagcg aacgacatgg agcattccac atcactgtcc acactgacta
    52081 cggattcggc gacgcgctca acctgatcag gggtatagat accctggtaa tcaaggcgtt
    52141 tttgagccgt acgaaccgga tcgagagtca ggggtaattt taccttttgc atagggcgcg
    52201 catattaact ttgtaacgtc atagagtcaa agaaaaaggc agcctgtggt tgccttttgc
    52261 caataattcg cacacattgc gggttactgc tttattttcg ttcagtgctg ataacgaaca
    52321 tcaacctgca aaacgaagcg catagtttaa aatggctatc atcaatacgc tatatctgat
    52381 ggaaaaaaat atgcctaaac ttattttagc ctccacctcg ccatggcgtc gcgctcttct
    52441 ggaaaaactg caaatctctt ttgaatgtgc agcaccagag gtcgacgaaa ccccacgcag
    52501 cgacgaatca ccgcgacagt tggtgcttcg actggcacaa gaaaaagcgc aatctctggc
    52561 gtcacgttat ccggatcatt taattattgg ttcagaccag gtatgtgttc ttgatggcga
    52621 aatcaccggt aaaccgttaa cggaagaaaa tgcccgtctg caattacgca aagccagcgg
    52681 caatatcgtc accttctata ctggactggc gttgtttaat tcggcgaatg ggcatctaca
    52741 aacagaagtg gagccttttg acgtccattt ccgtcacctg agcgaggcgg agattgataa
    52801 ttacgtgcgt aaggagcatc ccctgcactg cgcgggtagc tttaagagtg aaggatttgg
    52861 cattacgctg tttgagcgtt tagagggacg tgaccctaac acgctggttg gtttaccgct
    52921 aatcgcgctg tgtcagatgt tacgtcggga agggaaaaac ccgctgatgg gatagttgtt
    52981 tgcgctggat gcggttacgt cgcatccggc aatcggtgca ggcggcctga tgcggcgtaa
    53041 acgccttatc aggccaaccg cccattatca ggcttatatg cttgtattag cgcgcgttac
    53101 gcagcttttg caaacaacgc ttcaaacctt catccatcgg cgcttcgata cgcatcacct
    53161 caccggtccc cggatgggta aacttcaacg ctgcggcgtg caggaacaga cgatttaatc
    53221 ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg
    53281 caatcggatg acccgcatac tgtgtatgca cacggatctg atgagtacgt ccggttaccg
    53341 ggctacaacg caccagggtg gcaaatgcat agcgttcttc cactttaaag cgtgtttccg
    53401 acggtttgcc ttcctgactg acacgcacga tacgttcgcc gctttgcaga atatttttca
    53461 ataacggcgc ttgaacgctc ttcacatgcg actgccactg accgcgcacc agcgccaggt
    53521 aatctttttg cattcctttt tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt
    53581 ttttcgctat cagcaacaca cctgaagtgt cccggtcgag acgatgaacc agttcaagga
    53641 accgcgcttc cgggcgcaac gcccgcaaac cttcgataac gccgaagctt aaaccactgc
    53701 cgccatgtac cgccgtaccg gaaggtttat tcagcaccag gatatgatca tcttcatata
    53761 agatgacgtc cgccagcgcc gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc
    53821 gctcagcaac gcgaaccggt gggatacgca cctcatcacc cgcttcgagt ttatattcag
    53881 gcttaatacg ttttttgttc acccgcactt cgcctttacg caaaatacgg taaatcatac
    53941 tttttggtac gcctttcaat tgggtacgca aaaagttatc gatacgttgc cccgcttcgt
    54001 cagcggtgat agcaacaatt tttacggatg gagtctctgt tttcatggtg ggcgattcta
    54061 attagccaac aggattcgcg ccactcattt ttctatgctt atatttagtt ttgcacctta
    54121 ttacttcact acgtgatcac tttattgatg gttattaaac caatcaccag caagaagtga
    54181 aaaaactgtg agtaagcggg tgataaatgg taaaagtcat cttgctataa caaggcttgc
    54241 agtggaataa tgaggccgtt tccgtgtcca tccttgttaa aacaagaaat tttacggaat
    54301 aacccatttt gcccgaccga tcatccacgc agcaatggcg taagacgtat tgatctttca
    54361 ggcagttagc gggctgcggg ttgcagtcct taccggtaga tggaaatatt tctggagagt
    54421 aatacccagt ctgtttcttt gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg
    54481 acactctgcg cctctttgag ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga
    54541 ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag
    54601 aataatgagt aagttacgat gaaaagaatg ttaatcaacg caactcagca ggaagagttg
    54661 cgcgttgccc ttgtagatgg gcagcgtctt tatgacctgg atatcgaaag tccagggcac
    54721 gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa
    54781 gctgcttttg ttgattacgg cgctgaacgt cacggtttcc tcccactaaa agaaattgcc
    54841 cgcgaatatt tccctgctaa ctacagtgct catggtcgtc ccaacattaa agatgtgttg
    54901 cgtgaaggtc aggaagtcat tgttcagatc gataaagaag agcgcggcaa caaaggcgcg
    54961 gcattaacca cctttatcag tctggcgggt agctatctgg ttctgatgcc aaataacccg
    55021 cgcgcaggtg gcatttctcg ccgtatcgaa ggcgatgacc gtaccgaatt aaaagaagca
    55081 ctggcaagcc ttgaactgcc ggaaggcatg gggcttatcg tgcgcaccgc tggcgtaggc
    55141 aaatctgctg aggcgctgca atgggattta agcttccgcc tgaaacactg ggaagccatc
    55201 aaaaaagccg ctgaaagccg cccggctccg ttcctgattc atcaggagag caacgtaatc
    55261 gttcgcgcat tccgcgatta cttacgtcag gacatcggcg aaatccttat cgataacccg
    55321 aaagtgctcg aactggcacg tcagcatatc gctgcattag gtcgcccgga tttcagcagc
    55381 aaaatcaaac tgtataccgg cgagatcccg ctgttcagcc actaccagat tgagtcgcag
    55441 atcgagtccg ccttccagcg tgaagtccgt ctgccgtccg gtggttcgat tgttatcgac
    55501 agcaccgaag cgttaacggc catcgacatc aactccgcac gcgcgacccg cggcggcgat
    55561 atcgaagaaa ccgcgtttaa caccaacctc gaagctgccg atgagattgc tcgtcagctg
    55621 cgcctgcgtg acctcggcgg cctgattgtt atcgacttca tcgacatgac gcctgtacgc
    55681 caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt
    55741 caaatcagcc atatttctcg ctttggcctg ctggaaatgt cccgccagcg cctgagccca
    55801 tcactgggtg aatccagcca tcacgtctgc ccgcgctgct ccggtaccgg taccgtgcgt
    55861 gacaacgaat cgctgtcgct ctctattctg cgtttgatcg aagaagaagc gctgaaagag
    55921 aacacccagg aagttcacgc cattgttcct gtgccaatcg cttcttatct gctgaatgaa
    55981 aaacgttctg cggtgaatgc gattgaaacg cgtcaggacg gtgttcgctg cgtgattgtg
    56041 ccaaacgatc agatggaaac cccgcactac cacgtgttgc gcgtgcgcaa aggggaagaa
    56101 acgccgacct taagctacat gctgccgaag ctgcatgaag aagcgatggc gctgccgtct
    56161 gaagaagagt tcgctgaacg taagcgtcct gaacaacctg cactggcaac ctttgccatg
    56221 ccggatgtgc cgccagcgcc aaccccagct gaacctgccg cgactgtcgt agccccagca
    56281 ccgaaagctg caacggcaac accagcagct cctgcacaac ctgggctgtt aagccgcttc
    56341 ttcggcgcac tgaaagcgct gttcagcggt ggtgaagaag ccaaaccgac cgagcaacca
    56401 acaccgaaag cagaagcgaa accggaacgt caacaggatc gtcgcaaacc tcgtcagagc
    56461 aaccgccgtg accgtaatga gcgccgcgac acccgtagtg aacgtactga aggcagcgat
    56521 aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc agcagactgc cgagacgcgt
    56581 gagagccgtc agcaggctga ggtaacggaa aaagcacgta ccaccgacga gcagcaagcg
    56641 ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac gtcaggcgca acaagaagcg
    56701 aaggcgctga atgttgaaga gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt
    56761 ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc
    56821 gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt
    56881 gttcaggaag ctccagctcc acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg
    56941 caaactgcac cagaacagca agaagagaac aatgctgata accgtgacaa cggtggcatg
    57001 ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc
    57061 tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct
    57121 ccggaactgg cctctggcaa agtctggatc cgctatccaa ttgtacgtcc gcaagatgta
    57181 caagttgaag agcagcgcga acaggaagaa gtacaagtgc agccgatggt gactggggtc
    57241 cctgtcgccg ccgctgtcga acctgttgtt agcgcaccgg ttgttgaaga agtggctgaa
    57301 gttgtagaag cccccgttca gattgccgaa ccgcaaccgg aagtggttga aacgacgcat
    57361 cctgaagtga ttgccgccgc agtaactgaa cagccgcagg tgattaccga gtctgatgtt
    57421 gccgtagccc aggaagttgc agaacacgca gaaccggtgg ttgaaccgca ggaagagacg
    57481 gcagatattg aagaagttgc cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt
    57541 gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag aagttgaaac ggtagctgcg
    57601 gttgaacctg aggtcaccgt tgagcataac cacgctacgg tgccgatgac gcgcgctccg
    57661 gcaccggaat atgttccgga tgcaccgcgt cacagtgact ggcagcgccc tacttttgcc
    57721 ttcgaaggta aaggtgccgc aggtggtcat acggcaacac atcatgcctc tgccgctcct
    57781 gcgcgtccgc aacctgttga gtaataatta gctcaagtaa tcaagccctg gtaactgcca
    57841 gggctttttt atttcatctt tgaatcaaca tattaaaatt catcgaatgg catccttgct
    57901 aaccaacaat gcaaaatagg caaaaaaatg cccgccccgg cgatcccaga gcaggcagac
    57961 aaaacatacc cagtttcatg atatgtttta aagcgaaatt atttgttgag ctggaagagc
    58021 gacaatccct gcatatcggt aaatgcttta tacgatgcct gcaatgccgt ttgctgcatg
    58081 atgtaagatg aaatagttgc attccagtcc acatcaacca gatcgctcat ctgctgcgtt
    58141 tgccctaaag cgcgatcgct acctaatgaa tccagcgact ccagttcgtt cagctgcgtg
    58201 cctaattccg cgcgaacggt cagcacattg ttcagcgagt ttttcagtcc gcggttggtt
    58261 ttatctaacg ccgcagcggc ggtttcttta tccgcttcgc tatccgcgac cggcgttttc
    58321 agggctgcga tggcgctatc cagcatggca aaaagattgg tttcagaagc gctaccgtct
    58381 ggttccgcta ccgcgttact ggtaatactg tcgaaaattt tgtcgcccgt atgtcctatc
    58441 accatcgaac gcgaagcatc gacctgttgc ttaatacttt ctgttccgcc gacataatca
    58501 ccgttagcct cactaaacgg cgcagtctct gttttataac cggcaaaaat gtagcgccca
    58561 ttaccgtcag tggtgttcgc cagattcagc aactggtcac gaagcccctg aatatccgta
    58621 gccagcgagg cccggtcatc gtcactcaag gtgccattgc tggcgtagac aattttttcc
    58681 tgagcattct ggatagcagt ggtgacctgg ctaagtacac tctcttccag tgacactttt
    58741 tgagtggcga aagtacgcgc cagcgtgtac tggctgtttt gcgcctgtgc ctgggagaga
    58801 acaaccgctt gtgatgcagc aatgggatca tctgaagggt taacgactcg cttacccgtc
    58861 gacatctgtt cgccgtactt catccattct gcctgagaat tggtgatacc acgcatgttt
    58921 tgctggtaca tcatctgggt actgaaacgc attttatctc cccttagcga atgttaatca
    58981 gcgcatcaaa aatcgcgttt gccgtctgca gaacctgcgc attcgccagg taatactgct
    59041 gaaaacgttg cagatttccg tactcctcat cgagattgac accggaaatc gactgctgct
    59101 gattggaaag ctgcgtcacc acattacctt gcgtggtgct actggttttc aacgtcgcgg
    59161 ttttattacc gatatcactc actaacgagg cataagcgtc gttaaaggat ttcgcaccgc
    59221 ccaccgtttt actattgctt tgcagatcca gcagggcctg accgttgcgg ttatcgctat
    59281 cacccgcatc tttttcgctc gccatcgcta ttttcgcttc gtcggtgatt aatacatcca
    59341 tgttgacgat ggcgtcactt actggtttca gcgtgaagct gtcgttaacg gcaggcgttc
    59401 ctgtaaacgt caactccaga ccatcaaatg ccactttacc gttggcatcc ggcgtcaccg
    59461 taaaagtggt attgctggca aggcgggtga cctgccactg attattatcg aacgagattt
    59521 tgtaatctgt cgccagtacc acggaggcat cagttaccgt ggcaccgatc gcaacgtcac
    59581 ctttgttttt cgtgttttgc agaaccgcgg gcttaccgat agtaaagaaa tcttcaccag
    59641 catcgccgtt agcatcaaat ccggctttgt gttgggtgtt gaaagcctcg gcaaatgcca
    59701 gcgccagttg tccaagcgta ttacgcgtct ggtccagctc ctgagaacgg aatgtcagaa
    59761 tgccgcccag cgacccggta ttcagtaatt tctccgggat ctcaatattg cctgccgtcc
    59821 catcaacata agcgacagtc gtacgagaag ggtcagcgct ggaaggaact gccgccagtt
    59881 gccgcgccgt acttccctga accagtgagt aaccattggc caccgtgatg ttataagtgc
    59941 cgccatcctg aacgctgact tctacaccaa caatctggtt taattcgctc accagttgat
    60001 cgcgttgatc cagcagattg ttaggtgacg cccctgcccc cacgcctgtc aggcgcgaga
    60061 tttgatcgtt caggctggca atttgtttag cgtaattgtt gatctgatca acgctggcac
    60121 ctatcgcgat attgacctgt ttgtcctggt cgcgcagata ttgatcggtg gttttaaact
    60181 gattcaccaa cccttctgat ttcccaatca gcgcctggcg cgctgccggg tcttccgcgt
    60241 tactcaccag cgtttgcagg ctggtgaaga aatcctgcat ctgtgttgcc agcgaagagg
    60301 tactggtgga gagcatattg tcgattttcg acatctgctc atagcgggcg gtcaggccgc
    60361 tactttgcgt ctgcgcagca cgtaactggt tggtaataaa cgcatcatac tcacgctgca
    60421 caccagaaac gtagacgcca ttgccaaccc agccgccagc gcccaacgtg ctattggcct
    60481 gcgccataat agtggtttgg cgggtatatc cggcaacgtt atagctggag atattattac
    60541 ttgccgtatt taacgccgcc tgggccgcgt tcagtccgct catggcgtta ttgatcaagc
    60601 tggacatgga ggttccttat aagccttcaa ttacagagta ttatcggcag cgacccgccg
    60661 gacttgagtt attcagaaca gattatcaat gttcatgctg taggttttgc tcaccttgtc
    60721 gcttatcgat ttcatctgct ggatcatgtt ggtgagttta cgggcatagt gaggatcggt
    60781 agcatacccc gcgtcctgta gggcctgcgc cccctgctcc gcactcgcgg ctgtcgtcac
    60841 ggcggcgtag cgcgggttac gtgttaacag cccaacgtaa tccgacaatg cttccagata
    60901 cgagctgtag acccgaaatt tcgcttttac tttcttcgct tcgccatttt catattcagt
    60961 cgtggtgatt tcagtaactg gacctttcca gttgccagag gctttgacgc caaacaggtt
    61021 atagctcggc tcgccgtttt cgcggcggat ttgccgttgc ccccaaccag actccagcgc
    61081 cgcctgagcg aggatcaaat gatgtggcac accgctttgc tggcttgcca gttgggcggg
    61141 cagcgagagt tgcgcgagga atgctttact gtcacccggc agcgaatcat cgtagttacg
    61201 tggcacggcc ttttgcacca gctgcgaaag cgcctgattt tgataacgca ccacagtttc
    61261 gagcgggaat ttcatcggtg ctgctggcgt ggactcctct ggcaatggtt gttctggcgt
    61321 catctgttta accatcatct ctgcaagccc cagacctttg cccgccgtca tctgttgggc
    61381 aatctgctgg tcatacatac tggtatacag gcgagtgtgc tcgctgctga acaggccatc
    61441 ttttggtaaa gcgtcgcgca tgcttttcaa catcatctgc acgaacatcc cttccacctg
    61501 acgggccacc ggacggatat ttgccgccgg atcttcgccc gctttcgcct ttagttcgtt
    61561 gagtgattgc gcatcccagg ccgcacttgc cagtagtttg ctgtcgctga tcatcagatg
    61621 atttccagtt ttgcccgcag acatcccgca ctttgcattg attgcagtat ggacatcaga
    61681 tccatcggcg tagcgcccag cgcattgagc gcgcgcacca cgttattgag gctggcgctg
    61741 gaacgtacgc tttgcagcga accgccgctc tggcgtaaat cgatctgcgt ttgtggagta
    61801 accacagtct gtccaccacc aaacggtgta tctggctggc tgacattggc ctgacgattg
    61861 actgttactg agagatttcc ctgcgctacc gcgcagctgt cgagggtcac ttcgcgattc
    61921 atcaccaccg aaccggtgcg cgagttaatc actactttag cgtcctgcgg ggtgacatta
    61981 acctgcatat tctggatatc tgcaaggaaa cggacctggg aactgttgcc acttggtacg
    62041 cgcacctgaa tagtccgcgc atctaacgcg gtggcgctgc catatccacg cacgcggttg
    62101 atggtgtcag cgatttgctg cgccatgctg aaatcttcgt cgttaagttg caaattaagg
    62161 gtattcccga cgccaaactg gctgggcaat tcacgttcaa taaccgcacc attggtgatc
    62221 cgtccaccgt tcagttggtt cacctgaaca ctgctaccgc cagcggaggc tcctgcgccg
    62281 ccaaccagaa tattgccctg cgccagcgca tacacctgac tgtcaacgcc cttaagcggc
    62341 gtcatcaaca acgtacctcc acgcaagctt ttggcatttc ccatggaaga aaccaccaca
    62401 tcgatggttt gcccctgacg tccaaacgga ggaagtgact ctgtcaccat taccgcagcg
    62461 acgtttttta gctgcatatt ggtgcccgtc ggaacggtaa ttcccagctg tgagagcatg
    62521 ttattaagcg tttgtgtggt aaacggcgtc tgggttgtct ggtcaccggt gccatccagc
    62581 cccaccacca gaccatagcc aatcagtgag ttttgcctta ccccctgaac actggtgaga
    62641 tcgcgaatac gctcagcctg agccgccgtc gtaaccagta gaagaattaa tgcagagaga
    62701 aatttaatca ccacagcctc acttacattg gcgacaggtt aaggaagaaa cgctgcaacc
    62761 agcccatatt ttgcgcttcg ttaatgtagc cattgcctac gtattcaatg cgcgcatccg
    62821 ccacctgagt agacggtacg gtattgctgc cgctgatagt gcgtggatta accacgccag
    62881 agaagcgaat aaattcggta ccctgattaa tggcaatctg tttttcaccc accacatgca
    62941 ggttgccgtt gaccagtacc tggtcaaccg tcaccgtcaa cgtgccgcta aaggtattgc
    63001 tggcattggc cccgcccttt ccgttgaacg tgttaccacc ggaggcttcg acatcggcac
    63061 gagcgttacc aaacagcccc tgcaaatagc gcggcacagt atcaaagcca aaattagttt
    63121 taccgtcacg gctggcattc gcagaggagc ttttgctggc gctgacgttc tcctgcaaca
    63181 cgatggtcag cgtatcgcca atattgcgtg gtcgacgatc ttcaaacagc ggttgatagc
    63241 catagttaat cggctgagca gactggaaaa tagaaccgtt ggcgacgggc gtcggaccgg
    63301 gaaccggttg tgcactggtc gccccctgca ccagcggcgt ggagggtatc caggcgcagc
    63361 cggttagtga aagcaccaac aagctggaaa tggcataagt atgcgcagcg tttttttgca
    63421 tggctatcat cttcaaaaat cagtaaaccg gtgaacctgc caccggttaa gccttagagt
    63481 tgcgtcagtt tttgcagcat ctgatcggtg gtggacaccg ctttactgtt gatttcgtaa
    63541 gcgcgttgca cctgaatcat attgaccagt tcttccgcca cgttgacgtt agacgtttca
    63601 acataccctt gatacagcag tcccgcgccg ttcaggcccg gcgtgctttc gttcggtaca
    63661 ccagaggatt gcgtttcggt gtagaggttt tcgccaatgc tctccagccc ggtgtcattc
    63721 ataaaggtgg tgagattgag ctgcccaacc tgaaccggag ctgcctggcc ttgttgggtt
    63781 acgctgacca cgccatcacg accgatggtg atacttaacg cattcgccgg aatggtgatc
    63841 gctggctgca cctgaaaacc accagctgcc cgttctgatc cacctggaaa gagccgtcac
    63901 gggtataggc tgatgaacca tctggcaaca tcacctggaa aaagccctgc cctttaatcg
    63961 cgacatcttt gctgttgttg gtctgcgaca ggtttccctg gctgtgtaag cgttcagtgg
    64021 cgaccgggcg tacccccgtg ccgatttgta atccggaggg taaggtggtt tgttcggaag
    64081 actgtgcccc cggctggcga atggtttgat aaagcagatc ttcaaacacc gcgcgctgac
    64141 gcttaaaacc gttagtactg acgtttgcca ggttgttggc aatgacgtcc atattggttt
    64201 gctgggcgtc aaggcccgtt ttggcgatcc ataatgaact gatcataaaa tgtcctgtag
    64261 tggattagtg acaaagtgca tatgtcttct tgccggatgc ggcgtaaacg ccttatccgg
    64321 cctacaagtg ctcgcaaaat cgataaattg cgattgcccc catagacccg ataaacttgc
    64381 gcatcgggca acttatactt gtcatgtatc ctttcaatta actcatcgac agcagttggt
    64441 tggcacggcc tgcgttatca tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc
    64501 gcgcgctggc aatcatgtcg ctcattgccg caacggcatt gacgttactg ccttccagaa
    64561 cccccgacat cacacgcaag gttggatctg cctgcagtac cggccctcgc gtggcctggg
    64621 tttctgcgct taaacgaaaa atgccgtcgt caccgcgctg cacttcgctg cccgtggctt
    64681 tcaccagttt cagacgccct actggcgcaa ctgtatttgc cggatcgccc ggattgagcg
    64741 ccgagattgt gccatcggca gcaatagtga tttccgctcc ttccggcaca gcaattggcc
    64801 ccgcctcgcc tatcacctga tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa
    64861 tgctgccatt acgcgtatac ccttcgctgc cgtcagcggt ctgcacggcc agccagccat
    64921 cctgctgcaa cgcgacgtcc agcgggcgcg aggtgtaatc cattttgccg ggcgtcatat
    64981 ctgcgcccgg cgttgacgcc gtgaccaacg tgcgcgtggg cagagaaagc ccttccactg
    65041 gcaccgcgcg taaagcattc aactgcgcgc gaaaaccggg cgttgaggca ttggccagat
    65101 tactggcggt taccgcctgt tgattcagtg tctggctggc tgctcccatc gcggtataaa
    65161 ttgcgtgatc cattgagcta tcccgtcagc gattagcgta agttaaccag cgtgttgagg
    65221 atctggtcct gagttttgat ggtctgggcg ttagactgat agttacgctg ggcaacgatc
    65281 atattgacca gttctttact gagatcgaca ttggacgctt ccaacgcacc gttggtcagg
    65341 gtgccaaagt tgcccgtccc ggctgtcccc aacagcgcca cgccagaaga ttgcgtcgca
    65401 gaccagacgt tgtcgccttc ggatgccaga ccttcgttgt tggcaaagtt cgccagtaca
    65461 atctgcccca gcagttgtgt ttgttcgttg gaatagttgc cgacaaccgt accgtcatca
    65521 ttgatttgat aactcaccag atcgcccggt ttgtagccat tctgggtggt tgccacaata
    65581 ttgttagcgc cggtattttg ctgcatggag ttgaggaagc tcaggctaaa cgtggcggga
    65641 tctgcgccgt taattgcgcc agtggtaata ttctctgttg gatttgaggt cagaacgcca
    65701 ttggcattaa acaccagctt cattgcaggc tcggctgtac ctgttggatc actgctatcc
    65761 tgggtgtaga cctgccagtt attatccccg gtcttcacaa agtagacgct catgtcatga
    65821 gcattaccct gactgtcgaa aacagtcacc gaaccttttt tgttatagct atccgcattg
    65881 ctggcgctga atggcgtaac agtaggaagc ggatcactgg aattcaggtt gatctgcatc
    65941 gacgccgtgg tggtagtttt cgctgccatc agggtattcg ggatcgaaat attggtcgga
    66001 ttcgcccctt gctgaatagt cggcggcgta ccggttgccg ggtaacccgt cagctgtaaa
    66061 ccttgcatat tcaccaggtt acggttttca tccagcttaa attgtccgtt acggctgtag
    66121 aacaccgaac cgttgctgtc taccagacgg aaaaaaccgt tctggctgat agcaacgtcc
    66181 agacctcgcc cggtgttggt ggtcgtgcca tcggtaaagt cctgagtgat accggcaact
    66241 tttaccccca gtcccacttt cgaaccggca aacatatcgg caaaagaggc cgtgcctgat
    66301 ttaaagccgt aggtggcgga gttggcgata ttgttgccaa taacatcgag gttggtggcg
    66361 gcagcgttta atccgctaac cgcttgagaa aaggccatga ctgactcctg aaatgtgaag
    66421 gcttaaatta tctgccgtac ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc
    66481 gtattaccgc tgttgccgcg gagcacaccc tgcaccagag caaactgcag cggctgggca
    66541 accagttgtg taccaccgtt actggcgcta atcgctacat tgtaagaacc gttcggcgca
    66601 gtgctgccat cagtcaacgt accgtcccag gtgaaactgt ggactccggc ggtcagttca
    66661 ccaatatcaa tggtgcgcac aaccgcgcca tttttatcgg tgatggtggc cgtaactttg
    66721 tctgccgcct gttgcagctc aacaccaaac ggcgtggtcg tggtcacagc cccttcttca
    66781 ctgccggttc ccgcaagaac agtggtgccg gggatcatca cgccgtgacc gatcaggtta
    66841 ctggcctgta acgactggct gttatcaatc tgtccggaaa tagatccgag cgtggtattg
    66901 agtttttcaa tcccgctgac cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt
    66961 tccattggat tggtcgggtc ctggtttttc agctgcgcca ccaacaaagt cagaaaactg
    67021 ctttgtaagt ctgcggcgtt gctgcccgtg agcgaactac tgctggtggt actgacgccg
    67081 gtatttgtcg gatcggtggt ggttaccgca atggacatag ctttctcctt tattgaccga
    67141 gcgtaagggt tttcagcatc atgcttttca ccgtgttgag cacttcaaca ttggcctgat
    67201 agctgcgtga cgccgacatg gtgttaacca tctccccgac gacatcaacg ttcggcattt
    67261 ttacgtagcc ctttgcatcc gccagcggat tacccggttc ataaaccagt ttgtccgggg
    67321 cctgactttc tataacatcg gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt
    67381 taacctggaa taccacctgt tttgcccgat atggctgtcc atcgggaccg gtcacgctat
    67441 cagcattcgc cagattactg gccgccacgt tcaagcgctg ggactgggca gttaacgccg
    67501 acccggcgat atcaaaaata ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt
    67561 tcatcatgcc tttgatttgc ccgctcaacg cgctaaggct catctggtat tgcaggctgt
    67621 tatcggcaaa ctgggtgcgt tcgcgatcca tatcgacggt attaccgtca agcgaaggct
    67681 ggtccggaat acggtattgc agttctgcgg taggaggcgt cagcgcctgc gccggaatgt
    67741 gttgcgttga ggtcatcgtc agtgcaacca cactggttgc atcccgtcca cgttgcatga
    67801 cttttttaag ttcactggca aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat
    67861 tggcgatgtt tgctgccagc acttcctgac gctgggcgcg cagattgagc gcctcttgtt
    67921 gaaaacgtaa ggcggcgtcg agcttatcga gcatatctcc tccgcaggta tcaaaattct
    67981 gccatcagct taaatgcctt tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg
    68041 ctatttatgc cgttgatgac cattgcggac aggtacaatt cacgttgtag aaatggctgg
    68101 gggcgaaaat gctggcaata aaacgtagcg tggcgatcat ctcgatacta ttcactccgt
    68161 taagtgcggc gagtaatctc acatcgcaat tgcaaacctt ttttagcgcc cagctcgcgg
    68221 gggtaagtga tgaggttcgt gtttctattc gtacagcgcc caatctacta ccgccgtgcg
    68281 agcagccatt gctttcgatg agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg
    68341 cacgctgcgg taacgacaaa cgatatttac aggttaatgt acaggccaca ggaaattatg
    68401 tggttgccgc gatgcccatt gtgcggggag gaaagctgga agctggcaat gtcaaactaa
    68461 aacgcggacg gcaggatacc ctgccaccgc gtacggtgct ggatatcaat caacttgttg
    68521 atgccgttag cctgcgcgat ctatcacccg atcaacctat ccagttaacc cagtttcgcc
    68581 aggcatggcg ggtaaaagcg ggacaacgcg tcaacgtgat cgccagcggt gacgggttta
    68641 gcgccaacgc agaaggtcag gcgctgaaca atgcagccgt cacacagaat gcgcgggtgc
    68701 gcatggtatc gggactggtg gtcagcggcg ttgttgatgc agatgggaat attcttataa
    68761 accagtaagc tgttaaagat tacccgtccc ttgccgataa ataagcaaca catgataaaa
    68821 gcgccctcaa tgaggaataa accatgagta ttgatcgcac ttcgcctctg aagcccgtaa
    68881 gcaccgttca accacgcgaa accactgacg cgtcggtaac gaacacccgg gcggcaaaaa
    68941 caacagcctc caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac
    69001 ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg
    69061 gtgaactaaa aatggacacc ggcaaaattg ccgatgcgct gctcaacgaa gcgcagcaag
    69121 acttgcagag taactgatcg tatgacacgt cttgcagaga tcctcgacca gatgtccgct
    69181 gtgcttaacg atctcaaaac ggtaatggat caagaacagc aacatctctc tatggggcag
    69241 atcaacggca gccagttgca atggattaca gaacaaaaaa gctcactgct ggcgacgctg
    69301 gattacctcg aacagttacg caggaaagaa cccaatacag caaatagcgt tgatattagt
    69361 caacgctggc aggaaattac tgtgaaaacg cagcaactac gccaaatgaa tcaacataac
    69421 ggctggttac tggaaggaca gattgagcgc aatcaacagg cgctggaaat gttgaaaccg
    69481 catcaggaac cgacgctata tggggcgaac ggtcagacct caacaaccca tcgcggcggt
    69541 aaaaagattt cgatctgaat attatctccg gcctgcactg caggccggaa tgcattgtta
    69601 caccgtccgg cgggcaaatt ctttaacttt gaagcccagt accgccagtg cagcgaagta
    69661 cgcggcaatc cccgccagca cgaccgccat taaacgcagt aagcgccagg gcatggtacc
    69721 caatgaccac tccggcatga tatgtaacat acctaaaagc acgccagaca tcaccagtac
    69781 cgccaccacc agacgcaaca gaaacgccat ccagccgggt tgcggagtaa agattttctg
    69841 cttacgcaac tgccagtaaa gcagcgaagc attcagacac gccgccagac caatagaaag
    69901 tgacagccca gcatgtttca acggaccaat aaacgccagg ttcatcaatt gcgttaaaat
    69961 cagcgtaacg atggcaattt tcactggcgt tttaatgtcc tggcgggaat aaaagccagg
    70021 agccaacact ttcactacaa tcaggccaat caaacccacc gagtaggcaa ttaacgcccg
    70081 ctgggtcatc agcgcatcaa acgcggtaaa tttaccgtac tggaacagcg aaacggttaa
    70141 cggacccgaa agaatgccca acgcaaccgc actcggcagc gccaacagga aacaaagacg
    70201 caacccccag tccatcaaac ggttgtattc atcatgattg ccactggcaa aacttttcga
    70261 cagcgacggc agcaaaatgg tgccaagcgc cacgcccagc acaccggagg gaaactccat
    70321 taagcggtcg gcgtaataca tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat
    70381 ggtgttgatg attaaggaga tctggctcac agagacgcca aggatcgccg gtcccatctg
    70441 tttcaccacg cgcattgctc cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc
    70501 gatcttcttc aggtgcggta gctgatacac cagctgcagg atgccgccga ccgtaacagc
    70561 ccacgccagc gccagcaccg gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat
    70621 catgctgatg ttaagcagtg ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt
    70681 cagaatcgct cccaccagcg acgccaggga gatcagcaag atatagggaa aggtaatctt
    70741 tagtagctgg ctggtcaggg caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac
    70801 catgatcacc cacggtgcgg cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt
    70861 cagcaggcca gaaacataag agacaaagac ccgcgtggcg tcttcaccct gcttactttt
    70921 atattccgcc agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg
    70981 taacaagtta ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa
    71041 gattctggcg acaattgcgt ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga
    71101 gctgacggcg gccagcgatt ttaataaatt catcggtgtt ctaatccaga cccacgggca
    71161 aaacgcctgc aaggcaggcg ctgaaagagg cgaatagtct acctggatta tggtgaattg
    71221 ctaccgccag atgttacagg gtttattcac tcatcgcatc gcgccagatc ttgtcaacga
    71281 tacgttgcgc cagcactgcc tgttcgccgg cggtttgcag aactgtctga ttttgcacac
    71341 attcaatgaa gtgccgcgca cagccgacaa atccacgttg ctcaagagtg ctctgccaac
    71401 caggaatcgg tttatgcact acgccctgcc cgcgctcctc acgccattcg cgcatatccg
    71461 taatgtcgat gagcgcatcg tcagtcacgg cctgcacggt ttcacgctga cttccggcac
    71521 ggcgatgcat acaggtggtg atctgcaaag gatcggccga aaaatggtgc tcggcaaaca
    71581 gcatttcgcc agcgtcgtta gtcagtagcg taccgccatc cagagaggct ttgccgcccg
    71641 acaaccacag cgcggtatcc accacatgca gataatcatc cagcaacgtg aaataaagat
    71701 cgtgtggccc gacactattg ctacgatgtt tatccattct tagcgaagct gcggtagcga
    71761 gttgcgtttt taactcaccg tagagtggtg cgaaacgacg gttaaaaccg accatcaacg
    71821 tcagtttttt ccgcgccgcc agttccacca gccgttcagc atcgcgcaga ttttctgcca
    71881 gcggtttatc gacacagaca tgtacacccg cattgagtaa cgtactgacc acgtcaaagt
    71941 ggctggcggt gctggaatgc acaaaaaccg catcgcaact ggcggcaagg ctggataacg
    72001 aatcggcata aggaatgcgc cagctttcac aaattggcag ggctttcgcg cgcgtaggcg
    72061 accaggctcc ttgtaacatc cagtcagacg ctgccgccag caccggtaac cacgcttttt
    72121 gcgcaatgcc gcctaatccc acaacgccga tacgtaattt tttcacttta atctcccaga
    72181 tgggccagca acgaatcaag acgctgtttc agttctgcca cttcgatttc cagggcttcg
    72241 acgcgggcct gtaaatcacc atcaaccgcg ttcgacatat ccgttaccgc cggctgatcc
    72301 tctacctcac cactgaaaag atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc
    72361 agacgcacca caaaaggacc atcttcgcga tttgccagtt gttccagcgt cgactccact
    72421 tccgccatat cgctgaattc atacattcgc gcggcgcggc tgcgcagttc acccggcgtc
    72481 tgggcaccac gcaataacaa cgtggtgatt aacgccactt ccgctgcgct cagtttcaga
    72541 tcgccaaatt ctgaattaca aaaacgttgt tcatatttgg tgacccgatt accaaagcca
    72601 ctcaccgtgc gcagataatg gcgtttgacc agattatcca gttgctcctg cacttcagat
    72661 tcactcagat tcatgaccgg ttcacggttc gttttctgat tacaggccgt gactacacca
    72721 ttgactgaga gcgggtattg ctccggcgtg gtcacctgct tttccagcaa gcaaccaatc
    72781 acgcgagctt caagggcagt aagttgatat ttcatgagat tctccttagc ggccgggcgt
    72841 ccagtctggg gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat caatcaacag
    72901 atagtctttc gcatagcctt ctttttcaaa acccagtcgc gccagtaaat caccgctgcg
    72961 tttattgtgc ggcatataat tagccataat gcgatgaata tgttgggtgc gctgcatata
    73021 acgaatggct gcggtcaggg cttcaaacat gagtcctttg ccctgccatt tttgcccaat
    73081 cgaataaccg agatagcagg catgaaaaga gccacgaaca acattggaaa aattggcaac
    73141 gccaataatc tctttttcgt ccgggtcgaa taagccaaag tagaaagctg aaccttgttt
    73201 atgaaattcg ttaatcatcc ccagcctggc ctgccagcct gatggataac agtggctttc
    73261 gtcgcgcact ggctcccagg gcttgaggaa atggcgattc tctgcgtaat aatccgcaag
    73321 acgccaggca tcacgatcat gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac
    73381 ttttggcacg ttactgcgat agccaaacat tctataccta ctccttcccg taacgtcttt
    73441 aatacgctgt aagtattact atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc
    73501 attttctgca ttattcacat atttgataaa aactctctat caaagcgacg ttttgataaa
    73561 aaaatattgt ccccgaagtg atgggaaaag tcttagcggc aatgcagaat agaaagtgct
    73621 catcttttat ttcccgggag gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa
    73681 atatttcctg ctcatcgata atatgctggt cgtgctgggg ttctttgttg tcttcccgct
    73741 gatctctatc cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg gtattgctct
    73801 cggtctacgc cagtttattc agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg
    73861 ctttggtgcc aaaccgatga ttgttaccgg tatgctgatg cgcgccgccg gattcgccac
    73921 aatgggtatc gcccacgaac cgtggctatt gtggttttca tgcctgctct cgggactcgg
    73981 cggcacgttg tttgatccgc cgcgttcggc gctggtggtg aaactaatcc gtccacaaca
    74041 acgttgtcgt tttttctcgc tgttgatgat gcaggacagt gccggtgcgg tcattggcgc
    74101 attgttgggg agctggctgt tgcaatacga ctttcgcctg gtctgcgcca caggggccgt
    74161 tttgtttgtg ctgtgtgcgg cgttcaatgc gtggttgtta ccggcatgga aactctccac
    74221 cgtacgcacg cccgttcgcg aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac
    74281 ctatgtcctg acgctggcgg gttactacat gctggctgta caagtgatgc tgatgctgcc
    74341 aattatggtc aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt atgccattga
    74401 agcgtgtctg tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa agcattttcg
    74461 tctggaacac cggttgatgg ctgggctgtt gataatgtca ttaagcatga tgccggtggg
    74521 catggtcagc ggtctgcaac aacttttcac cctgatttgt ctgttttata tcgggtcgat
    74581 cattgccgag cctgcgcgtg aaaccttaag tgctttgctg gcggacgcaa gagctcgcgg
    74641 cagctatatg gggtttagcc gtctgggtct ggcgattggc ggcgctattg gttatatcgg
    74701 tggtggctgg ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat
    74761 gctgggaatt attggcatct tcactttcct tgcgctgggt tggcagttta gtcagaaacg
    74821 cgcaacgcgt cgtttgctgg aacgcgacgc ctgatttgat actcccccct gactcctcca
    74881 tactgaaagt gaactgacag ccaaatggag gagtcacgtg aagctataca tttacgatca
    74941 ctgcccttac tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt
    75001 acatgttctg ctcaacgacg acgcagaaac acccacccgg atggtcggtc aaaaacaggt
    75061 tcccattctg caaaaagatg acagccgcta tatgccagaa agcatggata tcgttcacta
    75121 tgtcgataaa ctcgacggca aaccgttact gaccggcaaa cgttcccctg ccattgaaga
    75181 gtggctgcgc aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc
    75241 ggcatttgat gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc
    75301 cagcgcgggt aattttgccg acctgctggc ccactctgac ggtctgatta agaatatcag
    75361 cgatgatttg cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga atggcgaact
    75421 ttcggaagat gatattcagc tatttccgct actgcgtaat ctgacgctgg tagccggaat
    75481 caactggcca agccgcgttg ctgattaccg cgataatatg gcgaaacaga cacaaatcaa
    75541 tttgttatca tcaatggcga tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt
    75601 gccgccgttt tcagtttttt ctgctggcga gtttatcgct ggtgacacat gctgcgttgt
    75661 gtcaggatat tgacgcagat ttcatgtcga ggactaccat gaacaagttt ttatttgccg
    75721 ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca actcacccaa tacaccatca
    75781 ccgaacaaga aattaaccag tcgcttgcga aacataataa tttctcaaaa gatatcggtt
    75841 tacccggcgt ggctgacgcc catattgttc tgacaaactt gatcagccaa attggtcgcg
    75901 aagagccgaa taaggttacc ctaaccggag acgccaatct ggacatgaac tccctgttcg
    75961 gtagtcagaa agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa
    76021 aaggtgcgat cttcctgaaa gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa
    76081 tgcaaacggt gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta
    76141 accagcaacc tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa
    76201 aactggcgaa aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat
    76261 gacgagggcg cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct
    76321 ttgtgtccgg caaaaacatt ccttcagccg gagcatagag attaatgact gcaccatccc
    76381 aggtattaaa gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt
    76441 taaaaactgt cgtgccgtat accagcgaaa tttatggacg ggctatcgta atgcccaatc
    76501 tggctccgcc cgtgaccaca gttgaggctg ccgtggcgta tcgccaacgt attcttgacg
    76561 ccgtacctgc cgggcacgat ttcaccccat tgatgacctg ttatttaaca gattcgctgg
    76621 atcctaatga gctggagcgc ggatttaatg aaggcgtgtt caccgctgca aaactttacc
    76681 cggcaaacgc aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg
    76741 tacttgagcg catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg
    76801 cagatatcga catttttgat cgtgaagcgc gctttataga aagcgtgatg gaacctctgc
    76861 gccagcgcct gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg
    76921 actatgtccg tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt
    76981 ttaaccgcaa ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc
    77041 tcaaacgcaa tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag
    77101 tattcctcgg tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct
    77161 gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga
    77221 tgaatgctct gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatgggt
    77281 tgccggtcaa cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca
    77341 tcgcactgac tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg
    77401 ttaaacaata aaaatcgtag cttcctgttg tcattaggtt atttaacctg tataaataac
    77461 cagtatattc aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca
    77521 ttgccagctg gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg
    77581 tttcctgata atgaaggcca cgtatcggta cgttatgccg cagcgaataa tttatcggtt
    77641 attggcgcaa caaaagaaga taaacagcgc attagcgaaa ttctccagga aacgtgggaa
    77701 agcgccgatg actggtttgt cagcgaataa tatgcagtga tttttgtttt tgccgggtcg
    77761 ccccggcttt tttattttac ttacgaattt tgagtacaaa atttctacac ttcctcaaaa
    77821 taattgcaca atatgctaaa aaaatatgaa tgacatgctg ttttattgtt catataaatc
    77881 ttaaaagcag aaatttatct gttcccccct ttaaaaattc ggtgaatacc cttacttatt
    77941 ggtatactga agttgcttca gataaaaacg cattgaacct cgaataacgt tgtctagtaa
    78001 cacgaattag ggggccatga tggaaaaaaa taatgaagtc attcagactc atccgctcgt
    78061 agggtgggac atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt tgcactacca
    78121 gaccccaaat aagtccgagc aggaagggac tgaagttggt caaacgctct ggttaaccac
    78181 tgatgttgcc agacaattta tttcgatatt agaagcagga atcgccaaaa ttgaatccgg
    78241 tgatttccag gtaaacgagt atcggcgtca ttaacatgac atgaccatcc acccaaaagg
    78301 cacctcaccg gtgcctttac catttctggg ttgtatatct gcattcagtt aattactctg
    78361 cttaaaccat gagagcagag agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg
    78421 ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac
    78481 cgacgcccat atgccaccgc atcaacacgg cagccaccac ggcgatacgc gattaattcg
    78541 ccatgcttat ggtgaaggcg aaaagtatgt cccgctggtc ctccgcgcgc aaacgctgtg
    78601 ggatgaactt tcccgccaca acgaagatga tcccattttt gtacgctctg gtgtcattaa
    78661 cctcggcccg gctgactccg catttctcgc caacgtcgcc cacagcgccg aacagtggca
    78721 actcaacgtt gaaaagctcg atgcgcaagg gattatggcc cgctggccag aaatacgcgt
    78781 cccggacaac tacatcggct tatttgagac tgattccggt tttttgcgca gcgaactggc
    78841 gattaaaacc tggatccaac tggcgaagga agcgggctgt gcgcaactgt tcaactgccc
    78901 ggtcaccgca attcgtcatg acgatgatgg cgtaactatt gaaacggttg acggtgagta
    78961 tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta aaagacctgc tcccggagct
    79021 gcctgtccag cccgtacgta aagtatttgc ctggtatcag gccgatggcc gctatagcgt
    79081 gaagaataaa ttcccggcgt ttaccggtga actgcccaat ggcgatcaat attatggttt
    79141 tccggcagaa aacgacgcgt tgaagattgg caaacataac ggaggccagg ttatccattc
    79201 agcggatgaa cgtgttccgt ttgcggaagt ggtcagcgat ggttcggaag ccttcccgtt
    79261 cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct gcacctatga
    79321 taattcgcct gacgaagatt ttattatcga taccctaccc ggccacgata atacactgct
    79381 cattaccggc ctgagtgggc acggttttaa atttgcgtca gttttagggg aaatagctgc
    79441 cgattttgcg caagacaaaa aaagcgattt tgatttgacg ccattcaggc tttcccgctt
    79501 ccaataatca tcattacggc ctccagaatg aggccgtttt tacaggagta tttaatgcgt
    79561 cggctgttgc actatctc
//
LOCUS       DAETQN010000004        74366 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.004, whole genome shotgun sequence.
ACCESSION   DAETQN010000004 DAETQN010000000
VERSION     DAETQN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 74366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.004"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            71..1015
                     /locus_tag="K0W68_000294"
                     /pseudo
     CDS             71..1015
                     /locus_tag="K0W68_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="K0W68_000295"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="K0W68_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HBI3071976.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="K0W68_000296"
     CDS             1797..1904
                     /locus_tag="K0W68_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBI3071977.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="K0W68_000297"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="K0W68_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBI3071978.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="K0W68_000298"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="K0W68_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBI3071979.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4696)
                     /gene="yehY"
                     /locus_tag="K0W68_000299"
                     /pseudo
     CDS             complement(3540..4696)
                     /gene="yehY"
                     /locus_tag="K0W68_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(4703..5620)
                     /gene="osmF"
                     /locus_tag="K0W68_000300"
     CDS             complement(4703..5620)
                     /gene="osmF"
                     /locus_tag="K0W68_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBI3071980.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5872..8169)
                     /gene="bglX"
                     /locus_tag="K0W68_000301"
     CDS             complement(5872..8169)
                     /gene="bglX"
                     /locus_tag="K0W68_000301"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBI3071981.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8365..10080
                     /gene="dld"
                     /locus_tag="K0W68_000302"
     CDS             8365..10080
                     /gene="dld"
                     /locus_tag="K0W68_000302"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBI3071982.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10119..11051)
                     /gene="pbpG"
                     /locus_tag="K0W68_000303"
     CDS             complement(10119..11051)
                     /gene="pbpG"
                     /locus_tag="K0W68_000303"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBI3071983.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMLVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11225..11812)
                     /locus_tag="K0W68_000304"
     CDS             complement(11225..11812)
                     /locus_tag="K0W68_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBI3071984.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11982..12560
                     /locus_tag="K0W68_000305"
     CDS             11982..12560
                     /locus_tag="K0W68_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3071985.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12690..13451)
                     /locus_tag="K0W68_000306"
     CDS             complement(12690..13451)
                     /locus_tag="K0W68_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI3071986.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13504..14940)
                     /gene="mdtQ"
                     /locus_tag="K0W68_000307"
     CDS             complement(13504..14940)
                     /gene="mdtQ"
                     /locus_tag="K0W68_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBI3071987.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15164..15247
                     /gene="yohP"
                     /locus_tag="K0W68_000308"
     CDS             15164..15247
                     /gene="yohP"
                     /locus_tag="K0W68_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBI3071988.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(15620..16567)
                     /gene="dusC"
                     /locus_tag="K0W68_000309"
     CDS             complement(15620..16567)
                     /gene="dusC"
                     /locus_tag="K0W68_000309"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBI3071989.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            16806..17204
                     /locus_tag="K0W68_000310"
     CDS             16806..17204
                     /locus_tag="K0W68_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBI3071990.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            17201..17896
                     /locus_tag="K0W68_000311"
     CDS             17201..17896
                     /locus_tag="K0W68_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBI3071991.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            18026..18910
                     /gene="cdd"
                     /locus_tag="K0W68_000312"
     CDS             18026..18910
                     /gene="cdd"
                     /locus_tag="K0W68_000312"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBI3071992.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            19060..19779
                     /gene="sanA"
                     /locus_tag="K0W68_000313"
     CDS             19060..19779
                     /gene="sanA"
                     /locus_tag="K0W68_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBI3071993.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            19782..20021
                     /locus_tag="K0W68_000314"
     CDS             19782..20021
                     /locus_tag="K0W68_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBI3071994.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            20215..21453
                     /gene="preT"
                     /locus_tag="K0W68_000315"
                     /pseudo
     CDS             20215..21453
                     /gene="preT"
                     /locus_tag="K0W68_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            21447..22682
                     /gene="preA"
                     /locus_tag="K0W68_000316"
     CDS             21447..22682
                     /gene="preA"
                     /locus_tag="K0W68_000316"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HBI3071995.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(22809..23819)
                     /gene="mglC"
                     /locus_tag="K0W68_000317"
     CDS             complement(22809..23819)
                     /gene="mglC"
                     /locus_tag="K0W68_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBI3071996.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(23835..25355)
                     /gene="mglA"
                     /locus_tag="K0W68_000318"
     CDS             complement(23835..25355)
                     /gene="mglA"
                     /locus_tag="K0W68_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBI3071997.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(25416..26414)
                     /gene="mglB"
                     /locus_tag="K0W68_000319"
     CDS             complement(25416..26414)
                     /gene="mglB"
                     /locus_tag="K0W68_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBI3071998.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(26694..27734)
                     /gene="galS"
                     /locus_tag="K0W68_000320"
                     /pseudo
     CDS             complement(26694..27734)
                     /gene="galS"
                     /locus_tag="K0W68_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            complement(27876..29033)
                     /locus_tag="K0W68_000321"
     CDS             complement(27876..29033)
                     /locus_tag="K0W68_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBI3071999.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(29050..29718)
                     /gene="folE"
                     /locus_tag="K0W68_000322"
     CDS             complement(29050..29718)
                     /gene="folE"
                     /locus_tag="K0W68_000322"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBI3072000.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            29976..30812
                     /gene="yeiG"
                     /locus_tag="K0W68_000323"
     CDS             29976..30812
                     /gene="yeiG"
                     /locus_tag="K0W68_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBI3072001.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(30844..32835)
                     /gene="cirA"
                     /locus_tag="K0W68_000324"
     CDS             complement(30844..32835)
                     /gene="cirA"
                     /locus_tag="K0W68_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBI3072002.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(33127..34596)
                     /gene="lysP"
                     /locus_tag="K0W68_000325"
     CDS             complement(33127..34596)
                     /gene="lysP"
                     /locus_tag="K0W68_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBI3072003.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(34801..35682)
                     /locus_tag="K0W68_000326"
     CDS             complement(34801..35682)
                     /locus_tag="K0W68_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3072004.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            35781..36830
                     /locus_tag="K0W68_000327"
     CDS             35781..36830
                     /locus_tag="K0W68_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBI3072005.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            36904..37761
                     /gene="nfo"
                     /locus_tag="K0W68_000328"
     CDS             36904..37761
                     /gene="nfo"
                     /locus_tag="K0W68_000328"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBI3072006.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            37765..38853
                     /locus_tag="K0W68_000329"
     CDS             37765..38853
                     /locus_tag="K0W68_000329"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBI3072007.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(38909..40159)
                     /locus_tag="K0W68_000330"
     CDS             complement(38909..40159)
                     /locus_tag="K0W68_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBI3072008.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(40259..41200)
                     /gene="rihB"
                     /locus_tag="K0W68_000331"
     CDS             complement(40259..41200)
                     /gene="rihB"
                     /locus_tag="K0W68_000331"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HBI3072009.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            41330..42028
                     /gene="yeiL"
                     /locus_tag="K0W68_000332"
     CDS             41330..42028
                     /gene="yeiL"
                     /locus_tag="K0W68_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HBI3072010.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLLDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(42095..43345)
                     /locus_tag="K0W68_000333"
     CDS             complement(42095..43345)
                     /locus_tag="K0W68_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBI3072011.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(43439..44377)
                     /gene="psuG"
                     /locus_tag="K0W68_000334"
     CDS             complement(43439..44377)
                     /gene="psuG"
                     /locus_tag="K0W68_000334"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBI3072012.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(44365..45306)
                     /locus_tag="K0W68_000335"
     CDS             complement(44365..45306)
                     /locus_tag="K0W68_000335"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBI3072013.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(45764..47455)
                     /gene="fruA"
                     /locus_tag="K0W68_000336"
     CDS             complement(45764..47455)
                     /gene="fruA"
                     /locus_tag="K0W68_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBI3072014.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(47472..48410)
                     /gene="fruK"
                     /locus_tag="K0W68_000337"
     CDS             complement(47472..48410)
                     /gene="fruK"
                     /locus_tag="K0W68_000337"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBI3072015.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(48410..49540)
                     /gene="fruB"
                     /locus_tag="K0W68_000338"
     CDS             complement(48410..49540)
                     /gene="fruB"
                     /locus_tag="K0W68_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBI3072016.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            49908..51089
                     /gene="setB"
                     /locus_tag="K0W68_000339"
     CDS             49908..51089
                     /gene="setB"
                     /locus_tag="K0W68_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBI3072017.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(51086..51340)
                     /locus_tag="K0W68_000340"
     CDS             complement(51086..51340)
                     /locus_tag="K0W68_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBI3072018.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            51495..52067
                     /gene="yeiP"
                     /locus_tag="K0W68_000341"
     CDS             51495..52067
                     /gene="yeiP"
                     /locus_tag="K0W68_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBI3072019.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            52290..53756
                     /locus_tag="K0W68_000342"
     CDS             52290..53756
                     /locus_tag="K0W68_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBI3072020.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            53874..54860
                     /gene="yeiR"
                     /locus_tag="K0W68_000343"
     CDS             53874..54860
                     /gene="yeiR"
                     /locus_tag="K0W68_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBI3072021.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            54899..55612
                     /gene="lpxT"
                     /locus_tag="K0W68_000344"
     CDS             54899..55612
                     /gene="lpxT"
                     /locus_tag="K0W68_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBI3072022.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            56024..56590
                     /gene="mepS"
                     /locus_tag="K0W68_000345"
     CDS             56024..56590
                     /gene="mepS"
                     /locus_tag="K0W68_000345"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBI3072023.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            56771..58327
                     /locus_tag="K0W68_000346"
     CDS             56771..58327
                     /locus_tag="K0W68_000346"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBI3072024.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            58409..60223
                     /locus_tag="K0W68_000347"
     CDS             58409..60223
                     /locus_tag="K0W68_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBI3072025.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            60224..61318
                     /locus_tag="K0W68_000348"
     CDS             60224..61318
                     /locus_tag="K0W68_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBI3072026.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            61318..62343
                     /locus_tag="K0W68_000349"
     CDS             61318..62343
                     /locus_tag="K0W68_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3072027.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            62345..63934
                     /gene="yejF"
                     /locus_tag="K0W68_000350"
     CDS             62345..63934
                     /gene="yejF"
                     /locus_tag="K0W68_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBI3072028.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(63938..64282)
                     /locus_tag="K0W68_000351"
     CDS             complement(63938..64282)
                     /locus_tag="K0W68_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072029.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(64615..65735)
                     /locus_tag="K0W68_000352"
                     /pseudo
     CDS             complement(64615..65735)
                     /locus_tag="K0W68_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(65763..66458)
                     /gene="rsuA"
                     /locus_tag="K0W68_000353"
     CDS             complement(65763..66458)
                     /gene="rsuA"
                     /locus_tag="K0W68_000353"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBI3072030.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            66608..68368
                     /locus_tag="K0W68_000354"
     CDS             66608..68368
                     /locus_tag="K0W68_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBI3072031.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            68493..68777
                     /gene="rplY"
                     /locus_tag="K0W68_000355"
     CDS             68493..68777
                     /gene="rplY"
                     /locus_tag="K0W68_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBI3072032.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(68916..69923)
                     /gene="yejK"
                     /locus_tag="K0W68_000356"
     CDS             complement(68916..69923)
                     /gene="yejK"
                     /locus_tag="K0W68_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBI3072033.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            70105..70332
                     /locus_tag="K0W68_000357"
     CDS             70105..70332
                     /locus_tag="K0W68_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBI3072034.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            70352..72112
                     /gene="pbgA"
                     /locus_tag="K0W68_000358"
     CDS             70352..72112
                     /gene="pbgA"
                     /locus_tag="K0W68_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBI3072035.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            72187..72263
                     /locus_tag="K0W68_000359"
     tRNA            72187..72263
                     /locus_tag="K0W68_000359"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72221..72223,aa:Pro,seq:ggg)
     gene            complement(72366..74366)
                     /gene="yejO"
                     /locus_tag="K0W68_000360"
                     /pseudo
     CDS             complement(72366..74366)
                     /gene="yejO"
                     /locus_tag="K0W68_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        18268 a        19590 c        18255 g        18253 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgcagtg
     3781 gtaactgaac atgcaggaat cgctgcgccc cgctcatgcc catcgctctg gcgctctcca
     3841 gcacatcgcg cgggatctgg ttcaaaccga ctaccacgcc gcgcaccagc ggcagcaagg
     3901 catagagcac cagcgcaatc agtgcgggtg tcattccggt tcctgctatg ccgagcgtcc
     3961 ccagccacgg aaaggccgta accagcgcgg caagcggcgc aatcaacagg ccaaagagcg
     4021 ccaccgaagg cacggtctga atgacattga gcagagaaaa aattgccccc tgccgagcag
     4081 tggaaaagta gcaccagatg cccaacggca caccaatcac taacgcaggc agcaccgcac
     4141 caaacagcaa cgtcagatgt tgtgccagcg cgtcgtcaaa cacatcctga cggttggcgt
     4201 attctttcat tagtgagaga tcgttaagcg tgccggagta cagcaaccac agcggaataa
     4261 tggcaatctg catatgcaac aaccagcgcc acagcggatg cgtggagatt cggcggatgg
     4321 catcactaca ggccagcaat gccagcgccg cggccagcca gaaaccactg ccgaggctgg
     4381 tacgcgccag cgcactgcca ttttgcgcca gttgggtcgc cgcctttcct gctccccaca
     4441 ccagcaatac gaagacgaat tgcgccagaa tgagtgcaaa aatgctgcct tttttagcgg
     4501 gaataaaaca ggctgtcagc caggcgcaac caacgcccac cagcatccag atcgtttgcg
     4561 gccacagttg ccagagatga cgcccttcac ccgagactaa acgattaggc gcgtaactga
     4621 taaacggcag cgccgctgcg attgccgtca gcaacagcag cagcgccaga acaggattaa
     4681 tacgtagata agtcacggga aattacttcg tccacccttt ttgtttcagg tagtcggcag
     4741 ccactttttt ggcatccagt ccttccactg caatgctggc attcagttgc tgcaatgttt
     4801 ttgcatcgag gctggcgaag actggctgta gccactgtgc catttgcgga tactctttca
     4861 gcaccgactc acgcaccact ggtgcaggcg cgtagatagg ttgcacgcct ttcggatcgc
     4921 ttaaggtttg cagccccagc gccgcgaccg gaccgtcagt gccgtaagcc attgcggcat
     4981 taacgccaga agtttgctgg gcagcggctt taatcgttac cgccgtgtcg ccgccagcca
     5041 gtgacagtaa ctgatcctga tcgagtttaa agtcgtaggc tttttcaaac gcgggtaacg
     5101 catcggcgcg ttcgataaac tctgccgagg ctgccagttt gaaggtgccg ccctctttca
     5161 gataacgact caggtcggca agcgaagtga gtttgttttt ctctgccaca tcctgacgca
     5221 cggcgatggt ccaggtgtta ttcgctggtg cgggcgtcag ccagattaac ttgttttgct
     5281 ctgcatcgag ttttttgact ttctcgtaac cttgctgcgc gtttttccat gccgcatcgt
     5341 tttcatcttt aaagaagaaa gcgccattac cggtatattc cggatagata tccagttcac
     5401 cggaagtaat cgccccgcgc accacaggag tcgttccgag ttgcacttta ttcaccgtcg
     5461 gtacgccgtg gctttccagc acctgcaaga tgatgttgcc gagtagcgcg ccttcggtat
     5521 cgatttttga accgacttta acgggggaag ccgcctgtag cggcaggctc acggctgcca
     5581 acataaccag tgaacctgcc cagacctttg agagtggcat gatgctttcc tcattctttt
     5641 actgttgttt tcagcgaatt aagagaaaag catagttgat aatgacagag ttagcctgag
     5701 cggaaggatt cagttgcaga atcagataaa tacttaaagg gcatattttg cccggcgttt
     5761 cttgccggat gcggcgtaaa cgccttatcc ggcctacaaa ggtcgaaaat tcagcaagtt
     5821 aagatatgaa catcaggcaa agggcgcaaa cgtcgcgccc ttactaaagc attacagcaa
     5881 ctcaaactcg cctttcttaa cgcgtgcgga atcagtgccg ataaagacat tgaacttgcc
     5941 aggttcggcg tcatatttca tctgttgatt ccagaacttc agcgcctcga tatcaatcgg
     6001 gaagctgaca gtctgagttt cgcccggttt cagggtgatt ttctcaaagc ctttcagctg
     6061 cttcactgga cggctcatgg aagccgtcac atcctgcaag tacatctgta ctaccgtggc
     6121 cccttcgcgt ttaccggtgt tcgtcacctg cacgctggcg gtcactttgc cgtcacgctt
     6181 catggtcggc gcagaaagtt tcacatcaga gacggtgaaa gtggtatagc tcagaccata
     6241 gccgaacgga taaagcgccc cgttagcttc atcaaaataa cgcgaagtgt atttgttcgg
     6301 cttgtcggca ttatacggac gaccggtatt cagatggctg tagtacaccg ggatctgccc
     6361 gacagaacgc gggaaggaca ttggcagctt gccggacggg ttgtaatcgc caaacaatac
     6421 atcggcaatt gcattaccgc cttcagtccc cgcaaaccag gtttccagaa tcgcatcagc
     6481 ctgctgatct tctttcacca gcgccagcgg acgcccgttc atcagcacca gcaccagcgg
     6541 tttaccggtg gctttcagcg ccgtaatcaa gtcacgttgg ctttgcggaa tagtgatatc
     6601 ggtacggctg gaggcctcgt gcgccatccc ctgtgcttca ccgaccacag ccaccaccac
     6661 atcagattgt ttcgccgtct gcaccgcttc atcaatcatc tcttgcggcg agcgcggatc
     6721 gactttgacc gcttcttcat actgattcag gaaatcgata atgcctttgt cactggtaac
     6781 gttcgcccct ttggcataca gcactttacc gttttcaccg acggcatttt taatcccggt
     6841 cagtacggtc acggattgat cggcaacacc ggctgcggac cagctgccca tcacgtcacg
     6901 tttactgtcc gccagtggcc caaccaccgc aatggtggcc gattttttca gcggtaacgt
     6961 ttcgagacgg tttttcagca acaccaggct ttcgcgcgcc acttcacgcg cttctttacg
     7021 gtgcaggcgg ctttcggcat tggtatccac cgggtcagac tctttcggcc ccaaatggct
     7081 gtatgggtcg ttaaacaatc ccatatcata tttaacgttc agtacatggc gggcagcatc
     7141 gtccagctct tccatcgtca ctttgccgga tttgatcaac ccaggcagat acttcgagta
     7201 atactcgtcg ctcatactca tgttgattcc ggatttcagc gccacgcgca ccgcatcttc
     7261 cgggtctgcc gccgtgccat gtttaatcag ctctttgatt gcaccgtgat cggaaacggt
     7321 gatgccttta aagccccact ggtcgcgcag aacatctttc agcagccagg aatcggaggt
     7381 ggctggcgtg ccgttcagcg agttcagcgc caccatcacc gcgccgctgc ctgcgtccag
     7441 ccccgctttg tacggcggca tataatcatt aaacaggcgc tgcggactca tatcgacggt
     7501 gttgtactct ttaccgcctt ctaccgcgcc gtatgcggca aagtgtttga cgctggtcat
     7561 caccgagtag cgatctgccg ggcttttacc ctgcatcgct tccaccatgg ttttacccat
     7621 tgttgaggtg agatacgtat cttcgccaaa accttcggaa gcacgtcccc agcgcggatc
     7681 gcgcgagaca tcgaccatcg gtgcccaggt catattcagg ccatcatctg ccgcttcata
     7741 agcagagaca cgtccgaccg ttttcactgc atcgaggtta aaagacgagg ccagaccgag
     7801 gctaatcggg aacaccgtgc gctgaccgtg cagcacgtcg taagcaaaga aaagaggaat
     7861 tttcaggcgg cttaattcca tcacctgatc ctgcatggcg cggatatcct gacgggttac
     7921 ggtgttgaaa atcgccccaa cctgaccgtc tttgatcatc tcgcggatcg cctctttcgg
     7981 gttatccgga ccgacgctga ttaagcgcag ctgaccaatt ttctcatcaa ctgtcatttt
     8041 cttaagcagt tcggtgacga acgcatcccg cgcttcgggc gttaatggat ggttgccgaa
     8101 taaatcatcc gccagtgctg gctgcagggc cagactcacc gcgattccta ctgaacatag
     8161 ccatttcata tggatttttc ttcctctgtt gcccgacgag gcagcgaaaa tggtgcaaaa
     8221 accgtagttt gccataagca tgatggagag agaaaaagaa tgctcagttt attgtctgaa
     8281 ttttcaaaat attcactcgc tgaattgtca tacaaggcgc tattctagtt tgtgatattt
     8341 tttcgccacc acaaggagtg gaaaatgtct tccatgacaa caactgataa taaagccttt
     8401 ttgaatgaac ttgcccgtct ggtcggtcat tcacacctgc tcaccgatcc cgcaaaaacg
     8461 gcccgctatc gcaagggctt ccgttctggt cagggcgacg cgctggctgt cgttttccct
     8521 ggctcactac tggaattgtg gcgggtgctg aaagcctgtg tcaccgccga caaaattatt
     8581 ctgatgcagg ctgccaatac aggcctgacc gaaggatcga cgccaaacgg taacgactat
     8641 gatcgcgata tcgtgatcat cagcaccctg cgtctcgaca agctgcacgt tcttggcaag
     8701 ggcgaacagg tgctggccta tccgggcacc acgctctatt cgctggaaaa agccctcaaa
     8761 ccgctgggac gcgaaccgca ctcagtgatt ggatcatcgt gtataggcgc atcggtcatc
     8821 ggcggtattt gtaacaactc cggcggctcg ctggtgcaac gtggcccggc gtataccgaa
     8881 atgtcgttat tcgcgcgtat aaatgaagac ggcaaactga cgctggtgaa ccatctgggg
     8941 attgatctgg gcgaaacgcc ggagcagatc cttagcaagc tggatgatga tcgcatcaaa
     9001 gatgacgatg tgcgtcacga tggtcgtcac gcccacgatt atgactatgt ccaccgcgtt
     9061 cgtgatattg aagccgacac gcccgcacgt tataacgccg atcctgatcg gttatttgaa
     9121 tcttctggtt gcgccgggaa gctggcggtc tttgctgtac gtctagatac cttcgaagcg
     9181 gaaaaaaatc agcaggtgtt ttatatcggc accaaccagc cggaagtgct gaccgaaatc
     9241 cgccgtcata ttctggctaa cttcgaaaat ctgccggttg ccggggaata tatacaccgg
     9301 gatatctacg atattgcgga aaaatacggc aaagacacct tcctgatgat tgataagtta
     9361 ggcaccgaca agatgccgtt cttctttaat ctcaagggac gcaccgatgc gatgctggag
     9421 aaagtgaaat tcttccgtcc gcattttact gaccgtgcga tgcaaaaatt cggtcacctg
     9481 ttccccagcc atttaccgcc gcgcatgaaa aactggcgcg ataaatacga gcatcatctg
     9541 ctgttaaaaa tggcgggcga tggcgtgggc gaagccaaat cgtggctggt ggattatttc
     9601 aaacaggccg aaggcgattt ctttgtctgt acgccggagg aaggcagcaa agcgttttta
     9661 caccgtttcg ccgctgcggg cgcagcaatt cgttatcagg cggtgcattc cgatgaagtc
     9721 gaagacattc tggcgttgga tatcgctctg cggcgtaacg acaccgagtg gtatgagcat
     9781 ttaccgccgg agatcgacag ccagctggtg cacaagctct attacggcca ttttatgtgc
     9841 tatgtcttcc atcaggatta catagtgaaa aaaggcgtgg atgtgcatgc gttgaaagaa
     9901 cagatgctgg aactgctaca gcagcgtggc gcgcaatacc ctgccgagca taacgtcggt
     9961 catttgtata aagcaccgga gacgttgcag aagttttatc gcgagaacga tccgaccaac
    10021 agtatgaatc cggggatcgg taaaaccagt aagcggaaaa actggcagga agtggagtaa
    10081 aaattacgga tggcatagta tcgccatccg tcattcactt aatcgttctg tgccgtctgc
    10141 cccgccgccg ccatttgggc ggctttttgt tttttatagc tcaacgctgc tgctggaaca
    10201 agcatcactt taccagtttc aatccaggta cgcaggcggc tggcatcggc aaaatgggta
    10261 tatttgccaa acgcatccat cactaccagc gctaccggtt tattattgat aaccgtacgc
    10321 atcaccagac aatggcccgc cgcattggta aaaccggttt tggttaactg aatattccag
    10381 ttatcgcgat acaccagatg gttagtattg cggaacggca gcgtatacgt cgggttagag
    10441 aaggttgcca tatcttcccg ggtagtactt aactgcccga tcaacggata ttgtttgctg
    10501 gcaatgagca gtttggttaa gtcacgggca gttgaaacgt tatgcaccga caatccggta
    10561 ggttcaacaa agcgcgtgtt gttcattccg agcgatttcg ctttcgcatt cattgcctta
    10621 ataaaggctt tgtaaccacc gggataatgg tgtgcaaggc ttgccgccgc gcggttttct
    10681 gaagacatca gcgccagcaa cagcatatct ttacggctga tttcgctatt cagtcgtacg
    10741 cgcgaataga cccctttcat ctccggcgtc tggctgatat ccacttttag tttttcatcc
    10801 agcggcagtc gtgcatccag cacaaccatc gcggtcatta atttgctgat agacgcaatc
    10861 ggacgcacca gatccgggtg gttcgagtag atcactttgt tggtattcag atcaacaatc
    10921 atcgcgctac cggaggcaat ttcaggttgt gaagcggtgg tagcggctgc cgttttcgca
    10981 acggcctgcg gtgccaaagg cacagccagc atcagggcca ggctaaataa agaaactcga
    11041 aatttcggca tgatgagcat tcagatagtg gttcacgcgc acaggttgcg caccgccgga
    11101 gtaaggattt actgaggcta gcgacgccat cataacgagc aaaaagtgcg atcgtcaaag
    11161 gagaatcgtg aggaaatgct gcattgctga catttacgcc agcaatgcaa cgtcaaagaa
    11221 ctttctagaa caaacgataa ccgtagcccc atagtataac ggttagggcg agcagcactt
    11281 ccagtaccag cacgccaatc gccagcgtcg aactagagaa gctaaggcct tcctctttgt
    11341 tgatattcag gaagctcgga ataccaaggt aaagcaggta gccggtgtaa aacagcgcca
    11401 ccgtgccgac cagcgcacac aaccagacca gtggataaag cgccaccaga ccgcttaaaa
    11461 acagcggagt tgcaacgtag cccgcgaaga ccatgcagtg cgcaagtgac ggacgctgcg
    11521 gataattacg cgccatccac cagatgaccc gccccattac cgcaacccca gccagcataa
    11581 cgccataaaa caggacagcc agcgccagtc cggtaaacca ggataacttc aggatagtgc
    11641 catcgccaaa attccagcca atctgtgtag tgccaatgaa ggcgcaaatc accggaatcg
    11701 ccgccatcag caaaacgtgg tgggtgtaat ggtgagaaat cgtttcgttt tcgcgattaa
    11761 tcacctgcat ttcacgatcg ggatgggaaa acagtcccca gacatggctc ataccgcccc
    11821 cttgttgtga gttcatgaac ctgacagttc aagtataagt cagcttgtga ttattttttg
    11881 ttcgctcatg taatttgcac cgtctgatgt acgtcctttt tcagcgtgaa tgatttccag
    11941 ggtgtatgct tacaggcaac caaagggaga cagactggcc tatggatctc aatacactca
    12001 tctcacaata tggttatgcc gcgctggtga tcggtagcct ggcggaaggt gaaaccgtga
    12061 ctttgctggg aggcgttgcg gcacatcagg ggctattaaa gttcccgctg gtggtacttt
    12121 ctgtggcgct tggcggcatg attggcgacc aggtgcttta tctgtgcggg cggcggtttg
    12181 gcggcaagct gttacgccgt ttctcgaaac atcaggataa aattgagcgg gcgcagaaac
    12241 ttatccaacg ccatccgtat ctgtttgtca ttggtacgcg ctttatgtat ggctttcggg
    12301 tgattggccc gacgctgatt ggtgccagcc agctgccgcc gaaaatcttt ctgccgctga
    12361 atattctcgg cgcatttgcc tgggcgttga tttttaccac tattggttac gctggtggtc
    12421 aggtgattgc gccgtggttg cacaatctcg accagcattt gaagcactgg gtctggttga
    12481 ttctggtcgt ggttctggtg gtgggcgtgc gctggtggct gaaacggcgc gggaagaaaa
    12541 agccggatca tcaggcgtaa aaccattgcc ctgggtaagg cgttcgcgcc gcatccgaca
    12601 tccggtgcac catgcctgat gcgacgcttg tgcgtcttat catgcctacc aaaattttac
    12661 ccattgaaat aaccacgcac ctcactgcgc tattctgggt tgaactgcgg attcgccaac
    12721 ataaagccgc catccactat caacgactgc ccggtggtgt aatttgcgcc ctctgagcac
    12781 agccacgcta ccaggctggc aatctcatgc gttgcgccaa aacgccgcag aggaatcgaa
    12841 ggctccgcgt cgggcttcac gtcgctgtca tccatgccat tcattggcgt ggcgatcgcc
    12901 ccaggcgcga ctgcgttcac caaaatctta tgcctgacca gctccagcgc catcgttttg
    12961 gttaacccgc cgagcgcatg tttagcggct gtgtaagcgc tggcatccgg cagcggcgta
    13021 tgttcatgta ccgacgtaat gttgatgata cgaccgccct gcccttgttt caccatctgg
    13081 cgggccgcaa tttgtgagca taaaaatgca ccatcgacat caacggtaaa aatcttgcgc
    13141 cactcatcga aagccatatc aagaaacggc gctttggtca ttgcgcccgc attattcacc
    13201 agcacatcaa tacgccccag ccgttgaatg agtttctcca gcgcctgcgc accttctggc
    13261 agtttaccga gctccagctg cacgatctcc gcacgtacgc cgtggctaac tacctcacgc
    13321 gcggtatctt ttgccccttc ttcatctgag tgccaggtaa tgccaatatc aaacccctgc
    13381 tgcgccagta ataacgcgca ctctttgccg atccccgaat cggaggcggt aataatcgca
    13441 acctgtgcca tcaagttctc cacttaacgc tgaataaacg ttaagtatag aaggcgcata
    13501 tcatcagcgt ttgtaacccc cgcccaacgc accagtgagt tgaatagaag catccagcca
    13561 ctgcccttgc aataacaggc cattggcccg ctcacgcagc gcggggattc tggcttcgct
    13621 gacgcgggaa ccaacaatga tgcccgcgtt aaagcgcgcc tgcgcaagac ctaccacacg
    13681 caaggcatcg cgctcaattt gcgcctgatg ctggtttttc tccgcaagtg tctgaacctg
    13741 actggctgcc cgcgccacgt cattcaccgc ttcaaccacc gctttgttgt agctggcgat
    13801 agacaagttg ctttcggctt ttgcgatatt gagattggcg ttaagacgac cactatcgaa
    13861 aatgggtagc gtcaggcctg ccgtaacgcc catttgctgc gcggaatgac ggaacagatc
    13921 gcttaagtgc aacgcatcct gttgcaggaa ggccatcagg ttgatgtcag gataaaatgc
    13981 cgcttttgcc gcatcaatgg tgcttagcga tgactcaacg taccagtgcg ccgcctgcaa
    14041 atctgcccgc cgggccagta aggagtaccc cagttcatca ggaagctggc ttgccacttt
    14101 cggcaacgcg accggtttaa gcttcaatga ctttgtctgg ttatttgtaa gtgcgcttaa
    14161 ccgtgcctca ataattttca ttttccccgc gacatcgttg agctgctgcc gggttttgct
    14221 ggcattaata tcggtttcca caccttcaac tgaagaagta atcccgttct gatatagctg
    14281 gcgatcggtc gcgataatgg tgttctgctc tttttctatt tgctgcaaga ccgtgtttaa
    14341 cgccgcctgg gtttgccact cccagtacag gcgggctacg ctgccagcca gcaattggcg
    14401 ggtttgctcg cgttccgccg cccgtgcttt aaccgtaccc aggcgggcag taacctccgc
    14461 ccgattcttt ccccagatat cgagatgcca gcccgccgtt aagccaaaag taccgttggt
    14521 gtaccacggg ccggtcgtac ctgcggccgg atcgttcaga gcaaacggcc ccattaagcc
    14581 ttctgccgac attttttgcc gctccatatc cgccgaaaag tcgatctgcg gaccatcctg
    14641 agtggcaact gccttcgcct gggcttcagc tagctgaatg cgctgttcag ccacctgcat
    14701 atccggtgcg ttctgtagtg cattgttaat taatgaagtg agctgattat cgtgatactc
    14761 cagccaccat tggctgtctg gccaaccatt tttcagcgcc gtgggtaatg cggtgtcaac
    14821 ttgtgcagcg ggcgtttgct ggcttaacgc ctggcgggtt tcatgcatag gcgcacaccc
    14881 ggccagcatc agtaacagcg gaaaacaggc gatggctgga taaaaggaat cacgattcat
    14941 gggggaataa tcaggtaaga aacggtgcgc ggagattacc gtgtgttgcg atatattttt
    15001 tagtttcgcg tggcaataca tcagtagcaa taaaacgaca tacccagaaa aatatagact
    15061 aagtaaatga tatcttccga tttatcttaa tcgtttatgg ataacggcaa agggcttcgt
    15121 tttttcctat acttattcag cactcacaaa taaaggaacg ccaatgaaaa ttatactctg
    15181 ggctgtattg attattttcc tgattgggct actggtggtg actggcgtat ttaagatgat
    15241 attttaaaat taattaatgt catcaggtcc gaaaataacg agaatatttc agtctctcat
    15301 cctgttgcgc tcctgtcatg tgcattgctt catataatca ctggcgcaag gagcgcgcag
    15361 ggggcggcca atcgccgccg ccccctgcac ccccgggctc tggcgaacaa aatcgccgct
    15421 gcgcggtgcc ctcggcttat cccttacggc taccgggtcg ggcgcgaggt aacatccctg
    15481 taaaacgcgc cctcagccca cctccatgtg ggctgccccg gccttcaggg aacgcctcgg
    15541 caattttgac gccaccaaac aaccgtgcgg cctattgata aagagctaac acattgtcaa
    15601 aaaacatcac tatggttttt tagagtttct cgatatcaat tgcctgaata gcccttgcaa
    15661 tatcagggga attattcaac acccgaacat gctgaaataa ttccgttgct tcatcgtatt
    15721 ctttacgcaa ataactcaac cactgtttaa tccgcgcaac gtgatataac ccggtatcgc
    15781 cctgcttttc cagacgggta tatttttgca gcaaagcaac cacctccggc cacggcattc
    15841 gcggttcgtt atattttact acccggctca ggttgggaat attgagcgcc ccgcgaccaa
    15901 tcatcactgc gtcgcagccg ctgatcgcaa tgcattgttg cgcgctctgc cagtcccaga
    15961 tttcgccgtt ggcaatcacc ggaatattca gccgctggcg aatctcgcca atcgcctgcc
    16021 agtcaatatg ctccgcgcgg taaccctgct cttttgtccg tccatgcacc accagctccg
    16081 tagcaccagc ctgttgaacc gcatcggcga tttcaaattt cttctcaccg ctgtcccagc
    16141 ccagacgcac tttcacgctg acgggcaaat gcgccggtac agcttcacgc atcgcttttg
    16201 caccctggta gatgagttca ggatctttga gtaacgtcgc cccgccgccg ctgccgttaa
    16261 ccgttttcga cgggcagccg caattgagat ccacgcccca ggaacctaat tccaccgcac
    16321 gggcggcgtt ctctgccagc cattgtggga actgacctaa caactgaacg cgcaccagcg
    16381 taccggatgg tgtccggctg gcgttttgta gctcagggca aatgcgatga aagactttta
    16441 ccggcagcag ttgatccacc acgcggacaa actcggtgat gcacagatcg tagtcgttaa
    16501 cttcggtcag caattctcgc accagagagt caagcacacc ctccatcgga gccagtaaca
    16561 cacgcatatc ttcacccgga aaaaaatgag gcgctatgtt agcgcctatg gtcaacggat
    16621 taaaggtctg caaagaggga ttgctgcaaa ctgtaatccc tctctatgct tgatgtatgc
    16681 ctgagttcgt tgcgcattgc gtaatgccgg ggagcgaaaa aataagcgct gatttcttaa
    16741 tgtgatcggt agcacgtttt acgaattaat tgtatgatga atccatctca tctggggtgt
    16801 tgattatgag taagacactg aacattatct ggcaatattt acgcgctttc gtcctgattt
    16861 atgcctgcct gtatgcaggc attttcattg cttccctgct accggtaacc attccgggca
    16921 gcatcatcgg gatgctgatc ctgtttgtcc tgctggcctt gcaaattctt ccggcaaaat
    16981 gggtcaatcc ggggtgctac gtactgattc gctatatggc gctattgttt gtgccgattg
    17041 gcgtaggcgt catgcaatat tttgatttgc tccgcgcaca gtttggcccg gtagtggttt
    17101 cctgtgcagt cagtacgctg gtggtttttc tggtggtgag ctggagttca caactggtac
    17161 acggtgaacg taaagtcgta ggtcagaaag gatcagaaga atgatggcga atatctggtg
    17221 gtcattaccg ctgacgttga ttgtcttttt tgccgcccgc aaactggcgg cacggtataa
    17281 gtttcccctg cttaacccgt tgctggtagc aatggtggtg atcattcctt ttttaatgct
    17341 gaccggcatc tcttacgaca gctactttaa aggtagcgaa gtgctaaacg atctgctgca
    17401 accggcggtc gtcgcgctgg cctatccttt atatgagcag ctacaccaga tccgcgcgcg
    17461 ctggaaatcg atcatcacca tctgttttat cggcagcgtg gttgcgatgg tgacggggac
    17521 ttccgtggca ttgttgatgg gcgcttcacc ggaaatcgcc gcgtcaatcc tgccgaaatc
    17581 agtcaccacg cctattgcaa tggcggttgg cggcagtatt ggcggtattc cggcaatcag
    17641 cgcagtttgc gtgattttcg tcggcatcct cggcgcggta tttggccata cattgcttaa
    17701 tgcgatgcgt attcgtacca aagctgcgcg cggtctggca atggggacag cctcgcacgc
    17761 cctcggtacg gcgcgctgcg ccgagctgga ttatcaggaa ggtgcattta gttcgctggc
    17821 gctggtgtta tgcgggataa ttacttcgct gatcgcaccg ttccttttcc cgattattct
    17881 ggcagtaatg ggctaaaatt tgcgatgcgt cgcgcatttt tgatgtaagt ttcacgcgtt
    17941 gcataattaa tgagattcag atcacatata aagccataac gggttcgtaa actgttatcc
    18001 cattacatga ttatgaggca acgccatgca tccacgtttt caaaccgctt ttgcccaact
    18061 tgcggataac ttgcaatctg cactggaacc tattctggca gacaagtact tccccgcttt
    18121 gttgaccggg gagcaagtct catcgctgaa gagcgcaacg gggctggacg aagacgcgct
    18181 ggcattcgca ctacttccac tggcggcggc ctgtgcgcgt acgccattgt cgaattttaa
    18241 tgttggcgca attgcgcgcg gtgtgagcgg aacctggtat ttcggtgcca atatggaatt
    18301 tattggtgca acaatgcagc aaaccgttca tgccgaacaa agcgcgatca gccacgcctg
    18361 gttgagtggt gaaaaagcgc ttgcagccat caccgttaac tatacgcctt gtggtcactg
    18421 ccgtcagttt atgaatgaac tgaacagcgg tctggatctg cgtattcatc tgccgggccg
    18481 cgaggcacac gcgctgcgtg actatctgcc ggatgccttt gggccgaaag atctggagat
    18541 taaaacgctg ctgatggacg aacaggatca cggctatgcg ctgacgggtg atgcgctttc
    18601 tcaggcagcg attgcggcgg caaaccgttc gcacatgcct tacagtaagt cgccaagcgg
    18661 tgtcgcgctg gaatgtaaag acggtcgtat tttcagcggc agctacgctg aaaacgccgc
    18721 attcaacccg actctgccac cgttgcaggg ggcgttaatt ctgttgaatc tcaagggtta
    18781 tgattacccg gatatccagc gcgcggttct ggcagaaaaa gccgatgcgc cgttgattca
    18841 gtgggatgcc acctccgcaa cgctgaaagc tctcggctgt cacagtatcg accgcgtgct
    18901 tctcgcttaa gcctggtgcc ggatgcggcg tgaacgcctt gtccggcttg ccagccctct
    18961 cttggtgtcg aaattcccgg caaacagttt gccgtttctt gcgcaaaacc agcgggtaaa
    19021 gtagcctgat ggaaattttc cttagatcga gtctcctgca tgttaaagcg cgtgttcctc
    19081 agcctgttag tcctgatcgg cttgctgctg ttgactgtgc tcggcctcga tcgctggatg
    19141 agctggaaaa ccgcgcctta tatctacgac gaattgcagg atctccccta ccgccaggtc
    19201 ggcgtggtgc tcggcacagc aaaatattat cgtactggcg taattaatca gtattatcgc
    19261 taccgcattc aaggagcgat taatgcctat aacagcggta aggtaaatta tctattactg
    19321 agcggcgata acgcattgca aagttataat gagccgatga ccatgcgcaa agatttaatc
    19381 gctgctggtg tcgacccatc agatattgtt ctcgattacg caggctttcg tacgctggat
    19441 tccatcgtgc gtacacgcaa agtgttcgat actaatgatt tcattattat cacccaacgt
    19501 ttccactgtg agcgggcatt atttattgcg ctgcatatgg ggattcaggc tcagtgttat
    19561 gccgtaccgt caccgaaaga tatgctgtca gtacgtattc gcgaatttgc cgcccgtttc
    19621 ggtgcgctgg ctgaccttta tatttttaga cgtgaaccgc gttttttagg gccgctggtc
    19681 cctattccgg ctatgcacca ggtgccggaa gatgcgcagg ggtatcccgc cgtcacaccc
    19741 gaacagttac ttgaattaca aaagaaacaa ggaaagtagt tatggatgta cagcagtttt
    19801 ttgtcgttgc cgtttttttc cttatcccga tattttgttt ccgcgaagca tggaaaggct
    19861 ggcgcgcagg cgcgattgat aaacgggtta aaaatgcacc ggaaccggtg tatgtctggc
    19921 gagcaaaaaa tcccggactc tttttcgctt atatggtggc atatatcggc ttcggaattt
    19981 tatctatcgg catgattgtt tatcttattt tctatcgtta atattttctc ttttctgtta
    20041 attattgaga ataattatta cttcactcaa taagctgcgg atataacatt ctgaaccgca
    20101 gctatttgtg aatcctttca cagtttaaat ttccccacac gcttagcctt aatatcagca
    20161 cgcaatattt atttagcgtt cgccttaatt actcatagca ttaaggaaga tcacatgccg
    20221 caacaaaatt atctggatga actcactccg gcttttacgc ctttactggc gattaaagaa
    20281 gcctctcgct gtttattatg tcatgacgct ccctgtagtc aggcttgccc ggcacagacc
    20341 gatccgggga aatttattcg ctcaatctac tttcgtaatt ttaaaggcgc tgccgagaca
    20401 attcgcgaaa ataatgccct cggtgccgtt tgtgccagag tgtgcccgac ggagaaatta
    20461 tgccaaagcg gttgtacccg tgccggtgtt gatgctccca ttgatatcgg ccgcttacag
    20521 cgttttgtta ctgattttga acaacaaacc gggatggaaa tttatcagcc cggtactaaa
    20581 acgctcggca aagtcgcgat tattggcgca ggtcctgccg gattgcaggc cagtgtgaca
    20641 ctgacaaacc agggttatga cgtcacgatc tatgagaaag aagcacaacc cggtggctga
    20701 ttgcgtaacg gcattccgca attccggtta ccgcaatcag tactggatgc agagatcgcc
    20761 cgtattgaaa aaatgggcgt gaccattaag tgcaacaacg aagtcggtaa cacactcacc
    20821 cttgagcagc tgaaagcaga aaaccgcgcg gtactggtca ccgtggggtt atcaagcggt
    20881 tccgggctac cgctgtttga gcatagtgac gttgagattg ccgtcgactt cttgcaacgt
    20941 gcacgacagg cgcaaggcga tatcagcatt ccacaaagcg cattaattat cggcggcggt
    21001 gatgtcgcga tggacgtagc cagcacgctg aaagttctcg gctgtcaggc ggtaacttgc
    21061 gtagcgcgtg aagagttaga tgagtttccg gcaagcgaaa aagagtttac cagcgcccgg
    21121 gagctgggcg tttcgatcat cgatggattc acgccagtag ccgtggaagg caataaagtc
    21181 acgtttaagc atgtacggtt atcgggcgaa ctgacgatgg cggcagataa aattattctc
    21241 gccgtcggtc agcatgccag actagatgcc tttgcggagt tagagccgca gcgtaacacc
    21301 atcaaaacac aaaattacca gacccgcgac ccgcaagtct ttgctgctgg cgatattgtt
    21361 gagggagaca aaaccgtggt ctatgccgtg aaaaccggga aagaagccgc cgaggcgatt
    21421 catcactatt tagagggagc ttgctcatgt taacgaaaga tctttcgatt actttttgcg
    21481 gcgtgaagtt tcccaacccg ttctgcctct cttcttcgcc ggtaggcaac tgctatgaga
    21541 tgtgtgccaa agcctacgac acaggttggg gcggtgtggt gtttaaaacg atcggctttt
    21601 ttatcgccaa cgaagtctcg ccgcgttttg atcatctggt gaaagaagat accggtttta
    21661 tcggcttcaa aaatatggag cagattgccg agcatccgct ggaggagaat ctggccgccc
    21721 tgcgtcggct gaaagaagat tacccggaca aagtattgat cgcttcgatc atgggggaaa
    21781 atgagcagca atgggaggag ctggcgcgcc tggtgcaaga agctggcgcg gatatgatcg
    21841 agtgtaactt ctcctgtccg caaatgactt ctcatgcgat gggtagcgat gtcgggcaaa
    21901 gcccggagct ggtagaaaaa tattgtcggg cagtgaaacg gggttccacg ctgccaatgc
    21961 tggcgaagat gacgccgaat atcggtgata tgtgcgaagt ggcgctggcg gcgaagcgcg
    22021 gcggcgcaga tggcattgcg gcgattaaca ccgttaaatc catcaccaat atcgatctta
    22081 atcagaaaat cggtatgccg atcgttaacg gaaaatcaag tatttccgga tattccggta
    22141 aagcggtaaa accgatcgcc ctgcgcttca ttcagcaaat gcgcacccat ccagaactgc
    22201 gcgatttccc aatcagcggt atcggcggca ttgaaacctg ggaggatgcg gcggagtttt
    22261 tattgctcgg cgcagcaacg ttacaggtca ccaccggcat tatgcagtac ggctatcgca
    22321 tcgtagaaga tatggcgagc gggttatcgc attatctcgc cgatcaggga tttgattcgt
    22381 tgcaggagat ggtaggtctg gcgaataaca atattgtccc ggcggaagat ttagaccgca
    22441 gttatattgt ctatccccgt atcaatcttg acaaatgtgt tggctgtgga cgctgttata
    22501 tttcctgtta cgacggcggt caccaggcga tggaatggag cgagaaaacc cgcacaccgc
    22561 attgtaatac cgagaaatgt gtgggttgtc tgctttgtgg tcacgtctgc ccggtgggtt
    22621 gtattgagct cggggaagtg aagtttaaga aaggcgagaa agaacacccg gtaacgttgt
    22681 agaacccgcg tcacatccgg catttggttc ctgttgccgg atgcggcgtg aacgccttat
    22741 ccggcctaca aaaccacaca gatcatgatg ttatgtgcgt ttatgaccga atgcggacca
    22801 cattcacatc atttcttacg cgcgtatttc agtgaatcca gcgctacggc gaagataata
    22861 atcgcccctt tgatgatgta ctgccagtat gggtttacgc cgatatacgt caggccatag
    22921 ttgatgacgg taaaaataat caccccggtc accacgccaa tcaccgtccc cacaccgccg
    22981 ctgaacgata caccgcctac cacgcacgcc gcgatagcat ccagctcata cataaagccg
    23041 aggttgttgg tggcagagcc gatacgtccg gcttctaaca tcccgccaaa ggcatagaac
    23101 acgccagaca acgcgtagat catcagcagg ttcaggccga cgttgacacc agatactttt
    23161 gccgcttccg ggttaccgcc aatggcaaaa atgttcttac cgaagcgggt tttgttccac
    23221 aacacccaga cgaacgccac cgcaatcaac gcgtagaagg tgatgtaaga gagacggaaa
    23281 ctccccagcg cgacaaagcc ctgagcaaag gtagagaagc cactgtcaaa accagaaatt
    23341 ggcgacgccc cgacaaagtc gtaatagagc gagttgatgc catagacgat gatcatcgtg
    23401 ccgagcgtgg taatgaacgg cgtcacgttg agataagcga taatcagacc gttgatcaaa
    23461 ccgatcaccg caccaatggc acagacaatc agaataacca gcgcaatcgg catcgtcgcc
    23521 atttccggga acactttgtt ggcgttatcc atggactgca ataatgtcgc agccaccact
    23581 gccgccagcc ctacctgacg accagcagaa agatcggtcc cctgggtgac aattaaccct
    23641 gccacaccga gcgcgataat aatacgcacc gatgactggg tgagaatatt acttaagttc
    23701 aacagactta aaaatgttgg gtcctggaaa ataataatcg ccagcaaaac taaaagaacg
    23761 acgtaaatac cgccctcttt caggtaagta agaaaacttt tcttatttaa cgcactcatg
    23821 ggaagcccct aatcttaaag gtgcaaagac gcaagacgca gaatttcgtt ttgcgttgtt
    23881 gtttttgtat cgacaattcc ggaaacgaga ccattgctca tgaccagaat acggtctgtt
    23941 atccctaaca actcaggcat ttcagaggag ataataataa tccccttgcc tttcttcgcc
    24001 agttcggcaa ttaactgata aatttcaaac ttcgccccga catcaatacc gcgcgtcggt
    24061 tcatcgagca ttaatatttc tggttgcgtt agtagccagc gaccaataat caccttttgc
    24121 tgattaccac cggagagcga accaatttgc gtccgatgac ccggcgtttt tacccgcatc
    24181 gaatcaatca cccactgggt atcgcttttc atccgcgagt tatccagtaa accaacttta
    24241 tttttgtagt tgcgaatatt ggaaattaac gagttaaaac caatatccag ataggcataa
    24301 attcccgttg agcggcgctc ctcagttacc agtgcaaatc cgtggtttat ggcttcgttg
    24361 gcattatggt tattgatctg tttgccgtgc aacgtaatgg tgccagccga tttctcgcga
    24421 ataccaaata acgtctcaac aatatcggta cgtttcgccc ccaccagccc ggcaataccg
    24481 aggatctccc ctttatgcag atcaaacgag acatcgcgaa tcgacggctg gcgcagtgac
    24541 gttaggttac gcacctcgag gatgacttcg cccggcttgt tttctttgtc aggaaaacgc
    24601 tgattaagag aacgcccaac catcatggcg atgatcttgt ccatcgtcag tcctgccagc
    24661 ggttcggtgg cgatccactg accgtcgcgc aatacggtaa cttcatcaca taactggaag
    24721 atttcttcca ttttatgcga gatataaaca ataccgcagc cgcgctcttt taatttacga
    24781 ataatagtga acagatgatt gacctctttt tcggttaacg aagaagtcgg ttcatccata
    24841 atcacaattt tcgcgttata ggaaaacgct ttggcgattt cgatcatctg catttgcgaa
    24901 acggataatg tgccgacgcg cgcacgcgga tcgatatcaa tatccagttc atcaaaaatt
    24961 gctttggttt cgcggtacat tttgtcctga tcgacaaaca tgcctttggt gggatatcgc
    25021 cccagccaca tgttatccat caccgaacgt tgtaatacca ggtttaactc ctggtgtacc
    25081 atcgaaatac cattttccag tgcctctttg gcggaatgga aatcgatctc tttaccctgg
    25141 aataaaatgg tgccggagtc tttttgataa ataccaaaca ggcattttaa taatgtcgat
    25201 tttcctgcac cgttttcccc cattaatgca tggatagaat gtggccggac ttttaaatta
    25261 acgttatcaa gtgccttaac accaggaaag gacttgttga taccgctcat ttccaacaag
    25321 tattccccgg aagacggagt cgttgagctg accatataat tttaccttgt tggccataca
    25381 ataagggcgc agtaaacgac tgcgcccaat cagtcttatt tcttgctgaa ttcagccagg
    25441 ttgtctttat ctacgccaac ataaggtacg cggaccactt tgttgtcgat tttccagttg
    25501 gtgccatcag ccgcaccttt gccatcggcc aggtttttcg ccagatcaaa ggtcgctttc
    25561 gcctggttgt tagcatcgtt cagtactgtg cccgccagtg caccggattt caccagcgcc
    25621 agcgcttctg gcagcgcatc gacgccaaac accggaatgc tggacttgtt gtgtgctttc
    25681 aatgcttcta ccgcgcccat tgccatcgca tcgttgttgg cgataaccac ttcgattttg
    25741 ttggcgttcg ggccagacag ccaggcgtcc atcttatctt tcgcctgagc ggtgtcccac
    25801 attgcggtat ctaactgtaa ctgttcagtt ttgatgcctt tatcgttcaa ttctttaatc
    25861 acgtaagtgg tacgtgcttc tgcatccgga tggcccggtt cacctttcag cagtacgaac
    25921 tgaatctgac cgtctttgtt cagatcccaa ccctgattcg ccgcccagtg tttagcaatc
    25981 aaatcgccct gaataatgcc ggactctttg gagtcagtgc caacgtagta ggctttgtcg
    26041 tagctatcca gcgccttacg agacggttct ttgttgaaga aaaccaccgg cacgttttgc
    26101 ccacgcgctt tctcaatcac cgtacccgca gctgccgggt caaccaggtt gattgccagt
    26161 gccttcaccc ctttcgccag caatacgtcg atctgatcgt tctgcttgga ctggtcattc
    26221 tgagaatcat tcatcagcag ctgaacatct ggcgcggctt tcgcatcttg ctcaatagcc
    26281 ttgcgcacta cagacataaa gttatcgtcg tacttataga ttgttacacc aatgcgagta
    26341 tcagcagcgt gtgcagcggc accgaataac atgctggcca tcacagcaga cagggttaac
    26401 accttcttat tcatggtatc tccggttttt cttatgcagg gtagtgcttg agataaatgc
    26461 tcggcggggc agtagagtta atgaagtgtt actgaacgcc gaagctcact tttttaaatt
    26521 cgttcttcca tgcccggtaa cgctccagaa aacggcttta attgttgtta tgacgctatt
    26581 acctcggtga aagtgattat tcactgttac atacgggtta caacgttaaa acggtgcaat
    26641 catagctatc acattgttaa gatactgtga aatcactcac agattgaaag cggttacatc
    26701 gcctgatttg ttgagttagt gatcgccgcc gcattctggc gcgttgcgac agaatggcga
    26761 cgcactaacg tcggcataaa acagtggctg gcacgaggat caatattgcc tgctgccccc
    26821 tgcaaggcca gttcggtggc taatttagcc attgaagcaa tgggataacg cacggtcgtt
    26881 aattgcgggt cggtgtaacg ggcgatggga atatcatcga aaccgatgat tgagagatgt
    26941 aacggaatcg caatgccatt atcttttaat gctgtcagtg cgccagcggc catattgtcg
    27001 ttataagcaa atatagcggt aagttgtaga ttgcgcccca gcagttcaac catcgccgcc
    27061 tcaccgcccg gcatgtccgg cgtaccagtg ccaatccagc tttccggcgg aataatatcc
    27121 tgctctttca acgcactcat ccagcctgct ttacgcatgg cgtcatcttc aatgccgtga
    27181 ctggaagaaa gataaccaat acgttgatga ccgttattca gcaacatgcg cgtcgccatt
    27241 cgggcaccgc tgagattatc caggcaaacg caacgatggg cgtaccccgg cacaacgcgg
    27301 ttgattaaca ccataccggg aatgttatcc ataaattgcg ccagttcatc gtcactcaat
    27361 gcttttgagt gaacaatcaa cgcattacaa cgctggcgaa ttaacacctc aatggcgtga
    27421 cgctcttttt ccgcttcatg atagctattg ccgattagca cgtatttctg atgctgctga
    27481 gcgactagat ccaccgcttt taccagcgcg ccgaaaaacg catcagaaac gtccatcacc
    27541 accacgccaa tggtgtcgct aacctgagtt gccagcgcct gggcattggc gtttggccga
    27601 taatccagct cactcacggc tttcattact gcttcacgcg tgtcggcact gaccagcgtg
    27661 ctgttattga gcacccggga aaccgttgcc acagagacgc cagcctaacg cgctacatca
    27721 cgaatggtga tcatattcac tacctgttat gagattgcag taaatgactg cttgctggcg
    27781 gctattttgt cagcactgaa tacaggactt cgtgaatcga gtcacagcaa tggaaacggt
    27841 tacagccgtt ttgttaacga atgtgatcca gatcgttatc ttgatgtttt agatattgcc
    27901 gttccggcag cacgcaaagt taactgacgc cagagccatt ccaccggccc ctggcggaag
    27961 taacgcagcc agataacaga gaagaggata ttcgccagcc ataccggaat aacaaacgcc
    28021 agcagctcca ggcggtcaaa atgcataaac aaaccgaggt ggtaaaaaag catggtacaa
    28081 atcagcgttt gcaatagata gttggtcagc gccatccgtc cgacgcaggc gatcgcaagc
    28141 accagcttaa agcggctcaa ttgcggccag aagccataaa acagcgacgc atagccaatc
    28201 gcctgaaacg gcgcactcag ttcccgcggc atttgaagta agaaggcaca ccagcgatac
    28261 gcccagtcca gctgccattg cagggcgatg gcaggaaggt taatggtcac cccaatcgcc
    28321 accagcacaa aaccagtacg acgatagtga cgtaagctga actgcccttt cagccagccg
    28381 ctgcgcatca gcgccgcacc aatgagcatc atccctgcca gttgccagcc atactgtgcg
    28441 cccagtgcca gtaaactgtt gccaacacca tcggcacgat tactgatcgc ttcaacgccg
    28501 ccgtgaagct tccagtattt ttcatacaaa atagccgatg catccggcgt ccaggtgcgg
    28561 ctggtctggc tgtcggaaat cagccccaat aacagcaaaa cgccaagccc caccagataa
    28621 agcatgacgc ctgtattaaa caggcttttt accgatggcg catcgcgcac cagccgccag
    28681 cagattaagc ccaccagccc gtaagccagc agaatatcgc cgtcccagaa caataaaccg
    28741 tgaataaaac ccagcaagac taacagcgtt aaccgcgact ggatccagcg tctgccacgg
    28801 ggcagcaaca tttgcaggcc cgcaccaaac agcagcgcaa aaagcgtgag gaatttcacc
    28861 tggccgatga gatcgagaaa tgcccaggtc catgcatcct gcggcgtaat agcgccgtac
    28921 caggcgggat tgagataagc cgcctttggt agcccaaagg cgctgatgtt taatagcagg
    28981 atccccagaa tggcgacgcc gcgaacaaaa tcgagcgtta cgttgcgctc catggttcct
    29041 gccttttaat cagttgtgat gacgcacagc gcgcagaaac tcgtggcgcg tattctgact
    29101 ggatttgaac aatccaccaa gagaggtcgt tgttgtggca ctggttgcat cgcggatgcc
    29161 acgcgctttc acgcagtaat gcaccgcatc aatggagaca gccacgttat tggtgcccag
    29221 cagcgtttgt agcgcaataa gaatttgctg cgtcagacgt tcctgcacct gcggacgctg
    29281 ggcaaagaac tgcacgatac ggttaatttt tgacagaccg atcaccgaat ctttcgggat
    29341 ataagccacc gtcgctttgc catcgatggt aacaaaatgg tgttcacagg tactggtcag
    29401 agtgatatcg cgcacggtga ccatttcatc gaccttcatt ttgttttcaa tgagggtgat
    29461 tttcgggaag ttggcgtaat ccagaccgga gaaaatttca tcgacataca ttttagcgat
    29521 gcgatgcggc gtttccatca aactgtcatc agccaggtcg agattcagca gctgcatgat
    29581 ttcggtcata tgaccagcaa taaggctttt gcgcgtttcg ttatccattt catgcacggg
    29641 cgggcgcagc ggtgtttcca gtcctcgcgc aactaacgct tcatgaacca gggccgcttc
    29701 tttactgagt gatggcattt atgatttctc ctgcaggtgt gacgcctccg ccctgcgtgg
    29761 gggcaaagtt attaagctga tttacagcct gattattgtg cgtgaggcgg cgcacataat
    29821 ccagtattca cagcgataat tattgtaatt gccgctgact ttcatcagca gatgttaaaa
    29881 catcgttatg caaatacgga agtgaaagtt actcacagca cattgaataa acggtatgat
    29941 gaagaaattg caaacaacac aacaaggagc cacgcatgga aatgctcgaa gagcaccgct
    30001 gttttgaagg ctggcagcaa cgctggcgac acgactccag taccttaaac tgcccgatga
    30061 cgttcagtat ctttctccca ccccctcgtg atcacacccc gccaccagtg ctgtactggc
    30121 tttccggatt aacctgcaat gacgagaact tcaccaccaa ggcgggtgcc cagcgcgtgg
    30181 cggcagaact ggggattgta ctggtgatgc cagacaccag cccgcgcggc gaaaaggttg
    30241 ccaacgacga tggctacgat ttaggccagg gcgcaggctt ttatcttaat gccacgcaac
    30301 cgccgtgggc gacgcattac cggatgtatg attatctgcg cgatgaatta ccggcgctgg
    30361 ttcagtcgca atttaatgtc agcgaccgct gcgccattag cggtcactca atgggtggtc
    30421 acggtgcgct gattatggcg ctgaaaaatc cgggtaaata caccagcgtt tcggcctttg
    30481 cgccaattgt gaatccgtgc agcgtcccgt ggggaatcaa agcgtttagc acctatttag
    30541 gtgaggacaa aaatgcatgg ctggaatggg atagttgcgc gctgatgtat gccagtaacg
    30601 cgcaggatgc gatcccgacg cttatcgatc agggcgataa tgatcagttt cttgccgacc
    30661 agttgcaacc tgcggtattg gcagaagccg cgcgccagaa agcgtggccg atgacgctgc
    30721 gtattcagcc gggatatgat cacagttact acttcatcgc ctcttttata gaggatcacc
    30781 tgcgcttcca tgcgcagtat ttactgaagt gaaagtccgc ccggttcgcc gggcatcttc
    30841 tcatcagaag cgataatcca ctgccataaa gtaacgacgt ccgtcttcgt tatagctgta
    30901 gtcgtcacga ctgagatcct tgtcgccaag gttcagcacg cctgcgcgca gtttgacgtc
    30961 tttagtcacc tgccaggccg cgccggtatt ccagatggta taaccgcccg gtgttttagc
    31021 cgtcgcgctg tcggcgcgtt tctgcccggt atagtgacca gaaacataga atgaccagtc
    31081 ttccagcgcc agcggtttcc agtccagcgt accgttagca gtatggaacg gcagatcgga
    31141 tagcggtttg ttttcgccgt tgctgacatc acgaccatcg ttgtaggtgt agttgatcga
    31201 cagtttccat tcatcgttga acggaatttt cagttcggtt tccacgccct gaatacgagc
    31261 tttgttaacg ttgtagtagc taaataccgg tatgcgccgt ccgttagcgc ccgtctcaaa
    31321 accaacaaag ttttggtagc ccggtgcagc gttgacgtca gacgtacggc tgatgctgat
    31381 acgatctttc acatcgttac ggaaaacggt aacgctggat tcaacccctt ccagccagcc
    31441 ttcttcaccc atgtagtaaa gccccagctc ccaactttcg ctggtttctg gtttcagatc
    31501 cgggctaccc acaatcttac atgcgccacg gcaggaattg ctcgtccagt cagggctaag
    31561 ttgcaacaga gaaggtgctt taaatgccgt cgcccagccc cctttcaccg ttacggtgtc
    31621 ggtggcgtta taaaccaggt aggcacgcgg actccagtgt tcaccgtagg tttcgtgatc
    31681 gtccatacgc acgccggtcg tcagcgccag cggctcgaag atccgccatt catcttccac
    31741 aaacagcgcg tactggctgg cagacgtttt ggagctggtt cccccggtca ggttcaccgc
    31801 atcgctaagt ttgtcgtgac gccattcacc gccaaccgtg agaaactgat taatcgccgt
    31861 cagcggcaac gtgtatttgc cgtcgaccgt attgctttcg gaagttatcg ggctgctgtt
    31921 gccagggttt ttgttctcga ctttctcacc gtagtatttc agttcgctgg tgccgtaatc
    31981 ccaacgccca ttatggctga cggagtagtt ctggcgttcc aggcggtttt tgtccagcga
    32041 gtcggaatca cgatcctgac ggtcgaaacc gtatccggca gtaaaatcgt gattttgatt
    32101 cggtgtccag gcaaattcga cattgccgtc gcggctggag aatccttcaa tacgcggcgt
    32161 ttctccggta tcggtggtcg ttgagttttg cgggtcatcc ttttcacgtt ttgccaggct
    32221 gccgtaagct ttcattccca gcacaccatc aattaatggt ccactggtaa agaactgacc
    32281 gttataggta tcaccgcgat cgcgatgttc ctgaatggtg gtatcgacgg taacggtacc
    32341 cgaccatttc tgaccgattt ttttggtgat gatattcact acaccgccga gcgcatcgga
    32401 accgtacagc gacgacatcg ggccacggac cacttcaata cgttcgatgg aatcgaccgg
    32461 gatccagttc agatcgaaat cattgtggcg gaagacggca ttgcgggagt tcacgcgttt
    32521 accgtcgacg agaatcaggg tatagctgct gtccagacca cgaatactaa cgcccttacg
    32581 gttatcccct tcgttcgtca gttgtacgcc aggcacttct ttgaggacat ccttcagatt
    32641 ctgtaccggt tttcgctgca gatcttcctg ggtaatgacg ctgatactgg cgggtgcatc
    32701 tttaaggttt tgttcaacgg aagatgcagt gacaaccatc gtttcgccat cgtcatcgac
    32761 cgctaacaca ggccatacac aagaaatagc ggacaaacac agcccgaccc gtacgaaagg
    32821 gttcaaccta aacattccat atctccatga ggtaactacg aaaataaaat gggtatcgct
    32881 cacatcttct ttacgtcccc ttgcgtacgg cagcatcgcg gtggacttat ttgtatgcag
    32941 gtgatcatcc cagaaagccg gaccagcctc tgatttgtca gccttttttg attgtagcgt
    33001 aacgataatg caaacgataa caattatcaa tccaaatgtt aaattttata tccgcatgat
    33061 ttgtcaggga aatggaattg aaaaagccct ctcggttgag agggcttagc aaggaaggga
    33121 ggaaacttat ttcttatcgt tctgcgggaa cttcatttcg ctgtagcgta cgaagtgagt
    33181 tcctttaatc agcttgtagc cgaaccaaat aatcaggaac agcgggatac caatatacgt
    33241 tgccgctacg ccgccccagt caatggtatc tttcaggaac gcttcgtagt tctggcccaa
    33301 agtgataatc agacacagaa tgaatgcgaa gatcggcccc agtgggaaga aacctgaacg
    33361 gtacggcaga tcgttaatgt cgtgtccctg caatacgtaa ccgcgacgga agcgatagtg
    33421 gctaatggca atccccagcc aggcgataaa acccgtcatc ccggaggtgt tcagcagcca
    33481 caggtatacc gtctggttgc caaacatgga ggtcaggaag cacaaaccgg caatcaccgt
    33541 cgtcgcatac agcgcattac gcggcacgcc accacgcgac aatttagcga aaatgcgcgg
    33601 cgctttaccg tcacacgcca gggtgtacag catacgagta gacgcataca taccggagtt
    33661 acctgctgac agcaccgccg tcagaataac tgcgttcatc accgccgccg cagagagcag
    33721 acccgcgtgc tggaacacca aggtgaacgg actgacgctg atgtctttaa catcgttacg
    33781 cagcaggctc ggatcggtat acggaataat caggctgata atcaggatcg cgaacacata
    33841 gaacaacagg attcgccaga atacctgacg taccgcgcgt ggaatgtttt tcgccggatc
    33901 ttcggactcg cctgcagcaa taccgatcag ctcggttccc tggaaagaga agccgacaat
    33961 catagctacg ccgatcatcg ccgcaaaacc accagcaaac ggcgcttcgc cgattgtcca
    34021 gttgctccag cccgcaggct gcgcgccttt gaagataccg ataatcatca gcacgccaac
    34081 gatgataaag acaataactg tcgtgacttt gatcagtgaa aaccagtatt ccgcttcacc
    34141 aaagccacga actgagatgt agttcagcag gaagataacg ccgaggaaca acgcactcca
    34201 gatccagccc ggtgtatccg ggaaccacca gctcatgacc agctgtgctg caaccaagtc
    34261 aacggcaata gtcaccgccc agttgtacca gtagttccag cccagcgcga agccaaagcc
    34321 ttcttcaaca tagttctgac cgtaagtggc aaacgaaccg gaaaccggca tatatgcagc
    34381 caattcaccg agactggtca tcaggaagta aaccatcagg ccaatcagca tatacgagag
    34441 caatgccccg cccggacctg cctgagaaat cgttgcgcca gaggcaacaa aaagacctgt
    34501 accgatggaa ccgccaatgg caatcatcgt caggtgacgc gcctttaatt cacggcgtaa
    34561 gcccggcgct tctgtagttt tagtttcgga acccatacaa aaatgctatc catcttaaaa
    34621 atgaggcgcg attgtagcag acgattggca atccttccgg cagaaataca cggttataag
    34681 acaccttcat gatcgcccag ggattataag taaagcagcc aatctctttt ctggcgaatg
    34741 catgctgagg gcgctgcgtc acgaaatcga cacagcacca gcatgttctt gtacagcacc
    34801 ttaacgcggt acattcgcgg gatcgcaata gtccagaaag cgccgtagcg cgttggaaag
    34861 gtgtttttgc cgatgatgta tacgccacaa cgtacgcatc aggcgcggga gagggaccgc
    34921 aacttcactt aatgtgcctg cctgcaattg atcttcaatc acacgtcgcg acaggcaact
    34981 aattcccaac ccatgacgca ccgcatgttt gattgcctcg gagttaccta attccatcgc
    35041 catctcaaac ttcggtaaat gtgacagcaa cagataatcg acaatctccc gcgtgccgga
    35101 accgcgttca cgcaggatcc acggcgcagc ggccagctgt tctaaggtga ccggaccacg
    35161 agccaacggc gaagtcggcg cggcgaaaac caccagctcg tcttccagcc acggttcaga
    35221 aatgatttca gtgctgtggc acggtccttc aataaagcca atatcaacgc ggaaatccag
    35281 caccgcttgc atcacgtcct ggctgttccc aacgctaagt tcaatcggca actgcggata
    35341 atcatggcga taacgggcga taactgcagg cagaatgtag ttaccgatgg tactactggc
    35401 atagatacga atcgcgccgt tgtcttcgcg aaacagttgt tcgatttcaa ccgcctgttc
    35461 aagcaatgcc aacgcacgcg gatagagcag ccgcccgtgt tcattaacaa ccagtctttt
    35521 ccccacgcga tcaaacagtt gcacgccaag ctgcccttcc aggtcggtca aggctgcgct
    35581 cactgctgat tgcgacaacg ccagcatcac cgacgcctgg gtggttgatc cacttttcaa
    35641 tacttctgca aaaacttcca actgccggag ggtgatgtgc atagtcgctt accacttata
    35701 aagattaatt ataaatatat aatcaatttt atttttaaac cagttagtcg taaccttatg
    35761 cccggttaaa ggagagggtt atgacgaata tcaccttaca gaaacaacat cgtacactgt
    35821 ggcattttat tccggggtta gccctgagtg cagttatcac cggggtcgcc ctgtggggtg
    35881 gttccattcc cgcggttgcg ggtgccgggt ttagtgccct caccctcgca atcttgttgg
    35941 ggatggtttt aggcaacacc atctatccgc acatctggaa aagctgtgac ggtggtgtgc
    36001 tgtttgccaa gcaatattta ttacgtctgg gtatcattct ttatggcttc cgtctgacgt
    36061 tctcgcaaat tgccgatgtc ggtatcagtg ggatcatcat tgacgtgttg acgctgtcca
    36121 gtaccttcct gctggcttgc ttcctggggc agaaagtgtt tggtctggat aagcacacca
    36181 gctggttgat cggtgccggt agcagtatct gtggtgctgc cgcggtactg gcgactgagt
    36241 cggtagtgaa agcggaagcc agtaaagtaa ccgtggctgt tgcaaccgtt gttatcttcg
    36301 ggaccgtcgc gattttcctc tacccggcga tatatccgct gatgtctcaa tggtttagtc
    36361 cggaaacttt cggtatctac attggttcta ctgtgcacga agtggcgcag gtggtggcgg
    36421 caggtcatgc catcagcccg gatgcggaaa acgcagcagt tatttccaaa atgctgcgcg
    36481 tgatgatgct ggctccgttc ctcatcctgc tggcggcgcg tgttaaacag ctgtctgggg
    36541 cgaacagcgg cgagaaaagc aaaatcacta ttccgtggtt tgccatcttg ttcatcgtcg
    36601 ttgccatctt taactcgttc cacctgttac cgcagagcgt ggtgaacatg ctggtaacgc
    36661 tggatacctt cctgctggca atggcgatgg cggcgctggg tctgaccact cacgtcagcg
    36721 cgctgaaaaa agctggggcg aaacctctgc tgatggcact ggtactgttt gcctggctga
    36781 ttgttggtgg tggtgcgatt aactatgtga ttcaaagcgt catcgcataa accactacat
    36841 cttgctcctg ttaacccgct atcattaccg ttttcctcca gcgggtttaa caggagtcct
    36901 cgcatgaaat acattggagc gcacgttagt gctgctggcg gtctggcaaa tgccgcaatt
    36961 cgcgccgtcg aaatcgacgc aaccgcgttt gccttgttca ccaaaaacca acgtcagtgg
    37021 cgtgccgcac cgctcacgac gcaaaccatc gatgaattca aagccgcctg tgaaaaatat
    37081 cactacacat cggcgcaaat tcttccccac gacagttatc tgattaacct cggacatccg
    37141 gtcactgaag ctctggaaaa atcgcgcgat gcctttatag atgaaatgca gcgttgcgaa
    37201 caactggggc tttctttgct caacttccac cctggcagcc atctgatgca gatttcagaa
    37261 gaggattgcc ttgcgcgtat tgccgaatcc atcaacattg cgctggataa aactcaaggt
    37321 gtaacagcgg tgattgaaaa caccgccggt cagggcagta acttagggtt taaattcgaa
    37381 catctcgcgg cgattatcga cggcgtggaa gataaattcc gcgtcggcgt ctgcattgat
    37441 acctgccatg ctttcgctgc cgggtatgat ttgcgtactc cagccgaatg cgaaaaaacg
    37501 ttcgctgatt ttgcccgtat tgtcggcttt aagtatctgc gcgggatgca ccttaacgat
    37561 gcgaaaagta cctttggcag ccgcgttgac cgccatcata gcctcggtga aggcaatatc
    37621 ggtcatgatg cgttccgctg gatcatgcaa gacgaccgtt tcgacggcat tccgctgatc
    37681 cttgaaacca tcaacccgga tatctgggca gaagagatcg cctggctgaa agcgcaacaa
    37741 actgaaaaag cggtggcctg atcgatgaac gacagggaaa agcagattct taaaatttta
    37801 cggcgtaacc cgctgattca acagaatgaa atcgccgata ttctgcaaat cagccgctca
    37861 cgtgttgccg ctcatattat ggatttgatg cgcaagggtt tgattaaagg caaaggatat
    37921 atccttaccg agcaggatta ctgtgtggtt gtgggtgcaa tcaatatgga tattcgcgga
    37981 atggcggata tccgttaccc gcaagcagcc tctcatccag gttctgttca ctgttccgca
    38041 ggcggcgtcg gacgtaatat cgcgcataac ctggctctgc ttggtcggga tgtgcattta
    38101 atatcagcta ttggcaacga tttttacggc gagacattac tggaagaaac gcgccgcgcg
    38161 ggcgtgaacg tttcaaactg cattcgtctg catggacaca gcactgcaac ttatctcgct
    38221 atcgctaata agcaggaaga aactatcctg gcaatcaatg acacacatat tctgcaacag
    38281 ctaacgcctc aactgctcaa tacttcacgg gacttgattc gccatgctgg tgtggtgctg
    38341 gctgattgta atctcacacc tgaagccctg gagtgggtct ttaccattgc tgatgaaatt
    38401 ccgatgtttg tcgataccgt ttccgaattt aaagcgaaca aagtaaaaaa ctggtacagc
    38461 cggatccaca ctctgaaacc aacacaaaat gaactggaaa ttttgtgggg gcaaccgatt
    38521 aaagacgaca acgatcgcat cagagctgta aattcgctgc atcaacaagg tgttaaacgc
    38581 atttttgttt atctgaaaga tgaatctgta ttttgcagcg ataaggatgg agaacaattt
    38641 ttgctgaccg cgccagcgca tacgacagta gacagttttg gtgctgacga tggttttatg
    38701 gcgggcctgg tatatagctt tcttgaagga tacagtttcc gcgacagcgc ccgttttgcg
    38761 gtagcctgcg cggcaatttc gcgcgccagc ggcagcttaa acaaccctac cctgtctgcc
    38821 gataacgcgc tttcattagt gccaatggtg taacaatgtt gccggattcg acgcttgacc
    38881 gcatcttatc cggctcataa attgcgcact acgctaaacc aataaagaat cctgcaatag
    38941 tagcactcat caggttagaa agtgtcgccg ccgccagcgc gcgtaaacca agttgggcga
    39001 tttccggcgc acggtgtggc gcaaccgcag aaaacgcccc caccaccacc ccgatagaac
    39061 caaagttagc gaaaccgcac aacgcgaaag aaataatcgc cacggtttta gcatccagag
    39121 tgccacccgt ttgcagatat ggcgagaaat tgagataagc gacaaattca ttgatcgcca
    39181 gcttctgccc aatcaaactc ccggcaagat ttgcatcact ccagtcaacc cccatcaccc
    39241 acgccaatgg ggccaacagg taacctaaaa tcgactccag cgaagcatgt tcaaaaccaa
    39301 accagccgcc aacaccgccg ataataccgt taatcaacgc aattattgca acaaatgcca
    39361 taacaactgt cgctacaccg gcggcgattt tcagcccggt cattgcccct gtcgcagcgg
    39421 cttcaataat gctttttggc ggtgtttcgg tgaaagagag gttattaaaa gaaacctgcg
    39481 aagattccgt agccgggctt aacaggcggg caaacaagat cccgccaggg atcgccatta
    39541 acgatgccgc cagcaaatat tccacaggta cgcccagtgc ggcgtaacca atcattgtcg
    39601 aaccagcaat cgaggccatg ccactacaaa tcgctgtaaa taattcattg cgattcagac
    39661 gatcgataaa gggtttgacg atcgccggaa tttcgttttg cccgaggaaa atggtggtga
    39721 ccgcgacgaa tgactcgatc ttgctgatat ttaaagcttt ctggaagata ccgccgagaa
    39781 tgcgaattaa gatccccatc acaccgatgt agtagagaat actcaccagt gcagtgacga
    39841 agataattgc cggtaatacc ctgaaaccaa agataaatcc tgcgccatca aataaggtat
    39901 ccattttcgg tccgaccaga gaaccgaaga taaatgcgct acctgcgtcg ctgtacgcca
    39961 tcactttgtg gacgccaaaa gcgacttttt ctgcgaccca acgccctggc ggtaaccaaa
    40021 gcataatccc gccaatcacg acctgtaaca ctaacgccgc gccaacggta cgcaggctga
    40081 tcttcttctt gtttactgac agtaaaaacg caattgccag cagtaccacc attcccagaa
    40141 cacttctcat gacatccata gtgattatct cttcatgtca ttttgggcga cggctttctg
    40201 cccatcagaa atagccgggc ctgcatcttg cgatactgtc ccggcttgtt atcaacactt
    40261 aatgggtttt tatgtagccg cgtacgcact cttcgactaa tccccagaac cagtcggtat
    40321 caatagtgat gccgacttta gtattggcgg gcttgccaag aacgcccagc tcgtcgcaga
    40381 cggtacgccc atagcaaggg ccactgttga cgtccacttc gacgtacatc tcctgggttt
    40441 taatgccatc agggttaatc agataaccga tgcaggtggc gtcgtgcacc gggccgccag
    40501 ccaggccgta gttttcgaac tgcgttttga gagtgaagtt catgatgtcg ctgaacatct
    40561 ctccggcagg gccgcctgcc ctttccattc gtgcaattac gtccggggtg caaacggtct
    40621 ggttggtaag atcgaggccc atcatcacta atggaacgcc ggaggtgaac accacgcgtg
    40681 cggcttccgg gtcggcaaag atattgaact cggcagatgg ggtgaagttg cctgtaccgt
    40741 aagcgccgcc cattagcaca atttcacgga ttttgggcag gattgcgggt tgcatacgca
    40801 ttgccaccgc gatatttgaa agcggaccaa ccggcaccag agtgatatcg ccatcgcttg
    40861 ccataagggt atcaatgatg tatttcaccg cgtgggtact ttctgcctgg cgggtcagcg
    40921 gctcgaatac cgggccatcc agtccggttt caccgtgaat attatcggca acgatttgtt
    40981 gacgcataat gggttgcggc atccccgcat aaaccggaac attaatctcc agtttctggc
    41041 aaacattcag gccattaatt aatgttttat caagcgtctg attacccgca acaatggtga
    41101 tacctaataa atcaatcgcc ggatgtttcg ccgccatcat tatagcaata gcatcatcat
    41161 gacccggatc acaatccaga ataatttttc tcttttccat tgtttatttc ctctgtttcc
    41221 agttgcgtta ttttttctac agcaacgaaa tttcgatcgg gccgatgatt gaatcttaac
    41281 aacagcgtac gaatgctaaa tatgagaaat ctcatagcgg ataaacatcg tgaaagaaat
    41341 ccacaataat gatcttaagc agcaattgat gagtgaatcc gcgtttaagg attgcttttt
    41401 aacggatgtt tcagccgata cgcggctgtt tcatttttta gcgcgtgact acattgtgca
    41461 ggaagggcaa cagccgtcct ggctgtttta cctgacgcga ggccgcgcca ggctttacgc
    41521 cacgctagct aatggtcgcg tgtcgctgct cgatttcttt gccgccccct gttttattgg
    41581 cgagattgag ttaatcgaca aagaccatga accgcgtgcg gtgcaggcta ttgaagagtg
    41641 ttggtgcctt gcgctcccta tgaaacatta ccgcccgctg ttattaaacg acacgctatt
    41701 tttacgaaaa ctctgcgtca ccttaagtca taaaaattat cgtaatattg tttctttaac
    41761 tcagaatcaa tcatttccgt tagtgaatcg cctggcagca tttatattac tctcgcagga
    41821 aggtgatctt tatcacgaaa agcatacgca agcggcagag tatttaggcg tttcttatcg
    41881 acatctttta tatgttctcg cgcaattcat tcacgacggt ttattaatta aaagcaagaa
    41941 agggtatcta attaaaaaca gaaagcagtt gtcaggactg gcgctggaga tggacccgga
    42001 gaataaattc tccgggatga tgcagtaaaa attattggca atagcacgat tgccggatgc
    42061 aacgcttaat aaggcgtaca tccggcaatg aaactcaagc taaaccaata aagaacccgg
    42121 caatggtcgc gctcatcagg ttggaaagcg tcgccgccgc cagcgcccgt aaaccaagct
    42181 gggcgatttc cggcgcgcgt tttggcgaaa tagccgaaaa cgctccaacg acaacaccga
    42241 tagaaccaaa gtttgcaaac ccacagaggg cgaacgagat aatggcaatg gtcttgacat
    42301 ccagcgtgcc gctcgtttgc agatagggcg agaaattgag ataagcaaca aactcattaa
    42361 tcgccagttt ctgcccaatc aaacttccgg caagatttgc atcactccag tccaccccca
    42421 taatccatgc caacggggcc agcacatagc cgaaaatgct ttccagagag acattggcaa
    42481 aaccaaacca gccgccaact ccaccaataa tgccgttgat cagcgcaatg atcgctacaa
    42541 atgccattac caccgtcgcc acaccggcgg cgattttcag cccggtcatt gcaccgttcg
    42601 ccgcagcttc aataatgctt tttggcggtg tttcgctaaa cgacagattt tcaaacgtaa
    42661 cttgcgaagg ttccgtcgcc ggactaagaa tacgtgcaaa caggataccg ccaggaatag
    42721 ccatcagcga agcggctaac aaatagtcaa tcggcacccc cattccggca tagccaatca
    42781 tcatcgaacc cgcaatagac gccatgccgc tacaaatagc cgtaaatagc tcgttgcgat
    42841 tcatgcgatc aataaaaggt ttaacgatcg ccgggatctc attttgcccg aggaaaatag
    42901 tggtgactgc gacaaaagat tcgattttgc tgatgttgag ggctttctga aaaatactgc
    42961 caagaatacg aatcagcagc cccattacgc caatgtagta cagcaagctg ataagcgcgg
    43021 taacgaaaat aatcgccggg agtacgcgaa aggcgaagat aaagcccgca ccgtcaaaca
    43081 gaacatccat tttgggaccg accagcgaac caaaaataaa ggcacttcct gcgtcactgt
    43141 aggacatcac tttatggacg cctaatgccg cctgctctac tgcccatttt cccgggggaa
    43201 aatagagcat gatgccacca atggcaattt gcagcagcag cgcagcgcca accgtgcgca
    43261 agctgatgct ctttttattt accgacaaca caaaggcaat tgccagcaat accaccatcc
    43321 ccgcaacact tctcattata tccataatga ttttcccttc atgccagtaa acccggcgtc
    43381 agcgccaggt tttggtatgt ttgatgagta cgggcgacgg ctttctgccc gccagaaatt
    43441 aacccacgag acgctgatat tctttggcaa tttcgctcgc cagaatggcg ttattaaaga
    43501 ccagttgaat attggatttc aggctatcac cgccggttag ctccgcaaca cgcgccagca
    43561 ggaacggtgt actctctttc ccaataacac cctgttcttc ggcttcagca acggcttgat
    43621 caattgctgc attgatggtt tcttctggca tcgcaaactg ttccgggatc gggttcgcta
    43681 ccaccaggcc gccgttcaaa ccggtttgcc acttcactgc catcgcgcga gcaatttcgc
    43741 gggcactgtc aagacgaatg ctgacttcaa atgggctggt tcggcagaaa aacgcaggca
    43801 gcgcttttgt ctgataacca attaacggta caccgaaggt ttctaaatat tctgttgtta
    43861 atcctaaatc gagaatagat tttgcccccg cacaaacaac agtgacattg gtatttgcca
    43921 gttcctgtaa atcggcagaa atatcgaagg tatgttccgc tccgcgatgc acaccaccaa
    43981 tacctccggt tgcaaaaact ttaatcccag ccagtgctgc aataatcatg gttgatgcaa
    44041 cggtagtcgc gccatttttc cctgcagcaa cgacaaaagg taaatcacga cgactaactt
    44101 tcgtcacgtt atgtccttca cggcccagca attcgatttc ttctttactt aagcccactt
    44161 tcatcacacc gccaataatg gcgatagtgg ctggtactgc gccctgttta cgaattgttt
    44221 cttctacttc aattgcggtt tgggcatttt gtgggaatgg cattccgtga gaaattatgg
    44281 tcgattccag cgcaacaacc ggttttttat tttttaaagc atcctgtact tcagcggata
    44341 tttgtaataa ttcaggggac agagttaatt cagacattct gtgttctcca ctaacgatct
    44401 aacattggca attgataatt cagggttgtt ggtatattca caggcaagtg ccattgacga
    44461 acatccctgc gcaaatcgaa cagaatcgat aaacggcata ccgtctaccc aacaggaggc
    44521 cagccccgcc atcatggcgt cgcctgcccc agtaacattg atgacattcg ttttaatagg
    44581 ggccgaccac ccactttcac cgttgatatc actgtagtaa acgccatccc ctcccatgct
    44641 cagcaccaag cggttcaggc cgtgttgatg aaaccaggcg gcgacctttg cgacatcttc
    44701 acgtccggaa agtgcaattc cgctcaacgt ttcggcttca agacggttag gttttaacgt
    44761 atggattttg ctcagatgat cgcggatttt gacgcatttc catgccgata ccggatcaat
    44821 aaatacaggc gtttccccgc tattttccag aacccagacc agcacctctt cactaaggtt
    44881 gcaatccgcg acaataacct ttgcaccgcg aataaactcc tgatgctggg caagaaattc
    44941 tgcagaaata cagtcactaa tactcatatc atttatcgcg acaagcattt ctcctgtatt
    45001 atcgagcagt gataaatagc tggatgtatt ctcacccgga acaattaagc atttatcgac
    45061 ataaacaccg gattgattag tttgcgccag taatgattgc ccataaaaat caccacctac
    45121 tgcacttaaa agccaggcat ttttccccag taacgccaga ttatgagcga tattccttcc
    45181 cacgccacct ggggtaaatt taatttttcc gggattcgaa tccgcataat tcaatggatg
    45241 atgtgaatac ccagccacat caatattcgc cgaaccaata attactatgt agtccttttc
    45301 gcgcatagca cgccccctgg caataaattg ccaacgaaga aattgggtaa aatagccatt
    45361 acagtcaata agactattag agcacgtgtt cagatttaaa cacatgttca taataagcat
    45421 atttaacgta atgtaaatcg atcagaaaaa acgaaatcgg ccagatatga aggcggtcac
    45481 aaagtttgtc tatttatgca gcaacaaaag agaaagttgt gatgtgtaac acacaatatg
    45541 gcaggcaaga aaaagggcag agatgtttct gccctccaga ttgttgacaa cgcgcgcttt
    45601 gttcatcctg gatgtggcgt aaacgcctta tccgacctac aaaagcgcaa aaaattcaat
    45661 atattgcagg gttcatgtag gtctgataag catagcgcat caggcaattt tgcgtttgtc
    45721 atcatcgggg cagagatgtc tcatccccga taatgaagat aaattatgcg tttttttcta
    45781 ctatttgtgt ttccgggcgt ttcaggaagg cataggccaa acccgccacc agcgtaccgg
    45841 caataattgc taccaggtaa cccaataccg gcgtaatagc gccagggatc agcagaacaa
    45901 acagaccacc gtgtggtgcc atcagtttcg caccaatcgc cattgagatt gcgccagtca
    45961 gcgccccacc aacgatacag cacggtagca cacgcatcgg atcacgagca gcaaacggaa
    46021 ttgcaccttc cgaaataaag cacagaccaa gtaccagagc ggctttgcca ccttcctgct
    46081 gcgctttgtc gaatttgcga cgcgccacca ttgttgccag acccattgcc agcggtggca
    46141 ccatacctgc cgccataatc gccgccatcg ggccataggt ttgagtactc agcagaccca
    46201 caccgaatgc gtacgctgct ttgtttaccg gaccgcccat gtcagtacac atcatgccac
    46261 cgaggatcgc ccccagcaga accgcattcg cagtccccat ggtctgcagc cagtgagtca
    46321 acccatcgag aatgccagca accggtttac cgattaggta gatcatcgcc agaccgacca
    46381 ccagactgga aattagcgga atgatcagga tcggtttcag cgcctccata ctctgtggca
    46441 gtttcaattg cgtactgatt aacttcgcaa tgtaaccagc caggaagcca gcaattatac
    46501 cgccaataaa gccagaaccg gtgctgaccg ccagcatacc gccaatcaga cccggtgtga
    46561 ggcccggacg atcggcaatg gagaaggcaa tataacctgc cagtaccggc accatcagcg
    46621 caaaggctga accaccacca atctgcatca gcgccgctgc caacgtaccc ggctctttaa
    46681 acgcttcgat accaaaagca aaagaaagcg cgatacacag accacctgca acaaccatcg
    46741 gcagcatgta agagacgccc gtcagcaagt gacggtaagc gcctgcactc tctttcttac
    46801 cttcagtggt cgctgtttga gctttgcccg ctggttcata cggcgttgct tcagcaaccg
    46861 ctttatccag ttcctgcgca gttttcttca gcgccagacc ggtagaggtg cgatacatcg
    46921 gtttaccagc aaatttcgcc agatccactt cgatatctgc cgccacaatc accagatccg
    46981 ctgctgcgac ttcttcaggg gtgattgcat tgcccgcgcc aacagaacca cgggtttcaa
    47041 ctttcaccca ccagccacgt tttttcgctt cggtttcaat ggcttcagcc gccataaagg
    47101 tgtgtgctac gccagtcggg caagcagtca ccgcaactac gcgtttcgga ccgctggcag
    47161 caaccggtgc tgtcgcagcg accggcgcag tgtaaggttt cgcatggcct ttggcttcac
    47221 tcaggaacag ctcagggtgc gcaactgccc gggaaatatc acccagccag acatttttac
    47281 cgttcagcgc gctgtcattc gggatggaat caccgagaac aatcgccatt tctgcgtcgt
    47341 tcggattgtc gatgatttcc agttttgctt ttcgcgccgc cgcgcccagc agggtcttcg
    47401 ccatataggc gcgtgcctga ccgagattag cgtcaataat cagcagcgtt ttcattatgc
    47461 ctctcctgct gtcagttaaa aggttgtaag tcgacgcgcg ccatcattgc ggccaactgc
    47521 ggacgatcgg taatacccac attgctttga cttaccgcca gggctgcaac agctgtcgcc
    47581 agacgcagtg tgtgttcact ggattcacgc atcagcaagc cataaatcag gccaccaacc
    47641 atagaatccc ctgcgccaac ggtgcttacg acatcgactg acggtggttt ggcgatccat
    47701 tcgccggagg cattaaccca aagcgcgcct tcggcaccca gtgaaataac aacatgcgca
    47761 ataccttgtt cacgtagcgc atgtgcagct tcaatcacat ctttcatttc aggcagttta
    47821 cggcctgccc agatttccaa ctcgcggcgg ttaggtttca ccagccacgg tgccgctttc
    47881 aaacctgcta ctaacgcttc acggctacta tcaaagataa tgcaaggaca ctgactacgc
    47941 aggcgagtca tccagtcggt gaacgcttcc gggctgacgc ctgacggtaa gcttccgctg
    48001 acacagacca tatcgaactg accgagccag ctcagagaat cagtcacaaa gcgttcccag
    48061 tcggcgaggg tgacttcaaa acccgagaag ttgaagtcgg tcacttcgcc gtctttttcc
    48121 gtcagcttaa cgttaattcg ggtgcgcccc tgtacaacct ggaaacggtt ggcaatgccc
    48181 agctcgctga acagttgctg aaaaccatcc tgattgtctt tacccaggaa gccgccaacg
    48241 gtgacatcaa ttcccaggtc ttttaatact ttggccacgt tgatgccttt acccgccgca
    48301 tgcagaccgg tggttttcac caggttcact tcgccgcgtt caatttccgg gcagaaacca
    48361 acaaggtcat aagccggatt aagggtgatc gtagcaacac gtctgctcat tatgcgccct
    48421 ccccaagacc agcagcgata gcgtcgccaa tggctttcag cgcctgttca gcatctgcac
    48481 cctgggcggt aaagcgtagg cgatgacctt tcttaacgcc aagtgccaca actttcatca
    48541 gactacgtcc gtttgccggt ttgccggtgc catcaaggtt tgtcacggta atatcactgt
    48601 taaattgttt aatggtattg accagcatgg tacctggacg agcatgcagg ccgtgttcat
    48661 tgcgcaccac aaactccgcg cttaacacgt cgtcggtcgg cgcatcatcg ctggtcagca
    48721 gcgccagcaa cgttgccgca tccgctttca gcaagcggtc agctttattg tcgagcaata
    48781 aatcagcgag acgcttaaga accgcgatgg gctgatcgtc attcatcgcc acactcacca
    48841 gcatggctgc cgtttcgccg tccacatcaa aagcatttgc cgcacggctt accgcaatcg
    48901 cgctacgcag attgccttcg gcgctatcgc tcagccagat accctgtccg agattcagcg
    48961 gttgttcatt gatggctttg gtgacgaaag tggcgtcaac tgcccccacc tctttcagac
    49021 gcgcagcgtt cagcgcctga agagtcagca gatcgctggc gacgatatcc agcgtcagca
    49081 tttcgttgtc gagcttcagc tgctcactct gcttttcgcc catcagtaat gcgcgaagtt
    49141 cttctgctgt tgttgctgac ttcagttgtt cagcaacgga atcatcgctc agtacgtggg
    49201 tcagctggcg tagcaggccc agatgttcat ccgagctggc agcaataccg attgccacgt
    49261 acgctacctg accgtcaccc caggtgacgc cttccgggaa ctgaaatacc tgaacgccgg
    49321 ttttcagcac ctgatcgcgg gtgtcggtag tgccgtgtgg aatagcaata ccattgccga
    49381 ggaacgttga ggtttgctgt tcgcgcgcca gcatgccatt gacgtagcct tctgctacat
    49441 taccggcctg caccagcgcc gcagcgacct ggcgaatcgc ctcttctttg tctccggcct
    49501 tttcgcccgg atggatgtcc tgtacggata actggaacat agttctcctc tcttgctgaa
    49561 ttgaaacgat tcagcctcta tgagaaaaaa agcgccaacc tggcttaggg ttaaagacaa
    49621 gatcgcgctg aaacgtttca agaaagcata atacttctgt ttcagcacgc aaggaaagcc
    49681 gtaaagttat gagcaaaagt ttgatgtgct gcacaatttt ttgtcatttt tcctaattgc
    49741 tgatgggaaa atccgttatc agcaatttca tttcagcagc gtcagcagcc aatcagcaac
    49801 agtgaaatgg cattttgatt attcgtggcg cttttgattt cgctgtctgt ttattttctg
    49861 acaagcagcg taaactccgc gtcttcctct tccagtgatc gacctgcatg cataactccc
    49921 ccgcagtctc cagcgcgaaa tcgtttgacc tgacctcgac ggcgttttta atcgttgcct
    49981 ttctcaccgg tattgcgggt gctctgcaaa ccccgacact cagtattttc cttaccgatg
    50041 aagtacatgc ccgtccggcg atggtgggat tcttctttac cggcagcgct gtcattggga
    50101 ttctggtaag tcagtttctc gctgggcgct ctgataagcg cggcgatcgc aaatcgctga
    50161 ttgtcttttg ctgcctgtta ggcgtgctgg cctgcaccct ttttgcctgg aatcgcaact
    50221 actttgtttt gctattcgtt ggcgtctttc ttagcagctt tggctcgacc gctaacccgc
    50281 aaatgtttgc ccttgcccgt gaacatgccg acaaaaccgg acgtgaggcg gtgatgttca
    50341 gctctttttt acgcgctcag gtttcactgg catgggtcat tggcccaccg ctggcttatg
    50401 cgttagcgat gggtttcagc tttacggtaa tgtatctgag cgcagcggta gcgtttattg
    50461 tttgcggcgt gatggtgtgg ctgtttttac cgtcgatgca aaaagagctt ccgctggcga
    50521 ccggcacggt tgaagcgccg cgccgtaacc gtcgcgatac gctactgctg tttgtcattt
    50581 gtacattgat gtggggctcg aacagcctgt acatcatcaa catgccgcta tttattatca
    50641 acgaactgca tcttcccgag aaactggccg atgtgatgat ggggaccgcc gccgggctgg
    50701 aaatcccgac gatgttgatt gccggatatt tcgccaaacg tctgggtaag cgtttcttaa
    50761 tgcgcgttgc tgccgtgggt ggcgtctgtt tttacgcagg aatgctgatg gcgcattcac
    50821 ctgtcattct gttgggcttg cagctgctaa atgctatttt tattggcatt ctgggcggca
    50881 tcgggatgct ctattttcag gatctgatgc ccggtcaggc gggttcagcc accacgctct
    50941 ataccaacac ttcgcgcgtg ggctggatca tcgcaggatc agtggcgggc atcgtcgccg
    51001 aaatctggaa ttatcacgct gtgttctggt ttgcgatggt gatgattatc gccactctgt
    51061 tttgcttact gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc
    51121 aggtcatggc ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac
    51181 agactttcgg tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa
    51241 tgcagggcgt attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag
    51301 gggcagtgca acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta
    51361 accaccggat tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata
    51421 ccacttgccc gaaaggcccg tcgcgagtac tttgtcgcga tatttttgac attttcgact
    51481 acaggaattt ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca
    51541 acggcaaact gctgctggtt aaggatattg atattcagtc gcccactgcc cgcggtgccg
    51601 ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt
    51661 tcaaaggtga tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct
    51721 atgtcgatgg caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca
    51781 ccaaagatca gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc
    51841 aggtgctgac ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg
    51901 aaatcgttga aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg
    51961 cgacattgag cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa
    52021 ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg
    52081 cagaaaaggg atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg
    52141 cgcttcctga acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc
    52201 atcgcattaa caatccagac caatttcaat tgctgtcata taactttaca ctgtcattgt
    52261 taatttatcg ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc
    52321 atcatgtaca cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg
    52381 gctttggcgc ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc
    52441 agggcggcga ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc
    52501 agctccgcgc acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg
    52561 tgataattgt cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa
    52621 ttattgagaa attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag
    52681 gctattgtat tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg
    52741 atctacaaac ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac
    52801 gccgccgtga gcgcggcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca
    52861 atggccatgt ggtgaaaaac gcggtgctgg gaatggcaga aaaatgttcg ccagaactcg
    52921 ccgggtggat aaaaaaacac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg
    52981 ctgcaaccga cgaatcactg gcagaaatca gccagcatct gggggtaaat gatccctgcg
    53041 cgattagctg cgaaccgttt atccagtggg tggttgaaga taacttcatc gctgggcgtc
    53101 ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga
    53161 aactgcgtat gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat
    53221 tcgtccatat cagtgattgc atgcaggatc gcgtatttcg ccatgctgcc agaacattaa
    53281 tgctggatga gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg
    53341 ataagttaat tgcacgtttt gctaatccgt cgctgaaaca taagacctgg caaatcgcga
    53401 tggatggcag ccagaaatta ccgcaacgta tgctggcagg tattcgcata catctggggc
    53461 gtgaaacgga ctggtcgttg ctggcattag gcgttgctgg ctggatgcgt tacgtcagcg
    53521 gcgttgatga tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg
    53581 aacttgttgc gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag
    53641 ttttcggtga tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc
    53701 aacaaatcgt acaattcggc gcacatcagg cgctattaaa caccctcaaa atttaacgat
    53761 ttctgcggtt aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgcct
    53821 tttttagcca ggattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca
    53881 ggaccaacct catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt
    53941 tagcccataa agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg
    54001 gaattgatgg tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct
    54061 gcatgtgctg cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg
    54121 gaaaaccaga ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagatcctcg
    54181 atctgttaac cgcaccagtc tatgaactgt ggatagatct gcgcgccacc ttgtgcattc
    54241 tcgatccacg cctgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc
    54301 tggctgccgc agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc
    54361 aagcgctaca gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc
    54421 atgggaaagt tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg
    54481 ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc
    54541 cagagcatca acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct
    54601 ggatattcga cgctgatacg gtattcgaca ccattggcat tctggaatgg gcgcgacttg
    54661 caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc
    54721 gtcagggcga tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg
    54781 agctgatttc cagcagcgaa gctgactgga atgctttgca gagcgcgctg ttgaagcttc
    54841 gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat
    54901 gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc ggcctcgcgc tgtttctttc
    54961 ctggtatatt cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt
    55021 ctttaatcag aaactggtcg aaagtaaggc ctttttgtgg ctggttgcat tgaccaacaa
    55081 tcgcgctttc gacggttgtt ctctgctggc gatgggaatg ttgatgctga gtttctggct
    55141 gaaagaaaac gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac
    55201 tgcagtggta ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac
    55261 attgactttt accgatatta accgcgtcag cgaactgctc tctgttccca cgaaagatgc
    55321 ctcacgagat agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat
    55381 gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt attatttttg tggtttttgc
    55441 atttcccaga gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac
    55501 cgtgatattg atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac
    55561 cttttttgac aaatcactac cgggaaaaaa caaacatttc caaaacaaat aactcacagt
    55621 aattaacatc atcaggctta tttttatagt gaggataatc ctgatgatgc gcaccgtgct
    55681 ttcatccatc gcgcgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac
    55741 tcaatcgtac tttaattgac caatcccgct tattaacttt ctgtatcact ttttcttata
    55801 aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg ggtaatctag aactcgtttt
    55861 gcctcggcgg tagattatcc tcacagcata taattttgtg cgttagtcca cagatttggc
    55921 cttaaggaat tgtttcaaca tgcccaggta attagtctcg tgtcgcttgg cattttttta
    55981 taacgatatt tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc
    56041 gattttgaga tatatcttgc gcgggatccc cgcgattgca gtagcggttc tgctttctgc
    56101 atgtagtgca aataacaccg caaagaatat gcatcctgag acacgtgcag tgggtagtga
    56161 aacatcatca ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt
    56221 aaaatcgcga attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg
    56281 cagcactaaa aaaggtatcg attgttctgg ttttgtacaa cgtacattcc gtgagcaatt
    56341 tggcttagaa cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtttcccg
    56401 cagtaatttg cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt
    56461 cggtatttat atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat
    56521 ttccagcatg aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag
    56581 ccgcagctaa taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag
    56641 cactgcttag gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct
    56701 tgatccaggc tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat
    56761 taacggaatc atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat
    56821 tgtagcaggc gtgatgattg cgatactggt tagctgcctt cagtttttag tggcctggca
    56881 taagcacgaa gtcaaatacg acacactgat tatcgacgta caaaagtatc tcgataccta
    56941 ttttgccgac ctgaaatcca ctactgaccg gcttcagccg ctgaccttag atacctgcca
    57001 gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt
    57061 gctggtgaaa gataaaaaaa cattctgttc atctgcgact ggtgagatgg acattccact
    57121 caatgaattg attccggcgc tcaacattaa taaaaacgtc gatatggcga tcttacccgg
    57181 cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa
    57241 aaatagcggt gtgtttgccg ctctgaatct caacctgacg ccttctctct tttatagttc
    57301 acggcaggaa gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt
    57361 ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg ccggtccgtg aaactaaaat
    57421 tgcgggcatt cctctgaccg ttcggcttta tgcggatgac tggacatgga acgatgtgtg
    57481 gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt ggcctgctct gctattacct
    57541 gatgagcgta cgtatgcgcc ccggcagaga aatcatgacc gccatcaagc gcgaacaatt
    57601 ttacgtggcg tatcaaccgg tggtggatac acaagctttg cgagtaacgg gcctggaagt
    57661 actgctacgc tggcggcatc ctgtcgcggg agaaattccc ccggatgcct tcattaactt
    57721 tgccgaatcg caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg
    57781 cgatgccgca gaattagaaa aagtgctgcc agtaggcgtc aaatttggta ttaacattgc
    57841 gccggaccat ctgcacagcg aaagctttaa agcagatatc cagaaactgc tcacttccct
    57901 gcccgcacac catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca
    57961 agaagccaca caactcttcg cctggctgca ctcggtcggc gtagaaattg ctattgatga
    58021 cttcggcacc gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa
    58081 aattgaccgt ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga
    58141 cgcggtgctg acgctggcga aacgcctcaa tatgctgacg gttgctgaag gggtcgaaac
    58201 gccggaacag gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat
    58261 tagtcgccct ttaccgctgg acgattttgt ccgctggctg aagaaaccgt atacgccgca
    58321 gtggtaaggt gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta
    58381 atgatgagta aaaggaaatc cattgcagat gattgtgcgc atactgctgc tgtttatcgc
    58441 tctgttcacc tttggtgtgc aggcgcaggc tatcaaggaa agctatgcct ttgccgtgct
    58501 gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc
    58561 aaaaggtggg cagataacgt tgtcagccct cggcaccttc gataatttca accgctatgc
    58621 actgcgcggc aacccgggcg cacgcaccga gcagctgtac gacacgctat ttacgacttc
    58681 cgatgacgaa ccaggcagtt attacccgct gattgctgaa agcgcacgct atgctgacga
    58741 ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttcgcccat
    58801 tactgcccgc gatgtagagt ttacttttca aaaatttatg accgaaggcg tgccgcaatt
    58861 tcgtctggtc tacaaaggca ccaccgtcaa agccattgca ccgttaaccg tgcgcattga
    58921 gttagctaaa cccagcaaag aagatatgct gagtctgttt tcgctgccga tatttccaga
    58981 aaagtactgg aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg
    59041 tccgtaccgc gttacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga
    59101 ttactgggca gcaaacttac cggtaaaccg tggacgctgg aatttcgaca ccattcgcta
    59161 cgattattac ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt
    59221 gcgtatggaa aacgacgcta aaaactgggc cacgcgttat accggtaaaa atttcgataa
    59281 aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc
    59341 gtttaatatc caacgtccgg tattcagcga tcgccgggtc cgggaagcaa tcactctcgc
    59401 ctttgacttt gaatggatga acaaggcgtt gttttacaac gcctggagtc gcacgaacag
    59461 ttattttcag aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct
    59521 tctggcacca atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc
    59581 ggtatctaaa ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa
    59641 cgaaacgggc tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccgct
    59701 cagctttgaa ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca
    59761 cagcctgcaa cggctgggta tcaatatgga cattcgcaag gtggataact cgcaaatcac
    59821 taaccgcatg cgcagtcgcg actatgacat gatgccgcgc ctatggcggg cgatgccgtg
    59881 gcccagttcc gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc
    59941 ccccggcgtg caaagcccgg ttatcgactc gctgatcaac caaattattg ccgcgcaggg
    60001 aaataaagaa aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta
    60061 ttacatgctg ccaatgtggt acatggcgga agaccgtctc gcctggtggg ataaattctc
    60121 ccaacccgct gtacgccctg tttacagcct gggtatcgat acctggtggt atgacgttaa
    60181 taaagcggcc aaactgccgt cagccagaca acagggagag tagatgggcg cttacctgat
    60241 tcgccgtctg ttgctggtga tcccaacgtt atgggctatt atcactatca actttttcat
    60301 cgtgcaaatt gcgcctggcg gtccggtcga tcaggccatc gccgccattg agtttggtaa
    60361 tgccggagta ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg
    60421 tgtcggcaat atcagcgaca gtaattaccg tggcggacgc ggattagacc cagaagtgat
    60481 cgctgagatc actcatcgct acggtttcga taagccgatc cacgaacgtt acttcaaaat
    60541 gctctgggac tacatccgct ttgattttgg tgatagcctg tttcgcagcg cctcggtgct
    60601 gacgctgatt aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat
    60661 tatctatctg gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagtcgctt
    60721 tgacgtctgg agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc
    60781 catcctgctg attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg
    60841 cctggtttcc gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg
    60901 gcatatcacg ctaccggtgc tagcgacagt gattggtggc tttgcggcgc tgaccatgct
    60961 gaccaaaaac tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa
    61021 aggggtaagt gagaaaaaca ttctctggaa acatgtgttc cgcaacgcca tgctgctggt
    61081 gattgccggt tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga
    61141 ggtgatgttt tcactcaatg gtcttggctt actgggctac gaagcgaccg tctcgcgcga
    61201 ttatcctgta atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat
    61261 tgtcagtgat atcagctata cgctggttga tccgcgtata gattttgagg gacgttaatg
    61321 tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt ttcgtcataa ccgtcgcggc
    61381 tactggtcgt tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc
    61441 gctaacgata aaccgttgct ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa
    61501 aactacagcg aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg
    61561 tggctgaaac aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt
    61621 gctaccagta tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac
    61681 tggctgggaa cggatgccaa cggcggcgat gtgctggcac gcattctcta tggcacgcgg
    61741 atctcggttc tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg
    61801 ggggcgctac aaggctatta cggcggtaaa gtcgatctct ggggacaacg ctttattgaa
    61861 gtatggtcgg ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct
    61921 aacttctggt ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg
    61981 gtgcgggcgg agtttttacg tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt
    62041 ggcgtcagcg atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc
    62101 ctcacctttt taccgtttat tttatgtagt tcgataacca ccctgacctc gctcgatttc
    62161 ctcggcttcg gtctaccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa
    62221 aataaccttc aggccccgtg gcttgggatc accgccttct tgtcggtggc gatattattg
    62281 tctttgctga tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg
    62341 tagcatgacg caaactctgt tagcgattga aaatttgtcg gtgggttttc gccatcagca
    62401 aaccgtacgt acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc
    62461 gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct
    62521 cccttccccg ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct
    62581 tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca
    62641 ggaaccgatg gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct
    62701 ttcactccac cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta actgccttga
    62761 tcgcgtcggt atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg
    62821 cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat
    62881 tgccgatgaa ccgaccaccg cactggacgt ctctgtccag gcgcagattt tacagctgtt
    62941 gcgcgaactg caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat
    63001 tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa
    63061 taacgccgct acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag
    63121 tgaaccgtca ggcgatccgg taccgttgcc agaacctgcc tcaacgttgc tggatgttga
    63181 acagcttcag gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa
    63241 tgtggtggtg aaaaacatca gttttacgct acgggcgggt gaaacactgg gtttagtggg
    63301 cgagtctggt tccgggaaaa gcacgacggg actggcgctg ctgcgactga ttacctctca
    63361 gggcagcatc gtctttgacg gtcagccact gcaagattta aatcgccgcc agctgttacc
    63421 tactcgtcat cgtattcagg tggtatttca ggatccaaac tcctcgctca acccacgact
    63481 caacgttttg cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc
    63541 acaacgcgaa caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg
    63601 ccaccgttat ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc
    63661 attaattctt aagccctcac tgatcatact tgatgaaccg acatcatcac tcgacaaaac
    63721 ggtacaggcg caaatattga cgctattgaa atcattgcaa caaaagcatc aactggccta
    63781 tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact
    63841 gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgccg caccgcagca
    63901 ggagtatacg cgtcagctac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa
    63961 agggttcggc aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct
    64021 ggcgatttac aaacaggcac ggctcacctt cacactccag caccgaacat ggaacttcga
    64081 tgtcgcttaa cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc
    64141 taagcaattt aagcgccacc aggctgttgt caccgcacgg tccattttgc ggaatcggtt
    64201 caaccttcga acctgcgaaa ccagcagacc agcgaaagtt ctgcggtaga cgatggacga
    64261 ttgagagttg taatgaagtc acttattttc cccggaagca catttacttc acaatttatt
    64321 tacatcaatt ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc
    64381 ctcgcctgca tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt
    64441 gcggggctat atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg
    64501 actttttaca taaattgatt ttacataaaa taaacatata tcgggggaat gatagatttg
    64561 tggttgacat gcatcaacaa aggaagcctt ttagcttcct cgttgtgcaa tagatcaccg
    64621 ttttttcggt cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat
    64681 tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat
    64741 tgcccctatg ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg
    64801 aaactcatca agaatgaccg ccatcgcatt ggatgacacc atagacacgc agcccacaaa
    64861 cgccgcaacg ccaaccacca gcgaccagaa tcccagcccc agcagcgcac tgatgaccat
    64921 ccacgctgcc ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat
    64981 gcggcggacg aagcggctgt taaagatggt catcacgaac agaaaaacaa tgtttagcgc
    65041 aaagtaataa ccaaagtttt ccggtgcaac gtggttgatt tcaatgtaga caaacggtcc
    65101 ggcgctcagg aacgagaaca ttccggcaaa gctgaaaccg ctggccagca tataactcag
    65161 gacacgctta tgacggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaaagg
    65221 ctggcgagga tccagaagat gtaatgccag ctcagccaca ccagcaccca gccgccaact
    65281 atcggtgcca tcagcggtgc aatggtcgtc accagcatga caaacgacat catccgcgag
    65341 aactcttctt tcgggtaaat atcacgcatc aaagcgttga taaccacgct ggccgcagcc
    65401 gcagccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtttg tgccaacgca
    65461 cacgccaccg cggcggcggc aaacaccagc gtaccgccaa gaaccaccgg cttacgcccg
    65521 aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag aatataagta
    65581 ctcagggtca tctgcgtact gcccgccggt acgccaaact gcgctgaaat taccggtagc
    65641 gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc aaggataaaa
    65701 acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa gtcattgaaa
    65761 agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc gggggctaaa
    65821 tcagcatcca gcgtaatgcc gccaatacgt tcacgatgca gctcaaccac atggttaccc
    65881 acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt cagacgaacc
    65941 tgcgttgggg taatcacttc cagcaccgcc ggcttagtga gatctttttc gttatgcagc
    66001 tgcacgcctt tagcaaattg ttctgccgta tcgtcagcta cgggtgatac cagcgtcacc
    66061 agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca ctgaccatca
    66121 tctgtcatta gtaccagacc ggtggtatcg atatccagcc gccctgcggc gtgcagtttc
    66181 cacgctaccg gttcatctag aaaatagagc accgttgggt gatcagggtc gtccgtggag
    66241 caaacataac cctgaggctt attgagcatg aagtaacgtg gaccgtgttg ctgcgccagc
    66301 gggttgccat cgtaagcgac atcatgttca ggaagcagtt tgaacgctgc attacggacg
    66361 atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat agcacggcta
    66421 acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt gcctgtaaaa
    66481 aacggaggtc gggtaatgac ccgaatatct gaactgttgt ctgcccagta tagcggtcta
    66541 ataacatccc tcaagggaaa aagatccgtg gcatactatt agcagaataa tctacctacg
    66601 cgagaccatg atttttacac ttcgcccata tcagcaagaa gccgtggatg ccacgctcaa
    66661 ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg cccaccggtg caggtaaaag
    66721 cctggtgata gcggaactgg cacggctggc tcgtggtcgc gtactggtgc tggcacacgt
    66781 taaagaactg gtggcgcaaa accatgcaaa gtatcaggcg ctggggctgg aagccgatat
    66841 ttttgccgcc gggctgaagc gtaaagagag ccacggtaaa gtggtatttg gcagtgtgca
    66901 gtcggtcgcc cgtaatattg atgcctttca gggtgaattt tcgctgttga ttgtcgatga
    66961 atgtcaccgt attggtgacg atgaagagag ccagtatcag caaatcctca ctcacctgac
    67021 aaaagtgaat ccccacttac gcctgctggg gctgactgcc acgccttttc gattgggcaa
    67081 aggttggatc taccagttcc attatcacgg catggtacgc agcgatgaga aagccctttt
    67141 ccgtgactgc atttatgagc tgcccctgcg ttatatgatt aaacacagct atctgacgcc
    67201 gccagaacga ctggatatgc cagtagtgca atatgatttc agccgcttgc aggcacagag
    67261 caacgggctg ttcagcgaag ccgatctcaa ccgtgagctg aaaaaacagc aacgtattac
    67321 cccgcacatc atcagccaga ttatggagtt cgccgaaaag cgtaaaggag tgatgatttt
    67381 cgccgcgacg gtcgaacacg caaaagagat tgtgggatta ctgcctgccg aagatgcagc
    67441 actgattact ggcgacaccc ccggcgctga gcgcgatgtg ttaattgaaa attttaaagc
    67501 ccagcgtttt cgctatctgg tcaacgtcgc ggtactgacc accggatttg acgccccgca
    67561 cgtcgatctt atcgccattc tgcgccctac cgaatcagtg agtctttacc aacaaattgt
    67621 cgggcgcggt ctgcgtctcg ctccgggcaa gactgattgt ttaattcttg attatgcggg
    67681 taatcctcac gatctctacg cgccggaagt tggtacaccg aaaggcaaaa gtgacaacgt
    67741 tccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg ggaaaacgac
    67801 cgccgacggg acattgattg aacactttgg tcgccgctgt cagggatggt ttgaagatga
    67861 cgacggtcat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc cgcaatgtaa
    67921 cgcagaaaat gatattgccg ctcgccgctg ccgcgaatgt gacacggtac tggttgatcc
    67981 ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg ctggtattac gctgtagcgg
    68041 catgtctttg cagcatgggc acgacgagaa aggcgaatgg ttgaaaatca cctattacga
    68101 tgaagacggt gcggatgtga gtgagcgttt ccgtctgcaa acacctgccc agcgtaccgc
    68161 cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatcccgc tgcgctggat
    68221 caccgccgcc gatatcctcg cccagcaagc cttattgcga cccccggatt ttgtcgtcgc
    68281 ccgcatgaaa ggccagtact ggcaggtgcg tgaaaaagtg ttcgattacg aaggtcgttt
    68341 tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata aggcgattga
    68401 gtatagaatc tcacccgctt ttgcatacgc aaagcagatc acttacctgt tgctgggtcg
    68461 cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca gaagtacgta
    68521 aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc ccggcaatca
    68581 tctacggtgg caaagaagcg ccgctggcta tcgagctgga tcacgacaaa gtcatgaaca
    68641 tgcaagctaa agctgaattc tacagcgaag ttctgaccat cgttgttgac ggtaaagaaa
    68701 tcaaagttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg cagcacatcg
    68761 acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctcccg cggggttttt
    68821 tgtatctgca gattatgcct gattacggta ttgctatttt ttgcaggcca gataaggcgt
    68881 tcacgccgca tctggcattc agaacacaaa accgattaat tgccgccaga cgtccggcgc
    68941 tgcaattggt cgcgcaaatt cggcggcgtg cctttaatgg tcagggtatc ggtcgccggc
    69001 tcccagaaga tgcgttcacc taacagcatc gcatcaaagt taatagtcaa gccaccgccg
    69061 ctaccggcaa acttcgtcaa ctgacgcagt gtgctccggt ctgccgggaa gctctcttcc
    69121 agttcgtagc ctttttcagc cgcaaactcc gtgaagctca cttcgcttac gcctgccagc
    69181 tctttcgaca gcgattccag ttcgatctct tcgcctgcct gcagctgctc attacagtag
    69241 ctataaacct gctggcgcac gttctgtcgc tctgctttat ccagttgtgc ttcggcagtg
    69301 aaatcatcaa ccgcttgcaa cagcccacgg ttttgtgctt tcgtgttcaa tccttcgctg
    69361 gccccgagga aatccataaa gaaatccgcc actttgcgcc ctactcgccc tttcaggaaa
    69421 gtgagatagc gggtggactc tggattggtt tcccattcgg ttaaatcgat acgcgcaaca
    69481 atatccgcat gattgatatc aagataatgg gttgggttga tgtcgagatt ttcgttaaca
    69541 cgcatactgc tcaggttgct cagaactgcc accagcagat actccaccgc cagataacgg
    69601 tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa ttcgtcacgc
    69661 aggcgaccag ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg acgctgtaag
    69721 cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt atttttggcg
    69781 ctatagaccc gatgcagttc tgccaccatc tcaacgacgg tttctgtcgg ttccagcaat
    69841 gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg gtgcagagca
    69901 atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg tattcaacca
    69961 ccgccggtta agcgacgcaa taaaagatgg cggtcatttt acagaaggca gcgtaacgag
    70021 aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata tgttgccctt
    70081 tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtga tgaacaggtt
    70141 gaacaattgc tcgcagagct gctcaacgta ctggaaaaac ataaggctcc gaccgacctt
    70201 tccctgatgg tgttaggcaa catggtcact aaccttatca acaccagcat tgccccggcc
    70261 caacgccagg caattgccaa ctcttttgcc cgcgccttac agtcctctat caacgaagac
    70321 aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc gctaccgtga
    70381 aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata ttctgctttc
    70441 gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgacaacgc ttgctggtcg
    70501 catttattcc tacgtaagca ttatcggcca tttcagcttc ctggtgttcg ccacctactt
    70561 gctgatcctc ttcccgctga cctttatcgt cggctcccag aggctgatga ggtttttgtc
    70621 cgtcattctg gcaacggcgg gaatgacgct attactgatc gatagcgaag tctttactcg
    70681 tttccatctc catcttaatc ccatcgtctg gcagttggtt atcaacccag acgaaaatga
    70741 gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat tgcttgaact
    70801 ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc gacgcttcgc
    70861 gcgcccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg tgtatatctg
    70921 ggccgatgcc aacttctatc gcccgatcac catgcagcgc gctaacctgc cgctttcgta
    70981 cccgatgacg gcgcgacgtt ttcttgagaa gcatggtctg cttgatgcgc aggagtatca
    71041 acgccgtctt attgagcaag gtaatccaga cgccgtttcc gttcagtatc cgttaagcga
    71101 actgcgctat cgcgatatgg gcaccgggca gaatgtgctg ttgattactg tcgatggcct
    71161 gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg agcaaaatat
    71221 ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct ttggcctgtt
    71281 ctatggtatc tcgccgagct atatggacgg cattctgtcg acccgtacgc ctgcggcatt
    71341 aattactgcg cttaatcagc aaggctatca gctggggtta ttctcatcag atggctttac
    71401 cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagcg tacgcaccca
    71461 atccgacgag cagaccgcca cgcagtggat caactggctg ggccactacg cacaagaaga
    71521 taaccgctgg ttctcgtggg tttctttcaa tggcactaac attgacgaca gcaatcagca
    71581 ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga tcaaccgcgt
    71641 gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gttgtgatta tcactgccgg
    71701 tcggggtatt ccgctgagcg aagacgaaga aacctttgac tggtcccacg gtcatctgca
    71761 ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg cgctgactga
    71821 tcataccgat ctgatgacga cgctgatgca acgcctgcta catgtcagca cacctgccag
    71881 cgaatattcg caaggtcagg atttgttcaa ccctcaacgc cgtcattact gggttactgc
    71941 agcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc tgaacaataa
    72001 cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa aaccacagtt
    72061 aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt atcgctaact gattaattat
    72121 aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt attagcagcc
    72181 acgagtcggc acgtagcgca gcctggtagc gcaccgtcat ggggtgtcgg gggtcggagg
    72241 ttcaaatcct ctcgtgccga ccaaaaatcc caagaaaaaa ccaaccttta cggttggttt
    72301 ttttatatct gcaattaatt tgataaacag accgtgacac atcacagcct gtttattttc
    72361 tgttatcaga acgtccagac cactcccgcc tgagtattcc acggcgattc tacccctgcg
    72421 ccattgccat agctgactga caaatgaccg cttaacgtcg gggtaaacga tgaccttatc
    72481 ccagcctgat aaacgccacg ggttccagcc acatcattat taaatttgcc atcgtcatta
    72541 actttcacat ggttagaatc ggcgtattcc tgacgcacgg cggctttcag ccagggttcc
    72601 aacgtcgtac cgttttgcag gtccatgtga tagcttaccg ccgttcccgc ttcagcgcgt
    72661 aatatccggg tatttcccac atccgcgcgc atgccgtttg ataacgtgta gtcctgacca
    72721 tctgtggtaa agccggtaaa ggcgagatag ggtctaacac tccacaatcc gtcaacccaa
    72781 cggaacccgc tctcaacatg agcacccgcg ccgttactat tgtaatcgcc aaacgctgtt
    72841 gccccattac tcatcttgcc atggatggtg ttggcaaaac ggtcaacttt caccacccca
    72901 tcaacatagg caccgttctg atgctcccaa ccggcataag cccccagggt gtagctatca
    72961 atattgcctt tgccgccgcg atcaaaacca atatcagaat gagagtaatc aaagaacaag
    73021 ccgcgaattg tgctgctttc ttcacgggag aaacggctat cgataccgag cgtcaggccc
    73081 gtcaatgttt gctcaaagcc agctcccgca tcagtggtca cgttgttgcg ggtgttaatt
    73141 gccgaactcc acatcgccgt atcgtaatta acgcctttta cgctaccaag acgctcacgc
    73201 acggtgtcca gttctgcatc aaataccagc ggttgtgctg ctgccatgtt cagcacatca
    73261 gtggttgaag gggtaatttg cgcgcgattc tctgcgaggc tccagctatg gttgccatta
    73321 tccagcaagg tatattcata cgtaccgata tcaacaacgc ctccggcatt gcccaacgtg
    73381 aatgcagcat caccgccgcc cgtcgttacc agtgtaaggc tatctcctgc tgccgggctg
    73441 gcccccgtgt ccgtcacgaa tattttgaaa tcacctgttg cctgaccgat gacgttgagc
    73501 tgatcgctct gatgattagc catatcggta cgcatataaa attgccgttt cccgacaggg
    73561 tattggtggt caacgtaatg aaattttccg cacttgccgt cacagatgaa cgagtcactg
    73621 gctcagccat cataatcaca tttgcgccgg acatcgcaag gttggctaag ttgaggcgag
    73681 tgttttggtt cgtcgtagtg atatcggcaa tgatggtcca tgcgctattt tcgagcgaaa
    73741 cgtccgcttg gctggtatcc acggcaccat gcgttctaaa agaagcgccc tcagaaacca
    73801 ctaccttacc caccaacata gcgggtgcat aatttatttt cggcttcatg acctcaagaa
    73861 tgccatcatt ccctctgact gacacgttaa ccattgtgcc agcccagaca ttagcgcggc
    73921 cattgttgat aaccatattt tcagagatgg ctttagagga atacgtgata cttccattct
    73981 gatctgatcg accgagatca tatacagcat ttgcctgcac tttcgtacca gtatcctttc
    74041 ccagcgattg catagtggca tgctcatcaa ggatagtgtc aatggcctga gtgtcctcca
    74101 taacaatgag cccggagcca tcatccagtt cataattttt tgacacacca tcctttatac
    74161 taaattggcc tttactgttt gtaccactca cttccagcgc attcgttgag acaattaatt
    74221 tcccgcctgc ttttttatca acgccagttg cagtcccgcc atccataacc tccagtaatc
    74281 ctcccttttc gatagcggca aagacggcaa tcccatcttt ttcaacaatt aagcgcccgc
    74341 ctttaatcgt gggatcattc gccgcg
//
LOCUS       DAETQN010000005        70301 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.005, whole genome shotgun sequence.
ACCESSION   DAETQN010000005 DAETQN010000000
VERSION     DAETQN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.005"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..673)
                     /locus_tag="K0W68_000361"
     CDS             complement(<1..673)
                     /locus_tag="K0W68_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HBI3072036.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="K0W68_000362"
     CDS             complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="K0W68_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HBI3072037.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="K0W68_000363"
     CDS             complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="K0W68_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBI3072038.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="K0W68_000364"
     CDS             complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="K0W68_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HBI3072039.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="K0W68_000365"
     CDS             complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="K0W68_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBI3072040.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="K0W68_000366"
     CDS             complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="K0W68_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HBI3072041.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="K0W68_000367"
     CDS             complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="K0W68_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBI3072042.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="K0W68_000368"
     CDS             complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="K0W68_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HBI3072043.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8399..9751)
                     /gene="hyfD"
                     /locus_tag="K0W68_000369"
     CDS             complement(8399..9751)
                     /gene="hyfD"
                     /locus_tag="K0W68_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HBI3072044.1"
                     /translation="MGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLV
                     IDRMSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQLSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPSVGFCVAALAITGVPPFNGFFSKFPLFAVGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(9727..9837)
                     /locus_tag="K0W68_000370"
     CDS             complement(9727..9837)
                     /locus_tag="K0W68_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HBI3072045.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="K0W68_000371"
     CDS             complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="K0W68_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBI3072046.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="K0W68_000372"
     CDS             complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="K0W68_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HBI3072047.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="K0W68_000373"
     CDS             complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="K0W68_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBI3072048.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="K0W68_000374"
     CDS             complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="K0W68_000374"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBI3072049.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14170..14742)
                     /locus_tag="K0W68_000375"
     CDS             complement(14170..14742)
                     /locus_tag="K0W68_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBI3072050.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14888..15766
                     /gene="dapA"
                     /locus_tag="K0W68_000376"
     CDS             14888..15766
                     /gene="dapA"
                     /locus_tag="K0W68_000376"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBI3072051.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15783..16817
                     /gene="bamC"
                     /locus_tag="K0W68_000377"
     CDS             15783..16817
                     /gene="bamC"
                     /locus_tag="K0W68_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBI3072052.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17030..17743
                     /gene="purC"
                     /locus_tag="K0W68_000378"
     CDS             17030..17743
                     /gene="purC"
                     /locus_tag="K0W68_000378"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBI3072053.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17911..18759
                     /locus_tag="K0W68_000379"
     CDS             17911..18759
                     /locus_tag="K0W68_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBI3072054.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18774..20789
                     /gene="tmcA"
                     /locus_tag="K0W68_000380"
     CDS             18774..20789
                     /gene="tmcA"
                     /locus_tag="K0W68_000380"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBI3072055.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20863..21561
                     /gene="ypfH"
                     /locus_tag="K0W68_000381"
     CDS             20863..21561
                     /gene="ypfH"
                     /locus_tag="K0W68_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBI3072056.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21671..21871)
                     /locus_tag="K0W68_000382"
     CDS             complement(21671..21871)
                     /locus_tag="K0W68_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBI3072057.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="K0W68_000383"
     CDS             complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="K0W68_000383"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBI3072058.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23030..23386)
                     /locus_tag="K0W68_000384"
     CDS             complement(23030..23386)
                     /locus_tag="K0W68_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBI3072059.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23768..23827
                     /gene="ypfM"
                     /locus_tag="K0W68_000385"
     CDS             23768..23827
                     /gene="ypfM"
                     /locus_tag="K0W68_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBI3072060.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="K0W68_000386"
     CDS             complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="K0W68_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBI3072061.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="K0W68_000387"
     CDS             complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="K0W68_000387"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HBI3072062.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSPPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29109..31088
                     /gene="aegA"
                     /locus_tag="K0W68_000388"
     CDS             29109..31088
                     /gene="aegA"
                     /locus_tag="K0W68_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBI3072063.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31156..31731
                     /gene="nudK"
                     /locus_tag="K0W68_000389"
     CDS             31156..31731
                     /gene="nudK"
                     /locus_tag="K0W68_000389"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBI3072064.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            31857..32900
                     /locus_tag="K0W68_000390"
     CDS             31857..32900
                     /locus_tag="K0W68_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBI3072065.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="K0W68_000391"
     CDS             complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="K0W68_000391"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBI3072066.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35019..35969)
                     /gene="tal"
                     /locus_tag="K0W68_000392"
     CDS             complement(35019..35969)
                     /gene="tal"
                     /locus_tag="K0W68_000392"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBI3072067.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36258..38537
                     /gene="maeB"
                     /locus_tag="K0W68_000393"
     CDS             36258..38537
                     /gene="maeB"
                     /locus_tag="K0W68_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBI3072068.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            38830..39165
                     /gene="eutS"
                     /locus_tag="K0W68_000394"
     CDS             38830..39165
                     /gene="eutS"
                     /locus_tag="K0W68_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HBI3072069.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            39178..39657
                     /gene="eutP"
                     /locus_tag="K0W68_000395"
     CDS             39178..39657
                     /gene="eutP"
                     /locus_tag="K0W68_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBI3072070.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            39632..40333
                     /gene="eutQ"
                     /locus_tag="K0W68_000396"
     CDS             39632..40333
                     /gene="eutQ"
                     /locus_tag="K0W68_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HBI3072071.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            40330..41133
                     /gene="eutT"
                     /locus_tag="K0W68_000397"
     CDS             40330..41133
                     /gene="eutT"
                     /locus_tag="K0W68_000397"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HBI3072072.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            41130..42146
                     /gene="pta"
                     /locus_tag="K0W68_000398"
     CDS             41130..42146
                     /gene="pta"
                     /locus_tag="K0W68_000398"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBI3072073.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            42185..42478
                     /gene="eutM"
                     /locus_tag="K0W68_000399"
     CDS             42185..42478
                     /gene="eutM"
                     /locus_tag="K0W68_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBI3072074.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            42585..42872
                     /gene="eutN"
                     /locus_tag="K0W68_000400"
     CDS             42585..42872
                     /gene="eutN"
                     /locus_tag="K0W68_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HBI3072075.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            42884..44287
                     /locus_tag="K0W68_000401"
     CDS             42884..44287
                     /locus_tag="K0W68_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HBI3072076.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            44298..45134
                     /gene="eutJ"
                     /locus_tag="K0W68_000402"
     CDS             44298..45134
                     /gene="eutJ"
                     /locus_tag="K0W68_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HBI3072077.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            45124..46311
                     /gene="eutG"
                     /locus_tag="K0W68_000403"
     CDS             45124..46311
                     /gene="eutG"
                     /locus_tag="K0W68_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HBI3072078.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            46628..47854
                     /gene="eutH"
                     /locus_tag="K0W68_000404"
     CDS             46628..47854
                     /gene="eutH"
                     /locus_tag="K0W68_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBI3072079.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            47851..49254
                     /gene="eutA"
                     /locus_tag="K0W68_000405"
     CDS             47851..49254
                     /gene="eutA"
                     /locus_tag="K0W68_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBI3072080.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            49266..50627
                     /gene="eutB"
                     /locus_tag="K0W68_000406"
                     /pseudo
     CDS             49266..50627
                     /gene="eutB"
                     /locus_tag="K0W68_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            50648..51535
                     /gene="eutC"
                     /locus_tag="K0W68_000407"
     CDS             50648..51535
                     /gene="eutC"
                     /locus_tag="K0W68_000407"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HBI3072081.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            51545..52204
                     /gene="eutL"
                     /locus_tag="K0W68_000408"
     CDS             51545..52204
                     /gene="eutL"
                     /locus_tag="K0W68_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBI3072082.1"
                     /translation="MPALDLIRPSVTAMRVIASVNSEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            52217..52717
                     /gene="eutK"
                     /locus_tag="K0W68_000409"
     CDS             52217..52717
                     /gene="eutK"
                     /locus_tag="K0W68_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBI3072083.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            52763..53815
                     /gene="eutR"
                     /locus_tag="K0W68_000410"
     CDS             52763..53815
                     /gene="eutR"
                     /locus_tag="K0W68_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HBI3072084.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(53821..54720)
                     /gene="hemF"
                     /locus_tag="K0W68_000411"
     CDS             complement(53821..54720)
                     /gene="hemF"
                     /locus_tag="K0W68_000411"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBI3072085.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(54724..55593)
                     /gene="amiA"
                     /locus_tag="K0W68_000412"
     CDS             complement(54724..55593)
                     /gene="amiA"
                     /locus_tag="K0W68_000412"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBI3072086.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTVAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            55807..56232
                     /locus_tag="K0W68_000413"
     CDS             55807..56232
                     /locus_tag="K0W68_000413"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBI3072087.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            56219..56668
                     /locus_tag="K0W68_000414"
     CDS             56219..56668
                     /locus_tag="K0W68_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBI3072088.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            56729..57304
                     /locus_tag="K0W68_000415"
     CDS             56729..57304
                     /locus_tag="K0W68_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBI3072089.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            57400..58299
                     /gene="yfeX"
                     /locus_tag="K0W68_000416"
     CDS             57400..58299
                     /gene="yfeX"
                     /locus_tag="K0W68_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBI3072090.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(58357..59661)
                     /gene="pbp4b"
                     /locus_tag="K0W68_000417"
     CDS             complement(58357..59661)
                     /gene="pbp4b"
                     /locus_tag="K0W68_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HBI3072091.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(59666..59839)
                     /locus_tag="K0W68_000418"
     CDS             complement(59666..59839)
                     /locus_tag="K0W68_000418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HBI3072092.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            complement(59852..61090)
                     /gene="murP"
                     /locus_tag="K0W68_000419"
                     /pseudo
     CDS             complement(59852..61090)
                     /gene="murP"
                     /locus_tag="K0W68_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(61094..61990)
                     /gene="murQ"
                     /locus_tag="K0W68_000420"
     CDS             complement(61094..61990)
                     /gene="murQ"
                     /locus_tag="K0W68_000420"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBI3072093.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            62154..63011
                     /gene="murR"
                     /locus_tag="K0W68_000421"
     CDS             62154..63011
                     /gene="murR"
                     /locus_tag="K0W68_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBI3072094.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            63140..63931
                     /gene="ucpA"
                     /locus_tag="K0W68_000422"
     CDS             63140..63931
                     /gene="ucpA"
                     /locus_tag="K0W68_000422"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBI3072095.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            64102..65118
                     /gene="cysP"
                     /locus_tag="K0W68_000423"
     CDS             64102..65118
                     /gene="cysP"
                     /locus_tag="K0W68_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBI3072096.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            65118..65951
                     /gene="cysT"
                     /locus_tag="K0W68_000424"
     CDS             65118..65951
                     /gene="cysT"
                     /locus_tag="K0W68_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBI3072097.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            65951..66826
                     /gene="cysW"
                     /locus_tag="K0W68_000425"
     CDS             65951..66826
                     /gene="cysW"
                     /locus_tag="K0W68_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBI3072098.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            66816..67913
                     /gene="cysA"
                     /locus_tag="K0W68_000426"
     CDS             66816..67913
                     /gene="cysA"
                     /locus_tag="K0W68_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBI3072099.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            68047..68958
                     /gene="cysM"
                     /locus_tag="K0W68_000427"
     CDS             68047..68958
                     /gene="cysM"
                     /locus_tag="K0W68_000427"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBI3072100.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(68961..69329)
                     /locus_tag="K0W68_000428"
     CDS             complement(68961..69329)
                     /locus_tag="K0W68_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBI3072101.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            69434..70285
                     /gene="pdxK"
                     /locus_tag="K0W68_000429"
                     /gene_synonym="thiJ"
     CDS             69434..70285
                     /gene="pdxK"
                     /locus_tag="K0W68_000429"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBI3072102.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT        16702 a        18350 c        19432 g        15817 t
ORIGIN      
        1 aatggcgaat ggcaatggcg aacggaatca caaacaaatt ggcgatacag tgctcaaacc
       61 cactggcgac aaacagggtg atgggcaaaa tcatggcgac gattttgtcg cataatgaac
      121 gcccgcaata actcatccac agcgccagac aaaccatcag attgcacata atgccgaggc
      181 tgacagattc agtaaatgta tgatgcattt tgccctcggc gcagtgtaaa accgcgactc
      241 cccacatcgc gttttcactc atcaccagcc cggaaaacca gattaacaaa ctgaaacagg
      301 caatacctgc cagattaccg caggccacca gaagtgcgtt aatcagccaa gttcgccaac
      361 taataacgcc ccgacttttt gccatcaccg tcattaccga cgaggtgaac aggctggtgc
      421 cacaaaccgc cagcaaaata aagccgagtg taaagcaaag gccgcccacc agatgggtta
      481 atgcctgtga agacggggca tcggcgataa cggaaaggta aaaagtaaat ccaatcgcca
      541 taaacacgcc agcggacatc gccattaaaa agaccgacac tttgctgcgg gcaattttat
      601 gggcagtaat ccgttcagcg attgccgctt ttctggcgct caactgcaag tcgaaagaga
      661 gtttgtttct catgcatact cctgcaaaag cagattacaa cacctcgcga accgagagcc
      721 ccagacgctg cattcgtgac agcagcgtgg tgcgcttcat ccctaatcgt gtcgccgcgc
      781 cacggggccc ggcaacaatg ccattggttt ctcgcaatac ctgaacaatg cgctgacgtt
      841 cttcttcgtc attctctggc gtcgtcgggt gcagcaactg cgccattgaa ctgcgaagcg
      901 ctgaatcttc atttagcgtc ggcagtaaat ggctttgtcg gacattaaga tgtaaattca
      961 gactgttacc gcgagtcagc agtaccgctc gctcaatcac gttttccagc tcgcgcacgt
     1021 tgcccggcca gtcccacgac atcaactggc gtagcgcctc ggtcgggatg gcgtcaattg
     1081 aacgattcat atggcgcgcc attttttgcg tgaaatgctt tgctaaaaga gggatatctt
     1141 ccggacggtc gcgtagcggc ggcaattcca gtgggaagac attcaggcga taaaagagat
     1201 cgctgcgaaa ctggcgatct tcaaccattt gccacaaatc acggttggtg gcggcaatga
     1261 ctctgacatt caccgggatc gttctactcc cgccgagacg ctcaatctcc cgctcctgca
     1321 atacgcgcag cagtttaggc tgaagttcta acggcagatc gccaatttca tcaagaaaca
     1381 acgtgccgcc atcggcaatt tcaaaacgac cacgatgggt attaatcgca ccagtaaacg
     1441 cccctttgtc atgaccgaat aactcacttt ccagaagact ggcggggatg gcagcgcagt
     1501 tgattttgac cagcggcttg tcgcgtcgcg ggctaagttg atggatcgct ctggcgatca
     1561 cctctttgcc ggttccggtt tcaccacaaa tcaacactgt actgtcgctc ttcgccacaa
     1621 tatctacctg ctggagcagg tcttccattg cctggctttg atagataatg tcaccgacgc
     1681 ccagattcga aaggagctgc tcgctaagct ggtggttttc ttgctgcaaa ctttcctgca
     1741 aatcggtcat gctacgccag gcatcggcat tgccaacggc aatagcgatg cgatcggcta
     1801 tgtgttgtag tagctgacag ttttcctcac taaagagagt ggaagaggta tgcgccagca
     1861 acaatgcacc cggtgtatgg ttgccaaagg taagcggtat aaggagcgca gattcgcagc
     1921 cgttagatgc aagtaagagt aataacgggt cgcgttgcca gagaaacaga tcgtctgcac
     1981 ggtgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt cagcaataca ctgtcgccag
     2041 gcatattgcg tggcagacac gcacaatgtg aggcagaaag atcgctgcac cacaggctaa
     2101 acttctcgtt ctttcgatga tcgcccagta ccatactgac tgaagccaga ccgaaaaaat
     2161 gatggatctc acgagcgacg tcagcgatca gatcgtcgag atcaagatgt gacagcactg
     2221 cattagtaat atcaaccagt acccgatgat gatcgcgatc tttctccagc aaacgcagcg
     2281 ccacctgctg ttcttcatca ttgagccatg attgcagaac cggggtgatt atccgcgcca
     2341 gccagttaaa cagtgtgcaa tcctggggag taaaacggca gccgtgggtg cgaataaagc
     2401 gtagctccgc cattccacta ctgccgagaa ggacggcgtg acgcgaataa tcgaccatct
     2461 cgccctgata ctctgttgtt gccgtcacat cgctgccatc atcatggtag cggtaataga
     2521 agttgctttt gagaacatgc agttcaataa gttgtaccgg gctgaacggc ggcggcagcg
     2581 ggatgaaggc gcgtaataaa gacgccttac cgtgtttctg cgctaaccgc tccgcgagca
     2641 ttccgtttac cgcttcaatt gttattcctt gcggcggggc aaacatcgcc tcgtctgaca
     2701 tagccatcaa cagtccttat taatgagaaa tccttctcac gcaatttttc tagccatcag
     2761 atagatggcc ggttcctgca caatttcatc caacagatga atgaattgaa tgctacgcgc
     2821 ctgccacaca gacgtcgcgt catccgccag tggcgcgaat gcccgggtca acagggcggt
     2881 gtggctggcg tcaataacaa tctcaccaaa agtcatcacc cccagcatct tacgtcgggc
     2941 ttgctcctct tcgaccagtg caagccaatc ttcgtattcc gtcagtgggc aggggaaggc
     3001 gacattcaga caatcaatca cgccaacgtg atgaccgata gccagcgagt aatacattac
     3061 ctgagagctg tgttccggca tctcgtcgct actggcgaca aacttttttc gcagcgtcca
     3121 gaaaacaatt tccccgcact cttcagtcat gtaacccccg gagtacgcga aagagttgct
     3181 gaacgatact attgagacgt ggatcgtcgg catcgcgcaa ccaggtgtta acccgatttc
     3241 ctgtaggatc gtcgcttaaa tggcgcagga gccgatcgca aatttctcgc ccctgacgat
     3301 agcccgccag ccgccgcgct tctcgctcaa tggcgatacg ctgtgatggc gggatctgcg
     3361 gccacaacgg ttgcatagtc accccggtgg gatcgcgata atccaccgcg tgaatcttct
     3421 gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt ggcggccggt gttggcgggc
     3481 agccggggat ccaaacatca atggggacaa tggtgtcgct gccgccccag acgctgtaga
     3541 gatcgtggaa aataccgccg ccgacaccgc acgcgccgta ggaaacacaa attttatgat
     3601 cgggggcaga ctcatacgcc cgaagtgcag gcatacgcat cgcccgggtg actgcgcctg
     3661 taaataacaa aatatcggcg tgacgcggtg atgaaataac cttaatgcca aaacgttctg
     3721 cgtcgaatac tggtgtgatg gcggcaaaaa tttcgatttc acaggcgttg cagccgccgc
     3781 aatcgacgcg ataaacgtaa gccgagcgac ggatatcctg tagcaaatgc tgcttcatct
     3841 tttgcgtttg ctcgtccagc gtgatgggct ggctgacatg ttgtgtaagc actggactca
     3901 tagctgctcc ttagccacca gcggtacatc tgtatcgtcg ttgagcagcg tcgcgcgttg
     3961 tttgcactcc gggcagacgc tcgcttgcgc ccataacatt tcgcggtttt gtggcgcatt
     4021 ttgttgctgt gccaacaatt cagcagccag tgcgacggtt ttttgcgggg caaacgggcg
     4081 ttcgcaacgg ctgcaacgtt gtagatggaa cgtcgcgcgg gtatagagat cggctttatt
     4141 ggtgacggtc agttcaaagt tattggtaag ctggatggct ctggtcgggc acacttcttc
     4201 acaacgtccg cagtaaatac aacgcctcag atagagctgc caggtgcgcg aattttgctg
     4261 gtcgtcggtc tggatagtca gcgcatttgc cggacaagca caggcgccgc aggcaataca
     4321 ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa
     4381 gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatctt
     4441 ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata
     4501 cggcacggtt tttgactggc gcttgcgcac atctaccagc gtcacacggt cggtacagga
     4561 gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgcag
     4621 catgtaacgc aacaccggcc agttggcgta ggtggcggca cggcagcgcc agcggaacaa
     4681 tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa
     4741 gcccagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg gggtatccgg
     4801 catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt tgacacggac
     4861 catcacgcgg gagaaaacat cgccgccggt aaaggtaaac agtgtttttg gaatattgcc
     4921 gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc cgcggatcag
     4981 cggccctaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt
     5041 gcgttgttcc atattcggcg tggcaagcag catctctacc agctccgaca cgtcggcgcg
     5101 catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat cgcggcgaac
     5161 accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt ccgccattgt
     5221 catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaatc caacaaaatg
     5281 gcaggagagg ccaaggttga gcaaatgact gtgcagacgt tcgacttcca gcagaatcga
     5341 gcgaatagta tgcgctcgtt gcggcacctc aatccccagt gcattttcaa ccgagttggt
     5401 ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cgatccgata agaaggtcac
     5461 ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga cataaaacag
     5521 gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt gacccggttc
     5581 atcggaggtg atatgcagcg ggccaatagg gatcacccgc gcatcgctat tgccctcatt
     5641 gataaacgga tacgtttcgg tatcagtcgt cggttcaggg cgcagtcgat aatccatcgc
     5701 atctttgcgc agcggatgca tatcttccgg ccagttatcg ggcaacacca ggcgacgctg
     5761 atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct cgccccagac
     5821 cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat cgaccagtgc
     5881 cttcaccacg atccagcatt tttcggcccc ttccattgaa agggcataat aaaccgcgta
     5941 atgaccgtta agtgtccgct cgtcgttgcc aaacaggact ggaagccagc catcatgttg
     6001 ataataaaga taatgtacaa cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg
     6061 ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt tcagggcggc
     6121 gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc tccgaaacag
     6181 atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg
     6241 tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt tgtggaatat
     6301 gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg gtcaaccagc
     6361 cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc gctgccatcc
     6421 gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata atcagcaggt
     6481 gattacgtgc cagtccggcg gtaatggtca taaattcgct aagaaaaatg ttgaagggcg
     6541 gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat ggcatgattt
     6601 tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt acattgccgg
     6661 aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa gatgtgcagc agcgcggcaa
     6721 aaattcccag cggcccgcca atgcctagcg ccaccgcgac cagccccatg ttctccacgc
     6781 tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat gccgccacgg
     6841 caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg ccgatggctt
     6901 ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc aatacggcgg
     6961 agagcagggc gctgaccgga ctcggcgctt cactgtgagc atccggcagc caggcgtgca
     7021 tgggaaatag cccggttttg gtaccaaagc caattagcac aaacacaaag gccaacagca
     7081 ttaatgttgg gtcaagcaag gacgattgct taagaacctc gctccagaat atcgccattt
     7141 ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg aacagaccaa
     7201 aagcgacacc aacagtacaa ataatgatgt acttccatgc tgattccagc gatgaacgct
     7261 gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc gccgcccaca
     7321 tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac agatggaaga
     7381 aaccgtagta atcgcacagc gtaacgggtg aaagctcgcc gtgtgccact tcatgacgca
     7441 tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg gcgaggaaca
     7501 aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct cctgcatcag
     7561 cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct aagctatgta
     7621 atacagtcac cgtacaagtc gcagagagcc cccgtttccg gcaggcaaaa cagagcagcg
     7681 aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc atctcattat
     7741 cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca tatttttctt
     7801 gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc tatttccacc
     7861 agttccggtg ctcgccaggc aagaagcgcc agcaccagat gggagccgtt ttccatcagg
     7921 cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag caggccaagc
     7981 agaaaatgac ctaacgctac cgccagcgcc ggtttcagcc cggtagccat cggtagcttc
     8041 acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag tgccatcatt
     8101 gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc agcgtaagtc
     8161 atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca gatcagcagt
     8221 tgctctgcgg cgaacaggca cgagagagtg gcgaaaatag acaccagcac cagcgactgg
     8281 caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa cagcgatgtc
     8341 agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca tcatctcctt
     8401 actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc agtacaatca
     8461 acaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg ctcggtttgc
     8521 ccggcacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc gattcaatca
     8581 tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca aaaccgacgg
     8641 caaacagcgg gaatttactg aagaagccgt tgaacggcgg cacaccggta atcgccagcg
     8701 ctgccacgca gaaacccacg cttggcaacg gcagggtgtg caatacgcca cgcagacgcg
     8761 gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa aacaggcttt
     8821 tagcgaacgc gtggttgacg atgtaggcga tgctaccctc cagcgccagc cgcgagccga
     8881 agatggagag cgacaagccg aagaacatcc agccaagttg agtgatggtc gaccaggcca
     8941 gcaaccgctt catatcctgc tgtggcaaat acatcagaaa gccataaaga atggtgacca
     9001 gcgccatgac catgccaacg ccgccaatca catgcgggat attgccgccg tcgataatgg
     9061 cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga taggcactga
     9121 tcggtgttgg cgcttccatt gcatccggta gccacgcttg catcggtagc tgggccgatt
     9181 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccgtgtaact
     9241 cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
     9301 acaaccccaa cgagccgata tgagtgataa gtaacgcttt tagtgctgaa agctgtgctt
     9361 tatcgctctg gtaataactg atcaacgccc aggagcagcc gcccgtaatt tcaaaaaaca
     9421 acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg ataaacacca
     9481 gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt ttatccgtca
     9541 gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca aacagaatca
     9601 gcgtactcat gcgatcaatg accaagccaa acaatgccac atcccccacg ttgaccaacg
     9661 tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagcgac aacatgcaca
     9721 gcgtggtcag tgcggcgaac aaaaccccca ttcggcggcc cgacgttgtg gcgaaaacga
     9781 aacgaccagt gcgccgataa aaggcagcat taacgtcgtc agagcaagat tttccatatt
     9841 ccgtcagtgc tccttacaga ccggttaacc agaagaccca tgcaagcgca gcaaggctga
     9901 agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg
     9961 cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca
    10021 gcaggcaggc gagagaaagt tcttgcgcgc gcccaaaggg cagaaacagg gccacaaaca
    10081 gtgctgccat cacgacctgt ttcagcccca gcccccattt cgctagcgcc agcccggcac
    10141 cggaatattc ggtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg
    10201 gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg
    10261 ttagcggcga gttccagccc gttgccagcg tattgctgat catctcgata tgcgtggaac
    10321 ctgctatcag cgccaatacc agcagtgaga gaatgagcat gggttcgacc agaatgccga
    10381 gcgtcaactc gcgactggca ccgactcccg caaacgggct tccggtatcc agccctgaaa
    10441 gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga tcgccgccgc
    10501 ccgcaaaagg ggaaacggta ataaatagtg gtagggccat cgccagcacc agcatgctgc
    10561 tgattaatac ccacggcatc aggcggaaca tcagacctga agatgtcggc gcatcttcct
    10621 ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg
    10681 agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagtggggtt agcatcagca
    10741 gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca
    10801 tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac
    10861 aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttggc cagccgttgg
    10921 gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc
    10981 ctggcggtga cgtgcgcaag acctttattt agcctcagcg tagggtcgag ctgttgtcgt
    11041 agacgaaata gcgcagaaaa gaccacacgc agcggctgca tcacgccatt gcctgacggg
    11101 gccatcgcat tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct
    11161 gcgcggcgcg aacgacacca cagccagtaa aggccaggca gcaaaggcat cgccagtagt
    11221 aacagcaaca gtaatgaagg agtgacccgt gtatgaaacg tgccgggtac aagtgctatt
    11281 ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatgtg cattatcttc
    11341 ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg
    11401 gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtgct gcgcggcgca
    11461 ccacagaaag taataccgta cattttgacg aagcacatta ctgccagccc accagtgact
    11521 gccagcatta ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt
    11581 agtgagaata gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaatg
    11641 gctgatatcg ccaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc
    11701 agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc acccgcgccg
    11761 agaaatagca gccctttgaa aagcgcatgg ttgagcagat gaaacagtgc gccgagcagt
    11821 ccaaccacgg tgagcagcgg gtcgtgcagt gacagaccga ccatcgccac accgaccgcc
    11881 agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt
    11941 tccgccagtg catacagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga
    12001 atgccccacc acagcggcaa acccgtttgc gccagcagat ccatcgccac tttcaggatg
    12061 ccgaaaatac cgattttgac cattacgcca gacatcaacg ctgaagcgtg cgatggtgcg
    12121 gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa
    12181 ccgaaaaagg ccagcaggaa caccgccgac gccagccccg gagaaagtga aagcgtgcgg
    12241 aaactggcaa aatcgaggct gccgctttcg cgccccatca gcaagaaggc gatcattatc
    12301 agcaccgatc cggcgtgggc gataaaaaag tagagcatgc cagcgttgat cgacgtttta
    12361 tcctgcctgg caatgaccag aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg
    12421 aaccaaaaag cgttgtccat caccagcagg gcaaccatcg atgcgatgaa aatattcata
    12481 aagaagccca tcgccgccgc gcctttgccc tcgtattcgc gcatataagt caatgaatag
    12541 agagaacaaa ccaccaccag caaggagata accagcacca taaatgccga gaggctatcc
    12601 attcgcaggg ttaaatcggc aaattcaaaa ggcgtggcaa aacgggcgac taaagttacg
    12661 cctgcatgta attgcgtaat tccgctgatg atgccaatca cgccgcccac cagcgatgtg
    12721 atgccggaaa gcttaatagc cagtctgtcc agaccgagta aaaacagcga agccagactg
    12781 gcaaacagat agaggatcag cgaccaggtt aataattgca gggcatccat tagcgttgct
    12841 cctgagtgag ggaaagggga tcctccccgc cattggcaaa ccagcttgcg gcaaggcgct
    12901 gtttttcttt catctggcgt tgcaggctat cgtcggtgat cagccgtaac gcctgattcg
    12961 ggcaagcgcg aacgcaggcc ggaccttctg gcaagaaatc acacaggtcg catttcaccg
    13021 cgacggtctg gacacccggt tcccagcgca gcaacgcatg ttggggcggc acgttttctt
    13081 cgccgtcttt gagtgagccc tcagcctgaa aaacatattg cgcaggggca ttcaccggac
    13141 ggcttcctga agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa
    13201 tacagaggct ttcgttgagt tggatcgcat catccctctg agagatggca ttgaccgggc
    13261 agacctgcag gcaaggggct tcctcacagt gatgacacac gacaggggca gtgagtgttg
    13321 acgtcttcgc cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg
    13381 aacaggcagc gagacaggta ttacatcctg tacaccacag tggttcggcc accacaaagc
    13441 ggttcatgtc ggctccttga aaatgatatg aaacattgca tttcatattg cataaaggat
    13501 gctattctga ttaactcaat atattcaata agttatgtat taactggatt ctgaacggta
    13561 caaaaatgat ggtcattaac gttcagcaaa aaatggattc gacacccgtg tcgcagggga
    13621 aagcgagaat gtgatcttga tcacgagaag tgaggtatgc tggccgcaag cgcagccagc
    13681 acggaatgga gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt
    13741 gattgctggt tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga
    13801 agctgatgcg atgaatgcca tcgtaggttt tgcccatgaa ggacttttca ccccagacgc
    13861 cgaattgttc gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt
    13921 tttccgcaaa acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc
    13981 ccgctttttt caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg
    14041 gggtcatggc tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat
    14101 taacttgttc tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca
    14161 gtggattcat tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat
    14221 actgccttgt gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc
    14281 gtccgcagat gcggggctgt gggcggttat ctgaatatgc aactgcgcag cccgttcatt
    14341 ttcagcaggt tgcgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag
    14401 tgctgtgaag cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga
    14461 tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag
    14521 ctcggcacct ttcaacggta acgttgattc aatcagagta atggcattcc atgaaccgga
    14581 aagcagcatg ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt
    14641 acagccgcaa ctactgacat gacgggtgat ggtgttcaca attccagggc gatcggcacc
    14701 caatgcagtg atcaccagat aatgttgcga tgacagtgtc aaactggtta ttcctttaag
    14761 gggtgagttg ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc
    14821 tgtctgcttg cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg
    14881 atggcccatg ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa
    14941 tgtctgtcgg gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc
    15001 gatcgtttct gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga
    15061 tgtggtgatg atgacgctgg agctggctga tgggcgcatt ccggtaattg ccgggaccgg
    15121 cgctaacgct actgcggaag ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt
    15181 cggctgcctg acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca
    15241 tttcaaagcc atcgctgagc atactgacct gccgcaaatt ctgtataatg tgccgtcccg
    15301 tactggctgc gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat
    15361 cggaatcaaa gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc
    15421 agatgatttt gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattagg
    15481 cggtcatggg gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg
    15541 caaactggca gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc
    15601 attacacaac aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga
    15661 actgggtctt gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg
    15721 tcgtgagacg gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt
    15781 tgatggctta ctctgttcaa aagtcgcgcc tggcaaaggt tgcgggtgtt tcgcttgttt
    15841 tattactcgc tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag
    15901 cctacctgga agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg
    15961 tgacctccgg tgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc
    16021 tggacattcg tccaccagcc cagccgctgg cactggtttc tggcgcgcgt acccagttca
    16081 cgggcgatac cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg
    16141 tggttagcgt gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga
    16201 cactgaccac cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc
    16261 gttatcaaat ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgttga
    16321 acctggaaca ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt tacagcacgg
    16381 agatgatgaa cgttatttcc gccggtctgg ataaatctgc cactgacgcc gcgaacgctg
    16441 cgcaaaatcg tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac
    16501 caatgctggt cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gcggcgctgg
    16561 aaaaagtggg catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt
    16621 ataagccgct gtctgacagc gactggcagg aactgggcgc gagcgatcca ggcctggcat
    16681 ccggtgacta taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg
    16741 atccgaaagg tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg
    16801 ctgcgtttag caagtaaaaa tacagggctg gaatcatccg gccctttttt ctgatatgat
    16861 acgcaaacgt gtgcgtcggc aggaaaacgc gattttagcg gtaattcgca cgaaatttgt
    16921 ttgtcggacg tagttcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc
    16981 agacagcaaa agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca
    17041 agctgagttg tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt
    17101 ggtgctcgaa ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga
    17161 tcgcaaaggt atgttgaaca acaagttcaa ctacttcatt atgagcaaac tggctgaagc
    17221 gggtatcccg actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct
    17281 ggatatggtg ccggttgagt gtgtcgtgcg taaccgtgct gcgggctctc tggtgaaacg
    17341 tcttggaatc gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa
    17401 cgatgccatg cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtgag
    17461 caaagagaac ctggcgcgta tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa
    17521 actgttcgat gatgctggtc tgattctggt cgacttcaag ctggagtttg gtctgtacaa
    17581 aggtgaagtg gtactgggcg atgagttctc cccggacggc agccgcctgt gggacaaaga
    17641 aacactggag aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaagc
    17701 ctatgaagcc gtcgcccgcc gcctgggtgt acagctggac tgatttttct gttcatcatc
    17761 ttgccgtgca gctggcacgg caagacaacc gctctcggaa tatatgcatt gaacgctcat
    17821 tcctccgcca tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac ctacgatcat
    17881 ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac
    17941 aatgtcgaag acaggcgtaa cagctccggc ggtccgtcaa tgggcggtcc cggttttcgc
    18001 ctgccaagcg gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc aggctactat
    18061 ggtgttgatt taaccgggtt gatgaccgga cagccggttt cccaacagca atcaacacgg
    18121 tcaattagcc caaatgaaga cgaagccgca aaattcacct cggtgattct ggcaaccacg
    18181 gaagacacct ggggacagca attcgagaag atgggtaaaa cctatcagca accgaagctg
    18241 gtcatgtatc gtggaatgac gcgtaccggc tgcggggcgg gccagtccat tatgggaccg
    18301 ttctattgcc cggcggatgg cacggtttat atcgatctct ccttctatga tgacatgaaa
    18361 gacaaactcg gcgcggatgg cgattttgcc caggggtacg ttatcgccca tgaagtcggt
    18421 catcatgtgc agaaattgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg
    18481 acgcaggcgg aagtaaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc
    18541 ggtgtctggg ggcataacat gcagcagcaa ggcgatctgg aagaggcgct gaacgcggcg
    18601 caggccatcg gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagacagt
    18661 ttcactcatg gtacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc
    18721 gatccggcac aatgcaatac ttttggtaaa agcatttaac ttccggggca aggatggctg
    18781 aactgactgc gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc
    18841 tggtgttgag cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct
    18901 tacctggcga ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttctccct
    18961 cggcgctaca aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg
    19021 gctttgatgc cgctgccttt gcggcactta gcggaacgtt gaaagcggga agctggctgg
    19081 ttttgttact ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct
    19141 ggagtgattg ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac
    19201 ttacggcgga taacgacgct attctctggc ggcaaaacca gccgttctcg ttggcgcatt
    19261 ttaccccccg tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac
    19321 tcttacagca gctactgacc atgccgccgg gcgtggcagc ggtaacggct gcgcgtgggc
    19381 gcggtaagtc cgcgctggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca
    19441 ccgcgcccgc aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct
    19501 ttattgcgcc ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgatg
    19561 aagccgcagc catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt
    19621 tgttaaccac tacggtgcag ggctacgaag gtaccggacg tggttttttg ctgaaatttt
    19681 gcgctcgctt tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg
    19741 gatgcccgct ggaaaaaatg gttagtgagg cactggtttt taacgatgaa aacttcaccc
    19801 atacaccaca aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgatc
    19861 cagaaacgcc gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acctcgccgc
    19921 tggatttacg ccggatgatg gatgcaccag ggcaacattt tttacaggcg gctggcgaaa
    19981 acgagattgc cggggcgttg tggctggtgg atgagggcgg attatctcaa gaactcagtc
    20041 aggcggtatg ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg
    20101 cgcacggcag caatccactg gcggcgacat tgcgcggacg gcgggtcagc cggattgcag
    20161 tccatccggc gcgtcagcgc gaaggcgttg ggcaacagct cattgccagc gctttgcaat
    20221 ataggcctgg cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt
    20281 tctggcaacg ctgcggtttt gtgctggtgc gaatgggtaa tcatcgtgaa gccagcagcg
    20341 gttgctatac ggcgatggca ctgttaccga tgagtgatgc gggtaaacag ctggctgaac
    20401 gtgagcatta ccgtttacgt cgcgatgcgc aagctctcgc gcagtggaat ggcgaaacgc
    20461 ttcctgttga tccactaaac aatgccgtcc tttctgacga cgactggctt gaactggccg
    20521 gttttgcttt cgctcatcgt ccgctattaa cgtcgttagg ttgcttattg cgtctgctac
    20581 aaaccagtga actggcatta ccggcgctgc gtgggcgttt acagaaaaac accagtgacg
    20641 cgcagttatg taccacactt aaactttcag gccgcaagat gttactggtt cgccagcggg
    20701 aagaggccgc gcaggcgctg ttcgcactta atgaggttcg cactgagcgt ctgcgcgatc
    20761 gcataacgca atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga
    20821 gtcagcggcg taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt
    20881 gttgttcaaa gcccggataa acctgcgcaa cagttgttac tgcttttcca cggtgtcggg
    20941 gataacccgg tggcaatggg ggaaataggc agctggtttg cgccgctatt tcctgatgcg
    21001 ctggtggtga gtgtcggagg cgcggagccg agcggtaatc cggcggggcg tcagtggttt
    21061 tcggtgcagg gtattacgga agataatcgc caggcgcgtg tggatgccat catgccgacg
    21121 tttattgaga tggtgcgcta ctggcagaaa cagagcggag taggggctaa cgccacagcg
    21181 ctaatcggtt tctctcaagg cgcgattatg gcgctggaga gcattaaagc cgaaccgggc
    21241 cttgcttcac gcgtcattgc ttttaacgga cgctatgcga gcctgccgga aactgcgtct
    21301 acagccacga caattcacct gattcatggc ggtgaagatc cggttatcga tctggcgcat
    21361 gcggttgcgg cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg
    21421 gaggatttag gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt
    21481 tataccattc cgaaacatta tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat
    21541 gatgtgattg aaatgatgta aaagacagat tacctggcct gataagacgc gtgagcgtcg
    21601 catcaggcat cgttcaccat tgccggatgc ggcgttaacg ccttatccgg cctacggttg
    21661 agcactcagc ttacttcttc ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact
    21721 tatcgttgaa atcacgatgc gggggaagct ccggcttgtt ggcgagaaat ttcttgtggt
    21781 cgacgcgctt gagatcttta atcacgttca gcaggacgcc taccaaaaag acaatcacca
    21841 gaatccacca atatttagcc agccagtcca tgcttatttc ctcttgcaga accactcatc
    21901 aggcgacgag ctgttccatg atacgttgat acatacgggc aagtagctgc aggtcggcag
    21961 cgttcacaca ttcattaatt ttatgaatag tggcattgac cggcccgagt tccaccacct
    22021 gcgcccccat gcgagcaata aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg
    22081 gtttaatttc attatagtgc tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg
    22141 cggtcaaaaa tggctgcccg gaaagccacc aatccaccgt atagcgcagt tgatgttttt
    22201 caagcagggc aagcacctgc gctttgatca tctcatcagt cagttcggtg ctgaagcgga
    22261 agttaaactg cacaaacagt tcacccggaa taacgttgtt actgcccgtt cccgcctgaa
    22321 tattggcaat ctgcatactg gtcgccggga agaattcatt gccctgatcc cactcaatag
    22381 ccactaattc attaaggaaa ggtgctgcgc gatgtactgg attgtcagcc agatgtgggt
    22441 aggccacatg cccctgcacg ccatgaatgg taaggttgca ggttaacgat ccgcgacgac
    22501 catttttcac cacatcacca accacttcga tactcgacgg ttcgccgacc agacagtaat
    22561 cgagacgctc attacgcgcc attaacgctt cgacgacttt taccgtaccg ttgtgggcac
    22621 tggcttcttc atcagaggtg atcagaaatg ccagtcgtcc cgtatggttg ggatgttgtg
    22681 cgacaaaacg ttctgctgct accaccatcg ccgccagcga gcctttcata tctgccgcac
    22741 cgcgcccgaa taacatgcca tcacgaatgg tgggttcaaa cggcggattg atccaacgat
    22801 cggcgtcgcc aggcggcacc acgtcggtat gcccggcaaa ggccaatgtt tcaccttgcc
    22861 cacgccatgc ccaaaaattc tgcgtatcgg caaagtccat gcgttcaacg gtaaaaccga
    22921 tcgcctgcaa acgatcaatc aacaaagcct ggcatcctgc gtcatcagga ctcagggaag
    22981 ggcggcgaat aagctgttgt gtcagctcaa taaccgggca cgacatagac tacacctcat
    23041 ggaaaaattg ctgataactg gaatcactga aacccagcag cataggctta ccgggcgcgc
    23101 agagcaatgg acgtttgata attgcaggca tttcagtcat taatgccgcc gctgaggccg
    23161 catcggtgat tttattgcgg gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt
    23221 tgagtaacgc ctcccaacct aattcgttga taaaaccgtt caataattcg ctgtccagcc
    23281 catcgacgcg gtaatcatga aaacgatagt cgatgttatt ggcttccagc caacggcgag
    23341 cctttttaat ggtgtcacaa tttttgatac cgtaaagtgt aaccataatg aatccttttt
    23401 cgatttatga gaataaatac tcatttaagg gtgaaaacat tttgcctgca aatgattatc
    23461 aggaaatata acattatcga agtattcaaa ttaacaacta ttaaataata ttaaacattt
    23521 ttaacgtaaa cattaagaat attcatcttg ttatctgtct gtattttaac ctgaattaaa
    23581 tcagtattag atgccgtatg aatatctcga aatgttgctg tgtatcacat acaaatcatc
    23641 accgcaggac catagtattt acataagatt tgtccccccg gaggtagtca tcgcagcaat
    23701 cgagtaaatt agcttcacca cggtatattg tttccgtaga atacccataa ttataagaga
    23761 ggttgttatg attgaacgtg aactggggaa ctggaaagac tttatcgaag ttatgcttcg
    23821 taagtaattc ctgagacaaa gtataaacag caagaacccg ctgagcaggt tcttaatcgg
    23881 aaagttgaaa aaggcgacac attggcatgt cgcctttttt attgcttatt ccgggcgcgg
    23941 ctttagcggg aagcggcggc gcaccagcac aaagaacagc ggcacgaagt aaatagccag
    24001 aatagtggct gaaatcatcc cgcccattac gccagtaccc accgcatgct gaccaccgga
    24061 accggcaccc gtgctggttg ccattggcaa tacgccgaag ataaatgcca gcgaggtcat
    24121 cagaatcggg cgtaaacgct gacggcaggc gtggagcgtc gcttcaaaca ggtcgtggcc
    24181 tttttggttc atctcgttag caaactcgac gatcaggatg gcgtttttcg ccgataaacc
    24241 aatgaccgtt aacaggccca cctggaagta aacgtcgttt tccagcccgc gcatccaggt
    24301 tgccagcagc gcgccgatta cccccagcgg cacgaccagc attaccgaga acggcaccga
    24361 ccagctttca tacaacgcag ccagacacag gaataccacc agcaaggaaa tggcgtacag
    24421 cgccggagcc tgcgcgccgg aaagtcgctc ctgatacgac atcgccgtcc actccagacc
    24481 aaagccgttt ggcagctgct tcactaacga ttccataata tccatcgccg taccggtact
    24541 gacccccggt gcggcttccc caacaatctc aaccgcagaa taaccgttat agcgttccag
    24601 acgcggcgag cctgtttccc agcgtgaggt cgcgaaagca gagaagggca ccatgccgcc
    24661 atctttattt cggacatacc agagattgat gtcatctggc agcatgcgat acggcgcagc
    24721 tgcctgcaca tagactttct tcacgcgacc gcgatccata aagtcattca catagctcga
    24781 accccaggcg gtttgcagtg tgtcgttaat atcgtcgata gcaacgccca gcgcctgagc
    24841 tttacgctgg tcgatatcaa tctgcaactg cggactgtcg tcgaggccgt tatggcgcac
    24901 acgggttagc tccgggtttt ccgccgccag cgccagcaac tggttacgtg ctgccatcag
    24961 cgcatcgtga cctgctccag cgtggtcctg caactccata tcaaaacccg cagaactacc
    25021 aagaccgcta attgctggcg ggctgctggc gataacgcga gcttctttaa tcttgttaaa
    25081 cgcctttgtt gcacgctcga taatggcaaa cgaggtgccg gtcttactgt cgcgttcgct
    25141 ccagtctttc aggcggataa acattcgcgc cacgttttgc ccgttacccc cagggccaga
    25201 accaacggtg gcaaacaccg acatgatgtt gtctttttca tgggtgaagt aatatttctc
    25261 gatttgctca acgactttca gggtctgttg ttgggttgaa ccgctgggca attgtaccga
    25321 ggtagtaaac atgccacggt cttccagcgg taagaacgac gtcggcaaac gcaggaacag
    25381 gaacaccatg ccgccaagca gcaggacata aatcacaatc cagcgcaggc tgcggtggag
    25441 aattttcgcc accccttttt cgtagcgttc ggcgttgcgg ttaaacatct ggttaaacca
    25501 ggcaaaaaag cctttttgcc cgtgatgctc accttttttc agtggcttaa gtagtgtggc
    25561 acacagagcc ggagtgagga tcatcgctac cagtactgac agcaccatcg ccgcaacaat
    25621 ggtaatagag aactggcgat agatggcacc ggtggtgccg ccgaagaagg ccattggtac
    25681 aaataccgcc gaaagcacca tcgcaatccc gaccagtgcc ccctggatct gccccatcga
    25741 tttacgtgtg gcttcgcgag gagtgagtcc ttcctcactc ataatacgtt cgacgttttc
    25801 caccaccacg attgcgtcat ccaccagcag accgatcgcc agtaccatcg cgaacatggt
    25861 taaggtgttg acgctgtaac cgaaggcgta aagtacggag aaggttccca tcaacaccac
    25921 cggcacggcg atagtgggta tcagcgtggc gcggaagttt tgcaggaaca gatacataac
    25981 gaggaaaacc agggcgatag cttccagcag cgttttcacc acgtcttcaa tggaggcttt
    26041 aacaaacgag gtggtttcat acgccacctt gtattccagt ccgtgcggga agtactgcgc
    26101 cagctcgtcg agacgattga gcaccagctc cgctgtcgcc atttcgtttg cgccggaggc
    26161 cagttttacc cccagcccgg aggctggctt accattgaag cggctaagat aatcgtattt
    26221 ctccgccccc atttcgacgg tggcgacatc gcccagcctt acctctgagc cgtcttgatt
    26281 cacccgcaag gtgatatcgc ggaactgttc tggtgtttgc agcagtgact gggcgttaat
    26341 ggtggcgttg agcgcctgct tatcgacgga aggtgtacca ccaagttgcc caaccgcaat
    26401 ctgcgcgttc tgtgactcaa tggcatcagt gacatcttta gccgtcatct ggaaactgtt
    26461 gagtttcgcc gggtccagcc agatacgcat ggaatattgc gaaccatagg catcgatatc
    26521 cccgacgcca ttcacgcggc ttaacgggtc ctgaatatta ctggcaacat aatcggcaat
    26581 atcctgttta tccatcgaac catcggtaga gacgaaagca atggtcagaa tattggtatc
    26641 gccggtttta cgcaccgtca cgccctgatt ttgcaccgcc tgcggtaact ttcgcatggc
    26701 tgattgcagc tggttttgta cttgctgcac ggcttcgtcc ggatcggtgc ctgctttaaa
    26761 acttaaagtg acagatgcct gaccgatgcc actgctctga gatgacatat acatgagatt
    26821 atcgaggccg gtcatatttt gctcgataac ctgggtcacg gtgttttcca gcgtctgggc
    26881 cgatgcgccg ggatagttag cggtcactcg cacattcggt ggcgcgagat cggggtattg
    26941 ttcaacgggc aatgaaaaaa tcgccagggt ccctgtcaga cacaacagga ttgccagcac
    27001 ccaggcaaaa atggggcgat caataaagaa attcgccatt aaaagaggac ctcgtgtttc
    27061 tgcgtatcgt tgtaggcttg acttgcttca ctgtagcggc aaggtacgag ccaatcgtgg
    27121 agaaaaaaag gagttaatgt aaattatcat gcccttttat actgaacctt aagtgcaagt
    27181 attctttggt cagtaggagg cattacatca actgactttc ctcaccctcc gcagagcgaa
    27241 agctaatact cactaacgtg ccgccgccgg aaggtggtga aaaagtcagc gtcccaccca
    27301 gccgttccgc gcgttcgcgc atgatattca gaccataatg accttcgggt tctttcggtt
    27361 caccgatacc aatcccgtta tcacggatat aaaccgtgtg attgccgtcc ggcgcggtga
    27421 cgcaactgac ggcgatttcg ctggcgttgg cgtgcttcat cgcattcagc accgcttcgc
    27481 gaataatttg caacaaatgc acctgcattt gcgcatccag cgccagggtt ggcagacggc
    27541 agtcgagggt cagtttggcg ctggtttgat tttgtaacgt atccagcatt tccctcaacg
    27601 cggaggggag atccgcctgc tgcagcgtca ggcggaaagt agtcaacagc tcgcgtaact
    27661 gccgataagc atcattcaac gcctgggaaa aatcggccat gatactttgt gcggtggcgt
    27721 tatcttccgg tatcgaacgc ttcagtaacg tcaactggat acgtaagtaa gaaagtacct
    27781 gagccagcga gtcgtgcaat tcgcgggcga tggttgcacg ttcttccatc aacaataact
    27841 gctgaaaatg cttttgcgcc tgattaaagt acaaaccgcg tcccagcatc gacgaaacgc
    27901 tgttaagcag cggttctgat gatgaaacgt gactattttg ccagtgcagt tcgccgtaaa
    27961 ccgtctcttg cattgtcacc ggtaaaatct gcatcggcaa ttccgggttt ggttgccctt
    28021 cgctaatccg ccagttttca ccgacattta actccagata ttcagccgct tcattgtcgc
    28081 gaacaatctg caaaatatgg cggaaacaat gcacatcaat ctggcttgtg ttcagcgcct
    28141 gcgaacattg atacaacacc tccagacgac gcttggcctc gtggagatcg cgggtctttt
    28201 cttctactga cgcctccagc gaaaggtaca atttatgcag ctcgctcgac atctggttaa
    28261 aggtttttgc aagcagacca agctcattcg gcagactggt atccagcggc ggcgagtcga
    28321 actgcccgtg ttcaatacgt tgactggcgg taaccagctg attcagcggg gcaaccacct
    28381 gatggcgtat acggcgcaaa gtaaaaaaga ccagcgtgaa aataccgata ccgccagcca
    28441 gggaaatcgc caccaccagc agcattttgc gttcagcgta gtgctgtaaa gccagcacga
    28501 acaggtctat ctgattaaca taattattaa tattggcctg ataccacggc aaatcgccct
    28561 tgctgagccg attattcatc tccagccagt tggcattcag atgcgcatag cgagttttta
    28621 ctgcttctgg cacataccag acgttgagat tggttaatac cgatgaatgc agtgcctgct
    28681 gaaacaactg gcgatgtgca ttgagttgtg gactgccact ttgcaagtcg tagcccaggc
    28741 ggtaactctg catacgcagc gatccggcaa tattgatagc ctcagcgtcg cgcaaactgc
    28801 tcgccagagt tagcagagcg atacccgtgg aaagaatcga cagcagcaca atgtaaaaaa
    28861 aggcccgggc cagactggcc gagacgggtc gtttaacaat cacacgcgtc ttctccacaa
    28921 aaaatcatta atgttccagt tcgctaaaac gcaatttagt cagtgatggg attatttgat
    28981 ctgccacagt ttatagcgaa atagttactt attctgggca catgaacatt gccgattccc
    29041 ctgtaagtcg ggtaataaca acactcatat aaagaataag gtttttacaa ccaaaaaaga
    29101 aggtcgttat gaatcgtttt attatggcca acagtcagca atgtctgggt tgtcatgctt
    29161 gtgaaatcgc ctgtgtcatg gctcacaatg atgagcaaca tgtcctgagc caacaccatt
    29221 ttcatccccg aattacggtt atcaaacatc agcagcaacg tagtgcagtg acctgtcacc
    29281 attgtgaaga tgcgccctgc gcccgtagct gccctaatgg cgcaatcagc cacgttgatg
    29341 acagcattca ggtcaatcag caaaagtgta ttggctgtaa atcctgcgtg gtggcctgtc
    29401 cgtttggtac gatgcaaatc gtcctgacac ccgtcgcggc aggaaaagta aaagccacgg
    29461 cgcataaatg cgacctttgt gcagggcgcg aaaacggtcc tgcctgtgtc gagaattgcc
    29521 cggcggacgc gctgcaactg gtcactgacg tcgcactctc cggcatggcg aaatcccgcc
    29581 gcttgcgcac cgcacgtcag gagcatcaac cgtggcatgc cagtaccgct gcacaagaaa
    29641 tgccggtaat gagtaaagtc gaacaaatgc aggcaacgcc cgcgcgtggc gagccggata
    29701 aactggcgat tgaagcgcgc aaaaccggtt ttgatgaaat ttatctgcca ttccgtgccg
    29761 atcaggcaca acgggaagcc tcgcgctgcc ttaaatgcgg cgagcacagc gtttgtgaat
    29821 ggacctgccc gctgcataac catattccgc agtggattga actggtgaaa gccggaaaca
    29881 tcgacgccgc cgtcgagctt tctcacctga ccaacaccct gccggaaatt accggacgcg
    29941 tttgtccgca ggaccgtttg tgtgaaggtg cctgtactat tcgcgatgag cacggcgcgg
    30001 taactatcgg caacattgaa cgctacattt cagatcaggc gttggcgaaa ggttggcgtc
    30061 ctgacttaag ccatgtcacc aaagtggaca agcgggtggc gattatcggt gcgggtccgg
    30121 cagggctggc ctgtgcggat gttctgaccc gcaatggcgt ggcggtgacg gtgtacgatc
    30181 gccatccaga aattggtggc ttgctcactt tcggcattcc ttctttcaaa ctggataaat
    30241 ccctgctggc acgccgtcgg gaaatcttca gcgcgatggg gattcacttc gaactcaatt
    30301 gtgaagtggg taaagatgtc tctttggatt cgcttttgga acaatacgac gcggtcttcg
    30361 ttggcgtagg cacttaccgt tccatgaaag cgggtttacc caatgaagat gcgccgggcg
    30421 tttatgacgc gctgccgttc ctcatcgcca acactaaaca ggtgatggga ctcgaagagc
    30481 taccggaaga gccgtttatc aataccgccg gactcaacgt cgtggtactg ggcggcggcg
    30541 acaccgcgat ggactgtgtg cgtaccgcgc tgcgccacgg cgcgagtaac gtcacctgcg
    30601 cttatcgtcg tgatgaagct aacatgccag gctcgaagaa agaggtgaag aacgcccgcg
    30661 aagagggggc caacttcgaa tttaacgtcc agccggtggc gcttgagctg aatgagcaag
    30721 gtcacgtttg cggaattcgt ttcctgcgca cgcgtcttgg cgagccggat gcccaggggc
    30781 gccggcgact tgtaccgatt gagggcagtg aatttgtcat gccagccgat gcggtgatta
    30841 tggtgtttgg cttcaatcca cacgggatgc cgtggctgga gtcgcacggt gtaacggtag
    30901 acaaatgggg ccgcatcatc gcggatgtgg aaagccagta ccgttaccag accaccaatc
    30961 cgaaaatctt cgccggtggc gacgccgtgc gtggtgcgga tctggtggtt accgcaatgg
    31021 cagaaggacg tcatgcggca caggggatta ttgactggct gggggtaaaa tcagtcaaat
    31081 ctcactgata gcctgcgcag acaaacccga cttcacagcg taatataatt gttcatttcg
    31141 cgctgtggag tcggtatgac gcaacaaatc accctcatta aagacaaaat tctctccgat
    31201 aactatttca ccctgcacaa cattacttac gatctcacgc gcaaagatgg cgaagttatc
    31261 cgccataaac gtgaagttta cgatcgcggt aatggtgcga cgatcctcct gtacaacgcg
    31321 aagaaaaaga ccgtggttct gattcgtcag ttccgtgtcg ctacctgggt taatggcaat
    31381 gaaagcgggc agctgattga aacctgcgcc gggctgctgg ataacgacga accggaagtg
    31441 tgcattcgta aagaagcgat tgaagagacg ggctatgaag tgggcgaagt gcgcaaatta
    31501 tttgaactgt atatgtcgcc tggcggtgtg actgagctaa tccacttttt tatcgccgaa
    31561 tacagtgaca atcagcgcgc taacgccggt ggcggtgttg aagatgaaga tattgaagtg
    31621 cttgagctgc cgttcagcca ggcgctggag atgatcaaaa ccggcgagat acgtgacggt
    31681 aagacggtgt tattgcttaa ctatttgcaa acgtcacatt taatggactg aaaaataaca
    31741 ataatatttc gttgtttatt attggctcaa tccgacaaat ctgattgagc cgcgctacct
    31801 gcgcaacgaa gatacgactt gtgctgtttg tttgaacttc tggggtcgta ccgtccatgc
    31861 gctatcgcat tttccttctc ttttttttcg ctttgttgcc gacgtctttg gtgtgggcgg
    31921 cactagcgca acgggcgttt tccgactggc aggtcacctg taataaccaa aacttctgcg
    31981 tggcgcgcaa tacgggcgat cataatggac tggtgatgac cctgagccgc agcgccgggg
    32041 cgcataccga tgccgtttta cgtattgagc gcggcgggtt gaagtcgccg gatgcgtctg
    32101 aaggagagat agcgccacgg ctgctgttag atggtgagcc gttggcgtta agtggtgaca
    32161 agtggcggat ttcaccatgg ttattagtaa ccgatgatac ggcaaccatc accgcgtttt
    32221 tgcagatgat tcaggaaggg aaggcgatca ccttacgcga cggcaatcag accatttctc
    32281 tgagtggctt aaaagcggcg ttgttgttta ttgatgctca gcaaaagcgc gttggcagtg
    32341 aaaccgcgtg gatcaagaaa ggggacgaac cgccgctcag cgtaccgccc gcgcctgcgc
    32401 tgaaagaggt cgcggtggtt aacccaacgc cgacgccact ctcactcgaa gaacgcaacg
    32461 atttgctgga ttatggaaac tggcggatga atggtctgcg ctgctcgctt gatccgttac
    32521 gtcgtgaggt gaatgtcact gcgctgactg atgataaagc gctgatgatg atcagctgtg
    32581 aggcaggggc ctataacacc attgatttgg catggattgt gtcgcgtaaa aagccgctag
    32641 cctcgcgccc ggttcggttg cgtttgccgt tcaacaacgg gcaggagacg aatgaactgg
    32701 aactgatgaa cgcaacattt gatgagaaat cgcgtgaact ggtgacctta gcgaaagggc
    32761 gcggattaag cgattgtggc attcaggcgc gctggcgctt tgacggtcag cgttttcgcc
    32821 tggtgcgtta cgccgcagaa cccacctgcg ataactggca tgggccagac gcctggccca
    32881 cgttgtggat cacccgttaa acaaaatggc ggggacatgt aggccggata agacgctgta
    32941 ggccggataa gacgcgtcag cgtcgcatcc ggcaatcagc atccggcgat cactatcagg
    33001 cacctttcac tcccagcacc ttatgcgctt ttgccacaat attctcggcg gtaaagccaa
    33061 agaacgggaa cagcttatcc gccggagcag attccccata acccgtcatc ccgacaattg
    33121 cccctttcag accaacatac ttgtaccagt aatcggcaat acctgcttcc accgccacgc
    33181 gagccgcaac gttagaaggc aacaccgact cccgatattc ctcatcctgg gcgtcgaaaa
    33241 tatcggtcga gggcagggaa accacgcgaa cattgcgacc ttctcctgct aatttctccg
    33301 cagcttgcag ggtgatttcc atctctgaac cggtggcaat cagaataata tctggcttac
    33361 cgccgctgtc tttcagcaca taaccgccac gagtaatctc tttaacctga tccggcgtac
    33421 gttccacctg ggccagattc tgccttgaga ggatcagtgc cgtcggtccg ttgtggcgct
    33481 caaccgccag cttccagccc accgccgctt ccacctgatc gcacggtcgc caggtgctga
    33541 aatttggcgt taagcgcagg ctggccagtt gctcaacagc ctggtgcgtc ggaccatctt
    33601 cgcccaggcc aattgagtcg tgggtataaa ccataatctg ccgcgctttc atcagtgccg
    33661 ccatccgcgc ggcgttacgg gcgtattcaa caaacatcag gaacgtcgcg gtatacggca
    33721 caaagccgcc gtggtgcgcg atgccgttgg cgatagcggt catgccaaat tcacgcaccc
    33781 cgtagtgaat gtagttgccc gccggatctt ccttcagcga aacagaacct ttccagatgg
    33841 tcaggttgct gggagccaga tccgccgaac cgccgagcag ctccggtagc aaggggccat
    33901 aagcgttaag cgtattttgc gaagccttac gggtggcgat tttcgccgga ttcgcctgca
    33961 actcattgat atacttctga gtcgttttct cccagtcctt cggtaaacca ccgctcatcc
    34021 gacgggtaaa ctcttctgcc agttgcggat gagccttttt ataggcggca aacttctcat
    34081 tccacctctg ctgcgctttt tcgccttttt cgcgggcatc ccaggcgtga taaatatctt
    34141 tagggatctc aaatggcgga tggtgccagc ccagtttttg ccgtgccagc gccacttctt
    34201 cttcccccag cggtgcgccg tgcgcctctt ccttacctgc tttattcggc gatccaaagc
    34261 caatcaccgt acgacagata atcagcgacg gcttatcttt cacgctttgc gcttcaagga
    34321 tcgcttcctt caccgcctgc ggatcgtgac cgtcgatttc atggatcaca tgccagtgat
    34381 aggcttcaaa acgttttgcc gtatcgtcgg taaaccagcc ttctgtttca ccgtcgatgg
    34441 aaataccgtt gtgatcgtaa aaaccaatca gcttgcccag tcccagcgtg cctgccagcg
    34501 aacagacttc gtgggaaata ccttccatca ggcagccgtc gcccataaac acataggtga
    34561 agtgatcgac gatctcatgg tctggctggt taaactgcgc cgccagtgta cgctctgcta
    34621 tcgccagccc gacggcgttc gccaaacctt gtccaagtgg accggtggtg gtttcaacgc
    34681 ctggcgtata accaatctcc gggtggcctg gggttttcga atgcagttga cgaaagttct
    34741 tcagctcttc cagcggcagg tcgtaaccgg tcagatgcag caaactgtag agcagcatcg
    34801 acgcgtgacc gttggaaaga ataaagcggt cgcgatcata ccaggttggg tcggtagggt
    34861 tatgtttaag aaaatcgttc cacagcactt cggcaatatc agccatgccc atcggcgcgc
    34921 cgggatgacc agaattggct ttttgtaccg catccatact gagtgcgcga atcgcattgg
    34981 caaggtcttt tcgggacata taacactccg tggctggttt atagtttggc ggcaagaaga
    35041 tcttccagtt tgcgttgatc aacggcgaac agacgaatgc cttcagacag tttttctacc
    35101 gccatcgcat actgattgtg ctcccaacgg aactccgctt cgctcatggg agctgggcgt
    35161 gggaacgtct gagaaggtgg gattaattta cgtaccactg gcgaaacttt ttcctgcagc
    35221 tccttcagca aattcggtgc gatagtcagt cgatcgcagc cggttaaggc gaggatttgt
    35281 tcggtgcgac ggaagctcgc gcccatcaca atggtttcat agtggtgttg cttatagtag
    35341 tcgtagatat tgcgcaccga tttaacgccc ggatcttctt ccaccacata cgggtccatc
    35401 ggcttgcgtg cctgatacca gtcataaata cgcccgacaa acggcgaaat cagaaaaacg
    35461 cctgcttccg cacaggcccg tgcctgtgca aaagaaaaca gcagcgtcag gttgcagtta
    35521 ataccttctt tttccagctc ttctgccgcg cgaattcctt cccaggtcga agccagcttg
    35581 atcagaatgc gtgatttctc aacgccttgt tgctgataca agtccaccag atggcgcgcc
    35641 ttctcaatac tcttttcttt atcaaaagag aggcgtgcat caacttctgt tgacacgcga
    35701 ccgggtacga ttttgaggat ttcagcaccg aaattgaccg ccagtttgtc acacgctgcg
    35761 accacctgtt gttcctgggt cttgccattt tttttacccc aggcgatagc atcgtctatt
    35821 aaatgctcat attgtgataa tccggcagcc ttgagtaaca gcgaaggatt ggtggtggca
    35881 tcctggggat gataatggcg aatggactca atatcgccgc tgtctgccac gacagtggtg
    35941 aactgtttga tgccgtctaa ctcgttcata ggaaatactc cttgaaaagt aaagtgttag
    36001 atgagtgcgt taattcacac ttctgagaaa tttcgctaaa cgcatcaaaa aagcatagca
    36061 gacaggcatg gtattgctgg attaagcagg taacatcagt gttataggat tattaccaaa
    36121 acattatatt aatggtgaga gtttggactt gctcaaagtc tgtagactcc ggcaaggtaa
    36181 taatgtgcgc cacgttgtgg gcagggggtg ttgcccacac actttatttg tgaacgttac
    36241 gtgaaaggaa caaccaaatg gatgaccagt taaaacaaag tgcacttgat ttccatgaat
    36301 ttccagttcc agggaaaatc caggtttctc caaccaagcc tctggcaacg cagcgcgatc
    36361 tggcgctggc ctactcaccc ggcgttgccg caccttgtct tgaaatcgaa aaagacccgt
    36421 taaaagccta caaatatacc gcccgcggta acctggtggc ggtgatctct aacggtacgg
    36481 cggtgctggg gttaggcaac attggcgcgc tggctggcaa accggtgatg gaaggcaagg
    36541 gcgttctgtt taagaaattc gccgggattg atgtatttga cattgaagtt gatgaactcg
    36601 acccggacaa atttattgaa gttgtcgctg cgctcgaacc gaccttcggc ggaatcaacc
    36661 tcgaagacat taaagcgcca gaatgtttct atattgaaca gaaactgcgc gagcggatga
    36721 atattccggt attccacgac gatcagcacg gcacggcaat catcagcact gccgccatcc
    36781 tcaacggctt gcgcgtggtg gagaaaaaca tctccgacgt gcggatggtg gtttccggcg
    36841 cgggtgccgc agcaatcgcc tgtatgaatc tgctggtagc gctgggtctg caaaaacata
    36901 acatcgtggt ttgcgattca aaaggcgtta tctatcaagg ccgtgagcca aacatggcgg
    36961 aaaccaaagc cgcatatgcg gtggtggatg acggcaaacg taccctcgat gatgtgattg
    37021 aaggtgcgga tattttcctg ggctgttccg gcccgaaagt gctgacccag gaaatggtga
    37081 agaaaatggc tcgggcacca atgattctgg cgctggcgaa tccggaaccg gaaattctgc
    37141 cgccgctggc gaaagaagtg cgtccggatg ccatcatctg caccggtcgt tctgactatc
    37201 caaaccaggt caacaacgtc ctttgcttcc cgttcatctt ccgtggcgcg ctggacgttg
    37261 gcgcaaccgc catcaacgaa gagatgaaac tggcggcggt acgcgcgatt gcagaactgg
    37321 cccatgcgga acagagcgaa gtggtggctt cagcgtatgg cgatcaggat ctgagctttg
    37381 gtccggaata catcattcca aaaccgtttg atccgcgctt gatcgttaag atcgctcctg
    37441 cggtcgcgaa agcggcgatg gagtcgggcg tggcgacgcg tccgattgct gatttcgacg
    37501 tctacatcga caagctgact gagttcgttt acaaaaccaa cctgtttatg aagccgattt
    37561 tctcccaggc tcgcaaagcg ccgaagcgcg tagttctgcc ggaaggggaa gaggcgcgcg
    37621 ttctgcatgc cactcaggaa ctggtaacgc tgggactggc gaaaccgatc cttatcggtc
    37681 gtccgaacgt gatcgaaatg cgcattcaga aactgggctt gcagatcaaa gcgggcgttg
    37741 attttgagat cgtcaataac gaatccgatc cgcgctttaa agagtactgg accgaatact
    37801 tccagatcat gaagcgtcgc ggcgtcactc aggaacaggc gcagcgggcg ctgatcagta
    37861 acccgacagt gatcggcgcg atcatggttc agcgtggcga agccgatgca atgatttgcg
    37921 gtacggtggg tgattatcat gaacatttta gcgtggtgaa aaatgtcttt ggttatcgcg
    37981 atggcgttca caccgcaggt gcaatgaacg cgctgctgct gccgagtggt aacaccttta
    38041 ttgccgatac ctacgtcaat gacgaaccag acgcagaaga gctggcggag atcaccttga
    38101 tggcggcaga aactgtccgt cgtttcggta ttgaaccgcg cgttgctctg ttgtcgcact
    38161 ccaactttgg ttcttctgac tgcccgtcgt cgagcaaaat gcgtcaggcg ctggaactgg
    38221 tcagggaacg tgcgccagaa ctgatgattg atggtgaaat gcacggcgat gcggcgctgg
    38281 tggaagcgat tcgcaacgac cgtatgccgg acagcccttt gaaaggttct gccaatattc
    38341 tggtgatgcc gaacatggaa gctgcccgca ttagttacaa cttactgcgc gtttccagct
    38401 cggaaggtgt gactgtcggt ccggtgctga tgggcgtggc gaaaccggtt cacgtgttaa
    38461 cgccgatcgc atcggtgcgt cgtatcgtca acatggtggc gctggccgtg gttgaagcgc
    38521 aaacccaacc gctgtaattc ttttttaact ctcacgctta ccctgaatat tcagggtaag
    38581 cggtttggct gcaatatatt agtaaagctt attactgagt ttgcgaataa taaaaaaagc
    38641 agtctatata atatctcgat attattttat ttatattcat gcgttgcata tgaaagttta
    38701 tgcaccacag cgaatatctc tccattctta gtgatctacc tcacctttta aacgcgcttg
    38761 ccgaattttg ttatttactc tgacgaaaaa ttgtcacgat acacgaaagt ttttcacagg
    38821 cggcgactca tggataaaga acgcatcatt caggaatttg tgcccggcaa acaggtcaca
    38881 ctggcgcatc tcatcgcgca tcccggtgag gaactggcga agaaaatcgg cgttccggat
    38941 gcgggcgtaa ttggcattat gacgctaact cccggcgaaa cggcgatgat cgtcggtgat
    39001 ttagcgctga aagccgctga tgtgcatatc ggttttctcg accgcttcag cggtgcattg
    39061 gtgatttatg gctcggtggg cgcagtagaa gaagcgttat cacaaaccgt cagcggttta
    39121 ggtcgcttat taaattatac gctttgtgaa atgaccaaaa gttaatacga ggcattcatg
    39181 aaacgaattg cgtttgtggg tagcgtcggg gcaggaaaaa caacgttatt taatgcgttg
    39241 caggggaatt ataccctcgc cagaaaaaca caggccgtgg aatttaatga taagggcgat
    39301 attgatacgc cgggtgaata ttttagccat ccccgctggt atcacgcctt aattaccacg
    39361 ctacaggatg tagacatgct gatctatgtc catggcgcaa atgacccgga aagtcggttg
    39421 cctgccgggt tgctggatat tggcgtcagc aaacggcaaa tcgccgtcat cagtaaaacg
    39481 gacatgccag atgctgacgt cgccgcaacg cgaaagttat tgcttgagac gggtttcgaa
    39541 gagccgattt tcgagctaaa cagccacgac ccgcaaagcg tgcagcaact ggtggattat
    39601 ctggcatctc tcaccaaaca ggaggaagca ggtgaaaaaa ctcatcacag cgaatgatat
    39661 tcgtgaagca cacgcgcgcg gcgaacaggc aatgtccgtg gttctgcgcg ccagcattat
    39721 taccccggaa gcccgtaaag tggcggatct actgggtttt accatcaccg aatgtgatga
    39781 atcgatcccg gtaacggcgt ccgtgcccgc cagcgtgcca gccgacaaaa ccgaaagcca
    39841 gcgcatccgc gaaaccatca ttgcccagct gccggaaggc cagtttaccg aaagcctggt
    39901 cgcacagctg atggaaaaag tgatgaagga aaaacagtcg ctggagcagg gcgcaatgca
    39961 gccgagcttc aaatcggtga ccggcaaagg cggcatcaaa gtgattgatg gcagcagcgt
    40021 caagtttggt cgttttgatg gcgcacaacc gcattgcgtg ggcttgactg atcttgtgac
    40081 cggagacgac ggcagcagca tggccgccgg ttttatgcag tgggagaacg cattcttccc
    40141 gtggaccctg aactacgacg aaatcgacat ggtgctggaa ggggagctcc atgttcgcca
    40201 cgaaggcgaa accatgatcg ccaaagcagg cgacgtgatg tttatcccga aaggctccag
    40261 catcgaattt ggtacgacat ccagcgtgaa attcctgtat gtcgcctggc cggctaactg
    40321 gcaatcccta tgaaagattt catcaccgaa gcatggctaa gagcgaacca tacgctcagc
    40381 gaaggcgcag agatccatct ccctgcggac agtcgcctga cgccctctgc ccgggagttg
    40441 ctggaaagcc gccatctgcg catcaagttt attgacgagc agggccgcct gtttgttgac
    40501 gatgaacagc agcagccgca gcccgttcat gggctgacca gtagcgatga acatccgcag
    40561 gcgtgctgcg aactgtgccg ccagccggtg gcaaaaaagc cggatacgct gactcacctg
    40621 tcggcggaaa aaatggtcgc caaaagcgat ccgcgtctgg gttttcgcgc ggttctcgac
    40681 agcaccattg cgctggcagt gtggctgcaa attgaactgg cggaaccgtg gcagccgtgg
    40741 ctggcggata tccgttcgcg tcttggcaac attatgcgcg ccgatgcgct gggggaaccg
    40801 ctgggctgcc aggcgattgt cgggctttct gacgaagatc tgcatcgact ttctcaccag
    40861 ccgctgcgct atctcgacca cgatcatctg gtgccggaag ccagccacgg gcgcgatgcg
    40921 gcgttgctca atctgctacg taccaaagtg cgggaaaccg aaaccgtagc ggcgcaggtg
    40981 tttatcaccc gcagctttga agtattacgc ccggacatct tgcaggcgct gaaccgcctt
    41041 tcgagcacgg tctacgtaat gatgatcctg agcgtaacga agccgccgct aacggtgaag
    41101 cagatccaac aacgactggg ggaaacgcaa tgattattga acgttgtcgt gaactggcgt
    41161 tgcgagcgcc cgccagagtg gtttttccgg atgcgttaga tcaacgtgtg ctgaaagctg
    41221 cgcaatattt acatcaacaa ggtctggcaa cgcccattct ggtcgctaat ccgtttgaac
    41281 ttcgtcagtt tgcgctcagt cacggcgtgg cgatggacgg gctacaggtg attgatccgc
    41341 atggcaacct cgcaatgcgg gaagaatttg ctcatcgctg gctggcccgc gcgggcgaaa
    41401 aaacgccgcc ggatgcgctg gaaaaactta ccgatccgct gatgttcgcc gcagcaatgg
    41461 tcagcgccgg taaagcggat gtctgtatcg cgggcaacct ctcttccacg gcgaatgtgc
    41521 tgcgtgccgg attacgtatt atcggcttgc agccaggctg taaaacgctc tcatccattt
    41581 tcctgatgct gccacagtac agcggtccgg cgttgggctt tgccgattgc agcgtggtgc
    41641 cacagccgac ggcggcgcaa ctggcggata tcgcgcttgc cagtgccgaa acctggcgcg
    41701 ccctcaccgg agaagagccg cgcgtggcga tgctgtcgtt ttccagtaac ggtagcgccc
    41761 gtcacccctg cgttgccaac gtccagcagg cgacagaaat cgtccgtgag cgcgcaccaa
    41821 agctggtggt tgatggcgag ttgcagtttg acgccgcctt cgtgccggaa gtggcggcgc
    41881 aaaaagcgcc tgccagcccg ctacagggca aagccaatgt gatggttttt ccgtcgctgg
    41941 aagccggaaa tattggctac aaaatcgcac aacgactcgg cggatatcgt gccgtcgggc
    42001 cattgataca aggacttgcc gcgccgatgc acgatctctc tcgtggttgt agcgtgcagg
    42061 aaattatcga gctggcgctg gtggcagctg tgccgcgtca gacagaagtg aaccgcgaaa
    42121 gcagtttaca aacactggtt gaatgaacgg tcccgttctg gaccctgata aagaggaaag
    42181 cacaatggaa gcattaggaa tgatcgaaac ccggggcctg gttgcactga ttgaggcttc
    42241 tgacgcgatg gttaaagctg cgcgcgtcaa gctggttggt gttaagcaga ttggcggcgg
    42301 cctgtgtact gccatggttc gtggtgatgt tgcagcctgt aaagccgcga ccgatgcagg
    42361 tgccgcagcg gcacagcgta tcggcgagct ggtatctgta cacgttatcc cgcgtccgca
    42421 cggcgacctg gaagaagtct tcccgatcgg cctgaaaggc gatagcagca acctgtaatc
    42481 accctgtgtg acgtcgattc gggcgtcacg ctctctcctt tttcatttta ccttctgcgg
    42541 agggtagggg gagcttttac ctgaaatacg ccacggaggc gggtatgaaa ctggcagtcg
    42601 tcactggaca aattgtttgt accgtacgcc atcacggact ggcgcatgac aaattgctga
    42661 tggtggaaat gattgatcca caaggtaatc ctgacgggca atgcgccgtc gccatcgaca
    42721 atattggcgc gggaaccggg gagtgggtgt tactggtgag tggcagttcc gcccgccagg
    42781 cgcataaaag cgaaacgtca ccggtcgatc tgtgcgtgat tggcattgtc gatgaggtgg
    42841 tgtctggcgg tcaggtaatt ttccacaaat aaggcagaac atcatgaatc aacaggatat
    42901 tgaacaggtg gtgaaagcgg tactgctgaa aatgcaaagc agtgacacgc cgcccgccgc
    42961 cgttcatgag atgggcgttt tcgcgtccct ggatgacgcc gttgcggcag ccaaagtcgc
    43021 ccagcaaggg ttaaaaagcg tggcaatgcg ccagttagcc attgctgcca ttcgtgaggc
    43081 aggcgaaaaa cacgccagag atttagcgga acttgccgtc agtgaaaccg gcatgggccg
    43141 cgttgaagat aaatttgcca aaaacgtcgc tcaggcgcgc ggcacgccag gcgttgagtg
    43201 tctctctccg caggtgctga ccggcgacaa cggcctgact ttaattgaaa acgcgccctg
    43261 gggcgtggtg gcttcggtaa ctccgtccac taacccggcg gcaaccgtaa ttaataacgc
    43321 catcagcctg attgccgcgg gcaacagcgt catttttgcc ccgcatccgg cggcgaaaaa
    43381 agtctcccag cgggcgatca cgctgctcaa ccaggcgatt gttgccgcag gcgggccgga
    43441 aaacttactg gttactgtgg caaatccgga tatcgaaacc gcgcagcgtt tgttcaagtt
    43501 tccgggcatc ggccttctgg tggtgaccgg cggcgaagcg gtagtagaag cggcgcgtaa
    43561 acacaccaat aaacgtctga ttgccgcagg cgctggcaac ccgccggtag tggtggatga
    43621 aaccgccgac ctcgcccgtg ccgctcagtc catcgtcaaa ggcgcttctt tcgataacaa
    43681 catcatttgt gccgacgaaa aggtactgat tgttgttgat agcgtagccg atgaactgat
    43741 gcgtctgatg gaaggccagc acgcggtgaa actgaccgca gaacaggcgc agcagctgca
    43801 accggtgttg ctgaaaaata tcgacgagcg cggaaaaggc accgtcagcc gtgactgggt
    43861 tggtcgcgat gcggcgaaaa tcgcggcggc aatcggcctg aacgtgccgc aagaaacgcg
    43921 tttgctgttt gtggaaacca ctgcagaaca tccgtttgcc gtgaccgaac tgatgatgcc
    43981 ggtgctgccc gtcgtgcgcg tcgccaacgt ggcggatgcc attgcgctgg cggtgaaact
    44041 ggaaggcggt tgccaccaca cggcggcaat gcactcgcgc aacatcgaaa acatgaacca
    44101 gatggcgaat gctattgata ccagcatttt cgttaagaac ggaccgcgca ttgccgggct
    44161 ggggctgggc ggggaaggct ggaccaccat gaccatcacc acgccaaccg gtgaaggggt
    44221 aactagcgtg cgtacgtttg tccgtctccg tcgctgtgta ttagtcgatg cgtttcgcat
    44281 tgtttaagga gtaacagatg gcgcacgacg aacaatggct caccccacgt ctgcaaacgg
    44341 cggcaacgct gtgtaaccag acgcccgccg cgacggaatc tccgctgtgg ctgggggtgg
    44401 atctgggcac ctgcgatgtg gtgtcgatgg ttgtcgaccg cgacggtcag tcggtggcgg
    44461 tatgcctcga ctgggccgat gtggtccgcg acggcatcgt ctgggatttc ttcggcgctg
    44521 tcaccattgt tcgtcgccat ctcgacacgc tcgagcagca attcggtcgc cgttttagcc
    44581 atgcggcgac ttcatttcct ccaggtacgg acccgcgtat ttccatcaac gtgctggagt
    44641 ctgctggact ggaagtgagc catgtgctgg atgagccaac cgcagttgcg gatctgctgc
    44701 aactggacaa cgccggtgta gtggatatcg gcggcggcac taccggcatt gccatcgtga
    44761 aaaagggcaa ggtgacgtac tcggcggatg aagcaaccgg cggacatcac atctctctga
    44821 ctctcgccgg aaatcgccgt atttcgctgg aagaggcgga gcagtacaag cgcggtcacg
    44881 gtgaagagat ttggcctacg gtgaaaccgg tgtacgaaaa aatggcggac attgtcgccc
    44941 ggcatattga agggcagggc attactgatt tatggctggc gggcggctct tgtatgcaac
    45001 cgggcgtggc ggaactgttt cgcaaacaat tcccggcgtt acaggtgcat ttaccgcagc
    45061 acagtttgtt tatgaccccg ctggcgatcg ccagtagtgg gcgagagaaa gcggaggggc
    45121 tatatgcaaa atgaattgca gaccgcgctc tttcaggcgt tcgataccct gaatctgcaa
    45181 cgggtaaaaa catttagcgt tccaccggtg acgctttgcg gtccgggctc ggtgagcagt
    45241 tgcggacagc aagcgcaaac gcgtgggctg aaacatctgt tcgtgatggc agacagcttt
    45301 ttgcatcagg cagggatgac cgccgggctg acgcgcagcc tggctgttaa aggcatcgcc
    45361 atgacgctct ggccatgtcc ggtgggcgaa ccgtgcatca ccgacgtgtg tgcagccgtg
    45421 gcgcagttgc gtgagtcagg ctgtgatggg gtgatcgcat ttggcggcgg ctcggtgctg
    45481 gatgcggcga aagccgtggc gttgctggtg acgaacccgg atagcacgct ggcagagatg
    45541 tcagaaacca gcgttctgca accgcgcttg ccgctgattg ccattccaac gaccgccgga
    45601 accggctctg aaaccaccaa tgtaacggtg attatcgacg cggtgagcgg gcgcaagcag
    45661 gtgttagccc atgcctcgct gatgccggat gtggcgattc tcgacgccgc gttgaccgaa
    45721 ggtgtgccgt cgcatgtcac ggcgatgacc ggcattgatg cgttaaccca tgccattgaa
    45781 gcatacagcg ccctgaacgc tacaccgttt accgacagtc tggcgattgg tgccattgcg
    45841 atgattggca aatcgctgcc gaaagcggtg ggctacggtc acgaccttgc cgcgcgcgag
    45901 agcatgttgc tggcttcatg tatggcggga atggcgtttt ccagtgcggg tcttgggttg
    45961 tgccacgcga tggcgcatca gccgggcgcg gcgctgcata ttccacacgg tctggcgaac
    46021 gccatgttgc tgccaacggt gatggaattt aaccggatgg tttgtcgtga acgctttagt
    46081 cagattggtc gggcactgcg aactaaaaaa tccgacgatc gtgacgctat taacgcggta
    46141 agtgagctga ttgcggaagt tgggattggt aaacgactgg gcgatgttgg tgcgacatct
    46201 gcgcattacg gcgcatgggc gcaggccgcg ctggaagata tttgtctgcg cagtaacccg
    46261 cgtaccgcca gcctggaaca gattgtcgga ctgtacgcgg cggcgcaata aatgccggat
    46321 gcgacgcttg ccgcgtctta tccagcctac gggattgcac atgtagggcg gataaggcgt
    46381 tcacgccgca tccgccagtg gcgcggtgca attgccggat gcgatgcttg ccgcgtctta
    46441 tccggcctac gggattgcac atgtagggcg gataaggcgt tcacgccgca tccgccagtg
    46501 gcgcggtgca attgccggat gcgacgcttg ccgcgtctga tccggccttc gggattgcac
    46561 atgtagggcg gataaggcgt ttacgccgca tccgccaata aataacaact aacagggagt
    46621 aaaggcgatg ggaattaacg aaatcatcat gtacatcatg atgttcttta tgctgatagc
    46681 tgccgtagac aggatcctgt cgcagttcgg cggttctgct cgtttcctcg gtaagttcgg
    46741 taaaagtatc gaaggatcag gcggtcagtt cgaagaaggc tttatggcaa tgggcgctct
    46801 gggtctggcg atggtcggta tgaccgcgct ggcaccggta ctggctcacg tactcgggcc
    46861 ggtaattatc ccggtttacg aaatgctcgg cgcaaacccg tcaatgttcg ccggaacgct
    46921 gctggcgtgc gatatgggcg gcttcttcct cgccaaagag ctggcgggcg gcgacgtagc
    46981 agcgtggcta tactctgggt taattcttgg gtcgatgatg gggccaacga ttgtgttttc
    47041 cattccggtg gcgctcggca ttatcgaacc ttctgaccgt cgttatctgg cgctcggcgt
    47101 gctggcgggc attgtgacca ttccgattgg ctgtattgct ggtggtctgg ttgctatgta
    47161 ctccggtgtg cagatcaacg gtcagccggt ggaattcact ttcgccctga tcctgatgaa
    47221 catgatcccg gtgatcattg ttgcgattct ggtggcgctg gggctgaaat tcatcccgga
    47281 aaaaatgatc aacggcttcc agatcttcgc caaattcctc gttgcattga tcaccctcgg
    47341 tcttgctgcc gcggtagtta agtttctgct tggctgggaa ctgatccccg gtctggaccc
    47401 tatctttatg gcccctggcg ataaacccgg tgaagtgatg cgcgccattg aagttatcgg
    47461 ttctatctcc tgcgttctgt taggggcgta tccaatggtg ctgctgctga cccgctggtt
    47521 tgaaaaaccg ctgatgagcg tcggtaaagt gctgaatatg aacaacatcg cggcagccgg
    47581 catggtggca acgcttgcca acaacatccc gatgttcggc atgatgaagc agatggatac
    47641 ccgcggcaaa gtcatcaact gcgccttcgc cgtttccgct gctttcgccc tgggcgacca
    47701 cttaggcttc gccgctgcca acatgaacgc catgatcttc ccgatgattg tcggcaagtt
    47761 gatcggcggc gtaacggcga ttggcgtggc gatgatgctg gtgccaaaag aagacgcgac
    47821 cacggctaaa accgaagcgg aggcacaatc gtgaacactc gccagctatt gagcgtcggt
    47881 atcgatatcg gcaccaccac cacccaggtg attttctccc acctggagct ggttaaccgt
    47941 gcggcggtgt cgcaggtgcc gcgctacgaa ttcattaaac gcgaaattag ctggcaaagc
    48001 ccggtgttct ttacccctgt cgataaacag ggtggtttaa aagaagcgga actgaaaacc
    48061 ttaatcctcg agcaatatca ggctgcgggc attgcgccgg aaagcgttga ttctggtgcc
    48121 atcatcatca ccggtgaaag cgcgaaaacc cgcaatgctc gtccggcggt gatggtgctc
    48181 tcccagtcgc tgggcgattt tgtcgttgcc agcgccgggc cgcacctcga atccgtcatt
    48241 gccggtcacg gtgccggggc acaaaccctt tctgaacaac ggctgtgtcg ggtactgaat
    48301 attgacatcg gcggtggcac tgcgaactac gccctgttcg atgccggaaa aatcagcggc
    48361 actgcctgcc tcaacgtcgg tggtcgcctg ctggaaaccg acagccaggg gcgcgtggtt
    48421 tacgctcata aaccggggca gatgattgtg gatgagtgct tcggcgcagg caccgacgcc
    48481 cgttcgctga ccggcgcgca gctggtgcag gttacccggc ggatggcaga actgattgtc
    48541 gaagtgattg acggaacgct ctcgccgctc gcgcaggcat tgatgcaaac cggtttgctg
    48601 cccgcaggtg ttacgcccga aatcattacg ctttctggcg gcgtgggcga atgttatcgc
    48661 caccagcccg ccgacccgtt ctgttttgcc gatattggcc cgctgctggc aacggcgctg
    48721 catgaccatc cgcgcctgcg tgagatgaac gtacagtttc cggcgcaaac cgtgcgcgcc
    48781 acggtgattg gcgcgggcgc gcataccctt tcgctctctg gcagcacaat ctggcttgag
    48841 ggcgtacaac tgccgctgcg caatttgccg gtggcgatcc cgattgatga aacggatctg
    48901 gtgagtgcct ggcaacaggc gctgcttcag ctggatcttg atcccaaaac tgacgcgtac
    48961 gtgctggcgc ttcccgcctc gctgcctgtg cgttacgccg cggtactgac ggtcatcaac
    49021 gcgctggtcg atttcgtcgc gcgttttcca aatccgcatc ccctgctggt ggtggccggg
    49081 caggactttg gtaaagctct gggcatgttg ttgcgcccac agctacaaca actcccgttg
    49141 gcagtcattg acgaagtgat tgtccgcgcg ggggactata tcgacattgg tacgcctctt
    49201 tttggcggat cggttgtgcc ggtgacggtg aaatcactcg catttccttc ctgagggaac
    49261 gacttatgaa actaaagacc acattgttcg gcaatgtata tcagtttaag gatgtaaaag
    49321 aggtgctggc taaagccaac gaactgcgtt cgggggatgt gctggcgggc gttgcagcgg
    49381 caagctcaca ggagcgcgtg gcggcaaagc aggtgttgtc ggaaatgacc gtagcggaca
    49441 tccgcaataa tccggtgatt gcctatgaag atgactgcgt gacgcggctg attcaggacg
    49501 atgttaacga aacggcctac aaccagatta aaaacaggag catcagcgaa ctgcgtgagt
    49561 atgtgctgag cgatgaaacc agcgtggacg acattgcctt tacccgcaaa gggctgacct
    49621 cggaagtggt cgcggcggta gcgaagattt gctccaacgc ggacctgatc tacggcgcga
    49681 agaaaatgcc ggtaatcaaa aaggccaata ccaccatcgg tattccgggc accttcagcg
    49741 cccgtttgca gccaaatgac acccgtgacg acgtgcaaag tatcgctgca caaatctacg
    49801 aagggctttc cttcggggtg ggcgatgcgg tgatcggcgt taacccggta actgacgacg
    49861 tggaaaactt aagccgcgtg ttggacacca tttacggcgt gatcgacaaa ttcaacatcc
    49921 caactcaggg ctgcgtactg gcgcacgtca ccacccagat cgaagcgatt cgtcgcggcg
    49981 cgcctggcgg actgattttc cagagtatct gtggcagcga aaaagggctg aaagagtttg
    50041 gcgtggaact ggcgatgctc gacgaagcgc gcgcagtggg tgctgagttc aaccgtatcg
    50101 ccggggaaaa ctgcctctac ttcgaaaccg ggcaaggttc ggcgctgtct gctggcgcta
    50161 acttcggcgc agactaggtg acgatggaag cacgtaacta tgggctggcg cgtcattacg
    50221 atccgtttat cgtcaacacc gtggtcggct ttattgggcc ggagtatctc tacaacgacc
    50281 gccagattat ccgtgctggc ttagaagatc actttatggg caagctgagc ggcatctcta
    50341 tgggctgtga ctgctgttac accaaccacg ctgatgctga ccagaacctc aacgaaaacc
    50401 tgatgatcct gctcgccacc gcaggctgca actacatcat ggggatgccg ctgggcgatg
    50461 acatcatgct caactatcag accaccgcat tccacgacac tgccactgtg cgtcagttac
    50521 tcaacctgcg cccgtcaccg gagtttgaac gctggctgga aagcatgggc attatggcaa
    50581 acggtcgcct gaccaaacgg gcgggcgatc cgtcactgtt cttctgatga cgcggagata
    50641 acacatcatg gatcaaaaac agattgaaga aattgtacgc agcgtgatgg cgtcaatggg
    50701 acaagcggcc cccgcgccgt cagaagcaaa gtgcgccacc actaactgtg cggcaccggt
    50761 gacctcggaa agctgcgcgc tggatttagg ttctgcggaa gcaaaagcgt ggattggtgt
    50821 tgaaaacccg catcgcgcag acgtattaac agaactgcgc cgcagcaccg tggcccgcgt
    50881 ttgtaccggt cgtaccggtc cgcgtccacg tacgcaggcg ctgctgcgtt tcctggccga
    50941 tcactctcgt tcgaaagata ccgtactgaa agaagtgccg gaagagtggg tgaaagcgca
    51001 gggcttgctg gaagtacgct ccgaaattag cgacaaaaat ctctacctga ctcgcccgga
    51061 tatgggccgc cgcctgtgtg cagaagctgt tgaagcgctg aaagcacagt gtgttgctaa
    51121 tccggacgta caggtcgtta tttctgatgg cctgtcaact gatgccatca ccgtgaacta
    51181 cgaagagatc ctgccgccgc tgatggcggg cctgaaacag gccgggctga aagtcggcac
    51241 gccattcttt gttcgttatg gtcgcgtgaa gattgaagat cagatcggtg agatcctcgg
    51301 cgcgaaagtg gtgatcctgc tggtggggga acgtccgggg ttgggtcagt cagaaagcct
    51361 ctcctgttac gccgtttact cgccgcgtat ggcgaccacc gttgaggccg atcgcacctg
    51421 catctctaac attcaccagg gcggcacgcc gccggttgaa gccgcagccg tcattgtgga
    51481 tttggcgaaa cgtatgctgg agcagaaagc atccggcatc aacatgaccc gataaggagg
    51541 catcatgcca gctttagatt tgattcgacc gtcggtaacc gctatgcggg tgattgcctc
    51601 cgttaactct gaatttgcgc gtgaactaaa attgccgccg catattcgta gcctcgggct
    51661 gatttctgct gattccgatg acgtcaccta cattgcggct gacgaagcga ccaagcaggc
    51721 gatggtcgaa gtggtatatg gccgctcgct gtatgcggga gcagcacacg gaccgtcacc
    51781 gactgccggt gaagtgctga ttatgcttgg tgggccaaac ccggcggaag tgcgtgctgg
    51841 tctggatgcg atggttgcgc atattgaaaa tggcgctgct ttccagtggg ctaatgacgc
    51901 agaaaacacc gcattcctgg cacatgtagt ttcgcgtacc ggttcttatc tttcatcaac
    51961 cgccgggatc acgcttggcg atccgatggc gtatctggtg gcaccgccgc tggaagcgac
    52021 ctacggcatt gatgcagcgt tgaaatctgc cgacgtacag ctggtgacct atgtcccgcc
    52081 accgtctgaa accaactact cggcagcatt tttaaccggt agccaggccg cgtgtaaagc
    52141 agcctgtaac gcctttaccg atgcagtgct ggaaatcgcg cgtaatccaa tccagcgtgc
    52201 gtaacggagg ttgccgatga tcaatgcact gggattgctg gaagtggacg gcatggtcgc
    52261 cgcgatagat gcagcggatg ccatgctcaa agcagctaac gttcgtctgc tcagtcacga
    52321 agtgcttgac cctggtcgct taacgctggt ggtggaaggc gatctggcgg cgtgtcgtgc
    52381 ggcgctggac gcaggttgtg ctgccgcgat gcgtaccggg cgtgtcatca gccgcaagga
    52441 gatcggtcgg ccagacgatg acacccagtg gctggttact ggctttaacc gccagccgaa
    52501 gcaacccgta aaggaacccg acgcgccagt tatcgtcgcg gaatctgctg acgagttgtt
    52561 ggcgctgtta acatcagtac gtcagggaat gacggcagga gaagtggctg cccactttgg
    52621 ctggccgctg gaaaaagcca gaaatgcgct cgaacagctc ttttctgccg ggacgttacg
    52681 taaacgcagt agtcgttatc gtctcaagcc ccattaacct gtcggaggtg ccgggtgtcc
    52741 tacaacaccc ggcattaaca tcatgaaaaa gacccgtaca gccaatttgc accatcttta
    52801 tcatgaaccc ttacccgaaa acctgaagct cacgccgaag gtcgaagtgg ataatgttca
    52861 tcaacgacag acaacggatg tctatgaaca tgctttgacg attaccgcct ggcagcagat
    52921 ttacgatcag ctgcatccgg gcaagtttca tggtgaattt acggaaattc tgctcgatga
    52981 tattcaggtt tttcgtgaat acaccggtct ggcgctgcgt cagtcgtgcc tggtctggcc
    53041 gaactcgttc tggtttggca ttccggcgac gcgcggtgag cagggattta tcggttcgca
    53101 atgtctggga agcgcagaaa tagcgacgcg ccccggtgga accgagtttg aattaagtac
    53161 gccggacgat tatacgatcc tgggcgtagt gctgtctgaa gatgtcatca cccgtcaggc
    53221 taactttttg cacaatccgg atcgggtatt acatatgctg cgtagccagt cggcgctgga
    53281 agtgaaagag cagcataaag ccgcgctgtg gggctttgtc caacaggcgc tggcgacgtt
    53341 ttgcgagaac ccggaaaatc tccatcagcc tgcagtgcga aaagtgctgg gggataattt
    53401 gctaatggcg atgggggcga tgctggaaga cgcgcaacca atggtgacgg cggaaagcat
    53461 cagtcatcag agttaccgta gattactttc ccgcgcccgt gaatatgtgc tggaaaatat
    53521 gtctgagctg gtgacggtgc tggacttgtg taatcaactg catgtcagtc gccgcacgct
    53581 acaaaacgcg tttcacgcta ttttaggcat tggcccaaac gcgtggctga aacgcattcg
    53641 cctgaacgcc gtacgccgcg aactgataag cccgtggtcg caaagcacaa cggtaaaaga
    53701 cgccgccatg cagtggggat tctggcatct ggggcaattt gccacggatt accagcagct
    53761 gtttgccgag aagccgtcgt tgacgttgca tcagcggatg cgggaatggg ggtgagggag
    53821 ttacacccaa tccctgacct taataaactc acttaacgcc gcttctgggc tgccatcttt
    53881 tggctgataa tcatactccc agcgtaccag tggcggcatt gacatcagga tcgactcggt
    53941 gcgcccgcca gtttgcaggc caaacagagt gccgcgatcc cagaccagat tgaactcgac
    54001 ataacgaccg cgacggtaga gctgaaaatt gcgctcgcgc tcgccgtagg ccatcgcttt
    54061 acgtcgctct acaattggta aataagcgtc ggtgtagcct ttgcctaccg cctgcataaa
    54121 ggcaaaacag tggtcgaaat ctggcgtgtt cagatcatca aagaacagcc cgccaatacc
    54181 gcgctgttcg ttgcgatgtt tgaggtagaa gtaatcgtcg caccactttt tgtaacgggg
    54241 ataaacgtct tcaccaaatg gcaggcacag gtcacgggcg gtgcggtgcc agtgaatggc
    54301 gtcttcttca aaaccataga aaggggttaa atcgaagccg ccgccaaacc accagacggg
    54361 ctctgcaccc ggtttttcgg caataaaaaa acgcacattc gcgtggctgg tgggaacata
    54421 cgggttatgc ggatgcacta ccagtgaaac gcccatcgcc tcgaaactgc gcccggcaag
    54481 ttccgggcga tgagcggtgg cggaagcagg catcgcctcg ccgtggacgt gagaaaagtt
    54541 gacgcctgcc tgttcgaaaa caccaccatt acgcaacacc cgactacgcc cgccgccgcc
    54601 agcttcgcgc tgccaactat cttcgacaaa ttctgcgcca tcgacggcgg tcagctgctg
    54661 acaaatcgta tcctgaaggt tgagcagaaa ctgtttaacc tggtgtgcgt cgggtttcat
    54721 aacttatcgc tttttcgaat gtgctttctg gttgtcgaac cagtggaaat aactgatcac
    54781 gccttcagca atcgctgtgg cgattttctg acgaaacgcc accgtgccta acagccgctc
    54841 ttcttccggg ttggtgataa acgaggtttc caccagcacc gaaggaaccg acggcgattt
    54901 caacaccaca aacgcggctt gttcggtgtt gcggctgtgc agtttatgca ccggcttaat
    54961 ctttttcaga atatgcgagc caagcgtcag gctgttttta atagtatcgg tttgcaccag
    55021 atcaaacagc acttgctgca ataggtgatc cttgtcagtc gcctttttac cggcaacttc
    55081 atcggcgcgg ttttcgcgtt cagacaggta tttcgccatt gcgctacttg ccccacggtt
    55141 agagagggca aataccgaag caccggcagc tttcgggttg gtaaagccat cggcatgaat
    55201 tgacataaac agatctgcgc catgtttatg ggcgatttca acgcgatcgt aaagtgggat
    55261 aaacgtatcg ccagaacgcg ttaaacgcgc atcaatccca tgattacgca aaatggaacg
    55321 gacgttttta gcaatcgcca gcaccacatg tttttctttc gaaccgttgc gtccgatcgc
    55381 tccggtatca attccgccgt ggcctggatc gagaacaacg acacgtttgc cgccagattt
    55441 tttggctttc ggcttgctgt gtccgttgct ggtttttaaa ggttcgtctt tggcgatggc
    55501 ttgcgacatt cctgacaacg tcagggcagc caaaccggct ttcagcacct ggcggcgcga
    55561 agtgagtgtt tttagtggtt taaaagtgct catacggcct gagttgtaat aataaagttc
    55621 cagatgttat atcgtatcgc gtaatccgtt acgacggttt gatgttgaga attgttttac
    55681 ttttcatttc aatagttgac aactccccat tatgccactt tttcaaccga ttttcgtcag
    55741 atattggcta attcgaccgt tcacgccata atcagccttt taacccgcaa aaaggtaacg
    55801 aataccatgg agatacgcgt atttcgccag gaagatttcg aagaggtcat caccctttgg
    55861 gagcgttgcg acttgctgcg tccgtggaac gatccggaaa tggacatcga gcgtaagatg
    55921 aaccatgacg tcagtttgtt tttggtcgct gaggtaaacg gtgaagtggt cggaacggtg
    55981 atgggcggtt atgacgggca tcgcgggtct gcttattatc ttggcgtgca tccagagttt
    56041 cgtgggcgtg ggattgccaa tgcgttgctt aatcggctgg agaaaaagct gattgctcgt
    56101 ggctgcccga aaattcagat caatgtgccg gaagataacg acatggtgct cggaatgtat
    56161 gaacgcctgg gatatgaaca cgccgacgtg ctgagtctgg gtaagcgttt gattgaagat
    56221 gaagagtact gagttccatc ctgtccatta tgatgcgcac ggacgcctgc gtttaccctt
    56281 gctcttctgg cttgtgctat tgcttcaggc gcgaacctgg gtgctgtttg tcattgccgg
    56341 tgcgtcgcgc gagcagggta ctgcgctgct aaatctgttt tatcccgatc acgataattt
    56401 ctggctgggg ctgattccag gcattccggc ggtgctggcg tttttgttga gcggtcggcg
    56461 ggctacattt cctcgcactt ggcatgtgct ctattttctg ttgctgttgg cgcaggtggt
    56521 tttactttgc tggcaaccgt ggctgtggct gaacggtgaa tccgttagcg gtatcggtct
    56581 ggcgctggta gtggcggata ttgtggcgtt aatctggctg ctgaccaatc gacgtttacg
    56641 cgcttgtttt aatgaagaaa aagaataacg gcactttttg gtgaatttgc actccaagca
    56701 acgttattga ataaccaaag gaagtgacat gaaatcgctg cgtttaatgt tatgcgctat
    56761 gccgttgatg ctgaccggct gttccacgat gtcgtcagtt aactggtcgg ccgctaaccc
    56821 gtggaactgg tttggatcgt ccaccaaagt gagcgagcag ggcgtgggtg aattaacggc
    56881 gtccacacca ctgcaagaac aagccattgc tgatgcgctt gatggcgatt atcgcctgcg
    56941 cagcggaatg aaaaccgcga acggcaacgt ggtgcgcttt tttgaagtga tgaaaggcga
    57001 caacgtggcg atggtgatta acggcgatca aggcacgatc agtcgcattg acgtgctgga
    57061 tagcgatatt cctgctgaca ccggtgttaa aatcggtaca ccgtttagcg acctttacag
    57121 caaagcattt ggcaattgcc aaaaagctga tggtgatgat aatcgtgccg tcgaatgtaa
    57181 agccgaaggc agtcaacata ttagctacca gttcagcggg gaatggcgtg gtcctgaagg
    57241 gttaatgcct tcggacgata ccctgaaaaa ctggaaagtc agtaaaatta tctggcggcg
    57301 ttaatttgcg tctgaacaaa ccgccgccgc gaaaaaacac gtaaaatagc gctcaacaat
    57361 gccacggatt gcgtggcatt cttattttca ggaggaacaa tgtctcaggt tcagagtggc
    57421 attttgccag aacattgccg cgcggcgatt tggatcgaag ccaacgtgaa aggggaagtt
    57481 gacgccctgc gtgcggccag taaaacattt gccgacaaac tggcaacttt tgaagcgaaa
    57541 ttcccggacg cgcatcttgg tgcggtggtt gcctttggta acaacacctg gcgcgctctg
    57601 agcggcggcg ttggggcaga agagctgaaa gattttccgg gctacggtaa aggccttgcg
    57661 ccgaccaccc agttcgatgt gttgatccac attctttctc tgcgtcacga cgtaaacttc
    57721 tctgtcgccc aggcggcgat ggaagccttt ggtgactgca ttgaagtgaa agaagagatc
    57781 cacggcttcc gttgggtgga agagcgtgac ctgagcggct ttgttgacgg tacagaaaac
    57841 ccggcgggtg aagagacgcg tcgcgaagtg gcggttatca aagacggcgt ggatgccggc
    57901 ggcagctatg tgtttgtgca gcgttgggag cacaacctga agcagctcaa ccggatgagc
    57961 gttcacgatc aggagatgat gatcgggcgc accaaagagg ccaacgaaga gattgacggc
    58021 gacgaacgtc cggaaacctc tcacctcacc cgcgttgatc tgaaagaaga tggcaaaggg
    58081 ctgaagattg ttcgccagag cctgccgtac ggcaccgcca gtggcactca cggtctgtac
    58141 ttctgcgcct actgcgcgcg tctgcataac attgagcagc aactgctgag catgtttggc
    58201 gataccgatg gtaagcgtga tgcgatgttg cgtttcacca aaccggtaac cggcggctat
    58261 tacttcgcgc cgtcgctgga caagttaatg gcgttgtaag cgtttctgat tcgatgatta
    58321 aaggccagcc agtaacaaaa ctgactggcc tttttattac ttttgcttca acgccgcata
    58381 cacctgatca actacccaac cataagttgc aatcggtaac tgaccgcttt cgaacatatt
    58441 gggatttttt tgtggatcgg caactggcga atggggcttg ttgcttaaca tcacaatcgc
    58501 catatgattg accggatcga taacggttac tgtcccggtc cagccagtgt gaccgtaggt
    58561 ttgcgggctt gccagcgtgc caaacgtcgg cgtcatggtg gcattaccat tcacgcgcca
    58621 gccgaggcca aaagtggcat cttccttaga gctggtggtg aacatcttca ccgtttccgc
    58681 actgaacagc tgcacatcgc catagccccc gccgttcagc atcgtttgca ttaacaccgc
    58741 aatatcgccg gtattggaaa acaaacctgc gtgcccggaa acgccgccca tcgaataaaa
    58801 ggctttttca tcgtgcacct gaccccagag agtggaggtg cggatattcg ggaaatggat
    58861 cacgccatcg cgggtattgc cgttcaattc cgtggcggca atttgttgcg gtttaaagcc
    58921 tttcagcagc gggttaaata ccgtatgtgt caggccgagc gggcgataaa tcgactcttc
    58981 aacatagcgg tcaagcggct gaccggtaac cgactcgacg ataaatccaa gcagcatata
    59041 atcgacatcg ctgtagatat gtttgctgcc gggctgatat tccagcggcg tgcgcttgat
    59101 catctccagc gtctggcctt tatcctggga atataacgcg cccgcgaccg ctttattcgg
    59161 gtattgcgga tctgccggga aaccgccgct gtgatgcagc aggtcagaaa tccgcagggt
    59221 gtttttgcct ttaatggtgt catttggact atcggcaaac cccggaatat atttcgcaat
    59281 ccgatcgtca ggatgcagtt tgccttcgct catcagcttt tgcagggcga agttcgtggc
    59341 gtacattttg gtgtttgagg ccaggtcata cagcgtcccg gtggtggctt tgacaggctg
    59401 ttccatcaac acgctgccat cgtacttttt cgccgctccc caggctttgc gatacacaat
    59461 ctgattatct ttaatgatca gcaggtttac gccgggataa ccgacatcaa tttgctggct
    59521 aatccagcga tccatctggt taagccgttc gacgttaaac cctgctttct ctggcgagct
    59581 ttctgtcaga acagggtatt ttgcggcgct gacgctacaa ctaacagcca gcagagaaag
    59641 atagagcatt gtccgtttca tgatatcagt ccagattgac gttacggcag ccaaagagcg
    59701 tggtgaaaat aaacccgcaa acccatgcca ccagaatccc gccagcataa accgccatgg
    59761 ccggaaggat gccttgtgcg gaagtcatca gcggcagcgc taccagacca gacggcccaa
    59821 aggcgctgtt taagcccatc ggcagacccc atcaggctat caggccgata aacaaaccgc
    59881 ccgccgcgcc gcctaaacag gcggtaacaa acggcttcat gcggggcagg gtgacaccgt
    59941 aaatcagcgg ttcaccaacg cccagcaggc cgggaataat cgccccgcgt acctgactgc
    60001 gtagcgcact gtgcggttgc gcccgccagt agagtgccag cgccgcgccc acctggcccg
    60061 cgcctgccat tgaaaggatg ggaaataagc tgttgaatcc ctagctgtcc attaacgcga
    60121 ggtaaacagg aataaagccc tgatgcacgc caaataccac ggcgatcagg aacagcccgg
    60181 ctaaaaccgc acaaccgaac ggattactgt tcaggtgcat aaacagccat gacatacctt
    60241 cgaataacca gccacccagc ggcataatga tcaggtacgc aagcgtagcg gtgatcagca
    60301 gggtgattaa cgaggtcagc agcatgtcga gatcgtccga cataaagcgg cgcaccatgc
    60361 cttcaatgcg ggcgcaggcc cacgcggcaa tcagcacgcc gataatattg ccgcgcggat
    60421 cgatgggcag accaaagaaa tcgtgaaaac cggcgtagtc accggtggtt gcagcggggt
    60481 tataaccgag caaaaagagt gcggcaataa ttgcgccatt gacgcccgtg ccgccaaatg
    60541 cctgggcggc gttatagccc accagaatca ccaggaaagt gaacaaacct ttgctgaaca
    60601 ccttcataaa attcagcgcg tcgggtagcg tgccctgagc atctgccgga acgtgcatca
    60661 ccgtggcaat taacgtcgct atccccagca gcagaccggc ggcaataaaa ccggggatca
    60721 gcggcgtgaa gatggtggcg aatttggcga gaaattgttg cacgccggag gtttgtttgg
    60781 cttttaactg acgtttgttc tgggcggcga tttctgcggc atcctgtacc ggtgcctcgc
    60841 ccagcagctc gctcatcgct tttgcggcac gatgcgcttt accaggccca aaaacgacct
    60901 gcacctggtc gctggtcaaa atgacgccct tcacgccttc cagcgttttg atattgggat
    60961 caaccagtga actgtcatgt acacccagac gcaggtgcgt catacagtta ccacaactgg
    61021 cgatatttcc cagtccgccg acacgggtaa gaatggtgtt cagaagttca ctgctgatct
    61081 ctttggccat gggttattcc ttgtctaaaa cctgacgaat aaagccgccg tgttgatcca
    61141 ggcgtttttt agcttctgcg gcatcgagat ttttcagcac catcacaatg gccgttttac
    61201 agttgcgctc gcaggcaatt aacgccgctt ccgcttgttc ggcattacat ccggtggcgt
    61261 ttttaacgat attgacctgg cgcacatgca gtttttcgtt ggtggcgacc acatcgacca
    61321 tcaggttgcc gaacactttg ccggatttaa tcatcagccc ggtggaaagc atattgagca
    61381 ccagtttctg cgctgtgcct gctttcattc gcgaagaacc agtgaccact tcggcaccta
    61441 caatcggtgt aatggcaaac tcggcggtgg tggaaacggc actccccgga ttacaggaaa
    61501 ttcccactgt gcggcaaccg agctggcggg cgtattccag tccggcaatc acatacggcg
    61561 tacgaccgct ggcggcaata ccgaccacca catcctgtgc cgttaaattt atatttttca
    61621 gatcgttaat gccgccttct cggctatctt ccgtgccttc caccgcgtgc tgaatggcat
    61681 attcgccgcc agcaatcaaa ccaaccacca gacccggttt cacgccgtag gtgggcggac
    61741 attcgctggc atccagaatc cccagacgac cggatgttcc cgcaccgaga taaatcagac
    61801 gaccgccgcc gcttacctgg gcgtggataa catcgatcgc cgcggcgata tccggcagga
    61861 cgcgctcaac ggcaagcggt acggttttat cttcatcgtt gataatccgg cacatttcca
    61921 gcgtcgatac gcggtcaatt tcagccgagg cggtgttcga gccttcagta atcatctttt
    61981 caagttgcat cggtggtgac cttactattt acgatcaaat aattgcagat gtatggaatc
    62041 atatatcccg aattgacaga cttaaaggaa tcattgagtc tacacttgca gagtgtgaag
    62101 agcgtcataa gaacttaagc ggaatttttg ctgccagccc gggagagtgg aagatgttgt
    62161 acctgacgaa aatacgcaat gcggaaagtg aatttaccga gaacgagcaa aaaattgctg
    62221 actttctgcg ggccaacgtg agtgagctga aatccgtctc ttcgcgcaag atggcgaaac
    62281 agctcggcat cagtcagtcg agcattgtga aatttgccca gaagctcggc gcgcaaggat
    62341 ttactgaatt gcgtatggcg ttaattggcg aatacagtgc cagccgggag aaaacgaatg
    62401 ccacggcgct gcacctgcac agttcgatta ccagtgatga ttcgctggaa gtgattgccc
    62461 gcaaactgaa tcgcgaaaaa gagctggcgc tggagcaaac ctgtgcgttg ttcgactacg
    62521 ctcggctgca aaaaatcatt gaggtcatca gtaaagcacc gtttatccag ataacgggcc
    62581 tgggcgggtc ggcgctggtg gggcgcgatc tgtcgttcaa actgatgaaa attggctatc
    62641 gcgtggccta tgaagccgac acacacgtac aagccaccgt ttcccaggcg ttgaaaaaag
    62701 gcgatgtaca aatcgctatc tcttacagcg gcagtaaaaa agagattgtc ttgtgcgcag
    62761 aagcggcacg aaagcagggg gcaacggtga ttgccattac ttctctggct gactctccgc
    62821 tgcggcggct ggcgcatttc acgctcgata ccgtttccgg tgaaaccgaa tggcgtagct
    62881 cgtcgatgtc tacccgcacg gcgcaaaatt ctgttaccga tttactgttt gttggtcttg
    62941 tgcaactcaa tgatgtggag tcattgaaaa tgattcagcg cagcagtgag ctaacacaac
    63001 gcctgaaatg atgcataaag cagcgactgg attgagattt tcctgaatta gtgagctgat
    63061 ccgcagcaat attttgttta tcctgtattt tcagagggaa tggagtgtaa cgctctgtat
    63121 taacaaggag agcattaaaa tgggtaaact cacgggcaag acagcactga ttacgggcgc
    63181 attgcaggga attggcgaag gaattgccag aacttttgcg cgtcatggcg caaacctaat
    63241 cttgctggat atctcccctg agatcgaaaa gctggcggac gaactgtgtg gtcgtggtca
    63301 tcgctgtacg gcggttgtcg ccgatgtgcg tgacccggcg tcggtagccg cagctatcaa
    63361 acgcgcgaag gaaaaagaag ggcgcattga tatcctggtg aataacgcag gcgtttgtcg
    63421 tctgggcagt ttcctcgata tgagcgatga cgatcgcgat ttccatattg acatcaatat
    63481 taaaggcgta tggaacgtca cgaaggcggt gctgccggag atgattgccc gcaaagatgg
    63541 tcgcattgtg atgatgtctt cagtcactgg tgatatggtg gccgatcctg gcgaaacggc
    63601 gtacgcctta acgaaagcgg cgattgttgg cctgacaaaa tcgctggcgg tggagtacgc
    63661 gcagtctggt attcgcgtta acgccatttg cccgggatac gtgcgcacac caatggcgga
    63721 aagcattgcc cgccagtcga acccggaaga tccagagtcg gtgctgactg aaatggcgaa
    63781 agcaatcccg ctgcgtcgcc tcgccgatcc gctggaagtc ggcgaactgg cggccttcct
    63841 cgcatcggat gaatccagct atttaaccgg tacacagaat gtgattgatg gcggcagcac
    63901 actgccggag acggttagcg tcggtatctg attcacctct gtttcctccc tgcatttgtg
    63961 gggaggattt cgttttaaac ccggttctta ttcccttttc aacttccaaa tcaccaaacg
    64021 gtatataaaa ccgttactcc tttcacgtcc gttataaata tgatggctat tagaaagtca
    64081 ttaaatttat aagggtgcgc aatggccgtt aacttactga aaaagaactc actcgcgctg
    64141 gtcgcttctc tgctgctggc gggccatgta caggcaacgg aactgctgaa cagttcttat
    64201 gacgtctccc gcgagctgtt tgccgccctg aacccgccgt ttgagcaaca atggaccaaa
    64261 gaaaacggcg gcgacaaact gacaataaaa caatctcatg ccgggtcatc aaaacaggcg
    64321 ctggcaattt tgcagggctt aaaagcagac gttgtcactt ataaccaggt gactgacgta
    64381 caaatcctgc atgacaaagg caagctgatc ccggcggact ggcaaacgcg cctgccaaat
    64441 aatagctcgc cattctactc caccatgggc ttcctggtgc gtaagggcaa cccgaagaat
    64501 atccacgact ggaacgacct ggtgcgctcc gatgtgaagc tgattttccc gaacccgaaa
    64561 acgtcgggta acgcgcgtta cacctatctg gcggcatggg gcgcagcgga taaagctgac
    64621 ggtggcgata aagccaaaac cgaacagttt atgacccagt tcctgaaaaa cgttgaagtg
    64681 ttcgatactg gcggtcgtgg cgcgaccacc acttttgccg agcgcggcct gggcgatgtg
    64741 ctgatcagct tcgagtcgga agtgaacaac atccgtaaac agtatgaagc gcagggtttt
    64801 gaagtggtga ttccgaaaac caacattctg gcggaattcc cggtggcgtg ggttgataaa
    64861 aacgtgcagg ccaacggtac ggaaaaagcc gccaaagcct accttaactg gctctacagc
    64921 ccgcaggcgc aaaccatcat caccgactat tactaccgcg tgaataaccc ggaagtcatg
    64981 gataaactga aagacaaatt cccgcagacc gagctgttcc gcgtggaaga caaatttggc
    65041 tcctggccgg aagtgatgaa aacccacttc accagcggcg gcgagttaga caagctgtta
    65101 gcggcggggc gtaattaatg tttgcagttt catcaagacg cgtgctgccg ggctttacct
    65161 taagcctcgg caccagtctg ctgtttgtgt gcctgatttt gctgctgccg ctctccgcgc
    65221 tggtgatgca gctggctcag atgagctggg cgcagtactg ggaggtgatc accaacccgc
    65281 aggtagtagc ggcctacaaa gtaacgctgc tgtcagcgtt tgtggcatcg atttttaacg
    65341 gcgtttttgg cctgctgatg gcgtggatct taacccgcta tcgctttccg ggccgcacgc
    65401 tgcttgatgc gctgatggat ttaccctttg cgctgccaac ggcagtcgcc ggtttaacgc
    65461 tagcctcgct cttttctgta aacggttttt acggtgaatg gctggcgaag tttgatatca
    65521 aagtcaccta tacctggctg gggattgcgg tggcgatggc ctttaccagc attccgtttg
    65581 tggtacgtac cgtgcagccg gtgctggaag agttaggccc ggaatatgaa gaagcggcag
    65641 aaacgcttgg cgcaacgcgc tggcagagtt tctgcaaagt ggtgctgccg gaactttctc
    65701 cggcgctggt ggcgggcgtg gcgctgtcgt ttacccgtag tcttggtgaa tttggcgcgg
    65761 tgatttttat cgccggaaat atcgcgtgga agacggaagt gacgtcgctg atgatttttg
    65821 tgcgcttaca ggagtttgat tacccggcag cgagcgcgat tgcttcggtg atcctcgcgg
    65881 catctctgct gctgctgttc tcaattaaca ctctgcaaag tcgctttggt cggcgtgtgg
    65941 taggtcatta atggcggaag ttacccaatt gaagcgttat gacgcgcgcc cgattaactg
    66001 gggcaaatgg tttctgattg gcatcgggat gctggtatcg gcgttcatcc tgctggtgcc
    66061 gatgatttat atcttcgtac aggcgttcag caaagggctg atgccggttt tacagaatct
    66121 ggccgatccg gacatgctgc acgccatctg gctgacggtg atgatcgcgc tgattgccgt
    66181 accggtaaac ctggtgttcg gcattctgct ggcctggctg gtgacgcgct ttaacttccc
    66241 tggacgccag ttactgctga cgctactgga cattccgttt gccgtatcgc cggtggttgc
    66301 cggtctggtg tatctgctgt tctacggctc taacggcccg ctgggcggct ggctggatga
    66361 acataatctg caaattatgt tctcctggcc cggaatggtg ctggtcacca tctttgtgac
    66421 gtgtccgttt gtggtgcgcg agctggtgcc ggtgatgtta agccagggca gccaggaaga
    66481 cgaagcggcg attttgcttg gcgcgtctgg ctggcagatg tttcgccgcg tcacattgcc
    66541 gaacattcgc tgggcgctgc tttatggcgt ggtgttgacc aacgcccgcg caattggcga
    66601 gtttggcgcg gtgtcggtgg tttccggctc gattcgcggc gaaaccctgt cgctgccgtt
    66661 acagattgaa ttgctggagc aggactacaa caccgtcggc tcctttaccg ctgcggcact
    66721 gttaacgctg atggcgatta tcaccctgtt tttaaaaagt atgttgcagt ggcgcctgga
    66781 gaatcaggaa aaacgcgcac agcaggagga acatcatgag cattgagatt gccaatatta
    66841 agaagtcgtt tggtcgcacc caggtgctga acgatatctc actggatatt ccttcaggtc
    66901 agatggtcgc gttgctgggg ccgtccggtt ccgggaaaac cacgctgctg cgcattatcg
    66961 ccgggctgga gcatcaaacc agcgggcata ttcgcttcca cggcaccgac gtcagccgcc
    67021 tgcacgcgcg cgatcgcaag gtcggtttcg tgttccagca ttacgcgctg ttccgccata
    67081 tgacggtatt cgacaatatc gcttttggcc tgacggtgct gccgcgtcgc gatcgcccga
    67141 atgccgcagc catcaaagcg aaagtgacaa aattgctgga gatggtgcag cttgctcatc
    67201 tggcggatcg ttatccggca cagctttccg gcggccagaa acagcgcgtg gcgctggcgc
    67261 gcgcgctggc tgtagaaccg caaattctgc tgcttgatga accgtttggc gcgctggatg
    67321 cgcaggtgcg taaagagctg cgtcgctggc tgcgtcaact gcatgaagag ctgaaattca
    67381 ccagcgtgtt cgtgacccac gaccaggaag aagcgacgga agtagctgat cgtgtagtgg
    67441 tgatgagcca gggcaatatt gaacaggctg atgcgccgga gcaggtatgg cgcgaaccag
    67501 cgacccgttt tgtgctcgaa tttatgggcg aagtgaaccg cctgcaggga accattcgcg
    67561 gcgggcagtt ccatgttggc gcacatcgct ggccgctggg ctacacacct gcttatcagg
    67621 ggccggtgga tctcttcctg cgcccgtggg aagtggatat cagccgccgt accagtctcg
    67681 attcgccgct gccggtacag gtactggaag ccagcccgaa aggtcactac acccaattag
    67741 tggtgcagcc gctggggtgg tacaacgaac cgctgacggt cgtgatgcat ggcgacgatg
    67801 ccccgcagcg tggcgagcgt ttattcgttg gtctgcaaca tgcgcggctg tataacggcg
    67861 acgagcgtat cgaaacccgc gatgaggaac ttgctctcgc acaaagcgcc tgataggttg
    67921 agtgaatgtt aaacgcccgg aggcgcttcc cgcgatccgg gctttttaat ggcaaggttt
    67981 gtaacctgta gacctgataa gacgcgcaag cgtcgcatca ggcaacacca cgtatggata
    68041 gagatcgtga gtacattaga acaaacaata ggcaatacgc ctctggtgaa gttgcagcga
    68101 atggggccgg ataacggcag tgaagtgtgg ttaaaactgg aaggcaataa cccggcaggt
    68161 tcggtgaaag atcgtgcggc actttcgatg atcgtcgagg cggaaaagcg cggggaaatt
    68221 aaaccgggtg atgtcttaat cgaagccacc agtggtaaca ccggcattgc gctggcaatg
    68281 attgccgcgc tgaaaggcta tcgcatgaaa ttgctgatgc ccgacaacat gagccaggaa
    68341 cgccgtgcgg cgatgcgtgc ttatggtgcg gaactgattc ttgtcaccaa agagcagggc
    68401 atggaaggtg cgcgcgatct ggcgctggag atggcgaatc gtggcgaagg aaagctgctc
    68461 gatcagttca ataatcccga taacccttac gcccattaca ccactaccgg gccggaaatc
    68521 tggcagcaaa ccggcgggcg cattacccat tttgtctcca gcatgggaac aaccggcact
    68581 atcaccggcg tttcacgctt tatgcgcgaa caatccaaac cagtaacgat tgttggcctg
    68641 cagccggaag aaggcagcag tattcccggc attcgccgct ggcctgcgga atatctgccg
    68701 gggattttca acgcttctct ggtggatgag gtgctggata ttcatcagcg cgatgcagaa
    68761 aacaccatgc gcgaactggc agtgcgggaa ggaatattct gtggcgtcag ctccggcggc
    68821 gcggttgccg gagcactgcg ggtggcaaaa gctaaccctg gtgcggtggt ggtagcgatc
    68881 atctgcgatc gtggcgatcg ctacctttcc accggggtgt ttggggaaga gcattttagt
    68941 cagggggcgg ggatttaaga ttattgcgca ggcaccgttt tatccgtctg cgcaattaag
    69001 gcatgtaaaa aaggctgtgc gttctcatcg ctcttaccgg ggatcttcac aatatacggc
    69061 ttcccggtaa tcgatgacga cgaagcaacc ttatcaataa actgctcggc agtgtcaatg
    69121 cggttacggg tattgccgag cttcagacgc agatgagaaa ccgcttcatc gcaggtatgt
    69181 tcatcaccgt tgcgcacaaa tatcaaatcc tttttctgtg ctaatccctc cagcatggcg
    69241 ttgatgcggg cttcttcatg aacagttaac ttcgcgtaag cggggagtgt cattagtagt
    69301 gtaatgacca gacaaatgat tttcttcatt gaattacctt atccttgttt tgtgatagcc
    69361 gatttgactg ccagaatcag gtaaagtttc ggttggaata cgatgaggat catcggcaag
    69421 gagaaaaaat taaatgagta gtttgttgtt gtttaacgat aagagtaggg cgctgcaggc
    69481 ggatatcgtc gccgtgcagt cgcaggtagt gtacggcagc gtaggcaaca gcattgcggt
    69541 gcctgctata aaacagaacg gcttgaatgt ctttgccgtg ccgacggtat tgctgagcaa
    69601 tacgccgcat tatgacactt tctacggtgg tgcgattccg gacgaatggt ttagcggcta
    69661 tttgcgtgcg cttcaggagc gtgatgcgct gcgccaactt cgtgctgtaa ccacgggcta
    69721 tatgggaacg gcatcgcaaa tcaaaatcct tgccgagtgg ctgactgcgc tacgcaaaga
    69781 ccatcctgac ctattgatca tggtcgatcc ggtgattggc gatattgata gcggaattta
    69841 tgtcaaacct gaccttcccg aggcgtatcg acaatattta ctgccgctgg cgcaaggaat
    69901 tacccccaat atctttgagc tggaaatcct gaccggtaaa aattgccgcg atctcgacag
    69961 tgccattgct gccgcaaaaa gtctgctttc agacacatta aaatgggtgg tgattaccag
    70021 cgcctccggt aatgaagaaa atcaggagat gcaggtggtg gtggtcagtg ccgacagcgt
    70081 gaatgtcatt tcccattcac gggtaaaaac cgacctgaaa gggactggcg acctgttttg
    70141 tgctcagctt atcagtggtt tgctgaaagg gaaggcgtta aacgatgcgg tgcaccgtgc
    70201 ggggttgcgc gtactggaag tgatgcgcta tacccagcag catgagagcg atgaattgat
    70261 tttgccgccg ctggcggaag cataaaaaat ggcgccgatg g
//
LOCUS       DAETQN010000006        64812 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.006, whole genome shotgun sequence.
ACCESSION   DAETQN010000006 DAETQN010000000
VERSION     DAETQN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64812
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.006"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(36..1334)
                     /gene="eno"
                     /locus_tag="K0W68_000430"
     CDS             complement(36..1334)
                     /gene="eno"
                     /locus_tag="K0W68_000430"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBI3072103.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="K0W68_000431"
     CDS             complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="K0W68_000431"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBI3072104.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="K0W68_000432"
     CDS             complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="K0W68_000432"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBI3072105.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="K0W68_000433"
     CDS             complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="K0W68_000433"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HBI3072106.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="K0W68_000434"
     CDS             complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="K0W68_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HBI3072107.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4810..7044)
                     /locus_tag="K0W68_000435"
     CDS             complement(4810..7044)
                     /locus_tag="K0W68_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBI3072108.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="K0W68_000436"
     CDS             complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="K0W68_000436"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBI3072109.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLCLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8450..11206
                     /gene="barA"
                     /locus_tag="K0W68_000437"
     CDS             8450..11206
                     /gene="barA"
                     /locus_tag="K0W68_000437"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBI3072110.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="K0W68_000438"
     CDS             complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="K0W68_000438"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBI3072111.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12799..14139)
                     /locus_tag="K0W68_000439"
     CDS             complement(12799..14139)
                     /locus_tag="K0W68_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBI3072112.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="K0W68_000440"
     CDS             complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="K0W68_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBI3072113.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15928..16377)
                     /locus_tag="K0W68_000441"
     CDS             complement(15928..16377)
                     /locus_tag="K0W68_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBI3072114.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16395..17177)
                     /gene="truC"
                     /locus_tag="K0W68_000442"
     CDS             complement(16395..17177)
                     /gene="truC"
                     /locus_tag="K0W68_000442"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBI3072115.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17177..17506)
                     /locus_tag="K0W68_000443"
     CDS             complement(17177..17506)
                     /locus_tag="K0W68_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBI3072116.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18128..18673)
                     /gene="syd"
                     /locus_tag="K0W68_000444"
     CDS             complement(18128..18673)
                     /gene="syd"
                     /locus_tag="K0W68_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBI3072117.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18741..19589
                     /gene="queF"
                     /locus_tag="K0W68_000445"
     CDS             18741..19589
                     /gene="queF"
                     /locus_tag="K0W68_000445"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBI3072118.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19701..21065
                     /gene="ppnN"
                     /locus_tag="K0W68_000446"
                     /gene_synonym="ygdH"
     CDS             19701..21065
                     /gene="ppnN"
                     /locus_tag="K0W68_000446"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBI3072119.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21621..22910
                     /gene="sdaC"
                     /locus_tag="K0W68_000447"
     CDS             21621..22910
                     /gene="sdaC"
                     /locus_tag="K0W68_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBI3072120.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22968..24335
                     /gene="sdaB"
                     /locus_tag="K0W68_000448"
     CDS             22968..24335
                     /gene="sdaB"
                     /locus_tag="K0W68_000448"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBI3072121.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNASRMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24447..25202
                     /gene="xni"
                     /locus_tag="K0W68_000449"
     CDS             24447..25202
                     /gene="xni"
                     /locus_tag="K0W68_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBI3072122.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25307..26455)
                     /gene="fucO"
                     /locus_tag="K0W68_000450"
     CDS             complement(25307..26455)
                     /gene="fucO"
                     /locus_tag="K0W68_000450"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBI3072123.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26483..27130)
                     /gene="fucA"
                     /locus_tag="K0W68_000451"
     CDS             complement(26483..27130)
                     /gene="fucA"
                     /locus_tag="K0W68_000451"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBI3072124.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27677..28993
                     /gene="fucP"
                     /locus_tag="K0W68_000452"
     CDS             27677..28993
                     /gene="fucP"
                     /locus_tag="K0W68_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBI3072125.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29026..30801
                     /gene="fucI"
                     /locus_tag="K0W68_000453"
                     /pseudo
     CDS             29026..30801
                     /gene="fucI"
                     /locus_tag="K0W68_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            30910..32328
                     /gene="fucK"
                     /locus_tag="K0W68_000454"
     CDS             30910..32328
                     /gene="fucK"
                     /locus_tag="K0W68_000454"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HBI3072126.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32330..32752
                     /gene="fucU"
                     /locus_tag="K0W68_000455"
     CDS             32330..32752
                     /gene="fucU"
                     /locus_tag="K0W68_000455"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBI3072127.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32810..33541
                     /gene="fucR"
                     /locus_tag="K0W68_000456"
     CDS             32810..33541
                     /gene="fucR"
                     /locus_tag="K0W68_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBI3072128.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33585..34685)
                     /gene="rlmM"
                     /locus_tag="K0W68_000457"
     CDS             complement(33585..34685)
                     /gene="rlmM"
                     /locus_tag="K0W68_000457"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBI3072129.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34678..35073)
                     /locus_tag="K0W68_000458"
     CDS             complement(34678..35073)
                     /locus_tag="K0W68_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBI3072130.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35092..36009)
                     /gene="gcvA"
                     /locus_tag="K0W68_000459"
     CDS             complement(35092..36009)
                     /gene="gcvA"
                     /locus_tag="K0W68_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBI3072131.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36360..36587)
                     /locus_tag="K0W68_000460"
     CDS             complement(36360..36587)
                     /locus_tag="K0W68_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBI3072132.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36779..37984
                     /gene="csdA"
                     /locus_tag="K0W68_000461"
     CDS             36779..37984
                     /gene="csdA"
                     /locus_tag="K0W68_000461"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBI3072133.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37984..38427
                     /gene="csdE"
                     /locus_tag="K0W68_000462"
     CDS             37984..38427
                     /gene="csdE"
                     /locus_tag="K0W68_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBI3072134.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38478..39284)
                     /gene="tcdA"
                     /locus_tag="K0W68_000463"
     CDS             complement(38478..39284)
                     /gene="tcdA"
                     /locus_tag="K0W68_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBI3072135.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39361..40458)
                     /gene="mltA"
                     /locus_tag="K0W68_000464"
     CDS             complement(39361..40458)
                     /gene="mltA"
                     /locus_tag="K0W68_000464"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBI3072136.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40667..40743
                     /locus_tag="K0W68_000465"
     tRNA            40667..40743
                     /locus_tag="K0W68_000465"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40701..40703,aa:Met,seq:cat)
     gene            40777..40853
                     /locus_tag="K0W68_000466"
     tRNA            40777..40853
                     /locus_tag="K0W68_000466"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40811..40813,aa:Met,seq:cat)
     gene            40887..40963
                     /locus_tag="K0W68_000467"
     tRNA            40887..40963
                     /locus_tag="K0W68_000467"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40921..40923,aa:Met,seq:cat)
     gene            complement(41038..42291)
                     /gene="amiC"
                     /locus_tag="K0W68_000468"
     CDS             complement(41038..42291)
                     /gene="amiC"
                     /locus_tag="K0W68_000468"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBI3072137.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42523..43854
                     /gene="argA"
                     /locus_tag="K0W68_000469"
     CDS             42523..43854
                     /gene="argA"
                     /locus_tag="K0W68_000469"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBI3072138.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43916..45742)
                     /gene="recD"
                     /locus_tag="K0W68_000470"
     CDS             complement(43916..45742)
                     /gene="recD"
                     /locus_tag="K0W68_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBI3072139.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKRRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45742..49284)
                     /gene="recB"
                     /locus_tag="K0W68_000471"
     CDS             complement(45742..49284)
                     /gene="recB"
                     /locus_tag="K0W68_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBI3072140.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49277..52165)
                     /gene="ptrA"
                     /locus_tag="K0W68_000472"
     CDS             complement(49277..52165)
                     /gene="ptrA"
                     /locus_tag="K0W68_000472"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBI3072141.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52341..55709)
                     /gene="recC"
                     /locus_tag="K0W68_000473"
     CDS             complement(52341..55709)
                     /gene="recC"
                     /locus_tag="K0W68_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBI3072142.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55722..56045)
                     /locus_tag="K0W68_000474"
     CDS             complement(55722..56045)
                     /locus_tag="K0W68_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBI3072143.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(56030..56437)
                     /locus_tag="K0W68_000475"
     CDS             complement(56030..56437)
                     /locus_tag="K0W68_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBI3072144.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56434..56997)
                     /locus_tag="K0W68_000476"
     CDS             complement(56434..56997)
                     /locus_tag="K0W68_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBI3072145.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56988..57458)
                     /locus_tag="K0W68_000477"
     CDS             complement(56988..57458)
                     /locus_tag="K0W68_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBI3072146.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(57643..58437)
                     /gene="thyA"
                     /locus_tag="K0W68_000478"
     CDS             complement(57643..58437)
                     /gene="thyA"
                     /locus_tag="K0W68_000478"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBI3072147.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58444..59319)
                     /gene="lgt"
                     /locus_tag="K0W68_000479"
     CDS             complement(58444..59319)
                     /gene="lgt"
                     /locus_tag="K0W68_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBI3072148.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59470..61716)
                     /gene="ptsP"
                     /locus_tag="K0W68_000480"
     CDS             complement(59470..61716)
                     /gene="ptsP"
                     /locus_tag="K0W68_000480"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBI3072149.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61729..62259)
                     /gene="rppH"
                     /locus_tag="K0W68_000481"
     CDS             complement(61729..62259)
                     /gene="rppH"
                     /locus_tag="K0W68_000481"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBI3072150.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            62944..63633
                     /gene="mutH"
                     /locus_tag="K0W68_000482"
     CDS             62944..63633
                     /gene="mutH"
                     /locus_tag="K0W68_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBI3072151.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63702..64415
                     /locus_tag="K0W68_000483"
     CDS             63702..64415
                     /locus_tag="K0W68_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBI3072152.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64553..64771
                     /gene="ygdR"
                     /locus_tag="K0W68_000484"
     CDS             64553..64771
                     /gene="ygdR"
                     /locus_tag="K0W68_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBI3072153.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT        15709 a        17425 c        15889 g        15789 t
ORIGIN      
        1 ggaggctggc atttttaaat cagataaagt cagtcttatg cttggccttt gatctcttta
       61 cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga tacgaatcag ctggttgtat
      121 ttagcaacac ggtcagaacg gctcatagaa ccagttttga tctggcctgc agcagtacca
      181 acagccaggt cagcgatggt agcgtcttca gtttcgccag aacggtgaga gataactgca
      241 gtgtagccag catctttcgc catcttgatt gcagccagag tttcggtcag agaaccgatc
      301 tggttgaatt tgatcaggat ggagttagcg ataccttttt cgataccttc tttcaggatc
      361 ttggtgttgg ttacgaacag gtcgtcacca accagctgga ttttgtcgcc cagaactttg
      421 gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat agatacgatc
      481 ggatactgtt tggtcagttc ttccaggaag tgagtgaatt cttcagaggt gaaagctttg
      541 ttgccttcgc cagccagaac gtatttaccg tctttgtaga attcagaagc tgcgcagtcc
      601 atcgccagag tgatgtcttt gcccagttca taaccagcag ctttaacagc ttcagcgata
      661 acagccagag cttcagcgtt ggaacccagg ttcggcgcat agccaccttc gtcaccaact
      721 gcagtgttca tgcctttcgc tttcagaact tttgccaggt gatggaaaac ttcagaaccc
      781 atgcggatgg cttctttcac agttttcgcg ccaaccggct gaatcatgaa ttcctggata
      841 tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg aaccggcatg
      901 gagtatttgc ccggagtgcc gttcagttca gcgatgtgct cgtacagcgg catcccttta
      961 tcggcagcag cagctttggc gttagccaga gatacagcca ggattgcgtt cgcgccgaat
     1021 ttggatttgt tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc agcctgatct
     1081 ttagcgtctt tgccaatcag tgcctgagcg atcgggccgt ttaccgcagc aacagctttg
     1141 gttacgcctt tacccaggaa acgggatttg tcgccatcgc gcagttccag agcttcacgg
     1201 gaaccagtag aagcacctga cggagcagct gccataccga cgaaaccacc ctccagatgt
     1261 acttcggctt caacagtcgg gttaccacgg gagtcgatga tttcacgacc gatgattttt
     1321 acgattttgg acattaggtt ttcctcaagt cactagttaa actgaaactc cagacaaaca
     1381 acgcgtaccc aaggtacgcg ttgccgttct aactttttta cttacttcgc ctgacgtttc
     1441 tggaactcgc tggcagcttt cacaaagcct gcaaacagcg ggtgaccatc acgtggagta
     1501 gaagtaaact ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg aacttcgatg
     1561 atctcgacca actgatcatc cccggaacgg cccgcaacgc gcagacctgc atcttcaatc
     1621 tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac aattgtcggc
     1681 gcattgtaca gctggcgaac caggctatcg tcaaccaact ggcactgctg tgcgccgaga
     1741 cgcatggtac cgccgagatc gctcttctcg ctacgaactt caacgttgcc gttttcatcg
     1801 cgccactcgg taatcagcgc cacaaccggg tacttacagt ctggcacaaa ttccgtagag
     1861 ttggcgttct ccatgttggc aacatggcga gcgtaatcaa ttaacgccac ctgcataccc
     1921 aggcaaatgc ccagataagg aatattgttc tcacgcgcaa aacgcgcggt cgtaatcatg
     1981 ccttctacgc cacgatagcc gaaaccgcca ggtacgagga ttgcgtccag acctttaagg
     2041 atttcaacgc cgcgcgtttc aacatcttgt gaatcgatca gtttgatgtt gacgctgaca
     2101 cgattcttca gcccaccgtg tttcagtgct tcgatcactg atttataagc atccggcagt
     2161 tcaatgtact tgccgaccat accgatggtg acttcactta ccggattcgc ttcttcgaag
     2221 ataacctgtt cccattcgga cagatttgct tccgggcagt ttaagctgaa tcgtttacaa
     2281 atataatcgt ccagcccctg agatttcaac aggcccggaa ttttatagat ggaatcgacg
     2341 tctttcagag aaataaccgc tttttccgga acattacaga acaatgcaat cttcgcacgt
     2401 tcgttcgccg gaacagcgcg atctgaacga caaatcagga tgtcaggctg gataccgatg
     2461 gagagcagct cttttacaga gtgctgagtc ggtttggttt tgacttcacc agacgctgcc
     2521 atgtacggca ccagcgtcag gtgcataaac agagtgtgct cacggccaat ttcaacagcc
     2581 atctggcgaa tcgcttcgag gaacggcaag gattcgatat cacctactgt accgccgatt
     2641 tctaccagta ctacgtcatg accttcgcca ccttccagca cgcgctcttt gattgcgtta
     2701 gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt caccgcggcg ttctttacgc
     2761 agaacgtcag agtagatacg acccgtggtg aagttgttgc ggcggctcat tttggtacga
     2821 atgaaacgct cgtagtgccc caggtccagg tcggtttcag cgccgtcttc agtaacgaac
     2881 acttccccgt gttggattgg gctcatagta cctggatcga cgttgatgta cggatccagt
     2941 ttcatgatgg tcacattgag gccacgggct tcaagaatgg ctgcgaggga ggctgcggca
     3001 atgcctttac ccagagagga tacgaccccg ccggtcacaa aaatatagtt cgttgtcatg
     3061 ctgaacctga gaagttaggt tgaaaagacg atggaataac caggacggga aagcagtata
     3121 cccgaacatg acctgtgcca caaactttca ttatccctcc tcttcgccag cgcactattg
     3181 aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa atcaagcgct
     3241 tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta attcccttag agatcaattt
     3301 cctgccgttt tacctgttgc cagacttctt ccattgtttc gaggtcaaca cctgtcattt
     3361 ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa acgacgctcg aatttttcgt
     3421 tcgctttttg caatgcgatt tctgctttcg tccctaaatg gcgagccaga ttaaccgtgg
     3481 caaacagcag gtcccccatt tcctcctcca gtttagcctg gtcgacaaca gcctgccgcg
     3541 cctcgtacat cacctcgtcg atctcttcgt agactttatc aaccaccggg ccaagcgtcg
     3601 tccagtcgaa gccaacgttg gcgcaacgtt tctggatttt ttgcgcccgc attaaagccg
     3661 gtaaactacg aggaatatcg tccagcgccg aatgctgcgc tttctgcgcg cgctcttcgg
     3721 ttttgatttg ctcccaacgg gcaagcactt cactactgtt ttcggcagaa ctatcagcaa
     3781 aaacatgcgg atggcgacgc tctaatttat cgctaatagc agcgcaaata tcattaaagt
     3841 caaagcgccc ttcttcctga gccatttgcg cgtaaaacac cacctggaat agcagatcgc
     3901 ccagttcacc gcgaagatcg tcaaaatctt cacgggcgat ggcgtccagc acttcgtagg
     3961 tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc cacgggcagc
     4021 cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa acggtcgatt tgattcattg
     4081 aattgtcctg aaaattgcgg gtctgtcagg tggaaacctg tgaccagaat agaagtgagt
     4141 tagtaacact acccaatcag tacgttaatt ttggctttaa tgagttgtaa ttcctctggg
     4201 gcaactgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt tacctgatca
     4261 gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg atatcctttt
     4321 gattgcgttg tacaaggaac acacagacac atacctgttt tgttgttgta catgaaagga
     4381 ctcaggacaa cagctggacg atgtccagct tgctcgctac cttttgtcgg gtcaaaatca
     4441 acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag acttccttat
     4501 ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg tcgttgacca
     4561 gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat ttgccatcca
     4621 ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt aacgtagccg
     4681 ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc ataacccttt
     4741 cctcaaaccg ctatcatatg tagatacagt atatatcaat ctacattgta gatacgagca
     4801 aatttctgcc taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     4861 tttacccagc acgcggccca gcacctgcag gttgtaaatc tcaatggtca tgtcgatggt
     4921 tgccagttgc tgttttgtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     4981 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     5041 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     5101 cgcatgagag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     5161 cccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     5221 gtggtgcatc aggttgccaa caccttcgac taccacgcga ccgttatctt tactgcggtt
     5281 ttgcggcgtg tagctttttt gctgaagttg cttcagcgcg gcggcgtcct gctcttcggc
     5341 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     5401 gccaccaatc gccgccagca actcgtcgac atcattaaag ttgtaacgcg gcagcagatg
     5461 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5521 cccagccaga atgtttttgt cacggtcctg tttacggaac caagcgtgaa ttttcgaacg
     5581 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5641 ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg tgaacggcac
     5701 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5761 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     5821 gacatacacc cggtcgtcaa agacctggct gcgtacttcg tcgagcattt cgccggaatc
     5881 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     5941 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     6001 ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt tgccacctgg
     6061 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacataatc
     6121 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcatagca
     6181 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcaaaggc
     6241 gaggttcttt ttctgcattt tacgccagat gctgtagatg tgtttcggac gaccatacac
     6301 ttccgcttta acgccttcag ctttcatctc agcgcgcaga tgaccaacga actcttcgat
     6361 gtagtgttcg cggtcgagac gccgttcatg cagcagtttg gcaattcgtt tgtattcggt
     6421 tggatggagg taacggaagc agtaatcttc cagttcccat ttcagttgtc cgattccgag
     6481 acggttagcc agcggtgcgt agatgttggt acactctttt gccgccagta cacgttcatc
     6541 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgactac
     6601 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6661 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6721 gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6781 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     6841 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     6901 cccctgcgtc tgttgcagac aatacgccca agtttcggct aagcactcac acgacttctg
     6961 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     7021 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     7081 gcaaatcgca actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     7141 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     7201 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     7261 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     7321 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     7381 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgcc
     7441 ggaacgcctt ctacacccac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7501 cccataccgc agaacaaatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7561 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7621 aaagttaagc acaacccatt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7681 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7741 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7801 gttgcctgta ccagttcaac atgaccaaga tggcgcataa cttgtaagct gcccaagcat
     7861 gccatgactt tgggcagcaa tgcttcaagt tggggcacta aaatggggca ttgtttaacg
     7921 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     7981 aagtagttca gacttaaacg cgcgcgacgg cgatagcccc agggaacatc ggcgatcact
     8041 tcagagactt cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     8101 tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg acagcgtggc
     8161 gtttcgcgtc ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     8221 ttatcttcag taacagtaac ttccgcgttt tcctgcggca ataatccggg gataaatagc
     8281 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     8341 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     8401 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     8461 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8521 gctgagtatc tttttcgttg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8581 cggagccagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8641 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8701 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8761 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     8821 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     8881 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgctggagct
     8941 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcca gcgtgatgat
     9001 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     9061 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     9121 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     9181 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     9241 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttagatc tggcgaaaaa
     9301 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     9361 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     9421 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9481 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc tggaaagtat
     9541 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9601 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9661 cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9721 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9781 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
     9841 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccggtct
     9901 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
     9961 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
    10021 tattatcgaa gggccatcca cccagtgcct cgccggtaag cgcctggcct atgtcgaacc
    10081 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
    10141 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
    10201 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
    10261 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagtcaatg cagaaaaact
    10321 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc gcctgttgcc
    10381 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
    10441 caagctggca atgacagtca tggcagttga tgacaacccc gctaacctga aacttatcgg
    10501 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcggt
    10561 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgcctgacat
    10621 ggatggcatt cgggcctgcg agctcatcca ccagctcccc catcagcaac aaacgccggt
    10681 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10741 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10801 caagcctggc agcggtattt cctctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    10861 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    10921 tctagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    10981 tgaggagcag ctggttggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    11041 tggcagttgc ggctatagcg gtgtgccgcg tatgaagaat ctctgccaac tgatcgaaca
    11101 acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg aactgttgga
    11161 cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga cgtcttgtcg
    11221 agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc ctgtgtttat
    11281 gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc aatgaattaa
    11341 aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc atcagtctca
    11401 agccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg cacgggcgct
    11461 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    11521 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    11581 cacccagccc cggtttttcc ggcacctgaa ccagaccgcc tttgatctca aacggttctt
    11641 tggtcaagcg ctgattgcct tcctgccaaa tccagtgcgt atcaatcgcg gtgattttac
    11701 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgtttg
    11761 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    11821 gcatagtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    11881 ggcccatctg ccgccagtcg gtggcgatca tattggttgc agtcggcagg ccagtcgcgc
    11941 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca tacggatctt
    12001 ctgcataagc cagcgaacct ttcaggtatt taccaatttt aattgcttcg ttcagcgacc
    12061 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa
    12121 tagactcggc ttcttcttcc ccggccagta caccgccttt cagtttgaaa tcgttgaagc
    12181 catatttttc atacgccgct tccgctaggc gcaccaccgc atccggcgtc atcgcttctt
    12241 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagaggtg
    12301 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    12361 gaccatcgcc cagcagcgat gccacgttta cccccagatg ctgccccagc agatccagca
    12421 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    12481 cgcgcccacc agcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    12541 attcacccag cgttttacct accaccagcg gaatcgcatc ttccagcgtt ttacggattt
    12601 tctcgccgcc gggaatttcc cctacgccag tgtgaccgga attatctttg ataatcacaa
    12661 tattacgcgt aaagaacggt gcgtgtgccc cactcagatt catcagcata ctgtcatgac
    12721 ccgccaccgg gatgacctgc atttcagtaa caacagacgt cgtaaattga gaactcatat
    12781 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    12841 gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgccag gcagacgttt
    12901 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    12961 atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca ggcgacaatc
    13021 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    13081 cacatgggta aacatcgcca gagagatatc gaaatggtta ttagaatggc agccccaggt
    13141 cagcccccag tcgtcgcaaa gctgcgccac acggactgca ccggaaagcg tccagaagtg
    13201 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    13261 gttggtggcg atcatgttag tcgcgacggg caagccggtc gcccgccgaa actccgccat
    13321 aacttcacga ccggagaaac cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    13381 atcattcagc cctttgcata gagaaatggc ttcatcaagt agccaggcac cgttgggatc
    13441 aacggtaatc cgcgcatcag ggaagcgttt cttcaatgca cgaacagtgt cgatttcttg
    13501 ctcgccaggt aacaccccgc ccttaagttt gaaatcttta aagccatagc gatcctgtga
    13561 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    13621 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    13681 gatataaaac agataaccga ggacggtaat agtctcgcgt tgcttgcctg gccccaacag
    13741 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    13801 cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg tatcaaaatc
    13861 ggctgcctga ttacctttat gcacctgctg aaccacttta ttcaggcgcg caacttcctg
    13921 gcccagaacc atcggaatag catcgaccag tgtttgataa atcacctctc cgcccggcgc
    13981 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acaatattgc gggtgaaata
    14041 tgcgttatgt gccccaccaa tattaagcaa catgctatcg tgcccggcga ccggaatgac
    14101 tttcatatca gtaataacag gactggattg tgtcgtcatc ttattgcccc gcgacaggtt
    14161 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaagg
    14221 catgaacacc aacataaatc agcgccccat taaaggagcc agtcgtgcca acgatataac
    14281 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    14341 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    14401 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    14461 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    14521 caatgttcag cgatcccgtg cgacgcatca gccagtcgga aataatccca cccagcacac
    14581 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    14641 tcccacgcgc ctgcaccaga taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt
    14701 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agttgtttga
    14761 tctgccccca cttcacgctg aacggcactt taactttggt gttttgctga tccatattga
    14821 tcagcgcgcc acccgcggcg atgtactcca gctctttctg atttaccccc ggatgttgat
    14881 ttggctcgtg gatgactttc aaccagataa agctgatgac aatccccaga ccgcccataa
    14941 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    15001 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgcg
    15061 ccggaaacca ggccgcaaca atgtgactgt tgccggggaa agatggcgct tcagcaagcc
    15121 cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta aagatatcga
    15181 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    15241 ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca taagcccatg
    15301 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    15361 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    15421 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    15481 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    15541 gacatagcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    15601 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    15661 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    15721 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggttaaaacg
    15781 aagaaataaa gaagagtatg aaatggatcg cttgactcca ggcaaacgcc agtaaaaatc
    15841 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    15901 aacgacattt accgctcgct gaacatatca ggacaacagc gtgccccact gttcgaccca
    15961 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16021 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcagaa
    16081 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16141 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16201 ggtcgtggac gtaaccacca gaacatactt atcctgataa ggcagccagt cgcttaattc
    16261 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16321 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16381 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16441 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16501 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16561 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16621 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16681 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16741 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16801 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    16861 tccaccagtg gataatccag caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    16921 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    16981 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17041 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17101 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17161 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17221 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17281 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17341 tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17401 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17461 cttcaagcgt ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacttcaat
    17521 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17581 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17641 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17701 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17761 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    17821 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    17881 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    17941 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18001 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18061 gcttatttcc tggcgatctc ctacagggtc tgtaagagat ctctcacaac cagatggggt
    18121 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18181 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18241 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18301 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18361 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18421 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18481 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18541 tagttgaaat aatgcacggt gaaggaacac cgtacagttc ctcacttaac ggccagcttt
    18601 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18661 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18721 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18781 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    18841 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    18901 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    18961 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19021 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19081 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19141 ttgatgaact ggaaggccag ccgataggtc attttaatgg cacttgcatt gatgaccagg
    19201 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc acctgtggtg
    19261 aaaaagtagt ggaagagacg cttgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19321 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19381 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19441 aacgcatctt taatgacctg ttacgcttct gccagccaga aaaattgagc gtttacgcac
    19501 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19561 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19621 gcagaaggtt gtgaaaggac atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19681 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19741 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19801 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    19861 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    19921 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgcgttgc
    19981 aggccaactt gttcgcggtt ctgcgtgaca ttctcttcgt ttacgggcaa atccataaca
    20041 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20101 ccatcttgcg taacgctcgc gcactgcatg tcggtgaagc gccaaatatg gtggtctgct
    20161 ggggcggaca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaatcagc
    20221 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc gggagcgatg gaagcgccga
    20281 tgaaaggtgc tgcggtcgga cacgcacagc agcgttacaa agacagtcgt tttattggta
    20341 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20401 tcatgccaga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20461 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20521 acccggccaa caaagatcag gttttaccat tgttcctcac cggcccgaaa gagagcgccg
    20581 actacttccg cgtactggac gagtttgtcg tgcatacgtt gggtgaaaac gcgcgccgcc
    20641 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20701 cgctggtgaa agaaaatcgc cgcgacacag gcgatgccta cagctttaac tggtcaatgc
    20761 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    20821 agctttaccc ggatcaacct gttgaagtgc tggctgccga tctgcgccgt gcgttctccg
    20881 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    20941 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21001 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21061 cgtaatctcc ctcccccggt gacgtttgtt gccgggtttt ctcgtttttg gtcacatacc
    21121 catcaattta tttcatttgt tatatgaatg tttcttacca ccctcacgcg ataaatatca
    21181 ttgcagttaa tatgtcatac aatttatgtt gtaacgcaaa cgtttcccta ttttcataaa
    21241 ccacatttta tcgctatgaa aaagaaatta tcgccatgat taactaaaag tattgatttt
    21301 ttcagttcaa cctacatata ttgcgcgccc cggaagaagt cagatgtcgt ttaatgggca
    21361 aatattgccc ttaaattctc ttttactttt gatttacaga gtaaagcgtt gggataatct
    21421 atcttccaag tagattattg tatttgggat caagatcact gatagataca taacttgtgt
    21481 gtatctttcc gccctcaaat tattacggcg gtaaatgatt aagccatcgc cgatagacag
    21541 atttcatttt tacggtcagg caccttcccg agctgaactg gctaaaagct gaattatttg
    21601 cattcctcca ggagaaatag atggaaacga ctcaaaccag cacgattgcg tcgaaagact
    21661 ctcgtagtgc ctggcgcaag acagacacca tgtggatgct gggcctttac ggcacggcaa
    21721 tcggcgcggg cgtgctgttc ctgccaatca acgccggtgt tggcggtatg atcccgctga
    21781 tcatcatggc tatccttgcg ttcccgatga cattttttgc tcaccgcggc ctgactcgct
    21841 tcgtactgtc tggtaaaaac ccaggcgaag acatcaccga ggttgtagaa gaacactttg
    21901 gtattggcgc aggtaaactg attaccctgc tctacttctt cgctatctac ccgatcctgc
    21961 tggtttatag cgtggcaatc accaatacag ttgaaagctt catgtctcac cagctgggta
    22021 tgacaccacc gccgcgtgcg attctgtcgc tgatcctgat cgtgggtatg atgaccatcg
    22081 ttcgcttcgg tgagcagatg atcgttaaag caatgagtat tctggtattc ccgtttgttg
    22141 gcgtactgat gctgctggct ctgtacctga tcccgcagtg gaacggcgca gcactggaaa
    22201 cgctgtctct ggacactgca tctgcaaccg gaaacggtct gtggatgacc ctgtggctgg
    22261 caattccggt tatggtgttc tctttcaacc actctccgat catctcttct ttcgccgttg
    22321 cgaagcgtga agagtacggc gatatggcag aacagaaatg ctcgaagatc ctggcattcg
    22381 cacacatcat gatggtgctg accgtaatgt tcttcgtctt cagctgtgta ctgagcctga
    22441 ctccggcaga cctggctgcg gctaaagagc agaacatctc gattctgtct tacctggcta
    22501 accactttaa cgcaccggtt atcgcgtgga tggctccgat tatcgcgatt atcgctatca
    22561 ccaaatcctt cctcggccac tacctgggcg cacgtgaagg cttcaacggt atggtgatta
    22621 aatctctgcg tggtaaaggt aagtctatcg aaatcaacaa gctgaaccgt atcactgcgc
    22681 tgttcatgct ggtaacgacc tggattgttg ccaccctgaa cccgagcatc ctgggtatga
    22741 ttgaaaccct gggcggccca atcatcgcga tgatcctgtt cctgatgccg atgtacgcaa
    22801 ttcagaaagt accggcaatg cgtaagtaca gcggtcacat cagcaacgta ttcgttgtcg
    22861 tgatgggtct gattgcaatc tccgcaatct tctactctct gttcagctaa gtcctttcgc
    22921 gccgctttcg ggcggcgctt cctccgtttt aacacgatgt atttcctatg attagcgtat
    22981 tcgatatttt caaaatcggc attggccctt ccagttctca taccgttgga ccaatgaaag
    23041 cgggtaaaca atttaccgac gatctgattg cccgtaatct gcttaaagac gtgacccgcg
    23101 tggtggttga cgtgtacggc tcgctctctc tgaccggtaa aggccaccac actgatatcg
    23161 ccattattat gggcctggcg ggtaacctgc cggataccgt ggatatcgat tccatccctg
    23221 gttttattca ggatgtgaat actcatggtc gcctgatgct ggcaaacggt cagcatgaag
    23281 tggagttccc ggttgatcag tgcatgaact tccatgcaga caacctttct ctgcatgaaa
    23341 acggtatgcg cattaccgcg ctggcgggcg ataaagtcgt ttacagccag acttactact
    23401 ctattggcgg tggctttatc gttgatgaag agcattttgg ccagcagaat agcgcaccgg
    23461 ttgaagttcc ttatccgtac agttcagcag ccgatctgca aaaacattgt caggaaaccg
    23521 ggctgtcact ctctggcctg atgatgaaaa atgagctggc gctgcacagc aaagaagagc
    23581 tggaacagca cctggcgaac gtatgggaag tcatgcgtgg cggtattgag cgcggtattt
    23641 ccaccgaagg cgtgttgcct ggcaaactgc gcgttccacg ccgtgctgcg gcactacgcc
    23701 ggatgctggt cagccaggat aaaaccacca ctgacccgat ggcggttgtt gactggatca
    23761 acatgtttgc actggcagtg aacgaagaga acgctgctgg cggacgcgtg gtgactgcgc
    23821 cgactaacgg tgcgtgcggg attatcccgg cagtgctggc gtactacgac aagttcatcc
    23881 gcgaagtgaa cgctaactca ctggctcgtt acctgctggt agccagtgcc attggttctc
    23941 tttataagat gaacgcgtca atttctggtg ctgaagtcgg ctgccagggt gaagttggtg
    24001 tggcgtgctc aatggcggcg gctggtctgg cagaactgtt aggtgcaagc ccggcgcagg
    24061 tgtgcatcgc ggcggaaatc gccatggagc acaacctcgg tctgacgtgt gacccggtcg
    24121 ccggacaggt ccaggtgcca tgcatcgagc gtaacgccat tgcggcagta aaagcggtga
    24181 acgcctcacg tatggcgcta cgccgtacca gcgagccgcg cgtctgcctc gataaagtta
    24241 tcgaaaccat gtacgaaaca ggtaaagata tgaacgccaa gtaccgcgaa acctctcgcg
    24301 gcggcctggc aatgaagatc gttgcctgcg attaatcgct ctccagaggc ctcgttttgc
    24361 gaggcctctt cccgatttct catccagccg tagcctgttc cggcatcgaa tgttaccctt
    24421 atcgcctgat ctttaagggg gttatcgtgg ctgttcattt gcttattgtc gatgcactga
    24481 atcttattcg tcgcattcat gccgttcagg gatcgccctg tgtcgaaacc tgccagcatg
    24541 cgctcgatca gctcattatg cacagccagc caacccacgc ggtcgccgtt tttgatgatg
    24601 aaaaccgcag tagcggctgg cgtcatcagc gtttaccaga ttacaaagcg ggtcgaccgc
    24661 caatgccgga agagttgcac gacgagatgc ctgcattacg cgccgccttt gagcaacgcg
    24721 gcgtcccgtg ctggtcaacc agcggcaacg aagccgatga cttagccgcc acgctggcgg
    24781 tcaaagtgac acaggccggg catcaggcaa cgattgtttc gacagataaa ggctactgtc
    24841 agttactttc accgacatta cgtattcgtg attacttcca gaaacgttgg ctggatgcgc
    24901 catttatcga taaagaattt ggcgttcaac cgcagcagtt gcccgattac tggggacttg
    24961 cggggatcag cagttcaaag gtaccgggtg ttgcgggaat cggaccaaaa agcgccacgc
    25021 agctgctggt cgagtttcag agtctggaag ggatatatga gaatctggat gcggttgccg
    25081 aaaagtggcg caaaaaatta gaaacccata aagagatggc gtttctgtgc cgcgatattg
    25141 cccgcttaca aaccgatttg catatcgacg gcaatttaca gcaattgcgg ttggtacggt
    25201 aacggcgagc cggatgcaaa cgattatcaa cacattgaat ttaatgcact tatataccgg
    25261 ataagacgcg gctagcgtcg tatccggcat tatcacatca gcgcatttac caggcggtat
    25321 ggtaaagctc tacaatatcc tcaagcgttg cttcacgcgg gttgccaccg gtacaaacat
    25381 catccagtgc cgcctgcgcc agtgccggaa tgtcttcctt gcgtacacca acatcacgca
    25441 aatgtggcgg aataccgaca tcacggttga gagcaaacac cgcttcaaca gcggcattac
    25501 gcgcctcttc caggctcata ccttccactt tcacgcccat aacgcgcgcg atatcgcggt
    25561 acttctcacc ggtaaagtca gcgttatagt gcatgacatg cggtaacagg atggcgttcg
    25621 caacaccgtg tggagtgttg taaaacgcgc ccagtggatg cgccatacca tgcaccaacc
    25681 ctaacccaac attcgagaag cccatacccg caacatactg cccgagcgcc atttcttctc
    25741 cggcatcctt atcaccagca accgatcctc gcagcgcccc agcaatgatt tcaatcgctt
    25801 taatgtgcag tgcatcggtt agcgcccacg cgccacgggt aatatacccc tcaatagcat
    25861 gagtgagcgc atcgacaccc gtcgcagctt tcagcgctgg aggcatacca tccatcatgt
    25921 cagcgtcaat aaacgccacc tgcgggatat catgcggatc aacgcaaaca aacttgcgcc
    25981 gtttctcttc gtcagtgatc acgtagttaa tggtcacttc tgccgcagta cctgctgtgg
    26041 taggaattgc cagaatcggt acactgggtt tattggtcgg ggaaagccct tccaggctac
    26101 gcacatcggc aaactccgga ttgttgctga taatgccaat cgctttacaa gtatcctgtg
    26161 gagaaccacc accaatagcg atcaggtaat ccgcgccgct attctggaat acaccgagcc
    26221 cttctttgac gacagtaatt gttgggttgg gcactacgcc gtcgtaaatc gcccatgcca
    26281 gccctgcagc atccatctta tcggtcactt tcgccaccac gccgcattgc accagcgttt
    26341 tatcggtgac gatcagcgcc ttctgataac cacggcgttt cacctcatcg gttaaagccc
    26401 caacagcacc ccgaccaaac catgccgttt cgttcagaat cattctgtta gccatcatcc
    26461 ttctccttgt tgctttacga aattactctt caatccgtaa cccataggtt ttgaatttct
    26521 ccagcactac ggcaatctct tcatcgctca gcactggcac cgggtccgta atcgccaggg
    26581 tcgccacgta aagttgcgcc agcacttcaa cttcatgcgc cagccataac gctttttcca
    26641 gattcacctc acaagcgata agcccatgat gttgtaacaa agttgcctta cgatttttga
    26701 gagccagcgc aacatgttca gaaagttcgc gtgttccaaa ggtcgcataa ggcgcgcaag
    26761 gaatagaatt accacctgcc gccgcaatca tgtagtgaat agcggggatc ggtcggttaa
    26821 gaatggaaac tgctgtgcaa tgaacggcat gattgtgaac aaccgcgttg gcatccggtc
    26881 tgctttgata ggccgccata tggaaacgcc attcgcttga ggggagcttt ccttcctcat
    26941 gtttaccgtt gccatcaata aagacaatat gcgactccgt cagtttttca tatggaatgc
    27001 ctgtaggcgt aatcagcatc ccatcctgat aacgtacact gacgttcccc gctgtcccct
    27061 ggttcagtcc caggcgggtc atttccaggc aagtgtcaat aatctgacga gcaagtttat
    27121 ttcgttccat tagctacctc tctctgattc aaaacagggc aataatgttg ttcctttcac
    27181 actattgaat tagccgttta attacccacc atcttcttcc tgattaacaa gaaagaaatt
    27241 cacaagctta tattttgtga cctagctcaa ctaatcacag taaataactg caagttctct
    27301 ttttataacc ccattaaaaa tgaccgctct taaaaatatt tatcaaaacg gtcatttttc
    27361 tattcaccca agccaggaat gcccgttttc ggcataaaca attaatacag tcatctggtt
    27421 tgtgtttttt atgatttatt ttctgaaacg ggcatgaaat ttcgattatt aaagtgatgg
    27481 tagtcacata aagtcacctt ccagctaata agtgtgaccg ccgtcatatt acagagcgtt
    27541 ttttatttga aaatgaatcc atgagttcat ttcagacagg caaatattca ctgatatgaa
    27601 gcccgaactc gctggttttg cacttttgaa aacataaccg attacgtgct taagcttctg
    27661 aacctaagag gatgctatgg gaaacacatc aatacaaacg cagagttacc gtgcggtaga
    27721 taaagatgca gggcaaagca gaagttacat tattccattc gcgctgctgt gctcactgtt
    27781 ttttctttgg gcggtagcca ataaccttaa cgacatttta ttacctcaat tccagcaggc
    27841 ttttacgctg acaaatttcc aggctggcct gatccaatcg gccttttact ttggttattt
    27901 cattatccca attcctgctg ggatattgat gaaaaaactc agttataaag caggaattat
    27961 tactgggtta tttttatatg ccctgggcgc tgcattattc tggcccgctg cagaaataat
    28021 gaactacacc ttatttttag ttggcctatt tattattgca gccggattag gttgtctgga
    28081 aactgccgca aacccttttg ttacggtatt agggccggaa agcagtggtc acttccgctt
    28141 aaatcttgcg caaacattta actcgtttgg cgcaattatc gcggttgtct ttgggcaaag
    28201 acttattttg tctaacgtgc cacatcaatc gcaagacgtt ctcgataaaa tgtctccaga
    28261 gcaattgagt gcgtataaac acagcctggt attatcggta cagacacctt atatgatcat
    28321 cgtggctatc gtgttactgg tcgccctgct gatcatgctg acgaaattcc cggcattgca
    28381 gagtgataat cacagtgacg ccaaacaagg atcgttctcc gcatcgcttt ctcgcctagc
    28441 gcgtattcgc cactggcgct gggcagtatt agcgcaattc tgctacgttg gcgcacaaac
    28501 ggcctgctgg agctatttga ttcgctacgc tgtagaagaa attccaggta tgactgcagg
    28561 ctttgccgct aactatttaa ccggaaccat ggtgtgcttc tttattggtc gtttcaccgg
    28621 tacctggctc atcagtcgct tcgcaccaca caaagtcctg gccgcctacg cattaatcgc
    28681 tatggcactg tgcctgatct cagccttcgc tggcggtcat gtgggcttaa tagccctgac
    28741 tttatgcagc gcctttatgt cgattcagta cccaacaatc ttctcgctgg gcattaagaa
    28801 tctcggccag gacaccaaat acggttcgtc cttcatcgtt atgaccatta ttggcggcgg
    28861 tattgtcact ccggtcatgg gttttgtcag tgacgcggcg ggcaacatcc ccactgctga
    28921 actgatcccc acactctgct tcgcggtcat ctttatcttt gcccgtttcc gctctcaaac
    28981 ggcaactaac tgaacatatt ttccgaataa agtgaggaat ctgtaatgaa aaaaatcagc
    29041 ttaccgaaaa ttggtatccg cccggttatt gacggtcgtc gcatgggtgt tcgtgagtcg
    29101 cttgaagaac aaacaatgaa tatggcgaaa gctacggccg cactgctgac cgagaaactg
    29161 cgccatgcct gcggagctgc cgtcgagtgt gtcatttccg atacctgtat cgcgggtatg
    29221 gctgaagccg ctgcttgcga agaaaaattc agcagctaga atgtaggcct caccattacg
    29281 gtaacgcctt gctggtgcta tggcagtgaa accatcgaca tggatccaac ccgcccgaaa
    29341 gccatttggg gctttaacgg cactgaacgt cccggcgctg tttacctggc agcggctctg
    29401 gcagctcaca gccagaaagg catcccggca ttctccattt acggtcatga cgttcaggat
    29461 gccgatgaca catcgattcc tgccgatgtt gaagaaaaac tgctgcgctt tgcccgcgcc
    29521 ggtttggccg tcgccagcat gaaaggtaaa agctatctgt cgctgggcgg cgtttcgatg
    29581 ggtatcgccg gttctattgt tgatcacaac ttctttgaat cctggctggg aatgaaagtc
    29641 caggcggtgg atatgaccga actgcgtcgc cgtatcgatc agaagattta cgacgaagcc
    29701 gaactggaaa tggcactgac ctgggctgat aaaaacttcc gctatggcga agatgaaaat
    29761 aacaaacagt atcaacgcaa tgccgagcaa agccgcgcag ttctgcgcga aagtttactg
    29821 atggcgatgt gtatccgcga catgatgcaa ggcaacagca aactggccga tattggtcgc
    29881 gtggaagaat cacttggcta caacgccatc gctgcgggct tccaggggca acgtcactgg
    29941 accgatcaat atcccaatgg tgacaccgcc gaagcgatcc tcaacagttc atttgactgg
    30001 aatggcgtgc gcgaaccctt tgtcgtggcg accgaaaacg acagtcttaa cggcgtggca
    30061 atgctaatgg gtcaccagct caccggcacc gctcaggtat ttgccgatgt gcgtacctac
    30121 tggtcaccag aagcaattga gcgtgtaacg gggcataaac tcgatggact ggcagaacac
    30181 ggcatcatcc atttgatcaa ctcaggttct gctgcgctgg acggttcctg taaacaacgc
    30241 gacagcgaag gtaacccgac gatgaagcca cactgggaaa tctctcagca agaggctgac
    30301 gcttgcctcg ccgctaccga atggtgcccg gcgatccacg aatacttccg tggcggcggt
    30361 tactcttccc gcttccttac cgaaggcggc gtcccgttca ccatgactcg tgtcaacatc
    30421 atcaaaggcc tgggatcggt actgcaaatc gcggaaggct ggagcgtgga attgccgaag
    30481 gatgtgcatg acatcctcaa caaacgcacc aactcaacct ggccaaccac ctggtttgca
    30541 ccgcgcctca ccggtaaagg gccgtttacg gatgtgtact cggtaatggc gaactggggc
    30601 gctaaccatg gggttctgac catcggccac gttggcgcag actttatcac tctcgcctcc
    30661 atgctgcgta tcccggtatg tatgcacaac gttgaagaga ccaaagtgta tcgtccttct
    30721 gcctgggctg cgcacggcat ggatattgaa ggccaggatt accgcgcttg ccagaactac
    30781 ggtccgttgt acaagcgtta atacattact ccctgatgtg atgcccggtc gctccggcta
    30841 ccgtgcctga acaagcaaga gtggttagcc ggataagcaa tgttatccgg ctatattgca
    30901 ggagcgatta tgaaacaaga agttatcctg gtactcgact gtggcgcgac caatgtcagg
    30961 gccatcgcgg ttaatcggca gggaaaaatt gttgcccgcg cctcaacgcc taatgccagc
    31021 gatatcgcga tggaaaacaa cacctggcac cagtggtctt tagacgccat tttgcaacgc
    31081 tttgctgatt gctgtcggca aatcaatagt gaactgactg aatgccacat ccgcggtatc
    31141 gccgtcacca cctttggtgt ggatggcgct ctggtagata agcaaggcaa tctgctctat
    31201 ccgatcatta gctggaaatg tccgcgaaca gcagcggtta tggacaatat tgaacagttg
    31261 atctccgcac agcggttgca ggctatttct ggcgtcggag cctttagttt caatacgtta
    31321 tataagttgg tgtggttgaa agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg
    31381 ctctttattt cgtcgctgat taaccaccgt ttaaccggcg aattcactac tgatatcacg
    31441 atggccggaa ccagccagat gctggatatc cagcaacgcg atttcagtcc gcaaatttta
    31501 caagccaccg gtattccacg ccgactcttc cctcgtctgg tggaagcggg tgaacagatt
    31561 ggtacgctac agaacagcgc cgcagcaatg ctcggcttac ccgttggcat accggtgatt
    31621 tccgcaggtc acgataccca gttcgccctt tttggcgctg gtgccgacca aaatgaaccc
    31681 gtgctctctt ccggtacatg ggaaatttta atggtccgta gcgcccaggt tgatacttcg
    31741 ctgttaagtc agtacgccga ttctacctgc gaactggata gccaggcagg gttgtataac
    31801 ccaggtatgc aatggctggc atccggcgtg ctggaatggg tgagaaaact gttctggacg
    31861 gctgaaacac cctggcaaat gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat
    31921 ggtgtgaaaa tgcagtgtga tttattgtcg tgtcagaacg ctggctggca aggagtgacg
    31981 cttaatacca cgcgggggca tttctatcgc gcggcgctgg aagggttaac tgcgcaatta
    32041 cagcgcaatc tacagatgct ggaaaaaatc gggcacttta aagcctctga attattgtta
    32101 gtcggtggag gaagtcgcaa cacattgtgg aatcagatta aagccaatat gcttgatatt
    32161 ccggtaaaag ttctcgacga tgccgaaacg accgtcgcag gagctgcgct gttcggttgg
    32221 tatggcgtag gggaatttaa cagcccggaa gaagcccgtg cacagattca ttatcagtac
    32281 cgttatttct acccgcaaac tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac
    32341 aatttcgccg ttaatttctc ccgaactatt gaaagtgctg gcagagatgg gacatggaga
    32401 tgaaattatt ttttccgatg ctcactttcc cgcccattcg atgggaccgc aggtgatccg
    32461 cgctgatggc ctgttggtga gcgacttgct ccaagcgatt atcccattat ttgagctgga
    32521 cagttatgcc ccgccgctgg tgatgatggc agcggtagaa ggtgacactc tcgatcctga
    32581 agtagaacga cgttaccgta atgcgctttc actacaagcc ccgtgtcctg acatcatccg
    32641 catcaatcgt tttgcgtttt atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg
    32701 cgaacgagcg aagtacggga atattctttt aaaaaaaggg gtaacaccgt aatctcatat
    32761 cggtacgccc gcatgacgcg ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc
    32821 acgccagcaa gcgatagtcg acctgctgct gaaccatacc agcctgacca cggaagctct
    32881 ctctgaacag ctaaaggtca gtaaagaaac cattcgtcgc gatctcaatg aattacagac
    32941 gcagggtaaa attctgcgca atcatggacg cgctaaatat atccaccgtc aaaatcaaga
    33001 cagtggcgat ccctttcaca tcaggctgaa aagccattat gcgcataaag cagatatcgc
    33061 gcgcgaggcg ctcgcgtgga ttgaagaagg gatggtgata gccttagacg ccagttcaac
    33121 ttgctggtat ctggcacgcc agttgcctga catcaacatt caggtcttca ccaatagcca
    33181 tccgatttgc catgaactcg gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac
    33241 acttgagcgc aaatatggct gttacgtcaa tccctcgctg atttcccaac ttaaatcgct
    33301 ggaaatcgat ctgtttattt tttcttgtga agggatcgat agcagcggcg cactgtggga
    33361 ctccaatgcg atcaacgctg attacaaatc gatgctatta aaacgtgccg cgcaatcgtt
    33421 gttattgatt gataaaagta aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga
    33481 tgaggtaacg cacattattt ctgatgagcg ccaggttgca acttctttgg taacagcctg
    33541 acggaaaggg tagcaggccg aagacgacgc cccggccttg cctgttatcg ctcgtcgcga
    33601 cgaccaccca ccgcagccca gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga
    33661 tacaactgcc gcgcctgaat ctgagcattt atgccatgtt catcaagctg tgcctgaata
    33721 tacgccagat tgtgtgatac ttcttcgtag cgttttttca tcggcagttt gagattgaaa
    33781 atggtttcac ggcaccagcc attaaccagc cactgcgcca tcaatgccgc aactttagcc
    33841 ggtttttcaa ccatatcgca taccatccag gagatattgc tgcgtgtcgg acggaatttg
    33901 aaaccgtctt cccgcagcca cgtcacctgc ccggtatcca tcagactttg cgccatcggg
    33961 ccgttgtcga cggaataaac ccacatgttg cgcttcacca gttggtaggt ccagccgcca
    34021 gggcaagcgc ctaaatccac cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc
    34081 gcaggaataa acacatgaaa tgcctcttcc agtttgagcg tggaacgact cggcgcctct
    34141 gccggaaatt tcaggcgcgg aatgcccata tagaacggcg aattattgtt gctgtatgag
    34201 taaccggtat agcagcagcc tggtgcaata aagaatacat gcacaaccgg acgcttcggc
    34261 gtttcatagt tcgccagcac cccggcatcg cgcaaggcag cgcgtagcgg cacggtgaat
    34321 ttacggcaga atttcagcaa ctctttgctt tcgttggtat cggcaacttc aacacgcagt
    34381 tcaccgccct tctctactac gccctgcaac atgtcgacaa tgggggtaat acgatcttct
    34441 ggcggcaaat gctgcaagag ttcccccacc acaaaccact ggcgggcaaa aattaatgaa
    34501 ctgaacggca gctcacggat taacttatcg ccatcatcag gttgataaca ttcataaatg
    34561 acataacccg cattctcttt cacgcgggca aaaccgaaaa tttcccgctg gccggcttta
    34621 tcggtaattt ctgcggcgca ctctttttca aagcccggac ggcacagcaa tacaacctta
    34681 ttcatgactt acgcccttgc gctttaaacg gatagcacca actaacatta acgcccagcc
    34741 cgcgaggaag ctcacgccgc caaccggtgt gacaaatgcc cacaaacgca gatgggacag
    34801 cgccaggcaa taaaggctgc cgctgaacaa caccgtgcct aacgcgagga aaacgctact
    34861 ccagtaaaac cagatactga tgcgacgctg cattgccacc gccagaccta agatcgccag
    34921 cgtatgaaac gcctggtatt cgaggccggt ctggatccag cccatctcaa cggcccccat
    34981 ggttttactt aacacatgcg cgccaaaagc gcccagagcc acaaaaatga agccgctaat
    35041 ggcggcgaaa atcagcataa aacggctggt catggtcgta ccctacgtaa attattgttc
    35101 ataacgaaag cggaattttt cttgttcagc agcggctttc gccaggatcc attggcgaaa
    35161 ggcggctatt ttacccagtt ctgcctggct gtcatgacaa accagataaa aagcgttttt
    35221 actgaccaga acatcattaa acgggcaaac aagacgtccg gcctcgattt cagattgcgc
    35281 catcacgtta tttgccagcg ccactccctg cccgtgaata gccgcttgca gcaccatggc
    35341 gctatgacta aaaattggcc cttgctgaac gttgatatga tttaacccca actgtcgggt
    35401 atatgtctgc cagtcacggc gtgacgcatc atgtaataac gtatgtttag ccagatcttc
    35461 cggggtcttc aagggttttt cgcctgtcag cagtagcggc gaacacaccg gcaataaata
    35521 ttcggcgtac agtttttcca cccgtagccc cggccagttg ccccgaccat aaaatatcgc
    35581 cacatcaaca tcatccgcca gcttatcttc ctgacgatca accgcctgga ttcgaacgtc
    35641 aattcccgga taagctgaat taaagctgga aagtcgcgga accaaccaat gaatggcgaa
    35701 actggggagt aaactgaccg tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt
    35761 cgcttcggtt aattgcgaaa atatctcttt gatatcgagg aaatagcttt gcccttcctc
    35821 ggtcagcagg agtgaacgat tacggcggcg gaacagtttt agccccaaaa aatcctcaag
    35881 agacttgatt tgatgactta ctgcggcctg ggtcacgaaa agctcttctg ctgcgcgagt
    35941 gaaacttaaa tggcgtgctg cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg
    36001 tttagacatg gcttttaaac tttgatgtta aattaattta acaattagat cacactgtgt
    36061 aacctattag tttttttaat ctgagccatt ataaattgtc cgttgagctt ctaccagcaa
    36121 atacctatag tggcggcact tcctgagccg gaacgaaaag ttttatcgga atgcgtgttc
    36181 tggtgaactt ttggcttacg gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga
    36241 ttcagaccat ggtagcaaag ctaccttttt tcacttcctg tacatttacc ctgtctgtcc
    36301 atagtgatta atgtagcacc gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat
    36361 tactgatcca gttcgaccat ctctttcacg tcagtacggt tgatctgctg tttgttgcca
    36421 ttagcgtctt tatacgaaat cataccggta tcgttatcag tctgtggttt gccgtcagag
    36481 acgatggtac gtccgtcatt ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg
    36541 gcaaaagtca gcatacaggc agaaataatt gcggcagtct ttttcatcat cttctcctta
    36601 aagcgattaa taaatcttaa tagtattgat taacaggcta aaattaacgc ctaacactat
    36661 tcagcatatg ttacttacgc ggcttcgcca ggatatccag ataattctga tggttagcac
    36721 tctccttgta tcaaagtgaa ttttgcgtca cgatcggtgc atcaagccga ggagtaccat
    36781 gaacgttttt aatcccgcgc agtttcgcgc ccagtttccc gcactacagg atgcgggcgt
    36841 ctatctcgat agcgccgcga ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca
    36901 gttttacagc ctgagcgccg gaaacgtcca tcgcagccag tttgccgaag cccaacgcct
    36961 gaccgcgcgt tatgaagctg cacgagagaa agtggcgcaa ttactgaatg caccggatga
    37021 taaaactatc gtctggacgc gcggcaccac tgaatccatc aacatggtgg cacaatgcta
    37081 tgcgcgtccg cgtctgcaac cgggcgatga gattattgtc agcgtggcag aacaccacgc
    37141 taacctcgtc ccctggctga tggtcgccca acaaactgga gccaaagtgg tgaaattgcc
    37201 gcttaatgcg cagcgactgc cggatgtcga tttgttgcca gaactgatta ctccccgtag
    37261 tcggattctg gcgttgggtc agatgtcgaa cgttactggc ggttgcccgg atctggcgcg
    37321 agcgattacc tttgctcatt cagccgggat ggtggtgatg gttgatggtg ctcagggggc
    37381 ggtgcatttc cccgcagatg ttcagcaact agatatcgat ttctatgctt tctctggtca
    37441 caaactgtat ggcccgacgg gtatcggcgt gctgtatggc aaatcagaac tgctggaagc
    37501 gatgtcgccc tggctgggtg gcggcaaaat gattcacgaa gtgagttttg acggcttcac
    37561 gactcaatct gcgccgtgga aactggaagc aggaacgcca aatgtggctg gtgtcatagg
    37621 attaagcgcg gcgctggaat ggctggcaga ttacgatatc aaccaggcag aaagttggag
    37681 tcgcagcctg gcgacgctgg cggaagatgc gctggcaaaa cgtcctggct tccgctcatt
    37741 ccgctgccag gattccagcc tgctggcctt tgattttgct ggcgttcatc acagcgatat
    37801 ggtgacgctg ctggcggagt acggtattgc cctgcgggcc gggcaacatt gcgctcagcc
    37861 gctactggca gaattaggcg taaccggcac actgcgcgcc tcttttgcgc catataatac
    37921 aaagagtgat gtggatgcgc tggtgaatgc cgttgaccgc gcgctggaat tattggtgga
    37981 ttaatgacga acccgcaatt cgccggacat ccgttcggca caaccgtaac cgcagaaacg
    38041 ttacgcaata ccttcgcacc gttgtcgcaa tgggaagata aatatcgcca gttgatcatg
    38101 ctggggaaac agcttccggc tttgccagac gagttaaaag cgcaggctaa agagattgcc
    38161 ggatgcgaaa accgcgtctg gctgggatat accgtgactg aaaacggtaa aatgcacttc
    38221 tttggcgata gcgaagggcg cattgtgcgc ggcctgctgg cggtgctgtt gactgccgtt
    38281 gaggggaaaa ccgccgccga attgcaggca cagtcgccgc tggcattgtt tgatgagctg
    38341 ggattgcgtg cgcaacttag cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg
    38401 attatcgctg cgacgaagca ggtttaagca tagaaccggg tcggataaga cgttcgcgtc
    38461 tcatccgacc tgattgttta accctgacgc gccgctttcg ccatcatctt cttcagcgca
    38521 tgagaaaccg caacaaaccc aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat
    38581 gcacaatcca tccgcttcgg cccttctgcc gtggctttca tcgcacacac agtaccgtct
    38641 gactgcgggt acaccagcgc ttcagtagaa aacacgcaat cgacgccgag cttaccttta
    38701 ctgtttttca ctacgccaaa atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga
    38761 tcctgaatcg ttttcgccag atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc
    38821 gcgccaccgg tcgtgaccag cgggatttta ttgcgccgac aataagcaat cagcgccgct
    38881 ttgggccgta cactatcaat ggcatcaatc acgtacgaat aacccacgct catatactgc
    38941 gctacgttat ccggcgtcac gaaatcatcc accaccgtta cacggcactc cgggttaatc
    39001 tggcgaatac gctccgccat gacttccgct tttgccagcc cgacgttatc gcgcagagcg
    39061 tgaatttgcc gattggtatt ggtgacgcac acatcatcca tatcgataag cgtgattgcg
    39121 ccaatccccg tgcgcgccag cgcttccgcc gcccaggaac cgacaccacc gataccaacc
    39181 acacaaatat gcgcgtcagc aaacagttgc aacgcttttt caccatacag acgcgctgtg
    39241 ccaccaaaac gctgacgcca tgcatcacta attaccacag acatagcacc tcagaattaa
    39301 aaagggtgag gttatccctc accctgtcgt atccgtaaaa acggtataca gaataacaca
    39361 tcagccgcta aagacgttac ctgcgcccgg ggcggttttc agcacccaga cacgtccata
    39421 gtggttgtac caacctgcgc ggtgtccggc ttccggcccg atcccttgat agatatcgaa
    39481 gtgttggcct ttgattgcac caccgacatc cagcgccacc atcagacgca gttcgtactg
    39541 accattaaat ttgccgttat tatccagcaa cggcacttct gccagcaagg tagtacctgg
    39601 cggaataatg gaacgatcag aggcaactga cgcgcgacca accagcggca ccgcacttgc
    39661 ccctttcacc ggtgcaaaag attgcggttt aaagaagacg aaagacgggt tctgttccag
    39721 cagctcgcga acctcggctt cactgtgtgt ttcgccccag tgacgaatcg cctgcatcga
    39781 catatcttct tttttcactt cgccacggtc gatcagcacc ttaccaatgc tgcgataggc
    39841 atgaccgttt ttccctgcat agctgaaaaa gttaagcgga ctgccatcac caaagtcgat
    39901 atacccacta ccctgcacat ccataatgaa gttatccatc agggagttac tgtaagcgag
    39961 aatatattta tcactcaatg cccccgcgta gatctccgca cgagacggca gacgaccacg
    40021 ttttggcggc atacggtaaa taggatactg gaactcgccc tggcgggtat ggcgcgcctg
    40081 aattaccggc gtgtaatagc ctgtaaactg cacgttacca tagttgtcgg caccttccat
    40141 ctgccaggca tcaatgccga actggcgcat attgcgggta tcaccgcctg cgcgcagcca
    40201 ctcttgcacc gcgttataaa cattactctg gttgccatac agacgcggtg acgaattacg
    40261 gatatggtta atttgctcgg caaaatcacc ggcgttaatc ggcgcgccaa cggcatctgg
    40321 ctggttcacc agagagaaag gctgggtaaa tttcccgtct ttatattgct gtccgcgatc
    40381 ggttggtttg gaagagcagg cggcaagcat tgccacaacc gtgcccataa gaaggtactt
    40441 tacccaacgt cctttcattg ttctcttctt caggttaaaa aataaggcgc aacgaagata
    40501 acaaaccgcc gcgtttaatg aaatgagcag cctctccctg acgcaaattt tgcacaaaaa
    40561 ataggcttta gtgatttgtt tttgttcaaa atcatgccag atccgtgatc ggggtaaaaa
    40621 aaaggttgca tgaaaacgcg agcggagtat agtgcgcatc cacggaggcg gggtggagca
    40681 gcctggtagc tcgtcgggct cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa
    40741 ccaattaaaa tttgatgaag taaagcagta cggtgacgcg gggtggagca gcctggtagc
    40801 tcgtcgggct cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa ccaatcaaat
    40861 ttgatgaagt aaaagcaata cggtgacgcg gggtggagca gcctggtagc tcgtcgggct
    40921 cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa ccaattattg aacaccctaa
    40981 cgggtgtttt tttgtttcta ctctcccata aaaaaagcgc cattcagcgc ctttttatca
    41041 tccccttctc gccagcgttg ccccatcggc aaaatacgct ttaatccccg caagaataga
    41101 ctccgcaact tcctgctgga aagtcgccgt tttcagttta cgctcttcct caacgttact
    41161 gataaacgcc gtttcgacca gaatggaggg aatatctggt gcctttagta cggcaaaccc
    41221 ggcctgttca acttgatttt tatgcagctt gttgatttta ccgagcttat tcagcactgc
    41281 tttaccaaac ttaaggctgt cagcaatggt cagcgactgc accatatcga acatggtgtg
    41341 gtcgacatag cggtcaccgc ttttgctcac gccaccaatc aagtccgagg cgttctgggt
    41401 ttgtgccaga tattttgccg cagtactggt tgcacctttg gttgagagcg caaacaccga
    41461 ggaaccgctc ggctggcgac tggtaaaggc gtcggcatgg atagagacaa acaggtcagc
    41521 acgctgtttc tgggcttttg ctacgcgcac ttgcaacgga atgaagatgt cttcattgcg
    41581 cgtcatgtac accttcatat tgccctcttt ctcgatcaga gagcgcaaac ggcgagctat
    41641 ttgcaatacc acatcttttt cgcgcgtttt gtatttcccc accgcaccgg agtcttcgcc
    41701 accgtggcca gggtcaagca taatgacaat cggacgatcg cgccctgctt tacctggttg
    41761 tggaccactt tgcgctggcg gcacctgctt ttcgaggtcg cctttgttgt aatcctccag
    41821 cagcgccagc agcgggtcct gcatatcctg tgcattggcc ggatagaggt ccatcaccag
    41881 acgctcttta aacccggcga ccggcgcaag ggcaaacagc tgcggtttta cgttttgctt
    41941 taattcaaaa accatccgta cggtttgcgg gtcaaattgc ccgacgcgcg ccgacttgat
    42001 gaacgggtcg tctgcacgaa tttgtgccgc catccccttg agtaccgagt tcaggtttac
    42061 atcttcgata tccaccacca cgcgttcagg gttactcaac gcgaactgct tatatttcag
    42121 ctgacgattt gattctaccg tcacgcgggt gtagctggac gcaggccaga cgcgcaccgc
    42181 cacgacctgg ctaaccgcag ccaggctgac ctgacttacg ctcaatagcc acatagcgcc
    42241 cgcgccttgc agtaaacgac gacggctgat tgcagtgttg gatcctgaca tgcctctccc
    42301 gagcaaaaga aatctaagct gtgtaacaag taaacgacta atttgaccgg aaactttagc
    42361 gaaagacgca taatctgtca tctaataaac ggtaaacatt ctttttatat tcacggcatt
    42421 actgataaaa aagtcgctct cgcataaaat ttacacttgc accctgcgaa aaaacagaat
    42481 aaaaatacaa taatttcgaa taatcatgca aaggggtgtg ccgtggtaaa ggaacgtaaa
    42541 accgagctgg tcgagggatt ccgccattcg gttccctata tcaataccca ccggggaaaa
    42601 acgtttgtca tcatgctcgg cggtgaagcc attgagcatg agaatttctc cagtatcgtt
    42661 aatgatatcg ggttgttgca cagcctcggc atccgtctgg tggtggtcta tggcgcacgt
    42721 ccgcagatcg acgcaaatct ggctgcgcat caccacgaac cgctgtatca caagaatata
    42781 cgtgtgaccg acgccaaaac actggaactg gtgaagcagg ctgcgggaac attgcaactg
    42841 gatattactg ctcgcctgtc gatgagtctc aataacacgc cgctgcaggg cgcgcatatc
    42901 aacgtcgtca gtggcaattt tattattgcc cagccgctgg gcgtcgatga cggcgtggat
    42961 tactgccata gcgggcgtat ccggcggatt gatgaagacg cgatccgtcg tcaactggac
    43021 agcggtgcaa tagtgctaat ggggccggtc gctgtttcag tcactggcga gagctttaat
    43081 ctgacctcgg aagagattgc cactcaactg gccatcaaac tgaaagctga gaagatgatt
    43141 ggtttttgct cttcacaggg cgtcactaat gacgacggtg atattgtctc agaacttttc
    43201 cctaacgaag cgcaagcgcg ggtagaagcc caggaagaga aaggcgatta caactccggt
    43261 acggtgcgct ttttgcgtgg cgcagtgaaa gcctgccgca gcggcgtgcg tcgctgtcat
    43321 ttaatcagtt atcaggaaga tggcgcgctg ttgcaagagt tgttctcacg cgacggtatc
    43381 ggtacgcaga ttgtgatgga aagcgccgag cagattcgtc gcgcaacaat caacgatatt
    43441 ggcggtattc tggagttgat tcgcccgctg gagcagcaag gtattctggt gcgccgttct
    43501 cgcgagcagc tggagatgga aatcgacaaa ttcaccatta ttcagctcga taacacgact
    43561 attgcctgcg ccgcgctcta tccgttcccg gaagagaaga ttggggaaat ggcctgtgtg
    43621 gcagttcacc cggattaccg cagttcatca cggggcgagg ttctgctgga acgcattgcc
    43681 gctcaggcga agcagagcgg cttaagcaaa ttgtttgtgc tgaccacgcg cagtattcac
    43741 tggttccagg aacgtggatt taccccggtg gatattgatt tactgcccga gagcaaaaag
    43801 cagttgtaca actaccagcg taaatccaaa gtgttgatgg cggatttagg gtaagcgatg
    43861 aaaatcgccg gatgcggcag gcgtaacact cgtgcgtcgc atccggcaat tacgtttatc
    43921 cccgcgaact aaacaacgcc gccagaccac tgcgccgctc agtacgagtg gcgattgccg
    43981 cacttaatat acgctcatcg gcatacagcg acagacggcg acgcgcgcgg gtcaccgcgg
    44041 tgtataccag ctctcgcgtt actatcggcg tgcgttggct cggtaaaatc aacgccgcat
    44101 ggtcgaactc cgatccctgc gatttatgta ccgtcatcgc ccacgtagtt tcgtgctctg
    44161 gcaagcgact cggttgcaca gacttaatat tgccgtccgg catcgcaaac cagacgcgcg
    44221 tcccctgccc gcgatccagc gcaataccga tatcgccatt aaacaaccca agcgcgctgt
    44281 cattacgggc aatcatcacc ggtcgacctt cgtaccaacg agagtgcgaa tgacgatgaa
    44341 ttttgcgctt ctgttgcata aactgctcaa ttcgctcatt cagtccagcc acgccaaatg
    44401 gcccttcccg cagggcgcac aaaagttggt actcattgaa cgcctgaatg attaaatccg
    44461 gctcggcacg cgcttgcagc agatccagat aacgtccata acccgcaaga gcttcctcaa
    44521 gcatcgcaat ataatcttcg ccgctctgta aaagccgttt ttcaatatca gtaaaatcct
    44581 gctgaaaaac ggttttcact gccgttttat caccacggtt gatcgccgca gctaactgac
    44641 caatgccaga atcgctgccg aaacgatagc ttttttgcag caggcagaga ctgtcgcgca
    44701 aagatgccgc ttctgtgccg gttcctgccg gaacgtgagt tcccgtcagg cggcttagct
    44761 gcccggcacg ctcggcggta aagccggcat tggcataagc gcagatatcg cccagcacag
    44821 ccccagcctc aaccgaggcc agttgatcac gatcgccgag aaagatcact cgcgcatgat
    44881 cgggcaaggc gtcgatcagt ctcgacatca taggcagatc gatcattgac gcttcatcta
    44941 ccaccagcac atcaagatgc agcgggttac cggcatgatg acgtaaacgc tggctacccg
    45001 gctgcgcgcc cagcaatcgg tgcaaagtgc tggcatcttc cggaatgcgt ctcttttgtt
    45061 catcggtcag cggtaactgt cgcaaagcct tgccgagaga ttcggttaag cgcgcggcag
    45121 ctttacccgt tggtgcagcc agacggatac ggcagcgttc gccgtcggcc atttgaatta
    45181 acgctgccag caacttcgcc acggtggtcg ttttaccggt gcctgggccg ccggaaatca
    45241 ccgagatccg ccgcgtcagc gccactgctg ccgccacttt ttgccagtta atttcatcgc
    45301 ttactggaaa aagtttgtcc agggtttgcg ccagtagagc ttcatcaacc tcaatggcat
    45361 gattcacctc attgaagaag cgcgctaccg tccgctcgtt acaccacatg cgattcaaat
    45421 aaagacgatc gccacagagg atcatcggcg tgggttcatc tcccctgctg accgcttgag
    45481 aagccagcaa ggattcttcc caattttgta gctcaccgat ttcactgaca caggtcgcca
    45541 acagcggatg cgacgcctcg ttattttcca gtcgtgaaag cggcaaacag acgtgtccct
    45601 ctccggcatc atgacttaac agtgccgccg cgagggtgac ggcaggatgt tcatctcccg
    45661 ccacggtcag ggcaaattgc acatccagcg ggcgtagctg tttgtgctcc acagcttcca
    45721 gtaattgctt ttgcaatttc attacgcctc ctccagggtc ataccggcaa acatctcatc
    45781 catcagggca atcaacccgg cgttgggtcg ggttgtgtaa atcccttgtt gcggatgttc
    45841 tttatcaacg ccacgcagga acagatagat aacgccgcca aagtgacgat catagtcgta
    45901 atcagcaatg cgatggcgca gatagcgatg cagcgccagg gtataaagct gatattgcag
    45961 atcatagcgg tgtgcctgca tcgctgctgc catagcctgt tgggtgtaag ccgaactgtc
    46021 ttcacccagc cagttggatt tatagtcgag caggtaataa cgcccttcgt ggcggaatac
    46081 caggtcgata aagcctttta acatgccgcg tacctgcatg aactccagcg gcgggcagcc
    46141 agcggatagc gggtcaaact ggcgaattag tgcatcaagc tggctggcga tgagcggttc
    46201 gctaatcggc aggtaaaact ccatctccac ctgtttatcg cggtcggaaa gctgactcag
    46261 gctaacaccc gtttcattga gaggtgcctg gaggacagcc gtgatccact cggtcaacac
    46321 cggttcccac tgcgattcaa agccgccgag ttccagtttt tcctgtaccc agttcgggtc
    46381 aaccggctgg gtaaaatcga ggtcttcaaa caaactgtgc aagaatgtcc ccggtgacgc
    46441 accgcgcggg aactgatgcg gtgttaacgt cggttcttca acgacgctga ccacgcctgc
    46501 ggcatcaaca tccagccgtg gcatcagatc ctgagcgata ccgtgaccac gctgctgcaa
    46561 gccggagtag ctggtgacac gccagttatc gccgggcaat cgttgtagtg tcctcgcatt
    46621 cagttctgca gttaaagcat cattaacctg ccagggctgg ttatcaccag tttgtgccgt
    46681 ttgccaggca atatcatcat cgcataacgc ttcaatacag gtgcgaagcc ctgccgcatc
    46741 ttgcggttcc cctttttgca gcaaacgccc gagcgcactt tggtggacgt cggtgtcacc
    46801 ttttttatcg ccacgacggc gcaccagcgg tgcaacgccg agactgcaat gccaaaccga
    46861 acgcgtcagc gccacgtaaa gcaaacgcag atcttccgcc agacgttcga cctccgcgag
    46921 gtcgacgctt tctggcgcag cattaagatc caaaactgcc tcaaacgagt ggcgatcgtg
    46981 ataaaacgcc tgatcctgga cgcggaaatt ggtaataaat ggcaaccaga ccagcggata
    47041 ttccagccct ttcgatttat ggatcgtgac aatctgcacc agatgtttat cactttcgag
    47101 gcgcatttgt tggctggagg cattactgtc tggctcgagg atatgttgcg ataaccagcg
    47161 taccagcgca tgttcacttt ccagctgcgt tccggcttct tgaagcagtt cgctgatatg
    47221 caagatatcg gtaagacgcc gctcaccgcc tgccgttgcc agcaagtttt ctgcaatgtt
    47281 acgcgccgac atcagcgccc gcagcatcgg cataacgcca cgtttgcgcc agatttgccg
    47341 ataaccatcg aactcttcga ctaccgcatc ccacgcatgt tcgtcattat tcagcgtttc
    47401 aatatccagc gcattcagcc ccatcattga cgttgccagc gcactgcgca gggtgttctc
    47461 acgttcgggc gtcatcaccg cctgcaacag ccaaagcatt tcctgcgctt ccagagtttc
    47521 aaaaacacta tcgcggttcg aaaggtaaac ggaagggatt tccagcaacg ttaaggcatc
    47581 gcgcacctgg gcggcctcct ggcggctgcg caccagcacg ctgatgtccg aagcacgcac
    47641 cggacgcgcg tcgtcgccgt tcatcagcaa cgcttcgccc cgctgtccgg cttgtagcca
    47701 gtcgcggatt tgcgcagcac atacctgcgc catggtactt tgatagtcgc caacgccgca
    47761 gctttcgcct tccatcagcc acattttcat cgcaggctgc gtttcacctt taaagacaaa
    47821 acgtaacgcc tgatttttcc cggctgattt cactggaata aacggtattt cgcgaaacat
    47881 gaacgcgtca tcagtctggc tgaaaagctt gttcacactg ttcaccattc caggtgcgga
    47941 gcgccagttg gtgtctaaag tgtagtgggc gtgaacttca ctacgcgcct tcatataagt
    48001 gaagatatcc gcaccccgga atgcatatat ggcctgcttc gggtcgccaa tcagcaacaa
    48061 tgcggtttct ggctgatggt gccagatacg gcgaaaaatt cggtactgct gggggtcggt
    48121 atcctgaaat tcatcgatca ttgccaccgg gaatcgcgta cggatcgccg ctgccaacac
    48181 ctcgccgctt tcgctacgca gcgcggaatc gagccgactt aacatgtcat caaaacccaa
    48241 ttcgccacgg cggcgttttt cacgcgctac tgtttcgcgg atctcagcca atgcgcgggt
    48301 gatcaccaga tcacggatcg acaatggttc tgcaagcagt tgatcgatcg cctcaaacag
    48361 tggatgtcgc ggggtttccc ccccggcttt cgtgcgatct tctaagaaac gctgggagaa
    48421 tttttccagc gactccggca actgataact gtttgtctct tcttctgccc aggcgctgat
    48481 cttgtcgatc catttagcct gattgctacg gttaaacttg cgtcgatcaa taccagaaga
    48541 ttcgatcagc gcatccagtt cacccactgc gtcgcgccac tgctgtttta ccgtatcaat
    48601 acgcgccaca atttgcgcgt gacgggaagc tagcgtttca tcatcgggcg gcggtgcttt
    48661 gataaccggc gcttcgcctt gcagataacg attaatatcg cgcagcaacg cctgcggccc
    48721 tttccaggtt tcaaagacga cctgggctat ttcacgcggc agcgggtagc agtggcgacg
    48781 ccagaaatcg gcgcaggcct ggtagcgtag cagagactca tcttcaatca gctgctgctc
    48841 aaacagcatg ccggattcaa aggcattcag gttgagcatg cgctggcaaa agccgtgaat
    48901 agtaaagact gccgcttcat ccatctgccg ttcggctaac aacaaccact gcgcggcttg
    48961 cgctttatcg tcgacctctt ccagcaggtg ttcgtacaat ggattgtcgg tggtttcacg
    49021 cagacaggcg atgcgcaact cgtggatatt gctacggata cgaccgcgca attctgccgt
    49081 ggcagcctcg gtaaaggtga ccaccagcag ttcttcaacg gtcagcgggc ggggaaaggc
    49141 ggcggaaccg cctagtccaa gtaacaggcg caaataaagc gccgcaatcg taaaggtttt
    49201 gcctgtgccg gcagaggctt caatcaggcg ctcgccctgt aagggcaagc gcaaaggatc
    49261 tagtgtctcg gcgacatcac tcattctttt cactcatcag gggcattgtt tgctgcaacg
    49321 cgctgacgtt ctcccacact ttccagcctt caggatgcac atattcggct ttcccgtttt
    49381 ggctgccgga aatctgcgac aggatagcca tgccttgcgg ctcgactacc gcctgatgga
    49441 agaaatcggc cagtttttgc ggcgtcagca gtttaatctg ggccacgatt ttatcacgcg
    49501 aatcgaagcg catattgccg cgatcgaaat ctttacttaa cttcgatgct tcttcgccga
    49561 gcgtttgcgg tgcctgcagc atctgggtaa ttaccgcctg ctggatttgc gcaaactcat
    49621 ctggcttcat cgctcgcaat tttgcctctg cggttgggaa aaacgccttg taacgctccc
    49681 acaagaatga aggctgttta tcattgcttt gcaaaaggaa gcccatgccc cactgacgcc
    49741 ccacgctcat tggaaacgca aacacggcat agcccaattg ttcttcggta cgcaactgat
    49801 tgtagaacca cggctgtacg atctgcccca acagagagct ataggctgag ctggtgtatt
    49861 catcgtagcc agtcggtaca aataccgctg ccagtgcgga gtcggtgctg ttaccggctt
    49921 tttcaaagat gacggattgt tttttatcga ccactacatc tttgtttcga caccactctg
    49981 aaccatcagc gcccaactgt ttttgcacat cgcgtgccag cgttgttgcc tgggcctcgg
    50041 tcatgttgcc gataaccata aactctggtc gagcccctga ttttaaggcg tcgcgataag
    50101 ccagcacctc tttcaacgta atggagggca aaattttacg ccgttcatct cgcgagaagt
    50161 acggcacttg cgagagcatc tgcgcgggca taatcgcctg ctcaaacgct ttaccctttt
    50221 ctgcggaatc catcatctgg ttataccagg acttcgcctg ctcaagctga tcttccgtag
    50281 cggtatagct aaagtacccc tcgagcaatg cctggaacag ctgcggcaga cgctgggtgt
    50341 aaccattagc attaaccata aggccgttgt tagcgttggt ggaaaaactt atgccaccaa
    50401 ccgacgcctg gttgcttaac tgatcaagcg ccagccctgc gagataatca ttgagcgcaa
    50461 acatcacctg attgcgggcg ctgtccatgg ctttcggatt acgcaaaatc aggctgacat
    50521 cagctttggg ttcgctggaa aaataacggc ttggcgcata caccacgcgc agattcgact
    50581 catcaacaat cagctctgga tggtcatatt tcttctctga cttaatcagc gagaaatcgt
    50641 caggaatata ggggttaagc tccggtaagg agagcgcaat attggcagct ttttgctgcc
    50701 agtcagcgaa agtttgttcg ctgattttat cgacctgata cggcgcatcg acaaaataag
    50761 ccgttttatt gtgcggctct ttcgggctga tataccagat acgcgcattc tgcggcgtca
    50821 tcatcgccag acgttctttt actgctttag catcgtaccg atcggcaata ttgactgcat
    50881 ccagcgtatg ctcaaccgga acgcgaatca tggtatctgc cagccattcg acgtaatcca
    50941 tatcacgggt gatcgacgga taacggaagt cgatatccag cacattcgcc agttcatcga
    51001 agtattgttt atcaatgcct ttttcacgta acagattgag atagctaaaa attgccgcca
    51061 caacctgatc gcgattagcc aggcctttat cggttaaaga cgcagagatc gctaatacgc
    51121 cgctgttgcc gttgacgata ggatcggagt tggcgctaat gccctcaact aatccctgct
    51181 tttgcagcca gtcggaaagt gtacctgggc tgcgattacc aatcagatag gtaatcaatt
    51241 catccgtttt actacggaac ttcgctgagt tgttatcgat gcgaaactca acgcgcagaa
    51301 ctttacgcgg cattgccggg acgtaatgaa tgataatgcc cttttgcgcg tcggtgacta
    51361 ccggcacggt gatttccggt tttttgctct ctttgttcgg cacgcgacca aaggtgtccg
    51421 ccgccatttt tgccaactcc ggcagcggtt tattgctgta aataaccgcc ttcatcagat
    51481 tggcggaata gtacttctcg tggaaatctt tcagcgcctg ctgcaccgga ttaccaggtt
    51541 tatcgcttaa agtttcgagg ttaccaccag aaaactttga accggggtgt gccgggttaa
    51601 tggtttctgc gctgacctgt gccatgcgca tcccgtcacg cgtacgcgcc atggttaatt
    51661 cagcgttcac cgcattacgc tcacgttcgg catatttctt gtcgagcaaa ggttcagcaa
    51721 tagcatcggc caggcggtct accgcaccag gcaaggcgtc gttctcaact tccagataga
    51781 aagccgtgcg atacggcgca gtgctggcat tgtgactacc gccgtgcatt ttgagatatt
    51841 cggccagact gtcagcctgc gggtactttt tcgaccccat cagactcata tgttcaaggt
    51901 aatgtgccag cccctggtac gcctcgggat cttccagcga cccaacgggc accaccagcg
    51961 ccgagagcga tttaactgcc tgcggatcag aaaccagcaa gaccaccata ccgttatcca
    52021 gacgtatagc ctgatactgg cggttatctt tatcactttt acggatggtt tcctgaatcg
    52081 gctgccatcc cgtttctgcc tgacttaagg gtgcccaaag ggcaactaac aacaataatg
    52141 ctttgaacca ggtgctgcgg ggcattctcg gacctcataa tcaacttaat tttctgtcca
    52201 gattcaacac gttaacgcaa atcatctgcc agaatttaat cttgtgctgc acgagtcagc
    52261 ctatgtttat ataaccatca gtccgtgact ggtgcgcatc ataaagtaag cggatagatt
    52321 gcgcaatttt tatacagcac tcatgactga ttaaagcgaa acagcggtaa caggaaacgt
    52381 tgcgactgtt caacgatggc ctccattgtc tctggtgtta attgccgcca gagcctttga
    52441 taccagatat catcaccttc gccacgcacc atcatgttac cttcgtaagc ctgaaggaat
    52501 ttcgtacggg ctttttgcaa cgtggaatcg tcatccagca tggcatcgtt ttgcgcgtca
    52561 taacaggttt ttagccacgc gccgccactt tcaggtaaca ccagcaatgg cgcggacatc
    52621 ccttcacgat acccctcaat cagttgtgag aggtaatgca aagcctgttc ggctgcaagc
    52681 ggcggaaaac gccactcgcc gtctttgcgt agaaaaaggc gactttcacc attaccaccg
    52741 ctggcacagt agacaaggtg ttccagccaa agttgcattc cctgcgccac acttaataaa
    52801 gagggacgcc agcgcaacag gccatccggc tgcacctgcg gcaaccagcc agttatctgc
    52861 acaccgttgc aggcgagatc aatttccata ctctgccccg gctggcgaca ggcaatgact
    52921 ctgtcggcaa gctgctgcat ctcctggcac tgagtttccc agaaaatttc accaaaagcg
    52981 ccatacggta aatcccctgc cgcccggaag cgacggaaca agcgttcggc atcatcctgc
    53041 tcaaccagtg cattcaataa ctgctgattg atttgataac ggctaagccc ttccagaata
    53101 aatggctcgg tgtcggggat ttcgctgtct tcagtacgga agttcacctg caaacgcatc
    53161 tggaagaacg cccgcaccgg atgtgcccag aatcgttgta gcgtttccag cggcacggtt
    53221 tccggtaagg taaacggcag cggctgaaca aattcagaat gtgctttacc agcctggctg
    53281 gccgcaggta gccattcacg agcatagctt tgtcgttcgc ctggctgata gttttgtgga
    53341 tcaaacggca ttcgggtatg gaggcaagta agatgcgctt ttacccttgc ctcgctttca
    53401 tcacagttga gcgcttcatc gcccggtaga taatgacttt gcccgatgta gtcgatcagt
    53461 tcctgcacca gtaccgacgg gaaacgttca ctgttatcct gaatggaacg cccgatatag
    53521 ctgatataga gtttttgctg cgcggaaatt aacgcttcca ggaacagata gcggtcgtca
    53581 tcgcgacggc tacggtcgcc acgcttcggt ttctggctca tcaggtcaaa gcccaatggc
    53641 gcaagctgac gtggataaac gccgtcgttc attcccagca ggcaaaccac tttgaacgga
    53701 attgaacgca ttggcatcag agtacaaatg ttaaccggtc cggcgagaaa acgctggctg
    53761 atacgttctt gatccagacg ctgtgccagt tcatcacgca atagtgacag cggcaccgcg
    53821 tcgccatact gcgcacctaa accttcggcg ataatcgcct gccattgttg ttcgatcagc
    53881 gtcatcgccg cttcggtttc cgcatccggc aggaagaagg cgctgagcat atcgcgacaa
    53941 accggcaacc actcttctag cggacgctcc tgtgccagcc cgcgacgcca gatgtttagc
    54001 tgcattagca gtgaagccag atgccccacc agttctgcaa ttaagccgct cgattcatca
    54061 taaggtagaa ccgattgcca ctcgccctgc gcgctctcca tcgcgtagcc cagcaacata
    54121 cgcgtcaggc caaatcgcca ggtgtgttgt ccggtggcgg ggagttccag ctcgcgaacg
    54181 ttgtcgtcat ctatccccca acgaatgccg gattcgttga cccactggcg taaataacgc
    54241 agcccttctt cggtgatgtc aaaccgcgcc gccagcaccg gcacatccag taatgccagc
    54301 acgtcttccg acacaaagcg gctgtctggc aacgataaca ggctgataaa cgcttccagt
    54361 accggatgcg actgccgcgc ccgacggtcg gaaatggcgt aaggcaggta acgatccgca
    54421 ggtgcgctac caaacacagc ccgaataaac ggactgtagc tgtcgatatc agccaccatc
    54481 acgatgatgt cgcgcggagt aagtgtcggg tcttcttcca gcatcgccag caggcgatcg
    54541 tgcaaaactt caacttcacg ctgcgggcta tggcaaacgt ggaaggtgat actgctatcc
    54601 agtggatcaa gcgggcgttt gttatcgcta cgggaaaact cttcgatgtt cacaccagca
    54661 acggcacggt tttccagttc cagaatgtca gactgaatgt tatgcagcag gttatctggc
    54721 gtgacatcga caaaggcgtc cagctcctgg ctgctctcca ggtcagaaag gagataaatg
    54781 tagtcgcgcc caagcttacc ccatgaagcc agcagcgggt tgccgacatc ctgttcacca
    54841 tcgctgttaa agagctgccc ggcattttcg ctatcacgaa atagcggcaa ttcgcgatct
    54901 tcaaaactgt gtcgacgctg acgggtcagc agtttcgcca gataagcagg atctttaata
    54961 tcgccccagt aataacggca ggggttggta aacaggagat ggatttcaat atgtttaccc
    55021 agcgcctgta gcgcctggag ataaacaggc ggtaacgcgg aaataccgca tataaagacg
    55081 cgcgaaggta accccggcgg gcaggtcgtc gcggactcca gcgtttcgat aaagcgctga
    55141 tagagattgg cgcggtgcca gcgcggttgc ccgagttgat gggtatattc caccagcgcc
    55201 ttccacaacg gggcttgcca ggcctgtgct tctcccagcc cttcaaccaa atgtcctgtt
    55261 tcccactgtg ccagccagtc cggacgatag accagatact ggtcaaacag gtccgccgct
    55321 tttgaggaaa gctggaacag ttttcgcttg tcgctatcgt cagtcagata atgccgcaac
    55381 agggtaaagt cttcgcgctc cagcaattgc ggcagcagag tcatcagttt ccagctcatg
    55441 ctctgtttgt taaaggcgct ctctttgggg atttccggta acacccggac gaacatatcc
    55501 cagataaagc tcgctggcag cggaaaatca atgtttgccg caatactaaa cttttgcgac
    55561 agggtcattt gcagccactg cgccataccg gtactttgca ccagaatcat ctctggttcg
    55621 aaaggatcgt ccagccgttc gcgttcgaca ataaactcca tcaacgcttc cagcacgtcc
    55681 agacgattgg aatggtagac ccttaacata gcggctcctg actactgacg attcgggcaa
    55741 tgcaggcggg tcatctcgcc ttctctgccc ccgggtgaaa ctagcgtaac gctgatgctg
    55801 acacatcccg cctgcgatgt ctgcattcgg ttgacctgcc agttggcagg tggcgaaatc
    55861 gcgcgcagtt gcgtttgctg ccaaccatgc cgccagagct gttggtactg gtttctgctg
    55921 gcgagactgt tcattaatgt tcgctggata cccgataacg cagtgacaat catcaccatc
    55981 agcaccatcg caaccattac ctccggcagg ctaaagcctt gttgattctt cagggaagct
    56041 gacataacgc cctctctttc agcggacaaa aatcgctcca gccgcgtggc gaaaaaacaa
    56101 tgtttccatc aatgacttcg cctgttcgcc acaacgaaac gccttcataa ccggcaacca
    56161 ataaggcttc attatctgcc agtaaacgca aacaaacccg ggcatcggtt ccagcgtact
    56221 gcgagcactg aactgcgggc tgcgtctgcc aggagtgcat tttcccccac gccagcgccg
    56281 actgaacgat ggcctggcgg cgcaatgact gactttctat gctcacgcga gaggcaaaac
    56341 tgcgatcctg ctgactcatt ccttgcaata gcaagctacc caaaaccagc agcatcagga
    56401 ccagagccag tgacgaaaca cctttttcgc ggttcacagg ttgaatcctg tcacgctata
    56461 gctggcatcc accacggttt gcggttcaga cttactggca gcacgcatat taaccgtcaa
    56521 caccggcgag aagccgctga catcctgacg tacgacctga aaagtgtcga taatgatggc
    56581 atccggatta gtgactttat cccagccctt accttcacag gatgtcgcac cgcgtagcgt
    56641 ttccagcaca tgctccttca gacgaaatcc aatctggtca gactctttta ccggttcgcg
    56701 atcccagata ccgttactgt tcgcatccca ctgcacaatg atacagtcac cctgtccgac
    56761 aatttccagc ccttcgccgg tacagctgcc atgacaataa cccgccctct ggagatgctt
    56821 cgcgacggta aatacccgca gccagatttc atcttccagc gccagcttac gggtgctcgt
    56881 taaactttca cgctgtaacg caggcagaaa gcgtgcagcc cccagcaaca atacgctact
    56941 gatcgccata gcaatcaaca cttccagcag agaaaaacct tgctctttta caggcatcct
    57001 tctgtttctc cttgctgaca aagccggagt cttccccacg gcgaaaccac cagccaccac
    57061 tcgcccgttg agtttttgaa gcgaatatgc ccggcccatg cggtattgcg caggccaaag
    57121 aaagcaagcg aaggtgtcag gtcgctcatt tcgacttcgg gccagcgtgg cacaaagacc
    57181 aatggtgaac tgccatgaca ggtattggcc ccagcagcgg aactcacaag gcaccataac
    57241 gtcccctccc tgataacgct gatactgtgg tcgcggttat gccagttggc atcttcacgt
    57301 aaatagagca aatagtcccg cgcctggctg gcggtttgcc atagccgttg cgactgctgc
    57361 cagtattgcc agccatagag tccacttgcg cttagcatga ccaaaatcag catcgcgacc
    57421 agcgtttcaa tcagcgtata accacgttgt gttttcatgc cggcagtatg gagcgaggag
    57481 aaaaaaagac gagggccagt ttctatttct tcggcgcatc ttccggacta tttacgccgt
    57541 tgcaggacgt tgcaaaattt cgggaaggcg tctcgaagaa tttaacggag ggtaaaaaaa
    57601 accgacgcac actggcgtcg gctctggcag gatgtttcgt aattagatag ccaccggcgc
    57661 tttaatgccc ggatgtggat cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa
    57721 gatggattcg ggtttacgtt tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa
    57781 ttgcagatga gtttgatcca tatgattgct gtacaggtgc gtatcgccac cggtccagac
    57841 aaaatcaccc acttccagat cgcactgctg cgccatcata tgcaccagta acgcgtagct
    57901 ggcaatgttg aacggcaggc cgaggaagac gtcacaggag cgctgataaa gctggcaaga
    57961 gagtttgccg tctgccacat agaactggaa gaatgcatgg cacggtgcca gcgccatttt
    58021 atccagttcg cctacgttcc acgctgaaac aataatgcgg cgggaatccg ggtcgttttt
    58081 cagctggttc agtaccgtag tgatctggtc aatatgacga ccatctggcg ttggccaggc
    58141 gcgccactgt ttaccataca ctggtccgag gtcgccgttt tcatcggccc attcgtccca
    58201 gatggtgaca ttgttttcgt gtagataagc aatgttagtg tcgccctgca gaaaccacag
    58261 cagttcgtgg atgatggaac gcaggtggca acgtttagtt gtcaccagcg ggaatccatc
    58321 ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc gttccggttc cggtacggtc
    58381 gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt tctaaatact gtttcatggt
    58441 tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc cagaccatca tgatcacacc
    58501 cgcgacaatc atcgggatgg aaagaatttg ccccatgctg atgtactgca cccaggcacc
    58561 ggtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc
    58621 aatcaggaac aagccagaga cagctcccat tgggcgcggt ttacgaatat acaggttgag
    58681 gataataaac agcaccacac cttccagcag cagctcgtaa agctgtgatg ggtggcgcgg
    58741 cagcacaccg taagtgtcga aaatggattg ccactgcggg ttggtttgca gcagcaaaat
    58801 atcttctgta cgggagcctg ggaacagcat ggcaaacggg aagttcgggt caacgcggcc
    58861 ccacaattca ccgttaataa agttgcccag acgcccggca ccaagaccaa acggaatgag
    58921 tggtgcgata aaatcagaaa cctggaagaa ggaacgttta gtacggcggg caaagataat
    58981 catcaccacg ataacgccaa tcaggccgcc gtggaaagac atgccgccgt cccagacacg
    59041 gaacagatac agcggatcgg ccataaactg cgggaaattg tagaacagaa cataaccaat
    59101 acgtcccccg aggaagacgc cgaggaagcc cgcatagagt aagttttcaa cttcattttt
    59161 ggtccagccg ctgcccggac gattcgcccg tcgtgttgcc agccacattg caaaaatgaa
    59221 acccaccaga tacatcaggc cgtaccagtg aagcgccacg ggtcctattg agaaaatgac
    59281 cggatcaaac tccggaaaat gcagatagct actggtcatc tgtcaccaca agttcttgtt
    59341 atttcgctga aagagaacag cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg
    59401 tgcgaataat agcacaaggg gacctggctg gttgccggat accgttaaaa gatatgtata
    59461 tgatccgcgc tataaccctc cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc
    59521 tgcaacctga tggcgaactt cggtcgccag ttgcgcttcc agactacgct gcgcaagatt
    59581 ttctgcttcg gcataatcaa tgcgccgcag caggtatttt acccgcgcta cagaacgtcc
    59641 gttcatagac agatggcgat acccaagccc aatgaggatt gccacgcaca tgggatcgcc
    59701 cgccatttca ccgcacaaac ggagatcgat tccatgtatt tccgcttccc gggcgatcat
    59761 cgccagagct cgtaacattg caggatgaag actgtcataa atgttcgcca cccgggtatt
    59821 gttgcgatca acggccagaa tgtattgagt cagatcgttg gtgccaacag agatgaaatc
    59881 gacccgcttt gccagatgcg gcagcataaa taccattgac ggcacttcca gcatgatgcc
    59941 gatacgtggt ttgggaattt cgtaaccgat catctcctcg acttcacgtc cggcacgttc
    60001 aatcaggcgg cgtgcttcat caacttcatc gaggcttgtg accatcggca acagaatatt
    60061 caggttgccc gtagcggcat tagcacgcag catcgcccgc acctggatca agaagatctc
    60121 cggctgatcg agcgtaatgc gaatcccacg ccaacccagg catggattct cttcgctgat
    60181 cggcatgtaa ggcagctgct tatctgctcc gacatccagc gtacgcaagg tgacgggttt
    60241 atcattgaac atttgcagca tcccctgata ctgcgccacc tgttcttctt ccgacgggaa
    60301 accactttgc agcatgaatg ggatttcagt gcgataaaga ccgatgccat caatacggct
    60361 gcccagtttt tcttcatgtt ccgggcttaa accagcattg agcatgactt ttatacgctc
    60421 accgcttttt aactgcgcgg gtaaattgac gtcatcttcc gccagacggc taagctcaat
    60481 ctcttcacta attagccgct gatattcttg cagcagtacc ggctccggat cgaccagcaa
    60541 ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc agcaccgaag gctgaatatc
    60601 cgcgcccatc acggtaggga tccccagcgc acgtaccatg atcgcagcat gggagttggc
    60661 tgcgccatct cgcacgacaa caccgactaa gcgatcctgg ggcagctcag caagagttgt
    60721 cgctgacagt tcatctgcca ccagaatgaa acgttccggc caggcgttcg gcccttgatt
    60781 agcgtcatca agatgaaaca gcaatcgctg acccagcgca cgtaaatcgc cagcccgctc
    60841 tttgagatag ttatcgctta gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt
    60901 taccgcccac tctgccaccg agcctttatc aacctcggca aacaattcgc gacgcagccg
    60961 ggtgtccgaa agtaggtgcg agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc
    61021 ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt
    61081 cagtcgttcg cgttccagag ccggatccag cgttgatgcc tgatacacct gttccattaa
    61141 aggtaacgtg gcatcctgcc agccttcggc aatcgccaca ccaggtgctg ccggtaatgc
    61201 gcggattcgc gtctggcgat attgcccaaa caaggcagtc aactgcgact gagaaagaat
    61261 agctgccatc tgggtggcaa gcgtcaccag gaaggattct tcactttcgt catactggcg
    61321 caactctcgt tgctgtacca ccagtacacc aagcaactgg cgacgttgaa taattggtac
    61381 gcctaaaaac gcgcggaaac gttcttcttt tacggagggg atgtatttga agctggggtg
    61441 cttttgcgca tctgcaaggt ttatcggttc cgccagcctg ccaaccaggc cgacgatccc
    61501 ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat
    61561 caggtagtaa caacgtcgat catgatcggc caggtagacc gaacagacct cggtatccat
    61621 cgcaagacag atgtcggtaa ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc
    61681 taccttttcg actatttcgc gcaggcgagt gagcataatt ggcgtgactt aacctctttt
    61741 acgtcgataa gcagatgtgt tttgtggttt tggcgtattt tcctgcagtg acatcaccac
    61801 actcgcgaac tctttcatta ccctacggta gacatcacgt ttaaatgaca ccacctgtct
    61861 gaccggatac cagtaactta cccatcgcca gccgtcaaac tctggtgtac tgctggtttg
    61921 catattgatt tctgcatcgc cgctcaccag ctgcaagaga aaccattttt gtttttggcc
    61981 gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc ggtaatttgt agcgcaacca
    62041 gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa
    62101 ttcacggtac atcgcctgct ctgcggattc tccggggttg attccgcctt gcggaaattg
    62161 ccaggagtgc tgaccaaatc gccgggccca cattacctgc ccctggcgat tacaaatcac
    62221 gatacctacg tttgggcggt agccatcgtc atcaatcacc ggactacctc aaaataaagc
    62281 tttatatacg aatgattgtt tcatactcca agaagacggt aaaccactct ctgcagggca
    62341 ttacacacta ataacaattg aataactcac agttatgtgc agagttataa acagaggaag
    62401 gggtggatag ccgtttttat tcaccttttc tgtggataga gttgtgaaga actacggaat
    62461 tactacggga aaacccggag cattctgaat aagcccgata tagacatttt aaaatattca
    62521 ttattagtca atgcattaca tgtaatttga gtaatgaaaa agagtaattc gtgacccagg
    62581 tcacacctct catttacggg ttggcgaaag atcaaccaat gccgtattta tccacagaat
    62641 gtgccactaa gttaagcact gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg
    62701 taaatggaaa cagtagtgga ggtttttcac agttatccca gctttctgtg gataacatgg
    62761 tgtaagatcc tgtttatttt cagtgaccag atttggaaaa cctattgcag tgttgcgcaa
    62821 ctcgattacc ggcagcctaa aaagcaatat aaatcagaga attgaacaac acatgtggaa
    62881 aaagttacac tgcgaatatt cggcacataa ttgctgtttg ttttttaacc aaggtatcat
    62941 gacatgtccc aacctcgccc actcctctct cctcccgaaa ctgaagaaca gttgttagcg
    63001 caagcacagc aactttctgg ttatacattg ggagaactgg cggcacttgc cgggctggtt
    63061 acgccagaga atttaaaacg cgataagggc tggattggcg tgttactgga gatctggcta
    63121 ggtgccagcg cagggagtaa acctgagcaa gattttgctg ctctgggcgt ggaacttaaa
    63181 actatccctg tggatagtct tggtcgtccg ctggaaacaa cattcgtttg tgttgccccg
    63241 ttaacgggta atagcggggt gacctgggaa accagccatg tgcgccacaa gctcaaacgc
    63301 gtactgtgga taccggttga aggtgagcgc agcatcccgc tggcgcagcg tcgcgtagga
    63361 tcaccgttgc tgtggagccc gaatgaagag gaagaccgac agctacgcga agactgggaa
    63421 gaattaatgg atatgattgt tctcggtcag gttgagcgga tcaccgctcg tcacggggag
    63481 tatttacaga tacgaccgaa agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc
    63541 cggggcgaac ggattctgac gctgccacgc ggcttttatt tgaagaagaa tttcaccagt
    63601 gcactactgg cccgtcattt tctgatccag tagccatcgc tttgacctgc cgctttccgg
    63661 gcatataatt accgcttcat ttttttggca gggcttttta gatgttattt gcatggataa
    63721 ccgatcctaa cgcctggctt gcgctcggta cgctgacgct gctggagatc gttcttggga
    63781 tcgacaatat tattttcctt tctctggtgg tggcaaagct ccccacagca caacgtgctc
    63841 atgcgcgccg tctggggttg gcgggagcca tggttatgcg tctggcgctg ctggcatcaa
    63901 tcgcctgggt tacgcgcctg acaaatccgc tttttacaat attcagtcag gaaatttccg
    63961 cccgtgattt gattctgctt ctgggtggct tgttccttat ctggaaagcc agcaaggaaa
    64021 tccacgaatc cattgaaggt gaagaagaag ggctgaaaac acgcgtttca tcattcctcg
    64081 gcgctatcgt gcagattatg ctgctggata ttatcttcag cctcgactcg gtgattaccg
    64141 ctgtgggtct gtcagatcac ctgtttatta tgatggccgc cgtggtgatt gccgtaggcg
    64201 tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga acgccatcct tcggtaaaaa
    64261 tgctggcgct ctctttcctg attctggtcg gctttaccct gattctggaa agtttcgaca
    64321 tccacgtacc gaaaggttac atctacttcg cgatgttctt ctctattgcg gttgaaagcc
    64381 tcaacctgat tcgcaacaaa aagaacccgc tctgatactc cgtacgctct cctgcgggag
    64441 ggcgtactca ctcctgttac cctcacagtt taagattttc ctgctttcaa gatatataac
    64501 gtcggtttat aaacagacta ttatcatagg tgggccaaat gaggataacc ggatgaaaaa
    64561 atgggcagta ataatttctg cagtcggact ggcgtttgct gtttccgggt gttccagtga
    64621 ttacgtcatg gcgaccaaag atggccgtat gattttgacc gatggaaaac ctgaaattga
    64681 tgatgatacc gggctggtga gttatcacga tcagcaaggt aacgcgatgc aaattaaccg
    64741 tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg tcagcatccg gctgtaatgc
    64801 cgatcagtta ag
//
LOCUS       DAETQN010000007        63145 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.007, whole genome shotgun sequence.
ACCESSION   DAETQN010000007 DAETQN010000000
VERSION     DAETQN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.007"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..415)
                     /locus_tag="K0W68_000485"
                     /pseudo
     CDS             complement(1..415)
                     /locus_tag="K0W68_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(521..3082)
                     /gene="mutS"
                     /locus_tag="K0W68_000486"
     CDS             complement(521..3082)
                     /gene="mutS"
                     /locus_tag="K0W68_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBI3072154.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            3158..3292
                     /locus_tag="K0W68_000487"
     CDS             3158..3292
                     /locus_tag="K0W68_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072155.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            3369..3722
                     /locus_tag="K0W68_000488"
     CDS             3369..3722
                     /locus_tag="K0W68_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBI3072156.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(3759..5836)
                     /gene="flhA"
                     /locus_tag="K0W68_000489"
                     /pseudo
     CDS             complement(3759..5836)
                     /gene="flhA"
                     /locus_tag="K0W68_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(5910..6920)
                     /gene="hypE"
                     /locus_tag="K0W68_000490"
     CDS             complement(5910..6920)
                     /gene="hypE"
                     /locus_tag="K0W68_000490"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBI3072157.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(6917..8038)
                     /gene="hypD"
                     /locus_tag="K0W68_000491"
     CDS             complement(6917..8038)
                     /gene="hypD"
                     /locus_tag="K0W68_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBI3072158.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(8038..8310)
                     /gene="hypC"
                     /locus_tag="K0W68_000492"
     CDS             complement(8038..8310)
                     /gene="hypC"
                     /locus_tag="K0W68_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBI3072159.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(8301..9173)
                     /gene="hypB"
                     /locus_tag="K0W68_000493"
     CDS             complement(8301..9173)
                     /gene="hypB"
                     /locus_tag="K0W68_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBI3072160.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(9177..9527)
                     /gene="hypA"
                     /locus_tag="K0W68_000494"
     CDS             complement(9177..9527)
                     /gene="hypA"
                     /locus_tag="K0W68_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBI3072161.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            9739..10200
                     /gene="hycA"
                     /locus_tag="K0W68_000495"
     CDS             9739..10200
                     /gene="hycA"
                     /locus_tag="K0W68_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBI3072162.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            10325..10936
                     /gene="hycB"
                     /locus_tag="K0W68_000496"
     CDS             10325..10936
                     /gene="hycB"
                     /locus_tag="K0W68_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBI3072163.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            10933..12759
                     /gene="hycC"
                     /locus_tag="K0W68_000497"
     CDS             10933..12759
                     /gene="hycC"
                     /locus_tag="K0W68_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBI3072164.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCDYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            12762..13685
                     /gene="hycD"
                     /locus_tag="K0W68_000498"
     CDS             12762..13685
                     /gene="hycD"
                     /locus_tag="K0W68_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBI3072165.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            13703..15400
                     /gene="hycE"
                     /locus_tag="K0W68_000499"
     CDS             13703..15400
                     /gene="hycE"
                     /locus_tag="K0W68_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBI3072166.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            15410..15952
                     /gene="hycF"
                     /locus_tag="K0W68_000500"
     CDS             15410..15952
                     /gene="hycF"
                     /locus_tag="K0W68_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HBI3072167.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            15952..16719
                     /gene="hycG"
                     /locus_tag="K0W68_000501"
     CDS             15952..16719
                     /gene="hycG"
                     /locus_tag="K0W68_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HBI3072168.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            16716..17126
                     /gene="hycH"
                     /locus_tag="K0W68_000502"
     CDS             16716..17126
                     /gene="hycH"
                     /locus_tag="K0W68_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HBI3072169.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            17119..17589
                     /gene="hycI"
                     /locus_tag="K0W68_000503"
     CDS             17119..17589
                     /gene="hycI"
                     /locus_tag="K0W68_000503"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HBI3072170.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            17614..18387
                     /locus_tag="K0W68_000504"
     CDS             17614..18387
                     /locus_tag="K0W68_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072171.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            18549..18860
                     /locus_tag="K0W68_000505"
     CDS             18549..18860
                     /locus_tag="K0W68_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HBI3072172.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            18857..19276
                     /locus_tag="K0W68_000506"
     CDS             18857..19276
                     /locus_tag="K0W68_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3072173.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(19390..20814)
                     /gene="ascB"
                     /locus_tag="K0W68_000507"
     CDS             complement(19390..20814)
                     /gene="ascB"
                     /locus_tag="K0W68_000507"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HBI3072174.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(20823..22280)
                     /gene="ascF"
                     /locus_tag="K0W68_000508"
     CDS             complement(20823..22280)
                     /gene="ascF"
                     /locus_tag="K0W68_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HBI3072175.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22540..23550
                     /gene="ascG"
                     /locus_tag="K0W68_000509"
     CDS             22540..23550
                     /gene="ascG"
                     /locus_tag="K0W68_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBI3072176.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            23698..24225
                     /gene="hydN"
                     /locus_tag="K0W68_000510"
     CDS             23698..24225
                     /gene="hydN"
                     /locus_tag="K0W68_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBI3072177.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            24378..26630
                     /gene="hypF"
                     /locus_tag="K0W68_000511"
     CDS             24378..26630
                     /gene="hypF"
                     /locus_tag="K0W68_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBI3072178.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(26758..27891)
                     /gene="norW"
                     /locus_tag="K0W68_000512"
     CDS             complement(26758..27891)
                     /gene="norW"
                     /locus_tag="K0W68_000512"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBI3072179.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(27888..29327)
                     /gene="norV"
                     /locus_tag="K0W68_000513"
     CDS             complement(27888..29327)
                     /gene="norV"
                     /locus_tag="K0W68_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBI3072180.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            29514..31028
                     /gene="norR"
                     /locus_tag="K0W68_000514"
     CDS             29514..31028
                     /gene="norR"
                     /locus_tag="K0W68_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBI3072181.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            complement(31025..31990)
                     /gene="gutQ"
                     /locus_tag="K0W68_000515"
     CDS             complement(31025..31990)
                     /gene="gutQ"
                     /locus_tag="K0W68_000515"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBI3072182.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31983..32756)
                     /gene="srlR"
                     /locus_tag="K0W68_000516"
                     /gene_synonym="gutR"
     CDS             complement(31983..32756)
                     /gene="srlR"
                     /locus_tag="K0W68_000516"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBI3072183.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(32823..33182)
                     /gene="gutM"
                     /locus_tag="K0W68_000517"
     CDS             complement(32823..33182)
                     /gene="gutM"
                     /locus_tag="K0W68_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBI3072184.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            complement(33288..34056)
                     /gene="srlD"
                     /locus_tag="K0W68_000518"
                     /pseudo
     CDS             complement(33288..34056)
                     /gene="srlD"
                     /locus_tag="K0W68_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            complement(34060..34431)
                     /gene="srlB"
                     /locus_tag="K0W68_000519"
                     /gene_synonym="gutB"
     CDS             complement(34060..34431)
                     /gene="srlB"
                     /locus_tag="K0W68_000519"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBI3072185.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(34442..35401)
                     /gene="srlE"
                     /locus_tag="K0W68_000520"
                     /gene_synonym="gutE"
     CDS             complement(34442..35401)
                     /gene="srlE"
                     /locus_tag="K0W68_000520"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBI3072186.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(35398..35961)
                     /gene="srlA"
                     /locus_tag="K0W68_000521"
                     /gene_synonym="gutA"
     CDS             complement(35398..35961)
                     /gene="srlA"
                     /locus_tag="K0W68_000521"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBI3072187.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFLHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            36217..37302
                     /gene="mltB"
                     /locus_tag="K0W68_000522"
     CDS             36217..37302
                     /gene="mltB"
                     /locus_tag="K0W68_000522"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBI3072188.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            37447..37944
                     /gene="pncC"
                     /locus_tag="K0W68_000523"
     CDS             37447..37944
                     /gene="pncC"
                     /locus_tag="K0W68_000523"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBI3072189.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            38024..39085
                     /gene="recA"
                     /locus_tag="K0W68_000524"
     CDS             38024..39085
                     /gene="recA"
                     /locus_tag="K0W68_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBI3072190.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            39313..39813
                     /gene="recX"
                     /locus_tag="K0W68_000525"
     CDS             39313..39813
                     /gene="recX"
                     /locus_tag="K0W68_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBI3072191.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            39941..42571
                     /gene="alaS"
                     /locus_tag="K0W68_000526"
     CDS             39941..42571
                     /gene="alaS"
                     /locus_tag="K0W68_000526"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBI3072192.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            42806..42991
                     /gene="csrA"
                     /locus_tag="K0W68_000527"
     CDS             42806..42991
                     /gene="csrA"
                     /locus_tag="K0W68_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBI3072193.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            43307..43399
                     /locus_tag="K0W68_000528"
     tRNA            43307..43399
                     /locus_tag="K0W68_000528"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43341..43343,aa:Ser,seq:gct)
     gene            43403..43479
                     /locus_tag="K0W68_000529"
     tRNA            43403..43479
                     /locus_tag="K0W68_000529"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43437..43439,aa:Arg,seq:acg)
     gene            43543..43619
                     /locus_tag="K0W68_000530"
     tRNA            43543..43619
                     /locus_tag="K0W68_000530"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43577..43579,aa:Arg,seq:acg)
     gene            43683..43759
                     /locus_tag="K0W68_000531"
     tRNA            43683..43759
                     /locus_tag="K0W68_000531"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43717..43719,aa:Arg,seq:acg)
     gene            43958..44034
                     /locus_tag="K0W68_000532"
     tRNA            43958..44034
                     /locus_tag="K0W68_000532"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43992..43994,aa:Arg,seq:acg)
     gene            44315..44881
                     /gene="yqaB"
                     /locus_tag="K0W68_000533"
     CDS             44315..44881
                     /gene="yqaB"
                     /locus_tag="K0W68_000533"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBI3072194.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            44878..45306
                     /locus_tag="K0W68_000534"
     CDS             44878..45306
                     /locus_tag="K0W68_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3072195.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            45379..46935
                     /gene="gshA"
                     /locus_tag="K0W68_000535"
     CDS             45379..46935
                     /gene="gshA"
                     /locus_tag="K0W68_000535"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBI3072196.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            47085..47600
                     /gene="luxS"
                     /locus_tag="K0W68_000536"
     CDS             47085..47600
                     /gene="luxS"
                     /locus_tag="K0W68_000536"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBI3072197.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(47664..49202)
                     /gene="emrB"
                     /locus_tag="K0W68_000537"
     CDS             complement(47664..49202)
                     /gene="emrB"
                     /locus_tag="K0W68_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBI3072198.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(49219..50391)
                     /gene="emrA"
                     /locus_tag="K0W68_000538"
     CDS             complement(49219..50391)
                     /gene="emrA"
                     /locus_tag="K0W68_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HBI3072199.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(50518..51048)
                     /gene="emrR"
                     /locus_tag="K0W68_000539"
     CDS             complement(50518..51048)
                     /gene="emrR"
                     /locus_tag="K0W68_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBI3072200.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(51139..51474)
                     /gene="ygaH"
                     /locus_tag="K0W68_000540"
     CDS             complement(51139..51474)
                     /gene="ygaH"
                     /locus_tag="K0W68_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBI3072201.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(51464..52201)
                     /gene="ygaZ"
                     /locus_tag="K0W68_000541"
     CDS             complement(51464..52201)
                     /gene="ygaZ"
                     /locus_tag="K0W68_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBI3072202.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(52325..53509)
                     /locus_tag="K0W68_000542"
     CDS             complement(52325..53509)
                     /locus_tag="K0W68_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3072203.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(53800..54792)
                     /gene="proX"
                     /locus_tag="K0W68_000543"
     CDS             complement(53800..54792)
                     /gene="proX"
                     /locus_tag="K0W68_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBI3072204.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(54850..55914)
                     /gene="proW"
                     /locus_tag="K0W68_000544"
     CDS             complement(54850..55914)
                     /gene="proW"
                     /locus_tag="K0W68_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBI3072205.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(55907..57109)
                     /gene="proV"
                     /locus_tag="K0W68_000545"
     CDS             complement(55907..57109)
                     /gene="proV"
                     /locus_tag="K0W68_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBI3072206.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(57465..58424)
                     /gene="nrdF"
                     /locus_tag="K0W68_000546"
     CDS             complement(57465..58424)
                     /gene="nrdF"
                     /locus_tag="K0W68_000546"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBI3072207.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(58434..60578)
                     /gene="nrdE"
                     /locus_tag="K0W68_000547"
     CDS             complement(58434..60578)
                     /gene="nrdE"
                     /locus_tag="K0W68_000547"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBI3072208.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTNEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(60551..60961)
                     /gene="nrdI"
                     /locus_tag="K0W68_000548"
                     /pseudo
     CDS             complement(60551..60961)
                     /gene="nrdI"
                     /locus_tag="K0W68_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(60958..61203)
                     /gene="nrdH"
                     /locus_tag="K0W68_000549"
     CDS             complement(60958..61203)
                     /gene="nrdH"
                     /locus_tag="K0W68_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBI3072209.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(61451..61780)
                     /locus_tag="K0W68_000550"
     CDS             complement(61451..61780)
                     /locus_tag="K0W68_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBI3072210.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            61932..62276
                     /locus_tag="K0W68_000551"
     CDS             61932..62276
                     /locus_tag="K0W68_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBI3072211.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(62313..62762)
                     /gene="alaE"
                     /locus_tag="K0W68_000552"
     CDS             complement(62313..62762)
                     /gene="alaE"
                     /locus_tag="K0W68_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBI3072212.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT        14865 a        16279 c        17013 g        14988 t
ORIGIN      
        1 cgatggcata ttttatgttg tcattaatga tttcaataat atgtggaagg tgatggaatt
       61 tcagcaacag atctattgcc tcttgttgct ctgaatcatt taaatcgaaa aaccagtcac
      121 caccgctggc aagccacata tttccatcgc cagtttcgaa tgcatccaac gccatcgctt
      181 cgtggttgcc tttaaccgac gtaaaccagg gttggtttag caggcgcagc acgttaagac
      241 tcttcggccc acgatcaata ttatcgcctg tggaaataag taagtcggtt tcagggtaaa
      301 aagagagttg atgttaacgg gattgtaata actgatattc accatgaata tcaccaacga
      361 cccatatatg gcgatagtga tgggcattga ttttttgata gcgtgtagat ggcatggttt
      421 taccctgtaa aataagcttt cctattatac agggtatttt tatttgattc gtcagttgtc
      481 gttaatattc ccgatagcaa aagactatcg ggaattgtta ttacaccaga ctcttcaagc
      541 gataaatcca ctccagcgcc tgacgcggag tcagtgaatc cgggtcgaga ttttccagtg
      601 cttcgaccgc aggcgaagtt tcttctggca ctgacagcaa agacatctgc gtaccatcca
      661 cttgcgtcgc ggcggcgttc ggcgaaatgc tttccagctc acgcagtttt tgccgtgcgc
      721 gcttaataac ctctttcggt acgcccgcca gagcagcaac cgccagaccg tagcttttgc
      781 tcgccgcgcc gtcctgcacg ctgtgcataa aggcgatagt gtcgccgtgc tccagcgcat
      841 cgagatgtac gttggcgacg ccttccattt tctccggtag ctgggtcagc tcgaaatagt
      901 gggtggcaaa cagcgtcaat gccttaatct tattcgccag attttctgcg cacgcccacg
      961 ccagcgacag accatcgtag gtggacgttc cacgcccgat ctcatccatt aacaccagac
     1021 tgtattcggt ggcgttatgt aaaatattgg cggtttcagt catctccacc ataaaggttg
     1081 agcgcccgga agccaggtca tctgccgcgc ctacgcgggt aaagatgcga tcgataggtc
     1141 caatctcgac tttttgtgcc ggtacatagc tgccgatgta ggccatcagc gcaatcagtg
     1201 cggtctggcg catataggta cttttaccgc ccatgttcgg accggtgatg atcaacatac
     1261 ggcgctgcgg cgacagattc agcgggttag cgataaatgg ctcattcagt acttgttcaa
     1321 ctaccggatg gcgaccttcg gtaatgcgaa tgcccggttt atcaatgaag gtcgggcagg
     1381 tgtagttcag ggtataggcc cgttccgcca ggttcaccag cacgtcgagt tccgccagcg
     1441 cgctcgcgct ctgttgcaac gcttccagat gcggcaacag caggtcgaac aactcttcat
     1501 aaagctgttt ttccagtgcc agtgctttgc cttttgaggt gagaacttta tcttcgtact
     1561 cttttagctc tggaatgatg tagcgctcgg cgtttttcag cgtctggcga cgcatgtagt
     1621 tgatgggtgc cagatggctt tgcccacggc tgatttgaat gtagtagccg tgcaccgcat
     1681 taaagccaac tttcagcgtg tccaggccgg tacgttcacg ctcgcggact tccagacgct
     1741 ccagataatc ggtcgcgccg tcagccagcg cgcgccactc atccagctct tcgttatagc
     1801 ccgatgcgat aacaccaccg tcgcgtacca gcaccggcgg tgtgtcgatg attgctcgct
     1861 ccagcagatc gcgcagctcg gcaaactcgc ccatcttctc acgtagcgcc tgtaccggtg
     1921 cactatcgac agtttctaac tgcgcacgca gctccggcag ttgctggaaa gcgtggcgca
     1981 tacgggccag atcgcgtggg cgagcagttc gtaaagccag acgtgccaga atacgttcca
     2041 ggtcgccgac ctgacgcagt accggctgca actcggcggt gaaatcctgc aatgcgccaa
     2101 tagtttgctg gcgctcaagc aacacgcggg tatcgcgcac tggcatatgc agccagcgtt
     2161 tcagcatacg actacccatc ggcgtgacgg tgcagtcgag cacagaagcc agcgtatttt
     2221 ccgcaccgcc cgccaggttc tgagtaattt ccaggttacg acgtgtcgcg gcatccataa
     2281 tgatgctgtc ctgctcacgt tccatggtga tagaacgaat atgcggcagg gtcgtgcgtt
     2341 gggtatcttt cgcatactgc aacagacaac cggcagcaca aagtccgcgc ggcgcgttct
     2401 cgacgccaaa accgaccaga tcgcgggtgc caaattgcag attcaactgc tggcgcgcgg
     2461 tgtcgatttc aaactcccac agcgggcgac ggcgcaggcc gcgacggcct tcaatcagcg
     2521 acatctcggc gaaatcttct gcatacagca gttccgccgg attagtgcgt tgcagctctg
     2581 ccgccatcgt ttcgcggtcg gccggttcgc tcaggcgaaa acgcccggag ctgatatcca
     2641 gcgtcgcgta gccgaaacct ttgctgtcct gccagatagc cgccagcagg ttgtcctgac
     2701 gctcctgtaa cagggcttca tcgctgatgg tgcctggcgt aacgatacgc acaactttgc
     2761 gctcaaccgg acctttgctg gtcgccggat cgccaatttg ttcgcagatg gcaacggact
     2821 ctccctgatt caccagtttg gcgagatagt tttccaccgc atggtaggga atccccgcca
     2881 tcgggatcgg ctctcccgcc gaagcaccgc gtttggtcag tgaaatatcc agcagttgcg
     2941 acgcgcgttt tgcgtcgtca taaaacagtt cataaaaatc acccatccgg taaaacagca
     3001 ggatctcggg atgctgggct ttcagcctga gatactgctg catcatgggc gtatgggcgt
     3061 cgaaattttc tattgcactc atggggttat gtccggttcc ctgatattaa atggggtgtg
     3121 atggttttta ttttcgttgt aatcacataa ggcgcacatg ataccggagt taatcatttc
     3181 aggggaagaa gcgaacggcg agttcttgat gctggttccg ggaaagatgc tgttttgcag
     3241 cttttgcgtg atgcttatga agcaagtacg caaaattgcg tcggttgaat aaacctcctc
     3301 cttcagagcc atagtcccct ttcaaattaa gttgtaatat aaatacacct taagagtatg
     3361 gaggcagcat gtccggcaag cgtatctctc gtgaaaaact gacgattaaa aaaatgatcg
     3421 atctttatca agcgaaatgc ccgcaggcgt cagcggagcc ggagcattac gaggcgttgt
     3481 ttgtttacgc gcaaaagcgg ctggataaat gtgtgttcgg cgaagagaaa ccagcctgta
     3541 agcagtgtcc ggtgcactgt taccagcctg caaagcgtga agagatgaaa cagattatgc
     3601 gttgggccgg gccgagaatg ctgtggcgtc atccgatctt aaccgtgcgt catctcatcg
     3661 atgacaagcg tccggtacca gaactgccgg aaaaatatcg tccgaaaaaa cctcgtgaat
     3721 agtatgccgg acaggcagat ctgtccggca atttgcagtt aaatcaatgc cgatttatca
     3781 attcccagcc gcttcatccg tgacagcagg gtcgtgcgtt tcagccccag acgttgcgca
     3841 gcgcctttag gcccggcaac cacgccgttg gtttctttca gcacgcgcac aatcaactga
     3901 tattcatctt cgccctcctg ggcgacaacc gttgcggcag gcggcgtttc aggttccggt
     3961 aaaacaatat ctggcaatga cagctgcagc acgttaccgc gtgttagcaa taccgcgcgc
     4021 tcaatgacgt tttccagttc gcgtacgtta cccggccact ccatgttgct caaggtgcgc
     4081 agcgtctcgg caggaatgct gtcgatattg cgccccagac gacgggcaat tttgaaggta
     4141 aaggctttcg ccagcagcgg aatatcttcc ggacgctcgc gtagtggcgg caggtgaatc
     4201 gggaatacgt tcaggcggta atagagatcg ctacggaact ctcggtcggc gaccattttt
     4261 ttcagatcgc ggttagtcgc ggcaattaaa cgtacgtccg tctgaatgat tttgttgctg
     4321 ccgagacgtt caaactcctg ttcctgcaat acacgcagca acttcggctg taactccagt
     4381 ggcatatcgc ccacttcgtc gaggaacaga gagcttttat ccgccagttc aaaacgacca
     4441 atgcgctggg cgctggcacc ggtaaaagcc ccacgctcat gaccaaacag atcgctttcc
     4501 agcaatccgg caggcatcgc cgcgcagttc attttgacca tgcggcgatt attacgccca
     4561 ctgagattat ggatcgcgcg ggcaatcagc tctttacccg tgccagtttc accgaggatc
     4621 agcacggtac tgtcactttg cgccaccatt tcaacttgtt taagcacgct gtacatggct
     4681 tcgctgcggc caataatctc gccaaattca ctatcaacat tgttgagctg ctcggtcagg
     4741 gcgaggtttt catcaaccag ccgttctttc agacgatgga tttcctgata ggcgagggcg
     4801 ttatcgacag cgattgccac acgttcggca atctggcgca gcaaattcag attggtagtg
     4861 gtaaacactt tctcttcgca ttgcgccagt ttcagcacgc ccagcatggt gtcgccagac
     4921 atcagcggta acaggcacaa ggtttgaatc tggttgccct aggtgtcgaa caacatgcgt
     4981 tcataggggg ctaaatcgtc ccgttcgtgg agattgatca gcagcatctc tttacttttg
     5041 aacacgcgtt cggtgagggt tccggcttca tcgacttcgc tctgttcgtg ggcgggatgt
     5101 tgtttatcaa gatagtgagt ggagtagatg ttgagtttgt ttttacggtg gctgcgtaag
     5161 acgatactga tatcgtcaat gtcgaaatag taatggattt ctttggcgac ttcgctgacc
     5221 agttcgtcca tatccaggcg ggaaagcacc gcgttggtaa tggcaaccag gatgcggaag
     5281 ttatcgcgct cccggcataa caactcatag tcgacattgt tgacgacgcg gctctggatt
     5341 tgttcggtga cgacagaaac gatctgcgta aatgtttgca gacgattgaa ctctttttcg
     5401 ctccagggac gatcgtcata acgaataaat tcacagccac caaaaatatg cccttccgcc
     5461 gccagtggca tcaggcaata gtgaccaaat ttggggtata gcccacctgc gaccagctgc
     5521 ggccaggttt cacaaaattc ttcgtaactg caatgcagcg tatcagggcg cgacaaaatg
     5581 cggcgtaccg gaccgtgtgc cagaacagtt tcgtcttcat atttaatggg gtgtcttttt
     5641 cacgcgatgc gtaataagac gcacgttgag tctgcgcttg ccacaacaca atagccgcgt
     5701 tgtcggcgag cgcagaacgc tttaccagtt gcgaaagagc ctcacacagc gaggccagat
     5761 cgggctgctg caatagtgtc cgagtgatgt cgaacaaccc ttgctgtccg agatcactca
     5821 tcggtgtata tgacattttg ttcttccagg aaagcaccgc aaagcggtgc aaaaggtcat
     5881 tgactaacta taggagattt agaattttat tagcatatac gcggaagagg ttcggcgtgt
     5941 ggtaaatcga gggttcgttt cacaccatac agaccggcaa gacgaacacc tttacgttcc
     6001 accacttcac caattagcgc cgcgtctttc cccagtggat gggaatgtaa cgctgccagc
     6061 acttgctctg ccgcgttgcg ttcaacggcg atcaccagtt tgccttcgtt ggcaaagtta
     6121 agggcgtcca gtcccagcag ttcgcaaacg ccgcgcacgg caggtttaac cggcagtgcc
     6181 acttctgaaa tttcaatacc acaaccgcag gctgccgcga actcatgaac taccgcgttc
     6241 acaccaccac gggtggcatc acgcagcgct ttcacgccgg gaatgtcacg cagcgtctga
     6301 ataagcggcg tcagtaccgc gcagtcgctg accagttcgc catccagccc cagctgctca
     6361 cgcaggttaa ggatagtcgc cccgtggtcc ccgagcgtgc cgctaaccag taatacatcg
     6421 cctgcggtta gcgtctgtgc gccccagtga atattcgccg gaattgcgcc catgccagcg
     6481 gtgttgataa acagtttatc tgccgcgccg cgctgcacca ctttagtatc gccagtaacg
     6541 atggcaatgc ctgccgcgcg cgcggtttct gccatgctgg tcactacggc tttcagtgtc
     6601 tccatcggca atccttcttc gaggataaag ccacaggaga gatagcgcgg aatagcgcca
     6661 ctgaccgcaa cgtcattggc tgtgccgcaa atcgccagct tgccgatatt accgccagga
     6721 aagaacagcg ggtcaatgac gtaactgtcg gtggagaacg ccagacggtc gccttctgct
     6781 accagctgcg ccagatcaag acgtgcctgg tcttcctgct ctgccagcca cgggttggca
     6841 aaggcttcca taaacaggct gttgattaat tgctgcatcg cctggccgcc gctgccgtgg
     6901 gcgagttgga tattattcac gcttcattct cctgctgacg atactgatac cacgcggcgc
     6961 acgctccttc ggaggagacc atcagtgcac caaatgcggt ttgaggatta caggtgttac
     7021 caaacagcgg gcattgatgc ggcttacatt tgcccgttaa tacctcacca caacgcgcgc
     7081 gcgggtcatc gcagacctgc tgcggtgctg ggcggaaatg tgcttcggca tcgaatcgtt
     7141 gataatccgg cgtcaggtgc acgccagaag attcaatcac gcctaagccg cgccattcgc
     7201 tgtcgccgtt gacacagaac acatcagcaa ttgcctgttg cgccagcagg ttaccggcat
     7261 ccggtaccac ccgacgatac tgattctcta ccttgctgtg ggccgctatt ttctgctcca
     7321 ccagcatgac cacgccttgc agtagatcaa ggggttcgaa accagcgacc accagcggac
     7381 gatgaaaatc gctggcgata aaattatagg catcagtgcc gataaccata ctaacgtggc
     7441 ccggcgcgag gaacgcgtcg ataccgttat ccggctgttc cagcaaactg cgcagcgtcg
     7501 ggataagcgt aatatgctgg cagaagaagt aaaaattctg cacatcgcgg gctttcgcct
     7561 gttgcagggt gatggcggtg gtcggcatgg tggtttcaaa acctaagccg aagaatacga
     7621 ctttacgggt tggattctcc tgcgccagtt tcaacgcatc catcggcgag taaacgatgc
     7681 gcacatcggc accgcgtgct tttgcctgca acagcgatcc ctgtttcccc ggcacgcgca
     7741 tggcgtcgcc aaaggtacag aagatgactt ccggatggct ggcaatctcc acgcaggtgt
     7801 cgattctacc catcggcagt acgcacaccg ggcaacccgg accgtggata aactcaacgt
     7861 tttcaggcag taactggtcg aggccgaatt taaaaatggc gtgggtatga ccaccgcaca
     7921 cttccataat ccgcagaggg cgttcggcgg tgtaagagag atgtgaagca cgttcgcgca
     7981 gatgctcaat taactgcatc acctgttccg gcgcgcgata ttcatcaaca aaacgcatta
     8041 tttttcctcg ccatacaaca gcgcgccgac atccggctca acgtcaaaca tgttttgtaa
     8101 ggcgtcgaga gtgtcgcgtg cttcggcttc attaattacg ctcatggcaa agccaacgtg
     8161 taccagtacc cactggccca cgcgcggctg accgttttca tcgcagctgc cgactaacgt
     8221 taaatcgaca tcgcgctgaa tgccgcagac gtcgactttt gcctggttgc cgtcaatggt
     8281 gcggatctgg ccgggaacgc ctatgcacat cgctgtgtct ccagccagtt cagccactgg
     8341 tccatccctt cgccgctggt ggcggaaata aggatgattt caatttctgg attgacttcg
     8401 cgggcgcagg cgatgcactt ctcaacgtca aagttgagat acggcaacag gtcaactttg
     8461 ttgagcagca tcagcgaggc ggcggcaaac atatgcggat atttcagcgg tttgtcttca
     8521 ccttcggtaa cggaaagcac cgccactttg tgtttttcac cgagatcgaa gctggccggg
     8581 catacgaggt tgccaacgtt ttcgataaac agaataccgt tatcgtccag tggcagacgc
     8641 ggtgcggcgt cggcaatcat ctgtgcgtca agatggcagc ctttaccggt gttcacctga
     8701 atcgctggtg tgccggtagc gcgaatgcgt gcggcatcgt tcacggtttg ctggtcacct
     8761 tcaataaccg cgcacggaac gctgtctttc aggcgcatta gggtttccgt cagcagggtg
     8821 gttttaccgg aaccagggct ggaaaccagg ttgagcacca gttgcttgcg ggcagcaaag
     8881 cgcgcgcggt tgcgttcagc cagacggtta tttttgtcca gcacgtcaat ttcgacttcc
     8941 agcatccgac gctggctcat gcccggcgcg tgagtgcccg cttcgccatg accgtaatgc
     9001 aggtcgcctt cttcagtctg gctgggggta aattcaggcg ctttaatgcc ggtgattttc
     9061 atcttcgggc gtgccgccgg ggcaaatggc gcgctacgaa acgcggaatg agggttatgt
     9121 tcatcaccct cgatatacag gttgccttca ccgcaaccgc atgttgtaca catcgctcac
     9181 tcctggtcta tttctatccg ccgaatctgt aaaccgtcgt ctgccacaat ctgtagcatg
     9241 tcaccatgac actgtggaca gcggcggacg cgctgggtca gtagcgtcac atactgttgg
     9301 catgtttcac accagcattc ggcctcttgt tcttcgaggt gcagtttaca accttccgcc
     9361 acgctgccgc ggcaaaccag atcaaaacaa aaggcaagag agctggtttc gacacaagaa
     9421 aatgcgccaa ttttgagcca gaccccagtt acgcgttttg cgccgtgttt tgcggcctgc
     9481 tgttcgatca attccagtgc ccgttggcag agggttattt cgtgcatatc gcctcccatt
     9541 aactattgcc agctacaagc aataattgtg ccagtgttga ttatccctgc ggtgaataat
     9601 gtcgatgatg tcgaaatgac acgtcgacac ggcgacgaaa ttcatcttta gcttaaaaat
     9661 ctatttaata acaataaatt aaaagttggc atagaaaatg cttaaagctg gcatctctgt
     9721 taaacgggta acctgacaat gactatttgg gaaataagcg agaaagccga ttacatcgca
     9781 cagcggcatt gtcgcctaca ggaccagtgg cacatctact gcaattcgct ggttcagggg
     9841 atcacgttat cgaaagcgcg cctgcatcac gccatgagct gcgcgccgga caaagaactc
     9901 tgtttcgtcc tttttgaaca ttttcgtatt tacgtcaccc tggcggatgg ctttaacagc
     9961 cacaccatcg agtattacgt cgaaacaaaa gatggcgaag acaaacagcg gattgcgcag
    10021 gcgcaactga gcattgacgg catgattgat ggcaaggtca acatccgcga tcgcgaacag
    10081 gttctggaac actatctcga aaaaatcgct ggcgtttacg acagcttata caccgctatt
    10141 gaaaacaatg tgccggtgaa tttaagccaa ctggtaaagg gacaaagccc ggcagcatga
    10201 gctgaggctt tgcccgtttt gcaggcgtta cgcctgtttg gggatgggcg tgtcgatgag
    10261 tgtcgaaaat gacatttcat cggcatgttt tcgtcaaaaa tgacaatcac ctgaggaatg
    10321 cctggtgaat cgttttgtaa ttgttgactc cacgctctgt atcggctgcc acacttgtga
    10381 ggccgcctgt tcagagacgc atcgccagca cggcctgcaa tcaatgccgc gcctgagagt
    10441 gatgctgaat gaaaaagaat ctgcgccgca gctctgtcac cactgtgaag atgcaccctg
    10501 tgcggtggtt tgcccggtta acgccatcac ccgcattgat ggggccgtgc agttgaatga
    10561 aagtctgtgc gtaagctgca aactgtgcgg catcgcctgc ccgtttggcg caattgaatt
    10621 ttccggcagc cgtccgctgg atattccggc aaacgccaat accccgaaag cgccaccggc
    10681 accgcctgct ccggcgcgta tcagtacgct gcttgactgg gtgccgggta ttcgcgcgat
    10741 cgccgtcaaa tgtgaccttt gcagttttga tgaacaaggt ccggcctgcg tgcggatgtg
    10801 cccgactaaa gccctgcatc tggtggataa caccgatatc gcccgcgtca gcaaacgtaa
    10861 gcgtgagctg acctttaaca cggactttgg cgatctcacc ttgtttcagc aggctcaaag
    10921 tggagaggct aaatgagcgc aatttccctg atcaatagcg gcgtggcatg gtttgtcgcc
    10981 gccgctgttc tggcatttct cttttctttt caaaaagcgt taagtggctg gatagctgga
    11041 attggcggcg cggttggtag tctgtatacg gcagccgcgg gcttcactgt actgactggc
    11101 gcggttggcg tgagcggtgc gctgtcgctg gtaagctacg atgtgcaaat ctctccgctt
    11161 aacgcgattt ggctgattac gctcggtctg tgcggtctgt ttgtcagcct ctacaacatt
    11221 gactggcatc gccacgcgca ggtgaagtgc aacggcttgc agatcaatat gttgatggct
    11281 gccgccgtct gcgccgtcat cgccagcaac ctcggcatgt tcgtggtaat ggccgaagtc
    11341 atggccttgt gtgcggtgtt cctcaccagc aacagcaaag agggcaaact gtggtttgcg
    11401 ctggggcgtc ttggcactct gctgctggtg attgcttgct ggctgctgtg gcagcgttat
    11461 ggcacgctgg atctgtgcct actggatatg cgtatgcaac agctgccgct cggttccgat
    11521 atctggctgc tcggtgtgat tggctttggc ctgctggccg ggattattcc gctgcacggc
    11581 tgggtgccgc aggcacatgc taacgcctct gcgccagctg ccgcgttgtt ttctacggta
    11641 gtcatgaaaa ttggcctgct gggcatgtta accctgtcac tgctgggcgg taatgcaccg
    11701 ctgtggtggg ggatcgcgct gctggtgctc ggcatgatca ccgcgttcgt cggtggtctg
    11761 tatgcgttga tggaacacaa catccagcgc ctgctggctt accacaccct ggaaaatatc
    11821 ggcattatcc tgctggggct gggcgctggc gtaacgggta tcgcgctcga acaaccggcg
    11881 ctgattgctc ttggcctggt cggtggtctg taccatctgc ttaaccatag cctgttcaaa
    11941 agcgtactgt tcctcggggc ggggagcgtc tggttccgta ccggtcatcg cgatatcgaa
    12001 aaactcggtg gtattggcaa gaaaatgccg gttatctcca tcgccatgtt agtcgggttg
    12061 atggcaatgg ctgcgctgcc gccactgaac gggtttgccg gggaatgggt tatctatcaa
    12121 tccttcttca aactgagcaa tagcggcgcg tttgttgccc gtctgctggg gccgctgctc
    12181 gctgtgggtc tggcaattac cggtgcgctg gcggtgatgt gtatggcgaa agtctatggc
    12241 gtcacgttcc tcggcgcgcc gcgtaccaaa gaagccgaaa acgccacctg tgcgccgctc
    12301 ctgatgagcg taagcgtagt ggcactggcg atttgctgcg taattggcgg tgttgctgcg
    12361 ccgtggctgc tgccgatgct ctctgctgct gtacctctgc ctctggagcc tgctaacacc
    12421 accgtttctc aaccaatgat cacattgctg ctgattgcct gcccgctgct gccattcatc
    12481 attatggcga tttgcaaagg cgaccgcctg ccgtcacgtt cccgcggtgc ggcctgggtg
    12541 tgcgactacg accatgaaaa atcaatggtg attaccgctc acggttttgc catgccggtt
    12601 aaacaggcgt ttgcgccagt gctgaaactg cgcaaatggc tgaatccggt gtcgctggtg
    12661 ccgggctggc agtgcgaagg tagtgcgttg ctgttccgcc ggatggcgct ggttgaactg
    12721 gcggtactgg tggtgattat tgtttcacga ggagcctgag aatgagtgtt ttatatccgt
    12781 taattcaggc gctggtgtta tttgccgttg cgccgctgct ctccggtatt acccgcgtgg
    12841 cgcgcgcccg tctgcataac cgtcgtgggc cgggtgtgtt gcaggagtat cgtgacattt
    12901 tcaaactgct gggccgccag agcgttggcc cggatgcctc cggctgggtg ttccgcctga
    12961 cgccgtatgt gatggtgggc gtcatgctga ctatcgctac tgcgctgccg gtggtgaccg
    13021 tcggttctcc gctgccgcaa ctgggtgatt taatcacctt actgtatctc tttgccatcg
    13081 cgcgtttctt ctttgccatt tctggtctgg ataccggtag cccgtttacc gctatcggcg
    13141 cgagccgtga agcgatgctt ggcgtgctgg tcgaaccgat gctgctgctt ggtctgtggg
    13201 ttgccgcaca ggttgccggt tccaccaaca tcagcaacat caccgacacc gtttatcact
    13261 ggccgctgag ccagagcatc ccgctggtac tggcgctttg tgcctgtgcg ttcgccacct
    13321 ttatcgaaat gggcaaactg ccgttcgacc tggcggaagc cgagcaggag ctgcaggaag
    13381 gcccgctctc tgaatacagc ggcagcggct ttggcgtcat gaaatggggt atcagcctga
    13441 aacagctggt ggtgctgcag atgttcgtcg gcgtatttat cccgtgggga cagatggaaa
    13501 ccttcaccgc cggtgggctg ctgctggcgc tggtgattgc catcgtcaaa ctggtcgtcg
    13561 gcgtcctggt tatcgcgctg ttcgaaaaca gcatggcccg tctgcgtctt gatattactc
    13621 cgcgcattac ctggactggg tttggctttg catttttagc gttcgtctcc ttgctggcgg
    13681 cgtgattaaa gagagtttga gcatgtctga agaaaaatta ggtcaacatt atctcgccgc
    13741 gctgaatgag gcatttccgg gcgtcgtgct ggaccacgcc tggcagacca aagatcagct
    13801 gactgtcacc gtaaaggtga actatctgcc ggaagtggtg gagtttctct actacaagca
    13861 ggggggctgg ctgtcggtgc tttttggtaa cgacgaacgc aaactgaatg gtcattacgc
    13921 cgtttattac gtgctgtcga tggagaaggg gactaagtgc tgggtaacgg ttcgcgtcga
    13981 agttgatgcc aacaaaccgg agtatccgtc cgtgacgccg cgcgttccgg cggcggtgtg
    14041 gggcgagcgc gaagtgcgcg atatgtacgg tttgattccg gttggtctgc cggatgaacg
    14101 ccgtctggtg ctgccggatg aactttatcc gctgcgtaaa gacagcatgg attatcgtca
    14161 gcgtccggca ccgaccaccg atgctgaaac ctacgagttc atcaacgaac tgggcgacaa
    14221 gaaaaacaac gtcgtgccga ttggtccgct gcacgtcact tctgacgaac cgggccactt
    14281 ccgtctgttc gtcgatggcg aaaacattat cgacgccgac taccgtctgt tctacgttca
    14341 tcgcggcatg gaaaaactgg cggaaacccg tatgggttac aacgaagtga ccttcctctc
    14401 tgaccgtgtg tgcgggatct gcggctttgc ccacagcacc gcctacacca cgtcggtaga
    14461 aaacgcgatg ggtattcagg tgccagaacg tgcgcagatg atccgcgcca ttctgctgga
    14521 ggtggaacgc ctgcactcgc atctgctcaa cctcggtctc gcctgccact ttaccggctt
    14581 tgactccggc tttatgcagt tcttccgcgt gcgtgaaacc tccatgaaaa tggcagagat
    14641 cctcaccggt gcgcgtaaaa cctacggcct gaacctgatc ggcgggattc gtcgcgatct
    14701 gttgaaagat gacatgatcc agacccgcca gctggcgcaa cagatgcgtc gtgaagtgca
    14761 ggagctggtg gatgtgctgc tgagtacgcc gaacatggaa cagcgcaccg tcggcattgg
    14821 tcgtctggac ccggaaatgg cccgcgattt cagtaacgtt ggcccgatgg ttcgcgccag
    14881 cggtcacgcc cgcgatactc gcgccgatca cccgtttgtc ggttatggcc tgctgccaat
    14941 ggaagtccac agcgagcagg gctgcgacgt tatttcccgt ctgaaagtgc gtatcaacga
    15001 agtttatacc gcgctgaaca tgatcgacta cggtctggat aacctgccgg gcggtccgct
    15061 gatggtggaa ggctttacct acattccgca ccgtttcgcg ctgggctttg ccgaagcgcc
    15121 gcgcggcgac gatatccact ggagcatgac cggcgacaac cagaagctgt accgctggcg
    15181 ctgccgtgcc gcgacctacg cgaactggcc gaccctgcgc tacatgctgc gcggtaacac
    15241 cgtttccgat gcgccgctga ttatcggtag cttggaccct tgctactcct gtaccgaccg
    15301 catgaccgtg gtcgatgtgc gtaagaaaaa gagcaaagtg gtgccgtaca aagaactcga
    15361 gcgttacagc attgagcgta aaaactcgcc gctgaaataa ggaatcgcca tgtttacctt
    15421 tatcaaaaaa gtcatcaaaa ccggcacggc gacctcgtct tatccgctgg agccgattgc
    15481 ggttgataaa aacttccgtg gtaagccgga gcagaacccg cagcagtgca tcggctgtgc
    15541 ggcctgcgtc aatgcctgcc cgtcaaacgc cttaacggtt gaaactgacc tcgccaccgg
    15601 tgagctggcg tgggagttta atctcgggcg ctgcatcttc tgtggacgct gcgaagaagt
    15661 ttgcccgacg gcggcgatca aactgtcgca agagtacgaa ctggcagtgt ggaagaaaga
    15721 agacttcctg caacagtccc gcttcgcgct gtgcaactgc cgcgtctgca atcgtccttt
    15781 cgccgtccag aaagagatcg actacgccat tgcgctgctt aagcacaacg gcgacagccg
    15841 cgcggaaaac caccgcgaaa gctttgagac ttgcccggaa tgtaagcgcc agaaatgcct
    15901 ggtgccgtcc gaccgtattg aactgactcg ccatatgaaa gaggccatct gatgagcaat
    15961 ttattaggcc cccgtgacgc caacggcatt ccggtcccga tgacggtgga tgaatccatc
    16021 gccagcatga aggcgtcgtt actgaaaaaa atcaaacgtt ctgcctacgt ttatcgcgtg
    16081 gactgcggcg gctgcaacgg ctgcgaaatc gaaattttcg ccacgctttc gccgctgttt
    16141 gatgcagaac gcttcggcat taaagtcgtt ccttcaccgc gtcatgcgga tattttactg
    16201 tttaccggcg cggtcacccg tgcaatgcga tcccctgcgc tgcgtgcgtg gcagtccgcg
    16261 ccggacccga aaatttgtat ctcctacggt gcctgcggta acagcggtgg tattttccac
    16321 gatctctact gcgtgtgggg cggtacggat aaaatcgtcc cggtagatgt ctacattcct
    16381 ggttgcccgc caacgcctgc cgccacgctg tacggctttg caatggcgct cggcctgctg
    16441 gagcagaaaa ttcacgcccg tggcccgggt gagcaggatg aacaacaggc ggaaatcctg
    16501 catggcgata tggtgcagcc gctgcgtgtg aaagtggatc gcgaagcacg ccgcctggcg
    16561 ggttatcgtt acggtcgtca gattgccgat gacttcctga cacagttagg gcagggcgaa
    16621 gaacaggttg cacgctggct ggaagcggaa aacgatccgc gtctgaacga gattgtcagc
    16681 catctgaatc atgttgttga agaggcgcgt atccgatgag tgaaaaggtg gtgttcagtc
    16741 aactgagccg taaatttatt gatgagaacg atgccacgcc cgccgaggcg cagcaggtgg
    16801 tctattacag cctggcgatt ggtcaccacc ttggggttat cgattgcctg gaagcggcgc
    16861 tcacctgccc gtgggatgaa tatctggcat ggattgccac tctggaggcg ggcagtgaag
    16921 cccgccgcaa aatggaaggc gtgccgaaat atggtgagat cgtcatcgac attaaccatg
    16981 tgccgatgct ggccaacgca ttcgataaag cccgggcagc gcaaacttcg cagcagaagg
    17041 aatggagtac aatgctgtta agtatgctgc atgatattca tcaggaaaac gccatctatt
    17101 tgatggtgag gagactgcgt gactgacgtt ttactctgtg ttggcaatag catgatgggc
    17161 gatgatggcg ctggcccgct gctggcggaa aagtgcgccg ccgcgccgaa aggtaactgg
    17221 gtggtgattg atggcggtag tgcaccggaa aacgacatcg tcgccatccg tgaactgcgc
    17281 cctacgcggt tactgattgt tgacgctact gatatggggc taaaccccgg cgaaatccgc
    17341 atcatcgacc cggatgatat cgccgagatg tttatgatga ctacccataa catgccgctg
    17401 aactatctta tcgaccagtt gaaagaagac attggcgaag tgattttcct cggcatccag
    17461 ccggatatcg tcggctttta ctacccgatg actcagccga ttaaagatgc ggtagaaacc
    17521 gtttatcaac gactggaagg ctgggaaggg aatggcggct tcgcgcagtt agcggtggaa
    17581 gaagaataat tctccatcag ggaattcagc gaaatgaata tcagaacagg gatgtgcgct
    17641 tttttattga gcttagtgct gcctgcacag gccaccagct ttaccgaata tctgccaatg
    17701 agtgacagcg aatacgcgca aaagcgggcg ctgaaacctc tcctgaccat gccttatgat
    17761 gccgagcaaa cctggcattt tagaaaagtg ggcgttgctg gcgtgacgct tgaaaagatg
    17821 ccaaatgatg atagtgaatg gcaattgaac ggtaaagatc gcgccggtaa atcctggtct
    17881 gtgcctgtgg gtatgctggc aaacatagcg ggcaaagggc aactctatcg cgccgatctt
    17941 gatcgtaacg gcattcagga tctggttatc tggttaccaa gtaccggtaa tggtctggct
    18001 ccttatgcac atttaattct tatgacgttc acgcgcgaag gccgtccctg tgtctttgaa
    18061 cccaggggat tctacaccgc aagcaaaact ggcgtagatg atttgctgga tttgcaaggc
    18121 aatggtcaca cgcaattgct ggatatgcag tttgattccg gttactggat aaccagtctg
    18181 tatcaggtaa aagacgccaa atggcagcgt gttcacggct ggtttggcaa gttaagctat
    18241 cccgcgctca cgcgttttac ttataccccc aaccgtaaac tcgtcctcaa acccatcgcc
    18301 gggcgcgacc cgcaaacgga agatctggcg cagacgcagc gttgcttgat aaagggggat
    18361 gttcttgatg gggtgaacca gaactgactt tttgcagagt ggctatgagg attttggggg
    18421 taattatatc gcgctcaagt gtttacatat tgtgaacttg gatttgatga gctatagttt
    18481 ccgaacacat aaggatagaa ctcgcagatg tggctgttta cttactataa ggaagttaaa
    18541 acttaaaaat gcacatcatt tctaaagcgc catttgaaga gtctgcgcgt aaatatccta
    18601 acgacgctct ggctcttcag gcattgtatc gggttattaa ggaaacggat ttttctacgc
    18661 cagaagagat gcgaacggcg tttcccaacc tcgacaattt taaatatcgc aacaaatggt
    18721 acgttctgga tgtaggtggg aacaacctaa gggtgattgc ctacataaac tttgttaata
    18781 agcggttctt tgtgaagcac ataactaatc acgccgagta tgacaaactg acacgttact
    18841 acagagagaa caaagaatga ctgctaatgc tgcacgcgct gttaaagcaa caagagaact
    18901 ggtcaatgca gtcccctttc tgggaggtag tgactcagaa gatgattatc gtgaagcact
    18961 ggaactggtc gaatatttga ttgaagaaga tgacaccaat ccacttatcg actttctggc
    19021 gagccgcatt gcagaatatg aaaacaacaa ccaagagttt gcagagttcg acaaagccgt
    19081 tgcggcaatg cctgttggcg tggcgttact tcgcacgctg attgatcaac ataatctgac
    19141 ctatgcggat ttgaagaacg aaatcggctc taaatccctg gtcagtcaga ttttgtctgg
    19201 acaacgatcc ctgacgatat cccacattaa ggcactttca gcccgatttg gcgttaagcc
    19261 ggaatggttt ctttgatttt gactgatgcc aaaccccaga ttgcccgatg cgctgcgctt
    19321 atcgggcctt cctggttcgt gcgacatgta ggccggataa ggcgttcacg ccgcatccgg
    19381 cactgttacc tactctaaat cttccccatt actggcaatc acttttttat accaccagaa
    19441 cgatttctta cgtgtgcgcg tcagcgtgcc gttgcctgcg tcgtcacggt ctacgtagac
    19501 aaaaccgtag cgtttgctca tttcacccgt agaggcggag actaaatcaa tacagcccca
    19561 ggtggtgtag cccatcagcg gaatgccatc tgcaatcgct tcgcccattg cgcggatatg
    19621 ttcgcgtaaa tagctgatgc gatagtcatc gttaatctcg ccattggcag caagttcatc
    19681 ttttgcgccg aggccgtttt ccaccagaaa cagcggcttc tgataacggt cgtacatcat
    19741 gttcatggtg atacgcagac cgagtggatc aataccccag ccccagtcgc tcacttgcag
    19801 atacgggtta cgcagcgatt tcactacgtt cgccgcactg ctattgttgg cgttcatctc
    19861 cgccgaggcg cagcgcgagg cgtaatagct gaaagagaca aaatcgacgg tgtttttcag
    19921 gatttcatca tcgcccggtg ctttgtcgat ggttacccct ttttcgcgga atacgcgggc
    19981 agagtaagcc ggatacgtgc cccgcgcctg cacatcgata aaaaacaggt tttcccggtc
    20041 tttctccagc gccgcccaga catcttccgg cttgcaactg taagggtaga agttaccgcc
    20101 cgccagcata cagcccacct gattttgcgg gttaacctca tgggcgattt tggtggctag
    20161 cgcactggca accagctggt gatgcgcggc ctgatatttc acctgatcct gattttcacc
    20221 ttcttcaaac accagacccg cgccggagaa cgggctatgc aacataatgt tgatttcatt
    20281 gaaggtaagc cagtatttca ccagaccatc aaatgcttca aagcaggtcc gggcgtagcg
    20341 gctgaaaaac tccaccagct tgcggttacg ccaggagcca tattcggtga ccagatgcat
    20401 cggcacatcg aagtggcaca acgtgaccag cggttcgata ccgtactttt tacactcttc
    20461 aaagacagaa cgataaaaag caatgccctg ctgattgggc gtgatttcat cgccctgcgg
    20521 aaagagacgg ctccaggcaa ttgaggtacg gaaaaccttg aatcccatct ctgccatcag
    20581 ggcgatatct tctttataac gatgataaaa atccgtcgcc tcatggctgg gataaaactc
    20641 gtcatcgcgc aactgaaaac gtttttccag ccccagtttc accgccattc gatgctcgcc
    20701 gtgtgggatc atatcgacag tggtcagccc tttgccacct tcacggaacg caccttcaga
    20761 ctggttggcg gcaagcgcgc cgccccataa aaaaccttct ggaaatactg acattttcat
    20821 cctcaattaa gacttacttc tttggcgacg gtcggttgta cgctctgatg ctttcgcgcc
    20881 tcggcagccg cttcctcaac aggaatatcc tcaaagccga gcaacagtgt gaggataaac
    20941 gacagcacca ccgccagcgc catgaccgcg aacacccaga cgatgctcat tggattcgcc
    21001 ggatcgaaga attgcacgct ggtaaataac cctggcgctg ccattgagtg gctggcaagc
    21061 cccgccatac cggcaaccgc gccgcaaata aaaccgctga taagactggc gataagcgga
    21121 cgtttcaggc ggatcgccac gccgtataac gccggttcgg aaatccccgc cataatggct
    21181 gatgccgccg cagccagcgc cgtctggcgc agttccgggt ttttcgtttt ccacgccacc
    21241 gccagtgatg aaccgcccag cgacaggtta gcgccgatct ctgacggcat gaccatccct
    21301 tctttgccgg tttcggcaat ggtctgaatg atggttggcg taaagacgcg gtgcatcccg
    21361 gtcattacca gcagaggcca cagcgcgccc ataatggcga ctgaaagcca gcccagataa
    21421 ccatgaatgg tgtaaaccag cgccgaaatg gcgctaccga tccagatacc aatcgggcca
    21481 atcagcagga ttgccagcgg tgcggcaatc aacacaatca gcattggctt gaggaagttt
    21541 ttggtcaccg ccggtgtaat gcgatccacc cagcgttcga tatatgacag gcaccaggtc
    21601 atcaccagcg ccgggatcac tgtgtaggtg tatttcaccg cggtgaccgg aatcagggca
    21661 aattcgacat gttcaccctg ggccgctttc gccatcagtt caataaaact cggatgtacc
    21721 agcacacccg cgatggcaat cgccagcgac atattggttt taaatttgat ggcggcagat
    21781 gccgcgacca tcagcggcag gaagaagaaa gcaccgtcac caatcacatt cagaatggtt
    21841 aaggtcggcg atccttttgt cagcacgccg ctcatctcga ggatcatcgc cagcagtttg
    21901 accatcgatc cgccgataat cgccgggatc agcggtgaca tggtgccgat cagcgcatcg
    21961 aggatccccg caccaatgcg acgcagcgtg agtttgggtt tacccacggg ctgtgcgggc
    22021 tgcatatctc ccggcagcag gctgacgact tcctgaaagg cttgtgaaac ggtattgccg
    22081 ataatcacct gacactggtt gtcactacgt accacgccga gcacgccggg gatggttttt
    22141 aacgtcgggc tgtcgataag ctggtcatct ttgataacaa cgcgcaaacg cgtcatacag
    22201 tgcgtgaccg ccgagatgtt atcaacgccg cccagtgccg ctatcaccga gcgtgccagc
    22261 gccgcataat ttttggccat cggattttat cctgttatca gtagggtaga tgtagttgtt
    22321 atcagtcgac gtaacggaaa aatgccgatg ctgaaacgga attaggaaac cggtttcaca
    22381 aaatcatgaa tagttgtcac taaataagca agagagttaa ttgttcattt tttatatttg
    22441 tgaaaccggt cacctgaata tttttcccgc cgattgcgca aggacaatcg gcaaacggtg
    22501 cagtacactg cgttgattgc gtgtaaaggg atgaaaatga tgacgacgat gctggaagtg
    22561 gcgaagcgcg ccggggtttc aaaagcgacc gtttcccgcg tgctttcagg taatggctac
    22621 gtcagccagg agactaaaga tcgcgtgttt caggcggtag aagagagcgg ttaccgccca
    22681 aacttgctgg cgcgcaatct gtcggcgaag agtactcaga cgctggggtt ggtagtgacc
    22741 aacacgcttt accacggcat ttattttagt gaattactct ttcatgccgc gcgaatggcg
    22801 gaagagaaag ggcggcagtt gctattggca gatggtaaac acagcgccga agaagagcgc
    22861 caggcgattc agtatctgct ggatttgcgc tgcgacgcca ttatgattta cccgcgtttt
    22921 ttaagcgtgg atgagatcga tgacatcatt gacgcgcaca gtcagccgat aatggtgctt
    22981 aatcgccgcc tgcgcaaaaa cagcagccat agcgtctggt gcgatcataa acagaccagc
    23041 tttaatgccg tggcagagtt gataaacgcc ggacatcagg agattgcttt ccttaccggc
    23101 tcgatggatt cccccaccag cattgaacgt cttgctgggt ataaagacgc gctggcgcag
    23161 catggtattg cgctcaatga aaaacttatc gctaacggta aatggacgcc cgccagcggg
    23221 gccgaagggg tagaaacgtt gctcgaacgt ggggctaaat ttagcgcgtt agttgccagt
    23281 aacgacgata tggcgatagg tgcgatgaaa gcgttgcacg agcgcggcgt agcggtgcca
    23341 gagcaggtgt cagttatcgg attcgatgat atcgcaattg ccccctatat cgttccggcg
    23401 ctctccagcg taaaaattcc ggtgactgag atgattcagg aaattattgg acggctgatt
    23461 tttatgctcg atggcgggga tttctcaccg ccgaaaacct tcagcggaaa actgatccgc
    23521 cgcgactcac tcattgctct ttcgcgataa acattgtcat cacatgtcat cgtcactagt
    23581 gtcgatgaca tcttttttcc tgcattacag aaattaattt ccttaaataa cagtgaatta
    23641 aaactggcat gatttgtgaa tgtatcggcg cattaactgt cattgctgga gaatttgatg
    23701 aaccgtttca tcattgctga cgcgagtaaa tgtattggtt gctgtacctg tgaagtggcg
    23761 tgcgtggttt ctcatcagga aaatcaggac tgtgcatcgc tgaccccgga aacattttta
    23821 ccgcgtatcc atgtcattaa aggtgtgaac atttccacgg cgacagtttg ccgtcagtgt
    23881 gaagatgcac cgtgcgctaa cgtctgcccg aatggtgcta tcagccgtga taaagggttt
    23941 gttcatgtga tgcaggaacg ttgcattggt tgcaaaacct gcgttgtggc ttgcccgtat
    24001 ggcgcgatgg aagtggtggt acgtccggtg attcgcaaca gcggcgcggg gctgaatgta
    24061 cgggctgaca aagccgaagc caataaatgt gacctgtgca accatcgtga agacggcccg
    24121 gcgtgtatgg cggcttgccc gactcatgcg ctgatttgtg tcgatcgtaa taaacttgaa
    24181 caactgagtg cagaaaaacg ccgccgtacg gcgctgatgt tctaaaaatc ttacagaact
    24241 gttctggcat caaaggaatt cccggaaata ttcgtagaat acggagctgg cggtatcgcc
    24301 gacctgagac tgccggatac gacgcagtcg tgtccggcag gacgggttac gcaattcatg
    24361 atggcgggaa taactcaatg gcaaaaaaca catcttgcgg tgtccaactg cgtattcgtg
    24421 gcaaagtgca gggcgtcggt tttcgtccgt ttgtctggca gctggcacag caattaaatc
    24481 ttcacggcga tgtctgtaat gacggcgatg gcgtagaagt ccggctgcgg gaagacccgg
    24541 aaacgttact tgttcaattg catcagcact gcccgccgct ggcgcgtatt gatagcgtcg
    24601 agcgtgagcc gtacatctgg tcacaactgc ccactgagtt caccatccgc cagagcgcgg
    24661 gaggtgccat gaatacgcaa attgtcccgg atgccgctac ttgccctgct tgccttgccg
    24721 aaatgaatac cccaggcgaa cggcgttatc gttatccgtt tatcaactgt acccactgcg
    24781 gcccgcgttt caccattatt cgcgccatgc cttacgaccg cccgtttacc gtgatggcgg
    24841 cgtttccgtt gtgtccggct tgtgacaaag agtaccgtga cccgctcgat cgtcgcttcc
    24901 acgcccagcc ggtggcctgc ccggagtgtg gcccacatct tgaatgggta agtcatggtg
    24961 aacatgcaga acaagaggcg gcattacagg cggctatcgc acagttaaaa atgggcaaca
    25021 ttgtcgccat caaagggatt ggcggatttc atcttgcctg cgatgcacgt aacagtaacg
    25081 cggtggcgac acttcgggcg cgcaaacatc gcccggcgaa accgctggcg gtcatgttgc
    25141 cagtggctga cggtttacca gacgctgcgc gccagttgct taccacgccc gccgcgccga
    25201 ttgtgctggt ggataaaaaa tacgttcctg agctttgtga tgatatcgcc cctgacctta
    25261 acgaagtcgg ggtaatgttg cctgcgaacc cgctccagca tttgctgtta caggaactgc
    25321 aatgcccgct ggtgatgacc tccggcaacc tgagcggtaa accaccagct atcagcaacg
    25381 aacaggcgct ggcggatttg cagggcattg ccgacggatt cttgatacat aaccgcgaca
    25441 tcgtgcagcg gatggatgat tcggtggtgc gcgaaagcgg cgaaatgctg cgccgttcgc
    25501 gggggtatgt gccggatgcg ctggctttgc ctccgggctt taaaaatgtt ccgcctgtgc
    25561 tgtgtctcgg cgcggatctg aaaaatacct tctgcctggt gcgcggtgaa caagcggtgt
    25621 tgagtcagca tctgggcgat ttaagtgacg atggcatcca gatgcagtgg cgcgaagcgt
    25681 tacgcctgat gcaaaacatc tacgatttta ccccgcaata cgttgtgcat gacgcgcatc
    25741 cgggctatgt ctccagccag tgggcgcgtg aaatgaatct gccgacgcaa acggtactgc
    25801 atcatcatgc ccatgcagcg gcgtgtctgg cagagcatca gtggccgttg gatggcggtg
    25861 atgtcattgc tttgacgctc gacggtatcg gtatggggga gaacggcgct ttgtggggcg
    25921 gcgagtgcct gcgggtgaac tatcgcgaat gtgagcacct gggcggcttg cctgcagtgg
    25981 cgcttccggg tggcgatttg gcagcgaagc agccgtggcg aaacctgctg gcgcagtgcc
    26041 tgcgctttgt gccggagtgg cagaattacc ctgaaacagc aagtgtgcaa cagcaaaact
    26101 ggagcgtgct ggcgcgggcc attgagcgtg gaattaacgc gccgctggcg tcatcgtgtg
    26161 ggcggttgtt cgatgcagtg gcggcggcac tgggctgtgc gccagccacg ttaagttatg
    26221 aaggtgaagc ggcttgtgct ctggaggcgc tcgcagcctc atgccacgga gtgacgcatc
    26281 cggtgacgat gccgctggtg gacaatcaac tggatctcgc cgctttctgg cagcagtggc
    26341 tgaactggca ggcaccggtt aatcaacgcg cgtgggcgtt tcatgatgcg ctggcgcagg
    26401 gttttgccgc gttgatgcgt gagcaggcca cgatgcgtgg tatcactacg ctggtattta
    26461 gcggcggggt tattcataac cgtttgctgc gtgcacgtct ggcgcattat ctcgctgatt
    26521 tcacattgct ctttccacag agtttaccgg cgggtgatgg cggtttgtct ctggggcagg
    26581 gggttattgc tgcggcgcgt tggttagcgg gtgaagtcca gaacggataa aacgtgttgc
    26641 atttggacac cattgccgga tgcggcgtaa acgccttatc tggcctacac atgcgtcccc
    26701 gtaggtcgga taagacgcgc cagcgtcgca tccgacattt taggcacagt agcccaccta
    26761 catcggcaat gttttcaaca atccaaatgc ctctttcatc cgatcctcac tgaccacaaa
    26821 ggcgcgaagc tggtcagcat cgtcaacacc gcgagccacc attccctggc gttcggtatt
    26881 aatttgccag cgtaaatcct gacgctgggt ttcgcctgcc agatgcagcg gtaattccgg
    26941 cgttttgatt ttcaccagca tcgccggtag tttcagcggc gtgttattgc cgagaagatt
    27001 ttttgccagc accatcgcgc taagttgaat cggctgcagg aatggcagta cctgaccgtt
    27061 gatttccgcg caatcgccca gcgcataaat atcgtcatta ctggtttgca gataactatc
    27121 gacgcaaacg ccgcgattaa tcgtcagccc ggcgcgtcgt gccagggctg tttccgggcg
    27181 cagtccggtg gcggcaatta ccgcatccac ttcgatacta cgctggtggt ccagcgttgc
    27241 cagaatgcca gaatccgttt tttccagccc ctgcaactga gatttcaaca gcagatgaac
    27301 gcccatctcc gtcaaccgat gctgcaagcg gctgcttact tctggtggca ttaatgacgc
    27361 cagaatactg gcagcgttgt cgattagcgt gaccgctttg cctgcacgac aaaaatccat
    27421 cgccagttcg ctaccaatca aaccaccgcc gacaatcaac acgcgtcggg catcccgcag
    27481 ttgcgtttca caggcgcgat actcttgctg actatttaac gtcagcatta actcacgccc
    27541 aggcactggc gggacaaagg cactggcacc agttgccagc accagcttgt cgtattgcca
    27601 ctgattattc tggcttttca ccacatgggc ttcggcatcg atatccgtca cccaggtgtg
    27661 cggaaacagg tgcagattaa actgcccggc aaattcaccc gccgtctggc gggtaaggtc
    27721 gtcggcacgt tgcccctggc tgataacatg gctgaggtcc ggtttgttgt actcatccat
    27781 gctgtcggcg gcgatcaggg ttaatggaat actggcgtcc tgtttgcgaa tatttttcac
    27841 cagttggcgg gcggcgaagc ccgaaccaat gatcacaatg ccgttgctca ttttgcctcc
    27901 gatgccagtt cgtcaaagac gtctttgccg agagagcatt ccgggcagag gaagttatcc
    27961 ggaacttcac tccacggcgt tcctggcgca acgtcctgca ttggctcgcc ttttgccgga
    28021 tcgtaaatcc actggcagac gctgcactgc atccgtgggc cgaggtcgga cttcgtggtg
    28081 gcagaggttt cttctttaac caccgtattc accgtgctct gcggcagcgg cgcgagcgcc
    28141 cactgacggg cgatttcgcg accatgttca cggcataact ccagagcgtc ctggtctggt
    28201 cgccatttgg ctttcaggct aagcgacatt tcgaaacccg catcctgcag gcgcgtggaa
    28261 agacgatcca ccgcaccgcc gctccagccg tgagagccga aagcactggc gcgtttgtta
    28321 cggaagcgta atccggtcat ctcctccacc agaccggcga ttttcggcat catcacgtta
    28381 ttcatcgtcg aagtgccgac cagcacgcct tttgagcgga agacattggt caggatttcg
    28441 tttttatcgc ttcgggcgac gttgaaaatt ttcaccgcca cgcgtgggtc ggtttccgca
    28501 atcccctggg cgatagcgtc agccatcatg cgggtgttat tcgacatggt gtcgtagaaa
    28561 atggtgattc tgtcttcctg ataatcagcc gcccatttca ggtacagctc gacaatttgc
    28621 gtcgggttat cgcgccatac cacgccgtga gaggtcgcta tcatatcgac tggtaagtta
    28681 aagcccagga tctcggtaat tttcggtgtt accaggcggc tgaacggcgt caggatattg
    28741 gcgtagtaac gctggcactg ctcgaaaagc tccgtctgat ccacttcatc gttgaacaga
    28801 tgctcgtcgc agtagtgctg accgaaagca tcgttactga acagcaccgc gtcgcctgtc
    28861 aggtaagtca tcatgctgtc cggccagtgc agcattggcg tttcgacaaa aatgagctgt
    28921 ttgccgttgc cgatatccag cgtgtcacca gttttcacca cattaaaatt ccactccgga
    28981 tgatgatgat gaccatttat cgagtcgata gcgttggcag tacagtaaat cggcgtatcg
    29041 ggaatttgcg ccatcagttc ggtcagcgcc ccagcgtggt cctcttctgc atggttaatc
    29101 acgatgtaat cgatatccgc cagatcgatt tcattacgca ggttctgcac aaattcgcgg
    29161 ctgaatttat ggtcgacggt gtcgatcagc acgttttttt cttcgcggat gaggtagctg
    29221 ttgtaactgc tgccgcgtag cgttttatat tccgtaccgt gaaaatcacg cacttcccag
    29281 tcacgttgac caacccaatg aatgttattt ttcaccacaa tagacatagc aacctcaatt
    29341 tattcagcgt gttctaaaaa gatgtcttgc taattgcaga ttacgtgcca gttttttatc
    29401 tgattgtttt tactctataa ataaaattat gctcattaat gagtagtcaa aatgactatc
    29461 aatcaaatgg tcgatatgac aatatctata gtcaaattga cagtgaggca aagatgagtt
    29521 tttccgttga tgtgctggcg aatatcgcca tcgaattgca gcgtgggatt ggtcatcagg
    29581 atcgttttca gcgcctgatc accacgctac gtcaggtgct ggagtgcgat gcgtctgcgt
    29641 tgctacgtta cgattcgcgg cagtttattc cgcttgccat cgacggtctg gcaaaggatg
    29701 tactcggtag acgctttgcg ctggaagggc atccacggct ggaagcgatt gcccgcgccg
    29761 gggatgtggt gcgctttccc gcagacagcg aattgcccga tccctatgac ggtttgattc
    29821 ctgggcagga gagtctgaag gttcacgcct gcgttggtct gccattgttt gccgggcaaa
    29881 acctgatcgg cgcactgacg ctcgacggga tgcagcccga tcagttcgat gttttcagcg
    29941 acgaagagct acggctgatt gctgcgctgg cggcgggagc gttaagcaat gcgttgctga
    30001 ttgaacaact ggaaagccag aatatgatgc caggcgatgc cacgccgttt gaagcggtga
    30061 aacagacgca gatgattggc ttgtcccctg gcatgacgca actgaaaaaa gagattgaga
    30121 ttgtggcggc gtccgatctc aacgtcctga tcagcggtga gacgggaacc ggtaaggagc
    30181 tggtggcgaa agcgattcat gaagcctcgc cacgggcggt gaatccgctg gtctatctca
    30241 actgtgctgc actgccggaa agtgtggcgg aaagtgagtt gttcgggcat gtgaaaggag
    30301 cgtttactgg cgctatcagt aaccgcagcg ggaagtttga aatggcggat aacggcactc
    30361 tgtttctgga tgagatcggc gagttgtcgt tggcattgca ggccaagctg ctgagggtgt
    30421 tgcagtatgg cgatattcag cgcgttggcg atgaccgtag tttgcgggtc gatgtgcgcg
    30481 tgctggcggc gactaaccgc gacttacgcg aagaggtgat ggcagggcga tttcgcgctg
    30541 acttgtttca tcgcctgagc gtgtttccac tttcggtgcc gccgctgcgt gagcggggcg
    30601 atgatgtcat tctgctggcg gggtatttct gcgagcagtg tcgtttgcgg ctggggctct
    30661 cccgcgtggt attaagtgcc ggagcgcgaa atttactgca acactatcgt tttccgggga
    30721 acgtgcgcga actggaacat gctattcatc gggcggtagt gctggcgaga gccacccgca
    30781 acggcgatga agtgattctt gaggcgcaac attttgcttt tcctgaggtg acgttgccgc
    30841 cgccagaagc ggcggcggtg cccgttgtta agcaaaacct gcgtgaagcg acagaagcgt
    30901 tccagcgtga aactattcgc caggcactgg cacaaaatca tcataactgg gctgcctgcg
    30961 cgcggatgct ggaaaccgac gtcgccaacc tgcatcggct ggcgaaacgt ctgggaatga
    31021 aggattaaat aatcccggcc tgatagaaat cctgcaggtt aattgcgccg gtgagtttgc
    31081 cgttttcatc caccaccggt gcggcagtga ttttgcgctt catcaggatc tctttggcgt
    31141 cgatggcgcg actttgcgcc tgtaacgtgg tgccgcctgt cgtcatcgct tcattaactg
    31201 gcgtggtgag tgcgccgccg cctaccagcc agcgacgtaa atcgccgtcg gtaaagacgc
    31261 cttgtacctg ttgttgagcg tcacataccg ccaccagccc cagaccggtg cggctgagtt
    31321 ccagcatcgc atccatcacg ctggcggtta acgccacctg tgggatggca tcgtcacggc
    31381 gcatcagatg atgcacttta ttcagcaagc gagcgcccag tgccccggct gggtgggagc
    31441 gggcaaaatc ttcttcatta aatccgcgcg cctgcatgac cgccatcgcc agcgcgtcac
    31501 ccatcatcag ggtattgacg gtgctggagg tcggcgcaag gtgcatcggg caggcttcgc
    31561 gttctacgga gatatccagc accgcttttg ccgccaggcc cagcggtgac gtcggtttgc
    31621 cggtcatcgc cagcagcgcg atagatttat cttccagacg cggaataatc agatccagtt
    31681 ccttcgcgcc accggagtaa gagataaaca gcatcacatc gcggctttca atcatcccca
    31741 gatcgccgtg cagtgcttct gccggatgga caaaaaaagc cgatgtgccg gtactggcaa
    31801 gtgttgcggc gattttctta ccaatgtggc ccgatttgcc aattcccgaa accaccactt
    31861 tgccttcgca gtgcaggatg atattggcgg cgcgaacaaa atcatcgccc agacgttccg
    31921 gtaaacggct tgcttcctgc aactccagca ttaacgtctg acgtcccgcg ttcagtagtg
    31981 cttcactcat tgctctctcc ggttatgatc acatcgatcc ctttctcttc cagcgcctga
    32041 cgaaacgccg gatcgatacc tgcgtcggta atcagcttat cgacgctttc aagactgcaa
    32101 actacgttgg ggcttttacg gccaaacttt gatgagtccg ccatcaaaat cacttcgcgc
    32161 gcggcattgc acattgcctt actgacggta taaacctcgt taaaggtggt tacgcccgca
    32221 ttgagatcga tgccgtcggt gcccataaac aatttatcga aactgaaatg ttcgaaggca
    32281 ttctctgcca gctgtccgtg aaatgaggcc gattttttac gaaacgttcc gcctggcatc
    32341 aggatagttt gttcgttatc cagttcagat agcgcattga cgatatgcag actgttggtc
    32401 atcaccgtga tgttattaaa gcgcgagagc aggggaacca tctgcaaaac ggtactgcca
    32461 gcatcaagaa tgatcgaatc gccatcatga ataaaactaa cggcagcttc tgctatcagc
    32521 tctttcttgt gggtgttgat gagtgtttta tgatcgatag gcggatcgga ttcctcttta
    32581 ttcaacacca ctccgccata agtacgaatg acggttcctg catgttccag aatgaccaga
    32641 tctttgcgaa tggttgtgcc tgtggtgtca aagtattgcg ccaattcttc aaccgagcat
    32701 ttaccctgct tttgcagata ctccagaatg gcggcctgac gctgacgagg tttcatgggc
    32761 gtgattacct gatttaggtt tcaaaatgat aacttcgcaa gcaagtaaca ttcacaacga
    32821 aattatccac gtttcagttt aagcgccaat gatagagcat tctgtgatag tggatcatgg
    32881 ggaaagatca catcgggctg taaatcaccc gcatgcatac cggtaattgc gggaattttt
    32941 tgcggtcgtg cgaagacggt cagtcctttc ataaacaagg tgtcgacgat gcgctgctga
    33001 tcgtccaggg cgatggcgac cacgacccgt ggtttaaagc gcccgctgga acggcccacg
    33061 ccaacccgcc cttgctggca tagtgtgtcg aaggcacggt taaaacgaga aatttgccag
    33121 ccgcctaagg ccagttgcgc acaccaggcg ataacggcga cggtgatgag tgcggatacc
    33181 ataacttttc cttaatgtag tggcggaagt cctgtatgcc tgatgagcgt agcgcatcag
    33241 gcattctatt agtctcacgg agatccttaa cggatctccg ctgttgatta caacatcacc
    33301 tgaccgccgg tgacattgat cgactgtccg gtgcagtacg acgccttagg actggcatag
    33361 agcagcagca tattcagcac atcctgataa tcgcagccgc gtttgagcgg tactttgtcg
    33421 atgtaatact gctcgacttg atccggtttg atacccagct tggtcgcgta ttggggtaac
    33481 agtgactgga acataggcga tttcagcagg ttaccgagca tcagtgaatg caccgtaatg
    33541 ccgtactccg ccagatccag cgccagtgat tgagtcagcc cgacgccacc aaatttcgct
    33601 gcgctgtagc cagagttgtg tttgctgccc actttgccgg atttcgagtt gatctgaata
    33661 atgcgaccct gaatcccgtc gcggatcatc aaacgagaaa attcacgggc acacaggaaa
    33721 taacccacca gattcacggt caaaatcgcc gagctggaag tcgctgataa aggctgcttt
    33781 tgctattccg gcgctgtaga ccagcaaatc cacgcgccca aagatttcat ctaccccacg
    33841 agagagcgcc aggacgcttt gctcgctggt ggcgtcagca ccaaaactat acgctgttcc
    33901 ttcaccatat tcggcgttaa tttcttgtgc cacatttgcg gctttgtcgc tctgaatatc
    33961 gacaaccgcg acgcgatacc cctcggcagc cagaccgtgg cacaggaacg cgcctaaggt
    34021 ttgcccacca ccgatgacaa cggcaacctg attcattttt tactccttaa cagattcaaa
    34081 cttcaaaacc gatcccggcg cgatatcgtc gggtacaggg cctgccacat ggacagtgcc
    34141 cggaaattcc gcttcgttta aaccatcgaa gcgcagggtg acatgaccca gttcgcgaag
    34201 gttgtcttcc gccacgctgc caacagcggt caccggatag cgatgctgcc cgagcgtaaa
    34261 ttgcaaaccg ggatggagtg cacctttcaa ctcgccgtgg caatgaatga agcaatactc
    34321 ttcgaggtcc gctggcgcgc cttcacgaaa ggtgatgagc atctggtcgc tgagggcgtc
    34381 agtggcactc gcgccgatac gggtgatggt ggtctgataa ataacggtca tgtttcagcc
    34441 tctattgata gataaaaccg gagacaaacc aggcgatcag tacagtcggt gcgccggtta
    34501 aaaagtggct caccagtaca gaagggacac cgacgcgaac cgtgtcctga cgggcttccg
    34561 ccagcgacaa accgaccggg atgaagtcgc aggccgcctg cgcgttgatg gcaaacagtg
    34621 ccggtaaagc cagatgcggc ggaatattgc cgagaccaat ctgcacgcca atcaatacgc
    34681 cgataacctg tgcgataact gcgcctgggc cgaggaaagg tgaaagcagc gggaaggagc
    34741 agatgagcgc cagcatgacc agacctagtg gatggctcgc cagcggagca agaccgtggg
    34801 caatccagtc accaaggcca gaagccataa tgatgccaat gagcgccgag acgaacgcca
    34861 taaacggcag aatggttttt aatacagtgt cgatggtgtc acgaccagat tgaaacaaca
    34921 cggcaacggc agaacccata cccattccca ccttcgccag taaaccatcg ctttgttcgg
    34981 tgattttctt gctggtgtca tagtcacggc ccacggaaga gggttgtggt gtcgcatcac
    35041 ccactacagt gatgttttct tcttttacgc cagagacata aatatcttcc acaatgtact
    35101 gcgccagcgg actggacttg cccgtcgagt ggatattaat ggtgggaata cgtcgtttcg
    35161 gatagatgcc gcagcgtaat gtgccgccac agtcgattac cgcgacacca atttccgcct
    35221 ccgcgggttc accttcttta aatccgtcaa tagcctgcca gccagtaagt tgtgccagtt
    35281 tgtcaacaat cgcaggccgg gtaccggcgg tgatatagac gatttttttg cccggcgtgg
    35341 cttccagctc aagcgggccg ccccagccac ccgttccttt ttcgatccga atacgcgtca
    35401 tgatgttgct cctgccaggt gaactttttg ttcaagttga atgcccatct ttttctcaaa
    35461 aatggcggtg gtcagatcgg tcacccagcc gcgaaagaag ttggtgacca gaccaaccag
    35521 cagatagctc accgccagtg ggccaagtgg caggttcagc gttgtcagac cgctggcaat
    35581 gccaagataa acaaacagtt cgccagggtt gatatggaga aagaggccat tcattgagtg
    35641 gcagctataa gaggctgccg cgtagtagct gggtttgtac ttttccggca taaagcgacc
    35701 caggcttaag gtcatcggat tgcaaaagac aaacgtgcca atgcacggta gcagcaggta
    35761 acgggaaaca gggttaccgg cgcaacgttg agcaaaacgt tcaatacgat gctgaccgat
    35821 aaaattaatc agtgcgttca tgataaccag caggctaatc aacagcggaa gaatgccggt
    35881 caccatcccg gtaaacacct ctccgccctt ttggaacagc ccgataaacc actctgcacc
    35941 atgagtaatg gtttctatca ttgttctctc cttcaggatt tattgtttta ttaccaaacg
    36001 gcaacctaat ctaatcagat tgaaagattt gaaagtgtta ttttgatcgc aaaatgaaag
    36061 ataaatattt taatttgaaa gtttgaataa aaggatagcg aggggaatga gttgagttat
    36121 gtaaagtccg tatcgggcag tgactaccgc ttccttgtgc ggggcgtgat gctttaccat
    36181 acttgcccct ggttgaatct gttaaatgga cccctcatgt tcaagcgtcg ttatgtaaca
    36241 ttgcttcccc tttttgtgtt gcttgccgcc tgtagcagca agccaaaacc tactgagact
    36301 gaaacgacca ccggaacgcc gtctggcggc ttcctgcttg agcctcagca caatgtgatg
    36361 cagatgggcg gcgatttcgc taataacccg aatgcccagc agttcatcga cagaatggtg
    36421 aacaaacacg gtttcgatcg tcagcagttg caggaaattc tctcccaggc gaagcgtctg
    36481 gattcggtac tgcggctgat ggataaccag gcaccaacca catcggtgaa accgccatca
    36541 ggtccgaacg gcgcatggct gcgttatcgc aaaaaattta ttacgccgga caacgtgcag
    36601 aacggtgtgg ttttctggaa tcagtatgaa gatgcgttga atcgcgcgtg gcaggtgtat
    36661 ggcgtaccgc cggaaattat cgtcgggatt atcggcgttg aaacccgctg ggggcgcgtg
    36721 atggggaaaa ctcgcatcct cgatgcgctg gcaacgctgt catttaacta cccacgccgc
    36781 gcggagtatt tctctggcga gctggaaacc ttcctgttga tggcgcgcga cgagcaggac
    36841 gatccgctca atctgaaagg ttcctttgcc ggggcgatgg gctacggaca gtttatgccg
    36901 tcgtcttaca aacaatatgc ggtagatttc agcggcgacg ggcatatcaa cctgtgggat
    36961 ccggttgatg cgatcggtag tgtggcgaac tatttcaaag cgcacggctg ggtgaaaggc
    37021 gatcaggtcg cggtaatggc aaacggtcag gctccaggct tgccaaatgg cttcaaaacc
    37081 aggtacagca tttcgcagct tgccacggca ggtttaacgc cacagcagcc gctgggcaac
    37141 catcaacaag ccagcctgct gcgtctggat gtcggtaccg gttaccagta ctggtacggt
    37201 ctgccgaact tctacaccat tacccgttat aaccacagca cccattacgc aatggcggtc
    37261 tggcagttag gacaagccgt ggcgctggcg cgcgtgcagt aactatttgc aagagggggg
    37321 cgtgagcttc cctctggcta atcagctttt ttctgaatcc tcctcgtaaa attgcaacgc
    37381 caacaccatc ttcctgacga aagtgctatc ttgtccggca taaatttttg actgactgag
    37441 gttgtgatga ctgacagtga actgatgcag ttaagcgaac aggttgggca ggcgctgaaa
    37501 gcccgtggcg caaccgtaac aactgccgag tcttgtaccg gtggttgggt agcgaaagtg
    37561 attaccgata ttgccggtag ctccgcctgg tttgaacgcg gatttgtcac ctacagtaac
    37621 gaagccaaag cgcagatgat cggcgtacgc gaagagacgc tggcgcagca tggcgcggtg
    37681 agtgaacccg tcgtggtgga aatggcgata ggtgcactga aagcggctcg tgctgattat
    37741 gccgtgtcta ttagtggtat cgccgggccg gatggcggca gtgaagagaa gcctgtcggc
    37801 accgtctggt ttgcttttgc cactgcccgc ggtgaaggca ttacccggcg ggaatgcttc
    37861 agcggcgacc gtgatgcggt gcgtcgtcag gctactgcgt atgcattgca gaccttgtgg
    37921 caacaatttc tacaaaacac ttgatactgt atgagtatac agtataattg cttcaacaga
    37981 acatattgac tatccggtat tacccggcat gacaggagta aaaatggcta tcgacgaaaa
    38041 caaacagaaa gcgttggcgg cagcactggg ccagattgag aaacaatttg gtaaaggctc
    38101 catcatgcgc ctgggtgaag accgttccat ggatgtggaa accatctcta ccggttcgct
    38161 ttcactggat atcgcgcttg gggcaggtgg tctgccgatg ggccgtatcg tcgaaatcta
    38221 cggaccggaa tcttccggta aaaccacgct gacgctgcag gtgatcgccg cagcgcagcg
    38281 tgaaggtaaa acctgtgcgt ttatcgatgc tgaacacgcg ctggacccaa tctacgcacg
    38341 taaactgggc gtcgatatcg ataacctgct gtgctcccag ccggacaccg gcgagcaggc
    38401 actggaaatc tgtgacgccc tggcgcgttc tggcgcagta gacgttatcg tcgttgactc
    38461 cgtggcggca ctgacgccga aagcggaaat cgaaggcgaa atcggcgact ctcacatggg
    38521 ccttgcggca cgtatgatga gccaggcgat gcgtaagctg gcgggtaacc tgaagcagtc
    38581 caacacgctg ctgatcttca tcaaccagat ccgtatgaaa attggtgtga tgttcggtaa
    38641 cccggaaacc actaccggtg gtaacgcgct gaaattctac gcctctgttc gtctcgacat
    38701 ccgtcgtatc ggcgcggtga aagagggcga aaacgtggtg ggtagcgaaa cccgcgtgaa
    38761 agtggtgaag aacaaaatcg ctgcaccgtt taaacaggct gaatttcaga tcctctacgg
    38821 cgaaggtatc aacttctacg gcgaactggt tgacctgggc gtaaaagaga agctgatcga
    38881 gaaagcaggc gcgtggtaca gctacaaagg tgagaagatc ggtcagggta aagcgaatgc
    38941 aactgcctgg ctgaaagata acccggaaac cgcgaaagag atcgagaaga aagtacgtga
    39001 gttgctgctg agcaacccga actcaacgcc ggatttctct gtagatgaca gcgaaggcgt
    39061 agcagaaact aacgaagatt tttaatcgtt cgttattgat gataatagat aatgtgtgca
    39121 cggacggtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccaacactg
    39181 gcgtgaaaaa ttactctcac ctcgatcctc taacgaattg gctgcacact cattgactat
    39241 taaatctgtt tatcagcaaa acctgggtcg catctgcggc ccttttgctt ttttaagttg
    39301 taaggatatg ccatgacaga atcaacatcc cgtcgcccgg catatgctcg cctgttggat
    39361 cgtgcggtac gcattctggc ggtgcgcgat cacagtgagc aagaactgcg acgtaaactc
    39421 gcggcaccga ttatgggcaa aaatggccca gaagagattg atgctacggc agaagattac
    39481 gagcgcgtta ttgcctggtg ccatgaacat ggctatctcg atgacagccg atttgttgcg
    39541 cgctttatcg ccagccgtag ccgcaaaggt tatggacctg cacgtattcg tcaggaactg
    39601 aatcagaaag gtatttcccg cgaagcgaca gaaaaagcga tgcgtgaatg tgacatcgac
    39661 tggtgcgcac tggtgcgcga tcaggcgacg cgaaaatatg gtgaaccttt gccaactgtc
    39721 ttttcagaaa aagttaagat ccagcgtttt ctgctctatc gtggctatct gatggaagat
    39781 atccaggata tttggctaaa ttttgccgac tgaacgcata cggtatttta cttcccactc
    39841 aagaaaactt atcttattcc cacttttcag ttaccagccc ggcggttaag acacgctgga
    39901 gctggtggcg atatttcgtt agcttgattt caggataatt atgagcaaga gcaccgctga
    39961 gatccgtcag gcgtttctcg actttttcca tagtaaggga catcaggtag ttgccagcag
    40021 ctccctggta ccccataacg acccaacttt gttgtttacc aacgccggga tgaaccagtt
    40081 caaggatgtg ttccttggac tcgacaagcg taattattcc cgcgctacca cttcccaacg
    40141 ctgcgtgcgt gcgggtggta aacacaacga cctggaaaat gtcggttaca ccgcgcgtca
    40201 ccataccttc ttcgaaatgc tgggcaactt cagcttcggc gactatttca aacacgatgc
    40261 cattcagttt gcatgggaac tgctgaccag cgaaaaatgg tttgccctgc cgaaagagcg
    40321 tctgtgggtt accgtctatg aaagcgacga cgaagcctac gaaatctggg aaaaagaagt
    40381 agggatcccg cgcgaacgta ttattcgcat cggcgataac aaaggtgcgc catacgcatc
    40441 tgacaacttc tggcagatgg gtgacactgg tccgtgcggc ccgtgcaccg aaatcttcta
    40501 cgatcacggc gaccacattt ggggtggccc tccgggaagt ccggaagaag acggcgaccg
    40561 ctacattgag atctggaaca tcgtcttcat gcagttcaac cgccaggcag atggcacgat
    40621 ggaaccgctg ccgaagccgt ctgtagatac cggtatgggt ctggagcgta ttgctgcggt
    40681 gctgcaacac gttaactcta actatgacat cgacctgttc cgcacgttga tccaggcggt
    40741 agcgaaagtc actggcgcga ccgatctgag caataaatcg ctgcgcgtaa tcgctgacca
    40801 cattcgttct tgtgcgttcc tgatcgcgga tggcgtaatg ccgtccaatg aaaaccgtgg
    40861 ttatgtactg cgtcgtatca ttcgtcgcgc agtgcgtcac ggcaatatgc tcggcgcgaa
    40921 agaaaccttc ttctacaaac tggttggtcc gctgatcgac gttatgggct ctgcgggtga
    40981 agacctgaaa cgccagcagg cgcaggttga gcaggtgctg aagactgaag aagagcagtt
    41041 tgcccgtact ctggagcgcg gtctggcgtt gctggatgaa gagctggcaa aactttctgg
    41101 tgatacgctg gatggtgaaa cggctttccg tctgtacgac acctatggct tcccggttga
    41161 cctgacggct gatgtttgtc gtgagcgcaa catcaaagtt gacgaagctg gttttgaagc
    41221 agcaatggaa gagcagcgtc gtcgcgcgcg cgaagccagc ggctttggtg ccgattacaa
    41281 cgcaatgatc cgtgttgata gtgcatctga atttaaaggc tatgaccatc tggaactgaa
    41341 cggcaaagtg accgcgctgt ttgttgatgg taaagcggtt gatgccatca atgcaggcca
    41401 ggaagctgtg gtcgtgctgg atcaaacgcc attctatgcg gaatccggcg gtcaggttgg
    41461 tgataaaggc gaactgaaag gcgctaactt ctccttcgcg gtggaagata cgcagaaata
    41521 cggccaggcg attggtcaca tcggtaaact tgctgcgggt tctctgaaag tgggcgacgc
    41581 tgtgcaggct gatgttgatg aggctcgtcg cgcccgtatt cgtctgaatc actccgcaac
    41641 gcacctgatg cacgctgcgc tgcgccaggt tctggggact catgtatcgc agaaaggttc
    41701 actggttaac gacaaggtgc tgcgcttcga cttctcacac aacgaagcga tgaaaccgga
    41761 agagattcgt gcggtcgaag acctggtgaa cgcacagatt cgtcgcaatt tgccgatcga
    41821 aaccaacatc atggatctcg aagcggcgaa agcgaaaggt gcgatggcgc tgttcggcga
    41881 gaagtatgat gagcgtgtac gcgtgctgag catgggcgat ttctccaccg agctgtgtgg
    41941 cggtactcac gccagccgca ctggtgatat tggtctgttc cgcatcatct ctgaatcggg
    42001 tactgctgca ggcgttcgtc gtatcgaagc ggtaaccgga gaaggcgcta tcaccaccgt
    42061 tcatgcagac agcgatcgct taagcgaagt cgcgcatctg ctgaaaggcg atagcaataa
    42121 tctggcggat aaagtgcgct cagtactgga acgtacgcgt cagttagaaa aagaactaca
    42181 acagcttaaa gaacaagctg ccgcacagga gagcgcaaat ctttccagta aggcaattga
    42241 tgttaatggt gttaagctgt tggttagcga gcttagcggt gttgagccga aaatgttgcg
    42301 taccatggtt gacgatttaa aaaatcagct ggggtcgaca attatcgtgc tggcaacggt
    42361 agccgaaggt aaggtttctc tgattgcagg cgtatctaag gacgtcacag atcgtgtgaa
    42421 agcaggggaa ctgattggta tggtcgctca gcaggtgggc ggcaagggtg gtggacgtcc
    42481 tgacatggcg caagccggtg gtacggatgc tgcggcctta cctgcagcgt tagccagtgt
    42541 gaaaggctgg gtcagcgcga aattgcaata atataagcgt caggcaatgc cgtggactcg
    42601 cttcacggca ttcgcattaa cgctatcgac aacgataaag tcaggttgaa gttgtgtata
    42661 tcggctaaac ttaggtttaa cagaatgtaa tgccatgact gcttacatgt aatgtgtttg
    42721 tcattgctta ctttttggcg ttatatgatg gataatgccg ggatacagag agacccgact
    42781 cttttaatct ttcaaggagc aaagaatgct gattctgact cgtcgagttg gtgagaccct
    42841 catgattggg gatgaggtca ccgtgacagt tttaggggta aagggcaacc aggtacgtat
    42901 tggcgtaaat gccccgaagg aagtttctgt tcaccgtgaa gagatctacc agcgtatcca
    42961 ggctgaaaaa tcccagcagt ccagttacta atctttccgc gtctcatctt tatcggtgag
    43021 acgcaccctc aaaatttctc cttcactcta tagtcttttc gctttactcc cgttcattca
    43081 acttaagtct ccattttttt gcattactgc tatctgccag acctccgttt ttctgtagat
    43141 aaaacactct ttttgacgtt tttacagact aattgagcgt gaagtgtgca aacgataaaa
    43201 gtgtgggaaa aattgtttga cttataagtc tcagaaagta atatgtgcgc cacgcagcga
    43261 cgatgagcga taaacaagtt cttcgaagca ctcgtaagag gcgtgtggtg aggtggccga
    43321 gaggctgaag gcgctcccct gctaagggag tatgcggtca aaagctgcat ccggggttcg
    43381 aatccccgcc tcaccgccat ttgcatccgt agctcagctg gatagagtac tcggctacga
    43441 accgagcggt cggaggttcg aatcctcccg gatgcaccat ctcttacttg atatggcttt
    43501 agtagcggta tcaatatcag cagtaaaata aattttcccg atgcatccgt agctcagctg
    43561 gatagagtac tcggctacga accgagcggt cggaggttcg aatcctcccg gatgcaccac
    43621 ctcttacttg atatggcttt agtagcggta tcaatatcag cagtaaaata aattttcccg
    43681 atgcatccgt agctcagctg gatagagtac tcggctacga accgagcggt cggaggttcg
    43741 aatcctcccg gatgcaccat attctccgaa atcttcagca atgaaggtat cgaagaggta
    43801 gcggaattaa ccgcaggcac cagggatgat aacgttgctt tagcaacggc ccgaagggcg
    43861 agccgcaagg cgagtaatcc tcccggatgc accatctctt aattgatatg gctttagtag
    43921 cggtatcaat atcagcagta gaataagttt tcccgatgca tccgtagctc agctggatag
    43981 agtactcggc tacgaaccga gcggtcggag gttcgaatcc tcccggatgc accatattct
    44041 ccgtaacctt cagcaatgaa ggtatcgaag agatagcggt attaaccgca ggcaccagga
    44101 aggataacgt tgcgccagca acggcccgaa gggcgaacgt cagtgagtca tcctcccgga
    44161 tgcaccatct tctccaaaaa atttccttca aaaatatctc atttctaaat aactccttta
    44221 tccatttcct gaattttctt ctccttcgcc atcagcgaca aaatcaagca attacgctaa
    44281 agtagcgtca tatttttcga cttgcgagaa gaccatgtac gagcgttatg caggtttaat
    44341 ttttgatatg gatggcacaa tcctggatac ggagcctacg caccgtaaag cgtggcgcga
    44401 agtattaggg cactacggtc ttcagtacga tattcaggcg atgattgcgc ttaatggatc
    44461 gcccacctgg cgtattgctc aggcaattat tgagctgaat caggccgatc tcgacccgca
    44521 tgcgttagcg cgtgaaaaaa cagaagcagt aagaagtatg ctgctggata gcgtcgaacc
    44581 gcttcctctt gttgatgtgg tgaaaagttg gcatggtcgt cgcccaatgg ctgtaggaac
    44641 ggggagtgaa agcgccatcg ctgaggcatt gctggcgcac ctgggattac gccattattt
    44701 tgacgccgtc gtcgctgccg atcacgtcaa acaccataaa cccgcgccag acacattttt
    44761 gttgtgcgcg cagcgtatgg gcgtgcaacc gacgcagtgt gtggtctttg aagatgccga
    44821 tttcggtatt caggcggccc gtgccgcagg tatggacgct gtggacgttc gcttgctgtg
    44881 agtgaagcgt tatcgctttt ctcgttgttt gccagtagtt ttctcagcgc tacactatta
    44941 cccggcaact ccgaagtcgt gctggtggca atgttgcttt ccgggatcag tcatccctgg
    45001 gttttagttt taacagcaac aatgggtaat agccttggag ggttaactaa cgttatcctt
    45061 gggcgtttct ttccattgcg taaaacatcg cgctggcaag agaaagctat cggctggctg
    45121 aaacgctatg gtgcagtcac gctattatta agctggatgc ccgtggttgg cgatttactg
    45181 tgtctgttag cgggatggat gcgcatctcg tggggaccgg taatcttttt tttgtgcctt
    45241 ggtaaagcgt tacgctatgt tgcagttgca gcagcgaccg ttcagggcat gatgtggtgg
    45301 cactaattgt aggcctgcac atatggtcac cattacagtt atgctaatta aaacgatttt
    45361 gacaggcggg aggtcaattt gatcccggac gtatcacagg cgctggcctg gctggaaaaa
    45421 catcctcagg cgttaaaggg gatacagcgt gggctggagc gcgaaacttt gcgtgttaat
    45481 gctgatggca cactggcaac aacaggtcat cctgaagcat taggttccgc actgacgcac
    45541 aaatggatta ctaccgattt tgcggaagca ttgctggaat tcattacacc agtggatggt
    45601 gatattgaac atatgctgac ctttatgcgc gatctgcatc gttatacggc gcgcaatatg
    45661 ggcgatgagc ggatgtggcc gttaagtatg ccatgctaca tcgcagaagg tcaggacatc
    45721 gaactggcac agtacggcac ttctaacacc ggacgcttta aaacgctgta tcgtgaaggg
    45781 ctgaaaaatc gctacggcgc gctgatgcaa accatttccg gcgtgcacta caatttctct
    45841 ttgccaatgg cattctggca agcgaagtgc ggtgatatct cgggcgctga tgccaaagag
    45901 aaaatttctg cgggctattt ccgcgttatc cgcaattact atcgtttcgg ttgggtcatt
    45961 ccttatctgt ttggtgcatc tccggcgatt tgttcttctt tcctgcaagg aaaaccaacg
    46021 tcgctgccgt ttgagaaaac cgagtgcggt atgtattacc tgccgtatgc gacctctctt
    46081 cgtttgagcg atctcggcta taccaataaa tcgcaaagca atcttggtat taccttcaac
    46141 gatctttacg agtacgtagc gggccttaaa caggcaatca aaacgccatc ggaagagtac
    46201 gcgaagattg gtattgagaa agacggtaag aggctgcaaa tcaatagcaa cgtgctgcag
    46261 attgaaaacg aactgtatgc gccgattcgt ccaaaacgcg ttacccgcag cggcgagtca
    46321 ccttctgatg cgctgttacg tggcggcatt gaatatattg aagtgcgttc gctggacatc
    46381 aacccgttct cgccgattgg tgtagatgaa cagcaggtgc gattcctcga cctgtttatg
    46441 gtctggtgtg cgctggctga tgcaccggaa atgagcagta gcgaacttgc ctgtacgcgc
    46501 gttaactgga accgggtgat ccttgaaggt cgcaaaccgg gcctgacgct gggtatcggc
    46561 tgcgaaaccg cgcagttccc gttactgcag gtgggtaaag atctgttccg cgatctgaaa
    46621 cgcgtcgcgc aaacgctgga tagcattaac ggcggcgacg cgtatcagaa agtgtgtgat
    46681 gaactggttg cctgcttcga taatcccgat ctgactttct ctgcccgtat cttaaggtct
    46741 atgattgata ctggtattgg cggaacaggc aaagcgtttg cagaagccta ccgtaatctg
    46801 ctgcgtgaag agccgctgga aattctgcgc gaagaggatt ttgtagccga gcgcgaggca
    46861 tctgaacgcc gtcagcagga aatggaaacc gctgataccg aaccgtttgc ggtgtggctg
    46921 gaaaaacaca cctgacagaa aagaaaaagg ccactcgtga gtggccaaaa tttcatctct
    46981 gaattcaggg atgatgataa caaatgcgcg tctttcatat actcagactc gcctgggaag
    47041 aaagagttca gaaaattttt aaaaaaatta ccggaggtgg ctaaatgccg ttgttagata
    47101 gcttcacagt cgatcatacc cggatggaag cgcctgcagt tcgggtggcg aaaacaatga
    47161 acaccccgca tggcgacgca atcaccgtgt tcgatctgcg cttctgcgtg ccgaacaaag
    47221 aagtgatgcc agaaagaggg atccataccc tggagcacct gtttgctggt tttatgcgta
    47281 accatcttaa cggtaatggt gtagagatta tcgatatctc gccaatgggc tgccgcaccg
    47341 gtttttatat gagtctgatt ggtacgccag atgagcagcg tgttgctgat gcctggaaag
    47401 cggcaatgga agacgtgctg aaagtgcagg atcagaatca gatcccggaa ctgaacgtct
    47461 accagtgtgg cacttaccag atgcactcgt tgcaggaagc gcaggatatt gcgcgtagca
    47521 ttctggaacg tgacgtacgc atcaacagca acgaagaact ggcactgccg aaagagaagt
    47581 tgcaggaact gcacatctag tcagtaaact atcttcacaa ttaattgaaa aaagccagtt
    47641 caaatgaact ggcttagttg tacttagtgc gcaccgccgc caccgccacc tgcgccaaat
    47701 ggcggtttag caaaccacac cagccccagc agtacgagga atatcccggc tgacatccag
    47761 aagatctcat tggcggaaat aatcagcccc tgattggtga tctgctgggc aatccagcct
    47821 gatgcctgct gttgcgtcat cccaagccct tccagttgac tgtacatcgc ctgggcattc
    47881 gggttgaacg ggtttaccga ctcagtcaac tgcgcatggt gcatcgactc gcggttggtc
    47941 cacatggtcg tggttatcga cgtgccgata gaccccgcca gcgttcgcgt aaagttagag
    48001 aggctcgatg ccgccgccag tcgttccggt ggcaaaccag acagcgtaat ggtggtcagc
    48061 ggcataaaga agcaggccac cgcaaacccc tggataaact gcggccaggc cgacgcgcca
    48121 aaatccatac ctggttcaaa ggtataggca cgccagtaga agcagacggc atacataata
    48181 aagctgaagg ttaccagccg ccgcatatcc agtttatgcg cgaagcggcc gataatcggc
    48241 gacaggatca ccggaataat ccctaccggc gcagaggcca aacctgccca ggtcgccgtg
    48301 taaccgtaga cctcctgcaa caactgcggc agcagaacaa tagcgccgaa gtagagcata
    48361 tacgcgaggc tgatacacaa gcagccgatg gtgaagttgc gcgacttaaa caacgacaga
    48421 tcgactatcg ggttatcgtc ggtcagctcc cagacaatca ggaagcagat agccaccacc
    48481 gccaccacgg taaggatgat aatttcctgt gatgaaaacc agtccagctc tttaccgcgg
    48541 tcgagcataa tctgcaggct gccgatacca ataaccagca gtgccagccc cacggcatca
    48601 atccgccgcc gtttggtgcg ggtttcacgt ccgcgcagag tttgcagtgt catcaacacc
    48661 accgccacgc caatcggcac gttgatgaag aatatccagc cccagtggta attatcgctg
    48721 atataaccgc cgaggatcgg gccgcaaatt ggcgcgacaa tcaccgtcat cgaccacaac
    48781 gccagcgcga tcgagcgttt ggctggcggg tagttattca gcaatagact ttgcgaaagc
    48841 gggatcaacg gcccggcgac aatcccctga atcacgcgga agaagatcag catattcagg
    48901 ctgctggaga caccacacgc ccacgacgca atagcaaagg cgatggtgga ccaaaggaac
    48961 agtttcactt ccccgacgcg ctttgccagc cagccggtaa gcgggatcga gatggcattc
    49021 gccaccccga aagaagtgat tacccacgtt ccctggctga gcgatgagcc cagattcccg
    49081 gcgatagtgg ggatcgccac gttagcaatg gtggagtcca gcacctgcat gaatgtcgcc
    49141 agtgacagcg caatcgtcat aatgaccagt tgcgcgcctt ccagcggttt ttgctgttgc
    49201 atcacacgca cctctggatt agccagcgtt agcttttacg atatcgtcga tcagtttatt
    49261 gacaggtgcc aggctgattt cacgcgcggt acttaccgcg accggtgttg aacgtacttt
    49321 atttgccagt acctgaccgt cacggttagt ggtattgacg ctcaccagtg tggacaaacc
    49381 aatgcgcagc gggtattgct ccagctgttt ctggtccagt tcgatacgca caggtagacg
    49441 ctgaacgact ttgatccagt taccggtcgc attttgcgcc ggaagcagtg agaatgcgct
    49501 acctgtgccc atatccagac caaccacttt accggtgtat ttcacatcat cgccgtaaat
    49561 atccgtggtg atagtgaccg gctgaccgat acgcatattg gcaatctgcg tctctttaaa
    49621 gttggcatcc acccacatat tggtggctgg aacgaccgcc atcagcggcg tcgttgggct
    49681 aatttgcgcc ccaggctgta ccgcacggcg ggagacataa ccggtcatcg gactgacaat
    49741 acgggtacgc tccagcgcca gccaggcgtt acgtacttcg gtggcagcct gttgcacggc
    49801 aggctgatct tccagtttag tccccagaat catcgcctga ttggcattgt attgttgaat
    49861 agcgacgtcc agttgcgcct gggcactggt gacggcgtcg cgggcgtgtt gcagctcttc
    49921 gcgaccaatc aggttggcat tgcccagcgg cacacggcgg ttgtagtcgc tttgtgcttt
    49981 tgcgagggcg attttctgca cctcaatatt cgcctgcaac tgtttgctgt taatcatcag
    50041 ctggtgggtt tggcgaacgc tggaagccag tgcagttttg gctttttcaa acgcctggcg
    50101 agcatctgtc gggtcgagag tgaccagcac gtcgccttct tttacaaaat cggtgttatc
    50161 ggcccagact ttcgtcacgc tgccagacac ctgagacata atttgaatct gattccctgc
    50221 cacgtatgcg tcatcggttt cttcgaaatg acgcagtacc aaaaaccaat aaatccctat
    50281 cgctacggca ataattataa agagcaaggt gagaaggagg agcagacgct tacgtttgcc
    50341 gctcttcttt accggttgct gcggggtttg agtctccgca tttgcgctca tattgttctc
    50401 cacgatcttc ttaactcatc ggctgagccg acctgcttgc caaaaaggcc agcatgttgc
    50461 gatgctggcc ggtcattttt tcttttataa atctggattt ttgagcgaga tgacgcgtta
    50521 gctcatcgct tcgagaacca caccgtcttg ttccatctgg tcgagacggg agagcaattt
    50581 gcgggtgatt tgctcgagct gatctttttc tgttgtgctg agcgcggacc agagttgatg
    50641 caggcagtta tgctgcggtg gtaaaacctc gcgcaaaaac tcgtgacctt tttccgttaa
    50701 ttgcagatgc aggcagcggc gatcgttatc gctttcacga cgttcgatcc aaccgcgttt
    50761 ttccagttca tcggcaatac gcgtcgcgtt ggtacgggat gatccaagag cacaacttaa
    50821 ttcagaaggc tgaatactgt ggttttcctg agactccagc gtaatcaacg ccataaacaa
    50881 cgtctcgtta atcccctgag ccttcagcat tttattgcgg ttctccagca gcttgctttg
    50941 catgtgcatg caaagacgag tcagaaggat ctcctgataa ggaaaatctt cgtggcggct
    51001 ggcgcgaaat tttagcattt gttcaatggg cgtaaacgaa ctatccattt gggtatgacc
    51061 tcattaattt cagccgatat agtaacgaca gtgacaaata aagtaaatgt attgttttag
    51121 aaaaatgatt cttgtgggtt atataatcgc catcactttc caggcgagcc cataggccag
    51181 cgcactaagc agtgttggga tgataatgct gcgtgtttta tagaaactgg cacccagtac
    51241 cgcgaagccg accagcgtgg gcacgaaacg gcgtgtatcg tgcatcactt ctggtgcggt
    51301 agagacaacc agcagagcgc atatcgaggc gatgccaatg gtgtcgagca aaatacctac
    51361 cgcgccacgt ttggttgggc gggcattacc cacacgcagg cgcagcggca aatagcggaa
    51421 gcaataattc gccgcgccaa ccagtaaccc aggcagcaga acctcatagc tcatcgggcg
    51481 ctccttgcca gaatgcttgg attaacgcag tgaggcagcc acagacaatg cctgccagaa
    51541 tggcgacggg aatagaaaat agcgttacgc ctgcaagggc accaactaac gctgcggtaa
    51601 cgcaaagaga ttgtttgcgc tggaaagagg cgagcaggaa actcataaag agtgccggaa
    51661 gcataaaacc taatgctgct tcaacggcgg gataaccttg cagcaagccg ctgccagaga
    51721 atgcccctat taccgtacca aatacccacg atgaccatga actgaaggca atgccgatca
    51781 tccagttctc gctccagcgg cgattattgc gtaccagttt tgctgttgcg gcggcaaaaa
    51841 cctcatccgt caggccaaac gcccacaggg cggttttcga tttttgcaga cgctgaataa
    51901 tacggctacg cagtgacggg ccatacaaca catggcgaac atccattgcc atgacggtca
    51961 gtgcagcaac ccacaaacta ctcccggctg ccagcatcgc ggtaatgacg aactggctcg
    52021 cgcctgcata aatgatgcag gagaaaaaaa cgctttcgag aggagagaat cccagacggg
    52081 tcgcattcag accgaacgca aaggccaccg gaatataact aataacaatc ggtaaactgt
    52141 ctttgcatcc ttccatgaag gtcgccgaac caggagcagg ctgtggagta gggctttcca
    52201 tagagtgtac gcttaacgat tgttaatatt ataaatagac tgaatgaata tcttaacctt
    52261 atcagactga tgggcttctt aacaccctta taagtgtaaa gccacgaaaa cggttgctga
    52321 ttgcttaatt tgcggcttcc tgacgatgaa aacctcgcca ccagaccagt aagttaacta
    52381 gggcaatcgt cgcgccagcc agacaaacgc cagcccaacc gccatgttgc caggctgagg
    52441 ctgaaattag cgaaccggcg gcaccgccaa taaagtagct ggtcatgtaa cctgcggtca
    52501 ggcgattacg cgcatcaggg tgtattcgat aaattaccgt ctggttagtg atatgcacgc
    52561 cctgtacggt gagatccagc accaggatgc cgataatcag cgccagtacg gaagtgtgac
    52621 caaaccagat cgccagccat gaaagtaata gcagcagcag accgaaagtt gtggtgtggt
    52681 gcgatttacc cttatcggca aaaccgcccg ccggacgagc gcccaacgct ccggcagctc
    52741 ccgcaagtcc aaacagacca atgacaccat cgctgtagtt aaaaggtgga gcggcaagca
    52801 aaaaggccat tgaggtccag agaatgctga agttggcaaa ggtcaggcag cccagcaatg
    52861 cgcgggtgcg gaggatctta tcgctgataa acatgctgaa aacggaacct aacaactgtg
    52921 ggtagttgag gtgggtttct gatttcattt gtggcagacc acgccataat gctagcgcca
    52981 tcagtgccat taacaccgag gcaacccaaa agacggtgcg ccagccgccg aggtttgcca
    53041 gtaacccggc aacggttcgt gccagcaaga tccccaacag cagaccgctc ataatggtgc
    53101 caaccacttt gccgcgttta tccggtgaag ccagcgtcgc tgccagcgga accagaattt
    53161 gtgccacgac tgagaataaa ccggttaatg ccgtaccgag gatcatcatc gccagcgact
    53221 gactgctggc agtgattaac atgccgcctg ccgccagtaa ggtcatcgag acaatcaggc
    53281 ggcggcgttc aaacatatca ccgaggggaa caagaaacag cagacctgct gcatagccca
    53341 actgcgcggc ggtaacaata aagcctgccg aactggcgga aagggaaaag ttacgcgcga
    53401 tggtgtcgag caatggctgg gcgtaatagt tactggctac cgcctgaccg gtggcgatag
    53461 acatcagcac gatcagcgcc gggctaagct catgattagg tttagtcatt atttttgttc
    53521 atcaggtgat gattaagctg gaaatcaata atagcggatg ttgatgaatg ggggcgaaag
    53581 atagcttgtt gaaatgtgcg atgtggtttg taggcatgat aagacgcgcc agcgtcgcat
    53641 caggcattgt gcacagaacg gcggatgcgg cgaaaacgcc ttatccgcca tacggaatga
    53701 gtgtattttg taggcatgat aagacgcgcc agcgtcgcat caggcattgt gcacagaatg
    53761 gcggatgagg caaaaacgac ttatccgccc gaataaaaat tacttctgcg ctgccagcgc
    53821 ctcattcacc cagccatcga actgctgctg gtgggctttg atccaaccat caacatggcc
    53881 ctgaatatcg ccttctgagg ctttgccgtc atgcataatg gcgttctggg cgttaatatc
    53941 tgccactggc aactgcataa tggcaaaaag tttcgctgct gccgggtttt tctcggccca
    54001 ggctttgttg gcaacgatat gcatggtgct gaccgggaag ccataattcg caccattcgg
    54061 cagtttggta tcggcgtttt tatcgcccgg cagtgcggag aacggcacct gcaaccagac
    54121 cacatctttc cctggcttca gctcattact cacccagtac ggcgtccagg tgtaataaaa
    54181 caccggtttg ccctctttgt agcgactgat ggtgtcggcc atcatcgctg cgtagttccc
    54241 ctgattatgc gtcacggtgt tggtcagttc atacgcggca agctggtggt tgatcgcacc
    54301 ttcgcagccc cagcctgggt tacagccggt taaatccgct ttaccgtcgc cgttggtatc
    54361 gaacagtttg gcgatcttcg gatctttcag ttgtgcgatg ttggtgattt tgtactggtc
    54421 ggcggttttc ttatcgatca ggtaaccctg tgccgcgccg ttaacgaata tcccttcaca
    54481 ataaaatttc ttatcgccac cggcagcttc gtacatgttg tcatgcagcg gcgtccagtt
    54541 cacggcggtg aaggttgcat cgccggaagc aagtgaggtg tagccaacgt tgtaatccac
    54601 ttcgctgggt ttattgacgg tataacctaa tttctccagt gcacgactga ccagcagcgt
    54661 ctggaaggtt tcttcagtga tggtgctctg aactggatta acagtaatgc ctttgcccgg
    54721 cagatcggct gcaaaagttt gtgtagagat aagcgtggca aacgctgtcg caaaaagtac
    54781 gctatgtcgc attgttattc ctttttttgg cagggtgata atgcccggcg ttaccgggca
    54841 agtgcagagt tacttaatga atgggcgggt cagcagaccg acagggccag tggtgtacca
    54901 gcgacggttg ccgcgactgc gtgagtcgcg cccaacggcc tgcgtcaggc ggtcgaggat
    54961 aatggcgagg atcacaatcc cgacgccgcc aacggtggca aggcccatat ccagacgacc
    55021 gataccgcga agtaccatct gacccagccc gccgacggca atcatcgagg cgatgaccac
    55081 catagaaaga gccagcatca gcgtctggtt aacgcccgcc ataatggtcg gcatcgccag
    55141 cggtaactga actttgaaca gcatctggcg cgggctggca ccgaatgagc gcgaggcttc
    55201 aatcagatcc gccggaacct ggttaatccc cagaatcgtc agacggataa tcggcggcag
    55261 agcaaagatg atcgtcacca ctacgcccgg cacgttaccg ataccaaaca gcatgacgat
    55321 tggtaccaga taaacaaacg ctggcgtggt ctgcatggca tcaagcagtg gacgaataat
    55381 tttcgccgct cgcggacttc tcgccagcca tatccccaac ggcaaaccga tgacgataca
    55441 gaacagcagg gcggttaaca ccagcgccag agtcaccatt gcctgcgacc aggcaccgat
    55501 tgcgccgatg gcaatcagcg aaaccagcgt cgccacgccc attccgaccc cggaaatctg
    55561 ccaggcgatg agagcgaaaa cgataatcgc cactggcgct ggcataccca gcagcaattg
    55621 ctggaaactg ttgaggatat aatcaaccgg aacgcgcacg ccctggaaga cgggacggaa
    55681 atgggtaaca acccagtcga tcccttcagt gacccaactg tcgagcggga tcagcgtttt
    55741 atggaacgga tcgagaatat taaaatgctc gacgtttggc gcaggcgtac tggtcagcca
    55801 gtcagcacca ccgccgtcag tcggtgcagt cgtcggtgta ccccaggcgt ctgcggattg
    55861 cgcggcactg tccgccgctg gcgtggtatc ccacggatta ttttgatcag ccattattta
    55921 ccccctcacg atctaaagcg cgcagcagca ttcctttcga aatgatgccg acatactgtt
    55981 ggtcctcgtc gaccacgggc accgcacagg gtgcctgtcc gacatgagag agcaactcgc
    56041 taagaggcgt ttgtgcatcg actgctaacg gcgcatcaat cagcgccgca tcaagacctt
    56101 gctgctgcgt taacgcggtt ttaagcgaat cgatggagac tgcgccgaca aacttattac
    56161 cgcgttcgat aacgtagcca tattcgcgat cttcatcctg caataatttc agtgccgaac
    56221 gtgggccgaa gccaggggtt ttacgaatta agccattcgg tgtccggcgg gcaatatctt
    56281 tcgcactgaa tacctgacta atatcaacgc cacggaagaa ggtacggaca taatcattcg
    56341 ccggattatt gagaatttca tccggtgtgc cgacctgtac cacttcacca ttttgcataa
    56401 tggcaattcg gtcgccaata cgcatggctt catcgagatc gtgggaaata aagacaatgg
    56461 tgcgctgatg tttcgcctgt aattttacca gctcatcctg catctcggtg cgaattaatg
    56521 gatcgagcgc cgagaaggct tcgtccatta ataaaatatc cggattaatc gctaacgcgc
    56581 gggctaatcc cacacgttga cgcatcccgc cagagagttc atccgggtag ctgtgggcat
    56641 aattttccag cccgacctga cgcagtgcat caagggcttt ttcccggcgt tcttcggcat
    56701 taattccggc caattccata ccgaacgcag tattgtccag cacggtcata tgcggcatta
    56761 aggcaaagga ctggaagacc atcgcaatct tttttctgcg cacctcacgg agtttggcat
    56821 cagatatttt ggcaatatcc acaccatcaa tcagcacttg cccgcgggtg ggttcaatca
    56881 ggcgattgag aaggcgtacc attgtggatt tacccgagcc ggataatccc atgatgacaa
    56941 atatctcgcc ttcttcaatg gccagactgg cgtcttttac gccaagcgat agcccagttt
    57001 tttccagaat ttgttctttt gaaagtcctt gttcgatata tttgaatgct cgctgtggat
    57061 gctcaccaaa tattttataa aggtttttaa tttctaattt aattgccatg caatagaaag
    57121 attcctgtta tttgtttatg tcgatatatt acccaatgga aatagttact tttttctacc
    57181 ctaacatact gagaatctga ggcaacccct gatggcaaat gtggcatgaa tattgatgcg
    57241 agcaaatcgc cggaatttcc cgtgatataa gggctgagag caaatcgaga aaagttgata
    57301 ttttttgtca aagatagcga aaaaacgcct gaatattgtg gtttcaggcg aataatgcaa
    57361 cagagtatca gtgtagatca ccacaaaata tttgcgtgat aaaaagctat ttggcgggaa
    57421 ttatttccct gtcgcgggta gtgatatttt tgaaaataac accctcagaa attccagtct
    57481 tcatcttctg tttcaaccgc tttccccatc acataagagg agcctgaacc ggaaaaaaaa
    57541 tcgtgatttt catcggcatt cggcgaaagc gcggcgagga ttgccggatt cacttccgcc
    57601 atttctgcgg gaaataaagg ttcgtagccc agattcatca aagccttatt ggcgttgtaa
    57661 cagagaaacg ctttcacatc gtcagcccac ggggtttcgg cgtacagctc atcggtgtat
    57721 tgcagctcgt tgtcgtagag ttccagcaac aaatcgaagg cgaaactctt caactcttca
    57781 cgttgtccca gagatatctt ttccatgttt ttctgatatt tatagcctat gtagtaaccg
    57841 tggactgctt catcacggat aatcagacgg atcaggtctg cggtattggt cagctttccg
    57901 cggctggaaa aatacatcgg cagccagaaa ccggaataga acaaaaaaga ttcaagaaac
    57961 acactggcga ttttcttttt cagcggatca tcaccgcgat aatgttgctg aataatctga
    58021 gcttttcgct gcaacggtgc gttttcttca ctccaggcgt aggcggcatc gacatctttg
    58081 gtctggcaca gcgtcgagaa aatcgaactg taagagcggg catgaaccgc ttccataaag
    58141 ctgatattcg ataataccgc ttcttcatga ggcgtgagtg catcgggcat cagcgaaggc
    58201 gcgccgataa cattttgcag cgtgtcgagc agcgtcaggc cagtaaaaac gcgcatcgtc
    58261 agttgttgtt ctacgacagt taatgtctgc caggcaggaa tatcgttcga cagcggcacc
    58321 ttttctggta gccagaaatt gctggtcagg cgattccaca cctccagatc tttatcgtca
    58381 gatatcttgt tccagttgat ggcgctgata cgtgagagtt tcatagatat tccttaaagt
    58441 gcacaggaga cgcagccttc aatttcagtg ccttccagcg ccatctgacg caggcgaatg
    58501 taatagagcg ttttgatacc tttgcgccag gcgtaaatct gcgctttgtt gatatcgcga
    58561 gtggtggcgg tatcggggaa aaacagcgtc agcgacaaac cctgatcgac atggcgagtc
    58621 gcttccgcgt aggtgtcgat gatcttttct gcgccgattt cgtaagcatc ctgatacagc
    58681 gccagatttt cgttagtcat aaacggggca gggtagtaaa cgcgtcctgt tttgccctct
    58741 ttgcgtatct ctactttcgc cacaatcgga tgaatactcg acgtagcatg gttgatataa
    58801 gagatagaac cggttggcgg caccgcctga agattctggt tgtaaatgcc gtagcgcatc
    58861 acgtcgtcgc gcagttgcgc ccacatctca cgggtaggta acgtaatacc gctgcgggca
    58921 aacagttcgc caactttcgc cgttttcggc tgccagttac cttgcagata ttggctaaaa
    58981 tattcaccac tggcataacg tgactgtttg aacccggcga aggtttcacc gcgttcgcgc
    59041 gccagcaaca tcgaggtacg cagtgcgtgc caggtgatgg catagaaata gagattggtg
    59101 aaatccagtg cttccggcga accataagcg atgccttctc gcgccagata gccgtgtaaa
    59161 ttcatctgcc ccagtccgat ggcgtgtgag gcggcatttc cggcttcgat ggacggcacg
    59221 ctgcggatat gactcatatc tgacaccgcc gttaaaccgc gcacggcagt ctctaccgtg
    59281 cgggcaaaat cgggggaatc catggtgtgc gcaatattca acgaacctaa attgcaggag
    59341 atatcatggc ctgtgcgggc atagtcgaga ttctcgtcat actctgaggc acagttgacc
    59401 tgcaaaatct ctgagcagag attactcata tttatgcgcc cggcgacagg gttagcacgg
    59461 tttaccgtgt cttcatacat gatgtaggga tagccggact caaactggat ttctgccagc
    59521 cgctggaaga aatcacgggc gttgaggtat tttttgcgga tgcgttcatc ggcaaccagt
    59581 tcgtcatagt gttcgctgat ggcgatatcg gcaaacggct tgccataaac tcgctcaacg
    59641 tcatagggcg aaaacagcgc catctgcgca ttctcttttg ccagatggaa agtgatatcc
    59701 gggatcacca cgcccagcga cagtgtttta atgcggattt tttcgtcggc attttcccgt
    59761 tttgtatcga gaaaacgcag aatatcggga tgatgagcat gtaaatagac tgcacctgcc
    59821 ccctgacgcg cgccgagttg gttggcatag gaaaatgcgt cttccagcat tttcatcacc
    59881 ggaattacgc cagaagattg attttcaata cgtttaattg gcgcgcccgc ttctcgcaga
    59941 ttcgacagca aaaatgccac gccgccgccg cgtttcgaca gttgcagtgc ggaatttacc
    60001 gcccggccaa tcgactccat attgtcttca atacgcagca gaaaacagga aaccagttcg
    60061 ccgcgctgct gcttaccgca gttgaggaat gtcggcgtgg ctggctgaaa gcgtcctgac
    60121 agcatttcat tggtcagttg caacgccagc gtcgcatcgc cttgtgccag cgtcagcgcc
    60181 accatcgtga ctcgatcggc aaaatcttcc agataacgtt taccgtcgaa tgtcttcaac
    60241 gtatagctgg tgtaaaactt ccatgccccg aggaatgtct ggaaacgaaa accgctggtg
    60301 tgtgcgtggg caaacagcgt aatgacaaag tcgcgagagt agcgattaag aacgctttca
    60361 tcatagtaac cttcgttgac cagccagttc aggcgctgct gctggctact gaaggtcaca
    60421 ctgttcggac gcacatgcgt cgccataaag gcgtcaacgg cctggcggtc tttatcgaac
    60481 tgaatgcgac ctgcgctatc gtagaggtta agcatcgcat tcagcgcgtg gtaatccatc
    60541 gtttcctgcg tcaggcattc tgcggttgtc gttgccaaaa ttcggttact cctttacgaa
    60601 cgttttcgat atcgctttgc gtacccatga gttcaaaacg gtacagccac ggcacgccgc
    60661 atttccgggc aatcacatct ccggcgcggc catacgcctc accaaagttg cgattaccag
    60721 aagcaataac gccgcgaagc aacgcccggt tgtgctcgtc gtttaaaaag cgaattacct
    60781 atcgtggcac cgcgccagcc gtaccgccgc cgccgtaaga gggcacgatc aggatgtaag
    60841 gctcgtctac ctgaatccgt tcccgctcat tgagcgggat gcgcaccgcg ggcagaccta
    60901 aacgttcgat aaaacgctgc gtgttttcgg agctgctgga gaagtagacg agctggctca
    60961 tgcactggcc gcgtgtggcg ctggatgcag acggttaatc atgtccggac ggaaaccaga
    61021 ccagctaaga tcgccagcaa tcactaccgg caactgacga aagccctgag cacgcaacgc
    61081 ttccgctgct tcaggaacgc gatcgacatt aatcatttca aaatcaaagc cccggttttc
    61141 catcgcccgt ttggtggcgt ggcactgaac gcaatcgtta cgagtgtaaa tagttatgcg
    61201 catgattcgt atttccgttt aaaatgaaga tacggcgcga tgatacgcgt cgggttgtct
    61261 ctctgttgat acagagatac tagatgtagt tgaaaaaaga ttcaaccaca caatatatag
    61321 caaatagtga tcgaaattac cctagatatg agcgtgacgg gggaggggga tttttgtgat
    61381 tcaccaggca aaaagaaacc ccgaagacag gcttcggggt taaagacgcg tatttattat
    61441 catttttgca ctacgatttg cgcatgctta acagtgcgcc gataaaaata cctaccgcag
    61501 ctgctgtacc cacgctacac cagggtcttt cacgaacaaa agaatccgcg cagccaacgg
    61561 catcgcgtgc ggcttgctgg acgcgagtac gaccatgcat tcgcgccctg gtttctttca
    61621 gcaatgcctg agctttactg cgtgcggctt cagcttcccc tttggcgtcg ctgccccagg
    61681 acttcaatac agattcgagg ctgtcagcta attgattgac atcattctgg atatcctgca
    61741 cgccatcatc aacgtcgttg cggttcggtc ggttaaacat atgatccccc attagaattc
    61801 gatgtaattt tcagtgtaga ccataatttt cttaactgag gttcatcgcg gctttatgga
    61861 cttttctgaa agcgttgtga atgcgcaatc aggtaaggta ggcccgcggt tgataataac
    61921 gaggatcaaa tatgtattta cgaccagacg aggtggcgcg cgtacttgaa aaagtcggtt
    61981 ttactgtcga tgtggtaacc cagaaagctt atggttaccg ccgtggggaa aattatgtct
    62041 atgttaatcg cgaagcgcga atgggacgta ccgcactggt aattcatcca acattaaaag
    62101 aacgtagttc gacactggct gaacccgctt ccgatattaa aacttgcgat cattatcaac
    62161 aatttccgct ctatttagca ggcgagcgac acgagcatta cggtatcccg catggcttca
    62221 gttcgcgtgt tgcgcttgaa cgttatttga atggtttatt tggcgaagcc agttaaagac
    62281 gcgactggcg atgccagtcg cgaaaagaag agtcaggctt ttacctgctg gtaatggctg
    62341 actttaaaca gtcggcggca ataatcgagg aagtagccat aaaccgcccc catcaacatc
    62401 gaaacaacga tgtttgaact gaccgccgcc ataatctgat gccagtctgc gcccaccact
    62461 aacaagatcg ccacatacac cggtgactgg aacgtcacat aagccaggat atcagccaga
    62521 ttttttatcc agcccgacgg gctaactttg cgtgccgcgc gcataaatag atcgcggtac
    62581 ataccgtatg gccatgcaat taagatgttc accggaatcg ctaccaatct ggaataaaaa
    62641 gactgttcga agctcattcc ggagaggaaa acttcaatac acatgttcac gacagaacag
    62701 taaacaacca tcgcgaacgt atctgcaact gcatgacgca agcgtgactg cggtgagaac
    62761 atgctaatgc tccttaataa aagcgtaatg ggatgttaat ggagatgaaa gcagtggatc
    62821 tcattgccat ctgcattttc gcatagcgta tatcgctggc tatgactatt caattagtga
    62881 aaaattttta tttagtcagt ttttgtagat aaaatattcc gtaacggctc gttttttgta
    62941 cattgtggat ttttacatgc tgatatgatt tattttcaat taaatcaatt agatatgacc
    63001 ttgcctttaa tttcactaat gaaagtttgg gggtgaggta acgctataag cgtagagaat
    63061 tcggtgtatg tcgtgaccgt gtcatttaca gcatttttga tgccagaaaa tgtacattaa
    63121 cagcagaaag caccagtgag ggggg
//
LOCUS       DAETQN010000008        60958 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.008, whole genome shotgun sequence.
ACCESSION   DAETQN010000008 DAETQN010000000
VERSION     DAETQN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.008"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..468
                     /locus_tag="K0W68_000553"
     CDS             <1..468
                     /locus_tag="K0W68_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3072213.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="K0W68_000554"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="K0W68_000554"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBI3072214.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="K0W68_000555"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="K0W68_000555"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBI3072215.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="K0W68_000556"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="K0W68_000556"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBI3072216.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="K0W68_000557"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="K0W68_000557"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBI3072217.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="K0W68_000558"
     CDS             complement(4084..5313)
                     /locus_tag="K0W68_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBI3072218.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="K0W68_000559"
     CDS             complement(5377..5817)
                     /locus_tag="K0W68_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBI3072219.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="K0W68_000560"
     CDS             complement(5922..6692)
                     /locus_tag="K0W68_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3072220.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="K0W68_000561"
     CDS             complement(6689..7615)
                     /locus_tag="K0W68_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3072221.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="K0W68_000562"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="K0W68_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBI3072222.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="K0W68_000563"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="K0W68_000563"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBI3072223.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="K0W68_000564"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="K0W68_000564"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBI3072224.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="K0W68_000565"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="K0W68_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBI3072225.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="K0W68_000566"
     CDS             13322..13669
                     /locus_tag="K0W68_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBI3072226.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="K0W68_000567"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="K0W68_000567"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBI3072227.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="K0W68_000568"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="K0W68_000568"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBI3072228.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /locus_tag="K0W68_000569"
     CDS             complement(15489..15851)
                     /locus_tag="K0W68_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBI3072229.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="K0W68_000570"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="K0W68_000570"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBI3072230.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKIEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="K0W68_000571"
     CDS             18978..20735
                     /locus_tag="K0W68_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HBI3072231.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="K0W68_000572"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="K0W68_000572"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBI3072232.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="K0W68_000573"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="K0W68_000573"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBI3072233.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="K0W68_000574"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="K0W68_000574"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBI3072234.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="K0W68_000575"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="K0W68_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBI3072235.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28591..29964
                     /gene="aroP"
                     /locus_tag="K0W68_000576"
     CDS             28591..29964
                     /gene="aroP"
                     /locus_tag="K0W68_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBI3072236.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="K0W68_000577"
     CDS             complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="K0W68_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBI3072237.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="K0W68_000578"
     CDS             complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="K0W68_000578"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBI3072238.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31497..32390
                     /gene="nadC"
                     /locus_tag="K0W68_000579"
     CDS             31497..32390
                     /gene="nadC"
                     /locus_tag="K0W68_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBI3072239.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32593..33033
                     /gene="ppdD"
                     /locus_tag="K0W68_000580"
     CDS             32593..33033
                     /gene="ppdD"
                     /locus_tag="K0W68_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBI3072240.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33043..34429
                     /gene="gspE"
                     /locus_tag="K0W68_000581"
                     /pseudo
     CDS             33043..34429
                     /gene="gspE"
                     /locus_tag="K0W68_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            34419..35621
                     /gene="hofC"
                     /locus_tag="K0W68_000582"
                     /pseudo
     CDS             34419..35621
                     /gene="hofC"
                     /locus_tag="K0W68_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(35656..36699)
                     /gene="guaC"
                     /locus_tag="K0W68_000583"
     CDS             complement(35656..36699)
                     /gene="guaC"
                     /locus_tag="K0W68_000583"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBI3072241.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36855..36899)
                     /locus_tag="K0W68_000584"
     CDS             complement(36855..36899)
                     /locus_tag="K0W68_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072242.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36924..37544
                     /gene="coaE"
                     /locus_tag="K0W68_000585"
     CDS             36924..37544
                     /gene="coaE"
                     /locus_tag="K0W68_000585"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBI3072243.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37544..38287
                     /gene="zapD"
                     /locus_tag="K0W68_000586"
     CDS             37544..38287
                     /gene="zapD"
                     /locus_tag="K0W68_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBI3072244.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38297..38494
                     /gene="yacG"
                     /locus_tag="K0W68_000587"
     CDS             38297..38494
                     /gene="yacG"
                     /locus_tag="K0W68_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBI3072245.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38594..38983)
                     /gene="mutT"
                     /locus_tag="K0W68_000588"
     CDS             complement(38594..38983)
                     /gene="mutT"
                     /locus_tag="K0W68_000588"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBI3072246.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39043..41748)
                     /gene="secA"
                     /locus_tag="K0W68_000589"
     CDS             complement(39043..41748)
                     /gene="secA"
                     /locus_tag="K0W68_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBI3072247.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41810..42322)
                     /gene="secM"
                     /locus_tag="K0W68_000590"
     CDS             complement(41810..42322)
                     /gene="secM"
                     /locus_tag="K0W68_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBI3072248.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42553..43470)
                     /gene="lpxC"
                     /locus_tag="K0W68_000591"
     CDS             complement(42553..43470)
                     /gene="lpxC"
                     /locus_tag="K0W68_000591"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBI3072249.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43571..44722)
                     /gene="ftsZ"
                     /locus_tag="K0W68_000592"
     CDS             complement(43571..44722)
                     /gene="ftsZ"
                     /locus_tag="K0W68_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBI3072250.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44783..46045)
                     /gene="ftsA"
                     /locus_tag="K0W68_000593"
     CDS             complement(44783..46045)
                     /gene="ftsA"
                     /locus_tag="K0W68_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBI3072251.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46042..46872)
                     /gene="ftsQ"
                     /locus_tag="K0W68_000594"
     CDS             complement(46042..46872)
                     /gene="ftsQ"
                     /locus_tag="K0W68_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBI3072252.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46874..47794)
                     /gene="ddlB"
                     /locus_tag="K0W68_000595"
     CDS             complement(46874..47794)
                     /gene="ddlB"
                     /locus_tag="K0W68_000595"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBI3072253.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47787..49262)
                     /gene="murC"
                     /locus_tag="K0W68_000596"
     CDS             complement(47787..49262)
                     /gene="murC"
                     /locus_tag="K0W68_000596"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBI3072254.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49316..50383)
                     /gene="murG"
                     /locus_tag="K0W68_000597"
     CDS             complement(49316..50383)
                     /gene="murG"
                     /locus_tag="K0W68_000597"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBI3072255.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50380..51624)
                     /gene="ftsW"
                     /locus_tag="K0W68_000598"
     CDS             complement(50380..51624)
                     /gene="ftsW"
                     /locus_tag="K0W68_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBI3072256.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51624..52940)
                     /gene="murD"
                     /locus_tag="K0W68_000599"
     CDS             complement(51624..52940)
                     /gene="murD"
                     /locus_tag="K0W68_000599"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBI3072257.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52943..54025)
                     /gene="mraY"
                     /locus_tag="K0W68_000600"
     CDS             complement(52943..54025)
                     /gene="mraY"
                     /locus_tag="K0W68_000600"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBI3072258.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54019..55377)
                     /gene="murF"
                     /locus_tag="K0W68_000601"
     CDS             complement(54019..55377)
                     /gene="murF"
                     /locus_tag="K0W68_000601"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBI3072259.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55374..56867)
                     /gene="murE"
                     /locus_tag="K0W68_000602"
     CDS             complement(55374..56867)
                     /gene="murE"
                     /locus_tag="K0W68_000602"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBI3072260.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56854..58620)
                     /gene="ftsI"
                     /locus_tag="K0W68_000603"
     CDS             complement(56854..58620)
                     /gene="ftsI"
                     /locus_tag="K0W68_000603"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBI3072261.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58636..59001)
                     /gene="ftsL"
                     /locus_tag="K0W68_000604"
     CDS             complement(58636..59001)
                     /gene="ftsL"
                     /locus_tag="K0W68_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBI3072262.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58998..59939)
                     /gene="rsmH"
                     /locus_tag="K0W68_000605"
     CDS             complement(58998..59939)
                     /gene="rsmH"
                     /locus_tag="K0W68_000605"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBI3072263.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59941..60399)
                     /gene="mraZ"
                     /locus_tag="K0W68_000606"
     CDS             complement(59941..60399)
                     /gene="mraZ"
                     /locus_tag="K0W68_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBI3072264.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14596 a        16854 c        14997 g        14511 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcgagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttcctgc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtactt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaatt
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa
    28261 cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt tttgattttt
    28321 aagtcaataa aaacgaattt aaattcattc tgcatattga gaggggttga ggctgagctt
    28381 tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc
    28441 tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcttt
    28501 gatgtaaaaa aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag
    28561 atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag
    28621 ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg
    28681 acagggctat tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg
    28741 ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg
    28801 gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt
    28861 gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag
    28921 ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct
    28981 gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc
    29041 gagatggagt tctggtttgc cattatcaaa gttattgcga tagtagcgat gatcatcttc
    29101 ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg
    29161 gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc
    29221 atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg
    29281 gagcaaagta tcccgaaagc gactaaccag gttatctacc gcatcctgat tttctatatt
    29341 ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt
    29401 ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc
    29461 gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg
    29521 ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgc
    29581 ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt
    29641 aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg
    29701 gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa
    29761 caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg
    29821 ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta
    29881 tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc
    29941 gccaaagccg taaaagcgca ttaatctctc tacgccctca cccatgcagg gtgagggcaa
    30001 taatctttac accaacgctc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa
    30061 cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg
    30121 ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa
    30181 gtcacccagc gaagcaaacc aggccggtaa cgctttctcc ccatgaccga tcaaggcata
    30241 taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc
    30301 ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa
    30361 aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca accagaacag
    30421 cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata
    30481 ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt
    30541 ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg
    30601 aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac
    30661 gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat
    30721 ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag
    30781 acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag
    30841 taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa
    30901 tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg
    30961 ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga
    31021 tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa
    31081 tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaggc acgtttatcg
    31141 aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc
    31201 aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat
    31261 gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc
    31321 agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg
    31381 ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc
    31441 cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc
    31501 cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg
    31561 atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca
    31621 acaatgacat tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acggtgagca
    31681 cccgcgagaa tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg
    31741 caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc
    31801 aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc
    31861 ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac
    31921 tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag
    31981 ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg
    32041 ccttcctgat caaagaaaat catattattg cctccggctc agtgcgccag gccgtcgaaa
    32101 aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac
    32161 ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac
    32221 agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg
    32281 tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg
    32341 cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag
    32401 atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct
    32461 cgcaaatttc ttgtcttttg ctgcaacgaa ataaaaaacc tcggaagctg ctttcccgtt
    32521 tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca accaaatcaa
    32581 ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt
    32641 ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa
    32701 gctgcactca ccgacatgct acaaaccttt gtgccttacc gtacagccgt agagttgtgc
    32761 gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct
    32821 accaccaccc gctatgtttc agccatgagt gtggcaaaag gcgtggtgtc gctgaccggg
    32881 caagaaagtc tcaatggact aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc
    32941 gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc
    33001 gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc
    33061 accgccctgt gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga ggtggttcat
    33121 gttgcggtag tcgatgcacc ttcgcatgag ttgctggacg cattgcattt cgctaccacc
    33181 aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggccacgc cagtcgcaca
    33241 caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctgact
    33301 cgaacgttac aatctgcgct ggaacaacgt gcgtctgata ttcatatcga accagcggac
    33361 aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca
    33421 ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg
    33481 gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca
    33541 tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag
    33601 caggtgaacc aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac
    33661 tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc
    33721 ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga cattaatatt
    33781 tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac gcaaatccat
    33841 ccgcgtgccg ggctcacctt tcagggcgtt ttgcgtgcgt tattgcgcca ggatcctgac
    33901 gtcatcatga tcggagagat ccgctatggc tttaacggca gaaattgcca ttaaagccgc
    33961 gcaaaccggt cacctggtgt tgtctaccct acacactaat tccacctgcg aaacgctggt
    34021 acgtttacag caaatgggag tcgcccgctg gatgctctca tcagcgctta cgctggtaat
    34081 agcccagcgt ctggtacgta aactttgccc acattgtcgc cagcagcaag gggagcccat
    34141 ccatattcca gtcaatgtat ggccgtcgcc gctgccccac tggcaggcac ccggttgtgt
    34201 acattgctac cacggttttt atggtcgtac ggccttattt gaagttctgc ccataacgcc
    34261 ggtcattcgt cagcttattt ccgctaatac cgacgttgaa tcgctggaaa cgcacgcacg
    34321 acaggcgggt atgcgtacgc tttttgaaaa cggctgcctg gccgtggagc aaggcttaac
    34381 cacctttgaa gagttaatcc gcgtactggg gatgccgcat ggcgagtaag caactctggc
    34441 gctggcatgg cataaccggc gacggcaatg cgcaagatgg gatgctatga gcagagagcc
    34501 gtgctttgct gctcatggca ctacagcaac agatggttac cccacttagc ctgaagcgaa
    34561 tcgccatcaa ttctgcgcag tggcgaggag ataaaagcgc ggaagtcatt catcaactgg
    34621 cgacgctact caaagccggg ttaacgcttt ctgaagggct ggcactgctg gcggaacagc
    34681 atcccagtaa gcaatggcaa gcgttgctgc aatcgctggc gcacgatctc gaacagggca
    34741 ttgctttttc caatgcctta ttaccctggt cagaggtatt tccgccactc tatcaggcga
    34801 tgatccgcac gggtgaactg accggtaagc tggatgaatg ctgctttgaa ctggcgcgtc
    34861 agcaaaaagc ccagcgtcag ttgaccgaca aagtgaaatc agcgttacgt tatcccatca
    34921 tcattttagc gatggcaatc atggtggttg tggcaatgct gcattttgtt ctgccggagt
    34981 ttgccgctat ctataagacc ttcaacaccc cactaccggc actaacgcag gggatcatga
    35041 cgctggcaga ctttagtggc gaatggagct ggctgctggt gttgttcggc tttctgctgg
    35101 cgatagccaa taagttgctg atgcgccgac cgacctggct tatagtgcgg cagaaattgc
    35161 tgttacgcat cccgattatg ggttcactga tgcggggaca aaaactcacg cagatcttta
    35221 cgattctggc gctgacacaa agtgcaggca ttactttttt gcagggcgta gagagcgtca
    35281 gagaaacaat gcgctgcccg tactgggtgc aacttctgac acaaatccag cacgatatca
    35341 gtaacggtca tcccatctgg ctggcgctaa aaaataccgg ggagtttagc ccgctctgtt
    35401 tgcaattagt gagaacagga gaggcatccg gctcgctgga cctcatgtta gacaacctcg
    35461 cccatcatca tcgggataac acaatggcgc tggcggataa cctcgcagcc ttactggaac
    35521 cgacgttgct gatcataacg ggaggaatta tcggtacgct ggtggtggcg atgtatctgc
    35581 caattttcca tttaggcgat gcgatgagtg ggatgggata acgctggcgt gttgctccac
    35641 accagcgttg gaagattaca ggttgttgaa gatgcggttt tcttgttcct gcacacggat
    35701 aaatgtggtg cgcttggtca gttctttcag gcgtgaagcc ccaacgtatg tacaagctga
    35761 acgcaggccg cccaaaatat cacgcgcggt attttcaacc gggcctcgca gcggcagctt
    35821 aacggtttta ccttctgctg cgcgatattc cgcaacgccg ccaacgtgac gtttcatcgc
    35881 agactcggag ctcatgccgt agaacagcat aaatttctcg ccgttctcct caacgatgcg
    35941 accgccgctc tcttcgtggc ccgccagcat gccgccaagc atgacgaaat cggcaccgcc
    36001 gccaaaggct tttgcgacat cgcccggcgt ggtgcagcca ccgtcgctga cgatcattcc
    36061 gcccagaccg tgcgccgcat cggcacattc gattaccgca gaaagctgcg gataaccgac
    36121 gcctgttttg acgcgagttg tacaaacaga acctgggcca atgccaactt taacgatatc
    36181 agcaccggag aggataagct cctcacacat ttcaccagtc actacgttgc cagcacaaat
    36241 ggttttggtc ggccacgctt cacgcgcttt cgcaacgaac tgcacgaagt gttcggaata
    36301 accattcgcc acatcaatac aaacgaagtt taatgccggg ttcaggtcga gaatctgttt
    36361 agttttttcg aaatccgcat cagacgtacc ggtagaaacc atcacatgtt tcagcacatc
    36421 agcggaagaa ttgttgataa acgcttgcca ctcttcgaca gaatagtgtt tatgcacagc
    36481 agtcaaaata tcaaaagaag ccagcgcaga ggccatagaa aatgtaccta cggtgtccat
    36541 atttgcggcg ataatcggca cgccggacca gctctgacct gaatgtttga aggtgaattg
    36601 acgttccagt tcaacatcgg aacggctttt aagagtggag cgtttagggc ggatgagaac
    36661 gtctttaaaa cctaacttca gatcttcttc aatacgcatg tgcggattcc tggggttaat
    36721 ggctataagg gtaagagcgc aactccagtg acgctatcat acgcactaat caatgcggcg
    36781 caagactgcg aaattgtctt tttttacgct acaatcccat aaatttacct ggtaaatagt
    36841 gagcgaacgt ttcatcaatt tcgctttcgt gcactcattt ttaacgtttt gataatcatg
    36901 atgaattccc gggaatataa attatgaggt atatagttgc cttaacggga ggcattggca
    36961 gtggcaagag taccgttgcc aatgcgtttg ctgatctcgg aattaacgtc attgatgccg
    37021 atattattgc gcgtcaggtg gttgaaccag gtgcacctgc gctacatgcc attgctgatc
    37081 actttggcgc taacatgatt gctgctgatg gaacattgca gcgccgggcc ttgcgcgagc
    37141 ggatcttcgc caacccggaa gagaaaaact ggcttaacgc cctgctgcat ccgctgattc
    37201 agcaagagac gcaacaccag atccagcaag ctacttcccc ctatgtactg tgggttgtgc
    37261 cattgctggt agaaaactca ctgtataaaa aagcgaatcg agtgctggtg gtggatgtca
    37321 gcccagaaac gcaacttaag cgcaccatgc agcgcgatga tgtaactcgc gagcatgtcg
    37381 aacaaatcct tgctgctcag gcaacgcgcg aagcccgcct tgccgtggca gatgacgtca
    37441 ttgataataa cggcgcaccg gatgctatcg catcggatgt tgcccgcctg cacgcacact
    37501 atttgcagct tgcgtcgcag tttgtctcac aggaaaaacc gtaatgcaga cccaggtcct
    37561 ttttgaacat ccactaaatg aaaaaatgcg tacatggctg cgcattgagt ttttgattca
    37621 gcaactcacc gttaatttac ccatcgttga ccacgctggc gcgctgcatt tcttccgtaa
    37681 tgtcagtgaa ttactggatg ttttcgagcg cggcgaagtc cgcactgagc tgttgaaaga
    37741 acttgaccgg cagcaacgta aactccagac ctggattggc gtgcctggcg tggaccagag
    37801 ccgtattgaa gcattaattc agcagttaaa agcggcgggg agcgtattaa tttccgcgcc
    37861 gcgtatcggg caatttctgc gtgaagatcg tttgattgct ctggtgcgtc agcgactgag
    37921 catcccaggc ggctgttgca gctttgattt acctacattg cacatttggc tgcatctacc
    37981 ccaggcgcag cgcgacagcc aggtagaaac ctggattgcc agcctgaacc cgctcaccca
    38041 ggcacttacc atggtgctgg atttaattcg ccagtcggcc cccttccgta aacaaaccag
    38101 tctgaatggt ttttatcagg ataacggtgg cgatgccgac ttgctgcgcc tgaatctgtc
    38161 gctcgattca cagctttatc cgcaaatttc cggacataag agccgttttg ccattcgttt
    38221 tatgccgctg gacactgaaa acggacaggt accggaacgt ctggatttcg aactggcctg
    38281 ttgctaagga gtaaaaatgt cagaaactat tacggtgaat tgcccaacct gcgggaaaac
    38341 ggtggtgtgg ggtgaaatca gcccgtttcg gccattttgc tccaaacgtt gtcagctgat
    38401 cgacctcgga gaatgggctg ctgaagaaaa acgaatcccc agcagtggcg atctttccga
    38461 aagcgatgac tggagcgaag agccaaagca gtgacatttg tctgatgccg cacgtaggcc
    38521 tgataagacg cggacagcgt cgcatcaggc atcttgtgca aatgtcggat gcggcgtgaa
    38581 cgccttatcc gacctacaga cgcttaagct tcgcaattac cggttcattg gctggcggaa
    38641 aatcatcggc attaagaccg actagcgaca tccactcacc gggttgccct tctttacccc
    38701 acggctcccc ttcccagctt tcgaccagcc agaaccacag agttatatgc ctgtccggga
    38761 attcatattc cagtttttca aatagcgaaa aatgttgtgg ggtaatcccg acttcttcct
    38821 gaagttcacg cactaccgcc tgttccggcg tttcgcccat ttcaatttta ccgccgggaa
    38881 actccagttt attcgccatg tgcgcatctg ctgcgcgacg cgttataaag atttcattgt
    38941 tctcgttgcg aataatacct accgcaattt gcagcttttt cattgtctta aacctataaa
    39001 aaaggcgcag aatcctgcgc cttttacttc aactgttagc ttttattgca ggcgaccatg
    39061 gcactgcttg tattttttac cagaaccgca cgggcaagga tcgttacgtc ctactttgcg
    39121 ctcaccggtt tgcgccgcca gtgcagctgc ggctgcagag tcgtcatcct gatggctaag
    39181 ctgctgcatt tgcgctaaac gctcggcttc catacgacgc tgttgttcca gctcctcaac
    39241 ctcttcaggc atacgtacct ggactttgct cagcgtgctg ataacttcat attttaacga
    39301 ctccagcatc gctgcaaaca tggagaacga ttcacgtttg tattcctgct tcggatcttt
    39361 ctgtgcatag ccacgcaggt ggataccctg acgcagatag tccatcgccg ctaggtgctc
    39421 tttccacaga gagtcgagag tttgcagcat gacgcctttt tcgaagtgac gcatcatctc
    39481 agcaccaacc acttcttctt tacgctgata cacttcgatg gactgcgcca gaattcgctc
    39541 acgcagcgtc tcttcatgca gttctggttc tttatccagc cactcggcaa ttggcaaatc
    39601 gaggtcgaaa tcgttcttca gacgttcctg cagccccgga atatcccaca tttcttccag
    39661 tgactgtggc ggaatgtagg catcaatggt cgctttgaac acatcttcac gaatgctgtt
    39721 gatggtttcg ctcacatcgc tgacatccag cagttcgtta cgctgggagt aaatggcgcg
    39781 acgctgatcg ttagccacgt catcatattc cagcagttgc ttacgaatgt cgaagttacg
    39841 gctttcaact ttacgctggg cgttggcaat cgctttagtc acccacgggt gttcaatggc
    39901 ttcgcctggc ttcataccca gtttacgcat catgccggat actcggtcgg aagcaaaaat
    39961 acgcatcagc gcatcttcca tcgacaggta gaaacgggaa gaaccagcat ccccctgacg
    40021 accagaacga ccgcgcaact ggttatcgat acgacgggat tcgtgacgct cggtaccgat
    40081 gatatgcagg ccacctgctt ccagtaccgc atcgtgacgt acctgccagt cggctttaat
    40141 tttttcaatt tgctctgcgg tcggattttc cagcgcggca acttctgcct gccagctacc
    40201 accgagcaca atatctgtac cacgacccgc catattggtc gcgatagtca ccgcagccgg
    40261 ataacctgcc tgagcaacaa tcgccgcttc gttggcgtgg aatttggcgt tcaggacgtt
    40321 gtgcttaata ccggctttgg tcagttcgtt tgacaccagc tccgattttt cgatggagat
    40381 agtacccacc agcaccggct ggcctttcgc agtacgttct ttgatatctt caatgatcgc
    40441 ctgaattttt tccgcttcag tcatgtagac caggtccggc agatctttac gaatcattgg
    40501 acggttggtc ggaacaacga cggtatccag cttgtagatg gagctaaatt cgaaagcttc
    40561 ggtatcagca gtaccggtca tccccgccag tttttcatac agacggaagt agttctggaa
    40621 ggtgatcgaa gccagcgttt ggttttcgtt ctggatctgc acaccttctt tcgcttccac
    40681 agcctggtgc agaccatcgg accagcgacg gccctgcatg gtacgaccgg tgtgttcgtc
    40741 aacgatgata acttcaccat ctttaacgat gtagtcgacg tcgcgggtaa acagcgcatg
    40801 agcgcgcagc gccgccgtta cgtggtgcat cagcatgatg ttggccggag agtacagaga
    40861 ctccccttca tccatgatgc cctctttcac cagcagttct tcaatcagca ccagaccacg
    40921 ttcggtcagg ttcacctggc gagatttttc gtccaccgag aagtggcctt cgccctggaa
    40981 ggtttcggag tcttcttttt cctgacggat caggtgcgga ataattttat tcacgcgttt
    41041 atacatttcc gagctgtctt ctgccgggcc ggaaatgatc agcggtgtac gcgcttcatc
    41101 gatcaggatg gagtccactt cgtccaccag cgcatagtgc agtttacgct gtacacgttc
    41161 ttcagggctg aacgccatat tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc
    41221 gtaagtgatg tcagctgcgt aagcttcgcg ctttgccggt gctggcatgc ccggcaggtt
    41281 gataccgaca gtcaggccaa ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg
    41341 cgccaggtag tcgttgacgg taactacgtg cacgccttta ccggttagtg cgttcaggta
    41401 agcaggcagc gttgcggtca gggttttacc ttcaccggta cgcatttcgg cgatgcagcg
    41461 ttcgttaaga accataccgc cgagtaactg aacgtcgaag tgacgcatac caaagacgcg
    41521 cttacttgcc tcacgtacca cggcgaaagc ttccgggatc agattttcca gcacttcgcc
    41581 tttttccaga cgtgcacgaa actctgcggt tttccctttc agttcttcgt cggagagttt
    41641 ttccatctcc ggttccatgg cattgatgat gttgaccact ttgcgcatcc ggcgcagggt
    41701 gcgatcgtta cgactaccga aaactttagt taacaatttg attagcataa taaaatctca
    41761 aacgccccgc gttgcggagt taataaaatg aagtaaaggt ttattgttgt taggtgaggc
    41821 gttgagggcc agcacggatg ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg
    41881 cttgtggggt aaaatgcgca taatcaatgc gataaccctt ttcagacggc gtgccttcct
    41941 gggtcagcag cgcgctgagc gtatccagta atgcaagatg ttgcgcctga agaggcaaag
    42001 actcttcagc aacgggcagt gtttgcggtg ccattgcgaa agaaagatga cggattaccg
    42061 tgcgaatggc atgttgatgc cagtaatcaa cggaatagtt cgaattcggg cggcgtgtgt
    42121 tcgcttccag caaggccaat tgaccaaagt taactttggc tgaaggctcg tggttgcggg
    42181 ttgtcgcttt tgcgggcgcg tttggttcgg cggcgttgct gagcgcaggc aaacctaaac
    42241 tcgccgcaac catccctaat aagagatgcg gccagaagta gcgtttacca aactgtcgcc
    42301 agcgcgtcag tattccactc acgttattgc catcccgatc gtattcaaac gtatttagcg
    42361 cacaaatctt atcattaaag cctgcagtcc acagcacgaa tgaagataag ttgtgcggaa
    42421 aagtgcttaa gaaaacagcg ttcgcaccgt tttttatagc tcttataaaa ttcaaaaaaa
    42481 aatgactggt tcacctggcc ggagagagtg ccagatttgc cagtcgaatt ttatacgaca
    42541 gtataaatgt cgttatgcca gtacagctga aggcgctttg aaggccaacg gcagttctgc
    42601 gtcgtcctgg aaggtcacat attcccaggc ttcctgtttc gccaggacag cctgcagcag
    42661 tttgttattc agtgcatgac cggatttata agcggtaaat gcaccaataa tattgtgacc
    42721 acacatgaac aagtcaccga tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa
    42781 acgcaggccg tcttcgttca gtacgcgata atcgtcaaca acgatggcac aatcgaagct
    42841 gccgcccagg cacaaaccac gggactgcag atattcgata tcacgcatga aaccgaacgt
    42901 acgcgcacgg ctgatctggc gcataaacgc atcagcggag aagttcatcg catagcgctg
    42961 gttgctggaa tcaatagccg gatggttaaa atcgatggtg aaatccagcg aaaaaccatt
    43021 gtacggctta aattcagccc acttatcgcc atcttcgaca cgaacagtct ctttgatgcg
    43081 aacaaatttt ttggcgcagt tcaactcgtc gataccggcg tcaagcagca ggtatacaaa
    43141 cggagcggcg ctgccgtcca tgatcgggat ttccggcgcg ttaacttcga taacaatgtt
    43201 atcgatgccc aggcccgcga gagcagcatt gaggtgctct acggttgaaa tccgtacatc
    43261 atgctcgttg accagacacg tacagagcat ggtatcacgc acagatttgg catcggccgg
    43321 gaaatctacc ggtggattca agtcggtgcg acgatagatg accccggtgt tggccggcgc
    43381 agggcgtaac gtcagagtga ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac
    43441 gatacgttta agtgtccttt gtttgatcat cgtattatct cgccaaatta cctatccaac
    43501 cgaagtgtac tatacattcg gcgggccagt ttagcacaaa gagcctcgaa acccaaattc
    43561 cagtcaattc ttaatcagct tgcttacgca ggaatgctgg gatatccaga taatccggct
    43621 ctttcgcagt ttgcggcgca ttgtcattca cgactttagc aaccggcttc tgctcctggg
    43681 tcagcggagc catcccatgc tgctggtagc gatccatcac tggctgctga acctgcttat
    43741 tggtcaccag agtgatttca ggacgtttgt ccatgccgat acctgtcgca acaacggtta
    43801 cgcgcagctc gtcattcata tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt
    43861 cggaagcaaa tgcacggatg gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga
    43921 agcccgccgt gatgttaacc agcacgccgc gcgcgccaga caggtcgata tcttccagca
    43981 gcggagaaga gatagccatt tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc
    44041 cagaacccat cattgcgtag cccatctcag acattacggt gcgtacgtct gcaaagtcca
    44101 cgttcatcaa acccggacga gtaatcagtt cagcgatacc ttgcacagcg cctttcagta
    44161 catcgttcgc tgcgccaaac gcatccagca gggagatacc gcggcccaga actttcagca
    44221 gtttgtcgtt cgggatagtg atcagagagt ccacatgctt ggacagttca gtgatcccct
    44281 gctccgcgaa tgccatacgc ttcttgcctt caaagttgaa aggcttagtg acgacagcaa
    44341 cggtcaggat acccaaatct tttgccactt cagcgacgac tggtgctgca cctgtaccgg
    44401 taccaccacc catacccgca gcaataaaga ccatgtctgc accttccagc gccgcacgca
    44461 atgcatcgcg atcctcatca gccgcattgc gaccaacttc tggattagcg ccagcgccca
    44521 gtcctttggt gataccgcta ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg
    44581 cttgtgcatc ggtatttacc gcgaagaatt caacaccttc aatacgctcg cgcaccatgt
    44641 gttcaacagc attaccgccg ccgccgccga cgccgatgac tttaatcacc gcgtcattgg
    44701 taagttccat tggttcaaac atagtttctc tccgatttgt gcctgtcgcc tgaggccgta
    44761 atcatcgtcg gcctcataaa aattaaaact cttttcgcag ccaactattg agtcgcttga
    44821 tccacgagcc aactgatgct gtaacacgtt tttctacttc agcttcaccg ttaagatgtg
    44881 actctttccc atagtgaagc aatcccaccg ccgtcgaata atacggctcc tgagcataat
    44941 ccgttaaacc ggtaatgttc agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct
    45001 gagcacaggc tgcaagacct tcgatctgcg ctgcgccacc ggttaataca atgcctgccg
    45061 ccaggtgatg tttaacccct tgttggcgaa gcttttcctg caactgcaat atctcttcgt
    45121 tgaccaggtt gagcagctcg gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt
    45181 gcagactccg tggcggacga ccgcctacgc tcggcacttc cacgctctca tcttttccaa
    45241 cgatggaacc cagcgcacaa ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg
    45301 tgccaaaggc gtaagcgata tcactggtca cgacattgcc agcataagga attaccttag
    45361 tgtggcgcaa tgccccaccg gtataaacgg cgatatccat tgtaccacca ccgatatcga
    45421 cgacgcagac acccagttca cgttcatctt ccgtcaatac cgaataactt gatgccagtc
    45481 cggcaaatat cagttggtca actttcagcc cacaacgttc aaccgctttg acgatgtttt
    45541 tcgccatatc gttgtgacat gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa
    45601 gtcctaccgg attcttgatc ccttcctgat agtcaatcgc atactcttgc gggatcacat
    45661 gcagcacacg atgttcatcg cgcacacgca ccgatttcgc ggtatggacg acgttttcca
    45721 catcttcttg cgtcacttct tcttcagaaa taggcaccat accaatttca ttctggcagc
    45781 tgatgtgctt accagaaagc gccagatata ccgaagagat ctgacaatct gccatcaatt
    45841 ctgcctggtc aatggcgcgt tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc
    45901 ctttatccat accacgtgac gggcagctgc ccacgccaat gatattgacc ataccgtcgg
    45961 gcagaacttc ccctactaaa gcggcaacct tcgcggtacc aatctccagt cctactacca
    46021 gttttctgtc cgtcgccttg atcattgttg ttctgcctgt gcctgatttt gttgctgagt
    46081 agattcctct ggcggcaagg gcgcccagcc tactgccgct ccagagtcat aacgcaaatc
    46141 aacgtagcta atccgtttgc catcggtttg cgcctgctgc tgtaaaaccg gataaagttc
    46201 tacaaagcga gccaaacgtt tcatcgtatc gccccggcca agattgagct taatatcgtt
    46261 attcagcgtc aactgccagg aacgccgcgc ggtcatcgcc gcttccttca gagtaaatct
    46321 gtcctttgcc agcatctgcc ccatttcgcg atagccctgc aacacttcat tggcgctgcc
    46381 ttccgggcca tacagcattg gaagcacctg cttgctggtg cgatctggcg gcacgctgaa
    46441 ggtgtttcct tccgcgtcta ccatatgttg atcattccac cgcgcaatcg gcacatattc
    46501 aaccagatga atcttcaatt catcaggcca ctgctttctg acgctcacct gcttaatcca
    46561 cggcaggcgt tgttctattt gcgtctggat gatgttgaca tcctgggtca taaaggtacc
    46621 cggctcaccc aatgccagga tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc
    46681 accggtcaac accagctttg agagcggcag gcgttgcgca tcttccatcc agcccaacac
    46741 gacccagccg ctcaccaaca ctgtcgttaa aacggtcagc aggaaaagga tccccgccag
    46801 acgcgttcca ttattgcggc gagaagaaac ctcttcttcg ctatttcgcg tgttcagagc
    46861 agcctgcgac atattagtcc gccagttcca gaattcgtac taccaactgc gagaagctca
    46921 tacctgcctg acgtgccgcc atcggcacca ggctgtggct ggtcataccc ggtgaggtat
    46981 tggcttccag cagataaaac tgtccatcgc tgtccagcat aacgtcaata cgtccccatc
    47041 ctttgcaacc taacgtcgtc catgctttca gcactaatgc ctgcaaattg gcctcttgtg
    47101 acgcttccag acctgcgggg cagaaatact gtgtctcatc agagagatac ttcgcctcat
    47161 aatcatagaa ggttccggac ggttgaatac gtattgacgg taaaatttct tcaccgagta
    47221 tcgcaaccgt gaactccggc ccacttagcc atttttcaat caatacttct tcatcgtgct
    47281 gaaatgccaa tcttaatgca tcttgtagag cattttctgc tactactttt gacattccca
    47341 cactggaacc ttcgcggctc ggcttaacga taaccggcaa acccagagca gaaatttctg
    47401 ctaactgctt atcgctcagg cctttttcaa actctgcgcg ggttaacgct acccacggcg
    47461 cgaccggtaa accggcacct tgccacagca gtttgctgcg tagtttatcc attgaaagcg
    47521 cagatgccat cactccgctg ccggtataag gcaagcccat cagctcaagc atgccctgca
    47581 gcgtaccatc ttcaccgccg cgaccgtgta gcgcgataaa cactttctga aaacccatcg
    47641 acttcagttg cgtcacgtcg acttctttcg ggtcgacagg atacgcatca ataccgcctt
    47701 cacgcagccc ggctaacacc gctgcgccag aattcagaga aacttcccgc tcagcggaag
    47761 tcccgcccaa caggaccgca attttatcag tcatgttgtt cttcctccgg agtttgcggc
    47821 ttcagtttga tttcagctaa agaacgggca atttttccga tattaccagc cccctgaacg
    47881 agaatcaggt cgttaccgat taataccggt gccagcatct cggctacctg cgcagggtcc
    47941 ggcaccagaa tgggatcaat tttcccacgt ccacgaattg tgcgacacag cgaacggctg
    48001 tccgctcccg gaattggcgc ttcgccagcc ggatacactt ccagcatcaa cagggtatca
    48061 acctgcgtca gcacattggc gaaatcatca tacaggtcgc gcgtacgggt aaaacggtgc
    48121 ggctgaaaca gcattactag gtttttatcc ggccagcctg cgcgcgccgc tttaatggtg
    48181 gcgtcaactt ccgtcgggtg gtggccgtag tcatcgacca gcattgccgt accgctttta
    48241 ccattcactg gctccagcgg gaattcaccg aggaaatcaa aacggcgacc agtcccctgg
    48301 aagctctcca gcgcacgcaa aatagcctcg tcgtcaattc cctcttccgt agcaaccgca
    48361 accgcagctg cggcgttcag cgcgttatga cggcctggcg cattcagggt gacgcgcatc
    48421 ggctctttgt cctggcgcag cagcgtaaag tgcccctgcg ggccaatctg ctgataatct
    48481 tctacacgca cgtcggcatc ttcgctgaag ccgtaagtcg tggtctgacg tcccacacgc
    48541 ggtaacaatt cgcggatcac cggatcatca acacacatca ccgcacgacc gtaaaacggc
    48601 aggttgtgca gaaaattaat aaaagtctgt tttaaattct caaagtcgcc ctggtaggta
    48661 tccatgtggt cggcttcgat attggtgaca atcgccacca tcggttgcag atgcaggaac
    48721 gatgcatcac tctcatctgc ttcggcaatc aggtaccgac catgccccaa acgcgcatga
    48781 acccccgccg cttttaccag cccgccgtta acgaaggttg ggtcgagccc cgcttctgcg
    48841 tagatgctgg aaaccatcgc ggtggttgtc gttttgccgt gcgttcctgc aatggcgatg
    48901 ccatgacgaa aacgcattaa ctcagccagc atttcggcac gacggatcac cggaatacgc
    48961 gcttcatgag ctgcgacaat ttccgggtta tcggcagaaa tggcgctgga aacaacgacc
    49021 acgctggcat cacgtacgtt ttccgggcga tggttgaaat aaatcgtcgc tcccagattc
    49081 attaactgct gcgtgaccgg atttggcgct aaatcggaac cactgatctg ataaccttca
    49141 ttggccagaa cttcggcaat accgcccata ccggcaccac cgatgccgac aaaatgtatg
    49201 tgccgaacgc gacgcatttc gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc
    49261 attctttacg ccattaactt cttaaattca tacgatgcaa aaggcatcgc tacaattacg
    49321 cccgggcagc ccggctcact tcatttgcca ctcgctcggt ggcatccgga atggatgcag
    49381 cgcgggcgcg ttctgccatg gttaataagg tttctcgcga ccacccggcc agggtgttgg
    49441 cgacagcatc cacgctaagc tgtggctgct cgataatttt ggctgcgccc gctttttcca
    49501 gcggtagcgc attccagtat tgctggcggt ctttatgttg aaacggcaca aacaacgccg
    49561 gtagtcctgc cgcggcgatt tcactcaccg ttaaagcacc ggagcggcaa acgacgacat
    49621 ccgcccacgc atacgccgcc gccatatcat caataaattc cgtcacttta tgctgcggtt
    49681 gccccgcttc ggcatacgcc tgttcaacgg attgttgcga acctttaccg ctctgatgcc
    49741 agatagtgac tgaatcaccc agtttcgcag caacctgcgg cattgtctgg ttaagaatgc
    49801 gtgcgccctg ggaaccaccc actaccagca cacgaaccgg accttcacgt ccagccaaac
    49861 gttgctgcgg caacggcagc gccagcacat cggtacgcac cgggttaccc accacttccg
    49921 cattagggaa agcgcctgga aacgcctgca tcactttggt ggcaatcttc gccagccatt
    49981 tattggttaa gcccgcaata ccgttttgtt catgaagtac aaccggaatg cctaacgacc
    50041 acgcggccag accacctgga cctgatacat agccgcccat accgagtacc acgtcaggtt
    50101 tgtacgcttt cataatcgcc cgcgcctgac gccaggcgtt gaagatacgc agcggcgcag
    50161 ctatcagtgc ttttatacct tttccacgca gaccagagat acgaatgaaa tcaatttcga
    50221 tgccatgttt tggcactaag tccgcttcca tacggtcggc agtccccagc cagcgaactt
    50281 gccaaccctg agccattaga tggtgcgcaa ccgccagtcc cgggaataca tgtccaccgg
    50341 ttccgcctgc catcaccatt aatcgctttc cttgaccact catcgtgaac ctcgtacaaa
    50401 cgcctgcgct ttctccagac gcgtttcata atcaatacgc aacagcatca tgatggctgt
    50461 cgacataatc agtaagctcg aaccaccgta actgatcagc ggcaatgtca gacctttggt
    50521 cggtaacatc cccgccgccg cgcctacgtt aaccagcgcc tggaagctaa accagatgcc
    50581 aatagaacag gcgagaaaac cggaaaaacg gtggtcaatt tctaacgctt tgcggccaat
    50641 cgacatcgcg cgaaaagcga cgaagaatac cattaaaagt gccagcacca caccgacata
    50701 ccccagttct tcgccgataa tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc
    50761 cagtttttgt accgagttac ctaaaccttg tccccaaagt tcgccgcgac caaacgccat
    50821 cagcgattgc gttaactgat agccgctgcc aaagggatct tcccacgggt tccagaatgc
    50881 ggtaacacgg cggatacggt acggttcggc gagtatcagc aacacaaccg ctgaaatgcc
    50941 cataccgata atggcaatga actgccacaa tttcgctccc gccaggaaca acatcgccag
    51001 cgtagtcaca aacaacacca ccaccgtacc aaggtctggc tgtgccagca gtaacactgc
    51061 caacaccaga atcacgccca tcggtttcag gaagccgcgc aggttattac gtacttcgtc
    51121 gcctttacgc accagatagt tggcgatata acaaaacagc gacagttttg tcagctccgc
    51181 aggctggata cgcagcaaac cgagatcgat ccaacgcgat gcccctttaa ccgagctacc
    51241 cactaccagc acgatcatca gcaggatgat agatccgagc agcatcgtgg cactgtagcg
    51301 ttgccagaac tccatcggca gacgcagcgt aatgatcgcc agaatgaatg ccaggatcag
    51361 atagacgcca tcacgcttcg cgaagaagaa cggatcgttg gttaagcgtt gccctatggg
    51421 cattgacgcc gaggtcacca tgataaagcc aatcgccgca aggccgaagg tcagccacag
    51481 taaggtgcga tcgtacatga tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca
    51541 gccttttagc gccgtggaga tccagaccag gatactgaat cctggcaggc gcggcatttt
    51601 caggcgaggg agagataaac gcatcaacct aactccttcg ccagacgggc aaactcattg
    51661 cctcgttgtt caaagttctt gaactgatca aggctggcac aggccgggga gagcagaacc
    51721 atatcgccca gctgaacacg cggagccagc aagcgcatcg cttgttccat ggtttcggtt
    51781 tgttctgcca cttccgggcg tagcgccgcc agctgcgcac cgtcacgacc aaaacagtac
    51841 aaacgcacgt tgtcgccatt cagatagcgc gccagtggac tgaagtccgc cgatttacca
    51901 tcgccgccca gcaacaaatg cagcgtgccg tctacctgca agccattcaa cgccgcttcc
    51961 gtactgccga cgttagtggc tttcgaatcg ttaatccagc gtacgccatt gtgctccagc
    52021 accacttcaa agcgatgcgg cagaccggtg aaggtggtta acgctttcag gctgctggcg
    52081 cgcggcaaac cagctgcgtc tgccagtgcc agtgctgcca gcgcattggt gtagttatgc
    52141 tgcccggaaa gtttcatctc tttcacattc agcactttct cgcctttaac ccgcagccag
    52201 gtttcgccct gctgatgatt caggtgatag tcacccatgt tgacgccaaa gctgacgcag
    52261 cgttcatcag caccgcgaat cggcattgtt aaggcatcat cagcattgac gacacaaact
    52321 ttcgcatttt cgtaaatacg caattttgcc gcacggtatt gctgcaaacc aaacggatag
    52381 cggtccatat gatcttcagt cacgttcaga atggtcgccg ccaccgcctg taagctggaa
    52441 gtggtttcca gctggaagct cgacagttcc agcacgtaca gttcgcactc agcatcaagc
    52501 agcatcaacg ctggcaggcc aatattgcca cccacaccaa cgttaacccc cgccgctttc
    52561 gccatttcac ccactagcgt ggtgaccgtg cttttgccgt tagaaccggt aatcgccaca
    52621 atcggtgctt gtgcttcgcg acagaacagc tcgatatcgc caacgatttc gattccggca
    52681 tcagcggcag cgcttaagga tggatgcgcc tgtgcaatac cgggactggc gacaatcaga
    52741 tctgccgcca tcagccattc atcattcaga ctgcccgtgt ggcgttctac ggcttcgggt
    52801 aatttatcca ggccaggcgg tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga
    52861 gcgaggaaaa agtccacgca ggaaagcccg gtgaggccca ggccgataat gacgacattt
    52921 ttaccctgat aatcagccat gattaacgta ccttcagcgt tgccagacca atcagaacca
    52981 gcatcagcga aataatccag aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt
    53041 catagtggtg atgaatcggt gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc
    53101 cgacctgcag gatgacagaa agcgtttcta ccacgaacac gccccccata atcaccagca
    53161 ggaattcctg acgtagcagt acggcgataa tgcctaacgc accacctaac gccagcgaac
    53221 ctacatcgcc cataaagacc tgcgccggat aggtgttaaa ccacaggaag cccagtcctg
    53281 ccccgactat cgccgtgcag acaatgacca gttcccctgc gtgtcgcaga tacggtatat
    53341 gcaagtagct ggcaaagttc atattgccgg tcgcccacgc caccagcgca aaaccaccgg
    53401 cgacaaatac ggtcggcata attgccaggc cgtcgagacc atcggtcagg tttaccgcgt
    53461 tgccagtacc cacaatgacg aagtaagcca gcagaatgta gaacagcccc agctgcggca
    53521 tcacatcttt aaagaatggg accaccagct gcgttgcggg cgtgtctttg ccggcaaggt
    53581 acagggcgaa ggcgacaccc agcgcaatga ccgacatcca gaaatacttc caacgagcga
    53641 tcaacccttt ggtgtcttta cgcaccactt tgcgataatc atcaacaaag ccaataacac
    53701 cgtaacctac cagcaccacc aacacgcacc agacgtacgg attggacggg taagcccaca
    53761 gcagtacgga gatcacaatc gccgtcagga tcataatccc gcccatggtc ggcgtaccgc
    53821 gcttgctgaa gtgtgattca ggaccgtcgt tacgcaccac ctgaccaaag gaaagttttt
    53881 gcaaatgagc tatcatacgc gggcccatcc acaatgagat gaacagcgcg gtcagcaggc
    53941 tgacgatggc gcgaaacgtc agataggaaa agacgttaaa gccggaataa tatttgacca
    54001 aatgttcggc cagccaaact aacatgtccc attctcctgt aaagcgcgta ctacctcttc
    54061 catggcggca ctacgtgaac ccttaactaa aatcgtaatt acctgttgct cagcaatcag
    54121 tgatttaaga cgcgtaatta acgcagtttt atcagcaaaa tgttcgccaa cgccgctggc
    54181 ggtgctgata gcatggcttt gtttacccac gcttaacacg cggtcaatac cagcagcttt
    54241 tgccgcctcg cccacctgta catggcaggc ttcgctttca gcgcccagtt ccgccatatc
    54301 gcccaccacc agcacgcggt agcccggcat ttcagccagt acctgaacag cggcggtcat
    54361 tgaaccgaca ttggcgttgt aggagtcgtc gagcagcaac tggttttctg ccagttggat
    54421 ggggaacaga cggcctggaa cagctttcag atttgccagc cccgctttga tagcatcaag
    54481 cgttgcgccc acggacatgg agagcgcagc ggctgccagc gcattcgcaa tattgtgacg
    54541 ccccggcaac ggcagcagaa catcgacgct accggttggg gtttgtaggg taaattccgt
    54601 accgtgcgag gtcacatgga tattggtggc ggtgaaatcg ctgttggcgg cattgggtga
    54661 gaaacgccac actttgcgtg agccaattac gctctgccag ttcagccagt cgttgttgtc
    54721 ggcgttcata atggcgatac cgttttccgg caggccgcta aagatttcac ctttcgcttt
    54781 cgcgacaccc gcaagcgagc caaaaccttc cagatgcgcc gctgccaggt tgttgaccag
    54841 cgcagcttcc gggcgagtca gactcacagt ccaggctatt tcgccctgat ggttcgcgcc
    54901 aagttcaata actgcgtaat cgtattccgg cgtcaagcgc aacaacgtca tcggtacacc
    54961 gatgtcgttg ttgagattgc ctgccgtata aagcgtgttg ccgcactggc ttaaaatcgc
    55021 cgccgtcatc tctttaacgg aggttttgcc ggaggaacct gtcagagcaa ccacgcgcgc
    55081 cggaacttgc tggcgaaccc atgcagccag ttcaccaaac gccagacgcg tatccttgac
    55141 gattaactgc ggcaggtcga tatccagcgg acggctgacc agtagtgcgc ccgcgccgcc
    55201 agctttcgcc tggtcggcaa aatcatgagc atcgaaacgt tcgcctttca gggcaacaaa
    55261 caggcagccc ggcgtcagtt ttcgggtatc agtggttaca gcatcaaggg tgatatctgc
    55321 accttgcagt tcaccgttga gaatgtcggt aagttggcta agggttacgc taatcatgca
    55381 atcaccccca gcagacgcgc caccgtgacg cgatcggagt agtccagacg ctgattgcca
    55441 acaatctggt aatcttcatg gcctttgccc gcgaccagta ccacatcatt ctctttagcc
    55501 tgcataacgg cgcaagtcac cgcttcagca cggccttcca tcactttggc atgtccggca
    55561 tctaacattc ccgccagaat atcgttgatg atggcacgcg gttcttcggt acgcgggtta
    55621 tcgtccgtca ccaccgccac gtcagcaaac tcttcggcaa ttgcgcccat cagtggacgc
    55681 ttacctttat cgcgatcgcc accacagcca aagacgcacc acagcttgcc cgcacagtgc
    55741 aggcgcgccg cctgtaaggc tttttccagt gcatccggcg tatgcgcgta atccaccacc
    55801 accgtcggtt tgcctggcgc agtgaacact tccatacgtc cgcaaaccgg ttgcagacgc
    55861 gcggcggttt tcagcagatc agccagtgga tagccgagtg ccagcagtgt cgccagcgcg
    55921 agcagcaggt tgctgacgtt aaaagcgccc atcagatggc tttcaatttc gccatcgccc
    55981 caacttgagc taaagcgaat cgtcgcaccg ctgtcgtgat agttcacgtc gatcgctttt
    56041 aaccagcgtc cgtgacagtt cggattaata tgatcttcca ttgataccgc aaccgcgtcc
    56101 ggcagttttg ccagccagcg gcggcccact tcatcgtcgg cgttaataat cgcctgaccg
    56161 caatgatgtt cagaataaag cagccatttc gcagcttcgt agtgttccat atcaccatga
    56221 taatcaaggt gatcgcggct taagttggta aagaccgacg ccgcaaattt caatgccgcc
    56281 acacggtgct gtaccagccc gtgggaggaa acttccattg cgcaaaacgt cgcgccctgc
    56341 tccaccagcc ccgccagctc atgctgaaca tcgactgcgg aaccggtagt attttctgtc
    56401 gggatcactt tcccaagcag accgttacca acggtgccca ttaccgcgct ggtttcgcca
    56461 agcagttggc tccactgcgc cagcagctgg gtagtcgtgg ttttgccgtt ggtgcccgtt
    56521 acgcctacga gacgtaaatt gtcagagggt tcatggtaaa agcggcccgc cagtgcagat
    56581 aaacgctcat tgagctggct gagatagatg accggtacgc cgtgcatttc acggatttca
    56641 ccgtcggtcg cctcatcttt cgctttcgcc tctgcaataa tggcagccac accttgcgct
    56701 atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac ctactacagc tacaaagaga
    56761 tcgcccgccg cagccacacg gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg
    56821 tctggcaccc acggagcaag aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc
    56881 ttgattaatc acaaattcat ttttatcgcc cgttgtcagc gcatccggct cgatgttcat
    56941 ggtacgcaat acgccgccca tgatggcacc aaagaccggc gcggaaacgg cgccgccgta
    57001 gtatttaccc gcctgcggat cgttgataac aacaaccagc gcgaagcgcg gctgactcgc
    57061 aggcgcaacg cctgcggtat aagcaatata tttattgatg tagcgaccgt ccggcccgac
    57121 ctttttcgcg gtaccggttt taatggcgat acgatagcct ttaatcgccg ccttcacgcc
    57181 gccgccgcct ggtagcgcca cgctttccat catatgcacc acagtgcgga caatggatcc
    57241 cgggaagaca cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca gtgggcgata
    57301 aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag
    57361 cccgtagccg aaagagaagg tggccctctc tatgtcagac caccgttgtt tttgaggata
    57421 taagccactg cgttctccga ccaaccccaa attggtcgct tttcccagtc caaaacgtga
    57481 gtaagtatct actaacgctg aggacggcat cgctaacgcc agcttggaaa caccgacgtt
    57541 actcgacttc tgtaataccc cggtcagggt taattcgctg tagcgtgcca cgtctttgat
    57601 ttcgtggccg ttaattcgat aaggaatggt attgagtacc gagttttccc gcaccacgcc
    57661 acgttgcaac gcggtcatta ccaccatcgg tttaaccgtt gagcccggtt caaacacgtc
    57721 ggtgatggta cggttacgca tcgcctcttt cggcgtgccg ctcagattgt tagggttgta
    57781 tgacgggctg ttagccatcg ccagcacttc accggtgttg acatccacca gcacggcgct
    57841 accagattca gccttgttaa aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg
    57901 caggcgttca tcaatactca gcgccaggtt gtgcgctgcc tggctgtcag tagaagaaat
    57961 atcttcaatt acgcgaccat agcggtcttt acgcacaatg cgctcacccg gctgcccggt
    58021 aagccattta tcgaaactct tctcaacgcc ctcaatccct tgactatcga cgttagtaaa
    58081 gccgatgagg tgagcagtca cttcgccgga cggatagtaa cggcgagact cttcacgcag
    58141 atgaatcccc ggcagtttca gttttttgat gtagtccgcc atgtcagggt tcacctgacg
    58201 cgccagataa ataaaacgcc ctttcgggtt ggcgttaatg cgggctgaaa gctgatccag
    58261 cggaatattg agcgcgttag ccagcgcctt ccagcggtca ccgacgctga taccgccagc
    58321 gtcatgcact tctttcgggt cagcccaaat cgcttttacc ggcacgctca ccgctaacgg
    58381 gcgaccagaa cggtcagtaa tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga
    58441 acgcatgtcg ccctctttca ccagcatatc cggggagata acttgtaacc acgctacgcg
    58501 tccgagcaga aaagccagcg ccaggagaat acagccgcat aacaacgcaa aacgccaact
    58561 gataaagttg gcatgttctt cctgacgttt tggtttctgc gttttcgccg ctgctttcat
    58621 gcgtcgcgtt tatccttatt tttgcactac gatattttct tgtgacggat caacatgctg
    58681 catttgcagc ttttccgtgg cgatcctttc cacccggcta tggtcgccga gcgcattctc
    58741 ttcaaggatc aggttgcgcc attcaatgtc taaagcatct cgctccagca ccagttgttc
    58801 gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt accacagtca ccgccgtcaa
    58861 aataatgcaa atgaacaggc agagtggcag cttcccaaat cgcaaaagat cgtcaccgat
    58921 aacaccaggc aatgcatggc gctcgtggct tcccatcgat cctttaactt tgcttagagc
    58981 ttctgtcact ctgctgatca tgcattcgtc ctctctgcaa tacgcagaac tgaactacgg
    59041 gcacgagggt tctcagccac ctcttcttcg cccggcatta acttgcctag tgctcgcagc
    59101 tgacggccac ccagtttttt gagctgctct tcagtcatcg gtaaccctgc cggaacttgc
    59161 ggaccgcggc tgttttcacg cataaaacgt ttcacaatac ggtcttccag cgagtggaag
    59221 ctgatgatcg aaagccgccc acccggggcc agcacgttga gcgagctttt tagcgcctgc
    59281 tctatctcct ccagttcact gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg
    59341 ggatgtttaa acttatcttt caccggcgtt gcaacagcca cgacttccgc cagttctttg
    59401 gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg cgcgggcaat gcgtttggca
    59461 aaacgctctt caccataggt tttcaatacc caagcgatat cggcttcttc tgcggtttgt
    59521 agccattcag cggctgactg cccacgggtt gggtccatac gcatgtccag cggaccatcg
    59581 cgcataaagg aaaagccgcg ctcagcatca tcaagttgcg gtgaagagac gccaagatcg
    59641 aggagaatgc cgtcgatctt gccgataaga tcgcgctcgg caacgtattc gcccagcgcg
    59701 gagaaaggtc cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata
    59761 gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg cgagaggatc
    59821 agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt agatgccatc aggacggata
    59881 ttgaggccat taacggcttc atccagcagc accgtagtat gtttatagtt ttccatcatt
    59941 ttatagagac aagtcctgca gtcgctccga taagtctccg gtagccaact gctctgcgtc
    60001 gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa acttgttgaa
    60061 ctgtccaacc agcatcactt cttttgtcag cccggcatgt tgccgcagta ctggcgcgat
    60121 taacaatcga cctgcgccat ccatctgaca ttcgctggca tgacctaaca gtaggcgctg
    60181 cacacggcgc tcaaccgggt tcatgctcga cagacgcgat aatttttgct cgataatttc
    60241 ccattcaggc agggggtaaa gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat
    60301 ttgaccgaca gcgttctcaa gcagctgttc ccggtaacgg gtaggcactg ataagcgccc
    60361 tttgctgtcg agattgacta acgttgctcc ccggaacatg ccagcctcac cccttattcc
    60421 cactttgccc cacaatttcc cactgaaagg agtttacgga gcggaggaaa agcttgtcaa
    60481 gccagtcaca aggcataccg aggtaaaaaa cccgatgttt acggctaata tcagcgttgt
    60541 ttattttacg tccaacgtgc gcagcaaatt aacgttatga atagctgtaa gaaaaaatcc
    60601 ctcatcgtca tcaccgtttt aaaatgactc actattttcc acagcaaata taaagtgtca
    60661 gtttgcgacg cgagcggcat tttaggacat atcttccccg gttaacagtc cctgttgcgt
    60721 ctgtgtggcg cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgc ttgttaaatc
    60781 ggcaattcat ctctgcatgt aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat
    60841 tattgtgaat ttcagctggt gagttaatta acgggcgggt ttaaaagaac ttatcaataa
    60901 tagtcaggaa tagtcttatt tactttaagg atattgatgt ccattcccgt actctacg
//
LOCUS       DAETQN010000009        59841 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.009, whole genome shotgun sequence.
ACCESSION   DAETQN010000009 DAETQN010000000
VERSION     DAETQN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.009"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..262)
                     /locus_tag="K0W68_000607"
     CDS             complement(<1..262)
                     /locus_tag="K0W68_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBI3072265.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
     gene            369..665
                     /locus_tag="K0W68_000608"
     CDS             369..665
                     /locus_tag="K0W68_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3072266.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(799..1224)
                     /gene="arsC"
                     /locus_tag="K0W68_000609"
     CDS             complement(799..1224)
                     /gene="arsC"
                     /locus_tag="K0W68_000609"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBI3072267.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="K0W68_000610"
     CDS             complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="K0W68_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBI3072268.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="K0W68_000611"
     CDS             complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="K0W68_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBI3072269.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(3472..3645)
                     /locus_tag="K0W68_000612"
     CDS             complement(3472..3645)
                     /locus_tag="K0W68_000612"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072270.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            3673..3756
                     /locus_tag="K0W68_000613"
     CDS             3673..3756
                     /locus_tag="K0W68_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBI3072271.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="K0W68_000614"
     CDS             complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="K0W68_000614"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBI3072272.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="K0W68_000615"
     CDS             complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="K0W68_000615"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBI3072273.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6279..8321
                     /gene="prlC"
                     /locus_tag="K0W68_000616"
     CDS             6279..8321
                     /gene="prlC"
                     /locus_tag="K0W68_000616"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBI3072274.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8329..9081
                     /gene="rsmJ"
                     /locus_tag="K0W68_000617"
     CDS             8329..9081
                     /gene="rsmJ"
                     /locus_tag="K0W68_000617"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBI3072275.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="K0W68_000618"
     CDS             complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="K0W68_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HBI3072276.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="K0W68_000619"
     CDS             complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="K0W68_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBI3072277.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11742..12077
                     /gene="uspB"
                     /locus_tag="K0W68_000620"
     CDS             11742..12077
                     /gene="uspB"
                     /locus_tag="K0W68_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBI3072278.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="K0W68_000621"
     CDS             complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="K0W68_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBI3072279.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14052..15254
                     /locus_tag="K0W68_000622"
     CDS             14052..15254
                     /locus_tag="K0W68_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBI3072280.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15570..16553)
                     /locus_tag="K0W68_000623"
                     /pseudo
     CDS             complement(15570..16553)
                     /locus_tag="K0W68_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            16953..18560
                     /locus_tag="K0W68_000624"
     CDS             16953..18560
                     /locus_tag="K0W68_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBI3072281.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            18822..20444
                     /locus_tag="K0W68_000625"
     CDS             18822..20444
                     /locus_tag="K0W68_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBI3072282.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            20810..21877
                     /locus_tag="K0W68_000626"
     CDS             20810..21877
                     /locus_tag="K0W68_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBI3072283.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            21874..24609
                     /gene="rbbA"
                     /locus_tag="K0W68_000627"
     CDS             21874..24609
                     /gene="rbbA"
                     /locus_tag="K0W68_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBI3072284.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            24609..25733
                     /locus_tag="K0W68_000628"
     CDS             24609..25733
                     /locus_tag="K0W68_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3072285.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            25806..26079
                     /locus_tag="K0W68_000629"
                     /pseudo
     CDS             25806..26079
                     /locus_tag="K0W68_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            26076..26435
                     /locus_tag="K0W68_000630"
     CDS             26076..26435
                     /locus_tag="K0W68_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBI3072286.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="K0W68_000631"
     CDS             complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="K0W68_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBI3072287.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="K0W68_000632"
     CDS             complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="K0W68_000632"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBI3072288.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="K0W68_000633"
     CDS             complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="K0W68_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBI3072289.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="K0W68_000634"
     CDS             complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="K0W68_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBI3072290.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="K0W68_000635"
     CDS             complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="K0W68_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBI3072291.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="K0W68_000636"
     CDS             complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="K0W68_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBI3072292.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="K0W68_000637"
     CDS             complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="K0W68_000637"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBI3072293.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(32630..33679)
                     /locus_tag="K0W68_000638"
     CDS             complement(32630..33679)
                     /locus_tag="K0W68_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBI3072294.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            33811..35028
                     /locus_tag="K0W68_000639"
     CDS             33811..35028
                     /locus_tag="K0W68_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3072295.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="K0W68_000640"
     CDS             complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="K0W68_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBI3072296.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(35662..36327)
                     /locus_tag="K0W68_000641"
     CDS             complement(35662..36327)
                     /locus_tag="K0W68_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBI3072297.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            36548..36793
                     /gene="tusA"
                     /locus_tag="K0W68_000642"
     CDS             36548..36793
                     /gene="tusA"
                     /locus_tag="K0W68_000642"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBI3072298.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="K0W68_000643"
     CDS             complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="K0W68_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBI3072299.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(39167..39793)
                     /locus_tag="K0W68_000644"
     CDS             complement(39167..39793)
                     /locus_tag="K0W68_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBI3072300.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            39934..40293
                     /locus_tag="K0W68_000645"
     CDS             39934..40293
                     /locus_tag="K0W68_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBI3072301.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(40296..40565)
                     /locus_tag="K0W68_000646"
     CDS             complement(40296..40565)
                     /locus_tag="K0W68_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBI3072302.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="K0W68_000647"
     CDS             complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="K0W68_000647"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBI3072303.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            41301..42797
                     /gene="ftsY"
                     /locus_tag="K0W68_000648"
     CDS             41301..42797
                     /gene="ftsY"
                     /locus_tag="K0W68_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBI3072304.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            42800..43468
                     /gene="ftsE"
                     /locus_tag="K0W68_000649"
     CDS             42800..43468
                     /gene="ftsE"
                     /locus_tag="K0W68_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBI3072305.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            43461..44519
                     /gene="ftsX"
                     /locus_tag="K0W68_000650"
     CDS             43461..44519
                     /gene="ftsX"
                     /locus_tag="K0W68_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBI3072306.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            44764..45618
                     /gene="rpoH"
                     /locus_tag="K0W68_000651"
     CDS             44764..45618
                     /gene="rpoH"
                     /locus_tag="K0W68_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBI3072307.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            45889..46992
                     /gene="livJ"
                     /locus_tag="K0W68_000652"
     CDS             45889..46992
                     /gene="livJ"
                     /locus_tag="K0W68_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBI3072308.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(47191..47574)
                     /gene="panM"
                     /locus_tag="K0W68_000653"
     CDS             complement(47191..47574)
                     /gene="panM"
                     /locus_tag="K0W68_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBI3072309.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            47998..49107
                     /gene="livK"
                     /locus_tag="K0W68_000654"
     CDS             47998..49107
                     /gene="livK"
                     /locus_tag="K0W68_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBI3072310.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            49155..50081
                     /gene="livH"
                     /locus_tag="K0W68_000655"
     CDS             49155..50081
                     /gene="livH"
                     /locus_tag="K0W68_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBI3072311.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            50078..51355
                     /gene="livM"
                     /locus_tag="K0W68_000656"
     CDS             50078..51355
                     /gene="livM"
                     /locus_tag="K0W68_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBI3072312.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            51352..52119
                     /gene="livG"
                     /locus_tag="K0W68_000657"
     CDS             51352..52119
                     /gene="livG"
                     /locus_tag="K0W68_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBI3072313.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            52121..52834
                     /gene="livF"
                     /locus_tag="K0W68_000658"
     CDS             52121..52834
                     /gene="livF"
                     /locus_tag="K0W68_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBI3072314.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            53118..54182
                     /gene="ugpB"
                     /locus_tag="K0W68_000659"
                     /pseudo
     CDS             53118..54182
                     /gene="ugpB"
                     /locus_tag="K0W68_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            54280..55167
                     /gene="ugpA"
                     /locus_tag="K0W68_000660"
     CDS             54280..55167
                     /gene="ugpA"
                     /locus_tag="K0W68_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBI3072315.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            55164..56009
                     /gene="ugpE"
                     /locus_tag="K0W68_000661"
     CDS             55164..56009
                     /gene="ugpE"
                     /locus_tag="K0W68_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBI3072316.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            56011..57081
                     /gene="ugpC"
                     /locus_tag="K0W68_000662"
     CDS             56011..57081
                     /gene="ugpC"
                     /locus_tag="K0W68_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HBI3072317.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            57078..57821
                     /gene="ugpQ"
                     /locus_tag="K0W68_000663"
     CDS             57078..57821
                     /gene="ugpQ"
                     /locus_tag="K0W68_000663"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBI3072318.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(57808..58248)
                     /locus_tag="K0W68_000664"
     CDS             complement(57808..58248)
                     /locus_tag="K0W68_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBI3072319.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            58368..>59841
                     /gene="ggt"
                     /locus_tag="K0W68_000665"
     CDS             58368..>59841
                     /gene="ggt"
                     /locus_tag="K0W68_000665"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBI3072320.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT        14594 a        15113 c        16139 g        13995 t
ORIGIN      
        1 acccggcttt cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg
       61 gggtaacggc tcccaacaga gcagccagga gatagcctct cccttttctg gcccccatca
      121 tctgctggat tttatggtct ggcacctttt gtcttatcag gctgacaccg gcactaatca
      181 ctataaacag caacgaaagc tcgacggcga gaaacacaaa catctctgcg gcatcttgca
      241 gcatcgcaag ccaactactc atacgtaacc tcattggata aatctatatt tccagaataa
      301 tcgaaatgaa attcagatca agatatttct ggtattatcg aaatatcaac cagacagagg
      361 ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa agagctaggt cacccgaccc
      421 gtttgttcat atttaaacat cttgtcaaag ccggggaaca agggttgccg gtcggtgagt
      481 tgcaaaagca gcttgggata cctggctcca cactcagcca ccatatatca gcactggtat
      541 cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct gatgtgtgtt tcgcaatacg
      601 caatgctgga agcgataatt gaattcttac gcgaggagtg ctgcgtaaat agcaaaattg
      661 cctgatgcgc tacgctctac atatttcatg ccatatattg aatttgcacg gttttgtagg
      721 tcggataaga cgcgttagcg tcgcatccga cataaacaaa gcgcacttta ccaactatcg
      781 gcagtggata tcgccgcttt atttcaggcg cttacccgct tcatcaacca ctttctcgcc
      841 atcttccttg gagaatgcgc ctttttgcgc atctggcaga atttccagca ccacttctga
      901 agggcggcac aggcgagttc ccagcggcgt caccacaatc gggcgattaa tcagaatcgg
      961 gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat ttatcttccg caaggcccag
     1021 ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt acggaaatcc ccatatcggc
     1081 aatgagtttg accagttcat cgcgcgttgg cggagtttcc agataatgga taatggtcgg
     1141 ttctgtgccg ctgttgcgga tcatctccag cgtattacgc gacgtgccgc aggccgggtt
     1201 gtgataaatg gtaatgttgc tcatatcagt atctcattac aaagtgaaag agagacgtag
     1261 cgccagcgca gccagcgtca caaacagcac aggcagggtc atgataatcc ctgtacggaa
     1321 gtaatatccc cagctgatag tcatattctt ctgcgaaagt acgtgcagcc agagtagcgt
     1381 agccaggcta ccaattgggg taattttcgg tcccaaatcg cagccaatca cattggcata
     1441 aaccatcgct tctttgataa cgccagatgc cgtgctgcca tcaatggaca acgcgccaac
     1501 cagtaccgtc ggcatattgt tcataataga agagaggaag gcggtgagga atccggtgcc
     1561 gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg agtacgccag aaagatattc
     1621 cgttaatccg gcattgcgca ggccataaac caccagatac atgccgagcg agaagatgac
     1681 aatctgccag ggggcaccgc gcaggacttt acccgtatta atcgcatgac cgcgtttagc
     1741 gacgacaaat aatatcagcg cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg
     1801 ttccaggacg aaaaatccca ccagcagaag cagtaaaaca acccagccag ttttgaacgt
     1861 agcaggatct ttgatcgctt ctgcgggaga tttcagcagc gccatatcgt agttctgcgg
     1921 aatatctttg cgaaaataga gatgtagcat caccagcgtg gcaacaatcg cggcgatatc
     1981 caccggcacc atcaccgagg cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac
     2041 gatattcacc aggttggaga caataagcgg caggctggcg gtatcggcaa tgaatccggc
     2101 cgccatcacg aacgccagcg tcgtgccttt actgaatcct aaagccagca gcatggcgat
     2161 gacaatcggc gtcaaaataa gcgccgcgcc atcattggca aacagggcgg caacggcagc
     2221 accgagcagg acaatccagg taaacagcaa gcgaccacga ccattacccc agcgtgagac
     2281 gtgcagcgcc gcccattcaa aaaagccgga ctcatccagc agcaggctga tgataatgac
     2341 ggcgataaac gcagccgtcg cgttccagac gatattccac accaccggaa tatcacccgg
     2401 atgaaccacg cccgtaacta acgccagtac tgcgccgagc gttgcactcc agccgatgcc
     2461 taaacctttc ggctgccaga taaccaatac gatggtcagg acaaagatag cgcctgccag
     2521 taacataatg cctcccggat aaaacacatc tgaaaattca tatgtgttta gctaaatttt
     2581 taactgcaaa tgttcttact gtccccggaa cagttttgtc gagccaggtt gcggacaatc
     2641 gcctgaacct tttcctgttc acatcgccag gcctcatcaa taattttcgc cgcccatgct
     2701 ggaatatgcg gtgataagcg gtaatgaacc cacttacctt gcttgcggtc cagcaatagc
     2761 ccgctttcac gaagcaatgc caggtggcgg gagatcttgg gctgcgactg atcgagggca
     2821 gtacaaagat cgcagacgca taactctccc agctcgctga gcagtaaaac gatgcccaga
     2881 cgggtttcat cagcaagaat tttgaacaat tggatgggta acagaaatga catattgcgc
     2941 tcctgattgt ggcaggtagt gtctctcttc gaagcggata agtcaaaaac atatatgact
     3001 taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa ttttgagttg gctattcatt
     3061 tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt gcgtcggttt tttactcttc
     3121 agcctgagtt ggtggctctg gctggagtga gaatgcccac atctaacggt tcaatactat
     3181 tagccagtgg ctaacattgc aagaattagc gatttcttca gctggcggag ggggcacttc
     3241 ggtatatatc gcaatgacga tttatatact ttcactgggt catcgtcata ttaagccttg
     3301 tccgaatccg gatgtaaaaa aaccgacttt gcgtcggttt ttttactctt cagccctgag
     3361 ttggtggctc tggctggagt gagatagtca tcatctaacg catcaatgct aataccatac
     3421 tgaaactatt gcaaggacat gctggtttta taacctgaat gtactgtatg attatccagt
     3481 tagctctgag gcattttcac tctggtaatg cgcataaacg ctttcaaagt cctggtcaga
     3541 agtacgggtg gtgccgttaa ctgatgctct ggccggagtg agatagtcgt catctaacaa
     3601 tgagacatgc gccgtgacag gcagtggatg agtaagcgga tgcattctca ctccatcgca
     3661 tggagaaaac gggtgattga taaagcaatc atcgttcttg gagcgttaat tgcgctgctg
     3721 gaactgatcc gctttctgct tcagcttctg aactgatagc ggaaacgtaa ttaagggcta
     3781 agagcacact actcttagcc ctttaacatt tagcgcatgg tcacgaactc ttctgccgcc
     3841 gttgggtgaa tggcgacggt attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc
     3901 accgcgaagc cctgcaacat ttcgtccata ccaaagccaa tgccgtgaat accgacaatc
     3961 ttctcttctg gcccaacgca caccagcttc atgcggcacg gctggcggtg agtggtgacg
     4021 gcggtataca tcgcggtgaa agaggattta tacactttca cctgatcgtc gccgtactgc
     4081 tcgcgcgcct gcggttccgt taaaccaaca gtaccaatcg gcggatggct gaagaccacg
     4141 gtcggaatgt tgctgtaatc cagatgctca tccggcttgt tattaaacag gcgttcagag
     4201 agacggcgac ccgctgcaac tgctaccggt gtcagttcca ctgcacccgt gttatcgccc
     4261 accgcgtaaa taccttcaat attggtgttt tgatatttat cgacgacgat atagcctttt
     4321 tcgttagttt taacgccagc ggcttctagg ttgatgttgt cattggcagg ctcgcgacca
     4381 atcgcccaaa tcaggcaatc caccgtttca ctgcgaccat cttccagctc cagcgtcagg
     4441 ctaccatcgg catttttcac taccgctttc gggatggcgt tggtgtgcag ctgcgggcct
     4501 tcggcgttca tcacttcgac cagcgtttcg gaaatcatcg ggtcgaagct gcgcagcggc
     4561 gcatgtttac gcacaaacag atgcgttttc gcgccgaggc cgttaatcac gcccgccagc
     4621 tcaacggcga tgtaacccgc gccaacaacc gccacgcgct ctggcaaagc aggaagggcg
     4681 aagaagccat cagaatcaat accgtattcc acgcccggaa tatccgggtg gctcggacga
     4741 ccgcctgtgg cgatcagaat atgatcggcc gtgatggttt cgccgtttac ctccagcgtt
     4801 ttggcatcaa cgaagcgggc aaagcctttg attacatcaa cgttattttt accgagcacg
     4861 ttttcatagg aagtatgaat acggtcgata taggcggtac ggctggcgat caacgtttcc
     4921 cagttgaact tgttgatggt ggtatcgaaa ccataatcag ggccgtacat atggatcgct
     4981 tcacggattt gcgccgcgtg ccacatcact tttttcggca cgcagccgac gttcacgcag
     5041 gtgccgccca gctctttggc ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg
     5101 ttgatggagg cgataccgcc gctgccgccg ccgatggcga tgtaatcata gtgtttagtc
     5161 atgacagagt gtccttatcg ttgattaccg cgattgtagc gcgagacgta ataggtgcca
     5221 gcaatggctg caattactcc ggcacgatcc agcttacggt ggcgtgcccg gtgcctgccg
     5281 gaaccagttt gctgtgcagc cacggcagca cgttattcat ctgctgttcc agtttccacg
     5341 gcgggttaat cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg tctggcagta
     5401 ccgccagttc aatttgcaga attttgcgaa taccggtcgc ttccagatcg tggatcatgc
     5461 gcttaatttg ctgacgcagc accaccggat accacagtgc gtaagtacca gtggcgaaac
     5521 gtttgtaacc ttctgctatc ccgctgacca ccgcctgata gtcggttttc atttcatacg
     5581 gcgggtcgat aaggattaaa ccacggcggg aaaccggcgg aagtttggcc ttaagctgct
     5641 ggaaaccgtc ggctttttcg acgcgcgcac ggctgtcttt ctgaaattca gaacgcagca
     5701 acgggtaatc gctcgggtgc agttcggtca gttgcaggct gtcttgttcg cgcagtagct
     5761 ggcgagcaat caacggcgac ccggggtagt aacgcaactg accgctacgg ttgaagtgtt
     5821 ttaccacatt gatgtacgcc tccagttctg cgggcaaatc gtcctgctgc cagatacggg
     5881 cgataccttc gagatattcg ccggtacgct cggcatgttc gctgcctaac tgataacgcc
     5941 cggcccctgc gtgggtatcg agatagagaa acggtttatc tttctctttc agcgactcga
     6001 tgatcaggct ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt
     6061 ggcgataact gagcatgggt aaaggtgttc cggtaagtaa aaatcggccc gtattcgggg
     6121 cgcacaaaag cgtatcagga cagtataccg aaaagaggcc gccgccgcga aagcgtaacg
     6181 tttctcattg aaattcacta cacttaaccc catgctacac acattatgta aagcgcctgt
     6241 tgagcgcttc cttaacctct ttaaccagga ctgcgcgaat gacgaatccg ttactgactc
     6301 cctttgaatt gcctccgttt tctaaaattc tcccggaaca tgtcgttcca gccgtgactc
     6361 aggcgctgaa cgattgccgc gaaaatgtgg cgcgcgtagt agcgcaaggg gcaccgtaca
     6421 cctgggaaaa tctctgccag ccgttggcgg aagtggacga tgtgttgggg cgtatcttct
     6481 ccccggtcag ccacctgaac tcggtgaaaa atagcccgga actgcgtgaa gcctacgagc
     6541 aaaccctgcc gctgctgtcg gaatacagca cctgggtagg gcaacatgaa gggctgtata
     6601 aagcgtaccg cgacctgcgc gatggcgatc attacgccac gctgaacacg gcgcagaaga
     6661 aagcggttga taacgccctg cgtgattttg aactctctgg cataggtctg ccgaaagaga
     6721 aacagcagcg ttacggcgaa attgctaccc gtctttctga actgggcaac cagtacagca
     6781 acaacgtcct cgatgcgacg atgggctgga ccaaactcgt taccgacgaa gcggggctgg
     6841 cggggatgcc agaaagcgcg ctggctgcgg caaaagccca ggccgaagcg aaagagctgg
     6901 aaggctacct gctgacgctg gatatcccaa gctacctgcc ggtaatgacc tactgcgaca
     6961 accaggctct gcgcgaagag atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc
     7021 cgaacgccgg taagtgggat aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg
     7081 aactggcgca actgctgggc tttgaaaact acgcctttaa atcccttgcc actaaaatgg
     7141 cagaaaaccc gcagcaggtg ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc
     7201 aaggcgaaaa agagctggag caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg
     7261 agttgcagcc gtgggatatc gcttactaca gcgaaaaaca gaaacagcac ctctacagca
     7321 tcagcgacga gcagctgcgt ccgtacttcc cggaaaacaa atcggttaac ggcctgtttg
     7381 aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg taaagaggtt gatgtctggc
     7441 atccggatgt acgtttcttc gaactgtatg acgaaaataa cgaactgcgc ggcagcttct
     7501 acctcgatct gtatgcccgt gaaaacaagc gcggcggggc gtggatggat gactgcgtag
     7561 gccagatgcg taaagctgat ggttctctgc aaaaaccggt cgcgtatttg acttgtaact
     7621 tcaaccgccc ggtaaatggt aaaccggcgc tgttcactca cgacgaagtg atcaccctgt
     7681 tccacgagtt cggtcacggc ctgcaccata tgctgacccg catcgaaacc gctggtgttt
     7741 ccggtatcag cggtgtgccg tgggatgcgg tcgaactgcc gagtcagttt atggaaaact
     7801 ggtgctggga gccggaggcg ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc
     7861 tgccgaaaga gttgctggat aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta
     7921 ttctgcgtca gctggagttc ggcctgtttg atttccgcct tcatgccgag ttccgcccgg
     7981 atcagggggc aaaaatcctc gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc
     8041 catctccgtc ctggggccgt ttcccgcacg ctttcagcca tattttcgcc ggtggttatg
     8101 cggcaggtta ctacagctac ctgtgggctg acgtactggc ggcagatgct ttctcgcgct
     8161 ttgaggaaga gggcattttc aaccgtgaaa ccgggcagtc gttcctcgac aacattctga
     8221 gccgtggcgg ttcagaagag ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc
     8281 agctggatgc gatgctggag cattacggca ttaagggctg atcattcagt gaaaatctgc
     8341 ttaattgatg aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg
     8401 ctggagcacg atgaagacaa cctgatggcg ctggtgttaa cgccggaaca tctggaattg
     8461 cgcaagcgtg atgagccaaa acttggcggc atctttgttg attttgttgg cggagcgatg
     8521 gcgcaccgac gcaaattcgg cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt
     8581 aaaggcgatt atttgccgga tgtggtggat gccactgcag gactggggcg cgatgccttt
     8641 gtgctggctt cagtcggttg ccgcgtgcgg atgctggagc gtaatccagt tgttgccgcg
     8701 ctgctcgacg acggcctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag
     8761 gagcgtttgc agttaattca cgcctccagc ctgacggcgc taaccgatat taccccacgc
     8821 ccgcaggtgg tctatctcga cccgatgttc ccgcataagc agaaaagcgc gctggtgaaa
     8881 aaagagatgc gtgtctttca gtcgctggtg gggccggatc ttgatgccga tggattgctg
     8941 gagcctgcac gcttgctggc gaccaaacgc gtggtagtaa agcgcccgga ctacgcgcca
     9001 ccgctagcga atgtcgccac gccaaacgcg gttgtgacta aagggcatcg ctttgatatc
     9061 tatgcaggta cgcccgtgta aaagtaaagc agggtagcga taacgctacc ctgctttctg
     9121 caagaataat taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa
     9181 cagcatcact actgcaacgc ccagcgtaac caggccaatc ttaccaaaca cgttggtata
     9241 gacgggtaac gtctcaagcg gatcggtaat gttgtccggc actgcggtaa atgttgccac
     9301 atagccgccc agcaagaatg cggcagcctg cgtcaggaac cacatcccga gaataaagcc
     9361 catcaaatgc tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacaa
     9421 ttcacccagg ctctggaata aatacaccag cacgataaac catggcgatg tcagcccttg
     9481 tgcatccgca aaccacattc ccgcagctgc cgccgtcaaa aagcccagtg agcacataaa
     9541 catgccgaga gtaaatttca tcggcatcga gaggtctttg cctttgttac ccagatgcgt
     9601 gtaaatgcct gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa
     9661 gctgaccggg tttatggaaa agccgagaat ttcatgatgc acgttgttga tggcaaagaa
     9721 gttcagcgac gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag
     9781 gacaaaggcg acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa
     9841 gaagatgatg gtgacgacga tggagagaac aatcagcacc agattggcga cttctacgtt
     9901 gtgcatcagc catgcacata cgaagatcat caccacgctg ccaagtaata cgtacaacag
     9961 tttgctgaag ctcatcggct tgaagtcggg ttcagaacca atgtctttca ccattccacg
    10021 acaggcgatg taaaccagta atgcgataat taaccccgcc ccgcacaggt tgtaggtgac
    10081 tgaataaccg tatctatcag cgatcacagg ggccagcgat aacgctatca acgagccgat
    10141 attgatcgac atatagaaca gggtgaatgc gccatcaagc cgcggatctt tcggcggata
    10201 gcacttcgaa agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt
    10261 ccccagggcg atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc
    10321 aatcgccagc acaagtgctc cgagaacaat ggtgcgtttg gtccccagca ggtggtcgcc
    10381 gacatagccg ccaatggaaa tgaggccata gaccagcgca gcaaaagcac caaaagtgac
    10441 aaaagcctgc tcttgcgaga atccaagctg tttaacgaag aaaaccgcca gtacgccctg
    10501 cacgccgtag tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg
    10561 aggttgctgc agcatcccca tgggtgttgt tgtattcata tttattaacc ttccaatacc
    10621 atcctgaaaa gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga
    10681 ttcttaatat tgatgcggtg aatagattaa tacactatta caacagaaaa taaccagctg
    10741 atcacactaa tgatggaaaa tatgatagtg ttaaatgttg tattggcagg agattttgat
    10801 tgcgtacgca gatcgtaggc cagataaggg gtttacgctg catcaggtaa caaggcgcgc
    10861 caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca cgtcgtgggg ggatgattat
    10921 tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt gatcagctga
    10981 cgtgcggaag acattagttt gctccagaag tcctggtggt gaccacaaac caccagatcc
    11041 atatcgtatt tcttgattgc atcgaccaga acctggccca ggtcgccgct gccgctcagg
    11101 gtttcagtga ttgggtagcc tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct
    11161 tcagagatgc gtttctgcat atcacccaga ttcacatcaa taagcccggt gtataggtca
    11221 gagtagttta catctacgtg gatcagagaa actttcgcat tgtaggggcg agccatagag
    11281 actgctttct ctaccagaac tttgctttcc ggggagagat cgaccgcgat gagaatgtgt
    11341 ttataagcca tagtgttact ccttccataa agttgtcgat gactggccag ctagcgtttc
    11401 ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg cggggctaac ccagccgtaa
    11461 agattcagtg ttaagaccat ccttacctta tagcgacccg gatgatccgt caatccgcct
    11521 tgcttacccg ttagataaac aaattttcga catgaatata ttgatagtgg ttaaccttct
    11581 ggaaaaaaaa caacctgatc tcctacacta tctatagagc cgctcggatg ttggtcacca
    11641 caaaagcggt ttctccagga cctggtgtta actgattggc agcgtatcgg gagcggtagc
    11701 cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg tatgataagc accgtcgcat
    11761 tattttgggc tttatgtgtc gtttgcattg ttaacatggc gcgctatttc tcatcactac
    11821 gcgcgttgtt agtggtactg cgtaactgcg atccattgct ctatcaatat gttgatggag
    11881 ggggcttttt tacctcacat ggccaaccca acaaacaggt gcgtctcgtt tggtatatct
    11941 atgcccaacg ttatcgcgat catcacgatg atgaatttat tcgccgctgt gagcgggtgc
    12001 gtcggcagtt tattctgacc agcgcattgt gtggtctggt ggtggtcagc ctgattgcat
    12061 tgatgatttg gcattaagca cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa
    12121 ctgacccgct cgagactgat tacaaacgca aaattgcctg atgcgctacg cttatcaggc
    12181 ctacataatc tctgcaatct attgaatttg catgtttttg taggccggat aaggcgttca
    12241 cgccgcatcc ggcatgaaca aagcgcacta tgtcacaatc tgaagcgggc cagctgaccc
    12301 gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga gccagtacag cccgccggaa
    12361 agcagtaccg cagccggaag ggtaaacacc caggccatca gaatactggt tacggtttta
    12421 cgctgtaagc cgccgccatc taccaccatc gtccccgcga cagaagagga gagtacgtga
    12481 gtagtggaaa ccggcatccc ggtataactc gccaggccga tagacactgc cgccgtcatc
    12541 tgggcagaca tcccctgagc gtaggtcatg cctttcttac cgattttctc accgatagtc
    12601 gttgccacac ggcgccagcc aatcatcgta ccgataccta acgccagcgc gaccgccatg
    12661 atgatccaca ccggtgcata ctcgatggtg ctaagcatgt cggacttcag tttcttcaac
    12721 aggcgctgat cgtcagcact cacgccaggc atcttcacca ctttgtcgat agtgtcagaa
    12781 acgcacagca taatgcggcg catctggcta cgttgatcaa gcgacagctt gtcgtagctt
    12841 tccacatcgg tggtcaacat acctttcagg cggttgagcg cgttaatggt attcgacgga
    12901 tggcagtgga actccgcagg ttgcgttgcg ccagcttccg gagccggtac taactgatca
    12961 gcaccggtag cctgtttgag cagcgcagga tgctgctcaa agtaagcttc gacgttgttg
    13021 atggcatcac gggtacgggt gatttcgtag ccagtggcat tcatgttcac cacgaagcct
    13081 gctggcgcga cgccaatcaa taccaacata accagaccaa tgcctttctg accatcgttc
    13141 gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca gcgcgatacg tgtccagaac
    13201 ggcggctttt tcttgccgtc tttcttttca cgctccgctg gggtcaggtg gatacgggcg
    13261 cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc
    13321 aggccgacaa taggggaaac gatcagagaa ccgaaaatac ttaatacttt cgggatattg
    13381 agtgcatcca ccactgacgt cccggtcatc aacgcattgg ttaaaccaat cccgatgatc
    13441 gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt acccaggttc
    13501 cagataatcg ccgccagcaa catagagaac accatggcaa ggccatgaga cgatcccata
    13561 ttaagcagca gatccgtcgg cagcatatgc acaatggcat aggcaacact cagaccaccc
    13621 agcaaaacac ccaaaaagtt gaacaccgcc gccataacca cggcgagctg agaacgcatc
    13681 gcgcgggtat agataacggt tgccacggcg ttggctgtgt catggaaacc attgatggct
    13741 tcgtagaaca gcacaaaagc cagtgcaagc aataataaca gcccggtatg caaatccagg
    13801 ccagcaaaca aatgtagcat aggacgttac gccattttga ggacatgaac gcggcgcatt
    13861 atcagtgact ttcgcggcgc gggcaaagtg aaatatagac tttttttgat ttgcctcctg
    13921 tatggatttc actcaaaaaa taattatctt atataattca ggcaaatact tccttttagt
    13981 aatattgatg ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttctaag
    14041 aggagcgcaa cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcgggtatgt
    14101 tctgttctgc gctggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa
    14161 aaccagggcg caaaatcctt atgtctggcg gtgggcgctg caactttacc aacctttatg
    14221 tcgaaccagg cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcacgtt
    14281 ttacccagtg ggatttcatt gatctggtca ataaacacgg catcgcatgg cacgagaaaa
    14341 cgttagggca actcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg
    14401 agtgcgagaa gggcaatgtg accttcagat tgcgtagcga agtgctgagt gtggcggagg
    14461 atgaaacagg cttcacgctt gatctgaacg gcatgactgt cggttgcgaa aagctggtca
    14521 tggcgaccgg tgggctgtca atgccggggc tgggcgcgtc gccgtttggt tataagattg
    14581 ccgaacaatt tggcctcaac gtgctgccga cccgtgcggg tctggtgcca ttcactctgc
    14641 ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta
    14701 ccgctgaaaa cggcatcgtt ttccgtgaga acttactctt cacccatcgc ggcttgtctg
    14761 gaccggcggt gttgcagatt tccagctact ggcaaccggg tgaatttgtc agtatcaatc
    14821 tgctaccgga tgtggatctc gaaaccttcc tgaatgagca gcgtaacgca catccgaatc
    14881 agagcctgaa aaacacactg gcggttcatc taccgaagcg gttggttgaa cgcttacagc
    14941 aactcgggca aatcccgaat gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac
    15001 tgattagcac attgaccgac tggcgtgtac aacccaacgg cactgaaggc tatcgcactg
    15061 ccgaagtgac gctcggcggc gtggacacca acgaactctc ttcacggacg atggaagcgc
    15121 gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga cgttaccggc tggctggggg
    15181 gctataactt ccagtgggcg tggtcgagtg cgtgggcttg tgcgcaggat ttgattgcag
    15241 cgaagtcgtc ctgacctgtt acctaaagat gaaaattatt agagccgcat taaaaatgag
    15301 ctaattttga tagtggctat cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct
    15361 ccaggcttaa tataaggtgg aaggaaaggt gattgaaaac tcacccagtg gccagatctt
    15421 tataaaatat gaacaggctg aaaaagacag taactttgtt gtttttttat ccttaaccac
    15481 tatgcattaa tgctgcgtta tttcatgatg cctaagaaaa accagcgtta cgcaaatggt
    15541 caacgctggt tttatccggt acgttgcaat tattttttag cagaacccgc ttctaatatt
    15601 gaaacgattg agaacaacgt aaagcatacc gctcccaaac cgaccagaac tcgcgaagga
    15661 ataaaatatc ccatgtcggt ctgcgccatt tccgcaagaa atgatgccag gaaaaggcaa
    15721 aacaggcagg tgaagacggg tatcatcggt attctgttgg ctaacgaaca ggtacgtctc
    15781 cagaccagtg ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa
    15841 ataagaataa tgccgggagc cagtcgggcg cttgcatcgg aactgactaa gacgtatatc
    15901 ccctgcagta ccgtgattga gccgagaaaa ataacccaat aacaccacag ccagtgttct
    15961 ttagttgaaa acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag
    16021 gcacagattg cggttaaccc aagcaatacg tgacccgcga cataatgcgg agttatgtca
    16081 gcactacgta acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg
    16141 gcaatgaggc aattaccgat taatgaagaa taagcctgta ccggcgttcc atcgctcttg
    16201 ctccccgctg catttttggg aatgagcaga aagtgaccgg atgacgctgc caccgtcgat
    16261 acacaggcgg caatcatacc aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc
    16321 atcacatcat tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca
    16381 tagccaataa tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa
    16441 atgataataa atgcggtagt gaataatgcc aggcatatgg ccgccagaga aatcagcaca
    16501 tgacccgcga cgaaatactc gctttgccct gaactttcca gcacaaagcc acccgatata
    16561 tatgttcact ttaaactcct caggtatccc tgctaataat gaaatgtgca ttaatgataa
    16621 tttcagcctg ttaatataac gacgagatat cattaaagac aaatgcatag cataactata
    16681 gcggggttta ctgtgccgag tgccattttt taccggttaa aaggccatga taaataaata
    16741 tttatcctaa gattaattct tattctgttg ttttttttac aattatcttc cagggatgga
    16801 ttgttttcca ttatttagta aaagaccgga tagtctcggt gcttaaaatc atcgaacata
    16861 taagtattat tgctttgtat catcgtaaat atgtctttca gacatgtatt gtttattttg
    16921 ttactaacaa gtaaaaatat tacaggttat ttatgaaagt atttcacaat atatttaagc
    16981 acttctcttc aaaccatcaa gataagcatt cagataaagt taacagtcat caacaccatg
    17041 gcaaggttga taaaaaatat cgatcaaaca cagttgaagt taatatgctg aatgactttt
    17101 cgcaaataga taacgattta ggcctgaata tcatctattt cctgcaacat ggtcactgga
    17161 gggtaaatga acatagtctt cagatggaga aaatatggtt ttacaatagt gagccatcta
    17221 tagatattca agaatataat agtttcgccg ataatactac taacacgttt atctttacaa
    17281 ttataccaga caataaccat gtgataaaat tatcgtctcc cattacagtt aagattgagt
    17341 gtaaggatgg ttattatttt attaatttct ccggagataa atcagacata atttataaat
    17401 cagacataac ttataaagtt aatgggcttt ctatcatacc tgaaaatttc tttactcttc
    17461 ttagcggtaa ttttaaagct gactggcgtt gggatgtctc taaagagacg tttcctaagg
    17521 ataagtttga tagctatgtt aaatctgtgt tttctaaaat agacttttat aaacaatgtg
    17581 gtgtgattaa cccacaaaat gccaacacgg cttattttgg cgatacagat ggaagagtag
    17641 gcgctgtact ttatgcattg ctcgtttcag ggcatatagg catcagggaa aagggttgga
    17701 gtttattatg tgaattatta aaacacgaaa acatggcttc atctgcatac atacataaaa
    17761 ataataaatt gctctacaat ttattaaata cgagagacat gatattgaat gagttgcatc
    17821 aacatgtttt tttaaaggac gatgcgataa ccccttgtat cttccttggg gatcatacag
    17881 gcgatagatt tagtactatt tttggtgata aatatatcct tactttgcta aattctatgc
    17941 gaaatatgga tggtaataaa gatagccgta ttaataaaaa tgtcatcgtt ctggctggaa
    18001 atcatgaaat caattttaat ggtaactata ctgcacgact agaaaattat aaactctctg
    18061 ccggtgatac gtataactta attaaaacgc tcgatgtttg tcattatgac tcagaaacga
    18121 aagttttaac cagtcatcac ggaataataa gaaatgaaga aaatgaatgt tattgtctcg
    18181 gtgctttgca ggttccattt aatcagatga aagacccaat tgatcctgag gaattagcaa
    18241 atatatttaa taaaaaacat aaacaacaca tggatgatcg tcttatccat ctaattagaa
    18301 gcaatgctgt agcatccaca cctgtatatg aaaattattt taataacact actgctttca
    18361 gaccaaaacc tgaagatatc ttcaaatgtg gacagacgct caaaaaaact aaacagaaat
    18421 atggccatta tggattaggg gtagatcagc atcaaaaaat tgataattat accatggggc
    18481 taaattcctg gaagatagca cctaacgaac gtggtgataa aaaaggtgtc ccagggctta
    18541 gttgttttca acctcaataa aacttcatga gtgctgtggt atctcttttt aattttgcca
    18601 gtatttcata ggttttctta atcgattgta gaggcatgtt ctgatgacgc ctctacaatc
    18661 tttagggact ttctctgcta tgagataaga aatttcatat tatgctttaa ggctgaatga
    18721 tttgtgcaaa atataattat tttcccatcg aacatgaggt gtatgtcata agattaaatg
    18781 ttaactatgt attcctcgcg gtaaataata tgaggtaaaa gatgaaaatt ggaactgtgg
    18841 caggcactaa cgacagtaca acgacaatca caacaaatga tatggtgcaa gagcatgtta
    18901 ccaacttcac taaagaactg tttgggtata ttgcaaatgg tataggtgat gatatatcaa
    18961 gtattgccag gacaatgctc ggtgaggttg tggaaaaaat agatgattgg caaatagaac
    19021 ggttccaaca atctattcaa gatgacaaaa tttcatttac catacaaacg aatcattcag
    19081 aaaaatatag tatgctatcg ggcatgcgcg ctcatattct acgtaggaat aactgttatc
    19141 agtttattgt gacaattaat agtaaaaatt acggttgccc tcttgataat accgatatta
    19201 attggtgcag cattgtttat ttacttaata atatgacagt aaatgataat gctaatgatg
    19261 ttgcagttac tgagtcgtac aaacccatct ggaactggaa aatatcgcaa tacaatgtat
    19321 ttgatattaa attcgaaaca atgataaaac cccagttcgc tgacagaacc tatttctcca
    19381 attgttcacc tgttgatccc acaagtacca gaccgaccta ttttggcgat accgatggct
    19441 cagtcggtgc agttttgtac gcgctttttg caacgggaca tttgggaatc atggctgaag
    19501 gagaaaattt ccttagccag ttgcttaata tagaagatga agttctcaac gtattgctaa
    19561 gggagaactt taatgagcaa cttgacacta atgttaatat aataatcagt atcctgaaca
    19621 gaagagacat tgttttagaa agtcttcagc cttatttggt tattaataaa gacgcagtca
    19681 cgccatgtac gtttctgggc gaccagactg gcgatcgatt tagtaatatc tgtggcgata
    19741 aatttatcat cgatttatta aaacgcataa tgagtatcaa tgaaaacgtt catgtattag
    19801 ctggaaatca tgaaacgaat tgcaatggta actatatgca gaacttcacg cgcatgaaac
    19861 cacttgatga ggatacctac tctgggataa aagattatcc tgtatgtttc tatgacccca
    19921 aatacaaaat aatggcaaat catcatggga taacttttga tgatcagcga aaacgttaca
    19981 ttataggacc gataacagtc tctagtgatg aaatgaccaa tgctctcgat ccggtcgaat
    20041 tagcggcaat tatcaataaa aagcaccatg ctgtaattaa tggcaaaaag tttaaaaata
    20101 gccgggctat ctcttgtcga tcctttaatc gttatttttc tgtttcgaca gattacagac
    20161 ctaaacttga agcactatta gcatgctcac aaatgttagg tataaatcaa gttgttgcac
    20221 ataacggtaa tggtggaaga gaatgtatag gtgaaacggg gacagtttta ggtcttaacg
    20281 cccgtgacag taaacatgct ggaagaatgt tcagcatgca taattgccaa attaaccctg
    20341 gtgcagggcc tgagataacc actccctgga agtcttacca gcatgaaaaa aacagaaatg
    20401 gacttatgcc tttgatcaga aggcgcacaa tgttacagct ctgaaccata gacaacatga
    20461 atattaatgt tactatctgg ttgagactgt tgataaaacg taaaaaggaa tgctctgttc
    20521 ctgcaactga acaggcgtga aacgctatcc aacaggatgg ataccgtttt gtcagccagt
    20581 tttatagatc acacttatga gtatgcgtgt ttcatttacg gccaggaatg ctgatgatga
    20641 caacattact acccgttttc actaagcctt ccccgttggc gctcaatgct ctgcgcgctg
    20701 gtcgtatttg tcgtttcctt cttatcccgg atggaagaat ccgctgaacg tcgttcggcg
    20761 gcagttgtaa cacgcgtcgt ttgcttgcag ggattatggc gggtcgatta tggataagag
    20821 taagcgccat ctggcgtggt gggttgtcgg gttactggtg gtggctgccg tcgtggcgtg
    20881 gtggctgttg cgcccggcgg gtgtgccgga aggctttgct gtcagcaatg ggcgcattga
    20941 agcgacggaa gtggatatcg ccagcaaaat tgccgggcgt atcgacacca ttctggtgaa
    21001 agaaggccag tttgttcgcg aaggcgaagt gctggcgaag atggatactc gcgtgttgca
    21061 ggagcagcga ctggaagcca tcgcgcaaat caaagaagca caaagtgccg ttgctgccgc
    21121 gcaggctttg ctggagcaac ggcaaagcga aactcgtgcc gcgcagtcgc tggttaatca
    21181 acgccagtcc gaactggact ccgtggctaa acgtcatacg cgttcccgct cgctcgccca
    21241 acgaggggct atttctgcgc aacagctgga tgacgatcgc gccgccgctg agagcgcccg
    21301 agctgcgctg gaatcggcaa aagctcaggt atcggcttct aaagcggcta tagaagcggc
    21361 ccgtaccaat atcattcagg cccaaactcg cgtcgaagcc gcgcaagcca ctgaacggcg
    21421 tattgccgca gatatcgatg acagtgaact gaaagccccg cgtgacggac gcgtgcagta
    21481 tcgggttgct gagccagggg aagtgctggc ggcaggcggt cgggtgctga atatggtcga
    21541 tctcagcgac gtctatatga ctttcttcct gccaaccgaa caggcgggca cgctgaaact
    21601 tggcggtgaa gcccggctga tcctcgatgc cgcgccagat ctgcgtattc cggcgaccat
    21661 cagttttgtc gccagtgtcg cccagttcac gccaaaaacc gtcgaaacca gcgatgaacg
    21721 gctgaaactg atgttccgcg tcaaagcgcg tatcccaccg gaattactcc agcagcatct
    21781 ggaatatgtc aaaaccggtt taccgggcgt agcgtgggtg cgggtgaatg aagaactttc
    21841 gtggcctgac gacctcgtgg tgaggttgcc gcaatgacgc atctggaact ggttcccgtc
    21901 ccgcctgtcg cgcaactggc gggcgtgagc cagcattatg gaaaaaacgt tgcgctgaac
    21961 aatattactc tcgatattcc ggcccgctgt atggtcgggc tgattggtcc ggatggcgtc
    22021 gggaagtcga gcttattgtc gttgatttcc ggtgcccgcg tcattgagca gggcaatgtg
    22081 atggtgctgg gcggcgatat gcgcgacccg aggcatcgcc gcgacgtctg cccgcgcatc
    22141 gcctggatgc cgcaggggct gggcaaaaac ctctaccaca ccttgtcggt gtatgaaaac
    22201 gtcgattttt tcgctcgcct gttcggtcac gacaaagcgg agcgggaagt gcgaattaat
    22261 gagctgctga ccagcacggg gttagcaccg tttcgcgatc gtccggcagg gaaactctcc
    22321 ggcgggatga agcaaaaact tgggctgtgc tgcgcgttaa tccacgaccc ggaactgttg
    22381 atccttgatg agccaacaac gggggttgac ccgctctccc gctcccagtt ctgggatctg
    22441 atcgacagta ttcgccagcg gcagagcaat atgagcgtgc tggtcgccac cgcctatatg
    22501 gaagaggccg aacgcttcga ctggctggta gcgatgaatg ccggagaagt gctggcaact
    22561 ggcagcgccg aagagctacg gcagcaaacg caaagcgcta cgctggaaga agcatttata
    22621 aatctgttac cgcaagcgca acgccaggcg catcaggcgg tagtgatccc accgtatcaa
    22681 cctgaaaacg cagagattgc catcgaagcg cgcgatctga ccatgcgttt tggttccttc
    22741 gttgccgttg atcacgttaa tttccgcatt ccacgcgggg agatttttgg ttttcttggt
    22801 tcgaacggct gcggtaaatc caccaccatg aaaatgctca ccggactgct gcccgccagc
    22861 gaaggtgagg cgtggctgtt cgggcaaccg gttgatccaa aagatatcga tacccgccgt
    22921 cgggtgggct atatgtcgca ggcgttttcg ctctataacg aactcaccgt gcggcaaaac
    22981 cttgagttac atgcccgttt gtttcacatc ccggaagcgg aaattcccgc aagagtggct
    23041 gaaatgagcg agcgttttaa gctcaacgac gttgaagatg ttctgccgga gtcattgccg
    23101 ctcggcattc gccagcggct ttcgctggcg gtggcggtga ttcatcgccc ggagatgtta
    23161 atcctcgatg agcctacttc tggtgtcgat ccggtggcga gggatatgtt ctggcagttg
    23221 atggtcgatc tctcgcgcca ggacaaagtg actatcttca tctctaccca ctttatgaac
    23281 gaagcggaac gttgcgaccg catctcactg atgcacgccg gaaaagtgct tgccagcggt
    23341 acaccgcagg aactggttga gaaacgcgga gccgccagtc tggaagaggc atttatcgcc
    23401 tatttgcagg aagcggcagg gcagagcaac gaagccgaag cgccgcccgt ggtacacgac
    23461 accacccacg cgccgcgtca gggatttagc ctgcgccgtc tgtttagcta cagccgccgc
    23521 gaagcgctgg aactgcgacg cgatccagta cgttcgacgc tggcgctgat gggaacggtg
    23581 atcctgatgc tgataatggg ttacggcatc agtatggatg tggaaaacct gcgctttgcg
    23641 gtgctcgacc gcgaccagac cgtcagtagc caggcgtgga cactcaacct ctccggttcc
    23701 cgttacttta tcgaacagcc gccgctcact agttatgacg agcttgatcg tcggatgcgt
    23761 gcgggcgata tcacggtggc gattgagatc ccgcccaatt tcgggcgcga tatcgcgcgt
    23821 ggtacgcctg tggaactcgg cgtctggatc gacggagcga tgcccagtcg tgccgaaacg
    23881 gtaaaaggtt acgtgcaggc aatgcaccag agttggcttc aggatgtggc gagccgacaa
    23941 tcgacacccg ccagccaaag cgggctgatg aacattgaga cgcgctatcg ctataacccg
    24001 gacgtaaaaa gcctgccagc gattgttccg gcggtgatcc cgcttctgct gatgatgatc
    24061 ccgtcaatgc taagcgccct tagcgtggtg cgggaaaaag agctagggtc gattatcaac
    24121 ctttacgtga cccccaccac ccgtagcgaa tttttgcttg gcaaacagct gccgtacatc
    24181 gcgctgggga tgctgaactt tttcctgctc tgcggcctgt cggtgtttgt gtttggcgta
    24241 ccgcataaag gcagtttcct gacgctcacc ctggcggcgc tgctgtatat catcattgcc
    24301 accggaatgg ggctgctgat ctccaccttt atgaaaagcc agattgccgc cattttcgga
    24361 acggcgatta tcacgttgat cccggcgaca cagttttccg ggatgatcga tccggtagct
    24421 tcgctggaag ggcctggacg ttggatcggc gaggtttacc cgaccagtca ttttctgact
    24481 atcgcccgcg gaacgttctc gaaagcgctg gatctgactg atttgtggca actttttatc
    24541 ccgttgctga tagccatccc gctggtgatg ggcttaagca tcctgctgct gaaaaaacag
    24601 gagggatgat gcgccattta cgcaatattt ttaatctggg tatcaaagag ttgcgcagtc
    24661 tgctcggtga taaagcgatg ctgacgctga ttgtcttctc gtttacggtg tcggtgtatt
    24721 cgtcagcgac cgttacgcca ggatcgttga acctcgcgcc gatcgccatt gccgatatgg
    24781 atcaatcgca gttatcgaac cggatcgtta acagcttcta tcgtccgtgg tttttgccac
    24841 cggagatgat caccgccgat gagatggatg ccggactgga cgccggacgc tataccttcg
    24901 cgataaatat tccgcctaat tttcagcgtg atgtcctcgc cggacgccag ccggatattc
    24961 aggtgaacgt cgatgccacg cgcatgagcc aggcatttac cggcaatggg tatatccaga
    25021 atattatcaa cggtgaagtg aacagctttg tcgcgcgcta ccgtgataac agcgaaccgt
    25081 tggtatcgct ggaaacccgg atgcgcttta acccgaacct cgatcccgcg tggtttggcg
    25141 gggtgatggc gatcatcaac aacattacca tgctggcgat tgtattgacc ggatcggcgc
    25201 tgatccgcga gcgtgaacac ggcacggtgg aacacttact ggtgatgccg ataacgccgt
    25261 ttgagatcat gatggcgaag atctggtcga tggggctggt ggtgctggtg gtatcgggat
    25321 tatcgctggt gctgatggcg aaaggtgtac tgggcgtacc gattgaaggc tcgatcccgc
    25381 tgtttatgct gggcgtggcg ctcagtctgt ttgccaccac gtcaatcggc atttttatgg
    25441 ggacgatagc gcgttcaatg ccgcaactgg ggctgctggt gattctggtg ctgctgccgc
    25501 tgcaaatgct ttccggtgga tccacgccgc gcgaaagtat gccgcagatg gtgcaggaca
    25561 ttatgctgac catgccgacg acacactttg ttagcctcgc gcaggccatt ctctaccggg
    25621 gtgccggatt cgaaatcgtc tggccgcagt tcctgacgct gatggcaatt ggcggcgcgt
    25681 ttttcaccat tgcgctgctg cgattcagga agacgattgg gacaatggcg taaggaaggg
    25741 aaacttgact tcatactgtg atgtcatact attgatgtca taaagtgaag tcataaggac
    25801 atgccatgag aaccaaggta caggctttac ggaagaaaaa aaaatacatt ggatcaaata
    25861 tttaaaactc ctgttcctca agggatcaag tggtctgata tagagtcact ggttaaagca
    25921 ttaggcggag aaattaagga aggaagaggt tcgcgttgta agttcatact aaatatgagc
    25981 gttgcgtgtt tccatcggcc tcatccgtcg ccagataccg ataaaggcgc tgtagaaagc
    26041 gtgcgtgact ggttactaag tataggggta aaaccatgat caaactgaag acgccaaact
    26101 ctatggagat tgccgggcaa ccagctgtta ttacctacgt gccagaactg aatgcatttc
    26161 gtggcaaatt cctgggcttg tcaggttatt gtgattttgt ctctgacagc attcaggggc
    26221 tgcaaaaaga aggggaactt tccctgcgag agtatcttga ggattgtaaa gctgcgggta
    26281 ttgagccgta tgcgcgtaca gaaaagatta agacctttac gttacgctat ccagaatcgc
    26341 tgagtgaacg ccttaataat gcggctgcgc agcaacaagt ctctgttaat acctatatta
    26401 ttgaaacact gaatgagcgt ttgaatcatt tataaatttt agcggcgtac attacgcaga
    26461 atatctgcct ttacaggatc tatctatgca actgaactta attttataaa ttaagttcag
    26521 ttaatatctt ttctttcctc gtttcagaaa taactcaatc ttccttcggc aagcactgca
    26581 aatgcccatg ccgcacgccg cgctgggcga taacgtcatc ggcaaaatgc tgcacgtcgc
    26641 ccatatcacc tttcagcacg gcgatttcca gacagtcgtc gtgattgata tgcacatgca
    26701 gcgtggcgac ggagagatcg tggtgatggt gctgggtgga gacaatgcgg ctggctaagt
    26761 cgcgtttttc gtgttcatac acataggaga gcaccgcgaa accttgcgtg ccgtgctgct
    26821 gagtggcctc ctgggccagg gcgctacgca gaatatcgcg gatagcttcg gaacggttgt
    26881 tataaccacg acgctggctc aggctgtcca gcgtctccag taaatcgtca tcaagcgtga
    26941 tggtgactcg ttgcatttgc gttaaacctt ttctgtggtg cgacggcgca cggggaatgc
    27001 gggtaatacc gcgttttgta gcacacgtcc ggcgtcagag gaaaaggtta atttatctcc
    27061 caccacctgg gtttcgacga tttgtccgtt gtccatcacc atcacccgct gacagaagcg
    27121 ttccaccagg cgtaagtcgt gggtgatgaa caggcaggcg gtgccaaact gttgttgtag
    27181 ctttttcagc aggcgaatga cgcccgcctg taacacgaga tcaaggttag aaacggcttc
    27241 atccagaatc agtagtttcg gttcgaccgc cagcgcgcga gccaggcaga cgcgctggag
    27301 ctggccgccg cttaactgcg gtgggcgttt gtcgagaacg ctgtcatcga gatcgaccgc
    27361 cttcagcatt tcgctggcgc gcgccagttg ttcggatttt ttcagtgata gcaggtggcg
    27421 catcggttca cgcaggatct cgcgcacggt tttgcgcgga ttcacggcgc tgatggagtc
    27481 ctgaaatacc atctgaatat cgcggcggaa cgctttacgc tgggcgcgat tgagtttcgc
    27541 cagcggttcg ccacgccagc taatattccc ctgcgcgggc gattctaaac ccaccagcaa
    27601 ccgcgcgagg gtacttttcc cgcagccgct gcgccccagc agggcgacgg tttcgccgct
    27661 tttcagggtc agggaaacgt tattcagcac cgcctgatgt tgatgttttc cgttaaatcc
    27721 accgtgggca tagtgatggg aaaggccgga gacgttaagt aaagtcatga tgccagctcc
    27781 ataccgtaga gggcgagatg agcggaaacc aggctgcgcg ttaccgcatg tttgggggcg
    27841 ttaaacagcg tttctacatc gccctgttcg acaattttac cttgtgacat caccgccacg
    27901 tcatccgcca gacgcgccac aacgcccata tcatgggtga ccagcagcat tcccggcgct
    27961 tgtttttgca taatgctttc cagcagatcg aggatgcgcg cctgtgctac cacgtcgagg
    28021 tcggtggtcg gttcatcggc gatgataaac ggtgattcac acagcaccgc catcgcaatc
    28081 atcatgcgct gcaacatgcc gccgctcatc tcgaacgggt acagcttcag cacgcgcgcg
    28141 gcgttttcca gccccaccgc ttctatggca gcggtaagcg tagcgtcatc ggcgggtttc
    28201 cctaacgcca ggcaggtttc ccgcgcgtgg gtgtgcatgg tgtgtagcgg attaaaggcg
    28261 ctgcgcgggt tctgcatgat ggtggcaatt ttgatgccgc gcagggcgca gggataaacc
    28321 gctttgccat cggctaaaat ttcccccgcc gtctggcgaa cgccagcggg caaaatgccc
    28381 agcgtcgcgg cgcaggttaa tgatttcccg ctgccgctac cgccgactaa cgccagcacg
    28441 cgcccgcgtt gcagggttaa cgatacaccg tgtaccagcg gctgcgcggc ctgtagcgcg
    28501 atattacgta gttcaatctg ttgcggcatt agtgtgcgtg ctccgtcacc agatgaggat
    28561 ccagatgatc gcgcagtgcg tcacccacca ggttaaaggc catcacgctg ataaacagcg
    28621 ccagccccgg ccagaacatt tgcagcggct gggtccagat atactggcgc gcgtcgttaa
    28681 tcatcacgcc ccattcggcg gtcggcgcgg tcacaccgag gccaaggaaa gacatccccg
    28741 cgacgtgcag catcatatgg ccgatatcca gcgttgccag caccagcagc gaagggatca
    28801 ccgcgcctgc cagatgatcg acaaacaccc gcacatggcc cgcaccggaa agccgtgacg
    28861 ccagcacaaa ctcgcgttgg cgtagtgaaa tcaccaagct gcgcaccatg cgtgcatacc
    28921 acgcccagtg cgacagggcg atggcgataa ttacgttggt cagcccggtg ccgagcacgc
    28981 caaccataaa gaacgacaga atcgaggtcg ggaaggtcat aaacatatcg gcgacgcgca
    29041 tggtggcctg atcaacgcgc ccgccaatca acccggcgct gccgccaata accagcccta
    29101 atgtcagcac cagcagcagg caggccatta ccgaaccgag cgacacgcgg gtcgctgcca
    29161 tcagccgcga gaaaatatcg cgacctaagt gatcggtgcc cagccagtgc tgcgcatccg
    29221 gcgaaagcag gcgcgacggc aaatcaatcg cctgggggtc atacggcagc caccactggc
    29281 tggtgagcgc aatcagcgcc agcagggcga taatgatcag cgccaggcgt accgaccagc
    29341 gggaagagag gaaaaagttc acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag
    29401 cgcggcgttg agcaaatcga caatcaaatt acagaccaca aaaaccacca ccatcatcag
    29461 cgtaaagcac tgaatcaccg gatagtcacg gttaaaaatc gccgacaccg catagcgccc
    29521 gacgcccggc caggcaaaga tgttttcgat aatcatcgtc ccgccaatca gttcgccgat
    29581 gtgcatcccc acggcggtga tcatcggcag cgaggcattg cgcagaatat ggcggcgttc
    29641 ggtctgtttg tcgttcaggc cgcgcagacg cgcccaagtg acgtgacgct gaccggcgac
    29701 gtccagcata ctggcgcgca gcagtcgcgc gttaatcgcc agcgacataa aggcaatgga
    29761 aaccgcaggc aaaatgatgt gctgccagcc gccgtaaccc atcgcgggta gccattgcag
    29821 atacaccgaa aacgccatca ccagtaaaaa cgccagccag aagttaggca tcgacacgcc
    29881 aagaaacgcg atgaaacgta cggcgaaatc cggcagacgg tcgcgatggc gcgccgccca
    29941 gataccgagc ggtacggaag tgagcagaat taataccagc gccgcacctg caagttccag
    30001 cgtggcgggc aggaagttca gcatatcgtc cagcaccggg cgttggctgg cgaatgagat
    30061 accaaagtca agatgcagcg ccttccacaa ccaggtaccg tactggacgt acagcggctg
    30121 atccagcccg agcatggtgc gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga
    30181 taaacgcaga taatcgagcg ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat
    30241 caccgaggcg gcgagcacca tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa
    30301 cattaaggtt tcaccggttt aatctgttcg aacggaattt cggtggcgat cggcgcgtag
    30361 gggatattac ccagctccgg ttttgatacc accatcatcg agatgtagct gataggcaga
    30421 taaaccgcct cgtcatgcag acgggtcaaa atgtcgcgat acagcgcctg acgttgcgtt
    30481 tcgtcatggg tcgccagcac ttcgccgatc tctttatcaa tcagcggctt gtcggctaat
    30541 ccttgctgtg cctggaagtc agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt
    30601 ggatcgtatg gcgcgcccca ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga
    30661 cgagcataga tactgctctc ttcttcgcca atcagcgaga catctgcgcc aatctggcgc
    30721 atatcagcct gaatgatttc cgccatcgat ttgcttaacg catcggtgcc gatgaacgac
    30781 agttcaatgc gcagcggctg accatttttc tcgcggatgt ctttgcccgc aggcagcgtc
    30841 caaccggctt tttccagcaa ctctttcgct ttctgcggat cgtactggcg cggtttcagg
    30901 ccgagattag cgtaaggcac agaaggggca aacagggtgt cggcgacctg cggggtgcca
    30961 tataacgcgt tatcaatcag cgattttttg tttaccgcgt aattaagagc ttcacgtact
    31021 gccagctcgt tggtgggggc tttggcggta ttgagcgcca gcatcacggt ttcgatcggc
    31081 tgtgacagtt gggtgtggta agccggattc tggctaaagc gggcgaaggt atcgagcggt
    31141 aataaccctt cgtttccgta cagcaggtcg atatcgccag tttcaaacgc caccgcgcgg
    31201 gtagtcgggt ccgggatgac gttaaaggtg atctttttaa tcgctggctt ttcgccccag
    31261 tagttttcgt tacggacgaa gacatcgtac tgattcagtt tcgattcctg caaaatccac
    31321 ggtccggtgc caatcggcgc tttaattccg ttcatggttt catggttttt aaactgcgag
    31381 ggagcgataa aacggaaagg acggggcagg gccagttctt gcaggaaagg atagtaggcg
    31441 cttttcaggg taatttgcag ctctgtttta ctgagtgctt taacatcaac aatctggttt
    31501 gccagctcca gccaggcgtg acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc
    31561 gccgcctcgg catcgaacgg ttcaccgttg gagaatttca cgtcatcacg cagggtgaag
    31621 gtccaggttt taccatcttc tgaatgagtc cagctttttg ccagccacgg gatcaccgaa
    31681 ccgtctgcct gatatttcac caatggttca taaaccatgc tctgggcgaa catctggtta
    31741 ggcgtgtaaa ggtgcgggtt tagtggcccg acattcaccg gccaggcggt agtgatttca
    31801 tctggtgcag cggcatggac gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg
    31861 cggagtgtgg agagcatggt aaccccaatg gattaaaata gatggcggaa ataagtatga
    31921 cgattttaag tattcgtcat actgattacc tgttctggat caatagatgg gcagtcgggt
    31981 gggcggatca gttaactgaa tcgatccatt gcacactgtc ggcggtgagc gtaaaggggg
    32041 tgggggtgca gatcgcaagg cttaaatttt ctagctgaca atggctgact gacagcgagg
    32101 agtgataggt gctatctacg ctgacgattt gccaggcgct gccgccacgc tgtttaacga
    32161 tggcttcttt gcgcgtccag atgcgccaga acatttccag ttgctgatca ggatgcacgg
    32221 cgtccatttc agcgtgttcc ccgaggctga acacggcgtt cgccagccag cgccagttgg
    32281 cgcgcgggcg aatcacttcg atatcgcagc cgacttcgcc ttcatcgctc aacagcagag
    32341 cgatatcgtc accgctatgg cttaagttga accatagcgg cgtttccggc gcgaatgcag
    32401 gtttgccttg ttcgccatag atgatctccg gtagcgggga aagcgtgtgc gaaagcaatg
    32461 cacgccccgc cagccagcgt tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaacccg
    32521 gtggcagtgg agctgcgctt aaggtcgaaa ctttccccag aactatccga tacatatcag
    32581 ggccaacgtt taatggaaaa tgaaagtgcg tatcgtatca cttgtcgcct catcccggta
    32641 accgactttt cggtctgcct ggccccagta aaatcgccag tttgctaccg cctttggtgg
    32701 tttccatcca gattttacac acactggtta atggcaccga aagtagcatc cctaccgggc
    32761 cgagcagcca tccccaaatt aacaatgaaa gaaataccac catggtggac atccccaggc
    32821 gatggcccat catccgtggt tctaaaatat tgccgatgac catatggacc actaaaaaca
    32881 atgcgccgac cagaatacat tcgtaaacac cattaaacag cagcacctga atcattggcg
    32941 gtacggcgga aattaccgcg ccgatattgg gcacgtagtt gagcaaaaac gccagtactg
    33001 cccacatcag cgcaaactgc acccccatca gctccagccc cagccagacg atgacacctg
    33061 tccataaact gagtagcgtc ttcaatgcaa gatagtgcga aacgccttta agtgcgcggt
    33121 gtaatcctgc gatgtgaatc tgtgggttat tcagcgcaaa acgcattttg taagggacgt
    33181 ggcgcacttc aaacagcata aaaactacgg tcatcaccag caaaagcacg ctcgccattg
    33241 ccccggaaag cccggtcatt agcgctgtgg tgaaggtaac cactttttcc gagtccatcc
    33301 gctgcagcat tcgctccggc gacatatgca aattaagaaa aggcaacatc tcctgcaatt
    33361 taaaaagttt gcgcgtcagc tccttattaa acttcggcag catagagata aattcgttaa
    33421 acgatgccgc cagtacgccg accagcgcgg ttagtgcgat cagcatcacc accactacaa
    33481 tcgtaatggc aacggggcgt tgtactcccc gacgaataaa ccaggtaacg agcgggttga
    33541 ggacgatggc aaaaaacagc gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa
    33601 tgcccgcgag gatcactacc agcgaggcca gcttgagcag aatgtgcatg cccgttttat
    33661 cgggttgagg gatttccatt ggggcttcct tgtgactttt tgtattaagt gtagcgggag
    33721 tcacgccagc aatattctga ttcttactgc tgattttcac gtcagcacgt ggtaaaaatg
    33781 aaacactgtt gtaaaaatgt ggtgatcctc atgcccgaac ccgtagccga acccgcgcta
    33841 aacggattgc gcctgaattt gcgcattgtc tccattgtca tgtttaactt cgccagctac
    33901 ctcaccatcg ggttgccgct cgctgtatta ccgggctatg tccatgatgt gatgggcttt
    33961 agcgctttct gggcaggatt ggttatcagc ctgcaatatt tcgccacctt gctgagccgt
    34021 cctcatgccg gacgttacgc cgatttgctg ggccccaaaa agattgtcgt cttcggttta
    34081 tgcggctgct ttttgagcgg tctgggatat ctgacggcag gattaaccgc cagtctgccc
    34141 gtcatcagcc tgttattact ttgcctggga cgcgtgatcc ttgggattgg gcaaagtttt
    34201 gccggaacgg gatcgaccct gtggggcgtt ggcgtggttg gctcgctgca tatcgggcga
    34261 gtgatttcgt ggaacggcat tgtcaattac ggggcgatgg cgatgggtgc gccgttaggc
    34321 gtcgtgtttt atcactgggg cggcttgcag gcgttagcgt taatcattat gggcgtggcg
    34381 ctggtggcca ttttgttggc gatcccgcgt ccgacggtaa aagccagtaa aggcaaacca
    34441 ctgccgtttc gcgcggtgct tgggcgcgtc tggctgtacg gtatggcact ggcactggct
    34501 tctgccggat ttggcgtcat cgccaccttt atcacgctgt tttatgacgc taaaggttgg
    34561 gacggtgcgg ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta
    34621 ttccctaacg gcattaaccg tatcggcggc ttaaacgtgg cgatgatttg ctttagcgtt
    34681 gagataatcg gcctgctact ggttggcgtg gcgactatgc cgtggatggc gaaagtcggc
    34741 gtcttactgg cgggggcagg gttttcgctg gtgttcccgg cattgggcgt agtggcggta
    34801 aaagcggttc cgcagcaaaa tcagggggcg gcgctggcaa cttacaccgt atttatggat
    34861 ttatcgcttg gcgtgaccgg accactggct gggctggtga tgagctgggc gggcgtaccg
    34921 gtgatttatc tggcggcggc gggactggtc gcaatcgcgt tattactgac gtggcgatta
    34981 aaaaaacggc ctccggaaca cgtccctgag gccgcctcat catcttaaaa cttactgaat
    35041 aaccagcgta ttaatgatgt tttctgcggt ggtctgcgct ttttgctgat cgtcagctgg
    35101 cagagtaatt tgcatggtca gcagttgatt acccacgtta cccagaataa cggaagagta
    35161 cgccgtctgg cctttcgcgg agataatact gtctaactgc tgcattttgt gacctttcag
    35221 ctcaatggct ttgttggtta ccacttgcag ctgcggatcg cggctacgtt gctgatcttc
    35281 cagacgcttc gccaacaccg ccagatcttc tttcggatca tcgcccatga tgacgatgac
    35341 tgctttctgc ccggtggcgt cggaccagac atgcatgtta ttggcctgcg ttcccagctt
    35401 accgctctgg tcggtcatat ccgctggcag cgagaaactt aacttgccat caagcaggtt
    35461 gacgggattc ccggtagcgt tactttccgc gaccgaaccc tgcgccgtag cgttagtgtc
    35521 tttatcatca caggccgcaa gccccataac cagcaggcca attccgacat atttaaccag
    35581 attgcgcatt gacttcttcc tttcgataaa cggccataac ggctcattca tccatcttat
    35641 cacaactctg ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg atttatctgc
    35701 cagtcttttc aacagaatat tgagtaatac gccatacatt ggcaggaaga aaacgatact
    35761 gattaacact ttgaaacagt aatcgaccag cgcgatttcc atccagtgtt cagccataaa
    35821 ggcatccggg ctacgccaga aggcaatgaa gaaaaaggcc agcgtgtcgc tgacgttacc
    35881 gaacagtgtg gacgctgtcg gtgccagcca ccagcggcga ctctgacgca ggcggttaaa
    35941 aacgtgcacg tcgaggatct gtcccagcgc gtaggccatg aaactggcgg tggcgatacg
    36001 ggcgacaaac aggttgaagt gggcgagtgc gccgaatccc tgccaggaac ccatatagaa
    36061 tagcgacgag atgacgtagg agatcagcaa cgccgggatc atcaccgcaa agatgatacg
    36121 acgggccagc ggtgcgccaa aaatacgcac ggtcaggtcg gtagcaagga aaataaacgg
    36181 gaagctaaac gcgccccagg tggtatggaa acccaaaatg gagacgggaa gctgaaccag
    36241 atagttactg gaggtgatca ccagcagatg aaataacgat aaccagaaca acgccttata
    36301 gcgttgagtt tgcgagaaaa cgttcatatt gtaccttttt gattaaccat tggggtgagg
    36361 gaacccaata cgtacgacac gtctgttacc ttaacataat aacgtgctta cccttttttc
    36421 gagccgccgc atgatactgc tttacgttga caatgcaatg gttaattttc acgcaatcgt
    36481 taacctggtt tgcttacgga ctcacagggc gtaaactagc gccgtttttt tatgtgatga
    36541 gaagaaaatg accgatctct tttccagccc tgaccacaca ctcgacgcac ttggcctgcg
    36601 ctgcccggaa cccgtaatga tggtgcgcaa aactgtgcgc aatatgcagc ctggcgaaac
    36661 gttgctgatt atcgccgacg atccggccac tacccgcgat attcctgggt tttgtacctt
    36721 tatggaacac gaactggttg cgaaagagac ggatggactg ccttatcgtt atttgattcg
    36781 taaaggcggt tgatggggaa agattggctt cgatgccgcc ttttcccctc accctaaccc
    36841 tctccccaga ggggcgaggg gaccgatcgc gctcaatgtt gcgatcggtt tgccttatct
    36901 cctgcgcaac aatcttaacg cattcgctgt caccagcacc gtcgcacccg tatctgccag
    36961 caccgccagc cacaggccgg tcatccctaa cagcgtggtg acgaggaaga tccctttcag
    37021 ccccagcgca atggtgatgt tctggcggat attggcgtga gtggcgcgtg ccagttcaat
    37081 catttgcacc aggccgcgca ggtggttatg ggttaatgct gcatcagcgg tttccagcgc
    37141 cacgtctgtg ccactgccca ttgcaatccc aatggcggca gctttcatcg ctggcgcgtc
    37201 gttaatacca tcaccgacca tcgccagggg cgcatgttga ttcagcttgg tcaccgcttt
    37261 gactttatct tccggcaata ggcccgcttt aaactccagc cccagctccc cggcgattgc
    37321 cgccgctgca cgtggattat cgccggtgag gatcactcct ttgaccccca gcgcgttcag
    37381 ttcactgatg gcagttgcag catcggcgcg cagggtatcc tgcaatgcaa tgatacccag
    37441 cacgtcatca ttacgtacta ccagcacaac cgtttgcccg gcgctttcca gttcattaat
    37501 caaaccagca aatgcatcag cgggatgttt cccggcagcg caaatcaata cgcgctcacc
    37561 gttaacctgc gcttcaatgc cagacccgac cagcgcccgc tgtgattcgg cggtgggaat
    37621 ggcgagttca gcaacctgtg cttcgcgtac gatggcttgc gccagtggat gcgtcgcgcc
    37681 ttgctcgacc gccgccgcca gtgtcagcag ttcagattca ctaatacccg ttgccggatg
    37741 aatcgcggta acgcgcggtt taccgacggt cagcgtaccg gttttatcaa acgccacctg
    37801 agtaacacga cctagttgtt ccagcgccgc tccgccttta atcaacgccc cacgacgcgc
    37861 tgccgccgcc agcccggagg taatcgccgc aggcgttgag ataactaacg cacacgggca
    37921 gccaatcagc agcagcgtca gccctttata aatccactcc tgccagctgg cggcaaacag
    37981 cagcggtggc accagcgtca ccagcagagc gacggccata atcgcgggcg tatagatacg
    38041 gctgaaacgg tcgataaacc gctcaatggg ggcgcgacgc tcttcggctt cttcgatcag
    38101 tttcagaatc cggtcaatgg cgctggcacc cggttctgac agcacttcca gcgttaccag
    38161 acggtctacg ctggtggctc ctgcaggaac tttatcgccc gtcgcgcgct ccaccggaat
    38221 ggattctccg gtcagggcgc tttcatcaaa actggcaaac ggtgagagca gtttaccgtc
    38281 ggcaggcaaa cgcccacctg cggcgacttc aatcacatcg ccaggccgca ggctgttaat
    38341 cgccacctct tcccgctcac cgttacgcag gcgcgtggcg gtttctggtt tcagcgccat
    38401 taacgcgcta acgccctgac gcgcgcggct ggcggcccag ccttccagtc gttcaccaat
    38461 caaaaacagc agcaacacca tcgcagcttc agccgttgcg ccaataaaca gtgcaccaat
    38521 agcggctacg ctcattaagg tttcaatggc gaagtagctg ccggatttga tcagccgtaa
    38581 tgcctgacga gcaatcgggt acagcccaac cagcgtggtc gcgataaacg ccagttgccc
    38641 gaacggatga ttgaactgct ccagacccca gctgattgcc atcatcacga ttagtgtaat
    38701 cagcggcaga ttctctttca ggcgcgatgc ttgtggctcg tcggcggcct gctcatcgcg
    38761 cagggaatag cccgcttttt gcaccgcaga ttcaacttgt gcacgaatgt cattgtcggc
    38821 atcaaccacc agtttttcgg tggcgaacaa cacctgcacc tgattcacac ctgcaagctg
    38881 gcgcacggca ttttctacct tgcgcgcaca ggcggcgcag tccatgccac tgactttcca
    38941 gctatagcgg gtgccggaga cgttttcaga gagcgttggc gagctggagc atgcgccgtc
    39001 gcagcaacag tcgttggcgt tctgtaccgt ggttagcggt ttgaacgcag caaattgagg
    39061 ggctttcttg ccgtgattgt caggtgtcga catggcatac tccggttaag ttttttctca
    39121 ttaaccgaag gatacactct ggagtcgact ccagagtcaa gttttatcag agatacagtg
    39181 agcggatgat gaggaagtga ccggcaaagt agcaggccgc agcaatcgcg ttatccgcac
    39241 ggaagcgtcg gcgatagtgg ctccccagcc agacaaagtt actgataaac agcaacgaag
    39301 cgccaacaaa cgcagagagc gccggagcgg tcggacggaa gaaccacagt tcacctgcca
    39361 gccacaccat caccagcgtc atgccgataa acgtgcagat aggccaacga tactcttcca
    39421 gccgcgtcca gataatcgcc agtaacagcg cacccagaac cagcaacacc agtggcagag
    39481 gccagaagaa agagagcgtc atctgactgg cgaaatagat ggtgtacagc aggtgcgaga
    39541 ggaaaaacgc gccgatggcg tacatcagac gttgacgtgg caacagggtt agcgcatcgc
    39601 ccagcagtga ggcgcacagc cctgccagca ccagatagct aatggcgtcg aacatcggcg
    39661 cttgccaggc cagtaacagc aggagaagaa gggttaacgg tttaaacacc cagcgttgcc
    39721 aggttggccc acgatacgat gcatccacag atagccatgc ggaaagacag acagcgataa
    39781 acgaccaaag catcttagtt ccttgaattc gttatttttt cgtaagcgga atgcctacgg
    39841 tcttgcttcc agtttagtgg cgagggtgga cggatgacaa caccataact ggcagggtat
    39901 gcttatttcc gcattttccg atgagggata aagatgagca aaccacctct tttctttatt
    39961 gttatcattg gcttaattgt cgtcgccgca tcgtttcgtt ttatgcaaca gcgacgggaa
    40021 aaagctgata atgatatggc tccgctccag caaaagctgg tggtggtgag caacaagcgg
    40081 gaaaaaccga ttaacgatcg ccgttcgcgc cagcaggaag tgactccggc aggcaccagt
    40141 atgcgctacg aggcaagctt caaaccgcaa agcggaggaa tggagcagac gtttcgcctc
    40201 gacgcccagc agtaccacgc cctgacagtg ggcgataaag gtacgctgag ctataaagga
    40261 acgcgctttg ttagctttgt tggcgaacaa taaggttatt ttttaacttt aaatttcttc
    40321 tgccagacca gcagttcaaa cacgccgaaa aggaaaatcc gtaccttttc ggcggtggtc
    40381 atctgcgggc catctttcgg taaagtggat ttcaatagcg ccagctgcat accatgcatc
    40441 agcacggtaa aaatcagcgc cacgttaacg aaaatattta gcgggcgcgg gaagggatgc
    40501 accagattga ggattaaaaa tccccaaacg cagagcatta acaaacgacc aatattaatc
    40561 agcatcactt tctccttgtg cttcgcgttg atacagccga taagccacct gacccgccac
    40621 tttttcccga tgtaacgacc agtttgccgg aacggtgggc agaccgtttt cgacttcgct
    40681 ttcgacataa atcagggctt cgtcagccag ccagccgtta tcttccagta aatttatcgt
    40741 ctcttctaac aagccacggc ggaacggtgg atcgacaaac acgatattat gcggtgtgcc
    40801 tttttgcgcc aggaatgaca tcgcgttgct gttcaccacg cgtgcattgc ctgcttttag
    40861 tgtcgccaga ttcttaatta actgctgaga aactgcacga tccatctcaa ttaacgtggc
    40921 ccccgcagcg tagcgcgata acgcttccag ccccagcgcg ccgctcccgg cgaagcaatc
    40981 cagacattgg gcgtcaacaa tgaccggagc cagccagtta aacaacgttt cgcgtacgcg
    41041 gtcggtggtg gggcgcagac ctgggctatc aggaaccggg agtttacggc ctcgccactg
    41101 cccgccaata atgcggattt ggccgctgcc ggaatgattc ggttttttca tgataaattg
    41161 ctcaatccgc cagataacac atacttgcag gcggtgatgt gaattaagtt aagtgataga
    41221 ctatttcatc atttttttag ctgctatgta catagcgtta acgctgtgcc atgaagcaac
    41281 agcgaggagt gtagtcgcaa atggcgaaag aaaaaaaacg tggctttttt tcctggctgg
    41341 gctttggtca aaaagagcag accccggaaa aagagacaga agttcagaat gaacaaccgg
    41401 ttgtagaaga aatcgttcag gcgcaagagc ctgtgaaggc ttcggaacaa gccgttgaag
    41461 agcagccgca ggcgcatact gaagccgagg cggaaacttt tgctgccgac gttgtggaag
    41521 tcactgaaca ggttgttgaa agtgaaaaag cgcagcctga agcggaagtt gttgcacagc
    41581 cggaaccggt cgtagaagaa acgccggagc cagtggctat cgaacgtgaa gagctgccgt
    41641 tgccggaaga cgtcaacgcc gaagaggttt cgccagaaga gtggcaggct gaagcggaaa
    41701 ccgtagagat tgtcaaagcg gcggaagaag aagcggctaa agaagaaatc accgacgaag
    41761 agctggaagc acaggcgctg gcagccgaag cagcagaaga ggcggtgatg gtggttcctc
    41821 cggcagaaga agagcagccg gtggaagaaa tcgctcagga gcaggaaaaa ccaaccaaag
    41881 aaggtttctt cgcgcgcctg aaacgcagcc tgttaaaaac caaagaaaat ctcggttccg
    41941 gatttatcag cctgttccgc ggtaaaaaaa tcgacgatga tctgtttgag gagctggaag
    42001 agcagctttt gatcgccgat gtgggcgtgg aaaccacacg taaaattatc accaatctga
    42061 cggaaggcgc atcccgcaag cagcttcgtg atgccgaggc gctctatggc ctgctgaaag
    42121 aagagatggg tgagattctg gcgaaagtcg atgagccgct gaatgttgaa ggtaaaacgc
    42181 cgttcgtgat cctgatggtg ggcgtcaacg gtgtgggtaa aaccacgacg attggtaagc
    42241 tggcacgtca gtttgagcag cagggtaaat cggtgatgct ggcggcgggc gatactttcc
    42301 gtgcagcagc ggttgaacag cttcaggtct ggggtcagcg caacaatatt ccggtgattg
    42361 cccagcatac cggggcggat tccgcctctg ttatcttcga cgccattcag gcagctaaag
    42421 cgcgtaatat cgacgtcctg attgccgata ccgccggacg cctgcagaac aaatcgcacc
    42481 tgatggaaga gttgaagaaa atcgtccgcg tgatgaagaa actcgacgtt gaagcgccgc
    42541 atgaagttat gctgactatt gatgccagca ccgggcagaa cgcggtaagc caggccaaac
    42601 tgttccacga agctgttggc ttaaccggca tcacgctaac gaaactggac ggcacggcga
    42661 aaggcggggt aattttctcg gtggctgacc agtttggtat ccctatccgc tacattggtg
    42721 tcggcgaacg tattgaggat ttgcgtccgt ttaaggcgga cgactttata gaggcacttt
    42781 ttgcccgaga ggattaacaa tgattcgctt tgaacatgtc agcaaggctt atctcggtgg
    42841 gagacaggcg ctgcagggcg ttacgttcca tatgcagccg ggtgagatgg cgtttctgac
    42901 cggtcattcc ggcgcaggga aaagtaccct cctgaagctg atctgtggga ttgagcggcc
    42961 cagcgccggg aaaatctggt ttagcggcca tgacatcacg cgtctgaaaa accgtgaagt
    43021 tccgtttctg cgccgccaga ttggcatgat tttccaggat caccatctac tgatggaccg
    43081 tactgtctac gataacgtgg cgatcccgct gattatcgct ggtgccagcg gtgacgatat
    43141 tcgtcgccgg gtgtcggcgg cactggataa agtcgggcta ctggacaaag cgaagaactt
    43201 ccctattcag ctttccggcg gtgaacaaca gcgtgttggc attgcccgcg cggtggtgaa
    43261 taagcccgcg gtactgctgg cggacgaacc gaccggtaac ctggacgacg cgttgtcgga
    43321 agggattcta cgtctgtttg aagagtttaa ccgcgttggg gtaaccgtat tgatggcaac
    43381 gcacgacatc aacctgattt cacggcgttc ccatcgcatg ctcaccctga gcgatggtca
    43441 cttgcatgga ggcgtgggcc atgaataagc gcgatgcaat caatcatatt cggcagtttg
    43501 gtgggcgtct ggatcgcttc cgtaaatcgg tcggcggctc aggcgacggc ggtcgtaacg
    43561 caccgaaacg cgcgaaatcc tcgccaaaac cggtaaatcg caaaaccaac gttttcaacg
    43621 aacaggtgcg ctatgccttc cacggcgcat tgcaggatct gaaaagcaaa ccgttcgcca
    43681 cgtttttaac ggtgatggtt atcgccatct ccctgacgct gcccagcgtc tgttatatgg
    43741 tgtacaaaaa cgttaatcag gcggcgacgc agtattatcc gtcaccgcaa atcactgttt
    43801 acttgcaaaa aacgctggac gatgacgccg ctgcgggcgt ggtggcacag ttgcaggccg
    43861 agcaaggcgt ggagaaagtg aactatcttt ctcgtgaaga cgcactgggc gagttccgta
    43921 actggtctgg ttttggtggt gcgctggata tgctggaaga aaacccgctt ccggcagtgg
    43981 cggtggtgat cccgaaactc gattttcagg ggacggaatc actcaatacg ctgcgtgatc
    44041 gtatcacgca gattaacggc attgacgaag tgcggatgga tgacagctgg tttgcccgtc
    44101 tggcggcgtt gaccgggctg gtcgggcgcg tttcggcgat gatcggcgtg ttgatggtgg
    44161 cggcagtgtt cctcgtcatc ggcaacagcg tacgtctgag catctttgct cgccgtgact
    44221 ccatcaacgt gcagaaactg attggtgcga cggatggttt catccttcgc ccgttcctgt
    44281 atggtggcgc actgctggga ttttctggcg cattgttgtc attaattttg tcagaaattc
    44341 tggtgctgcg attgtcatcg gcggttgcgg aagtggcaca ggttttcgga acgaagtttg
    44401 atatcaatga tttatcgttc gatgaatgcc tgctattgct gctggtatgc tcgatgattg
    44461 gctgggtggc agcgtggctt gccacggtac aacatttacg ccactttacg cctgaataat
    44521 aaaagcgtgt tatactcttt ccctgcaatg ggttccgtag cagggaaaga gatcccgttg
    44581 tctcttcccg gtatttcatc tctatgtcac attttgtgcg taatttattc acaagcttgc
    44641 attgaacttg tggataaaat cactgtctga taaaacagtg gatgataacc tcgttgctct
    44701 taagctctgg cacagttgtt gctaccactg aagcgccaga agatatcgat tgagaggatt
    44761 tgaatgactg acaaaatgca aagtttagct ttagccccag ttggcaacct ggattcctac
    44821 atccgggcag ctaacgcgtg gccgatgttg tcggctgacg aggagcgggc gctggctgaa
    44881 aagctgcatt accatggcga tctggaagca gctaaaacgc tgatcctgtc tcacctgcgg
    44941 tttgttgttc atattgctcg taattatgcg ggctatggcc tgccacaggc ggatttgatt
    45001 caggaaggta acatcggcct gatgaaagca gtgcgccgtt tcaacccgga agtgggtgtg
    45061 cgcctggtct ccttcgccgt tcactggatc aaagcagaga tccacgaata cgttctgcgt
    45121 aactggcgta tcgtcaaagt tgcgaccacc aaagcgcagc gcaaactgtt cttcaacctg
    45181 cgtaaaacca agcagcgtct gggctggttt aaccaggatg aagtcgaaat ggtggcccgt
    45241 gaactgggcg taaccagcaa agacgttcgt gagatggaat cacgtatggc ggcacaggac
    45301 atgacctttg acctgtcttc cgacgacgat tccgacagcc agccgatggc accggtgctc
    45361 tatctgcagg ataaatcatc taactttgcc gacggcatcg aagatgataa ctgggaagag
    45421 caggcggcaa accgtctgac cgacgcgatg caaggtctgg acgaacgcag ccaggacatc
    45481 atccgcgcgc gctggctgga cgaagacaac aagtccacgt tgcaggaact ggctgaccgt
    45541 tacggtgttt ccgctgaacg tgtacgccag ctggaaaaga acgcgatgaa aaaactgcgc
    45601 gctgctatag aagcgtaatt tccgctatta agcagagaac cctggatgcg agtccggggt
    45661 ttttgttttt tgggcctcta caataatcaa tttcccctcc ggcaaaacgc caatccccac
    45721 gcagattgtt aataaactgt caaaatagct attccaatat cataaaaatc gggtatgttt
    45781 tagcagagta tgctgctaaa gcacgggtag ccatgcataa aacgaaataa agtgctgaac
    45841 aacaacatca caacacacgt aataaccaga agaatgggga ttctcaggat gaacataaag
    45901 ggtaaagcgt tactggcagg atgtatcgcg ctggcattca gcaatatggc tctggcagaa
    45961 gatattaaag tcgcggtcgt gggcgcaatg tccggtccgg ttgcgcagta cggtgaccaa
    46021 gagtttaccg gcgcagagca ggcggttgcg gatatcaacg ctaaaggcgg cattaaaggc
    46081 aacaaactgc aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc ggttgcggtg
    46141 gcgaacaaag tcgttaacga cggcattaaa tatgtgattg gtcacctctg ttcttcatca
    46201 acgcagcctg cgtctgacat ctacgaagac gaaggcattt taatgatcac cccagcggca
    46261 accgcgccgg agctgaccgc ccgtggctat cagctgatcc tgcgcaccac cggcctggac
    46321 tccgaccagg ggccgacggc ggcgaaatat attcttgaga aagtgaaacc gcagcgtatt
    46381 gctatcgttc acgacaaaca gcaatacggc gaaggtctgg cacgagcggt gcaggacggc
    46441 ctgaagaaag gcaatgctaa cgtggtgttc tttgatggta tcaccgccgg ggaaaaagat
    46501 ttctctacgc tggtggcgcg tctgaagaaa gagaatatcg acttcgttta ctacggcggt
    46561 taccacccgg aaatggggca aatcctgcgt caggcacgcg cggcagggct gaaaactcag
    46621 tttatggggc cggaaggtgt ggctaacgtt tcgctgtcta acattgcggg cgaatcagcg
    46681 gaagggctgc tggtgaccaa gccgaagaac tacgatcagg ttccggcaaa caaacccatt
    46741 gttgacgcga tcaaagcgaa aaaacaggac ccaagcggcg cattcgtttg gaccacctac
    46801 gccgcgctgc aatctttgca ggcgggcctg aatcagtctg acgatccggc tgaaatcgcc
    46861 aaatacctga aagcgaactc cgtggatacc gtaatgggac cgctgacgtg ggatgagaaa
    46921 ggcgatctga aaggctttga gttcggcgta tttgactggc acgccaacgg cacggcgaca
    46981 gatgcgaagt aatcattaat cggcaacttt gggttgccgc caaattgcta atatcgagta
    47041 cgttgcttca tgccggatgc ggcgtaaacg ccttatccgg cctataagat ccaaagaaat
    47101 cagtaaattg caacacacat tgtaggcctg ataagcgtag cgcatcaggc aatttctcgt
    47161 tggtcatcac ttttgaaatc aacttgacga ttaacgcttc tcccagccgc cctgttgcgc
    47221 cgtaaatccc agtgcctgca taaacgctgt catcacaccg cgatcttcca cgcctgcatc
    47281 cgccatccac cagcatgaaa cgccaggatt gttacgcaaa acctcttcca gcagatattg
    47341 ccccacaccg cgacgtcggg tgacttcccg cacacgcagg gaatccagtg ctccctcggt
    47401 gccgcttaag gttacccgta cggcagcgag caggcgctcg ttaaaacgcg cggcgtagat
    47461 acggtggtta tcgtcaacct gtaaagagga aggggaatac tccggccaga tcttttgcag
    47521 gtcaatccgg tcctggtcgc taaagttttc taatcgaatg atggtcagct tcatgggtaa
    47581 cccgtgtaaa tcacaaaagt ggaaccagtg tagcgaaata atttaatcgg aggctttctc
    47641 ttttttattt cttttggcag gtgattaatt ttttaacagc aataattaca aagttaaaac
    47701 attagagaat gaaaaatacc cagcataatc ccctgaatgg tagtgaatta ttccgccctt
    47761 tgtgccgtta ttttatgctg acaaaggcac ttttttctgt ttatctatca ataaattcag
    47821 aatattatct gttcttaatc gactgaaaaa tagggatttt aatcactgtt atcacaaaat
    47881 actgcgctaa acccttaatc agacaggcaa aaacagtgca gtataaaaaa agaacagtct
    47941 gatttgttaa cacataaaaa caaagcaaca caacatcacg aatggggatt tttgactatg
    48001 aaacggaatg cgaaaactat catcgcaggg atgattgcac tggcaatttc acacaccgct
    48061 atggctgacg atattaaagt cgccgttgtc ggcgcgatgt ccggcccgat tgcccagtgg
    48121 ggcgatatgg aatttaacgg cgcgcgtcag gcgattaaag acattaatgc caaaggggga
    48181 attaagggcg acaagctggt tggcgtggaa tatgacgacg cctgcgaccc gaaacaagcc
    48241 gttgcggtcg ccaacaaaat cgttaatgac ggcattaaat acgttattgg tcatctgtgt
    48301 tcttcttcta cccaacctgc atcagatatc tacgaagacg aaggtattct gatgatctcg
    48361 ccgggagcga ccaacccgga gctgacccaa cgcggttatc aacacattat gcgtactgcc
    48421 gggctggact cttcccaggg gccaacggcg gcaaaataca ttcttgagac ggtgaagccc
    48481 cagcgcatcg ccatcattca cgacaaacaa cagtatggcg aagggctggc gcgttcggtg
    48541 caggacgggc tgaaagcggc taacgccaac gtcgtcttct tcgacggtat taccgccggg
    48601 gagaaagatt tctccgcgct gatcgcccgc ctgaaaaaag aaaacatcga cttcgtttac
    48661 tacggcggtt actacccgga aatggggcag atgctgcgcc aggcccgttc cgttggcctg
    48721 aaaactcagt ttatggggcc ggaaggtgtg ggtaacgcat cattgtcgaa tattgctggc
    48781 gatgctgccg aaggcatgtt ggtcactatg ccaaaacgct atgaccagga tccggcaaac
    48841 cagggcatcg ttgatgcgct gaaagcagac aagaaagatc cgtccgggcc ttatgtctgg
    48901 atcacctacg cggcggtgca atctctggcg actgcccttg agcgtactgg cagcgatgag
    48961 ccgctggcgc tggtgaaaga tttaaaagct aacggtgcaa acaccgtgat tgggccgctg
    49021 aactgggatg aaaaaggcga tcttaaggga tttgattttg gtgtcttcca gtggcacgcc
    49081 gatggttcat ccacggcagc caagtgatca tcccaccgcc cgtgaaatgc gggcgggttt
    49141 agaaaggtta ccttatgtct gagcagtttt tatatttctt gcagcagatg tttaacggcg
    49201 tcacgctggg cagtacctac gcgctgatag ccatcggcta caccatggtt tacggcatta
    49261 tcggcatgat caacttcgcc cacggcgagg tttacatgat tggtagctat gtctccttta
    49321 tgatcatcgc cgcactgatg atgatgggca ttgataccgg ctggctgctg gtggcggcgg
    49381 gattcgtcgg cgcaatcgtc attgccagcg cctacggctg gagtatcgaa cgggtagcct
    49441 atcgcccggt gcgtaactct aagcgcctga ttgcgctcat ctcggcaatc ggcatgtcca
    49501 tcttcctgca aaactacgtt agcctgaccg aaggttcgcg tgacgtggcg ctgcccagtc
    49561 tgtttaacgg tcagtgggtg gtggggcata gcgaaaactt ctctgcctct attaccacca
    49621 tgcaggcagt gatctggatt gttactttcc tcgccatgct ggcgctgacg attttcattc
    49681 gttattcccg tatggggcgc gcctgccgtg cctgcgcgga agatctgaaa atggcgagcc
    49741 tgcttggcat caacaccgac cgggtgattg ctttgacctt tgttattggc gcggcgatgg
    49801 cggcagtggc tggcgtactg ctcggtcagt tctacggcgt cattaacccc tatatcggct
    49861 ttatggccgg gatgaaagcc tttaccgctg cggtgctcgg tgggattggc agcattccag
    49921 gggcgatgat tggcgggctg atcctgggga ttgcggaggc gctctcttct gcctatctga
    49981 gtacggaata taaagatgtg gtctcattcg ccctgctgat tctggtgctg ctggtgatgc
    50041 cgaccggtat tctgggtcgc ccggaggtag agaaagtatg aaaccgatgc atattgcaat
    50101 ggcactgctc tctgccgcga tgttctttgt cctcgctggc gtctttatgg gcgtgcaact
    50161 ggagctggat ggcaccaaac tggtggtcga cagcgcttcg gatgtccgtt ggcagtgggt
    50221 gtttatcggc acggcggtgg tctttttctt ccagcttttg cgacctgctt tccagaaagg
    50281 gctaaaaagc gtttccgggc cgaagtttat tctgcccgcg attgatggct ccacggtgaa
    50341 gcagaaactg ttcctcgtgg cgctgctggt gcttgcggtg tcgtggccgt ttatggtgtc
    50401 acgtgggacg gtggatatcg ccactctgac catgatctac attatcctcg gtctcgggct
    50461 gaatgtggtt gttggtcttt ctggtctgct ggtgctgggg tacggcggtt tttacgccat
    50521 cggcgcttac acttttgcgc tgctcaatca ctattacggt ctggggttct ggacctgttt
    50581 gccgattgcc ggactgatgg cagcggcggc gggcttcctg cttggttttc cggtgttgcg
    50641 tctgcgcggt gattatctgg cgatcgttac cctcggtttc ggcgaaattg tgcgcatatt
    50701 gctgctcaat aacaccgaaa ttaccggcgg cccgaacggt atcagtcaga tcccgaaacc
    50761 gactttcttc ggccttgagt tcagccgtac cgcccgtgaa ggcggttggg acacgttcag
    50821 taatttcttt ggcctgaaat acgatccctc cgatcgcgtc atcttcctgt atctggtggc
    50881 gttgctgctg gtggtgctaa gcctgtttgt cattaaccgc ctgctgcgga tgccgctggg
    50941 gcgtgcgtgg gaagcgttgc gtgaagatga gatcgcctgc cgttcgctgg gcttaagccc
    51001 gcgtcgtatc aagctgactg cctttaccat cagtgccgcg tttgccggtt ttgccggaac
    51061 gctgtttgct gcgcgccagg gctttgtcag cccggaatcc ttcacctttg ctgaatcggc
    51121 gtttgtactg gcgatagtgg tgctcggcgg tatgggatcg cagtttgcgg tgattctggc
    51181 ggcaattttg ctggtggtat cgcgcgaatt gatgcgtgat ttcaacgaat acagcatgtt
    51241 aatgctcggt ggtttgatgg tgctgatgat gatctggcgt ccgcagggct tgctgcccat
    51301 gacgcgcccg caactgaagc tgaaaaacgg cgcagcgaaa ggagagcagg catgagtcag
    51361 ccattattat ctgtgaacgg cctgatgatg cgcttcggcg gcctgctggc ggtgaacaac
    51421 gtcaatcttg aactgtaccc gcaggagatc gtctcgttaa tcggccctaa cggtgccgga
    51481 aaaaccacgg tttttaactg tctgaccgga ttctacaaac ccaccggcgg caccatttta
    51541 ctgcgcaatc agcacctgga aggtttaccg gggcagcaaa ttgcccgcat gggcgtggtg
    51601 cgcaccttcc agcatgtgcg tctgttccgt gaaatgacgg taattgaaaa cctgctggtg
    51661 gcgcagcatc aacaactgaa aaccgggctg ttctctggcc tgttgaaaac gccttctttc
    51721 cgtcgcgccc agagcgaagc gctcgaccgc gccgccacct ggcttgagcg cattggtttg
    51781 ctggaacacg ccaaccgtca ggcgagtaac ctggcctatg gtgaccaacg ccgtctggag
    51841 attgcccgct gcatggtgac gcagccggag attttaatgc tcgacgaacc ggcggcaggt
    51901 cttaacccaa aagaaaccaa agagctggat gagctgattg ccgaactgcg caatcatcac
    51961 aacaccacta tcttgttgat tgaacacgat atgaagctgg tgatggggat ttcagaccga
    52021 atttatgtgg tcaatcaggg gacgccactg gcaaacggta cgccggagca aatccgtaat
    52081 aacccggacg tgatccgtgc ctatttaggt gaggcataag atggaaaaag tcatgttgtc
    52141 ctttgacaaa gtcagcgccc actacggcaa aatccaggcg ctgcatgagg tgagcctgca
    52201 tatcaatcag ggcgagattg tcaccctcat tggcgcgaac ggggcgggga aaaccacctt
    52261 gctcggcacg ttatgcggcg atccgcgcgc taccagcggg cgaattgtgt ttgatgataa
    52321 agacattacc gactggcaga cggcgaaaat catgcgcgaa gcggtagcga ttgtcccgga
    52381 agggcgtcgc gtcttctcgc ggatgacggt ggaagagaac ctggcgatgg gcggcttttt
    52441 tgccgaacgc gaccagttcc aggagcgcat aaagtgggtg tatgaactgt tcccacgtct
    52501 gcatgagcgc cgtgttcagc gggcaggcac catgtccggc ggtgagcaac aaatgctggc
    52561 gattggtcgc gcgctgatga gcaacccgcg tttgctgcta cttgatgagc catcgcttgg
    52621 tcttgcgccg attatcatcc agcaaatttt cgacaccatc gagcagctgc gcgagcaggg
    52681 gatgactatc ttcctcgtcg agcagaacgc caaccaggcg ctaaagctgg cggatcgcgg
    52741 ctacgtgctg gaaaacggcc atgtggtgct ctccgatact ggtgatgcac tactggcgaa
    52801 tgaagcggtg agaagtgcgt atttaggcgg gtaataacac gttgattgat aaggagtcaa
    52861 aagactcctt tgagacaggt gacaaatgta aaattgcctg atgcgctgcg cttatcaggc
    52921 ctactgggtg agttgcaata tattgaattt gcacgatctt gtaggccgga taaggcgttt
    52981 acgccgcatc cggcatgaaa cgatgagtaa tctgtagagt ttgattcaga ccttctattt
    53041 ttccgcttat ccgtgcccca tctcccattt tccctcaccc acgccgtcac cgccttgtca
    53101 tctttctgac accttacttg caggtttatg aagttggcac cgccaccatg atggcatcga
    53161 aagccattaa accggtatat gacgtgttta aagaggcagg gattcaattc gatgagtcgc
    53221 agtttgtgcc gacggtttcc ggttactact ccgacagcaa aacgggccac ttactctccc
    53281 agccattcaa cagctcgact cccgttctct attacaacaa agacgccttc aagaaagcgg
    53341 ggttagaccc ggaacagccg ccgaaaacct ggcaggatct ggcggactct tccgcaaaac
    53401 tgaaagcctc cggtatgaag tgcggctacg ccagcggctg gcagggctgg atccaactgg
    53461 aaaactttag cgcctggaac ggtctgccgt ttgccagcaa aaacaacggc tttgacggca
    53521 cggacgcggt gctggagttc aacaagccgg agcaggtgaa acacattgcc atgctcgaag
    53581 agatgaacaa gaagggcgac ttcagctacg tcggtcgtaa ggatgaatcc accgagaagt
    53641 tctataacgg tgattgcgcg atgaccaccg cctcttccgg ttctctcgct aatattcgtg
    53701 agtacgccaa atttaactat ggcgtaggca tgatgcctta cgacgccgat gcgaaagatg
    53761 caccgcaaaa cgccattatc ggcggggcca gcctgtgggt gatgcagggc aaaaataaag
    53821 aaacgtatac cggcgtggcg aagttcctcg acttcctcgc gaagccagaa aacgctgccg
    53881 agtggcatca gaaaaccggt tatctgccaa tcaccaaagc agcgtatgac ctgacccgtg
    53941 agcagggctt ttatgagaaa aacccagggg cggataccgc gacgcgtcag atgctgaata
    54001 agccgccgtt gccgttcacc aaagggctgc gtctgggcaa catgccgcag atccgcgtga
    54061 ttgtggatga agagctggag agcgtgtgga ccggtaagaa gacaccacag caggcactgg
    54121 ataccgccgt tgagcgtgga aatcagttgc tgcgccgctt tgagaaatcg acgaagtctt
    54181 aatcagtgta atgtcggatg cgtttcgctt atctgacctg gcatcgcgtg taggccggat
    54241 aagcgaagcg catccggcac agttcaggaa ttaaccgtaa tgtcatcatc ccgtccggtg
    54301 ttccgctcgc gctggctgcc ttatctgctg gtcgcgccgc agctcatcat caccgttatc
    54361 ttttttatct ggcctgcggg cgaagcgttg tggtactcgc ggcaaagcgt cgatccgttt
    54421 ggtttctcca gccagtttgt cggtctggat aacttcgtca cgctgttcca tgacagctac
    54481 tatctagact ccttctggac gacgataaaa ttcagcacct ttgtcaccgt cagcggtttg
    54541 ctggtatcgc tgttctttgc ggcgctggtg gagtacatcg tgcgcggcag ccgtttctat
    54601 caaaccttga tgctgctgcc gtatgccgtg gctcccgccg ttgccgccgt attgtggatc
    54661 ttcctgttta accccggtcg cgggctgatc acccattttc tcgctgagtt cggctacgac
    54721 tggaaccacg cgcaaaacag cggtcaggca atgtttctgg tggtgtttgc ctcggtgtgg
    54781 aagcaaatca gctacaactt tctgttcttc tatgccgcgc tgcactccat tccccgttcg
    54841 ttgattgaag ccgcagctat tgacggcgca gggccgattc gccgcttctt taagattgct
    54901 ctgccgctta tcgctccggt gagtttcttc ctgctggtgg tgaacctggt gtacgccttc
    54961 ttcgacacct tcccggtgat cgacgccgcc acgtccggcg ggccggttca ggccaccact
    55021 acgctgattt ataagatcta ccgcgaaggc tttaccggac tggatctggc ttcgtctgcc
    55081 gcgcagtcgg tggtgttgat gttcctcgtc atcgtgctga cggtggtgca gttccgctat
    55141 gttgaaagca aggtgcgtta ccaatgattg agaaccgtcc gtggctgacg atattcagcc
    55201 ataccatgct gatcctcggg atcgcggtga tcctcttccc gctgtacgtg gcgtttgtcg
    55261 cggcgacgct ggataaacag gccgtctatg ccgcgccgat gacgctcatc cccggcacac
    55321 atctgctgga aaatatccac aacatctggg ttaacggggt aggcacgaat agcgcgccgt
    55381 tctggcggat gttgcttaac agctttgtga tggcgttcag cattacgctc ggcaaaatta
    55441 ccgtctcgat gctctcggca tttgccattg tctggtttcg ttttccgcta cgtaacctct
    55501 tcttctggat gatttttatc accctgatgc tgccggttga agtacgtatc ttcccgacgg
    55561 tggaagtcat cgccaacctg aaaatgctcg acagctacgc cggtttaacg ctgccgctga
    55621 tggcctcggc gaccgccacg tttctgttcc gccagttctt tatgacccta ccggatgaat
    55681 tggtggaagc cgcgcggatc gacggcgcgt caccaatgcg tttcttttgc gacatcgttt
    55741 ttccgttgtc caaaactaat ctggcggcgc tgtttgtgat cacctttatc tacggctgga
    55801 atcagtattt gtggccgttg ttgattatta ccgacgttga tctcggcacc accgtggcag
    55861 ggatcaaagg gatgatcgct acaggcgaag gcaccacgga atggaactcg gtaatggcgg
    55921 cgatgttgtt aacgcttatc cctccggtgg tgattgtttt agtgatgcag cgtgccttcg
    55981 tgcgcggcct ggtcgatagt gagaaataag atggcaggac tgaaattaca ggcagtaacc
    56041 aaaagctggg atggcaaaac ccaggtcatt aaaccgctga cccttgatgt ggcggatggc
    56101 gaatttatcg tgatggtcgg gccgtctggc tgcgggaaat cgacgctgct gcgcatggtt
    56161 gccgggctgg agcgggtgac agaaggcgat atctggatca acgaccagcg cgtgaccgaa
    56221 atggagccga aagatcgcgg gattgcgatg gtgttccaga attacgcgct ttatccgcat
    56281 atgagcgtag aggagaacat ggcgtggggg ctgaaaattc gcggcatggg caagcagcaa
    56341 attgccgagc gcgttaaaga agcggcgcgc attctggaac tggacggtct gcttaagcgt
    56401 cgtccgcgtg agctatccgg cggtcagcgc cagcgtgtgg cgatgggccg cgcgattgtg
    56461 cgcgatccgg cggtgttctt gtttgatgag ccgctctcta acctcgatgc caagctgcgc
    56521 gtgcagatgc gtcttgaact gcaacagttg caccgtcgcc tgaaaacgac ttcactctac
    56581 gttactcacg atcaggttga agcgatgacg ctcgcccagc gagtaatggt gatgaacggt
    56641 ggcgttgccg aacagattgg cacaccagtt gaagtctacg aaaagcccgc cagcctgttt
    56701 gtggcgagtt ttatcggcag cccggcgatg aacctgctga caggccgcgt gaataatgaa
    56761 ggcacgcatt tcgaactgga cggcggtatc gtgctaccgc taaacggtgg ctaccgtcag
    56821 tatgccgggc gtaaaatgac tctcggcatt cgcccggaac atatagcgct aagctcgcag
    56881 gcagaaggtg gtgtgccgct ggtgatggac acgctggaga tcctcggcgc agataacctg
    56941 gcgcacggac gctggggcga gcagaagctg gtggtgcgac tggcgcatca ggagcgcccg
    57001 acggcaggca gcacgctgtg gctgcatttg gcggaaaatc agctgcatct ttttgatggt
    57061 gaaacaggac aacgagtatg agtaactggc cttatccccg catcgtcgct catcgtggcg
    57121 gcggtaagct ggccccggaa aacaccctgg cggcaatcga cgtcggggca aaatacggtc
    57181 ataagatgat cgaatttgac gcgaagttat cgaaagatgg cgagatcttc ctgctccatg
    57241 acgacaatct cgaacgcacc agcaacggct ggggcgtcgc gggtgaactg aactggcagg
    57301 atttactgcg cgtggatgcg ggcagttggt acagcaaagc gtttaaaggt gagccgctgc
    57361 cgttgctttc gcaggtggcg gaacgctgtc gcgaacacgg gatgatggcg aatatcgaaa
    57421 tcaaacccac cactggcact ggaccattaa cgggcaaaat ggtggcgctg gcggcacgcc
    57481 aactgtgggc cggtatgacg ccgccgctgc tgtcatcgtt tgagattgat gctttagaag
    57541 ctgcacaaca ggcggcaccg gaactgccgc gtggtttatt gcttgatgag tggcgcgacg
    57601 actggcgcga actgaccgcg cggctgggct gcgtctctat tcatctcaat cataagttac
    57661 tcgataaagc gcgagtgatg cagttgaaag acgccggatt acggattctg gtttataccg
    57721 tcaataaacc ccagcgcgcg gcagagttgc tgcgctgggg cgtggattgc atctgtaccg
    57781 atgcgattga cgtgattggt ccgaacttta cggcccaata gttttcaacg gaatgtcagg
    57841 ctgcggcgtg ctgggctgat tcagcatgtc gccgtttctc ctttccggca gcatatgctg
    57901 ctgattcaac gaactatccg gattacggtt gctgtttaac atcccgccgt tggtgttggg
    57961 cagcatttgc tgccgggcgg gatttcgctc ccccggctgt gactgcaaca cccgctgaga
    58021 attattgttt atctggtttt ctaaatgctg ctgttgcaac tgagtttgcg ttttcagttg
    58081 ctgattcagc atcccttttt gctggatttg ctgagtctgc atctgggttt gcatccgctg
    58141 ctggctgggt atctgatacc ccggctggtt agggttgttc agagtattaa tgggctgtgc
    58201 aaagccgaca aacggcagga gtgccgtaag aatcagaagt cgtttcatcg cgtatcctcc
    58261 tctgaagata tcctttaagt ttactcgctt cccgacaaaa cgatgattaa ttcagagtta
    58321 tataccaggc ttagctgggg ttgcccctta atctctggag aataacgatg ataaaaccga
    58381 cgtttttacg ccgggtggcc attgctgctc tgctctcagg aagttgtttt agcgccgccg
    58441 ccgcgcctcc tgcgccgccc gtctcgtatg gtgtggagga agatgtcttc cacccggtac
    58501 gcgcgaaaca gggaatggta gcgtctgtgg acgccactgc cactcaggtg ggggtggata
    58561 tcctcaagga gggcgggaat gccgttgatg ccgccgtggc ggtgggctac gcgctggcgg
    58621 taacgcatcc gcaggcaggg aatctgggcg gtggtggttt tatgttaatc cgctcgaaaa
    58681 atggcaatac cacggctatc gatttccgcg aaatggcacc cgccaaagcg acccgcgata
    58741 tgttcctcga tgatcagggc aacccggaca gcaaaaaatc actcacttcg catctggctt
    58801 ccggcacacc gggtacggta gcaggtttct cgctggcgct ggataaatac ggcaccatgc
    58861 cgctgaacaa agtcgtgcag cccgcgttta aactggcacg cgatggtttt atcgttaacg
    58921 acgcgctggc tgacgatctc aaaacctacg gtagcgaagt gttgccgaat cacgaaaaca
    58981 gtaaagctat cttctggaaa gagggcgagc cgctgaaaaa gggcgacacg ctggtgcagg
    59041 cgaacctggc aaagagcctg gagatgattg ctgaaaacgg cccggacgaa ttctataaag
    59101 gcacgattgc ggaacagatc gcccaggaga tgcagaaaaa cggtggcttg atcactaaag
    59161 aagatttagc ggcctataaa gcggtcgaac gcactccgat aagcggcgat tatcgcgggt
    59221 atcaggttta ctccatgcca ccgccatcct ccggcgggat ccatatcgta caaatcctca
    59281 atattctgga aaacttcgat atgaagaaat acggctttgg cagcgccgat gcgatgcaaa
    59341 tcatggcaga agcggagaaa tacgcctacg ccgaccgctc ggaatatctt ggcgacccgg
    59401 attttgtcaa agtcccgtgg caggcactga ccaataaagc ctatgccaaa tccattgccg
    59461 atcaaattga tatcaacaaa gcgaagccgt ccagtgagat tcgccctggc aagcttgcgc
    59521 cttatgagag taatcaaact acccattact ctgtagtgga taaagacggt aacgcggtgg
    59581 cggtgaccta tacgctgaac accaccttcg gtacgggcat tgtcgcgggc gagagcggta
    59641 ttctgcttaa taatcagatg gatgatttct ccgccaaacc gggcgtaccg aacatttacg
    59701 ggctggtggg cggtgatgcc aacgccgtcg ggccgaataa acgtccgctg tcatcgatgt
    59761 caccaaccat tgtggtgaaa gacggtaaaa cttggctggt taccggtagt cctggcggga
    59821 gtcggattat taccaccgtg c
//
LOCUS       DAETQN010000010        58572 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.010, whole genome shotgun sequence.
ACCESSION   DAETQN010000010 DAETQN010000000
VERSION     DAETQN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58572
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.010"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..153
                     /locus_tag="K0W68_000666"
     CDS             <1..153
                     /locus_tag="K0W68_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBI3072321.1"
                     /translation="LRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQGS"
     gene            182..346
                     /locus_tag="K0W68_000667"
                     /pseudo
     CDS             182..346
                     /locus_tag="K0W68_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            complement(384..1445)
                     /locus_tag="K0W68_000668"
     CDS             complement(384..1445)
                     /locus_tag="K0W68_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBI3072322.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1658..3121
                     /gene="bepA"
                     /locus_tag="K0W68_000669"
     CDS             1658..3121
                     /gene="bepA"
                     /locus_tag="K0W68_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBI3072323.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            3142..3501
                     /gene="arsC"
                     /locus_tag="K0W68_000670"
     CDS             3142..3501
                     /gene="arsC"
                     /locus_tag="K0W68_000670"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBI3072324.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3639..4385)
                     /gene="hda"
                     /locus_tag="K0W68_000671"
     CDS             complement(3639..4385)
                     /gene="hda"
                     /locus_tag="K0W68_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBI3072325.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4435..5724)
                     /gene="uraA"
                     /locus_tag="K0W68_000672"
     CDS             complement(4435..5724)
                     /gene="uraA"
                     /locus_tag="K0W68_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBI3072326.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5810..6436)
                     /gene="upp"
                     /locus_tag="K0W68_000673"
     CDS             complement(5810..6436)
                     /gene="upp"
                     /locus_tag="K0W68_000673"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBI3072327.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6761..7798
                     /gene="purM"
                     /locus_tag="K0W68_000674"
     CDS             6761..7798
                     /gene="purM"
                     /locus_tag="K0W68_000674"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBI3072328.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7798..8436
                     /gene="purN"
                     /locus_tag="K0W68_000675"
     CDS             7798..8436
                     /gene="purN"
                     /locus_tag="K0W68_000675"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBI3072329.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8607..10673
                     /gene="ppk1"
                     /locus_tag="K0W68_000676"
     CDS             8607..10673
                     /gene="ppk1"
                     /locus_tag="K0W68_000676"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBI3072330.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10678..12219
                     /gene="ppx"
                     /locus_tag="K0W68_000677"
     CDS             10678..12219
                     /gene="ppx"
                     /locus_tag="K0W68_000677"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBI3072331.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12258..14501)
                     /gene="pdeF"
                     /locus_tag="K0W68_000678"
     CDS             complement(12258..14501)
                     /gene="pdeF"
                     /locus_tag="K0W68_000678"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBI3072332.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14683..14835)
                     /locus_tag="K0W68_000679"
     CDS             complement(14683..14835)
                     /locus_tag="K0W68_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072333.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14853..15044
                     /locus_tag="K0W68_000680"
     CDS             14853..15044
                     /locus_tag="K0W68_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBI3072334.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15355..15873
                     /locus_tag="K0W68_000681"
     CDS             15355..15873
                     /locus_tag="K0W68_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBI3072335.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15889..16428
                     /locus_tag="K0W68_000682"
     CDS             15889..16428
                     /locus_tag="K0W68_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBI3072336.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16523..18100)
                     /gene="guaA"
                     /locus_tag="K0W68_000683"
     CDS             complement(16523..18100)
                     /gene="guaA"
                     /locus_tag="K0W68_000683"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBI3072337.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(18169..19635)
                     /gene="guaB"
                     /locus_tag="K0W68_000684"
     CDS             complement(18169..19635)
                     /gene="guaB"
                     /locus_tag="K0W68_000684"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBI3072338.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19797..21167
                     /gene="xseA"
                     /locus_tag="K0W68_000685"
     CDS             19797..21167
                     /gene="xseA"
                     /locus_tag="K0W68_000685"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBI3072339.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(21164..21379)
                     /locus_tag="K0W68_000686"
     CDS             complement(21164..21379)
                     /locus_tag="K0W68_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBI3072340.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21448..22920)
                     /gene="der"
                     /locus_tag="K0W68_000687"
     CDS             complement(21448..22920)
                     /gene="der"
                     /locus_tag="K0W68_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBI3072341.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(23038..24216)
                     /gene="bamB"
                     /locus_tag="K0W68_000688"
     CDS             complement(23038..24216)
                     /gene="bamB"
                     /locus_tag="K0W68_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBI3072342.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24227..24847)
                     /gene="yfgM"
                     /locus_tag="K0W68_000689"
     CDS             complement(24227..24847)
                     /gene="yfgM"
                     /locus_tag="K0W68_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBI3072343.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24865..26139)
                     /gene="hisS"
                     /locus_tag="K0W68_000690"
     CDS             complement(24865..26139)
                     /gene="hisS"
                     /locus_tag="K0W68_000690"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBI3072344.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26250..27368)
                     /gene="ispG"
                     /locus_tag="K0W68_000691"
                     /gene_synonym="gcpE"
     CDS             complement(26250..27368)
                     /gene="ispG"
                     /locus_tag="K0W68_000691"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBI3072345.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27395..28408)
                     /gene="rodZ"
                     /locus_tag="K0W68_000692"
     CDS             complement(27395..28408)
                     /gene="rodZ"
                     /locus_tag="K0W68_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBI3072346.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28693..29847)
                     /gene="trmG/rlmN"
                     /locus_tag="K0W68_000693"
     CDS             complement(28693..29847)
                     /gene="trmG/rlmN"
                     /locus_tag="K0W68_000693"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBI3072347.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29997..30428)
                     /gene="ndk"
                     /locus_tag="K0W68_000694"
     CDS             complement(29997..30428)
                     /gene="ndk"
                     /locus_tag="K0W68_000694"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBI3072348.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30577..32889)
                     /gene="pbpC"
                     /locus_tag="K0W68_000695"
     CDS             complement(30577..32889)
                     /gene="pbpC"
                     /locus_tag="K0W68_000695"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HBI3072349.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32890..37851)
                     /gene="yfhM"
                     /locus_tag="K0W68_000696"
     CDS             complement(32890..37851)
                     /gene="yfhM"
                     /locus_tag="K0W68_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HBI3072350.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNCLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            38058..38903
                     /gene="sseA"
                     /locus_tag="K0W68_000697"
     CDS             38058..38903
                     /gene="sseA"
                     /locus_tag="K0W68_000697"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HBI3072351.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(39396..40172)
                     /gene="sseB"
                     /locus_tag="K0W68_000698"
     CDS             complement(39396..40172)
                     /gene="sseB"
                     /locus_tag="K0W68_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBI3072352.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(40314..41597)
                     /gene="pepB"
                     /locus_tag="K0W68_000699"
     CDS             complement(40314..41597)
                     /gene="pepB"
                     /locus_tag="K0W68_000699"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBI3072353.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(41775..41975)
                     /gene="iscX"
                     /locus_tag="K0W68_000700"
     CDS             complement(41775..41975)
                     /gene="iscX"
                     /locus_tag="K0W68_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBI3072354.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(41987..42322)
                     /gene="fdx"
                     /locus_tag="K0W68_000701"
     CDS             complement(41987..42322)
                     /gene="fdx"
                     /locus_tag="K0W68_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBI3072355.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(42324..44174)
                     /gene="hscA"
                     /locus_tag="K0W68_000702"
     CDS             complement(42324..44174)
                     /gene="hscA"
                     /locus_tag="K0W68_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBI3072356.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(44191..44706)
                     /gene="hscB"
                     /locus_tag="K0W68_000703"
     CDS             complement(44191..44706)
                     /gene="hscB"
                     /locus_tag="K0W68_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBI3072357.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(44802..45125)
                     /gene="iscA"
                     /locus_tag="K0W68_000704"
     CDS             complement(44802..45125)
                     /gene="iscA"
                     /locus_tag="K0W68_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBI3072358.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(45142..45528)
                     /gene="iscU"
                     /locus_tag="K0W68_000705"
     CDS             complement(45142..45528)
                     /gene="iscU"
                     /locus_tag="K0W68_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBI3072359.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(45556..46770)
                     /gene="iscS"
                     /locus_tag="K0W68_000706"
     CDS             complement(45556..46770)
                     /gene="iscS"
                     /locus_tag="K0W68_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBI3072360.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(47011..47499)
                     /gene="iscR"
                     /locus_tag="K0W68_000707"
     CDS             complement(47011..47499)
                     /gene="iscR"
                     /locus_tag="K0W68_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBI3072361.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(47800..48537)
                     /gene="trmJ"
                     /locus_tag="K0W68_000708"
     CDS             complement(47800..48537)
                     /gene="trmJ"
                     /locus_tag="K0W68_000708"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBI3072362.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            48656..49459
                     /gene="suhB"
                     /locus_tag="K0W68_000709"
     CDS             48656..49459
                     /gene="suhB"
                     /locus_tag="K0W68_000709"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBI3072363.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            49604..50458
                     /locus_tag="K0W68_000710"
     CDS             49604..50458
                     /locus_tag="K0W68_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI3072364.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            50649..51929
                     /gene="csiE"
                     /locus_tag="K0W68_000711"
     CDS             50649..51929
                     /gene="csiE"
                     /locus_tag="K0W68_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBI3072365.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(51921..53060)
                     /locus_tag="K0W68_000712"
     CDS             complement(51921..53060)
                     /locus_tag="K0W68_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBI3072366.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(53220..54110)
                     /gene="hcaR"
                     /locus_tag="K0W68_000713"
     CDS             complement(53220..54110)
                     /gene="hcaR"
                     /locus_tag="K0W68_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HBI3072367.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            54246..55607
                     /locus_tag="K0W68_000714"
     CDS             54246..55607
                     /locus_tag="K0W68_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBI3072368.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            55604..56122
                     /locus_tag="K0W68_000715"
     CDS             55604..56122
                     /locus_tag="K0W68_000715"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBI3072369.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            56122..56442
                     /locus_tag="K0W68_000716"
     CDS             56122..56442
                     /locus_tag="K0W68_000716"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBI3072370.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            56439..57251
                     /gene="hcaB"
                     /locus_tag="K0W68_000717"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             56439..57251
                     /gene="hcaB"
                     /locus_tag="K0W68_000717"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBI3072371.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            57261..58463
                     /locus_tag="K0W68_000718"
     CDS             57261..58463
                     /locus_tag="K0W68_000718"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HBI3072372.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        14316 a        15842 c        14612 g        13802 t
ORIGIN      
        1 ctcagggatc cgctctccag ggcttactac acccgcaaaa tgagtcaggg aaaacgacac
       61 aatcaggcgc ttatcgccct ggcgagacga cgctgcgacg ttctgttcgc catgatgcgc
      121 gacgggactt tttatacccc gcaggggtca taacatgctt gacaacttaa taggggcacc
      181 ccccttctgg caactggcgc acagtagcgc agacaatttt ccggcactga cggtaagcca
      241 ttttattacc gccaatctgc tcccggtgat gctgggtaat attatcggcg gtgcagtgct
      301 ggtgagtatg tgttatcggg ctatttattt acgtcaggaa tcctgaaaaa tcagcccggc
      361 gaaacagttc gtcgggctga agattattct tgcgctattt gcccatcggg ccaggcgtgg
      421 atcacggctt tgatcagcgt cgccaatgga atagcgaaga atacgcccca gaatccccac
      481 aaaccaccga agatcaccac cgataaaata atcaccagcg gatgcaggtt aaccgcttcg
      541 gagaacaaca ccggtactaa cagattaccg tccagcgcct gaataatcag atacactgcg
      601 aagcagctcc agaattccgt gcctgcacca aactggaata gcgccacgcc aaccaccgga
      661 atggtcacca caaatgcgcc aatgtacgga ataagtaccg agaagccgac cagcaccgcc
      721 agcagcagcg aatagttcag cccaaagagc aagaacccca gccaagtggc gatccccacc
      781 acgatcatct ccagcacttt gccgcggata tagttggtga tttgttgatt catctccttc
      841 cacacctgtc ctgccagtcc acggttgcgc ggcagcaccc gacgaacggc gttcagcatc
      901 tgctctttgt ctttcagcag gaagaagacc atcaatggta ccagcaccag atagacggca
      961 atggtcagca aaccgaccag cgaggcgagg gaaattttca ccaccgaatc gcccatggtc
     1021 agcatccgac tgcgcatatt ttcggccatt gcatcaataa tgcccgcgtc cattaatgcc
     1081 ggatagcggc gcggcaacgt ggcggcaaag tcagaaagct tattgagcat ccccggcata
     1141 tcgcggatca agtagatgcc ctgttgccag gcgataggca gtaccacgaa cgccatcagt
     1201 agcaatatac cgacgaaaac caccaataca atcgacgtcg cccagcggcg ggagcagcca
     1261 atagattgca ggcgcacggt tggccattcc agcaaatagg ccagcacaat agccaccagc
     1321 aacggagcaa gcaggccact aaagaaaaag ataatgccaa atccggcaac taaaataacc
     1381 agcaaggcaa tcgcttccgg gtcgctaaaa cggcggcgat accattgcat caacatttcg
     1441 agcataaaga ccttccctga acctcaagag cgggattgcg atccgcaatt gtatcgaaat
     1501 gtcacaaaaa agacttcgct ttttatgacg gattcaggaa actgaaaagt catttgagtg
     1561 ggctaatctt cgccgttaca ctcaaaggcg gcgcggtggg aacaatattt cacagtatcg
     1621 gtcaaatgac tatcttcaga aatacaggat agaggttatg ttcaggcagt tgaaaaaaaa
     1681 cctggttgca accctcattg ctgctatgac cattggtcag gtagccccgg cgtttgccga
     1741 cagcgcagac accttgccgg atatgggaac ctccgcagga agcacgcttt ccattggtca
     1801 ggaaatgcag atgggcgact attatgtccg ccagctacgc ggcagcgcgc cgttaattaa
     1861 tgacccgctg ttaacgcaat atattaattc gctggggatg cgtctggttt cgcatgccaa
     1921 ttcggttaag acaccgtttc atttttttct gatcaacaac gacgaaatta acgcctttgc
     1981 tttctttggc ggcaacgtgg tgctgcactc tgccctgttc cgttattccg ataacgaaag
     2041 tcaactggct tcagttatgg cgcacgaaat ctcccacgtc acccaacgtc acctggcgcg
     2101 agcgatggaa gatcagcagc gcagcgcgcc gctgacctgg gtcggcgcgt taggttctat
     2161 tttactggcg atggccagtc cgcaggcggg gatggcggcg ctgaccggta cactggcggg
     2221 aacgcgtcag gggatgatca gtttcaccca gcaaaatgaa caggaagcgg accgcattgg
     2281 tattcaggtg ctgcaacgct cgggattcga tccgcaggcg atgccaacct tcctcgaaaa
     2341 attactcgat caggcgcgtt actcctcgcg cccgccggaa attttattga ctcacccgtt
     2401 gccggaaagt cgtctggcag atgcccgcaa ccgtgctaat cagatgcgcc cgatggtggt
     2461 gcagtcgtcg gaagatttct atctggcgaa agcgcgcaca ctggggatgt ataattccgg
     2521 acgtaaccag ctcaccagtg atttgctgga tgaatgggcg aaaggaaacg ttcgtcagca
     2581 acgagcggcg caatatggtc gtgctttaca ggcgatggaa gccaataaat acgacgaggc
     2641 gcgtaaaacg ctgcaaccgt tactggcggc agaacctggc aacgcatggt atctcgatct
     2701 ggctaccgat atcgatcttg ggcaaaacaa agccaatgag gcaatcaatc gcctgaaaaa
     2761 tgcccgtgat ttgcgcacca atccggtgtt gcagctcaac ctggcaaacg cttatctgca
     2821 aggcggtcaa ccacaagaag cggccaatat tctgaatcgc tacaccttta ataataaaga
     2881 tgacagcaac ggctgggatt tactggcaca ggcggaagcc gcgctaaata accgcgatca
     2941 ggagctggct gcgcgagcag aaggttatgc gctcgccgga cgactcgatc aggccatttc
     3001 gctgttgagt agcgccagtt cgcaggtgaa attaggcagc cagcaacaag cgcgttacga
     3061 tgcgcgcatc gaccagttgc gccagctgca ggaacgcttt aagccttata ccaagatgta
     3121 attcgcatta aaggaagaac aatgaccaaa caggtaaaaa tctaccataa cccacgctgt
     3181 tcaaagagcc gggaaacgct gaacctgctg aaaaaaaacg gcgtggagcc agaagtggta
     3241 ctctaccttg agacacccgc cgatgcggca acgctgcgcg atttgctgaa aatgctgggg
     3301 atgaatagcg cccgtgaatt gatgcgccag aaagaggatc tttataaaga actgaacctg
     3361 gctgacagtt cgcttagcga agaggcgctg attcaggcaa tggtggataa tccgaagctg
     3421 atggagcgcc cgattgtggt cgcgaatggc aaagcgcgga ttggtcgtcc accggagcag
     3481 gtactggaaa tcgtgggtta agatgtgccc catcgctagt tgaagcacat tgccggatgc
     3541 gacgcttgcc atgtcttatc cggcctacga atactatgga ttaacttcgc gtagttcgga
     3601 taaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag aatttctttc
     3661 acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa ctgatccaac
     3721 gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg ccccacatct
     3781 tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa cttatcttca
     3841 tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gtcgcgacgc gagatccggt
     3901 aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaatcg tgttttgccc
     3961 gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc atcgcctgca
     4021 atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc gagcacttcc
     4081 ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc acgctgcgac
     4141 aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg tgcccagaga
     4201 tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcagccag taaagaggag
     4261 ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag tggcaaagag
     4321 agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg cgagaagttt
     4381 accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg tacattattt
     4441 gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca gcacactaat
     4501 cagtttgaag atcaaactta agccgatacc gacgatggtc gccagcgcca tacctttcag
     4561 ctccgccgcg ccaatgttta ccttcgcgcc actgacaccg atgatcaaaa tcacggaagt
     4621 caggatcagg ttctgtgctt tgttgtaatc cacttttgat tcgatcaaaa cacgaatacc
     4681 ggaagcaccg atgacaccat aaagcagcag cgaaacgccg cccatcaccg gcaatgggat
     4741 catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga aaatcgccgc
     4801 cccaccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa tgttttctcc
     4861 gtaagtggta tttggcgtag agccaaagaa gccggagatc acagtcgaca ggccgttagc
     4921 aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga tattagccgt
     4981 tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca gaatagtcag
     5041 aatggcaaac cactcgaagc gcggcgtata gagggttggc agcgcaaacc agtgagcatt
     5101 aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc ccaccagcac
     5161 gccaattaaa atcgggataa ttgccaggaa accacggaac agcacggaac ctaaaaccgt
     5221 gaccgccagg gtggtaatag agatgatgat ggttttggag tctggcgttt gcccttcagc
     5281 cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga tgacggcaac
     5341 gattgcgccc attgccgcag gtggaaacag cacgtccagc cagccggtcc ccgctttctt
     5401 cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc ccagcgcgac
     5461 ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc tggaaccaag
     5521 ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa taccgttaaa
     5581 taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg ttgcaccaaa
     5641 catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg gtctttcact
     5701 caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta taggcgcttt
     5761 actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt tatttcgtac
     5821 caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc tcgttcagtc
     5881 cctggtcaat cgatgcggta tacagttcga cgtccgggtg cgctttttcc agcgcagcga
     5941 taccttctgg cgcagctacc agcaccagaa ctttgatgct gctgcagccc gcttttttca
     6001 gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca acgatcagcg
     6061 ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc tccagcgttt
     6121 cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacattt tccagcacac
     6181 cgtccatcat ccccagaccg gcacgcagaa ttggcacaac ggtaattttc ttacctttga
     6241 tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc gtttcgaggt
     6301 cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg cgaaagcgct
     6361 tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt gggtgtttga
     6421 cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa atcgacggat
     6481 tatacctcct ttcttcaagg cggcaatatt cttttcgttg actttagtca aaatgataac
     6541 ggtttgagat aaagttattt tatattcaaa tggttatgaa agaagattat tccatccgaa
     6601 aactaacctt tgccctggca caagtcttct ttcgccgcgc gtctggggaa aagacgtgca
     6661 aaaaggttgt gcaaagcagt ctcgaaaacg tttgctttcc ctgttagaat tgcgccgaat
     6721 tttatttttc taccgcaagt aacgcgtggg gacccaagca gtgaccgata aaacctctct
     6781 tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg gaagaatcaa
     6841 aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg gcttcggtgc
     6901 gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttccggca ctgacggcgt
     6961 aggtaccaag ctgcgtctgg caatggactt aaaacgtcac gacaccattg gtattgatct
     7021 ggtcgccatg tgcgttaatg acctggtggt gcaaggtgca gaaccgctgt ttttcctcga
     7081 ctattacgca accggaaaac tggatgttga taccgcttca gcggtgatca gcggtatcgc
     7141 ggaaggatgt ctgcaatcgg gctgttcact ggtgggtggc gaaacggcag aaatgccggg
     7201 gatgtatcac ggtgaggatt acgatgtcgc gggtttctgc gttggcgtgg tagaaaaatc
     7261 agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac tcggttccag
     7321 cggtccgcac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca gcggttgtga
     7381 tccgcaaacc accgaacttg atggtaagcc attagccgat catctgctgg caccgacccg
     7441 catttacgtg aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg ccattgcgca
     7501 cctgaccggc ggcggcttct gggaaaacat tccgcgcgta ttgccagata atactcaggc
     7561 agtgattgat gaatcttcct ggcagtggcc ggaagtgttc aactggctgc aaacggctgg
     7621 taacgttgag cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga tgattatcgc
     7681 cctgcctgct ccggaagtgg acaaagccct cgccctgcta aatgccaacg gtgaaaacgc
     7741 gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta tcgaataatg
     7801 aatattgtgg tgcttatttc cggcaacgga agtaatttac aggcaattat tgacgcctgt
     7861 aaaaccaaca aaattaaagg caccatacgg gcagttttca gcaataaggc cgacgcgttc
     7921 ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc cagcgcgttt
     7981 gacagtcgtg aagcctatga ccgggagttg attcatgaaa tcgacatgta cgcacccgat
     8041 gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc ccactatgcc
     8101 gggcgtttgc tgaacattca cccttctctg ctgccgaaat atcccggatt acacacccat
     8161 cgtcaggcgc tggaaaatgg tgatgaagag cacggtacat cggtgcattt cgtcaccgat
     8221 gaactggacg gtggcccggt tattttacag gcgaaagtcc cggtatttgc tggtgatacg
     8281 gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc actggtgatt
     8341 agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct ggatggtcaa
     8401 cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc ccgtagttaa agcgccagct
     8461 ctgccgctgg cgtttttcaa ttcacctgta aatcgcaagc tccagcagtt tttttccccc
     8521 ttttctggca tagttggaca tctgccaata ttgctcgcca taatatccag gcagtgtccc
     8581 gtgaataaaa cggagtaaaa gtggtaatgg gtcaggaaaa gctatacatc gaaaaagagc
     8641 tcagttggtt atcgttcaat gaacgcgtgc ttcaggaagc ggcggacaaa tctaacccgc
     8701 tgattgaaag gatgcgtttc ctggggatct attccaataa ccttgatgag ttctataaag
     8761 tccgcttcgc tgaactgaag cgacgcatca ttattagcga agaacaaggc tccaactctc
     8821 attcccgcca tttactgggc aaaattcagt cccgggtgct gaaagccgat caggaattcg
     8881 acggcctcta caacgagctg ctgctggaga tggcgcgtaa ccagatcttc ctgattaatg
     8941 aacgccagct ctccgtcaat caacaaaact ggctgcgtca ttattttaag cagtatctgc
     9001 gtcagcacat tacgccgatt ttaatcaatc ctgacactga cttagtgcag ttcctgaaag
     9061 atgattacac ctatctggcg gtggaaatta tccgtggcga taccatccgt tacgcgctgc
     9121 tggagatccc atcagataaa gtgccgcgct ttgtgaattt accgccagaa gcaccgcgtc
     9181 gacgcaagcc gatgattctt ctggataaca ttctgcgtta ctgccttgat gatattttca
     9241 aaggcttctt tgattatgac gcgctgaatg cctattcaat gaagatgacc cgcgatgccg
     9301 aatacgattt agtgcatgag atggaagcca gcctgatgga gttgatgtct tccagtctca
     9361 agcagcgttt aactgctgag ccggtgcgtt ttgtttatca gcgcgatatg cccaatgcgc
     9421 tggttgaagt gttacgcgaa aaactgacta tttcccgcta cgactccatc gtccccggcg
     9481 gtcgttatca taattttaaa gactttatta atttccccaa tgtcggcaaa gccaatctgg
     9541 tgaacaaacc actgccgcgt ttacgccata tttggtttga taaagcccag ttccgcaatg
     9601 gttttgatgc cattcgcgaa cgcgatgtgt tgctctatta tccttatcac acctttgagc
     9661 atgtgctgga actgctgcgt caggcttcgt tcgacccgag cgtactggcg attaaaatta
     9721 acatttaccg tgtggcaaaa gattcacgta tcatcgactc gatgatccac gctgcgcaca
     9781 acggcaagaa agtgaccgtg gtggttgagt tacaggcgcg tttcgacgaa gaagccaaca
     9841 ttcactgggc gaagcgcctg accgaagcag gcgtgcacgt tatcttctct gcgccggggc
     9901 tgaaaattca cgccaaactg ttcctgattt cacgtaaaga aaacggtgaa gtggtccgtt
     9961 acgcacacat cgggaccggg aactttaacg aaaaaaccgc gcgtctttat actgactatt
    10021 cgttgctgac cgcagatgcg cgtatcacca acgaagtacg gcgggtattt aactttattg
    10081 aaaacccata ccgcccggtg acatttgatt atttaatggt gtcaccgcaa aactctcgcc
    10141 gtctgttata tgaaatggta gaccgcgaaa tcgccaacgc gcagcaaggg ctgcccagtg
    10201 gtatcaccct gaagctaaat aaccttgtcg ataaaggcct ggttgatcgt ctgtatgtgg
    10261 cctccagctc cggcgtaccg gttaatctgc tggttcgcgg aatgtgttcg ctgatcccca
    10321 atctggaagg cattagcgac aacattcgtg ccatcagtat tgttgaccgt taccttgaac
    10381 atgaccgggt ttatattttt gaaaatggcg gcgataaaaa ggtctacctt tcttccgccg
    10441 actggatgac gcgcaatatt gattatcgta ttgaagtggc gacaccgctg ctcgatccgc
    10501 gcctgaagca gcgggtgctg gacatcatcg acatattgtt cagcgatacg gtcaaagctc
    10561 gttatatcga taaagaactc agtaatcgct acgttccccg cggcaatcgc cgcaaagtac
    10621 gggcgcagtt ggcgatttat gactacatca aatcactcga acaacctgaa taaccctatg
    10681 ccaatacacg ataaatcccc tcgtccgcag gagtttgctg cggtcgatct tggttcaaac
    10741 agttttcaca tggtcatagc ccgtgtggta gatggtgcca tgcagattat tggccgcctg
    10801 aaacagcggg tgcatctggc ggacggcctg gggccagata atatgttgag tgaagaggca
    10861 atgacgcgcg gtttgaactg tctgtcgctg tttgccgaac ggctacaagg gttttctcct
    10921 gccagcgtct gtatagtcgg cacccatacg ctgcgtcagg cgctgaacgc cactgacttt
    10981 ctgaaacgcg cggaaaaggt cattccctac ccgattgaaa ttatttccgg taatgaagaa
    11041 gcccgtctga tttttatggg cgtggaacat acccaaccgg agaaaggtcg caaactggtt
    11101 attgatattg gcggcggatc tacggaactg gtgattggtg aaaatttcga accgattctc
    11161 gttgaaagcc gccggatggg ttgtgtcagc tttgcccagc tttacttccc tggcggggtc
    11221 atcaataaag agaattttca gcgcgctcgt atggcggcag cacaaaaact ggaaacttta
    11281 acctggcaat tccgtattca gggctggaac gtggcaatgg gcgcttccgg taccataaaa
    11341 gccgcccatg aagtgttaat ggaaatgggc gagaaagacg ggataattac cccggaacgt
    11401 ctggaaaaac tggtaaaaga agttttacgt caccgtaatt tcgcatcgct gagtttaccg
    11461 ggtctttccg aagagcggaa aacagtcttc gttcctggac tggcgatttt atgcggtgtg
    11521 tttgatgctt tagccatccg tgaactgcgc ctttctgacg gggcgttacg cgaaggcgta
    11581 ctgtacgaaa tggaaggacg tttccgtcat caggatgtgc gtagtcgcac cgccagcagc
    11641 ctcgccaacc agtatcacat cgacagcgaa caggcccgac gggtgctgga taccactatg
    11701 caaatgtacg aacagtggcg ggaacagcaa ccgaagctgg cgcatccgca actggaggcg
    11761 ctactgcgat gggccgccat gctgcatgag gtcgggttga atatcaacca cagcggtttg
    11821 catcgccact ccgcttatat tctgcaaaac agtgacttgc cgggttttaa tcaggaacag
    11881 cagctgatga tggcgacact ggtgcgctat caccgtaaag cgattaaact cgacgatctg
    11941 ccgcgcttta ccttgtttaa gaagaaacag ttcctgccac tgattcagct attgcgcctt
    12001 ggcgtattac tcaacaatca acgtcaggca accaccacac cgccaacatt gacactgatt
    12061 actgatgaca gtcactggac actgcgtttc ccgcatgact ggtttagtca gaatgcgttg
    12121 gtactgcttg atctggaaaa ggagcaagaa tactgggaag gcgtggctgg ctggcggttg
    12181 aaaattgaag aagaaagtac acctgaaatc gccgcttaat gctttgcggg ccgacgagaa
    12241 atgtcggccc gcattattca ggcactttcg cgaatgggtt cgatttcatt caacgtatca
    12301 attaacggct gcggcttacc aataagataa ccctgcatat aatcgatccc caaagagagc
    12361 accgcctcgc ggatctcttc gttttcaacg tactctgcca ctacccgcat tttcttcatt
    12421 cgcgccagat ggcaaatcga cgccacaatc tgataatcca gactatttga cacaatattg
    12481 cggataaagc tgccgtcaat tttaagcaga tcggcattca catttttaag ccgtgcatag
    12541 ctggcgtagc cggtgccgaa atcatcaatc gcaatctggc agcctaattc ctgaagatgc
    12601 tgcaaggtga tttgcgcctg cttaacattg gtcagagcat tactttcggt gacttcaaaa
    12661 ataagttgcc acgcttcaat ctgatattta gccagcaact gactgacttc aacaggaaaa
    12721 cgagcctgac ataccgaggt tggagacaga ttaatagcaa aacggtgagc gggcatcttc
    12781 gctctgtttt cagccataaa ttgcagcgta tgctcaatga cccacatgtc gatactcgac
    12841 gataaaccaa attcgtgcgc gaccggcaag aagctatcgg ggacgatcag ttcatcattc
    12901 tcacctttca tgcgcagaag aatttcatgg taaacatcac cacgcatacc ggtaatcggc
    12961 tgggccatca ggaaaaaatg gttgtgttcc agcgcctgct gtagtcgatt catcatcgcg
    13021 actttatctt tcaattcgcg ttgcaaatac attgccccgc gacgctgcat attttccggg
    13081 gcgttggtca cgatggaaag ttcggcgacc gtatttagct ctcccagcag caggtagata
    13141 tgattcactg gcgagcgcac atagcagtaa ctgacgccaa tctgcggttg catcggcatc
    13201 ccatcccaaa agaaacgaaa ttgcttgaga tgactatcca gtgcggtaat gcgctcctgg
    13261 tgcgattctg tattcagtcg cagcgcgaga tcgttacccg aaagctgata aacatcttca
    13321 cccggttcca gcaagggtga cagccagtga gaaagttttt gcttgtattg aatccgcagc
    13381 atgatgccat agttcttaac cagcatttcc atgccaggga tgcgtaaata acaaagcgca
    13441 gaccagggag catcacgcaa cgcgcgattc agggcgcgaa catttggcag atgaaccacc
    13501 gggtccatat acgcaagccg ctgaatgcgt ctgactaccg ctctctgacg ggttgccagt
    13561 acagccatat aattgacaat aaaagagaat accagataac tggaggaggt tatggtcagc
    13621 tgcgtggtat agccaggata aatgggaatg taattttgat agctgtggat gctgatcatc
    13681 aacacgaccg cccagagcag cgaaatcagc ttataaccat agcgcatcgc tccccacatc
    13741 atcaggggca gcaataatga caaggtataa ttggtgctaa aaattgtgct tttttcattt
    13801 aacggcatgc ataacagcaa caataaagca cctaatgcca gtagccagag cgcgaactct
    13861 tttttggtga ctttggcatc aacctgctgt tttaattgcg aataatagct acgtaaataa
    13921 aatggatttc gcactacccg aatgatgaag tagcacagcg ggacaccgat cagattaccc
    13981 accagtaagg cctgataatt gattaaagtc ccgaggttaa aaggcatgac accgaccaga
    14041 ttttctctgc tcgccagtaa tcctacaaat gcagcaaact ggaaaagtat cagaaacagc
    14101 gttgcaggaa acacaatctg ccagaatata cgctgggaaa ttaaacgggt atcaccatgt
    14161 gaaacgttgt tacgccgggg agtaaagacc ctgtaaccgc cccaacagag aatgataatg
    14221 ataataaaat tagccgttaa tgatagcgtt tcgtaaaaac ccagctctgc atatttgcgg
    14281 acaaaaatcc ctaaggcgat ccctggcaat gccgcccagc tgaaaaacat catcatgctg
    14341 atcatcagtg ccagaggcag ataaaagaga aaaacctcac cggaagaaat atgcgcgaaa
    14401 gtattaatgt gggcgaaaat gggcaagatt aaagaaggca ggaacagcgg aagcccccac
    14461 catttatcac gcatttttat ataagttgca ttcagtttca tagatgctca gcagaatccc
    14521 ccacatcctg aaggaggtgt attcagacag gcatcccacc tgacttcgaa tgatgattat
    14581 tcatcactat agagagcatt gattctaagt gtcatatgaa agttccaatt gatatatatc
    14641 aaacaaaata accctgatta atgaattatt acgtttatca tgttaattca tcattattac
    14701 atcatcattg taaataatta aattaacttc cataacatta aaatatgtat ccactgacgt
    14761 ttttttacat aacgaagaat tgaccatttt gtcctgttgt gccttaatgt aagtaccgtc
    14821 cacagcgtgg gacatacttc aaggaacctt ttgtgagtca ggcaaccagt atgcgaaaac
    14881 gacaccgatt taacagtcgc atgacccgta tcgtactgct catcagcttt atcttcttct
    14941 ttggccgttt tatctactcg tccgtcggtg cctggcagca ccatcagagc aaaaaagaag
    15001 ctcagcaatc cacactctcc gtcgaatcac cggtacaacg ttagcggtta ccttcttcac
    15061 tttcacagaa cataacggca cttcgctgtc ggatgctttt gctctttggg attatcaaag
    15121 cggcagatat tctttcatct taaattttac gtctttgtcc tgactgatgt ttatcctgtt
    15181 tggctgcgga ataaatataa aattaatata tatgttgtaa tgatatattt ttataaatta
    15241 ttccctgcgt gaattttaat aaatttaatc tatcccttta tacgcaatac atttactttc
    15301 ctcttttgat gatcttaaat gtcttatttt tcgtaatgtg tataacaagg aatagtgatg
    15361 aaatttaaaa aatgtcttct gcctgtggca atgttagcgt cattcactct ggcaggatgc
    15421 cagtcaaatg ctgacgatca tgccgccgat gtttatcaaa ccgatcaact gaataccaaa
    15481 caagaaacta aaaccgttaa tattatttcc attcttcccg caaaagttgc cgtagacaac
    15541 tcccaaaata aacggaacgc acaagccttc ggcgcgctta ttggcgcagt cgctggcggt
    15601 gttatcggcc acaacgtcgg ttctggcagc aattccggaa cgacggcagg ggcagttggc
    15661 ggcggagctg taggcgcggc agcgggttct atggtgaatg ataaaacctt agtggaaggt
    15721 gtttctttaa catataagga aggcaccaaa gtgtatacct ccacccaggt gggtaaagag
    15781 tgccagttta cgacaggttt agccgttgtt attaccacaa cgtataacga aacgcgtatt
    15841 cagccaaata ctaaatgtcc tgaaaagagc taataatcag gaggagtcat gaagaaagtt
    15901 tttctttgcg ccatcttagc ctccttaagc tatccggcta tcgcctcatc attgcaggat
    15961 caactctctg ctgtcgcaga agcggaacag caaggtaaaa atgaagagca aaggcagcat
    16021 gacgaatggg tcgcggagcg caacagggaa atccagcaag agaagcaacg tcgcgcaaat
    16081 gcccaggccg ccgctaacaa aagagcggca acggcagcgg caaataagaa agctcgtcag
    16141 gataaactgg acgccgaagc cactgcggac aaaaaacgcg atcaaagtta tgaagatgag
    16201 ctacgcagct tagagattca gaaacaaaaa ctgacgctgg cgaaagaaga agcccgcgtt
    16261 aagcgagaaa acgaatttat cgatcaggaa ctgaagcaca aagctgcgca aaccgatgtg
    16321 gtgcaatctg aagctgacgc caacagaaat atgactgaag gcggtcgcga tctgatgaaa
    16381 agcgtgggca aagcagaaga gaacaaatcg gacagctggt ttaattaatc gatgttagta
    16441 acttcaagcc tatgattctt gaagataaaa aaccctctgt agtaacagag ggttttgttc
    16501 attcatagtg cagggattaa aatcattccc actcaatggt agctggcggc ttgccgctga
    16561 tgtcatacac cacgcgggaa ataccgttca cttcattgat aatgcggttg gaaacgcgac
    16621 cgaggaaatc gtacggcaga cgcgcccagt gtgcggtcat aaagtcgatg gtttcgacag
    16681 cacgcagaga gacaacccag tcatacttac gaccatcgcc cattacgcca acggaacgta
    16741 ccggcaggaa cacagtgaac gcctggctga ctttgtcgta caggtccgct ttacgcagtt
    16801 cttcaatgaa gatggcgtca gcacggcgca gcaggtcaca gtactctttc ttcacttcac
    16861 ccagcacacg aacgccaagg cctggtcccg ggaatgggtg acggtacaac atgtcgtacg
    16921 gcaggcccag ctccagacca atcttacgca cttcgtcttt gaacagctct ttcagcggtt
    16981 caaccaggcc catcttcatc tctttcggca ggccgcccac gttgtggtga gatttgatga
    17041 cgtgtgcttt accggttgca gacgccgcag attcgataac gtcagggtag atggtgccct
    17101 gcgccagcca cttcacgtct tccagtttca gcgcttcttc atcgaatact tcaacgaaaa
    17161 cgcgaccgat gattttacgt tttgcttccg gatcgttttc gccagccagc gctgacagga
    17221 agcgatcttc tgccggaacg tgaacaatgt tcagaccaaa gtgatcgcca aacatatcca
    17281 gaacctgctc tgcttcgttg agacgcagca ggccgttgtc gacgaatacg caagtcaggt
    17341 ttttaccgat agcgcggtgc agtagcattg cggttacgga ggaatccacg ccaccagaga
    17401 ggccgaggat gactttatcg tcgcctacct gctcacggat gcgggctaca gcatcgtcga
    17461 taattttcgc tggcgtccac agggcttcac actggcagat atcacgcaca aaacgctcca
    17521 gcatgcgcat accctggcgg gtgtgagtca cttccgggtg gaactgtacg ccatagaagc
    17581 gtttttcttc gttagccata atggcaaacg ggcagctttc ggtgctggct acggtgacga
    17641 agtcggacgg gatagcggta actttatcgc cgtggctcat ccagacatcg agcagcggtt
    17701 taccgtctgc ggtcagcgca tcttcgatac cgcgaaccag tgcactgtcg tttacgactt
    17761 caacctgcgc gtagccaaat tcacgttcgt tagaggcttc aacgtgaccg cccaactgca
    17821 ttgccatggt ctgcatgcca tagcaaacgc cgaataccgg tacgcctgct tcaaagacat
    17881 actgcggcgc acgcgggctg ttttcttcag tagtactttc cgggccgccg gaaagaataa
    17941 tgccgcttgg attgaagtca cgaatttgtg cttctgtcac atcccacgcc cacagttcgc
    18001 agtaaacacc cagctcacgc acgcggcgcg caaccagttg agtgtactga gaaccgaagt
    18061 ctagaatgag gatgcgatgc ttatgaatgt tttccgtcat tgacgcttat tccgaggcaa
    18121 gtgaaacaga taatataaat cgcccgacat gaagtcgggc gaagagaatc aggagcccag
    18181 acggtagttc ggggactctt tagtaatggt cacgtcgtga acgtggcttt cctgaatgcc
    18241 cgcaccgctg atacgtacaa actccgcttt agtacgcagt tcgtcgatag taccacagcc
    18301 ggtcagaccc atacaggagc gcaggccgcc catctgctgg tgaatgatct ctttcaggcg
    18361 acctttataa gctacgcgac cttcgatacc ttccggcacc agtttgtcgg cagcgttatc
    18421 gctctggaaa taacggtcag aggaaccttt ggacatcgcg cccagggaac ccataccacg
    18481 gtaagatttg taagaacggc cctggtagag ttcgatttca cccggagatt cttcagtacc
    18541 cgccagcatg gaacctacca tcaccgcgct tgcgccagcg gcgatagctt tggcgatgtc
    18601 gccggagaag cgaataccac catctgcgat aaccggaata ccggtgcctt ccagggcttc
    18661 aactgcgtca gcaacggcag tgatctgcgg aacacctacg ccagtaacga tacgagtcgt
    18721 acagatagaa ccagggccga taccgacttt aaccgcgctg caaccagctt ctgccaaagc
    18781 gcgtgcacct gcagctgttg ccacgttgcc gccgatgatt tgcagatccg gatatttagc
    18841 acgggtttca cggatacgct gcaaaacgcc ttctgagtga ccgtgggagg agtcgatcag
    18901 cagaacgtca acgccagcgg caaccagcgc gtcaacacgc tcttcgttac ccgcacctgc
    18961 gccaactgca gcaccaacac gcagacggcc ttgctcgtct ttacaggcgt tcggtttacg
    19021 ttccgctttc tggaagtctt tcacggtgat catgccgatc aggtggaatt cgtcatcaac
    19081 caccagcgct ttttcaacgc gtttttcgtg catttttgcc agcaccactt cacgggcttc
    19141 accttcacgc acggtgacca gacgctcttt cggcgtcatg taaacgctaa ccggctggtt
    19201 caggtcggta acaaaacgca cgtcacgacc ggtgataata cccaccagtt cgttttcttc
    19261 ggtaacgacc ggatagcccg caaaaccgtt acgctcggtc agttctttca cttcgcgcag
    19321 cgtcgtggtt ggcagaacag tctgcggatc agtcaccaca ccagattcgt gttttttcac
    19381 acggcgaact tcttctgcct ggcgttcaat ggacatgttt ttgtggataa agccgatacc
    19441 gccttcctga gccagagcaa tagccaggcg cgcttccgtt acggtatcca ttgctgcgga
    19501 aagcataggg atattcagac gaatagtttt cgtcagctgg gtgctgaggt cagcagtatt
    19561 cggcagaacg gtagaatgag caggaacgag gagaacgtcg tcaaacgtca gagcttcttt
    19621 agcgatacgt agcatgggca atatctcgac cagagtggtt aataaatatt gccgcggcat
    19681 tatacagagc gtaaccgatt gcatctaccc ctttttgcaa aaaatgcttg ctatccccga
    19741 agggcgggtt actatcgact gaataacctg ctgatttaga atttgatctc gctcacatgt
    19801 taccttctca atcccctgca atttttaccg ttagtcgcct gaatcaaacg gttcgtctgc
    19861 tgcttgagca tgagatggga caggtttgga tcagcggcga aatctctaat ttcacacaac
    19921 cggcttccgg tcactggtac tttacactca aagacgacac cgcccaggta cgctgcgcga
    19981 tgttccgcaa cagcaaccgc cgggtgacct tccgtccaca gcatggacaa caagttttag
    20041 ttcgcgccaa tattacgctc tacgagccgc gcggtgacta ccagataatc gttgagagta
    20101 tgcagccggc cggtgaaggg ctgctgcaac agaagtacga acagctcaaa gcgaagttgc
    20161 aggctgaagg tttgttcgat cagcaataca aaaaaccact tccctcccct gcgcattgcg
    20221 ttggtgtgat cacctcaaaa accggtgctg cgctacatga tattttgcat gtgttaaaac
    20281 gtcgcgatcc ttctctaccg gtgatcatct accccaccgc cgttcagggc gatgacgcac
    20341 cggggcaaat tgttcgcgcc attgaactgg cgaatcagcg caatgagtgc gacgtgttga
    20401 tcgttgggcg cggcggcggt tcgctggaag atttatggag ttttaacgac gaacgcgtag
    20461 cgcgggcgat ttttgccagc cgcattccgg tcgtcagcgc cgtcgggcat gagacggatg
    20521 tgaccattgc cgattttgtt gccgatctgc gtgcgccaac gccgtctgcc gccgctgaag
    20581 tagtgagccg taatcagcaa gagttactgc gccaggtgca atcgacccgt caacggctgg
    20641 agatggcgat ggattattat ctcgccaacc gcacgcgtcg ctttacgcag atccatcacc
    20701 gattacagca acagcatccg cagctccggc tggcacgcca gcaaaccatg cttgagcgcc
    20761 tgcaaaagcg gatgagcttt gcgctggaaa atcaacttaa gcgtaccggg caacagcagc
    20821 agcggttaac acagcggctg aatcagcaaa atccacagcc gaagattcat cgcgcgcaaa
    20881 cgcgcattca gcaactggaa tatcgtttag cagaaaccct gcgcgtacag cttagcgcca
    20941 cgcgtgaacg tttcggtaat gcagtaacgc acctcgaagc cgtaagccca ctgtcaacgc
    21001 tggcgcgtgg atacagcgtt actactgcta ctgacggcaa tgtactgaaa aaagtgaagc
    21061 aagttaaagc gggtgaaatg ctaaccacac gtctggaaga cggctggata gaaagtgaag
    21121 ttaaaaacat ccagccagta aaaaaatcgc gtaaaaaggt gcattaagcc agcacgaatt
    21181 caacgcgttt tttcgatatc aagccatgcc cgtgctgaca gaagtaatct actgcaccgc
    21241 aggcttttaa cacctgcaac ggttgatggc agtctggaca aagagctttc atttcaataa
    21301 tttccccgca ggatgggcaa cgcgcatggc cgttatcctg atcaagcaca tgctgacatt
    21361 gtggacaatg cagttccata tgaattcctc cggtagccat ccctctacat tcatagaggg
    21421 aatggcagat aaaatactta cggataatta tttatttttc ttgatgtgct tcatcagacg
    21481 cttacgttta cgcatctggg ttggcgtcag ggtgttacgc ttgttcgcat acgggttttc
    21541 cccttctttg aactgaatac gaatcggcga tcccattacg tccagcgatt tgcggaagta
    21601 gttcatcaag tagcgcttgt aggaatcagg caggtctttc acctgattac cgtgaatcac
    21661 cacaatcggc gggttataac caccagcgtg ggcatatttc agcttcacac gacgaccgcg
    21721 taccagcggc ggttggtgat cttcaacagc catcgtcatg atgcgcgtca gcatagaggt
    21781 ccccacacga cgggtggagc tgtcatacgc ttcacgtact gattcaaaca agttaccaac
    21841 accactgccg tgcaaggcag agataaagtg cacacgagca aaatcgataa agcccagacg
    21901 gaagtccagc gtttctttca cctgctcttt cacttcctgg ctcaggccat cccacttatt
    21961 caccacaatg acaagtgagc gcccactatt gagaataaag cccagcagcg agagatcctg
    22021 atcggaaata ccttcgcgcg catcaatcac taacatcacc acgttggtat cttcaattgc
    22081 ctgcaacgtt ttgattacgg agaatttctc tacagcatcg gtgattttgc cgcgtttacg
    22141 tacgccagcg gtgtcaatga gcacatactc acgtccatcg cgttccattg gaatgtagat
    22201 actgtcacgc gtcgtgccag gcatgtcata aacaacaacg cgctcttcac caagaatacg
    22261 gttagtgagt gtagacttac ctacgttcgg acgacccaca atcgccagtt tgatcggcag
    22321 actttgcggg tcgaagtcgt cttcctcttc ttcttcgccg ttctcttccg cttcaaattg
    22381 cgcccagtat tcagcgtctt cgtcgacttc ctcttgcggt gcgagatctt ccatccacgg
    22441 cagcagcaca cgctccagca gacttaatac gccacgaccg tgagatgcgg cgatcgggta
    22501 gatttcacct aaaccaagcg agtagaaatc aaccactgcc tgatcgggat ccagaccgtc
    22561 agttttgttt gccaccagga aggtcggttt ttcacgagag cgcagatgtt tggcaatcgc
    22621 ttcatctgcc ggcatcaagc ccgcgcgcgc atccaccata aacagtacga cgtccgcttc
    22681 ttcaatcgcc agcagcgact gttccgccat gcgggtttct acaccgtctt ctgtgccatc
    22741 aatcccgccg gtatcaatac agataaactc acggccttca atttccgcac gaccgtactt
    22801 acggtcacga gtcagacccg ggaaatccgc aaccagcgca tctcgggtgc gagttagacg
    22861 gttaaataac gtggattttc ctacgttagg gcgcccgaca agcgcgacca caggtaccat
    22921 gtttaaagcc tcatttttat aaatcatcag acaacgcacg ctatattcgc gtcgttgtta
    22981 aaaacaggaa aacggcccct gtccaggagc cgttttcaaa gtgaacgaca gagacgatta
    23041 acgtgtaata gagtacacgg ttccgtcttt tgcctggatc agcagtttgc cgtcagcggc
    23101 aaccggttca gtctggaaac cggaactatc aactttttgc tgggcaacga aacgaccatc
    23161 ttcgacgtta atccagtgca gataaccttc actgtcacct accaccaggt tgccattata
    23221 cagcaccggc gaagtcagca ggcgatgcag cagatcgctt tgtgtccaca gcgtaacgcc
    23281 gccatcaatg gtcaacgcca tcacccggtc attttgatcg accagataga tgcgattgcc
    23341 gtcgacgatg aaatcattca ccgaacccag ttcgcgtttc cacataatct gaccactgcg
    23401 cagatcaagc gccgtcaggt taccattata ggccagagcg aaaacaacgc cgttaacaac
    23461 gacgggagtc gtgtcaacat cgctcagacg gtcaatttcg gtagaaccgg tcgcctggga
    23521 aatacgctgc tgccaaatca tctggccctg ttccatcagc actgcgctga cgcgaccatt
    23581 atcgcccccc acgacggccg caccaaaagc cgttgccggc gcagactcgc cacgcaaaga
    23641 gagcgaaggc atatcgaggt taactgtcca tttgacagcg ccgtcagctt cgttcagcgc
    23701 ttgtaactga ccgttactag tgtggattaa caccagaccg tcgctgacca ccgggcgaga
    23761 aagtgcttca cccgcgactt tagtttgcca tgccacagta ccatcgctgg tattcagcgc
    23821 gtaaacctgc gccttttcgc tgccaatgta gacatgccca ccagacacgg tcacaccgcc
    23881 agaaagtaat gcaggctctt tagagaacca gccatctttc tcggccaggc tgacagacca
    23941 gatttctttg ccatcatccg cattcagcgc ttttaccaaa ccagcgcggt ccgctgcata
    24001 gacaacgttg tccgccagtg ccggatgaag attggaatag aagttgccaa tgccgctacc
    24061 aacggaagtg ctccacgccg tggtcggcgt aaactggttt tcaacggttg gcaatgggga
    24121 catctttacc acatcttctt cgctgttaaa cagcgaacag ccgcttaaaa gggtaacgga
    24181 aagcagtcct ggcagcagta atttacgcaa ttgcatcggg tccctctcag atggacaaat
    24241 tattaatttt catctgcatc atttcgctca gtgccggagt aacatcgctt ttcacgcctg
    24301 cttcccatgc actacgcgca ccttgcttat cacctttgct caacaatgct ttaccacgca
    24361 ggtcggcaac aatggcagcc cacccttcac ctttgatggt atcaagggtt ttcagcgcgg
    24421 catcagcctg cttgagctgt acctgaacgc gagcaagacg cagatttatc acggctttga
    24481 gattttcatc actcgtgtct gccagcccct gttgtaactg ggcggcagct ttctctagtt
    24541 catttttgtc aacaaattgc tgcgcaagtt ccaaagaagc cagcgcacca taagtatttt
    24601 tattttcagc agcaaatttt tccgccgccg ggatgctatc cggtttgcct tcgctcactg
    24661 cggtaaccgc attttgatag gcaagagaag cggagcgtgc agaatcaacc tgatggctgt
    24721 tccagtagcg ccagccaatc agtgcgccaa cgcccaaaat caccccaaca gccagtgctt
    24781 tgccattttc agcaaaaaag cgtttaaccg cttctacctg gtcgttttcg ttctcgtaaa
    24841 tttccacgct gtccttctcc ttccttaacc cagtaacgtg cgcaaatgcg cggctacgct
    24901 atcctgcgca actgccgttt gctcaccaga gcgcaaatcc ttcactactg ctgtgccgtt
    24961 agccacttca gactcaccca gcaccacagc aacgcgggca ccccatttat cagcacgggc
    25021 aaactgtttc ttaaagttgc cgccgccgtg gttggtcatc aatttcacgc ccggtaattc
    25081 atcacgcaga cgctcagcta atgccatagc cgcagattgt gtatcagcac ctgaagccac
    25141 caggtatata tcgacaacag gatcggcttt aaattccgga ttaacggcct gtactaacaa
    25201 tacaagacgt tcgaggccca tcgcaaaacc gactgccggt gttgcacgac cgcccagttg
    25261 ttccaccaga ccgtcataac gaccgcctgc acacacggtg ccctgggagc cgaggctgtt
    25321 agtcacccac tcgaaaacgg tacggttata gtaatccaga ccacgcacca gacgctggtt
    25381 tacggtgtaa gcgatccccg cgctctcaag cagtttgcac agaccggcaa aatgctcacg
    25441 agattcctcg tccagataat cacctaatgc cggagcgtcg ttgagaagcg cctgcacttc
    25501 cggatttttt gaatccagca cgcgcagcgg gttagtgtac atgcggcgtt tgcagtcttc
    25561 gtccagcttt tctttatgct gctcaaggaa tgccaccagc gcatcgcggt aattggcgcg
    25621 tgcttccagc gaaccaatag agttcagctc aagagttacg tgttcgaaga tacccagcgc
    25681 acgccaccag cgggcagtga gcataatcag ttcagcgtcg atatccggac cttgcagacc
    25741 gaaaacttcg cagcccaact gatggaactg acgataacgc cctttctgcg gacgctcgtg
    25801 acggaacatc ggcccgatat accacagacg ctgttcctga ttgtacagaa gaccatgctc
    25861 gatgccggcg cgtacacagc ccgccgtccc ttcagggcgc agagtcaggc tgtcgccatt
    25921 gcgatcctca aaggtgtaca tctctttttc aaccacgtcg gtgacttcac caatcgcacg
    25981 tttgaatagc ggggtctgct ctacaatcgg caagcggatt tcactgtaac cgtagctgcc
    26041 gagcacgttt ttcagtgtgc cttcaatgcg ctgccagatg gccgtttcgc caggcaggta
    26101 atcgttcatg ccgcgaatgg cttgaatgtt ttttgccacg tttattctct ttctgaatat
    26161 aaaaatgaac cctcaacgct tccctcaatg tttcgggagc catgcgggtt caatcataca
    26221 cgggaagcga ggcgcttccc atcacgttat tatttttcaa cctgctgaac gtcaattcga
    26281 cgcgcttcgt ccagctgact ggctttcgca cgaatgcgtg cttccagctg gtcgatcata
    26341 tcgttgttgt ccagacggtc tttgcgcacg ccatcttcat agaggccgct tttcttgttg
    26401 ccgccggtga cgccgagtgt agaaaccagc gcctcacctg ggccattcac cacgcagccg
    26461 ataatcgaaa cgtccatcgg agtgatgata tcttccaggc gttgctccag cgcgttaacc
    26521 gtaccgataa catcaaattc ctgacgcgaa caggtcgggc aggcgatgaa gttaatccct
    26581 cgcgaacgga tacgcagcga tttcaaaata tcgaaaccga ctttgatctc ttcgaccgga
    26641 tcggccgcca gcgatacgcg cagcgtgtcg ccgatgcctt ccgacagcag cagacctaaa
    26701 ccaatggcgg attttactgc cccgctgcgt gcaccaccgg cttcggtgat ccccagatgc
    26761 agcggctgat cgatctgttt tgccagcaaa cgataagact caacagcgag gaagacgtca
    26821 gacgctttca cgctgacttt gaactgatcg aagttcaggc gatcgagatg atcaacatga
    26881 cgcatggcgg attccagcaa cgcctgcggc gtcggttcgc catacttttc ttgcagatct
    26941 ttttccagcg atccggcgtt aacgccaata cggatcggaa tgtttttatc gcgcgcacag
    27001 tcaaccacca tgcgaatacg ctcttcatta ccgatattgc cagggttaat acgcagacaa
    27061 tcgacgccgt attccgctac tttcagcgca atgcgatagt cgaagtggat gtcagccacc
    27121 agcggcacgt taacccgctg tttgatgagt ttgaacgctt ctgccgcgtc catcgtcggt
    27181 acggagacac ggacgatatc agcgccaacg cgttccagcg ccttgatttg attgaccgtt
    27241 gcttcgacat ctgtcgtacg cgtattggtc atggactgta cggcgatggg agcaccatcg
    27301 ccaatcggca cattcccaac gtaaatacgt gttgattttc tacgttgaat tggagcctgg
    27361 ttatgcatga aaaatctccc gcgttacccg tctgttactg cgccggtgat tgttcggcat
    27421 tgagggtcag acgcgcaacc tggttagttc tgataaaacg actcagatcg acaggtttcc
    27481 cttgatactg gatctgtact gcggctggcg caccaatttt cagtttatac ggtgcctggc
    27541 cggttaaatt caaattaccg tctttacgct gcatcccgct aaacaatttt ttaccggtag
    27601 catcagtgac ctccagccag caatcggcag taaagttcat caccagcgca ttcggatcag
    27661 ccgccggcgt ggtcacgcca gcctgatcgg ttggcaacgg cgcagcacca tctggcgttg
    27721 ttgctgccgt tggtaccggg gtcgcggcgg tatcaacatt tgcctgcgaa ggcgaaacaa
    27781 ccgcattctg ttgcggatca acagctggtg ctggcgcagt tacggcaggt gtttgcgtgt
    27841 tggttgcggt agtatccaca gacgctggcg gcgtggacgt tgtagccggg tctgtagttg
    27901 tcgacgtatt taacgggaca ctctgcccct gctcgctatt actgctcagt tctgccgaag
    27961 attgatcggc catagtggtg atctcttcct gctgagcttt gtggtcttgc caccaccagg
    28021 caccgctcag gccgataacc acaaacaaca ccagccaggt gaaggtcatc agccagccat
    28081 cacgtttttt gcggcgttta ccgagggaaa aactctgcat tggcgcaact tttgcagccc
    28141 gtagtggagc ctgcttttcc agccctggca gcagttcttc ttctggaata tgtaccagac
    28201 gcgcataaga gcggatatat ccgcgcagga atgttgaagc aagatcggcg ggtgccttat
    28261 cttcttcaat gtcgcgtacc gtggaaacct tcaggcaaag tcgctcggca acggcctgct
    28321 gactaagtcc tagttgttcg cgagcattac gcaggcgagc gccggtagta agtgcttcat
    28381 tttggtcgtg cgtggcttca gtattcattc gctgcaggta cgtttaaatg agaattgaga
    28441 tgccggtgaa ccatcatgct cacccacacc gcgaaacatc cgttaagtta accgttatca
    28501 tacagtataa gaccgtctgt gtgctcgtga caaagcccac attcactaag gctaacttac
    28561 tgttgcatcg ttacatactg ccttaaagtc agcaaaaacg caccgttaat attgaccaga
    28621 caattgcaac ttaatacttc attcataaaa cattacgcca cggtacataa agtaaccgtg
    28681 gcgtaatggc tatcagaccg ctttaatgtc gatggcttca ccctgcatcc gtttacgcag
    28741 ggtacgtttc gtacggtcga taacatcgcc cgccaactga ccacaggcag catcgatatc
    28801 atcaccacga gttttacgca caatagtggt gaaaccgtag ctcatcagca cttttgagaa
    28861 acggtcgata cggctgttcg agctgcgtcc atacggcgca cccgggaacg ggttccacgg
    28921 gatcaggttg atcttacacg gcgtatcttt cagcagttcc gccagttggt gcgcgtgttc
    28981 agtgccgtcg ttaacgtggt caagcatcac gtattcaata gtgactcggc cctgattggc
    29041 gttggatttc tccagataac ggcgcaccgc agcaaggaac gtttcgatat tgtacttttt
    29101 gttgatcggc acaatttcgt cacgaatttc gtcgttcggc gcgtgcaggg aaattgccag
    29161 tgcaacgtcg atcatatcgc ccagtttatc cagcgccgga actacaccgg aagtagaaag
    29221 cgtgacgcga cgtttagaca ggccaaaacc gaaatcatca agcatgattt ccatcgccgg
    29281 aacgacgttg ttcaggttga gcagcggctc gcccatgccc atcatcacta cgttagtgat
    29341 tggccgctga ccggtgactt ttgctgcgcc gacgattttc gccgcccgcc acacctggcc
    29401 gataatttcc gacacccgca ggttgcggtt aaagccctgc tgggcggtgg aacagaattt
    29461 acactccagc gcacacccca cctgcgaaga gacgcagagc gtggcacggt cgtcttccgg
    29521 gatatacacc gtttcgacgc gctgatcgcc aacggcgatc gcccatttaa tggtgccgtc
    29581 ggatgaacgc tgttcttcaa ccacttccgg tgcgcggatc tccgccacct ctttcagttt
    29641 gccgcgcaac actttgttga tgtcggtcat ctcatcaaag ttgtcgcagc aatagtgata
    29701 catccacttc atcacctgat cggcgcggaa gggtttttca cctaaatctt taaaaaactc
    29761 ccgcatctgc tgacggttga gatccagcag gttgattttt ccatctttcg tggtgacgtt
    29821 ttcaggtgtg actaattgtt cagacatatg ctattccggc ctcgttatta cacgttatgg
    29881 cccctggagg gttgaaaaaa gaaacgcccc ggtgagcggc tgctcgtccg ggggcgctgc
    29941 attgtacaaa ttctggcgca cggatgccac gtttgcacgc ggcatttacg aaattattaa
    30001 cgggtgcgcg ggcacacttc gccttcgccg aagaaataag cgatttcgcg agcggcagat
    30061 tcgacggaat cagaaccgtg ggtaccgttt tcggtcaggc tgtcagcgta atcagcgcgc
    30121 agagtaccgg ccagtgcgtt tgccggattg gtcgcgccca gcagatcgcg gtgacgctga
    30181 acggcgtttt caccttccag cacggaaacc acgatcgggc cagaggtcat gaattcaacc
    30241 agaccatcaa agaacggttt tccatcgtgt tcagcataaa agccacgagc ctgttcaacg
    30301 gtcaggtgca gcattttggt gccgacaatt ttgaaccctg cagcttcaaa gcgcgcaaag
    30361 atattaccaa tgacgttttt tgctaccgcg ttcggtttga tgatggaaaa agtacgttca
    30421 atagccattt ttacctctgt aaattgttct gttgttgtct gtaccagcgt acgagatggc
    30481 gcggattata atgagcaaca gggccgttga ctattgatga aggtaacatt tttttaaaat
    30541 aaaatgagtt ttagcaacaa tcagggacaa aacagactat tgcatgacaa atttcactgt
    30601 cgcgatttgc cccacgtcat ccatcaccag caattgataa tcacctttat ccgtcaaatg
    30661 cagggtcacg ttgcgcccgc gttcagttaa cggttcgcca ttcagaaacc accagcgttc
    30721 acctgcccct ccacttgatt gcaacggcaa agtcgcttcc gctgcgcccg gtaaacgttt
    30781 aataatcgcg ccatcgcgaa cgcctgtcag ctgcagaggg agctgggcat cgtgaccgta
    30841 tggcgggcaa attgtcgagg ccggtggtaa gcgcacagcg cgacgttctg atgcgggcag
    30901 ccagggttcc agcggtagcg gccagacatt taccatttct tgcttcgcct gcgggcagtc
    30961 agcggcgacg cgctgccctt tgtcatccag ccagatgggg aagcgaatgc cattaatccc
    31021 ttcctgctct gacagtaaca gcgttggcgg ctggctgcca tccagcagcc aggttgccag
    31081 gcggcggcga cagttaccgt caccttccgg caaagactgt ccgcccggcc agcagataac
    31141 gccacgagtg actgagttcg ggcgcgggtc ttccggcaga ttcgcactgc gcgacagtaa
    31201 gatattattg acctgattca acaatggtac ggcactggca aagccaaact gaccaacaac
    31261 gggcgtgccg tcaggtctgc cagtccagat cccaatgaca tagcgagcgt taaccccaat
    31321 cgcccaggcg tcacgatagc catagctggt gcccgttttc catgccagtg gggcgacgcg
    31381 cggcaaggca ctatccggca agggttgcgc ttcatcagcc ataatccggc gaatgatcca
    31441 cgccgccccc gacgacatta aaggccgttc aagcagcgga tcgtcaggct gtaagcgcaa
    31501 tttgcctgcc ttgccgtggc gagcaaacgc ggtatacgct gccgccatat cttccagttt
    31561 tgcaccagcg ccgccgagaa tgagtgaaag attcggcgca gcgccgttgg gcaaatataa
    31621 cggcaatcca acattgcgta actttgccgc aaaccgtttc ggtccatagg cttccagcac
    31681 ctgcacagca ggtaagttca gcgagcgcac cagcgcctcg ctcatgctga tcgggccatg
    31741 aaaaccgcta tcaaagttac ctggtcgata atcaccggtg cgccggggga cgtcttgcag
    31801 cagtgatgcc gggtggatca agccttcatc cagcgccaga ccataaacaa acggtttgag
    31861 caccgatcct ggcgatcgga tcgcattgac catatcaaca tgaccaaagc gtgaatcatc
    31921 gttgagatca accgatccca cccagccgcg aacacgcata tcggtatgat caaccacgat
    31981 catcgccagt gagctgcgcg gtggcaaccg ccctttccag ttttgcgcca gttcttccag
    32041 acgtcgttga agaccggcat ccagcgtagt aacgattttg tcgcttttgc ttttaccgag
    32101 catcatgcgc gaaaacagcg gtgccagttg cggcatttgt cggggtgcca gccagatggg
    32161 ttcttccctt gactctttta cctgctcacg ggaccacaca ccttgcgcag ccatccgttc
    32221 gagcacttta ttacgcgcgg cttcggcacg ctccggccaa cgatccgggc gaagacggct
    32281 gggcgcttgc ggcaaaaccg ccagcattgc cgcctcggaa tagcttaaat tcgcaggcga
    32341 ctttccgaga taagcccaac ttgccgcacc gatcccctgc aacgtaccgc caaacggagc
    32401 gcggttaaga tacaaggtca gaatttcacg cttagacaga tgccattcca gttgcaacgc
    32461 gcgccagagc tggcgaattt tgccgccaaa tgttttgggg tgaggatcaa gcagacgagc
    32521 aacctgcata gtgagcgtgc tgccaccgga aataacccgt cccgaagtga gatcttgcca
    32581 tgctgcgcgc gccaccgaga atggattcac ccccggatgc ttccagaacc agcgatcttc
    32641 ataattgatc agcgcttcaa ggtaacgtgg agaaacatct tcgattgtta ccggataacg
    32701 ccagatgccg tcagcatcgg cgaagcgcca gagcggcgta ccatcctgcg ccacgaccac
    32761 tcgtgcggga ttgacttcat gcagcggtag aggccagagt ttatctgctc cccacgctgc
    32821 aagaaaaagg agaaatggcg cagctgccag cgttatccag cagccgcgtt tggttaacaa
    32881 gcgaggcatt tacggtcgga caatcagcag atcttcagcc gcgccggtcg cccgccattg
    32941 gggaacatac attgattcca ccatcggttg cggtacctga tacgttcccg gcgtcaccgc
    33001 ccgcgccaga tacaccagcg ttaccggttg gtattcatca acggcaaccg ccgccacaaa
    33061 gcgatcgtca cggaactcaa tgtgcttaat actcgcctgc tgcatctggt tcagtaagtt
    33121 ctgcacttcg ccaccacttt gctccaggct ggcgctaccg ttcgccagat tctggttttc
    33181 cagttccaga cccacaggca gcagatccac gactaacgca tccggcacgc tgttactggc
    33241 ttttacctgc aaccacacca gcaccagatc gccgctacgt aacgagtcca gcgatttgct
    33301 cttaccatca gtaccaagaa tatgacgctc gatttgcagc acattgttcg ccggtaaagg
    33361 tgcggattgc ggataaccgc tggcatccat acgcaaccat aacggctgat cgccactgtt
    33421 gctcacctgc aaggtgacaa gttgatcgct attcagattg ctgttttgcg ctttctcgcc
    33481 tgtcagcggc tcagctgaga aagaggtttg cgcctgccat ttacccggta aatcctgaat
    33541 cgtacgggca gccaggaaca acgcgttact ttcctgcgtc gatagccagc gttcaccaaa
    33601 cgcctgctgc gaaagtgtgt tcagcaaagt gtactgctca tcgggtagca gtttattttc
    33661 ttccagcaag gagagcatta acgcgttgtc gcgcagtgga ctaccgtaat cacccagcca
    33721 tatccgctca tcactatttc tcggcgtttt cagcgccagc gcaatcgctt cttcaccacg
    33781 cgtcgcatca cccatggttt tcagcgcaat gccaagttgc agcagcggta aaccagaagc
    33841 ggcatctgca cgatgctccc agatttcacg cagcgcaccc agtggtgctt tttgctgacg
    33901 ggccagcacc agcgcagcgt aagactgaac ggcgaattta ctggctttga gattatccgc
    33961 gtacgggatc gacatcatgc ccggatcttg taaatagcgc agcagacgct cattaccccg
    34021 gttaatggcg tctgtcggca cgctgtaacc ctgttcgcct gcgcggacca ggaaatccat
    34081 cacgtaagcc gtcagccagt actcttcgtc accgttttta tcccacagcg caaagccgcc
    34141 gttatcacgt tgcatttgca gcaaacggga aatgccgata tcgaccgatg cacggcgttt
    34201 ctcatcactg tcgcctttga tgcccaacgc ctgcagttgg gctgcgttgg tataaagtga
    34261 cgggaacagg ccgctggcgg tttgctcaag acagccgtac ggatacgctt ttaactcttt
    34321 gatataacgt gcaatgttca gcggtggttt gccgctcaac aacaattgcc cttccagcgt
    34381 aacaggcgag aagttttgca atccatccgc cggaatcgcc catgtctcac caggctgtaa
    34441 cgccgtaccg taattaaccg tttgtgccgg gaacgccgga cggacgccga ttttccactg
    34501 cttatgctga tcggcaacgg tttcacccgg taacgctaac ccgctaatgg tggcctgaat
    34561 ttctccatcg ccataacccg gcaatgctcg caccgggata aacagcgtag tacgcacacc
    34621 tggcgctaat tcaacggctg cgggtgaatc gctgaccagt tcaagcaaac cactggcggt
    34681 cagggcaacg ttcagttttt gcggtttatc ggtaagatta gtgatatcca gcgtcagacg
    34741 cgaggtatcg ccactcgcca taaagcgcgg catgttcagt tcagcaatca ccggtgcggc
    34801 aactatcact ttgctttcgt tgctaccgaa atcatctgcc gtccaggctt gcgccatgac
    34861 gcgcaattca ccgttaaaat cgccaatcgg cagtgtaacc gagccttcgc cctgttcgtt
    34921 gagcgttacc gacagcgcct gctgcgcgac aatattgacg tgattgaccg gcggtttacc
    34981 accacgtttc agctcatcac catcgccacc gaaacgcaga gctgccagac gcccctgacc
    35041 ttcaataacc tgaccgtaaa tatcgtaaat gtctgcgcca tagcgtttct gaccaaagaa
    35101 cgcctgccac ggatctggcg taacgtagtc agtaatattc agcacaccac tatcaacggc
    35161 agacaccagc acattcacct gtttaggctt ctcgccattt ttagtgctgg ctttaatttt
    35221 cacggttaat ggttgattgg gacgcatttt tgctggtgtt tccagcgcca gatcgaggcg
    35281 acggttttca tcaccaagcg gcagatgcaa cacaccaacc gcgcgttttg gcgtcgcgga
    35341 gcgagattta tcgccaggac gcaccaccag cgtactcaaa taaagatcat gacgattcca
    35401 ggttttatcg accggaatcg tcagatccag cccttgagcc ggaacatcaa tctcttgcca
    35461 ccacagcggc ccttcactgg actcgaccat cgcataacct ttacccgccg ttggcgcggc
    35521 aatatgcaac ttaatggtat cgccggggcg ataactggct ttatctagtt tcagcgtgac
    35581 acggtcgggt cgcactgcgc cgctcccgtc gctgttgtcc tgccagctat agccagccca
    35641 aaaacgcacg ctactgaccg cttcattcgg cgctttgact tccagacgat aagcgcccca
    35701 ctctaccggg aaactgacct taccggtttc atccgcctga agatccagag tttgttcatt
    35761 ttcgatcaga tctttttgat caaactgtga ctgccagcct tcatcttctg accagttcca
    35821 gtagtaatcg cggcgttcgc gaatcaggcg cacctgcaag cccgacacgg cttttttcac
    35881 gccttgcgca tcgctataaa cgatgtcaaa agcggcgtta ctgccttcat caacaatggg
    35941 ctgtttcacc gtgctgtcag tgcgataatc gtaaaccgat ttcaaggcga actgcggacg
    36001 gagccccggc aatgcatcgg caggccagat agcctgctca gcgcggcgcg tcaccgggcg
    36061 accgcccgat tccagcaaac tgccctggaa aataacctgc aatggggaat gcgtttcctt
    36121 ccactggctt tctgtagaaa cttcgccgcg ccctttatca tccagcgtca actgaacttc
    36181 atccagcgtg cgggaaaggt tttcggcagc tatatcgccg aattcaaaac caggtaaggc
    36241 tgacacagcc tcacgcagtg ggcgcaggaa aagttgccct tgcaaagtat taccattagc
    36301 aggcgcaccg tacaggtagt atcccaccac ggagaatttc acttcatcat tcggcgtaag
    36361 cggggttttc tcaccggtca gattcagcgc catacgctct ggcataaaat cttcgacgtg
    36421 gaaatcccac atccggtact ggttatcgcc cgtgttggcg cgaatatgcc acataccggt
    36481 tgccgcattg ctatcgagtg gccaggtaaa gtggtagagg ccattctccg gctgactaac
    36541 gacgttcctg agtacctgcc cgtcaggttt aatcacgtct aacttgatgg gttgatcggg
    36601 caatgcttta ccgtctgcat cgcgcagtaa accattgagg attaccgttt cacctgggcg
    36661 ataaagatcg cgtgggccaa acatgaaaaa ctgtttgcta tagcctggcg cgccagcaat
    36721 gttaaattct gctaagtcca gcgccggaag ttttaaatcg agtagcgttg tctgaccgtc
    36781 tttacgtgcc agcagtaatg ccgcattttt atcattttcc agctgcacat gcccctgagc
    36841 gtcactggtt gcctgagtca gagtctgtcc tttctcattt aataaagaga cttcaattcc
    36901 ttgctgggcc gcgccgtttt ccagactttg ggtaaagata tccagacaat tgtgataacg
    36961 gtgagctgaa acgccgatat cacttaacgt aaacagcgtc gcgggattac tgtaatcgta
    37021 acgtccagcc tgattcatca cagccagata cacgcccgcc tgctgaagcg gtttgatatc
    37081 gcccagcggc agcaataatt tttcacgggt gttacgcgca ggattgagat caaaccgtcc
    37141 ggtgtagacc agatccgcca tctgcagcag tttgtctgac tgccagttcg ccagcgaatt
    37201 gcggtattcc cattggctaa tgaatgctgg cagagattct ggcttaacgc ggaagaagtt
    37261 aacatcgaca ttattaacgt tgagcgccat taccggcagc ccttcaacga ctttgccagg
    37321 cagcagcgaa ccacggctgg caaaaccgac gctgggttgg atgtcgcggg tagttatagt
    37381 tttttcgtaa tctttactga aggttgcgtt gttgagtgct ttgacctcct tgccaatagt
    37441 aacgatcaaa tcacgtttcg gttcgaggtg gcgtaaacgt agctctttaa gattatctga
    37501 cagctcccag gcaccatcca ctttgccgct ttttttatcg acgacatgaa taacgcgtga
    37561 gaaatcctga tccgggtcga gagggatgga gaacgtcagc accagcgtgg ctgcaccatc
    37621 aagttggact tcagagagat ccagcaatgt cagcacctta ccggcactct gttgggccag
    37681 tttttgtctc tccggcacgg agagttttgc tgaactcgcg ttttctgaag aggccgcttt
    37741 agtaacttca gaaggcgcat cttttttcac cgctgttggc gcgttatcgt tgttgtcgca
    37801 ccctgccagc gccagcatta gcatgcaggc ggctacgcgt aactttttca tttttcatcc
    37861 ctgccacaat ggcccgttag caacgtcgaa taattattat gcgtgagaat ttacgttatg
    37921 taattcagta tcacgcccat acaatccgaa ttttagtgag aattgtggct cagacagcca
    37981 aatcagcctc tttcgcttgt cgcttttcac taaacatccg acaatttagc ctaaccccgg
    38041 caaaaatgga gatgcctatg tccacgacat ggtttgtagg agccgactgg ctcgccgaac
    38101 atattgatga cccggaaatt cagattatcg atgcccgcat ggcgtcgcct ggacaggaag
    38161 atcgtaacgt tgctcaggag tatctgaatg gacatattcc cggcgcagtg ttttttgata
    38221 tcgaagcgct ttctgatcac acttccccgc ttccgcacat gctgccgcgc ccggaaacgt
    38281 tcgccgtggc gatgcgtgaa ttaggcgtta accaggataa gcacctgatt gtctatgacg
    38341 aaggtaatct tttctcagcc ccacgagcat ggtggatgct gcgcaccttt ggtgtagaga
    38401 aagtgtcgat tctggggggt ggacttgcag gctggcagcg cgatgatctg ctgttagaag
    38461 aaggtgcagt agagctgccg gaaggagagt ttaacgccgc gtttaatcct gaagccgtgg
    38521 tgaaagtaac cgatgtatta ttggcaagcc atgaaaatac ggcgcaaatt attgatgccc
    38581 gcccggctgc acgttttaac gcagaagttg atgaacctcg cccaggttta cgtcgcggac
    38641 atattcccgg agcactgaat gttccgtgga cggaactggt gcgcgaaggc gaactaaaaa
    38701 cgaccgatga actggatgcg atattttttg gtcgcggcgt cagctacgac aaaccaatta
    38761 tcgtcagctg cggctctggt gtaacggcag ccgtggtttt gttagcactc gcgacgctgg
    38821 atgtgccaaa cgtgaaactg tacgacggcg catggagtga atggggcgcg cgggcagatt
    38881 taccggttga gccagtgaaa taagtatttt acaggcaata aaaaaccgcc gaatttggcg
    38941 gttttttatt gctggtctgg ttcgcggcct ttccagcagg ttgtattacc gtagtaatgc
    39001 aagcgcgtct cagcggagac aatactcgcc agtaactctc tttttgtcaa gcaaaagaga
    39061 gttattattg ttctgttagt gtattatcca ctgcggccct ttccgccgtc tcgcaagcgg
    39121 gcgctggctt aaggaaagga tgttccgcga ccgttagagc aagcgcgtct cagcgcttgc
    39181 tatcgcggca atatcgccag tggtgctgtc gtgatgcggt cttcgcatgg accgcacaat
    39241 gaagatacgg tgcttctgta tcgtacttat tgtttctggt gcgctgtttt cccaagtaaa
    39301 taataaccgg agtctctccg gcgacaattt actggtggtt aacaaccttc agagcagcaa
    39361 gtaagcccga atgccgccct ttagggcggc atattttaga ttatccggtt ttgtttaaag
    39421 tcacgcaaaa aaccacccca gcgacgttca tagaatggca caatatgttc ggtaataaag
    39481 tggctaattc ctttttcccc ttttttcacc tgacaaatat cgattggttc atcgccaggt
    39541 aatgtatcgg tcgctacact tcccgtcgcc tgaataattt cttcgatatc accatcggct
    39601 tcaatgccaa taagtaaatt aggctgtgcc tcttcgttct ctttaattga acaaataaaa
    39661 gcacgcttca ccggcttaat ggttttaaat aaggtggtga gtgaatcaat catttgtgct
    39721 ggcggctctg cgacttccga taatatcagc gattcaccgc cttccaggat ttcctggctg
    39781 ctcagcggat ttccctcttc accaatcaac aaactgattt cacgcggcat aaattcttta
    39841 ccggtcggca gtttggcatt aaggaagagc gtttcgccaa gggtcatctc aaacaatgtg
    39901 cgaacgggca ttacgacaaa tgcctgttcg tcttcaaccg cctgttgaag cgcttctaac
    39961 gaggtgaaaa aaggaatgac gctggtgccg tcttcttttt cccagtgctg taaatcaagc
    40021 gcactatctt caaccacagc ctcgccctgc gccgccgtac caggcaccca gacggtggat
    40081 tccagtagag tacggaaaaa ggccgggcgg tgcgccggtt cggttgctgc tttttccagc
    40141 aggtcttcaa gttcgttttt tgtttcggac ataagaacca caattcattc aacgttcggg
    40201 gcgcaaatgc ctgatgcgct acgcttatca ggcctacaag ggattcgcaa tttgttgaat
    40261 ttgcagaatt tgtaggccgg ataaggcgtt tacgccgcat ccggcatatt agtttacgcc
    40321 gttaacagat tagctatcgt gcgcacacca agtcccgtag cgcccgcaga ccactgttca
    40381 accggcgctt tacggtaagt cgccgagcag tcgatatgca gccagccttg ctgatagttc
    40441 tcaacaaagt gcgacaggaa gcccgccgcc gtgctcgcgc ctgccggata cgccgcgctt
    40501 ccggtattgt tcagttcggc aaagttagac ggcagctggc tgcggtggaa ttccgccagc
    40561 ggcagacgcc agaatggttc gttctcttgt gaggcactcg ccagcaaacg accggcaagc
    40621 gcatcgtcaa aactgaacag cgcgtgataa tcattaccca gcgcagtttt cgccgccccg
    40681 gtgagggtcg ccgcatcaat gatcatttcc ggtttctgcg cactggcatc aatcagacca
    40741 tcggcaagca ccagacgccc ttcggcatca gtgttcatca cttcaacttt tttaccgttg
    40801 cgataggtga tgatatcgcc cagcttgaac gcattgccgc taatcaggtt atccgcacag
    40861 cagaggaaca gcttcacgcg cttgttcagt ccgcgcgtaa tggcaaatgc cagcgccccg
    40921 gtaaccgttg ccgcgccgcc catgtccgac ttcatcgagt ccataaacgc agtctgtttg
    40981 atgctgtagc cgccggagtc aaaagtgata cctttaccta ccaggcacgc gtacactggc
    41041 gcttctttat cgccagttgg gttgtaatcc agcgccagca ataccggaga acgttctgaa
    41101 ccgcgtccga cggtgtgcag ccccatataa ccttgctcac gcagatcttc gcctttggtg
    41161 atccgataag tcacacgatc gcccgcgacg ttgctgatca gatcaacagc acgctgtgcc
    41221 agttgcgatg gtcccaattc ttctgccggt gcgttgatgg tgtcacgcac ccagtcgatg
    41281 atcatcaggc ggttatccag ttcctggcgc tgggcatcgt ccagatccgg ccactccact
    41341 ttacgcgtgc ctttcggggc tttgtaacct tgccagaatg cccagcagcg atccgcatcc
    41401 cagccttcac cgcttaactg aacatgcttg atgcccagac cgtcaatctt gcgcgccgca
    41461 cgctggatca gccccagatc gtctgccccg ttcaaatgca gggtaatgcc gtcattatta
    41521 atgctgtaag ttgctttttc tccccagcgc gcgtcggcag gttgggtaga gagggtaatc
    41581 ttcatcgctt ctgtcatttt agttatcctt ctttgtaaaa gggccgccag ttggcagccc
    41641 caggattttt ccgctaaatg attcgcgttg cagaaaggcg gcaagtgagt gaagccccag
    41701 gagcatagat aactatgtga ctggggtgaa cgagcgcagc caacgcatct gcggcgtgaa
    41761 gcatgacgcg gaaattattc ggcctcgtcc agccagacta acaaaatcgc ttcgagaatt
    41821 ttctcgttgg atgcctgcgg gtcgtcgtcg aaatcttcca gatcgcaaat ccactgatgc
    41881 atatcggtga atcgaaccgt tttcggatca agatcaggat acgcatcgta cagtgcttcg
    41941 ccaatttcgc ggctatcggt ccacttaagt cccatactaa cctctgttaa tgctcacgcg
    42001 catggttgat agtgtaacgc gggatttcga ctactaaatc ttcgtcggta acacgtgcct
    42061 ggcagcttaa acggctttcg ggctccagtc cccaggcttt gtccagcatg tcgtcttcct
    42121 gctctgagct ttctggcagc gagtcaaaac cttcacgaac gatgcagtgg caggtggtgc
    42181 aagcacagga tttttcacag gcgtgttcaa tctcgatacc gttacgcaga gctgcgtcga
    42241 gaatggtttc accgctatta gcttccagaa cagcgccatc aggacagaga tcctgatgag
    42301 gcaaaataac aatctttggc atattaaacc tcgtccacgg aatggccttt cagcgcacga
    42361 cgaaccgact ggtccatgcg gcgagcggcg aaatcctggg tttgtttgtc tacgttttta
    42421 atcgcttgtt cgatggcgtc aacgtcatcg ccctgcgcca cttcactcag gtgagcggca
    42481 gcatcgtcaa tgacctgacg ttctgcggcg cttaacagcg cggcatcagc agccagcgcg
    42541 ccgtgcagac tttccagcac acgcgccgct tctacttttt gttctgccag cattcgggct
    42601 tttacgtcct gctcggcata gctcattgag tctttgatca tcgaagcgat ttcgctatcg
    42661 gtcagaccgt aagacggttt gacctgaata gacgcctcaa cgccggtgga tttctccatc
    42721 gccgtcacgc tcaaaagacc gtcggcatcg acctggaacg tcacgcgaat atgcgcacca
    42781 ccagccggta gcgccggaat accacgcagc gcaaaacgcg ccagtgagcg gcagtcctgc
    42841 accagttcgc gctcaccctg cattacatgg atagacatcg ccgtctgacc atctttaaag
    42901 gtggtgaaat cctgagcgcg ggccaccgga atagtggtat tacgcggaat cactttctcc
    42961 accaggccgc ccatcgtttc gaggcccagc gacagtggga tcacatcaag cagcagcatt
    43021 tcgctgtctg gcttgttacc caccagaata tccgcctgaa tcgccgcgcc aatagcgacg
    43081 actttatccg ggtcgatgga agtcagcggt ggacgaccga aaaattcgcc tacccgttca
    43141 cgcaccagcg gcacgcgagt agaaccgccc accatcacca cttccagcac ttcgtcagct
    43201 tctacacccg cgtctttaag cgcgcgacga caagccagta aggttcgttt taccagtggc
    43261 gcgatcagtt cattgaattg ttcacggctg atttcgccct gccagcccgc aacgttaacg
    43321 gtcacggagt ccgcatcgct cagcgcgatt ttggctgcaa tggcggcatc cagcagttca
    43381 cgctgaacgc ggttatcgct acgatcagga atgcccgcct gctcgcgaat gtaatccgcc
    43441 agcagatggt cgaaatcatc gccgccgagc gcggaatcac cgccggttgc cagcacttca
    43501 aacacgccgc gacttaagcg cagaatggaa atatcaaacg tcccgccacc gaggtcataa
    43561 acagcgatca cgccttcctg accggaatcc agcccgtagg cgatagccgc agcggtcggt
    43621 tcgttaagta agcgcagaac atgcaacccc gccagacgcg ccgcgtcttt ggtgccctga
    43681 cgctgggcat cgtcaaagta cgccggaacg gtgataacta caccatccag ctcgcctgcc
    43741 agggcttcag ttgcccgcgc cgccagtgct ttgaggatgt ccgcagaaac gcgcaccggg
    43801 ttcagcagcc ccgccgccgt ttcaatcatc ggcaggccgt tttcgctggc ctggaattga
    43861 taaggcagat gcggatagcg ttgctggata tcagccagcg agcgccccat caggcgttta
    43921 acagaactaa tggtgttggc ggtgtcaagc gctgcgttag tacgcgcgtc ataacccacc
    43981 gaatgccctt gctgttgata gtgaacaaca gatggcagca ggtgacggcc ttcatggtcg
    44041 gctaacgttt cggcctgacc gctgcgcact gtcgccacca gcgagttggt tgtgcccagg
    44101 tcaataccag ccgccagacg acgctgatgc ggcgcagcac tcaaaccagg ttcactaatt
    44161 tgtaataagg ccatgtttag cttccagaaa ttaaaaatcg agcagttttt cttcgagttg
    44221 ttcggcactg ctccgcagtt tatcgagaaa acgcagctta cgcacggtat ccgccgccgc
    44281 gtcccacgcc tcgttgtcta actgttcaac catcaactga tggcgggtat caaacatctt
    44341 tttcacacgt ttgataaagc tttccagccg cgcttcatct ttcgcctgtt cgatctcgtc
    44401 cagctcttcg cgcagctcca actgttccat caggaacgcg gtgtcgcgca cagtatgctg
    44461 ctcgctggcg agatcaaaac cgtgtaaaga aagcaaatat tccgcgcgca ttaacggatg
    44521 acgcagcgtt tgccaggcct ggttaatggt tgcagattgc tgtacggcgg cgagttgttc
    44581 cgcctggctt ccgctggcga atttatcagg atgatactga cgttgtagat cctgaaaacg
    44641 caggctcagc gcctgggtat cgagttgata acgggcaggc aagccaaaga aggtgaagta
    44701 atccataaca ttctcagggc tgcgatccgc cgcgataacg gcgaatcagg taaaacaaac
    44761 cccacgcgca ggcgaccacg gtggggttat cggtatgcgc atcaaacgtg gaagctttcg
    44821 ccgcaaccac actcatcttt gacgttcggg ttggtgaatt taaacccttc gttcaggcct
    44881 tcttttacga agtccagctg cgtaccgtcc agaaattgca ggcttttgcc atcgaccacg
    44941 actttcacgc ctttgtcttc aaacacgatg tcttccggcg tcggttcgtc aacaaattcc
    45001 agtacataag ccatacctga acatccggag gttctcacgc ccagacgcag gccaaaccct
    45061 ttgccgcggt tagccagaaa ggtatttact cgcgctgctg cactgtcgct cagtgtaatc
    45121 gacataacca aacctcaact cttattttgc ttcacgtttg cttttatagt ccgcaatggc
    45181 ggctttgatc gcgtcttctg ccagaataga acagtgaatt ttcaccggcg gcagttcaag
    45241 ttcttcagca atatcggtat ttttgatcgc ctgcgcttcg tcgagagact tccctttcac
    45301 ccattcggtg accagggagc tggaagcgat agcagaaccg cagccgtaag ttttaaaacg
    45361 cgcgtcttca atgatacctt catcgttgac tttaatctgc aacttcatca cgtcgccaca
    45421 ggccggtgcc cccaccatgc cgctgccgac gttctcgtcg ttgttgtcaa aggaacccac
    45481 gttacgcgga ttctcgtaat ggtcgataac tttttcgctg taagccatta taaattctcc
    45541 tgattccgat accgattaat gatgagccca ttcgatgctg ttcagatcca cgccctgctt
    45601 gtacatttcc cacagcggag aaaggtcacg cagacgaccg atggatttac gaactaactc
    45661 gatggtgtag tcgatctctt cttcagtagt aaaacgacct aaagagaaac ggatagagct
    45721 atgtgccagc tcgtcgttca gccccagcgc gcgcagcacg taggacggtt cgaggcttgc
    45781 tgacgtacag gcggaacctg aagaaactgc gaggtctttc agcgccataa tcagcgactc
    45841 accttcaacg tagttgaagc tgacgttgag aatgttcggc gcaccgtgtt ccaggtcacc
    45901 gttcaggtaa acttcttcga tatctttgat gccgttccac agacggttac gcaggccgcg
    45961 cagacgttcc atctcggtcg ccatctcttc ttttgcgatg cgataggcct cgcccattcc
    46021 gacgatctgg tgaacaggca gagtgccgga acgcataccg cgctcgtgac cgccgccgtg
    46081 catttgcgct tcgatgcgta cgcgcggttt acgacgtaca tacagcgcac cgataccttt
    46141 cgggccatag attttgtgac cggagaaaga catcaggtca actttcaact ggctcaggtc
    46201 gataggcagt ttgcccacgc tctgggttgc atcaacgtga tagataatgc cacgagcacg
    46261 gcacatttcg ccgatagccg cgatatcctg caccacgccg atttcgttat ttacgtgcat
    46321 gatggaaaca aggatggtgt catcacgcat cgctgcttca agttctttca gatcgataat
    46381 gccgttacgc tgcggtgcca ggtaggtgac ttcaaaacct tcgcgctcca gctgacggca
    46441 ggtatccagt accgctttgt gttcggtttt gctggtgatg atgtgcttgc cttttttctg
    46501 ataaaagttg gctgcacctt tgatcgccag gttgtcagat tcggttgcac cagaggtaaa
    46561 gacgatttca cgcggatcag cgccgaccag atcggcaatc tgattacggg cgatatctac
    46621 cgcttcttca gcctgccagc cgaaacggtg agaacgggag gccgggttac caaaggttcc
    46681 gtccatcgtc ataaactgca tcattttctc ggcaacacgc gggtccaccg gcgtggttgc
    46741 ggagtagtcg agataaatcg gtaatttcat tgctctataa actccgtaca tcactcaatg
    46801 caaggaacca ggctaccggc tggatgtacg accgtgttta cggagcgcga tacgccccgg
    46861 cctgattcta atatcttgtt atctttaacc taactgtttc gggtttcagc aagttgacaa
    46921 acttacgaat ctccgggagc ttacataagt aagtgaccgg agtgagtatg tgcagtcaac
    46981 gcagatgcaa cacgaaacag gacggttaaa ttaagcgcgt aacttaacgt cgatcgcgtc
    47041 ttgtatgcgg gtgcgtggcg cgtcgtgagt atgctgacga ccagacacat ccagcacttc
    47101 ctggttatta accagttcgc ctaaagtaat gttgttgaga aaaccggtga gacggtcgct
    47161 caaatcacgc cacagcgcgt gggtcaggca tttatcgccg ccctggcaac cgcctttacc
    47221 ctgacaacgg gtagcatcta cagattcgtc aacggcgcta atcacttcgc caacggcgat
    47281 gctgctggca tctttgccta acagataacc accgcctggt ccacgtacgc tggaaaccag
    47341 accattttta cgcagacggg aaaacagttg ttccagataa gaaagggaaa ttccctgacg
    47401 ttcggaaata tcagccaacg gtaccgggcc cgcttcagag ttgagcgcaa cgtcaagcat
    47461 tgcggtcacg gcatagcgcc ctttagatgt cagtctcatg tcttacttca cctcaaactc
    47521 gcccctgccc ggggtttttt attgtaaaag tgggggtatt gcatagcagg gtcaagtctg
    47581 acattcccga gtaaattggt caactattta cttgactgat ttagtcgggt atttaacctt
    47641 cagtgccatt tttttatcgt ggcgtttgta cctgttgtcc ttccagacca tgggcgggtt
    47701 gagggcaatt attcgcatta gcgctggcct cttcccctca ccctaaccct ctccccgaag
    47761 gggcgagggg accgtccgtg catttatctg gcttagccct caggccttat tacctttatt
    47821 ctgctgctct atagaagcca gaatcccgcg caggatattc aattcctggc tttccgggcg
    47881 cgcacgggta aacagacggc gcaatttatt catcacctgc cccggatggt tttcacggat
    47941 aaaaccggtt gccagcagcg tttgttccag atgaccataa aaacgctcca gatcatcgac
    48001 cagcggatac ggcgtctctt catgctcgac ctgctcgcca ttttcctgag ttgccagcca
    48061 ggccatacgc acttcatagg caataacctg aaccgccatt gccaagttca gcgaactgta
    48121 ttccgggttt gccgcaatcg cgacatgata atggcatttc tgcaactctt cattggtcaa
    48181 gccgacgcgc tcacgaccaa acaccagcgc caccggggta tttgccgctt cagcgacgct
    48241 tttcagaccg cattcgcgcg ggtcgagcat cggccacggc agcgtgcggg agcgtgcgct
    48301 ggtgcccacc accaggctac aaccggctaa cgcttcgtcg agcgtatcga cgatgtgagc
    48361 attaccaatc acatcgctgg cccctgctgc cagggcaatc gcctgggagt cgggtttcac
    48421 cagtggatta accagccaca gattggttaa tcccattgtt ttcatggcac gggcaacaga
    48481 acccatattg ccggtgtgtg acgtctccac cagcacaatt cgaatatttt gcagcattgt
    48541 ctttcttcat ctaaagatta ttcacgcatc ttatcataaa acgaagacag atgccgatct
    48601 cgctgctata ctctgcgccg ttttcccgtt ctttaacatc cagtgagaga gaccgatgca
    48661 tccgatgctg aacatcgccg tgcgcgcagc gcgcaaggcg ggtaatttaa ttgccaaaaa
    48721 ctatgaaacc ccggacgctg tagaagcgag ccagaaaggc agtaacgatt tcgtgaccaa
    48781 cgtagataaa gctgccgaag cggtgattat cgacacgatt cgtaaatctt actcacagca
    48841 caccatcatc accgaagaaa gcggtgaact tgaaggtact gatcaggatg ttcaatgggt
    48901 tatcgatcca ctggatggca ctaccaactt tatcaaacgt ctgccgcact tcgcggtatc
    48961 tatcgccgtt cgtatcaaag gccgcaccga agttgctgtg gtatacgatc ctatgcgtaa
    49021 cgaactgttc accgccactc gcggtcaggg cgcacagctg aacggctacc gtctgcgcgg
    49081 tagcaccgct cgcgatctcg acggtaccat tctggcgacc ggcttcccgt tcaaagcaaa
    49141 acagtacgcc actacctaca tcaacatcgt cggcaaactg ttcaacgaat gtgcagactt
    49201 ccgtcgtacc ggttctgcgg cgctggatct ggcttacgtc gctgcgggtc gtgttgacgg
    49261 tttctttgaa atcggtctgc gtccgtggga tttcgcggca ggcgagctgc tggttcgtga
    49321 agcaggcggc atcgtcagcg acttcaccgg tggtcataac tacatgctga ccggtaacat
    49381 cgttgctggt aacccgcgcg ttgtgaaagc catgctggcg aacatgcgtg acgagttaag
    49441 cgacgctctg aagcgttaat gactcaggcg ggtgatgtca ctcacccgcc ctcgcctttc
    49501 aggcgctatt ctgaaatact tcctcaccgc tttactttct ttcgtcactc tcccaccatc
    49561 tttccccgat taatggatga taaagaagtc acaaggaaag gttatggcgc tgccagtgaa
    49621 caaacgcgtt ctcaaaattc tgtttattct ctttgttgtt gccttctgcg tttatttagt
    49681 gccgcgcgtt gccatcaact tcttctatta tcctgacgat aaaatttacg gtcccgaccc
    49741 ctggtcggcg gaatccgtcg aatttacggc taaggacggt actcgtctgc aaggctggtt
    49801 tattccttct tcgacgggcc ctgctgacaa tgccatcgca accatcattc atgctcacgg
    49861 caatgccgga aatatgtccg cccactggcc gctggtcagt tggttacccg agcgtaattt
    49921 caacgttttt atgtttgatt atcgcgggtt tggtaaatca aaaggcacgc cgtcccaggc
    49981 cggattgctg gacgatacgc aaagtgccat caatgtggtg cgccatcgca gtgatgtaaa
    50041 cccacaacgg ctggtgctgt tcgggcagag tattggcggg gcgaatattc ttgctgttat
    50101 tggtcaggat gatcgtgaag gcatacgtgc ggtgatcctc gactccacat ttgcctctta
    50161 tgcaaccatc gccaatcaga tgatccccgg cagtggctac ttacttgatg agagttacag
    50221 cggcgaaaat tatatcgcca gcgtcagccc gatcccgctt ttactcattc acggtaaagc
    50281 tgatcacgtt atcccatggc agcacagcga aaagttgtat agcctggcaa aagagacaaa
    50341 acggttgatc ctaatcccgg atggcgaaca cattgatgct ttttccgatc gtcacggcga
    50401 tgtttatcgc gaacagatgg tggactttat ccttagcgcg ttgaatccgc agaactaacc
    50461 catgatcgct agcacgataa tcattcacaa aaccacctta agacatgcta atccactggt
    50521 cagaacagtt taagatgaga aaaattctgt gacgcttgcc aacatttctg atgattagca
    50581 ttcccttcgc catttccctg agcaaacttt agctattctt atcaattatg cttatgggag
    50641 atctacagat gatgcctacg cttgctccac catctgtcct ttcggctccc cagcgccgct
    50701 gccagatctt gctgacgctc tttcagccgg ggttgaccgc caccatggca accttcagcg
    50761 agcttaatgg tgtggatgat gatattgcca gtcttgatat cagcgaaaca ggacgggaga
    50821 tcctgcgcta tcatcaactc acactgacga ctggttatga cggtagctac cgggttgaag
    50881 gtacagtgct taaccaacgt ttgtgtttat ttcactggct acgacgtggt ttccgtctgt
    50941 gtccgtcatt tattaccagc caattcaccc ccgccctgaa gagtgaactg aagcggcgcg
    51001 gaattgcgcg taacttttac gacgatacca atctacaagc gttagtgaat ctctgctccc
    51061 gacggctgca aaaacgcttt gaaacgcgcg atattcattt cctgtgtctg tatctgcaat
    51121 attgtttgct gcaacaccac gctggaatta cgcctcagtt taatccgctc caacgtcgct
    51181 gggcggaatc ctgccttgaa tttcaggtag cgcaggaaat tggacgccac tggcagcgtc
    51241 gggcgctcca gcctgtaccg cctgatgagc cactgtttat ggcattactt ttttccatgt
    51301 tgcgggttcc cgatccattg cgtgatgcgc atcagcgaga cagacaattg cgtcagtcta
    51361 tcaaacgtct ggtaaaccat tttcgtgagc tgggaaatgt tcgtttttat gatgaacagg
    51421 ggttatgcga tcagctttat acccacctcg cccaggcgtt aaatcgcagt ttgtttgcca
    51481 tcggtattga taataccctg ccggaagagt tcgccagact gtacccacgc ctggtgcgca
    51541 ccacccgcgc ggcgctggcc ggatttgaaa gtgaatacgg cgtccatctt tctgatgagg
    51601 aaagcggtct ggtcgcggtg attttcggtg cctggctaat gcaggaaaac gatctgcatg
    51661 aaaaacagat tattctactg accgggaatg atagcgagcg agaagcgcag attgagcagc
    51721 agttacgcga actaacgtta ctgccgctca acattaagca tatgtcggta aaggcatttt
    51781 tgcagacagg cgctccgcgt ggcgcggcac tgattattgc gccttatacc atgccattac
    51841 cgctcttttc accaccgctg atctatacgg acctgacgct gacaacacat caacaggagc
    51901 agatccgcaa aatgctcgaa tcagcatgag ggaacaactt tcgggcgcaa aaacattgcc
    51961 ggaagcgcca ccagcgccat tacccagaac acgccgtggc ccagatattg atacaggaaa
    52021 ccggcgaaaa cggtcatgat agcgatactg ccgcccatcg cgacggcaga gtaaaccgcc
    52081 tgtaaacgga tgacttcgct accctggcga acagcaatat agcgcatggc ggccaggtgg
    52141 cagaccgtga aggtgccgca atgcaaaatt tgcaccacta tcaaccacgg caacgccgta
    52201 gttgctccca taatgcccca gcgcactacg ccgcaaatcg ccgagatcaa cagcatatcg
    52261 cgagcactac aacggcggaa aagtttatta ctcagcgcaa agataatgac ttccgccacc
    52321 acgcccagcg accacaaata ccccaccgcc gaggccgagt agccagctgc ctgccagtaa
    52381 atggcgctaa aaccgtaata ggccgcatgt gccccctgca ataaacaaac gcaggccaga
    52441 aagcgccagt tctggcgaac cagcgccaac cacgcagacc aaccggtgct ctcctgctgg
    52501 cgacttgccc cttgtggctg aatcgtcgga cggatgagaa agccgagcag catggatgcc
    52561 acgcccaacg tcaacagcgc gaggatcacc cgataatcaa acatagtgac cagtttgccc
    52621 gtcagcgccg agccaatgac aaacgccacc gagccccaca gtcgcacttt gccgtaatca
    52681 agcgggaact gcttttgcca cgtattcgcc agtgcatcgg tcaacggtac cagcggtgag
    52741 aaaaagaggt taaagccaat catcaccagc atcagccacg ctacgtgcgc ccccgcccag
    52801 aaggcgacag caaagagaag tgtcagcagt gccagcacgc gcaaggcgga aatcaggcgg
    52861 gaaggatcgc tgacgcgggg cgcgatgagc aaactcccga gaaaacgggc aaccagacct
    52921 gcccccaata acaggccgat ggtttctggc gttaaaccaa tccctttaag ccagacgctc
    52981 cagaaaggta gaaaaatgcc gtaactaaaa aagtatgtga aatagccgag cgccaaccag
    53041 cgcgtggatt gcaaaaccat gagtccctcc cgtcaggagg cgttagtctg gggttaatcg
    53101 aatgttggtg caagttgaaa aataagaata ttcgagcctg cttcccgatg tctgtaacgc
    53161 cagttcacaa gcactaaaaa actgcgatcc gcgacgcatg ttgcaaaaat aatgcgtaat
    53221 tatgccgtta cgcttgccaa acgttcctgc acaatggcga tgaaatcgcg taatgccggc
    53281 ttcatctccc ctttcttcca cgccatcagt aaagcaatgg aaggcacatt tccggcaatg
    53341 ggacgaaaaa caacctgccc ggtattaaaa ttattcatat aaccgggtat caaagtgacg
    53401 cccagcccca tgcccaccag attcatggtc accagaatat tcgttgccac ctggacgata
    53461 tttggctggc tgttttcttg cgcaaaccac gctttaacga tcggcgcaag cgaaccggaa
    53521 tacgccggat cggtactgac gaaattcacg ccatccagtt gcgcggcggt gatctctttt
    53581 tcatgtgcta aagggtgatc aaccggtaac accaccacta atggttcgtc aaaaagctcc
    53641 agataatcaa tctccgggct ataaacagga tggcgcatta agccgacgtc gagttcacca
    53701 cggcgaattt tttcctcctg ttgcgtggtg attaaactca ccagctcaat caaggtgtct
    53761 ggctgtctga gacgaaacat tggtaatact tttggcaata aattcacttc cgccgatggc
    53821 acaaagccaa tggttaattg tctgtcttcc tgaacaattt tccgcgcccg taatttggca
    53881 ttttccgctt gttcgaggat tgccagcgcg tcctggagaa aacattctcc cgccgccgtc
    53941 aacgcgactt tgcgcttatc cctcaccagt aacggaacac cgacacagtt ttcaagatcg
    54001 cggatctggc tgcttaacga aggctgtgag gtatgcagtt tttccgccgc acgggtaaag
    54061 ttcagtgcct gcgccactgc gacgaaatag cgtaaatgcc gtagttccat caccttcccc
    54121 ttgttatcga aaaaacgtct cagctggtag aaattaacta tttcacatat tagcaaccaa
    54181 ccaacaacat ccttatgaca caaaaataga aggtcaatac atcttatctt tcaggattaa
    54241 aaaatatgac cacaccctca gatttaaaca tttaccaact gattgatacc caaaatggtc
    54301 gggtcactcc gcgtatttat accgacccag atatttacca actggagctt gaacgtattt
    54361 tcggccgttg ctggttattt ctcgcccacg aaagccagat cccaaaacct ggtgatttct
    54421 ttaacaccta catgggggaa gatgcggttg tcgtagtgcg tcagaaagac ggcagcatta
    54481 aggcgtttct caaccaatgc cgccaccggg ccatgcgtgt gagttatgca gattgcggca
    54541 acactcgcgc ctttacctgt ccgtatcacg gctggtctta tggcattaac ggcgagttga
    54601 tcgatgtacc gctggaacct cgcgcctacc cacaagggtt gtgtaaatcc cactggggac
    54661 taaacgaagt tccttgtgtg gagagttata aagggctgat ttttggcaac tgggatacca
    54721 gcgcaccggg cctgcgtgat tacctgggtg acattgcctg gtatctggat ggcatgctgg
    54781 atcgtcgcga aggcggcacc gaaattgtcg gcggcgtaca aaagtgggtg atcaactgta
    54841 actggaaatt cccggcagag cagttcgcca gtgaccagta tcatgctctg ttcagccatg
    54901 cttctgccgt tcaggtatta ggggcgaaag atgatggcag cgataagcgc ctcggtgatg
    54961 gacaaaccgc ccgcccggtg tgggaaaccg ccaaagatgc gctgcaattt ggtcaggacg
    55021 gtcacggtag cggtttcttc tttactgaaa aaccggatgc taatgtctgg gtcgatggcg
    55081 cagtttcaag ctattaccgc gaaacctatg ccgaagcaga acaacgttta ggtgaagttc
    55141 gcgccctgcg cctggcgggt cataacaata ttttccccac gctttcatgg ctcaacggca
    55201 ctgccacgct ccgcgtctgg catccgcgcg gccctgatca agttgaagtg tgggcgttct
    55261 gtattactga caaagccgcc tccgatgaag ttaaagccgc ttttgaaaac agcgccactc
    55321 gtgcttttgg tcctgctggt tttctcgagc aggatgactc ggagaactgg tgtgaaatcc
    55381 agaaattgct taaaggccac cgcgcccgca acagcaaact gtgtctggaa atggggcttg
    55441 gtcaggaaaa gcgccgcgac gacggcattc ctggcattac taactatatc ttttcagaaa
    55501 ctgccgctcg tggaatgtac caacgctggg ccgatcttct gagtagcgaa agctggcagg
    55561 aagtgctcga taaaaccgcc gcttaccagc aggaggtgat gaaatgagtg cgcaagtttc
    55621 actagagtta catcaccgca ttagccagtt tctctttcac gaagccagct tactggacga
    55681 ctggaaattt cgtgactggc tggagcagct cgacaaagag attcgttaca ccatgcgcac
    55741 cacagttaac gcgcaaacac gcgaccgccg caaaggcgtc cagccaccga caacctggat
    55801 ttttaatgac accaaagacc agctggagcg gcgaatcgcc cgtctggaaa cgggcatggc
    55861 ctgggcagaa gagccgccgt cacgcacccg tcacttaatc agcaactgcc agataagcga
    55921 aaccgacatc ccaaacgtat ttgctgtgcg ggtaaattat ctgctttatc gggcacaaaa
    55981 agagcgcgat gaaacattct atgttggaac gcgtttcgac aaagttcgcc gtctggaaga
    56041 tgacaactgg cgcttgctgg aacgggatat cgtcctggat caagcggtaa tcacttccca
    56101 taacctgagt gtactgttct gatgaatcga atttatgcgt gtcccgttgc ggatgtgccg
    56161 gagggtgagg ctctccggat cgatacctcg cccgtcatcg ccctgttcaa cgttggcggc
    56221 gagttttatg ccattaacga tcgttgcagc catggtaatg cgtcgatgtc agaagggtat
    56281 ctggaagatg acgccacggt ggagtgcccg ctacacgccg ccagtttttg cctgaaaacg
    56341 gggaaagcgt tatgcctgcc cgccaccgat ccgctcacca cttatccagt acacgttgaa
    56401 ggtggtgaca ttttcatcga cttaccggag gcgcagccat gagcgatctg cataacgagt
    56461 ccatttttat taccggcggc ggatcgggat tagggctggc gctggtcgag cgatttatcg
    56521 aagaaggcgc gcaagttgcc acgctggaac tgtcggcggc aaaagtcgcc agtctgcgtc
    56581 agcgatttgg cgaacatatt ctggcggtgg aaggtaacgt gacctgttat gccgattatc
    56641 aacgcgcggt cgatcagatc ctgactcgtt ccggcaagct ggattgtttt atcggcaatg
    56701 caggcatctg ggatcacaat gcctcactgg ttaatactcc cgcagagacg ctcgaaaccg
    56761 gcttccacga gctgtttaac gtcaacgtac tcggttacct gctgggcgca aaagcctgcg
    56821 ctccggcgtt aatcgccagt gaaggcagca tgattttcac actgtcaaat gccgccaggt
    56881 atcctggcgg cggtggcccg ctgtacaccg ccagtaaaca tgccgcaacc ggacttattc
    56941 gccaactggc ttatgaactg gcaccgaaag tgcgggtgaa tggcgtcggc ccgtgtggta
    57001 tggccagcga cctgcgcggc ccacaggcgc tcgggcaaag tgaaacctcg ataatgcagt
    57061 ctctgacgcc ggagaaaatt gccgccattt taccgctgca atttttcccg caaccggcgg
    57121 attttacggg gccgtatgtg atgttggcat cgcggcgcaa taatcgcgca ttaagcggtg
    57181 tgatgatcaa cgctgatgcg ggtttagcga ttcgcggcat tcgccacgta gcggctgggc
    57241 tggatcttta aggaagcacg atgaaagaaa aaacgatcat tattgtcggt ggcgggcaag
    57301 cggcggcaat ggctgcggcc tcgctacgcc agcaagggtt caccggtgag ctgcatctgt
    57361 tttccgatga gcgacatctt ccttatgaac gacctccact ctcgaaatcc atgttgctgg
    57421 aagattcccc gcagttacag caggtgttac ccgctaactg gtggcaggaa aacaatgttc
    57481 atctgcattc cggtgtaacc atcaaaacgc tgggtcgcga cacacgagag ttagtgttaa
    57541 ccaacggcga aagctggcac tgggatcagc tttttatagc aatcggcgcg gcagctcgac
    57601 cgctgccgtt gcttgatgca ctgggagaac gctgctttac cctacgccat gccggtgatg
    57661 ccgccagact gcgagaagtt ctgcagcccg aacggtcagt cgtgattgtc ggtgccggaa
    57721 ctattggtct ggaactggct gccagcgcca cgcagcgcag atgtaaggtg acagtgattg
    57781 aactggcggc aaccgtcatg ggccgtaatg caccaccgcc cgtgcaacgc tatcttttac
    57841 agcgccacca gcaggctggt gtgcgcattc tgctcaataa tgccattgaa catgtggtcg
    57901 atggtgaaaa agtagaactg acgctgcaaa gtggggaaac gcttcaggct gatgtggtga
    57961 tttacggtat tggtatcagc gccaacgagc aactggctcg cgaggccaac cttgatactg
    58021 ccaatggcat tgtcattgat gaggcttgcc gcacctgcga tcccgcgatc tttgccggtg
    58081 gcgatgtggc aatcactcgt cttgataatg gtgcactaca ccgctgcgaa agctgggaaa
    58141 acgccaataa ccaggcgcaa attgccgctg ccgcaatgtt agggctaccg ctaccgctac
    58201 tgccgccgcc gtggttctgg agcgatcagt acagtgataa cttacagttt attggcgata
    58261 tgcgtggcga tgactggctt tgtcgtggca acccggaaac tcagaaggcg atttggttta
    58321 atctgcaaaa cggcgtgctt atcggtgcgg tcacgctgaa tcaggggcgt gagattcgcc
    58381 caattcgcaa atggatccag agcggcaaaa cgtttgatgc gaaactgctg atagatgaga
    58441 acatcgcgct taaatcactg taaccaggat aattagcgaa tatctcaacg cctggggcgt
    58501 ggcgaggtgc aagagtgtgt attacgttta aatcacatta tcttgcaaag ggattggtta
    58561 tgaacacact ac
//
LOCUS       DAETQN010000011        57430 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.011, whole genome shotgun sequence.
ACCESSION   DAETQN010000011 DAETQN010000000
VERSION     DAETQN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.011"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            145..1488
                     /gene="argG"
                     /locus_tag="K0W68_000719"
     CDS             145..1488
                     /gene="argG"
                     /locus_tag="K0W68_000719"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBI3072373.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1496..3103)
                     /locus_tag="K0W68_000720"
     CDS             complement(1496..3103)
                     /locus_tag="K0W68_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBI3072374.1"
                     /translation="MLEDFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFFVLTILV
                     VKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDPDEVVKM
                     LGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQFAYKDA
                     KNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3581..3667)
                     /locus_tag="K0W68_000721"
     tRNA            complement(3581..3667)
                     /locus_tag="K0W68_000721"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3631..3633),aa:Leu,seq:gag)
     gene            complement(3682..4014)
                     /gene="secG"
                     /locus_tag="K0W68_000722"
     CDS             complement(3682..4014)
                     /gene="secG"
                     /locus_tag="K0W68_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBI3072375.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4242..5579)
                     /gene="glmM"
                     /locus_tag="K0W68_000723"
     CDS             complement(4242..5579)
                     /gene="glmM"
                     /locus_tag="K0W68_000723"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBI3072376.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5572..6420)
                     /gene="folP"
                     /locus_tag="K0W68_000724"
     CDS             complement(5572..6420)
                     /gene="folP"
                     /locus_tag="K0W68_000724"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBI3072377.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6510..8444)
                     /gene="ftsH"
                     /locus_tag="K0W68_000725"
     CDS             complement(6510..8444)
                     /gene="ftsH"
                     /locus_tag="K0W68_000725"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBI3072378.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8544..9173)
                     /gene="rlmE"
                     /locus_tag="K0W68_000726"
     CDS             complement(8544..9173)
                     /gene="rlmE"
                     /locus_tag="K0W68_000726"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBI3072379.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9299..9592
                     /gene="yhbY"
                     /locus_tag="K0W68_000727"
     CDS             9299..9592
                     /gene="yhbY"
                     /locus_tag="K0W68_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBI3072380.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9748..10224)
                     /gene="greA"
                     /locus_tag="K0W68_000728"
     CDS             complement(9748..10224)
                     /gene="greA"
                     /locus_tag="K0W68_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBI3072381.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10472..11905
                     /gene="dacB"
                     /locus_tag="K0W68_000729"
     CDS             10472..11905
                     /gene="dacB"
                     /locus_tag="K0W68_000729"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBI3072382.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12091..13263)
                     /gene="cgtA"
                     /locus_tag="K0W68_000730"
     CDS             complement(12091..13263)
                     /gene="cgtA"
                     /locus_tag="K0W68_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBI3072383.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13279..14244)
                     /locus_tag="K0W68_000731"
     CDS             complement(13279..14244)
                     /locus_tag="K0W68_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI3072384.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14371..14628)
                     /gene="rpmA"
                     /locus_tag="K0W68_000732"
     CDS             complement(14371..14628)
                     /gene="rpmA"
                     /locus_tag="K0W68_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBI3072385.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14649..14960)
                     /gene="rplU"
                     /locus_tag="K0W68_000733"
     CDS             complement(14649..14960)
                     /gene="rplU"
                     /locus_tag="K0W68_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBI3072386.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15219..16190
                     /gene="ispB"
                     /locus_tag="K0W68_000734"
     CDS             15219..16190
                     /gene="ispB"
                     /locus_tag="K0W68_000734"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBI3072387.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16419..16697
                     /gene="sfsB"
                     /locus_tag="K0W68_000735"
     CDS             16419..16697
                     /gene="sfsB"
                     /locus_tag="K0W68_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBI3072388.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16745..18004)
                     /gene="murA"
                     /locus_tag="K0W68_000736"
     CDS             complement(16745..18004)
                     /gene="murA"
                     /locus_tag="K0W68_000736"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBI3072389.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="K0W68_000737"
     CDS             complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="K0W68_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBI3072390.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="K0W68_000738"
     CDS             complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="K0W68_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBI3072391.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="K0W68_000739"
     CDS             complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="K0W68_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBI3072392.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="K0W68_000740"
     CDS             complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="K0W68_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBI3072393.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="K0W68_000741"
     CDS             complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="K0W68_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBI3072394.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLANCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="K0W68_000742"
     CDS             complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="K0W68_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBI3072395.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21785..22762
                     /locus_tag="K0W68_000743"
     CDS             21785..22762
                     /locus_tag="K0W68_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBI3072396.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22776..23762
                     /gene="kdsD"
                     /locus_tag="K0W68_000744"
     CDS             22776..23762
                     /gene="kdsD"
                     /locus_tag="K0W68_000744"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBI3072397.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23783..24349
                     /gene="kdsC"
                     /locus_tag="K0W68_000745"
     CDS             23783..24349
                     /gene="kdsC"
                     /locus_tag="K0W68_000745"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBI3072398.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24346..24921
                     /gene="lptC"
                     /locus_tag="K0W68_000746"
     CDS             24346..24921
                     /gene="lptC"
                     /locus_tag="K0W68_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBI3072399.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24890..25447
                     /gene="lptA"
                     /locus_tag="K0W68_000747"
     CDS             24890..25447
                     /gene="lptA"
                     /locus_tag="K0W68_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBI3072400.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25454..26179
                     /gene="lptB"
                     /locus_tag="K0W68_000748"
     CDS             25454..26179
                     /gene="lptB"
                     /locus_tag="K0W68_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBI3072401.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26227..27660
                     /gene="rpoN"
                     /locus_tag="K0W68_000749"
     CDS             26227..27660
                     /gene="rpoN"
                     /locus_tag="K0W68_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBI3072402.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27683..27970
                     /gene="hpf"
                     /locus_tag="K0W68_000750"
     CDS             27683..27970
                     /gene="hpf"
                     /locus_tag="K0W68_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBI3072403.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28088..28579
                     /gene="ptsN"
                     /locus_tag="K0W68_000751"
     CDS             28088..28579
                     /gene="ptsN"
                     /locus_tag="K0W68_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBI3072404.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28625..29479
                     /gene="rapZ"
                     /locus_tag="K0W68_000752"
     CDS             28625..29479
                     /gene="rapZ"
                     /locus_tag="K0W68_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBI3072405.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29476..29748
                     /gene="npr"
                     /locus_tag="K0W68_000753"
     CDS             29476..29748
                     /gene="npr"
                     /locus_tag="K0W68_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBI3072406.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29962..30594
                     /gene="yrbL"
                     /locus_tag="K0W68_000754"
     CDS             29962..30594
                     /gene="yrbL"
                     /locus_tag="K0W68_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBI3072407.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="K0W68_000755"
     CDS             complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="K0W68_000755"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HBI3072408.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="K0W68_000756"
     CDS             complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="K0W68_000756"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBI3072409.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="K0W68_000757"
     CDS             complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="K0W68_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBI3072410.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34631..35560)
                     /locus_tag="K0W68_000758"
     CDS             complement(34631..35560)
                     /locus_tag="K0W68_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBI3072411.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTFTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36235..40695
                     /gene="gltB"
                     /locus_tag="K0W68_000759"
     CDS             36235..40695
                     /gene="gltB"
                     /locus_tag="K0W68_000759"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBI3072412.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40708..42126
                     /gene="gltD"
                     /locus_tag="K0W68_000760"
     CDS             40708..42126
                     /gene="gltD"
                     /locus_tag="K0W68_000760"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBI3072413.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42310..42789
                     /locus_tag="K0W68_000761"
     CDS             42310..42789
                     /locus_tag="K0W68_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBI3072414.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            42806..43111
                     /locus_tag="K0W68_000762"
     CDS             42806..43111
                     /locus_tag="K0W68_000762"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3072415.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43187..43651)
                     /locus_tag="K0W68_000763"
     CDS             complement(43187..43651)
                     /locus_tag="K0W68_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBI3072416.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="K0W68_000764"
     CDS             complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="K0W68_000764"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBI3072417.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44520..45209)
                     /locus_tag="K0W68_000765"
     CDS             complement(44520..45209)
                     /locus_tag="K0W68_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBI3072418.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="K0W68_000766"
     CDS             complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="K0W68_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBI3072419.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="K0W68_000767"
     CDS             complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="K0W68_000767"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBI3072420.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="K0W68_000768"
     CDS             complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="K0W68_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBI3072421.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49043..50365
                     /gene="dcuC"
                     /locus_tag="K0W68_000769"
     CDS             49043..50365
                     /gene="dcuC"
                     /locus_tag="K0W68_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBI3072422.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="K0W68_000770"
     CDS             complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="K0W68_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBI3072423.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="K0W68_000771"
     CDS             complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="K0W68_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBI3072424.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMCLGRS"
     gene            complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="K0W68_000772"
     CDS             complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="K0W68_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBI3072425.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="K0W68_000773"
     CDS             complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="K0W68_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBI3072426.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="K0W68_000774"
     CDS             complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="K0W68_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBI3072427.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54320..54718
                     /locus_tag="K0W68_000775"
     CDS             54320..54718
                     /locus_tag="K0W68_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBI3072428.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54872..56239
                     /gene="degQ"
                     /locus_tag="K0W68_000776"
     CDS             54872..56239
                     /gene="degQ"
                     /locus_tag="K0W68_000776"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HBI3072429.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56329..57396
                     /gene="degS"
                     /locus_tag="K0W68_000777"
     CDS             56329..57396
                     /gene="degS"
                     /locus_tag="K0W68_000777"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBI3072430.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14264 a        15302 c        14254 g        13610 t
ORIGIN      
        1 gcgcattatt ttacaaaaaa cacactaaac tcttcctgtc tccgataaaa gatgattaaa
       61 tgaaaactca tttattttgc ataaaaattc agtgagagcg gaaatccagg ctcatcatca
      121 gttaattaag cagggtgtta ttttatgacg acgattctca agcatctccc ggtaggtcaa
      181 cgtattggta tcgctttttc cggcggtctg gacaccagtg ccgcactgct gtggatgcga
      241 caaaagggag cggttcctta tgcatatact gcaaacctgg gccagccaga cgaagaggat
      301 tatgatgcga tccctcgtcg tgccatggaa tacggcgcgg agaacgcacg tctgatcgac
      361 tgccgcaaac aactggtggc cgaaggtatt gccgctattc agtgtggcgc atttcataac
      421 accaccggcg gcctgaccta tttcaacacg acgccgctgg gccgcgccgt gactggtacc
      481 atgctggttg ctgcgatgaa agaagatggc gtgaatatct ggggtgacgg tagcacctat
      541 aaaggaaacg atatcgaacg tttctatcgt tatggtctgc tgaccaatgc tgaactgcag
      601 atttacaaac cgtggcttga tactgacttt attgatgaac tgggcggccg tcatgagatg
      661 tctgaattta tgattgcctg cggtttcgac tacaaaatgt ctgtcgaaaa agcctactcc
      721 acagactcca acatgcttgg tgcaacgcac gaagcgaagg atctggaata cctcaactcc
      781 agcgtcaaaa tcgtcaaccc aattatgggc gtgaaattct gggatgagag cgtgaagatc
      841 ccggcagaag aagtcacagt acgctttgag caaggtcatc cggtagcgct gaacggtaaa
      901 acctttagcg acgacgtaga aatgatgctg gaagctaacc gcatcggcgg tcgtcacggc
      961 ctgggcatga gcgaccagat tgaaaaccgt atcatcgaag cgaaaagccg tggtatttac
     1021 gaagctccgg ggatggcact gctacatatc gcttatgaac gtctgttgac cggtattcac
     1081 aacgaagaca ccattgagca gtatcatgca catggtcgtc agttgggccg tctgctgtac
     1141 caggggcgtt ggtttgattc ccaggcgctg atgctgcgtg actctctgca acgctgggtt
     1201 gccagccaga tcactggtga agttaccctg gagctgcgcc gtgggaacga ttattcaatc
     1261 ctgaataccg tctcagagaa cctgacctac aagccagagc gtctgacgat ggaaaaaggc
     1321 gactcggtgt tctcgccaga tgatcgtatt ggtcaattga ccatgcgtaa cctggatatc
     1381 actgataccc gagagaaact ttttggttat gccaaaactg gcctgctctc ctcctctgcc
     1441 acttcaggcg tgccgcaggt ggagaatctg gaaaacaaag gccagtaatt cgacatcaac
     1501 cctgcccttc aggggtgggg ttaccttgct tatcctctgt aaactccttt ctgagcatca
     1561 cataatcgaa aacatcatgg tttgcatcca caacccgtga gtctccttta tagtgtgcaa
     1621 tcacttcctc cagcgttttc ggttgttccg gctttgttac ccccatccac gcattaataa
     1681 ggtaattatt gtaagctgtc gaaaagatgt tgttttccgt tgtacgatcc acgccatccc
     1741 caagaacggg gctataccag ataaacatcg ggacatgata tgcctgctgg ctagcctccc
     1801 tgcctccatg aaaatagacg ttcttcttcg tagggtcacg ttccaggccg tggtcggcaa
     1861 aatacatgac cgaggcgcgg cgatctttta ataattcaaa aacctgacct agcaaactat
     1921 ctgtgtaatg aatggagttg tcatagcagg catcctgatc gtcctgcggt tgaaacacgg
     1981 cgctggattg cggataggcg ctacaagccg gttcatggct tccatttaaa tgaagaacaa
     2041 tcagtttctt ttgctgcgta ttttgctgta atgcttgcga taaatgcggc aacaacaatt
     2101 catcaaatcc tctgacataa actgtttcca tggcgcgcat ggcgatactg gtaactgctg
     2161 taccattctg ccgaaaagcg gattgcgagc ttagccagaa agtctgaaat cctgcttgat
     2221 tagccatatt aataatgttg tcggggtaat tatgaatgtc atgactcaaa acagaatcag
     2281 cagttaaaga aaggggaacc gacagcgcag tgtaaggtgc gccgcttatt gattgattaa
     2341 acagtttgat ctgttttctt tgtgcttcaa cttgcggtgt cgtagagcgt gtatatccat
     2401 acaaagacat attgtcgaca cgtacagact cccccacaat caacacgtag gtatcaatac
     2461 ctgtatccct gactgataat tgaaaatacg gcaccgtgtt tgcaattgag agtaatcttt
     2521 gatgctcttt cgctgctaaa gcaaaataat tgagattgaa aaacggcgta taggtagcaa
     2581 atcgcgacgc tagtatatat ggactgaatg cttttttatt ttttgcatct ttataagcaa
     2641 attgacaagc agaaaataaa ctgcccgaaa tgacaatcag caataatatt cctgtcactt
     2701 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
     2761 aagcaaaagc acatagataa gggatataca tccccagcat tttgacaact tcatccggat
     2821 cactctgcag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
     2881 aatagaggct aatactcatg tctgccgcag tcagtaacac aaatggtgcg gcaacaagca
     2941 ggcgaagcgg aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca
     3001 atcgctgaac catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa
     3061 cacaaagata caacaaccaa tgagatttaa aatcttctag caaatttgtt gaatactgtc
     3121 atccagaaat ccaagttcac aaccaatata aacaattatc aacaatgttc aattttatat
     3181 cagacatgtt tttcaactct gatccatatc agaccgaaac actgttagtt ttttcttttt
     3241 aaatgatgcg gccattgcgt tctcagtaat taaataacat tatcaatgcg tctctaaaca
     3301 ttacgcagaa cgtagccatc agtagttata ctgacaggag caggatggtt tatcgagaag
     3361 taaacatatc cgtattcgta ccggaactgg agttgacaca ataaagtgcc aattatgtca
     3421 gcagaaggga aaagtttata actaaggcgt accggcacca tcgtttcaag gtaccagcta
     3481 cgagtaaagc aactggacga gatacagata cctgacaacc attcctcaga ccaggaccaa
     3541 aacgaaaaaa gacgcttttc agcgtctctt ttctggaatt tggtaccgag gacgggactt
     3601 gaacccgtaa gccctattgg gcactaccac ctcaaggtag cgtgtctacc aattccacca
     3661 cctcggcacg gatactactt tttagttcgg gatatcgctg gtcggcttag ccggagcagc
     3721 tggctgagtt tgttcggttt tcgccggtgc actcagattt tcccattcgc tacctttatt
     3781 ggttttgttg ctattgatgt tacccagcac cagactgatg ataaagaata acgttgccag
     3841 cagcgccgtc atgcgggtca tgaagttacc agaaccactt gaaccaaaca gcgtagcgga
     3901 agcgcctgct ccgaaggagg ctcccatatc agcgccttta ccttgctgca gcatgatcag
     3961 accaacaagg ccaattgcca caataaggaa aactaccaaa agagcttcgt acataatcaa
     4021 cctgttcctt gcggagttgc cgcgtaccaa tgcttcaacc aataaagcgg gagtttttaa
     4081 tctttcccac tgaagtgggt gtgaatacta accaaagcga atgaccttcg caagggcaat
     4141 tttatcgcat tgtatcaact gcggaaaaaa acagcaaaac cgatgtgttc gctgagaaaa
     4201 aaggcgacaa tcgccgcctt tttagccagt tatttaccgt tttaaacggc ttttactgca
     4261 tcggcgatac ggtgtgcaaa ttcagttacc tgcgcttcgt cttcaccttc taccatcacg
     4321 cgaattaacg gttcggtgcc ggatttacgc aacagcacac gtccacggct acccagcgcc
     4381 gcttcaactt cggcggtcac agctttaact gactcatgct caagcggatc gccgctacct
     4441 gcggtgtaac gcacgttaac cagaatctgc gggaacattt tcatgccgct gcaaaggtcg
     4501 tgcaggctca tatggttacg tgccatcgcc gccagcacct gcaagccagc aacgatgccg
     4561 tcaccggtag tggttttatc cagcaggatc acatgaccgg aattctctgc accgatacgc
     4621 cagcctttct cctgcatttt ttccagtacg tagcggtcac ccactttcgc gcgcgcaaat
     4681 ggaattccta actgtttcaa cgccagctca agccccatgt tactcatcaa tgtacccaca
     4741 gcaccaccac gcagctggcc ctgacgaaga ccttcacgcg cgatgatata catgatctga
     4801 tcaccatcga ctttattgcc ttcatggtca accataatca cgcgatcgcc gtcgccgtcg
     4861 aaggcaatac cgagatccgc tttttcagcc agcacacgag cctggagcgc gcgaacgtcg
     4921 gtagccccca cttcggcatt gatgtttaca ccgttcggct cacaaccgat agcgataacg
     4981 ttcgccccca gttcacgcag cacgttcggc gcgatgtgat aagtcgcacc gtttgcgcaa
     5041 tccaccacaa tcttcagttc gctgaggcta agttcgttcg ggaacgtggc tttgcaaaac
     5101 tcgatatagc gacccgcagc atcaacgata cggctggctt tacccagttc tgccgaatca
     5161 acacagctga tctctttttc catttccgct tcgatggcct cttctaccgc atccggtagt
     5221 ttggtgccgt cgatagagaa aaatttaatg ccattatcgt agaacgggtt atgcgatgca
     5281 gatatcacaa ttccggcctc tgcgcggaag gtacgcgtca gataagccac ggccggtgtt
     5341 ggcatcgggc cagtgaagag tgcggaaagg cccgctgccg ccagacccgc ttccagtgct
     5401 gactccagca tatagccaga aatacgcgtg tctttaccaa taatgatctt acgggagccg
     5461 tggcgcgcca gcactttacc cgcggcccaa cccagcttaa gcacaaaatc aggtgtgatc
     5521 ggcgcatccc ctacacgacc acgaatccca tcggtaccga aatatttacg attactcata
     5581 gcgtttgttt tcctttgcag acagagtggc ttccaccacc cgcatcgctt ctacggtttc
     5641 tttgacgtca tgaacacgaa taatgtgcgc gccttgcatt gcggcaatga ccgcacaggc
     5701 cagactaccg ctcaggcgct cggacggccc cacgttcagc agctgcccaa tcatcgattt
     5761 tcgtgacata cctaccaaca gcggcaggtt gaaatggtga aattcagcca ggcgcgccag
     5821 taatgaatag ttatgggaga gatttttacc gaaaccgaat ccggggtcga gcaacaattt
     5881 ctcttttgcg atacccgcct gctcgcaacg tgctatttgc tcaataaagt agcgattcac
     5941 ttctgcaaag acatcgtcat acttcggagc ttcctgcatg gtttttggat ttccctgcat
     6001 atgcatcaga caaaccggta aaccggtttc tgcagccgcc tccagagcgc caggttcgga
     6061 aagggagcgg atatcgttaa taatgtgagc gccaactttc gctgactcac ggatgacttc
     6121 tggtttggat gtatctaccg agatccagac ttcgaagcgt tgagcaattg cctcaaccac
     6181 aggaataaca cgttgcaact cttcttcaac gctaacttcc gccgcccctg ggcgcgtgga
     6241 ctcgccacca acgtcaatga tcgtcgcgcc agcgttgatc atcagattcg catgtttcac
     6301 cgcatctatc agcgagttat gcgtgccacc atccgaaaag gaatcaggcg tgacgttgag
     6361 gatccccatt acgtgaggat ggctaaggtc cagtgaagtg ccctgggcaa agagtttcat
     6421 ggtgttatcc ctggtatgaa ttgataagaa aaaaccccgg agcacgcccc ggggttttcg
     6481 gtacaaatac agtcatctga tgcgggaact tacttgtcgc ctaactgctc tgacatggtg
     6541 ttacccgggt tcggcgtacg cggttcatca accggacgag gagcctttgg actaccattg
     6601 tcgccagaat tgttagaagc gcctggttct tcccagcccg ctggcggacg tacatcgcga
     6661 cgtgccatca ggtcatcaat ctgcggtgcg tcgatagtct catatttcat gagagcatct
     6721 ttcatcgcat gcagaatatc catattgtcg gtcagaagct gacgcgcacg attatagtta
     6781 cgctcaatca gtgctttcac ttcctggtcg atgatacgtg cagtttcatc ggacatatgt
     6841 ttcgctttcg ctacgctacg gccgaggaac acttcacctt cttcttccgc gtacagcagt
     6901 ggacccaatt tctcagagaa gccccactga gtcaccatgt tacgcgccag gttggtcgca
     6961 actttaatat cgttggacgc accggtagaa acatgttccg gcccgtagat gatctcttct
     7021 gccagacgac caccgtacag cgtagaaatc tggctttcca gtttctgacg gctggcgctg
     7081 attgcgtcgc cctcaggcaa gaagaaagtc acacccagcg cacgaccgcg tgggataatc
     7141 gtcactttgt gcaccggatc gtgttccggc accaggcgac cgataatcgc atgacccgct
     7201 tcgtggtaag ccgtcgattc tttctgcgct tccgtcatca ccatggagcg acgttccgca
     7261 cccatcatga ttttatcttt cgctttctcg aactcaacca tcgacacaac gcgtttgttg
     7321 ccacgagcag cgaacagtgc cgcttcgttt accaggttcg ccaggtcagc accggagaaa
     7381 ccaggagtac cacgggcaat gattgccgcg tcgatatcgg gtgccaatgg tacgcgacgc
     7441 atgtgaactt tcaggatctg ctcacgaccg cgaacatctg gcaagccaac cacaacctga
     7501 cggtcgaaac ggccaggacg cagcagggcc gggtcaagaa cgtccggacg gttagtcgcg
     7561 gcgataacga tgataccttc gttaccttcg aagccatcca tctcaaccag catctggttc
     7621 agagtctgtt cacgttcatc gtgaccaccg cccagaccag cgccacgctg gcggcctacg
     7681 gcgtcgattt catcgataaa gatgatgcac ggtgccgctt tcttcgcctg ttcgaacatg
     7741 tcacgaacac gggatgcacc cacaccgacg aacatttcta cgaagtcaga accggagata
     7801 gtaaagaacg gaactttcgc ttcgcctgca atcgctttcg ccagcagcgt tttaccggta
     7861 cccggaggac cgaccatcag gacgcctttc gggatcttac cgccgagttt ctggaagcgg
     7921 ctcggctcgc gcagatattc aaccagttca gcaacttctt cttttgcttc gtcgcagccc
     7981 gcaacgtcag caaaggtcgt tttgatctga tcttccgtca gcatgcgcgc tttgctctta
     8041 ccaaacgaca tggcaccttt gccaccgccg ccctgcattt gacgcatgaa gaagatccac
     8101 acaccaatca gcaacagcat cgggaaccag gagatgaaga tagaagccag caggcttggt
     8161 tcttcaggcg gttcaccgac aaccttgacg ttcttggtca acaggttatc cagtaatttc
     8221 ggatcctgaa ccggaatgta agtggtataa cggttactat ctttcttggt aacgttgatt
     8281 tcacgtccgt tgatacgcgc ttcacgaacc tggtcgttat tgacctcttg taggaaggta
     8341 gagtaatcca ccttacggcc attagactcg ctgggcccaa agctctggaa tactgacatc
     8401 agcacaacgg caatgaccag ccagagtatt aggtttttcg ccatgtcact caagggatta
     8461 acctcttatt acaactgtgt taaaaacagc gtcaggatac tctatatcca gcatctttca
     8521 aactttcgtc tgaaatctcc cggttagggt ttacgcccgg tcgctacaat atacacttcc
     8581 cgcgaacgtg cacgagaaga gtccggctta cgaactttga ccttcgtaaa cagggagcga
     8641 atttccctta gatactcatc gaaaccttcg ccctggaaca ccttcactac aaaactgcca
     8701 cctggcgcta atacatcacg acacatttct agcgccagtt ccaccagata catggcacgg
     8761 gggatatcca ccgccggtgt tccgctcatg tttggtgcca tatcggacat gacaacctgg
     8821 actttgctgt cgccaacgcg ctccagcagt gctttcatca ccagttcatc acgaaaatcg
     8881 ccctgaagaa agtctacacc aacgatagga tccataggta aaagatcgca agcgatgatg
     8941 cggcctttgc cgccaatttg ggtgaccaca tattgtgacc aaccacccgg agcagcacca
     9001 aggtcgacaa ccgtcattcc cggtttaaag agtttgtcac tttgctgtat ttcatcaagt
     9061 ttaaaccagg cacgggaacg taaccccttt ttctgtgcct gttgaacata tttatcgcta
     9121 aagtgttcct gaagccagcg gctggagctg gcagaacgct tcttacctgt catttaactt
     9181 tcccatgggg ataactcatc gtaaccaatt gcgtaaattt ttacgcgcct atttggtgat
     9241 atatgggaga tggcggtaga atgacccatt ttcaatccca acgtaagcaa aaaatacgat
     9301 gaatctgagt actaaacaaa aacagcacct gaaaggtctg gcacatccgc tcaagccagt
     9361 tgttctgctt ggcagtaatg gtttgaccga aggggtgctg gccgagattg aacaagcgtt
     9421 agagcaccat gaactcatca aggtgaaaat cgccaccgaa gatcgcgaaa caaaaacctt
     9481 gatcgtggaa gctatcgtgc gcgaaaccgg cgcctgtaat gtacaggtca tcggtaaaac
     9541 gctggtgctt tatcgcccaa ctaaagaacg taaaatctcg ctgccacgct aagattatcc
     9601 taaagttaca cacatttgct gtgtaaaacg aggggttttc cgcaggcagg agagcaaaat
     9661 gccacgctct gttcgttgat aaaaggccgc atagcggcct ttttcctttc tttacaatac
     9721 atcaacatct tgagtattgg gtaattctta caggtattcc accttaatta cttcaaattc
     9781 tacttcgccg cccggcgttt tgatgaccac aacatcatct tcttctttgc cgatcaggcc
     9841 acgagcaata ggcgagttta cagaaatcag gttttgttta aagtcagctt cgtcatcgcc
     9901 aacgatgcga taagtctgtt cttcgtcaga atccagattc agcaccgtta cggtagcacc
     9961 aaaaataacg cgcccattgt taggcatttt ggtgacatca atcacctgcg cgtttgacag
    10021 cttggcttcg atgtctttga tacggccttc gcagaagccc tgctgttcac gagctgcgtg
    10081 gtattcggcg ttttctttca ggtcgccatg ctcacgcgct tccgcgatag cagcaatgat
    10141 ttcaggacgg cgcacagatt tcagaaaatc cagctcttcg cgtaattttt cagcgccgcg
    10201 taaggtcatc ggaatagctt gcatttgtta tacctcttga atattcctga tggggcaagt
    10261 cttcacccac cccggctgtt cggcctgccc ggcataactc ctcaccggga ccagaagcaa
    10321 aaaaataccg acccaggtac aagtcccagg tcagctacaa ttcacatttt gatagtcatt
    10381 ttaccctgaa gttcccgaag ggtcatcgtt tactttatag ggcgttgcgc cgtagtatga
    10441 cggctcgatt ccaggttgtt agcgcgagat tatgcgattt tccagattta tcatcggatt
    10501 gaccagctgt atagcgttca gtgttcaggc cgcaaatgtt gatgagtaca ttactcaact
    10561 ccccgctggt gccaaccttg ccctgatggt gcaaaaagtc ggcgcgtcgg cccccgctat
    10621 tgattaccac agtcagcaga tggcgctgcc tgccagtacc cagaaagtga ttactgcgct
    10681 ggcggcgttg attcaactcg gccccgattt tcgttttacc acgacgcttg aaaccaaagg
    10741 caatgtggaa aacggcgtac ttaagggtga cttagtggcg cgatttggtg ccgatccgac
    10801 gttaaaacgt caggatattc gcaatatggt cgcgactttg aaaaaatctg gcgtcaacca
    10861 aatcgatggc aatgtgttga tagatacctc cattttcgcc agccacgata aagcccccgg
    10921 ctggccatgg aatgacatga cacaatgctt tagcgctccg cctgccgccg ccatagttga
    10981 ccgcaactgt ttctccgtct cgctctacag tgcccaaaaa cccggtgata tggcctttat
    11041 acgcgtggca tcttattacc ccgttacgat gttcagccag gtacgcaccc tcccccgtgg
    11101 ttctgccgaa gcgcaatact gcgaactgga tgtcgtacca ggcgacctga accgctttac
    11161 gctgacggga tgcctgccac aacgttctga gccgctcccg ttggcttttg ccgtgcagga
    11221 tggagccagt tatgccggtg caattctgaa agatgagtta aaacaggcgg gtatcacctg
    11281 gagcggaaca ctgctgcgcc agactcaggt taacgaacct ggaacggtag ttgccagtaa
    11341 acagtcggcc ccgctgcacg atctgcttaa gattatgctg aaaaagtcgg ataacatgat
    11401 cgccgatacg gttttccgca tgataggcca tgcgcgcttc aatgtacctg gtacatggcg
    11461 ggccgggtcg gacgctgtgc gtcagatcct gcgccagcaa gccggtgtcg atattggaaa
    11521 caccattatt gccgatggtt cagggctttc gcggcataac ctgattgccc ccgccaccat
    11581 gatgcaggtg ctgcaataca ttgcccaaca cgacaatgaa cttaacttta tctccatgct
    11641 gccgctggcg ggctatgacg gctctttgca gtaccgtgca ggtctgcatc aggcgggcgt
    11701 ggatggaaaa gtctcagcga aaaccggttc gttgcagggg gtatataacc tggcgggatt
    11761 cattaccaca gcgagcgggc aacgaatggc gtttgtgcaa tatctttctg gctatgcagt
    11821 agaacctgcg gatcagcgta atcgccgtat tccgttagtg cgttttgaaa gccgtttgta
    11881 taaagatatt tatcagaaca attagtcaaa aagaaacccc ggcacatggc cggggcttca
    11941 gattattgac aaagtgcgct ttgtttatgc cggatgcggc gtaaacgcct tatccggcct
    12001 acaaaatcgt gcaaattcaa catattgcaa ttctcttgta ggcctgataa gcgtagcgca
    12061 tcaggctgat ttggcgttta tcatcagtga ttaacgcttg taaatgaact caacgccttc
    12121 ttcgtcgtct tcgtcccagt cgtcatccca gtcttcatca tcctcttcag caatctcttc
    12181 aagctgctgg cgatgataat cgtcccacat gaattcgact ttctctggct gtttcgcttc
    12241 ttcagcctgc acgaccgggt tttcaaggat aaaggtcatc acatcccagc agagatcttt
    12301 cacgcccagt ccgctcgccg cagagatcag ataatattta tcttcccagc ccagcgcttc
    12361 agcaatcgct ttcgctttct cttcagcttc tgccttatcc agcagatcga tcttattgaa
    12421 cactaaccaa cgcggtttcg ccgccagatc ctggctgtat ttttccagct cgctgataat
    12481 aatacgcgcg ttttcaaccg gatcggtgcc gtcaatcgga tcgatatcga tgaggtgcaa
    12541 caggacgcgg caacgttcca ggtgcttcag gaagcggata cccagacctg cgccttccgc
    12601 agcgccttca atcagtcctg gaatatcggc aacaacgaag ctcttttcgt tgtccattcg
    12661 taccacaccc agacttggca ccagagtggt aaacggataa tccgccactt tcggtttagc
    12721 cgccgatacc gcgcgaataa aggtcgattt acccgcgttt ggcatcccca acataccgac
    12781 gtcagccagc agcatcagct ccagcagcag ctcacgctta tcacccggcg taccgttggt
    12841 tttctgccgt ggtgtacggt taacggacga tttgaaacgg gtattgccca gaccgtgcca
    12901 gccgccctta gcaaccagca gacgctgacc gtgtttagtc atatcgccca tggtttcacc
    12961 agtaccctgg tcgattacac gcgtacctac cggcacttta atcgtcacgt ctttaccgcg
    13021 cttaccggta caatcgcggc ttgcgccgtt ctgaccgcgc tctgcacgga aagatttttc
    13081 aaaacgataa tcgataagcg tgttcaggtt ttcgtcggct tccatccata cgtcaccacc
    13141 atcaccaccg tcgccgccat ccgggccgcc tttcggaata tacttttcgc ggcggaagct
    13201 cacgcaacca ttaccgccat cacctgcaac gaccagaatc gatgcttcat caacaaactt
    13261 cattttattc tccataaatc attcgcctgc gcggggttgc gataccaccg ttgtatgctt
    13321 acgtaatccg ccccaaatac gatgaccaat ggcggaatac atcgcgcccg caaccacgac
    13381 aaacgcaccg agataaccta aaaggtttaa catcggtctg gcgaagaaat cgggccaggc
    13441 cagtgataaa agatctgaaa aaaacagcgt aaacagtggg gtgagcgtga tgatcgcgct
    13501 cacctgcgct gcctgccagc gagccatcgc ttccgccagg gcgccatatc ctaccaatgt
    13561 attcagtccg caaaaaatta aacatgcgag ctgccagtgg ctaagctgcg ctatcactcc
    13621 aggctttgcc agagggaaga gcgcaattgt acataaagtg tacagtaaaa acaggatctg
    13681 cggtgaggcc agccgacgca ataaaacctt ttgcgccacg ccataactca cccaaaccgt
    13741 cgccgcaccg accccaaaga taactcccca ggtgtaatcg gtgagctttg taaatatctc
    13801 gaccagactg gtgttaaaaa acatcaccag gccgctcagg agcatcaatg cccctacaac
    13861 ctgagtgctg cgcattttct ctttcaggat aaatacgctg gcaaccatca tgccaactgg
    13921 cgagagttgc ccaatcacct gcgaagcggt cggactcagg tattgcaagg atgagctgaa
    13981 caggatgaag ttcccaaaca gcccggcggt cgccactgcc aacaaaatca accagcgtgg
    14041 cttacgaaac acgcgtaatg gcggcaacct cttcttcacc gcaagaatgg caccaaggcc
    14101 aatactcgcc atcaagaaac ggtaaaacac gattgtcgga ggttccatca cctccagcac
    14161 ctgcttcatt gcgattggca acgcccccca gcaaattgct gtggtgagcg ccaaaagaat
    14221 gccaatgcct gcctgctgct tcataccgta ttccctgcaa agcgcatttg cggattaacg
    14281 tcgcaccatg cgcattttta ccggttatcg aatgtaaaaa gccccgcaac gtgttgcggg
    14341 gctttcatcc gttaccggga cgcgaaaaac ttattcagct tcgatgctga taaatttacg
    14401 gtttttcggg cctttaactt cgaatttcac tttaccgtct gctttagcaa acagagtgtg
    14461 gtcacgaccg caacctacgt tagcgccagc gtggaatttg gtaccacgtt gacgaacgat
    14521 gatgctaccc gccagaacgg attcgccacc gaaacgctta acgcccaggc gtttagcttc
    14581 tgaatcgcga ccgttacgtg tggagccgcc agccttttta tgtgccattt gaaatctctc
    14641 ctcaggtctt aggcgctgat gccagtaatt ttcacatcag tgaaccactg acgatgaccc
    14701 tgctgcttac gatagtgttt acgacgacga aacttaacga ttttaacttt ctcgccacga
    14761 ccgtgagcaa caacttcagc tttgattacg ccgccatcaa cgaaaggaac gccgattttg
    14821 acttcttcac cgtttgcgat catcagcact tcagcgaact caacagtttc gccagttgcg
    14881 atgtccagct tttccaggcg aacggtctga ccttcgctta ctcggtgttg tttaccacca
    14941 ctttggaaaa ccgcgtacat ataaaactcc gcttccgcgc acaccttttc aatgattcag
    15001 agtgcgctat aaatattcac aatagggcgc gaatattacg caaaacgcac gcctttgaca
    15061 agtgctacag tcaatacacg aagaaaaaaa acacaacttg tacggtaacg tttatctgtg
    15121 ccattttttc agtacaatca ccctatattc ctaaccataa accctaagtt gcctttgttc
    15181 acagtaaggt aatcggggcg aaaagcccgg cttttgcgat gaatttagaa aaaatcaatg
    15241 agttaaccgc gcaagatatg gcgggtgtta atgcggcaat ccttgagcag cttaattccg
    15301 acgtccaact gatcaatcag ttaggctatt acatcgtcag cggcggcggt aaacgtattc
    15361 gtccgatgat tgctgtactg gctgcacgag ctgttggcta tgagggaaat gcgcatgtca
    15421 ccattgctgc cctgatcgag tttatccaca cggcgactct gctacacgac gacgttgtgg
    15481 atgaatcaga tatgcgcagg ggtaaagcta ccgccaacgc cgcatttggc aatgccgcca
    15541 gcgtgctggt aggcgatttt atttataccc gcgcgttcca gatgatgacc agcctcggtt
    15601 cactcaaagt gctggaagtc atgtcagaag ctgtaaacgt catcgcagaa ggtgaagttc
    15661 tgcaactgat gaacgttaac gatccggaca tcactgaaga aaactacatg cgcgttatct
    15721 atagcaaaac cgcgcgtctg tttgaggctg ccgcgcagtg ttccgggatt ctggctggct
    15781 gtacgccgga ggaggagaaa ggcctgcagg attatgggcg ctatctcggc actgctttcc
    15841 agttgatcga cgatttactc gattacaatg ccgatggcga acagttaggt aaaaatgtcg
    15901 gcgacgatct gaacgaaggt aaaccgacgc tgccgctgct gcatgcgatg catcatggca
    15961 caccagaaca ggcacagatg atccgtaccg ccatcgaaca gggtaacggt cgccatcttc
    16021 tggaaccggt tctggaagca atgaacgctt gtggatctct tgaatggacg cgtcagcgtg
    16081 ccgaggaaga agcagacaaa gccatcgcag cattacaggt gctcccggac accccttggc
    16141 gagaagcact catcggcctc gcgcacatcg ctgttcaacg cgatcgttaa tcccctccct
    16201 tcatcccgcg cagtgcgcgg gatgattcca gtaaattcca taaaaacact aattcagcgc
    16261 tataacacct gaaaaaaaag ggcgatgaat ctttatatat tctgaatatt cacactcttt
    16321 acaggaactt tttagagcaa taggccatca ggagtatagt gatgcccgac agaagaagtg
    16381 ttctgaatga aagcgactaa cttaaggagt gaggaaagat ggaaagtaat ttcattgact
    16441 ggcatcccgc tgacatcatt gcgggtttgc gcaagaaggg aacgtcaatg gcggcggagt
    16501 ctcgcagaaa tggtttgagt tcctcaacgc tggcaaacgc attatcgcgc ccatggccga
    16561 aaggagagat gattattgcg aaagccctgg gaactgaccc ctgggttatc tggccatcac
    16621 gctaccatga tccgcagacc catgagttta tcgacagaac gcagttgatg cgtagctaca
    16681 ctaaaccgaa aaaatgagtg gtctggcggt agccccgcga acggggctgc cagctctcag
    16741 acgattactc gcctttcaca cgctcaatat ttgcacctaa agcgcgcagt ttgtcttcaa
    16801 tgcgttcgta gccacgatcg atgtgataaa tacgatcaac caccgtcgtc ccttccgcaa
    16861 tacagccagc cagcaccagg cttgctgatg cacgcagatc ggttgccata acctgtgcgc
    16921 cagaaagttt ttcaacaccg tgacaaataa cggtattgct ttcgatttcg gcatgcgcgc
    16981 ccatacggct aagctccggc acatgcataa agcggttttc aaagaccgtt tcggtgatga
    17041 atccggtccc ttctgccacc aggttcaaca gcgtgaactg ggcctgcata tcggtcggga
    17101 atgccggatg cggcgcggta cgtacgttaa cagccttcgg acgtttgcca tgcatatcca
    17161 ggctaatcca gtcttcgccg acttcgatgt ccgctccagc gtcacgcagt ttcgccagca
    17221 cggcgtcgag agtatctggc tgcgcgttac ggcagataat tttgccgcga gaaatcgccg
    17281 ccgccaccag gaaagtaccg gtttcgatac gatccggtag cacgcgatag acaccgccgc
    17341 ctaaacgttc cacaccttcg atgacgatac gatcggtgcc ctgaccgcta attttcgcac
    17401 ccagcgtaat caggaagttc gcggtatcga cgatttccgg ttcacgcgct gcattttcaa
    17461 taatcgtggt accttccgcc agggttgcag cacacatgat ggtcaccgtt gcgccaacgc
    17521 tgactttatc catcacgata tgtgcgcctt tcagacgacc atcgacggaa gctttaacgt
    17581 aaccttcttc cagtttgatg gtcgcgccta attgttcgag gccagaaatg tgtagatcaa
    17641 ccggacgcgc accgatcgta caaccgccag gcagtgaaac ttgcccctga ccaaagcgcg
    17701 ctaccagcgg ccccagtgcc cagatagaag cacgcatggt tttaaccaga tcgtaaggtg
    17761 cgcagaatac attaacgtcg cgggcatcaa tatgcacaga accattacgt tctactttcg
    17821 cacccagctg gcttagcagc ttcattgatg tatcgacgtc tttcagtttt gggacgttct
    17881 ggatctctac cggttcttcc gccagtagag cggcaaaaag gataggcaga gcagcatttt
    17941 tagcgccgga aattgtgact tcgccctgga gcttcgttgg cccctgaaca cgaaatttat
    18001 ccatttagtt tgttctcagt taacaattca tatccgctac cggcgaatcg cccatagctc
    18061 aaaagccgtt cagtttgcga tcgcgcgccc actccgcagg ggtatacgct ttgatcgaca
    18121 cagcatgaat gcggttatcc gcaatatatt ccatcagcgg gccatagacc gtctgctgtt
    18181 ttttaacccg actcatgccg tcaaacaact cacccacggc aataacctga aagtggctgc
    18241 catcgccgga aacgtggact tcctggaggg agagagcgtt catcaacacg ctctgaattt
    18301 cattattttc catgggatct tcaatcatca gttaataaac cagcgaaaca tcttagagca
    18361 aagttgcgct ggcataaata agcaaaaagc ctcgctgata aatcagacaa ggctcgactt
    18421 gcaggcaggt ttgccggaca ggcggttaac gccatatccg gcctgaaaaa atttaacgag
    18481 gcagaacatc agcaggcaaa ttatacaatt tcgcgagggt atacactttg tcgtttaccc
    18541 cctgaagcgt cacgttgttg ccctgctttt tcgccagatc gataagatgg agcagcagtg
    18601 ccagtccacc cgtatccacg cgggacacac ggctaagatc gatgcaggta atccccttca
    18661 ccgcttcctc acgcatttcc caaagcggta gcagaacgtc ctgatccagc tctccggata
    18721 acgccagcgt gtcacccgtc tgcatccagc tcagtgactc gctcattatt ttttctcttc
    18781 cagagtgatt ttctgttgag aaatcgattt cagttgcgca gtcaggccgt cgataccttt
    18841 ggtacgcagc agcgttcccc actcgttttg tttggtggtg atcatactga cgccttcagc
    18901 aatcatgtcg taagcctgcc agtttcccgt ctgggagttt ttacgccact ggaagtccag
    18961 acgcaccggc ggacggccat tcgggtcaat aatggtaacg cgaataggca caatggtttt
    19021 atcgcccagc ggctgttctg gcgcaatctg ataggtttga ccgtgataca tcgccagcgc
    19081 ctgaccgtaa gcctgcttca ggtactcacg gaaagcggca aagtaggctt cacgttgagc
    19141 aggggtcgca ctcttgtaat actggcccag caccagcgca ccggcgtatt tcacctgtac
    19201 gtatggcagc agttcctgat caacaatggt acgcagataa tccgggttgg cccgaatttg
    19261 cggttgctca ttcttcaggc gatcgaacgt tttctgcgcc gcctcgtcca tcagcttata
    19321 aggattggtc tggtctgccg cggttgccgc actcagaggt gcaatcacca gcaaagcgac
    19381 catcattaaa cgtttaaaca tgcgtcggtt ctcctgaaat tatttcgttg tacccacagg
    19441 ttcagtggtt tcattattac ctggcgcagc agctggcgca tcgccactat tcttattgtc
    19501 atcgccttta ctaccgtaaa ggaactgacc aatgagatct tccagcacca tcgcagactt
    19561 agtgtcctga attgtatcgc catccttcag gatagcagtc cccagttccg ggtcttcaaa
    19621 accgacgttt aatgccagat attgttcccc cagcaggccg gaagtacgaa tgctcagcga
    19681 actggtatct ggaatgtggt tataacgttg ttcaatttcc agcgttacgc gcggcagata
    19741 ggttttcggg tccagcgtaa tatccgccac ccgacccaca acaacgccac caatactgac
    19801 cggagagcgc gctttcaggc cgccaatgtt atcgaacgtc gcataaagcg tgtaggtcgg
    19861 ttcagtacgt atggacgtca cgttcgccgc cttcaggcaa acaaacagcg ccgccagcag
    19921 tgctgctaat aaaaagatac ccacccaaat ttcatttttt ttcgtttgca tgaactcaat
    19981 tcccaaacat caatgcggtc agcacaaaat ccagccccag aacagccaga gacgagtgga
    20041 caacggtgcg agtggttgcc cggctaatcc cggcagacgt cgggatggtg tcgtagccgt
    20101 taaacaacga aatccacgtc accgtgatgg cgaacaccac gctcttaatc agacagttgg
    20161 ccagatccat acgccagtcg acggcatttt gcattgccga ccagaagaac ccgctatcaa
    20221 tgcctttcca actgacgccg accagagatc cgccccagat ccccacggcg acgaaaataa
    20281 ccgtcaacag tggtaatgaa ataaccccag cccagaaacg gggagaaata acccgacgca
    20341 gcggatccac cgccatcatc tccatactgg agagttgctc tgtagcgcgc atcaggccga
    20401 tttctgcggt tagcgccgaa ccagcacgcc cggcaaacaa caacgcggca accaccggcc
    20461 ccagttcacg cagtagcgat aacgccacca gcatacccag actggtttcc gcactataag
    20521 tggtcagaac cagataacct tgcagcccca acaccattcc gatgaacacg ccagaaacca
    20581 caataatcag catcgacagg acgccgacat tatagagctg gcgcaccagc agcggcgcat
    20641 gtttgcgaaa ttccggtttg ccgaccagcg cattgaataa cattaacccg gcccgcccga
    20701 acgttctcag ggttttaatc cctttatgtc cgagcgacgc cagcgcattt aacagcatga
    20761 gtggcttaac tccctggtaa aagatcagcg tgataatcgc cggcaggata gcggaacgga
    20821 acaggcccgt cagctatccc gtccagaaac tgacgtacgc gcggatcagg attcgcctgc
    20881 aacgcctggg cactgccatg agcgacaatt tttttgtccg ccaggatcca ggcgtgatcc
    20941 gcaatactta acacttccgg cacatcgtga gaaaccacca cacaagtcac gcccagcgcg
    21001 ctgttcagct cagaaatcag cttcaccagt acgcccatgg tgatgggatc ttgcccaaca
    21061 aaaggttcat caaacatgat gagatccggc tccagcgcaa tcgcacgcgc cagcgctgca
    21121 cgccgcgcca tcccaccgga aagttcagaa ggcattagtt tagccgctcc acgcagcccc
    21181 acggcctcca gcttcatcat caccgtacta tgcaacaatg gcgcgggtag ttgggtatgt
    21241 tcgcgcagtg gataggcgac gttgtcaaat acgttcatat cagtgaacaa cgccccggac
    21301 tgaaataaca tgctcatccg tttgcgcact gtatacaggc gcgaacgaga catcgccgga
    21361 atattctcac catcgaaaag gatctcacca tgatctggtg cgatttgccc gccaatcaga
    21421 cggagtagcg tcgttttacc gatgcccgat ggccccatga tcgccgtgat cttccctcgc
    21481 ggcacggtca gggaaatatt atcgaagatg cagcgattgc cacgcgtaaa actgacatcg
    21541 cgcatatcga ctaaattcgc cacagactgc tccataattc acccttcgtc ttgcgttgat
    21601 tttctaagca tggcgctcaa tttaaccttg aacccaacat atttacagaa tattacccgc
    21661 cgtggttagc gaaagctggc atttgtttta ctttttagcc gcataaagtc aaaattaaga
    21721 atccgttacg gctttctgaa aatcttcagc ggaccgacga gtatacctga agaaaggacg
    21781 ttagatgctt ttagctacgg cactgttaat tgttggttta cttttggtcg tttacagtgc
    21841 cgaccgcctg gtttttgccg cgtctattct ttgccgaacc tttggcatcc cgccgctgat
    21901 catcggcatg acggtggtca gtattggtac atcgttacca gaaatcatcg tctcgcttgc
    21961 cgcgtctctg cacgaacaac gcgatttagc cgttggtaca gccctcggct caaacattat
    22021 caatattttg ctgatcctcg gtctggctgc gctggttcgt ccttttaccg tccattccga
    22081 tgttctgcgt cgtgaattac ccttaatgtt gttggtcagc gtagtggccg gttccgtact
    22141 ctatgacgga caacttagtc gcagcgatgg tatctttctc ctctttctgg ctgtgctatg
    22201 gctgctgttc attgttaaac ttgcacgtca ggctgaacgt caggggactg acagcctgac
    22261 cagagagcag cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt ttttatggct
    22321 cggcattgcg cttatcatca tgccagtggc cacgcggatg gtggttgata acgccacggt
    22381 gctggcgaat tactttgcca tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg
    22441 aaccagcctg ccggaactgg caaccgcaat agcgggggtt cgcaaaggtg aaaacgacat
    22501 tgctgtcgga aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc
    22561 cgcgctgata acgccaggag agattgagcc attggcgtac agtcgtgact acagcgtgat
    22621 gttgctggtg agcattattt ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg
    22681 tggtgtaggg gtattattaa ctggcggatt tatcgtatgg ctggcgatgt tgtactggtt
    22741 atcgccaata ctcgttgaat aactggaaac gcattatgtc gcacgtagag ttacaaccgg
    22801 gttttgactt tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg
    22861 agcttgatca atacatcaat cagaatttca cgcttgcctg tgaaaagatg ttctggtgta
    22921 aagggaaagt tgtcgtcatg gggatgggga aatcggggca catcgggcgc aaaatggccg
    22981 caacgtttgc cagcaccggt acaccttcat ttttcgtcca tcctggtgaa gccgcgcatg
    23041 gtgatttagg catggtcacc ccacaggatg tggtgattgc tatctctaac tcaggtgaat
    23101 ccagcgaaat cacggcctta attccagtgc ttaagcgtct tcacgtaccg ttaatctgca
    23161 tcaccggtcg cccggagagc agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag
    23221 tagcgaaaga agcctgtccg ttagggctgg caccgaccag cagcaccacc gccacgctgg
    23281 ttatgggcga tgccctcgct gtcgcgctgt taaaagcacg cggctttact gctgaagatt
    23341 ttgcgctctc acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata
    23401 ttatgcatac gggcgatgag atcccgcatg ttaagaaaac ggccagtctg cgtgacgcgt
    23461 tgctggaagt tacccgcaaa aatcttggta tgactgtcat ttgcgatgac aatatgatga
    23521 ttgaaggcat ctttaccgac ggtgatttac gccgtgtctt cgatatgggc gtggatgttc
    23581 gtcagttaag tattgccgat gtgatgacgc cggggggaat acgtgtgcgc cctggcattc
    23641 tggccgttga ggcactgaac ttaatgcagt cccgccatat cacctccgtg atggttgccg
    23701 atggcgacca tttactcgga gtgttacata tgcatgattt actgcgtgca ggcgtagtgt
    23761 aaagattcaa ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg
    23821 ccctgtcagc gccgacgtta tagcaaaagc agagaacatt cgtctgctga tcctcgatgt
    23881 cgatggcgta ctgtcagatg gcctgattta tatgggcaat aatggcgaag agctgaaagc
    23941 gttcaatgtt cgtgacggtt atggcattcg ttgtgcgctc acctctgata ttgaagtcgc
    24001 tatcattacc gggcgaaagg ctaaactggt agaagatcgt tgtgccacat tggggatcac
    24061 tcacttgtat caggggcagt caaacaaact gatcgccttt agcgatctgc tggaaaaact
    24121 ggcgattgcc ccggaaaatg tggcttatgt cggcgatgat ctcatcgact ggccggtaat
    24181 ggaaaaagtg ggtttaagcg tcgccgtggc cgatgcgcat ccactgttga tcccgcgcgc
    24241 cgattacgtg acgcgcattg ctggcggtcg tggcgcagtg cgcgaagttt gcgacttatt
    24301 actcctggcg cagggcaaac tggatgaagc caaagggcaa tcgatatgag taaagccaga
    24361 cgttgggtta tcattgtgct atcactggcg gttctggtga tgatcggcat tgatatggcc
    24421 gaaaaagacg ataccgccca ggtggtcgtc aacaacaatg atcccaccta taaaagcgag
    24481 catacggaca cgctcgtcta taacccagaa ggggcactaa gctatcgatt gattgctcaa
    24541 cacgttgaat attattccga tcaggccgtt tcgtggttta cgcagccggt acttaccacg
    24601 tttgataagg ataaaatccc gacatggtcc gtaaaagcag ataaagccaa gctgaccaat
    24661 gaccggatgc tctatttata tggacacgtt gaagtcaacg cacttgtgcc aaactctcaa
    24721 cttcgcagaa tcacgacgga taacgcgcag atcaatctgg tgacgcagga tgttacctct
    24781 gaagacctcg tcacgttata cggaacaaca tttaactcca gcggtctgaa aatgcgcggc
    24841 aacttacgca gcaagaacgc cgagctgatt gaaaaggtta gaacatccta tgaaattcaa
    24901 aacaaacaaa ctcagcctta atcttgtgct tgccagctca cttctggccg ccagcattcc
    24961 ggcatttgcc gtaaccggag acactgatca gccgatccac attgaatcgg accagcaatc
    25021 tcttgatatg caaggcaacg tggttacctt taccggtaat gtcatcgtca cccagggcac
    25081 catcaaaatt aatgccgaca aagtggtcgt tacccgtccg ggcggcgaac aaggtaaaga
    25141 agtgattgac ggctacggta aaccggcaac gttctaccag atgcaggaca acggtaaacc
    25201 cgttgaaggt cacgcttccc agatgcacta cgaactggca aaagattttg tcgttctgac
    25261 gggtaatgct tatctgcagc aggtcgatag caacattaag ggcgataaga tcacttacct
    25321 ggtaaaagag cagaaaatgc aggctttcag cgacaaaggc aagcgcgtaa ccaccgttct
    25381 ggtgccgtcg cagctgcagg acaaaaacaa caaaggccag accccggcac agaagaaggg
    25441 taattaattc gttatggcaa cattaactgc aaagaacctt gcaaaagcct ataaaggccg
    25501 tcgcgtggta gaagacgtca gcctgaccgt caactccggg gaaattgtcg gtctgctggg
    25561 gccaaacggt gccggtaaga ccaccacttt ctacatggtt gtaggcattg tgccgcgcga
    25621 tgcgggcaac atcattattg atgatgacga tatcagtctg ctgcctctgc atgcacgcgc
    25681 gcgccgcggt atcggctatc tgccacagga agcctccatt ttccgtcgcc tcagcgttta
    25741 cgataacctg atggcggtac tgcaaattcg tgacgacttg tctgctgaac aacgtgaaga
    25801 ccgcgcgaac gagctgatgg aagagtttca cattgagcac ctgcgtgaca gcatggggca
    25861 gtcactctcc gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc
    25921 gaaatttatt ctgctcgacg aaccgtttgc cggggttgac ccgatctcgg ttatcgacat
    25981 taaacgcatc attgagcacc tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa
    26041 cgtgcgtgaa acactggcgg tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat
    26101 cgcccacggc acgcctacag aaatcttaca agacgaacac gttaagcgtg tataccttgg
    26161 ggaagacttc agactctgat agggtagaag tttgcgacgt tttagcagga gagtacgatt
    26221 ctgaacatga agcaaggttt gcaactcagg cttagccaac aactggcgat gacgccacag
    26281 ctccaacagg caattcgtct gttgcagttg tcgacgctgg aacttcagca ggagctacag
    26341 caggcgctgg agagtaatcc gctgcttgag caaatcgaca ctcatgaaga aatcgacacc
    26401 cgcgaaacgc aagacagtga aacgctggac accgccgacg cgctcgaaca aaaagagatg
    26461 ccggaagagc tgccgctcga tgccagttgg gacaccattt acaccgctgg tacaccatcc
    26521 ggcaccagcg gtgactacat tgacgatgag ctgccggtct atcagggcga aacgacgcag
    26581 accttgcagg attacctgat gtggcaggtc gagctgacac cgttttccga cactgaccgc
    26641 gctattgcta cctctatcgt cgatgccgtt gatgacatcg gttatctgac tgtcccgctg
    26701 gaagatattc tcgaaagtat gggcgatgaa gagatcgaca tcgacgaggt tgaagccgtc
    26761 cttaagcgga tccaacggtt tgatccggtc ggtgtagcgg caaaagatct gcgtgactgc
    26821 ctgctaatcc aactctccca attcgataaa accacgccgt ggctggaaga ggccagactg
    26881 atcattagcg atcatctcga tctgttagcc aatcacgact tccgcacttt aatgcgcgtc
    26941 acgcgtctga aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg
    27001 cgccccgggc agtcgatcca gactggcgaa cctgagtatg tcattccaga tgtgctggtg
    27061 cgtaagcata acggtcactg gacggtagaa ctcaacagtg acagcattcc gcgtctgcaa
    27121 atcaaccagc actacgcctc gatgtgcaat aacgcgcgca acgatggtga cagccagttt
    27181 atccgcagca atctgcagga tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat
    27241 acgctactgc gcgtgagtcg ctgtatcgtt gaacagcagc aagccttctt tgagcaaggt
    27301 gaagaatata tgaaaccgat ggtactggcc gatatcgccc aggctgtcga aatgcatgaa
    27361 tcgacgatat ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg catttttgaa
    27421 ctgaagtatt tcttttccag tcacatcaat accgagggcg gcggcgaagc ttcctccacg
    27481 gcgattcgtg cgctggtgaa gaaattaatc gcggcggaaa acccagcgaa accgttgagc
    27541 gacagcaagt taacctcttt gctgtcggaa caaggtatca tggtggcacg ccgcactgtt
    27601 gcgaagtacc gagagtcttt atccattccg ccgtcaaacc agcgtaaaca gctcgtttga
    27661 cccaaccgat aaggaagaca ctatgcagct caacattacc ggaaataacg tcgagatcac
    27721 cgaggcactg cgcgaatttg ttacagccaa atttgccaaa cttgagcaat attttgaccg
    27781 aatcaaccag gtctatgttg ttctgaaagt ggagaaagtc acccacacct cagatgcaac
    27841 actgcatgta aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc
    27901 cattgatggc ttaattgata agctggcacg ccagttgacc aaacataaag ataaactgaa
    27961 acaacactaa ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg
    28021 gccatttgta cggttaatgc tccgagcctg ttccactgtt tgagtgggca ggttcttagg
    28081 tgaaattatg acaaataatg atacaactct acagcttagc agtgttctta acagggaatg
    28141 tacgcgaagc cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct
    28201 ggcggcgaaa caacttagcc tgccgcctca ggtggttttt gaagctatcc tgacgcggga
    28261 aaaaatgggc agtaccggta tcggcaatgg tattgccatt ccgcatggca aactggaaga
    28321 agatactctg cgcgccgttg gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc
    28381 catcgacaac caaccggtgg acttactttt tgccctgctg gtgtcggcag accaaactaa
    28441 aacgcacctg catactctgt cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg
    28501 ccgtttacgc gcagcccaga gcgatgaaga gctgtatcaa atcattacgg ataccgaagg
    28561 tactccggat gaagcgtagt tattcggtaa tgtctctttt agacgttgtg aggagaaaca
    28621 gtacatggta ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg
    28681 tgcgctggaa gatatgggtt tttactgcgt ggataacctt cccgtagtgt tgttacccga
    28741 tctggctcga accctggccg atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa
    28801 tatgccggag tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg acgctttctc
    28861 accgcaactc ctgttcctgg atgccgaccg taatacctta attcgtcgtt acagtgacac
    28921 gcgccgactg catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga
    28981 aagcgatttg ctggagcctc tgcgttcgcg agcggatctg attgtcgata cctcagaaat
    29041 gtccgttcac gagctggctg aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga
    29101 actgaccatg gtctttgagt ctttcggctt caaacacggt atccctatcg atgcagatta
    29161 cgtctttgac gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc gtccaatgac
    29221 aggtcttgat aaacctgtcg ccgcgttcct cgaccgccac acagaagtac acaattttat
    29281 ctaccagacg cgaagctatc ttgagctatg gttacctatg ctggaaacca acaaccgtag
    29341 ctacttgacg gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt atattgcaga
    29401 gcaactggca gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct
    29461 ggaaaaacgt aaaccatgac cgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg
    29521 catgcccggc ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg
    29581 ttacgtaatg acgaaggcac cgaggccgaa gccaacagcg ttattgcgct gctgatgttg
    29641 gattctgcca aaggacggca gattgaagtt gaagcgaccg gcccacagga agaggaagca
    29701 ctggccgccg ttatcgccct ctttaactct ggttttgatg aagattaatc ttcatcactt
    29761 tgacatacaa ctcccttcaa actccccccg acaataagaa aatcacgtac tgaaatcgtt
    29821 ctcaatcaac gtcatttgta cattttgtgc gcttttcact ttcagaagaa ccttaagaaa
    29881 accttaagag gcattgttta ggttttgttt aagttaatcg accatactgg agatcgtcag
    29941 aaaatatttc caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga
    30001 agacatcgga aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac
    30061 catcgtggcg atggtggcga taaagagatc cgccgtgagt taaagtacta cgcgcatctt
    30121 ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat
    30181 tgcggaaccg gttatgtcta cgatgtgatt gcagattttg acggcaaact ctccattacg
    30241 ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact gcgccagcta
    30301 ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg
    30361 cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt ctgcgacaac
    30421 attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg tctgcgtaag
    30481 caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa
    30541 gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc
    30601 tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc
    30661 tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg attaggtaat
    30721 acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt gtggaaataa
    30781 cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa
    30841 accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa tccagcttcc
    30901 agcccttttc gtacccagct acgcccatcc cataaaaaga gatttttggc cgtctgctga
    30961 gaaatcgttg aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggctttc
    31021 tcaatggaag cgacatcaaa gccccagtgc tcagaaaatt tctgatcttc tgcggcaatc
    31081 accgccagtc ccatccacgg cgagatttga tccatactga cccagtcaga atgtgccacg
    31141 taacgaaaat tgccatgcag ccaggcgctg acctgtcgct cgaccattac cgctgagaag
    31201 ggaacaggcg caacactaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc
    31261 attaacagca aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt
    31321 cagccagaac cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt
    31381 tctgcgccag catatatgct ggggtggtga caatcttatt gtcttcatca accacgatat
    31441 catcgacagg acagggcaca tgctccgcgc ccatctcttc cagcacttct gcggtatcga
    31501 tatcagtacc gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg
    31561 caatacacat aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta
    31621 attcacggtc aacggtgcat tcgctaccaa gagtggcaaa attgcttaaa ttcttcgccg
    31681 cgccaaaccc ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag
    31741 gacggatttc accgcgcgtt attcgtgccg cttcaatcag cacgttgcgc gtttccgtca
    31801 tcgcttcgcc agttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga
    31861 ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag
    31921 aaccgtcata gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcatcc
    31981 tttcgcaatt gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt
    32041 aactgattca tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa
    32101 acagtgctgt gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg
    32161 tgttggcgca gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc
    32221 acccacgctt tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat
    32281 tcaccgacgt tatcttccca ggcaggaagg tcaggagact gaatttgctg acccagctgt
    32341 tgcagatggc gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca
    32401 atgccttttt tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc
    32461 atcttctcaa acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac
    32521 tgttccagca tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc
    32581 gtactctcct catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga
    32641 acagaaagcg gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct
    32701 ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat cctctcgcgg ataacgcttc
    32761 gtcagttcac gagagatatc cagcccggtc atatctggca actgaatatc cagtaacacc
    32821 aggtcgtatt cgcccggttt aaacatctct agcgccgcct tgccggtcat ggcgacatca
    32881 acgctgttac ctaatttttc cagcacagaa cgcgcaacaa tcacgttcag ttcaatgtct
    32941 tccaccagca gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg
    33001 acctcttctg ctaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt
    33061 tcgctggtaa ccgtaatatc gccgcccata tttttcgcca gacgacgaga aacggccaga
    33121 ccaataccgg tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg
    33181 gcgaaaattt tatccagctc atcctgcgga atgccgatac cagagtcttc cacttcaaaa
    33241 tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat
    33301 ttgacggcgt tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg
    33361 acctgatgcg gtaatggcag cgtcggctcc aggttaaagc gcaatccttt ttgttgcgcc
    33421 tgcaatgcgg agagattttc cagatcggca aggaagctgg tgaaatcaac cggttgatta
    33481 tcaagctgga ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc
    33541 cccagcgtga cggccgaaac atggatggtc ttgagatatt tttcctgctc ggcggtgagt
    33601 tcggtatcca gcagaatgcg gctcagaccg acgataccgt tcagcggtgt acgcaattcg
    33661 tgactgatgg tggagataaa cgtcgttttg tcgcggctgg cccgttcaag cgcatcctga
    33721 taccgcttac gctcggtaat gtctcgacca aagcccatca aaccgtgacg tttacccacg
    33781 cggtcgtagt acggcacttt acggatttca aagcaggctt tgcgcccgtc cgggtaatcc
    33841 agccactgtt cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg
    33901 acttttgcgg cggcttccgg tgagtaaaca tcaacaggtt tcaggtgaac cagttgtttt
    33961 tcgctttttc cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg
    34021 ttacgataaa aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa tgaggattgc
    34081 tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag
    34141 gtttcctgca gttctgcttc cgctttttca cgaacggcga tttcctgatt tagctgggca
    34201 atattatctt ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt
    34261 tgcaccagcc gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga
    34321 aagtagaccg cccacggcgt aatcaacaaa ccaaagaaga tagaacgaat aacatcaatg
    34381 ctttcgacct gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg
    34441 acgagggcca gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca
    34501 acataatact gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg
    34561 ccccaataat acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc
    34621 cctcaacgac ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc
    34681 catgttcatt cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca
    34741 gaggagcaag cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg
    34801 gtggcgtatg gcgaatcatt tctccggcag tgaacgtgta atcctccagt tcaataccgt
    34861 tcaaacgccc cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcaacggat
    34921 gtagctttat accgtcaaca ccggtttcca ctacccgctc cagcgtttgc agacattcag
    34981 ccagcccttc gcctggcaga ccgacaatta aatgcgagca caccttcagc ccgcgctggc
    35041 gtgccagctg cgtcgtgcgc tgataacagg cgaaatcatg accgcgatta atgcgatgta
    35101 gcgttttgtc gtgcacggtt tgcagcccca gctccagcca cacttcgtag ccctggtctt
    35161 tatattcgca aagcagatcc agcaccgcat ccggcacgca gtccgggcgc gtgccgacac
    35221 acaaaccgac aatattggcc tggctcaccg cctgttgata catcgaacgc agcacctgaa
    35281 cttcggcaaa ggtgctggta tacgcctgga aataggcgag gtaacgtttc gcgcgattaa
    35341 ttaaattcgc ctgatgcgcc agctgttcgg caatggaacg atgctgctgc gcttcgtcgg
    35401 caaacgaggc aacattacag aaggtgcagc cgccgcgccc gatggtgccg tcgcgattcg
    35461 ggcaactgaa accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga
    35521 gatcaccacc aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt
    35581 gaaaagaggc caaagcctgc catttttagc tcaattcggc gatgacctgg atcaatcgtc
    35641 ccggcccgct tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcataatca
    35701 cataaatcac ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca
    35761 aaaacagtag catgaaacgt cattaccaat taatgcagta taaaatgctg gttttctcgt
    35821 caattcaagg caggataagg gttaacacgc ctttatgaca gtcaggaatt gactgtttct
    35881 ctaacgactt cccttttagc cttaaagata aaatctattt taatttcagc catttaataa
    35941 agaattttgc gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc
    36001 tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat
    36061 ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg
    36121 gggcgaatgc gaggcgcgcg tatgacacgc aacccccgtc gccacgctct ttctgtaccc
    36181 gtgcgcagcg gttcggtagt ggggttcccg cagagcctgg gggaggttca cgatatgttg
    36241 tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc
    36301 gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt
    36361 ggcgcgattc tcgccgatgg taaaaccggt gacggttgcg gcttgctgtt acaaaaaccg
    36421 gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcgaa aaactacgcc
    36481 gtcgggatgc tattcctgaa taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt
    36541 gaagaagaac tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt ccccactaac
    36601 gaaggcgtac tgggtgaaat cgccctctcc tctctgcccc gcattgagca aatttttgtg
    36661 aacgccccgg caggctggcg tccgcgcgat atggagcgcc gtctgtttat cgcccgccgc
    36721 cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct atcgaatctg
    36781 gtgaacatct ataaaggtct gtgtatgccg gcggatctgc cgcgctttta tctggatctt
    36841 gcggacctgc gtctggaatc ggccatttgc ctgttccacc agcgcttctc cactaacacc
    36901 gtaccgcgct ggccgctggc gcaaccgttc cgctatctgg cgcataacgg tgaaatcaac
    36961 accatcactg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca gacgccgctt
    37021 atccctgatc tgcacgacgc cgcaccgttc gtcaacgaaa ccggctctga ctccagttcg
    37081 atggataaca tgctggaact cctgctggca ggcgggatgg atatcatccg cgccatgcgt
    37141 ctgttagtgc cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc
    37201 tttgacttta actccatgca tatggagccg tgggatggcc cggcgggcat cgtgatgtcc
    37261 gacggtcgtt ttgccgcctg taacctcgac cgtaacggtc tgcgtccggc gcgctacgtc
    37321 atcaccaaag ataagctcat cacctgcgcc tctgaagtcg gtatctggga ttaccagcct
    37381 gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggttat cgacacccgc
    37441 agtgggcata ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccatccatat
    37501 aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa
    37561 gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa acagtttaac
    37621 tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg
    37681 gtcggttcga tgggcgatga taccccattc gccgtgctct ccagtcagcc gcgcattatt
    37741 tacgactact tccgccagca gtttgcccag gtgactaacc cgccaatcga cccgctgcgt
    37801 gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt cttttgcgaa
    37861 gcagagggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ctccgatttc
    37921 aaacagctca cgacgatgaa agaggagcac taccgcgcag atacgctgga tatcaccttt
    37981 gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc tgtgcgacaa agccgaaaaa
    38041 atggtacgta gcggcaccgt gctgctggtg ctctccgacc ggaatatcgc taaagatcgc
    38101 ctgccggttc cagccccgat ggcggttggc gcgatccaga cccgtctggt cgatcaaagc
    38161 ctgcgttgcg atgccaacat catcgtcgaa accgccagcg cccgcgatcc gcaccacttc
    38221 gccgtgttgc tgggcttcgg cgcgacggct atttatccat accttgccta tgaaacgctg
    38281 ggccgcctgg tagacaccca tgcgattgcc aaagattatc gtaccgtgat gctcaactac
    38341 cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc
    38401 gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc tacacgatga tgtagtgggc
    38461 ctgtgcttcc agggggcggt cagccgcatt ggtggggcaa gctttgaaga cttccagcag
    38521 gatctgctga atctgtcgaa acgtgcctgg ctggcgcgta agcccatcag ccagggcggc
    38581 ctgctgaaat acgtccacgg cggcgaatac cacgcctaca acccggacgt ggtgcgcacg
    38641 ctgcaacaag cggtacaaag cggcgagtac agcgactatc aggaatacgc gaagctggtt
    38701 aatgagcgtc cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg
    38761 gtcaacattg ctgatgttga accggcaagc gaactgttta aacgcttcga taccgctgcg
    38821 atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaacagc
    38881 atcggcggta actcaaactc cggtgaaggc ggtgaagatc cggcgcgcta cggcaccaac
    38941 aaagtgtcgc gcatcaagca ggtggcttcc ggtcgctttg gggttactcc ggcgtatctg
    39001 gtcaatgccg acgtcattca gattaaagtc gcccagggcg cgaagccggg cgaaggcggt
    39061 cagttgccgg gtgataaagt cactccttac atcgccaaac tgcgctattc ggtgcccgga
    39121 gtgacgctga tctccccgcc gccgcaccac gatatctact ctatcgagga cttagcgcag
    39181 ctcattttcg acctcaagca ggttaacccg aaagcgatga tctccgtgaa gctggtttcc
    39241 gaaccgggcg taggcaccat cgcgactggc gtggcaaaag cttatgccga tttaatcacc
    39301 atcgcaggct atgacggtgg caccggcgca agcccgctgt cgtcagtgaa atacgcaggt
    39361 tgcccgtggg agctggggct tgttgaaacc cagcaggcgc tggttgccaa tggtctgcgt
    39421 cataagatcc gtttgcaggt cgatggcggc ctgaaaaccg gtgttgatat catcaaagcg
    39481 gcgattctcg gcgcagaaag cttcggcttc ggcactggcc caatggtcgc gctcggctgt
    39541 aaatatctgc gtatttgcca cctgaacaac tgcgcaacgg gtgtggcaac tcaggatgac
    39601 aaactgcgta agaatcacta tcacggcctg ccgttcaagg tgacgaatta ctttgagttt
    39661 atcgcccgtg aaacccgcga gctgatggca cagctggggg taacgcggct ggtggatctg
    39721 attggtcgca ccgacctgct gaaagagctg gacggtttca ccgccaaaca gcagaaactg
    39781 gcgctgtcga agctgctgga gaccgccgaa ccgcatccag gtaaggcact ctactgcacc
    39841 gaaaacaacc cgccgtttga taacggcctg ctgaacgcgc agttgctgca acaggcgaaa
    39901 ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atatccgcaa taccgatcgc
    39961 tccgtgggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggctggca
    40021 gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagtttcgg tgtatggaac
    40081 gcgggcggcg tggaactgta cctgaccggg gatgccaacg actatgtcgg taaaggcatg
    40141 gcgggcggct taatcgccat tcgtcctccg gttggttccg ccttccgcag ccatgaagca
    40201 agtattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc
    40261 cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt agaaggcatt
    40321 ggcgacaacg gctgtgaata tatgacgggc ggtattgtct gtattctggg taaaaccggc
    40381 gttaacttcg gtgcgggcat gaccggcggc ttcgcttacg ttctcgatga aagcggtgat
    40441 ttccgcaaac gcgttaaccc ggaactggtc gaggtcttaa gcgttgacga tctggcgatc
    40501 catgaagagc atctgcgcgg tcttatcacc gagcatgttc agcatactgg ctctcagcgc
    40561 ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacta aatttgcgct ggttaaaccg
    40621 aagtccagtg atgtaaaagc actgctgggt catcgtagtc gtagcgcagc agagttgcgc
    40681 gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg
    40741 cagcgcgttg atccgccaaa gaaaccgctg aagatccgca aaattgagtt tgttgaaatt
    40801 tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgcct gtcgtgcggc
    40861 aacccatact gcgagtggaa atgtccggta cacaactaca tcccgaactg gctgaagctc
    40921 gccaacgagg ggcgtatttt tgaagcggcg gaactgtcgc accagaccaa caccctgccg
    40981 gaagtttgcg gacgagtctg cccgcaagac cgtctgtgcg aaggttcctg cactctgaac
    41041 gatgagtttg gcgcggtgac catcggcaac attgagcgtt atatcaatga taaagcgttc
    41101 gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa agtggcgatt
    41161 atcggcgcag gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa cggcgtaaaa
    41221 gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg tattccggcc
    41281 ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg catgggtatt
    41341 gaattcaaac tcaataccga agtgggccgc gacgtgcagc tggacgatct gctgagtgat
    41401 tacgatgccg tcttccttgg cgtcgggact tatcagtcaa tgcgcggcgg gctggaaaac
    41461 gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta
    41521 atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggtg
    41581 gtccttggcg gtggcgacac tgcgatggac tgcgtgcgta cgtccgtgcg ccagggagcg
    41641 aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa
    41701 gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc actggggatt
    41761 gaagtgaacg gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga aatgggcgaa
    41821 ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca tatcgttccg
    41881 gcagatgcag tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa
    41941 cacagcgtcg agctggattc acaaggccgc atcatcgccc cggaaggcag cgacaacgcc
    42001 ttccagacca gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg ttccgatctg
    42061 gtggtaaccg ctattgccga aggtcgtaag gcggcagacg gtattatgaa ctggctggaa
    42121 gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt
    42181 ctttctggaa agcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt
    42241 gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg ccatataaag
    42301 ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca aatccacgac
    42361 ggtattattc acctggtcga tagcgcccgg acggaaacgg tacgtagcgt taacgcgctg
    42421 atgaccgcga catactggga aattggccga cgaattgtcg aatttgaaca aggtggcgag
    42481 gcaagggctg cgtatggtgc gcagctaatc aagcgactat caaaggattt aagtctaagg
    42541 tataagcgtg gtttctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt
    42601 caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg aattccgcag
    42661 acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc caaattgaga
    42721 ggctgtttga tatttccaca gcttttgtat tgcatctcct attttcggtt tgtatattca
    42781 tgcaaataaa cgaaggaaag ttgccgtgat ttacattgaa cagcttgaat taatccataa
    42841 aagtggtgat gtgttgtatc cagtaaaaat aaccagaaaa tcatcaggaa aaactgcctt
    42901 ccaccttgtt ccattcggct taaataagac tcacgacctt ctagaggtgg aagatgcttc
    42961 agaagcaatt cggctggtga ttgatgaacg tcattccatt cgctgcagta cacttacagc
    43021 aactattacg aataaaaagg gcaagcgaat taagcggaca ggtatataca gtatcaaggg
    43081 tgttaacata aaagaataca acgtcaggta atggcacaga gcggtccgtc agttcaggtt
    43141 tggctctgtg ccaaagatgg gtcaggtcac acctgatcta ataaatttaa gccattaaat
    43201 cagccttaac cttcaccacc acctttttaa tctcacccgg ctcgccaaca acgcatcccg
    43261 gcttatgcgg ctctcctagc ataaacacgg cgaacattcc cggtttcagg atgatggctt
    43321 gctcgttttc aatggtgctg caaagctggt aatcatcctc atggtggaac tcttcacact
    43381 gacgcgcagt gcctgccatg ccaaacagaa tccgttcttc accgtttaat aacagctgga
    43441 tatcaatgta ttgctcgtgc agctccgctt ttttctcaac gggcgattga gtgttaaacg
    43501 tcatgacatt cataaaaata ttgtcgccct gtaattcgta acgacccggc gctttttctt
    43561 gcggtctggc agctaatgcc agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag
    43621 acggtaatga ccgtacttca cccatcatca taatttttct ccctgggcca acagcgctgc
    43681 cccaagtaaa cctgcatcat ggcggtagtg cgccgccagt aaatcaacat gaaataccgc
    43741 tggctcctgc gccagatacg tttccaccaa cgccagatac ccttctgcca gaccaacgct
    43801 gccaccgacc accacgcact ggcaatcagt tgtggcttta atatcagcga tcagccttgc
    43861 cagcacatgt gcggagcggt gaatcagttg ctgcgcctgc tcgtcgccct gcccggcgcg
    43921 tgtgaaaata gttctcgcat ccgcgcccat gagttcacct tgcgctgccg ctgcaatacc
    43981 gcgaccagaa gcaatcgctt ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg
    44041 tggatcggca agcgtatgcc cgatatgccc cgccagaccg ccagggccgg taagcagttt
    44101 accgccgctc actacaccgc cgccgacgcc agtggaaacg gtgataaaca ccatctcggt
    44161 tacatcgcct tccagcgcct gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc
    44221 aatggtcggc aaattggtaa gttgttccag cgttttgact aacggaaagt gtagcaatcc
    44281 accgagatta tgcggattca gcgccagcaa actgccgtcg cgaataattc cggttgaagc
    44341 gatggcaacc cgctgcgcat gagcttgcaa cggagagact aatgcggata aggcatcacg
    44401 caaggcttct ggtgtctggc tggctggcgt aggaagttca cgacgatcgc ggatctgccc
    44461 gtcagcgcca atcagcgcgg cggcaagttt agtaccgccg atatcaatcg ccagtgtggt
    44521 catagcaccg cctttttcat cgctgtgttg taccactgac aaatgtgttc aagacgcgta
    44581 atggcagaac cgaccgtcac cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca
    44641 ggcgtgttgt aacgcccttc ggcaatcacc cgacatccgg cttcgctcaa cgttttcacc
    44701 agcgccagat ccggctcttc tggcgtttca ggcgtggtat agccagaaag cgtagtgcca
    44761 ataatttcgg ctcccagctt ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc
    44821 atcgccagta aaccgtgatg atgtatacgt gccagcagcg tttcaacagg caccggacgc
    44881 gggcggtcgg tgccgtcaat ggcgataatg tccgcgcccg cctgcgccag cgcatcgaca
    44941 tcttcaatat aggccgtgat gcgtaccgga gaatcctcca gatcgcgttt cacaattcca
    45001 ataatcggca cgctcaccac cgcacgcgtg gcttgcagat ttgccacacc ttcgatgcga
    45061 atggcaaccg cgcccgcctg ctctgccgct aatgccatgg ctgcgacgat ttcgggttta
    45121 tcgagcgggc tgtcaggaat cggctggcag gagacaatca ggccaccgtt agcagcgatt
    45181 ttttgatcca gttgtgcaag taacgacata catcttccct tagcgaaagg cccggtacat
    45241 agaccgggca acaggattaa cttttggttt tgactaaatc gtttttggcg ctgccaaatg
    45301 gcacggcacc gctaaatggt ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc
    45361 gcagccaacg ctgaacgcgg gaaggcatat ccagaccaat cagcaggatc accacaaatg
    45421 tcaggctgaa cgagagcgat gccagcgcag tacccagatc cagacgttga gcgatcaacg
    45481 cgccgatgat tggggccagt gcgccaccca atgcgccaac gttgtaggta aagcccaggc
    45541 ccgctgcacg ctggtcggta tcgaaataac cgccaatcag tttcggtaag atcccggcga
    45601 tcccttgtcc aagcatttgc tggaagaaca gtaacagacc gagcacccag acgtttgcgc
    45661 cgccaatcgc aaataccgga ataatcagca gctgcgaggc cagcaggcta caaacgtacg
    45721 ctttgcgggt tcccagccag tcaccgagga agcctcctac gcagcatccc acggccgcgc
    45781 caaagccact aaagaacagc acattggcga cagtatgcgg gttataagcc agatcggttt
    45841 tcagatacgt tggcagcagc gcctgaatcg gccatgagta gaggaaagca aacaacacga
    45901 ccaccatcag cattacgccc gttggccagc gtttgcctgt actctgcacc ataaagctga
    45961 taaagattgc ggcgcataac agcccaagaa cagcgacgat cgcggcattt tgcaggttac
    46021 cggcgaagca gaaccacagc gcagtagccg ccgccagtgt cattacgata ttggcaatgc
    46081 gatgttcacc acggtagaga atatccacca ttgtgcgtac tggtgcttta cctgcgtgtt
    46141 tctctttcca gtcttccgct tccgggatgt ttttacgcag ccagagagca aagatgattg
    46201 gcaaaatgcc gataaagaac agcgcacgcc aaccccagac cggaaccacc aggctataga
    46261 cctgagcggc aacgacggcc cccacagaga agcctgaaat caaaaaacca ctggctttgt
    46321 tacgcagatg ttttggccag ctttcaatga cataggtggc gctggaaccg tattcacccg
    46381 ccatccccat gccgatgacc agacgagcga taaacatggt gatgtagcct ggcgcaaagc
    46441 cgcaggccag cgtcccggcc gagaagagaa cgatgctggt gaccattgcc agacgacgcc
    46501 cgtagcggtc acccatagcg ccgagcatca ggccgccgaa ccagcgagag ataaaggctg
    46561 cggagatcag acttgccgcc tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga
    46621 gtaccagggc gattaaaacg aaatcaaaac cgtcaagcag atatcccaac caggcagcgg
    46681 aaaatgcgcg ccattgtgca cggttgagat ggcgatacca cgggatattc tgggttgtag
    46741 tactcattgt gagtctcccg cggtgggcga tgcccacacg ctttggtatg aaaattgtag
    46801 ggtacagatg cgtttatttc ccctcacccg gtaggggcga gcgaggggaa aacaactcac
    46861 ccgcgctctt gcatcaactg ctgggccagc gccttcagtt ctggcagata tttttcatct
    46921 accggtccaa acggtttgcg gcacagcggc acagaaacga catccatata atgcaggaca
    46981 gttttcaggc cgcggaatac gccagttttg atcagtaaat caatgacttt gttgcactca
    47041 gtttgcagtt tctgcgcggt ctggatatcg ccttctttca gcgccttcac gatcccctga
    47101 tatcgccagc ccatgatgtt gtaagtactg ccgataccac catcagcgcc tgccagcaag
    47161 ccggaagcga agatttcgtc gtaaccgtta tagagcacca gatcaggatg ttcacgacgg
    47221 atctgttcca tctgatagag atcgccagaa gtctgtttca gcgcaccgac gccaggcaac
    47281 gtaaccaggg tgttgatctg atccagggtc agttttaccc cactcagggc tggaatgttg
    47341 tacacaacca tcggcaaacc atccgctgaa tcaataattg cccgatagtg atcgcagtgt
    47401 tcttcaaagc tgaaaggata gtagaacggc gtgacggcgg agacggcatc gaagccataa
    47461 cgtttagccg atgccgcaag ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg
    47521 atgagtttaa tcttaccttt cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg
    47581 gaaaggcttt gtacaaaggc ctcgccggtc gaaccaccca cgtataaacc gtcgatgccc
    47641 tgctgaatat tgaactgaac caggcgacgc agactcgctt tatccagtgc ttgttgttgg
    47701 tcaaaaggag tcaggagtgc agccattacg ccacgtaaat tcgttgccat aaatacctct
    47761 gaagtgatac ttgtctgata aacgatatac ctttatacct gttataccag atcaattaag
    47821 caacacccca tacagaaagc ttataatgcg atctgcttca ctaaagtggc attatttctt
    47881 tttgttggtg gtctgaccga aagcgtgcca ggtagcagag acgctgttga gatgcgattg
    47941 caacgcacga tcggcttcgt caggatcatg acggcggatc gcatcaacga tcgcaatatg
    48001 ctgttgatag ctaacgttgt tatgctcgtg cagagcctga tcagccacgg ttggacgtgc
    48061 ggcgatcagc cagtcgagca gggcaacgtg gatcgccatg aagattgggt taccagggat
    48121 ctccgccagc acgcggtgga aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag
    48181 cgactgactg ttgatttcca gtgcttttgc cagcaaatcg atttgctcat cggtggcatg
    48241 ttcagccgca tagcgcacca gactggattc aaagaacaga cgtaattgtt cgaaatgggc
    48301 aatcccaccg ggatgagaaa ggaaatcttt cgccatgccg gaaaactcac cgatgatagt
    48361 gtccgcagaa ggacgcgaga cgcgagcgcg ttcgccgttg tttatttgca ccagaccttt
    48421 gcgttttaac gctgccagcg cttcgcgcac agaaggacgc ccgacgttaa agaacgccat
    48481 cagttcgcgt tcagacggta attgttcacc ttcgccaaat tcacgacggc ggatcatctg
    48541 ttccagctct tcttccacca tttcggagag ttttttacgc gccagcgggc ggctacgcaa
    48601 gttgcgacca attgcaggtg aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc
    48661 cattctgtaa ggtcagtgtg attaacatca tcagtgacat cctatcacag gattgaaagt
    48721 aggggaaaat ggcaggtttt tctctttgtg cctcatcatt accataatta acggaataat
    48781 taactattgc gaaaaattaa tgtaacgcag ataaacacat cccgattgaa ttattcataa
    48841 gactattcac aagcattatg aatattatga atgtgttctt acaaaataat cataagcgca
    48901 tattttttaa tgaaaaatca catcacctac aattaaaaac acgacatccg caccataaat
    48961 agacttgcaa aaaatataac atcattgttt tcaatctgcc gtttatggga ttgaccgttt
    49021 tcttttgaca cggagttcaa caatgttcgg cataattata tctgtcatcg tattaattac
    49081 gatgggctat ttgatcctga aaaactacaa acctcaggtg gtgctggctg ccgcaggtgt
    49141 cttcctgatg atgtgcggag tctggttagg gttcggtggt gtactcgatc ccgccaaaag
    49201 cagcggctac ttgatcgtcg acatttataa tgaaatcctg cgcatgctgt ccaaccgcat
    49261 tgccggattg gggctgtcga ttatggcggt gggcggttat gcccgctaca tggagcgcat
    49321 aggagccagt cgcgcgatgg tgagcttgct aagccgcccg ttaaaactca ttcgctcgcc
    49381 gtatattatt ctgtcggcaa cttacgtcat cggccaaatc atggcgcagt ttattaccag
    49441 cgcctccggt ctgggtatgt tgctgatggt caccttattt ccgacgctgg tgagtctggg
    49501 agtaagtcgc ctctctgcgg tggcggttat cgcaaccacg atgtccattg agtgggggat
    49561 tctggaaacg aactccattt ttgcagccca ggtcgcggga atgaaaattg ccacatactt
    49621 cttccactac cagcttccgg tcgcctcttg cgtcattatc tcggtggcga tctcccactt
    49681 tttcgtgcaa cgcgcttttg acaaaaaaga taaaaatatc aatcacgaac aggcagagca
    49741 aaaaactctc gataatgtcc cgccgctcta ttacgccatt ttacctgtga tgccgttaat
    49801 cctgatgctc ggctcgctgt tcctcgccca cgtcgggctg atgaagtcag aactgcatct
    49861 ggtggtggtg atgttactaa gtttgacggt gacgatgttt gttgagttct tccgcaagca
    49921 taacttgcgc gaaacaatgg acgatgtgca ggcgtttttt gacggcatgg gtacgcagtt
    49981 tgccaacgtg gtaacgctgg tggtcgcggg tgaaatattt gcgaaaggct taacgacgat
    50041 tggcactgtt gatgcggtta tcaggggtgc ggagcattct ggtctgggcg gtattggcgt
    50101 gatgattatt atggcgctag tcattgccat ttgtgccatt gtgatgggct ctggcaatgc
    50161 gccgtttatg tcatttgcca gtcttattcc gaatatcgca gccggactac atgtaccagc
    50221 ggttgtaatg attatgccga tgcattttgc cacgacgcta gcgcgcgcgg tttcgccgat
    50281 tactgcggtg gtgaagcgga cagcgatccc catggcagtc ggtttcgtgg tgaatatgat
    50341 tgccacaatc acgctatttt attaagtcat taaaaagaca aaacaggccg cctgggcctg
    50401 ttttgtatta cttcacaaca cgtaatgccg gtcgaccacc gcgtggtggc tgcggaggtt
    50461 catcgtcagg atgagtgtca tcatcgtgat ctggcttgtc gccatcaata accgacataa
    50521 cggtttcgtt gtctgccgat gcctcttcat cattcatgat gctggtatct tcatcgtagg
    50581 cagcttcagg ctcaaacatc gtgcctgcgc cattttcacg ggcgtagata gccagcacgg
    50641 cagccagcgg cacagaaacc tgacgcggaa tgccaccaaa gcgcgcgtta aagcgcacct
    50701 catcattcgc cagttccaga ttgccgacag cacgcggcgc aatgttgagt acgatttgcc
    50761 cgtcacgcgc atattccata ggaacctgca cgccagggag cgtcacatcc accaccaggt
    50821 gcggcgtgag ctggttatcc agcaaccact catagaatgc acgcagcaga tagggacgac
    50881 gtggtgttag ctgtgacaaa tccatacaga ttaactccgg cccagacaca tttcacgttc
    50941 tgcttcagtt aaagaagcaa ggaaagagtc acgctcaaag acgcgggtca tatagccttt
    51001 cagctctttc gcacccgggc cgctgagctc gatacccagt tgcggcagac gccacagcag
    51061 cggagcaaga tagcaatcga ccaggctgaa ctcatcgctc aggaagtacg gcttctgacc
    51121 gaagaccggc gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg catctgcttc
    51181 agaagctgaa ccgttgatga tggtgttcat cagcgtgtac cagtcttttt cgatgcgatg
    51241 catgtacaga cggctttcac cgcgagctac cgggtaaaca ggcatcagtg gcggatgcgg
    51301 gaaacgctca tccagatatt ccataatgat gcgagattcc cacagggtca gctcacgatc
    51361 caccagggtc ggaacgctct gattcgggtt gaggtcaatc agatcctgag gcggattgtc
    51421 cttttccacg tgttcgatct cgaaacttac acctttctca gccagcacaa tgcggacctg
    51481 atggctatag atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt tgttggcagc
    51541 gacagccatg aaaacctcca ggtatagtca gaatttttac tgctaccagc caccaggtgg
    51601 ccagtcagaa gttgtgttac ccaataagga acgactctct ttgttcgaaa atcaaacaaa
    51661 aaatgagcaa tacccgacat ttgggcagaa aattggatga tagtttacca gattttgcga
    51721 ccattgtggt gagtggatgc cggaaatggg gaaaaagaga tgcgctttag tctgaaatag
    51781 ttgacttaga cccttattgg cgatgtggtt tttgttttgc ctgtctttca gacggcagca
    51841 aaaagcaact tcccattttt tacgctgatt cagaatttag ccataaaaaa acccgccgaa
    51901 gcgggttttt tcgtaaattg ttttctgccg gagcagaagc caattaacgt ttggagaact
    51961 gcggacgacg acgtgcttta cgcagaccga ctttcttacg ttcaacctga cgagcgtcac
    52021 gagtaacgaa gccagcttta cgcagttcag aacgcaggga ctcgtcgtat tccatcagag
    52081 cgcgggtgat accgtgacgg atcgcaccag cctgaccaga gataccacca cctttaacgg
    52141 tgatgtacag gtccagtttc tcaaccatgt cgaccagttc cagcggctga cgaactacca
    52201 tgcgggcagt ttcacgaccg aagtactgtt ccagagaacg ttggttgatt acgattttac
    52261 cgttgcccgg tttgatgaaa acgcgagctg cggaattttt gcggcgacca gtgccgtagt
    52321 attgattttc agccattgcc tataatcccg attagatgtc aagaacttgc ggttgctgtg
    52381 ccgcgtggtt gtgctcgtta cccgcgtaaa ctttcagttt acggaacata gcacgaccca
    52441 gcgggccttt tggcaacatg cctttaaccg cgatttcaat cacacgctca ggacggcgag
    52501 caatcatctc ttcaaagctc acttgtttga taccaccgat gtggccggtg tggtgatagt
    52561 acactttgtc agtacgcttg ttgccggtta cagcaacttt gtcagcgttc agaacgatga
    52621 tgtaatcacc ggtatctacg tgcggagtgt attccgcttt gtgcttaccg cgtaggcgac
    52681 gagccagttc agtagccaga cggcccagag ttttaccggt cgcgtcaaca acataccagt
    52741 cgcgttttac ggtttctggt ttagctgtaa aagttttcat taaaagctta cccaataaat
    52801 agttacacgt tggtgaacac ccaaacgtct tcaattgttg aggttcacac gacaaagtcc
    52861 ggcaaaccta ccccttcgaa tagcctatgc cagcacacaa aaagttttgg gaaaaaaact
    52921 ttcttgtaac gtggggtcgc aggattatag agaagtcggg gtcaaagatc gacccctttt
    52981 tgtgatttgt gacaggtttt aacccgccaa atgctcgcgc ttcagatact cttcgctttg
    53041 catctcttgc agacgtgaca ggcaacgctg gaactcaaac ttcagccgat cgccctgata
    53101 aatttcatac agcggcactt ctgcactcac cactaatttg acatggcgct cgtaaaactc
    53161 atccaccagc gcaataaagc gccgcgcttc gctctccatc aaccgcgtca taactggtac
    53221 atcaaacaac atgaccgtat gaaagagacg tgagagcgca atatagtcat gctgactgcg
    53281 ggcgtcgacg cacagcgtag taaaagagac cgccagcgtc tggttctcga cgcccattgt
    53341 cgctaatggc cgatggttga tttctaacgt cggtgaattt tctcgtttcc cccccgccag
    53401 cgccaaccat agtttatcca tttgcgcccg ggtttcatcg tggagtggcg aaagccacag
    53461 atgcgcctga gtgagtgtac gcagtcgata atcaacacca gcgtccacgt tcattacatc
    53521 acaatgctgt ttaatggcat cgattgcagg cagaaaacgc gcacgttgca agccatttcg
    53581 ataaagttcg tccggcggaa tatttgacgt cgctaccagg gtaataccgc gggcgaacaa
    53641 ggctttcatc agaccgccaa gtagcatggc atcggtaata tcagaaacaa aaaattcgtc
    53701 aaaacagagc acgtcagttt cggctttaaa gcgatcggca ataatttcca gcggatcggt
    53761 ctgcccctgt aaggcagtta gctcttcatg cacccgcagc ataaaacggt gaaagtgcag
    53821 gcgctgtttc cgctctcccg gcaggctttg atagaaaagg tccatcagcc aggttttccc
    53881 gcgtcctaca ccgccccaca tatataagcc acgcactggc gtatgctttg tgtcttcgcg
    53941 tttaccccac agcttaccga cccgcgccat tagcccactc gtcctggggg ctggtggcgt
    54001 gctattgatg agttcctgat aaataatttc caggcggctg acggcctctt tttgaacgtc
    54061 gtcgggttga tggctgcctt cattgagcgc cttcaggtat tgcgatgttg gggtaacgct
    54121 ttgcatgatc ttattgttat tccttgaata atcggtgcgc cgttgttcac ggttgacgaa
    54181 aaaaaggccg ttctacacta cgcgatatgc agtcgggatt ccacttctgt ggaattaacg
    54241 gttatagtgg cataatcagc cgcaggcatg gagcctgaag ccaacaccct acggaaacaa
    54301 aagacaacgg gagatgttca tgacctggga atatgcgcta attgggttag tcgtcggcat
    54361 cattattggt gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc aacaggcgtt
    54421 gcagtacgaa ctggaaaaga ataaagctga actggacgag tatcgcgaag agctggttag
    54481 ccactttgcc cgcagcgcgg aattactgga taccatggcg cacgactatc gccagctgta
    54541 tcagcacatg gcgaaaagtt ccagcagcct gctgccggaa ctgtctgctg aagcaaaccc
    54601 gttccgtaat cgtctggcag agtctgaagc cagcaacgat caggcaccgg tgcagatgcc
    54661 tcgcgactat tctgaaggcg catccggcct gctgcgtact ggcgcgaagc gcgactaatt
    54721 tatttttcgg gcgcagccat tgcgccctcc tcttctctcc ctccccgact atcatttaat
    54781 ctggtgtctc attgttagcc gtctgaaaat tcaataacat caaactgttt tgaatctctt
    54841 ttcttatcat tcaggtacga gagcaggaat aatgaaaaaa caaccccagc tgttgagtgc
    54901 attagcgtta agtgtcgggt taactctctc ggcgtcattt caggccgtcg cgtcgattcc
    54961 aggccaggtt gccgatcagg cccctctccc cagtctggct ccaatgctgg aaaaagtgct
    55021 tccggcagtg gtgagcgtac gggtggaagg aacggccagt cagggacaga aaatcccgga
    55081 agaattcaaa aagttttttg gtgatgattt accggagcaa cctgcacaac ccttcgaagg
    55141 tttaggctcc ggtgtcatca tcaacgccag taaaggctat gtgctgacca acaaccatgt
    55201 gattaatcag gcacagaaaa tcagtattca gctcaatgat gggcgcgagt ttgatgcaaa
    55261 actgattggt agcgatgacc agagcgatat cgccctgtta caaattcaaa acccgagcaa
    55321 attaacgcaa atcgctattg ccgactccga taaattgcgc gtcggtgatt ttgccgtagc
    55381 ggtcggtaac ccatttggcc ttgggcaaac cgccacctct ggcattgttt ccgcattagg
    55441 acgcagcggg ttgaatcttg aaggtctgga aaactttatc cagacagatg cttccattaa
    55501 ccgcggtaac tccggcggtg cactgttaaa ccttaacggt gagttaattg gcatcaacac
    55561 tgcaatcctt gcgcctggcg gcgggagcgt cgggattgga tttgccatcc ccagtaatat
    55621 ggcgcgaaca ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg gtttgttagg
    55681 catcaaaggc accgagatga gtgccgatat cgccaaagcc ttcaaccttg acgtgcagcg
    55741 tggcgcgttt gtcagcgaag tgttgccagg ttctggctcg gcgaaagcgg gcgtcaaagc
    55801 gggcgatatt attaccagcc tcaacggcaa accgctgaat agctttgctg agttgcgctc
    55861 tcgtatcgcg accaccgagc cgggcacaaa agtgaaactt ggcctgctgc gtaacggcaa
    55921 accactggaa gtagaagtga cgctcgatac cagcacctct tcgtcggcca gcgctgaaat
    55981 gatcgcgcca gcgctggaag gtgcaacgtt gagcgatggt cagctaaaag atggcggcaa
    56041 aggtattaag atcgatgaag ttgtcaaagg aagcccagct gctcaggctg gcttgcaaaa
    56101 agacgatgtg atcattggcg tcaaccgcga tcgggtgaac tcgattgctg aaatgcgtaa
    56161 agtgctggcg gcaaaatcgg ccatcatcgc cctgcaaatt gtacgcggca atgaaagcat
    56221 ctatctgctg atgcgttaat gtcgtaaacc gggcatcagg cttacgtgtg atgtccggtt
    56281 aactcgtggt atgctgctgc cgttcccttt tttaatgacg cctccatcat gtttgtgaag
    56341 ctcttacgtt ccgttgcgat tggattaatt gtcggcgcta ttctgctggt tgccatgcct
    56401 tcgctgcgca gccttaaccc gctttccact ccgcaatttg acagtaccga tgagacgcct
    56461 gccagctata atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt ttacaaccgt
    56521 ggtttgaaca ccaactctca caaccagctt gagatccgca ccctgggatc cggtgtaatc
    56581 atggatcaac gcggttatat catcaccaat aaacacgtca tcaacgacgc cgatcagatc
    56641 atcgtcgcct tacaggatgg tcgtgtattt gaagcattgc tggtgggatc tgactctcta
    56701 accgatctgg cggtacttaa aattaatgcc actggcggtt tacctaccat tccaatcaat
    56761 gcacgtcgcg taccgcatat tggcgacgtg gtactggcga tcggtaaccc gtacaacctc
    56821 gggcagacca ttacccaggg gattattagt gccacgggtc gaattggtct gaacccgacc
    56881 gggcggcaaa acttcctgca aaccgatgct tccattaacc acggtaactc tggcggcgcg
    56941 ctggtgaact cgctgggcga actgatgggc attaacacgc tgtcgtttga taagagtaac
    57001 gatggcgaaa cgccggaagg tatcggcttt gcgattcctt tccagttagc aaccaaaatt
    57061 atggataagc tgatccgcga tggtcgcgtg atccgcggct acattggcat tggcggacgt
    57121 gagatcgcac cgctgcacgc acagggcggt ggtatagatc aactgcaagg gatcgtggtt
    57181 aatgaagtgt cacctgacgg cccggcggcg aatgcgggta ttcaggtcaa cgatctgatt
    57241 atttcggtgg ataacaaacc ggccatctct gctctggaga cgatggatca ggtggcggaa
    57301 attcgccctg gttcggtgat cccggttgta gtgatgcgtg atgataagca gttaacgctg
    57361 caggtcacca ttcaggaata tccggcaacc aattaagtcg agtgctcaaa acaaaaaacc
    57421 ggagtctgtg
//
LOCUS       DAETQN010000012        52616 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.012, whole genome shotgun sequence.
ACCESSION   DAETQN010000012 DAETQN010000000
VERSION     DAETQN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52616
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.012"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            450..602
                     /gene="mokC"
                     /locus_tag="K0W68_000778"
     CDS             450..602
                     /gene="mokC"
                     /locus_tag="K0W68_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBI3072431.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(706..1836)
                     /gene="dnaJ"
                     /locus_tag="K0W68_000779"
     CDS             complement(706..1836)
                     /gene="dnaJ"
                     /locus_tag="K0W68_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBI3072432.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1925..3841)
                     /gene="dnaK"
                     /locus_tag="K0W68_000780"
     CDS             complement(1925..3841)
                     /gene="dnaK"
                     /locus_tag="K0W68_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBI3072433.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4218..4622
                     /locus_tag="K0W68_000781"
     CDS             4218..4622
                     /locus_tag="K0W68_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBI3072434.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4648..5361
                     /gene="msyB"
                     /locus_tag="K0W68_000782"
     CDS             4648..5361
                     /gene="msyB"
                     /locus_tag="K0W68_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBI3072435.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5510..6076
                     /gene="satP"
                     /locus_tag="K0W68_000783"
     CDS             5510..6076
                     /gene="satP"
                     /locus_tag="K0W68_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBI3072436.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6111..6698)
                     /gene="mog"
                     /locus_tag="K0W68_000784"
     CDS             complement(6111..6698)
                     /gene="mog"
                     /locus_tag="K0W68_000784"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBI3072437.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6813..7766)
                     /gene="tal"
                     /locus_tag="K0W68_000785"
     CDS             complement(6813..7766)
                     /gene="tal"
                     /locus_tag="K0W68_000785"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBI3072438.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8045..9474
                     /locus_tag="K0W68_000786"
                     /pseudo
     CDS             8045..9474
                     /locus_tag="K0W68_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            9544..10320
                     /gene="yaaA"
                     /locus_tag="K0W68_000787"
     CDS             9544..10320
                     /gene="yaaA"
                     /locus_tag="K0W68_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBI3072439.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10473..10769)
                     /locus_tag="K0W68_000788"
     CDS             complement(10473..10769)
                     /locus_tag="K0W68_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBI3072440.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10983..12269)
                     /gene="thrC"
                     /locus_tag="K0W68_000789"
     CDS             complement(10983..12269)
                     /gene="thrC"
                     /locus_tag="K0W68_000789"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBI3072441.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12270..13202)
                     /gene="thrB"
                     /locus_tag="K0W68_000790"
     CDS             complement(12270..13202)
                     /gene="thrB"
                     /locus_tag="K0W68_000790"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBI3072442.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13204..15666)
                     /gene="thrA"
                     /locus_tag="K0W68_000791"
     CDS             complement(13204..15666)
                     /gene="thrA"
                     /locus_tag="K0W68_000791"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBI3072443.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15693..15812)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15748..15813)
                     /gene="thrL"
                     /locus_tag="K0W68_000792"
     CDS             complement(15748..15813)
                     /gene="thrL"
                     /locus_tag="K0W68_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBI3072444.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16027..16713)
                     /locus_tag="K0W68_000793"
     CDS             complement(16027..16713)
                     /locus_tag="K0W68_000793"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBI3072445.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17113..17253)
                     /locus_tag="K0W68_000794"
     CDS             complement(17113..17253)
                     /locus_tag="K0W68_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072446.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17349..18065
                     /gene="arcA"
                     /locus_tag="K0W68_000795"
     CDS             17349..18065
                     /gene="arcA"
                     /locus_tag="K0W68_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBI3072447.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18125..19477)
                     /gene="creD"
                     /locus_tag="K0W68_000796"
     CDS             complement(18125..19477)
                     /gene="creD"
                     /locus_tag="K0W68_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBI3072448.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19535..20959)
                     /gene="creC"
                     /locus_tag="K0W68_000797"
     CDS             complement(19535..20959)
                     /gene="creC"
                     /locus_tag="K0W68_000797"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBI3072449.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20959..21648)
                     /gene="creB"
                     /locus_tag="K0W68_000798"
     CDS             complement(20959..21648)
                     /gene="creB"
                     /locus_tag="K0W68_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBI3072450.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21661..22134)
                     /gene="creA"
                     /locus_tag="K0W68_000799"
     CDS             complement(21661..22134)
                     /gene="creA"
                     /locus_tag="K0W68_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBI3072451.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22345..23214
                     /gene="robA"
                     /locus_tag="K0W68_000800"
     CDS             22345..23214
                     /gene="robA"
                     /locus_tag="K0W68_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBI3072452.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23211..23858)
                     /gene="gpmB"
                     /locus_tag="K0W68_000801"
     CDS             complement(23211..23858)
                     /gene="gpmB"
                     /locus_tag="K0W68_000801"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBI3072453.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23910..24431
                     /gene="yjjX"
                     /locus_tag="K0W68_000802"
     CDS             23910..24431
                     /gene="yjjX"
                     /locus_tag="K0W68_000802"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HBI3072454.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24516..24842)
                     /gene="trpR"
                     /locus_tag="K0W68_000803"
     CDS             complement(24516..24842)
                     /gene="trpR"
                     /locus_tag="K0W68_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBI3072455.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24932..26869)
                     /gene="sltY"
                     /locus_tag="K0W68_000804"
     CDS             complement(24932..26869)
                     /gene="sltY"
                     /locus_tag="K0W68_000804"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBI3072456.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27080..28747
                     /gene="ettA"
                     /locus_tag="K0W68_000805"
     CDS             27080..28747
                     /gene="ettA"
                     /locus_tag="K0W68_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBI3072457.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(29054..30286)
                     /gene="nadR"
                     /locus_tag="K0W68_000806"
     CDS             complement(29054..30286)
                     /gene="nadR"
                     /locus_tag="K0W68_000806"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HBI3072458.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30307..31689)
                     /gene="radA"
                     /locus_tag="K0W68_000807"
     CDS             complement(30307..31689)
                     /gene="radA"
                     /locus_tag="K0W68_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBI3072459.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31738..32706)
                     /gene="serB"
                     /locus_tag="K0W68_000808"
     CDS             complement(31738..32706)
                     /gene="serB"
                     /locus_tag="K0W68_000808"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBI3072460.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32812..33456
                     /locus_tag="K0W68_000809"
     CDS             32812..33456
                     /locus_tag="K0W68_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBI3072461.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33484..34500
                     /gene="lplA"
                     /locus_tag="K0W68_000810"
     CDS             33484..34500
                     /gene="lplA"
                     /locus_tag="K0W68_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HBI3072462.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34532..34681
                     /locus_tag="K0W68_000811"
     CDS             34532..34681
                     /locus_tag="K0W68_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072463.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(34956..35675)
                     /gene="deoD"
                     /locus_tag="K0W68_000812"
     CDS             complement(34956..35675)
                     /gene="deoD"
                     /locus_tag="K0W68_000812"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBI3072464.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35755..36978)
                     /gene="deoB"
                     /locus_tag="K0W68_000813"
     CDS             complement(35755..36978)
                     /gene="deoB"
                     /locus_tag="K0W68_000813"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBI3072465.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(37030..38352)
                     /gene="deoA"
                     /locus_tag="K0W68_000814"
     CDS             complement(37030..38352)
                     /gene="deoA"
                     /locus_tag="K0W68_000814"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBI3072466.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38479..39258)
                     /gene="deoC"
                     /locus_tag="K0W68_000815"
     CDS             complement(38479..39258)
                     /gene="deoC"
                     /locus_tag="K0W68_000815"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBI3072467.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39516..41066
                     /locus_tag="K0W68_000816"
     CDS             39516..41066
                     /locus_tag="K0W68_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBI3072468.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            41038..41901
                     /locus_tag="K0W68_000817"
     CDS             41038..41901
                     /locus_tag="K0W68_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBI3072469.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(42417..43199)
                     /locus_tag="K0W68_000818"
     CDS             complement(42417..43199)
                     /locus_tag="K0W68_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBI3072470.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(43196..44269)
                     /locus_tag="K0W68_000819"
     CDS             complement(43196..44269)
                     /locus_tag="K0W68_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBI3072471.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(44391..44552)
                     /locus_tag="K0W68_000820"
     CDS             complement(44391..44552)
                     /locus_tag="K0W68_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBI3072472.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(44679..45284)
                     /gene="osmY"
                     /locus_tag="K0W68_000821"
     CDS             complement(44679..45284)
                     /gene="osmY"
                     /locus_tag="K0W68_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBI3072473.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(45677..47266)
                     /gene="prfC"
                     /locus_tag="K0W68_000822"
     CDS             complement(45677..47266)
                     /gene="prfC"
                     /locus_tag="K0W68_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBI3072474.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(47357..48034)
                     /gene="yjjG"
                     /locus_tag="K0W68_000823"
     CDS             complement(47357..48034)
                     /gene="yjjG"
                     /locus_tag="K0W68_000823"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBI3072475.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(48049..48495)
                     /gene="rimI"
                     /locus_tag="K0W68_000824"
     CDS             complement(48049..48495)
                     /gene="rimI"
                     /locus_tag="K0W68_000824"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBI3072476.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(48464..48877)
                     /gene="holD"
                     /locus_tag="K0W68_000825"
     CDS             complement(48464..48877)
                     /gene="holD"
                     /locus_tag="K0W68_000825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBI3072477.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            48980..50011
                     /gene="rsmC"
                     /locus_tag="K0W68_000826"
     CDS             48980..50011
                     /gene="rsmC"
                     /locus_tag="K0W68_000826"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBI3072478.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            50279..50365
                     /locus_tag="K0W68_000827"
     tRNA            50279..50365
                     /locus_tag="K0W68_000827"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50313..50315,aa:Leu,seq:cag)
     gene            50394..50480
                     /locus_tag="K0W68_000828"
     tRNA            50394..50480
                     /locus_tag="K0W68_000828"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50428..50430,aa:Leu,seq:cag)
     gene            50515..50601
                     /locus_tag="K0W68_000829"
     tRNA            50515..50601
                     /locus_tag="K0W68_000829"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50549..50551,aa:Leu,seq:cag)
     gene            complement(50640..50876)
                     /locus_tag="K0W68_000830"
     CDS             complement(50640..50876)
                     /locus_tag="K0W68_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBI3072479.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            51017..51805
                     /gene="fhuF"
                     /locus_tag="K0W68_000831"
     CDS             51017..51805
                     /gene="fhuF"
                     /locus_tag="K0W68_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBI3072480.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(51843..52520)
                     /gene="bglJ"
                     /locus_tag="K0W68_000832"
     CDS             complement(51843..52520)
                     /gene="bglJ"
                     /locus_tag="K0W68_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBI3072481.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(52478..>52616)
                     /locus_tag="K0W68_000833"
     CDS             complement(52478..>52616)
                     /locus_tag="K0W68_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBI3072482.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
BASE COUNT        12437 a        14232 c        13233 g        12714 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa taggcgaggc
      241 gtcttcagga ttagaatcga taaaacagaa tgggttaaag agagcgatat aatagcggcg
      301 ggtgcttgag gctatctgtc tcaggcatta gctgaacggc agatagagaa aagccccgag
      361 tgatatttta ctatcaaccc gaggccccct atatgctgaa cacatgtaga gtgcctctta
      421 ctgaccgtaa ggtcaaggag aagagagcaa tgaagcagca taaggcgatg attgtcgccc
      481 tgatcgtcat ctgtatcacc gccgtagtgg cggcgctggt aacgagaaaa gacctctgtg
      541 aggttcacat ccgaactggc cagacggagg ttgctgtttt cacggcttac gaatccgagt
      601 aagagcaacg gcggggagtg atccccgcca cttttcaggt gctcgcatat cttcaacgca
      661 ccctaatttt acccaagcct gcccacgggc aggcttttgg ggaggttagc gggtcaggtc
      721 gtcaaaaaac ttcttcacac catcaaagaa gctctttgag cgcgggctgt tgtgctcacc
      781 ggttgggcca ccgaagcttt cttgcagctc ttgcagcaac tgtttctgct tctcgttcag
      841 gcctaccggt gtttcgacga caacgcggca cagcaaatca ccctgtgcgc caccgcggac
      901 agacttgacg cctttaccgc gcatacggaa aagcttaccg gtctgggttt cgccaggcac
      961 tttcagtttg acgcgaccat caagggtcgg tacttcgatt tcgccaccca gcgccgccat
     1021 agcgaagttg atcgggactt cgcaatagag gttgttgcct tcacgctcga aaatcgggtg
     1081 ctgtttaacc tgaacctgaa cgtacagatc gcctgccggt gcgccatgct cgcccgcttc
     1141 accttcgccc gcaagacgga tgcggtctcc agtgtccacc cctgccggga ttttaacgga
     1201 cagcgttttg ctgcgctcaa cacgaccatg accatgacat ttgttgcacg gatctttgat
     1261 cagcgtaccg cggccctgac agtgtggaca ggtctgctgt acagcgaaaa atccctggcg
     1321 catctgcacc tgaccagaac catgacaggt cggacaggtc tgcggctgtg tacctggttt
     1381 tgcaccgcta ccgtggcaaa cgtcacactc ttccagagtc ggaatgcgga tctctttggt
     1441 cacgccacgt acagcttctt cgagggtgag ctccatgtta tagcgtaaat cagcaccgcg
     1501 cgccgcacgt tgacgaccac gtccgccgcc aaaaatatcg ccgaaaacgt caccaaaaat
     1561 atcgctgaag tctgcgccgc cgccaaaacc gccgccgccc atgccacctt gctcaaacgc
     1621 agcatgacca tactgatcgt atgccgcacg tttttgcgag tcggtcagaa cttcataagc
     1681 ttccttgatc tctttaaatt tcgcctcggc ctctttgtca ccctggttac ggtccgggtg
     1741 gtatttcatg gccaggcgtt tgtaggcctt tttgatttca cgctcttccg ctgttttgga
     1801 aacgcctaaa atctcgtaat aatcttgctt agccatcttt tttaaattgc ccctagatga
     1861 atgcacgggc ggagaggaaa ttccccttcg cccgtgtcag tataattacc cgtttatagg
     1921 gcgattattt tttgtctttg acttcttcaa attcagcgtc gacaacatcg tcatctttcg
     1981 cgttgtttgc agaagcatca gcaccggcag tctgctgctg ggcatgttgc tgctgggcga
     2041 tttccatcag tttctgggaa acctgtgcca gttcctgcat tttcgcttcg atagcggctt
     2101 tgtcttcacc tttcagagca gtttccagtg cagtcagcgc agactcgata gcagttttgt
     2161 cgtcagccgg cagtttgtcg cctgcttctt caacctgctt acgggtgctg tgcagcagat
     2221 ggtcgccctg gttgcgagtc tgtaccagct cttcaaactt acggtcagct tcggcgttag
     2281 cttctgcgtc gcgtaccatt ttctggattt catcttcgtt cagaccagaa gaagccttga
     2341 tggtgatctt ctgctcttta ccgctgtttt tatctttcgc ggaaacgtgc aagataccgt
     2401 cagcatcgat atcgaaggta acttcgatct gcggcatgcc gcgcggtgcc gggttgatac
     2461 catccaggtt gaactgaccc agagatttgt tatcagctgc acgtttacgt tcaccctgca
     2521 gcacatggat ggttaccgca gactggttgt cttcagcggt agagaacacc tggctgtgct
     2581 tggtcgggat agtggtattt ttcgcgatca gcgtcgtcat cacaccgccc atggtttcga
     2641 tacccagaga cagcggggta acgtccagca gcagtacgtc ttttacgtca ccagtcagaa
     2701 caccaccctg aacagcagca ccgattgcta cagcttcgtc cgggttaacg tctttacgcg
     2761 gctctttacc aaagaactca gcaactttct tctgaaccat tggcatacga gtctgaccac
     2821 ccacgaggat aacgtcgtcg atatcagata cggacaggcc agcgtcctgc agtgcaactt
     2881 tcagcggctc aatggaacgg tttaccagat cttcaaccag gctttccagt ttcgcacgag
     2941 tcactttgat gttcatgtgt ttcggaccgg tcgcatctgc agtgatgtac ggcaggttaa
     3001 cgtcggtctg ctgagcggaa gacagttcga ttttcgcttt ttctgccgct tctttcaggc
     3061 gctgcattgc cagcggatcg ttgcgcaggt caatgccctg atctttcttg aattcttcaa
     3121 ccaggtagtt gatcagacgg ctgtcgaagt cttcaccccc caggtgggta tcaccgttgg
     3181 tagccagaac ttcgaaggtt ttttcgccgt caacttcgtc gatttcgata atagaaatat
     3241 cgaaagtacc accacccagg tcataaaccg cgatagtacg gttgccagta cctttgtcca
     3301 gaccgtaagc cagcgcagct gcggtcggtt cgttgatgat acgttttact tccagaccag
     3361 cgatacggcc tgcgtctttg gttgcctgac gctgagcatc gttaaagtat gccggtacgg
     3421 tgataacagc ttcagttacc ggttcaccca agtaatcttc agcggttttc ttcatttttt
     3481 tcagcacttc agcagaaatc tgcggcggtg ccattttctg gcctttaact tcgacccatg
     3541 cgtcgccgtt atcagcagca ataattttga acggcatgat ggaaacatca cgctgtactt
     3601 cttcgtcctg gaagcggcgg ccaatcaggc gtttaatcgc aaacagggtg ttttgcgggt
     3661 tcgtcactgc ctgacgttta gccggctgac caaccagagt ttcaccatcc tgggtatagg
     3721 caataataga aggcgtggtg cgatcgcctt cggcgttctc cagcacgcga ggagtggtgc
     3781 catccataat cgctacacaa gagttggtag tacccaggtc gataccaatt attttaccca
     3841 tctaaacgtc tccactatat attcggtcat catgtggttg tgagtctgta ataggggcga
     3901 aacgtctggt ttcaactgcc caatttcatt tttttgcaga ctcactcact gcggttgact
     3961 actaaatggg gtcgtaaccc acgtcatcaa gggggagatg caaaaatttt ttgtgctttt
     4021 gcgcagaaat ttcgcgatat ggcaggacaa gacgtaaaat tgacagcaat tttattcata
     4081 taaagaatga atcgcagcca aggcattcat caaaaaattg taataaaaag aaaagattac
     4141 gtacctgaat cttctcttta tcagcagtaa actagtgggt attcatccct ctacctcttc
     4201 ccactaagag aatccttatg aaatccgttt ttacgatttc cgccagcctg gcgattagcc
     4261 tgatgctgtg ctgcacggcg caggcaaacg accataaact cctcggcgtc attgcaatgc
     4321 cgcgtaacga aaccaacgat ctggcgctga aacttcctgt ttgtcgcatt gtgaaacgca
     4381 tacaactctc tgccgaccat ggcgatttac agttaagcgg tgcatcggtt tatttcaaag
     4441 ccgcccgtag cgccagtcag agtctgaata ttccttcaga aataaaagaa gggcaaacca
     4501 ctgactggat caacattaac agtgataacg acaataaacg ctgcgtctca aaaatcacct
     4561 tttccggtca tacggtgaac tcatcggata tggccacgct gaaaattatc ggcgacgact
     4621 aacgccagaa tatagaaacc acaaaaaatg aatgttaatt acctgaatga ttcagatctg
     4681 gattttcttc agcattgtag tgaggaacag ttggcaaatt tcgcccgatt gctcacccat
     4741 aatgaaaaag gcaaaactcg cctctccagc gtactgatgc gcaacgaact gtttaaatcg
     4801 atggaagggc atcccgagca acatcgccgc aactggcagc tgattgctgg agaattacag
     4861 cattttggtg gcgatagtat cgccaacaaa ctgcgcggac acggtaaatt gtatcgggcc
     4921 attttgctcg atgtttcaaa acgattgaag ctgaaagccg acaaagagat gtctacgttt
     4981 gagattgagc aacagttact ggaacaattt ctgcgtaaca cctggaagaa tatggacgag
     5041 gaacataagc aggagtttct gcacgcggtc gatgccaggg tgaatgagct ggaagagctg
     5101 ctgccgctgc tgatgaaaga caaattattg gcaaaaggcg tgtcgcattt gctttccagc
     5161 caattgaccc gcattttacg cacccacgca gcaatgagcg tgcttgggca tggtttgctg
     5221 cgcggcgcgg gtctgggagg ccctgtgggc gcagcattaa atggggttaa agcggtcagc
     5281 ggcagtgcct atcgcgtgac aattccagcc gtaatacaaa tcgcctgcct gcgccggatg
     5341 gttagcgcca ctcaggtctg acaccgtaaa ccggggaaaa ttttcatttt cccctcaaaa
     5401 gatcgtagac actgccccac tggctgatta ttatgccgcg ccctgaaaac actactgtta
     5461 ttcagggaaa ttatttcacc attcattcga tgatgatttt tgaggaatta tgggcaacac
     5521 taagttggct aatccggcac cgctgggcct gatgggcttc ggcatgacca ccattctgct
     5581 taacctgcac aacgtgggtt atttcgctct ggacggtatt attcttgcca tgggcatttt
     5641 ctacggcggc atcgcgcaaa tttttgccgg tctgctggag tacaaaaaag gcaacacttt
     5701 cggtttaacc gcattcacct cttacggttc tttctggttg acgctggttg cgattctgct
     5761 gatgccgaaa ctgggtctga ccgatgcgcc aaatgcacag ttccttggtg tctacctggg
     5821 tctgtggggc gtatttacgc tgtttatgtt cttcggcacg ctgaaaggcg cacgcgttct
     5881 gcaattcgtt ttctttagcc tgactgtgct gtttgccctg ctggcgatcg gtaacattgc
     5941 cggtaacgcc gcaatcatcc actttgccgg ctggattggt ctgatctgtg gtgccagtgc
     6001 aatctatctg gcgatgggtg aagtactgaa cgagcagttt ggtcgcaccg ttctgccgat
     6061 tggtgaatcc cactaattgt tttgagatcc ccccgctcgg ggggattttt ttattcgcta
     6121 acgtcgcgtc ttgcactctt cggtctgaat gcagcaacca cttctggtgc cgtttcaacg
     6181 tatggccctt ccagcaactg aatgcagtac ggtacgctgg caaaaatacc gtgtaccaca
     6241 acgttacccg cagcgtcctt cacaccttcc agcgtctctt taatagactt cggctgaccg
     6301 ggtaagttaa ggatcagcgc ctgtttgcga atcacaccaa cctgacgcga aaggatcgca
     6361 gttggtacaa aatgcaggct tatctggcgc atctgttcac caaagccagg catctcgcgg
     6421 tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac gcgccgggcc agttccgccc
     6481 gtggtgagca ccagatggca actcatttca tccactagct cacacaacgt ttgctcgatg
     6541 atcgcctgct catcggggat taagcgggtt tccagttcaa acggcgtggt tagcgccgat
     6601 gtcagccatt cttccagcgc agggatgcct ttatcctgat aaacgccgct ggatgcgcga
     6661 tcagagatgg aaactaagcc aatacgtaaa gtattcatca agatttttcc gtttaaacag
     6721 tctcgttaaa cagaatgata cactgcgaag ggagtgacag acaggcttca gaagaggtag
     6781 cgtgaccgac ttcccggtca cgctaagaat gattacagca gatcgccgat cattttttcc
     6841 agtttttcct ggtcaatagc aaacttacgg ataccttccg ccagtttatc tactgccatt
     6901 ggatcctggt tgtgctgcca caggaactcg gactcagtga tacgcgccgg acgcgctttc
     6961 acttcgccgg tgtaagacag tttacgttcg atagcccctt cgctctcagc cagctctttc
     7021 agcagtgccg gtgcgatggt aagacggtcg cagcctgcca gttccagaat ttcgccgatg
     7081 ttacggaagc ttgcgcccat aaccacggtt tcataaccgt gctctttgta gtactggtag
     7141 atttcagata cagaaaccac gcccggatct tctgccggag cgtactcttt cttatcagta
     7201 ttcgctttgt accagtcaag aatacggcca acaaatggcg agatcaggaa cacgcctgct
     7261 tccgcacaag cacgagcctg agcgaaggag aacagcaggg tcaggttaca gttgatgcct
     7321 tctttttcca gctgttctgc agcacggata ccctgccaag tagaagccag tttgatcaga
     7381 atacgatcgt tgctaatacc agcatcgttg tagagtttga tcaggcgttt tgctttcgca
     7441 attgacgctt cggtgtcata ggaaagacgc gcatcaactt cagttgagat acggcccgga
     7501 accagtttca ggatttccag accaatattt actgccagtt tgtcggtcgc gtccacgatc
     7561 tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga cagcatcatc aatcaactta
     7621 cggtattccg gaatctgcgc tgcgttaaga atgagagaag ggttggttgt ggcatcctgc
     7681 ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg ccactacggt ggtgtactga
     7741 cgaagggagg tcaatttgtc cgtcatgata gtatttctct ttaaacagct tgttaggggg
     7801 atgtaaccgg tctgccctga tgatatcacg acgcaacgtc agcgcaaccg ccgacaagac
     7861 ggttatcgcc aggcgagact gtttcggatt tctgacactg cgttgatatc gcccgccatt
     7921 tttatacaaa acctcatgta tgctacgcag aagttatcaa gtacctcgta gcgtatatac
     7981 ttcttaaaca attggtaacg tttacacagg aaagtcatcg cgaccggcaa taagagggat
     8041 atgcatgcca gattttttct cctttattaa cagcgtcctt tggggatcgg taatgattta
     8101 cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc ggatttgtgc agtttcgcta
     8161 catccgccag tttggcaaaa gtcttaaaaa tagcattcat ccacagccag gcggtttaac
     8221 ctcatttcag tcattgtgta ccagtcttgc ggcgcgcgtg ggtagcggca acctggccgg
     8281 cgttgcgctg gctattaccg ccggtggacc tggagccgtc ttttggatgt gggttgccgc
     8341 gtttatcggc atggcgacct cgtttgccga atgttccctt gcacaacttt ataaagaacg
     8401 tgacgtcaat ggacagtttc gtggaggacc ggcatggtat atggcgcgcg ggctggggat
     8461 gcgttggatg ggcgttctgt tcgccgtctt tttgctcatc gcctacggca taattttcag
     8521 cggagttcag gcgaacgccg ttgcccgagc cctgagtttt cttttgattt tcccccgctg
     8581 gtgacaggca ttattctcgc tgtctttgct ctgctggcaa tcactcgcgg tcttcatggc
     8641 gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga taatctgggt gctgaccagc
     8701 atggtgattt gcgtaataaa tatcgggcaa cttccccacg tcatttggtc tatttttgag
     8761 agtgcttttg gctggcagga agcggcaggc ggcgcggcgg gatatacctt aagccaggcg
     8821 attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg ttcgacgcca
     8881 aacgcggcag cggcagcggc gtcctggcct ccgcatccgg cagcgcaagg gattgtccag
     8941 atgattggca tttttatcga caccctggtc atctgtacgg caagcgccat gctgatatta
     9001 ctggcgggta acgacacaac ctacatgccg ctggaaggta ttcagcttat ccagaaggcg
     9061 atgcgggtgc taatgggttc ctggggtgct gagtttgtta ccctcgtggt tattctgttt
     9121 gccttcagct ccatcgttgc caactacatt tatgccgaaa acaatctctt ctttttacgc
     9181 ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac ggtcatcggc
     9241 ggcaccttgc taagtcttcc gctgatgtgg caactggcag atatcataat ggcctgcatg
     9301 gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac cattgccagt
     9361 gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt gcgttatccg
     9421 gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga gtaatcacaa
     9481 ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct gcaaggactg
     9541 gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc gttgaccacc
     9601 acgcgctata cgctgccgga gctgttagac aattcccagc acttgatcca tgaagcgcgg
     9661 aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact ggcgggtatc
     9721 aacgccgctc gctttcatga ctggcagcca gatttcacgc cggagaatgc ccgccaggct
     9781 attttggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacttt cagcgaagac
     9841 gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg cgtactgcgc
     9901 ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga gaatgcccga
     9961 gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa cgaggcgctc
    10021 gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt taaatcggtg
    10081 aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga gaagaacggc
    10141 aaatttaaga taatcagctt ctacgctaag aaagcacgcg gtctgatgag tcgtttcatt
    10201 attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga aggttacttc
    10261 tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga gcagcgttaa
    10321 gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcatgcaga
    10381 ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg catcaggcgc
    10441 tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc tggaccatga
    10501 ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg cggtccgtgt
    10561 gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg gtcgcgccag
    10621 tgacctccat cccagtaata gccacgatta tcacgatcgc ctatctgtaa ttttactgac
    10681 gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac cagggaaagt
    10741 gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg cccgttatgg
    10801 gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca atcctaatct
    10861 ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttttc tccataattt
    10921 cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat aatgaataga
    10981 ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg cagattatgt
    11041 gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa cgtttcaccg
    11101 agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag gaacaagcca
    11161 tattcgcctg gattcaactg gtcacacagc gcacgatacg ctacggcagc gtgcggctcc
    11221 gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc atcatccacg
    11281 gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc ttccacacgc
    11341 ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg agtcgctttg
    11401 ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt ggtcgcagca
    11461 ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt caaatcgccg
    11521 aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg cagctgcgca
    11581 actgcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat cgagttagcc
    11641 gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt caccagcgcc
    11701 tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc caacgtacag
    11761 aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac cactttcaca
    11821 ttcggcaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga ggtcgcggtc
    11881 agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat aaagcgaccg
    11941 ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc gacatcgctt
    12001 tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc ttccaggatt
    12061 tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg ggtgacaaaa
    12121 tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc atgcggaaaa
    12181 aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct gacctgctcg
    12241 ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc gcccgccgta
    12301 tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt acccaaccag
    12361 tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt cgggccggag
    12421 ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc ctgccggaag
    12481 ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag cttcgcggca
    12541 agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg cccgtgcgca
    12601 atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc cgtcgagact
    12661 ttaattcccg gatacgccag cacccacagc cactcatcaa accctggcac ttgctggctg
    12721 atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg tgccacgttg
    12781 tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc ccatcaaagc cagcaaacgg
    12841 gtgtcattaa gtggcttgcc gcagtgttca ttcatcgcca tcagcgccgc gaccaccgaa
    12901 caggcgctgg agcctaagcc cgagccgatc ggcatattct tttccagagt catcgccact
    12961 ggaatttgct tgcccagctc caggcaaaaa cgctcccagc actgataaac gatattttcc
    13021 cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact gaatgtctct
    13081 gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacgggtgt caccgccgcc
    13141 ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata aactttaacc
    13201 atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga caccagcagc
    13261 ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct gataatagtg
    13321 gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat cattcccatc
    13381 cacttcggca atcttcacgc ggcagacacc atcttcatca atattgccaa catagcgcaa
    13441 aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt cgagctgtga
    13501 cagattcgcc ataaaagcgg caacatcacc ctcggcgtta aactctgcgg gcagcacagg
    13561 ttcaatttca atatccgcca gttccagttc acgtcccgtt tcacgagcga gaatcaatag
    13621 tttacgcgcc acatccatac cagaaagatc atctcgcggg tccggttcgg tataacccat
    13681 ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct tcgtctaact tgccgaagat
    13741 ataagaaagc gaacctgaaa gaatgccgga gaacttcacc aattcatcac ctgcattgag
    13801 cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt catagaggaa
    13861 tttacgccgc gatttttccg ccgcataacg caactgatga tagtaatcca tcgacgaggt
    13921 gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt cggcatattg
    13981 atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca gatgatattc
    14041 tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgtg caagttcttc
    14101 ctgccagttt tccagattaa ggccatgcac actagtgagc agagccttcg agttggcaac
    14161 gccgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgctgac gcttcagttg
    14221 ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga taacctgatc
    14281 ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat cgttatttac
    14341 cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt tgatattggc
    14401 gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca taccatcacc
    14461 taccaccgag ataatggcca gccgttccat caccgccagc ggctccagta agccttcttt
    14521 cagttccagg tagaactctt cctgcattgc ccgttcagcc cgcacacagt cgctttgcgg
    14581 aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca cggaaatacg
    14641 ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt tcatccccgg
    14701 gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga ccggtaattc
    14761 gccttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc cggtattttt
    14821 aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt gaagaacttt
    14881 agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca acaacctcgc
    14941 atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc aaatctcgca
    15001 acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac cgttgcgtcc
    15061 aagtaccacc agttcgcctt tttcattacc ggcggtgaag cctgccatca gcaccatgtg
    15121 atcagccgga atacgacttg ccgcaatacg gcgggtggac tcagcaatat cgacggtaga
    15181 ttcgaggtaa tgccccactg ccagtagttt ttcgaccgga tcgataacgg taacgttgtg
    15241 accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc cacggcaaat
    15301 cagcgcagcg ttaatgctat ccgggcactg ccctaacaaa ctaatgccat gcaggacatg
    15361 ttttatttgg gcaaattcct ggtcgacgaa agttttcaat tgcgccagcg ggaatcccgg
    15421 ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat cgctgatatt
    15481 gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt ggttggtgat
    15541 tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc tttccagaat
    15601 atcggcaacc cgcagaaaac gttctgcatt tgccactgat gtaccgccga acttcaacac
    15661 tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa agcccgcact gtcaggtgcg
    15721 ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg taatggtgat
    15781 ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg taatttttat
    15841 ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc aataaaattt
    15901 taatttactc atggcaggta accagttcag aagctgctat cagacactct ttttttaatc
    15961 cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata cttactaagg
    16021 cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca acattgccgt
    16081 gtctcgttgc tctaaaagcc ccaggcgttg ctgtaaccag tcgaccagtt ttatgtcatc
    16141 tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt gcagttgatg
    16201 ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta attgatagca
    16261 atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca tcggcacacc
    16321 agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt cgcgaccaaa
    16381 caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca ccagttctac
    16441 tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga cagtgaaatc
    16501 gacatcgtgt aacgattcag ccagtgttgg gaaaacttta atattatcaa taacatcacc
    16561 agatccatgt gcgacccagc gggtggctgg ctccaggtgt gcctgactat cgacaatccg
    16621 cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat tttctgctct
    16681 ggcgggtgcg accaggataa tcgttatacg catattgcca ctcttcttga ccaaataacc
    16741 gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat tcttaacgta
    16801 agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc tgactgtact
    16861 aacggtttaa ttgttaaaaa atgctacata tccttctgtt tacttaggat aattttataa
    16921 aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc aactagttgg
    16981 attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat caaactgtta
    17041 acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg gtgtccggcc
    17101 cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt ttgacactgt
    17161 cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt tgatggaaag
    17221 tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt aatttgcagc
    17281 atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg gcaatttagg
    17341 tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta acacgcaaca
    17401 cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca gatggcgcgg
    17461 aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat atcaatctgc
    17521 cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat gttgcgttga
    17581 tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa atcggtgcag
    17641 atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca cgcaacctgc
    17701 tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt gaaagctaca
    17761 agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct gatggcgagc
    17821 agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa aacccaggca
    17881 aaattcagtc ccgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg aaaccacacg
    17941 accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct acgccggata
    18001 cgccggaaat catcgccacc atccacggtg aaggttatcg cttctgtggt gatctggaag
    18061 attaatcggc tttaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt tttatttttc
    18121 aaccttattt ccagatacgt aattcatcgt ccgttgtaac ttctttactg gctttcattt
    18181 tcggcagtga aaacgcatac cagtcgatat tacgagtcac aaacatcatg ccggccagcg
    18241 ccaccaccag cacactggtt cccaacaaca gcgcgctatc gacagagttg agcagtcccc
    18301 acatcactcc atccagcaac aacagcgcga gggtaaacaa catgctattg cgccagcctt
    18361 tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg gcgattatcc
    18421 atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa aacatcacca
    18481 atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg agcgtttcaa
    18541 aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta gtcgcccggt
    18601 ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg aggaagtttt
    18661 cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca aaccagctgc
    18721 tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggc aaaaaatcac
    18781 ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta cgcccggcag
    18841 gcaccacaga aagatcgccg gtaccgctta aattcagggc catattcagc ttcaggttct
    18901 gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct tgctctaacc
    18961 cggtgccggg ttcaatggtc agcgccgttc cgttaacttc aggcgctttc accacaccaa
    19021 taccacgcgc atccccgacg ctaatcacaa taaatggctt gccaaaggtg atatttggcg
    19081 cgtcgagttc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa tcactgtgcc
    19141 agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg ccatcaacca
    19201 tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg gttttatcct
    19261 cttcctgtac cgtataaagc tcggtcacag gaatagcgat aagcggccca acaagttttt
    19321 gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa tcggcacgtt
    19381 cgacaatcac ctgccgaatc agcattatcg gaatcaacaa cagcaatact gcaccaaaca
    19441 gggtggtcat tttccagaac agggaggatt tcaacatagt cttctccttt gcaatggcag
    19501 caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg tgaagtcgaa
    19561 gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg ttaaacaaac
    19621 gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca tttgcacgcg
    19681 gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata ccactaccgg
    19741 tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc gttatacgac
    19801 cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc tgatccagta
    19861 acgccggttc agcagcaaca ttaacctcgg tgggcgtaac atgtagagtg atgttttttt
    19921 ctgccaactg cacggtgcgc gcttcgctga cgcggcggaa taatgccgcc acatcaacag
    19981 cagtcaaaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac gtttccacca
    20041 gtgcctgcat tcgcgcattt tgcgtcagaa tgttgtcagt aaaacgagcc actacttcag
    20101 gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt gggcttttta
    20161 gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc agcttcacgc
    20221 gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga tcgggaagag
    20281 gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca atggagcggt
    20341 tgatccacca aaccatgcct gcgccaatca ccagtgcgat ccccaacaaa atggcgctgg
    20401 cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg ttcggtttgc
    20461 ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca acatacatca
    20521 ccgaactttc gggatcggca ggattttgca acgtgctacg cgcaccatac tgaccacgca
    20581 acgttagcca gacgtcattc cagcgcgaat aatcctgccc aacggcttta tttgccgaat
    20641 cgaacaatac tttgccctgc gcatcggtca tatagacatg atattcattg cgcactttgt
    20701 taatgccacc gatattggcg cgaaacgggc gatgttgtag ctgattaaac gcctgagcca
    20761 gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc gccagcaacg
    20821 ttgcggtatc gattaacgtc ccctccgttg ctcttcgcac gcccggttta acttctttga
    20881 caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag cccagcagca
    20941 accgcatgcc gatacgcatt acaggcccct caggctatat cccatgccgc gatgagtatt
    21001 aatcggagaa agatcggggt tgatggcgcg cagcttggca cgcaacgttt taatgtgggt
    21061 atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca ttagttgctg
    21121 gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa actcataccg
    21181 cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat tcaattcaaa
    21241 atgaccaata cggatgacgg gagacggcga cgagaacttc ttcacccgac gcagtaaggt
    21301 gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt cgtcagcgcc
    21361 aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga acagtacagg
    21421 taacgccgga tggagcgcca gtaactggcg gcacaattca aagccgctga tatcaggcaa
    21481 accaacatcg agaatcatga cgtcgggaac ctgctggcga gctttatcca gcaccggcaa
    21541 gcctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga ccagcgtgtc
    21601 ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa atcgcctctg
    21661 ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg aaccttctac
    21721 aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat aaaagcgcac
    21781 gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct cgccctgagc
    21841 tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac aagaaatagc
    21901 cgcatcggag gtatcttccg ccagacccaa tcccccttta ataccacctg ttttcgcccg
    21961 gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca caacaatttt
    22021 gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt ctgcatgagc
    22081 caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt tcatattgtt
    22141 accattttta tctattacac gagcaattag ttcgtcacgg taaataacat attgattaag
    22201 aacaaaatct caatactttt atttccgtca agccctaaaa catactctac taagaaaaaa
    22261 acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt caggtgctcg
    22321 ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga ccttttaatc
    22381 tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc gaaagcaggt
    22441 tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc tattggcgcg
    22501 tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac tgcgcgtccg
    22561 attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac ccgcgcattc
    22621 aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg gagcgccttt
    22681 ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa atttgtcacc
    22741 ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct ggagcaaatc
    22801 tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg caacgcgccg
    22861 accattccgc cagtgctcta cggcctgaat gaaacgcgtc cgagtcagga taaagacgac
    22921 gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg ctatgtactg
    22981 acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta tgaaggtctg
    23041 ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc aatgctcaac
    23101 ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga cgccaaagcg
    23161 ggagatcgcc caattaatct gcgctgtgaa ctgctgattc ctatccgccg ttaacgctgc
    23221 agctcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc aacgacccag
    23281 ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca gttacgcaga
    23341 cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag acatcccagt
    23401 gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg gcaagattcc
    23461 agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg aatacgcccg
    23521 tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt cagagaatcg
    23581 atatgtcttt tctccagcac acccatgttt aattcacgca ggcgagaatc aaagatgatg
    23641 tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc taaatcgcta
    23701 ctgataatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat cgcctgttgc
    23761 tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc ggcgttccac
    23821 tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg tatactgctg
    23881 taacgttaat tttctgagta atgctgatta tgcaccaagt tatctgtgcg accaccaatc
    23941 ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa ggatcctgcc
    24001 atattgcatc cgttgccgtc gagagcggtg taccggaaca gccctttggc agtgaggaaa
    24061 cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca gaggctgatt
    24121 tttgggtggc gattgaagct ggcatcgatg gtgacagcac tttcagttgg gtggtgattg
    24181 aaaacaccag ccagcgcggc gaggcgcgtt cggctacttt gccgttacct gcggtgattc
    24241 tgcagaaagt gcgtgaaggt gaggcgcttg gtcccgtgat gtcgcgttat accggtattg
    24301 atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa ctcactcgcg
    24361 ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcacaat gccgtgtatc
    24421 aggcattgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc acgatgcctg
    24481 atgcgacgct gccgcgtctt atcaggccta caaaatcaat cgcttttcag caacacctct
    24541 tccagccact ggcgcagctc cacgggcgcg gctttcaggc tgttagatcc acgcgtaatc
    24601 gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct gactcatttc gccacgcaac
    24661 agctcttcga caatacgcac gcgagtcccc aacgcttcgc gctcatctgg cgtcagcatc
    24721 aggtttaaca acggtaaatg gagatcgttt tggtaggcat tcttaagcag gtcgacaaaa
    24781 cgtaaccact cctggtgacg ctgttctgcc atcgctgctg aatagggtga ttgttgggcc
    24841 ataatatgtc gccattgtta gcgggggaag caaaatgcct cccccggttg tactcgctaa
    24901 agagtacgat agcatatcat aaacgtgcgg atcagtaacg acgtccccat tccgtggcgc
    24961 tcatcaacgt cggtttatcc cccatgaaat aacggtagta agcgtcataa gccagcacgt
    25021 tcttcacata accgcgcgtc tcggagaacg gaatactctc gacaaatgcc acagcgtcga
    25081 tacgcccggc gctgttgcca agccaggttc gcacccgtcc aggtccggcg ttataagctg
    25141 ctgaggagaa aatacggtta ttgccaaact gctgataaac atattgcagg taactggtgc
    25201 caatgttgat attcgtttcc ggatccagca attgcccagg gctgctataa ccaggaatag
    25261 agaacatctt caccgtatgg gtcgctgtac caggcataat ctgcatcagg ccgctggccc
    25321 ctaccggtga tttcactttc ggattccagg cgctctcctg acgggcaatc gccatcgcat
    25381 agctttgcgg gatctctttc ccactggtgt aacgtttgaa aagatcgttg taagccagcg
    25441 ggaatcgctc ttccagatga tcccaaagct tcccggcgat cgttgcctga acgctaagat
    25501 cccaccattg gttattgaaa gcataccgag ccagttgagc ctgctctgtt tttgacttgc
    25561 tcttcaccag attggcccac tcgctacgcg cggtgttatc gagattccag tacatcaact
    25621 cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct gtcaacattc tgcggcgctt
    25681 tatcaatctt cagctcatac tcttctccga tgcgttgtgc tgcaaccatc gggtagaaac
    25741 cacgctgttg catgagttga tgcaaaatct ctttcgcttc agcttcacgt ccgcgttcca
    25801 gcaataaatc cacctgccag taacgccatt catctttctc ttttgcttcc atcggcagac
    25861 gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc aagtgccatt cgcacacggc
    25921 gttcaatcaa cgacgtggat tgcgagcgca taatgacgtc atcgcgccat ttcgcctgct
    25981 cgtcggtgac atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc tcctgaatct
    26041 gatcttcatt aagctgctgc acctgggcaa gcgacgggat catcagccgt gcattttcag
    26101 catcctgccg cgccacactg gcaaacgcca ccgctgccat ttgacgggta aaatcggtcg
    26161 ctccggttgt acgcgcgaag gtcagtaccg tattagggtt gttcgccagt gaaatgattg
    26221 ccgaggcgat agtctggtaa tcggcaggca tctgccctgc cagcactgtt accaggccag
    26281 tgttacccgc tttcatcgcc agacggatac gctctaaata cgccagcgga tcttgtttac
    26341 ctgacgcacg ccagacgcta aataacttgt cacaggcgtt aggctggctc ttgccggtta
    26401 gccacagctc tttcgcccct tgccaggctt cttcactctg cccggtgttc catttcgcat
    26461 agtagtaatt acattgcgct tcggtagttc cgggcttttc cgggctaaag gctaacaagc
    26521 cacgccagtc ttcacgccgc gccagttcat tgacgaaacg agattgcagc gtgcgagcgg
    26581 gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt caccgccggt tgattcatca
    26641 gatcatcggt gatctggcgg tattccaggt agggataaag cggataatcc ttcagtccag
    26701 gcatcatttg ttcgaccaca tccatttgtc gattatccca ggcctgcttg atttgcgcgt
    26761 aacggctacg ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc gtcagcagac
    26821 agacaccggc agccaacagc cgccaggtaa cttgtttggc tttttccaca agcgcatcct
    26881 ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat agatttatgc taaccagtca
    26941 ttgccgttta cgccacgtta cggacacttt tttactttta ctgcgaaggt gatcgaccaa
    27001 ctgggattag gcggggaaaa aggctacact tcgcctttga aaactcatca tcacgataaa
    27061 catagaggcg aagtccaacg tggctcaatt cgtttatacc atgcatcgtg tcggcaaagt
    27121 tgttccgccg aaacgtcata ttttgaaaaa catctctctg agtttcttcc ctggggcaaa
    27181 aattggtgtc ctgggtctga acggcgcggg taagtctacc ctgctgcgca ttatggcggg
    27241 cattgataaa gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga ttggttacct
    27301 gccgcaggaa ccgcagctga acccggaaca caccgtgcgt gagtccattg aagaagcggt
    27361 ttctgaagtg gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt acgccgatcc
    27421 ggatgccgat tttgacaagc tggccgctga acaaggccgt ctggaagaga tcattcaggc
    27481 tcacgacggt cataacctga acgtacagct ggagcgtgcg gcggatgcgc tacgtctgcc
    27541 ggactgggac gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg tagcgttgtg
    27601 ccgcctgctg ctggaaaaac cagacatgct gctgctcgac gaaccgacca accacctgga
    27661 tgccgaatcc gtggcctggc tggaacgctt cctgcacgac ttcgagggca ccgtggtggc
    27721 gattacccac gaccgttact tcctcgataa cgttgcaggc tggatcctcg aacttgaccg
    27781 cggtgaaggt attccgtggg aaggcaacta ctcctcctgg ctggagcaga aagatcagcg
    27841 cctggcgcag gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga aagagctgga
    27901 gtgggtacgt cagggaacta aaggccgcca gtcgaaaggt aaagcacgtc tggcacgctt
    27961 tgaagagctg aacagcaccg aatatcagaa acgtaacgaa accaacgaac tgtttattcc
    28021 acctggaccg cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta aatcctacgg
    28081 tgatcgcctg ctgattgatg acctgagctt ctcgatcccg aaaggggcaa tcgtcgggat
    28141 catcggtccg aacggcgcgg gtaaatcgac cctgttccgt atgatctctg gtcaggaaca
    28201 gccggacagc ggcaccatca ctttaggtga aacggtgaaa ctggcgtcgg ttgatcagtt
    28261 ccgtgactca atggataaca gcaaaaccgt ttgggaagaa gtttccggcg ggctggatat
    28321 tatgaagatc ggcaacaccg agatgccaag ccgcgcctac gttggccgct ttaactttaa
    28381 aggggttgat cagggtaaac gcgttggtga actttccggt ggtgagcgcg gtcgtctgca
    28441 tctggcgaag ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac caaccaacga
    28501 cctggatatc gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc cgggctgtgc
    28561 gatggttatc tcgcacgacc gttggttcct cgaccgtatc gccacgcaca tcctggacta
    28621 ccaggatgaa ggtaaagttg agttcttcga aggtaacttt actgagtacg aagagtacaa
    28681 gaaacgcacg ctgggcgcag acgcactgga gccgaagcgt atcaagtaca agcgtattgc
    28741 gaagtaatgc gtaaaatgcc ggatgcggcg tgaacgcctt atccggccta caaaccaaga
    28801 aatttcaata cgttgtgaat ctccaggcct gataagacgc ggtaagcgtc gcatcaggca
    28861 ttgagcaccg aatgccggat acggcgtgaa cgccttttcc ggcctacaac agccttagta
    28921 tccgcaattt cgtagcgggt cacccgtgca ggttttttca aaccttgttt aatctcgaca
    28981 gcctcttcca gagaggtttt caactcatca aaaaacttat tttatacctc gcctttgagc
    29041 cgtttcatcg cggttatctc tgctccccca tcatctcccg caccagctcc acgcagcgca
    29101 ggaaacggct gtcataatcg tcctcttcaa cccgcacgaa ttcgatattg ttctcttcca
    29161 gcatctccac cagcaagttc tggaactctt tgcgatccac cgaactgccg aggctgcgta
    29221 aaccatccgc cacccacggc gtgttgttct ccagcaggat caccagatcg aaacggtatt
    29281 catcaatcaa cgcctgtacg aacggatgct cacgcccttc gtactttttg cagaacgcct
    29341 gggtagtgac aaaatcagtg tcaataaacg ccactttatt ggcatatttc actgcaaaat
    29401 caatgtattg tgcgtggccc agcgcgattt tatcgtaatc ggaatactgc aatgcgatct
    29461 catcaccgcc gaggtgtgaa aagacataat cgcgaccata ttcccacgca ctggtggtat
    29521 tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg ccaccaagga
    29581 tcgccacggt acgtacaaag aacggcttca cttccgtagg aatatattcc cagtagcgga
    29641 acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc ggatcgacca
    29701 gcaccgtatc gatccccaga tgttccatat actgtggcgc atcggcttct tccgaggtgt
    29761 agatcaggtc cggctgaatc cccttttcag ccataaactt tttgatgccg ttgctccaca
    29821 catcccagcc gtgcggatac ggctccatgc cctcttcgtt gaaagcatga atgcgaatat
    29881 ttttctgata tttaaaagtt tgtaataacc agcgcagacg atccggcacc gtgggctgct
    29941 gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt atcgtcaaaa cccataatga
    30001 tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt ccggtatgca
    30061 gcgggtagaa cttaccgaat acgacaccaa tggttttctt ctgtcgggga aattcaagcc
    30121 ccaaaaaacg gtgcaacgcc tccagctttt gcgcgctggg gcttttaatt ttggcattca
    30181 gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc tgtagcgtgc
    30241 agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct ccctccgtat
    30301 ctctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa cgccaaaaat
    30361 ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg cccggcgaaa
    30421 accgtgtttc gccgcttctg aaattcgttc ctgaccgctg ggcaccgggc ggatctcccc
    30481 tgccagcccg acttcaccaa acaccaccag atcctgtggc agcggtctgt cacgcaggct
    30541 ggaaaccatc gccagcagta acgctaaatc ggcactggtt tcggttacct tcacgccgcc
    30601 gaccacgttc acaaatacat cctgatcggc catttgcaga ccaccgtgac ggtgcaagac
    30661 agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc gcggattcgc
    30721 catcatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg ttccttccca
    30781 caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga aaattgccga
    30841 agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca gctcattcac
    30901 cgcgccgaag cggtttttat ggctgcgcaa ggtgcgaaaa cgggagtcgg catcaccatc
    30961 cagaagcacc gaacagtcga tacagtgttc cagcacttta gggccagcca gcgagccatc
    31021 tttggttacg tgcccaacca tgacaatcgc cacaccgcgc gttttggcga agcgcgtcag
    31081 ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgattgta catccgccat
    31141 atgcatcacc tggatcgagt caattaccat cagtttcggt tgctcttctt cggcaatcag
    31201 gcagatctgt tcgatgctgg tttccgacaa catattgaga ttgtcagtcg gcaggccaag
    31261 gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccagtgacat acagcgtttt
    31321 catctgctgg gccagtttgc acagcgtttg cagtagcagc gtggatttcc ccgcacctgg
    31381 gttaccgcca atcagaatgg cgcttcccgg caccacgccg ccgcctagta cgcggtcgaa
    31441 ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg agagtttctg
    31501 gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac gcgccaccgt
    31561 tggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg cactgcactg
    31621 cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc gttttggagc
    31681 ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatggcggg cgagcaatta
    31741 cttctgattc aggctgccag agaggatgca gaataccccc atcaggtcag cgtgacggat
    31801 ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc ccagccctgc
    31861 tgctttgatc atcggcaggt cattagctcc atcgccaatc gccacggtct gcgccagcgg
    31921 gatttcatac tcctgcgcga ggcgagtcaa agttttcgct ttgtactgcg cgtctacgat
    31981 gtcgccaatc acattgccgg taaatttacc gtccatgatc tccagttcat tggctaccac
    32041 ggcggtcagg cgcagcttgt cgcgcagata ttcagcaaag aaagtaaagc cgccggaggc
    32101 aatcgccact ttccagccca gcgtttccag cttgagcacc agttgcgtta agcctggcat
    32161 cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt tcagcgtcgc
    32221 cacacggctg cgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc gttcggttac
    32281 ttccgccacc atctcgcccg ttccggccag tttggcaatt tcatcaatac attcaatctg
    32341 gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt gcgggatttt
    32401 ccccagcggg gcgacatcca gttgcgcttc atgggccagg cgcgtagccc gtgcggtgag
    32461 tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa caatcaccat
    32521 cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc cacgaccata
    32581 cagcagccag ccgctacgac ctgcgtggta atccagtggc atcacttcat caccacttaa
    32641 tgaaagaggc agacccggcc ataaagagac atcttcaggc aggtcgcacc aggtaatgtt
    32701 aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg aggctacctt
    32761 gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa gatggctcgc
    32821 acaaaactga aattccggct gcatcgggca gtgattgtcc tgttctgtct tgccttgtta
    32881 gtggcactga tgcagggggc gtcatggttt agtcaaaacc accagcgaca gcgtaatcca
    32941 cagctggaag aactggcccg caccctggcg cgtcaggtga cgctgaacgt tgcaccgctg
    33001 atgcgtaccg actcaccaga tgaaaaacgc attcaggcga tcctcgatca gttaacggat
    33061 gaaagccgca tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat cgcccgttca
    33121 ggcgaaagcg tcgaagtgcg cgaccggcta gcgctcgacg gtaaaaaagc aggcggctat
    33181 tttaaccagc agattgtcga gccaattgcg ggtaaaaacg gaccgctcgg ctatctgcgc
    33241 ctgacactcg acacccatac gctcgccacc gaagcccaac aggtggataa caccactaac
    33301 attttacgcc tgatgttgct gctctcactg gcaatcggcg tagtgctgac ccgcacgctg
    33361 ctacagggta aacgcacccg ctggcagcaa tcgcccttcc tgttaaccgc cagcaaaccg
    33421 gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca ttactacaag aaaggaaatc
    33481 gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt taacctggcg
    33541 gtggaagagt gtatttttcg ccaaatgccc gccacgcagc gcgttctgtt tctctggcgc
    33601 aatgccgaca cggtagtgat tggtcgcgcg cagaacccgt ggaaagagtg taatacccgg
    33661 cggatggaag aagataacgt ccgcctggcg cggcgcagta gcggtggcgg cgcggtgttc
    33721 cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta cgataaaacc
    33781 atctctacgt cgattgtgct caatgcgctg aacgcgctcg gcgtcagcgc cgaagcgtcc
    33841 ggacgtaacg atctggtggt gaaaacagtc gaaggcgacc gcaaagtctc aggctcggcc
    33901 tatcgcgaaa ccaaagatcg tggcttccac cacggcactt tgctgctcaa tgccgacctt
    33961 agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa aggcattacc
    34021 tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggatcac ccatgagcag
    34081 gtttgcgagg ccataaccaa ggcctttttc gcccattatg gcgagcgtgt agaagcggaa
    34141 atcatctccc cggacaaaac gccagacttg ccaaacttcg ccgaaacctt tgcccgtcag
    34201 agtagctggg aatggaactt cggtcaggct ccggcattct cgcatctgct ggatgaacgc
    34261 tttagctggg gcggcgtgga actgcatttc gacgttgaaa aaggccatat cacccgcgcc
    34321 caggtgttta ccgacagcct caaccccgcg ccgctggaag ccctcgccgg gcgactgcaa
    34381 ggctgcctgt accgcgcgga tatgctgcaa caagagtgcg aagcgctgtt ggttgacttc
    34441 ccggaccagg aaaaagagct acgggagttg tcgacgtgga tagcgggggc ggtaaggtaa
    34501 ctcgctgatt aaaataccaa aggaagggaa tatgatcgat ctggaaaacc aggagcggga
    34561 gattatcaat ttaatgttaa gccagcgtat ttcctggcta gccgccgtgc gtatcaggca
    34621 taaattatca cttgctgaag taagtaaaat gttaggtatc tcgattaatt ctctaaaata
    34681 gatcgaaaag acggaacggc ttagctcaaa catcaaaagc aaaatgggag aaatttatgg
    34741 ttgtccacca gagttgctga tatgcccttc ctggatgacg gctgaacata aatgaataga
    34801 ttcgtgaata gatacttgaa tgtattttat tttttatcca tatgaaaaat aatagtaaat
    34861 ctataaatca gttgctattg atgaatagat actaaaaagc cggagcagtc ccccactccg
    34921 gcttaacacg acaatcgcct ttcagcgaaa cacaattact ctttatcgcc cagcagaacg
    34981 gattccagtg cgattttgat catgtcgttg aaggtggtct gacgctcagc ggcagtggtc
    35041 tgctcgtgag tgcggatgtg gtcagacaca gtgcagatgg tcagggcttt cgcgccaaat
    35101 tctgcagcga cgccgtagat accagccgct tccatttcca cgccgaggat gccgtatttt
    35161 tccatcacgt cgaacatttc gccgtccgga gagtagaaca ggtcagcgga gaacaggtta
    35221 ccaacgcgag catcaatgcc cagtgcttta gccgcgtcta ccgcgttacg caccatgtca
    35281 aagtcagcga tagcggcaaa gtcatgatct ttaaaacgga tgcggttaac tttggaatcg
    35341 gtgcaggcac ccataccgat aacgacgtcg cgcagtttta cgtgcggcag aactgcgcca
    35401 caggaaccca cgcggataat tttcttcacg ccgaaatcgg tgatcagttc tttggtgtag
    35461 atggagcagg acgggatacc cataccgtga cccattacgg aaattttgcg gcctttgtaa
    35521 gtaccggtga agcccagcat accgcgaacg ttgttcactt cacgggcatc ttcaaggaaa
    35581 gtttcagcaa tatacttcgc acgcagcggg tcgcctggca tcaaaactac gtcagcgaaa
    35641 tcgcccattt ctgcattaat gtgtggggta gccattgttt tatccttatc aacatgtttt
    35701 caccctctcc ctgagggaga gggccggggt gagggcatca agccgcaccc attatcagaa
    35761 catggctttg ccatattcca tatcagaagt accaaaatat tttgccagag tctggccgat
    35821 atccgcgaag gtttcacggt gacccagtga gcccggtttt actttcgggc cgtaaaccag
    35881 taccggaatg tgttcacgcg tgtggtcagt accggtccag gtcggatcac aaccgtggtc
    35941 agcagtgagg atcaggatgt cgtcatcgcg cagcagagac atcagctccg gcagacggcg
    36001 gtcgaacagc tccagaccca cagcataacc ggcgacgtcg cgacggtggc cccaggaaga
    36061 gtcgaagtca acgaagttgg tgaagacgat ggtgttatca cccgcttctt tcatctcttt
    36121 gatggtggcg tcaaacagcg cgtccaggcc agttgctttc actttcttgg tgataccgca
    36181 gttggcgtag atgtctgcaa ttttaccgac agaaaccacc tgaccgtgtt tttcatcaac
    36241 cagtttctgc agcacggtcg gtgctggcgg ctcaacagcc aggtcgtgac ggttaccggt
    36301 acgctggaag ttaccggctt tgtcaccgat aaacggacga gcgataacac gaccgatgtt
    36361 gtagccgccg ttggtcagct cttcacgggc gatttcgcac agttcgtaga gtttatccag
    36421 gccaaaggtt tcttcgtggc aggcaatctg gaacacagag tcagcggagg tatagaaaat
    36481 cggcttgccg gttttcatgt gctcttcgcc cagctgatcc agaatgaccg taccggaaga
    36541 gtggcagtta ccgaggtaac ccggcagatt agcgcgttca accagtttat ccagcagctc
    36601 ttgcgggaag ctgttttcgt gatcggagaa atatccccac tcaaatagaa ccgggacacc
    36661 ggctatttcc cagtgaccag acggggtatc tttaccggat gacatttcgt gcgcccatgc
    36721 gtacgcgccg ataacttcag cgttgccgtc cattcccgcc ggaatgaaac cggtagaacc
    36781 ttcgtgtgct ttcgccagcc ccagacgggt cagatttggc agattgagcg gacctttacg
    36841 accgttatca gcttcgcctt tggcacaagc ttctgcgata tgacccaggg tgtcagcccc
    36901 gacgtcacca aagcgttctg catcttctgt agcgccgatg ccgaatgagt ccagcaccat
    36961 aataaatgca cgtttcatat gttctccgta ctcagtgctt caaatttaaa tgatcagatc
    37021 agtataccgt tattcgctga tacggcgata gacagttggt gtgctttccg gtgctttatc
    37081 ggcaagttta attgccgctt tcaccgcttt cgccgcttcc tgccagctgt tttcgtcttt
    37141 cgcgtggata accgccagcg gacgctgacc gtctacctgg tcgcccaaac gcgccatatc
    37201 agtaaagccg acgctgtaat caatggtgtc agatgcctga cgacgaccgc cgcccattgc
    37261 aaccactgcc atccccagcg cgcgggtatc catttcactg acaaaacctt cggtatcagc
    37321 atagactgct ttcgtcagca tcgctgtcgg cagatacttc gcgtagttct caacgaagtc
    37381 ggttgggcct ttttgtgccg ctaccatacg accaaagact tctgccgctt taccgttgtc
    37441 cagcactgct tgcaatttcg cgcgcgcttc ggcgtcatct ttcgccagtt tgccggagat
    37501 aagcatctcc acgcacagcg ccatcgtgac atcaaacaga cgcgggttac gatactcacc
    37561 cgtcaggaac tgcaccgctt cacgaacttc aaccgcgtta cctgcactgg aggccagtac
    37621 ctgattcatg tcggtgagca gcgcggtggt gcgcacgcca gcgccgttag ccacgccaac
    37681 aatcgcttcg gcaagggctt cagagagttc gtaggtcggc ataaacgcgc cgctacccac
    37741 tttcacgtcc atcaccagcg cgtccagacc ttccgcaagt ttcttcgcca gaatagaggc
    37801 ggtgatcagc gggatggagt ccacggttgc ggtaatatca cgggtcgcgt agaaacgttt
    37861 atccgccgga gccagtgagc tggtctgacc gataatcgcc acgccgacgt ctttaataat
    37921 ttcgcggaaa cggttgtcat ccgggaaaat gtcgaagcca gggatggatt ccagtttgtc
    37981 gagcgtaccg ccagtatgac cgaggccgcg accagagatc atcggaatat agccgccgca
    38041 ggctgcgacc atcggcccca acatcagcga agtcacatcg ccgacgccac cagtggaatg
    38101 tttatcaaca atcgggccat tcagatgcag gcttttccag tcgagaacgg ttcctgaatc
    38161 tcgcatcgcc atggtcagcg agacacgctc aggcattgtc atatcgtgga agaaaatggt
    38221 catcgcgagg gcggcaatct gcccttcgga gatagtgttg tcgcgaatgc cgttgataaa
    38281 gaaacgaatt tcttcatcgc tcagtgcatg accatcacgt tttttacgaa taatttcttg
    38341 tgcgagaaac aaggtaacct cccgaggaaa agagtatgag gcggcttttg ccggatgcgt
    38401 ctacgcctta tccggcctac gggtagatac gtctcgtagg cctgataagc gcagcgcatc
    38461 aggcgtaaag catcttactt agtagctgct ggcgctctta ccgtcaccgt ggcccagcgc
    38521 tttcaacagg cttgccagca ggctggaagc accaaagcgg tagtgacgcg catctgccca
    38581 gtcagcaccg aacagttcat ctgcgatggc gagatatttc tgcgcatctt ccgcagtacg
    38641 cacgccgccc gctggtttga aaccaacggt tttttctacg cccatatcac ggatcacttc
    38701 catcatgatg cgcgcgcttt ccggcgtcgc gttcacagcc actttaccgg tagaggtttt
    38761 gatgaagtcc gcacccgctt tgatggagat ttcagacgct ttacggatca gcgcttcgtc
    38821 tttcagttcg ccggtttcga tgatcacttt cagcagtaca ttcgctgccg cgcaagcctc
    38881 tttacaggct ttcaccaggt caaaaccaac ctgctcgtta cccgccatca gcgcgcggta
    38941 cgggaacaca acgtcaactt catcagcacc gtaggcgatt gccgcacggg tttctgccag
    39001 cgcgatttcg atgtcatcgt taccgtgtgg gaagttggtt accgtagcga ttcggatttc
    39061 cggggtgccc tgctctttca gcgttttgcg agcaatcggg ataaagcgag gatagataca
    39121 gatagcggcg gtattgccga ccggggtttt ggcctgatga cacagagcaa ttactttctc
    39181 gtcggtgtcg tcgtcattca gggtggtcag gtccatcaat ttcagtgcac gcaggctgct
    39241 tgctttcaga tcagtcattt cattctcctg gcttgtcgcc aataaaattc accttgcgag
    39301 tttgttagta ttctaacatc tactccgcaa cacacttcga tatacatcac aattaaggaa
    39361 atctacttac aagtttgcat cactgtaatg cgatctggtt caaataattc actttcaaat
    39421 gaatgcgtca gtggtggcaa acgcatcagg atcaaagtga acatcacgaa acttcttaca
    39481 atggcgcatc accttcggca taaaaggaaa agaagatgcc cacttctcat gaaaatgcac
    39541 tgcaacaacg ttgccagcaa attgtcacca gcccagtgct tagcccggag cagaagcgcc
    39601 attttctggc actggaagca gaaaacaatc tgccttatcc gcagcttcct gccgaagccc
    39661 gccgcgcgct ggatgagggt gtaatctgcg atatgtttga aggtcatgcg ccgtacaaac
    39721 cgcgctatgt cttacccgat tacgcccgtt ttctggcgaa cggttccgaa tggctggagc
    39781 tggaaggcgc gaaagatctt gatgacgcac tctctctgct gaccattctt taccaccacg
    39841 taccgtcggt cacatcgatg ccggtctacc tggggcaact ggatgcgttg ttgcaaccat
    39901 atgttagaat tctaacacaa gacgagatcg atgttcgaat aaaacgtttc tggcgttacc
    39961 tcgacagaac cctgccagac gcctttatgc acgccaatat cggcccgtct gattcgccca
    40021 ttacccgcgc gatcttacgt gcagacgcag agttgaagca ggtttcaccg aacctgacct
    40081 ttatctacga tcctgaaatc acccctgatg acctgctgct ggaagtggcg aagaacatct
    40141 gtgaatgtag caaaccgcac atcgccaacg gtccggtgca tgataaaatt ttcacaaaag
    40201 ggggctacgg gattgtgagc tgttacaact cactgccgct ggcgggtggt ggcagcacgc
    40261 tggtacgcct taacctgaaa gccattgccg agcgcagcga atcgctggat gacttcttta
    40321 cgcgcactct accgcactac tgccagcagc agatcgccat catcgatgcg cggtgtgaat
    40381 tcctctatca acaatcacac ttctttgaga atagcttcct ggtgaaagaa gggctgatta
    40441 accctgaacg ttttgtgcca atgtttggca tgtatgggct ggcggaagcg gttaacttgc
    40501 tgtgtaagaa agaagggatt gccgcgcgct acggtaaaga agccgccgca aatgaagtgg
    40561 gttatcgcat cagcgcgcaa ctggcggagt ttgtcgccaa tacccccgtg aaatatggct
    40621 ggcaaaaacg cgccatgtta cacgcacagt cggggagcag ttccgatatc ggcaccacgc
    40681 cgggcgcgcg tttgccgtat ggtgatgagc cagatccgat cacccatctg caaactgtcg
    40741 ccccgcatca tgcttattat tattccggca tcagcgacat tctgacgctc gacgaaacca
    40801 tcaaacgtaa tccgcaggcg ctggtacagc tttgcctcgg tgcctttaaa gccggaatgc
    40861 gtgaatttac tgccaatgtc agcggtaacg atctggttcg cgttaccggt tatatggtgc
    40921 gtttgtcgga tttagaaaaa tatcgcgccg aaggttcacg caccaacacc acctggctgg
    40981 gcgaagaagc cgcacgcaac actcgtattc tggaacgcca gccgcgcgtg ataagccatg
    41041 aacagcagat gcgctttagt cagtaagatt atccccttct cctgcgttga cgggccaggc
    41101 agtcgtctgg ctctgttttt gcagggctgc aatctgcgct gcaaaaactg tcacaatccg
    41161 tggacgatgg gacgttgcaa tgactgtggg gagtgcgtgc cacagtgtcc gcatcaggcg
    41221 ttgcagattg ttgacggcaa agtggtgtgg aacgctgtgg tttgcgagca gtgtgatacc
    41281 tgcctgaaga ggtgtccgca acatgccacg ccaatggcgc aatccatgag cgtggacgaa
    41341 gtgcttagcc atgtccgcaa agcggtgctg tttattgaag ggataacggt aagcggcggc
    41401 gaagccacga cccaactgcc gtttgtagtg gcgctgttta ctgctatcaa aaacgatccg
    41461 caactgcgcc acctcacctg cctggtggac agtaacggca tgttgagcga aaccggctgg
    41521 gaaaaattac tcccggtgtg tgacggcgca atgctcgatc tcaaagcgtg ggggagcgaa
    41581 tgtcatcaac acttaaccgg acgcgataat cagcagatta agcgcagcat ctgtttgctt
    41641 gcagagcgcg gcaagctggc ggaactgcgt ttgctggtaa ttccagacca ggtggattat
    41701 ttgcatcaca tcgatgagct ggcgacgttt atcaagagac ttggcgatgt tccggttcgc
    41761 ctgaatgcgt ttcatgccca cggcgtgtat ggcgaggcgc aaagctgggc gagcgccacg
    41821 ccggaagacg ttgagccgtt ggctgatgcg ttaaaggtgc gcggggtgag ccggttgata
    41881 tttccggcgc tctatttgtg acatgccgga tacggcctac ggttcggcac agacttgtag
    41941 gcatgataag acgcgacaag cgtcgcatca ggcattatgc gccgactgcc ggatgcggcg
    42001 tgaacgcctt atccggccta cggttcggca cagacttgta ggcatgataa gacgcgtcag
    42061 cgtcgcatca ggcattgtgc gccaactgcc ggatgcggcg tgaacgcctt atccggccta
    42121 cggttcggca cagacttgta ggcatgataa gacgcgtcaa gcgtcgcatc aggcattgtg
    42181 cgccaactgc cggatgcggc gtgaacgcct tatccggcct acggttcggc acaaaccagt
    42241 aggcatgata agacgcgtca agcgtcgcat caggcattgt gcgccaactg ccggatgcgg
    42301 cgtgaacgcc ttatccggcc tacggttcgg cacaaacctg taggcatgat aagacgcgtt
    42361 aagcgtcgca tcaggcatta tgcgccgact gccggatgct gcctacgaag ggtaggctac
    42421 ccactaacgc taaacaacgc atacgtatta ttaagcaaca cttcggcaat ctcatcttct
    42481 ggttccgggc gcaactcgca aagcacgtca aacacccgca cagcctgttc cggtcggtta
    42541 ggctgcccct gaaagccgtt gagcggcata tccggcgcgt cggtttccag caacaacgat
    42601 gccagcggta attttgcgat gacatcgcgg gttttactgg cgcgtggata ggtgatagta
    42661 ccgcctacgc cgattttgta gcccagctgt acaaaccgct cggcctgttg caggctgccg
    42721 gaaaaaccgt gaaccacgcc agtgcacggt aaatcgtgac gtttaagatg catcgccagt
    42781 ttgtcgtgta tacgccggga atgaaggatc accggcagat cgtagcgttt cgccagtttc
    42841 agctgttcgt cgagtaacca ctgctgcctc tcaaattgcg gatcgtcgcc gaagagatcc
    42901 agaccgatct cccccaccgc caccaccttc gccggacgcc tttccagcgc ctgctgtagc
    42961 tgatcaagag acacatcgct atgtttttcc aacatccccg gatgcaaccc caatgcggca
    43021 tacagcggtt gatatttttc cgctaacgcc tgcacgcggg caaaattttc cgcctctgtc
    43081 gccggaacaa tgatcttgcc tacgcccgct tgtgccgcgc gttgcaggct ggcctcttca
    43141 tcgccactaa acggcgggaa atcaaaatgg cagtgggtat cgataaaacg gcaaatcaag
    43201 ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg agcatcgctc acttcggcaa
    43261 ccagcgtatc gttggcgact ggcgcaggcg gaataacgat cgattccggc gtcaccactg
    43321 gcaccagatg gcgggtaagc ggcgcttttt cagttaatag cttgccaacc gtggcgagga
    43381 aataacgacc gcataaacgc ccaagtttat agtcttcacg cagcgccgga atccgactgc
    43441 caagcgcgat actatgtaat ggcttcggcg gataaatttc gaatatccgc agcttgcccg
    43501 gtggtttctc aataaattgc tgaatgtcac gatagctggt ttcatgatgc tgcaccagat
    43561 tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc catgcgtttg aaccactgcg
    43621 gcgtgtagta catttgtgac ggcacagtgc gaatgacgac caacgtttta gcgccctgcc
    43681 ttgccgcctc tttaaccgga atcgcatcac tgatcccgcc atccaggtag ttaatgcctt
    43741 ccagcgacac tccgctacga taaaagccag gtatcgcact ggaggcgcga atcacatcca
    43801 gccagttttg tttggttggt aaaaagtaat taggcgcgta gtcatcctga cgacaggcgc
    43861 acatataaaa cgatttgccg ctgtcaaaca accgcgcggc ggtatccatt tgcaacggca
    43921 tctggcttgc ggtggcctcc accagccagt cgagatcgat aagatttcct ccacggacaa
    43981 agcgcaatgg atcgaaaaat tcgcgttttg tggtatagcg catgatgact ttgcgcgcgt
    44041 aaccgggctg attgcagata aacgccgaga ggttctgcgc cccggcagat gtgccgagat
    44101 aaagatcgaa aggattaaac tgcgcgcgca taaactcatc cagcacgcca gccgtgaaaa
    44161 ttccacgctg tccgccgcct tcgcacacca gagctattcg tccaggctgg aacggcctta
    44221 gcgacaacgg cgcaatatta ccaagcgtta caggtattcg ctgccccacc tctgccttcc
    44281 tgtttttgtt gtgaaagaat gacaaagtaa cgcaatttat tattcgctaa aaccgcaaaa
    44341 gccagtccgc tggactggct ttggataaaa ttttcttaat ggtctgaaat ctagggtcgt
    44401 tttcggccca tgaacaaact caccaggaac agaataatcc cgacgacaaa gacaatttta
    44461 gctgcgcctg cagcggtacc ggccagacca ccaaacccaa gtgcggcggc gattaacgcg
    44521 ataaccagaa atatgatgcc ccaacgaaac ataagcgtct cctttaccat agttaatgtc
    44581 accgcttgat atgagcgtga aaatcactca atgcgatatt tttagtgtgg tgcacatcgc
    44641 gcctcccgac aaagttcggg aggacgaatt acgacgaatt acttagtttt cagatcattt
    44701 ttaacgcttt tcacaccatc taccgctttg gcgatacttt cagcacggtc gctctgtgcc
    44761 tgggaatcga cggtaccgga gagctgaacc acgccgtcgg tggtttcaac tttcacatga
    44821 cgggaaggga cgatatcgtc cgccagcagt ttggctttga tttcactggt ggtggcggtg
    44881 tcacccgcgt agcccttcac agagccttct ttagcgtcgc gaacgtgcag tttgtcgctg
    44941 acagaggtca ccccttcaac gcctttcgcc actttcactg cctcttcggc ctgagcctgg
    45001 ctttcaacga aaccgctcag ggtcacgact ttttgatcgg ttttgacaga gatatcggtg
    45061 ctcttgatgt tgtcatgatc caccagcgcc gccttcactt tcgcggtgat ggcgctgtca
    45121 tccatgaaat taccgacttt attcatagag ctatcgactt tttgccctgc gctttcattg
    45181 gtagtctgtg cgttgttttc cgcgtaggca gagccggtcg cgacggcaga ggtcaacatt
    45241 acagccagca gagttttcga aatcttcagt cttgtcatag tcatcgattt attcctgtat
    45301 gtttgctcgt aatttgagcc tggcaacacg aggttgcatt gctgaatagg gagagacttc
    45361 accctctaca gaagtcaatg gtcgccatca caaaagcgat gggtgatgaa taacgaccat
    45421 tacagcctct gaatcagtta ttaatatcgg tagaatgaca atcgacggct ttagatactg
    45481 atatctacgc attgaacggt atttaacgcc gtcagaaatg tcattacttt gttaaatata
    45541 gatcacaatt ttgaaaccgc tcgggatatc agcgaaaaca taagcaaaag tgaatgtttt
    45601 aagaacattc cgtaagcggc taataaggaa gggaaattga cagggcgcag cggctaccgc
    45661 gccctggagg caagaattaa tgctcgcggg tctggtggaa ctgaacgtcg ggataacgtt
    45721 cctgcgccag gcgcaggttg accatgctgg tagcgatgta agcgaggtta tcgccgccat
    45781 caagcgccag ttggctttcg ttcttacgct tgaactcttc gaatttcttc gcgtccgcac
    45841 attctaccca gcgggcagtg gcaacgttga ctgattcata tactgcttca acgctgtatt
    45901 cgctcttcag gcgcgatacc accacatcaa actgcagcac accaacagca ccaacgatca
    45961 aatcgttgtt ggagatcgga cggaacacct gcaccgcgcc ctcttcggaa agctgtacca
    46021 gccctttgag cagctgtttt tgcttcagcg gatctttcag gcggatacga cggaacagtt
    46081 ctggtgcgaa gttcggaata ccggtgaact tcatcatctc accctgggta aaggtgtcgc
    46141 cgatctgaat agtgccgtgg ttgtgcaggc cgaggatatc gcccggatac gcttcttcaa
    46201 cgtgcgaacg gtcgcccgcc ataaaggtca gcgcgtcgga gataaccaca tctttcgcag
    46261 tacgcacctg gcgcagcttc atgccttttt catatttacc ggatactacg cgcataaacg
    46321 ccacgcggtc gcggtgtttc gggtccatgt tggcctgaat tttaaatacg aagccggtaa
    46381 atttgtcttc gctcgcctct acggtacggg tatcagtctg acgcggcatc ggcgcaggag
    46441 cccactccac caggccatcc agcatatgat caacgccgaa gttacccagc gcagtaccga
    46501 agaataccgg agtgatttcg cccgcaagga acaattcttt gtcgaactcg ttagacgcgc
    46561 ctttcaccag ttccagttcg tcacgtagct gctgtgccag atcttcacca accgcagcgt
    46621 cgagatccgg gttattcagc cctttaacaa tacggacttc ctgaatcgtg tggcctttac
    46681 cgctctgata gagataggtt tcgtctttat aaaggtggta aacgccttta aacagcttgc
    46741 cgcagccaat cggccaggtg atcggtgcgc agccgatttt cagctcgttc tcaacttcat
    46801 cgagcagttc catcgggtcg cggatatcac ggtcaagttt gttcataaag gtgaggatcg
    46861 gcgtgtcgcg cagacgggta acttccatca gcttacgggt acgatcttca acaccttttg
    46921 cagcgtcgat aaccatcagg cagcagtcca ccgccgtcag ggtacgatag gtatcttccg
    46981 agaagtcttc gtgccccggg gtgtcgagca ggttaaccag gcaatcgtga tacggaaact
    47041 gcatcacaga cgtagtaatg gagatcccac gctgcttttc catctccatc cagtccgact
    47101 tagcgtgctg gttggaacca cggcctttta ctgtaccggc ggtctgaatg gcctgtccga
    47161 acagcagcac cttctcggtg atggtagtct taccggcatc cgggtgagaa ataatggcaa
    47221 aagtgcggcg cttcgccacc tcttgcaaat aaggagacaa cgtcataatt ctttcttctt
    47281 gagtaagcgg catcgcgccg cgcttgttga aaacgaaaaa ttgcggctat tttacccatc
    47341 aacggggggg aggcaatcag tgtttacaca ggagctgctc cagttcgtgc aacgaagaaa
    47401 cggtccaggt gggcgcgatg ccttctggtt gctcgcgatt gtgcgcattc agccagcagg
    47461 tcgcaagccc ggcgttgatg ccaccgagaa tatcggactc ggcagtgtcg ccaaccatca
    47521 gcacgcgtga acggtcagga ttgcccgcct gttccagcgc ataatcgaaa attttcttat
    47581 tcggtttggc aacgccaact tcttcggaaa tcaccagcaa atcgaagtaa tcacgcaggc
    47641 ccgtgcgttc cagacgcact tgctgcaagg cgctaaagcc gttggtgatg atgccgattt
    47701 tggcgttgcc acgaatggcg ttaagcagag aaaccgcgcc cggcagcggc gtgcagattt
    47761 ccgccatcgc attaataaag gcatcgttaa gcaagcctgc ttcaactttc agccgttcgg
    47821 cccagctctc aaaacgcccg tgctgaagct gtaatgaagt gatcgcgccg ttttgataat
    47881 ccacccacag tggcttgtta acggcctgat agtcctgaaa atcttcagcg gtaaaggtga
    47941 cgctgtaatc aagaaacatc cgctgcaggc cggtgaatga gtcaaaggta aacagcgttt
    48001 catcggcatc aaagaaaatc cagtcccact tcattattcc accttgtctt acatactgat
    48061 tggcaacgcc atgatgatgg cgtcttcgcg accgtccgtg gtggggtagt aattgcggcg
    48121 aatcgtcgcc tcgttaaagc ctaaactttc gtacagggca atggcggcag catttgaggc
    48181 acggacttcc agccatagtg tcgccacgcc gcgtttttcc agttcgtcga tcagatgttc
    48241 cagcagcacc cttcccaatc cctgacgctg atagtcagga tcgaccgcaa tattgaacaa
    48301 tgtagcttca tccagcacca cttgcgtaat cgcaaacgcc gccattttgc cgttttgcgt
    48361 taactgaaag ttgagataac gctcgccctg gttgctggca aaggtttttt cactccacgg
    48421 aaaggcgtgg gcgcgttgtt caatgtggta agccgctggt aaatcagtcg tttcgaggga
    48481 agaaatcgtg ttcatatgtg caaatctgtt gccataacgc ggcgcgtgcc gttgggtttg
    48541 cccgtaattc ggtgagcgcc ggtgatgcca cctgagcgcc ttccagtgat agcggttcgt
    48601 cagtacccaa ccgccagctg ttgcagcgac tgccttgcgg cagcatcgcg attttttctg
    48661 gcgtcagttg cagcacttga tcggggctga cggttaatgc gcgcagaaca tcgctcacta
    48721 aaggatcggt cagggcggga agatcgtttg ccaccatcac cagacggacg tgtgccggga
    48781 tcgcaatggc aatctccccc tgcaacgcgc cagggcgacg cagcgaccac tgggtaatgc
    48841 ccagttgctg taactgccag tctcgtcggg atgtcatagc gaatcgctcc tgttgtcagg
    48901 ggcgcaaata tagcaaattc gtcgataccg cgccaacata tggctataat cgccgccagt
    48961 atcaattgag gagcattcca tgtctgcatt taccccggca agtgaagtct tgctgcgtca
    49021 cagtgatgat ttcgaacaaa gccgtattct gtttgccgga gacttacagg atgacctgcc
    49081 cgcgcgttta gataccgcgg ccagccgtgc tcatacccag caattccacc actggcaggt
    49141 attaagccgc cagatggggg ataacgcccg tttcagtctg gtcgccacgg tggatgacgt
    49201 cgcagattgc gatatgctga tttactactg gccgaagaac aaaccggaag cccagttcca
    49261 gttgatgaat ttactttctc tgctgccagt ggggacagat atttttgtcg ttggcgagaa
    49321 ccgcagcggc gtgcgcagcg ccgagcagat gctggcagat tatgcgccgt tgaataaagt
    49381 cgacagcgct cgtcgctgtg gcctctattt tggtcgtctg gaaaaacagc cggtatttga
    49441 tgccgataaa ttctggggcg aatacagcgt cgatggcctg acggtcaaaa cgctgcctgg
    49501 cgtgtttagc cgcgacggtc tggatgtcgg tagccagttg ctgctctcga cgttaactcc
    49561 gcacacgaaa ggtaaagtgc tggatgtcgg ctgtggcgcg ggggtgcttt cagttgcctt
    49621 tgcgcgccat tcgccgaaaa ttcgtctcac cttgtgcgat gtctctgcgc cagcggtaga
    49681 agccagccgc gcaacacttg cggccaactg tgttgaaggt gaagtctttg ccagcaacgt
    49741 cttttccgag gtgaaaggtc gttttgatat gatcatctcc aacccgccgt tccacgatgg
    49801 gatgcaaacc agcctggatg cggcgcaaac gctgattcgc ggcgcggtgc gtcatcttaa
    49861 tagcggcggc gagctgcgaa ttgtagcgaa cgccttcctg ccttacccgg acgtgctgga
    49921 tgagacattt ggcttccatg aagtgattgc gcaaaccggg cgtttcaagg tgtatcgcgc
    49981 cattatgacc cgccaggcga agaaaggtta attatctcgc cgaataccgt gtcggatgcg
    50041 gcgtgaacgc cttatccgac ctacaaaacc tgcacgttag cccttcgtag gccagataag
    50101 acgcgccagc gtcgcatctg gcatctgccc ccgcatgcct accaaacctg cacgctagtt
    50161 tcctgatgga catttttcca gcaattacac ctccggcgat aattaactat tgacgaaaag
    50221 ctgaaaacca ctagaatgcg cctccgtggt agcaattctt tttaagaatt gatggtatgc
    50281 gaaggtggcg gaattggtag acgcgctagc ttcaggtgtt agtgtcctta cggacgtggg
    50341 ggttcaagtc ccccccctcg caccaaaaac cacgttgata ttgctcgcac tgggcgaagg
    50401 tggcggaatt ggtagacgcg ctagcttcag gtgttagtgt tcttacggac gtgggggttc
    50461 aagtctcccc cctcgcacca aacgaggcga tatcaaaaaa agtaagatga ctgtgcgaag
    50521 gtggcggaat tggtagacgc gctagcttca ggtgttagtg tccttacgga cgtgggggtt
    50581 caagtccccc ccctcgcacc aattatcttt acttcctttc ttgtttcttc cttgattttt
    50641 catccctgat tcaaattcat tatcatcagc atgatggcag caataagcgc caggcacgat
    50701 ggtagcaaaa cgtaatgccg taactgtttg tgatacagca tcaccggcgt caatactaat
    50761 cccatcagaa tgacaatccg ccactgatcc ggcgataaat ccgcatacat cagccctgca
    50821 atcagcaatc ccggcagcaa cactccccag ccactgccca gcgtacgttg caacatggtt
    50881 tcatctcttt cattgataat gataaccaat atcatatgat aatttttatc atttgcaagc
    50941 cagagaaatc ccttgctatc gggtaaacct atcgctagga ttagcaatca ttatcattta
    51001 gattactatc ccgattatgg cctatcgttc cgcaccgctc tatgaagata tcatctggcg
    51061 aacgcatctc cagccgcagg atgctgggct tgcacaagcc gtacgtgcga cgatcgccaa
    51121 acatcgtgaa catttgctcg agtttatccg cctggatgaa cccgctccgc ttaacgccat
    51181 gacgctggcg caatggtcat caccgaatgc gctaagttct ctactcgcgg tctattccga
    51241 tcatatctat cgcaaccaac caacgatgat ccgcgagaac aaaccgctga tctcactatg
    51301 ggcacaattg tatatcggcc tgatggtgcc accattaatg ctggcgctac tgacgcagga
    51361 aaaggcatta gatgtgtcgc cggaacattt ccatgctgag tttcacgaaa ccggacgcgt
    51421 cgcctgtttc tgggtcgatg tgtgtgaaga taaaaacgca acaccacatt cgccgcagca
    51481 gcgaatggaa acgttaatca gccaggcgct ggttccggtt gtgcaggcac tagaagcgac
    51541 cggagagatc aacggcaaac ttatctggag taataccggt tatttgatca actggtatct
    51601 cactgagatg aagcaactgc tcggcgaggc gacggttgaa tcgctgcgcc atgccctctt
    51661 ttttgagaaa acgctcacga acggtgaaga taatccactc tggcgtaccg tcgtgctgcg
    51721 cgatggcctg ctggtgcgcc gaacctgctg ccagcgttat cgcttaccgg atgtgcagca
    51781 atgtggcgat tgtacgctga aatgaaagca ctgccgggga agtaaacccg gcatcatgcg
    51841 gattaatagg gatgcaacac attacttgct tcgcaatgcc gcatacataa cagaatatct
    51901 gcggcctcca acaaccctgc atcagaactg acgcccagtt tcgacatcac attaaattta
    51961 tgtgcacgga tcgttttgat attgcgttta agctgctcgg caatttgtgt cattgagtag
    52021 ccacgcgaca taaagcgcaa tatttctctc tccgtcgggc ttagcgtccg gctttggtta
    52081 atgtaccact gattgttcag tcggtcggtc gcctgacgta caccatttaa tgacaaaaag
    52141 agttcctgat gaaaaatctc cagcgttgac gctttactta atacaccgtc cagcggtgat
    52201 ggcgacaatg aaccaatcag ccgagcttca atatcatcat ccgcaataac taaacgccgg
    52261 gtacgcggaa acttaatcgc cagttcagtc aggcaagata atccctctct gcgctcactt
    52321 ctcatggcag aaaaagaaaa aatgaccgcc gcaaatgaga tccgcgacat ggcatcctga
    52381 aatgactcct gactggtata ggtatggagc ttataagggt tacccgcaaa ctttctgaat
    52441 aaactctcaa taccgatact gctcatgacg catttttcta tgagtgcgac atttctcttc
    52501 ttaattcggc tgtgttccat tctgcggcat ctccataaac agaaggcaag ttaagttctc
    52561 ttgcaccctg cgcgctattt atccatgcat acatttcagc gttgctgtga attgcc
//
LOCUS       DAETQN010000013        49699 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.013, whole genome shotgun sequence.
ACCESSION   DAETQN010000013 DAETQN010000000
VERSION     DAETQN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.013"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..455
                     /locus_tag="K0W68_000834"
     CDS             <1..455
                     /locus_tag="K0W68_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBI3072483.1"
                     /translation="VPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAY
                     DGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRES
                     LARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
     gene            complement(504..2003)
                     /gene="pitB"
                     /locus_tag="K0W68_000835"
     CDS             complement(504..2003)
                     /gene="pitB"
                     /locus_tag="K0W68_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBI3072484.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(2295..4154)
                     /gene="gss"
                     /locus_tag="K0W68_000836"
     CDS             complement(2295..4154)
                     /gene="gss"
                     /locus_tag="K0W68_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBI3072485.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            4359..5225
                     /gene="yghU"
                     /locus_tag="K0W68_000837"
     CDS             4359..5225
                     /gene="yghU"
                     /locus_tag="K0W68_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBI3072486.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="K0W68_000838"
     CDS             complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="K0W68_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBI3072487.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="K0W68_000839"
     CDS             complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="K0W68_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBI3072488.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="K0W68_000840"
     CDS             complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="K0W68_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBI3072489.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(6424..6918)
                     /locus_tag="K0W68_000841"
     CDS             complement(6424..6918)
                     /locus_tag="K0W68_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBI3072490.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="K0W68_000842"
     CDS             complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="K0W68_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBI3072491.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="K0W68_000843"
     CDS             complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="K0W68_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBI3072492.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="K0W68_000844"
     CDS             complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="K0W68_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBI3072493.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="K0W68_000845"
     CDS             complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="K0W68_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBI3072494.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(12082..12369)
                     /locus_tag="K0W68_000846"
     CDS             complement(12082..12369)
                     /locus_tag="K0W68_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBI3072495.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(12488..13375)
                     /locus_tag="K0W68_000847"
     CDS             complement(12488..13375)
                     /locus_tag="K0W68_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBI3072496.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            13532..14572
                     /gene="gpr"
                     /locus_tag="K0W68_000848"
     CDS             13532..14572
                     /gene="gpr"
                     /locus_tag="K0W68_000848"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBI3072497.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(14612..15106)
                     /locus_tag="K0W68_000849"
     CDS             complement(14612..15106)
                     /locus_tag="K0W68_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBI3072498.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            15297..16181
                     /gene="yghA"
                     /locus_tag="K0W68_000850"
     CDS             15297..16181
                     /gene="yghA"
                     /locus_tag="K0W68_000850"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBI3072499.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="K0W68_000851"
     CDS             complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="K0W68_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBI3072500.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="K0W68_000852"
     CDS             complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="K0W68_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBI3072501.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            17759..18946
                     /gene="metC"
                     /locus_tag="K0W68_000853"
     CDS             17759..18946
                     /gene="metC"
                     /locus_tag="K0W68_000853"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBI3072502.1"
                     /translation="MTDKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            19086..19745
                     /locus_tag="K0W68_000854"
     CDS             19086..19745
                     /locus_tag="K0W68_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3072503.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="K0W68_000855"
     CDS             complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="K0W68_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBI3072504.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            20878..22041
                     /gene="yqhD"
                     /locus_tag="K0W68_000856"
     CDS             20878..22041
                     /gene="yqhD"
                     /locus_tag="K0W68_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBI3072505.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            22146..22973
                     /gene="dkgA"
                     /locus_tag="K0W68_000857"
     CDS             22146..22973
                     /gene="dkgA"
                     /locus_tag="K0W68_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBI3072506.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            23173..24099
                     /locus_tag="K0W68_000858"
     CDS             23173..24099
                     /locus_tag="K0W68_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBI3072507.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            24150..24407
                     /gene="yqhH"
                     /locus_tag="K0W68_000859"
     CDS             24150..24407
                     /gene="yqhH"
                     /locus_tag="K0W68_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBI3072508.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24450..26669)
                     /locus_tag="K0W68_000860"
     CDS             complement(24450..26669)
                     /locus_tag="K0W68_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBI3072509.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(26780..28192)
                     /gene="ftsP"
                     /locus_tag="K0W68_000861"
     CDS             complement(26780..28192)
                     /gene="ftsP"
                     /locus_tag="K0W68_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBI3072510.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(28267..29004)
                     /gene="plsC"
                     /locus_tag="K0W68_000862"
                     /gene_synonym="parF"
     CDS             complement(28267..29004)
                     /gene="plsC"
                     /locus_tag="K0W68_000862"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBI3072511.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(29237..31495)
                     /gene="parC"
                     /locus_tag="K0W68_000863"
     CDS             complement(29237..31495)
                     /gene="parC"
                     /locus_tag="K0W68_000863"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBI3072512.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(32041..32523)
                     /locus_tag="K0W68_000864"
     CDS             complement(32041..32523)
                     /locus_tag="K0W68_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3072513.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(32576..32968)
                     /gene="ygiW"
                     /locus_tag="K0W68_000865"
     CDS             complement(32576..32968)
                     /gene="ygiW"
                     /locus_tag="K0W68_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBI3072514.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33120..33779
                     /gene="qseB"
                     /locus_tag="K0W68_000866"
     CDS             33120..33779
                     /gene="qseB"
                     /locus_tag="K0W68_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBI3072515.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33776..35125
                     /gene="qseC"
                     /locus_tag="K0W68_000867"
     CDS             33776..35125
                     /gene="qseC"
                     /locus_tag="K0W68_000867"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBI3072516.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            35235..35816
                     /gene="mdaB"
                     /locus_tag="K0W68_000868"
     CDS             35235..35816
                     /gene="mdaB"
                     /locus_tag="K0W68_000868"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBI3072517.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35847..36161
                     /locus_tag="K0W68_000869"
     CDS             35847..36161
                     /locus_tag="K0W68_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBI3072518.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(36209..38101)
                     /gene="parE"
                     /locus_tag="K0W68_000870"
     CDS             complement(36209..38101)
                     /gene="parE"
                     /locus_tag="K0W68_000870"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBI3072519.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(38130..38711)
                     /gene="yqiA"
                     /locus_tag="K0W68_000871"
     CDS             complement(38130..38711)
                     /gene="yqiA"
                     /locus_tag="K0W68_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBI3072520.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(38711..39538)
                     /gene="cpdA"
                     /locus_tag="K0W68_000872"
     CDS             complement(38711..39538)
                     /gene="cpdA"
                     /locus_tag="K0W68_000872"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBI3072521.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(39563..39985)
                     /locus_tag="K0W68_000873"
     CDS             complement(39563..39985)
                     /locus_tag="K0W68_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBI3072522.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(39986..40615)
                     /gene="nudF"
                     /locus_tag="K0W68_000874"
     CDS             complement(39986..40615)
                     /gene="nudF"
                     /locus_tag="K0W68_000874"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBI3072523.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            40820..42301
                     /gene="tolC"
                     /locus_tag="K0W68_000875"
     CDS             40820..42301
                     /gene="tolC"
                     /locus_tag="K0W68_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBI3072524.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            42449..43120
                     /locus_tag="K0W68_000876"
     CDS             42449..43120
                     /locus_tag="K0W68_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBI3072525.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            43126..44286
                     /locus_tag="K0W68_000877"
     CDS             43126..44286
                     /locus_tag="K0W68_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBI3072526.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(44324..45112)
                     /gene="ygiD"
                     /locus_tag="K0W68_000878"
     CDS             complement(44324..45112)
                     /gene="ygiD"
                     /locus_tag="K0W68_000878"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBI3072527.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            45255..46028
                     /gene="zupT"
                     /locus_tag="K0W68_000879"
     CDS             45255..46028
                     /gene="zupT"
                     /locus_tag="K0W68_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBI3072528.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(46086..46256)
                     /locus_tag="K0W68_000880"
     CDS             complement(46086..46256)
                     /locus_tag="K0W68_000880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072529.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(46517..47170)
                     /gene="ribB"
                     /locus_tag="K0W68_000881"
     CDS             complement(46517..47170)
                     /gene="ribB"
                     /locus_tag="K0W68_000881"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBI3072530.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(47274..47422)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            47544..47834
                     /gene="ubiK"
                     /locus_tag="K0W68_000882"
     CDS             47544..47834
                     /gene="ubiK"
                     /locus_tag="K0W68_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBI3072531.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            48117..48668
                     /locus_tag="K0W68_000883"
     CDS             48117..48668
                     /locus_tag="K0W68_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HBI3072532.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            48728..>49699
                     /locus_tag="K0W68_000884"
     CDS             48728..>49699
                     /locus_tag="K0W68_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI3072533.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQ"
BASE COUNT        12448 a        13185 c        12329 g        11737 t
ORIGIN      
        1 cagttccggc gctggtaatt acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata
       61 tgcttgcctg tcgtcgtcgc gggttaattg ttctaaccga ccgttatcct caggaccaaa
      121 ttcctggcgc ttacgatggt acggtgttcc cacctaacgt tgaaggtggt cgttttgtct
      181 catggctggc aagccaggaa cgtaaagcgt ttcactggat ggcgagccat aagcctgatc
      241 tggtcatcaa actcaatgtt gaccttgaag ttgcctgtgc acgtaaaccc gaccataaac
      301 gggaatcgct ggcgaggaag attgccataa cgccacagtt aacctttggt ggtgcacaac
      361 tggttgatat cgatgccaat cagccactgg aacaggtgtt ggttgatgca gaaaaagcga
      421 ttacggattt tatgaccgcg cgtggttatc actagtcaaa aatggaaatg cccgatcgcc
      481 aggaccgggc attttcagga aggttaaatc aactgcaatg ctatccagta cagcccacca
      541 gaaagaaaaa ttgccgccgg taaagtaaac acccacgcca tcaggatgct ggttaccgtt
      601 ttacgctgta acccaccgcc gtccaccacc atcgtccctg ctaccgcaga cgagaggacg
      661 tgtgttgtgg agacaggcat cccaatataa ctggcaaggc cgatagacac tgccgccgtc
      721 atttgtgccg ccatgccttg cgcatacgtc atgccgcgct taccaatctt ctcaccgatg
      781 gtcatcgcta cacgacgcca gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc
      841 atgatgatcc acaccggagc gtactcaatg gtgcttaaca tatcgctgcg cagttttttc
      901 agcaggttct ggtcttcttt actgacgcct ggcagtttcg ccagcttcgc ggaggtatca
      961 gagatgcaca gcataatgcg gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa
     1021 ctttccatat tgcctggcag catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc
     1081 ggatgacagt gaaactctgt tacttgcgtg ccatcagtcg atgctgcagg caatggaggt
     1141 tccatcgcaa tcaacttctg cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg
     1201 gtgacggcat cgcgggtacg ggtaatttca tagccggacg cgttcatatt gacgacgaag
     1261 ccagcagggg caatccccac cagtaccagc atcaccaggc cgatcccttt ttgtccgtcg
     1321 ttcgcgccgt gcgaaaacgc cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag
     1381 aatggcggtt tacgtttgcc ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg
     1441 tcacgctttt tcgtcccgct ccagtagcgt cgcagcagga atatcaggcc tcccgcaatg
     1501 accaggccga cgatagggga aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg
     1561 tttaacgcat ccatcaccga tgagccggtt aacagcgcgt tggttaaacc gatgccgata
     1621 atcgcaccaa tcaaggtgtg cgaactggag gccggtaaac cgaagaacca cgttcccagg
     1681 ttccagataa tcgccgccag cagcatggaa aagaccatcg ccaggccgtg ggttgacccc
     1741 atattcagca acaaatcggt tggcaacata tggacaatgg cataggcaac gctaagtccg
     1801 cccaataaca cgccaaaaaa gttaaaaaat gccgccatca ccacagcaag ttgtggttgc
     1861 atggcacgag tataaataac ggttgccacc gcattcgccg tgtcatgaaa accattgatt
     1921 gcttcgtaga acaacacaaa tgccagagca agcaataaca aaagccctgt gtatatatca
     1981 aggccaacaa ataaatttag catatattag attacgccat tttgaattta cgaacggacg
     2041 cattatcagt gactttaacg gcatgggcaa agtgaaatat cattttttaa cgataaaaag
     2101 agtgttattt cttgtgtatc agacaataag cattcattca aaacctttag aaataatcaa
     2161 aacacaggta ttccactggt taatatattc acatatgaaa taaataaata ttagaacata
     2221 taaatattga atattttgat taatccccgt actgattatt cttcataatc agtacggggt
     2281 tgctacaaca tgtattactt tttcaccaca attaacggtt caatatcact ctcttttttg
     2341 atgaccagtg attcatcacc gcgcaaacac gtcccaccgt agttgccgcc cacggtgaag
     2401 gtacatacct gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg
     2461 tttttctgct cggcaaattt accgctggtt ttgtccagca cctcttcatg atggctgacc
     2521 aggtcgatat tgctaccaca gcgaccggca atcggtttca ctgcgtaacc tgttttcacc
     2581 aattcatcat taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac
     2641 cagaggatcg gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagact
     2701 agcacttccg ggcgcagcaa tacgtcgata agacgcactt cgttttgcgg atgaccggta
     2761 cggattggca ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt
     2821 tcccacgccc aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc
     2881 ccggcagcat cccagcccag ttcatccaat ccacgcaaga tacgcgtttc aaagcccgcc
     2941 tggtgcagcg cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc
     3001 atgatatgga caaacggacg tgcacgactg tgtttccagg caccagccaa ttcgttaatc
     3061 agcccttccg ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg
     3121 atcaagcccg cttcggtatg acaggaggcg gagtcggcgt tgtactcgta aaccttcagg
     3181 ccacgctcat ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc
     3241 caggagagac gcaaacgtgg ccagaggatt ttcgggatgt cgaacagcgc cagcaggttg
     3301 tcatctttca gcaccttgtc ggttgcgtga agatacatca ggtgcagctc gttggtggct
     3361 ttaattagct cctgctcggc actctcggta atggtgtagt aatgataagg atcctgattg
     3421 atcacctgac cgttggcctg cacataggcg ttttgcagcg gatctttttc atccagccat
     3481 ttaccgtcaa actggccttt gttttccagg cgcgctccgc tgattttcag cagctcgcct
     3541 gcaatttccg gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga
     3601 atggtggtgt catcaaaagt gtctttcagg gtatagcagc cgttttcgac caccatctcc
     3661 agctcacgcg tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca
     3721 atacggactt tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat
     3781 tcaccgcctt tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta
     3841 ggaaatgcct gcaatggcag gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag
     3901 aaaatctccc acgccatacc cacgtcagta aagaccacac cgtaattcag aaagagaaaa
     3961 cggcgagcaa attcaacgca ttgccacttg tggcccatat attcgtcgtc gatatagcta
     4021 cggaatacgg cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag
     4081 attgctaccc cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc
     4141 gttcctttgc tcatcacttt acctttaaca atacaaccta agcagttggc gtaattgttg
     4201 tgctctgatt acctgctcag cgacattaac cggacagagg tcagactaat catacacctc
     4261 atcgcagcgg ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct
     4321 acactgcttc aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc
     4381 ccgcgaaagt ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc
     4441 cggtttctgg tccgacgcat gaaaaaacgc tgcccgttgg caaacaccca ttgcaacttt
     4501 attcgctggg aacgccgaac ggtcagaaag taacgattat gcttgaggag ctgctggcgc
     4561 tgggcgttac tggtgcagag tacgacgcct ggctgattcg tattggcgat ggcgatcaat
     4621 tctccagcgg ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata
     4681 cgcataatcc gccgatccgc gtgtttgaat ctggttcgat cctgctttat ctggcggaga
     4741 aatttggcta cttcctgccg caggatttgg caaagcgtac tgaaacgatg aactggctgt
     4801 tctggttaca gggcgcggca ccgttcctcg gcggtggttt tggtcacttt taccattacg
     4861 cgccgatgaa aattgagtac gccatcaacc gctttaccat ggaagccaaa cgtctgctcg
     4921 acgtgctgga taagcaactg gcgcagcata agtttgttgc gggcgatgag tacaccattg
     4981 cggatatggc gatttggccg tggtttggca atgtggtgtt aggcggcgtg tatgatgccg
     5041 cagagtttct tgatgcgggc agttataagc atgtacaacg ctgggcgaaa gaagtaggcg
     5101 aacgtccggc ggtgaaacgt gggcgtattg ttaaccgcac caacggaccg ctgaatgagc
     5161 agttgcatga gcgccatgac gccagtgatt tcgagacgaa tacggaagat aagcgtcagg
     5221 ggtaagggtt ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca
     5281 tccggcaatc gtgcacaatg cctgatgcga cgctgccgcg tcttatcagg cctacgaaag
     5341 ctaatcatca cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt
     5401 tggcttcatc ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc acccactggc
     5461 ccagtagatc ggcagggtta ccttcacaaa tcagggcgat attcacgtcg cgcttgatac
     5521 cacatacttc aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggcctg
     5581 gaacgccaat acacattatt aactccggtt attcaacttc aatacttttg acgatcagcg
     5641 aatcgccggt atcgacacgc aaccgctcgc cgtgacagag cggacactgc gcatcgtgct
     5701 gatgaatctc caccacctgg ctgcaatccc agcaccaagc ctgggcgggt ttatagacga
     5761 tatgtaaatc gcacccttgc gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga
     5821 cggcgctctc ctcaacgcag gagagcgcgc caatttccag ccacacggcg gtgacgcgct
     5881 taacatcgtg ctgctccgcc tgccgttgga taatttcaac ggcgctctgg caaagagaca
     5941 actcatgcat tttcgccact cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt
     6001 acatccggat tcgtgactgg cagcgaaagg atcattcgtg cgcagtcatc cgtcaggcgt
     6061 tggccctctt caatcgacat gctgtgcgaa agcggcgaca tcagcgaaca ggagagatat
     6121 tgcgaaacac cgtccagttc gccaacggta aaggtcatag tgccatacgg cagttgcaga
     6181 ccaatttttt cactcacttt gcgcagcggc cagagttgat ccgggccggg gaagataact
     6241 gcactcagca tccacggggt gatcacacac cccgtccact gaccttcgaa cagcgtaaaa
     6301 tcagaaacat acaccggcat tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg
     6361 gcaatttctt caaacgctgc ctgtacttgc gccttcgggg aggtctggaa acctgctatc
     6421 tcttcagtca tgaatcgcct cccgtgggat ggcttccacg cctgattcgc gcagcgcagc
     6481 cagaacctgc tcaagcgcag gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg
     6541 tggctccagc gattccggga tcacgccgac cagggtcagc tttttcggaa actcaccggt
     6601 gaagcgcagg gccgacaata cgtcggccag gccaagctga tgcggagaga ttttgttggt
     6661 aaacaacgcc ggaacttctt catcccgcag gatcatcatc gttcccggcg tgttcttttt
     6721 cgacacaatg gcatccgcaa taatcagatg atcgcgattt gccatgtcgc caagcagctc
     6781 cattcccgcc gtgccgccat cgaggatctc aacataatcc ggcagaatgt atcgttgctc
     6841 taacgcttcg acaatccgca caccgatggc ttcatcggtc agcaaaatat tgccgacccc
     6901 taagactaaa atacgcatta cagaaccttc actgaaacca cttcgttgcc gtcggcatcc
     6961 actacgtgta ccgcacaggc catgcacggg tcgaaggagt gaatggtacg caccacttcc
     7021 agcggtttat tcggatcagc aaccggtgta cccaccagcg actgctcgta aggaccgacg
     7081 tcatcattga agttacgcgg accagagttc caggttgatg gaacaactgc ctggtagtta
     7141 ctgataatac cgtctttaat aaccatccag tgagagagca taccgcgcgg cgcttcgagg
     7201 aagccaacac ctttgaactc acccgttgcc ggaatgttcg gtttcacaaa ggtggtgtga
     7261 tcgcctttgc cgatattggt gatcagtgca ctgtattgat tttgcaggat atcctgcaat
     7321 tcgcagcagt gaacggtacg accaataata cggcccagcg tggagtgcag ctgcgccact
     7381 tccagcgtgt tgccagtcag tttctgataa atcgcaacga tttcattcag tttgttctgg
     7441 gtagattcgc gacctgccgc cagtttcacc agcatattag ccagcggacc cacttctacc
     7501 gttttgccgt agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc agaccaacca
     7561 tcataagccg gaatggtggt accttcccac ggtgcctgcg gcgcttcgtc tttataccag
     7621 gagtgcttcg cgctttcctg aatgcctttg atcaggtatt catcggaatg agaagtgatc
     7681 ggacgatacg aggacagatc cgcattctca atgtagccgc ccgggaacag gaagctgccg
     7741 tttttactgt cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc
     7801 gtcagccatt ccgggtagaa cgcggcaata actgcggtat caaccttata aacctgttca
     7861 acaaagtcgc tcagcttgtc gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc
     7921 acgcccaaac cgtcgaggtt gatcgggttc gcgacaccac ctaccgccag gttctgaatg
     7981 tgcggcgttt taccgcccag cagcgccacg acgcggttag cgtcacgctg gcactccagc
     8041 gcctgcaggt agtgcgctac cgcaatcagg ttcacttccg gcggcagttt catcgccggg
     8101 tgaccccagt agccattggc gaaaataccc aactgaccgc tggcaaccag atctttgatc
     8161 ttgttctgaa ctttagtgaa ctcttccgga ctgttcagat gccaggtcga aacgcctttc
     8221 agcatttcgg aggctttggt tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc
     8281 agcgccgaaa gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc agccagaatg
     8341 atgttacgga tgtattgcgc gttaaccgga acgtcgatat tcagcgcact ttctgccgca
     8401 cgaacggaag acagcgcgtg agtggtagta catacgccac agatacgttg cacaatcatc
     8461 catgcatcgc gcggatcgcg gtttttcacg atctcttcca tgccgcgcca catggtaccg
     8521 gaagcccatg ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg taaatgcccc
     8581 tcaatacggg ttaccggatc aatagtaatt ctctggctca tgctttgctc gcctcatgac
     8641 gattatgatc gttttgtttt aaaggaggaa gtatcggcag tagacgaatg agtacgatgt
     8701 aagcgcaaat ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc caggtcggga
     8761 agtaggcgta accgccgccc gggttgaatg ccaccagcga ataggtcaga cgccaggttg
     8821 cacaacctaa cagtgcgctc agtgctgaca ggaacagcat gcgggaatca ttacgcagct
     8881 tcgccacacg cagaacgacc agcgggaaga gcatcagcag gacttcaatc cagaacatca
     8941 cggagtagaa gtcaccggca aacgctaacg acagcttgtc gcgatagata agctcgccaa
     9001 agcgcagcac gatgaaaatc gccagcaaca cactgatggt gttggtcagc ttgacgaaca
     9061 gactcttttc atccggaccg ttgccacgca gacccgcctg caccagcgaa ccttcaaaga
     9121 tgacaatcga gaagcccatg atgaacgccg tcagcagcga gaacagcggc aacatttcat
     9181 agctctgcca caacggatgc accttgtagc ccgccgagat catcagtgac cccattgaag
     9241 actggtgcat ggtcggcaac agcgcgccaa gtgcaatgat gaagaacata accttgttca
     9301 gacgcttgag agaaaccttc cagcccagac gttcaaacag tgccggagca aactccagtg
     9361 ccatcacgcc gatatagatg gtcatacaga ccgccgtctc gaacagtacc gagttcacgt
     9421 tgaagtgacc cggaatgtag aagtacggca ggttccagta gcgacccacg tcgatagtga
     9481 tcgacaagcc acccagtgag taaccaaaca gactcgccaa cagcgccgga cgcaccagcg
     9541 gatggtattg cccacggtta aagacgtata ccgcccacgc cagcgcccag ccgccacagg
     9601 caaagccggt gccaatcagc aggtcaaacg cgatccacac gccccacggg aagccgccgt
     9661 tcaggtcaga gacagagccc agaccgaaca ccagacgctt cacaatcagg agcatacaga
     9721 tgacgattaa cggtccaaaa atcatgaccg gtttactgat gattttgccg cccagcggtt
     9781 gtggatcatg actcatgatc gtctcctccg tcgtgatggt cgtttttggt gttgcgacga
     9841 accagcacgg ttaagcccgc cagcacagcc agtggtagca tcatgccttt atacagggtg
     9901 tgttgaatat tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga
     9961 ttttcataag gcacacccgt cagtaccaga acctgagtac cgccgccctc tttctcgccg
    10021 tacagatgcg gataatattt cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc
    10081 tgacgcggat agtggtattc gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc
    10141 atcagctctt cacgcgtacc gaaaatcacc gcgcccgccg ggcacacttc tacgcagcca
    10201 ggcagaccgc ctttatcgag acgttccaca cctttctggt tgcacagctc gcacttatgc
    10261 agcgcaccaa acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg
    10321 cagtaacggc aaccggtgca cacgtctttg tcgtaatgga caatgccggt tttcggatct
    10381 tttttcagtg cagagaccgg gcacacagag acacagttcg gatcgacgca gtgcatacac
    10441 tgtttcttaa tgtacgcgta gccgttctcc tcctggtctt tgttgacccc tgtgccgctg
    10501 gtccacacct gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc
    10561 tgttcccctt gcgggttacg ttcagggaag ttgatatcct gacacttggt gacgcaagcc
    10621 tggcagccta cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg aattggcggg
    10681 cggttttcag cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag
    10741 gaggctgctt taataaaatt acgtctgttc acggttattc tccccgtgag tcagcgttat
    10801 ctttcttttg ctgacgaccc agttcacgca ccgccatcac gctgacaccg gcaaccagcc
    10861 caaccacacc gccgagcaaa ccaatagcgc ctgcagagac gttgccgccc tctttagcgt
    10921 taacatccgg tttctgtgaa cgcggagtct gattttcgac gttggcaagc tgatggatgc
    10981 ctttatggaa gccgatacct tcttcgttac agccatagca tgggtgacca atcgctaccg
    11041 gccacacgcc gccaacatcg cagaattgca gcgttgagca gttgccgtaa gtttctggcc
    11101 ctttacagcc gaggtggtac aggcaccagc cttcacggtg gccttcatca ccgaactctt
    11161 tggcaaaacg accagcatcg aagtgcgggc gacgttcgca gtgttcgtga atcagacggc
    11221 cataggcgaa ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg taagtgatga
    11281 tgtgcgcaac ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga atattgataa
    11341 cggttttgcc tggcagaact tcttgcaggc tgactgcgcc agttgggtta actccagctg
    11401 cggcaacacc gccccacgca gagcaggaac cgatagcgat aatcgctgct gcgccttctg
    11461 ccgctttgcg gatgtgatcc acaatcggct caccagcaac catgcaataa ataccgttat
    11521 cttttaatgg gatggaacca tccacaacca acacatactg ccctttgtac ttctcaagtg
    11581 cgttatgttt gttctcttcg acctgatgac cgaaggcggc ggaaagcact tcgtgatact
    11641 ccagagagat agtctccagc acgaggtttt ctaccgttgg atgcgttgca cgaagcagag
    11701 attccgtaca accggtgcac tcctgcgcgc caatccagat aactggcgga cgctgcgggt
    11761 tagtaaccga ttcggccatc tctgcagcgg ctttgctact taaccccatg gtggcggcta
    11821 atgctgcaca aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt
    11881 tatctccagt catttatagt tattccgttg cgaagacctg gcatatattt tgcctcaatc
    11941 gcaaaatcaa taatgcgatc gatgcgccat ttaccacaca tttattatgg ttatcgatat
    12001 catgatactg cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag
    12061 caagataata cgtatgtttg atcaattttc gaggcgataa ccggccataa aaaagcgcaa
    12121 cgtagaacag gaattattct cacgaaagaa atccattacc atctctttat ccagtccata
    12181 gcggcgcgtc agaatacccg cttcccaggc gctaagctgg cgatctccgg tcttttcgta
    12241 catccggtgt gagtagccta atacaaaacc gcgtttataa tcggcacaga attttgtcac
    12301 attaaccgca ctgtcggcat gcgttgcttt taatcccgcc attaagcctt taccaaaatg
    12361 gttattcatc gttcacctca ttcgctatat atcgccatat atagcgatat tttaggcaaa
    12421 gaacgacttt tgcgcacgct attttttcat gcttgtttaa gacgtaatcc agtaagagat
    12481 aaacctatca ggagagatat ttatcgaacc atttcagtgt cctttgccag gcaagatcgg
    12541 cggcagattt gtcataacgg ggcgtggaat cattatggaa tccgtgatta acccccggat
    12601 agatatacgc ctcataaacc ttattattgg ctttcaacgc cgcctcgtaa gcaggccagc
    12661 cctcgttgat tcgggtgtcc agttccgcga agtggagtag taaaggcgct tcaatcttcg
    12721 ccacatcggc agtgggtgcc tgacgaccat aaaacggcac cgcgcaggcc agttccggat
    12781 acgcgacagc cgccgcgttc gatacgccac cgccatagca aaatccggta atacccactt
    12841 tgcctgccgc ttgtggatag cgttgcataa actcaattgc ggcaaagaaa tcattcatca
    12901 gtttggttgg atcaacctgc tgttgcagct cacgaccttt atcatcattt cccggataac
    12961 ctccaacgga acttaagccg tcaggtgcca gggcgatata ccccgccttc gccactcgcc
    13021 gtgccacatc ttcgatatac ggattcagtc cacgattctc atgcaccacc accacggctg
    13081 gcgttttgcc gctcattttt gcgggtttca ccagataacc ccgtacctcg ccgtgaccat
    13141 ttggcgaagg atacatgatg tactcagcaa caatctccgg gtcggtaaac tctacctgag
    13201 tcgccagcgc ataatttggc ttgagcaaat caaacaacgc taatgccgtc atcccgccca
    13261 ccgcatactt cgccgcaagg ttgaggaact cacgtttcga aattttcccg tgagcgtaat
    13321 agtcgtagta atccaacaac tcttgtggga aatctttggc ggttaaacgc ggcatcgttg
    13381 cactcctcag ttggtgtttt tttaagcaaa gcataagcac gtgtttttgc ccagtttttc
    13441 gtcactctgt gagccagact acgggatacg cgctggcgaa tcgctaaact agaaacattg
    13501 tttcgaaatt gaacggtgga aaggagaggt catggtctgg ttagcgaatc ccgaacgtta
    13561 cgggcagatg caataccgct attgcggaaa aagcggttta cgcctgcccg cgttatcgct
    13621 cggtttatgg cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg cgatcctgcg
    13681 taaagcgttt gatttgggca ttacgcactt tgatttagcc aacaattacg ggccgcctcc
    13741 aggaagcgca gaagagaact ttggtcgcct gctgcgggag gattttgccg cttatcgcga
    13801 tgaactgatt atctctacca aggctggcta cgatatgtgg cccggccctt acggctctgg
    13861 cggttcacgt aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta tagggcttga
    13921 gtatgtcgat atcttttact ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc
    13981 ctctgcgctg gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga tctcctctta
    14041 ctcgccagag cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa ttccgctttt
    14101 aattcatcaa ccttcgtaca atttactgaa ccgctgggtg gataaaagcg gcctgctgga
    14161 taccctgcaa aataacggcg tgggctgtat tgcctttact cctctggctc agggattgct
    14221 gaccggaaaa tatctcaacg gcattccgca agattcacgg atgcatcgtg aagggaataa
    14281 agttcgtggt ctgacgccga aaatgcttac cgaagccaac ctcaacagcc tgcgcttatt
    14341 gaatgaaatg gcacagcagc gtggacaatc aatggcgcaa atggcgttaa gctggttgct
    14401 gaaagatgat cgcgtgacgt cggtattgat tggtgccagc cgcgcggagc aactagagga
    14461 gaacgtgcag gcgctgaata atctgacatt tagcaccgag gagctggcgc agattgatca
    14521 gcatatcgcc gatggcgagc tgaatctgtg gcaggcgtcg tccgataaat gacctgttaa
    14581 taacgggcgc gcgaaccgcg cccgaatcag atcagtgatt atgacgagtc agtcggtcaa
    14641 gatagcccat cacaaatgca gaaagcacaa acgtcagatg gataatgacg taccacatca
    14701 gtttgttatc cgggacattt ttcgcatcca taaagacgcg cagtaagtga atggaagaaa
    14761 ttgccacaat cgacgctgct actttgtttt tcagcgacgt tgcgtccatt ttccccagcc
    14821 agttcagctt ctctttgttc tcggagatat ccagctgtga gacgaaattc tcataaccgg
    14881 aaaacatcac catcaccagc aaaccgccaa ccagcgtcat atccaccagc gacagcaaca
    14941 cgaggatcaa atctgattcc gccatcgaga agatattcgg cagtacgtga ataatctcct
    15001 ggaagaactt cagcgccagg gcaactaacg ccagcgaaag gccaaagtac acgggggcaa
    15061 gcagccagcg agaagcatac attgcatttt caagaaaacg ttccatacat tcctgtcgtg
    15121 tagttaaaac gggcaacagt atatcgcaat tgagtaaaca gcacgcaaca gcaaatcaac
    15181 atatacgtta aacctttgtc gcataattct gaggtttagg gatcttcctg gttcccttat
    15241 caacgcatcg agagcgtcta cccttaaagg acgttgaacc tgaagggaga aaaacgatgt
    15301 ctcatttaaa agacccgacc acgcagtatt acactggtga atatcccaaa cagaaacaac
    15361 cgacgccagg catccaggcg aagatgacac cggtaccgga ttgcggcgag aaaacctatg
    15421 ttggtagcgg tcgcctgaaa gatcgtaaag cactggtgac agggggcgat tccggaatag
    15481 gtcgcgctgc cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc agttatcttc
    15541 ccgtggaaga agaagacgct caggatgtga aaaagatcat tgaagaatgc ggacgcaaag
    15601 ccgttctgct gccaggcgat ttaagcgatg agaaattcgc ccgttcgctg gttcacgaag
    15661 cgcacaaggc gttaggcggg ctggatatta tggcgctggt cgccgggaaa caggttgcca
    15721 ttccggatat tgcagacctc accagcgaac agtttcaaaa gacctttgcc attaacgttt
    15781 tcgcgctgtt ctggctaacc caggaagcga tccccctgct accgaaaggt gcaagtatca
    15841 tcaccacttc gtcaatccag gcataccagc caagtccgca tttactggac tatgcggcta
    15901 cgaaggcggc gattctgaac tacagccgtg gcctggcaaa acaggtcgcg gagaaaggta
    15961 ttcgggtgaa tattgtcgcg ccaggcccta tctggacggc actgcaaatt tccggcggac
    16021 aaacgcagga taagatcccg cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac
    16081 cggcggaact ggcccctgta tatgtttatc tggcaagtca ggagtcgagc tacgtcaccg
    16141 cagaagtgca cggcgtgtgc ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa
    16201 aagcaaccgg gcatcattgt gaattcactc ttacctgatg tggcgaaaac gccttatccg
    16261 gcctacaaac gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta
    16321 gcgcatcagg caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc
    16381 ttcaggtaac ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg
    16441 gttttgtccg ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac
    16501 gccgtaatca ttgtttcatc ggtgaccgga tcgttaccga taaacatcga gttgtctgcc
    16561 ttcaccgaca gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc
    16621 aagttcacct tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga
    16681 accaacatca cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg
    16741 aggttttcgt taagatgcat tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca
    16801 cgaaccggat gcgcagcggc gctggcttcc agatccaggt cacggctttg cagcaacaat
    16861 acctgcgctg caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg
    16921 ttatagataa cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct
    16981 tctgcgatac ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata
    17041 aagctgttca tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg
    17101 atggttgcca gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc
    17161 tccagacgga agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt
    17221 tccagctcat tctgcgcttc attgagcaaa tgcaggctta agcttttgct accaaaatca
    17281 gcggcgatat cgttggcctg gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc
    17341 ttaaggcgac gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaagtg
    17401 actacggagg ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtgctga
    17461 tacatacccc agacggaaag gtccgtctgc atcaaattat tacccactgt gtatctccag
    17521 gacgcaagtc acaaaatctg cgcataataa tatcaaaatg acgtcgaatt gatagtcgtt
    17581 ctcattacta tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa
    17641 aaaagtcacc agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc
    17701 gcccgcgaat attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgagat
    17761 gacggacaaa aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct
    17821 cggcgcggta aatagcgtga ttcagcgcgc ttcttcgctg gtctttgact gtgtggaagc
    17881 caaaaagcac gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac
    17941 gttaacccat ttctccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgt
    18001 gctatttccc tgcggggcgg cagcggttgc taattccatt cttgcttttg tcgaacaggg
    18061 cgatcatgtg ttgatgacca acaccgccta tgaaccgagt caggatttct gtagcaaaat
    18121 cctcagcaaa ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt
    18181 taagcatctg cagccaaaca ctaaaatcgt gtttctggaa tcgccaggct ccatcaccat
    18241 ggaagtccac gacgttccgg cgattgttgc cgccgtacgc agtgtggtgc cggatgccat
    18301 cattatgatc gacaacacct gggcagccgg tgtgctgttt aaggcgctgg actttggcat
    18361 cgatgtttct attcaagccg ccaccaaata tctggttggg cattcagatg cgatgattgg
    18421 cactgccgta tgcaatgccc gttgctggga gcagctacgg gaaaacgcct atctgatggg
    18481 ccagatggtc gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt
    18541 gcgtttgcgt caacatcatg aaagcagtct gaaagtggct gaatggctgg ctgaacatcc
    18601 gcaagtagcg cgagttaacc accctgctct gcctggcagt aaaggccacg aattctggaa
    18661 acgagacttt acaggcagca gcgggctatt ttcctttgtg cttaagaaaa aactcagtaa
    18721 tgaagagctg gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg
    18781 cgggtatgaa tcgttgatcc tggcgaatca accagaacat atcgccgcca ttcgcccaca
    18841 aggcgagatc gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga
    18901 cgatctgatt gccgatctgg acgccggttt tgcgcgaatt gtataacatt gccacttttg
    18961 gacaattttg cagacattta attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc
    19021 gtcccggacg aatcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa
    19081 agtccatggc tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc
    19141 tggcggatcc tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag
    19201 aaaacggcct gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg
    19261 cattgattgc ccaaggggtt atggattttc tgcctacgat tgcgattctg accgccgcag
    19321 caagtctggg ctgctggcta agttatattc aggggcgctg gttagggaat accaaaacgg
    19381 tgaaaggctg gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg
    19441 accgccacgg tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc
    19501 tgccaaccat ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt
    19561 taagtggatt gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc
    19621 cgttcgttaa acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct
    19681 tgttaaccgc tggcttgtta ggcacgctgt ttgtagtgat taaaaaaaaa tactgtaacg
    19741 cctgacgatt ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc
    19801 gcccgcattc ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg
    19861 ctaaattgcg atgcgctttc atagtcgacg cgcatcgctg ctgcgctggc cttcatgccg
    19921 tcatggatga tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc
    19981 gaggtgctgg tgacagactt aaaattatga tggaacgccg atacgctcat gttggcttct
    20041 gccgccagtt gctcgacgct caggttttcg gtgtatttat tctcaatccg tttcagcacg
    20101 cggctaatca gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa
    20161 ggtccggtca gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca
    20221 tccagtggtc gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat
    20281 aacgtggcgg agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca
    20341 atgtccatca acagttcctg taactgcaaa atatcgacat tgagacgcaa tcctgccagc
    20401 ggcacctctg acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg
    20461 tattcattgg catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa
    20521 aagagaatta tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca
    20581 tacaacaaac gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta
    20641 ttaactttat ccgccagcaa gcggcaaatc tcttcacgtt tcatatcgcg taatttctta
    20701 ggaataatgc ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga
    20761 aataggcaag acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga
    20821 aaaacatcag gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg
    20881 aacaacttta atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt
    20941 ttacgcgaac aaattcctca cgatgctcgc gtattgatta cctacggcgg cggcagcgtg
    21001 aaaaaaatcg gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa
    21061 tttggcggta ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt
    21121 cgcgaacaga aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc
    21181 aaatttatcg ccgcagcggc taactatccg gaaaatatcg atccgtggca cattctgcaa
    21241 acgggcggta aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca
    21301 accggttcag aatccaacgc aggcgcggtg atctcccgta aaaccacagg cgacaagcag
    21361 gcgttccatt ctgcccatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac
    21421 accctgccgc cgcgtcaggt ggctaacgac gtagtggacg cctttgtaca caccgtggaa
    21481 cagtatgtta ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg
    21541 ctgacgctaa tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc
    21601 gccaacgtca tgtgggcggc gactcaggcg ctgaacggtt tgattggcgc tggcgtaccg
    21661 caggactggg caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac
    21721 gcgcaaacac tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc
    21781 gctaagctgc tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag
    21841 cgtattgacg ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc
    21901 cacctctccg actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa
    21961 gagcacggca tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt
    22021 atatacgaag ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt
    22081 ccagacttaa gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg
    22141 aacgtatggc taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg
    22201 gactgggcgt ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag
    22261 aagtgggtta tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca
    22321 aagccctgaa aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga
    22381 acgacgacca caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg
    22441 attatatcga cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag
    22501 catggaaagg catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca
    22561 acttccagat ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa
    22621 accagatcga acttcatccg ctgatgcaac aacgccagct acacgcctgg aacgcgacac
    22681 acaaaatcca gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc
    22741 agaaagtcat tcgcgatctg gcagataaat acggcaaagc cccggcgcag attgttatcc
    22801 gctggcatct ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg
    22861 ccgaaaactt tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc gaaattgcaa
    22921 aactcgatca gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacatgcaa
    22981 attctcccgg tgacggtaat gttccgctac cggacttttc agaaatcatt tattcccctc
    23041 gcgtcccgcc cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat
    23101 gcagagccgg aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga
    23161 ggtgaaaaac agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta
    23221 cacgcgcagc ctccttcaca gaccgtagaa caaacagtcc ggcagattta tcagaactat
    23281 aaatcagatg ccagtacacc ttatttcggt gaaaccggag agcgggcgat aacttcagcg
    23341 cgtattcaac aggcgcttac cctgaacgac aatcttacgc tgccgggcaa tattggctgg
    23401 ctggattatg atccggtttg tgattgtcag gattttggcg atctggtgct agaaagcgtt
    23461 gcgataaccc aaactgacgc cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa
    23521 gatgataaag aaaagaccac gcagacactg aaaatggtgg cggaaaatgg tcgttgggtc
    23581 attgacgata ttgtcagcaa tcatggcagc gtcttacaag cagttaatag cgagaatgaa
    23641 aaaacgctgg ccgctttagc ttcgttgcaa aaagaacagc cggaagcctt tgttgccgaa
    23701 ctctttgaac atattgctga ttatagctgg ccgtggacgt gggtggtttc cgactcttac
    23761 cgccaggcgg ttaatgcctt ctataaaacc accttcaaga cggccaataa tcccgatgaa
    23821 gatatgcaaa tagaacggca atttatttac gacaatccga tctgttttgg cgaagagtcg
    23881 ctattttcac gcgttgatga aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt
    23941 catgttcgtt ttacgctgac caatggcaac aacgaagagc aagaactggt tttacagcgg
    24001 cgcgaaggca agtgggaaat cgctgatttt atccgcccga acagcggcag cctacttaag
    24061 cagattgagg caaaaaccgc cgccagatta aagcaatgag ctgaattaaa taacaattag
    24121 ccggaacaat aaataaaagg gaacactata tgaaaacgat tttcaccgtg ggagctgttg
    24181 ttctggcaac ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat caactggcga
    24241 gcaatgtaca aacattaaat gccaaaatcg cccggcttga gcaggatata aaagcactac
    24301 gcccacaaat ctatgctgcc aaatccgaag ctaacagagc caatacgcgt cttgatgctc
    24361 aggactattt tgattgcctg cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc
    24421 ggcagcatgt cagttgccgg ggattttttt taacgtccaa ccgccgcttt agggcgtttc
    24481 ttcgcaccag cattcaccgg acgagattgc gtagacgacg ctttttttgc cgtagcaggc
    24541 gtctgacgct gggtcgccat cggcgtatgt ttagtcaacg ccggacgggt attgcggttc
    24601 tggcgacgag cttcacgcat ctcttcaatg gttggcgcag gcactaagca atcgcgacgg
    24661 ctgccaatca gatgcttttt gcccatcgct tccagcgccg ggcggattaa cggccagttt
    24721 gccggatcgt ggtaacgcaa caacgcttta tgcaaacgac gctgtttgtc gcccttcggt
    24781 acgaagacgt cttcgctctt ataaccaatc ttcgccagcg gttttttccc ggtgtaatac
    24841 atggtggttg agttcgccag cggcgatggg tagaagttct gtacctggtc gagacggaaa
    24901 cgatgctttt tcagccacag cgccaaattc accatatctt catcacgcgt accggggtgc
    24961 gcagagatga aatacgggat cagatactgc tctttacctg cctgtttcga gtaggtatcg
    25021 aacagctctt taaagcggtc atagctgccc atgcccggct tcatcatctt cgacaacggc
    25081 ccttcttcgg tatgttccgg agcaatcttc agataaccgc cgacgtgatg ggtcgccaac
    25141 tctttgatat agcgcggatc ttccacggcg atgtcataac gtacgcctga ggcgatgagg
    25201 atttttttaa tgcctttcag atcacgcgca cggcgataga ggttgatcgt cggttcgtgg
    25261 ttagtgtcca tgtgcggaca aatatccgga taaacgcacg acaaacggcg acaagtttgt
    25321 tcagcgcgtg gcgatttgca gtgcaacata tacatgttgg cagttggccc accgagatcg
    25381 gaaatcacgc ccgtaaaacc tggaacggtg tcgcggatcg cttcgatctc attaatgatc
    25441 gaatcttccg aacggctctg aataatgcgc ccttcgtgct cggtaataga acagaaagaa
    25501 cagccgccaa agcagccgcg cataatgttg accgaaaaac ggatcatttc gtaagccgga
    25561 atacgggcat tgccataggc cggatgtggc acgcgcttgt acggcagcgc aaaaacgctg
    25621 tccatctctt cggtagaaag cgggatagca ggcgggttga tccacacata gcggtcaccg
    25681 tgtttttgca tcaatgcgcg gacgcagcct gggttggttt cgtggtgcag aatacgcgaa
    25741 gcatgggcgt acagcacttt atcgcccttc actttctcga aagaaggcag caacacgtag
    25801 gttttttccc acggtttcgg gcgcggtggc tgcacggtta cggctttggc ttcctgcttt
    25861 ttcggtgcca ccggtttgtt atccgcgcac ggcaaatctt caccatacgg atgcgggatt
    25921 gggtcgattt ttccaggggt atcaagacgg ggggaatcca cgccgctcca gccaggcagc
    25981 gcctctttca cgataatcgc ggtattacgc acatcgcgga tttcactaat cggctcgccc
    26041 atcgccagac ggtgcgccac ctccaccagc ggacgctcac cgttaccaaa catcagcatg
    26101 tcggctttcg aatccaccag cacggaacgg cgcacggtat cggaccagta atcataatgt
    26161 gcggtacggc gcagactagc ctcaataccg ccgaggatca ccggtacatc tttccacgcc
    26221 tctttacaac gctgggtata aaccagtgtg gcgcgatccg ggcgcttacc cgcgacgtta
    26281 tccggcgtgt aggcatcgtc atgacgtaaa cggcgatcgg cggtataacg gttgatcatc
    26341 gaatccatgt tgccagcagt aacaccgaaa aacagattcg gtttacccag acgcataaag
    26401 tcgtctttgc tgctccagtc cggctgggcg atgatcccga cgcgaaagcc ctgtgcttcc
    26461 agcatacgac cgcaaatcgc catcccgaag cttgggtgat cgacatacgc gtcgccagta
    26521 accaaaatga tgtcgcagct atcccagcca agttgatcca tctcttcacg agacatcggc
    26581 aaaaacggtg ccggtccaaa acaggcggcc cagtactgcg gccaggagaa caggtcgcga
    26641 tccggttgga tcagggagat agagctcatt ttgcttccag aaatgataaa aaaataatca
    26701 aaggccgggg attataagcc ggaacgaaag agaaatcgaa aagtattcca tactcgccct
    26761 cctcgggcga gtatgaagat tacggtaccg gattgactaa cagttgccca atcgagccac
    26821 ggtccgccat ttccagcgtc tgactgttga agtagaacgg gaagtgcgcc caggaaggct
    26881 gaccgaaata aacaagcagc tccacttgtc cgtcaaccca aacggtatct ttccagcctc
    26941 tgtcttccgg gaacggcatc gcaccattca cgttacggat ctggaacatc acgccttcaa
    27001 tatggaacgc ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg
    27061 cggtgacatc aatacggttg acgtcccaca gctgtccatt aatacccggg tcatcaccca
    27121 gactgatatc gcgactgcga atcggcgaac cggccatgat ttcagttggc agcaagcgca
    27181 tcggaagact gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt agcaccaggg
    27241 tagaaaccag aatactggat ggctcaaaga agccgcgaat acgatcaaca atgctcgccg
    27301 cttcgccaca ggtgatcgac acttcatcgc cgttgctcat atccaccaga atctcgcggc
    27361 gctcgcccgg tgccagcgaa agttgcttca ccgatacagg agcaggcagg aatccctgat
    27421 cgccagaaat cacatgtaac gggcgaccat cgctcatctg tagttgatag cgacgagagt
    27481 tcgacgcgtt cagcagtcgc aagcgcaccc agccacgcga gacttcaacg tacgggcttt
    27541 gtacaccgtt aaccagcagc gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt
    27601 ctggcgtacc aaagttatcc agccgtttat cctggataat gaccggaaaa tcatccacat
    27661 cataatggtt ggggataggc agcgacttgc tgacttcatc ttccaccagc cacattccgg
    27721 caaggccgtt atagacctgc tgagccgtgc ggttgggagt attggcgtga taccacagag
    27781 tagctgcgtt ctggcgaata ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc
    27841 gtgccggacc gcccatcagc gggccaggta cctgtagccc ggccaccgtc attgagacat
    27901 tttctgtcag gcggttgctg taaataagct taacatcgtc gcccttccag acgcggatag
    27961 tcggccccag gtaacgacca ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg
    28021 accagtgcgc acgttgtaca gtcataaaca gcggttgccc acggcgagat tcaagtagcg
    28081 gcggaacggg tagcggttgc tgttgcccgg ctgcgctggc cttcagggga acagcgcctg
    28141 cacaaagtgc aatccccgat gcctgaatga actgacgccg actgagtgac atatttgctc
    28201 catgtaaaac tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa aatacagatt
    28261 gccgacttaa acttttccgg cggcttcgcg ttctgcgact tctttatcga gctcggcgat
    28321 tttttgttcc attatcgaac gacaatgggc agccagctca cgaacctgat ctttgccata
    28381 ctgactgacg tcaattggcg gcagcatttc gacaatcacc agaccgttgt gcagtcgatt
    28441 aagattaatc ttattcgaag ttgtagagac gcacacggga ataatcggga cgcccgccgc
    28501 aattgccgcg tgaaatgctc cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt
    28561 tccttccggg aacatccaga tggaaatacg gcgttttttg aaatgattca cgacttccgc
    28621 aatggtgccg tgagctttag tgcgattgtt tctgtcgatc aataagttgc cggttaacca
    28681 gtacaactgc ccgaagaagg ggatccacag caagctcttt ttacctaccg tcaccgtcgg
    28741 cggttgcaca atgttcgatg ctgtcaccat gtcatagttg ttctggtggt tagcgatata
    28801 gatagcattg ccgtagcttt cagcatctgc aggtttgcgg cactcaactt tcaggccgaa
    28861 cagcggcgca agacggccaa acatatgccc aaaggtggcc acatgtttcg ggttacgcgg
    28921 gctgaaaagg cagtaaatgg agccgaatac acagactaag atgctgtaaa tcacggtaat
    28981 aataagacga aagatatata gcataacacc ctctgaaggt ctgagagact ggcattgtac
    29041 gtcacctgaa atcaggttag ggcattgtta aaagccgctc tctggaaaag cgtattgtta
    29101 atcgcaacgc atgaataaac aacggtttca agggaatttt tatcgtttcc gggggttgaa
    29161 tacgctgccg gatgacgact taacgtttca tccggcgttc cttgcaagcg ggaggaaaca
    29221 ggccctcccc ggcatattac tcttcgctat caccgttgct ggcacggcga ggagagtcga
    29281 tctcaacacg atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcac
    29341 cagtgacttt ctgtaactct tccgggcgca gtttaatttt gcgtttccca acatgaatgg
    29401 tcagcgtgct ttgcggcggc agaacgtaca attgcgccag accatcttct ccacgcgcgg
    29461 cttctgccga tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat
    29521 cgcttaccgg gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag
    29581 catcttcaat caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct
    29641 tacctgcacg gttacgcgcc accagatcgt taaaggtgca gacgaaaccg taacccgcat
    29701 cggaagccat cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc
    29761 caggcggcaa cgttaatttg ccggtgagtg gttcgccctg accacgcgcc gacggcagcg
    29821 taatcgggtc gatggcatag ctacgaccgg tggaatcaac aaacactacc gattggttgc
    29881 tcttaccttt caccgccact ttgaagctat cacccgcttt ataattcagg cccggcgcgt
    29941 cgatatcatg gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag
    30001 gttcagacgg cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg
    30061 gcgaacgacg atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt
    30121 tattcatttt acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagct
    30181 cactctgctc accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt
    30241 cgaggatcgc ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg
    30301 gttcatcttc gttacgaatg atctcaatca cttcgtcgat gttgagaaac gccaccagca
    30361 aaccttccag gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc
    30421 gcacggtatc gcgacggaac accagccatt cggagaggat ttccagcagg tttttcaccg
    30481 ccggacgacc atccagaccg atcatattga ggttaatgcg atagctcttt tccagatcgg
    30541 tggtagcgaa gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa
    30601 tcaccagacg ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca
    30661 gctttttgtt gcgcatttgc gcagcaattt gctccagtac acgcgcacct gaaacctgat
    30721 gcggtaatgc gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgta
    30781 ctgaaccacg tccgttctcg tagattttac ggatctcggc gcgcgaagtg ataatttccg
    30841 cttcagtcgg ataatccggc ccctgcacga tatccagcag ctgatcgagc gtggttttcg
    30901 gctggtcgat taatgcgatt gccgcctgag ctacttcacg caggttatgc ggtggaatat
    30961 cggtcgccat gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacgag
    31021 caggcagcat tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc
    31081 cctgccccag ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac
    31141 gcattgccgc gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca
    31201 gcggataacg gtaagagaac ggctgcgcca tcaggaccat cgcttcataa caggcgatat
    31261 cgccgtgcgg atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt
    31321 tggcgctggc attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt
    31381 tcagaccatc accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta
    31441 agtaggcgtt ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt
    31501 ctgattcctc aactgattcg ccagccttca aaggggcaat attgccggag atactacatt
    31561 atctgccgcg tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg
    31621 gcgataaagg catcaatgtg ccttgatata caacgctttg ctgtagactt gatcggcata
    31681 gagggcagca caaggttgcg cccatgccac accactcggc atgggggtcc gctccgggcg
    31741 attcgagaag ttgaggttat gcgagtccag ggaagcctaa agcatcaaag tagtcgactg
    31801 taagtatatg gttcagccca gtttagctgt gtctccacca gtgccccgcg cctcctgcca
    31861 taagtgcggc ttgctcatgg gtacccccga gacggcttac ggcgcaccgg ataataaatt
    31921 tcgcgtatac atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg
    31981 gcattgttcg ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca
    32041 tcacgctaac ggcacataaa catccgtttc cagtcgctgc tccgtcaccc cttcggcaag
    32101 attggtgtag tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca
    32161 gtggcgaata atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc
    32221 cacggcataa cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat
    32281 cggttcgcta acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg
    32341 gtcgtcccag gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt
    32401 acgccacatg ataaatttcg ccacactgta gttgagcaac tcagggctac agcggtgcgg
    32461 caacatcgcc acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt
    32521 cattttgcct ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg
    32581 gatttacttt gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt
    32641 caccctgaat ctcaaccgta tctttcggcg tcaccgtcac accgttccag cgtttgtggt
    32701 cgatatcaac attgatagta ccgctggcat ctttaaacac gtagagatcg tcagagatgc
    32761 gttcaacgat attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt
    32821 ctacagtcgt tacgctgccg ttcggcccct ggaatcctcc ggcctgactt tgcgttgccg
    32881 atgggccaga aaaaccgccc tgctctgctg ccatcaccgg tgcactgcac agggccatta
    32941 ctgcgattac tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag
    33001 catttaacaa gatagtcctt aacaacttct taagggaaaa aaataaaatt taatgctgta
    33061 cagagcgcgt tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa
    33121 tgcgaatttt actgatagaa gatgacatgc tgattggcga cggcatcaaa actggcctta
    33181 gtaaaatggg ttttagtgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt
    33241 atagcgcacc ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg
    33301 atattttgcg cgaatggcga gagaaaggtc agcgtgagcc ggtactgatc ctgaccgcgc
    33361 gtgatgcgtt agcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta
    33421 aaccttttgc gttgatagaa gtcgccgcca ggctggaggc tctgatgcgc cgaaccaacg
    33481 gccaggccag caacgagctg cgccacggta acgtcatgct cgaccccggc aaacgtatcg
    33541 ccacgctggc tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac
    33601 tgatgcgtaa cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct
    33661 gggacgaaga ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac
    33721 tcggtagtga ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa
    33781 atttacccaa cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc
    33841 ggtgacctgg ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga
    33901 attgttcgac acccaactga tgctgtttgc caagcggtta agtacgctcg atctcaacga
    33961 aatcaacgcg gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga
    34021 tgacgatgcg ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg
    34081 cgataacgga gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact
    34141 ggtcggtgaa gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg
    34201 catcgttgtt ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg
    34261 gcaattgatc ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact
    34321 gggtcgtgaa ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga
    34381 ctcggaaaaa ccactaaacg cgactggcgt acccagcgaa gtgcgaccac tggttgagtc
    34441 gctaaatcaa ctgttcgccc gcacacatgc gatgatggtt cgagaacgac gctttacctc
    34501 cgatgcagct cacgaactac gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc
    34561 gcagctctct gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg
    34621 gatcgatcgc gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact
    34681 ggataacctt caggacgtcg cggagatccc gcttgaagat ctcctgcaat cgtcggtgat
    34741 ggatatttac cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca
    34801 cagcatcaaa cgcactggac aaccgctatt gctaagtttg ttggtgcgaa accttctgga
    34861 taacgcagtg cgctacagtc cacagggcag cgtggtggac gtcacgctga atgccgataa
    34921 tttcatcgtg agggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga
    34981 acgcttctat cgcccccccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt
    35041 ccagcgaatc gccaaattgc atggcatgaa tgttgaattt gggaatgcgg aacaaggtgg
    35101 atttgaggcg aaggtgagct ggtaagggta agacttttgc taaattcgca aaagactttg
    35161 cacattttgc taattttacc gtaccgctct gtgacgtact atagtcgaca aacgtctcac
    35221 cttgaggtta aaaaatgagc aacaccctga ttatcaacgg cgcgaaaaaa ttcgcccact
    35281 ccaatggtca actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg
    35341 ggcatgatgt tcgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa
    35401 actttctctg ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt
    35461 ggacagtgaa aaaatacatt gatgatgtat tcaccgaagg tcacgggacg ctgtatgcca
    35521 gcgatggccg tacccgcaaa gatccgtcaa aaaaatacgg ttccggcggc ctggtacagg
    35581 gcaaaaaata tatgctttct ctgacctgga acgcaccaat ggaagccttc accgaaaaag
    35641 atcaattctt ccacggcgtt ggcgtcgacg gtgtgtatct gccgttccat aaagcaaacc
    35701 aattccttgg catggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg
    35761 atgttccccg ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag
    35821 agctcaggct ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc
    35881 ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac
    35941 tgaaagaaga aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt
    36001 tccagtctat ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcacc
    36061 ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag cgaagccgta aaaggcgacg
    36121 tgctggagat gaatattcgt attctgcagc cagggattta atcctgcctt gtttgcccgg
    36181 ccatcctgac cgggcaatgt tctttccttt aaacctcaat ctccgccatg tcgcctttct
    36241 cttgcaacca gttgcggcga tcttccgagc gtttcttcgc cagcagcata tccatcatcg
    36301 cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg caccagacgg cgagtgttcg
    36361 gatcaagcgt ggtttcacgc aattgcatcg ggttcatttc ccccagacct ttaaaacgct
    36421 ggacgttcgg cttgcctttc ttgcgtttta attgctcaag tacgccctct ttctcttctt
    36481 ccgtcagcgc gtaataaacc tctttcccga gatcaatacg gtagagcggt ggcagtgcga
    36541 cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca
    36601 gcgtggcaat gtgcagacca tcagagtccg catccgcgag gatacagatt ttgccataac
    36661 gaagctggct cagatcgtcg ctgttaggat cgataccgat cgctaccgaa atatcatgca
    36721 cttcctgcga ggccagcact tcgtcggaag aaacttccca ggtgttaagg atcttacctt
    36781 tcagcggcat gatcgcctga tattcacgat cgcgcgcctg cttggcagat ccgcctgcgg
    36841 agtcaccttc cacgaggaac agttcggtac ggttaaggtc ctgcgcggta caatcagcca
    36901 gtttgccagg cagcgccggg ccgctggtca gctttttacg caccactttt ttagccgcac
    36961 gcatacgacg ctgggcgctg gaaatcgcca tctctgccag caactccgcc gcctgaacgt
    37021 tctggttcag ccacaggata aaggcatctt tcaccacgcc agaaacgaat gccgcgcatt
    37081 gacgcgaaga gagacgctct ttcgtctgcc cggcaaactg cggatcctgc attttcaccg
    37141 acagaacata ggcgcagcga tcccagatat cttccgccga cagcttcacg ccgcgcggca
    37201 gaatgttgcg atattcacag aactcacgca tcgcgtccaa caggccctga cgcaggccat
    37261 taacatgggt accgccctgc atcgttggga tcaggttgac gtagctttcg gtcagcagtt
    37321 caccgccttc cggcagccac agtagcgccc agtccacagc ttcagtatcg ccagcgaaat
    37381 taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat
    37441 cattcagacc gtcctgatag caccagcgct gttcggtgtt gttgatctca tctttaaagg
    37501 taatctcaac gccggggcac agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa
    37561 cagaaaatcg cgggctgtca aagaaggttt catccggcca gaagtgcacg ctggtaccgg
    37621 tattgcgttt accgcaagtg ccgacaacct gtaaatcctg caccttttcg ccattttcaa
    37681 aggcgatgtt ataaacctga ccatcgcggc gcacgttaac ttcaacgcgc ttcgacaggg
    37741 cgttaaccac cgaaatcccc acgccatgca ggccgccaga gaactggtaa tttttgttag
    37801 agaatttacc gcctgcatgc agacggcaaa gaatcaattc aaccgccggg acaccctctt
    37861 ccgggtgaat atccaccggc atcccgcgcc catcgtcaat aacttctaac gactggtcag
    37921 catgtaagat tacatccacg cgttttgcgt gacccgccag tgcttcatcg acactgttat
    37981 caataacttc ttgccccaaa tggttagggc gagtggtatc ggtatacatc cccggacggc
    38041 ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc agcgttataa gtttgcgtca
    38101 tggtttaagt tagcaattcg agttgatcgt cagagatggt gcagaccaag aaaatcgacg
    38161 atcgggttga aataatcttc gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc
    38221 tggcggcagg aagcgtagta cgccaccgcc tggcggtaat ccagcacttc atctcccgtc
    38281 tgttgcagca gccagatcaa atccggcgct tccagcgggt caatctgcat gactttgaga
    38341 tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg tgtatgggtt ctcgttctga
    38401 ccgagatagt ccgtcagcag ttcaaacggg cgcaccgccg ggtttaccac cactgcgggc
    38461 agcataaaac attgtgacaa ccaggtggcg taatatcccc ccagtgacga accgacaata
    38521 cccagcgaat cgccgccatg ttccaggaca atggattcca gcagttctgc cgcgtcggaa
    38581 ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc cgccagccag
    38641 ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga
    38701 agcgtagaca tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc
    38761 gatgcacctc ggtggtcagc gtgccatcag catgtaactc gagagtacgc cagccgggcg
    38821 cgatagtatc cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg
    38881 ttgccagcag gcggcgaccg ttccagtcga gatccagctc ctgatgaata tgaccgcaca
    38941 gcaagtattt gacgtgcgga aacttcgcca gcacggtatc cagttcgccc gcgttacgca
    39001 gactgtgttg atcgagccaa ctacaacccg caggtagcgg atgatgatgc agcagcagca
    39061 acgtatggcg ttctggcgca tcggccagtt tacgttccag ccactcaagc tgaaactcgc
    39121 tcagctcacc gtgcggcacg ccaaacacct ggctatccag caacaggatt tgccattgct
    39181 caccaataaa cacccgcttc gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg
    39241 cgggctggaa atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc
    39301 cttcagcgaa atgctgatag gccgcagagg attgatcctg cgctaaatca cctgtcgcga
    39361 caatcaggtc gaattcgtgc tgatgtggac gaatcgcctc cagcaccgcc tggtagcttt
    39421 cccaggtgtt tacccctaac agagcttcgt gcttttgtgc aaacaggtga gtgtcggtaa
    39481 tttgtaaaat cctgactctg gcctcaccag ccagaggaag ggttaacagg ctttccaaat
    39541 ggtgtcctta ggtttcacga tgctagtaaa ccggaatcgc catcgctcca tgtgctaaac
    39601 agtatcgcaa ccagtccgct aaaaactgat taatttgatg cttttcgtcg cgttgatgca
    39661 actttttatt aggataatca taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca
    39721 cttcagccac catcgcgtca tgatacagac gcaccgtcat tgacggaagg ctccagtaac
    39781 tgatcgcggg cgcagtctgt tctattgtca ccagggtagt gtatcgggtc gattccacaa
    39841 tcgtcagccg atattgtgcg tttgccacct gatagcttac agtttcgccg ggtgcgtcat
    39901 tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc gcacaggcgc atcatttcag
    39961 gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt ttttaacgct tgatgatgca
    40021 gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc tcttctaccc
    40081 actggtatgc ctgttcccgg cttaccacat gaacgcgaat atcttcgttt tcatcagcca
    40141 gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc caccataatt gacgaacgct
    40201 cactggtgcc ccccgggctt gccaggaaac ttaacaccgg tttggtccgt ttgactatca
    40261 gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc cacactttca ccctcttcta
    40321 tcatcccggc aaccatctcc agtagccagg gggtttcact ggtgtcgtat gcggcaatcc
    40381 gaatctgctc aatcagcaca acttcatcgc gcactgggtc aaagggtagc aagactgcgg
    40441 cgtgaccgcg ctcaaaaatt tcccgccgta cctcatgact catttgcccg ttgaatagac
    40501 gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa
    40561 taatttctac atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta agcattgtta
    40621 atgtcctggc actaatagtg aattaaatgt gaatttcagc gacgtttgac tgccgtttga
    40681 gcagtcatgt gttaaattga ggcacattaa cgccctatgg cacgtaacgc caaccttttg
    40741 cggtagcggc ttctgctaga atccgcaata attttacagt ttgatcgcgc taaatactgc
    40801 ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc tgagcctttc
    40861 tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc aagcacgcct
    40921 tagtaacccg gaattgcgta agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa
    40981 tgaagcgcgc agtccattac tgccacagct aggtttaggt gcagattaca cctatagcaa
    41041 cggctaccgc gacgcgaacg gcatcaactc taacgcgacc agtgcgtccc tgcagttaac
    41101 tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg
    41161 gattcaggac gtcacatatc agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc
    41221 ttatttcaac gtgttgaatg ctattgacgt tctttcctat acacaggcac aaaaagaagc
    41281 gatctaccgt caattagatc aaaccaccca acgttttaac gtgggcctgg tagcgatcac
    41341 cgacgtgcag aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag tgaccgcacg
    41401 taataacctt gataacgcgg tagagcagct gcgccagatc accggtaact actatccgga
    41461 actggcggcg ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct
    41521 gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag gcacgcttga gccaggacct
    41581 ggcgcgcgag caaattcgcc aggcgcagga tggtcactta ccgacgctgg atttaacggc
    41641 ttctaccggg atttctgaca cctcttatag cggttcgaaa acccgtggtg ccgctggtac
    41701 ccagtatgac gacagcaata tgggccagaa caaagtgggc ctgagcttct cgctgccgat
    41761 ttatcagggc ggaatggtta actcgcaggt gaaacaggcc cagtacaact ttgttggtgc
    41821 cagcgagcaa ctggaaagcg cgcatcgtag cgtcgtgcaa accgtacgtt cctccttcaa
    41881 caacattaat gcatctatca gtagcattaa cgcctacaaa caagccgtag tttccgctca
    41941 aagctcatta gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt
    42001 gttggatgca accaccacgc tgtacaacgc taagcaagag ctggcaaatg cgcgttataa
    42061 ctacctgatt aaccagttga atattaagtc agctctgggt acgttgaacg agcaggatct
    42121 gctggcactg aacaatgcgc tgagcaaacc ggtttccact aatccggaaa acgttgcacc
    42181 gcaaacgccg gaacagaatg ctattgctga tggttatgcg cctgatagcc cggcaccagt
    42241 cgttcagcaa acatccgcac gcactaccac cagtaacggt cataaccctt tccgtaactg
    42301 acgacgacga cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga tacgggttat
    42361 ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg
    42421 tatttaccac tggtcccgga agacaaaaat gaaacggaca aaatccatac gccacgcatc
    42481 gttccgcaaa aactggagcg cacgccatct gacaccagtc gctctcgcgg ttgccactgt
    42541 ttttatgctg gctggctgtg aaaagagtga tgaaacagtg tctctctatc aaaatgctga
    42601 cgactgttca gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt acaacaatgc
    42661 gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc cgtgaagact gtgttgctga
    42721 atttggtgaa ggtcagtgcc agcaggcacc agcccaggct ggcatggcac cagaaaacca
    42781 ggcgcaggcc cagcaatcca gcgggagttt ctggatgccg ctgatggccg gttacatgat
    42841 ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa
    42901 cccagccagc ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc
    42961 ccagccaggc cgcaccatga ccgtaccgaa gacagccatg gcaccaaaac cggcgaccac
    43021 tactaccgtt acccgtggcg gttttggtga atctgttgcc aaacaaagca ctatgcagcg
    43081 tagcgcaacc ggcacctctt ctcgttcaat gggtggctga taccgatgga aagagtcagt
    43141 attaccgagc gcccggactg gcgcgaaaaa gcccacgaat acggtttcaa ttttcacacc
    43201 atgtacggcg agccgtactg gtgtgaagat gcttactaca agttgaccct cgcccaggtt
    43261 gaaaagctgg aagaagtcac cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa
    43321 gtgatcgcca gcgatgagct gatgaccaaa ttccgcattc caaaacgcac ctggagtttt
    43381 gtgcgtcagt catggctgac tcaccagcca tcgctttatt cgcgtcttga tctggcatgg
    43441 gatggcactg gcgaacctaa actgctggaa aataacgccg atacaccaac gtcactatac
    43501 gaggcggcgt tctttcagtg gatctggctg gaagatcagc ttaacgccgg taacttgccg
    43561 gagggcagcg accagtttaa cagtctgcaa gaaaagctaa tcgatcgctt tgtcgagttg
    43621 cgtgaacagt atggcttcca gttgctgcat ctcacctgct gtcgcgacac ggtggaagat
    43681 cgcggaacca ttcagtattt gcaggactgc gcgacggaag ctgaaatcgc taccgagttc
    43741 ctctacattg acgatatcgg gttaggtgaa aaaggtcagt tcacggattt acaggatcag
    43801 gtaatttcca acctgttcaa gctgtatcca tgggaattta tgttgcgtga gatgttctcc
    43861 accaagctgg aggatgcagg cgtacgctgg ctggaaccgg cgtggaagag cattatctcc
    43921 aacaaagcgc ttctaccgct actatgggag atgttcccga atcacccgaa cctgctgccc
    43981 gcttattttg cggaagatga tcatccgcaa atggaaaaat atgtggttaa accgatcttc
    44041 tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca aaaccattga agcagcggaa
    44101 ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc acccgttacc gaaattcggc
    44161 gacagctata tgctgattgg tagctggctg gtgaacgatc aacctgccgg aattggcatt
    44221 cgtgaagacc gtgcgttgat cacccaggat atgtctcggt tttatccaca tatttttatt
    44281 gagtaatcaa tgatgccgga tggcattcgc catccggtaa ttgttaacct atctgcaccg
    44341 acagcatact caggctgccc atttctatac catcgaccgg aatggtaatt ggctcctgcc
    44401 catcccacgc acctaagaca tacaacaacg gcagatagtg ctccggcgtt gggttcgata
    44461 acgcgccacc ttcatggtca aggtaattca ccagaggatg ctgctctacc gccccttgcc
    44521 acgtcagatt cgctttcaca tactcattaa acgacatcgc ccaaggatat ggtgaactat
    44581 caccgtgcca cttcactgta cgcaggttat gcaccacgtt gccactggcg accaacatta
    44641 ttccttcatc tcgcagcgct gccagtttgc gccccatttc gaaatgccag gcggcaggtt
    44701 tgctactgtc gatactcaac tgcaccatcg ggatatcggc gtcaggatac atcttaatca
    44761 gcacgcccca ggaaccgtgg tcaaagcccc aggcttcttt atccagcgtc accgggaccg
    44821 acgctaacag ctcaaccaga tgctgtgcca gcacaggcga acccggagca ggataatgcg
    44881 tatcgtacag cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc gtctccatcg
    44941 cggtcactcc tgttccacgg gtaaaccagt gagccgaaac caccacaatc gcttgcgggc
    45001 gtggcaatgt catccccaac ttctgccagc tgcgggtata caaattatct tccagcacgt
    45061 tcatcggact accgtgacct aaaaacaatg ctggcatacg tgttgaagac atgatgatat
    45121 ccttaactaa aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg gagtaagaac
    45181 ccggataaca atgatgatga tcatcagtta ttttgacgat ctgcctgaag gtgaagattt
    45241 ataaggagtt gtcgatgtca gtacctctca ttctgaccat actggcgggg gcagccacgt
    45301 ttattggcgc gtttctcggc gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt
    45361 cgctaggatt tgcggcgggg atcatgttgc tcatctcatt aatggaaatg cttcctgccg
    45421 cactagcagc tgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc ttcggtctgc
    45481 ttggctattt tggcctggac cgcatgttgc cacatgcgca tccgcaggat ttaatgcaaa
    45541 aatcggtgca gccgttgcca aaatcgatca agcgcacagc cattctgctc actctcggca
    45601 tcagtctgca taacttcccg gaagggattg ccacctttgt cacggcgagc agcaacctgg
    45661 agctgggatt tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa ggtctggcag
    45721 tggcaggccc ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg tgggcgggga
    45781 tttctggact agcagaaatt cttggtggtg tgctggcgtg gttaatcctc ggtagcatga
    45841 tttccccggt ggtcatggcg gcaatcatgg cggcggttgc aggaattatg gtggcgctct
    45901 cggttgatga attaatgccg ctcgccaaag agattgaccc taataataac cccagctatg
    45961 gcgtactatg tggaatgtca gtgatgggat tcagtttagt gctgctacaa acggcgggaa
    46021 ttggttaaaa acaacatcag aagttgtcca gcacatggac aacttctgcc agcttcacga
    46081 aagccttatt ctaccttttc ggctgtctct tctctcgtac tgtttaagta tttgttccgc
    46141 gcttcaagca gtgcgtctct gtcctggcga aacgcccgac aataacgttt caaatgcagg
    46201 aaataaccca ccacaaccag agcaatcaat acaatccagt accaggcgat aaacatcctt
    46261 ttacccttaa tatttaacaa tactaaacat ttagcgcata aatttcacat atcctttttc
    46321 ggatatattc atatggtcgc ataataacgg aacaattata tgattttgat cataatgaaa
    46381 aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt aacatcttgc
    46441 atagcagggc aaaataaaaa ccgaagccag ctacaacttc ggtttcttga ttaaggcagt
    46501 aattaagcag cggttttcag ctggctttac gctcatgtgc ctgacggtat gccaccaggt
    46561 cttcaatagt cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac tctggtgcac
    46621 gcgccatcgt gccatcgtca ttagtcagct cacacagcac accagccggt ttaaagcctg
    46681 ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg
    46741 cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag atcagaaggt tttgcgccat
    46801 cggcaatcgc tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg gtagtcacac
    46861 cttcagccgc ttcaatggtc acggtaaaac cggtgccata ggcgctggtg ttattttcta
    46921 ccatcattgg cagatcaagt tgtttacggc gatcttcagt gatgcacagg caaacaatac
    46981 cgctaccgtg gcgaatggtc agcgccatct gctcaacggt catggtttct gccgggaaga
    47041 tcatatcgcc ttcgttttca cggtcttcat catcaagcac cattacaccg cgtccttcac
    47101 gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg
    47161 tctgattcat ggtaaaaaaa cctcactaaa attatggtta ccagaatcag ggcagtctta
    47221 ggagtggcgg catatagcca aaataacgtg agcgggtcca tgcccgacag aatcgttact
    47281 ctctcccatc cggactctaa ccgtcggccc cggaattaca ccggatctgc tgtcctttga
    47341 gttcgcaccc aaagcgctcg cgggctttca actgagttga tttaccgccg gtggggaatt
    47401 tcgccccgcc ctgagaataa gcggattcac tataacgcta atgattagcg gcagcaacgc
    47461 atagcttcac ataattctgg tttatgactt acccttatcg cactacaatg gtactcaaca
    47521 cctatcagta cagggaaact acaatgattg acccgaaaaa aattgagcaa atcgctcgcc
    47581 aggttcacga atcaatgcct aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa
    47641 tccgccaaac cctacaagct cagctgacgc gcctcgacct tgtaagccgt gaagagttcg
    47701 acgtccaaac gcaagtcctg ttacggactc gtgagaaact ggcgcggctt gaacaaagaa
    47761 tgagcgaact ggaaaatcgc agtactgaga ttaaaaagca gccagatcca gagactctcc
    47821 ccccaacgct gtaaacattt gtctttaatg cactgtaaat tatgaattaa tcttctaatt
    47881 tacagtgctt tctattacct tatcgcttaa ttccattcag aatatcatca caaaaatgaa
    47941 tgagtgcaat tgaaaatata attgttatgt attttttaag ttttacttaa tccatattta
    48001 gataaactaa tttcataaaa cccgaaaagt aaaatataat atttgaatga ttagcaatcc
    48061 ttaaataaca gttactaatc attcattttc tacttatatt tatataaagg aatattatgt
    48121 ctgcttttaa aaagtcactt cttgttgctg gcgtagcaat gattctttcc aataacgtat
    48181 ttgctgacga aggtcacggc attgttaaat ttaaaggtga agttatctct gctccatgtt
    48241 caattaaacc aggtgacgaa gacttaactg taaatctggg tgaagtagca gataccgttc
    48301 tgaaaagcga ccagaaatct ttggctgagc ctttcactat tcacctgcaa gactgcatgt
    48361 tgagccaggg tggaaccact tattcaaaag cgaaggttac tttcaccaca gcgaatacta
    48421 tgacgggtca gactgacctg ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag
    48481 gcgttggcgt acgtattctg gatagccaga gcggcgaagt gactttaggt actccggtag
    48541 taattacatt caacaatact aacagctatc aggaactgaa cttcaaagct cgtatggaat
    48601 ccccgggcaa agatgctacc ccgggcaacg tttacgctca ggctgactat aagattgctt
    48661 acgagtaatc acattaggcc gggttcgccc ggccacatca ttaacggatt aatgataagt
    48721 ggaacagatg tataaaaaat taaaattaac cacaataagc gaattgatta aaaatattta
    48781 ttgttcatta tccgttctca tcattggttg tgcgtcagct tatgccgttg aattcaacaa
    48841 agatttaatc gaagccgaag atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg
    48901 ccaattaccc gtaggtaaat actcattaaa cgctcttatt aataataaaa ggacgccaat
    48961 ccatctggac ctgcaatggg tattaattga taaccaaact gcagtttgtc tgactccaga
    49021 gcaattaaca ttattaggct ttactgatga gattattgaa gaggctcagc aaaacctgat
    49081 cgatggttgt taccctatcg aaaaagaaaa acaaattaca acttatctcg ataaagggaa
    49141 aatgcaatta tccatatctg cacctcaggc atggttaaaa tacaaagatg caaactggac
    49201 gcctcctgaa ctttgggatc atggtattgc tggggcattt cttgactaca atttatatgc
    49261 ctctcattat gcaccacatc agggcgataa ttcacaaaat ataagttcct atgggcaggc
    49321 tggggttaat cttggggcct ggcgcctgcg tactgattac cagtacgatc agtcatttaa
    49381 caatggcaaa agccaggcga acaacctgga ttttccgcgt atttatttgt ttcgcccaat
    49441 cccagcaatt aatgcaaaac taactatagg tcaatacgat actgaatcct ctattttcga
    49501 ctctttccat ttttctggcg tttcgttgaa aagcgatgag aatatgttgc cgcctgatct
    49561 acgtggttac gcaccgcaaa tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc
    49621 acagaacaac cgtattattt atcaagaaaa tgttcctcct ggcccatttg ctattactaa
    49681 tttattcaat acattacag
//
LOCUS       DAETQN010000014        48840 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.014, whole genome shotgun sequence.
ACCESSION   DAETQN010000014 DAETQN010000000
VERSION     DAETQN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.014"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            34..663
                     /locus_tag="K0W68_000885"
     CDS             34..663
                     /locus_tag="K0W68_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBI3072534.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="K0W68_000886"
     CDS             complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="K0W68_000886"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HBI3072535.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1933..3021
                     /gene="hcxA"
                     /locus_tag="K0W68_000887"
     CDS             1933..3021
                     /gene="hcxA"
                     /locus_tag="K0W68_000887"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HBI3072536.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(3031..3228)
                     /locus_tag="K0W68_000888"
     CDS             complement(3031..3228)
                     /locus_tag="K0W68_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBI3072537.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="K0W68_000889"
     CDS             complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="K0W68_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HBI3072538.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5696..6109)
                     /gene="entH"
                     /locus_tag="K0W68_000890"
     CDS             complement(5696..6109)
                     /gene="entH"
                     /locus_tag="K0W68_000890"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HBI3072539.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(6112..6858)
                     /gene="entA"
                     /locus_tag="K0W68_000891"
     CDS             complement(6112..6858)
                     /gene="entA"
                     /locus_tag="K0W68_000891"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HBI3072540.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6858..7715)
                     /gene="entB"
                     /locus_tag="K0W68_000892"
     CDS             complement(6858..7715)
                     /gene="entB"
                     /locus_tag="K0W68_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HBI3072541.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7729..9339)
                     /gene="entE"
                     /locus_tag="K0W68_000893"
     CDS             complement(7729..9339)
                     /gene="entE"
                     /locus_tag="K0W68_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HBI3072542.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9349..10524)
                     /gene="entC"
                     /locus_tag="K0W68_000894"
     CDS             complement(9349..10524)
                     /gene="entC"
                     /locus_tag="K0W68_000894"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HBI3072543.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            10899..11864
                     /gene="fepB"
                     /locus_tag="K0W68_000895"
     CDS             10899..11864
                     /gene="fepB"
                     /locus_tag="K0W68_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HBI3072544.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(11945..13195)
                     /gene="entS"
                     /locus_tag="K0W68_000896"
     CDS             complement(11945..13195)
                     /gene="entS"
                     /locus_tag="K0W68_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HBI3072545.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            13306..14310
                     /gene="fepD"
                     /locus_tag="K0W68_000897"
     CDS             13306..14310
                     /gene="fepD"
                     /locus_tag="K0W68_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HBI3072546.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            14307..15299
                     /gene="fepG"
                     /locus_tag="K0W68_000898"
     CDS             14307..15299
                     /gene="fepG"
                     /locus_tag="K0W68_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HBI3072547.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            15296..16111
                     /gene="fepC"
                     /locus_tag="K0W68_000899"
     CDS             15296..16111
                     /gene="fepC"
                     /locus_tag="K0W68_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HBI3072548.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(16108..17241)
                     /gene="fepE"
                     /locus_tag="K0W68_000900"
     CDS             complement(16108..17241)
                     /gene="fepE"
                     /locus_tag="K0W68_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBI3072549.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(17457..21338)
                     /gene="entF"
                     /locus_tag="K0W68_000901"
     CDS             complement(17457..21338)
                     /gene="entF"
                     /locus_tag="K0W68_000901"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HBI3072550.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(21335..21553)
                     /gene="ybdZ"
                     /locus_tag="K0W68_000902"
     CDS             complement(21335..21553)
                     /gene="ybdZ"
                     /locus_tag="K0W68_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HBI3072551.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(21556..22758)
                     /gene="fes"
                     /locus_tag="K0W68_000903"
     CDS             complement(21556..22758)
                     /gene="fes"
                     /locus_tag="K0W68_000903"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HBI3072552.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            23001..25241
                     /gene="fepA"
                     /locus_tag="K0W68_000904"
     CDS             23001..25241
                     /gene="fepA"
                     /locus_tag="K0W68_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HBI3072553.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            25416..26036
                     /gene="entD"
                     /locus_tag="K0W68_000905"
     CDS             25416..26036
                     /gene="entD"
                     /locus_tag="K0W68_000905"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HBI3072554.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(26158..26310)
                     /gene="hokE"
                     /locus_tag="K0W68_000906"
     CDS             complement(26158..26310)
                     /gene="hokE"
                     /locus_tag="K0W68_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBI3072555.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(26661..26813)
                     /gene="hokE"
                     /locus_tag="K0W68_000907"
     CDS             complement(26661..26813)
                     /gene="hokE"
                     /locus_tag="K0W68_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBI3072556.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            27253..28371
                     /locus_tag="K0W68_000908"
     CDS             27253..28371
                     /locus_tag="K0W68_000908"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBI3072557.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            28437..28685
                     /locus_tag="K0W68_000909"
                     /pseudo
     CDS             28437..28685
                     /locus_tag="K0W68_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            28750..29118
                     /locus_tag="K0W68_000910"
     CDS             28750..29118
                     /locus_tag="K0W68_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBI3072558.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            29212..29865
                     /gene="nfsB"
                     /locus_tag="K0W68_000911"
     CDS             29212..29865
                     /gene="nfsB"
                     /locus_tag="K0W68_000911"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBI3072559.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            29973..31220
                     /gene="ybdG"
                     /locus_tag="K0W68_000912"
     CDS             29973..31220
                     /gene="ybdG"
                     /locus_tag="K0W68_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBI3072560.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(31288..32664)
                     /gene="pheP"
                     /locus_tag="K0W68_000913"
     CDS             complement(31288..32664)
                     /gene="pheP"
                     /locus_tag="K0W68_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBI3072561.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(32766..35909)
                     /gene="cusA"
                     /locus_tag="K0W68_000914"
     CDS             complement(32766..35909)
                     /gene="cusA"
                     /locus_tag="K0W68_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBI3072562.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(35921..37144)
                     /gene="cusB"
                     /locus_tag="K0W68_000915"
     CDS             complement(35921..37144)
                     /gene="cusB"
                     /locus_tag="K0W68_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HBI3072563.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(37160..37492)
                     /gene="cusF"
                     /locus_tag="K0W68_000916"
     CDS             complement(37160..37492)
                     /gene="cusF"
                     /locus_tag="K0W68_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBI3072564.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(37516..38898)
                     /gene="cusC"
                     /locus_tag="K0W68_000917"
     CDS             complement(37516..38898)
                     /gene="cusC"
                     /locus_tag="K0W68_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HBI3072565.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            39055..39738
                     /gene="cusR"
                     /locus_tag="K0W68_000918"
     CDS             39055..39738
                     /gene="cusR"
                     /locus_tag="K0W68_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBI3072566.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            39728..41176
                     /gene="cusS"
                     /locus_tag="K0W68_000919"
     CDS             39728..41176
                     /gene="cusS"
                     /locus_tag="K0W68_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBI3072567.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVSKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            41913..43814
                     /locus_tag="K0W68_000920"
     CDS             41913..43814
                     /locus_tag="K0W68_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBI3072568.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            43842..44303
                     /locus_tag="K0W68_000921"
     CDS             43842..44303
                     /locus_tag="K0W68_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HBI3072569.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            44323..48734
                     /locus_tag="K0W68_000922"
                     /pseudo
     CDS             44323..48734
                     /locus_tag="K0W68_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR/RHS domain-containing protein"
BASE COUNT        11693 a        13454 c        12815 g        10878 t
ORIGIN      
        1 ttataacgaa cggataaaag gaaaacgtca ggaatggggg atactatgca acaacgatta
       61 acgcaggatt taacgcaatt tctcgccagc ttgccggaag atgatcgcat caaagcgatt
      121 aatgaaatcc gcatggcgat ccatcaggtt agcccctttc gtgaagagcc agtggattgc
      181 gttctgtggg ttaaaaacag ccaacttatg cctaatgatt acaacccaaa caacgtggca
      241 ccacccgaaa aaaaactgct gcaaaaatcc attgaaatag atggttttac ccaacccatt
      301 gtggtcacac atacggataa aaatgccatg gaaatcgtgg atggttttca ccgccatgaa
      361 attggtaaag gctcatcatc attaaaatta cgcttaaaag gttatttgcc ggtgacctgt
      421 ctggaaggta cgcgtaatca acgcatcgcc gcgactattc gtcataatcg cgctcgtgga
      481 cgccatcaaa tcaccgccat gtcggaaatc gtccgtgaac ttagccagtt gggatgggac
      541 gataataaaa ttggcaaaga gctgggaatg gacagcgacg aagtgctgcg cctgaagcaa
      601 attaacggcc tgcaagagct gtttgccgac cgtcaatatt cccgcgcctg gacggttaaa
      661 taactaaagc tggcgcaggc gttcagctgc tgccagcaac gtcgattcct tcttggcaaa
      721 acagagacga atcagtttat gtgggaaggg atcggcgcaa aacaccgaca gcggaatcgc
      781 cgctacgccg tgctcctgcg tcagccactg gcaaaactca acatcatcca gggtagaaac
      841 cgcgctgtaa tccaccagca aaaagtatgt accttcacac ggtaaaatct ccagccggct
      901 ttcatttaag gcatttacca gaatatcgcg cttctggcga taaaagtccg gtaacgcaag
      961 ataatgctca ggttctgcac gtagcatatc agcaagcgcc agctgtgccg gggtattcac
     1021 cgaaaaggtc agatactgat gtaccttgcg aatttcggcg ctgatgggcg ctggcgcaac
     1081 acaataaccc actttccagc cggtcatatg ataggtcttg ccaaatgaag aaaccgccac
     1141 tgcccgctca cgcagctgcg aatgcgccag cacactggca tggccctgtt gtgaaaagtt
     1201 gatgtgctcg tagacttcat cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca
     1261 caaagcggcg aaatcagcct gctgccagac agttgcactg gagttatgcg gagtgttgag
     1321 gatcaccagt ctggtgcgct cgcttaataa tgcggcaaat tcctgccagt caacgcgaaa
     1381 atgcggtggt tgcagtgcca tacgcttcac tattccccca gaaagcgcga tggcgggggc
     1441 gtaactgtca tagctgggat caaaacaaat cacttcatcg ccattgcgca ccagtgcggt
     1501 aatcgccgcg tataacgctt ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc
     1561 tggttgatag ccatacaaac gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac
     1621 gccggtcatg ggcgcgtatt ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg
     1681 taaatagcgc ggaccatcaa aatcaggaaa gccttgcgac aggttaatcg cctggtgttg
     1741 ctgcgccagc gcgctcatct gggtgaaaat agtggtgcca agttgtggaa gtttgctttg
     1801 tggaatcaga gggttatttg tcatttttat tgtacctgac gaagttgcgt gttgaaggca
     1861 ctataacacg atgctagtat ttggcaatca agacgtttag atgtctaaat aaaacaataa
     1921 ggacaacaca acatgcctca caatcctatc cgcgtggtcg tcggcccggc taactacttt
     1981 tcacatccag gaagtttcaa tcacctgaac gattttttca ctgatgaaca actttctcgc
     2041 gcggtgtgga tctacggcaa acgcgccatt gctgcggcgc aaaccaaact tccgccagcg
     2101 tttggacttc caggggcaaa gcatattttg tttcgtggtc attgcagcga aagcgatgta
     2161 cagcaactgg cggctgagtc cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc
     2221 gcactgctcg acaccgcgaa agccctcgcc cgccgtctcg gtctgccatt tgttgccgtt
     2281 ccgacgatcg ccgctacctg cgccgcctgg acaccgctct ccgtctggta taacgatgcc
     2341 ggacaggcgc tgcattatga gattttcgac gacgccaatt ttatggtgct ggtggaacca
     2401 gagattatcc tcaacgcgcc gcaacaatat ctgctggcgg ggatcggtga cacgctggcg
     2461 aaatggtatg aagcggtggt gctggctccg caaccagaaa cgttgccgct aaccgtgcga
     2521 ctggggatca ataatgcgca ggccattcgc gacgtattgt taaacagtag cgaacaggcg
     2581 ctgagcgatc agcaaaatca acagttaacg caatcatttt gcgatgtggt ggatgctatt
     2641 attgctggtg gtgggatggt cggtgatctg ggcgatcgtt ttacgcgtgt ggcggcggct
     2701 catgccgtgc ataacggtct gactgtcctg ccgcaaaccg agaaatttct ccacggcacc
     2761 aaagtcgctt atggcattct ggtacaaagc gccttgctag gtcaggatga tgtgctggcg
     2821 caattaaccg gggcgtatca gcgttttcat ctgccgacca cgctggcgga gctggaaatg
     2881 gatatcaata atcatgcgga gatcgacaaa gtgattaccc acaccctgcg tccggtggaa
     2941 tacattcatt acctgccagt cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaaa
     3001 gtggaatcgt ttaaagcctg acgtgcaaat ttagcagcag cgcgcgccgc cttttccacc
     3061 gtagcgcgcg tcctgccgct cacggaaaaa ctcttcgtag gtcatcggcg tttgatcggg
     3121 atggttaacc cgcatatgtt cgacatagtt gtcgtaatca ggcataccaa tcatcagctt
     3181 cgccgcctga cctaaatatt ttccggcttt tgccagtgaa tcaaacatct tggcctccgc
     3241 taaccatgtt gctgaaagag tgccggatgc ggcgtgaacg ccttatccgg cctacaaatg
     3301 ctatcaacgc gctcggtcag tcccctcgcc ccatcgggga gagggttagg gtgaggagga
     3361 accctcggca ctatgccaac attcgccaac atcccccctc actctgactt tagtgtgcgc
     3421 cttttgcctg cgccacgatc tcctcgacat tttccggcat tggctcatac ggcgtttctt
     3481 tcgccgttgg cttcggatct ttcaatgccg ccagtgccga cttaatcgag aacagcgcca
     3541 gaaccaccac gaccaccata aagaagatgg ttaacccggc atccagacgg ttgttgaaca
     3601 ccagttgcgc cagctgtgac tcagtatact gcgacggaat attgccgctg tctatcattg
     3661 cctggaactt attagcaatg gccaggaagc cgactttcgc atccgggcta aacgctttct
     3721 gccagcctgc ggtcagggta caaatcagca gccaggccgt tggtaccagc gccacccagg
     3781 cgtaacgttg acgtttcatc ttgaacaaca ccacggcaca gagcatcagc gccatccctg
     3841 ccagcatctg gttggcaata ccaaacagcg gccacagagt gttaatgccg cccagcggat
     3901 cgaccacgcc ctgatggagg aagtagcccc acgccagtac gcacagcgct gttgccagca
     3961 ggttagcagg cagtgaatcg gtccgcttca ggccaggaga caccacgccc agcagatcct
     4021 gcaacataaa gcgcgcagca cgcgtacccg catctaccgc cgtcagaata aacagagctt
     4081 caaacagaat ggcgaagtga taccagaacg ccacatccat cataccaccc agcgcaccgt
     4141 gcaggatgta agccatcccc accgccagcg tcggcgcacc gcccgcacgg gaaatgatcg
     4201 actgttcacc cacttcgctg gcaatctggt tgagcgtatc cggagtaatc gcaaagcccc
     4261 agctactcac cacctgcgcg gcagaagcga ccacatccgc cgtccctgcc ggagccagca
     4321 ccgccatcgg gctgttcatg gcgaagtaca cgcccggatc gatgatacag gcggaaacca
     4381 gtgccataat cgccacgaag gattccatta acattccacc gtagccgata aagcacgcct
     4441 gcccttcgtt cgccagcatc ttcggcgtgg tgccggaaga gatcagcgca tggaagcctg
     4501 acaccgcgcc acaggcgatg gtgataaaca ggaacgggaa cagattaccg gtccataccg
     4561 ggccagtgcc atcgacaaat ttggtcagcg caggcatggt cagcgtcgga cgcataatca
     4621 aaatgcctac cgccagacca acgatagtcc cgattttcag gaaggtagag aggtagtcac
     4681 gcggggccag cagtaaccac accggcagca ccgccgccac aaaaccgtag cccaccagca
     4741 tccaggtcag ctgcacgccg gtaaagtcaa agtacggtgc ccaggtcggg ctttctgcca
     4801 cccagccgcc agagataatg gcaaaaataa ggaacaccag gccaatcact gacacttcgc
     4861 caatacgccc cggacgcaga tagcgcaggt agattcccat aaaaatcgcc agcggaatgg
     4921 tgaacgcaac ggtatacgtt ccccacgggc tatgagtcag ggctttcacc acgatcatcg
     4981 ccagtactgc aaggataatg accatgatca taaagcaggc taccagcgca atcaccccgg
     5041 cagttggccc catctcttct ttgaccagct cacccagcga gcgaccgtca cggcgcgtag
     5101 aaacaaacag caccatgaaa tcctgcaccg caccggcgag aaccaccccg gcgagcagcc
     5161 agatcatccc cggcaggtag cccatttgcg ctgccagcac cggccccacc agcggccctg
     5221 ctccggcaat ggccgcaaaa tggtgaccga acagcacttt cttgtccgtc ggcacatagt
     5281 ccagcccgtc gttatggcgc accgctggcg tcatacgcgt cgggtcaacc gccagcacat
     5341 ttttggcgat atacagccca taaaaacggt aagcgatcag atagatacag accgacgcca
     5401 ccacaatcca cagcgcgttg atctgttccc cacgatttaa agcaatgtat cccagagcaa
     5461 atgctcccat tacagagagc actgtccaga cgaggtattt ccctgatttg ttcatagttg
     5521 ttatccgtgt gcgtgtccat agagatgtta cattttgttt ctataacatc tttgtacgat
     5581 ttaacaacac ggtaaccatg taaatcggag ttgaaaccaa tatttaactt aacaatgtta
     5641 actcgatcac tccgttaacc gagttttcct gcaatctcat ttaattctgt cgggctcatc
     5701 ccaaaactgc cgtacccagc cgacaagtgc agcaacgccg cccctgttca tcgaaaacga
     5761 tgatttccca gctctgattt tgccgcccaa gatgcagcgg ctggcagacg ccgcgtacct
     5821 tgccttcaga catcgggcga tgatgggtgg cgttaagttc cgtacccacc acacactgcc
     5881 cgtcacgggt catcataaat ccggccatcg atcccagcgt ttcagccagc gccgccgacg
     5941 cgccgccatg cagcaggcca aacggctgat gagtacgggt atcaaccggc atttcggctt
     6001 ccagcacatc atctcccaga cgggtataca caattcccag atgcgccacc attgtgttat
     6061 cgctggtggc gttcagttcg tcgagcgtta aatggcgttt ccagatcatg cttatgcccc
     6121 cagcgttgag ccgccatcga ccacaatatc ctgtagggtg atatggctgg cgaggtcaga
     6181 ggcgaggaac aaaatcgtgt tggcgatctc ttgtggacgg gcgattttcc ccagcggaat
     6241 gccgagttta aactgctcgc caaagccgcg aatacgctgt tcttcggcgt catcgctcac
     6301 ccacagcgtg cgttgcatat cggtgtcggt ggagccaggc gaaaccacat tacagcgcac
     6361 gccgctaacc gccagttcca gcccgacgct caacgccagg cttttcagcg ccgctttcga
     6421 tgcgccataa gcactcatac caatacgcgg cgtgtgtgcg gcgtcggacg ccacagtgac
     6481 aatcgccccg ccccgctgac ggcgaaactg gttcatggtt tgctgaaaca ggttaaacgc
     6541 accgccgacg ttaaccgcaa aagtctgctg ccagtcctct ttactgagct gatcggtcgc
     6601 gcccatgcgc aaaattcccg ccgcattgac cagcacgtcc agtcgctccg tttcagctaa
     6661 cagtcgctga catacctgcg cgacctgcgc agcatcggca acatccatca cttcggtcgc
     6721 aaagggatat tgctcctgag cgaacgcttg atcaaaaccg gtaactttcg ctcccgcttc
     6781 aacaaacgcc agcgccgtgg cgtagccgat acctttacct gcgccggtta cccagacatt
     6841 tttaccgctg aaatccatta tttcacctcg cgggagagta gcttccacca ggcgtcgatg
     6901 gtcgggtttt tcgccagcat gacaaagtcg atatcgccat gcactttgcg ccagcgcgcc
     6961 gccagcgcca tcatgcgcac cgaatccaga ccgtagtcga tcaggttgtc gtcatcgaac
     7021 ggttcatcgg actcgtccag caacggcagg ataacctcac gcagcgccgc tttcctggcg
     7081 gggacaggtg ctggcagtaa tccttcagtc atcaccaccc ggccagaccg cccggccaca
     7141 tatttcagcg acatcaaatg ctcgtcacgg ctgaaatcgg ccagcgcatc cgccaccata
     7201 aacggtttaa tatcgcgcat aaatgcgtcg gttgcggtgg tcatacagcc aatgtgggca
     7261 tataccccgg taataatcag ctggttacgt ccactctctt tgagcatttg ctccagcgga
     7321 gaacgatgaa acgcgctgta gcgccacttc accagcaccg tgtcgtcggc atctggcgtc
     7381 aggcgatcca ccaccttttg ctgttccggc gaacgggtca ggcccggccc ccacatatca
     7441 ttcaacagcg cccgatcttc atcgctctgc tctttcggct gggcggtgta ataaaccggg
     7501 atattgtgct gtttgcagta gtcgcgcagc gcagcaatat tcgcgatcac ctgctccatc
     7561 atcgggcagt tctcgcccca gaagctgaca aaatagtcct gcatatcatg gattaacaac
     7621 gcggcacgtt gcggttcaaa ggcccagtca actttattct gcggaatatc gtgagactcc
     7681 ggcagtgcgt aagcctgtaa ttttggaata gccatcgtgt tctctccttc aggctgatgc
     7741 gcgtgacgcc agccactgac gtaattgttt tttatcgact ttcccgaccg ccgtcagcgg
     7801 aagtgaatcc acacactcca cgcgatccgg taatttaaat tcggcaatac cctgttcacg
     7861 caggaaacga cgcacctgca ccgcgcgcag cggctctttt accaccagat aagcacagct
     7921 tttttcgccc atcagctcat cttccatgct caccagtgcg gcatagatca ccgccggatg
     7981 gcgcagcagc aggttttcga tctcttcggc agcgatcttc tcgccgccac ggttgatctg
     8041 atctttctcg cgcccctgca cggtgatgta accctctgga tcaatagaga tcagatcgcc
     8101 ggaacagtaa aaaccgttgg catcaaaggc actggcattg tgctgcggac ttttgtaata
     8161 accgcggaag gtgtacggcc cgcgcgtcat caggcgtcca acttcccctt gcggcagtgg
     8221 atttccttca gcatcagcaa cccatacttc atcgtccgga cacattgggt aaccctgggt
     8281 atggataatt ttctccgcgc tatcatcaag tcgggtgtag ttcaccagcc cttccgccat
     8341 gccaaacacc tgctgcaact ggcagccaat ctcagcggaa atacgcgccg caagggtggc
     8401 agaaagacgt gcgccgccga cctgtaacag tttcagcgag gcaagctgcg cccggctttc
     8461 gccttcagcc agcgcctgca accacaggct aactgccggc ggcaccagcg cggtaacgtt
     8521 aacctgatgt tttgcaatca acgggaagca aagcgtggcg ctgggatcgg ccgccagaac
     8581 aaccgttcct ccggcaagaa agacgcccag cgatcctggc gaactcatag cgtagttatg
     8641 agccgccggg atcgcgcaca ggtagcgtgt ctgttgtgtg aactgacaaa tctcgacgct
     8701 acgacgcacg ctgtagtagt agtcgttatg agtgcgcggg atcagcttcg gtgtaccggt
     8761 ggtgccgccg gaaagctgga aataggccac ttcatcagca ggtgatggcg tagcagtaaa
     8821 accgtctgcc ggatggttaa tcgcatcctg caagttatgc tcaccgctgt cgttgagcag
     8881 ttgcaccacg cgaatggagg aatgttctgt gacgaaagta ttgaggaaat catccccgct
     8941 aaacagcgca tgttggcgat cggcaatcag taacgccggt tcaatctggc tggcataggc
     9001 gttcagttca ctacgctgat ggctgaacaa cgccagcacc ggcgcaacgc ccagtttcag
     9061 cagtgcgaaa aaggtaatgt aaagttcagc gacgttaccc agttgtacca gcgcggtttc
     9121 accgggttta atgccctgac ggcgtaaact acacgcgagg ttatccgccg cctgattcag
     9181 ctcccggtaa ctcaactgtc gctcgccgtc gataaccgcg atgctgtcac tcgcagcgtg
     9241 gcgagtcaga atgtcggtca gcggcaaatc ctgccagtag cctttttccc gatagcgacg
     9301 ggcaaactct tccggccagc gggtgaatgg aatgctcatc ctcgctcctt aatgcaatcc
     9361 aaaaacgttc aacatggtag aaagtttgac gcctgtttcg cgccactcac ccaacggtga
     9421 cgacgcaggc acaatccccg ctccggcaaa cagacgcacc tgattttccc gcagcttcgc
     9481 gcagcggatg gtcaccaccc attcgccgtt accttcgctg tcacaccaac ccacaatgcc
     9541 gccaaacagt tcgcggtcaa acggctccag ttcagcaata acctgggtcg cggcctgatg
     9601 cgggaaaccg ctcagcgcgg gggtcggatg cagcagacag gccagagtca gtgcgttttc
     9661 ttgcgaattc gctttacctt caaagggagt tgcgagatgc cacagcgtcg gcgtggtaat
     9721 caattgtgga gaggagggaa cgtgtaactc actactgcgt tcgcgcagta cctctttcat
     9781 cgcctgagtc accagttcat gttcatggcg atctttttct gacgccagca gacgattacc
     9841 cgcttcgcga tcgagcactt catccggctg acgacgcgcg gaaccggcta acggaatgga
     9901 gctaaaacgc tcgccgtctt tacgtagcag cagttccggg ctggccccca gcaggacgcc
     9961 accatcagcc agcgggacat ggaagttgta actaaccggg ttttgcgcaa tcaaccgttc
    10021 cagcaatacg ccactatcaa tggcggcgtc agtggtgata tcaatcaatc gtgacaacac
    10081 cactttgtcg acctgcggcg tggcggtaag tgcggcggcg cgggcaacca tctgttcaaa
    10141 cgtggtttgt tcaggaattg cctggcgttc caccacgttc agcgactggc tgcgggtaaa
    10201 acagtgggct gaggtctgtt tttcctgacg ggagaacgac tgccaggatt cgggaatata
    10261 cagcgacgaa ggctgacgtg gatcgaaggg aatcgccccg accatcaccg gatttttgat
    10321 gccctgcgct ttggcatcgg caaacagcgc ggcgagtttt tgctggaagg gactgtcggg
    10381 cgaatccccg ttcacagccg gttcatcgaa gcgggcgaaa catcctgacg tcgtaaaact
    10441 gcggtacggc gacataaaga aaaagcgatt gggcgcaagt gttgccatgg tctgctgtac
    10501 ttcctcagcc agtgacgtat ccatatcatc ctccacaaaa tgataaaggc tttaataatg
    10561 attatcattt atattttcgg tcgctaacct aaaagcaaac gcgcactatg tcaactcttg
    10621 aggtaacgca atctgcaact cggggttgca tctggttgtg gttacaatga aaatgagaag
    10681 cattattgat gaattcgcat aagcgcaatg tgatgtcctg cgccgttttg ccccctctcc
    10741 cttccagggt gagggctggg gtgagggtta atgttcgcac cagtgctggc tgttcccctc
    10801 accctaaccc tctccccaaa ggggcgaggg gacggattgt gcgctttgtc gaatttgtca
    10861 ttacgccctt aaccttatta ataacaggaa gctgacttgt gagactcgcc ccgctctacc
    10921 gcaacgccct tctattaaca ggacttttgc tttcaggaat agccgtagtt caggccgccg
    10981 actggccgcg tcagattact gacagccggg gcactcatac cctggaaagc cagccgcagc
    11041 gtattgtttc caccagcgtc accctgaccg gatcactgct ggcgattgat gctccggtga
    11101 tcgccagcgg cgcgaccacg ccgaataacc gcgtcgcaga tgaccagggc tttttacgcc
    11161 agtggagcaa ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc gaaccgagcg
    11221 ccgaagccgt tgccgcgcaa atgccggatc tgattttaat tagcgcaacc ggcggggatt
    11281 cggcgctggc gctgtatgat cagctttcca ccatcgcccc gacattaatc atcaattacg
    11341 acgacaaaag ctggcagtcg ctgttaacgc agcttggcga aattaccggg catgagaaac
    11401 aagcggcaga gcggattgca cagtttgata agcaactggc agcggcgaaa gagcaaatca
    11461 aattaccgcc gcagccggtc actgccattg tctataccgc cgctgcacac agtgccaatc
    11521 tctggacgcc ggaatcagca caagggcaga tgctggaaca actcggcttt acgctggcga
    11581 agttgcccgc agggttaaac gccagccaaa gccagggcaa acgccatgac atcattcagc
    11641 ttggtgggga aaatctggct gctgggttaa acggtgaatc gctattcctg ttcgccggtg
    11701 atcagaaaga cgccgatgcg atttatgcca atccactgct cgcgcacctg cctgcagtac
    11761 aaaacaagca ggtttatacg ctgggaaccg agacgttccg cctggattac tacagcgcca
    11821 tgcaagtgct ggacagactt aattcactgt taaaaaaaga ttgataaaac atacaattag
    11881 agcactaata atcccctcgc ccctttgggg agagggttag ggtgagggga aaatgcacct
    11941 ttagctaact gtcagacgct gtcacctgcg gcggcgtctg gcgaaaacgt cgcaactcca
    12001 ccagcaccag caataacaac acgccgataa tcaacaaacc aaaaccgctc gcgcttgcgg
    12061 aagcaaccgg tgtcatcatc gcgcccaaac cacccaacag cgccgcgcct atggcatcgc
    12121 ccgtcacgtt ctgcgccgtc cacaaaccgt taatccgccc taacatcgct tccggggttt
    12181 gcgtttgcag cattgtatat tgcagcaacg aactaaccgc gctcagccag ccgaacagcg
    12241 ccagacaaac cacgcctaaa atccacatcg gcatcaggcc aaacagacca atggcgagga
    12301 acgatcccag cgtggagagc agcatcaata accctggtcg cgcactatgt gccagcttcc
    12361 cgctggttaa cgcaccaata gccgcaccga gcgggatcgc cgcatagaga aaaccaatct
    12421 gtgccgccga catctgccag ttgtcagcca gcgccggata cagtacccgc accgcgctcg
    12481 ccatcgtcaa taaaccaccc agcaacgcaa tccctcctac cagcgggctg gcgagcagaa
    12541 aacgaaatcc tgccagtaat gatttcaacg gatgctcgcg cggctgcggt ggcggtggca
    12601 acgccggaag gcttaacaac ggtagcaagg taataaacgt gcccgccgcc gccagcccgt
    12661 agttccaggc tacgccgccg gtcgccagca ataaaccgcc aatcatgggc gaaatcaccg
    12721 accccagacg cacggtcaac atggtgatcg ccccggcctg cattaagttt tcacgcccta
    12781 ccagtgcagg tgttgccgcc agtagcgccg taacaccaag tgatgcgaaa aaaccatccc
    12841 ataaaccaag taaatagatt gccagcaatg acggctccgg cagcagcgcg ttcaggcaca
    12901 gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa aatcactttt ttgcgctcat
    12961 agcgatccgc cagcacaccg ccaaccatca ggccaacaaa catcgcgccg ccggtcagcg
    13021 tcaccgaaag ccccacctgc caggtagaat gcgtcatcat ctggatctgc accggcaccg
    13081 cgacgccgag caaacccaga gacacaattg agataaaacg agcgaggaat actgcgcgaa
    13141 acgccgggtg cgttttcaac aggctgaggt taagcagcca ggattgttta ttcattacaa
    13201 tgccttgcca tcaaatattt taaataccta ttcttaggct gcacatgcta acatatccaa
    13261 ataagatcga taacgataat taatttcatt atcatggaag ttcgtatgtc tggttctgtt
    13321 gccgtgacac gcgccattgc cgtgcccgga ttgctgttat tactgattat cgcgacggca
    13381 ttaagcctgc tcattggggc aaaatcactc cccgcgtcag tagtgctgga ggccttctca
    13441 ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg cgcgactgcc gcgcaccctt
    13501 gccggtttac ttgcaggcgg tgcgcttggg cttgctgggg cgttaatgca aaccctcacc
    13561 cgaaatccac ttgccgaccc cggcttgctt ggcgtgaacg ccggagccag ctttgccatt
    13621 gtgctgggtg cggcgctgtt tggttactca tccgcgcagg aacaactggc gatggccttc
    13681 gccggggcgc tggtggcctc attgattgtt gcctttatcg gcagtcaggg cggcgggcag
    13741 ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg cggcggtgct ggaagggctg
    13801 accagcggca tcgccctgct taatcctgac gtctacgatc aactgcgttt ctggcaagcc
    13861 ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg tgctgatccc ggttctgatc
    13921 gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca gtttgagcct cggcagtgac
    13981 accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt tgattggtct gctggcgatt
    14041 accgtgcttt gtggtagtgc gacggcggta gttggcccga ttgcctttat cggcctgatg
    14101 atgccgcaca tggcgcgctg gctggtaggt gccgatcatc gctggtcgct gcccgtcacg
    14161 ctacttgcta cccctgccct gctgctgttt gccgatatca tcgggcgggt gattgtgccc
    14221 ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg caccggtgct gatcttcctc
    14281 gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc tcgccgatta ctcatcacct
    14341 gtttgctgct gatttccgcc tgtgtggttg caggtatctg gggattacgc agcggtgccg
    14401 tcacgctgga aatctcgcag gtattcgccg cgttgatggg cgatgcgccg cgcagtatga
    14461 cgatggtggt caccgaatgg cgtttaccac gcgtgctgat ggcgctgttg attggcgcgg
    14521 cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg taacccgctc ggcagccctg
    14581 acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct ggtggcgatg gtgctgtttg
    14641 gtcaggacct gacggctatc gcgctggcag caatggtggg cggcattgtc acttctctgt
    14701 tggtctggct gctcgcctgg cgtaacggta tcgacacctt tcggttgatt attatcggca
    14761 tcggcgttcg cgccatgctg gtggccttta atacttggct gttgctgaaa gcgtctttag
    14821 aaacggcgct aacagcgggt ttgtggaatg ccggatcgct caatggcctg acgtgggcaa
    14881 aaacttcgcc ttccgcaccc atcatcatat tgatgctcat tgccgccgcc ttgctggtgc
    14941 ggcgaatgcg cttgctggag atgggcgatg ataccgcctg cgcgctgggc gtcagcgtcg
    15001 agcgttcgcg tctgttaatg atgctggttg cagtggtgct taccgctgcg gcaacggcgc
    15061 ttgccgggcc gatttccttt attgctttag tcgcgccgca cattgcccga cgcattagcg
    15121 gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg agcgctgtta ctgctggcgg
    15181 ccgatctctg cgcccaacaa ctgtttatgc cgtatcaact tccggttggc gtcgttaccg
    15241 tcagcctcgg cggtatttac cttatcgtct tgttaattca ggagtctcgc aaaaaatgac
    15301 cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg ggatatggca aatataccgt
    15361 tgcggaaaat ctgactgtag aaattcctga tggtcacttc acggcaatta tcggaccaaa
    15421 tggctgtggt aaatccacgt tactgcgtac cttaagccgc ctgatgacgc ctgctcacgg
    15481 gcatgtctgg ctggatggcg agcacattca acattacgcc agtaaagaag ttgcacgccg
    15541 gattggtctg ttggcgcaaa atgctaccac gccgggcgat atcaccgtgc aggagctggt
    15601 ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc tggcgcaaag aggatgaaga
    15661 agcggtaacg aaagcgatgc aggccacgag aataactcat ctggcagatc aaagcgtgga
    15721 taccctttct ggcggacaac gccagcgagc gtggatcgcg atggtgctag cccaggaaac
    15781 ggcaattatg ctgctcgacg aacccacgac ctggctggat atcagtcatc agattgatct
    15841 actggaactg ttaagcgaac tgaaccgcga gaaaggctat accctggcgg cggtgctgca
    15901 cgatcttaat caggcctgtc gttacgccag ccatttgatt gcattgcggg aagggaaaat
    15961 tgttgctcag ggagcgccga aggagattgt cactgctgaa ctgattgagc gcatttatgg
    16021 tctgcgatgc atgatcattg acgatccggt agccggaacg ccgcttgtgg tgccgctcgg
    16081 acgaacggca ccttcaaccg caaatagtta aactaagtgg tctgccatca tggcatcctg
    16141 ttttctggat gccatcgcat agcgcaataa cacgctacca caagccacca tcccgccgat
    16201 cagcgcggaa aggatcacaa taatcgcctt acccggaccg tcttttttca ctggcaatga
    16261 cgggcttaac tgatatttaa acggcgtaaa attcacatcg ttgacatttg cttttgttaa
    16321 ttgctcgaca agatactgcc gattacgtaa ttcaccgttc agttccgcaa cgtcagtaac
    16381 cgctttttca atttccagtt tgcgttcaat accgtctgcg ccgagagaaa tagagaaatc
    16441 ggggtcatct ttaacggcct gaccattact gtatacgggc tttttaattc ctgccgcgtt
    16501 ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca tcaagttgat ttttcgtttt
    16561 aatgcgatcc tgagccagtt tttctttttc aaactgggtt ttgatctcca gtttattacg
    16621 gacgttttct atcgactctt tcaccaccaa cgcagagata taatcgatat acccgctcaa
    16681 aacggtctgc gcctctttac tggttggcgc ggtaaaactt agcgtccagg aggtatacag
    16741 tgacggttca tcttttttct tactggcatt gtcatcaacc gctttcattt tttcgctcaa
    16801 tgcaacaata gcgcgatgca aatccagttc atcgattttc gcctctttta attggtccat
    16861 cacataaggt gatgaacgca ggtactcttc cagcaagcta acagactgaa acttcttgat
    16921 aaacaggtta aatgcaccgc cacggtcgat attaacatcg agatcaagaa cgcggagttt
    16981 ggtaaaggtt ttctccaggt cctgccactg aatggcttcg gcgggagtaa taacggctga
    17041 gctggtccat ttttgcggca ggatgaaaga gatcagtaaa cctgcgcagg caaatgcaaa
    17101 aacgaccgcc atgaccgttt ttttggcccg ccataaaact tcgattagat taagtaaatc
    17161 aatttcatta ttactgggcg acgccagagg atactcagaa aaatgagcgt cacttccctg
    17221 tttaatattc aatgatgaca tggggtataa cctgaaaatg agtccaatag gggaaaatct
    17281 ccacggcgct cactttacca gttggtgaga attatccgaa gctgaagttt gtaaattcct
    17341 tccactgaat gcaatattca ctctatttaa gagaaatatt catcaaacct ataaatcaaa
    17401 caaataaaaa acccataatt attttctgta attatgggtt tatagataat attaatttac
    17461 ctgtttagcg ttgcgcgaat aatcggccca atttttacaa acgcccctgg agagataata
    17521 tccacatgcg cacaatcctg acgatagata tccagctccg caatccacgg cgaccaggcg
    17581 cgttcaggac tcataccttc ctgaagcgtg cgttcagcaa caaacaacgt cgctttaccg
    17641 tcaaacggta cgctatgagc agtcgtcagc aggcgcacag catcagcgta gttgccttca
    17701 atggtggtaa acaactccgt tgaagtactt ccctgctgtg ccgccaggaa ggcctcgcgc
    17761 tcgcggttaa tctccgccag cacttccggg tccagaccat tagcttcttt ttcctgccag
    17821 ttttgcgttt ctggcggcca ggtatccagc aagccaagaa atgccacctg ttcgccacgg
    17881 gcacgcagtc gcgccgcaat accctgcgcc agcgtaccgc caagggaata ccccagcagg
    17941 taataagggc cgtgcggttg ttgctcaagt aacgttgcca gatgcgcttc gcagacttca
    18001 tccaggtttg tcgctgtctg catgggtcca tgagggcgcg gcgactgaat gccaataatc
    18061 gaccattgtg gatcgagata acgcgagagt acgctaaact gccaggcaaa accggacgcc
    18121 ggatggaaac aaaacagcgt cgggccatta ccttcacgca acggcagaat ggtttcgaat
    18181 cccatgcgcc gggagctgtc ctcttcacca tcaataatcg ttgccagttt ggcgacggtt
    18241 gacgcgacca tcacctgccc cggcgtcacc tgacgagcaa actgtcgact taactgcgct
    18301 gccagtttca ttgccagtag cgaatgaccg cccagcgcaa agaaatcagc atcggcatcc
    18361 tgcacgtcac aacccagcaa cgacgcgaat gccgcggcga taatcgtttc actgcccgct
    18421 ttcggtgcac gtcccgacgc ttgtgtcttc agttcaggca acggtaaggc tttgcgatcc
    18481 agcttgccgt tggcgctaag aggtaactgt ggaagttgca gcagaaccac cggtaccata
    18541 tgtggtggca atgtttcacg aagctgcgcc tgcaatgcgc tggtatccaa cggcaggccc
    18601 gattgcgaca ccagatagcc caccaactga cgcgcatcac caccagtggc tgccgcctgg
    18661 ttaatcacac aggcgtgggt aacggcttgt tcgacatccg gcagcgcctg catcacgcga
    18721 tcgatttcgc ccagttcgat acgctgcccg cgaattttta gctgatcgtc actgcgcccg
    18781 aggtactcca ccgcgccgtt atccagccag cgggcaacgt ctccggtacg gtacatccgt
    18841 tcgccaggag caaaaggatc ggcaataaag cggctggcgg tcagatcggg gcgtccgaga
    18901 tagccctgcg ccagttgaat gccggtaaga tagagatcac ccgccacacc cggcggcacc
    18961 ggatgcatca tcgcatcaag aatacgcagc cccatgttcc acaccggata accaatcggc
    19021 acactgctgc cgcgcacctg tgccagttcc tcgccaaaag ccggatacca gctgacatct
    19081 accgccgctt ccgtcgggcc atacagatta tgtaacggcg caccggttaa ctgttgccat
    19141 tcacggcata aatcagccgg taaggcttca ccactacaga aaacctgttt caacgtcgcg
    19201 caactctggc gagcggtttg cggagtcagc gaggcaacaa atgccgccag catcgacggc
    19261 acaaagtgcg tggtcgttac gccatattcg gcaaagaatt gctgcatagc gagcgggtca
    19321 cggtgcgctt ccggttcagc catcaccagt tttgcccctg cgataaacgg ccagaaaaac
    19381 tcccacaccg agacatcaaa actgcacggc gttttttggg caacgacatc ttcgcctgta
    19441 agcgggtagt gattttgcat ccataacagg cggttaacga tagccgtctg cccgaccatt
    19501 acccctttcg gcctgccggt ggagccagag gtaaagatga tataagccgt gtggtgcggt
    19561 tgtgaaagtt gcagcggcgc actgccctgg ggggtaagcg gggcgttata gcaaaggctt
    19621 gttaaattgg gaacatcgct aaagcgcggc agttgatcgt cggtggtaat taacagcgac
    19681 ggacgcgcat cttccagcat cattttcagg cgatcgtccg gatagccggt atccagcggt
    19741 agccaggccg cgccagcttc aactattgca tggagtgcca gggtcaaaaa gaccgagcgc
    19801 ggtagtgcca ccgccacgct gtcccctggt ttaacgccgc gctcacgcag cagattcgcc
    19861 agcgccacca cctgctcgcg catttcccga tagctgaaca ggtaacgcgc atctgccagc
    19921 gccggagcat ccggtgtttt tgccgcttgt tctgccacca gcgcgctcag cgtggtttct
    19981 ggaatctcaa cctgagtggc gttgatctgc gccagctgcg catactcacc tggcagcata
    20041 atatcgacat cgccgcacaa cagcgccgga tccgcggcaa actgggcaat cagcattttc
    20101 aggcgttcag catgctggat taacgttggc tcatcgtaac gctgtttatt ggcgaggatc
    20161 tcaatactca aatcaccgtg ttcatccggg aacagggcca gttcaaggtc attaaccgga
    20221 ccggttgcca gggtatgggt ttgcgcctga acaccaggaa tatccagttg gtaatcaaat
    20281 accttaatat tgagtaccgg accaaacagc ggttcatcac ctgccgctcg cccgctgtca
    20341 cggacaattt gttcggcatc gtaacgttga tggcgacgca tttttttcag ttgagccgcc
    20401 agtcgggttg ccagttccgg cagcgtttct tgcgccgcaa tgtgaatacc caacggtaaa
    20461 acgttgagca cgggtccgct agccgtcagc gccgccgagc ccagtcgacg cataaagata
    20521 aatccggcgg catagtccat gcgattgcac aatcgcccca gccacaaggc tgccagcgca
    20581 agggctaaat cggtacgctg cacacctgaa agttgcgtag ccagctggcg gaattccccg
    20641 tcggtaaatt ccagtttcag gcgcagaata tctgccgaag cgctgcgccc cggtaaaggt
    20701 gccggagaaa gtgacgcggg cggcggcagt tgacgacgct gttctgccca gaatgccgca
    20761 tcacgctgcc aggcttcgct ttcgcggtat tgctggtact cttccactac atcagcgaaa
    20821 ggcgtaaacg gcgaagcagg cgttggttcg ccacgcagcc atgtgcagta aatattggcg
    20881 atctggcgag taatggccgg gaaactgaag ccatcgacca gcaaatggtg ataacgctga
    20941 taccagtacc agcggttatc cgcaacatgt atcagctgat gaaagaccag tggtttaccg
    21001 ctatcgacgc gcaaatcttg ttgcaaatcc gccagcatta atgcctgcgc agtaccgtgc
    21061 ggatcaatat tggttcgcag gtcgataatt tctggctgtt cgaatatcag cgcatcatcg
    21121 acccactgcc agacttcgcc gttatcttcc gtaaaacgca tccgcaacgt atctgcttgc
    21181 gctagtccgg caaccaccgc gcgggccagt aatggcgcat caacatctcc agttaactca
    21241 acgtaatgcg ccacgctcca ggcggagggt aaatccgaca gtttttctgc catccagatg
    21301 ccgggctgtg cggcgaccaa aggtaaatgc tggctcattg tgcctcctgc aactgggtga
    21361 aattggccgg tgtcagagta cgccagtgga cttccagcca ctgctggcag gacgcctgtg
    21421 actgcggctg acacacaatg tcccagcctg ccgataagag gcattgttgc ggccacagac
    21481 tgaattgccc ctgcgcattg cgcaaaatgt aaaacgctcc ctgcggatca tcgaagggat
    21541 tactgaatgc catactcaac tcctgtcatg gaaaagtggt tgccagaggt cgattaaccc
    21601 ctgcatcaag ccaccgcgcc aacaaagcgc atcatgtccg ccgtcaacct gacgccagaa
    21661 aatggattct tttatagggt gcaattgcgc atacagcgcc tgattggccc gcatgatcat
    21721 cggctcgcga atacccgctt ccagcacaat gcgcagacct tcggcgctaa cttcaccagc
    21781 ttttagcttt tcaagtaaca cgccctcttg ctgcccgtcc cgatgcggcc accagtacga
    21841 tcctgactgg cttaatacac agccaaagcg ttcaggccag tgcagtccgg catacagcgc
    21901 ggacagccca ccaaaactct gcccggcgac cacggtgcga tcggcgcgat cgctaaaagg
    21961 ggcaatagct ttcaccaggg gtaataactc ttgctgtact gccagccaga aatccgcatt
    22021 acacggcagt tcgtgggcgc ggtgcgtggt gtcgatagcg tcgatcaaca catacacggc
    22081 gggaggaagt tgctgacgat gggtcagcga agtcagcaca ggccagacgg gcatactttg
    22141 cgcccaaaat tcgccatcga gcaaaactgc cagcggacgt tcttctgctg ttacatcgcc
    22201 ggtggtaaaa atccatacac gacgtgaatt tttcaaccgt tcacttttcc agataatttc
    22261 tttggctgct atttctggca cttgcggaca atcccatccc ggttgcagag gcgcttgcgg
    22321 catttcgagt gcagaaacag cgtgccctcg cccgcctttc cagctttgtg gattcagcgg
    22381 atcggctatt gcctggggta atagttttcg ccagccttca cgtaattcga ggcgatcggg
    22441 ggatggtgca gaaaaaatgt catcgcgttc ggtgggaata aagcagtagc tgccgcgcca
    22501 gttggcattg agttgtgtcg tccactgcca gacatcagta cctgcaattc gctgcatcga
    22561 ctggggctgg ctgttctgat gatgatcggt cacaccagtg atgtagatcc atacgcgctt
    22621 tatcgtcgag tattcttcag aaccttgggg atcacgccac cagaaagtga cctcaaacat
    22681 ttcgtcattc agacgctgcc attccgggcc atgtttcgac tgccaccagc tctcacttcc
    22741 tacttttaac gccgtcacac cataacccca tgtttactgt gcaatttttc attgattgca
    22801 gaaatatatt gataatatta ttgataacca tttgcatttg caatagcgta atggcgcgcc
    22861 gtgggaagcg cggacattta ttaaccaact gcactgcgtg tctttcagga tcaaaggttt
    22921 tcgcggtagc gggatgcgtc gtgttgagga cgaccatgcc cgacagttgc aattcgtggc
    22981 aaaaatgcag gaataaaaca atgaacaaga agattcattc cctggccttg ttggtcaatc
    23041 tggggattta tggggtagcg caggcacaag agccgaccga tactcctgtt tcacatgacg
    23101 ataccattgt cgttaccgcc gccgagcaga acttacaggc gcctggcgtt tcgaccatca
    23161 ccgcagatga aatccgcaaa aaccccgttg cccgcgatgt gtcggagatc atccgtacca
    23221 tgccaggcgt taacctgacc ggtaactcca ccagtggtca gcgtgggaat aaccgacaga
    23281 ttgatattcg cggtatgggt ccggaaaaca cgctgatttt gattgacggc aagccggtaa
    23341 gcagccgtaa ctcggtgcgt cagggctggc gtggcgagcg cgatacccgt ggtgatacct
    23401 cctgggtgcc acctgaaatg attgaacgta ttgaagttct gcgtggtccg gcagctgcgc
    23461 gttatggcaa cggcgcggcg ggcggcgtgg ttaacatcat taccaaaaaa ggcagcggtg
    23521 agtggcacgg ctcctgggac gcttatttca atgcaccaga acataaagag gaaggtgcca
    23581 ctaaacgcac caacttcagc ctgaccggtc cgctgggcga cgaattcagc ttccgcttgt
    23641 atggcaacct cgacaaaacc caggctgacg cgtgggatat caaccagggc catcagtccg
    23701 cgcgtgccgg aacgtatgcc acgacgttac cagccgggcg cgaaggggtg atcaacaaag
    23761 atattaatgg cgtggtgcgc tgggatttcg cgccattgca atcgctggaa ctggaagcag
    23821 gttacagccg ccagggcaac ctgtatgcgg gcgacaccca gaacaccaac tccgattcct
    23881 atacccgctc gaaatatggc gatgaaacca accgtctgta tcgccagaac tactcgctga
    23941 cctggaacgg cggctgggat aacggagtga ccaccagcaa ctgggtgcag tatgaacaca
    24001 cccgtaactc gcgtattccg gaaggtctgg caggcggtac cgaagggaaa tttaacgaaa
    24061 aagcgacaca ggatttcgtc gatatcgatc ttgatgacgt gatgctgcac agcgaagtta
    24121 acctgccgat tgatttcctc gttaaccaaa cgctgacgct gggtacggag tggaatcagc
    24181 aacggatgaa ggacttgagt tccaataccc aggcgctgac cggggcgaac accggcggcg
    24241 ctattgatgg tgtgagtgcc accgaccgta gcccgtattc aaaagcagaa attttctcgc
    24301 tgtttgccga aaacaacatg gagctgactg acagcaccat cgtaacgccg gggctgcgtt
    24361 tcgatcatca cagtattgtc ggcaataact ggagcccggc gctgaacata tcgcaaggtt
    24421 taggcgatga cttcacgctc aaaatgggca ttgcccgtgc ttataaagca ccgagcctgt
    24481 accagactaa cccgaactac attctctaca gtaaaggtca gggctgctat gccagcgcgg
    24541 gcggctgcta tcagcaaggt aacgatgacc tgaaagcaga aaccagcatc aacaaagaga
    24601 ttggtctgga gttcaaacgc gacggctggc tggcgggcgt cacctggttc cgtaacgatt
    24661 atcgcaataa gattgaagcg ggctatgtgg ctgtagggca aaacgcagtc ggcaccgatc
    24721 tctatcagtg ggataacgtg ccgaaagcag tggttgaagg tctggaagga tcgttaaacg
    24781 taccagttag cgaaatggtg atgtggacca ataacatcac ttatatgctg aagagtgaaa
    24841 acaaaaccac gggcgaccgt ttgtcgatca tcccggagta cacgttgaac tcaacgctga
    24901 gctggcaggc gcgggaagat ttgtcgatgc aaacgacctt cacctggtac ggtaagcagc
    24961 agccgaaaaa atacaactac aaaggtcagc cagcggttgg cccggaaacc aaagaaatca
    25021 gtccttacag cattgtgggt ctgagcgcga cctgggatgt gacgaagaat gtcagtctga
    25081 ccggcggcgt ggacaacctg ttcgacaaac gtttgtggcg tgcgggtaat gcccagacca
    25141 cgggcgatct ggcaggggcc aactatatcg cgggtgccgg ggcgtatacc tataacgagc
    25201 cgggacgtac gtggtacatg agcgtaaaca ctcacttctg atgctaacgt cagattgttg
    25261 acaaagtgcg cgtcgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaaaat
    25321 cttgccaatt caatatattg caggaccatg taggcctgat aagcgtagcg catcaggcag
    25381 ttttgcgttt gtcatcagtc tcgaatatgg tcgatatgaa aactacgcat acctccctcc
    25441 cctttgccgg acatacgctg cattttgttg agttcgatcc ggcgaatttt tgtgagcagg
    25501 atttactctg gctgccgcac tacgcacaat tgcaacacgc tggacgtaaa cgtaaaacag
    25561 agcatttagc cggacggatc gctgctgttt atgccttacg ggaatatggc tataaatgtg
    25621 tgcccgcaat cggcgagcta cgccaacctg tctggcctgc ggaggtatac ggcagtatta
    25681 gccactgtgg gactacggca ttagccgtgg tatctcgtca accgattggc attgatatag
    25741 aagaaatttt ttctgtacaa accgcaagag aattgacaga caatattatt acaccagcag
    25801 aacacgagcg actcgcggac tgtggtttag ccttttctct ggcgctgaca ctggcatttt
    25861 ccgccaaaga gagcgcattt aaggcatcgg agagacaaac tgaagcaggt tttctggatt
    25921 atcagataat tagctggaat aaacagcagg tcatcattca tcgtgagaat gagatgtttg
    25981 ctgtgcactg gcagataaaa gaaaaaatag tcataactct gtgccaacac gattaattga
    26041 caacatctgg tacgattcgc ccgcagccat cactgactgc gggcgaaagt gtaaagcagg
    26101 tgccttacca ctctgacctg acaaccggat atgcagggat tgctccccgc acaacggcta
    26161 cttcttcggt tcgtaagcga gaacagcctt aaactcaata ttacgttcct ttacggtgaa
    26221 ctcacacagc gagtctccga ccagaagcgt aaatcccagc accgtcaaac acagcactat
    26281 gactgccaca agggcatatt tcgtcagcat gtttatattg cctccatgcg aagaagggac
    26341 taccatccga gttgttgagg ttcggagtgg cagccccagg attgatagaa atatctcctg
    26401 gggcttcatc tttccagacc tcagagtaaa cctgaaaccg gaaagcttca ggcacccgcc
    26461 gttatcttac ccttcctcag caaaatatct gtttatttat acagtaagtc ctgtttcgga
    26521 cttctgttgg caacactcgt tattgctcgt aaaacgaaat cgcccgcagc catcactgac
    26581 cgcgggcgaa agtgtaaagc aggtgcccta ccatcctgac ctgacaaccg gatatgcggg
    26641 gattactccc cgcacaacgg ctacttcttc ggttcgtaag cgagaacagc cttaaactca
    26701 atattacgtt ccttcaccgt aaactcacac agcgagtctc cgaccagaag cgtaaatccc
    26761 agcaccgtca aacacagcac tatgactgcc gcaagggcat atttcgtcag catgtttata
    26821 ttgcctccat gcgaagaagg gactaccatc caaattgttc gggtttggag tggcagcccc
    26881 agggttgata gaaatatctc ctggggcttc atctttccag acctcagagt aaacctgaaa
    26941 ccggaaagct tcaggcaccc gccgttatct tacccttcct caacaaaatg gctgtttatt
    27001 tatacagtaa atcctgtttc ggacttctca ctcctcattt aatcaactga agctgagccc
    27061 gttttttctg catgcctcaa tcgtcaaaat tcttcgccat cagccagctc tgccataact
    27121 tgccacattg aaaaagtagc caaattgctt cagtcaccat catcaaaaaa agaaacattc
    27181 cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa aacgccaaca
    27241 taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac cctcggtatt
    27301 gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga ctcttcaatg
    27361 ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga tatcaccgaa
    27421 agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc cgggcaattt
    27481 tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga aatttgcggc
    27541 ggtggcacac acccgtttca gaaatggcag cgtcaggagg tatgcgataa cgagcgctat
    27601 caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt tggtcagcat
    27661 gtccatgttg gctgcgccag tggcgatgac gccatttatt tgctgcacgg cttgtcacgg
    27721 tttgtgccgc actttatcgc cctttccgcc gcgtcgccat atatgcaagg aacggatacg
    27781 cgttttgcct cctcacgacc gaatattttt tccgcctttc ccgataatgg cccgatgccg
    27841 tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtttgagtta caccacgatg
    27901 atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt tggcacggtg
    27961 gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat ggcgggatta
    28021 attcaggcta ccgcccactg gttactgaca gaacgcccgt tcaaacatca ggagaaagat
    28081 tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atgggctgga aggcgtcatt
    28141 accgatccgc acactggcga tcgtcgaccg ctaacggaag acaccttgcg attgctggaa
    28201 aaaatcgccc cttccgcaca taaaattggt gcatcgagcg cgattgaggc cctgcatcgc
    28261 caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc cgatggtggc
    28321 tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccgatgacta agtagggaat
    28381 tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac aatgttatga
    28441 aacacccttt agaaaccttg actaccgcag caggcatttt gctgatggct ttcctctctt
    28501 gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg gtgaccatgt
    28561 tccatctgat ggatcttagc tagctttaca ctttattgtt ttgtctgtgg tttttagtgc
    28621 tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc tcgctggcgg
    28681 attaaacgtc gattatcgcc gtacttcgcg gcatactttg cttattctcc ttcgcctaaa
    28741 ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctggc ccttgagtta
    28801 ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa aattggcggc
    28861 aaaattttta tgctgtcgtc ggagctgcgc ggcgtcccct ttatcaatct gaagtccgat
    28921 ccacaaaaat ccctgttaaa tcagcagata tacccgagca ttaagccagg gtatcacatg
    28981 aataaaaagc actggatttc ggtgtatccc ggcgaggaaa tctccgaagc gttacttcgc
    29041 gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg cgatcaaaaa
    29101 agagtgcgtc caggctaaag cggaaatcta tagcgcattt ttctcgctta ccatttctcg
    29161 ttgaaccttg taatctgctg gcacgcaaaa ttactttcac atggagtctt tatggatatc
    29221 atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagtaa aaaacttacc
    29281 ccggaacagg ccgagcagat caaaactctc ctgcaataca gcccatccag caccaactcc
    29341 cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt tgccaaatcc
    29401 gctgccggta attatgtgtt caacgaacgt aaaatacttg atgcctcgca cgtcgtggtg
    29461 ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga ccaggaagat
    29521 gctgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg tcgcaagttc
    29581 ttcgctgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc aaaacaggtt
    29641 tatctcaacg tcggtaactt cctgctcggc gtggcggctc tgggtctgga cgcggtaccc
    29701 atcgaaggtt ttgacgccgc catcctcgat gcagaatttg gtctgaaaga gaaaggctac
    29761 accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa cgctacgctg
    29821 ccgaaatctc gtctgccgca aaacatcacc ttaaccgaag tgtaattctc tcttgccggg
    29881 catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt ttcagccgtc
    29941 atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc caggttgaag
    30001 atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc ggtattattt
    30061 ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg accttcgaaa
    30121 aacgagccat cgccagttca cggctttggt tgcaaatcat aacccagaat aaactcttcc
    30181 accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta ttctggctgc
    30241 aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg atcatgatgt
    30301 atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg cagaaattcc
    30361 cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg atcggcgcga
    30421 tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg attctgatca
    30481 gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg attcttggtc
    30541 tggtggccgg tattcagctt tccgcgaacg atatgctgaa actgggcgac tggctggaga
    30601 tgccgaaata cggcgcagat ggcgcggtga tcgatattgg gttaaccacc gtcaaagtgc
    30661 gtaactggga caataccatt accactattc ccacctggtc tctggtttct gactccttta
    30721 aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt atcagtattg
    30781 atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat aaagcgcatt
    30841 tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat cgccagcaag
    30901 gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc tttcgtgcct
    30961 atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc ttaatggtac
    31021 gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt actaacaccg
    31081 tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata tttgcgattg
    31141 tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt cgctctctgg
    31201 cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga gagcggacat
    31261 cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa atgccacaaa
    31321 caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat ccatcgtgca
    31381 catcagcagc aaaatcatgc cgaggaaggc aatgcagaga tagtttccga acggatagag
    31441 cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gctgcacgaa aacgcagatg
    31501 cgccagacag atcataatcc agttcaacag cagcgttgct accaccagcg ccatcagcag
    31561 accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag tgatcgctcc
    31621 ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca aaaacttcgg
    31681 cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa ccccgctgtt
    31741 atacactgac agcgatgcta ccagaatgac gaagttcagc gcagaagcta ccacgttgct
    31801 gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt ccacccacgg
    31861 atagagcgcc agtaaaacca ccagtgaacc gatgtaaaac agcaggatgc gatacaccac
    31921 ctgatttacc gcttttggaa tgcttttatc cggatcgcgc gcttcagcgg cagtaatccc
    31981 aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa tcagcccatt
    32041 ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg ctttctcgcc
    32101 gccgtgacca gaaaacagca gccacaggcc aaagccgatc ataccgatga ttgccagcac
    32161 tttaatcaac gcaaaccaga actcggtttc gccatataag cgcacgttca ccaggttaac
    32221 ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat ccgggaacca
    32281 gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca cgaacattac
    32341 ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat aggcaaagtg
    32401 ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc gcataatcag
    32461 gaaagcgatg atcccggcga cgccgtaacc cagcaataca gccggacccg ccatctgaat
    32521 cgccggacca atgccaagaa acagaccagt accaattgcg ccacccagcg caatcagttg
    32581 aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg cagtatcttc
    32641 cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt tgaggggcac
    32701 cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa cggggtatcc
    32761 tgcttttatt tccgtacccg atgtcggtgc agccacatca gcttatacgc cgccgggata
    32821 ataaacagcg acagcaaagg tgcggtgatc atgccgccaa tcatcggtgc ggcaatccgg
    32881 ctcatcacct ctgaaccagc ccccgttccc cacagaatcg gcagcagacc ggcgataatc
    32941 accgccaccg tcatcgcttt cgggcgcacg cgcagtaccg cgccgtgata caacgcctca
    33001 tccagcttct gctcactgaa tgtttgcgga ttattcaacg acggctcggc ctctatggcg
    33061 tgacgtaaat acatcagcat caccacgcca aattcggcgg cgaccccggc aagggcaata
    33121 aaaccggtgc ccgttgccac agaaagatga aagcccatcc accacagcaa ccagatgccg
    33181 cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgcccac ccgacggaat
    33241 gccagataca acagcacgaa gatgatcatc agtgtcatcg gcaccatgag tttcagctta
    33301 tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt gcccggtttt
    33361 aactgcactt tttcagctat cgctttttgc aaatcgtgaa ccaccgacac catgtcacga
    33421 tcgcgggcat caatataaat ccagctcgtc gggcgcgcat tctcggtttt cagcatcgac
    33481 ggtccggtag agactttaac gtcggccacg tctgccaggg tgatttgctg cttcatcggc
    33541 gtcaggatcg gtagctggcg cagtgcctgc gggctatcgc gccagctttg cggataacgc
    33601 agattaatgg gataacgggc aatcccttcc accgtttcgc caaccatcgc cccgcccacc
    33661 gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgcgc ggctttttca
    33721 cggttaatct caacgttgat atagcgccca ccttccagac gctcggctag agcagaagcc
    33781 acgcttggca ccgttcgcgc tacttcttca atttgctcag ccatcgcgtc gatatccgcc
    33841 agcacagtgc cggaaacttt aatgccgatg gggcttttaa tgccggttga gagcatatcg
    33901 atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagccg cacggtgtta
    33961 tccagttcct caatgatttt gtccatcgtc atgccaggcc gccactgatc ctgcggctta
    34021 agctggatgg tcgtttcaac catctcgagc ggagcagaat cggtggcggt ttccgctttc
    34081 ccggttttgc caaatacccg cgccacttca ggtacgctca taattagctt gtcggttttt
    34141 tgcagcatac tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg catatacaac
    34201 aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag cggccagaga
    34261 accgtcagca ccgaaagcgc tgccaccaac agcgtggttt tcggccagtg cagtactttc
    34321 agcaatagag gatgataaac acgaatcaaa aagcgattga gcgggttact gctttccggc
    34381 ggaattttgc cacggatcca gtaccccatc aggataggga tcactacgat cgccagcagc
    34441 gccgcgcccg ccatcgcata ggttttagtg aacgccaacg ggccaaacag acgcccttcc
    34501 tgcccttcca gggtgaagat cgggataaac gacaacgtga taatcagcag actgataaat
    34561 agcgccggac ccacttcaac agatgcatcg gtgatcacct gccagcgcgt tttattatcc
    34621 agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgtgc attttcgatc
    34681 atgacgatag cggcatcgac catcgccccg acggcaatcg caatgccgcc caacgacata
    34741 atattggcat tcagtccctg gaagtgcatg acaataaaag caatacacaa ccccagcggc
    34801 aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc acagactacc
    34861 gccaccacga taaactcttc cagcaacttg ccgctgaggt tgtcgatggc gcggtcaatg
    34921 agctggctgc gatcgtatgt tgtaactatc tccacgcctt ccggcagact acttttcagc
    34981 gtttccagtt tgtccttcac ggaggcgatc acttctcggg cgtttttgcc ggagcgcagg
    35041 atcaccaccc cgcccgccac ttcgccttcg ccattcagtt cggcaatgcc ccggcgcatc
    35101 tccgggccaa tctggacctt cgcaacatca cgcagataaa cgggcacgcc attttctctg
    35161 gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgca gatagccgct ggcgcgcacc
    35221 atatattccg cttccgccag ttcgatcgac gaaccgcccg cttcctggtt tgaagcatcc
    35281 agcgcgcttt ttacttcggc tagactgatg ccatattgcg ccaggcgctg gggatcgata
    35341 accacctgat actctttcac cacgccgccc accgacgcca cttccgcaac gtcagggatg
    35401 gtttttagct catatttgag aaaccagtcc tgcaatgagc gtaaatcggc cagatcgtgc
    35461 ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt ggcatctggt
    35521 cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag gtactccagc
    35581 acccgcgaac gcgcccagta cggatcggta ccatcttcga aaatgacata cacataagag
    35641 tctccaaact gcgagaaacc gcgcacagtc ttcgcgccag gcaccgacaa catggtggtg
    35701 gttagcggat aagtcacctg attttcaacg atttgcggtg cctgaccggg atagctggtt
    35761 ttaataatca cctgcacatc ggagagatcc ggcagcgcat ccactggcgt gttaatgatg
    35821 gtccaggtgc cccagatgct cagaaacaac gcgcccatca gcaccaaaaa acggttcgcc
    35881 accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca ttggtagcac
    35941 tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca atcaggaaca
    36001 ggccgctgga aaccaccttt tcaccttccg ccagaccaga gtgtaacgcg gtgacgcctt
    36061 gcgatgcctg gaaaacggca acgcgtttcg gtacaaagcg tccgtcggca tcaacggtaa
    36121 tcacccgctg ttcgttgccg gtatcaatca gcgcctgtga cggaatgagc agcatcggtt
    36181 cgctggcggt gttgagttgc aaccaggcgt tcatgcccgg ttttagcgcc tcgtcggcgt
    36241 tgtcgacttc cagacgcagc tgcagcgtgc gggtcgcggc atccacgcca ggtagcagcg
    36301 tccatttgcg gatggtgagt gttttatccg gtcgcgccgg aacggtgagc gtaaactgcg
    36361 aggcatcttt caccagccag gcgatagact ccgggatcgc agcagtgacc cacaccgggt
    36421 ccataccctg aattttcgct accacgttat ctttggcgat attcattccc gcgcgcagat
    36481 caaacgcggt gatcacgcca tcaatgggtg ctttgagcgt aaagcgagtc tggatttttt
    36541 gcgtggcgat caggcggcga atatccgcct ccggcattcc cgccagtcgc agccgctcaa
    36601 gaatgccttc agtctgggtc gccgtaccgc cggtttcgcg cagcagtaaa tactcactct
    36661 gcgcttccac ccagtcagga atggtcaggt cgagaagcgg tgtgcccttt tgtactttat
    36721 cgcccacggt aagcggatac accttgtcga taaacccggc agcgcgagcc tgcacaatgg
    36781 catactgata ctcgttgtaa ctgacattcg ccgggaaact ctgggcaaaa gtcagcggtc
    36841 cgcgcgtgac ggtagccgtt ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca
    36901 caccagacgc agaactctct tcatcggcat atttcggcac cagatccata tccataaacg
    36961 gcgatttacc tggtttatcg aaccgcgtat tggggtacat cgggtcgtac cagaataaga
    37021 ttttacgttc tgcggtcgac gttttttctg cgggtggttc agcctttgca aaccaggtaa
    37081 aacctgccgc agaaataata ccgcccgcga tcatgctgcc gataataagc gcgatttttt
    37141 tcatctcatt aaacctgggt tactggctga ctttaatatc ctgtaataaa gaaaggttgc
    37201 cctgctggac aaaattaaac gccactttgt cgccggtttt aatttcactc attttcgtct
    37261 gcggggtgat ggtaaagcgc atagtcatct ccggccagtt cacggcagca atcggatcgt
    37321 gatggatggt gatttttttg ctttccagat caataccctt taccacgcca gtggcgctaa
    37381 taacctgcgg ttgtacttcg ctcatggttt catgatgatg ttcgttagcc tgggcattaa
    37441 agccaataac ggtaaacaga ctgaacattg cgacttgcag tgcttttttc atttattctc
    37501 tcctggagtt aaaaattatt gctgccaacc gccccctaag gcggtataca aagaaatttc
    37561 gttaacctga cgggcataat tcaggtcgag taaagtttgt cgggttgcaa ataaagaacg
    37621 ctcggcatcc agcacttcca gataacttac cgcgccgtgc tgatataatg cccgcgcccg
    37681 ttgcaaagta atttgcagcg acgccagata acgctgctgg gcgctgattt gatcgttcag
    37741 gctttgacgt aatgccagcg catctgccac ttctttaaag gcgttctgga ttttctgttc
    37801 ataattcact accgactgct gctggcgaat ttcggcgata tccagattgg cctggttgcg
    37861 tccggcatta aaaatgggga tctcaatttt aggaataaaa ttccacatcc cgctgctagc
    37921 gttaaataat gaagacagat cgctgctggc ggttgatatt ccgctggtca ggctgataga
    37981 cgggaaaaat gccgcacgcg cagcaccaat attagcatta gccgccatta acgcgtgttc
    38041 agcttccata atatccgggc gctgcaataa gatttgcgac gacaagcccg ccggtaattt
    38101 aacgctttgc aggctgccgc tgtttactgt ctgcgcttgc ggcagtttgc cgtagcttcc
    38161 caataacagt tgcaatgcat tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc
    38221 actgcgggta ctttctatca ccccgcgagc ctgttccagc gccagaacat tgctgctacc
    38281 ggtcaacagt tgtttttcga caaacgcata tgactgctga taattacgca gcgtttcttc
    38341 ggctatttgc aattgcgcat acgccagttg ctgattgaaa tagctttgcg cgacattaga
    38401 aaccagcaga atatgcaccg cgcgctgagc ttcctcagtg gctaaataat tttgtcgctc
    38461 ggcttcgctc atgttcttta agcgaccgaa aaaatcgagg tcaaagctgg cgttaaggcc
    38521 ggtcgaaaac tcccgtgtcg tggcggtatt ccctttaaga ttaccgctcc agctgccgct
    38581 gccctcgcca ttgagctgtg ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc
    38641 ctcctgcact ttcagcgtcg ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat
    38701 cagcgtcttc acctgattat caacaaaaaa ggtgcgccag cccacgttct ggtagttatc
    38761 tgctgcgtta accaagccgt tctggctgag tgagaactgc tgcggcacgg gcattgccgt
    38821 acgctgataa tccggtgcca gtgaacaacc ggttagcgca agggccacac aaaatggcag
    38881 aagtttacaa ggagacatag gctcataatt tctggtgatt ttataccgcc aactttactc
    38941 gccaggctct gattttccgg tgacaggaaa atgacaaaat tgtcattttg ccaataagcg
    39001 attgccatct gatcccgcta ctctagaatt gcccgggcaa catgcggagg aaaaatgaaa
    39061 ctgttgattg tcgaagatga aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa
    39121 gccggttttg tggtcgattt ggccgacaac gggctgaatg gctaccatct ggcgatgacc
    39181 ggtgattatg acctgataat cctcgatatt atgctgccgg acgtgaacgg ctgggatatc
    39241 gtgcgcatgt tgcgctccgc caataaaggg atgccgattc tgttgcttac cgcgcttggc
    39301 accattgaac atcgcgtcaa ggggctggag ttgggggccg atgactatct ggtgaagccg
    39361 ttcgcttttg ctgaactgct ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg
    39421 attatcgaaa gtcagtttca ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc
    39481 acccgcagcg gcacgcgcat cactctgacc agtaaagagt ttactctgct ggagtttttt
    39541 ctccgccatc agggcgaagt gctgccccgc tcgcttatcg cctcgcaggt gtgggacatg
    39601 aatttcgaca gcgacactaa cgccattgat gtggcggtga agcggctacg cggcaaaatc
    39661 gacaacgact ttgagccgaa gctgattcag accgtgcgcg gcgtgggcta tatgcttgag
    39721 gtgccggatg gtcagtaagc catttcagcg cccgttttcg ctggcaaccc gtctgacctt
    39781 ttttatcagc ctggccacca tcgcggcgtt tttcgccttt gcatggatca tgatccactc
    39841 agtgaaagtg cattttgccg agcaggatat taatgattta aaagaaatta gcgccaccct
    39901 tgaacgggta cttaatcatc ctgacgagac gcaagcccga cgcttaatga cgctggaaga
    39961 tatcgtcagt ggttattcca acgtgttgat ttccctggca gatagccacg gcaaaacggt
    40021 gtatcactcc cccggtgcgc cggatatccg cgagtttacg cgtgacgcca tacccgataa
    40081 agacgctcag ggtggcgagg tgtatctcct ttccggcccg acgattatga tgccaggcca
    40141 cggtcacggg catatggaac acagcaactg gcggatgatt aacttgccgg ttggcccgtt
    40201 ggtggacggc aaaccgattt atacgctcta catcgcgctt tcgatcgatt ttcatcttca
    40261 ttacataaat gatttgatga ataaacttat tatgaccgca tctgtcatca gcatcctgat
    40321 cgtctttatc gtgctattgg cggtacataa aggtcacgcg ccgatccgca gcgtcagccg
    40381 tcaaatccag aatatcacct cgaaagatct cgacgttcgc cttgacccgc agaccgagcc
    40441 cattgagctg gaacagctgg tactgtcgtt caaccatatg atcgagcgta ttgaggatgt
    40501 ctttacccgc cagtccaatt tctcagcgga tatcgctcac gaaattcgca cgccgattac
    40561 caatctcatt acgcaaacgg aaatcgccct cagccagtct cgcagccaga aggagctgga
    40621 agatgtgctc tactctaatc tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat
    40681 gctgtttctc gctcaggccg ataacaacca gctaatcccc gaaaagaaaa tgctcaacct
    40741 ggcggatgaa gtcagcaaag tgttcgattt tttcgaggcg ttagcggaag atcgcggcgt
    40801 ggagttgcga tttgttggcg acaaatgtca ggttgcgggc gatccgctga tgctgcgtcg
    40861 ggcgttaagc aacctgcttt ctaatgccct gcgttatacg ccaaccagag agacaattgt
    40921 agtgcgctgt cagacggtcg atcaccaggt gcaagttacc gtcgaaaacc ccggtacgcc
    40981 cattgcgccc gagcacttac cgcgattgtt tgaccgtttc taccgcgttg acccttcccg
    41041 ccagcgaaaa ggcgaaggta gcggcattgg gctggcgata gtgaaatcga ttgttgtcgc
    41101 gcataaaggc acggttgcag taacgtcaga tgcgcggggg acaaggtttg tgattatgct
    41161 gccggagaga gagtgattat taagttcaaa ttcagggcgt ggtgcatcac ctcctggtat
    41221 tgttgatcaa caaattagag aaaaacttat taaacatatg aaatatcgaa ggcttttatt
    41281 tttcaaagtt tcacgagatg tttgtatatg aaacttagtt aaatctgtgt ttcttccaat
    41341 tgtagatcac aatgcccttt ctaaattttc atcgttataa acaagccatc tatcatataa
    41401 agcaatgaat aatttaatcg cgattaatca tcattaattg ttatattatt cactcaatct
    41461 atttgtcaag tgaatttgat aaattcctaa tttaaaatat ccactattga gccagtattt
    41521 aacaacgtta agatgtgcaa tgtgcagatc atgttatcag ggtggagcgg tagcatatat
    41581 ttcttatgca atcattcatt ttaataatgt taattggatt atttattgct gtgaataaaa
    41641 atatattatg aatattggtt taactttgcg tttttgttac tcgttgtgat atgcgtacct
    41701 gtcctgaata acgccaagaa acaagaggtt gagcttgtga ttattttttt gactgaacag
    41761 acacccgttt ctgtaaagta atatttaaac aaaattctca cataaccata gcattttctg
    41821 ttccttccct aataacgtct gaagtcgtcc cgcttatcac attatccgca gttgtacggt
    41881 acggatcatt attcatttta ctggaggttg ctatgtcaac cggattacgt ttcacactgg
    41941 aagtggacgg cctgccgccg gacgcttttg cggtggtttc ctttcatctg aaccagtcac
    42001 tctcttcgct cttttccctc gatctctccc tggtcagcca gcagtttctc tcccttgaat
    42061 ttgcgcaggt gctggacaaa atggcctacc tgacgatatg gcagggcgat gaagtacagc
    42121 gccgggtgaa aggcgtggtg acctggtttg aactcgggga gaacgacaaa aaccagatgc
    42181 tgtacagcat gaaggtgcac ccgccgctgt ggcgtgccgg tctgcgccag aacttccgta
    42241 tcttccagaa cgaggacatc aaaagcatcc tcggcacgat gttgcaggaa aacggggtga
    42301 ccgaatggag tccgctgttc agcgagccgc atccttcccg tgagttttgt gtccagtacg
    42361 gtgagactga ttacgatttc ctgtgccgga tggcggcgga ggaaggcatc ttcttttatg
    42421 aggagcatgc ttacaaaagt accgaccaga gcctggtgct gtgcgacaca gtccgccatc
    42481 tgcccgaatc ttttgaaatc ccatggaacc cgaacacccg taccgaggtg agcaccctct
    42541 gcatcagcca gttccgctac agcgcacaaa tccgcccttc ttccgtggtg accaaagact
    42601 acacctttaa acgccccggc tgggccggac gttttgaaca ggaaggccag caccaggact
    42661 accagcgcac gcagtatgaa gtgtatgact accccggacg tttcaagggt gctcacgggc
    42721 agaactttgc ccgctggcag atggacggct ggcgaaacaa tgcagaaacc gcgcggggaa
    42781 tgagtcgctc gccggagata tggccgggac gacgaattgt gctgacgggg catccgcagg
    42841 cgaacctgaa ccgggaatgg caggtggtgg caagtgaact gcacggcgaa cagccacagg
    42901 cggtgccagg acggcaggga gcggggacgg cgctggagaa ccattttgcg gtgatcccgg
    42961 cagacagaac atggcgacca cagccgttgc tgaaaccgct ggtcgacggc ccgcagagcg
    43021 ctgtcgtgac aggaccggca ggcgaggaaa tcttctgcga cgaacatggt cgcgtgcggg
    43081 tgaagttcaa ctgggaccgt tataacccgg cagaccaaga cagttcgtgc tggatccgtg
    43141 tggcacaggc gtgggcaggc accggttttg gccacctggc gataccgcgt gtgggtcagg
    43201 aagtgattgt agacttcctc aacggcgatc cggaccagcc gatcattatg gggcgcacct
    43261 accaccagga aaaccgcccc cccggcagcc tgccgggaac aaagacgcag atgaccatcc
    43321 gctccaaaac gtacatgggc agcggattta atgagctgaa gtttgatgat gcgacaggga
    43381 gagaacaggt ctacatccac gcgcagaaga acatggatac cgaagtgctc aacgaccgta
    43441 ccaccaccgt aaaacacgat caccgcgaaa ccgtaaaaaa tgaccagacg gtcacgatcc
    43501 aggaaggtaa ccgccttctt acggtggaaa aaggccacaa gatcaccgga gtactgaaag
    43561 ggtctttatc tgaggatgtc tttcaggaca gaggcacgat tgccggttcg gtgcatgttg
    43621 acgctgtaaa caatggtggc gaaggcgacg gtatacaggc ttatacggcg attaaggaaa
    43681 ttttgctggc cgtggaggaa agcaaaattg cgctgacgcc ggatagcatt cagctacagg
    43741 tcggggaatc gacggtaatc aggctgtcga aggatggcat caccatcgtg ggcggttctg
    43801 ttttcatcaa ctgattcact cagattatca gggagtattt tatgcagttc acttttaatg
    43861 aaggccacat tcaactgccg tctcaatggc aggatcagtc aatgcaggtc ctggtgtcca
    43921 cagataacag tggcatcaat ctggtcatta cccgcgaagc cgttccacag ggaaccctga
    43981 caccagagct gtaccaggaa acgctggcgc tgtaccaggg caaacttgat gggtacaccg
    44041 aacatgcctg ccgggaaatc actctggcag aggccccggc ctggctgctg gattattcct
    44101 ggaacggtcc ggaagatgag gggaatcagg ggcggattag ccagatagcc gtattccagc
    44161 ggcgtggaga tacgctgctg accttcactt tcagcacctc cttatccctg aagaacagtc
    44221 aaaagacgat gctgctggag gtgataaaga gcttcacccc gttgccaccg gagaatgaca
    44281 tccaaaagga ccagccgcgc tgacataaac gagggataac acatgagtga aggaccaggc
    44341 gggccacagg gagcgaccgc aggcggtacg ctggcaatgc gaatgctgtc acagcaggcg
    44401 atggtcgcca gccagatgaa acgggcagcc aacgacaaag ccattgcaca gatgttggca
    44461 tcaaaaaagt ctggcccccc cgccgccagg ctgggcgatg aaattcagca caagagtttt
    44521 ttgggggcgc tggcaggggc cgtactgggg gcgatagtga ccatcgcaga aggttgcctg
    44581 attatggccg cctgtgccac cggcccttat gcgctggttc tggtacctgc gctgatgtat
    44641 gccagctata aggcgagtga ttatgtggag gagaaacaga accagcttga atcatggata
    44701 aacagctttt gtgacacgga cggcgccatc aataccggtt ctgaaaacgt aaaaattaac
    44761 ggcgagctgg ccgcacgtgc agcggtcacc cttccccctc ctcccccacc tggagcaata
    44821 cctgaagtcc cacaggggga accctcatgg ggtgatattg ccactgacct gcttgaatcg
    44881 gcagcggaaa aagcagtacc actggcgaag gcctggggga acgctgttat caccctgacg
    44941 gacagcaatg ccggttttat ggatcgcgtc agcgccggcg catcgcttct gtttcccgcc
    45001 ggtccggtat taatggagtt tgccaccatg gtgggcgggc gtggcgaaat caaaaaagat
    45061 gtggatttcc cggaagccgg tgaggacacg gcgctctgcg acaaggagaa caaaccaccg
    45121 aggatagccc agggcagcag caacgtcttt atcaacaatc agcctgccgc gcgcaagggc
    45181 gacaaactgg agtgcagtgc ggcgatcgtg ggaggttcgc cggacgtctt tattggcggt
    45241 gagcaggtca catatctgga tatccagccg gagttcccgc cgtggcagag aatgatcctg
    45301 gggggaataa cgatagccag ctatcttctg ccgccagcgg gactactggg aaaactgaag
    45361 aatctggcga gactcggcaa actgggaaac tgctggggaa aagcgggaag ctgctgggcg
    45421 caaagctcgg cgcgttgctg gggaaaacag gtaagtcgtt aaaaagtatt gccaataaag
    45481 tcatcagatg ggtaacagat cctgtcgatc cggtaaccgg cgcatactgc gacgaacgta
    45541 ccgacttcac cctgggccag accctccccc tctccttcac ccgtttccac agttctgtac
    45601 tgccgctgca tggcctgacc ggcgtgggct ggagcgactc ctggagcgaa tacgcctggg
    45661 tgcgtgaaca gggaaaccgg gtggatatca tcagccaggg agccacgctg agatttgcct
    45721 tcgacggtga cagtgatacg gcggttaacc cgtatcacgc ccagtacatt ctgcgccgcc
    45781 gcgatgatta tctggagctg ttcgacaggg atgcactgag cagccgcttc ttttatgacg
    45841 cctttccggg aatgcgtctg cgccacccgg tgactgacga taccagcgat gaccgcctgg
    45901 cacacagccc caatgaccgg atgtacatgc tgggcgggat gagcgacacc gccagcaacc
    45961 gcatcacgtt tgagcgcgac agccagtacc ggatcacggg tgtcagtcac accgacggga
    46021 tccggcttaa actgacgtac cacgccagcg gctacctgaa agccattcac cgcacggata
    46081 acgacataca gacgctggcg acctacgaac aggatgcgcg ggggcggctg acagaagcgg
    46141 atgcgcggct ggactaccac ctgttttatg agtacgacgc tgcggaccgg atcatccgct
    46201 ggtccgataa cgaccagacg tggagccgtt tcacctacga tgaacagggc cggtgcgtga
    46261 atgtcaccgg ggcggagggc tattacaacg ccacgctgga ctatggtgac ggctgcacca
    46321 ccgtgacgga cggcaagggc attcaccgtt attactatga tcctgacggc aatattctgc
    46381 gggaagaagc gccggacggc agcaccacca tgtatgaatg ggatgaattc catcacctgc
    46441 tggcccgcca ctcccctgcc gggcgggtgg agaaatttga atacaacgcc gcacacggtc
    46501 agttaagccg ttacacggcg gcagacggcg cggagtggca gtaccgctat gatgagcgcg
    46561 gcctgctcag caacatcacc gaccctgccg ggcagacgtg gacacagcag tgtgatgaac
    46621 gcggcctgcc ggtaagtctg gtgtcgccac agggcgaaga gacccggctg gcatacaccg
    46681 ctcaggggct gctgtcggga atattccgcc aggatgaacg gcgtctgggc atagagtacg
    46741 accaccacaa ccggccggaa acactcaccg acgtgatggg ccgtgaacac cacaccgaat
    46801 acagcggtca cgacctgcca gtgaagatgc gcggccccgg cggtcagtca gtgcggttac
    46861 agtggcagca gcaccataaa ctgagcggca ttgaacgtgc tggaaccggc gcggaaggat
    46921 ttcgctatga ccgccacggc aacctgctgg cctggacgga cggcaacggt gttgtctgga
    46981 cgatggaata cggtccgttc gattttccgg tggcgcgaac ggacggtgaa ggccaccgct
    47041 ggcagtaccg ctacgataaa gacacgctgc aactgacaga agtcatcaac ccgcagggtg
    47101 agtcatatct ttatgttctg gataactgcg gccgggtgac ggaagagcgt gactggggcg
    47161 gcgtggtctg gcgttaccgc tatgacgccg atggcctctg taccgccaag gttaacggcc
    47221 tggaggaaac catcctctac agccgggacg ccgcaggccg cctggcagaa atcatctcgc
    47281 cggaaggcaa aacgcagtat gcctatgaca aatccggcag gctgacgggt atcttcagcc
    47341 cggacggcac atcacagcgc accggctatg acgaacgcgg gcgggtgaat gtcaccactc
    47401 agggccgacg ggccattgaa tacaactacc ccgatgaaca caccgtcatc cgctgtatcc
    47461 tgccaccgga agatgaacgc gacagacacc ccggtgaatc cctgctgaaa accacgtacc
    47521 gctacaacgc cgccggagaa ctgacagagg tcattctgcc gggggatgag acgctgacgt
    47581 tcagccgtga tgaggcggga cgtgaagtgc tccggcacag taaccggggt tttgcctgtg
    47641 aacagggctg gaatgcagcc ggtcagcctg tcagtcagcg cgccggattt tttccggcag
    47701 aagccacatg ggacgggctg gttccctcac tggtacggga gtaccgttac gacagcgcgg
    47761 gtaacgtgtc aggcgtcacc agccgggaag attacggacg ggaaacacgg cgggagtatc
    47821 ggctggaccg gaacggccag gtcacggcgg tgacagcctc aggcaccggg ctgggctatg
    47881 gcgaaggcga tgagtcctat ggctatgaca gctgcggcta cctgaaggcg cagtctgcgg
    47941 gcaggcaccg gataagtgaa gagactgacc agtatgccgg aggccaccgg ctgaaacagg
    48001 ccggaaacac gcagtatgac tatgacgccg caggccggat ggtcagccgc accagacacc
    48061 gtgacggcta ccgaccggaa acagagcggt tccggtggga cagccgggac cagctgaccg
    48121 ggtattgcag cgcacagggt gagctgtggg aataccgcca cgacgccagc ggcagacgaa
    48181 cagagaaacg ctgcgaccgg aagaaaatcc gcttcacgta tctgtgggac ggcgacagta
    48241 ttgcggaaat ccgggaatac cgcgatgata aactgtacag cgtaaggcac ctggtgttta
    48301 acggctttga gctgataagc cagcagttca gccgggtacg gcaggcgcat ccgtcggtgg
    48361 ccccgcagtg ggtgacgcgg acgaatcatg cggtgagcga cctgacgggc cgcccgctga
    48421 tgctctttaa cagtgaagga aagacggtct ggcgaccggg gcagaccagc ctgtgggggc
    48481 tggcactcag cctgcccgca gacaccggct acccggaccc gcgcggggaa ctggacccgg
    48541 aagccgaccc cggcctgctg tatgcaggac agtggcagga tgcggaatcc gggctgtgct
    48601 ataatcggtt ccggtattac gagccggaaa ccggaatgta cctggtgagt gatccgctgg
    48661 ggttgcaggg cggggagcag acttaccggt atgtgccgaa tccttgtggg tgggttgatc
    48721 cgctgggatt ggctgcaagt tctaaaatca gcagtttgat ggactatatt ggcgatggtc
    48781 gtcgtgttag tgggcatacg ggtttcctgg atggggttcg tttatcacgt agtcaaataa
//
LOCUS       DAETQN010000015        46686 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.015, whole genome shotgun sequence.
ACCESSION   DAETQN010000015 DAETQN010000000
VERSION     DAETQN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.015"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="K0W68_000923"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="K0W68_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBI3072570.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="K0W68_000924"
     CDS             complement(280..873)
                     /locus_tag="K0W68_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBI3072571.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="K0W68_000925"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="K0W68_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI3072572.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="K0W68_000926"
     CDS             1788..2183
                     /locus_tag="K0W68_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBI3072573.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="K0W68_000927"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="K0W68_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBI3072574.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="K0W68_000928"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="K0W68_000928"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBI3072575.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="K0W68_000929"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="K0W68_000929"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBI3072576.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="K0W68_000930"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="K0W68_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBI3072577.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="K0W68_000931"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="K0W68_000931"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBI3072578.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="K0W68_000932"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="K0W68_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBI3072579.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="K0W68_000933"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="K0W68_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBI3072580.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="K0W68_000934"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="K0W68_000934"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBI3072581.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="K0W68_000935"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="K0W68_000935"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBI3072582.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="K0W68_000936"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="K0W68_000936"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBI3072583.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="K0W68_000937"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="K0W68_000937"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBI3072584.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="K0W68_000938"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="K0W68_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBI3072585.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="K0W68_000939"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="K0W68_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBI3072586.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /locus_tag="K0W68_000940"
     CDS             17158..17709
                     /locus_tag="K0W68_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBI3072587.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="K0W68_000941"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="K0W68_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBI3072588.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="K0W68_000942"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="K0W68_000942"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBI3072589.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="K0W68_000943"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="K0W68_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HBI3072590.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /locus_tag="K0W68_000944"
     CDS             complement(21354..22376)
                     /locus_tag="K0W68_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3072591.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /locus_tag="K0W68_000945"
     CDS             complement(22390..23892)
                     /locus_tag="K0W68_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBI3072592.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="K0W68_000946"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="K0W68_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HBI3072593.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="K0W68_000947"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="K0W68_000947"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBI3072594.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="K0W68_000948"
     CDS             complement(26207..26548)
                     /locus_tag="K0W68_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBI3072595.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="K0W68_000949"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="K0W68_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBI3072596.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="K0W68_000950"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="K0W68_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBI3072597.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="K0W68_000951"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="K0W68_000951"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBI3072598.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="K0W68_000952"
     CDS             33227..34570
                     /locus_tag="K0W68_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBI3072599.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34650..34856)
                     /locus_tag="K0W68_000953"
     CDS             complement(34650..34856)
                     /locus_tag="K0W68_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBI3072600.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35181..35735
                     /locus_tag="K0W68_000954"
     CDS             35181..35735
                     /locus_tag="K0W68_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBI3072601.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35825..36736)
                     /locus_tag="K0W68_000955"
     CDS             complement(35825..36736)
                     /locus_tag="K0W68_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072602.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICKKYKSTIEAFKKEQLALPV"
     gene            complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="K0W68_000956"
     CDS             complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="K0W68_000956"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBI3072603.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37878..39821
                     /gene="cpdB"
                     /locus_tag="K0W68_000957"
     CDS             37878..39821
                     /gene="cpdB"
                     /locus_tag="K0W68_000957"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBI3072604.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39939..40319)
                     /locus_tag="K0W68_000958"
     CDS             complement(39939..40319)
                     /locus_tag="K0W68_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBI3072605.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40408..41268
                     /gene="qorB"
                     /locus_tag="K0W68_000959"
     CDS             40408..41268
                     /gene="qorB"
                     /locus_tag="K0W68_000959"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBI3072606.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41376..42341
                     /locus_tag="K0W68_000960"
     CDS             41376..42341
                     /locus_tag="K0W68_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI3072607.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42449..43111
                     /gene="ytfE"
                     /locus_tag="K0W68_000961"
     CDS             42449..43111
                     /gene="ytfE"
                     /locus_tag="K0W68_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBI3072608.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="K0W68_000962"
                     /gene_synonym="dagA"
     CDS             complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="K0W68_000962"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBI3072609.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44877..45497)
                     /gene="fklB"
                     /locus_tag="K0W68_000963"
     CDS             complement(44877..45497)
                     /gene="fklB"
                     /locus_tag="K0W68_000963"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBI3072610.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45716..46354
                     /gene="ytfB"
                     /locus_tag="K0W68_000964"
     CDS             45716..46354
                     /gene="ytfB"
                     /locus_tag="K0W68_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HBI3072611.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46338..>46686)
                     /locus_tag="K0W68_000965"
     CDS             complement(46338..>46686)
                     /locus_tag="K0W68_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBI3072612.1"
                     /translation="NPWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGK
                     KHNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETIL
                     SWLLVDPSSTAHE"
BASE COUNT        11455 a        12415 c        11632 g        11184 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcaa catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gacccctgaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcactttggt taagccatct ctgtttgcag acgcataacc
    34681 tgacggttaa cttcggacat cactgacaaa tgcagcttat ccttcacttt tgggataaga
    34741 atcttaccct tatcaaattc aaaagcgcca acgtccttga tgtataaccg tccacggaac
    34801 aggatcttta cgtacttcgc cacctgaagt gggttgtacc gttggaaaat tttcattgtt
    34861 atctcctgct gagtattacg cctgtgcggt gccacaatcg gcccaactat tatgaggcgc
    34921 aaattttaat gcttagtgac tatagactat ccgggcaatg tttccaccgc gtataacttt
    34981 ttttacctaa aggttacaat tattcagaat tatcttttta ccgaagcgcg gtcttcagta
    35041 taagcattca tttttcatat gatttgtgcg cttgaccgca aactggcatc acacttgcgg
    35101 gaaattcgat aaatagcaca tatgattaaa actcagaccc aagtggtcgg atcacctgca
    35161 tatcataaga aggaaacacc atgaccctac gcaagattct ggcactcacc tgcctgctgt
    35221 tgccgatgat ggcttccgca catcagttcg aaaccggtca gcgagtgccg ccgattggca
    35281 ttaccgatcg gggcgagttg gtgcttgata aagatcagtt tagctacaaa acctggaaca
    35341 gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa
    35401 aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc gaagttaccg cacgatcgtt
    35461 accagaccac cactattgtt aacaccgacg acgcgattcc gggttcaggg atgtttgtgc
    35521 gcagcagtct ggagagtaat aaaaagcttt atccctggtc gcagtttatt gtcgatagca
    35581 atggcgtcgc gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta gtggtgctgg
    35641 ataaggatgg tcgcgtgcaa tgggccaaag acggggcgct cactcaggaa gaggtgcagc
    35701 aagtgatgga cctgctgcaa aagttgctca aataatggca acaacataga ttctgcaaaa
    35761 ataattttcc cctatcgtta tctatgggat aggggaatta agtctctgag ttcttacgcc
    35821 gaactcatac tggtaacgct agttgctctt ttttaaacgc ctcaatggtc gacttatatt
    35881 tcttgcaaat cttttctttg gtaatattac tatctgcggc cagattcgca cttactatcg
    35941 catccacttg ttcggctaat tccacttgtt ggcaacctgc attaaccaga aaattataaa
    36001 taagggagta atcttcatcc tccaaaacaa cagaggaacc attacgaaaa cgttcatccc
    36061 ctccatagat tttaagcgca aagcccaaag attctatgac tatggcaaaa ttatcatcaa
    36121 ttattttttc gtgaatatac catccatcgc attctttaat tccacctcct ggtatttgga
    36181 gttcaaattt accccgtttt aatttgctta ctatatcctc caataaggca taacctaaag
    36241 acacgtcacc aatcgcctgg tgaatataac ctttaatcct tttcggtatg tcagactgtt
    36301 ttttctgcag aatatcacag gcatacaatc ttctgttttg ttctaacgga aactcgcggt
    36361 ttttgaaatc catgaatgag ttatactcat ttattgttat gggaatttct tcgtattcaa
    36421 atccactacc tcgcaatgtc attgtatatt cggcaccatt atctttaagg ctgaaactga
    36481 taaggaactt atccatggcg ttaacagttc cattttttga tgaaagtacc tgataactat
    36541 ttttatcaat ctgagttgcg caacgaaaga actccctcaa agttaacagg ctatcataat
    36601 tcgaaatcca atcttttttc ttaaatgcat gctggatatt gttaaaaaaa ttgtttgaat
    36661 gtgtttttgt atacgatgac tttacaaata actccaccgc accaataaaa ttattgtgtc
    36721 tgatcctggg taacatatga caaccttatt ttatttggtt attttatgtc gccattaaat
    36781 atctcattag agaagtaaac tatcaaaaaa cgctcattat taaaatgtaa cttattagca
    36841 attgaatttt tgcactttaa atacaatctc tttttattta taacttttta ggttgggaga
    36901 aatcaggatg gcagaatcag gaaatacact gtttctgcca tctgaattta gtaaatagac
    36961 actctgaacc ccggattcag gaacgactcg cgcggagtgt aatccagcgg tttaccctgc
    37021 cagtcgtgaa cgtgcgctcc ggcagctgca gccacagcat gtccagcggc ggtgtcccaa
    37081 atattcgttg gtccgaagcg cgggtacagc tgcgcctgtc cttccgccac caggcagaat
    37141 ttcagcgaag agccgatgga cgtggtctga tgttcgccaa gctgttgcag atactctttc
    37201 agctccgcat ctgcatggga acggctgata accaccagcg gcgggcgcgc atcgcggacc
    37261 tgaatctgct tgcgcacacc gcactcttct ttccaggcct tgccttctgc cgcgctgtac
    37321 attacgttca ttaccggcgc atacaccaca cctaaaatcg gtttgccatg gtcaatgagc
    37381 gcaatgttaa cggtgaattc gccattacgt ttaataaact ctttagtacc atccagcggg
    37441 tctaccagcc agtaacgctg ccagtgctga cggacttccc aaccgggagg atcttcttca
    37501 gaaaggaccg gaatatccgg tgtcagcgta cgtaaaccgt ccatgataac ggtgtgtgcg
    37561 gcaatatccg ctgccgttac cggagaattg tccgctttgc tgacgacgtc catcggtttc
    37621 gtcccgtcgt agacctgcat aatggcatcg cctgcattcc gtgcaagctg gcatacttga
    37681 tctaacattt ctccacctcg tctctgtgag cggtgttaac ttattgtttt acttataccc
    37741 tatcgttaat gaatgcgcca actgtgatag tgtcatcatt ttcaaagcgt aaaattgtgg
    37801 cattcttcac tgttctataa gtaagacgtt tattctccct tttctttcgt attcccgatg
    37861 ataaaaggat gtccctgatg attaagttta gcgcaacgct cctggccacg ctgattgccg
    37921 ccagtgtgaa tgcagcgacg gtcgatctgc gtatcatgga aaccactgat ctgcatagca
    37981 acatgatgga tttcgattat tacaaagaca ccgccacgga aaaattcgga ctggtacgta
    38041 cggcaagcct gattaacgat gcccgcaatg aagtgaaaaa cagcgtactg gtcgataacg
    38101 gcgatttgat tcaggggagt ccgctggcag attacatgtc ggcgaaagga ttaaaaacag
    38161 gtgatgttca tccggtttat aaggcgctga atacgctgga ttatacggtc ggtacactcg
    38221 gcaatcatga atttaactac ggtctggatt acctgaaaaa tgcgctggcg ggagcgaaat
    38281 tcccttatgt aaatgccaac gtcattgacg ccagaaccaa acagccaatg tttacaccgt
    38341 atttaattaa agataccgaa gtggtcgata aagacggaaa aaaacagacg ctgaagattg
    38401 gctatattgg cgtcgtgccg ccgcaaatca tgggctggga taaagctaat ttatccggga
    38461 aagtgacggt gaatgatatt accgaaaccg tgcgcaaata cgtgcctgaa atgcgcgaga
    38521 aaggtgccga tgtcgttgtc gttctggcac attccgggct gtctgccgat ccgtataaag
    38581 tgatggcgga aaactcagtt tattacctca gtgaaattcc gggcgttaac gccattatgt
    38641 ttggtcatgc tcacgccgtt ttcccgggta aagattttgc tgatatcgaa ggggctgata
    38701 tcaccaaagg cacgctgaat ggtgttccgg cggtaatgcc gggcatgtgg ggcgatcatc
    38761 ttggggtggt cgacttacaa ctcagtaatg acagcggtaa atggcaggtg acgcaggcga
    38821 aagcggaagc tcggccgatt tacgacatcg ccaataaaaa atccctcgcg gcggaagaca
    38881 gcaagctggt agaaacactc aaagccgatc acgatgccac acgccagttc gtcagcaagc
    38941 caatcggtaa atctgccgac aatatgtata gctatctggc gctggtgcag gacgatccga
    39001 ccgtgcaagt cgtgaacaac gcgcaaaaag cgtatgtcga acattacatt cagggcgatc
    39061 cggatctggc aaaactgccg gtgctttcag ctgccgcacc gtttaaagtt ggtggtcgca
    39121 aaaatgaccc ggcaagctat gtggaggtgg aaaaaggcca gttgaccttc cgtaatgccg
    39181 ccgatcttta tctctacccc aatacgctga ttgtagtgaa agccagcggt aaagaggtga
    39241 aagagtggct ggagtgctcc gccggacagt ttaaccagat tgatcctaac agcacgaaac
    39301 cacagtcact catcaactgg gatggtttcc gcacttataa ctttgatgtt attgatggtg
    39361 tgaattatca gattgatgtt acccagcccg cccgttatga cggcgagtgc cagatgatta
    39421 ataccaatgc ggaaaggatt aagaacctga cctttaatgg caagccgatt gatccgaacg
    39481 ccatgttcct cgttgccacc aataactatc gcgcttacgg cggcaaattt gccggtacgg
    39541 gcgacagcca tatcgctttt gcttcaccgg atgagaaccg ctcggtgctg gcagcgtgga
    39601 ttgctgatga gtcgaaacgt gcgggggaaa ttcacccggc ggcagataac aactggcgtt
    39661 tagcaccgat agctgccgat aagaaactgg atatccgttt cgaaacttcc ccgtcagata
    39721 aagccgcagc gtttattaaa gagaaagggc agtatccgat gaataaagtc gcgaccgatg
    39781 atatcgggtt tgcgatttat caggtggatt tgagtaagta aaacacttct ttttcggcct
    39841 ataaatcatc aatcgcatcc ggcatttatt ggcggatgcg atgctggcgc atcttatccg
    39901 ccctacaagt catacaccgt aggccgcgca tccggcagtt atgccgcacg ttcatcccgc
    39961 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    40021 ctcacctgct cgcccagcgg cgtgaggctg tattcaacat gcggtggcac caccggatac
    40081 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    40141 tcgctcaccc cgccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    40201 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    40261 cactgttccg caaagagatt accctctttc agttgttgcg acaggctaac ctgactcatt
    40321 tcatacttac ctttttgtac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    40381 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    40441 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    40501 gttcgtaatc cggcaaaagc ccaggctcta gcagcacaag gcattaccgt gcgtcaggct
    40561 gactacggcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    40621 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    40681 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    40741 ctcggcctcg ccgatgagca cattgagacg gagaaaatgc tggctgattc tggcatcgtt
    40801 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    40861 ctggaacacg gcgtatttat cggtgcggcg ggcgatggca aaatcgcctc agcaacgcgg
    40921 gcagattatg cggcagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    40981 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    41041 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    41101 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    41161 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ccgcccaacg
    41221 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    41281 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    41341 tgaactttct tctgacagac caatggatgc cagtaatgat tagcggcgtg ctgtacgccc
    41401 tgttagcagg gttgatgtgg gggcttattt ttgtcgggcc gttgatcgta ccggaatacc
    41461 cggcgatgtt gcagtcgatg gggcgttatc tggcgttagg gttaattgcg ctgcccattg
    41521 cctggctggg acgcgtgcgt ctgcgtcagt tggcgcgtcg cgactggctt accgccttga
    41581 tgctcaccat gatgggcaac cttatctatt acttctgcct tgccagtgcc attcaacgta
    41641 ctggcgcgcc tgtttccacg atgattatcg gcaccctgcc ggtggtgatt cccgtttttg
    41701 ccaatctgct ttatagccag cgcgacggca aactcgcatg gggaaaactc gccccggcac
    41761 tggtttgtat tggcatcggc ctggcgtgtg tgaatattgc tgagttaaac cacggactcc
    41821 ccgattttga ctgggcacgt tatacctctg gcatcgtgct ggcgttagtt tccgtggtct
    41881 gctgggcgtg gtatgccctg cgcaacgccc gctggctgcg ggaaaatccc gacaaacatc
    41941 cgatgatgtg ggcaacggcg caggcgctgg tcacactgcc ggtttctctc atcggctatc
    42001 tcgtcgcctg ttactggctg aatatacaaa cgccggactt ctccttacct tttggccccc
    42061 gtccgctggt gtttattagt ctgatggttg cgatagccgt gctttgctca tgggttggcg
    42121 cactctgctg gaacgtcgcc agccagcgat taccgacagt gattctcggg ccgctgattg
    42181 ttttcgaaac gctggcaggt ttgctgtaca cctttttact ccgccagcaa atgccgccgc
    42241 taatgacgct gagcggtatc gcgctgttag tgattggcgt ggtcattgcg gtcagagcaa
    42301 aaccagaaaa acctttaact gaatctgtct cagaaagttg acacgctggc agtgagttaa
    42361 ataagcctct gctacgtaag ggttatagct tttgccttaa agatgcattt aaaatacatc
    42421 ttatattatt aagaatgagg tatcagctat ggcttatcgc gaccaacctt taggtgaact
    42481 ggcgctctct attcctcgcg cttcagctct gtttcgtaaa tatgatatgg attactgctg
    42541 tggcggtaag caaacgctgg cgcgcgcggc ggcacgtaaa gaactggatg ttgaggtcat
    42601 tgaagctgaa ctggcaaagc ttgctgaaca accgattgag aaagactggc gtagcgcccc
    42661 gctggcagaa atcattgacc atatcatcgt gcgctaccac gatcgtcacc gcgagcaact
    42721 gccggagctg atcctgcaag cgactaaagt cgagcgcgtt cacgccgaca aaccgagcgt
    42781 gccaaaaggg ctgacaaaat acctgactat gctgcatgaa gagctatcca gccacatgat
    42841 gaaagaagag cagatcctct tcccgatgat caaacaaggc atgggcagcc aggcaatggg
    42901 accaatcagc gtgatggaaa gcgagcacga tgaagcgggc gaactgctgg aagtgattaa
    42961 acacaccacc aataacgtca caccgccgcc ggaagcctgc accacctgga aagcgatgta
    43021 taacggtatt aatgaactga ttgatgacct gatggatcac atcagcctgg aaaacaatgt
    43081 actgttccca cgcgcgctgg cgggtgagta atctaaaagc tggatggcat tgcgccatcc
    43141 agcatgataa tgcggttatt tccgcagttc agcagcccgc ttcttaccaa taaacaacca
    43201 gcccagcccc agtgcgataa accacagcgg ggtgaccaac agcgcctggc gagtgtcatc
    43261 ttccagtgtc agcaacacga ccacgaacac aaagaacgcc atacataccc agcacatcag
    43321 cttgccgagc ggcatcttgt agatcgactt ctcatgcaga tgaggacgct gtttgcggta
    43381 aaccaggtac gagcaaagga taattgtcca gacgaacata aacagaatcg cggaaacggt
    43441 tgtaatcatc gtgaacgcgc caatcacgct aggattcaca tacaacatca ccacgccgcc
    43501 gagcagacag atacacgaga aggtcaaccc tttcgcgggt actgcgcgct tagaaagttt
    43561 agcgaacgct ttcggtgcca caccttcctg cgccagacca aacagcatac ggctggtaga
    43621 gaagacgccg ctgttagcgg aagacgccgc agaggtcagc accacaaagt tgatcacgct
    43681 ggcagccgca ggcaaaccta ccaacacaaa cagttcaaca aacgggctct tctccgggac
    43741 taccgaactc cacggcgtca cggacataat cacaatcagc gcgaagacgt agaacataat
    43801 gatacggatc ggaatggagt taatcgcgcg tggcagtgat ttctccggat ctttggtttc
    43861 cgcagcggtt gtacctacca gctcaatccc tacgaaagcg aaaaccgcta tctggaatcc
    43921 ggcaaagaag ccacttaaac ctttcgggaa ccagccgcca tcattccaca aatgcgcgaa
    43981 tgacgcttcc acaccagtcg gtgactgaaa gtgcatcgcc accataacca ggccgacgac
    44041 aatcagcgac acaatggcga cgattttgat catcgcaaac cagaactcca tctcaccgaa
    44101 cattttcacg gtggcgagat tgagcgtcag cagcagcact atcaccgcca gcgaggcgac
    44161 ccagtcggag agatcgggga accagaactg agcataagca gtgatcgcca ccacgtctgc
    44221 cataccggtt acaacccagc agaaccagta agtccagccg gtgaaatatc ctgcccacgg
    44281 cccgagtaaa tcggaagcga agtcactaaa agatttgtat tccagattcg aaagcagcaa
    44341 ttcccccatt gcccgcatca cgaaaaagag cataaaaccg atgatcatat aaacgaaaat
    44401 gatcgacggc ccggcaaggc taatcgtttt gccggacccc ataaacaacc ccgtaccaat
    44461 ggcaccgcca atggcaataa gctgaatatg tcggtttgtg agattgcgcc gtagcgactg
    44521 ttcagccgga gcctgatcat cggcaacgac ttttacctga tctaccatgt ttttttcttc
    44581 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    44641 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    44701 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    44761 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    44821 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    44881 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcgcct
    44941 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    45001 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    45061 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    45121 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    45181 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    45241 ttcacacctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    45301 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    45361 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    45421 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    45481 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    45541 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    45601 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    45661 gttagcttat tcataggcta tgattgagga acaagacgcg gagcaggagg aaaccatgcc
    45721 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    45781 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    45841 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgccggtgg tgacgcgtga
    45901 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc tgcgggcgca
    45961 attagtcaca ccacaaaatg atcctgatca ggtggctccg gtcgctccag aacctataca
    46021 ggaaggccag ccagaagaac agccgcaaac tacccaaacg caaccattcc agccagatag
    46081 cggaattgat aatcaatggc gctcttaccg tgtcgaaccc ggtaaaacga tggcgcagct
    46141 gttccgcgac cacggactgc ccgctaccga tgtctatgca atggcgcagg tagaaggcgc
    46201 gggtaagccg ctgagtaatt tgcaaaatgg gcagatggtg aaaatccgcc agaacgccag
    46261 cggcgtggtg acgggcttaa ccattgatac ggggaataat caacaggtcc tgtttacccg
    46321 ccagccggat ggcagtttta ttcgtgcgcg gtagatgaag ggtcaaccaa aagccagcta
    46381 aggattgtct ctttgatgac cttacgtctg gcttccagtg cctggggtga aaccatactg
    46441 atgtgataaa ccaggccaag cgtatgctgg ttgatcaaat aatatccccc aagcgcagca
    46501 atattaatat tcacctgcaa tgggtcgata tctggcttaa aaatattgtg tttcttccct
    46561 tcatccaata aggattccat taactgcaag tgcgcatggt taatttctaa gagacgcagt
    46621 gattttgcat aatgcacacc tttactttga ttttcactat gcacaatttt taaaaaccaa
    46681 gggtta
//
LOCUS       DAETQN010000016        45922 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.016, whole genome shotgun sequence.
ACCESSION   DAETQN010000016 DAETQN010000000
VERSION     DAETQN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.016"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..715)
                     /locus_tag="K0W68_000966"
     CDS             complement(<1..715)
                     /locus_tag="K0W68_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBI3072613.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSS"
     gene            complement(729..1388)
                     /gene="pgmB"
                     /locus_tag="K0W68_000967"
     CDS             complement(729..1388)
                     /gene="pgmB"
                     /locus_tag="K0W68_000967"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBI3072614.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1385..3652)
                     /gene="ycjT"
                     /locus_tag="K0W68_000968"
     CDS             complement(1385..3652)
                     /gene="ycjT"
                     /locus_tag="K0W68_000968"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBI3072615.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3649..4704)
                     /locus_tag="K0W68_000969"
     CDS             complement(3649..4704)
                     /locus_tag="K0W68_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBI3072616.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4714..5502)
                     /locus_tag="K0W68_000970"
     CDS             complement(4714..5502)
                     /locus_tag="K0W68_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBI3072617.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5520..6572)
                     /locus_tag="K0W68_000971"
     CDS             complement(5520..6572)
                     /locus_tag="K0W68_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBI3072618.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6603..7206)
                     /locus_tag="K0W68_000972"
                     /pseudo
     CDS             complement(6603..7206)
                     /locus_tag="K0W68_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(7193..8074)
                     /locus_tag="K0W68_000973"
     CDS             complement(7193..8074)
                     /locus_tag="K0W68_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBI3072619.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8095..9405)
                     /locus_tag="K0W68_000974"
     CDS             complement(8095..9405)
                     /locus_tag="K0W68_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3072620.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIVLPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9440..10950)
                     /locus_tag="K0W68_000975"
                     /pseudo
     CDS             complement(9440..10950)
                     /locus_tag="K0W68_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(11162..11476)
                     /gene="pspE"
                     /locus_tag="K0W68_000976"
     CDS             complement(11162..11476)
                     /gene="pspE"
                     /locus_tag="K0W68_000976"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBI3072621.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11551..11772)
                     /gene="pspD"
                     /locus_tag="K0W68_000977"
     CDS             complement(11551..11772)
                     /gene="pspD"
                     /locus_tag="K0W68_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBI3072622.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11781..12140)
                     /gene="pspC"
                     /locus_tag="K0W68_000978"
     CDS             complement(11781..12140)
                     /gene="pspC"
                     /locus_tag="K0W68_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBI3072623.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMLDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12140..12364)
                     /gene="pspB"
                     /locus_tag="K0W68_000979"
     CDS             complement(12140..12364)
                     /gene="pspB"
                     /locus_tag="K0W68_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBI3072624.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12418..13086)
                     /gene="pspA"
                     /locus_tag="K0W68_000980"
     CDS             complement(12418..13086)
                     /gene="pspA"
                     /locus_tag="K0W68_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBI3072625.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13253..14230
                     /gene="pspF"
                     /locus_tag="K0W68_000981"
     CDS             13253..14230
                     /gene="pspF"
                     /locus_tag="K0W68_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBI3072626.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14348..15613)
                     /gene="puuE"
                     /locus_tag="K0W68_000982"
                     /pseudo
     CDS             complement(14348..15613)
                     /gene="puuE"
                     /locus_tag="K0W68_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15651..16931)
                     /gene="puuB"
                     /locus_tag="K0W68_000983"
     CDS             complement(15651..16931)
                     /gene="puuB"
                     /locus_tag="K0W68_000983"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBI3072627.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16933..18423)
                     /gene="puuC"
                     /locus_tag="K0W68_000984"
                     /pseudo
     CDS             complement(16933..18423)
                     /gene="puuC"
                     /locus_tag="K0W68_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(18573..19130)
                     /gene="puuR"
                     /locus_tag="K0W68_000985"
     CDS             complement(18573..19130)
                     /gene="puuR"
                     /locus_tag="K0W68_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBI3072628.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19157..19921)
                     /gene="puuD"
                     /locus_tag="K0W68_000986"
     CDS             complement(19157..19921)
                     /gene="puuD"
                     /locus_tag="K0W68_000986"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBI3072629.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20133..21494
                     /gene="puuA"
                     /locus_tag="K0W68_000987"
     CDS             20133..21494
                     /gene="puuA"
                     /locus_tag="K0W68_000987"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HBI3072630.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21643..21807
                     /locus_tag="K0W68_000988"
     CDS             21643..21807
                     /locus_tag="K0W68_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072631.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21797..23182
                     /gene="puuP"
                     /locus_tag="K0W68_000989"
     CDS             21797..23182
                     /gene="puuP"
                     /locus_tag="K0W68_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HBI3072632.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23316..23561
                     /locus_tag="K0W68_000990"
     CDS             23316..23561
                     /locus_tag="K0W68_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBI3072633.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23874..25517
                     /gene="sapA"
                     /locus_tag="K0W68_000991"
     CDS             23874..25517
                     /gene="sapA"
                     /locus_tag="K0W68_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBI3072634.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25514..26479
                     /gene="sapB"
                     /locus_tag="K0W68_000992"
     CDS             25514..26479
                     /gene="sapB"
                     /locus_tag="K0W68_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBI3072635.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26466..27356
                     /gene="sapC"
                     /locus_tag="K0W68_000993"
     CDS             26466..27356
                     /gene="sapC"
                     /locus_tag="K0W68_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBI3072636.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27356..28348
                     /gene="sapD"
                     /locus_tag="K0W68_000994"
     CDS             27356..28348
                     /gene="sapD"
                     /locus_tag="K0W68_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBI3072637.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28350..29156
                     /gene="sapF"
                     /locus_tag="K0W68_000995"
     CDS             28350..29156
                     /gene="sapF"
                     /locus_tag="K0W68_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBI3072638.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29224..29577
                     /locus_tag="K0W68_000996"
     CDS             29224..29577
                     /locus_tag="K0W68_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBI3072639.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29945..30733
                     /gene="fabI"
                     /locus_tag="K0W68_000997"
     CDS             29945..30733
                     /gene="fabI"
                     /locus_tag="K0W68_000997"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBI3072640.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30878..32004
                     /locus_tag="K0W68_000998"
                     /pseudo
     CDS             30878..32004
                     /locus_tag="K0W68_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32072..34006
                     /gene="rnb"
                     /locus_tag="K0W68_000999"
     CDS             32072..34006
                     /gene="rnb"
                     /locus_tag="K0W68_000999"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBI3072641.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34084..34187)
                     /locus_tag="K0W68_001000"
                     /pseudo
     CDS             complement(34084..34187)
                     /locus_tag="K0W68_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34241..36231
                     /gene="pdeR"
                     /locus_tag="K0W68_001001"
                     /pseudo
     CDS             34241..36231
                     /gene="pdeR"
                     /locus_tag="K0W68_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            36378..36551
                     /locus_tag="K0W68_001002"
     CDS             36378..36551
                     /locus_tag="K0W68_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072642.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36641..37390
                     /locus_tag="K0W68_001003"
     CDS             36641..37390
                     /locus_tag="K0W68_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI3072643.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37659..37877
                     /gene="osmB"
                     /locus_tag="K0W68_001004"
     CDS             37659..37877
                     /gene="osmB"
                     /locus_tag="K0W68_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBI3072644.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38003..38329)
                     /gene="yciH"
                     /locus_tag="K0W68_001005"
     CDS             complement(38003..38329)
                     /gene="yciH"
                     /locus_tag="K0W68_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBI3072645.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38329..39066)
                     /gene="pyrF"
                     /locus_tag="K0W68_001006"
     CDS             complement(38329..39066)
                     /gene="pyrF"
                     /locus_tag="K0W68_001006"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBI3072646.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39259..40428)
                     /gene="lapB"
                     /locus_tag="K0W68_001007"
     CDS             complement(39259..40428)
                     /gene="lapB"
                     /locus_tag="K0W68_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBI3072647.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40435..40743)
                     /gene="lapA"
                     /locus_tag="K0W68_001008"
     CDS             complement(40435..40743)
                     /gene="lapA"
                     /locus_tag="K0W68_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBI3072648.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(40892..41656)
                     /gene="pgpB"
                     /locus_tag="K0W68_001009"
     CDS             complement(40892..41656)
                     /gene="pgpB"
                     /locus_tag="K0W68_001009"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBI3072649.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            41826..42416
                     /gene="ribA"
                     /locus_tag="K0W68_001010"
     CDS             41826..42416
                     /gene="ribA"
                     /locus_tag="K0W68_001010"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBI3072650.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(42480..45155)
                     /gene="acnA"
                     /locus_tag="K0W68_001011"
     CDS             complement(42480..45155)
                     /gene="acnA"
                     /locus_tag="K0W68_001011"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBI3072651.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45319..45414)
                     /locus_tag="K0W68_001012"
     CDS             complement(45319..45414)
                     /locus_tag="K0W68_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072652.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45528..45695)
                     /locus_tag="K0W68_001013"
     CDS             complement(45528..45695)
                     /locus_tag="K0W68_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072653.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45698..45862)
                     /locus_tag="K0W68_001014"
     CDS             complement(45698..45862)
                     /locus_tag="K0W68_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBI3072654.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT        10848 a        12140 c        11559 g        11375 t
ORIGIN      
        1 ccgatgatcc aataaatccg gcaacaaaca tattcgctgg ttggttataa accgttttcg
       61 gcgcaccaac ttgctgaaca atcccatcct tcataatcac aatccgcgtc gccatggtca
      121 tcgcttcggt ctgatcgtgg gtcacgtaga tcatggtggt attcagcttc tggtgcagct
      181 tgctgatctc tgcgcgcatt tgcacgcgca gcttggcatc aaggttagag agcggttcat
      241 ccattaaaaa cacgcccgct tcgcgcacaa tcgcccgccc gagcgccaca cgctggcgtt
      301 gcccgccgga aagcgcgccc ggcttgcgtt tcaggtactc acgcaggccg agaatttgcg
      361 ccgcccagtt cacccgctca tcaatcacct ctttggcgat tttttgcatc ttcaggccaa
      421 acgccatgtt gtcgtaaacc gtcatatgcg gatacaacgc gtagttctgg aacaccatcg
      481 ctatattgcg tgctttggct ggaacgtcat tcattcgttt gccgtcgatc aacagatcgc
      541 cgccgctgat ctcctcaagc ccggcaatca tgcgcagggt ggtcgactta ccgcagcctg
      601 acggaccgac aaacacgatg aactctttat cggcaatttc caggttgaag tccttcacca
      661 catgcacctg gttatcgtag attttttgaa tatgttgtaa cgaaagctga gccatgttga
      721 ttcctttgct atacgttttg ccagaaggcc gataaccgcg accaggtcag ggagtccgtt
      781 gaaggcaata gtaattgcgc cccggttatg cccgcgccga tccccaccga gcgcataccg
      841 ctggcgttaa tggcgtcaat gcccgcctgc gcatcttcaa tgccgataca tgcctgcggc
      901 ggcacgccca gcccggcaca ggcagcgaga aagatttccg gatccggttt cgagttttta
      961 agttgggaag catccgcgca gaaggtgaaa aactcacgca gctccagtgc atctaaaatc
     1021 gtcggcgcat tgagggagac agaagccagc cctaccgaga tctgttgtgc gcggagatct
     1081 gccagcaaag agcgaatgcc gggtagaaca gcgttgaccg tcaactcgcg caatgagtgg
     1141 acatagagca gatttttgcg atacgccagt tgcgccctct cctgcgagtt aaagtcgccc
     1201 tctttgcccc cgtgttgcag aatgcgccgc agagactcat cgcggctgat cccttttagg
     1261 gattcgttaa actgcgcatc aatgctgatg ccaatttcag cggcaatctg ctgccacgcc
     1321 tggaaatgca gatgcgcggt atcggtgatt acaccatcca gatcgaaaat tacctcttgc
     1381 agtttcattc atcctcctga tgtgtggcag cggtcccatt gaagggcaaa ataaaatcac
     1441 ccaaacagaa aacagattct tcagccacgg atataagctg accgttcaaa cgcagtgaaa
     1501 cgggggcaga agttcgaatc gcaatacgct gcgcgtcaag agtgacctgt aattcgcagc
     1561 cctgccagaa cagagggaaa gacaactgtt gccactgctc aggtaacgcc ggattgagat
     1621 gcaattcacc gtcacgcacg ctcaccccgg caaaaccctg aatcgccccc agccagatag
     1681 cgccagttgc ggcagcatgg ataccatcat cacaactatg cggatcagca ccaagatcga
     1741 tttcagtccc ctcgcgccag aactgataac tttgggtcag caggccacag cgtgcggcaa
     1801 caatgccgtg gattgcttta cttaatgacg agtcgtgaat agtgcgcggt tcataaaatt
     1861 gcagattggc aagacacgat gccgctgaga actgctccgg cagcatgtaa ttgagcatca
     1921 ccacatcagc ttgtttgagg atttgcatct cgttcacttc tgcgcgtgaa taatccagca
     1981 gaatggtttg cttccccgcc gccgctttgt atttcgcaag attaatcgcc ggcttagcca
     2041 taaacgaatc atcctgcggc aaaacgccgt cgggctgaat ttctggcagc cgcagttctt
     2101 taaggaacat ttcggcacga tgaataaacg cgtcgtcgct acagccaaac tggcgggcaa
     2161 tactcagcgc ctgctggacg ttgtaatacg ccatatagct ggtgaaggcg ttattattga
     2221 catgttcggt atattcgtca ggcccaataa catcatgaat ttccagacgg tcgttaaccc
     2281 tcaccgcgcg gctaatccag aactttgcag tttccagaag tagcgccatg ccttcatgcg
     2341 caatgaaact ttcatccccc gtggtctgcc agtattgaat aaccgcccag gcgatatcgg
     2401 ccaccagatg atgttccgcc tgcgccgagg ccactttttg ccgcagcccg gtgcgaatgt
     2461 taatggcggc aaattccggc gtctcttctt cgccgctgcg cgcgctttcc cacggaaata
     2521 gcgcgccctg ccagccgttg cgtcgcgctt tctcctgcgc gcctggcaag ttgtgccagc
     2581 gataacgcag taaacttcgg gcaaccgtcg gatcgctaaa cagatgaaac ggtaacaaaa
     2641 atacttctgt atcccagaaa acgtggcctt tgtagccttc gccggttaag ccttttgccg
     2701 caatgctgct gcgctcgtcg tgggcaggcg tcatgatgcg cagatgataa agcgcataat
     2761 ctaacgcttg ctgatcgtgc acttcgccgc catttaccgt gatacgacgt ttctgccacc
     2821 attgacgcca gttttctgtt gatactgcaa gaagttggtc gtaactctgc tgcgcgcaca
     2881 tttcaagctg gcgaagcgac gcgctgcccc actcgtctaa aacggcttgc ctgtcatccc
     2941 gccagtcgat ccacaccagt ttttgcaacg tcactgtctc gcctgcatga agctgcgcac
     3001 tggtatgttg cagtaaacgg cgctctttgg cggtatagca ttgctgcaca tcaccgctca
     3061 ccttacagca acagctgatg gccacatcac tgcgtccatc ctgggtggtg tagatcccct
     3121 gcatcaaatg ctgaccaaac acccgcacct gggtttcgtc gagatgctgg cgaccgtggt
     3181 tggtctgcgt ggcgtcaatg cctgttgaaa tcagcactga cgcgtcggcg tccagtggcg
     3241 taatagtgat ttccagcaca atgagcggca gttggtctgc cgaaacaaag cgacggctgg
     3301 cgatggtgta acctgtgccg ttgctggtac gccagacaac gctacggcgt aattctccac
     3361 tggcaaagtc aagttcacgc tgccaggctt cgcgggataa cgagaaaacc tcaccattta
     3421 tggcaatctc catccccacg acatcaggca ggttcaccag ttcgttgatt tcaccttttc
     3481 ccgcccgatg atacagcccc gccagataca tccctcgcgt ctggcgcgtg taatcttctt
     3541 catggctggc gcgaagccca agatagccgt tcccctgcgc catcagcgat gcatacttgt
     3601 tcagggtatg ctggctgaaa tggggttctg ataacgttac tggcctggtc ataattccac
     3661 acgcgtccct gtttctgcgg attgatacaa cgccgccacc agttgctgga tgatgtaccc
     3721 ctgcttggcg tcggctatca tcacgggctt gccctgtacg tgattgataa aggcttccat
     3781 gctgcgcaaa tggcggttgt cgtctgccat tttccgttgc atcagcgtca ttaattcacc
     3841 gttgttatcg gtgtagatat gtgctggaaa cagcgtcgca ccagctttat caccacaaaa
     3901 gctgacgttc ataatcgact gttcgcggat gttgagtgca aatgacgttt ccagccacag
     3961 aatgccgccg ttatgaaatt caatggtgcc aaacagcgaa tcttcgacgc tgtaagttgc
     4021 cggatcccac tcaccaaatt gaccacagct cttttgcgtg ccgatctttt gaaagctatg
     4081 cgcattcacg cttttcaccg ccggaaagcc cagcacatac atcgcagcat ccagcatatg
     4141 aatgccgatg tcgatcagcg ggccaccacc ctgcagttct ttattggtaa agacacccca
     4201 gccgggaacg ccgcagcgac gcagggcgcg ggcggtggta acgtaaatct ctcccaaaac
     4261 gccgttggtc acctgttcac gcagctgttg cgtatcgagc gcaaaacgat ggtgaaagtc
     4321 gtaggccagc accttgccca gtttgcgcgc ggtatcgcac atttcccgcg cctgttctgg
     4381 cgtcatggcg agcggttttt cgcacatcac atggcagccc gcttccagtg ccatcagggt
     4441 atgttcgtaa tgaaaacggt taggtgagca gacgctaacc acatcaggtt tcaccgccag
     4501 cagcatggcc tgcggatcgt cccacacgga tgcattcccg tatttttctg ccagcgcctg
     4561 cgcctgggaa aggcggctgt cacagacagc caccagttcc agatcgttgc gggtgcagta
     4621 gtacgaagca tgaaccttat ccgccacctg gcctgcgcct attatcgcga cccgcagcgg
     4681 agagcttgtc attgcacttt tcacaaagac ctcttagcag gtacgcaacc aggccaacga
     4741 atcacggtac gcctgggcag gatcttccgc ccggatacgc ccttcgtaca ctacatagcc
     4801 ctgatagtta tccgcgcgca gctgttcaaa cagcgcgtgg aaatccaggg tgccgctgcc
     4861 cggctggtag cgatggttat ccgcaatatg cacatgaccg agcaggtcgc ggttgtcatg
     4921 cagcgcctgc gccaggttat cttcttcgat attcatgtga tagaaatcgc cgataatctg
     4981 tacatgttta agatcgtttt cgacgatgta acggcgggca tcggcgaggg tgttgatcat
     5041 atgatcctga tagcggttta acggttcgag atacaccact gttccggtac gcgcggcgac
     5101 ctgttccagt acgcgcaggg aatcactcac cattttgcgg tcgccgtcca ggctacgcgg
     5161 cgaggtcatc ggcggtaagc ggaaggtaaa catgccccac gctgccggaa cgacgatacc
     5221 tttatccccc acttctgcca gcgcttcgag aatgcgttcg atctgcttta agccattaag
     5281 acgacgctct tcgataaagt cgccaatcca accgtcatag ccaccgcagg cggtggtcac
     5341 cggtaaaccg gtttctttga ttgctgcttt gacttcttcg agattgttga ccagcagttt
     5401 gccgtcaatc tcaaaaccat cgaagcccat ctctttgata taacgaaatt tctccagaat
     5461 gttttccgga aagaatgcct gattctgtgt gcctattttc attttttcat tcctttgaat
     5521 taaaacgtaa cgcccatttt gatgctctgt tccggatgct gatcgacata ctgcatgtag
     5581 ctttccgggc tattggcaaa ggtcactacc gggtcgatta aatcttcgca attgagataa
     5641 ccgttcatca gcagttccca gcaggtttct tcaatacgct tacggctcca gcgcggataa
     5701 tccgggttcg gttcgctgca cgcgcgggag aaaacaattt tggcgttatt gaaatgcgct
     5761 tcgcgtccga ggttaaaacc ttcagcaaat ggtttggcaa acgcaacata ggagatggtg
     5821 ccaccgtagg ccagaccgcg gaacgccgat tgcagcgcgt cggcatagcc gctggtttcg
     5881 ataatcacat cggcaccctg cttgccggtc agcgttttga tctctttgcc tacgtcagta
     5941 ccaatggggt taaggcagaa atccgcgccg tggcgacggg caatatcaca gcgatgagcg
     6001 ataggatcga cgccaatcac gaccgacgcg ccagcacgtt tagccagttg aatggcaatt
     6061 tgaccaatcg cgccaagccc taccaccaca acaaaatcac ccacgcgcac gttggcatcg
     6121 cgcacgccgc tcatggcaaa ctgcgccggg tcgtaacaaa cggcgttttt ccacgagctg
     6181 ccttccggca ttttgcgcag cttgtagtta ttcaccgcgt taatgatgac cgtctcggag
     6241 agcgggccgt agccgcatac gctgtcgccc accgcgtagt cggtaacgtc gctgccgcac
     6301 tcgataatgt cgccaaccac catgtttcca agctggaatt tgccaaactc aatgccgcgc
     6361 ggcgcatctg ccggacgcgg agtgaacatc tgccattcgc cgttaaagtc ttcatcgata
     6421 aacgggctgg cggcgcggaa gtcgaccact tccgttccgt gtttcggtgc gccgaaacgc
     6481 acgcggacct tcacttcatt agctaaaatc gcccgatctt catactcaac cagcgcagca
     6541 acacgcggtg ctgtggctac taactttttc atttaaaact ccttgtaaag aaactggccg
     6601 ctttagccct tcacgccacc ggcggtcaaa ccactcttaa taaaacgttc cgaaagcgca
     6661 tacataatca ccaccggcaa tgcggtcacc agtgaggccg ccatcatccg tccccagatg
     6721 taatctggcg tactgaacag cgcgttcagg cccaccggta aggtgaaatt gccggcgctg
     6781 gagaggaaaa tcgacgcaaa cagatagtcg ttccacgcca ccataaagca gtagacgaac
     6841 acggatatca gcccggacat cgccaacggc acggtgatgc ggaagataat ttgcagacgg
     6901 ttgaggccat ccatcatcgc cgcttcttcg atctcatcgg ggatggtgtc gaagtagctt
     6961 ttcagcataa acacggcggt gggcagagtt tgcgtcacca tggtgatgat cagcgccatc
     7021 tcagtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
     7081 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
     7141 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
     7201 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
     7261 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
     7321 gatagacgcc gagaatatcg accttggtgg tcagcagata aacatcagca aacatgtaga
     7381 acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
     7441 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
     7501 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
     7561 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
     7621 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
     7681 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
     7741 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccactgcca
     7801 gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc atccacagcg
     7861 agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca acaaaggttg
     7921 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccattg
     7981 gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc agcgcaaagg
     8041 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
     8101 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
     8161 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaacccg tcacatcacc
     8221 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
     8281 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
     8341 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
     8401 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
     8461 ggccttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
     8521 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatta cagccggaag
     8581 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
     8641 catgatgtcg ttagaacccg gcatgtcgtt agaacccggc atagtgttgg cagcaaggtc
     8701 gcggtaatag gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc
     8761 gttaaagaca ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac
     8821 gctttctgct gtaggcagca caatgccgta ttttttattc gccgggtcat tcagtttctg
     8881 tgcaacgtcc agtagctgtt gccagttttt cggctcctcc agccccgctt ttgccagcac
     8941 atctttgcga taccagatgc cgccaatcca ggcgctgacg ggaacaccgg tccaggcact
     9001 cccatcttcg gtgcgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt
     9061 gctgatgacc gtggcaaccg tcttgcgatc gataagctgc tctttatcca taactttggc
     9121 gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac
     9181 tttggtgtta taagcgtcct cttccacggg cacttgttta acggtgatac ctgggttttc
     9241 tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct ccaccgaaga
     9301 atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg aaatcagggc
     9361 gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt
     9421 atttaatcca cataacctgt taggggcgca gcgttatttc attacccaat agatcaactt
     9481 cactaatcag atcccgacca tgcagattag taatatttac atgctgaata tttttactga
     9541 cattaaacaa tccagtcaga caatcaccat cagcgttact tctttgaata cgcattacgg
     9601 atgaattaat cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac
     9661 gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt
     9721 tcagttctcg ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc
     9781 cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg
     9841 ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt
     9901 cttcatcgct actctcacgg cggcttaacg catccatatg tgagaggcga ggaagttaaa
     9961 ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac
    10021 gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg
    10081 tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc
    10141 ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
    10201 cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac
    10261 ggcatccagg cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
    10321 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
    10381 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
    10441 cgggcgagtg acggcgctga gatcggtttg cggatcaacc gcaataaaaa aatcttcaaa
    10501 acctggctgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca
    10561 gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc
    10621 cgcttcactg gcgacctgat gataatcaat taccgaaaac ccatcatcag atgaacatgg
    10681 ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg
    10741 attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa
    10801 cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact
    10861 ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc
    10921 gtccaggtaa tccgtaattt tctgtttcat gttgcctttt tgtggtagcg gtcccatgaa
    10981 acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca
    11041 ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg
    11101 gatgtgaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    11161 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    11221 tatatcccat ctcgctaagg atctcttttg cttgccctga ctggcgtccg gcattgcaat
    11281 acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct
    11341 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    11401 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    11461 agcctttttt aaacatgtaa gcgtccttct atggatgaaa attgccatcc attatacgcc
    11521 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgtg ccagtttatt
    11581 agcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    11641 agcaactgat tttatcgccc agcccgctac acccgccggg ccatagcgca gtgcggtaag
    11701 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgcccgg cctgttgcca
    11761 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaaagtatcg
    11821 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    11881 acttcatcca gcaattcgct gctggaaggt agctgctcac caaaggccat gttgtccagc
    11941 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    12001 aagaaaatcg acagcaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    12061 gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg gaatacgcca taatttttta
    12121 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    12181 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    12241 gctgctgctc actttgcgac aattcatcgc gaccagaacg attgctgtaa tgcagccata
    12301 accagatcgg taaaacaaac agcacaaaaa tggttaacgg aatagccaga aatagcgcgc
    12361 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    12421 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc
    12481 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    12541 tgcttccgct tccatctggt caattcgacg ttcgaaagat tcgaaacgag ccattgcttc
    12601 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    12661 acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    12721 aatctctttc ttcatgcgtg ccagcgtatc gttcaccagc gtcacttcat gttccaggga
    12781 cttaatcaga tcggtcagtt tctgtttttc aattaacgct gcacgtgcca gatcctctct
    12841 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    12901 ttcaatacgg cgagtcagct gtttcttttc tgccaacgca cgcgccgaag tagaacgtac
    12961 ttcaaccagt gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    13021 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    13081 acccataatg ttgtcctctt gatttctgcg ttcaggattg tcctgctgaa ctgttaatac
    13141 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    13201 caacatgcca ggatgaatta gcgaattaca ctaacaagtg gcgaatttca tcatggccga
    13261 atacaaagat aatttacttg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    13321 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    13381 gctgattgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    13441 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    13501 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg acggcggtac
    13561 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    13621 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg
    13681 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc
    13741 tgacctgctc gaccgactgg cttttgatgt tgtacaactg ccaccactgc gcgagcgcga
    13801 aagcgacata atgttgatgg cagaacactt tgccatccag atgtgtcggg aaatcaagct
    13861 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    13921 gggaaatatt cgtgaattga aaaacgcggt ggaacgttca gtgtatcgcc acggcaccag
    13981 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    14041 tatcgccgtt tcagaaacca cctcgcttcc gacgctgccg ctggatttac gtgagtttca
    14101 aatgcagcag gaaaaagagt tgctgcaact cagtttgcaa aaggggaaat ataaccagaa
    14161 gcgcgcagct gaattgctgg ggttaaccta ccatcagttc cgcgcgttgt tgaaaaaaca
    14221 ccagatttag cgaagttggg caatgcgatt cgccggatgc ggcgtgaacg ctttatccgg
    14281 catacataat caacgtcgtt tgcaggcctg ataagcgcag cgcatcagac attattgcgt
    14341 tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg aattgcgcat
    14401 ccgggatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca
    14461 ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc gctgacggct
    14521 cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg cgtaccgcag
    14581 caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt
    14641 gattcgcgcg ttcgcagagt gattctttgt tgataatatt gagcaccgcg tgcgcggcag
    14701 ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca
    14761 taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca
    14821 tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta ccggtacgcg
    14881 caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc
    14941 gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt
    15001 cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag cgttcgatgg
    15061 cgtcgaggga gtcctatgtt gaaatgccgt gtaaatctga cggataaggt acgtgataca
    15121 ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg gtcagcgcca
    15181 tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca gggcgtccgg
    15241 tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta gtgaagaacg
    15301 cggttttggc ctgcccgctc accggggcaa gggcgttgat tttctccgcc agggtgacgt
    15361 agctttcata cggcacaatc tgatacgcgg tgtgggtaaa ctgttgcagt tgctgctcca
    15421 ccgccgcgac cagatcaggg tggcgatgtc cggtattcag caccgcaatg cctgcggcga
    15481 aatcgatgta ctcgttgccc tcaacatcct tcagagtagc gttttcagcc gactgggcga
    15541 agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc tgatggaatt
    15601 cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa ttaaacgccc
    15661 agacgatcgc gcaggctgta ccaggctgcg cccatcgcgg taaacggtac tcgcagcgtg
    15721 cgcccgccgg ggaacgggta atacggcaga ttggcgaagg catcgaaacg ttcggcgtcg
    15781 ccgcgcagca gttcggcaat caaacgtccg gcgagatgag tacaggtgac gccgtggccg
    15841 ctgtaaccct gcatgtaata gatgttggta tcaaggcgac caaactgcgg catgcgcgac
    15901 agggtcagca ggaagttgcc cgtccagcgg tagtcgattt tgacgccctt cagttgcggg
    15961 aaggttttca gcagtttcgg cacaacaaga cgctcaacgt catccgggtc gcgcgcgccg
    16021 tagaccacgc cgccgccgta cagcaaacgg ttgtcggcgg taagacggta gtaatcaagc
    16081 agataattac agtcttccac acagtagttt ttcgggatca gcgaacgggc taaatcttcc
    16141 gacagccgtt cagtggtgat cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc
    16201 ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt cgccgtcacc
    16261 tgacctttgg cagtgcgcac caccgctggc gtggtgtgct ggatctgcgt cacggcagaa
    16321 agctcataca cgcgcccgcc gttcaggcgg atggcatcgg cttcaccaat ggcgaggttt
    16381 agcgggtgaa tatgcccacc gctgtgatcc aacagtgcgc cagtgtagcg atcgctggcg
    16441 acctcgcggc gaatggcgtt agcgtccagc agctccagct gtttattgcc gtagcgttcc
    16501 cagttctctt tctgctcttc gagtgtggcg agctgtttat cattcatcgc cacaaacagg
    16561 ccgccggggc ggtagtcgca gtcaatctga tagcgtttga tacgttcgcg gatgatctcg
    16621 ccaccctcga acatcatgct gccgagcata cgggcggtgt ccatgccgta gcttttttcg
    16681 atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg cccgcttgcg
    16741 ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc cgccagatgc
    16801 agcgcggagg agagcccggt atagccgccg ccaaccacgc aaacgtcgca ggtgattgac
    16861 tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg cggcgtagta actgctggta
    16921 tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcag tgaatttttc
    16981 aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc caaacggcac
    17041 ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccggctttca ggcggcggct
    17101 catacggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc cgtactggct
    17161 gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca ccagcaccgg
    17221 accgaaaatc tcttcgcggc ttaaggacgc attcgggtca acatccacaa agatggtcgg
    17281 gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc ctttgctttc
    17341 gccttcacga ataaagctat ggaccgagtc ggcgtgggcg caatcgatta aggtgcccat
    17401 ggtggttacg gtatcaagtg gatggcccgg ctgccagttt tgcgcctgct gttttaacag
    17461 ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg cgatgcacac
    17521 ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt gcagatccgg
    17581 acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga cgcgtttcat
    17641 gttgctatca cccgcgtctt tcagcagttg tttacccgta cgcgtggagc cggtaaaggc
    17701 gatggcatcg atatcgttat gacgcgaaag cgcctgcccg gcttcatgac cataaccgct
    17761 cactacgttc agcacgccat ccggcagccc cgcttcttct tttgccagcc cggcgagacg
    17821 aatcgcactg agtggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag
    17881 cgccgggccg agtttccagc aagtcagcag cagcgggaag ttccacggca cgatggcggc
    17941 aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac
    18001 ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg
    18061 aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc
    18121 cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt
    18181 agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc
    18241 atggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct aggtgaccgg
    18301 atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa
    18361 gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt
    18421 cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag
    18481 agatccccgg gccgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc
    18541 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    18601 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    18661 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    18721 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    18781 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
    18841 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    18901 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    18961 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    19021 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    19081 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    19141 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    19201 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    19261 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    19321 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag
    19381 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    19441 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    19501 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    19561 ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    19621 taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    19681 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    19741 tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca
    19801 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
    19861 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    19921 tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat
    19981 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    20041 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    20101 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    20161 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    20221 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    20281 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt
    20341 tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg
    20401 gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt
    20461 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    20521 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc
    20581 gcgacgctga agggaatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    20641 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    20701 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    20761 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    20821 tggcactaaa acgcctggtg cgtctgatgg caggaaagca taagatgcac gccactttta
    20881 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    20941 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    21001 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    21061 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    21121 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    21181 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    21241 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    21301 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    21361 agaatgatca cctgcgccgc tatttaggca aacggttttg ccatgtgtat cacgcctgta
    21421 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    21481 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    21541 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    21601 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    21661 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    21721 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    21781 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    21841 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    21901 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    21961 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    22021 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    22081 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    22141 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    22201 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    22261 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    22321 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    22381 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    22441 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    22501 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    22561 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    22621 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    22681 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    22741 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    22801 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    22861 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    22921 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    22981 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    23041 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    23101 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    23161 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    23221 cgcgccgcgc cattaactat aaaaagatgt ataacccgga tcacgagtgc tatcatccgc
    23281 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    23341 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    23401 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    23461 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    23521 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    23581 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    23641 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    23701 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    23761 ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca
    23821 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    23881 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    23941 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    24001 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    24061 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    24121 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    24181 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    24241 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    24301 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    24361 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    24421 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    24481 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    24541 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    24601 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    24661 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    24721 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    24781 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    24841 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    24901 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    24961 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    25021 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    25081 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    25141 cgcggttgat ggatatgagc catgatctga cattatccgg ttgggcgacg gacagtaacg
    25201 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    25261 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    25321 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    25381 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    25441 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    25501 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    25561 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    25621 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
    25681 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    25741 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    25801 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    25861 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    25921 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    25981 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    26041 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    26101 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    26161 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    26221 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    26281 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    26341 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    26401 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    26461 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    26521 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    26581 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    26641 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    26701 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    26761 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    26821 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    26881 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    26941 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    27001 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    27061 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    27121 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    27181 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    27241 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    27301 tggttaacct ccttggcgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    27361 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    27421 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    27481 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    27541 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    27601 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    27661 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    27721 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    27781 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    27841 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    27901 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    27961 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    28021 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    28081 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    28141 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    28201 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    28261 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    28321 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    28381 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    28441 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    28501 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    28561 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    28621 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    28681 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    28741 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    28801 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctaatattg cgcccaaaag
    28861 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    28921 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    28981 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    29041 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    29101 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    29161 ctgaatcctc cttccagatt atccacctca ttctgacaag agattaaatc ttgtcagaat
    29221 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    29281 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    29341 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    29401 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    29461 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    29521 gaggcggtgc cggagattat tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag
    29581 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    29641 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    29701 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    29761 gagagggtta gggtaagggg aaaagcgccc accctgccgc agcctgctcc ggtcggacct
    29821 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    29881 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    29941 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactatc
    30001 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    30061 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    30121 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    30181 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    30241 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    30301 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    30361 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    30421 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    30481 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcttccgg
    30541 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    30601 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    30661 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    30721 cgaattgaaa taatcattct gttggtaaat attgggcggc gttctgccgc ccgttatctc
    30781 tgttatacct ttctgatatt tgttatcgcc gatccgtctt tctccccttc ccgccttgcg
    30841 tcaggataac gatttccttt acgaccaagg agcgcccatg gaacaacgcc acatcaccgg
    30901 caaaagccac tggtatcatg aaacgcaatc cagtactgcg gagtatgacg ttctgcctct
    30961 ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga
    31021 aacgctggct cccttccttt catggctggt ccctgcgcgc gttcttgcag tggaattgtt
    31081 ccctgaccag cttaccgtga cccgttcaca gaccttcacc gcttatgaac gcttgtctac
    31141 ggccctgacg gttgctcagg tttgcggcgt ccagcggtta tgtaactact attcggcgcg
    31201 acttacgccg ctccccgggc ctgattcctc cagggaaagc aatcataggt tggcacaaat
    31261 cacgcaatat gcccgccaac tggctagctc gccttctatt atcgacaacc gatcgcgcca
    31321 gcatctgaat gacgtcggtc ttactgcctg ggactgtgtg atcattaacc aaatcattgg
    31381 ttttattggc tttcaggcgc ggaccattgc gacatttcag gcttatctcg ggcacccggt
    31441 acgctggtta cccggtctgg aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga
    31501 atcaatacgc tggcgaagca gctatgaggt ggaaaaacta cctgaagagt acacaaaaag
    31561 ttcaacagca gaactttgcc aactggccga aacactctct ctccacccta tttcactttc
    31621 ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata ataagcttgc
    31681 ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct gtatggattc
    31741 atcaaatgaa tataaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa
    31801 tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg gctgacccga
    31861 gcacccgatc gctttagcgc cgcccagttc agccctttac tcggacacga aaaatcatca
    31921 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    31981 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    32041 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    32101 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    32161 aaaaggcttt ggcttcctgg aagtcgacgc gaaaaaaagt tatttcattc cgccgccgca
    32221 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    32281 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    32341 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    32401 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    32461 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    32521 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    32581 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    32641 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    32701 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    32761 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    32821 caccaactat ctgcctggct tcaacatccc tatgcttcct cgcgagcttt ctgacgatct
    32881 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    32941 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    33001 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    33061 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    33121 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    33181 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    33241 agaagcgatg attgccgcta acatttgtgc ggcccgcgtt ctgcgcgata agctcggttt
    33301 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    33361 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    33421 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    33481 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    33541 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    33601 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    33661 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    33721 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    33781 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    33841 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    33901 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    33961 agtccgcatg gaaacccgcg gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    34021 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    34081 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    34141 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    34201 tttaaccgcg gactccgcag ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    34261 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    34321 cgatgttttg cgctttttta ccaccgaaac cacagaacct gatcgtactt tgcagttatc
    34381 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    34441 tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    34501 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    34561 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    34621 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca ctgggttgaa
    34681 agaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    34741 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    34801 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaaa taaatttgtc
    34861 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
    34921 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    34981 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc tgataacaat
    35041 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    35101 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    35161 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    35221 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
    35281 cgtattggtt taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa
    35341 catggttcgg acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag
    35401 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    35461 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    35521 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    35581 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
    35641 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
    35701 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
    35761 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    35821 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    35881 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    35941 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    36001 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    36061 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    36121 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    36181 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    36241 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
    36301 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
    36361 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
    36421 ttgtgttaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
    36481 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
    36541 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta
    36601 tttttttatt tagtattata acgttataag aattacagcg atgaattccc gacaacaaac
    36661 tattctacag atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac
    36721 tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct
    36781 ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat
    36841 gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg
    36901 cgaaaccatc tttatcgaaa atggcagcag caatgccctg ctcgcccgga ctctggggga
    36961 gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt tgctgaaaga
    37021 cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt
    37081 tggcccgttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat ttattggtat
    37141 tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt
    37201 caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg
    37261 tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc
    37321 gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt
    37381 tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct
    37441 aagatgattc ctggttgata attaagacta tttacctgtt attaacacgc ttaagatata
    37501 aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg
    37561 cgagcagatt tcacggaata atttcaccag gcttattctt agctattata gttatagaga
    37621 gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa
    37681 tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt
    37741 ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac
    37801 tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc
    37861 accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct
    37921 tgattctgtt cagtacgaaa taactgaata atacgcacag taaaatattc gaaagccacg
    37981 gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt
    38041 caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa
    38101 ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcggcat
    38161 catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac
    38221 gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa
    38281 tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg
    38341 ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg
    38401 gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg
    38461 gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc
    38521 ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag
    38581 gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat
    38641 gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag
    38701 cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc
    38761 accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg
    38821 cgctgcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg
    38881 ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag
    38941 acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata
    39001 atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa
    39061 cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc
    39121 gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt
    39181 ttctgctccg tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa
    39241 gtcggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca
    39301 ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca
    39361 gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct
    39421 ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac
    39481 acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg cctcactacc
    39541 gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg tgttctcttc
    39601 caccgcgcgt tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt
    39661 ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa
    39721 actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca tcatgatgga
    39781 tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc
    39841 gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac
    39901 gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca
    39961 ctcgctggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt
    40021 ttcatcggtc agctgattga acatgtcttc cgcgcggtcg tataacccgg cggccatgta
    40081 atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagtg aggcgctttc
    40141 cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt
    40201 tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag
    40261 gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg
    40321 cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc ccatatacca
    40381 gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc
    40441 cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc
    40501 cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag
    40561 gccacaaatc aaccaaccga tagcaacccc cgcagcaaac aataccgcca gcaatgtgga
    40621 aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc
    40681 acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt
    40741 cacattactt cccgttatgt ggttaaagcg aataaattat ggtcaactta ccgcaatagc
    40801 cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat
    40861 ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt
    40921 cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg
    40981 ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca
    41041 tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg
    41101 ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa
    41161 acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat
    41221 attgtgggat atttttctct tcagccaact gttctttcac tagatttccg cgttctgctc
    41281 gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa
    41341 aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaatga
    41401 ttacggccgc cagaatggca aataatacaa aggcagcctt aatgcaaaaa cgcagacacc
    41461 agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt
    41521 cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc
    41581 cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc
    41641 tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc
    41701 cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt
    41761 aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga
    41821 atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc
    41881 tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg
    41941 atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg
    42001 ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg
    42061 ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc
    42121 tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc
    42181 atcagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac
    42241 tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga
    42301 ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata
    42361 acgaacatta tctcgatacc aaagccgaga aaatgggtca tttgctgaac aaataaccct
    42421 ctcgcattgt ggaattaatt tgcttgccgg aagcaaaata accggcaagc aaatatttgt
    42481 tacttcagca tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac
    42541 tccgtcgcgg tgtcgatacg acaacggcag ggtacgacct cctggctacc atccgcgcgc
    42601 gtaagcgtca ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc aatatcaatc
    42661 ttctcttccc cggttagtcc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc
    42721 aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc
    42781 acacgaattc caagcagacg aggacctttt gccgcccagt cacggcttga gcctgaacca
    42841 tactctttcc cggcaatcac cgccagcggc gtttgttcct gcttatagcg catcgcagca
    42901 tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca
    42961 ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg
    43021 ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga
    43081 tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg
    43141 actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt
    43201 gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa
    43261 ccgtaggtat cggatcgtgt gacgttaatt tccttccact ctgctgtgcc ttcaaaaact
    43321 tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct
    43381 tgtgccgatg gccagatatc tttcagataa accggatcgc ctttacgatc atgaccaata
    43441 ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc
    43501 ggcgaggcca gccagttggt tttaaccagc ggatggatac ggccttcaaa gttacggttg
    43561 ccggacagca ctgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg
    43621 ggcagcggcc cagagttacc aatacaggta gtacaaccgt atcccacaag gttaaacccc
    43681 agttcgtcga gaaacggtgt cagtttcgct tttgccagat agtcagaaac aactttcgag
    43741 cctggtgcca gcgacgcttt gacccacggt tgccgcttga ggccaagaat tacggctttt
    43801 ttcgccagca agcctgcagc catcagcaca cttgggttag aggtgttggt gcacgaggtt
    43861 atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa
    43921 tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat
    43981 acttttggta catcgggcag tgcaacgcga tcctgtgggc gttttggccc tgccaggctc
    44041 gcttcaacgt cattcatatc cagttctaac gtactggtaa aaatcggttc atcgcccggg
    44101 ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcaacctg atcttcgctg
    44161 cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg
    44221 gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccagcgg tagtgaatcc
    44281 agaccatcac cataaaattc gacgaatttc tccaccacgc catgtttgcg cagcatttgg
    44341 gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaatttacc ggtaagttta
    44401 aagcccacta cgtccgggat aagcatggaa accggctggc ctaacattgc ggcttctgct
    44461 tcgatcccac ccacgcccca ccccagcacg ccaaggccgt tgatcatagt ggtgtgcgag
    44521 tcagtaccaa cgagtgtatc cggataagca atccattcac cgtcctgcaa ttcactccac
    44581 actgctttgc cgagatattc gaggttaacc tgatggcaaa tgcctgtgcc tggcggcacg
    44641 acgctaaacc gactgaacgc ttgctttccc catttcagga acacataacg ttcgtggttg
    44701 cgctccattt ccaggcgtac gttttcttca aatgcctcat catcaccaaa acgatcgacg
    44761 gtcaccgagt ggtcaatgac caggtcgacc ggtgagagcg ggttaacctt tgcagtatcg
    44821 ccgccgaggc gtttaaccgc ttcgcgcatt gccgccagat caacaacggc aggtacgccg
    44881 gtaaagtcct gcatcaacac ccttgccggg cggtaggcaa tttcacggtc agcatgggca
    44941 tttttcagcc atcctgccag cgcgtggata tcctcttcgg taaccgagtt accatcctgc
    45001 cagcgcagca ggttttcgag caaaactttg agtgacttgg gtagacgggt gatatcgccc
    45061 agtgatttag cagcaagcgg caggctgtag tagtggtaag ttttatcttt ggcctgcaac
    45121 gtgtccttac tggcttctcg tagggttgac gacatagctc ctccttaatg acagggttgc
    45181 gaagccctga ttctcttcag ggtgaatatt aaagataaca caaacatcgc gtaacgattt
    45241 gataacaacc caaattgata aaagaggaga aaccttgtgg caaagcaaat gacaaccccg
    45301 ccgcagcggg gtcaaggatc agtgagtaat gatccagagg agttcggccc agaagagcat
    45361 cgacagagaa aacgcgccca tccaggacca atatttcata tttgtgttga tcataggcta
    45421 cacccttaac ggttattatt ttgtgttgag ataactcaag ctgttattgc tgcggtgtat
    45481 taaaagccct ttaattcatt aaaaatgggt aataagaatg atgataatta ttgtttcggc
    45541 tgatcatctt cagcaaacag ttctataaag gcttgctgct gcgatgttag ttgccagtcg
    45601 tctggaataa cgacggaaca ttcccgtttc gtttctgttt cattgtcact atgctggcat
    45661 aatggtgacg actccagttg ctcctgaccg accatagtta tccccacact ttccaaatca
    45721 gcagtgcgac aaccacccag aaaagtgcag aaccaagaaa aaccgccagc caggctttac
    45781 gttttaattc aggatcgcgg cggggttcct gatttccaga aggcattgct aacctcatat
    45841 aatcgccatc acttatcagc aagacgttga acgattggtg taattaatca tacgatgaga
    45901 caaatcctaa agaaacttta gt
//
LOCUS       DAETQN010000017        43714 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.017, whole genome shotgun sequence.
ACCESSION   DAETQN010000017 DAETQN010000000
VERSION     DAETQN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43714
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.017"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..560)
                     /locus_tag="K0W68_001015"
     CDS             complement(<1..560)
                     /locus_tag="K0W68_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBI3072655.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(763..999)
                     /gene="feoC"
                     /locus_tag="K0W68_001016"
     CDS             complement(763..999)
                     /gene="feoC"
                     /locus_tag="K0W68_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBI3072656.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(999..3320)
                     /gene="feoB"
                     /locus_tag="K0W68_001017"
     CDS             complement(999..3320)
                     /gene="feoB"
                     /locus_tag="K0W68_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBI3072657.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3337..3564)
                     /gene="feoA"
                     /locus_tag="K0W68_001018"
     CDS             complement(3337..3564)
                     /gene="feoA"
                     /locus_tag="K0W68_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBI3072658.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4002..6323)
                     /locus_tag="K0W68_001019"
     CDS             complement(4002..6323)
                     /locus_tag="K0W68_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBI3072659.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6420..6896)
                     /gene="greB"
                     /locus_tag="K0W68_001020"
     CDS             complement(6420..6896)
                     /gene="greB"
                     /locus_tag="K0W68_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBI3072660.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7124..7843
                     /gene="ompR"
                     /locus_tag="K0W68_001021"
     CDS             7124..7843
                     /gene="ompR"
                     /locus_tag="K0W68_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBI3072661.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7840..9192
                     /gene="envZ"
                     /locus_tag="K0W68_001022"
     CDS             7840..9192
                     /gene="envZ"
                     /locus_tag="K0W68_001022"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBI3072662.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9268..10890)
                     /gene="pckA"
                     /locus_tag="K0W68_001023"
     CDS             complement(9268..10890)
                     /gene="pckA"
                     /locus_tag="K0W68_001023"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBI3072663.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11269..11583
                     /locus_tag="K0W68_001024"
     CDS             11269..11583
                     /locus_tag="K0W68_001024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBI3072664.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FG"
     gene            11596..12969
                     /locus_tag="K0W68_001025"
     CDS             11596..12969
                     /locus_tag="K0W68_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBI3072665.1"
                     /translation="MAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNG
                     LTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALVVILARTQSRLIDSIQ
                     KLFTLIATGLLPLVSLLTLMFIITLPFTDLSAISRHISAAGLLLTLAFLQLILMAIVR
                     DPQKASLPWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVL
                     LVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMA
                     RYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQ
                     EQVSEKVLAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQA
                     ERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLDLTVDGET
                     LEINLSK"
     gene            complement(13104..13982)
                     /gene="hslO"
                     /locus_tag="K0W68_001026"
     CDS             complement(13104..13982)
                     /gene="hslO"
                     /locus_tag="K0W68_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBI3072666.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14007..14408)
                     /gene="hslR"
                     /locus_tag="K0W68_001027"
     CDS             complement(14007..14408)
                     /gene="hslR"
                     /locus_tag="K0W68_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBI3072667.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14419..15087)
                     /gene="yrfG"
                     /locus_tag="K0W68_001028"
     CDS             complement(14419..15087)
                     /gene="yrfG"
                     /locus_tag="K0W68_001028"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBI3072668.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15152..17287)
                     /gene="igaA"
                     /locus_tag="K0W68_001029"
     CDS             complement(15152..17287)
                     /gene="igaA"
                     /locus_tag="K0W68_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBI3072669.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17607..18167
                     /gene="nudE"
                     /locus_tag="K0W68_001030"
     CDS             17607..18167
                     /gene="nudE"
                     /locus_tag="K0W68_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBI3072670.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18332..20884)
                     /gene="mrcA"
                     /locus_tag="K0W68_001031"
     CDS             complement(18332..20884)
                     /gene="mrcA"
                     /locus_tag="K0W68_001031"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBI3072671.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21004..21783
                     /gene="hofM"
                     /locus_tag="K0W68_001032"
     CDS             21004..21783
                     /gene="hofM"
                     /locus_tag="K0W68_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBI3072672.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21783..22322
                     /gene="hofN"
                     /locus_tag="K0W68_001033"
                     /pseudo
     CDS             21783..22322
                     /gene="hofN"
                     /locus_tag="K0W68_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            22306..22746
                     /gene="hofO"
                     /locus_tag="K0W68_001034"
     CDS             22306..22746
                     /gene="hofO"
                     /locus_tag="K0W68_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBI3072673.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22736..23140
                     /gene="hofP"
                     /locus_tag="K0W68_001035"
     CDS             22736..23140
                     /gene="hofP"
                     /locus_tag="K0W68_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBI3072674.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23052..24290
                     /gene="hofQ"
                     /locus_tag="K0W68_001036"
     CDS             23052..24290
                     /gene="hofQ"
                     /locus_tag="K0W68_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBI3072675.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24691..25212
                     /gene="aroK"
                     /locus_tag="K0W68_001037"
     CDS             24691..25212
                     /gene="aroK"
                     /locus_tag="K0W68_001037"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBI3072676.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25269..26357
                     /gene="aroB"
                     /locus_tag="K0W68_001038"
     CDS             25269..26357
                     /gene="aroB"
                     /locus_tag="K0W68_001038"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBI3072677.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26449..27735
                     /gene="damX"
                     /locus_tag="K0W68_001039"
     CDS             26449..27735
                     /gene="damX"
                     /locus_tag="K0W68_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBI3072678.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27842..28678
                     /gene="dam"
                     /locus_tag="K0W68_001040"
     CDS             27842..28678
                     /gene="dam"
                     /locus_tag="K0W68_001040"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBI3072679.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28696..29373
                     /gene="rpe"
                     /locus_tag="K0W68_001041"
     CDS             28696..29373
                     /gene="rpe"
                     /locus_tag="K0W68_001041"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBI3072680.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29366..30124
                     /gene="gph"
                     /locus_tag="K0W68_001042"
     CDS             29366..30124
                     /gene="gph"
                     /locus_tag="K0W68_001042"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBI3072681.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30117..31121
                     /gene="trpS"
                     /locus_tag="K0W68_001043"
     CDS             30117..31121
                     /gene="trpS"
                     /locus_tag="K0W68_001043"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBI3072682.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31281..32186
                     /locus_tag="K0W68_001044"
     CDS             31281..32186
                     /locus_tag="K0W68_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072683.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32203..32565
                     /locus_tag="K0W68_001045"
     CDS             32203..32565
                     /locus_tag="K0W68_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBI3072684.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32649..33812
                     /locus_tag="K0W68_001046"
                     /pseudo
     CDS             32649..33812
                     /locus_tag="K0W68_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33812..35038
                     /locus_tag="K0W68_001047"
                     /pseudo
     CDS             33812..35038
                     /locus_tag="K0W68_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35035..35913
                     /locus_tag="K0W68_001048"
     CDS             35035..35913
                     /locus_tag="K0W68_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBI3072685.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35924..36277
                     /locus_tag="K0W68_001049"
     CDS             35924..36277
                     /locus_tag="K0W68_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBI3072686.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36289..37593
                     /locus_tag="K0W68_001050"
     CDS             36289..37593
                     /locus_tag="K0W68_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBI3072687.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37605..38690
                     /locus_tag="K0W68_001051"
     CDS             37605..38690
                     /locus_tag="K0W68_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBI3072688.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38842..39573)
                     /gene="phoB"
                     /locus_tag="K0W68_001052"
     CDS             complement(38842..39573)
                     /gene="phoB"
                     /locus_tag="K0W68_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBI3072689.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39673..40458)
                     /gene="frlD"
                     /locus_tag="K0W68_001053"
     CDS             complement(39673..40458)
                     /gene="frlD"
                     /locus_tag="K0W68_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBI3072690.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40455..41286)
                     /gene="frlC"
                     /locus_tag="K0W68_001054"
                     /pseudo
     CDS             complement(40455..41286)
                     /gene="frlC"
                     /locus_tag="K0W68_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41336..42358)
                     /gene="frlB"
                     /locus_tag="K0W68_001055"
     CDS             complement(41336..42358)
                     /gene="frlB"
                     /locus_tag="K0W68_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBI3072691.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTEC
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42379..>43714)
                     /gene="frlA"
                     /locus_tag="K0W68_001056"
     CDS             complement(42379..>43714)
                     /gene="frlA"
                     /locus_tag="K0W68_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HBI3072692.1"
                     /translation="GSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWLT
                     VLAFVIGELIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDAP
                     SLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKI
                     IPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMTG
                     EIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPALG
                     STAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSII
                     LQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLM
                     TTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT        10157 a        11126 c        11988 g        10443 t
ORIGIN      
        1 tcgcgttgac gaatatgttt ttgaattaac tccagcagcg ccatacgtcg gtgctgcatg
       61 atttcatcat caggcatgac ggtgacatca atcagcggaa atgcggaggc ataaagctgc
      121 cttgccagtt tgggatcggc gaaacaatcc agccaacaca gcgaataggg ataggggctt
      181 tcaataccgt ggtaaaacaa caatagcacc accatcggca acgttttgta tccagcatca
      241 aggtgatttt gcattgcggc aatagcgtaa cgcatcatgc gaaatgcgat cagtttgttt
      301 gaggtgcttt gatgttcaat cagacaatag atgtatcctg gtccttgttc cgttttcacc
      361 gaccacagca catcggaata gctttcacgc agatcgtcat caataaagct gctcgactcc
      421 agcttgaggg ttttcatatc acaaagcgcg tgaatgggtg ccggtaaatg aaacgcaaga
      481 aaatcacgag ccgtgtccgg ttggcgtaaa aagagtttga acagcgcatc gtgtggggtg
      541 gaactctgct ttttgctcat ggcgttcctt cacctgttgt ttgaatgccg cgactctacg
      601 cgccactcca cacctgtgca ttagcgagtt ttcatccctg cgagatgctt tcaaaggaac
      661 ttacgccgtg ctgcaagagt tgcattttta tggaaagccg cacgcaaaaa agcccggatg
      721 cagacgcata ccgggctttc caaaacaggc gatggagtaa ggttaacgca gcgcccacca
      781 ctcgcgcaga caggcttttc cttccgggca gcttttacaa ctgccagaga ggcagccgtc
      841 aggttcttcc tgaatccgca cggctttgcc catactttcc agttgttgca gcatggcgtt
      901 aatcattggc tgcggagtgt tcaatgtctg gcttatctgg gtcgcttcca tacggccccg
      961 taacgccagc aaatcacgta cctgaataag tgaagccatt aatggcaatc accggtggtg
     1021 ctggctgcgc agcaactgct taccgacttg cgggttgcca gcagttcgat atccacccgg
     1081 ctacgcgcgc ggcgcagcag accgataact acgatgttaa acaggattac cgccagaatg
     1141 cacaccaggc tgtaagttgg gtgctgactg tagctggcga cctgatagaa caatgttgcc
     1201 agtgagtaag cgatattcag cccccacagg atggagaagc ccatccagcc acggcttgat
     1261 tcacgggcga tggctcccat caccgagata catggtacat acagcaggac gaaaatcagg
     1321 tagctgtatg cggcagctgc gctaccgaat ttctgatcca tcacgcccat cgccccggta
     1381 cccatttcac cgtcgccttt gctagcttca atggggttca tcagtacgct aaggctgaag
     1441 gtgtctttca ggctctgcca ggtttcatct accgcactga acagctcttc accgaggttg
     1501 aattccgccg gattgaactc ttcgtcctga atattttctg cggtgtagag ggtgttgagc
     1561 gtacccacca ccacttcttt tgccattgcg cccgtaaaca ggccaaccgt tgcctgccag
     1621 ttatcttcat gtacgccaat cggcttgaag acaggggtta tcacccggct gacggacgcc
     1681 agcgccgagt cgttgatgtt atcgacgatt ttcccgctca acgagaagct gttgaaagcg
     1741 ctcaggaaaa tgctgacgat gataatcact ttaccagcac gcagaacgaa gcccttcaga
     1801 cgctgccagg tctggataat caggctttta acgtgtggta catggtagac cggcagctcc
     1861 atgacaaacg gcgtcgcttc accgcgcatg atggtgtact tgagcatcag gccagtcagc
     1921 accgccatca cgataccgag catatacagc gagaaaaccg ccagggcacc gttctgcccg
     1981 aagaaggcag ccgcgaatac tgcgaagata gccagacgcg cgccgcagga cataaacggt
     2041 gccatcatga tggtcatcag acgttcacgc ggtgcatcaa gcgtacgtgc gcccattacc
     2101 gacggtacgt tacaaccgaa accgacgatc agcggcacaa aggatttccc cggcaagccc
     2161 agcgcctgca tcaggcggtc catcacaaac gctgcacgcg ccatatagcc ggagtcctca
     2221 aggaaggaga ggaacaggta catcatgccg atctgcggca ccagtggcag cacggtgtta
     2281 ataccgccac ccaggccctg ggcgaggaag atagtcagcc agtccgggaa gtggagcgtg
     2341 tagccaatcc attgaatacc atgcacaaat agcgccacgg agccgacgtc aaacagcggc
     2401 tgtaacgccc cgccgatgtt gatagccagc aggaacatca ggtacatcac aaagaggaaa
     2461 atcggcagac cgaggaaacg gttgagcacg attttatcta ccgcagtggt gaaacggctg
     2521 ggttctgccg tcagggtgtt gcttaccaca tcacagatgg cagctatgca ctggtaacgc
     2581 gcatcggcaa tgtgcagcgc cggatcatcc atttcattac gcagacgggc aagggcggca
     2641 tccagatgct gcgacgcttc accggcgtag gcgcggctgt agatatcgcc ttccagcatt
     2701 tgcaggccca gccagcgacg ttgtttcagc gggatgtcgg aaggcatcac ttttgccagt
     2761 gaatctgctt cgttgagtag cggctgtgcg taatgcacca gttccacatt ctcgttagct
     2821 ttatagcgat caatcgccag cttgagcgct tcaataccgc gaccacgggt tgaaaccagc
     2881 gggatcactg ggcagccaag acgcgccgac agagcatcaa tttcaatacg aatattttgc
     2941 ttctcggcaa tgtcgagcat gttcagtgcc acgatgcagg gaatgccgag ttccagcagt
     3001 tgtagcgtca ggtacaggtt acgctcaagg ttagacgcat ccaccacgtt aatcagcagg
     3061 tcggcgtcgc cactcaaaat gtagtgacag gcgatttgct catcgagcga ggtctgcgat
     3121 gagatggtgg tcagagaata ggtgccgggc aggtccacca gcgtgacctg atgatcggtg
     3181 gtggagaatt gcccttcttt acgttcgacg gtaacgccag cccagttacc tacacgctga
     3241 cgtgcgctag tgagctggtt aaataaggtt gtcttgccag aatttggatt accaattaag
     3301 ccaatggtta attttttcat tgttgttatc accgtattaa caggaaaccg cttccacttc
     3361 taataaggcc agatcttttt tgcgtaatac caggctcaca cgacgggttt cgatatgaat
     3421 ggggtcgccg agtggagcga cgcgcaccac attaaaagag gagccaggta acatgccaag
     3481 agaaagcagt ttttggcgat atgccgggct gatttcacgg gaaaagccag tgattttcca
     3541 cgcagtatct ggagtgtatt gcataggtgc ctacttgttt ctcattaact ggataactac
     3601 ctcatggggc gtttgacagc gacatgagac cagatgattt gccaacaaag gagagattaa
     3661 ataatcaaca acacaatgat aatgagaatg gtttctatca gcaatactta aaatgtgtgc
     3721 gaatgttggt ttgaataata atctgactca atgttgatgt ggctcaaggt tatatcgcag
     3781 cgaaaatata attcgaatag tgaattaccc ggactggcta atgtttaatt aaggtttcag
     3841 ttgttaatga agtgataaat attgccggaa tatattatga gtttggttgt ggaataaaaa
     3901 ggccggagat attctccggc acttttatgt caggcaaggc gttcgttgcc agatgcggcg
     3961 tgaacgcctt atcgcgcagg tttaggggcc agtccgggcg tttaacgttt tttgcccatt
     4021 gccgccgcca gcgcatccat catcgcgcta ttaccggcag gctgcgcttc acgaccgcgt
     4081 ggtttggctg ccgggcggtt gttttgcggg cgttcattac cgccgccgcg acgggcgttg
     4141 gtttcgccag gctgctcgtc cagacgcatg gtcagggcga tacgtttacg ctgaagatcc
     4201 acttccagca ctttcacctt cacaatgtcg cccgctttca ccacggtatg cggatcttcc
     4261 acaaacttgt tcgacaatga agagatgtga accaggccgt cctgatgcac gccaatatcg
     4321 acaaacgcgc caaagttggt gacgttggtg acagcgcctt cgaggatcat acccggttgc
     4381 aggtcgttca ttgtctcgac gccatcggca aactgagcgg ttttaaattc cggacgcgga
     4441 tcgcgacccg gtttttccag ctctttgatg atgtcagtta ctgtcggcac accgaatttt
     4501 tcatcagtaa agtcagacgc tttcaggtta cgcagttcgc tgctgttacc catcagatct
     4561 ttcagtgcct gctgtgttgc tgccagaatg cgttccacca ccggataggc ttccgggtga
     4621 acggtagagg cgtccagcgg gttatcaccg tggttaatgc gcaggaagcc cgcgcactgc
     4681 tcgaaggctt tcggccccag acggctgact ttcagcagtt gctgacggtt cttgaactgg
     4741 ccgttctcat cgcgccaggc cacgatgttt tgcgccatca tgcgcgtcag gcccgccacg
     4801 cgagttaata gcggaacaga agcggtgttg agatcgacgc caacggcgtt tacgcagtct
     4861 tctactactg catccagttt gcgggccagt tgcgtctggc tgacgtcatg ctgatactga
     4921 cctacgccga tagatttcgg atcgattttc accagctccg ccagcggatc ctgcaaacgg
     4981 cgggcgatag ataccgcacc acgcagcgaa acgtcgagat ccgggaactc ctgcgctgcc
     5041 agctcggaag ctgagtaaac cgacgcgcca gcttcgctga cgatcacttt ctgtgcggtc
     5101 actttcggga actgcttctg cacgtcgaga tagaaacgtt cagtttcgcg ggaagctgta
     5161 ccgttgccga tcgccaccag ttcaacgtta tgtttttcac acaaggcagc aacggtcatc
     5221 gctacttttg cggcttgtcc ggtgtgcggg taaatggtat cggtcgccac cagtttgcca
     5281 gtggcatcga ccactgccac ttttaccccg gtacgcagac ccggatcgag gcccatcgtt
     5341 gcacgcagtc cggcaggggc cgccatcagc agatcgtgca ggttacgggc aaagacgttg
     5401 attgcttcat cttccgcgcg ttcgcgcacg gtacccatca gttcggtttc cagatgcatc
     5461 agcaccttga tgcgccaggt ccagctcact acgcctttgc gccagctatc cgccggggca
     5521 ttgttcaggc gcaggccaag gtgatccatg atgatttgct cgcaatagct ctctttgggc
     5581 ggctcatcga actgcggatc ggcattcagc gaaagctgga gtacgccttc gttacgccca
     5641 cggaacatcg ccagcgcgcg gtgagaaggc accgtggaca acggttcgtg atgatcgaaa
     5701 tagtcgcgga atttcgcccc ttcctcttct ttaccgctca ccaccgtaga aaccaaatgc
     5761 gcgttcttcc acagataatc acgcactttc gccagcagcg cggcatcttc ggcaaaccgt
     5821 tccatcagga tatagcgcgc gccgtccagc gcggctttgg tatctgccac gcctttatcg
     5881 gcatcaatat attgcgcagc ggcgacttct ggtgtgtgtg acggatcgct ccacagcagg
     5941 tcagccaacg gctcaagccc tgcttcaatg gcgatttgcc cgcgggtgcg gcgtttaggt
     6001 ttgtagggca ggtagaggtc ttcgagttcg gttttgctta gggtggcgtt gatggccttc
     6061 gccagatcat cggtgagttt gccttgctcg gaaatggact tgaggatcgc ctgacgtctc
     6121 tcttccagct cgcgcagata gctcagacgc gtttccagat tacgcagctg cgtgtcatcc
     6181 agaccgccgg tgatttcctt acgataacgt gcgataaacg gcacggtatt cccttcgtca
     6241 agcaggcgaa cggcagcgtc aacctgttcc gggcgcgcct gaatttcacc cgcaataatg
     6301 cggcagaacg aatcattcat catggtttga gttcatcttt tcggatcaaa aatcagggga
     6361 tagttatacg gactggctgg caaaaatgcc agccatcggc aggaggttaa gactcttcat
     6421 tacggtttca cgtattcgat agcattaaca taccagctcg cttccccggc aggggtattc
     6481 accaccgcca gatcgccgac ttcttttttc agcaatgcgc gggccatcgg ggaatcgata
     6541 gagatgtaat ctttacggcc aaaaatttca tcgtagccga caatacggaa acggtgagtc
     6601 acgccatcgt cgttttcaat ctccacccac gcgccaaaaa agactttgcc ttcctgctga
     6661 ggggagtaat tgacgatttt gagattttcc atgcatttag tgagatagcg cacgcgacgg
     6721 tcgatttcac gcagacgctt tttattatac tgatagtcag cattttcgct gcggtcgccc
     6781 agacttgcgg cccaggtcac cttttttgtg acctccgggc gttcttcacg ccagagataa
     6841 ttaagctctt gtttgagttt ttcataccct tcccgggtaa ccaggggcgt tttcatctcg
     6901 ttgattccct ttgtctgttt gataatgcgc acattgggta taacgtgatc atatcaacag
     6961 aatcaataat gtttcgccga ataaattgta tacttaagct gctgtttaat atgctttgta
     7021 acaatttagg ctgaaattca taccagattt agctggtgac gaacgtgagc ttttttaaga
     7081 atacacgctt acaaattgtt gcgaaccttt gggagtacaa acaatgcaag agaactacaa
     7141 gattctggtg gtcgatgacg acatgcgcct gcgtgcgctg ctggaacgtt atctcaccga
     7201 acaaggcttc caggttcgaa gcgtcgctaa tgcagaacag atggatcgcc tgctgactcg
     7261 tgaatctttc catcttatgg tactggattt aatgttacct ggtgaagatg gcttgtcgat
     7321 ttgccgacgt cttcgtagtc agagcaaccc gatgccgatc attatggtga cggcgaaagg
     7381 ggaagaagtg gaccgtatcg taggcctgga gattggcgct gacgactaca ttccaaaacc
     7441 gtttaacccg cgtgaactgc tggcccgtat tcgtgcggtg ctgcgtcgtc aggcgaacga
     7501 actgccaggc gcaccgtcac aggaagaggc ggtaattgct ttcggtaagt tcaaacttaa
     7561 cctcggtacg cgcgaaatgt tccgcgaaga tgagccgatg ccgctcacca gcggtgagtt
     7621 tgcggtactg aaggcactgg tcagccatcc gcgtgagccg ctctcccgcg ataagctgat
     7681 gaaccttgcc cgtggtcgtg aatattccgc aatggaacgc tccatcgacg tgcagatttc
     7741 gcgtctgcgc cgcatggtag aagaagatcc agcgcatccg cgttacattc agaccgtttg
     7801 gggcctgggc tacgtctttg taccggacgg ctctaaagca tgaggcgatt gcgcttctcg
     7861 ccacgaagtt catttgcccg tacgttattg ctcatcgtca ccttgctgtt cgccagcctg
     7921 gtgacgactt atctggtggt gctgaacttc gcgattttgc cgagcctcca gcagtttaat
     7981 aaagtcctcg cgtacgaagt gcgtatgttg atgaccgaca aactgcaact ggaggacggc
     8041 acgcagttgg ttgtgcctcc cgctttccgt cgggagatct accgtgagct ggggatctct
     8101 ctctactcca acgaggctgc cgaagaggca ggtctgcgtt gggcgcaaca ctatgaattc
     8161 ttaagccatc agatggcaca gcaactgggc ggcccgacgg aagtgcgcgt tgaggtcaac
     8221 aaaagttcgc ctgtcgtctg gctgaaaacc tggctgtcgc ccaatatctg ggtacgcgtg
     8281 ccgctgaccg aaattcatca gggcgatttc tctccgctgt tccgctatac gctggcgatt
     8341 atgctattgg cgataggcgg ggcgtggctg tttattcgta tccagaaccg accgttggtc
     8401 gatctcgaac atgctgcttt gcaggtcggt aaagggatta ttccgccgcc gctgcgtgag
     8461 tatggcgcgt ccgaggtgcg ttccgttacc cgcgccttta accatatggc ggctggtgtt
     8521 aagcaactgg cggatgaccg cacgctgctg atggcggggg taagtcacga cttgcgcacg
     8581 ccgctgacgc gtattcgcct ggcgactgag atgatgagcg agcaggatgg ctatctggca
     8641 gaatcgatca ataaagatat cgaagagtgc aacgccatca ttgagcagtt tatcgactac
     8701 ctgcgcaccg ggcaggagat gccgatggaa atggcggatc ttaatgcagt gctcggtgag
     8761 gtgattgctg ccgaaagtgg ctatgagcgg gaaattgaaa ccgcgcttta ccccggcagc
     8821 attgaagtga aaatgcaccc gctgtcgatc aaacgcgcgg tggcgaatat ggtggtcaac
     8881 gccgctcgtt atggcaatgg ctggatcaaa gtcagcagcg gaacggagcc gaatcgcgcc
     8941 tggttccagg tggaagatga cggtccggga attgcgccgg aacaacgtaa gcacctgttc
     9001 cagccgtttg tccgcggcga cagtgcgcgc accattagcg gcacgggatt agggctggca
     9061 attgtgcagc gtatcgtgga taaccataac gggatgctgg agcttggcac cagcgagcgg
     9121 ggcgggcttt ccattcgcgc ctggctgcca gtgccggtaa cgcgggcgca gggcacgaca
     9181 aaagaagggt aaataaacgg gaggcgaagg tgcctcccgt tttgctttct ataagatact
     9241 ggatagatat tctccagctt caaatcatta cagtttcgga ccagccgcta ccagcgcggc
     9301 acccgcaggg gtgtcggtgt atttatcgaa gttgtcgata aacagtttcg ccagggtttc
     9361 ggctttttcc tgccactgtt ccggagaagc gtaggtgtta cgcggatcga gaatcttcgt
     9421 gtctacgccc ggcagttcgg ttgggatcgc caggttaaac atcggcagag tgaaggtttc
     9481 tgcattatcc agcgaaccgt tgaggatggc gtcgataatg gcgcgggtat ctttaatcga
     9541 gatacgtttg ccagtgccgt tccagccagt gttaaccaga taagcctgcg cgcccgccgc
     9601 ctgcatacgt ttcaccagca cttctgcgta ctgagtcggg tgcagcgaca ggaacgccgc
     9661 gccgaagcaa gcggagaagg ttggcgtcgg ttcggtgatg ccacgctcag taccggccag
     9721 tttggcggtg aagccagaga ggaagtgata ctgggtttga tcggcagtca ggcgagaaac
     9781 cggcggcaac acgccgaaag catcagcagt caggaagata accttagtcg cgtggcccgc
     9841 tttggaaacc ggcttaacga tgttatcgat gtgatagatt ggataagaaa cgcgggtgtt
     9901 ctcggttttt gaaccatcat caaagtcgat agtgccatct tcacgcacgg tgacgttttc
     9961 cagcagcgca tcacgacgga tagcgttgta gatttcaggt tccgcttctt tcgacagctt
    10021 gatagttttt gcgtagcagc cgccttcgaa attaaacacg ccgtcatcgt cccagccgtg
    10081 ttcgtcatcg ccaatcaggc ggcgtttcgg gtcggtggaa agggtggttt taccggtgcc
    10141 ggaaaggccg aagaacaccg caacatcgcc tttctcgcca acgttggcgg agcagtgcat
    10201 agaagcgata cctttcagcg gcagcaggta gttcatcatc gagaacatcc ctttcttcat
    10261 ttcgccgccg taccaggtgc cgccaatcag ctgcatgcgc tcggtcaggt taaacgccac
    10321 gaagttttcg gagttcagac cctgttcttt ccactgcggg ttagtgcact tcgcgccgtt
    10381 cataacgata aagtctggtt tgaaacctgc cagctcttca tcgctcgggc gaataaacat
    10441 gtttttgaca aaatgcgcct gccaggccac ttcggtgatg aaacggacgg aaagacgagt
    10501 atccgggttc gcaccacaga aagcgtcgac aacgaacaga cgtttgccgg aaagctgcct
    10561 ggtcaccagg cctttcagat gctgccaggt ttccggagag agaggtttgt tgtcgttctt
    10621 acctttgcct ttgtctgccc accagaaagt atcgcgagtg gtatcgtcac ggacgatata
    10681 cttatctttt ggtgaacgac cggtgaagat cccggtatcg acggcaacgg cacccagatt
    10741 agttaacacc ccgcgctcat aacctgtcag gctcggatcg agctcttcct gatacagcag
    10801 gtcgtagctt gggttgtaaa cgatatcatg tacgtcactg ataccataag cctcgagttc
    10861 ttgcggggtc aaaccattgt taacgcgcat ttcactgctc cttagccaat atgtattgcc
    10921 tgaatagtaa agtctttttg ggggtgttaa ccgcgacaag gctcatagat ttacgtatct
    10981 ggagaaattc atatttgaca aaaaactccg tgattcctgt cacgaaacgg ttgctattat
    11041 cgcagaaata gcattttagg tgtggaaaat gttctcaaaa ggttaaatct tggtttatat
    11101 ggcgggaata ttgtgagtgg aatcgcattc ctgaatgaaa ttgatgcaaa ttcaaccggt
    11161 aaagtatcga ttcaccacgc taactttttt tgattatttt ctgaaaaaag caaaatacta
    11221 ctgcccggat gcgataatta acccaccttt tgattctgga taaccaacat ggataacgtt
    11281 gaactttcac cggcaacacg ctgggggatg attgctaccg gattacttca ggggctggtt
    11341 tgctacctgc ttatcgcctg gcttgccgga aaaaatcaca gttggattgt ttatggcgtg
    11401 cctgcgaccg tcgcgttttc atccgtttta ctcttctccg tgatctcttt taaacagaag
    11461 cgcctttggg gatggctggc gctggtgttt attgccacga cagggatgag cggttggctg
    11521 aagtggcaaa ctgatggcat gaacccctgg agagctgaaa aggcgatttg ggattttggc
    11581 tgatatctgc tgttgatggc aatgttgttg ctaccgtgga tacaacaaag ccttcgtatc
    11641 cgcaatggca gtagccgcta tagctacttt taccagtcag tatggcataa cgtacttata
    11701 ttactggtga tttttctctc caatggcctg acgtggctgg tgcttttact gtggggtgag
    11761 ttgtttaaac ttgttggcat cacgtttttc aatacgctct tttttgcaac cgactggttt
    11821 atgtatctta catttggtct ggttaccgca ctggtggtga tcctcgcgcg tacacagtca
    11881 cgtttaatcg actctattca aaagttgttc acgctaatcg ccacggggtt gctgccgtta
    11941 gtatcattgc taaccctgat gtttatcatc accctgccgt ttacggacct gagcgcgatt
    12001 tctcgccaca tctccgccgc cgggttgctg ttaacgctgg cctttttgca attgatctta
    12061 atggctattg tccgcgatcc gcaaaaagcg tcacttccct ggacggggcc gttgcgttgc
    12121 ctgattaaaa ccgctttgct ggttgctccg ctgtatgtgt tcgtcgccgc ctgggcgtta
    12181 tggctgcggg tcgctcagta cggctggact gtcgaccgct tgcagggcgc gctggcggtg
    12241 ctggtgttac tggtgtggtc gctggggtat tttgtcagta tcgtctggcg taatgggcaa
    12301 aatccccttg ttcttcaggg caaagtgaac cttgcggttt cgttattggt gttggtgata
    12361 ctggtgcttc ttaattcgcc ggtgctggac agtatgcgca ttagcgtgaa cagtcatatg
    12421 gcgcgttatc agagcggcaa aaacacgcca gaccaggtaa ctatctacat gctcgaacag
    12481 agcggtcgct atggacgtgc ggcgcttgag tcgctgaaaa gcgatgccgg gtttatgaaa
    12541 gacccgaaac gcgcgcggga tctgctgatg gcgttagatg gagagcaaca tcttcaggaa
    12601 caggtatcgg aaaaagtatt agccgaaaat gtgttaattg ctcctggttc tgttaaaccg
    12661 gatgcgacat tctggtcggc cttaatccag gatcgctata acgtgatgac ctgtattgaa
    12721 aaagacgcct gcgtcctggt cgagcaagat ctgaatagtg atggtcaggc ggagcggatc
    12781 ctgtttgctt ttaatgatga cagagtcatt gtctatggct ttgactcaga cagaaaagaa
    12841 tgggacgcgc ttgatatgag tttacttccg aacgaaataa cgaaagaaaa attactcaca
    12901 gctgcgaagg atggaaaact ggatctgacg gtagatggtg aaacgctgga aataaactta
    12961 agtaagtgac aactctttta acggatacga cgctgtatct gaccaacaat aatttgcgat
    13021 ttatttcaca ggcctggtaa gcgcagcaca tcaggccatt acgtcgtaaa atccggcaga
    13081 gccctctctg ccggacatac tcattaatga acttgcggat ctgccggaga cgcgttgttg
    13141 cggatttcag caatatccat cgcattgaac agatagtggt taccgcagta atcacaatgc
    13201 atgtcaattt cgccatcttc cgccaggatg ctatcgactt cttcatcagg cagcgttttc
    13261 agcgcgtcgg cgcaacgttc acgcgagcag gtgcatttga actccacatc ctgcggatcg
    13321 taaaccgtca cctcttcttc gtgatacaaa cgccacaaca cttcgtttgc cggtaaggtc
    13381 agcagttctt cggttttgat ggtttcggtt agcgtcgcca ggtggtcaaa gtcgtcctgc
    13441 tgggcatttt gcgcaggcat tacctgcaac aacataccgc ctgcagccgg tttgccgtct
    13501 acgtcgccgg tgcgaataaa caggcgcgtc ggcagctgtt cagaacgcat aaagtaatct
    13561 tccaggcagg ccgccagggt atcaccttcc agaccaacca cgccctgata gcgttcgcct
    13621 tcgctcgggg taatggtgat caccacgtaa ccattgccga ccagcgtttt caggtcggca
    13681 ttttctggaa tttcgccctg cacgcgcgcc acaccgcgca tttgctggtt attgttaccg
    13741 ttaataaccg ccagattcat cggaccgtcg ccttgcagct gtacggtgat atcaccatca
    13801 aacttcagcg tagcggttaa caggctggtc gcaaccagca gttctgccag cacgttttta
    13861 acgggctgcg gataatcgtg attctcaagg atctgttgca gggtttccga aacggttacc
    13921 agttcgccgc gcacggcaaa gttttcaaac agatagcgat gtaattggtc atgttgcggc
    13981 ataatcatct ctcttgcagg tgacagttat tcactgtcgc cgtgtttaaa tcgtaacagg
    14041 tcgcggcgct cttttttgtc cgggcgtcgg tccgggtgcg gcatggttaa ggcattaagt
    14101 ttacgtgcca gcgccatttt ttcgcgtttc tctacacttt ccgcagtctc ttcatacagc
    14161 aaggctgcct cgctggcggg gcgacgctgt tcagtaatcg cctttacaat caccgtgcgt
    14221 tcgtcatttc cctggcgcag agtgagcgtg gcattcagct cgacgatttt gctcggcttg
    14281 ctgcgctgcc cgttgtaatg caccttaccg ccttcaatca tttcacgggc cagcgcgcgg
    14341 gttttataaa aacgggcagc ccatagccat ttatccagtc gaacctcaac agcaggtttt
    14401 tctttcatgg cgtctccttc acattagcga ggggatcagg cggcggtagt cattcagtga
    14461 cggatggcgt tgatactgtt tctcggcaat cccggaatca ggattagtca cgccgaggca
    14521 gtaacgaata ccaaattgcg cggcagcatc gagaatcgct tcgctgtcat caataaacag
    14581 cgttctttca gctttcagac ccgtagcttc ggccaccgca tgccataacc gctgatcctc
    14641 tttcggataa ccaaatgtgt gggtggaaag taataaatca aggtgtgcgt ccagaccggt
    14701 atgctcaagt tttaccgcca ggttgtgcgg atgcgcattg gtgagcaaaa ttcgctgctt
    14761 accgctggct ttcagtgcct caagaaacgg aatggtatct tcacgcagta cggcacgcgg
    14821 tcccatctcg gtggtcatcg cacagatatc cagacccagt tgctcactcc agtaatcaag
    14881 acagtaccag tttagcgtat gctgtacgtc gtgatattgc tggcgcatat attccatcgc
    14941 ttcctgtggc gtaaccccgt ttttcgcgcc ccatgtttca ggcaccagct tttgccagaa
    15001 atagttatcg aaggcgaggt cgagcaacgt gccgtccata tccagcagaa cggtatctac
    15061 gtcctgccag gcaatgttga tatgcatgag ggaaatctcc agagtgaagc aatttgcgcg
    15121 acagggtagc ataacctgcc gcgcaaacgt gttattcgat aaggctttct gaaggggtga
    15181 tcagttgcgg gttcaggcag ctttcataat aggcctgaat cttcatcatg cgagtgcgat
    15241 gacgctggta acgacgccag gcctgtacgc cattgtaaat ggcgctaccc agcatcgtca
    15301 gtagcagcaa tgtggtgctg agatagcgcc acaggccgga acgatccggg attggatgaa
    15361 ggccaatatg ctgcgtacca ttggcgtcag tgaagatttt tgtgacgata ccctcggcgt
    15421 taaacggcgt atgcatcagc atttgtgcca gtttctggaa agcgttccac tgttcttgcg
    15481 gcgggtagtc gtaaagtgat gccgaaggcc agggctgatc aacaaaatcg ctgccttcgt
    15541 cgctgacaat caggaatccg ccgggggccg gactgtttag tgattgtgcc gctcgcgccg
    15601 tttcatgcgt gataaacggc gcggtggagg ttgccaccag gttatccagc gattccgcac
    15661 tcaccgggcg taataacaca ttcacgccat cgagcttccc ggcgttggcg cgttttacca
    15721 gcgcgtccca gtctttactg ttgccgagat tgaccagcgc atttttcagt cgcacacagt
    15781 catctttggc agaacataaa tccgctgtct tcagtacaat gtcgccaaaa tcatcaagca
    15841 ataccatgcc ggatttttga attgctgaac gtaatgaggc actgacgcga gatccatctt
    15901 ccggtttagg gtgcagctgg cgattaactg cttcagtcaa tgccgtcgct ttgttgacca
    15961 gttcagattc tggtaatggc aatgagcggg cgtcgttcca gatgatctgc gagcagtcaa
    16021 acggtaaaaa aggtgaattg gttttcgcgc tccaggttcc ggaagtgcga atattacaca
    16081 ttcccgtacc gctgatacgc aatgtatcgc ctacccgcac gccagcgtca gccagttgtt
    16141 ttacgctggt ggcttcaatg gtctgtgcgc ctttcatcca tgagagagtg aatttcagcg
    16201 gcatatccag cgggatccag aataacagca taaacagcac cagcagcgag cccgcagcga
    16261 taatcgtact gcgcagccag tgctgtaacg gaaagttttt tacttcatca tgcagcgaaa
    16321 gatatcgccc ctgacgcact acgtggcggt cgagatagat atcaatatcg gtttgttgac
    16381 cgagatcctg agcaatgtat ggctgccagt gtgcgggata gaccaggtcg ataataccga
    16441 gtgaaatatt gttgatctgt tcctgatcgt tttcgccaaa caatccccaa cgacggggtg
    16501 taccgcgcag acaatgaatt tcccgcaggg aggattttgc cgggggcgcg aatagccccc
    16561 acagacctgc gcccagcagc agtaatgcgc cgcctgccag ccacggaaca aatacatccg
    16621 gggtaatcag gcagaaaaag aacatcagga aggaggcgac gatcagcaac gcttcacgca
    16681 gcccgcgcgg acgactcagc gcatattctt catgcgtttc tttgcgaata ttgagtagct
    16741 cgatttgctc actctcttcc ccgcgaatcg acgcctgcgt ggacggaaca ggttgcaagg
    16801 catagccgcg cgtttcctgc atgtactcct gcagcgtatg accgttgagt gaaataacca
    16861 gcggcagcga atcggtataa atcagttcaa cggtattctc atcgttgatg tattgttccc
    16921 agaaaggggg caggtggact tctaccgaat cgaggtagta acgccattta ttcggatcgt
    16981 cggtagagat gccgtaacgc gtgatagcgt gtgtcagcat catgacgttg ttgctttcgg
    17041 cattcagcgc cagagagatc ggtgccgcgc tagctcccgt tgggccaggc acctgtaata
    17101 cctgcgtcag gctctcaaga taattttcaa cggcgctacg ttcttcaggt gtgagtttac
    17161 gcgtttgcgc atccgcgaag gcgttggtcc agggcagctg acgccgtctg gatcgcactt
    17221 ttatcagcca tcccgcaagt agtgagcagg ccagcaaagc agctaaaaaa atcacaatgg
    17281 tgctcatgct ttccccatct tacttagtct gtcaggcgtg gtcagtcgca ccagacacaa
    17341 tacatgattc tgcggttggc tatcggcaag cgtggagttt aacttgagca tcattaagca
    17401 catcacagta accgctgatc atagcaaacc ctctgaaggc gggatatcag caaattcctg
    17461 gagttatttc aacctattga cttttaattt gatttgagga cgattattta ttaagttaca
    17521 taaatgtaaa taacatgcaa ttcacattgc cgaaagcgtg tccgatatcg cacaataacc
    17581 gctgatttca cagcatagat cccacgatga gcaaatcatt acaaaaaccc accattctga
    17641 atgttgaaac tgtagcccat tcccgactgt ttaccgtcga gagcgtggat ctggagttca
    17701 gcaatggcgt gcggcgtgtt tatgaacgaa tgcgtccaac caaccgggaa gcagtgatga
    17761 ttgtgccgat tgtggacgat cacctgatcc tgatccgcga atacgcagtg ggaactgaat
    17821 cctacgaatt aggtttttcg aaaggattaa ttgatccggg tgaaagcgtc tacgaagccg
    17881 ctaaccgcga gctaaaagaa gaggttggat ttggtgcgaa cgatctgact tttttgaaga
    17941 agctcagcat ggcaccgtct tacttttcca gcaaaatgaa tatcgtggtc gcgcaagatc
    18001 tctacccgga atcgctggaa ggcgatgagc cagagccgct accacaggtg cgctggccgc
    18061 tggcgcatat gatggatttg ctggaagacc ctgacttcaa cgaagcgcgt aatgtcagcg
    18121 cgctgttcct cgtgcgcgaa tggttgaaag ggcaggggcg agtgtaaatg cgtgaagttt
    18181 gcctgatgca caacgattat caggcttatg gggttcctgc aatatattga tttttcacga
    18241 ttttgttagc cggataacgc gttcacccgc atccggcata aacaaatgca ctttgtcagc
    18301 caactgaaaa ggcgccgaag cgccttttta atcagaacaa ttcctgtgcc tcaccattat
    18361 cgataatggt tgttcccacc tcgtgcaccg cctgttgtgt cggctgcgta ccttcgatga
    18421 aatactcttc gcggctgttg ccaccattag ctaactgccc ggtgctgcga tcgatattca
    18481 ccgtcacaat acccggaggc ggcgtcagcg gctgctccgg cacaccttca agaacggctt
    18541 tcatataagc gtcccatgca ggctgggcac tcttggcacc gccttcgtaa cctgagatct
    18601 gatctttaat cgctccggaa gccgttgtat gaccgagatt acgacggtga tcatcaaagc
    18661 caatccagac cgaggtcaca acgcccggac cgtaacccga gaaccacgca tctttcgaac
    18721 tgttagtggt accggttttc ccgccgatat cgcgacgctg caaatcacga cctgcacgcc
    18781 agccggtacc ctgccagcct ggttcaccaa agatattggt gttcaaagca ctcttaatca
    18841 ggaatgccag cggagtgttg atgacgtgcg gtgcgtactc ctgcgcgcca gtcttcgcca
    18901 ctaacgcctg atttgcctgc tccagctgcg gcattggtac agaaacattc tgctgctcgc
    18961 gggagatagc gacatcttca acatcgttat tttccagcac gttcgatttc tgcgtatcac
    19021 cgtaaatcac cggaatatcg cattccgggc aggctacttt cggtttcgct tcgaaaatca
    19081 cgccgccctg atcgttttca attttgctga taaaccacgg gtccaccagg aagccgccgt
    19141 tcgccatgac cgcgtagccg cgcgccacct gcattggggt gaaggacgct gaacccagcg
    19201 ccagcgattc ggtgtggaca atgttttgcg ccgggaagcc gaagcgttgc agatattctg
    19261 cagcgtagtc gacgcccatc gcccgcattg cacgtaccat caccacgttt ttcgactgac
    19321 ccagcccctg acgtaagcga attggaccag catactgcgg tggtgagttc ttcggctgcc
    19381 agtcagaacc gacacctgca tcccagcgag aaattggcac atcgttcaac atacttgcca
    19441 gcgtcagacc tttatccatc gccgcggtgt agaggaacgg tttgatgttg gaacccacct
    19501 gacgcagtgc ctgggtggcg cggttaaact tgctctgatt gaaatcaaag ccaccgacca
    19561 gcgccataac ggcaccattt tgcggattga tcgacaccag cgccgagttc acttccggca
    19621 cttgtgccag ccaccatgca tcgccaacct gacgaaccca gatttgctga cccgtttgca
    19681 gaacatcggt cactttacgc ggcgtcggtc cctgctgagt atccgaacgg taaggacgcg
    19741 cccagcgaac gccgtccata ctcaatgcga cagtcgaccc gtccgccagc attgccgtcg
    19801 cttcctgagg attggcgctg gtgactgcgg caggcagcag cggaccatag gtcggcagcg
    19861 ccttcagcgt atcggtaatc ttgttgttat cccacgccga ctcgcccact ttccacagca
    19921 catttgccgg gccgcgatag ccgtggcgca tgtcgtagtc cagcacgtta ttacgtaccg
    19981 cctgctgtgc ggcctgctgc actttgcggg tgatggtggt gtaaatgcga taaccgtctt
    20041 cataggcact ttcgccataa cggttataca tctcctggcg caccatttcg ctcaggtacg
    20101 gcgcagagaa agcaatctcc ggcgcgtgat agttagcgtt aatcgcctca gtgcgtgtct
    20161 gatcgaactg ttgttgggtg atataccctt catccagcat ccgcgacagt acgacgttac
    20221 gccgcgcgac ggcacgatcc attgagtaga gcggattaaa ggtggaaggc gctttcggca
    20281 gcccggctat cacagccatt tcgttcagcg tcagttggtc gaccgtttta ccgaaataga
    20341 cttgtgccgc agcaccgaca ccataggcgc ggtaaccgag gtaaatcttg ttcagataaa
    20401 gctcgaggat ctcgtctttc gtcagcaact gttcaatgcg aatcgcgagg aagacttcct
    20461 taatcttacg catcagcgtg cgttctggac tgaggaagaa gtttctcgcc agctgctggg
    20521 taatggtact cgccccttgt gacgcgtggc cggagaacag cgccacactt gccgcacgga
    20581 agatccccac cgggtcaacg ccgtgatgct cgtagaagcg gctgtcttcc gtcgcgataa
    20641 aggctttcac catctccggc gggatttgat ccaacgtaac cggaatacga cgtttctcac
    20701 cgtattgagc aatcagctcg ccatcggcgc tgtaaatctg catcggaatt tgcaggcgaa
    20761 catcttttaa tgtcgccaca tccggtagtt gtggctcgat gtagcggtat aggccataaa
    20821 tcgagcctgc tcccagcaga atgcaacaga ctgcaaggat caaaaaatac tttacgaact
    20881 tcactggaaa tttcccattt agtttcattt gggcagttta taaacaaacg tgcggtagta
    20941 taaaggcaag ccagacgcat tgatataccc gtcagagtga cgggtgataa ggagatcatc
    21001 acaatggcat ttaagatctg gcaaattggt ttgcatttac aacagcaaga agcagtagcg
    21061 gttgcgatcg tgcggggcgc aaaagaatgc tttttgcaac gctggtggcg gttgccgctg
    21121 gagaacgaca ttatcaaaga tgggcggatt gttgatgcgc agcggctggc taaaacgttg
    21181 ttaccgtgga gtcgcgaact gccgcagcgt catcacatta tgttggcgtt tcccgccagt
    21241 cgcacattac agcggtcatt tccgcgcccg tcgatgtccc tcggtgagcg ggagcaaacg
    21301 gcctggctgt cagggacgat ggcccgcgag ctggatatgg atccggactc cctgcgtttc
    21361 gattacagcg aagactcact cagtcccgcc tataacgtga ctgccgcgca aagcaaagag
    21421 ctggcaacgc tactcactct ggcagaaagg ttgcgtgttc atgtgagcgc gatcaccccg
    21481 gatgccagtg cattacagcg attcctgcct tttttacctt ctcatcagca atgtctggcc
    21541 tggcgtgata acgaacagtg gctgtgggcg acacgctgtc gctgggggcg caaactggcg
    21601 gtggggatga ctagcgcgaa ggagctggcg gcagcgttat ccgttgatcc cgaaagcgtc
    21661 gcgatatgtg gcgaaggcgg atttgatccc tgggaggccg tttctgttcg tcagccgccg
    21721 ctaccgccgc caggtggaga ctttgccatc gcgctggggc tggcgcttgg gaaggcgtac
    21781 tgatgaaccc gccaattaat tttttgccct ggcgacagca acgccggacc gcttttcttc
    21841 gtttctggtt gctgatgttc gttgcgcctc tgctgctggc cgtcgggata acgctaatac
    21901 tgcgtctgac agacaacgcc gaagcacgcg taaacaccgt tttgcttcag gcggaacaac
    21961 aactcgcccg cagcttacag ataacgaaac cacgtttgct ggagcgccaa caaattcgtg
    22021 agcagcgttt gcaacggcag cgccagcgac aatttacccg cgactggcaa tctgcgctgg
    22081 aagcactggc ggcgctttta cccgagcacg cctagctgac aacgataagc tggcagcagg
    22141 gaacgctgga gatcaagggg ctgacaacaa gcattaccgc gttaaacgca ctagaaacgt
    22201 cactccgcca ggatgcgtct tttcatctca atcagcgggg agccacgcag caggatgcgc
    22261 agggacgctg gcaatttgag tatcagttaa caaggaaggt tagcgatgaa catgttcttt
    22321 gactggtggt tcgccacatc accccgcctc cgccagtttt gctgggcatt ctggttgctg
    22381 atgttagtta cgctcatttt tctgtcatcg acacaccatg aagagcgcga cgcattaatt
    22441 cgactacggg caagtcatca ccagcagtgg gccacactgt atcgcctggt tgacaccact
    22501 cccttcagcg aagaaaaaac gctgcccttt tcgccactgg attttcagtt gcccggcgcg
    22561 caactggttt cctggcatcc atccgcgcag ggaggcgagt tggcgttgaa aacgctgtgg
    22621 gaagcagtgc cgtcggcatt tacacggctg gcagagcgca acgtcagcgt gagccgtttt
    22681 tcattaagcg tggaaggtga tgatcttttg ttcacgctac aactggagac gccgcatgag
    22741 ggttaaacgc tggttgttgg caggtattgt attgtgcctt ttaaccggta tgcgtgaccc
    22801 ttttaaaccg ccggaagatc tatgccggat tagcgaactt agccagtggc gctatcaggg
    22861 gatggtaggg cgaggcgagc gcatcatcgg tgtaataaaa gacgggcaaa agaaatggcg
    22921 acgggtgcag caaaacgatg tgctggaaaa cggctggaca attttacagc tgacgccaga
    22981 cgtactaacg ctgggtaccg ggacaaactg cgaaccgcca caatggttgt ggcaacggca
    23041 aggagataca aatgaagcaa tggatagccg cactactgtt gatgctgata cccggcgtac
    23101 aggcggcaaa gccgcaaaaa gtgacgctga tggtggatga cgttccggta gctcaggtgt
    23161 tgcaggcgct ggctgaacag gagaagttga acctggtcgt gtcgccagac gtcagcggta
    23221 cggtgtcgtt acatctaaca gatgttccct ggaagcaggc actacaaact gtagtgaaaa
    23281 gcgccggact gataacgcgg caggaaggca acattctctc agtgcattcc attgcctggc
    23341 agaataacaa tatcgcccgc caggaggcgg agcaggcgcg ggcgcaggca aatctgccgc
    23401 tggaaaatcg cagtataacc ctgcaatacg ccgacgcggg agaactggcg aaagcggggg
    23461 agaagctact gagtgccaaa gggagtatga ccgtcgataa acgcaccaat cgccttttgc
    23521 tacgagataa caaaacggcg ttaagcgcgc ttgaacagtg ggtagcgcaa atggatctgc
    23581 cggtcgggca ggttgagctg tcggcgcata ttgtcaccat taatgaaaaa agtttgcgtg
    23641 agttaggcgt gaaatggacg ctggccgatg cgcaacacgc tggtggcgtt gggcaagtca
    23701 ccacgcttgg tagcgacctc tccgtagcga cggcgacaac gcatgtcggt tttaacattg
    23761 ggcgcatcaa cggacgcttg ctggatcttg agctttccgc gctcgaacaa aaacagcagc
    23821 tggatattat cgccagtccg cgtctgctgg cctcacatct tcagcctgcc agcattaaac
    23881 aggggagcga aattccatat caggtttcca gcggggaaag tggcgcgacg tcggtggaat
    23941 ttaaagaggc cgtcctgggg atggaagtta cgcccacagt gttacaaaaa ggtcgcatcc
    24001 ggctgaaatt acacatcagc cagaacgttc cggggcaggt gctacagcag gccgatggcg
    24061 aagtgctggc gattgataag caggagatcg aaacgcaggt cgaggtcaaa agcggagaaa
    24121 cgttggcgct gggcggcatt tttacccgta aaaataaatc gggtcaggat agcgtaccgt
    24181 tgcttggcga cattccctgg ttcgggcaat tatttcgtca tgacggaaaa gaagatgaac
    24241 gacgcgagtt agtggtgttt atcacgccac gactggtttc cagtgagtaa acagccgtaa
    24301 aagcggtaat gtttttacgc tgaacgtgtt tcatctattt gacgcgcgca ggtatttagc
    24361 atacaaggag taccgatttg agagttggtg ctcttcgctg cctgcgttcc atgatgatga
    24421 tttatcattc aggcggcatt ttgctgtctt ttttacgcta atcttacccg gtgatttatc
    24481 gccagagcgg tggtagcaag gcagcgcgct tgcagcgacc agatatgcag agggatgggt
    24541 gatttattca gttgccaaac ccgctggagt attgagataa ttttcagtct gactctcgca
    24601 atatcttatg aggtttcagt tcatgtcctg cggcgctctc tgagcgaagc gggtttatca
    24661 ttaacgaata gtcttagtag taccgaaaaa atggcagaga aacgcaatat ctttctggtt
    24721 gggcctatgg gtgccggaaa aagcactatt gggcgccagt tagctcaaca actcaatatg
    24781 gaattttacg attccgatca agagattgag aaacgaaccg gagctgatgt gggctgggtt
    24841 ttcgatttag aaggcgaaga aggcttccgc gatcgcgaag aaaaggtcat caatgagttg
    24901 accgagaaac agggtattgt gctggctact ggcggtggct ctgtgaaatc ccgtgaaacg
    24961 cgtaaccgtc tttccgctcg tggcgttgtc gtttatcttg aaacgaccat cgaaaagcaa
    25021 cttgcacgca cgcagcgtga taaaaaacgc ccgttgctgc acgttgaaac accgccacgt
    25081 gaagttctgg aagcgttggc caatgaacgc aatccgctgt atgaagagat tgccgacgtg
    25141 accattcgta ctgatgatca aagcgctaaa gtggttgcaa accagattat tcacatgctg
    25201 gaaagcaact aattctggct ttatatacac tcgtctgcgg gtacagtaat taaggtggat
    25261 gtcgcgttat ggagaggatt gttgttactc tcggggaacg tagttaccca attaccatcg
    25321 catctggttt gtttaatgaa ccagcttcat tcttaccgct gaaatcgggc gagcaggtca
    25381 tgttggtcac caacgaaacc ctggctcctc tgtatctcga taaggtccgc ggcgtacttg
    25441 aacaggcggg tgttaatgtc gatagcgtta tcctccctga cggcgagcag tataaaagcc
    25501 tggctgtact cgataccgtc tttacggcgt tgttacaaaa gccgcatggt cgcgatacta
    25561 cgctggtggc gcttggcggc ggcgtagtgg gcgatctgac cggcttcgcg gcggcgagtt
    25621 atcagcgcgg tgttcgtttc attcaagtcc cgacgacgtt actgtcgcag gtcgattcct
    25681 ccgttggcgg caaaactgcg gtcaaccatc ccctcggtaa aaacatgatt ggcgcgttct
    25741 accagcctgc ttcagtggtg gtggatctcg actgtctgaa aacgcttccc ccgcgtgagt
    25801 tagcgtcggg gctggcagaa gtcatcaaat acggcattat tcttgacggt gcgtttttta
    25861 actggctgga agagaatctg gatgcgttgt tgcgtctgga cggtccggca atggcgtact
    25921 gtattcgccg ttgttgtgaa ctgaaggcag aagttgtcgc cgccgacgag cgcgaaaccg
    25981 ggttacgtgc tttactgaat ctgggacaca cctttggtca tgccattgaa gctgaaatgg
    26041 ggtatggcaa ttggttacat ggtgaagcgg tcgctgcggg tatggtgatg gcggcgcgga
    26101 cgtcggaacg tctcgggcag tttagttctg ccgaaacgca gcgtattata accctgctca
    26161 agcgggctgg gttaccggtc aatgggccgc gcgaaatgtc cgcgcaggcg tatttaccgc
    26221 atatgctgcg tgacaagaaa gtccttgcgg gagagatacg cttaattctt ccgttggcaa
    26281 ttggtaagag tgaagttcgc agcggcgttt cgcacgagct tgttcttaac gccattgccg
    26341 attgtcaatc agcgtaacaa caagaaaggt caggccgctt atcaagcggt ctattagctt
    26401 caggttaatt gcaacgtggt aagcattaac cttttagtgg ggtgttaaat ggatgaattc
    26461 aaaccagaag acgagctgaa acccgatccc agcgatcgtc gtactggtcg ttctcgtcaa
    26521 tcttctgaac gttctgagcg tactgaacgt ggcgaaccgc agatcaattt tgatgatatt
    26581 gaacttgatg acactgacga tcgccgtccg actcgtgcgc aaaaagagcg caatgaggaa
    26641 ccggaaatcg aagaagaaat tgacgaatcc gaagatgaaa ccgtggatga agagcgcgta
    26701 gagcgtcgtc cgcgtaagcg caaaaaagca gccagtaaac ccgcttctcg tcagtatatg
    26761 atgatgggcg tcggcattct ggttctactg ctgttgatca tcggtatcgg ttctgcgcta
    26821 aaagccccct cgacctcttc cagcgatcaa accgcgtctg gcgagaagag tattgatctt
    26881 gcaggcaatg cgaccgatca ggcgaatggg gtgcagccag cgccgggaac cacgtctgcg
    26941 gaaaatactc agcaggatgt ttctctgcca ccgatctctt ctacgccgac tcaagggcaa
    27001 accccggcgg caacggatgg tcaacaacgt gttgaagtgc agggtgacct gaacaatgcg
    27061 ctgacccagc cacaaaatca gcaacagttg aacaatgtgg cggtcaattc cacattgccg
    27121 accgaacccg cgactgtcgc gcctgttcgc aatggcaatg catcgcgtga cacggcgaaa
    27181 acgcaaaccg ctgaacgtcc gtccactacg cgcccagctc gtcagcaggc ggtgattgaa
    27241 ccgaaaaaac cgcaagcaac cgtgaaaacg gagccgaagc cggtagcaca gacgccgaag
    27301 cgtactgaac cagctgcccc tgtggcgagc acgaaggcac cgactgcgac ttctacgcca
    27361 gcaccaaaag agacggcgac tacggctcca gtacagacgg catccccggc gcaaaccacg
    27421 gcaacaccag ccgctggagg gaagaccgca ggtaatgttg gttcgttgaa atcggcaccg
    27481 tccagccatt acactctgca gctgagcagt tcctctaact acgacaacct gaacggttgg
    27541 gcgaagaaag agaatctgaa aaactacgtt gtctatgaaa cggcgcgtaa tggtcagccg
    27601 tggtatgtcc tggtttctgg cgtgtacgct tcgaaagaag aggcgaaaaa agcggtatct
    27661 acattgccag cagatgttca ggccaaaaac ccgtgggcga aaccgctgcg tcaggtacag
    27721 gccgatctga agtaatcaag gttatctccc gcaatggttt atcgttgcgg gagctgcctg
    27781 aagcgctgga tgctgtcgga gctttctcca cagccggaga aggtgtaatt agttagtcag
    27841 catgaagaaa aatcgcgctt ttttgaagtg ggcagggggc aagtatcccc tgcttgatga
    27901 tattaaacgg catttgccca agggcgaatg tctggttgag ccttttgtag gtgccgggtc
    27961 ggtgtttctc aacaccgact tttctcgtta tatccttgcc gatatcaata gcgacctgat
    28021 cagtctctat aacattgtga agatgcgtac tgatgagtac gtacaggccg cacgcgagct
    28081 gtttgttccc gaaacaaatt gcgccgaggt ttactatcag ttccgcgaag agttcaacaa
    28141 aagccaggat ccgttccgtc gggcggtact gtttttatat ttgaaccgct acggttacaa
    28201 cggcctgtgt cgttacaatc tgcgcggtga gtttaacgtg ccgtttggcc gctacaaaaa
    28261 aacctatttc ccggaagcag agttgtatca cttcgctgaa aaagcgcaga atgccttttt
    28321 ctattgtgag tcttacgccg atagcatggc gcgcgcagat gatgcatccg tcgtctattg
    28381 cgatccgcct tatgcaccgc tgtctgcgac cgccaacttt acggcgtatc acacaaacag
    28441 ttttacgctt gaacaacaag cgcatctggc ggagatcgcc gaaggtctgg ttgagcgcca
    28501 tattccagtg ctgatctcca atcacgatac gatgttaacg cgtgagtggt atcagcgcgc
    28561 aaaattgcat gtcgtcaaag ttcgacgcag tataagcagc aacggcggca cacgtaaaaa
    28621 ggtggacgaa ctgctggctt tgtacaaacc aggagtcgtt tcacccgcga aaaaataatt
    28681 ctcaaggaga agcggatgaa acagtatttg attgccccct caattctgtc ggctgatttt
    28741 gcccgcctgg gtgaagatac cgcaaaagcc ctggcagctg gcgctgatgt cgtgcatttt
    28801 gacgtcatgg ataaccacta tgttcccaat ctgacgattg ggccaatggt gctgaaatcc
    28861 ttgcgtaact atggcattac cgcccctatc gacgtacatc tgatggtgaa acccgtcgat
    28921 cgcattgtgc ctgatttcgc tgccgctggt gccagcatca ttacctttca tccagaagcc
    28981 tccgagcatg ttgaccgcac gctgcaactg attaaagaaa atggctgtaa agcgggtctg
    29041 gtatttaacc cggcgacacc tctgagctat ctggattacg tgatggataa gctggatgtg
    29101 atcctgctga tgtccgtcaa tcctggtttc ggcggtcagt ctttcattcc tcaaacactg
    29161 gataaactgc gcgaagtacg tcgccgtatc gacgagtctg gctttgacat tcgtctggaa
    29221 gtggacggtg gcgtgaaggt gaacaacatt ggcgaaatcg ctgcggcggg cgcggatatg
    29281 ttcgtcgccg gttcggcaat cttcgaccag ccagactaca aaaaagtcat tgatgaaatg
    29341 cgcagtgaac tggcaaaggt aagtcatgaa taagtttgaa gatattcgcg gcgtcgcttt
    29401 tgatcttgat ggtacgctgg tcgacagtgc tcccggtctt gctgctgcgg tagatatggc
    29461 gctgtatgcg ctggagttgc ccatcgcagg tgaagaacgc gttattacct ggattggtaa
    29521 cggcgcagat gttctgatgg agcgcgcatt gacctgggcg cgtcaggaac gtgcgactca
    29581 gcgtaaaaca atgggtaaac cgcccgttga tgacgacatt ccggcagaag aacaggtacg
    29641 tattctgcgt aaactgttcg atcgctacta tggcgaggtt gccgaagagg ggacgttttt
    29701 gttcccgcac gttgccgata cgttgggcgc gttgcaggct aaaggcctgc cgctaggcct
    29761 ggtcaccaac aaaccgacgc cgttcgtcgc accgctgctc gaagccttag atattgccaa
    29821 atactttagc gtggttatcg gcggcgatga tgtgcaaaac aaaaaaccgc atccggaccc
    29881 gctgttactg gtggctgagc ggatgggaat tgccccacaa cagatgctgt ttgtcggcga
    29941 ctcacgcaat gatattcagg cggcaaaagc ggcaggttgc ccatcagttg gcttaaccta
    30001 cggatataac tacggcgagg ctatcgatct cagccagcct gatgtaattt atcagtctat
    30061 aaatgacctt ctgcccgcat tagggcttcc gcatagcgaa aatcaggaat cgaaaaatga
    30121 ctaagcccat cgtttttagt ggcgcacagc cctcaggtga attgaccatt ggtaactaca
    30181 tgggtgcgct gcgtcagtgg gtaaacatgc aggatgacta ccattgcatt tactgtatcg
    30241 ttgaccaaca cgcgatcacc gtgcgccagg atgcacagaa gctgcgtaaa gcgacgctgg
    30301 atacgctggc cttgtatctg gcttgtggta tcgatcctga gaaaagcacc atttttgttc
    30361 agtcccacgt accagaacat gcgcagttag gctgggcact gaactgttat acctacttcg
    30421 gcgaactgag tcgcatgacg cagtttaaag ataaatctgc gcgttatgcc gagaacatca
    30481 acgctggtct gtttgactat ccggtgctga tggctgcgga catcctgctg tatcaaacta
    30541 atctggtacc ggtgggtgaa gaccagaaac agcacctgga actgagtcgc gatatcgccc
    30601 agcgtttcaa cgcgctgtat ggcgagatct ttaaggtgcc ggagccgttt attccgaaat
    30661 ctggcgcgcg cgtaatgtcg ctgctggagc cgaccaagaa gatgtccaag tctgacgata
    30721 accgcaataa cgttatcggc ctgctggaag atccgaaatc ggtagtgaag aaaatcaaac
    30781 gcgcggtcac tgactccgac gagccgccgg tagttcgcta cgatgtgcag aacaaagcgg
    30841 gcgtttccaa cctgctggat atcctttctg cggtaacggg ccagagcatc ccagaactgg
    30901 aaaaacagtt cgaaggcaag atgtatggtc atctgaaagg tgaagtggct gatgccgttt
    30961 ccggtatgct gactgaattg caggaacgct atcaccgttt ccgcaacgat gaagccttcc
    31021 tgcaacaggt gatgaaagac ggcgcagaaa aagccagtgt acacgcttcc cgtacgctga
    31081 aagcggtgta cgaagcgatt ggttttgtgg cgaagccgta atttaacatt aatgccaaaa
    31141 accggggaaa cccggttttt ttatcctcat ttgcaataat ccgaaaaaat gtgaagcgcc
    31201 tcgccgtttc cacatctcgt cgttgccact tatcttcatt ttaatgaaga tataaatatt
    31261 cattttattg aaaatatatt atgcgtcgaa cgtttatcaa aaaagaaggc gtcgtcatca
    31321 cgacgctggc ccgttatttg ttgggtgaaa aatgcggtaa tcgactgaaa accatagatg
    31381 agctggcaac tgaatgccgt tcatcggttg gcctgacgca ggccgcgttg aaaacgttgg
    31441 aatcaagcgg agcgatacgg attgaacgcc gtgggcgcaa tggcagttat ctggtcgaga
    31501 tggataacaa agcattgctg agtcatgtgg atatcaacaa cgtggtatgt gcgatgcctt
    31561 tgccctatac ccgcttgtac gaaggtttag cgagcgggct gaaagctcag tttgatggca
    31621 ttccttttta ctatgcgcat atgcgcggcg cggatattcg cgtggagtgt ttacttaacg
    31681 gcgtgtatga catggcggtg gtttcccgac tggcggcgga aagttatctc acgcaaaaag
    31741 ggttatgcct cgcgctggag ttggggccgc acacctacgt tggcgagcac cagttgattt
    31801 gccgtaaagg cgagtccgca aacgtgaagc gcgtggggct ggataaccgt tcggcggatc
    31861 agaaaatcat gaccgatgtc ttttttggcg atagtgatgt ggaacgtgtc gatctctctt
    31921 atcacgagag tttacaacgc attgttaaag gcgatgtcga tgcggttatc tggaacgtgg
    31981 tggcggaaaa cgaactgacc atgctgggat tagaggcgac gccgctcaca gacgatccgc
    32041 gatttttaca ggccaccgaa gccgtggtcc tgacgcgagt cgatgattac ccaatgcaac
    32101 aactgctgcg tgccgttgta gataaacacg ccctgctcgc ccatcaacaa cgggtagtga
    32161 gtggggaaca ggaaccgagt tattaaacga aaggcatctg atatggaaac cagactcaac
    32221 ctgctttgcg aggcaggcgt tattgataag gacatctgca aaggcatgat gcaggtcgtc
    32281 aacgtactgg aaacagagtg ccatctgccg gtgcgcagtg agcaaggaac gatggcgatg
    32341 acacatatgg cgagtgcact gatgcgcagt cgccgtggtg aagaaataga gccgctggat
    32401 aacgaattgc tggcagagct ggcgcaatcc agccactggc aagccgttgt gcaattgcat
    32461 caggtgttgt tgaaggaatt cgcactggaa gttaacccgt gtgaagaagg ctatttgctg
    32521 gccaaccttt atggattatg gatggctgct aacgaagagg tttgaagctc ccgacaaatc
    32581 cacgtcgcac aacctaaata ttcaaaaaaa tgaatattta attcaaggaa cagaaacgag
    32641 gcaacaagat gtttgtagaa gcattgaaac gccagaaccc ggcgctgatt tccgccgcac
    32701 taagtttgtg gcagcagggc aagatcgccc cggacagctg ggtgatcgac gtggatcagg
    32761 tactggaaaa cggtaagcga ctgattgaga cggcgcggct ttacggcatt gaactgtatc
    32821 tgatgaccaa gcagtttggc cgtaacccat ggctggcgga aaaattgctg gcattaggtt
    32881 acagcggcat tgtggcggtg gattacaaag aggcgcgagt catgcgccgc gctggtttgt
    32941 cggtggcaca tcaggggcat ctggtacaaa tcccttgtca tcaggttgct gacgccgttg
    33001 aacagggcac cgacgtcatc accgtgttta ctctcgacaa agcgcgggaa gtttctgcgg
    33061 cagcggtgaa ggccggacgt gtgcagtttg tgctgctcaa agtttatagc gacgatgatt
    33121 ttctttatcc ggggcaggag agcggttttg tacagcattc gctgcacgag gtggttgctg
    33181 aaattaagaa actgccgggg ctgcatttag ccggacttac ccatttccct tgcctgcttt
    33241 gggatgaggc tgccggaaaa gttttgccga caccgaatct tcacacgctg atacaggcac
    33301 gggatcaact ggcgaaatct ggtattgcac ttgagcaact gaacgcgcct tcagcgacca
    33361 gctgcacttc gctgccatta ctggcgtaat acggtgtgac tcatgccgaa cccggtcatg
    33421 cgctgacggg cactattccg gcaaaccagc agggcgatca gtctgaacgt atcgcgatgc
    33481 tctggttgag tgaaatctcc catcatttcc gtggcgacag ctactgctac ggcggcggtt
    33541 actaccgtcg tggtcatgcg caacatgcgt tggtgtttac gccagaaaat caaaagatta
    33601 ctgaaaccaa tctcaaaact gtggatgaca gcagtatcga ctacaccctg tcgctggcag
    33661 gcgagtttcc ggtaagcagt gcagtggtgc tctgtttccg cacgcagatt tttgtcaccc
    33721 gtagcgatgt ggtgctggtg tccggtattc accgtggcga accgaaaatc gttggtcgtt
    33781 atgacagtct tggaaactct ctgggggcgt aatggcgcga tttgtggtgt tagtgattga
    33841 tagctttggc gtaggggcaa tgaaagatgt cacgctggtg cgtccgcaag atgcgggagc
    33901 gaatacatgt ggtcacatcc tgagccagtt gccgcatttg cagctaccaa cgctggagaa
    33961 gctggggcta atcaacgcat tgggttatgc gccaggcgat atgcagccgt cagattccgc
    34021 aacctggggc gtggcagagc tgcaacatga aggtggcgat acctttatgg ggcatcagga
    34081 aattttaggc acgcgcccgt taccgccgct gcggatgcct tttcgcgatg tgattgaccg
    34141 tgttgagcag gcattagtat ccgccggttg gcaggtggag cgccgtggcg atgaactgta
    34201 atttctgtgg gtcaatcagg cggttgcgat tggcgataat ctcgaggcgg atttaggcca
    34261 agtctataac attaccgcca atctctctgt gatctctttt gacgacgcaa tcaaaattgg
    34321 tcgtatcgtg cgtgagcagg tacaggtcgg tcgggtcatt acatttggtg gcctgttaac
    34381 cgacagtcaa cgcattctcg atgccgcaga aagcaaagaa gggcgcttta ttggtatcaa
    34441 tgcgccgcgt tctggcgctt atgacaacgg cttccaggtc gtgcatatgg gctacggtgt
    34501 cgataaaaaa gtgcaggtgc cgcaaaaact gtatgaggcg ggcgtgccaa ccgtgctggt
    34561 gggtaaggtg gcagatatcg tcagcaatcc ttatggcgtg agctggcaaa acctggtgga
    34621 tagccagcgg attatggata tcaccctcga cgaatttaac acccatccga cggcgtttat
    34681 ttgcaccaat atccaggaaa ccgacctcgc tggtcatgca gaagacgtcg cacgttatgc
    34741 cgaacgtttg caggtcgttg accgtaacct tgcccggctt gttgatgcga tgcagccaga
    34801 tgattgcctg gtcgtgatgg cggatcacgg caacgatccg accattggtc acagccacca
    34861 tacccgcgaa gtggtgccgc tgctggttta tcagcaaggc ctggctgcta cgcagctcgg
    34921 tgtgcgcacc acgctttctg atgtgggagc taccgtgtgt gaatttttcc gtgcgccacc
    34981 gccacaaaat ggtcgctctt ttctttcctc cctccggttt gcaggagaca ccctatgagt
    35041 tttgatccga cgggttacac cctggcccat gagcatctgc atattgatct ctccggcttt
    35101 aaaaacaacg tggactgccg ccttgatcag tatgcgttca tttgccagga gatgaacgac
    35161 ctgatgaccc ggggtgtacg taatgtgatt gagatgacca accgttacat ggggcgcaat
    35221 gcgcaattta tgctcggtgt aatgcgcgaa accgggatca acgtggtggc ctgtactggt
    35281 tattaccagg atgcgttttt cccggaacat gtggtgaccc gcagcgtgca ggaactggcg
    35341 caggagatgg tcgatgaaat tgaacagggt atcgatggca cggagctgaa agccgggatc
    35401 atcgcggaga tcggctccag cgaaggaaag attacgccgc tggaagagaa ggtatttatt
    35461 gccgctgcgc tggcgcataa ccagaccgga cgcccgatct ccacgcatac gtcgttcagc
    35521 accatggggc tggaacaact ggctctgttg caagcccacg gagttgatct ttcgcgcgtc
    35581 accgttggtc actgcgatct gaaagacaac ctcgacaaca ttttgaagat gatcgatctc
    35641 ggcgcgtacg tgcagttcga caccattggt aagaacagtt actacccgga cgaaaagcgc
    35701 attgcgatgc tccacgcgct acgtgaccgt gggttgctga accgcgtcat gctgtcgatg
    35761 gatattacgc gccgctccca tttaaaagcc aatggtggtt atggctatga ctatttattg
    35821 accaccttta ttccgcaatt gcgccagtca ggattcagtc aggccgatgt ggatgtgatg
    35881 ttacgtgaaa ctccctctca atttttccaa taaggacaga ctcatgaaaa agattggcgt
    35941 tgcaggctta cagcgtgagc agattaaaaa aaccattgaa gcgacggcta ctggcagttt
    36001 tgaagttttc attcacaacg acatggaagc ggcaatgaaa gtgaaatccg ggcaactgga
    36061 ttactacatc ggtgcgtgta ataccggtgc gggcgcggca ttgtcgattg ccatcgcggt
    36121 gattggctat aacaaaagtt gcaccattgc caaaccaggc attaaagcga aagacgagca
    36181 tatcgccaaa atgatcgctg aaggaaaagt ggcgtttggc ctttccgttg agcacgtcga
    36241 acacgcgatt ccgatgctga ttaaccatct gaaataaaag gcacgactat ggatctgtat
    36301 attcagatta tcgtggtggc gtgcctgacg ggtatgacat cgcttctggc gcatcgctcg
    36361 gcggctgttt ttcatgacgg tatccgcccg atcctgccgc aactgattga aggctatatg
    36421 aaccgtcgcg aggcggggag tatcgctttt ggtctgagca ttggttttgt ggcctcggtg
    36481 gggatctctt ttaccctgaa aaccgggctg ctcaacgcat ggttactctt tcttcctacc
    36541 gatatcctcg gcgtactggc gataaacagc ctgatggcgt ttggtcttgg cgctatctgg
    36601 ggcgtgttga tccttacttg cctgttgcca gtaaaccagc tgctgaccgc gctgccggtg
    36661 gatgtattag gtagcctcgg ggaattaagc tcgccggtgg tttctgcttt tgcactgttc
    36721 ccgctggtgg cgattttcta ccagtttggc tggaagcaaa gtctggtcgc cgccgtggtt
    36781 gttctgatga cccgtgtggt agtcgtgcgc tatttcccac atcttaaccc tgaatccatc
    36841 gaaatcttta ttggcatggt gatgctgctg gggatcgcga taactcacga cctgcgtcat
    36901 cgtgatgaaa atgatattga tgccagcggg ctttcggtgt ttgaagaacg cacgtcgcgg
    36961 attatcaaaa acttacccta tatcgccatc gtgggagcat tgattgccgc cgttgccagc
    37021 atgaagattt tcgccggcag tgaagtgtcg atcttcactc tggagaaagc gtactccgca
    37081 ggcgtaacgc cggaacaatc gcaaacgctg atcaaccaag cggcactggc agaatttatg
    37141 cgcgggctgg gttttgtacc gttgattgcc accaccgcct tagcaacggg tgtgtatgca
    37201 gttgcgggct ttacctttgt ttatgcggtg ggctatctct cgccgaatcc gatagttgca
    37261 gcggtattag gcgcagtggt tatttcggcg gaagtcttgc tgcttcgttc gatcggcaaa
    37321 tggctgggac gctacccgtc ggtgcgtaat gcgtcggata acatccgtaa cgccatgaat
    37381 atgctgatgg aagtggcgct gctggtcggt tcgattttcg cagcaattaa gatggcgggt
    37441 tataccggat tctctatcgc ggttgccatt tacttcctca acgaatccct gggccgtccg
    37501 gtacagaaaa tggcggcacc ggtcgtggca gtaatgatca ccggtattct gctgaatgtt
    37561 ctttactggc ttggcctgtt cgttccggct taaggaaggc acctatgaag acgtttcctc
    37621 tgcaaagtct gacgattatt gaggcgcagc aaaagcagtt tgcgctggtg gatagcattt
    37681 gtcgccattt ccccggcagc gagtttctta ctggcggtga tttaggctta acgccaggac
    37741 tgaatcaacc gcgcgttacc cagcgtgtgg aacaggtgct ggctgatgca tttcacgcac
    37801 aggctgcggc gctggtgcag ggtgcgggta ctggcgcgat tcgtgcggcg ttggcggctt
    37861 tgctcaaacc ggggcagcgt cttctggtac atgatgcgcc tgtttacccg acgacacagg
    37921 tcattattga gcagatgggg ctgacgctta ttactgctga tttcaatgac ctgtcggcac
    37981 tgaagcaggt cgtcgacgag caactaccgg atgcggcgct ggtgcagcat acgcgccagc
    38041 agccgcagga cggctacatt ctggcagatg tgctggcaac attgcgctcg gctggcgttc
    38101 ctgcattaac cgatgacaac tatgcggtga tgaaagtggc gcgaattggc tgcgaatgcg
    38161 gggcaaatgt ctcgactttt tcctgcttca agctgtttgg gccggagggt gttggcgcgg
    38221 tggtaggcga tgccgatgtt attagtcgga ttcgctccac gctctattcc ggcggcagcc
    38281 agattcaggg cgctcaggcg ctggaagtct tgtgtgggct ggtgtttgcg ccagtgatgc
    38341 acgcggtgca ggcgggggta tctgaacggt tgctggcttt gcttaacggt ggtgcggtgc
    38401 cggaagtgaa aagcgcggtg attgctaatg cgcagtcgaa ggtgttgatt gtcgagtttc
    38461 atcagccgat tgccgccaga gtgctggaag aggcgcaaaa gcgcggtgcc ttgccttacc
    38521 cggtgggtgc agagtcgaaa tatgaaatcc cgccgctctt ttatcgcctt tccggaacgt
    38581 ttcgccaggc gaatccacaa tcagaacatt gtgcgattcg cattaacccg aatcgcaacg
    38641 gtgaagagac ggtgctgcgg attttgcgtg agagtattgc cagtatttaa tgctggatag
    38701 caccgtcaag ctaaattccg tactgaacgg tcccctcgcc cctttgggga gagggttagg
    38761 gtgaggggaa cgtacgggat gtaaaattca acgcgccagc atcgccggat gcgatgctgg
    38821 cgcatcttat ccggcctacg gttaatgtct tttattatca atcgttaagg tgatgcgatt
    38881 ggcgcggcag aagagttcgg agacgtgcac cggtttgtca ttctgatcaa aggcgatttt
    38941 gctgatgcga aacaacggtt cgcccagttc gcatttcagc cattttgcct gcgggcgggt
    39001 ggcggcaaag atatcgatgg tctttttgtc gctgaccact cgcgtgtcaa aacgctcctg
    39061 aaataactga taggtggagc ttccttcgac gtaaatctca tcaaagtcag gataacgcga
    39121 cagcgggatc catgaactat caataaacag cggctcttta tcgagataca tcacccggca
    39181 gagatggaac acttcgctgt ttccggggat gttcagcttt tcgcaaaacg gcgcggcgct
    39241 gacccgttcc tgttcgatca ctttctcttt cgtcgccttg ccttgtgaga cgccaaaatc
    39301 ggtaaaacca ctgacagtaa gcagggcgtt ttcaactttc tggctttgta caaaggtgcc
    39361 tttgccctgc cagcggatca gtacgccgtc tgccactaag tcgctgatag ctttgcgaat
    39421 agtaatgcgg ctgacgttat attgcgtaca aagctcgttt tcggtaggga tctgttgccc
    39481 ggcctggtaa accccctgcg cgatatcatc cagcagccgc tggcggacgg tagcgtagag
    39541 gagttgatga gagtagcggt ccgtagctga cataatcgac cctgaaataa agaatgaatt
    39601 acacggatta tagggtcgtt ttccggcaag taacagcacg ttggcgcgga gatgctcacg
    39661 ccaacgttat acctaccagg caccgtgata ctgaatggtt ttcgccgcgc acgccgttcc
    39721 ctgcgccatc gcctgcggta atgtcatccc cgcagaccag ccgcaaagga atccggcaat
    39781 gaacgaatct ccggcaccca tggtgtcgat aaccgtcacc ggttcaggag cctgacgcca
    39841 gaactgcgcg ccatcccagg caatgctgcc gttttcaccc agcgtgacaa tcactgttcc
    39901 tgcgccacgg gcaacaatcg ctttcatctt cagacgcagc gcttcgtctt cttgcggtgc
    39961 ggaggcaaag gcaaaatcga gatgcggcac cagtgtctgc cagagcgggc tgtcccactt
    40021 gtcggagaag tcgaaagcgg taagtttgcc cgcagcgtgc agctgtggga atgcgtcttc
    40081 cgcatgtccc cagattgccg cgtgcacaat gtcatactgc gccagccagg cgtaatcctc
    40141 ttcactcagg gcaaagtcgg ccatcacgcc ttcggtgtag tcgccaaaaa cgcgatcatt
    40201 gtcgtgcagt tccacctgag tttgtgcggt aacgccgtgt ttcgtatgga catggctgat
    40261 atcgacgccc atgcgggcga gatcctgctt cagctttgtg ccgtagtcat cgtcacccac
    40321 caaggtaatg catcccggct gtatgccgta gcgagtgcag tacaccgcca cattgaccgc
    40381 attaccgcca gaaaacgctt tattcagttg cgggtagata tcgacgcagt tatcgccgat
    40441 tgtcgccagg gttttcatct ctcatcctcc ggcagcagcg cgcgaaagcg ttccagcgcc
    40501 tggcgggcat agagtctggg ctcgttcata tacatcgtca ccagctccac cgtacagtaa
    40561 ccctcatagc cccgctcaat aatatcgcgc atcagttccc gcagcggcat tttgccttct
    40621 ccaggaatgt aatgcgtgtc gctggccccg tcgctgtcga caatatgcag atgacgataa
    40681 tttatcgccc agtttgtcga aataactcat caccggttcc gcctggacat acggcgcgca
    40741 aatgtcgacc atgctgaaca ggcgcggcga aggcaccagc gccagcgcat gaagcacatc
    40801 attagcatta cacacgacgt tcgattcata cggcgttaac ggttcgagga ttagatccat
    40861 gccgatgttt tccgcgtact cacacagttc gcttaggttc tctgccagcc gtccccagat
    40921 aacattaggt ggcgtgagat agcctgcgtg ggccgcggaa atcagcgtat aacccgcgtt
    40981 catctctttt gccatatcca tcgccagctt gatcatgtcg aggctttcgc gacgcatatg
    41041 ttcatcgccc agcatcatgt tatacggata gccgttggtt tctggcgtat agccgataat
    41101 cggcatctga tacgtctgcg ccagcgcctt gatttgtttg atgccgcccg cttttaagtc
    41161 cggcgcgaac gcgtgcgggc gaccgcccca aatttcgatg ccgtcgtaac ccagctcgct
    41221 tgcatcacga aatgcgtgtt caataggcag ccgctggtgg ccgcaggtaa acatacctgt
    41281 tttcatcggg taactccttg cgtaaatccg gcccgttgcc gggccgggag agggattaat
    41341 attccaccag accaccgtaa tagcggcgtt catccgggtt gtgatctttg taaatagaca
    41401 ggtagtagca gagccactcc attggcacga acatcaggaa cggtgccagc cacgggtgca
    41461 gcccttgcga aatttcggcg taatcgatga cgatcacgtt gtcagtacgc tgtttaacaa
    41521 agttaatggc gcattcggtg gtgtggcgac tttcatcatt gccgagcagg aacaggaacg
    41581 gaacgcccgg ttcgacaatc tccagcgggc catggcggaa ctctccgctc tcaatcacgc
    41641 agccgtgcgt ccaggtaaat tccatcagcg ttacaatgcc ttctttgtaa cccagcggac
    41701 gcagcggacc cgcagcaacg gtataaatca tcggccactg gctggccagt tcaccaagct
    41761 ggcggccttt ttcttcccag gtgcgtacca gatgaccgag cgcattcggc aacggcttga
    41821 gatcgttttt gatcttgccg atttccgcgt tcggcgcgag gcgggtgatc atctccagca
    41881 ccacgctgta gcagagcagc aggtgaattt cccagataca gtcggcctga taatcaatgc
    41941 taaattccgc cgccgaggta atcgggctgt ccgcgcgttt ggtgaacgcc gcagtgagtg
    42001 cgccgcaggc ccggcccagc tccagcgctt tgattacctc ttccgtttta ccgtagtcag
    42061 aaacgccaat caccgcgcaa cgatcgtcga ggcgatacgg ggtgttatcg cagaactccc
    42121 agccggaaat ggcgtagacc tgaagatcgg aaaagcgatc cgccagatgt ttcgccgttt
    42181 gcgccgcgtt gagtggcgag ccgcaggcaa cgaaataaat acgatcaatg tcgcgcttca
    42241 ccatctcttc cacgatggcg tgcaccagcg gaacgtcatg gctgagaact ttttccactt
    42301 cctgaaccat attttcggtg accagaaagt ccacggtgct tttatcaata tccaacattt
    42361 tattttctcc agataacatt acgacaaagc gttgagctgg cggctacgct tcgcccagaa
    42421 ggcgtaagca ggcagaccag tagcaataac gatgacggcg cagataaggc cgggaatcgg
    42481 tgcccagaca aaggttgagg cgaccagaat gaggcttgac gcaatggcga gggtggtcat
    42541 cagcccgaaa gccggagtac gccacagcgg tttgtaatcg tcgcgtttac gacaccagat
    42601 gatggagccg aaggtgaggg tatttttgaa acacatcacc agggtgaaat aacccagcag
    42661 gctggtgaga tcggaaacga agatgaagaa gatccccagc gccccttgca ggatgatgga
    42721 gacatccggc gtgttgtatt tcggatgcac atggccgaag catttaaaga acaggttgtc
    42781 tttcgccatc gcgtattcca gccgcggctg gtacatcacg cagctggaaa gcgaaccaag
    42841 aatgacgatc atcgccgtga tggcaacaaa gatcccagcg gtgctgccga gtgcggggat
    42901 ccaggtcagg gcgtcggaaa tcggcgtttc agaattggcg agtttgtcga agggcatcag
    42961 gccggaaatc accagcgcca gcagggtgta gagcaccaga accagcagac aggaaccaat
    43021 cagcgctcgt ggcatggttt ttccggggtt tttaatttcg ccggtcatat aacagataga
    43081 ggccatgccg gtatacgacc aactggtggc agagatcccc gccagcagcg ccataaagct
    43141 gcccgttgcg ccaatcgcag tggtggtagg ggcggcaaaa ttctccgctt taaaccagaa
    43201 gatcccaagg ccaatgacga tagtgaacgg gataattttg gcgatggtaa ttagcgtctg
    43261 aaacgctgcg ccgccttcaa cggagcgcag gtgtagcaac ataaaggcga taattaatcc
    43321 ggcggcgata aatttaccga gcaacggatc gataggcgtt aaaaagccaa gattgctgac
    43381 aatcgccagc gccataatcg acaatgacgg cgcatcgttg gcccagaagc tggcccagcc
    43441 ggagaggaaa gccagcggtc ggcttccggc atttttcaga taaacataat ctgcgccatt
    43501 ttccggataa gcggtggata gttccgcata gacgcacatt tgcgggatca caattaactc
    43561 gccaatgaca aacgcgagca ccgtaagcca cggcgtgccc gctgcttttg ccacttcacc
    43621 cacagataca aaaataccgg agccgacggt tgtcccgacg gcgattgcaa gaacggccca
    43681 aaatccgagc ttgcgttgga gttcctggct tccc
//
LOCUS       DAETQN010000018        41322 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.018, whole genome shotgun sequence.
ACCESSION   DAETQN010000018 DAETQN010000000
VERSION     DAETQN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.018"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..877)
                     /gene="nadA"
                     /locus_tag="K0W68_001057"
     CDS             complement(<1..877)
                     /gene="nadA"
                     /locus_tag="K0W68_001057"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBI3072693.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
     gene            complement(1126..1257)
                     /locus_tag="K0W68_001058"
     ncRNA           complement(1126..1257)
                     /ncRNA_class="other"
                     /locus_tag="K0W68_001058"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(1282..1357)
                     /locus_tag="K0W68_001059"
     tRNA            complement(1282..1357)
                     /locus_tag="K0W68_001059"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1322..1324),aa:Lys,seq:ttt)
     gene            complement(1391..1466)
                     /locus_tag="K0W68_001060"
     tRNA            complement(1391..1466)
                     /locus_tag="K0W68_001060"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1431..1433),aa:Lys,seq:ttt)
     gene            complement(1515..1590)
                     /locus_tag="K0W68_001061"
     tRNA            complement(1515..1590)
                     /locus_tag="K0W68_001061"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1555..1557),aa:Lys,seq:ttt)
     gene            complement(1594..1669)
                     /locus_tag="K0W68_001062"
     tRNA            complement(1594..1669)
                     /locus_tag="K0W68_001062"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1634..1636),aa:Val,seq:tac)
     gene            complement(1819..1894)
                     /locus_tag="K0W68_001063"
     tRNA            complement(1819..1894)
                     /locus_tag="K0W68_001063"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1859..1861),aa:Lys,seq:ttt)
     gene            complement(2059..2850)
                     /gene="cpoB"
                     /locus_tag="K0W68_001064"
     CDS             complement(2059..2850)
                     /gene="cpoB"
                     /locus_tag="K0W68_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBI3072694.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(2860..3381)
                     /gene="pal"
                     /locus_tag="K0W68_001065"
     CDS             complement(2860..3381)
                     /gene="pal"
                     /locus_tag="K0W68_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBI3072695.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(3416..4708)
                     /gene="tolB"
                     /locus_tag="K0W68_001066"
     CDS             complement(3416..4708)
                     /gene="tolB"
                     /locus_tag="K0W68_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBI3072696.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(4841..6073)
                     /gene="tolA"
                     /locus_tag="K0W68_001067"
     CDS             complement(4841..6073)
                     /gene="tolA"
                     /locus_tag="K0W68_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBI3072697.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(6138..6566)
                     /gene="tolR"
                     /locus_tag="K0W68_001068"
     CDS             complement(6138..6566)
                     /gene="tolR"
                     /locus_tag="K0W68_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBI3072698.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(6570..7262)
                     /gene="tolQ"
                     /locus_tag="K0W68_001069"
     CDS             complement(6570..7262)
                     /gene="tolQ"
                     /locus_tag="K0W68_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBI3072699.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(7259..7663)
                     /gene="ybgC"
                     /locus_tag="K0W68_001070"
     CDS             complement(7259..7663)
                     /gene="ybgC"
                     /locus_tag="K0W68_001070"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBI3072700.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            complement(7813..8106)
                     /gene="ybgE"
                     /locus_tag="K0W68_001071"
     CDS             complement(7813..8106)
                     /gene="ybgE"
                     /locus_tag="K0W68_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBI3072701.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(8106..8219)
                     /gene="cydX"
                     /locus_tag="K0W68_001072"
     CDS             complement(8106..8219)
                     /gene="cydX"
                     /locus_tag="K0W68_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBI3072702.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(8234..9373)
                     /gene="cydB"
                     /locus_tag="K0W68_001073"
     CDS             complement(8234..9373)
                     /gene="cydB"
                     /locus_tag="K0W68_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBI3072703.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(9389..10957)
                     /gene="cydA"
                     /locus_tag="K0W68_001074"
     CDS             complement(9389..10957)
                     /gene="cydA"
                     /locus_tag="K0W68_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBI3072704.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(11553..11780)
                     /locus_tag="K0W68_001075"
     CDS             complement(11553..11780)
                     /locus_tag="K0W68_001075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072705.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            complement(11805..14438)
                     /gene="mngB"
                     /locus_tag="K0W68_001076"
     CDS             complement(11805..14438)
                     /gene="mngB"
                     /locus_tag="K0W68_001076"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBI3072706.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(14456..16372)
                     /gene="mngA"
                     /locus_tag="K0W68_001077"
     CDS             complement(14456..16372)
                     /gene="mngA"
                     /locus_tag="K0W68_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBI3072707.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            16541..17263
                     /gene="mngR"
                     /locus_tag="K0W68_001078"
     CDS             16541..17263
                     /gene="mngR"
                     /locus_tag="K0W68_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBI3072708.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(17367..18236)
                     /gene="sucD"
                     /locus_tag="K0W68_001079"
     CDS             complement(17367..18236)
                     /gene="sucD"
                     /locus_tag="K0W68_001079"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBI3072709.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(18236..19402)
                     /gene="sucC"
                     /locus_tag="K0W68_001080"
     CDS             complement(18236..19402)
                     /gene="sucC"
                     /locus_tag="K0W68_001080"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBI3072710.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(19496..20713)
                     /gene="odhB"
                     /locus_tag="K0W68_001081"
     CDS             complement(19496..20713)
                     /gene="odhB"
                     /locus_tag="K0W68_001081"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBI3072711.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(20728..23529)
                     /gene="sucA"
                     /locus_tag="K0W68_001082"
     CDS             complement(20728..23529)
                     /gene="sucA"
                     /locus_tag="K0W68_001082"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBI3072712.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(23830..24546)
                     /gene="sdhB"
                     /locus_tag="K0W68_001083"
     CDS             complement(23830..24546)
                     /gene="sdhB"
                     /locus_tag="K0W68_001083"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBI3072713.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(24562..26328)
                     /gene="sdhA"
                     /locus_tag="K0W68_001084"
     CDS             complement(24562..26328)
                     /gene="sdhA"
                     /locus_tag="K0W68_001084"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBI3072714.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(26328..26675)
                     /gene="sdhD"
                     /locus_tag="K0W68_001085"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(26328..26675)
                     /gene="sdhD"
                     /locus_tag="K0W68_001085"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBI3072715.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(26669..27073)
                     /gene="sdhC"
                     /locus_tag="K0W68_001086"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(26669..27073)
                     /gene="sdhC"
                     /locus_tag="K0W68_001086"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBI3072716.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            27454..27561
                     /locus_tag="K0W68_001087"
     CDS             27454..27561
                     /locus_tag="K0W68_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072717.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            27767..29050
                     /gene="gltA"
                     /locus_tag="K0W68_001088"
     CDS             27767..29050
                     /gene="gltA"
                     /locus_tag="K0W68_001088"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBI3072718.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            29440..30006
                     /locus_tag="K0W68_001089"
     CDS             29440..30006
                     /locus_tag="K0W68_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI3072719.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            30057..32515
                     /locus_tag="K0W68_001090"
                     /pseudo
     CDS             30057..32515
                     /locus_tag="K0W68_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            32530..33261
                     /locus_tag="K0W68_001091"
     CDS             32530..33261
                     /locus_tag="K0W68_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBI3072720.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            33258..34319
                     /locus_tag="K0W68_001092"
     CDS             33258..34319
                     /locus_tag="K0W68_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3072721.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            34471..35517
                     /locus_tag="K0W68_001093"
     CDS             34471..35517
                     /locus_tag="K0W68_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBI3072722.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(35514..36305)
                     /gene="nei"
                     /locus_tag="K0W68_001094"
     CDS             complement(35514..36305)
                     /gene="nei"
                     /locus_tag="K0W68_001094"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBI3072723.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(36341..37075)
                     /gene="pxpA"
                     /locus_tag="K0W68_001095"
     CDS             complement(36341..37075)
                     /gene="pxpA"
                     /locus_tag="K0W68_001095"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBI3072724.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(37065..37997)
                     /gene="pxpC"
                     /locus_tag="K0W68_001096"
     CDS             complement(37065..37997)
                     /gene="pxpC"
                     /locus_tag="K0W68_001096"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HBI3072725.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(37991..38647)
                     /gene="pxpB"
                     /locus_tag="K0W68_001097"
     CDS             complement(37991..38647)
                     /gene="pxpB"
                     /locus_tag="K0W68_001097"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBI3072726.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(38670..39413)
                     /gene="ybgI"
                     /locus_tag="K0W68_001098"
     CDS             complement(38670..39413)
                     /gene="ybgI"
                     /locus_tag="K0W68_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBI3072727.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            39684..41165
                     /gene="dtpD"
                     /locus_tag="K0W68_001099"
     CDS             39684..41165
                     /gene="dtpD"
                     /locus_tag="K0W68_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBI3072728.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         9995 a        11075 c        10066 g        10186 t
ORIGIN      
        1 tgcggcaggt tgcaccctca cctgcggttg gtgcttccag taactcttta tctggcaccg
       61 cctgctgcat tttgtagaaa atgccccgat cggttgccac gataagccgc tgatgtggca
      121 atgttttcgc agcagcgatc agttgactgg tggaaccgac cgcatccgcc atatcgacaa
      181 tagcttgtgg tgattctgga tgcaccagta tggcagcatc cgggtattct tcttgcaagc
      241 gggttaacgc ctgagtctta aattcatcat gcacaataca ggcaccctgc cagcataaaa
      301 tgtctgcgcc cgtctgtttt tgcacgtaac gccccagatg tttgtcgggt gcccagatga
      361 ttttttcacc aaaactatcc agatgatcaa taagttcgac ggcaatgctt gaagttacca
      421 cccaatctgc gcgcgctttt accgcagcag aagtgttggc gtagacgacg acagtatgat
      481 cgggatgggc atcgcaaaat gcgttaaatt cttcaacagg gcagccgaga tccagtgaac
      541 attcagcctg aagtgtcagc atcagaattg ttttttccgg actgagaatt ttggcggttt
      601 ctcccataaa tctcacccca gcgactaaca acgtagaagc gggatgcttt gcaccgaagc
      661 gcgccatttc cagagaatca gaaatacagc caccggtttc ttctgccagt tgttgaattt
      721 cggggtcggt atagtagtgg gcaaccatca ccgcattacg ttcttttagc agacgtttta
      781 tcttctcgcg gtaatacgct ttttcatcaa tgcttaacgg cgtcggcttc ggggggaaag
      841 gataaatcgc cgtgtctgga tcaaacatca cgctcatctt accatctcgt tttacaggct
      901 taacgttaaa accgacatta gcgtaatatt cgctactatc ggcgtaattt gttttatatg
      961 ctaaacaaga tagtcgaatg gtggggaaaa gtcactcgaa ttacgcctaa ttccaccgac
     1021 gtagagttgc ttgcacagta agctctgacg atgtcatcag gtttacaaag tgaaattgag
     1081 gatgcctgaa tagcaaaaaa gcgcctttag ggcgcttttt tacattggtg ggtcgtgcag
     1141 gatgactcgc ttcgctcgcc cttcgggccg tcgccgcgag cggctccgtt gtttcgctgc
     1201 gctcaactcg aacctgctgc aggttcgaat ctttgaatat ctcagatagc aaaaaagcgc
     1261 ctttagggcg cttttttaca ttggtgggtc gtgcaggatt cgaacctgcg accaattgat
     1321 taaaagtcaa ctgctctacc aactgagcta acgacccctt gcgggattat gctgctttaa
     1381 tcattcagga tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac
     1441 tgctctacca actgagctaa cgacccatac gggtgttacc tgaaagattt ctactcgggg
     1501 ccaatcttta agattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
     1561 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
     1621 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
     1681 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggatga
     1741 ctcgcttcgc tcgccctacg ggccgttgct gacgcaacgt tatcctacac gcttaacatc
     1801 tgtcttcgat gctaaaactg gtgggtcgtg caggattcga acctgcgacc aattgattaa
     1861 aagtcaactg ctctaccaac tgagctaacg acccactttt tcgttgcttt cggtttgttt
     1921 gatatcccgt ggcaacggcg gcatatatta ctgatttcag acttgagcgc aacaaaaatt
     1981 tcgatgcaga tcactcaact gcttatgatt cgcacgacac gaccagaaat aatgcgactt
     2041 ctggtcgtgt gttatgcatt acatcgcgtt cagacgtttt tgtgcctgtt tagcgccatc
     2101 agtaccaggg tatttactga taacctgctg gtatacggct ttcgcttttg cggtgtcacc
     2161 tttgtcctgc atgatgacgc cgactttaaa catcgcatca gcagcctttg gtgacttcgg
     2221 atagtttttc actaccgaag caaaatagta cgccgcatca tcttttttac ccttgttgta
     2281 gtttaactga cccagccaat aattggcgtt tggcaggtaa gttgaatcag ggtaattttt
     2341 gatgaaattc tgaaatgcca ccattgcgtc atcctggcgg gatttatcct gcaccagcgc
     2401 aatagctgca ttgtaatccg tgtttgcatc gccgcttttc accggcgcgc cagcattcgc
     2461 agtaccagca tcagctgtcg gcgtcgttga tgccgccgca ccgctttgat cgctgctggt
     2521 tgattgcgcc gctgcaccac cgctgctgag gctgtcgatc tgcaacagga tctgcttttg
     2581 ccgctccacg acctgattca gttgatactg attttcctga atctgaccac gcagggaatc
     2641 aatatcggat tgattatcag aaagttgttg ctggagttgg gttaaaagct ggctgtgagc
     2701 attagaaata cgctcaagtt gagtgacgcg gtcttcgacc gagcctgagc cgacactact
     2761 gattggtgcc tgagcaaaag cggcccaggg ggccgctata ccaaccagta acgacagact
     2821 caatagttga tgtctgaagt tactgctcat gcaattctct tagtaaacca gtaccgcacg
     2881 acggtttttg gagtatgccg cttcgtcatg acccagtact gcaggttttt ctttaccgta
     2941 agaaacgatg gagatctggt ctgcagaaac gcctttaccc tgcaggtaca tcttaacggc
     3001 gttcgcacga cgttcaccca gggagatgtt gtattccgga gtaccacgtt cgtccgcgtg
     3061 accttctacg gtgactttgt aagacgggtt gctacgcagg aagtttgcat gtgcatccag
     3121 catttgagcg aagtcagaac ggatatcgta cttgtccaga tcgaagtaaa cgatgttgtt
     3181 ctgctgcagc tgttgcattt gcagacgagc ctgctcttcg gaagacatgt tgccgttgcc
     3241 gccgttcgca tccataccag tgccggcacc cagcatgcct tcgctgccgt cattgctggc
     3301 gttcttgttg gaagaacatg ccgcaattgc cataacaggc agagcaatca tcagcccttt
     3361 cagcactttg ttcagttgca tttcaatgat tcctttacta ttcaattaat tattatcaca
     3421 gatacggcga ccaggcaggg aatttgacct gtccatcagt tgccggaaga cgcgctttga
     3481 aacgcccatc tgtagaaacc aaattcagca cggatcccat cccctgagaa gagctgtaga
     3541 ttaccatagt gccgttaggt gccagacttg gcgtttcatc caggaacgtg gacgacagaa
     3601 cttgtacgcc tcccgttgcc agatcttgtt tggcaatgtg ctgctgccca ccgttggagc
     3661 tgaccattac cataaattta ccgtcgctgc tgacatccgc atcctggttc tgcgaacctt
     3721 cccaggtaat acgttgtggc gcaccgccgt tgatattcac tttataaacc tgtggacgac
     3781 cggcctggtc agaagtaaat gccaggttct ggctgtccgg gaaccaggtc ggttcggtat
     3841 tgttactgcg accatcagtc acctggcgga tctgaccaga agccaaatcc attacgtaca
     3901 ggttcaggct accggttttc gacaaggcga atgccagttt gctgccgtct ggcgagaatg
     3961 caggcgcacc gttgtgacgc gggaatgaag ccacctgacg tacagcgccg tttgccagcg
     4021 tttggataac tagcgcggaa cgaccgcttt cgaaggtcac ataagccagt ttagaaccgt
     4081 ctggtgacca cgccggagac atcagcggct gtggtgaacg gtgaacgaca aactggttgt
     4141 aaccgtcata gtcagatacg cgcagttcat acgggaactg accgccgttg gtctgaacaa
     4201 cgtaggcaat acgggtacgg aacgcacctt taatgccggt cagtttttca aacacttcat
     4261 cactggcggt atgaccagca tagcgcagcc actgcttgtt cactttgtac gagttctgag
     4321 caagtacagt acccggtgcg ccgccagtgt caacaagttg ataagcaaca ttgtaagaac
     4381 catccggatt cggagtgacc tgaccgacaa ctacagcgtc aatacccagc gcggaccatg
     4441 cagctggttg tacttcctgc gcactacccg gctgctgtgg cagacgagcg cgatctaacg
     4501 gattaaattt accgctgtta cgcaagtcgg cagcaacgat gccgccaata tcttcaggtg
     4561 ccgcaccagg gcccgcccac tggaaaggaa caacaccaat aggacgaccg gaatctacac
     4621 cgctgtcgat cacaatgcgg acttcagcat gcagaactga tgcccacagt atgagaaaac
     4681 caaatgctac tcgtaatgcc tgcttcatca tatctccctt atctggaccg atggcccacg
     4741 ataatttagc agaatgttaa caaactaaaa tacacaaaac taccagaacc ccgtggcaac
     4801 cggtgcctga tgttgaccgt cggaacagtc aacatcgcga ttacggtttg aagtccaatg
     4861 gcgcgttttt gaacacttca tatactgcct ggcttggtgg tttcgggatc ttcgcaagtt
     4921 tagctgctgc caacgcagcc tgacaaagtg cgggatcgcc accttcaggt ttgatatcca
     4981 gtaacatacc atcgggtgcc agttttatgc gcagcgtaca ggttttgcct gcataggacg
     5041 atgcgtcata gaacttactt tcgatagcag atttaatctg cccggcatag ttattgatat
     5101 cggcccctga tgcgccattg tttttagtat taccactccc ggcagacgaa gcattgttcc
     5161 ctttcgcccc tccccccgtt ttcggtgcat tcttaccaga gcttagctca ccgaaaatat
     5221 catctgcctc tgcggcagct tttgctgcag cggctttttc agctgctgct tttgccgctg
     5281 ccgctttttt gtctgcggcg gctttttctg ccgctgcttt tgccgctgcc gcttttttgt
     5341 ctgcggcggc tttttctgcc gctgctttct tatcagctgc tgccttatca gcagccgctt
     5401 tttctgctgc ttttttgtcg gctgcagctt tctctgctgc cgctttcttt cttgcttcag
     5461 ctgcagcagc ttctgccgct tccgctttct tcttcagtgc cgcagcggct gcctcggctt
     5521 ttttctgcgc ttcggctgcg gctttggcgg cttctgcttc tgcttttttc ttcgcgtctg
     5581 ccgccgcttt cttcgctgct tcttccgcag ctttagcatc cgcttcggcc ttcgctttag
     5641 catctgccgc cgctttcgct gccgcctctt ccgcttgctt ctgctttaac tcggcctgtt
     5701 ttgcggcttc ttcagcctgc tttttctgtt cctgagcagc taaccgctct ttctcaagtt
     5761 gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag ttcttcagca gcctgctgtt
     5821 cctttatctt gcgctgctca tcagaacgct tcgcgcttga ttcctggctt tgcatgcgtt
     5881 tgtactgctc aactaccgca cctgaatcaa ccatgacagc gtcgatggac gaaccaccgc
     5941 cgcctccggc tgaagcttct atattctcat cgaacgaact ccagagcagc gccgcaaata
     6001 agatgacatg cagcactgct gaaataatta tcgcccgctt gagcttgtcg ttttgttcgg
     6061 ttgcctttga cactctcggt ttccaaaaac tgttcgcctg ttacccgctc tctttcaagc
     6121 aagggaaacg cagatgttta gataggctgc gtcattaaac caaccgattt cacacccgca
     6181 ctatgtaaca agttcagtgc tttaattatt tcatcgtaag gcacatcttt tgcgccaccg
     6241 atcagaaaga ccgttttcgg gttggccttg aaacggctgg acacttccgc caccacctgc
     6301 tctggtggta aacgctccag gcgatctttc tcaaccacca cggtgtactg accaatacca
     6361 gacacttcaa caatcactgg cggattatcg ttactgctca ccgcctgtga ttcagtagcg
     6421 tctggcagat cgacctccac gctctgggtg atgatgggcg ctgtcgccat aaagatcagc
     6481 aacagcacca gcagtacgtc cagcaacggt acaatgttga tttcggactt gagatcgcga
     6541 cgacctcgtc cacgcgctct ggccatggct taccccttgt tgctctcgct aacggtaaac
     6601 gcctggcggt gcagaatcgc ggtaaactct tccataaagt tgtcgtaatt cagttccagt
     6661 ttgtttacgc gctggttgag gcggttataa gccattaccg ccggaattgc ggcgaacaga
     6721 ccaattgcag tcgcaatcaa cgcttctgcg ataccgggcg caaccatttg cagtgttgct
     6781 tgttttaccg ccccgagggc gataaaggcg tgcatgatcc cccagaccgt accaaacaga
     6841 ccaatatacg ggctgatgga gccaaccgta ccgaggaacg gaatgtgcgt ttccagattt
     6901 tcaagttcac ggttcatgga gatacgcata gcacgcgacg ccccttccac tacggcttcc
     6961 ggcgcatggc tattggcacg atgcaggcgc acaaactctt tgaacccgct gtaaaatatt
     7021 tgttccgaac cagtcagatt atcccgtttc ccctgactct cttgatagag gcgagagagt
     7081 tcgattccag accagaattt atcttcaaac gcttcggctt cgcgcgccgc tgcgttaaga
     7141 atacgggtcc gctggataat aatggcccag gatgcgattg aaaaaccaat caaaatcaac
     7201 atgataagtt taaccagaag gctagccttc aggaacaaat caaggatatt catgtcagtc
     7261 actgcttaaa ctccgcgaca atagacttgg gaagcgcaca aggcttcatt ttgagtgggt
     7321 caacgcaaac aaccagaacc tctgcttcat tcagcaaagt attctcggcg ttgacaatac
     7381 gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat ttcagtctgt atttcgagca
     7441 tatcgtcgag ccgcgcaggt gcgtaatatt ccaccgtcat tttacgtacc acaaaggcaa
     7501 cgcgttcagc catcagcgcc tgctgactga agtgatgatg acgcagcatc tctgtgcgtg
     7561 ctctttcata aaaagcgacg taactggcgt ggtacaccac accaccggca tcggtatctt
     7621 catagtagac gcgaaccggc catcgaaaca gcgttgtatt cactttacat cccggtaatg
     7681 caacaaaagt tagagctttt aaacgctgct actatacgcg cgcgattgag gtttgggaat
     7741 gagggcaagt taagggagcg taaaaattta tgggcctgga gcggcccata attttttaaa
     7801 gaaaattctg atttaaaaga agaagaaaat cagcccgaca atgagaacaa tatcggcaag
     7861 caatgggcaa aaaatccctt gccaaagaac cttctgcgga cgaaagccca cgccgtgaat
     7921 cacaccagca cagacggccc acatcagcaa taaaccatgc cagatttcca gttcactggt
     7981 cttcgcggca aaacgagatg ggtcccaaaa catacatcct gctaacagaa gcgccatcac
     8041 gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca tacaaggtcg cgataatctt
     8101 actcatcaga tgtcttcttg accggctttg cctgattcga cgtgttcaag cgccagcgcg
     8161 gtgattaccc caaacgaaca ggcaagaagc gttcccagaa tccatgcgaa ataccacatt
     8221 tttagctcct tacttagtac agagagtggg tgttacgttc aatatcttct ttggtgatac
     8281 gaccgaacat tttccagtaa caccaagcgg tgtagagcag aatgatcggt accagaacca
     8341 ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga agttgcatcc cacattgtca
     8401 gacttgcgtt catcatggtg ctggacggca tcacaaacgg gaacattgcg ataccggctg
     8461 tcaggatgat gcaggccagc gtcagggagg agaacacaaa cgcccacgcg gctttatcca
     8521 tacgtgcagt caggatggtc agcagcggaa gaaccacacc cagtgccgga atagcccaca
     8581 gaattggcgt gttgttgaag ttaaccagcc atgcgccagc ttcacgaacc acttctttat
     8641 tcagtgggtt agaggctgcg taatggtcca ttgtcgattt cacgacataa ccatcgatac
     8701 cgtacatcac ccatacgcca gccagtgcga aacagaccag tgtcaccagc gcagccacct
     8761 gagccgttgc acgggtacgc aggtgcagtt cgcccacggt acgcatttgc agataggttg
     8821 cgccctgagt aatgatcatc cctacgctca ccacgcctgc cagcaggccg aacgggttaa
     8881 gcaactggaa gaagttaccg gtgtagtaca gacgcagata ttcatcaacg ttgaacggta
     8941 cgccctgcaa caggttaccg aacgctacac caattaccag cggcggaacg aaactaccaa
     9001 tgaagatgcc ccagtcccac atgttacgcc agcgggtttc ttcaatcttg gagcggtagt
     9061 caaaaccgac cggacggaag aacaaagacg ccagcacgag gatcatcgcc acatagaagc
     9121 cggagaacgc agcggcatag accatcggcc aggcagcaaa gagtgcgccg cccgcggtga
     9181 tcagccaaac ctggttaccg tcccagtgtg gtgcaatgga gttaatcata attcgacgct
     9241 cggtgtcgtt acgaccgagg aaacgggtga gcatgcccac ccccatgtcg aaaccgtcag
     9301 tgactgcaaa accaatcagc agaacgccaa ccagcagcca ccagataaaa cgcaatactt
     9361 cataatcgat catttgacga ctcctgtctt agcgtgccgg ctgagtagtc gtggaagact
     9421 gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc gaggcgtgca aacttgaaca
     9481 ttaagaacaa ttctgccacc aggaacaggg tatacaggcc gcaaatcagc accattgaga
     9541 agatgagatc gcctgcggtc agtgacgagt tcgccacagc tgtcggcagc acttcaccga
     9601 tagcccacgg ttggcggcca tattcagcca cgaaccagcc cgcttctaca gcaatccacg
     9661 gcagcggaat accgtacagc gcggcgcgca gaagccattt tttctcgcca atgcggttgc
     9721 ggatgacact ccagaaagag agcgcgatga ttgccagaag caggaagcca cacgccacca
     9781 tgatacggaa cgcaaagtac agcggcgcta cacgcgggat ggagtctttg gttgcctgtt
     9841 gaatctgcgc ttcagtcgca tcagccacgt ttggcgtata gcgtttcagc agcagaccgt
     9901 aaccgaggtc tttcttcatg ctattgaact ggtcacgaac cgcctggtcg gtagaaccag
     9961 aacgcagttg ttcgagcaga gagtacgcct tcatcccgtt acgaatgcgt tcttcatgct
    10021 gcaccatcag ctctttcagg ccgataaccg gggtatccac ggaacgcgtt gcaatgatgc
    10081 ccagtgcgta agggatctga atcgcaaatt tgttcgtctc ctcttcctga tcaggaatgc
    10141 cgaacagagt aaaggcagca ggcgcaggtt gcgtttccca ctcggcttca atagcagcca
    10201 gtttggtttt ctgcacgtcg cccatttcgt agccggattc atcacccaga acaataacag
    10261 acagaacagc agccataccg aagctggcag cgatagcaaa ggagcgttta gcgaaggcga
    10321 agtcacgacc tttcagcata taccatgcgc tgataccgag gatgaacatc gcgccagtca
    10381 cataaccaga cgctacagtg tgaacgaatt tcacctgagc aaccgggtta agcaccagct
    10441 cggagaagct caccatctcc atacgcatag tttcaaagtt gaaatcggac gcgattgggt
    10501 tttgcatcca gccgttcgca accagaatcc acagtgcgga caggtttgaa ccgagcgcca
    10561 ccagccaggt gacacacata tgctgaactt tacccagacg atcccaaccg aagaagaaca
    10621 gacctacaaa ggtggattcg aggaagaagg ccatcagacc ttcgattgcc agcggcgcac
    10681 cgaagatatc ccctacatag tgggaatagt aagaccagtt agtcccgaac tggaactcca
    10741 tggtcagacc ggtagccaca cccagagcga agttgatacc aaacaacttg ccccagaact
    10801 tggtcatatc tttataaatc tgtttgccgg agaggacgta gaccgtttcc ataatggcca
    10861 gcaggaacgc cataccgagc gtcagtggca caaaaaggaa gtggtacatc gcggtcaagg
    10921 caaactgtaa gcgcgacagt tcgactatat ctaacatcat gactccttgc tcatcgcatg
    10981 aagactccga gagtgaaccc cgttagaaag ggtcacacgc atgccccaat agaaatttgt
    11041 ttgaacactt tcatcatacc atgtgaatgc acacaaaaac ggcgtaaaga aaggttgcaa
    11101 acatgttaat aaaaactcaa attgatccca cgtatatatt acgctgcaaa atccttacaa
    11161 taaacaggtt tttgttgaag caaatttgca tttttcgacg gtgatcaatt tatagctaaa
    11221 ttaccgcctt tcagccaatt tgatcgagaa caatttatct ctttttgatg ccaatttcca
    11281 agacttatac attgataaat atcaattcct acaaattatg ttaatcatgt ttcaataagg
    11341 taactttagt gacatttatg tttaaaatgt gtgagttata aggctgaaat tagtttcggt
    11401 tggcaattta aagataaaaa gagacgaagc aaaaacagca cggttgacct taattctttt
    11461 tttggtcact ggctaatacg acgcagagtt tcaggattac taataaaggc ataaatccac
    11521 cccgccgata ttaacattta attaacttgt aattatatta taattaactc acccagggtt
    11581 attattttgt taaaaataaa gccacatata cgtggcttga gagtgaagat aaacgcaaaa
    11641 ttacctgatg cgctacgctt gccagtgtct acatcatctc tgcaatatat tgaatttgcc
    11701 tgcttttgta gaccgaataa agcgttcatg ccgtatccgg catgaacaaa gcgccctttg
    11761 tcaacaatct ggccgcgcat atgtggcttc ggtttgattg ctattcaggc aagccggtaa
    11821 ctgaacgtcc gtgactggcc gggtaaaaaa ggccccgata gatttgaacc ttgattttcc
    11881 tcggtggtaa tgtgttcatc catcatcgtt tctgagcaag aaatcacctc gcgactgaaa
    11941 gcaacagtcg catcacaggt tgctgattca gccggattaa acagccgtaa aatcacttct
    12001 tgtcggtctt cagctttctt aagtgcgctt atcaggcatc ccactggggg cattttcaac
    12061 aaactataac tttccggcac gttgaatccg gctttgttga gcttcatcgc atcccatggg
    12121 attttgttgt aacactgtac tggagtcagc catgctcgcg cctgctgagc tacaccagcg
    12181 gcggttggcg taccggtata actcaataaa cttaggcgac aagaaagcag accacgtagt
    12241 tgtgagtccg ggactggcat tttaattccc gaaggccgcc caggccttaa aagcagatct
    12301 tctttgccca gtaagcccac gccacgcagc aacgtaatgg caaaggtttt cttctcttca
    12361 ccgatgactt caaattcacg taacccttcg ctaaagacag ccatgccgtt acgcccttcc
    12421 tgtaaggcaa catagttgag catattccat accggaaccg gcgcttcttt ccagccttct
    12481 tgctgccagt tgttcattgc actgtcgttc acggggcgcg ttagcgaacc aaactgcgta
    12541 tctgccagaa cactgtcagt gttaaaaggt gtagggacca ggacacgaag gcgatgatcg
    12601 tcagcctggt tatcaaggtt gatatccaca tcaatacgcc tgctgttatg actaagagtg
    12661 acaaccaaca ccccccctac tctgccagtg gattgccggg cgctgcgttc tgacaaattg
    12721 agcggcactg ccatgtcata gcggataaca gccctgctct gccaggcttc atgaataata
    12781 tcgcattgcg gtttcgcgtt cgctgcggta attacccact cttcttttgc gggtgaatag
    12841 tcatattcat caccatcatc agagctttct tcaatttgca atacccggtc atagcgcaca
    12901 ccgctgtctt tatctaccag ttgcagagaa ccatcctcat tgagcgcaat ctgccagaaa
    12961 gcattttcca gaatcccttc agcgtcactt tttgccgcaa tcacgttgcc aggctgattc
    13021 gcttcaatat aaagcgtgcg atagcccata gaagggacaa tctggttgat ctgaatatca
    13081 aactccataa agggatcata attgccataa tgaacaattt gccgatcgat taggccagga
    13141 tcgatctcac gggcatggcg aataaaatac ggtacaggct gaccgcgatc gtcttgtaga
    13201 ttaaactggc tggcgcgcag ccgcacagtg gtgttaataa cttcttcccg cggccagggc
    13261 atcaggttaa acagcacgag tttgtcggca tcactctgtg gcatgttatc ggcaattttg
    13321 cgcatgtaga aacgcagcag attatccgcc atgtcttcag ccagttcgaa gcgggcgaca
    13381 atttcgcgat gaactttgtc actgcaacaa caaccgatac tgtcgtgggc atgatttttt
    13441 aagatctctt tccacatttt ctccagcaag ccgtggtggt attcaaaacc caacgtccag
    13501 gccagcgttg ccagcggttc cagcagatta acaatcttat tttcaatacg tgcgtgggca
    13561 attttgatat ccatgcgcgt ggaaccgatg gtgcgatgta cgcgcatata tttgccatcg
    13621 ataaattcac ctttcagggt ggccagactt tcacgctgcg cttcgatctt ctcaaatacc
    13681 tcttcaaagc ggctcatcac aaacttacgt tgagggtaga tctcacgtag cttatccatc
    13741 acttcgaaga tattttgctg caatggcatc tggtcatgac cattcggcag caaaatctct
    13801 ttggttacag acgctttttc cagcacgtca aaataactgt cgaggcgttt acgtaatccg
    13861 ttttcgtcgg caggtaagta cttaccgatg gcgtagccca gcggcagcac ctgcgccgtc
    13921 acttcgctac cgtcactgct ttgccacaaa aactcggttt tatcagtacc gtggcgctcc
    13981 gaacatccgc gccagaacat agtgcgggta atgccaaatc cattgtaaat atgcggcagt
    14041 tgcccggaca tgccaaagga atccggtaaa taacctattt tcatcggctc gccaaacgcg
    14101 aggcagtcac gcattccgta catcagatta cggacgatgg attccgcaga aacaatcgtg
    14161 gtatcggtct gggtatacca ggggccgata atcaacttac cggcttctac ctgtttcttc
    14221 acgcggtctt tgttttccgg tttcaccgcg aaataatctt cgaggatcgc cgtttgcccg
    14281 tcgagtacgt aatatttgta ttcgttgtcc tgttccagtc ggcacaggat ctcttccata
    14341 ttattgacca gcagaatgcg tgactcttcg gtggtgaaat accactcccg atcccaatgc
    14401 atatgcgggg tgatgtgaac gcgagatacc gctttcatcg tcgtttcctt tttgtttatg
    14461 gcattacgcc atcagtcaga tagttgccat gcttaaccgc gtgacgccgc cagatcagga
    14521 gaattgcagt cgagattgca gcccccacca atgccgcacc aaaccagcca attgccgcca
    14581 taacaccgcc cgcgccatta tcatgaagta aaaagagcga gaaaatgccg gcaccgggtg
    14641 tcgaaaggcc gatattcatc gcaccgacaa tagcgcccgt taccatagag cccagcacaa
    14701 acgaaccaat aacccgcagc ggatcttcaa tcgccatcgg gatcgcccct tcggtaatac
    14761 ctgccagccc taacagccag gtggatttcc cggtctcaat ttcaaactct ttaaacaggc
    14821 gcggtgcgag catcgtggaa gcggttacgg taaatgccga aaccattttg acggaggcga
    14881 aaatggcata cgggccgtaa acgccgttcg ccattgcgcc caggcagaat gcataagcgg
    14941 ctttgttcac tggccctcca aggtcaaagg aacacataaa accgagaatg gcacccagca
    15001 acagcgcgtt acttcctgac agaccgttca gccaggcggt aagcgagtta ttgatccatg
    15061 cgacaggttc tcccaccaca aacagcatca gactgcccgc tcccaacgta ccgagcaccg
    15121 ggtagagata aaaagtcagg aatccattga atttactgct aagacgcaag tgatttttca
    15181 cccagcgcat caagtaaccg gctatcaatc cgccaacgac cgcgccgaga aacccggagc
    15241 cgatcatgtt ggcggcaagt ccagccgcaa agcctggcgc taacgccggt ttatctgcca
    15301 gagaataggt ggtataggcc gcgagcaccg gtaccatcaa aattccgagc agcccgccgc
    15361 ccagcttgcg gtacatccac agccaggagt tttcttcatt aaacagatct tgtagcccga
    15421 agatttgcga cagtaatacc gcgaccgcca gcaccgtgcc ccccgcgaca atcaacggta
    15481 cggcaaaaga gattccgctc aacagtgcct gtttcagctc cgttttgaca cttttcaccg
    15541 gttgcgtatc ttgctgtacg gtacgcgtct catcgctacg cttgagggta agcgcttgtt
    15601 ggatcaacgc ttctgcatgg cgaatcggct cggcaacagg cactgaaagc gcgggaatgc
    15661 cattaaaacg ctcactctcc ttgatggcga cttcagccgc aaaaatacag gcggttgcac
    15721 tattgagttg atccgccgtt aaacgccctt caatgccgtt agcgccttgt ttttcaacgt
    15781 aaacatttac gccgagtttg cgtccggctt tttccagata ttccgcagcc atataggtgt
    15841 gagcaatacc cgccggacag gccgttacgc agacgatagt tggcgcgttg gaaaaagagg
    15901 cagaaggttg cgtgcctccc ttgtcatcca gcgccgagag caactcatca ggcgtcgtcg
    15961 ccgactgtat acgcgcccga atctcatcat ccgcaaggcg cgtggtcagc gctgtcagca
    16021 gttgcatatg cgtagtaccc gcttcattgg ggggaatcgc cagcagcacc actaaatcaa
    16081 ctgcttccgg gccatcaacg ccttcccact gaagcggctc gctgagtgtg gcgaccgcaa
    16141 acgccgcttc ttttaccgca gcagttttgc catgcggcac agccaaccct tcacctaagg
    16201 cggtcgggcc aaggctttca cggcgataca cttcttccag aaattgctca gtactggaaa
    16261 ttttccccag agcagcaaga cgttgagtca acgcgtggat ggcctcttca cggctggtaa
    16321 agcgcgcatt cagacacaac gcatcgcggt gggtcagagt cgtcagattc atcatgatcc
    16381 tcacttgatc gtttttatag tcgcgccagt gtgcccgata aaacaatacc atcatgtgat
    16441 caatttcact tgattgaaat tatttgtatt gtatttgtat tatttacagg atgaatttat
    16501 cgggcataat ggctaaattc atattaatga gatactaaaa atgggacaca agcccttata
    16561 ccggcagatt gccgatcgca ttcgtgaaca aattgcgcgt ggcgagctta aacctggcga
    16621 tgcgttaccc actgaatcag cgctacaaac agagtttggc gtcagccggg ttacggtgcg
    16681 tcaggctttg cgccagctag tcgagcagca gatcctcgaa agcattcagg gcagcgggac
    16741 ttacgtcaaa gaagagcggg tcaattacga tatttttcag ttaaccagtt ttgatgaaaa
    16801 actgtcggac cgtcacgtcg atacacacag tgaaatcctg atatttgaag tgattccggc
    16861 tgacgatttt cttcagcaac agctacaaat caccgcgcag gatcgtgtgt ggcatgtgaa
    16921 gcgcgtgcgc tatcgcaagc aaaagccaat ggcgctggaa gaaacctgga tgccgcttgc
    16981 gttgttcccg gatctcacct ggcaggtcat ggaaaattcg aaatatcact ttattgaaga
    17041 agtgaagaag atggttatcg atcgtagcga acaggaaatc attcctctga tgccaaccga
    17101 agagatgagc cgtctgctta atatcagcca gacaaagccg attcttgaaa aagtatcgcg
    17161 tggatattta gttgatggtc gggtgtttga atatagccgc aacgctttta ataccgatga
    17221 ctataaattt accctgatag cgcagcgaaa atcatcgcga taagcacaaa aaaggccatc
    17281 atacgatggc caaccatgtc gaaaccgaca gcccttcgcc attcctgtag agggaagcgg
    17341 cgagggctat ttcttattac agatatttat ttcagaacag ttttcagtgc ttcaccgata
    17401 tccgccaggc tgcgaacggt tttcacgcct gcggcttcca gagcagcgaa tttctcatcc
    17461 gcagtccctt tcccaccagc aatgatcgca cccgcgtggc ccatacgttt gcctttcggc
    17521 gcagtcacac cagcgatgta acctacaact ggcttggtaa cgtgctcttt gatgtacgct
    17581 gccgcttctt cttcagcgct accgccgatc tcaccgatca tcacgatcgc ttcggtctgc
    17641 ggatcttttt cgaacatttc gaggatgtcg ataaagttag agcctgggat cgggtcaccg
    17701 ccgataccaa cacaggtcga ctgaccgaaa ccgtaatccg tggtctgttt aaccgcttca
    17761 taggtcagtg taccggaacg ggaaacgata cccactttac ccggtttgtg aatgtgaccc
    17821 ggctggatac cgattttgca ttcacccgga gtgataacgc ccgggcagtt cgggccgatc
    17881 atacgaacgc cggcttcgtc cagtttcact ttcacagtca gcatatccag cgtcgggatg
    17941 ccttcagtga tggtgataat cagtttgatg cctgcatcaa tagcttccag aatggagtct
    18001 ttgcagaacg gtgctggtac gtagataaca gaagcggtag cgccagtagc agcaacagct
    18061 tcacgcacgg tgttgaacac cggcaggccg aggtgggtag taccgccttt acctggggtt
    18121 acgccgccaa ccattttagt gccgtatgca atggcctgtt ctgagtggaa agtcccctgg
    18181 ctaccagtaa agccctggca gataaccttg gtgtttttat caattaaaat ggacattatt
    18241 tcccctccac tgcggcaaca acctgctgag ctgcatccgt cagacctttt gctgcaataa
    18301 tattcaggcc gctgtcagcc agtttcttcg cgccgagttc ggcgttgtta ccttccagac
    18361 gtaccacgac cggtacgtta acacccactt ctgctaccgc gccgatgata ccgtcagcga
    18421 tcaggtcgca acgaacgata ccgccgaaga tgttaaccag aacggctttc actttgtcgt
    18481 cagagaggat gattttgaac gcttcggtta cacgttcttt ggttgcgccg ccgccaacgt
    18541 caaggaagtt agccggttcg ccgccgtgca gtttaacgat gtccatcgta cccatcgcca
    18601 gacctgcgcc gttaaccata caaccgatgt taccgtccag cgcaacgtag ttcagttccc
    18661 actgtgcagc ctgtgcttca cgcggatctt cctgcgactg gtcacgcatt tcgcgcagat
    18721 caggctggcg gaacagtgcg ttgccgtcag cgcccagttt gccgtcgagg caaatcagat
    18781 cgccctgttt ggtgatgacc agcgggttga tttcgatcaa cgccaggtcg cgctccagga
    18841 aaatggtcgc caggcccatg aagattttgg tgaactgctg aaccagttta ccttccagac
    18901 ccagtttgaa cgccagctcg cgtccctgat acggcatcgg gccagtcagc ggatcaagcg
    18961 caactttgtg gatcaggtgc ggagtttctt ccgccacttt ttcgatttcc acgccgcctt
    19021 cggtggaggc cataaagacc acacgacggg aactacggtc tacaacggca ccgagataca
    19081 gctctttagc gatatcggtc gctgcttcaa ccagaatctg gttaaccggt tggccattgg
    19141 catctgtttg atacgttacc agacgcttgc cgagccagtt ttctgcaaaa gcacggatgt
    19201 cttctttgct gtttacaact ttcacaccgc ccgctttacc gcggccacca gcgtgaacct
    19261 gacatttcac tacccacggg ccggcaccga tttttgaagc ggcttcttct gcttcgcgcg
    19321 gagtagtaca ggcataaccc accggtgctg gtaagccata gcgggcaaaa agttgttttg
    19381 cctgatattc atgtaagttc atgtgttctg tccatccttc agtaatcgtt atcttttaaa
    19441 ccgtagaccg acaccctatc cacgtcggtc tacagtgcag gtgaagctta aactactaca
    19501 cgtccagcag cagacgcgtc ggatcttcca gcaactcttt gatcgttacc aggaagccca
    19561 cggattcgcg accatcgatc agacggtgat cgtaggacag cgccaggtac atcatcggca
    19621 ggatctcaac ctgaccattc accgccatcg gacgatcttt gatagcgtgc atacccagaa
    19681 ttgcgctctg cggcgggttg atgatcggcg tagacatcag ggaaccgaac acaccaccgt
    19741 tggtgatggt gaagttacca ccggtcagat cttcaaccgt cagcttgccg tcacggcctt
    19801 taactgccag ctctttgatt ttcttctcga tgtctgccat gccgagggta tcgacatcac
    19861 gcagtaccgg cgtcaccagg ccgcgcggcg tggaaaccgc catgctgacg tcgaaatagt
    19921 tgtggtaaac cacgtcatca ccgtcgatag aagcattcac ttccgggtaa cgtttcaggg
    19981 cttcaaccac cgctttcacg tagaaggaca taaagcccag acggatgccg tggcgttttt
    20041 caaacgcttc accgtactgc ttacgcagat ccataatcgg cttcatgttg acttcgttga
    20101 acgtggtcag catggcggtg gagtttttcg cttccagcag acgctctgcc acacgcttac
    20161 gcaggcgagt catcgggaca cgtttttcac tacgtgcagc cagagccggt tgcgccgccg
    20221 gagcagccgc tgccggagca gactctttcg ccggggcttt cgccagatgt ttttccacat
    20281 cttcacgagt cagacgacca cccacaccgg tgcctttaat ggcgctggcg tcgagattgt
    20341 gttcagccag caggcgacgg atcgccgggc ttaacgcatc gttgttttgc tcttccagag
    20401 acgcctgctg gcgttgcgcc ggagtggacg ctttctcttc agatttggcg ctggtttctt
    20461 taccggcgct gttgccttca cgcaggcgac caaggatctg acgagacgtt accgttgtac
    20521 cttcatcttc cagaaccgca tccagaatgc cgtctgctga tgccggtact tccagtacca
    20581 ctttgtcagt ttcgatttct accagcactt catcacgtac gactgcgtcg ccgggttttt
    20641 tatgccaggt tgcgacggtg gcatcggcta cggattcagg caggtcaggg accagaatat
    20701 ctacgctact cattgtgtat cctttattta ttcgacgttc agcgcgtcat taaccagatc
    20761 ttgttgctgt ttctggtgaa cggacatata ccctaccgcc ggagaggcgg aggccgtgcg
    20821 gcctgcataa cgcagagaag ccccaaacgg aatcacttca cggaaatgat gctggctgca
    20881 gtaccatgcg ccctggttga gcggctcttc ctggcaccag acaaaatcct tgacgtgagc
    20941 aaactgctgc aacacttcct gcatcgcttt atgcgggaac gggtagagtt gctcgatacg
    21001 cacaatggcg acatcgtgtt gattgttctt acgacgctgt tccagcaggt cgtaataaac
    21061 cttaccagaa cacatcacta cgcgcttcac gcccttcgga tcaagctcgt cgatttcacc
    21121 gatggctggc aggaaggtgc cgttcgccag ttcttcgagg ctggaaaccg ccagcggatg
    21181 acgcagcagg gatttcggcg acatcactac cagcggacga cgcatcccgc gcagcgcctg
    21241 acgacgcagc atgtggtaaa cctgtgccgg ggtagacgga acgcaaacct gcatgttttg
    21301 ctcagcacaa agttgcagat aacgttccag acgcgcggag gagtgctccg gcccctgccc
    21361 ttcgtaaccg tgcggcagca acatcaccag accacacatc cggccccatt tctgttcgcc
    21421 agaagagatg aactggtcga taaccacctg cgcaccgttg gcgaagtcac cgaactgcgc
    21481 ttcccagatg gtcagagtgc gtggttctgc ggtggcataa ccgtattcaa acgccagcac
    21541 tgcttcttca gacagtacgg agtcccagac gcggaacgcg ccctgcccgt tatggatgtg
    21601 ttgcaacggc gtgtaagtgg aaccgttaga ctggttgtgg atcaccgcgt ggcggtggaa
    21661 gaaggtaccg cgaccggagt cttcacccga caggcgaacc ggaatgcctt cgtcaaccag
    21721 cgtggcgtaa gcgaggtttt ccgcaccgcc ccagtcgaac agtttctcac cggcagccat
    21781 cgcctggcga tcgccataaa tcttggcaac gcgagactgc atttcaactg cttccggcac
    21841 cgtgctgatg cgtttcgcca gctcctgcag gcgcttcatc tcaactttgt tcgggtactc
    21901 ttcgtcccat tcgtggttga ggtacggcga ccaggtgaaa gagtgcatgt tcatcggacg
    21961 ccactctgcc actacgcaat cgccagcatc cagcgcatcg cggtacaggt taaccatctc
    22021 ggtggcatct tccagcgtcg ccactttttc ctgctccagc ttgtcagcgt agattttgcg
    22081 cggtgtcgga tgttttttga ttttctgata catcagcggc tgggttgcgc tcggctcgtc
    22141 ggcttcgttg tggccgtgac ggcggtagca caccaggtcg atgaagacat cacgtttaaa
    22201 ggtgttacgg aaatcgagcg ccagacgggt cacaaaggca acggcttccg gatcatccgc
    22261 gttaacgtgg aaaatcggtg cctgaaccat cttaccgata tcagtacagt acggtgtaga
    22321 acgagcatcc agtggattag aggtggtgaa accaacctgg ttgttgataa cgatacgtac
    22381 cgtaccgcca acttcataac cacgcgcttt cgacatgttc agagtttcct gaaccacgcc
    22441 ctgcccggtc actgcggcgt caccgtggat ggtgattggc agcactttgt tgctgctcgg
    22501 ctcatcaagt ctgtccagac gggcacgaac agaaccgata actaccgggc ttacaatctc
    22561 aaggtgagac gggttaaacg ccagcgccag gtgcaccagg ccgccatcgg tctggaagtc
    22621 agacgagaag cccatgtggt atttcacgtc acccgtgccg aggtgttctt tatgtttacc
    22681 ggcaaactcg tcgaacaagt cttgcggttt tttacccagc acgttcacca gcacgttcag
    22741 acgaccacgg tgcgccatcc caagaaccac ttcgcgggtg ccgctgttgc cagcgtggcg
    22801 gatcatctct ttaagcatcg ggattaacgc gtcaccgcct tccagcgaga agcgttttgc
    22861 gccagggaat tttgcgccga ggtaacgttc aaggccttca gcggcggtca gttcgcttaa
    22921 gaagcgtttt ttctcttcgc tattgaaagt cgcgcgacca gactcgatac gctgttggat
    22981 ccagcgtttt tcttcggtgc tggtaatgtg catatactcg gcaccaatcg ggccacagta
    23041 ggtttgcttg agggcttcca gcagctcgcc gagtttcatg ttttctttgc cgctggcaaa
    23101 tgaaccgacg ttgaaggtct cctggaagtc tgcttcggtc agatcgtgga aagacggatc
    23161 cagatcggcc actttatctt gctgccacag tcccagcgga tcgagattcg catgctggtg
    23221 accacggaag cggtatgcgt taatgagctg caggacttta acctgcttca cattggtgtc
    23281 agggtcggag atcgttgaag agtaacgtga agcgtctttc gccaggcggc ggaaatattc
    23341 acgcgtttga gagtggaatt gatccggttt gactcccgta ccaggtaact gctggaacgt
    23401 cgaacgccag ttagcgtcaa ccgagtcagg atcggttaag aagtcttcat agagctgttc
    23461 tatccagctc tggtttgcgc cagagaggta agaagagtcc aaccaggctt tcaaagcgct
    23521 gttctgcatc gtgatccctt aagcatcttt tttatgctta cttcgccgtg gatactacca
    23581 cgcacagtga tgtgcgtgcc ggggttcact tgcgagctct tgtctatgta gtggctcgcg
    23641 aaggaacctt taaaaactgt cttagtgtca ggggcagttt ttaaagattt cctgcatttc
    23701 tttgttacgc ctgatgcgct gcgcttatca ggcctacggg taatgacttg taggccggat
    23761 aaggcgttca cgccgcatcc ggcactggtt gcctgatgcg acgcttgcgc gtcttatcag
    23821 gcctacggtt tacgcattac gttgcaacaa catcgactta atatggccga tggcgcgcgt
    23881 cgggttcagc cccttcggac atacactgac gcagttcatg atgctgtgac agcggaatac
    23941 gctgaatgca tcgctcaaac cgtcgaggcg gctgtcagtc tcggtatcac ggctatcgat
    24001 caggaaacga tatgccgcta acaagcctgc cgggccgata aacttatcgg gattccacca
    24061 gaaagacgga caagaggttg aacaacatgc gcagagaata cattcataca gcccgtcgag
    24121 tttttcgcgc tgctctggca tctgtaaatg ctcgcgagct ggcggatttt gtccattatt
    24181 caacaggtaa ggcttaattt tctcatattg cgcatagaat tgtcccatgt ctaccaccaa
    24241 atcgcggatc accggtaaac ctggcagcgg gcgaatcaca atcttcttgc ccggctggtt
    24301 gagtgccgaa atcggggtaa tacaggccag accattcttg ccgttcatgt tcagaccgtc
    24361 ggaaccgcac acaccttcac ggcaggagcg gcggaacgac aggctgggat ctttctcttt
    24421 cagctggata agcgcatcca gcagcatcat gtcgcgacct tcttccgctt ccagggggta
    24481 atcctgcata cgcggagcat catcaacatc cgggttatag cgataaattg aaaactcgag
    24541 tctcattttc ctgtctccgc attagtaagt acgaatcttc ggcgggaatg ccgggcgcag
    24601 tttcggttcc atgttgacgc ttcggcgcgt catggattcc gactctggca gatacaggga
    24661 gtggcacagc cagttttcat catcacgatc cgggaagtcg aagcggctat gtgcgccacg
    24721 gctttcggta cggaagttgg cagaaacagc cgttgcatac gccgtttcca tcaggttatc
    24781 cagttccaag cactcaacgc gctgggtgtt gaactcgctg gaagtgtcat ccagacgggc
    24841 atttttcaga cgctcgcgga tcactttcaa ctgctcaagc cctttcgcca tcgcatcacc
    24901 ttcacggaag accgagaagt tatgctgcat acattcttgc agcgctttac gaatcgccac
    24961 cggatcttca ccgttacgat tattgttcca gcggtgcaga cgatccagag atgcttcaac
    25021 atcagactcg ctggcatcgc gcaatgcgcc ctgttcggcg atagactctt gcagatgcag
    25081 acctgccgca cgaccaaaga ccaccaggtc cagcagcgag ttgccgccca gacggttagc
    25141 gccgtgtacc gatacacaag cgatttcacc aacggcaaac agccccggaa caaccacatc
    25201 ttcgcctttc tcgttcaccg tcagcgcctg accggtcact ttggtcggaa taccgcccat
    25261 catgtagtga caggttggga taaccggaat cggctctttc accggatcga cgtgagcaaa
    25321 ggtacgggaa agctccagga tacccggcag acgggattcg agaacttctt tacccaggtg
    25381 atcgagtttc agtttcgcgt gtggccccca cggaccatca cagccgcgac cttcacggat
    25441 ttcaatcatg atggaacgcg ccaccacatc acggcccgcc aggtctttgg cgttcggcgc
    25501 ataacgctcc ataaagcgtt cgccatgttt gttcagcaga taaccgcctt caccacggca
    25561 accttcggtg accagtacgc ccgcaccggc aatgccggtc gggtggaatt gccacatttc
    25621 catatcctgc accggtacgc ctgcgcggat agccatgccg acaccgtcgc cagtgttaat
    25681 gtgggcgtta gtggtggact gataaatacg ccctgccccg ccagtcgcca gcacggtagc
    25741 gcgggctttg aaataaacca cttcaccggt ttcgatgcac agtgcggtac aacccaccac
    25801 cgcgccatcc tggtttttca ccagatccag cgcataccac tcggagaaaa tggtggtgtg
    25861 gtttttcagg ttctgctgat aaagcgtgtg caacagtgcg tgaccggtac ggtcagccgc
    25921 cgccgcagtg cgtgccgcct gctcgccgcc gaagtttttc gactgaccgc caaacggacg
    25981 ttgatagata cgaccatcat caagacgcga gaacggcagg cccatgtgtt cgagttccag
    26041 aatcgcttcc ggcccggttt tacacatata ttcaatcgcg tcctggtcac cgatatagtc
    26101 cgaccctttt acggtgtcgt acatatgcca ttcccagtta tcttcatggg tattacccag
    26161 cgcaacggta atgccgcctt gcgcagaaac ggtatgggaa cgggtcggga agactttaga
    26221 gagcagcgca caggtctggc cgctctggga aatttgcagc gccgcgcgca tacctgcgcc
    26281 accggcacca atcacaactg catcaaattc tctgactggc aatttcatca cacaccccac
    26341 accacaacga atccataaat cacgtaaacc accagtgcaa cgacaatcac cagttgcagc
    26401 atcaggcgca atgccagcgg tttaacgtag tcggtcaaca cctgccacat gccgatccag
    26461 gcatgaatca agatagaaaa cagcgccagc agggtgaaca ctttggtgaa cgcagaggcg
    26521 aagaaaccga tccagacttc atatgtcagt tcgccactgg tggcgaaaaa accgaccata
    26581 taaatgatgt agagcgtcag gacgatagcg gtagcgcgaa cgaggatgaa atcatgtacg
    26641 ccattgcgtc ctaatgcgga ggcgttgctt accatacgag gactcctgcg agaagtgaaa
    26701 gcacgacagt aataacaaag gagattttgg cggagcgttt acccgcttcg aatgtttctt
    26761 ccagatagcc aaaatccatc atcatgtggc gaatacctac gacgacgtga tacgccagag
    26821 cggtaaggat gccccacatg ataaatttga cgaagaagct gcccataatc gcggaagctt
    26881 gctcgaaacc ttcaggggaa gagaggctgg tacccagaag ccacagcagg atgcccactg
    26941 caacaaaggt gatcacaccg gaaacgcgat ggagaatgga cgctatcgcc gtgatgggga
    27001 accggatggt ctgtaggtcc agattaacag gtctttgttt tttcacattt cttatcatga
    27061 ataacgccca catgctgttc ttattattcc ctggggacta cgggcacaga ggttaacttt
    27121 ctgttacctg gagacgtcgg gatttccttc ctccggtctg cttgcgggtc agacagcgtc
    27181 ctttctataa ctgcgcgtca tgcaaaacac tgcttccaga tgcgaaaacg acacgttaca
    27241 acgctgggtg gctcgggatt gcagggtgtt ccggagacct ggcggcagta taggctgttc
    27301 acaaaatcat tacaattaac ctacatatag tttgtcgggt tttatcctga acagtgatcc
    27361 aggtcacgat aacaacattt atttaatttt taatcatcta atttgacaat cattcaacaa
    27421 agttgttaca aacattgcca ggaaaagcat ataatgcgta aaagttatga agtcggtatt
    27481 tcacctaaga ttaacttatg taacagtgtg gaagtattga ccaattcatt cgggacagtt
    27541 attagtggta gacaagttta ataattcgga ttgctaagta cttgattcgc catttattcg
    27601 tcatcaatgg atcctttacc tgcaagcgcc cagagctctg tacccaggtt ttcccctctt
    27661 tcacagagcg gcgagccaaa taacaaacgg gtaaagccag gttgatgtgc gaaggcaaat
    27721 ttaagttccg gcagtcttac gcaataaggc gctaaggaga ccttaaatgg ctgatacaaa
    27781 agcaaaactc accctcaacg gggacacagc tgttgaactg gatgtgctga aaggcacgct
    27841 gggtcaagat gttattgata tccgtactct cggttcaaaa ggtgtgttca cctttgaccc
    27901 aggcttcact tcaaccgcat cctgcgaatc taaaattact tttattgatg gtgatgaagg
    27961 tattttgctg caccgcggtt tcccgatcga tcagctggcg accgattcta actacctgga
    28021 agtttgttac atcctgctga atggtgaaaa accgactcag gaacagtatg acgaatttaa
    28081 aactacggtg acccgtcata ccatgatcca cgagcagatt acccgtctgt tccatgcttt
    28141 ccgtcgcgac tcgcatccaa tggcagtcat gtgtggtatt accggcgcgc tggcggcgtt
    28201 ctatcacgac tcgctggatg ttaacaatcc tcgtcaccgt gaaattgccg cgttccgcct
    28261 gctgtcgaaa atgccgacca tggccgcgat gtgttacaag tattccattg gtcagccatt
    28321 tgtttatccg cgcaacgatc tctcctatgc cggtaacttc ctgaatatga tgttctccac
    28381 gccgtgcgaa ccgtatgaag ttaatccgat tctggaacgt gctatggacc gtattctgat
    28441 cctgcacgct gaccatgaac agaacgcctc tacctccacc gtgcgtaccg ctggctcttc
    28501 gggtgcgaac ccgtttgcct gtatcgcagc aggtattgct tcactgtggg gacctgcgca
    28561 cggtggtgct aacgaagcgg cgctgaaaat gctggaagaa attagctccg ttaaacacat
    28621 tccggaattt gttcgtcgtg cgaaagataa aaatgattct ttccgcctga tgggcttcgg
    28681 tcaccgcgtg tacaaaaatt acgacccgcg cgccaccgta atgcgtgaaa cctgccatga
    28741 agttctgaaa gagctgggca ccaaagatga cctgctggaa gtggctatgg agctggaaaa
    28801 catcgcgctg aacgacccgt actttatcga gaagaaactg tacccgaacg tcgatttcta
    28861 ctctggtatc atcctgaaag cgatgggtat tccgtcttcc atgttcaccg tcattttcgc
    28921 aatggcacgt accgttggct ggatcgccca ctggagcgaa atgcacagtg acggtatgaa
    28981 gattgcccgt ccgcgtcagc tgtatacagg atatgaaaaa cgcgacttta aaagcgatat
    29041 caagcgttaa tggttgattg ctaagttgta aatattttaa cccgccgttc aaatggcggg
    29101 ttaattttta tatgcccaaa cacaaaaaat tgtaaaaata aaatccacta tcggacattc
    29161 atatattcta aaaaaaatat atcggtataa attataaaca aatcgatgat ttttaatcat
    29221 ctatcttcct ctcagttaca tgaataaacc accaaatata gcgcacccct ataatttcca
    29281 atactttatt catcaaacat ttaattcata ccatcaatta attactttta catttaataa
    29341 aattaataat atttttaaga aaaactaaaa acattcgaca atcaaataag atattcttgc
    29401 accgatatac ttttttcttt tctgttaaag gactcagtta tgtttaaagg acaaaaaaca
    29461 ttggccgcac tggccgtatc tctgctgttc acagcacctg tttatgcggc tgatgaagga
    29521 tccggtgaaa ttcactttaa aggtgaagtt attgaagcac cgtgtgaaat acatcaggat
    29581 gatattgata aagaggttga actcggtcag gtgaccacca gccacattaa tcagtcacat
    29641 cacagcgatg ccgttgctgt cgacctgcgc ttagtcaact gtgatctgga aaactccagc
    29701 aacggttccg gtggcaagat ttctaaagtt gcagtcacct ttgatagctc agcgaaaacc
    29761 accggcgcag atccgattct caacaacacc agcacgggtg aagccaccgg cgtcggcgta
    29821 cgtttaatga ataaagatca aagcaatatc gttttaggca ccgctactcc agatatcgac
    29881 ctggctccga cctccagcga acaaacgctg aatttctttg cctggatgga acaaattgat
    29941 caggctacac ctgtaacgcc aggcgcagtt acggcgaatg caacctacgt gctggattat
    30001 aaataagtat tactttggcg gcgctatcgc cgcctttcat aaaatattta tcaggtatgg
    30061 acaccgtgaa tatttatcga ctctcttttg tatcctgcct ggtcgtggcg atgccttgcg
    30121 cattggcggt cgaattcaac cttaatgttc tcgataaatc gatgcgcgac cgcattgata
    30181 tttcattatt aaaggaaaaa ggagtcattg ctcccggtga gtattttgtt agcgttgcgg
    30241 taaataataa ccaaatcagt aacgggcaaa agattaactg gcacaaaaat gacgataaaa
    30301 ccattccgtg catcaatgat ttactggtcg ataaatttgg cttaaaacct gaagtccgtc
    30361 agtcgttacc attgataaat cagtgcgtcg attttagctc ccgacctgaa atgctcttca
    30421 atttcgatca agccaatcag caactgaata ttagtattcc gcaagcctgg ctggcgtggc
    30481 actcagaaaa ctggacccca ccctccacat ggaaagaagg tgtcgccggt atcctgatgg
    30541 attacaactt gtttgccagc agctaccgcc cacaggacgg cagcagcagc actaacctga
    30601 acgcctacgg taccaccgga attaacgccg gggcatggcg cttacgtagt gattatcagt
    30661 tgaatcagac tgatagcgat gataaccatg aacagtcagg cggaatatcg cgcacctatc
    30721 tttttcgtcc attaccgcaa ttaggctcta aattaaccct cggcgaaacg gattttagtt
    30781 ccaatatttt cgacggtttt tcttataccg gcgcggcact ggcaagtgac gagcgaatgt
    30841 tgccatggga actacgcggc tacgccccac aaattagcgg tattgcacag accaatgcca
    30901 cggtgacgat cagtcaatca ggccgcgtca tttaccagaa aaaagtccca ccaggcccat
    30961 ttatcattga cgaccttaat cagtctgttc agggcacact ggatgtcaaa gtgacggaag
    31021 aagatggtcg ggtgaacaat ttccaggttt cggcagcatc gacgcccttc ctgactcgtc
    31081 agggacaggt tcgctataaa ctggccgcgg gtcagccacg gccctccatg tcacatcaaa
    31141 ctgaaaatga aacctttttt agcaatgaag tttcctgggg gatgctgtca aacacctcgc
    31201 tgtacggcgg cctgctgctt tctggtgatg actaccattc tgccgcaatg ggtattgggc
    31261 aaaatatgct gtggcttggt gcgctgtcgt ttgatgtcac gtgggccagt agccattttg
    31321 atactcagca ggacgagcgg ggcttaagct accgttttaa ttacagcaaa caagtggatg
    31381 ccactaacag cacgatttcc ctcgccgctt atcgtttctc cgatcgtcat tttcacagct
    31441 acgccaacta tctggatcac aaatacaacg acagcgatgc gcaggacgaa aaacagacga
    31501 tcagcttatt gtgggccaac cgattacccc actaaacctc aatctttacg ccaacctgct
    31561 acatcaaacc tggtggaatg cagacgcctc cacgaccgcc aacatcacgg caggttttaa
    31621 tgttgatatt ggtgactgga gagatatctc tatttcgacg tcattcaata caacccatta
    31681 cgaagataaa gatcacgaca accagattta cctgtcgatt tcgctcccct tcggtaacgg
    31741 tggtcgggtt ggctatgaca tgcaaaacag tagccacagc accacacacc gcatgtcgtg
    31801 gaacgatacg ctggatgaac gtaatagctg gggcatgtct gccggactgc aatccgaccg
    31861 tcctgacaat ggagcccagg tgagcggtaa ctatcagcac ctgagttcag cgggtgagtg
    31921 ggatatttct ggtacctatg ccgccaatga ttacagttcc gtcagcagca gctggagcgg
    31981 ttctttcacc gcaacccaat atggtgcagc gtttcatcgc cgcagctcca ccaatgaacc
    32041 acgcctgatg gtcagcaccg atggcgtggc agatattccg gttcagggca atctcgacta
    32101 caccaaccat tttggcattg cggtggtgcc gttgatttcc agttaccagc cttccaccgt
    32161 ggcggtgaac atgaatgact tacccgacgg cgtaacagtt gcagaaaacg ttatcaaaga
    32221 aacgtggatt gaaggcgcga taggttacaa atcactggct tcccgttccg gtaaagacgt
    32281 taacgtcatc attcgcaacg ccagcggtca gtttcctccc ctcggagcgg atatccgcca
    32341 ggatgacagc ggcattagcg tggggatggt tggcgaggaa ggacatgcct ggttaagcgg
    32401 agtcgctgaa aatcaaaagt ttaccgtggt ctggggtgat agccagcatt gctcgctcca
    32461 tcttcctgaa catatggaag acaccgcaaa tcgcctgatt ttaccttgtc attaattaag
    32521 gaaatgaaaa tgacatttat gaaaggacta ccccttttgt tgttggttgc cagtctgtgc
    32581 agccacgctg cactacaacc cgatcgcact cgtattgtgt ttaacgctaa tgataaagct
    32641 accagtctgc gggtggataa tcgtagcgat aaactcccct acctcgctta ttcctggctc
    32701 gaaaatgaaa aaggggagaa aagcgatgat cttctggtcg ccctgccgcc gatacaacgt
    32761 ctggaaccga aagcaacaac gcaggtacgg atagtaaagc aagcctcgac cacaaaatta
    32821 ccgggcgatc gcgaaacgct atttttctac aatatgcgtg aaattcctcc cgccccggaa
    32881 aaaaacagcg accatgccgt tctgcaagtc gccattcaga gtcgtatcaa ggtgttctgg
    32941 cgaccggctg cattacgcaa gaaagcagga gaaaaggtcg aactgcagtt acaggtcagc
    33001 cagcagggca accaactgac actgaaaaat cctaccgcgt attatctgac cattgcctac
    33061 ctgggacgaa atgaaaaagg cgttctcccc ggcttcaaaa ccgtgatggt tgcgcctttt
    33121 agtacggtca acacaaacac cggaaattat agcggcagcc agttttatct cggttatatg
    33181 gatgattacg gcgcgttacg catgacgacg ctcaactgca gcggacaatg ccatttacaa
    33241 gccgtggagg cgaagaaatg agtgctggca agggagtgtt gctcgtcatc tgtctgttat
    33301 ttctgccgtt gaagtctgcg cttgccctga actgctactt tggttcatcc ggtggctcgg
    33361 tagaaaaatc agaagcgatt caaccttttg ctgtaccagg caatgccaaa ctcggcgata
    33421 agatttggga atctgacgat attaaaattc ccgtctattg tgacaacaat accaacggca
    33481 attttgaaag tgagcatgtt tatgcctggg taaaccccta cccgggggtg caggatcgct
    33541 attatcaact cggcgtaact tacaatggcg ttgattatga cgccaaccag ggaaaaagcc
    33601 gcatcgacac taaccagtgt attgacagta agaacattga catttatacc cctgagcaga
    33661 ttatcgcgat gggatggcag aacaaaattt gctcgggtga tcccgctaat attcacatgt
    33721 cgcgtacctt tctcgctcgc atgcggttat acgtcaaaat acgagaaatg ccgccgcatg
    33781 attatcaaag tacgcttagc gactatatcg tcgtgcaatt tgacggtgcc ggtagcgtta
    33841 acgaagaccc tactgcccaa aacctgaaat atcatattac tggtctggaa aacattcgcg
    33901 tgctggattg cagcgtcaat ttttccattt caccggaaac acaggtgatt gattttggta
    33961 aatttaattt gctggatatt cgtcgccaca caatgtcgaa aacattcagt attaagacga
    34021 caaaatcaca aaatgaccaa tgcaccgacg gatttaaagt cagttcctcc ttttataccg
    34081 aagaaacgtt ggttgaagaa gataaagcat tgttgattgg taatggtctg aagctacgtt
    34141 tgctggatga aaacgcctcg ccttatacct tcaataaata tgctgaatac gccgatttca
    34201 ccagcgacat gttggtctac gaaaaaacct atacagctga actttcgtcc attgcaggta
    34261 cccccatcga ggctgggccc ttcgatacag tggtgctttt taaaattaac tataactgat
    34321 ttgccatgat gccctttgca gccggaaagg gcatctcttt cagaaaattg ctgattttcc
    34381 ccttttcctt gattgctgtt attttttcct ggcccggctt tctttatgct gtatcgatac
    34441 accaaagaga aaataatgag ggagcgaagg atgccagttt tgcagtgggg aatgttatgt
    34501 gtgctatcac ttctcctttc tattggtttc ctcgcggtac atctcccggc ggcgctatta
    34561 cttgggccga tgatcgccgg gatcatcttc agtatgcgcg gaattactct gcaacttccc
    34621 cgctccgctt ttctcgccgc gcaggccatt cttggctgca tgattgcgca aaacctcact
    34681 ggttcgattc tcaccacgct ggccgccaac tggccgatcg tgcttgctat tttactggtg
    34741 acgctgcttt ccagcgccat cgtgggttgg ttattggtgc gctatagctc actgcctgga
    34801 aataccggtg cctggggttc ttctccaggc ggcgcagcgg caatggtcgc tatggcgcaa
    34861 gattacggcg cagatattcg cctggttgca tttatgcaat atctgcgagt gctgttcgtc
    34921 gcgggggccg ctgttctggt cacccgcatg atgctgggtg ataacgctga agcggttaac
    34981 cagcaaatcg tctggttccc gccagtgagc attaatctcc tgcttacgat tttgctcgca
    35041 gtcgttgccg gtacggcggg atgcatgctg cgtcttcctt ccggcacgat gctcatcccg
    35101 atgctggcgg gcgcagtgct ccagtctggt cagctcatca ccatcgaact accggaatgg
    35161 ctgctggcga tggcgtatat ggcaattggc tggcggattg gtcttggttt cgataaacaa
    35221 atcttactgc gggcactacg cccgctaccg caaatcctgc tgtcgatttt tgctctgctg
    35281 gctatttgtg cgggtatggc gtgggggctg acccggttta tgcacattga ttttatgact
    35341 gcctacctcg ccaccagtcc cggcgggctt gataccgtgg cggtcatcgc cgcagggagc
    35401 aatgccgata tggcgcttat tatggcgatg caaaccctgc gcctgttcag tattttgctg
    35461 acggggccag ccattgcgcg gtttatttca acctatgcgc cgaagcggtc ggcctagtgc
    35521 tggcagccag ggcaccagta aaacgggcga gatgacagcg tggttttctc aatgatgctg
    35581 ccacagcgtt cgcacggttc gccatcacga tgaaaaacct taaagcgaaa cagcgcccca
    35641 tgatgcttat tctcatccac cagccccctc gtagcgtagg aaaatcgagg aatatccagt
    35701 aacgcgtgtg cgagtgcatc cagttgcgcc gcattgagat ctttcgcttt atgatttcca
    35761 gtcaacccaa cctgccagag gatctccacc cgcaaataat tgccaagccc agccagaaac
    35821 gcctgatcga gcagtaatcc tgcaaactga cggttacgaa agcgcggtga caataaccgt
    35881 tcctttacca cctccggcgt cagattcgga tccagcacat cgggaccaac gcgttgtaaa
    35941 aacggatgcg tggtcagttg ttccggcctc aacatctcaa tatccgaggc gctataaagc
    36001 agaatggttt tgtcagccgt ttgcagtttt acccgcaata ctcgcgtggt ctgcggctct
    36061 tcgccggtat caaccacgcg ccagacgccg taaagctgat tatggctgta gagcgttaag
    36121 tcgttggaaa aatgagttaa caacgcctta ccacgcgttt ccacatgggt aacgtgttga
    36181 ccgataagtt gtgattgata agtttttaac tgcgggaagg caaaccagac atcagttagt
    36241 ggtttgcctt tgatcgccgc ctccaggtta tccgctgcac ggcggatctc cgggccttca
    36301 ggcatggtga tatccttttt tcattcttta ttaatcgggg ttatgctgcg acaacaatct
    36361 ccttttcggc aaatgtagag cgtagtcggc gggcgaacgc cagtgcgtgc tcgccgtcgc
    36421 catgcaggca gactgtttgc gccgtgaccg ttgcccattc gccggtgata cttttgactc
    36481 tgccgtgttg caccatctct agcgtttgcg ccaacgcctg ttcttcgttt tcaatcaacg
    36541 cgcctcgctg acttcgcggc accagcgagc cgtcagcctg ataaccgcga tcggcaaaca
    36601 cttcctcgcg cgttgtcagg ccatattgct tgcctgcacg aatcagctcg cttcctgcca
    36661 gcccaacgag aatcaatgcc ggatcgcaag cgtataccgc tctggcgatg gcgtctgcca
    36721 gttgtgcttc tttcgccgcc tggttgtaca acatgccgtg cggtttgaca tgacgcatta
    36781 cgccgccttg cgcacgggca atcgttgcca gcgcgccaat ttgatacagc gtctgggcgt
    36841 aaacggtttc tggcggcagc tgcatggcgc tgcgaccaaa attttccctg tcgggaaaac
    36901 tcgggtgagc gccaatcgcg acaccatttt ttattgcttc acgcacgcaa gcctgcatga
    36961 tttgcgcatc gcctgcatga aatccacagg caatattggc agaggaaacc agcgttaata
    37021 gctctgcgtc gctggcgcag ccttcgccca gatcggcgtt caggtcaatt ttcattgtgc
    37081 agccgccaca ctaattgttc gaaataacgt tgctgatctt gccgcgcttt cagtgcctct
    37141 tccagtgaac actggacaaa atgaatcggc tgaccgagcg gaatttgcgc cagatggtac
    37201 atatcagcct caatgataca ggcaatacgc gggtaaccat cggtggtctg tgcgtcgttc
    37261 atcaacacaa tcggctgccc gttatgtggc acctgcacca cgcccggtaa caaaccgtga
    37321 gataacagtt cgcgatcggt ggtgcgtttt aaaatttgcc cctgtaagcg atagcccatg
    37381 cggttacttt gcgagctaag ctgccagggc gaacgccaga atgcatcctg cgaggcgcga
    37441 tcgaactcat gatattccgg ccccggcaag gcgcgaatgc ggttgcccca cagcagctgt
    37501 ttaacgccct gcgcttccat aaaatcacgc ttcgatttgc caatcgggag tcggtcacca
    37561 tccttcagta aacggccttc cagcccgcca atccccactt tgagatcggt gctgcatgac
    37621 cccattaccg gcggaacatc aataccaccc gcgaccgcca gataactgcg catcccgtgc
    37681 tgcgggcgtt taagcgttaa acgctggcct gctttcatcg gcaatcgcca gccggtccag
    37741 acggcattat catccagccg cgcttcgcaa ccggcacccg tcagagcaaa ccacccatca
    37801 gtttcgaact caaccgttaa ctgaccgagc gtgatctcca gcgcgggggc attggcgtca
    37861 ttacccacca gcaggttagc aatgcgtaac gcaggcatat ccagtgcgcc gcagtggctg
    37921 attcccgact ggcgaaaacc gtgacgaccg ccatcctgca cagtggtata catgcccgca
    37981 cgaataatct tcagcatact ccctccttct gcggtacaaa gcgcacgctg tctcccggac
    38041 gtaataagat gggttcgtca cgcgccggat caaacaggct gagtgaggta tgaccaatca
    38101 actgccagcc acccggcgtt gccagcggat aaacaccagt ctgcggcccg ccgatcccga
    38161 cagaacctgc cggaacgagc aagcgcggtt cagcgcgccg tggcgtgtgt agttgttccg
    38221 gcaaactccc gagatacggg aagcccggtt gaaaacctaa aaaccagacc acgtattcca
    38281 cggaggagtg caattcaaca acctgttttt cgctaaaccc gcaatgcgcc gcgaccaccg
    38341 ccaaatccgg tccgcctgca ccaccgtaaa ccaccggaat ttcaataaag cgagactcag
    38401 gttccagggc ctcgctctcc tcccaccagc gttgcaaacg ctctatggca tccagcgcca
    38461 gcgactcagg attacgcaaa atcaccgtga tattgttcat gccgggaatg gcttcaacca
    38521 cattcggcat atccaccaga cgctgcgcca gtcgccagat ccgtttctgg ctagccagcg
    38581 tcaccggcgg ttccagttcc agcactaccg ccgtttcacc tatcagataa caacgcgctc
    38641 gttgcacttt cgatccctct gattattcgt tatgcaggat taggaatatc aataaaggtc
    38701 acatcaagat cggtattttc attcagccac tcgctcaatg cgcgaatacc accacgttca
    38761 gtggcatggt gacctgcagc ataaaaatgc aacccctgct cgcgggctga atgaatggtc
    38821 tgttcagaaa cttcgccagt aataaaagca tccacgccaa aacgcgcggc gctatcgata
    38881 aaactttgcc cgccgcccgt gcaccaggcg acgcgctgga ccacctcagg tccggtatcg
    38941 ccacaccata acggcttacg tcccagacgc gcttcaatcc aggaggctag ttccagtccc
    39001 ggcacaggca tggtcagctc tccccacggc accaacggct caatttcgcc catgaccgtg
    39061 atccccagta acgccgccag ctgtgcgtta ttgcccagct caggatgtac gtcaagcggc
    39121 aaatgccagc catacaggtt gatatcattc gccagcaacg tttttaaacg gttacgcttc
    39181 attccgcgaa tgaccggaga ctctcctttc cagaagtagc catgatgcac aatgaccgca
    39241 tccgcgccca gacgcactgc ctcatcgagc aaagcctggc tggcggtgac accggtaaca
    39301 attttttgca ccgtctcttt gccttctacc tgcaaaccat tcggcgcgta atcactaatc
    39361 gccgcgctgt tcagtttttc gttaatcagt tgttccagtt cggtgttttt catcttcgcg
    39421 ccttccgtat tctgatagct acaccgttaa catagcgtta cctcgcctgt tcgtcgatag
    39481 ccaatctttt gacatattaa gaaacaaacc cgacgccaaa agacgcaaat tgtttatatc
    39541 atcacgcttc agctaatgta tatttatgca atcagacagc atattgcata aataatcaga
    39601 tctgtatcaa agttttaact ttaaggaaac acaagcacgc cgcgcaacta ttacgtgacg
    39661 ttgcagtcaa ttgtggaatt attatgaata aacacgcatc acaaccgcgc actatttact
    39721 atgtcgttgc gctgcaaatc tgggaatatt ttagctttta cggcatgcgt gccctgctga
    39781 ttctctatct caccaatcaa ctaaaataca acgatactca cgcctacgag ttatttagcg
    39841 cctactgttc gctggtgtat gtcacgccaa tcctcggtgg ctttttggcg gataaagttc
    39901 tcggcaatcg catggcggtg atgctggggg cgttgttgat ggcgatcggt catgtggtgc
    39961 tgggtgccag tgagatccat ccgtcattcc tctatctgtc cctggcgatt atcgtttgtg
    40021 gctatggtct gtttaaatcc aatgtcagtt gcctgctcgg cgagctgtat gagccaaccg
    40081 atccgcgtcg tgatggcggt ttctcgctga tgtatgcagc gggtaacgtg gggtctatta
    40141 tcgcacctat cgcctgtggt tacgcccagg aagagtacag ctgggcgatg ggctttggcc
    40201 tggcggcggt gggtatgatc gcgggtctgg tcattttctt atgtggcaat cgtcatttca
    40261 cacatacccg cggcgttaat aaaaaagtac tgcgtgcaac aaactttctc ctgcctaact
    40321 ggggatggct gctggttctg ctggtggcaa cgcctgcgct gattaccgta ctgttctgga
    40381 aagagtggtc ggtatacgcc ttaattgtcg cgactattat tggcctgggt gtactggcaa
    40441 aaatttatcg caaagcagaa aaccagaaac agcgtaagga gctggggctg attgtgacgc
    40501 tcaccttctt cagtatgttg ttctgggcct tcgcacaaca gggcggcagc tcaattagcc
    40561 tttatatcga ccgcttcgtt aaccgcgata tgtttggtta taccgttccg actgctatgt
    40621 tccagtcgat taatgccttc gcagttatgc tatgcggtgt gttcctggcg tgggtggtta
    40681 aagaaagcgt cgcaggtaat cgtaccgtgc gcatctgggg aaaatttgct cttggccttg
    40741 gcctgatgag cgccggattc tgcattctga ccttaagcgc ccgctggtcc gcaatgtatg
    40801 gtcactcttc tctgccactg atggtgttag gcctcgcggt gatgggcttt gcggaactgt
    40861 ttatcgaccc ggttgccatg tcgcaaatta cgcgcattga aattccgggc gtgaccggcg
    40921 tattaaccgg catctacatg ctgctttctg gcgcgatcgc gaactatctg gcgggcgtga
    40981 ttgccgatca gacatcgcag gcttcgtttg atgcttccgg ggcgatcaac tactccatca
    41041 atgcatatat tgaagtattt gatcaaatta cctggggcgc actggcgtgt gtaggactgg
    41101 tactgatgat ttggctgtat caggcgctga aattcagaaa ccgcgcgctg gcgctggagt
    41161 cataataggc cagcaccgat gcctgatgcg acgcttgacg cgtcttatca ggccggtgat
    41221 gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt
    41281 ttctatcagc tgtccctcct gttcagctac tgacgggggg gt
//
LOCUS       DAETQN010000019        41142 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.019, whole genome shotgun sequence.
ACCESSION   DAETQN010000019 DAETQN010000000
VERSION     DAETQN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.019"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            32..943
                     /locus_tag="K0W68_001100"
     CDS             32..943
                     /locus_tag="K0W68_001100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HBI3072729.1"
                     /translation="MKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLIT
                     RITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGNLLNEENPHQLQQLIRLPG
                     QQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYTYPLSPVNSMDPLGLYEFK
                     SKNIDDIGIFALAMCNGESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKC
                     PEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYL
                     GTPNNTYLKYNPKAKGNGVTIIRQGSN"
     gene            945..1181
                     /locus_tag="K0W68_001101"
     CDS             945..1181
                     /locus_tag="K0W68_001101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072730.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(1354..2029)
                     /locus_tag="K0W68_001102"
                     /pseudo
     CDS             complement(1354..2029)
                     /locus_tag="K0W68_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(2174..2782)
                     /locus_tag="K0W68_001103"
     CDS             complement(2174..2782)
                     /locus_tag="K0W68_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBI3072731.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(2966..3283)
                     /gene="metJ"
                     /locus_tag="K0W68_001104"
     CDS             complement(2966..3283)
                     /gene="metJ"
                     /locus_tag="K0W68_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBI3072732.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            3560..4720
                     /gene="metB"
                     /locus_tag="K0W68_001105"
     CDS             3560..4720
                     /gene="metB"
                     /locus_tag="K0W68_001105"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBI3072733.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4723..7155
                     /gene="metL"
                     /locus_tag="K0W68_001106"
     CDS             4723..7155
                     /gene="metL"
                     /locus_tag="K0W68_001106"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBI3072734.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            7504..8394
                     /gene="metF"
                     /locus_tag="K0W68_001107"
     CDS             7504..8394
                     /gene="metF"
                     /locus_tag="K0W68_001107"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBI3072735.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            8723..10903
                     /gene="katG"
                     /locus_tag="K0W68_001108"
     CDS             8723..10903
                     /gene="katG"
                     /locus_tag="K0W68_001108"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBI3072736.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            10997..11902
                     /gene="yijE"
                     /locus_tag="K0W68_001109"
     CDS             10997..11902
                     /gene="yijE"
                     /locus_tag="K0W68_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBI3072737.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(11929..12546)
                     /locus_tag="K0W68_001110"
     CDS             complement(11929..12546)
                     /locus_tag="K0W68_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBI3072738.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(12820..13923)
                     /gene="gldA"
                     /locus_tag="K0W68_001111"
     CDS             complement(12820..13923)
                     /gene="gldA"
                     /locus_tag="K0W68_001111"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HBI3072739.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(13934..14596)
                     /gene="fsa"
                     /locus_tag="K0W68_001112"
     CDS             complement(13934..14596)
                     /gene="fsa"
                     /locus_tag="K0W68_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBI3072740.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(14608..17109)
                     /gene="ptsP"
                     /locus_tag="K0W68_001113"
     CDS             complement(14608..17109)
                     /gene="ptsP"
                     /locus_tag="K0W68_001113"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBI3072741.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            17418..18497
                     /locus_tag="K0W68_001114"
     CDS             17418..18497
                     /locus_tag="K0W68_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBI3072742.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTGGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            18512..18832
                     /locus_tag="K0W68_001115"
                     /pseudo
     CDS             18512..18832
                     /locus_tag="K0W68_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            18883..21179
                     /locus_tag="K0W68_001116"
                     /pseudo
     CDS             18883..21179
                     /locus_tag="K0W68_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            21145..22023
                     /locus_tag="K0W68_001117"
     CDS             21145..22023
                     /locus_tag="K0W68_001117"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBI3072743.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            22025..22366
                     /locus_tag="K0W68_001118"
     CDS             22025..22366
                     /locus_tag="K0W68_001118"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBI3072744.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(22353..23204)
                     /locus_tag="K0W68_001119"
     CDS             complement(22353..23204)
                     /locus_tag="K0W68_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3072745.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(23419..25152)
                     /locus_tag="K0W68_001120"
     CDS             complement(23419..25152)
                     /locus_tag="K0W68_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBI3072746.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(25335..27986)
                     /gene="ppc"
                     /locus_tag="K0W68_001121"
     CDS             complement(25335..27986)
                     /gene="ppc"
                     /locus_tag="K0W68_001121"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBI3072747.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(28338..29489)
                     /gene="argE"
                     /locus_tag="K0W68_001122"
     CDS             complement(28338..29489)
                     /gene="argE"
                     /locus_tag="K0W68_001122"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBI3072748.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            29643..30647
                     /gene="argC"
                     /locus_tag="K0W68_001123"
     CDS             29643..30647
                     /gene="argC"
                     /locus_tag="K0W68_001123"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBI3072749.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            30655..31431
                     /gene="argB"
                     /locus_tag="K0W68_001124"
     CDS             30655..31431
                     /gene="argB"
                     /locus_tag="K0W68_001124"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBI3072750.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            31492..32865
                     /gene="argH"
                     /locus_tag="K0W68_001125"
     CDS             31492..32865
                     /gene="argH"
                     /locus_tag="K0W68_001125"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBI3072751.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            33132..34049
                     /gene="oxyR"
                     /locus_tag="K0W68_001126"
     CDS             33132..34049
                     /gene="oxyR"
                     /locus_tag="K0W68_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBI3072752.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPIADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(34032..35432)
                     /gene="sthA"
                     /locus_tag="K0W68_001127"
     CDS             complement(34032..35432)
                     /gene="sthA"
                     /locus_tag="K0W68_001127"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBI3072753.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            35753..36403
                     /gene="fabR"
                     /locus_tag="K0W68_001128"
     CDS             35753..36403
                     /gene="fabR"
                     /locus_tag="K0W68_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBI3072754.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            36403..36762
                     /locus_tag="K0W68_001129"
     CDS             36403..36762
                     /locus_tag="K0W68_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBI3072755.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(36802..37902)
                     /gene="trmA"
                     /locus_tag="K0W68_001130"
     CDS             complement(36802..37902)
                     /gene="trmA"
                     /locus_tag="K0W68_001130"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBI3072756.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      38016..38206
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            38271..40115
                     /gene="btuB"
                     /locus_tag="K0W68_001131"
     CDS             38271..40115
                     /gene="btuB"
                     /locus_tag="K0W68_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBI3072757.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            40060..40917
                     /gene="murI"
                     /locus_tag="K0W68_001132"
     CDS             40060..40917
                     /gene="murI"
                     /locus_tag="K0W68_001132"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBI3072758.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9515 a        10252 c        11319 g        10056 t
ORIGIN      
        1 cacgtagggt gaagccgggc tgaagcgggt ggtgaaaaag gaacacgcgg acggcagcgt
       61 cacgcagagt cagtttgacg ccgtgggcag gctcaggaca cagacggatg ccgcaggccg
      121 gacaacagaa tacagcccgg atgtggtgac gggtctcatc acgcgcatca ccacgccgga
      181 tggcagggca tcggcgtttt actataacca ccacagccag ttaacgtcag ccaccgggcc
      241 tgacgggctg gaaatacgcc gggaatatga tgaatggggc aacctgctga atgaagagaa
      301 cccgcatcag ctgcagcagc ttattcgttt gccggggcag cagtatgatg aggagtccgg
      361 cctgtattat aaccgccacc gctattatga cccgctgcag gggcggtata tcactcagga
      421 tccgattggg ctgaaggggg gatggaacct gtatacatat ccgctgagcc cggtgaatag
      481 catggatcca ttaggattat atgaatttaa atcaaaaaat attgatgata ttggaatatt
      541 tgcattggca atgtgtaatg gagaatcaat taacgagaat aaagaatatg gtggactaat
      601 atgtaagaag caaggtgaat atttccccat gaatccgata agttcaaatg ataatgatag
      661 tgtagacttg cgaaatataa aatgccctga aggttcagag agagtaggcg attatcacac
      721 tcacggtttt tactctgacg ataaaggaaa taaagtaaca aaagaaaatg atgtttatga
      781 tagtctaaat ttttcaagca aagatttaac gaattcttat atgaatggaa tgggaaaaaa
      841 agaatacagt agttacttgg gaacaccaaa taacacctat ctaaaatata atcccaaagc
      901 taaagggaat ggagttacaa ttatcaggca agggagtaat taaaatgaag gcaatacaat
      961 gttgttattt ccttgcttta gtctccagtc atttaatggc tggagttatg ggtgatgata
     1021 atatattttt aagtaattcg gattcagctg ctttatattg tgtggctaaa atatttccag
     1081 atggagaaaa tattttaata tataaagtaa atataattaa tgctagttca aaaaaagatg
     1141 ttttttttat gaaagcccaa catcaaccca caatgacata atggggggcg ggggaatgtc
     1201 atatgcctat gaatatgatg taaaatataa tatatgtcat ttgagaaaaa agagcagaat
     1261 gaggtaaaaa tctttggctg tgtattcagt ctggtaatca tccaaggaaa gcttatcaga
     1321 agggtgatga ctccaattag tagaacatat attttcgata aaagcaccga tgactttttc
     1381 atgaagttct acagaacgcg agaaacagat ggttctgcgg gccagacgtt tgattcgggt
     1441 tctcagggtc agattgttac gctcaatgtg ctgagtaaaa atcttaccag tcagatgctt
     1501 ctccttcggt aactctctag cgtaactacc ccattcgtca gtggtcaaca tgccgatggt
     1561 aaaagggtaa gcagcgcccg caattcacga caggtttcgt cagtccgggg gccaaaagtg
     1621 taggccagaa caccaccagt tttcgtgttg tacacgtacc agagccagtg ctgtcgcgct
     1681 ttactgccga caaagctcca ttgctgacta agtttgcaga tgagtgccac atcagcatga
     1741 gcgaccgggg atgacgttat tcttcgtggc gagagttttt taaagttcgg atgactgtat
     1801 taatgccgat tttcagcgta cgggcagtat cgcgaacccc ggcaccgttg aaagtcattt
     1861 cagcgatttg gtctttaact cccggtttac ggggctcgta acagtaactg agctgaaata
     1921 cccggtgaca gtcacggccg cggaatcgct catgtctttt tgggttctgc ccgtgacggt
     1981 aaacctgaac ggactggcaa cgggggcaat gaaggttaac tgtgaccatg aaacaacctt
     2041 aaaagcgagt attatatcct taattcaact aattggaggc atcaccgttt accggagcga
     2101 cagcttgcgg aaaggtcgtg caactacaaa gcaacaaaca gactacggcg ggaattattt
     2161 ttttcatcat ccattaccac gaaaacagcg gccactcttt ggcgactgtg gtgagttgtg
     2221 gcgcgtcgaa aacggcttgt ggtgaaacta ccgtttcctc aagcggaata tttttgccgt
     2281 agcgttcttt gagcttagcc tggaccagcg ggttgctgag atcgaactct ttcaacttct
     2341 gctgctcgcc cacgcgcatt cccagcgcgt tctggtaaac cttccacacc acttccgaac
     2401 agtactggcg atcgtcgctc catgagaagc tgaaatcata cggtttaccg agataacgtt
     2461 ttgccgtttg cgccagtttt tgctgttgtt caacactcag tccgccttca acgcggcgaa
     2521 caacgtattt gcccttttca ccatgggcga tccactgctt gagcgggggg tatttcaccg
     2581 ggccgaccgc ttcaaaaacg tagggctttt tgttgcgtat caccagcata ccggtgtggc
     2641 tataatcgga atgggtcgcc agttggatcg ctttactttg cgatgagcga gagatctgaa
     2701 agatgatgtc gccggtttgt ggctgccagg caaaggcagg tacagaaacc agcaggctga
     2761 ggatcagcag cctgtttttc atagttaaac gtccatgtat aaaaagcggt gggtcgcaga
     2821 caacgtgctc gttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc tacaagttcg
     2881 tgcaaattca ataaattgca atatgacgta ggcctgataa gcgtagcgca tcaggcgatt
     2941 ccactccgcg ccgctctttt ttgctttagt attcccacgt ctccgggtta atccccatct
     3001 cacgcatgat ctcttttgcc gcttccggga tttcgtcgct gcgctcttta cgcagatcgg
     3061 catcatccgg caaaggttgc ccggtaaagg catgcagaaa cgcttcgcac agcagctcgc
     3121 tgttggtagc gtgacgcagg ttgttcacct gacgacgcgt gcgttcatcg gtgaggattt
     3181 ttaacacctt aagaggaatg gaaaccgtaa tctttttgac ttgttcactc ttcttgccgt
     3241 gctcagcgta tgggctgata tattcgccgc tccattcagc catgagatac ttaatcctct
     3301 tcgtcaataa attgagacca gaccacacaa tttatgtggg tactgaccgt aaacccgcat
     3361 agtttaccgt acaggcgtta ccgtgacatc gtgtaatgca cctgtcggcg tgataatgca
     3421 tataatttta acggctattt gggatttgct caatctatac gcaaagaagt ttagatgtcc
     3481 agatgtattg acgtccatta acacaatgtt tactctggtg cctgacattt caccgacaaa
     3541 gcccaggaaa cttcatcaca tgacgcgtaa acaggccacc atcgcagtgc gtagcgggtt
     3601 aaatgacgac gaacagtatg gttgcgttgt ccctcccatc catctttcca gcacctataa
     3661 ctttaccgga tttaatgaac cgcgcgcgca tgattactcg cgtcgcggca acccaacgcg
     3721 cgatgtggtt cagcgtgcgc tggcagaact ggaaggcggt gctggcgcag tacttactaa
     3781 taccggcatg tccgcgattc acctggtaac gaccgtcttt ttgaaacctg gcgatctgct
     3841 ggttgcgccg cacgactgct acggcggtag ctatcgcctg ttcgacagtc tggcgaaacg
     3901 cggttgctat cgcgtgttgt ttgttgatca aggcgatgaa caggcattac gggcagcgct
     3961 ggcagaaaaa cccaaactgg tactggtaga aagcccaagt aatccattgt tacgcgtcgt
     4021 ggatattgcg aaaatctgcc atctggcaag ggaagtcggg gcggtgagcg tggtggataa
     4081 caccttctta agcccggcat tacaaaatcc gctggcatta ggtgccgatc tggtgttgca
     4141 ttcatgcacg aaatatctga acggtcactc tgacgtggtg gctggcgtgg tgattgctaa
     4201 agacccggac gttgtcactg aacttgcctg gtgggcaaac aatattggcg tgacgggcgg
     4261 cgcgtttgac agctatctgc tgctacgcgg gttgcgaacg ctggtgccgc gtatggagct
     4321 ggcgcagcgc aacgcgcagg cgattgtgaa atatctgcaa acccagccgt tggtgaaaaa
     4381 actgtatcac ccgtcgctgc cggaaaatca ggggcatgaa attgccgcgc gtcagcaaaa
     4441 aggctttggc gcaatgttga gttttgaact ggatggcgat gagcagacgc tgcgtcgttt
     4501 cctgagcggg ctgtcgttgt ttacgctggc ggaatcatta gggggagtgg aaagtttaat
     4561 ctctcacgcc gcaaccatga cacatgcagg catggcacca gaagcgcgtg ctgccgccgg
     4621 gatctccgag acgctgctgc gtatctccac cggtattgaa gatggcgaag atttaattgc
     4681 cgacctggaa aatggcttcc gggctgcaaa caaggggtaa aaatgagtgt gattgcgcag
     4741 gcaggggcga aaggtcgtca gctgcataaa tttggtggca gtagtctggc tgatgtgaag
     4801 tgttatttgc gtgtcgcggg cattatggcg gagtactctc agcctgacga tatgatggtg
     4861 gtttccgccg ccggtagcac cactaaccag ttgattaact ggttgaaact aagccagacc
     4921 gatcgtctct ctgcgcatca ggttcaacaa acgctgcgtc gctatcagtg cgatctgatt
     4981 agcggtctgc tacccgctga agaagccgat agcctcatta gcgcttttgt cagcgatctg
     5041 gaacgcctgg cggcgctgct cgacagcggt attaacgacg cagtgtatgc ggaagtggtg
     5101 ggccacgggg aagtatggtc ggcacgcctg atgtctgcgg tacttaatca acaagggctg
     5161 ccagcggcct ggcttgatgc ccgcgagttt ttacgcgctg aacgcgccgc acaaccgcag
     5221 gttgatgaag ggctttctta cccgttgctg caacagctac tggtgcaaca tccgggcaaa
     5281 cgtctggtgg tgaccgggtt tatcagccgt aacaacgccg gtgaaacggt gctgctgggg
     5341 cgtaacggtt ccgactattc cgcgacacaa atcggtgcgc tggcgggtgt ttctcgtgta
     5401 accatctgga gcgacgtcgc cggggtatac agtgccgacc cgcgtaaagt gaaagatgcc
     5461 tgtctgctgc cgttgctgcg tctggatgaa gccagcgaac tggctcgtct ggcggctccc
     5521 gttcttcacg cccgtacttt acagccggtt tctggcagcg aaatcgacct gcaactgcgc
     5581 tgtagctaca cgccggatca aggttccacg cgcattgaac gcgtgctggc ctccggtact
     5641 ggtgcgcgta ttgtcaccag ccacgatgat gtctgtttga ttgagtttca ggtgcccgcc
     5701 agtcaggatt tcaaactggc gcataaagag atcgaccaaa tcctgaaacg cgcgcaggta
     5761 cgcccgctgg cggttggcgt acataacgat cgccagttgc tgcaattttg ctacacctca
     5821 gaagtggccg acagtgcgct gaaaatcctc gacgaagcgg ggttacctgg cgaactgcgc
     5881 ctgcgtcagg ggctggcgct ggtggcgatg gtcggtgcag gcgtcacccg taacccgctg
     5941 cattgccacc gcttctggca gcaactgaaa ggccagccgg tcgaatttac ctggcagtcc
     6001 gatgacggca tcagcctggt ggcagtactg cgcaccggcc cgaccgaaag cctgattcag
     6061 gggctgcatc agtccgtctt ccgcgcagaa aaacgcatcg gcctggtatt gttcggtaag
     6121 ggcaatatcg gttcccgttg gctggaactg ttcgcccgtg agcagagcac gctttcggca
     6181 cgtaccgact ttgaatttgt gctggcaggc gtggtggaca gccgccgcag tctgttgagc
     6241 tatgacgggc tggacgccag ccgcgcgtta gccttcttca acgatgaagc ggttgagcag
     6301 gatgaagagt cgttgttcct gtggatgcgc gcccatccgt atgatgattt agtggtgctg
     6361 gacgttaccg ccagccagca gcttgctgat cagtatcttg atttcgccag ccacggtttc
     6421 cacgtcatca gcgccaacaa actggcggga gccagcgaca gcaataaata tcgccagatc
     6481 cacgacgcct tcgaaaaaac cgggcgtcac tggctgtaca atgccaccgt cggtgcgggc
     6541 ttaccgatca accacaccgt gcgcgatctg atcgacagcg gcgatactat tttgtcgatc
     6601 agcgggatct tctccggcac gctctcctgg ttgttcctgc aattcgacgg tagcgtgccg
     6661 tttaccgagc tggtggatca ggcgtggcag cagggcttaa ccgaacctga cccgcgtgac
     6721 gatctctctg gcaaagacgt gatgcgcaag ctggtgattc tggcgcgtga agcaggttac
     6781 aacatcgaac cggatcaggt acgcgtggag tcgctggtgc ctgcccattg cgaaggcggc
     6841 agcatcgacc atttctttga aaatggcgat gaacttaacg agcagatggt gcaacggctg
     6901 gaagcggccc gcgaaatggg gctggtgctg cgctacgtgg cgcgtttcga tgccaatggc
     6961 aaagcgcgtg tgggcgtgga agcggtgcgt gaagaccatc cgctggcatc actgctgccg
     7021 tgcgataacg tctttgccat cgaaagccgc tggtatcgcg ataatccgct ggtgatccgt
     7081 gggcctggcg ctgggcgcga cgtcaccgcc ggggcgattc agtcggatat caaccggctg
     7141 gcacagttgt tgtaatttca gaaatttaat aatgcccggt actcatgttt tcgggtttat
     7201 ggtttctaat gaaatatatt gaattatcat aggattaggc cggattaagc gtttacgacg
     7261 aatccggcaa gaagcaataa gtacatggtt agtttatatt tgcagtccgg tttgctttgc
     7321 ataccggatt ttctttttct taccatcctg aagttttttc atcttccctg atttttcctc
     7381 accatcattg gtcatttttc ggttgacgcc cttcggcttt tccttcatct ttacatctgg
     7441 acgtctaaac ggatagatgt gcacaacaca acatataact acaagcgatt gatgaggtaa
     7501 ggtatgagct tttttcacgc cagccagcgg gatgccctga atcagagcct ggcagaagtc
     7561 caggggcaga ttaacgtttc gttcgagttt ttcccgccgc gtaccagtga aatggagcag
     7621 accctgtgga actccatcga tcgccttagc agcctgaaac cgaagtttgt atcggtgacc
     7681 tatggcgcga actccggcga gcgcgaccgt acgcacagca ttattaaagg cattaaagat
     7741 cgcactggtc tggaagcggc accgcatctt acttgcattg atgcgacgcc cgacgagttg
     7801 cgcaccattg cacgcgacta ctggaataac ggtattcgtc atatcgtggc gctgcgtggc
     7861 gatctgccgc cgggaagtgg taagccagaa atgtatgctt ctgacctggt gacgctatta
     7921 aaagaagtgg cagatttcga tatctccgtg gcggcgtatc cggaagttca cccggaagca
     7981 aaaagcgctc aggcggattt gcttaatctg aaacgcaaag tcgatgccgg agccaaccgc
     8041 gcgattactc agttcttctt cgatgtcgaa agctacctgc gttttcgtga ccgctgtgta
     8101 tcggcgggca ttgatgtgga aattattccg ggaattttgc cggtatctaa ctttaaacag
     8161 gcgaagaaat ttgccgatat gaccaacgtg cgtattccgg cgtggatggc gcaaatgttc
     8221 gacggcctgg atgatgatgc cgaaacccgc aaactggtgg gcgcgaatat tgccatggat
     8281 atggtgaaga ttttaagccg tgaaggagtg aaagatttcc acttctatac gcttaaccgc
     8341 gctgaaatga gttacgcgat ttgccatacg ctgggtgttc gacctggttt ataaatagtg
     8401 tggcttttgt gaaaatcaca cagtgatcac aaattttaaa cagagcacaa aatgctgcct
     8461 cgaaatgagg gcgggaaaat aaggttgtca gccttgtttt ctccctcatt acttgaagga
     8521 tatgaagcta aaaccctttt ttataaagca tttgtccgaa ttcggacata atcaaaaaag
     8581 cttaattacg atcaatttga cctacatctc tttaaccaac aatatgtaag atctcaacta
     8641 tcgcatccgt ggattaattc aattataact tctctctaac gctgtgtatc gtaacggtaa
     8701 cactgtagag gggagcacat tgatgagcac gtcagacgat atccataaca cgacagccac
     8761 tggcaaatgc ccgttccatc agggcggtca cgaccagagc gcgggggcgg gcacaaccac
     8821 tcgcgactgg tggccaaatc aactccgtgt tgacctgtta aaccaacatt ctaatcgttc
     8881 taacccgctg ggtgaggact ttgactaccg caaagaattc agcaaattag attactacgg
     8941 cctgaaaaaa gatctgaaag ccctgctgac agaatctcag ccgtggtggc cagccgactg
     9001 gggcagctac gccggtctgt ttattcgtat ggcctggcac ggcgcaggta cttaccgttc
     9061 aatcgatgga cgcggcggcg cgggtcgtgg tcagcaacgt tttgcaccgc tgaactcctg
     9121 gccggataac gtaagcctcg ataaagcgcg tcgcctgttg tggccaatca aacagaaata
     9181 tggtcagaaa atctcctggg ccgacctgtt tatcctcgca ggtaacgtgg cgctggaaaa
     9241 ctccggcttc cgtaccttcg gttttggtgc cggtcgtgaa gacgtctggg aaccggatct
     9301 ggatgttaac tggggtgatg aaaaagcctg gctgactcac cgtcatccgg aagcgctggc
     9361 gaaagcaccg ctgggtgcaa ccgagatggg cctgatttac gttaacccgg aaggcccgga
     9421 tcacagcggc gaaccgcttt ctgcggcagc agctatccgc gcgaccttcg gcaacatggg
     9481 catgaacgac gaagaaaccg tggcgctgat tgcgggtggt catacgctgg gtaaaaccca
     9541 cggtgccggt ccgacatcaa acgtaggtcc tgatccagaa gctgcaccga ttgaagaaca
     9601 aggtttaggc tgggcgagca cttacggcag cggcgttggc gcagatgcca ttacctctgg
     9661 tctggaagtt gtctggaccc agacgccgac ccagtggagc aactatttct tcgaaaacct
     9721 gttcaaatat gagtgggtac agacccgcag tccggctggc gcaatccagt tcgaagcggt
     9781 agacgcaccg gaaattatcc cggatccatt cgatccgtcg aagaaacgta aaccgacgat
     9841 gctggtgacc gacctgacgc tgcgttttga tcctgagttc gagaagatct ctcgtcgttt
     9901 cctcaacgat ccgcaggcgt tcaacgaagc ctttgcccgt gcctggttca aactgacgca
     9961 cagggatatg gggccgaaat ctcgctacat cgggccggaa gtgccgaaag aagatctgat
    10021 ctggcaagat ccgctgccgc agccgatcta caacccgacc gagcaggaca ttatcgatct
    10081 gaaattcgcg attgcggatt ctggtctgtc tgttagtgag cgggtatcgg tggcctgggc
    10141 atctgcttct accttccgtg gtggcgacaa acgcggtggt gccaacggtg cgcgtctggc
    10201 attaatgccg cagcgcgact gggatgtgaa cgccgcagcc gttcgtgctc tgcctgttct
    10261 ggagaaaatc cagaaagagt ctggtaaagc ctcgctggcg gatatcatcg tgctggctgg
    10321 tgtggttggt gttgagaaag ccgcaagcgc cgcaggtttg agcattcatg taccgtttgc
    10381 gccgggccgc gttgatgcgc gtcaggatca gactgacatt gagatgttcg agctgctgga
    10441 gccaattgct gacggtttcc gtaactatcg cgctcgtctg gatgtttcca ccaccgagtc
    10501 actgctgatc gacaaagccc agcaactgac cctgaccgcg ccggaaatga ccgcgctggt
    10561 gggcgggatg cgcgtactgg gtgccaactt cgatggcagc aaaaacggcg tcttcactga
    10621 ccgcgttggc gtattgagca atgacttctt cgtgaacttg ctggatatgc gttacgagtg
    10681 gaaagcgacc gacgaatcga aagagctgtt tgaaggccgt gaccgcgaaa ccggcgaagt
    10741 gaaatacact gccagccgtg cggatctggt atttggttct aactctgtcc tgcgtgcggt
    10801 ggctgaagtt tacgccagca gcgatgctca cgagaagttt gttaaagact tcgtggcggc
    10861 atgggtgaaa gtgatgaacc tcgaccgttt cgacctgctg taatctgacc ttcttcagcg
    10921 actgcctttc aggcagtcgc tgaagtttct ttaccggcgt atagtgtcca caggaaaact
    10981 acacactgga tctctcatgt ctgccgcagg aaagagcaac ccactggcaa tcagtggcct
    11041 ggttgtgctc acacttatct ggagttatag ctggattttc atgaagcaag tcaccagcta
    11101 catcggtgcc ttcgacttta ccgccttacg ctgcattttc ggcgctctcg ttttattcat
    11161 cgtcctttta ttacgtggtc gcggaatgcg cccgacaccg tttaaataca ccttagccat
    11221 tgccctgtta caaacctgcg ggatggttgg cctggcgcag tgggcgctgg tcagcggcgg
    11281 agcggggaag gtggcgatcc taagctatac catgccgttc tgggtggtga ttttcgccgc
    11341 gttgtttctc ggtgaacgcc tgcgacgtgg gcaatatttc gcgatactga ttgccgcttt
    11401 cggcttattt ttggtgttgc agccgtggca actcgatttc tcttcgatga aaagtgccat
    11461 gctggcaatc ctctccggcg tcagttgggg ggcgagcgcg attgttgcta aacgtctgta
    11521 tgcccgtcat ccgcgcgtgg atttattgtc gttaacatcc tggcagatgc tctatgcggc
    11581 gctggtgatg agtgtggtcg ctttactggt gccgcaacgt gaaattgact ggcagcccac
    11641 cgtgttctgg gcgctggcct acagtgcgat tctggcgacg gcactggcgt ggagcttatg
    11701 gttgtttgta ttgaaaaact tgcctgccag tattgccagc ttaagcacac tggccgttcc
    11761 cgtttgcggc gtactctttt cctggtggct gctcggcgag aatccggggg ccgttgaagg
    11821 tagcggtatt gtgctgattg tgatggcact ggcgctggtg agccgtaaga aaaaagaagc
    11881 cgttagtgta aaaaggatct gaattttttc ttcatgtggg gcgatcgctt atttaacaaa
    11941 ataacgataa tgccccacca tgcgccagct aaacagcaca tcttcttcct gcgcacctgc
    12001 gccaatgtta tgtatcacca gcggcgtacc gtcgcgggcg aagccatctg aaaccacccc
    12061 aatatgtgcc agcccgttat ctaatcgcca ggagacaata tcgcccgctt gatagtcact
    12121 ggggttcttg ctgatggggc gtgttttatc gtggtgggta aaccaggttt ccagattagg
    12181 cacccgacgg tgatcgatgt tgctgtccgg gcgctttaac tgccattttt gcgggtactc
    12241 ggcaaaattc ttcgccatat cttcgtgcac cagtttctgc aaatcgacct tctggctgcg
    12301 caatgcacgg atcaccacat cggaacatac cccgcgtttt tgcgggacat caccgccagg
    12361 ataggtaagc tgcatatacg ccggatcgta aaatagcgtg ctgccgattt gctgtctggc
    12421 accgtctgcg atggcaaggt tggtattggc ctggatttgt accacggttg gtggaacggc
    12481 gggagatttt aaggagtggc tggtaaatgc cgttagcagg ctgagcagcg ccagtgaagc
    12541 tttcatctgg ttcgtccgtg aaaataagag tcacagattc gaccttccgg gataacgatt
    12601 gttctgtaga gaaaatgtcc agaagcggat aaaaaactcc tgaacaacgt gggagtttca
    12661 gattactggc acagagtgcc ggatgcggcg tgaacgcctt atctgtccta aaaaaaaaga
    12721 aaattcaata tattgcagag attgcgtagg cctgataagc gtagcgcatc aggcaatttt
    12781 gcgttcaaac tcccggacaa gccgggagtt tggagtaggt tattcccact cttgcaggaa
    12841 acgctgaccg tactggtcgg ctaccagcag agcggcgtaa acctgatctg gcgtcgcgcc
    12901 gccaggcatg ttgtggatgg tttcacattc tgcacatgcc gcttcagcca caattcgcat
    12961 tttcgccggg acatcttctt taatatccag ttgagcgaga gttattggca aacctaccgc
    13021 atggctaagt gcagctacgg tttcgatttc ctcaaccggt gcgttttcca gaaccagctg
    13081 cgtcagcgta ccaaatgcca ctttttcacc gtgataatag tgatgcgcgt ccgggatagc
    13141 ggtcaggccg ttatgcactg cgtgcgccgc agccagacca ccactttcaa aaccaacacc
    13201 gctcaaatag gtgttcgctt caatcacgcg ctccagcgcc ggagtcacta catgctgttc
    13261 ggcagcaagc atcgcttttt cgccttcttc cagcagggtg ttgtagcaca gttcagccag
    13321 tgccagcgca gcctgggtgc acttgccgcc cgccatggtg gtcgcgccgc tacgagagca
    13381 ggcacgcgct tcaaaccagg ttgccagcgc atcgccgata cccgccgcta acagacgtgc
    13441 aggtgcgcca gcgacgattt tggtgtcgac aatgaccata ttcgggttat ttggcaacag
    13501 cagatagcgg tcaaactcac cctcatcggt gtagataaca gacaatgcgc tgcacggtgc
    13561 atcggtagag gcgatagtcg gtgcgatcgc taccggaaca cccatgaaat gtgccagtgc
    13621 tttggctgta tcgagggttt ttccgccacc gataccgaga attgcgccac actgcgcagt
    13681 ctccgcgatg ccacgcagac ggtcgatctc attttgcgaa catccaccgc caaacggcgc
    13741 aatttctact accagtccag catctttaaa gcttttctcg acagtggatt gagcaaaacc
    13801 taaaacaaat ttgtcaccca ccactaacca gcgttctgcc agcggcttca ggtattcgcc
    13861 cagacgatta atcacatcag cgccctggat gtatttaccc ggtgattgaa taatgcggtc
    13921 cataattgct cctttagaga tgagtagtgc caaatgcggc attccagtcg tgttcgaact
    13981 tctctatagc tgactctacc gcaggggtgt tgagcatttg ttgcgctaca tctaagggca
    14041 gggtgatgga ttcacatcct gccagtaaac agtccagcgc ctgacgcggc gttttaaagc
    14101 tggctgccag caccatgctt tctggcgcgt gcatttctaa cagcgcttgc agctcctgaa
    14161 ccgtacgaat gccgtctccg ccctgggcat ctacgcggtt aacatacgga gcaacgtatt
    14221 ttgcccctgc cagcgcggcc agtaaccctt gtgcagcact gtacactgcg gtgccaagcg
    14281 tagtaatacc ctcttttttc agtattttaa ttgctgccag accttcggaa gtcaccggga
    14341 ttttcaccac aatacccgga atagcgtcgc gcaggcgctt cgcttcttcc accatcccct
    14401 gcgcgtcgcg gctcatggtc tgagcaaaca gaatgccctc atcaccaatc gctttttgca
    14461 gacgcggcag cacttcccat atggactcct tgctggcagc gataatgctc gggttagttg
    14521 tcaccccggc aatggggaat atgcgtgcca gacgttcgac ttctgcgacg ttagcggtgt
    14581 ccagatacag ttccatgatg tttcctttta cagttccagt tcatgttgca gcaggctggc
    14641 gatagcgtcg gcagaggcgg cgttaaccag cgcgttacgg aattcttcgt gcatgatgcg
    14701 gcgagcgagg cgcgagaaaa tgcgcatgtg ctgatcgccc gcagcgtgtt tgttcagggt
    14761 taacatgatg atgaattgcg cttcatcatc accccagcgc accggcgctt gcagacgcgc
    14821 aacgctgatg gtggattgct caatgtgttc tgatttgctg tgtggaatgg caaaactaaa
    14881 gcccagaccg gtagagaaaa cggcctcgcg cgcccacaag tcggcttcca gtttacgcgg
    14941 atagcggcag cgccctgcca gcagcaggtt atcggtcatg cctttgagca cttcttcttt
    15001 gctgcgccag tcgctttcca gtgtgatgca ctcggcggtg accagcggtg cgtcttgttg
    15061 ggtcatgcgg aattgcgcca gcaggtgctc cacttccagc gaagtacggc aggccattgc
    15121 ctggttgagc aacttgcggc actcacggct atcaagttgc gccatccgcg ctttcgccgc
    15181 cggaattgat ggtgcgctca tgctgagttc atccagccct aagccgacca gcaacggcag
    15241 cacggaacct ttcgctccca gctcaccgca cagaccaatc catttaccct ggcgatgcac
    15301 cgcctgcacg gcgtaatcga gcgcccgcaa gaatgccgga ttcaggctgt tgtagtgacg
    15361 agtaacctta gcgttatcgc gatccactgc cagcagatat tgcgtcaggt cattactacc
    15421 aatactaaag aaatcaatct cttcgcagca ttgatcgatg atgaacatca ccgacggcac
    15481 ttccagcata atgccgagct ggatcttctc atcaaacgga atgtgttcgt tacgtagttg
    15541 ctgtttggct tctgccagtt tttctttcac ccataagatc tcttccattg aggagatcat
    15601 cgggatcatg attttcaggc tgccgtgagc ggaggcgcgc aggatcgagc gtagttgtgt
    15661 ggtaaataac gacgcgtatt cttcataaat acgcacggcg cgataaccga ggaacgggtt
    15721 tgcctctgcg ggaatgttta gataatcaac gggtttgtcg ccgccaatgt ccatagtgcg
    15781 cacaataatg ctgcgtccgt tggcagactc cagcgcctga caaaaaatgt tgtacagctc
    15841 gctttcgccc ggtgcactgg tgcgatccat atagagcatt tcagtgcgga acaaaccaac
    15901 gccttccgca ccattgccga atgcggcctg cgcttccacg gagtgagcga tgttagcggc
    15961 aatttcaatg cggataccgt cagcggtacg ggcttgttgg gtcagccaga cacgctgttg
    16021 ctcacgcagg gcgtcctgta cgcgggcttc ttgctgataa taacgagcta cggcttctcc
    16081 tggctcaacc acaatcgccc cggcgttgcc gtcgatataa atcgtttgat gctgccacgg
    16141 agtgagggca tcaatatcca caccaaccag cgtcggaatg ttgaacgaac gggcaaggat
    16201 caccgtatgt gaggtggtgc cgccactttt cagcaacaga cctttgaggt gatttttatc
    16261 cagttcgagg aactggctgg gggtcagttc atcagccata caaatggcgg actgcgtcag
    16321 tttgcccggt gccgggaagc gttgctcacc gtagatttgc tggagtaact ggaagcagac
    16381 gtcgcgtacg tccagggcac gttcttgcag gtagctgctg ctggaacggg cgaactcttc
    16441 gcaaaagtga ttcgcactgg cgacaatcgc ttcggcgcag cttaatccct cgctgacgcc
    16501 tgccagtaag tgctgacgta aagatgtatc gccagccagg gatcggtgag cttccagtat
    16561 cgcgctggta gcaccgtcgc tatccagcag acgaaactca atgtttttca gtaccagcgt
    16621 caggccgttt tccagtgtgg attgctcggc gtcaacgtct ttggctgcgg gaagattact
    16681 cagcgcattg agatctaaag aagagatcgg cgtcagaatg ccgcctgcac taccgctgca
    16741 cacggtgcgg gcgcggataa tttgcggatt cagattggtc agtgaaaccg gcagtggttc
    16801 cagttcgtca gatttaactt ccgccagcgg cgcatcgcag tgggggaatt catcgcgcag
    16861 ccattggctt aaacgctggt gcgcttcctg ttcgtcggcc ccggaaatca gtaactggca
    16921 gttatcgccc gccagcgtat cggtgccaat cagcgcaagg gcgcttttgg cgttgccctt
    16981 gcggtcagtg tgcaggttat gccactcaat ttgtgatgaa aaagtattac acagcgtttc
    17041 aacgtggctt gccggacgcg catgtacgcc gttaggtagc tcacaaataa attccacaat
    17101 cagggccatt gcctctctcc cggtacggtt ttctgtttac aggataagag cgcacggcaa
    17161 cggcctgcca tgtgacaaat ctgccaaaag ctggacaaat gtaatgtaac cgtcaatttg
    17221 cgacgcgtct cacaagacgc cgttttgcgg catgcttccg gtttatcgca agttatgagg
    17281 cggatcgcat ttttgtactg atattacaaa agtccagtaa atggcctttt tatccactgt
    17341 ttgctccgcc tgcgattgcc tattgtttcc tccatcaaaa atatggacat gggctttcgc
    17401 aagcccggga gcagcttatg aatgagttgg tgcagatcct gaaaaatacc cgtcagcatt
    17461 taatgacggg cgtttcacac atgattccct tcgtggtatc gggcggtatt ttgctggcgg
    17521 tttccgtcat gttgtatggc aaaggcgcag tgccggatgc cgtagccgat ccaaatctga
    17581 aaaaactgtt tgatatcggc gttgcgggct tgacgctgat ggtgcctttc ctcgccgctt
    17641 acattggtta ttccattgca gaacgttctg cgctggctcc atgcgctatc ggtacctggg
    17701 ttggtaacag ctttggtgcg ggcttctttg gtgcactgat cgccgggatt atcggcggca
    17761 tcgtggtgca ttacctgaag aaaattccgg tgcataaagt tctgcgctcg gtgatgccta
    17821 tcttcattat tcctatcgtc ggcacactga ttaccgcagg catcatgatg tggggcttgg
    17881 gcgagcctgt aggggcgttg accaacagcc tgactcagtg gcttcagggg atgcagcagg
    17941 gcagcattgt tatgctggcg gtgatcatgg gtctgatgct ggcgttcgat atgggcggtc
    18001 cggttaacaa agtggcctat gccttcatgc tgatttgcgt tgctcagggt gtttataccg
    18061 tggtggccat cgctgccgtg ggtatttgta tcccgccgct ggggatgggg ctggcgacgc
    18121 tgattggtcg taaaaatttc tccgcagaag agcgcgaaac cggcaaagcg gcactggtga
    18181 tgggctgtgt tggcgttact ggaggggcga ttcctttcgc cgctgccgat ccgctgcgtg
    18241 tcattccttc catcatggtc ggttcagttt gtggtgcagt aactgcggcg ctggtcggtg
    18301 cgcagtgcta tgcaggctgg ggtggtctga ttgtgctgcc ggtggttgaa ggcaagctgg
    18361 gttatatcgc agcagtggct gtcggagcag tggtgacggc tgtttgtgtg aacgtgctga
    18421 aaagtctggc gcgtaaaaat gggtcttcga ctgatgaaaa agaagacgac ctggatttgg
    18481 attttgaaat taattaattg aggaacgagc catgacgaaa attattgcag taaccgcatg
    18541 tccttcaggt gttgcccata cctatatggc agcagaggcg ctggaaagtg ccgcgaaagc
    18601 gaaaggctgg gaagtgaaag tagaaaccta ggggtcaatt ggtctggaaa atgaactgac
    18661 tgcggaagat gtggcgagcg ctgatatggt gattctgacc aaagatatcg gcatcaagtt
    18721 tgaagagcgt tttgctggca agaccatcgt gcgcgtcaac atcagcgatg cagtaaaacg
    18781 tgctgacgcc attatgagca aaattgaagc tcacctggcg caaaccgctt aagttcttct
    18841 cccgctcgca agggcgggtt cgctttccca caggagttcc tcatgacgaa tcgtatctct
    18901 cgcctcaaaa ctgcactgtt tgccaatacc cgtgaaatct cgctggagcg ggcgctgctt
    18961 tataccgcca gccatcggca aaccgaaggc gaaccggtga tattgcgccg ggcgaaagca
    19021 acagcgtata tccttgaaca tgttgaaatt tcgattcgtg atgaagaact gattgccggt
    19081 aaccgcaccg taaaaccgcg cgccgggatt atgtcgccgg aaatggaccc ttactggctg
    19141 ctgaaagagc tggatcaatt cccgacgcgt ccgcaggacc gctttgctat cagcgaagaa
    19201 gataaacgta tctaccgtga agagttgttc ccgtactggg aaaaacgttc gatgaaagat
    19261 ttcatcaacg ggcagatgac agatgaagta aaagccgcga ccaacacgca gattttcagc
    19321 atcaaccaga cggataaagg ccaggggcac attattattg attacccacg cctgttgaat
    19381 cacggtctgg gtgagctggt ggcacagatg cagcaacatt gtcagcaaca gccggagaat
    19441 cacttttatc aggccgcgtt gttactgctg gaagcctcgc agaaacacat tttgcgttac
    19501 gccgaactgg cggaaacgat ggcggcaaac tgcacagatg cacagcgtcg cgaagaactg
    19561 ctgactattg cggagatctc tcgccataac gccgaacata agccgcagac gttctggcag
    19621 gcgtgccagt tattctggta catgaacatc attctgcaat acgaatccaa cgccagttcg
    19681 ctgtctttag ggcgcttcga ccagtatatg ttgccgttct atcaggcatc tttaacccag
    19741 ggcgaagatc cggcgttcct gaaagaactg ctcgaatctt tatgggtgaa atgcaacgac
    19801 atcgtgctgt tgcgctccac cagcagcgcg cgttatttcg caggtttccc gaccggctat
    19861 accgcactgc tcggcgggtt aaccgagaac ggacgtagcg cggtgaacgt gctttcgttc
    19921 ctttgccttg acgcctatca aagcgtgcaa ttaccgcaac cgaacctcgg cgtgcgcact
    19981 aacgccttga tcgacacgcc gttcctgatg aaaaccgccg aaaccattcg cctcggcacc
    20041 ggtattccgc aaatctttaa cgatgaagtg gtggtaccag cgttcctcaa ccgtggcgtt
    20101 tcgctggaag atgcgcgcga ctattccgta gtgggctgtg tggaattatc tattcccggc
    20161 agaacctacg gcttgcatga catcgcgatg tttaatctgc tgaaagtgat ggaaatctgc
    20221 ctgcatgaaa atgaaggcaa tgctgcgctg acttatgaag gtttactgga gcagatccgc
    20281 gccaagatca gccactacat caccctgatg gttgagggca gcaatatttg tgatatcggc
    20341 catcgcgact gggcacctgt accgctgctc tcgtctttta tcagcgattg tctggaaaaa
    20401 ggtcgcgata ttaccgatgg cggcgcgcgt tataacttct ccggcgtaca ggggatcggt
    20461 atcgccaacc tgagcgattc tctccatgcg ttgaaaggga tggtttttga gcaacagcgt
    20521 ttaagttttg acgaattgct gtcggtatta aaagccaact ttgcaacgcc agaaggcgaa
    20581 aaagtccgtg ctcgcttaat taaccgcttc gagaaatacg gtaacgatat cgacgaggtg
    20641 gataacatca gcgccgaact gttacgccac tactgcaaag aagtggaaaa ataccagaac
    20701 ccgcgcgcgg ttacttcacg ccgggatcgt ataccgtttc tgctcacgtc ccgttgggat
    20761 cggtggttgg cgcgacgcca gacggtcgtt ttgccgggga acaactggca gacggcggct
    20821 tgtcacctat gctgggccag gacgcacaag ggccaacagc ggtactgaag tcagtcagta
    20881 agctcgataa tacgctgctg tctaacggta cgttgctgaa cgtgaaattc actccggcga
    20941 ccctggaagg tgaagcggga ttacgcaaac tggccgactt cttacgggcg tttacccagc
    21001 ttaagttgca gcatattcag tttaacgtgg tgaacgccga cacgttgcgg gaagcgcaac
    21061 agcgcccaca agattatgcc gggctggtgg tgcgcgttgc cggatacagc gccttctttg
    21121 tcgaactgtc gaaggagatc caggatgaca tcatccgccg gacagcgcat cagctgtaac
    21181 gttgtggaaa cgcgccgcga tgatgtggcg cgcattttca acattcagcg ttattcactg
    21241 aatgacggcg agggcattcg tacggtggtc ttttttaaag gctgtccgca tctttgcccg
    21301 tggtgtgcta atccggagtc gatctccggc aaaatccaga cggtacgcag agaggcgaaa
    21361 tgtctgcact gtgcgaaatg tttgcgtgat gcggatgaat gcccctccgg ggcgtttgaa
    21421 cggattggtc gcgatatcag ccttgacgct ctggaacggg aagtgatgaa agatgacatt
    21481 ttctttcgca cgtccggcgg cggcgtcacg ctttctggcg gcgaagtgtt aatgcaggcg
    21541 gagtttgcta cccgtttttt acagcgactg cggctgtggg gtgtgtcatg cgccattgaa
    21601 actgccggag acgcgccagc aagcaagctg ttaccgctgg cgaaattgtg cgatgaagtg
    21661 ctgttcgatt taaaaattat ggacgcgact caggcgcggg atgtggtgaa gatgaacctg
    21721 ccacgcgtgc tggagaatct gcgtttgctg gtgagtgagg gtgtcaacgt gatcccgcgt
    21781 ttaccgttga tccctggttt cacgctcagc agggagaata tgcagcaggc gctggatgta
    21841 ctgatcccgc tgaaaatcag gcagatccat ctgttaccgt ttcatcagta cggcgaaccg
    21901 aaataccgcc tgctggggaa aacatggtcg atgaaagagg tgcctgcgcc gtcgtcagcc
    21961 gatgtgacaa cgatgcgcga aatggcagaa cgggccggat ttcaggttac cgtgggaggt
    22021 taaaatggca tacctggtgg cagtaaccgc ctgcgtaagc ggcgtggcgc atacttatat
    22081 ggcggcggaa cggctggaaa agttgtgcca gttagagaag tggggcgtca gcattgaaac
    22141 tcagggcgcg ctgggaacgg agaatcgttt agcggacgag gatatccgtc gggcggatgt
    22201 tgctctgttg attacggata tcgagcttgc cggtgccgag cgatttgaac attgccgcta
    22261 tgtgcaatgc agcatctacg cattcctgcg tgagccgcag cgggtaatga gcgcggtgcg
    22321 caaagtgctt tctgcgccgc agcaaaccca tcttattctg gagtagtcgg tttttctgtc
    22381 agttggctgt ggtactgccg gcgatattcc gacggcgagc gttcggtgtt tttacgaaac
    22441 agacggcaga agtagttgct gtcgacaaag ccgcaggcgt gcgccacttc ttttaccttc
    22501 aggtcatagc ctttcaataa cgtcttagcg tgctccagtc gcgtgtgatt caggtattcg
    22561 ttaaaaccaa tggcccccgt tttttgaaac aggtgcgaga gatagtttgg tgaaatatag
    22621 aatgcctgtg cgacagattc gcgggtaagc gcggaggcat agcgttcgtc gatataatcg
    22681 cgaatagctt caaatagtgc ctggctgcgt gaggcggtct ggatttggct gccgagcaaa
    22741 tcgcggcagt ggctaagcag gctggctacg ataagccttg ccgtttgctg ctcctgcggc
    22801 tgcatttgca tttcattgag cgtttgtagc agaaaagaac ctatacgtgg gccgcgtcgg
    22861 gcgacgtgtt gcttcgccag attttgatat tgtttgccat cccattgcac gacgctgaag
    22921 ccgagttgct gtttgccaaa cagcacgcta aaggtggtag cgggtgtttt ccattgtggg
    22981 aaattccagc caccagcggc aacgtacagc acatcgccgg gaactaacgt tgctccagcg
    23041 ccggtatcaa caaactcacc ttccagcaca atttccagcc gtggaaaatc gacctgatac
    23101 gcaagatcgg gcaccgggcc gttactgctg gcaaagtaaa tctggcgcag ggacagcggg
    23161 ccgttgatca ggcgggagag cagatagctg acgccgtgat acatgttatt cccttaggta
    23221 tgtagacggg aaaacagtaa ctggcattat taaaaaaggc cacctgaaaa gtgtggcctg
    23281 aggggagttc gatgtaaaca ttgcggcagt tgtattgccg gatgtggcgt aaacgcctta
    23341 tccagcctac cgccgcgatc tgtaggccgg ataagacgcg tcaagcgtcg catccggcaa
    23401 atagcgcctg gctgataatt actgattacc tacctggtcg ccatacggca gagtgtcgta
    23461 atcgatcagc gcgtttttct tgtacgggtt accaatccag cgggtagttt ctttgaactg
    23521 cggattcacc actgaacggg ttggatcgta accgtcgtaa gataagcccg ccaaatctga
    23581 ccaggtgtgg atcagttccg ccaggctgta tttacgatcg acatcctgtg agaaatcacg
    23641 gggatgagtc gcttgccatt tttccgacgt ccacagcagg aacggaatgg tgtacatgtg
    23701 gcgcgtcggg ttgtcttcgt tacgcccctg agttttgtgc ggcggcgtgt cgtaaacctc
    23761 ttcaccgtgg tcagagaagt aaaccaggaa tccgttcggg tctgccgctt taaagtcttt
    23821 aatcaggctg gcaaccacat gatcgttata caggttagcg ttgtcataat cgttatatga
    23881 ttccagctct tctgcgctta atcctggcgg aacatgatcg gtattgccat caaacttgcc
    23941 ctgattttcc gggtagcggt atttgtattt gatatgcgta cccagcagat gaacgatgat
    24001 cagtttcttc ggcgcagggt cattcagcac atcctggaac ggcttcagca cgttggtgtc
    24061 gtattcacgc gcactctgcg tacgttgctg gttcatgtag tactgcttgt cggtctggcg
    24121 cgaaaatacc gtcagcatgg tattgcgggc ggtcatcgtc tgctggttgg tgatccagaa
    24181 ggttttataa cccgcctgtt tcatcatgtt catcagcgac ggctgcgtca gatacagatc
    24241 cgggttcttt tcattggcaa aggtcagcgc ctgttgcagg atttcaatgg tgtacggacg
    24301 agacgtaact acgttattaa acacggtcag attcggatcg gttttatgca gcgcatccag
    24361 ctccggcgtg gtttcacgcg gataaccgta cagactcatg cgtccgcgct gggtcgactc
    24421 gccaatcacc agcactaaag tgcgcggttc gttacccgat tcatctttga aattagccag
    24481 tggcggcaag gcattatttt cattcagtaa ctttgttagc gagtttagtt gctgacgata
    24541 ctgataatag ccggtcagga attgccacgg tgcggcaggc tccatacgcg aggccaggtt
    24601 atccaacgtt ttctcaaacg gcttgttttt gataaacgta ttcatggcga tcggatgcag
    24661 aatcaagcca taaagcaggg caaaagagac aacataacgc cacggctttg gaatatagac
    24721 cgggcgcagg cgtgtccaca gcagaactgc caccgccgta taggccagcg cgataagcac
    24781 aattttcagg ctgaaatact ggcttaaata ctcgctggct tcgttggtgt tggtttcgaa
    24841 catcacaaac agaacgctct gcgagaactc ctgcccgtag atgacgtagt agcatagcgc
    24901 cgccagagag gccgcccata gcaccacgcc gattactgcg gcaataattt taatccgctt
    24961 cggaaagagg aataccggga tcaaccacag cgagctgaat aacagcgagt cgcgaatgcc
    25021 gttggtgcca ctataaccac tgatgtaaat aatggcctgt agcagagtag agaaaaacca
    25081 aaagtagagc agtgcccaac ccagggcttt ccagctaaaa aggggtttag cctggacttc
    25141 tgtggaatgc atagtaagaa cctgtcttga aaaaaatatc gccgaatgta acgacaattc
    25201 cttaaggata tctgaaggta tattcagaat ttgaataaaa tgcagacaga aatatattga
    25261 aaacgagggt gttagaacag aagtatttca gaaaaccctc gcgcaaaagc acgagggttt
    25321 gcagaagagg aagattagcc ggtattacgc atacctgccg caatcccggc aatagtgacc
    25381 attaacgctt gttcgacgcg aggatccggt tcctggcctt ctttttctgc ctggcgggag
    25441 cggtgcagca actcggcctg caatacgttc agcgggtcgg tgtaaatatt ccgtagctga
    25501 atagactctg caatccacgg cagatcggcc atcagatggg aatcgttggc aatcgccagc
    25561 accactttga tgtcttcttc ttgcaggttg cgtaactctt tacctaacgg ccacagtgct
    25621 ttgtctacca gacgttgatc atagtattcc gccaaccaca ggtctgcttt ggcgaagacc
    25681 atctccagca tgccgagacg cgtcgagaag aatggccaat cgcggcacat ggcttccagc
    25741 tcgctctgtt tgccatcttc gaccactttt tgcagcgccg tacctgcacc cagccaggcg
    25801 gggagcatca ggcggttttg cgtccaggca aaaatccacg gtatggcgcg cagcgactcg
    25861 acgccgccgg ttgggcgacg tttcgccgga cgtgaaccca acggcagttt gcccaattct
    25921 tgttccggcg tagcggagcg gaagtaaggc acaaaatctt tgttttcacg tacgtagcca
    25981 cggtagagat cgcaggagat gactgacagt tcatccataa tgcgacgcca gctctctttc
    26041 ggctcaggtg gtggcagcag gttggcttcc agaatcgccc cggtgtaaag cgacaggctg
    26101 ctgacggtga tttctggcag accatattta aagcggatca tctcgccctg ttcagtcacg
    26161 cgcaggccac ctttcaggct tcctggcggt tgtgacagca gcgccgcatg ggcaggtgcg
    26221 ccgccgcgac caatggaacc gccgcgaccg tggaacaacg tcagctcaat acccgctttt
    26281 tcgcaggttt tgattaatgc atcctgtgcc tgatattgcg cccaggaagc tgccatcacg
    26341 cccgcatctt ttgctgagtc ggaatagcca atcatcacca tctgtttgcc ctgaatcagg
    26401 ccgcgatacc agtcgatatt gagcagctgg gtcatgacat cgttggcgtt gttcaggtca
    26461 tcgagggttt caaacagcgg agcaaccggc atcgcaaacc cgatacccgc ttctttcagc
    26521 agcaggtgga cagccagtac gtcggacggc gttttcgcca tcgagatcac gtaggcggca
    26581 atggagcctt gcggcgcttc ggcaatcacc tggcaggtat cgagcacttc gcgcgtttcg
    26641 gcgcttggtt gccagttgcg cggcagaagc ggacgtttgg agttcagttc gcggatcagg
    26701 aacgcctgtt tgtcggcctc tgaccagctt tcgtagtcgc cgataccgag gtagcgggtc
    26761 agctcgccca gcgcttcggt atggcgggtg ctctcctgac ggatatcaat acggaccagc
    26821 ggtacgccga aacatttcac gcggcgcagg gtgtcgagca gatcgccgtt ggcgataata
    26881 cccatgccac acgcctgaag tgactggtag caagcgtaga gcggttccca cagttcttcg
    26941 ttttgtgtca gcagcccttc tggttttggc agttcttcgc ctttcaggcg cgcttccagc
    27001 catgcctgtg tcgccatcag gcgagaacgc aggtttttca tcagatagcg atacggttct
    27061 gcggcacctt cttcgccaac cagcgccagc agttcagggg tcgcttcaac catcgacagt
    27121 tcagaaacca gaacctgaat atctttcagg aacagatcgg tggctttcca gcgactgagt
    27181 agcaggacgt ggcgggtgat atcggcagtg acgttcgggt tgccgtcgcg gtcaccgccc
    27241 atccacgaag taaaacggac cggaacaaat tcgacgggca gtttgtagcc gaggttctct
    27301 tccagttgtt cgttcagttc gcgcaagtaa ttcggtacgc cttgccacag gctgttttct
    27361 actacggcaa agccccattt ggcttcatct accgggcttg gacgcagctt acggatttca
    27421 tcagtatgcc atgactgggc gattaactgg cgcaggcgac gcatcagctg gttgtgttcg
    27481 tagtcagcga tatctttgtt atcgagctgt tttaagcagg cgttcacttc caccattttg
    27541 tggatcagtg tacgacgggt aatttcggtt gggtgagccg tgaggaccag ttccagcgac
    27601 agcgattcca ctgctttttt gatggtgtct tcgctcagtt ccggctggtt tttcagtttg
    27661 cgcagggtgc gggcgatcac ttccgggttg ctggcagctt cgcctttcgg cgaaatgctg
    27721 tggtattgct cggcggtgtt ggccaggttc aggaactgac taaacgcacg cgcaacgggc
    27781 agcagctcgt cgttcgacaa attttgtaag gtggtgagca actcctggcg gttagcatca
    27841 ttgccagcgc gtgaagattt cgacaactta cggatagttt ctacgcgttc aagaatgtgt
    27901 tctcccaacg catccttgat ggtttctccc agcactttgc cgagcatact gacattacta
    27961 cgcaatgcgg aatattgttc gttcatatta ccccagacac cccatcttat cgtttgatag
    28021 ccctgtatcc ttcacgtcgc attggcgcga atatgctcgg gctttgcttt tcgtcgtctt
    28081 ttataaagcc acgtaaaagc ggtgacgtca aatgctgcga aatcgcttca gcaaacgaat
    28141 aaatagcagg aatttacgcc attaaattca cgacgcttta aataagcgta acttatggaa
    28201 atgttaaaaa atcgccatac ataacaccaa aggtgtaggt cggataagat gcgcaagcat
    28261 cgcatccgac attattgcgg caccggagtt tggcaacggt gccggatgcg gcgcgagcgc
    28321 cttatccggc ctacgtttta atgccagcaa aaatggtgaa ttacctggat aatcaactca
    28381 cgggtgggtt taataaaccg cgtctccaga tattcatcag gctgatgagc ttgattgatg
    28441 gaacccggac cgagcaccag cgtcgggcat aacgtttgaa taaacggcgc ttcggtacag
    28501 taattcacca cttcggtttt tgctccgagc aatttctcaa ccacttcaac cagttgatga
    28561 ttcggtgggc attcatagcc agggatcggc ggatgcagct cgtcgaccgt cagacgaccc
    28621 ggccagcgtt cgctcaccgg agccaatgca tcgttgagca aaccattaag ttcattgagt
    28681 gtcatgctag gcagcggacg aatatccata tgcaactcac agcaagcgca aatacggtta
    28741 gaagcgtcgc caccgtgaat atgcccgagg ttgagcgtag ggtatggcac ggtaaatgct
    28801 tcgtagtgat aacgttcttt caggttatcg cgcaattgca aaatatgccc gatggcgtcg
    28861 tgcattagtt cgatagcgtt aactccgcgt gctggatcgc tggagtgccc cgactggccc
    28921 tgaatacgga tggcgttaga gatatgacct ttatgtgcgc gtaccggttg tagtgacgtc
    28981 ggttcgccaa tgatggcgca atccgggcgc agggcggtag tttcggcaaa ataacgcgct
    29041 ccggccatac tggtttcttc atcagcagtc gccagaatgt agagcggttt tttcagtttc
    29101 gtgacgtcga catcgcgtag cgtatcaagg ataaacgcaa aaaagccttt catgtcggcg
    29161 gtgcctaagc cgtaaagctt gccgtcatgc tccgtcagtg taaacggatc gcgcgtccag
    29221 cgaccgtcat caaatggcac cgtatcggta tgccccgcca gcaacaagcc gccagccccc
    29281 tgtccgatac ttgccagcat attgaatttg ttgcgagttc ctggcacagg ctgcacttcc
    29341 acattgaagc ctaaatcttt aaaccagtcc gccagcagag tgattaaatc tgcattgctt
    29401 tgatcgagtg cctcttccgt ggcgcttatt gaaggtgtgg caatcagagc acggtaaatc
    29461 tcgataaatg gcggtaattt gtttttcatt gttgacacac ctctggtcat gatagtatca
    29521 atattcatgc agtatttatg aataaaaata cactaacgtt gagcgtaata aaacccacca
    29581 gccgtaaggt gaatgtttta cgtttaacct ggcaaccaga cataagaagg tgaatagccc
    29641 cgatgttgaa tacgctgatt gtgggtgcca gcggctacgc tggcgcagag ctagtgacct
    29701 atgtaaatcg ccatccgcat atgaacataa ccgctttgac tgtttcagcg caaagcaatg
    29761 atgcgggaaa gttaatctcc gatttgcatc cgcagctaaa aggcatcgtt gagctgccgt
    29821 tgcagccgat gtcggatatc agcgagttta gcccaggggt ggacgtagtg tttctcgcca
    29881 ccgcccatga agttagccac gatttagcgc cgcagtttct tgaagcgggc tgcgtggtgt
    29941 tcgacctttc cggtgcgttt cgtgttaacg acgccacctt ctatgaaaaa tattacggct
    30001 ttacccatca atacccggaa ctgctggaac aggcagccta cggtctggcg gagtggtgcg
    30061 gtaataaatt aaaagaagcg aatttgattg cggtgccagg ctgttatccg acggcggcac
    30121 agctggcgct gaaaccgttg attgatgccg atcttcttga cctcaatcag tggccggtga
    30181 tcaacgccac cagcggcgtg agcggtgcag gacgtaaggc agcgatttca aatagttttt
    30241 gtgaagttag cctgcaaccg tatggcgtct ttactcatcg ccatcaacca gagatcgcca
    30301 cacacctcgg tgctgacgtt atcttcaccc cacatctggg caatttcccg cgcggcattc
    30361 tcgaaaccat tacctgccgc ctgaaatcgg gtgtgaccca ggcgcaagtc gcgcaagtgt
    30421 tacagcaggc gtatgcccat aaaccgctgg tgcggctgta tgacaaaggc gttccggcgc
    30481 tgaaaaatgt cgttgggctg ccattttgcg atatcgggtt tgccgttcag ggcgagcatc
    30541 tgattattgt ggcgaccgaa gacaacttac tgaaaggcgc ggcggcacaa gcggtacagt
    30601 gcgccaatat tcgtttcggc tatgcggaaa cgcagtctct tatttaaggg tgcaatgatg
    30661 aatccattaa ttatcaaact gggcggcgta ctgctggata gtgaagaggc gctggaacgt
    30721 ctgtttagca cactggtgaa ttatcgtgag tcacatcagc gtccgctggt gattgtgcac
    30781 ggcggcggtt gcgtggtgga tgagctgatg aaagggctga atctgccggt gaaaaagaaa
    30841 aacggcctgc gggtgacgcc tgctgatcag atagacatta tcaccggagc actggcggga
    30901 acggcaaata aaaccctgtt ggcatgggcg aagaaacatc agattgcggc cgtaggtttg
    30961 tttctcggtg acggcgacag cgtcaaagtg acccagcttg atgaagagtt aggtcatgtt
    31021 ggactggcgc agccaggttc gcctaagctt atcaactcct tgctggagaa cggttatctg
    31081 ccggtggtca gctccattgg cgtaacagac gaagggcaac tgatgaacgt caatgccgac
    31141 caggcggcaa cggcgctggc ggcaacgctg ggcgcggatc tgattttgct ctccgacgtc
    31201 agcggcattc tcgacggcaa agggcaacgc attgccgaaa tgaccgccgc gaaagcagaa
    31261 caactgattg agcagggcat tattactgac ggcatgatag tgaaagtgaa cgcggcgctg
    31321 gatgcggccc gcacgctggg ccgtccggta gatatcgcct cctggcgtca tgcggagcag
    31381 cttccggcac tgtttaacgg tatgccgatg ggtacgcgga ttttagctta agttttgttg
    31441 gccggaggcg cagctttccg gcattgaatt tcaaaataag gaaacagagt tatggcactt
    31501 tggggcgggc gttttaccca ggcagcagat caacggttca aacaattcaa cgactcactg
    31561 cgctttgatt accgtctggc ggagcaggat attgttggct ctgtggcctg gtccaaagcc
    31621 ctggtcacgg taggcgtgtt aaccgcagaa gagcaggcgc aactggaaga ggcgctgagc
    31681 gtgctgctgg aagatgttcg cgccaggcca caacaaatcc ttgaaagcga cgccgaagat
    31741 atccatagct gggtggaagg caaactgatc gacaaagtgg gccagttagg caaaaagctg
    31801 cataccgggc gtagtcgtaa cgatcaggta gcgactgacc tgaaactgtg gtgcaaagat
    31861 accgttagcg agttactgac ggctaaccga cagctgcaat cggcgctggt ggaaaccgca
    31921 caaaacaatc aggacgcggt catgccaggt tacactcacc tgcaacgcgc ccagccggtg
    31981 acgttcgcgc actggtgcct ggcctatgtt gagatgctgg cgcgtgatga aagccgtttg
    32041 caggatgcgc ttaagcgtct ggatgtcagc ccattaggct gtggcgcgct ggcgggaacg
    32101 gcctatgaaa tcgaccgtga acagttagca ggctggctgg gctttgcttc ggcgacccgt
    32161 aacagtctgg acagcgtttc tgaccgtgac catgtgttgg aactgctgtc ggctgccgct
    32221 atcggcatgg tgcatctgtc gcgttttgct gaagatctga ttttctttaa caccggcgaa
    32281 gcggggtttg tggagctttc tgaccgcgtg acttccggtt catcattaat gccgcagaag
    32341 aaaaacccgg atgcgctgga gctgattcgc ggtaaatgcg gtcgggtgca gggtgcgtta
    32401 accggcatga tgatgacgct gaaaggtttg ccgctggctt acaacaaaga tatgcaggaa
    32461 gacaaagaag gtctgttcga cgcgctcgat acctggctgg actgcctgca tatggcggcg
    32521 ctggtgctgg acggcattca ggtgaaacgt ccacgttgcc aggaagcggc tcagcagggt
    32581 tacgccaacg ccaccgaact ggcggattac ctggtggcga aaggcgtacc tttccgcgag
    32641 gcgcaccata tcgtgggtga agcggtggtg gaagccattc gtcagggcaa accgctggaa
    32701 gatctgccgc tcgacgagtt gcagaaattc agcccggtga ttggcgaaga tgtctatccg
    32761 attttgtcgc tgcaatcgtg cctcgacaag cgtgcggcaa aaggcggcgt ctcaccgcag
    32821 caggtggccc aagcgattgc ttttgcgcag gcgcggttgg ggtaagaaca tttatatgta
    32881 caaatttgag cctggcttat cgccgggctt ttttatggca aaaaaaagcg gatcctggag
    32941 atccgcaaaa gttcacgttg gctttagtta ttcgagttga gaaactctcg aaacgggcag
    33001 tgacttcaag ggttaaaaga ggtgccgctc cgtttctgtg agcaattatc agtcagaatg
    33061 cttgataggg ataatcgttc attgctattc tacctatcgc catgaactat cgtggcgatg
    33121 gaggatggat aatgaatatt cgtgatcttg agtacctggt ggcattggct gaacaccgcc
    33181 attttcggcg tgcggcagat tcctgccacg ttagccagcc gacgcttagc gggcaaattc
    33241 gtaagctgga agatgagctg ggcgtgatgt tgctggagcg gaccagccgt aaagtgttgt
    33301 tcacccaggc gggaatgctg ctggtggatc aggcgcgtac cgtgctgcgt gaggtgaaag
    33361 tccttaaaga gatggcaagc cagcagggcg agacgatgtc cggaccgctg catattggtt
    33421 tgattcccac ggttggaccg tacctgctac cgcatattat cccgatgctg caccagacct
    33481 ttccaaagct ggaaatgtat ctgcatgaag cacagaccca ccagttactg gcgcaactgg
    33541 acagtggcaa actcgattgc gtgatcctcg cgctggtgaa agagagcgaa gcattcattg
    33601 aagtgccgtt gtttgatgag ccaatgttgc tggctatcta tgaagatcac ccgtgggcga
    33661 accgcgaatg cgtaccgata gccgatctgg caggggaaaa actgctgatg ctggaagatg
    33721 gtcactgttt gcgcgatcag gcaatgggtt tctgttttga agccggggcg gatgaagata
    33781 cacacttccg cgcgaccagc ctggagacgc tgcgcaacat ggtggcggca ggtagcggga
    33841 tcactttact gccagcgctg gctgtgccgc cggagcgcaa acgcgatggg gttgtttatc
    33901 tgccgtgcat taagccggaa ccacgccgca ctattggcct ggtttatcgt cctggctcac
    33961 cgctgcgcag ccgctatgag cagctggcag aggccatccg agcaagaatg gatggctatt
    34021 tcgataaagt gttaaaacag gcggtttaaa ccgtttaacg cagctacccg ataggcttcc
    34081 gccatggtcg ggtagttaaa ggtggtgttg acgaagtact caatagtgtt gccgccacct
    34141 ttctgttcca taatcgcctg accgatatga ataatttcgg cagcgcgctc gccaaagcag
    34201 tgaataccca gaatctcttt tgtttcccga tggaacaaaa ttttcagcgt gcccacgttc
    34261 atgccgacga tttgtgcgcg tgccagatgt ttaaactggg cgcggcccac ttcatatggc
    34321 actttcatcg cggtcagctg ctgttcggtt ttgcccacag agctgatttc cgggatggtg
    34381 taaatgccgg tagggatatc ttcaatcaga tgcgcggtgg cttcgccttt taccagcgcc
    34441 tgcgcggcaa tgcgcccctg gtcataggcc gctgatgcca ggctcggata accaatcacg
    34501 tcgcccaccg cgtaaacgtg cggctgtgcg gtctgataca tgctgttgac cttcagctgt
    34561 ccgcgactgt cagtttccag cccaatgttc tgtaacgcca gcgaatcggt attaccggta
    34621 cgaccgttgg catagagcag gcagtcagct ttcagttttt tacccgactt cagatgcatg
    34681 atcacaccgt cgtcacagcc ttcgatcttc tcgtactctt cgttgtgacg aatcactacg
    34741 ccactgttcc agaagtgata ggagagagaa tctgacatct cttgatcgag aaatgccagc
    34801 aggcgatcgc gggtgttgat cagatccact tttacatcca taccgcggaa gatcgacgca
    34861 tattcacagc cgatcactcc agcaccatag ataagtacat ggcgcggttc gtggtgcatg
    34921 ctaagaattg agtcgctgtc gtaaatgcgt ggatgggtga aatcaacatc tgttggatga
    34981 tatggacgag agccgcaggc aataacaaat ttttcagcgg ttagtgtttc gacgctgccg
    35041 tccgggcaat ccagcgccaa cgtatgctcg tcaacaaagc gagcgttccc ctgcaatatt
    35101 tcacagtgat tacgttcgta aaatccctga cgcatgcgcg tttgttgatt aatcacgtta
    35161 tcggcatggt taaggatatc ggcaaaagaa gagcggagca gtcgcgaatg gtcgctgtat
    35221 agtgggtttt gattgaattc tataatgcgg ctgacggcgt gacggagagc tttcgacggg
    35281 atggtgcccc agtgggtgca accgccgcca acattttgat aacgctcgat aactgcgacg
    35341 cgcacacctt gcttaaccag gcccattgca gcgccttcgc cgccggggcc ggaacctatt
    35401 actatggcat cgtaatcgta ggaatgtggc atggtagggc ttacctgttc ttatacataa
    35461 aagcaacaga atggtaacat tctatcgcgg gtaagccaat tgatccccgt catttatctg
    35521 gctatatcct gagcggcctt tgctttgtct gtttcttact tttgccctga cgttttattg
    35581 gatttttatc gacgatactc tccgtttaag cggcaggttt ccgctgtacg taaaagaacc
    35641 ggccagtaaa tatgttttat tgcgttaccg ttcattcaca atactggagc aatccagtat
    35701 gttcattctc tggtatagtg ccagcagtac ttttggcaag gattcagaca tcgtgatggg
    35761 cataagagcg caacaaaaag aaaaaacccg ccgttcgctg gtggaagccg catttagcca
    35821 attaagtgct gaacgcagct tcgccagcct gagtttgcgt gaagtggcgc gtgaagcggg
    35881 cattgctccc acctcttttt atcggcattt ccgcgacgta gacgaactgg gtctgaccat
    35941 ggttgatgag agcggtttaa tgctacgcca actcatgcgc caggcgcgtc agcgtatcgc
    36001 caaaggcggg agtgtgatcc gcacctcggt ctccacattt atggagttca tcggtaataa
    36061 tcctaacgcc ttccggttat tattgcggga acgctccggc acctccgctg cgtttcgtgc
    36121 cgccgttgcg cgtgaaattc agcacttcat tgcggaactt gcggactatc tggaactcga
    36181 aaaccatatg ccgcgtgcgt ttactgaagc gcaagccgaa gcaatggtga caattgtctt
    36241 cagtgcgggt gccgaggcgt tggacgtcgg cgtcgaacaa cgtcggcaat tagaagagcg
    36301 actggtactg caactgcgaa tgatttcgaa aggggcttat tactggtatc gccgtgaaca
    36361 agagaaaacc gcaattattc cgggaaatgt gaaggacgag taatgaaaca agcaaatcaa
    36421 gatagaggta cgctgctgct ggcgttagtt gctggcttat cgattaatgg tactttcgca
    36481 gcgctgttta gctccattgt gccattttct gtattcccga ttatttccct ggtgctgacg
    36541 gtttactgcc tgcatcaacg ttatcttaat cgcactatgc cggtaggctt gccgggtctg
    36601 gcagctgcct gttttattct cggcgtactg ctgtacagca cggtagttcg tgcggaatat
    36661 ccggatatcg gctctaactt cttcccggca gtactctccg tcattatggt gttctggatt
    36721 ggcgcgaaga tgcgtaaccg taagcaggaa gttgctgagt aatcggtatt atgccggatg
    36781 ctggcgcatc cggcatgggt tttacttcgc ggtcagtaat acgccgcact ccatatggtg
    36841 cgtgtagggg aactgatcaa acagagccag acgttcgacc ttgtgcgtct ggcttaatgt
    36901 ttccagattc ttgcataacg tttccgggtt acaggagatg tacaaaatac acggatacgc
    36961 ctgcaccatt ttctcggttt cactgtccag accgctgcgc ggtgggtcga caaaaatggt
    37021 ttcgcactga taactcttta agtcgatccc ttgcaggcgg ttaaactcgc gcacaccatt
    37081 catcgcctga gtaaattctt ctgccgccat acgaataatt tgtacgttat caatatggtt
    37141 agctgcgatg ttgtattgcg cagcagcaac cgacggctta gcgatttcgg tggctaatac
    37201 ccgatcaaaa ttgcgcgcca gcgctaatga aaagttaccg ttgccgcagt acagctccag
    37261 taaatcgcct tttgagcctt tggttacgtc cagcgcccat tccagcatct gaatattcat
    37321 cgccgcgttc ggctgggtaa agctgttttc tacctgacgg tagatcatct ctttccctgc
    37381 gaccggcaga cgctcgtcga tgtaatcttg atccagctcg attttggttt tcgttgcccg
    37441 accaatcaga tgcacattca gattctgcgc gcgcagtgca tcgcgcaggg cctccgcttc
    37501 ctgacgccac tcatcatcca gcttcttatg gtatagcagg gaaaccaccg cctgattact
    37561 cagcgtggtg aggtaatcaa tctggaacaa cttgtggcgc agaacgggat tattacgcac
    37621 acccgcaatc atcgccgtca tcaactggtt gataagttca ctggcggcgg ggaagctatc
    37681 cacgcggata cggcttttgg tttgttgatc gaaaatgatg tgatacaggt catcgccatc
    37741 gtgccagatg cggaactccg cacgcatccg gtaatgactg accggcgagc gaaacacttc
    37801 cggaaccagg tcagaaaacg gtgccatcat actttgcaaa cgtaccactt tttcggctaa
    37861 ctgcgcttca tactgttctg ttggaaggtg ttcgggggtc atgatgtatc ctgaaaaatt
    37921 aaagtacgcg gcgattgtag ggattgctca tcagatgtcc agatcttgat gaattcctat
    37981 ttgtgagcta cgtctggaca gtaacttgtt acaacctgta gcatccactt gtcggtcctg
    38041 tgagttaata gggaatccag tgcgaatctg gagctgacgc gcagcggtaa ggaaaggtgc
    38101 gatgattgcg ttatgcggac actgccatcc ggtgggaagt catcatctct tagtatctta
    38161 gatacccctc caagcccgaa gacctgccgg ccaacgtcgc atctggttct catcatcgcg
    38221 taatattgat gaaacctgcg gcatccttct tctattgtgg atgctttaca atgattaaaa
    38281 aagcttcgct gctgacggcg tgttctgtca cagccttttc cgcttgggca caggatacca
    38341 gcccggatac tctcgtcgtt actgctaacc gttttgaaca gccgcgcagc actgtgcttg
    38401 caccaaccac cgttgtgacc cgtcaggata tcgaccgctg gcagtcgacc tcggtcaatg
    38461 atgtgctgcg ccgtcttccg ggcgtcgata tcacccaaaa cggcggttca ggtcagctct
    38521 catctatttt tattcgcggt acaaatgcca gtcatgtgtt ggtgttaatt gatggcgtac
    38581 gcctgaatct ggcgggggtg agtggttctg ccgaccttag ccagttccct attgcgcttg
    38641 tccagcgtgt tgaatatatc cgtgggccgc gctccgctgt ttatggttcc gatgcaatag
    38701 gcggggtggt gaatatcatc acgacgcgcg atgaacccgg aacggaaatt tcagcagggt
    38761 ggggaagcaa tagttatcag aactatgatg tctctacgca gcaacaactg ggggataaga
    38821 cacggggaac gctgttgggc gattatgccc atactcatgg ttatgatgtt gttgcctatg
    38881 gtaatacagg aacgcaagcg cagacagata acgatggttt tttaagtaaa acgctttatg
    38941 gcgcgctgga gcataacttt actgatgcct ggagcggctt tgtgcgcggc tatggctatg
    39001 ataaccgtac caattatgac gcgtattatt ctcccggttc accgttgctc gatacccgta
    39061 aactctatag ccaaagttgg gacgccgggc tgcgctataa cggcgaactg attaaatcac
    39121 aactcattac cagctatagc catagcaaag attacaacta cgatccccat tatggtcgtt
    39181 atgattcgtc ggcgacgctc gatgagatga agcaatacac cgtccagtgg gcaaacaatg
    39241 tcatcgttgg tcacggtagt attggtgcgg gtgtcgactg gcagaaacag actacgacgc
    39301 cgggtacagg ttatgttgag gatggatatg atcaacgtaa taccggcatc tatctgaccg
    39361 ggctgcaaca agtcggcgat tttacctttg aaggcgcagc acgcagtgac gataactcac
    39421 agtttggtcg tcatggaacc tggcaaacca gcgccggttg ggaattcatc gaaggttatc
    39481 gcttcattgc ttcctacggg acatcttata aggcaccaaa tctggggcaa ctgtatggct
    39541 tctacggaaa tccgaatctg gacccggaga aaagcaaaca gtgggaaggc gcgtttgaag
    39601 gcttaaccgc tggggtgaac tggcgtattt ccggatatcg taacgatgtc agtgacttga
    39661 tcgattatga tgatcacacc ctgaaatatt acaacgaagg gaaagcgcgg attaagggcg
    39721 tcgaggcgac cgccaatttt gataccggac cactgacgca tactgtgagt tatgattatg
    39781 tcgatgcgcg caatgcgatt accgacacgc cgttgttacg ccgtgctaaa cagcaggtga
    39841 aataccagct cgactggcag ttgtatgact tcgactgggg tattacttat cagtatttag
    39901 gcactcgcta tgataaggat tactcatctt atccttatca aaccgttaaa atgggcggtg
    39961 tgagcttgtg ggatcttgcg gttgcgtatc cggtcacctc tcacctgaca gttcgtggta
    40021 aaatagccaa cctgttcgac aaagattatg agacagtcta tggctaccaa actgcaggac
    40081 gggaatacac cttgtctggc agctacacct tctgaaccac gtcccaccgt gctggtgttt
    40141 gactccggcg tcggtgggtt gtcggtctat gacgagatcc ggcatctctt accggatctc
    40201 cattacattt atgctttcga taacgtcgct ttcccgtatg gcgaaaaaag cgaagcgttt
    40261 attgttgagc gagtggtggc aattgtcacc gcggtgcaag aacgttatcc ccttgcgctg
    40321 gctgtggtcg cttgcaacac tgccagtacc gtttcacttc ctgcattacg cgaaaagttc
    40381 gacttcccgg ttgttggtgt cgtgccggcg attaaacctg ctgcacgtct gacggcaaat
    40441 ggcattgtcg gattactggc aacccgcgga acagttaaac gttcttatac tcatgagctg
    40501 atcgcgcgtt tcgctaatga atgccagata gaaatgctgg gctcggcaga gatggttgag
    40561 ttggctgaag cgaagctaca tggcgaagat gtttctctgg atgcactaaa acgtatccta
    40621 cgcccgtggt taagaatgaa agagccgcca gataccgttg tattgggttg cacccatttc
    40681 cctctactac aagaagaact gttacaagtg ctgccagagg gaacccggct ggtggattct
    40741 ggcgcagcga ttgctcgccg aacggcctgg ttgttagaac atgaagcccc ggatgcaaaa
    40801 tctgccgatg cgaatattgc cttttgtatg gcaatgacgc cagaagctga acaattattg
    40861 cccgttttac agcgttacgg cttcgaaacg ctcgaaaaac tggcagtttt aggctgattt
    40921 ggttgaatgt tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg ccggaataac
    40981 tccctataat gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg
    41041 gttctcctga gaatctcaac agagaaaagc aaaaaaatgc ttgactctgt agcgggaaag
    41101 cgtattatgc acaccccgcg ccgctgagaa aaagcgaagc gg
//
LOCUS       DAETQN010000020        41013 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.020, whole genome shotgun sequence.
ACCESSION   DAETQN010000020 DAETQN010000000
VERSION     DAETQN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.020"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            216..1880
                     /gene="asnB"
                     /locus_tag="K0W68_001133"
     CDS             216..1880
                     /gene="asnB"
                     /locus_tag="K0W68_001133"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBI3072759.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2260..2336
                     /locus_tag="K0W68_001134"
     tRNA            2260..2336
                     /locus_tag="K0W68_001134"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2294..2296,aa:Met,seq:cat)
     gene            2345..2429
                     /locus_tag="K0W68_001135"
     tRNA            2345..2429
                     /locus_tag="K0W68_001135"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2379..2381,aa:Leu,seq:tag)
     gene            2453..2527
                     /locus_tag="K0W68_001136"
     tRNA            2453..2527
                     /locus_tag="K0W68_001136"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2485..2487,aa:Gln,seq:ttg)
     gene            2562..2636
                     /locus_tag="K0W68_001137"
     tRNA            2562..2636
                     /locus_tag="K0W68_001137"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2594..2596,aa:Gln,seq:ttg)
     gene            2652..2728
                     /locus_tag="K0W68_001138"
     tRNA            2652..2728
                     /locus_tag="K0W68_001138"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2686..2688,aa:Met,seq:cat)
     gene            2777..2851
                     /locus_tag="K0W68_001139"
     tRNA            2777..2851
                     /locus_tag="K0W68_001139"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2809..2811,aa:Gln,seq:ctg)
     gene            2888..2962
                     /locus_tag="K0W68_001140"
     tRNA            2888..2962
                     /locus_tag="K0W68_001140"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2920..2922,aa:Gln,seq:ctg)
     gene            complement(3116..4291)
                     /gene="ubiF"
                     /locus_tag="K0W68_001141"
     CDS             complement(3116..4291)
                     /gene="ubiF"
                     /locus_tag="K0W68_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBI3072760.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4437..5861
                     /gene="miaB"
                     /locus_tag="K0W68_001142"
     CDS             4437..5861
                     /gene="miaB"
                     /locus_tag="K0W68_001142"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBI3072761.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            6014..7054
                     /locus_tag="K0W68_001143"
     CDS             6014..7054
                     /locus_tag="K0W68_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBI3072762.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            7051..7518
                     /gene="ybeY"
                     /locus_tag="K0W68_001144"
     CDS             7051..7518
                     /gene="ybeY"
                     /locus_tag="K0W68_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBI3072763.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7608..8486
                     /gene="corC"
                     /locus_tag="K0W68_001145"
     CDS             7608..8486
                     /gene="corC"
                     /locus_tag="K0W68_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBI3072764.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8511..10049
                     /gene="lnt"
                     /locus_tag="K0W68_001146"
     CDS             8511..10049
                     /gene="lnt"
                     /locus_tag="K0W68_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBI3072765.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(10127..12004)
                     /locus_tag="K0W68_001147"
     CDS             complement(10127..12004)
                     /locus_tag="K0W68_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBI3072766.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            12459..13367
                     /gene="gltI"
                     /locus_tag="K0W68_001148"
     CDS             12459..13367
                     /gene="gltI"
                     /locus_tag="K0W68_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBI3072767.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            13537..14277
                     /gene="gltJ"
                     /locus_tag="K0W68_001149"
     CDS             13537..14277
                     /gene="gltJ"
                     /locus_tag="K0W68_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBI3072768.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            14277..14951
                     /gene="gltK"
                     /locus_tag="K0W68_001150"
     CDS             14277..14951
                     /gene="gltK"
                     /locus_tag="K0W68_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBI3072769.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            14951..15676
                     /gene="gltL"
                     /locus_tag="K0W68_001151"
     CDS             14951..15676
                     /gene="gltL"
                     /locus_tag="K0W68_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBI3072770.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            15794..16729
                     /gene="rihA"
                     /locus_tag="K0W68_001152"
     CDS             15794..16729
                     /gene="rihA"
                     /locus_tag="K0W68_001152"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBI3072771.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            16821..18491
                     /gene="hscC"
                     /locus_tag="K0W68_001153"
     CDS             16821..18491
                     /gene="hscC"
                     /locus_tag="K0W68_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HBI3072772.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(18551..20005)
                     /gene="djlC"
                     /locus_tag="K0W68_001154"
     CDS             complement(18551..20005)
                     /gene="djlC"
                     /locus_tag="K0W68_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBI3072773.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDDEEEREAFLEA
                     IQAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAK
                     LHRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLC
                     AYWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTR
                     LLWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVV
                     LEAFPKQEMLARLNTRLEGGDTHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKN
                     DEDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(20002..20709)
                     /locus_tag="K0W68_001155"
     CDS             complement(20002..20709)
                     /locus_tag="K0W68_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBI3072774.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            20811..21365
                     /locus_tag="K0W68_001156"
     CDS             20811..21365
                     /locus_tag="K0W68_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBI3072775.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(21375..22802)
                     /locus_tag="K0W68_001157"
     CDS             complement(21375..22802)
                     /locus_tag="K0W68_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBI3072776.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(22799..23506)
                     /locus_tag="K0W68_001158"
     CDS             complement(22799..23506)
                     /locus_tag="K0W68_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBI3072777.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            23670..24646
                     /locus_tag="K0W68_001159"
                     /pseudo
     CDS             23670..24646
                     /locus_tag="K0W68_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
     gene            complement(24716..25198)
                     /locus_tag="K0W68_001160"
     CDS             complement(24716..25198)
                     /locus_tag="K0W68_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBI3072778.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25433..28015
                     /gene="leuS"
                     /locus_tag="K0W68_001161"
     CDS             25433..28015
                     /gene="leuS"
                     /locus_tag="K0W68_001161"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBI3072779.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            28030..28611
                     /gene="lptE"
                     /locus_tag="K0W68_001162"
     CDS             28030..28611
                     /gene="lptE"
                     /locus_tag="K0W68_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBI3072780.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            28611..29642
                     /gene="holA"
                     /locus_tag="K0W68_001163"
     CDS             28611..29642
                     /gene="holA"
                     /locus_tag="K0W68_001163"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBI3072781.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            29644..30285
                     /gene="nadD"
                     /locus_tag="K0W68_001164"
     CDS             29644..30285
                     /gene="nadD"
                     /locus_tag="K0W68_001164"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBI3072782.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30309..30920
                     /locus_tag="K0W68_001165"
                     /pseudo
     CDS             30309..30920
                     /locus_tag="K0W68_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            31180..31497
                     /gene="rsfS"
                     /locus_tag="K0W68_001166"
                     /gene_synonym="rsfA"
     CDS             31180..31497
                     /gene="rsfS"
                     /locus_tag="K0W68_001166"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBI3072783.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31501..31968
                     /gene="rlmH"
                     /locus_tag="K0W68_001167"
     CDS             31501..31968
                     /gene="rlmH"
                     /locus_tag="K0W68_001167"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBI3072784.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            31999..33900
                     /gene="mrdA"
                     /locus_tag="K0W68_001168"
     CDS             31999..33900
                     /gene="mrdA"
                     /locus_tag="K0W68_001168"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBI3072785.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            33903..35015
                     /gene="mrdB"
                     /locus_tag="K0W68_001169"
                     /gene_synonym="rodA"
     CDS             33903..35015
                     /gene="mrdB"
                     /locus_tag="K0W68_001169"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBI3072786.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            35026..36114
                     /gene="rlpA"
                     /locus_tag="K0W68_001170"
     CDS             35026..36114
                     /gene="rlpA"
                     /locus_tag="K0W68_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBI3072787.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36253..37464
                     /gene="dacA"
                     /locus_tag="K0W68_001171"
     CDS             36253..37464
                     /gene="dacA"
                     /locus_tag="K0W68_001171"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBI3072788.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            37574..37837
                     /locus_tag="K0W68_001172"
     CDS             37574..37837
                     /locus_tag="K0W68_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBI3072789.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            37938..38579
                     /gene="lipB"
                     /locus_tag="K0W68_001173"
     CDS             37938..38579
                     /gene="lipB"
                     /locus_tag="K0W68_001173"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBI3072790.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            38839..39792
                     /locus_tag="K0W68_001174"
     CDS             38839..39792
                     /locus_tag="K0W68_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBI3072791.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            40001..40966
                     /gene="lipA"
                     /locus_tag="K0W68_001175"
     CDS             40001..40966
                     /gene="lipA"
                     /locus_tag="K0W68_001175"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBI3072792.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT        10299 a        10449 c        10291 g         9974 t
ORIGIN      
        1 ccccgtaaaa tataaccatt caataaaacc cgctaaaaat tatcgattca tcaataaata
       61 catcctcaca ccagaccctt tttcaccaat cgacaatcac cattacgttt tttatttttt
      121 caccgccaaa tcgcttgcgc acagtgctgc tttgcggcat ttttttaaac aagcaaacac
      181 aacaagcaac aaataccagg ttaacggaga aggttatgtg ttcaattttt ggcgtattcg
      241 atatcaaaac agacgcagtt gagcagcgta agaaagcact cgagctgtca cgcctgatgc
      301 gtcatcgtgg cccggactgg tccggtattt atgccagcga taacgccatt ctcgctcacg
      361 aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc tctctacaac caacaaaaaa
      421 cccacgtact ggcggtaaac ggtgaaatct acaaccacca ggcattgcgc gctgaatatg
      481 gcgatcgtta tcagttccag acagggtctg actgtgaagt gatcctcgcg ctgtatcagg
      541 aaaaagggcc ggaatttctc gacgacttgc agggcatgtt tgcctttgcc ctgtacgaca
      601 gcgaaaaaga tgcctacctg attggtcgcg accatctggg gatcatccca ctgtatatgg
      661 ggtatgacga acacggtcag ctgtatgtgg cctcagaaat gaaatcgctg gtgccagttt
      721 gccgcacgat taaagagttc ccggcgggga gctatttgtg gagccaggac ggcgaaatcc
      781 gttcttatta tcatcgcgac tggttcgact ttgatgcagt gaaagataac gtgaccgaca
      841 aaaacgagct gcgtcaggct ctggaagatt ccgttaaaag ccatctgatg tctgatgtgc
      901 cttacggtgt gctgctttct ggtggtctgg attcctcaat tatttccgct atcaccaaga
      961 aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga agcctggtgg ccccagttac
     1021 actcctttgc tgtaggtctg ccgggttcac cggatcttaa agcagcccag gaagtggcaa
     1081 accatctggg cacggtgcat cacgaaattc acttcactgt acaggaaggt ctggatgcca
     1141 tccgcgacgt gatttaccac atcgaaactt atgatgtgac cactattcgc gcttcaacac
     1201 cgatgtattt aatgtcgcgt aagatcaagg cgatgggcat taaaatggtg ctgtccggtg
     1261 aaggttctga tgaagtgttt ggcggttatc tttacttcca taaagcgccc aacgctaaag
     1321 aactgcatga agagacggtg cgtaaactgc tggccctgca tatgtatgac tgcgcgcgcg
     1381 ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt tccgttcctc gacaaaaaat
     1441 tccttgatgt ggcgatgcgc attaacccgc aggataaaat gtgcggtaac ggcaaaatgg
     1501 aaaaacacat cctgcgtgaa tgttttgagt catacctgcc cgcaagcgtg gcctggcggc
     1561 agaaagagca gttctccgat ggcgtcggtt acagttggat cgacaccctg aaagaagtgg
     1621 cggcgcagca ggtttctgat cagcaactgg aaactgcccg cttccgcttc ccgtacaaca
     1681 cgccaacctc aaaagaagcg tatctgtacc gggagatctt tgaagaactg ttcccgcttc
     1741 cgagcgccgc tgagtgcgtg ccgggcggcc cgtccgtcgc gtgttcttcc gctaaagcga
     1801 ttgagtggga tgaggcgttc aagaaaatgg acgatccgtc tggtcgtgcg gttggcgttc
     1861 accagtcggc gtataagtaa gaaatgcgaa acagccccgg tgaaatccgg ggctgtttca
     1921 gttattgagg tgcttgttga ttctgatatg cagtaaggaa atgttccggg tttgccacat
     1981 cactgtttga caactcatca ctgtaggcca gtatttcata gattaatttc ccaccaccat
     2041 tgttgaattc cgaatttcat cctgtatgac aaaagaatgg ctgtagggtg gggaaaaaac
     2101 acaaagtaaa caataattga cgaatatagc gccacgctgt tcgcaaccta accaaacagt
     2161 cactttcgag caattttcct tgaaaaagag gttgacgctg caaggctcta tacgcataat
     2221 gcgccccgca acgccgataa ggtatcgcga aaaaaagatg gctacgtagc tcagttggtt
     2281 agagcacatc actcataatg atggggtcac aggttcgaat cccgtcgtag ccaccatctt
     2341 ttttgcggga gtggcgaaat tggtagacgc accagattta ggttctggcg ccgcaaggtg
     2401 tgcgagttca agtctcgcct cccgcaccat tcaccagaaa gcgttgtacg gatggggtat
     2461 cgccaagcgg taaggcaccg gtttttgata ccggcattcc ctggttcgaa tccaggtacc
     2521 ccagccatct tcttcgagta agcggttcac cgaccggtta ttggggtatc gccaagcggt
     2581 aaggcaccgg tttttgatac cggcattccc tggttcgaat ccaggtaccc cagccatcga
     2641 agaaacaatc tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc
     2701 acaggttcga atcccgtcgt agccaccaaa ttctgaatgt gtcgaatacg ttcggcaaat
     2761 tcaaaaacca atttgttggg gtatcgccaa gcggtaaggc accggattct gattccggca
     2821 ttccgaggtt cgaatcctcg taccccagcc aatttattca agacgcttac atcgtaagtg
     2881 caccagttgg ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt
     2941 tcgaatcctc gtaccccagc cacattaaaa aagctcgctt cggcgagctt tttgcttttc
     3001 tgcgttcatt caatgtcgaa tgcgatgttg acacgtctta tccttcaatg tcggatgcga
     3061 cgctgccgcg tcttatccga cctacggttg gcacgcatcc ggcaatgttg taaggctaca
     3121 accctaacgc atatttcagc gcctgacgtt tcaacacgcc agcacgctct gccgccatta
     3181 acccgagatt acgcacaaaa cgcagcggcg gcagattatt actgaatccg gcataaaaca
     3241 gatccatacc actttgcata atgaagttat ccgccatgcg ccgcatctgg taacgcttga
     3301 ggacaggata actggcccac gcttcaccgt agctgcgggc attcaccaga acatcaatca
     3361 gggcatcgac atcacgataa ccaagattca ccccctgccc cgccagcgga tggatggtat
     3421 gcgcggcatc gcccaccagc gcaagccccg gctgcacata ctgcaacgca tgtcggcgcg
     3481 tcagcggaaa cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac gggaaatgct
     3541 tcgcgatttc cgcctggaat tgcgccatat tcatattctg caactggcga atacgcgccg
     3601 gagagtcata ccacaccagc gatgcccagt tatcaaacaa cggcagaaac gcacgcggtc
     3661 cgtccggggt aaattgctgc caggtgctgt cgccgggatc gttctcacac tgtacgctaa
     3721 tcaacatgca cgactgcgca tactgccagg catgaacgcc aattcccgcc atctgccgca
     3781 cctgagaatt tgcgccgtcg gcaccaatca ccagtttcgc gcggatcgtt tcaccgccct
     3841 tcagttccag ctcctgaaga tcattatctc gatgtaatgc aatcagcgag cctggcacac
     3901 gtaacgttac tttcggatgc gcttccagcg cctgccacag cgcctgttgc aggacggtgt
     3961 tttccaccat atagccaagc aacggtagct taagttcagc ggcgtcaaac accacatgcg
     4021 ccgtttccca ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtactg
     4081 catcccagac ccctaaccct ttaagcaatg ataccgatgc ggcgctgatc gccgagatcc
     4141 gcacgtccgg ttggctatca gcgacaaacg gtgcaggttc tgcgtgctcg atcaccgtta
     4201 ccgaaaatcc gtgctgtgcc agccccagcg ccagcgcgcc gccgaccatt cctccgccga
     4261 caatggcaat ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt
     4321 aagtttacag gatttttacc cccaagggct gataacgctc atactggtca caacggcagc
     4381 aaagcattac actatacgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga
     4441 ccaaaaaact ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga
     4501 tggccgatct gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg
     4561 atgtgctgct gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc
     4621 agttgggtcg ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggtg
     4681 gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta
     4741 tttttgggcc gcaaacgctg caccgtctgc cggagatgat caactccgtg cgcggcgacc
     4801 gcagcccggt tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctaccggaac
     4861 cgcgcgccga agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca
     4921 cctactgcgt ggtgccttac acccgtggtg aagaggtaag ccgtccgtct gacgatattc
     4981 tgtttgagat tgcccagctt gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga
     5041 acgtgaacgc ctggcgtggt gaaaactacg acggctccac cggatcgttt gccgatctgc
     5101 tgcgtctggt tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga
     5161 tcgaattcac cgacgatatc atcgaagtgt atcgcgacac gccggagctg gtgagcttcc
     5221 tgcatctgcc agtacaaagc ggttccgatc gcattctgaa cctgatgggc cgtactcaca
     5281 cggcgctgga gtacaaagcg atcatccgta aactgcgtgc ggcgcgtccg gatattcaga
     5341 tcagttctga cttcattgtt ggcttccctg gcgaaaccac cgaagacttc gagaaaacga
     5401 tgaagctgat tgccgacgtc aatttcgaca tgagctacag ctttatcttc tctgcacgtc
     5461 cgggtacacc agccgccgat atggttgatg atgttccgga agaagagaag aagcaacgtc
     5521 tgtatattct gcaagaacgt atcaatcagc aagcgatggc atggagccgc cggatgctcg
     5581 gcaccaccca gcgtattctg gtagaaggga cttcgcgtaa gagcatcatg gagctttccg
     5641 ggcgtaccga aaataaccgc gtggtcaact tcgagggcac accggatatg atcggtaaat
     5701 tcgtcgatgt agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgta
     5761 ctgaagatga aatgggtctg cgcgtggcag aaacaccgga atcggtgatt gcccgtaccc
     5821 gcaaagaaaa cgaccttggc gtgggttatt atcagccgta attctcaggc ccgccgttcc
     5881 ggtgggcctt gttttttctc tctttatccc catctttatc gcaatgcttg cgcctttttc
     5941 ctgtggcgtg aataatttcc ttataggccg gtgaatgttc agcttccggc ggcatacaga
     6001 taagaggaac ggtttgaaca tagacactcg cgaaatcacc ctggagccag cagacaacgc
     6061 gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc aagcagctcg aacgccgtct
     6121 cggcatcgag atcaatcgcc gcgacaacca ctttaaactg accggccgtc cgatttgcgt
     6181 caccgctgcg gcagacattc tgcgtagcct gtatgtcgat acagccccga tgcgcggtca
     6241 gattcaggat atcgaaccgg aacagatcca ccttgcgatt aaagaagcgc gagtactgga
     6301 gcaaagtgcg gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa ccaaacgcgg
     6361 cgtaattaag ccgcgcacgc caaaccaggc gcagtacatc gccaatattc tcgaccatga
     6421 cattaccttc ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tggctgcggc
     6481 agttgatgcc ctggagcgtc aggagattcg ccgtattctg ctgactcgtc cggcggtcga
     6541 agccggtgag aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtatct
     6601 gcgcccactg tacgacgcgc tgtttgaaat gctgggcttt gagaaagttg agaaactgat
     6661 tgagcgcaac gttattgaag tcgcgccgct ggcctatatg cgtggtcgta cgctgaacga
     6721 cgcgtttatc attctcgatg agagccagaa caccaccatc gaacagatga agatgttcct
     6781 gacccgtatc ggttttaact caaaagcggt tatcaccggc gacgtcacac agatcgacct
     6841 gccgcgtaat actaaatcag gcttacgtca cgccatcgaa gtgctggccg atgtcgaaga
     6901 gatcagcttt aacttcttcc acagcgaaga cgtggttcgt caccctgtgg tggcgcgtat
     6961 cgttaacgcc tatgaagcct gggaagaagc cgaacaaaaa cgaaaagcgg cgctggcggc
     7021 agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg
     7081 gcatgtgaag ataattccgg gttaccggaa gagagccagt ttcaaacatg gctgaatgcg
     7141 gtgatcccgc agtttcagga agaatcggaa gtgactattc gcgtggtcga taccgccgaa
     7201 agccacagtc tgaatctgac ctatcgcggt aaggataagc cgaccaacgt gctctccttc
     7261 ccgtttgaag tgccgcctgg catggaaatg tcgctactgg gcgatctggt tatctgccgt
     7321 caggtggttg agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca ctgggcgcat
     7381 atggtggtgc acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca
     7441 gaagaaatgg aagccctcga aacagagatt atgcttgctc tgggctatga ggatccgtac
     7501 attgccgaga aagaataatt tgccaggcat ttgactggca gacgtccccg ccgttggtga
     7561 ttaacgacga cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca
     7621 attcacacag tagtgacacg ataagcaaca agaagggatt tttctccctg ttactcagcc
     7681 aacttttcca cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg
     7741 ggcagaacga ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg
     7801 cagaccaacg cgttcgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca
     7861 accagacgct ggacgaatgc cttgatgtca tcatcgagtc cgcccactca cgtttcccgg
     7921 tgattagcga agacaaagat cacattgaag ggattctgat ggcgaaagac ttgctgccgt
     7981 ttatgcgcag cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcggttgtcg
     8041 ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca
     8101 tggcgatcgt cattgacgaa ttcggtgggg tttccggtct ggtgaccatt gaagacatcc
     8161 tggaactgat tgttggtgag attgaagacg agtatgacga agaagatgat atcgacttcc
     8221 gtcagctgag tcgtcatacc tggaccgtgc gtgcactggc ttccattgaa gacttcaacg
     8281 aagcgttcgg cacccacttt agcgatgaag aggttgacac tatcggtggt ctggtgatgc
     8341 aggcatttgg gcatcttccg gcgcgtggcg aaactatcga catcgacggt taccagttca
     8401 aagtggcgat ggccgacagt cggcgtatta ttcaggttca tgtcaaaatc ccggatgact
     8461 caccccagcc gaagctggat gaataaaacc gaaactggat agataactac atggcttttg
     8521 cctcattaat tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg
     8581 gaacgctggc cttctctcct tacgacgtct ggcctgcggc gattatttcg ttgatggggc
     8641 ttcaggcgtt gacctttaac cgccgtccac tccagtctgc cgctattggc ttttgctggg
     8701 gatttggcct ctttggcagc ggtattaact gggtctatgt cagcatcgcg acctttggcg
     8761 gaatgcctgg cccggttaac atcttcctgg tggtgctgct ggcggcgtat ttgtcgctgt
     8821 ataccggact gtttgctggc gtgctgtcgc gtctgtggcc gaaaaccacc tggctgcgcg
     8881 tggcgattgc cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt tgggtactga
     8941 caggcttccc gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg
     9001 caccgataat gggcgtggaa gccattaact tcctgctgat gatggttagt ggcctgctgg
     9061 cactggcgtt ggtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg ctgtttgccc
     9121 tacccttccc gctgcgttac atccaatggt ttaccccgca accggagaaa accattcagg
     9181 tttcgatggt tcagggcgat attccgcaat cactgaaatg ggatggagat cagcttctta
     9241 atacgctgaa gatttactac aacgcaacgg ctccgctgat gggcaaatca tcgttgatta
     9301 tctggccgga gtcggcgata accgatctgg aaattaatca gcaaccgttc ctcaaagcac
     9361 tggacggtga gttgcgcgat aaaggtagct cgctggtaac cgggattgtc gacgcgcgtc
     9421 tcaataagca gaaccgctac gatacctaca acaccatcat cacactgggt aaaggtgcac
     9481 catacagcta cgaatcagcc gatcgctata acaaaaacca tctagtgccg tttggcgagt
     9541 ttgtcccgct ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg ccgatgtcgt
     9601 cgttcagccg tgggccatat atccagccgc cgctgtcggt aaatggtatt gagcttactg
     9661 cggctatttg ctacgagatc attctcggcg agcaagtgcg cgataacttc cgcccggata
     9721 ccgactatct gctgactatc tccaacgatg cgtggtttgg taagtctatt ggtccatggc
     9781 agcacttcca gatggcgcga atgcgtgcgc tggagctggc gcgcccactg ttgcgcagca
     9841 ccaacaacgg cattacggcg gtgattggcc cgcaggggga ggttcaggcg atgatcccgc
     9901 agttcacccg cgaggtgttg accactaatg tgacaccgac caccggactc acaccgtatg
     9961 cacgtaccgg caactggcca ctgtgggtgc tgacggcgct gtttggtttt gccgctgtgt
    10021 tgatgagtct gcgagcacgt aaacgttgat gtgcctgatg tatcctgcat gagcaaaacg
    10081 tactttctca ttgagctttg gggcatggtt atcatgcccc tttttcttac ggttcaggga
    10141 tacaagccac attgccatat tggggatgac gaataattgc ctgcggatca agcgagcgcg
    10201 tcctgtccat ttggatcaac ccgttcttcg ccatgacaaa tatttttcta ccatccgctg
    10261 aaaaacgaag gtgattaacg tagctggcag gataaacgcc aataatctgc ccggcattaa
    10321 tattgagcag gatgacataa ccttgcaggc catatgacac caacgctaaa tattgaccgt
    10381 ccgggctaat accctcaacg gaataaacgc tcctgctaca ccattgcgtc ataatgctgg
    10441 cttgttgatc ccctacgctt atacgtgaaa tcccctgact atcggcaaaa ataacctcgc
    10501 cgttttcagg tcgaaaacgc atcgccgata tcgttccacg cgcatcacct gcgggaaagt
    10561 cttctttctg atgcgtgatg gtattgatcc gcacgatgtt acctctgcct gctcccggaa
    10621 ccagtaatgt tctttcatcc ggggaaagtg ccataaccca ggcgcctggt ttaccgctcg
    10681 tccccgtccc gtcgtatttc tccagcggga tatccgccac atgctgataa gtcaccagat
    10741 caatcgctgg cacactatta ctggtgcgat tcatcaccca ggcttttgtg ccatcacgcg
    10801 agaggataag gctgtcagga tgtgcaccgg tggtaatcga ctgaataatc tcccctgtcg
    10861 ccacgttaat gaccgataac gcattcgctt caaatgaagt cgcataaatc aacttttcat
    10921 ccgggcttaa tgccagcgat ctgacgccct gatctttaca gccaccacaa tgtttgactg
    10981 acttttcttt cgctatgggc gctgtaaagg tatgcaggat tttcttttgt ccgaggtcga
    11041 aaacaacaat gctgttcgtg ttttcaattg cggcatacaa catattacca tcccgactga
    11101 tcgccatatc atggatatcg gcaatctcag cgagcaaatc accactggcc tgttcacgac
    11161 ttaccggagc ggataatgtt tccctgaggg cgttgcgttc ctccgctaac tgctgctttt
    11221 tttgccgtcg ctcttgttca atctcctgtg gataaaacgc ttcacgcagg ctttgcctga
    11281 cctgtaaaac tgactcatcc atctgctgct gcgcaagcca gatagccccg gtcagaagaa
    11341 gactgcccgc aacaataatc ccgccttccg taacgagtcg gttttgccca gataaacgcg
    11401 cgctcagcca acccaacgcg ccacccgcaa tcaggccgcc aatgtggcaa gcgttatcta
    11461 ttcctgtttg ccgggcaccg tacaacagtg tcagcgccac catacccaac agaggaaata
    11521 ctcgccgctg gtttttatgg tatgtgcccg tacctgaagc aaactgtgtt gctatcgctg
    11581 caccagctat tcccagaatc gccccggatg ccccaacgct gatgactatc tgctcacttt
    11641 cgcgtaacgc ataacaggcg ctgatgagcc caccaaagac gccggagaat aaccaggtaa
    11701 tcaggagtcg ccatttcccc agaatctgtt ccgccacgcc tcccacgacc aggaatgcaa
    11761 acatattcat cagcagatga gaaacactac tgtgcagaaa gatactgctc aataagcgcc
    11821 agggttcacc cgacaaagta agaggcgcaa cgtttgcgcc gagatggata agtgcggatt
    11881 gatcgacctc ttcataactc atccattgtg cgagataaca aaatatccca atacagagtg
    11941 caaaaaggat aagggttatt gcgggtaatt gaacatttaa cggcttaacc gaagatgcag
    12001 acataaggtg tttcttcctt gaaaagacag gtaaaaagca tttttcccgt gtgcagttat
    12061 catcccggca aacgtcccat gccgaaaaga taattctgtc cgaaatatga ctttttcagc
    12121 ctaaaattac aatcgtaatt aattcgcccc taagtatttt ttggcatgta agttgcttta
    12181 tttactgtgt aagcgatgaa atcagccgcg acgcaaccag gttgcaccac cacaaatcag
    12241 cggtagcacc aaaaaggtgc ggtttaagaa ttttgcttcg gaatggtgcg gcgcatctcg
    12301 caaaaacaaa caataaaaca gcaatatctt tacatttagc caaactaaat gttacctaac
    12361 aggcacaaca ctgcacaata aagttgcaga cgataacaac acaaacactc acaacgggta
    12421 tccatgcgtt cttaacgcag aagataaagg agttggatat gcaattacgt aaacctgcca
    12481 cagcaatcct cgccctggcg ctttccgcag gactggcaca ggcagatgac gccgccccgg
    12541 cagcaggcag tacgctggac aaaatcgcca aaaacggtgt gattgtcgtc ggtcaccgtg
    12601 aatcttcagt acctttctct tattacgaca atcagcaaaa agtggtgggt tactcgcagg
    12661 attactccaa cgccattgtt gaagcagtga aaaagaaact caacaaaccg gacttgcagg
    12721 taaaactgat tccgattacc tcacaaaacc gtattccact gctgcaaaac ggcactttcg
    12781 attttgaatg tggttctacc accaacaacg tcgaacgcca aaaacaggcg gctttctctg
    12841 atactatttt cgtggtcggt acgcgcctgc tgaccaaaaa gggcggcgat atcaaagatt
    12901 ttgccgacct gaaagacaaa gccgtagtcg tcacttccgg cactacctct gaagttttgc
    12961 tcaacaaact gaatgaagag caaaaaatga atatgcgcat catcagcgcc aaagatcaca
    13021 gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg gatgatgccc
    13081 tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa ctgggaaatc gtcggcaagc
    13141 cgcagtctca ggaagcctac ggttgcatgt tgcgtaaaga cgatccgcag ttcaaaaagc
    13201 tgatggatga caccaccgcg caggtgcaga cctccggtga agcggaaaaa tggtttgata
    13261 aatggttcaa aaatcccatt ccgccgaaaa acctgaacat gaatttcgaa ctgtcagacg
    13321 aaatgaaagc actgttcaaa gaaccgaatg acaaggcact gaactaatta caagaaccag
    13381 gggcggaaat tccagccctc tcgattgtta cgtagcacgg acagactata cgcctgatgg
    13441 tcgttcccca tcgggcctga aaaccgcaat acgctgggta acaatcttcg agggtagcag
    13501 ttaacgctgc taccctcttt tttctggagt agatttatgt ctatagactg gaactggggt
    13561 atttttttac aacaagcccc gttcggcaac accacctatc ttggttggat ctggagtggt
    13621 tttcaggtga cgatcgcttt gtcgatctgc gcctggatta tcgctttcct cgtcggttca
    13681 tttttcggca ttttacgtac cgttccaaac cgttttctct ccggtctggg tacgttgtat
    13741 gtcgaactgt tccgcaacgt gccgttgatt gtgcaattct ttacctggta tctggtgatc
    13801 ccggagctgt tgccggagaa aatcggcatg tggtttaagg ccgagctgga tcccaatatt
    13861 cagtttttcc tttcatccat gctctgcctg gggctgttta ccgccgcccg tgtttgcgaa
    13921 caggtacgcg ccgccattca gtcgttgccg cgtgggcaaa aaaatgccgc gctggcgatg
    13981 ggcctgacgc taccgcaggc ttatcgatac gtactgctgc ctaatgctta tcgcgttatc
    14041 gtcccgccga tgacttccga gatgatgaac ctggtgaaaa actccgccat cgcctcgact
    14101 attggtctgg tggatatggc ggcgcaggcg ggtaaattgc ttgattactc agcccacgcc
    14161 tgggagtcgt ttaccgctat tacgctggcg tatgtgttga ttaacgcggt tatcatgctg
    14221 gtaatgacgc tggttgaacg taaagttcgc ctgcctggca acatgggggg caaataatgt
    14281 acgagtttga ctggagttcc attgtccctt ccctgccata tctgctcgac gggctggtga
    14341 tcacgctgaa aatcaccgtc acggcagtcg tgattggtat tttgtggggc accatgctgg
    14401 cggtgatgcg cttgtccagc tttgcgcccg tagcgtggtt tgccaaagcc tacgttaacg
    14461 tattccgctc gattccttta gttatggttt tgctgtggtt ttacctgatc gtgccaggtt
    14521 ttctgcaaaa cgtgctggga ttatcaccaa aaaatgatat ccgtttaatt tcggcgatgg
    14581 tggcgttttc gatgtttgaa gcggcctact attcagagat tatccgtgct ggtattcaaa
    14641 gtatttctcg tggtcagtcg agcgccgcgc tggcgttggg aatgactcac tggcagtcga
    14701 tgaaactgat tattctgccg caggcgttcc gcgcgatggt gccgcttctg ctcactcagg
    14761 gcatcgtact gttccaggat acctcactgg tgtatgtgtt atctctggcc gatttcttcc
    14821 gtaccgcctc aaccattggt gagcgtgatg gtacgcaggt tgagatgatc ctgtttgccg
    14881 gatttgttta tttcgttatt agtcttagcg cgtcgttgtt ggtcagctac ttgaaaagaa
    14941 ggacagcata atgattaccc tgaaaaatgt ttcaaaatgg tatggtcact ttcaggtgct
    15001 gaccgactgc tcaaccgaag tgaaaaaagg cgaagtggtg gtggtttgcg gcccgtctgg
    15061 ttccggcaaa tcaacgctga ttaaaaccgt caacggcctc gaaccagtgc agcaaggtga
    15121 aatcaccgtc gatggtatcg tggttaatga caagaaaacc gatctggcaa agctgcgttc
    15181 ccgcgtcggg atggtattcc agcatttcga gctgttccct catctgtcga ttatcgaaaa
    15241 cctgaccctg gcgcaggtga aagtgcttaa acgcgataaa gcgcccgctc gtgaaaaagc
    15301 attgaaattg ctggagcgcg tgggactttc cgcacatgcc aataaatttc cggcgcagct
    15361 ttccggcggt cagcagcagc gtgtggcaat cgctcgcgca ttgtgtatgg atcctattgc
    15421 gatgctgttc gacgaaccga catcggcgct ggatccggag atgatcaacg aagtgctgga
    15481 cgtgatggtg gaactggcga acgaaggaat gaccatgatg gtggtgaccc acgaaatggg
    15541 ctttgcccgc aaagtggcga atcgggtgat ctttatggac gagggtaaaa ttgtcgaaga
    15601 ctcgccgaaa gacgctttct tcgatgatcc gaaatcggac cgcgcgaaag acttcctcgc
    15661 gaaaatcctg cattaatcac tctggcgcgc atacccaagt gcgcgccatc tcacgcttta
    15721 tcgtttcgcc cctcgtatct gttacatcct ggcgttaccc ttgtcgcaaa gaagcacaca
    15781 acaaggagca acaatggcac tgccaattct gttagattgc gacccaggtc atgacgacgc
    15841 tatcgcaata gttctcgccc tcgcctcacc agagcttgat gtcaaagcaa ttacgtcttc
    15901 cgccggaaac cagacaccag aaaaaacctt acgcaatgtt ctgcgtatgc tgaccttgct
    15961 taatcgcacc gatattccgg tagcaggcgg cgcggtaaaa ccgttaatgc gtgagttgat
    16021 tatcgcggac aatgtgcacg gcgaaagcgg tctggatggc ccggcattac cggaaccgac
    16081 gttcgcaccg caaaactgta cggcggtaga gctgatggcg aaaacgctgc gcgaaagtgc
    16141 ggaacctgtc acgattgtgt ctaccggacc gcaaactaac gttgccttgc tgctcaatag
    16201 ccatccggaa ctgcatagca aaattgcccg tatcgtgatt atgggcggcg caatggggct
    16261 tggtaactgg acgcctgcgg ctgaatttaa tatttacgtt gacccggaag cggcagaaat
    16321 tgtcttccag tcagggatcc cagtggtgat ggccggtctg gatgttactc acaaagcaca
    16381 aatccacgtt gaagacaccg agcgtttccg cgcgattggc aaccctgttt caaccattgt
    16441 tgccgaactg ctggatttct tcctcgaata tcataaagac gaaaaatggg gctttgtcgg
    16501 tgcaccgctg catgacccat gcaccatcgc ctggctgttg aaacccgagt tgtttaccac
    16561 tgttgagcgc tgggttggcg tggaaacaca ggggaaatat agccagggta tgacggttgt
    16621 tgattattat tatctgactg gcaataaacc gaatgccact gtaatggtcg atgttgatcg
    16681 tcagggcttt gttgatttac tggctgatcg tctgaaattt tacgcttaac acacgtcatt
    16741 gattaagatc accacgcgtt acctgggtgg tcttaatctg tttatctgtt tatctctttt
    16801 tctttcaaga tataaatgca atggataatg cagaactcgc cattggtatc gatctcggca
    16861 ctaccaatag tttaattgcc gtctggaaag acggtgccgc gcaattaatt ccaaataagt
    16921 tcggtgaata tttaacacca tccataatta gcatggatga aaataatcat attttagtcg
    16981 gaaaaccggc tgtatcacgg cgtacttcgc atccggataa aacggcagcg ttatttaaac
    17041 gtgcaatggg cagtaatacc aactggcggt taggcagcga cacatttaac gcgccagaac
    17101 tgtcctcttt ggtattacgc tcattaaaag aagatgccga agaatttctg caacgtccga
    17161 ttaaagatgt ggtgatctcc gttccggctt atttcagcga tgaacaacgc aagcataccc
    17221 gtttagcagc ggagttagcc gggttaaatg cggtacgctt aattaatgaa cccacagcag
    17281 ctgcgatggc atatggcctg catacgcaac aaaatacccg ctcgctggtt tttgatctcg
    17341 gtggcggcac gtttgacgtc acggtgctcg aatacgccac gccggtgatt gaagttcacg
    17401 cctccgctgg cgataacttc ctcggtggcg aagattttac ccatatgttg gtcgatgacg
    17461 ttttaaagcg cgcggatgtc gccaggacca cgcttaacga gagtgaactg gcagccttgt
    17521 acgcctgtgt ggaagcggca aaatgtagca atcaatcgcc attgcacatt cgctggcagt
    17581 accaggatga aacgcgggag tgcgaatttt acgagaacga actggaagat ttgtggttgc
    17641 cgctgctcaa tcgcttgcga gtgccgattg aacaggcgtt gcgcgatgca cgtctgaagc
    17701 cgagtcaaat cgacagtctg gtgctggttg gcggcgcgtc acaaatgccg ctggtgcagc
    17761 gaatcgccgt gcgtctgttt ggcaaattac cgtatcaaag ttacgacccg agcaccattg
    17821 tcgcgctggg cgcagcaatc caggccgcct gccgcttacg cagtgaagat attgaagagg
    17881 tgatcctcac tgatatttgc ccttactcgt tgggcgttga agttaaccgc cagggcgttt
    17941 ccggcatttt ctcgccaatt attgaacgaa acaccacagt acccgtgtcg cgtgtagaaa
    18001 cttactcgac catgcacccg gaacaggatt ctattacggt taacgtctat cagggagaaa
    18061 accacaaagt taaaaataac attctggttg aatccttcga tgtgccgttg aagaaaaccg
    18121 gggcttatca gtcgattgat attcgcttta gttatgatat caacggcttg cttgaagttg
    18181 acgtgcttct ggaagatggc agcgttaagt ccagagtcat taatcatagc ccggtaacat
    18241 tgagcgcgca gcagattgaa gagagtcgga cgcggttatc cgcattgaaa atttatccgc
    18301 gcgatatgct aatcaaccgc acctttaagg ccaaactgga agagttgtgg gcgcgggcgc
    18361 tgggtgacga gcgagaagag attggccggg tgatcaccga ttttgatgcg gcgttgcagt
    18421 caaacgatat ggcccgcgtc gatgaagttc gtcgacgggc gagcgattat ttggctattg
    18481 agatccctta aacacggctg ccggatgcgg cataaacgct gcatccggca tcggcgcaaa
    18541 aaacacatcc tcagctaaac aacatcgcga tttttccgag cgcataacct atcatcagca
    18601 ccgttaagct gattttgata atactggtaa tgctctgtgg tttttcatcc tcatcatttt
    18661 tttcttcatg gttttcaggt gttggctcat cgacatcttc atcctccggc tcgagtttca
    18721 ctttcgtcaa cagcatctgg gtgataatgg cctctaatgg atgagtatct ccaccttcca
    18781 gccttgtatt aagacgggcc agcatctctt gtttcgggaa cgcttccagc acaacctgtt
    18841 ccgctacccg atacaaatcc agtaacggtg aatcttgctc tgtctcgcta ccgactctcg
    18901 atttccacca cgcccatgct ttgcgcttga agaataacga ctcaataaac gattgaccta
    18961 acggcgataa ttttccgcta tgtaaaatct ccgcccataa cagccgcgtc cgggcgctct
    19021 caccaggaac atatttcaac ggtttacctt gcgcatctac catgcttcgc gcttcgataa
    19081 ccatcactaa taacgcgaaa taatccgggc aatgacgctt gctccactgc aacaacaaat
    19141 tatcagcttg tgtagatggg taactttccc agtacgcgca gagatcggca agcaggcttt
    19201 ccccctcgcc gcagtacaaa cgttgtgcac actggtaata ccgggcatct tcgctttctg
    19261 gttcttccgc ttgccactgt ttggctacag gaatgagttc cgccatacct gtttgcaccg
    19321 agctgtacca gcgcaacagt ttgcgatgta actttgcatc atcaggaatg aaccacggtc
    19381 cttccatcgc caaaaacgca gtgacatagt caggatggta acggcagcaa cgttccagcg
    19441 cgaagtaata ttcaacagtc tggttctgta acgcgacggg atattccaga aggctaagga
    19501 aatcgaaaca atcaccagcc tgaatggcct caagaaaagc ctccctttct tcctcatcat
    19561 ccattccttc gctgtcatta aacgaatgcc agttcaaacg ctctgccaac aaagaagcac
    19621 aatttaatga aagatatcgc gcttctatag cgattgcaca cagcggccag cgtaattgat
    19681 cgacatcctc catgttccag gtatttaatt gctgaataaa tttctgccag gcggagggct
    19741 gaaaacgatc actttctgaa tccagtaatg tcctgaatgc acgtagtatt tcatgttcag
    19801 cggtggcatc tttttcttca tcatcggctt cctcgacagg atttaccgcc agtcgcagcg
    19861 cctcttcata ggcctggcgt aatgctttaa acccctgcgg atcggtttcg ggatgacaca
    19921 acggtaagcg agcaagataa gcctggcgga taatgtctat ttgcgtcgtg ctttcaattt
    19981 cgaggatttg ccaacatgtt ttcataggtc gctcacatcc ttaagcgtta ccgggcattc
    20041 aggctggaca gttaatggtt gccagggaag cgtggcatag aaacggtcgc ttcccgctgc
    20101 cagctcttcg aacatccgac tcccggcgct atcgtatttg tacagggtta gcgcctcgcg
    20161 tagcgaagcg ttgtcttcgc actgagaaga ttgccagtag agacgcccta gtgaataggc
    20221 agaaaaatag tgcatccagc tgtgatagta atggtgagct cgtgcataga cgcgcgattg
    20281 cagccataaa ctctcttctt ccgtcagcca gttgttgagc acaccgaccc gtgacagaaa
    20341 ccccattcga acataatccc aggctttgat tcccccttct ccacagcaca tagcggtttg
    20401 ggcaacgaat tgggtataaa tcaaccctct ctccgagcca ccagcaatta atgctttcct
    20461 ctcttcaggg gaagagcgtt gccatgtcag atataagcca gctaaatcat tggcatgacc
    20521 atcatcagtc atctgataaa tagtctgaat taaatcatca cgattattta ttccccagga
    20581 tgatgagaga tcgaattctt ttatcccagt atacattttt ggcgagtcat gcgcgggttt
    20641 gcaactctga ttaagtattt ccatgggcgc agaaagcgca aaaagaagat attgttcttc
    20701 cttattcatt ttttaataat cccttaagtg cattaactga ctactttttt attattgatg
    20761 agtataaact tgacgctttc aaaataaaaa gaaaatcgaa gcattcacac atgaataaaa
    20821 aatcaatgta tatattcgca atttttatag ttgcagcaat tacctgtatt agccaaccca
    20881 agaaaacgac gttgcgtgat aaagccatgg tgaattatgc ctttgattat ttaagatcac
    20941 caggcagtct accgttaacc acggcagcca cggagctttc cgcgattcat ggtcactcaa
    21001 catcgcaata tcgccttggc gaattttatc ttcatggtag cgacggtaaa ccactggatt
    21061 atacacaggc gagatactgg tatgagcaat cagcagaaca ggaaaatccg cgcgcacaaa
    21121 gcaaactggg gtggatctac ctgaaaggcc tgggagtcaa acccgatacc cgtaaagcaa
    21181 ttctctggta taaggaagca gctgaacaag ggtatgctca tgctcaatat actttgggtt
    21241 tgatctacag aaatggcact ggtattaatg ttaaccatta tgaatctcaa aaatggttaa
    21301 aactggccgc caaacaacat tacaaaaatg cggaaagatt acttgccgga cttcccgcac
    21361 attaacgtcg actgtcaccc gaagagatgg agtatttttc ctatcaaacc ggcaaagata
    21421 aaaatgtaga aaataatttt gatgacgccc agacagccca gcccgccatc gtcaacgggc
    21481 attggctgcg gttgcggctc cggctcgacg gtttcctgac cggtaaaatt cgccgcctga
    21541 attaataatc gacgaataat taatgcttcc agtggacaac gatcttctgc atcaaggcgg
    21601 ctcacaatcg gcattaatga ttcttctgtc ggttggctgg cctgcactaa ttgtttggca
    21661 actaaatata atggccataa gggatgttct gtatcacgcg tgagtgagat ccgcggctta
    21721 cgtcctttgt gcaaaaccat ctcgaccagt gaacgcccca gcgcagagag tttttcatcg
    21781 ctgagtattt cgtataaatg aaaacgcgta cgggcgctgt cgccggggac ataaagtagc
    21841 tgtttaccct gatcgttaac cagttcacga gcttcaatca tcatcaccac taacgggtaa
    21901 taatcgaccc gatgctgacg gcaccattgc aatattaaag catccgcctg ggtggaggga
    21961 tactcgcgcc agtagtcaca aagttctgcc agcaagcttt cgccttctcc acaatagacg
    22021 cgttgcgcgt attcataata acgcgcatct tcattatcag gttcagcttc ccgccacgac
    22081 cgggcgtaat ccagaagttc ggggatatcc aggcgacctg cgctatacca gcggagtaaa
    22141 tttctgtgca gttttgcatc atcggggatg atcactgcgc gatgttgcgc taaatacaat
    22201 gtcagccatt ctggatgcga tgaccaaatc cgagcgaaca tttggtaaaa atcgatggtg
    22261 tcattttgca cggcaacggg cagatgcaga atggtctgga agttaaaaac attccccttc
    22321 gcaatggcat ataaaaagga ttccagttct tcttcgtcta tttccccgac gttttcctcc
    22381 tgcaaccacc gcaatctttc tgctaataac accacgcact cgaaggataa atgggcagtg
    22441 ttcatggcga ttgtgcacag cgaccagcgt aattcatcaa tctcttccat cgagcaataa
    22501 tttaattgct gaatgaatcg ctgccaggcg gagggcagaa aacgttcgct atcagaggca
    22561 agtaacgcgc gaaaggcgag cagaatttca tgttctgcta cctcatattc ttctggttgc
    22621 caaacagatt tagcaggcga ctgcgcaatc cggagcgcct cctcatacgc ctgacgaagt
    22681 tgtttaaaac cctgcggatc ggtttctgga tgaaaggaca gtaacagcgc cagataagcg
    22741 cggcggataa tatcgacgtc agtcgtttcc tctatttcga ggatcttcca gcaatttttc
    22801 ataggtcgct catatcctta agcgtttccg ggcatgtggg gtagtcagcc agtggttgcc
    22861 aggacaaggc agggtaatac gcatcatcac tggcaaataa ttgataaaac atatcattgc
    22921 cggaatcgtc gtattcttta cgagctaaga attcgcgtaa taaaggcaga tgatcatctt
    22981 cgggagactg ccaatattgc cgaccaaagg tataaccggc aaagtattgc cgccagttac
    23041 tataataacg tagagcccgt agatgaatac gcgactggat ccatagactt tcttcttcac
    23101 tcaaccagtt attaagtacg cccattcgac ttaaaaaacc catccggaca taatcccatg
    23161 ctttaattcc tccctcacca cagcattcgg ctgtgctggc gacaaattgc gcataagcct
    23221 gaccttgctc atttagttca gcaagatagt cacgccattc acatggcgaa taacgaaacc
    23281 agcggtgata aaaccctgcc aaccgagcgg catgaccgtt atcgatcatt cggtgaattg
    23341 tgcgcagcag gtcatcacgg tcatcaattc cccatgagga agaaaggtca aaaaagcatg
    23401 tacctaaata cattttgggg gatgaatgcg aaggtaaaca gcattcattg cgtatttcca
    23461 tcggagtgga aagtgcaaac aatatttttt gcgaccccat gtccatataa tttatcctta
    23521 aacattcatt taatgaatgt aaactacatt tcgagactca gttatttata cttttctgac
    23581 gcaacataat acgcataaaa taaggcttac gcaaaaatgg aacacataaa aatataatat
    23641 gttttatcta caaccataag gaaatgataa tggtttttac gtcaagttgc tgcgataatt
    23701 tatcaataga tgagattatc gaacgtgctg aaaaaggcga ttgcgaggct caatatattg
    23761 ttgggtttta ttataatcgc gatagcgcaa ttgactctcc ggacgacgaa aaggcctttt
    23821 actggctgaa gctggccgct gagcaaggtc attgtgaagc acagtattcc ttagggcaaa
    23881 agtataccga ggataaaagc cgtcataaag ataatgagca agccatcttc tggctgaaaa
    23941 aagctgccct acaaggccat actttcgctt ccaacgccct tggctggaca ctggatcgtg
    24001 gagaagcccc caattataaa gaagcggttg tctggtatca gatagccgcg gagagcggaa
    24061 tgtcttatgc gcaaaataat cttgggtgga tgtacagaaa tggcaacgga gtcgcaaaag
    24121 actatgcgct ggcatttttc tggtacaaac aagctgcatt acaaggccat agtgacgcgc
    24181 aaaacaatct ggccgatctt tatgaagacg gaaaaggcgt tgctcaaaac aagacactcg
    24241 ccgcattctg gtatttgaaa agcgcacagc agggtaatcg gcacgcccag tttcaaattg
    24301 catgggatta taacgctggc gaaggggtgg accaggacta taagcaagcg atgtactggt
    24361 atctgaaggc tgccgctcag gaaagcgtcg gcgcttacgt caacatcggt tatatgtata
    24421 aacacggaca aggcgttgag aaagattatc aggctgcctt tgaatggttt acgaaagccg
    24481 ctgaatgcaa tgacgccact gcctggtata acctggccat tattatcact acggagaagg
    24541 aagacctgtc gatctccgac aggctctcga cctgtatcgt aaagttcagt catccggaac
    24601 cagagatgtc agtcaggaaa ttcgtgagac tgaagattta ctgtagagat aaaaacaagg
    24661 gccggggaat ttcattcggc ccatattctc tttattgctg tgcgaaatca gactattacg
    24721 gctcaaacgg gcgtctctgg aactggtcat gaccacattt cgggcatagc gtcagcactt
    24781 ccggtgtgta gatcgggaga tggaagtgac atttctcgca gaccagattt cccagcccga
    24841 ccacttctcc gctgtgataa accccatgat gattgaggtc ctggaaaact tcgcgccatt
    24901 caagctgcgt tttatcggtg atatctgcca gctcctgcca taagctttct ttaatcaccc
    24961 gcataaagac gctgtcagat tcttctttca ggctctcttc atagctcatg gcgaactctt
    25021 ccaggtcacg tcttacagct cgcgtcagct catcgacctc ggttcgcgtt aactcccctg
    25081 tttttattac gcgctcgcgc gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc
    25141 gcaggcgttc gctcagtgac gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg
    25201 tttcatctcc cgggtcgtta actgactata ataatagacg ttctttgttc cttgctttgc
    25261 taatacgccc acaaactctg taaattaatg tatgaataat ccgggcatca tttcaacgtc
    25321 gagtgcgcga aaggctgttt tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa
    25381 ccacctcaac gctacatttg tagccgtatt gaaaacagga ccactggctg ccatgcaaga
    25441 gcaataccgc ccggaagaga tagaatccaa agtacagctt cattgggatg agaagcgcac
    25501 atttgaagta accgaagacg agagcaaaga gaagtattac tgcctgtcta tgcttcccta
    25561 tccttctggt cgactacaca tgggccacgt acgtaactac accatcggtg acgtgatcgc
    25621 ccgctaccag cgtatgctgg gcaaaaacgt cctgcagccg atcggctggg acgcgtttgg
    25681 tctgcctgcg gaaggcgcgg cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga
    25741 caacatcgcg tatatgaaaa accagctcaa aatgctgggc tttggttatg actggagccg
    25801 cgagctggca acctgtacgc cggaatacta ccgttgggaa cagaaattct tcaccgagct
    25861 gtataaaaaa ggcctggtat ataagaagac ttctgcggtc aactggtgcc cgaacgacca
    25921 gaccgtactg gcgaacgaac aagttatcga cggctgctgc tggcgctgcg ataccaaagt
    25981 tgaacgtaaa gagatcccgc agtggtttat caaaatcact gcttacgctg acgagctgct
    26041 caacgatctg gataaactgg atcactggcc agacaccgtt aaaaccatgc agcgtaactg
    26101 gatcggtcgt tccgaaggcg tggagatcac cttcaacgtt aacgactatg acaacatgct
    26161 gaccgtttac actacccgcc cggacacctt tatgggttgt acctacctgg cggtagctgc
    26221 gggtcatccg ctggcgcaga aagcggcgga aaataatcct gaactggcgg cctttattga
    26281 cgaatgccgt aacaccaaag ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt
    26341 cgatactggc tttaaagctg ttcacccatt aacgggcgaa gaaattcccg tttgggcagc
    26401 aaacttcgta ttgatggagt acggcacggg cgcagttatg gcggttccgg gtcacgacca
    26461 gcgcgactac gagtttgcct ctaaatacgg cctgaacatc aaaccagtta tcctggctgc
    26521 tgacggctct gagccagatc tttctcagca agccctgact gaaaaaggcg tgctgttcaa
    26581 ctctggcgaa ttcaacggtc ttgaccatga agcggccttc aacgccatcg ccgataaact
    26641 gactgcgatg ggcgttggcg agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc
    26701 tcgtcagcgt tactggggcg cgccgattcc gatggtgacg ctggaagacg gtaccgtaat
    26761 gccgaccccg gacgaccagc tgccggtgat cctgccggaa gatgtggtga tggacggcat
    26821 taccagcccg attaaagcag atccggagtg ggcaaaaact accgttaacg gtatgccagc
    26881 gctgcgtgaa accgacactt tcgacacctt tatggagtcc tcctggtact atgcgcgcta
    26941 cacttgccca gagtacaaag aaggtatgct ggattccgaa gcggctaact actggctgcc
    27001 ggtggatatc tacattggtg gtatcgaaca cgccattatg cacctgctct acttccgctt
    27061 cttccacaaa ctgatgcgtg atgcaggcat ggtgaactct gacgaaccag cgaaacagtt
    27121 gctgtgtcag ggtatggtgc tggcagatgc cttctactat gttggcgaaa acggcgaacg
    27181 taactgggtt tccccggttg atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa
    27241 agcgaaagat gcggcaggcc atgaactggt ttataccggc atgagcaaaa tgtccaagtc
    27301 gaagaacaac ggtatcgacc cgcaggtgat ggttgaacgt tacggcgcgg acaccgttcg
    27361 tctgtttatg atgtttgctt ctccggctga tatgactctc gaatggcagg aatccggtgt
    27421 ggaaggggct aaccgcttcc tgaaacgtgt ctggaaactg gtttacgagc acacagcaaa
    27481 aggtgatgtt gcggcactga acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg
    27541 cgatgtgcat aaaacgatcg ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa
    27601 caccgcaatt gcggcgatta tggagctgat gaacaaactg gcgaaagcac caaccgatgg
    27661 cgagcaggat cgcgctctga tgcaggaagc gctgctggcc gttgtccgta tgcttaaccc
    27721 gttcaccccg cacatctgct tcacgctgtg gcaggaactg aaaggcgaag gcgatatcga
    27781 caacgcgccg tggccggttg ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt
    27841 ggtgcaggtt aacggtaaag tccgtgccaa aatcaccgtt ctggtggacg caacggaaga
    27901 acaggttcgc gaacgtgctg gccaggaaca tctggtagca aaatatcttg atggcgttac
    27961 cgtgcgtaaa gtgatttacg taccaggtaa actcctcaat ctggtcgttg gctaagcgcg
    28021 ggaggaagcg tgcgatatct ggcaacattg ttgttatctc tggcggtgtt aatcaccgcc
    28081 gggtgtggct ggcatctgcg tgataccacg caggttcctt ccactatgaa ggtcatgatc
    28141 ctggactcag gcgatccgaa cgggccatta agccgtgcag tgcgtaacca gttacgtctg
    28201 aatggtgtcg agttgcttga taaagaaacc acgcgtaagg acgttccatc cctgcgtttg
    28261 ggtaaagtga gcatcgcgaa agataccgca tcggtattcc gtaacggtca aacagcagag
    28321 tatcagatga tcatgacggt gaatgcgacc gtgttgatcc ccggccgtga tatctacccg
    28381 attagcgcca aagtcttccg ttcgttcttt gataacccgc aaatggcgtt agcgaaagat
    28441 aacgaacaag acatgatcgt aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt
    28501 aagctgccaa gcatccgtgc tgcggatatt cgttccgacg aagaacagac gtcgaccaca
    28561 acggatactc cggcaacgcc tgcacgcgtc tccaccacgc tgggtaactg atgattcggt
    28621 tgtacccgga acaactccgc gcgcagctca atgaagggct gcgcgcggcg tatcttttac
    28681 ttggtaacga tcctctgtta ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg
    28741 cacaaggatt cgaagaacac cacacttttt ccattgatcc caacactgac tggaatgcga
    28801 tcttttcgtt atgccaggct atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt
    28861 taccagaaaa cggaccgaat gcggcgatca atgaacaact tctcacactc accggacttc
    28921 tgcatgacga cctgctgttg atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg
    28981 ccgcctggtt tactgctctt gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc
    29041 aggctcagct tccccgctgg gttactgcgc gcgcaaaaca gctcaactta gaactggatg
    29101 acgcggcaaa tcaggtgctc tgctactgtt atgaaggtaa cctgctggcg ctggctcagg
    29161 cactggagcg tttatcgctg ctctggccag acggcaaatt gacattaccg cgcgttgaac
    29221 aggcggtgaa tgatgccgcg catttcaccc cttttcattg ggttgatgct ttgttgatgg
    29281 gaaaaagtaa gcgcgcattg catattcttc agcaactgcg tctggaaggc agcgaaccgg
    29341 ttattttgtt gcgcacatta caacgtgaac tgttgttact ggttaacctg aaacgccagt
    29401 ctgcccatac gccactgcgt gcgttgtttg ataagcatcg ggtatggcag aaccgccggg
    29461 gcatgatggg cgaggcgtta aatcgcttaa gtcagacgca gttacgtcag gccgtgcaac
    29521 tcctgacacg aacggaactc accctcaaac aagattacgg tcagtcagtg tgggcagagc
    29581 tgaaagggtt atctcttctg ttgtgccata aacccctggc ggacgtattt atcgacggtt
    29641 gatatgaaat ctttacaggc tctgtttggc ggcacctttg atccggtgca ctatggtcat
    29701 ctaaaacccg tggaaacgct ggcgaatttg attggtctga cgcgggtcac aatcatccct
    29761 aataatgttc ctccgcatcg tccccagccg gaagcgaaca gcgtgcagcg taaacacatg
    29821 cttgaactgg cgattgccga caagccatta tttactcttg atgaacgcga gctaaagcgc
    29881 aatgccccct cttacactgc gcaaacactg aaagagtggc ggcaggaaca aggaccggac
    29941 gtgccgctgg cgtttattat tggtcaggat tcactgctga cctttccgac ctggtacgaa
    30001 tacgaaacga tactcgacaa tgcacatttg atcgtctgtc ggcgtccagg ttacccactt
    30061 gaaatggcgc aaccgcaata ccagcaatgg ctggaagatc atttgacaca taacccggaa
    30121 gatcttcacc ttcagcctgc cggtaaaatt tatctggctg aaacgccgtg gtttaacatc
    30181 tcggcgacca tcatccgcga acgtttgcaa aacggtgaat cgtgtgagga tttattgccg
    30241 gaaccggtac tgacttacat taaccaacaa ggcttgtatc gctgatacct gtcgttacgg
    30301 agggcatgat gcgactgtgg ttaattcgtc atggtgaaac gcaagcgaat atcgatggtc
    30361 tttacagcgg tcatgcgccc acccccctga ccgcgcgcgg tattgagcaa gcgcaaaatc
    30421 tgcatacgct gctacatggt gtttcctttg atctggtttt atgcagtgaa ctggaacggg
    30481 cacagcatac cgcacgactg gttctcagtg accgccagct ccccgtgcaa atcatccctg
    30541 aactcaacga aatgtttttt ggcgactggg agatgcgaca tcatcgcgac ctcatgcaag
    30601 aagatgccga aaactatagc gcgtggtgca atgactggca gcatgcaatc cccacgaacg
    30661 gtgaaggatt tcaggcattt tcgcaacgtg tggaacgctt tatcgcaagg cttagtgaat
    30721 ttcagcacta tcagaatatt ttagtcgtca gccatcaggg tgtactgagt ctgttaatcg
    30781 cccgtttaat tggcatgcct gccgaggcta tgtggcattt tcgcgttgac taggggtgtt
    30841 ggagcgccat tgatatcaac caaaaattcg cgacgctacg cgtcctcaat agccgtgcca
    30901 tcggggtcga gaatgcatga cttttctgtt tttttacggg taagccgcaa cgaccattga
    30961 cagacccggg caggctgata ttctccgcag ccagactttt ttcgccagac acgactttgt
    31021 agaaattgtt ttacaaaaat ggcgatgcaa tctgcggcgc ggggtgggat gatagcccac
    31081 tttcgaaagc cgattcggcg acaattgtcc cgaaatcgcc tctggttcag gtatactgac
    31141 agaccatttt tatctatttg attcacccag ggggaaaact tgcagggtaa agcactccag
    31201 gattttgtta tcgacaaaat tgatgacctc aaaggtcagg acatcatcgc cttagacgtt
    31261 caaggcaaat ccagcatcac cgactgcatg atcatctgta cgggtacgtc cagccgtcat
    31321 gttatgtcca ttgctgacca cgttgtgcag gagtctcgcg cagcgggcct gttaccgctc
    31381 ggcgtagaag gtgaaaacag cgccgactgg attgtcgtgg atttgggcga tgtgattgtc
    31441 catgtcatgc aggaagagag ccgtcgcctg tatgaactgg aaaaactctg gagttaatgc
    31501 gtgaagctgc aacttgtcgc cgtgggaacg aaaatgccgg actgggtaca aaccggtttt
    31561 accgagtacc tgcgtcgttt tccgaaagat atgcccttcg agctgattga aattccggcc
    31621 ggaaaacgcg gcaagaatgc ggacatcaag cgcatactcg acaaagaggg tgagcagatg
    31681 ttggcggccg caggcaaaaa ccgcattgtc accctcgata ttccaggcaa gccctgggat
    31741 acgccgcagt tagccgctga gctggaacgc tggaagctgg atggtcgcga cgtcagtcta
    31801 ctgattggcg ggcctgaagg gttgtcgcct gcctgtaaag cggcggcgga gcagagctgg
    31861 tcgctgtcgg cgcttaccct cccccatccg ctggttcgcg tgctggtcgc agagagtctg
    31921 taccgggcgt ggagcatcac caccaaccat ccttatcacc gtgagtgata agggagcttt
    31981 gagtagaaaa cgcagcggat gaaactacag aactcttttc gcgactatac ggcagagtcc
    32041 gcgctgtttg tgcgccgggc gctggtcgcc tttttgggga ttttgctgct gaccggcgtg
    32101 cttatcgcca acctgtataa tctgcaaatt gttcgcttta ccgactacca gactcgctct
    32161 aatgaaaacc gcattaagct ggtgcctatc gcgcccagcc gcggcattat ctacgaccgt
    32221 aatggtatcc ctctggccct caaccgcact atctaccaga tagaaatgat gccagagaaa
    32281 gtcgataacg tgcagcaaac gctggacgct ttgcgcagcg tggttgatct gaccgatgac
    32341 gatattgctg cattccgaaa agagcgcgca cgttcacacc gtttcacctc tattccggtg
    32401 aaaactaacc tgaccgaagt acaagtagct cgctttgccg tcaatcagta ccgttttccg
    32461 ggtgtcgaag ttaaaggcta taaacgtcgt tactatcctt acggttcggc gttgacccac
    32521 gtcatcggct atgtgtcgaa aatcaacgat aaagacgtcg aacgcctgaa taatgacggc
    32581 aaactggcca actatgcggc aacgcatgat atcggtaagc tgggcattga gcgttactat
    32641 gaagacgtgc tgcacggtca gaccggttat gaagaggttg aagttaacaa ccgtgggcgt
    32701 gttatccgcc agttaaaaga agtaccaccg caagccggac acgatattta cctgacgctg
    32761 gatctcaaac tccagcaata tattgaaacg ctgctggcgg gtagccgcgc agctgtggtt
    32821 gtcaccgatc cgcgtacagg tggggtgctg gcgctggttt ccacgcctag ttatgaccca
    32881 aacctgtttg ttgacggtat ctccagcaaa gattattccg ccttgttgaa cgccccgaat
    32941 acaccgctgg tgaaccgcgc cacacagggg gtttatcctc ccgcgtctac agttaaaccc
    33001 tatgtggcgg tttcggcatt gagcgccggg gtgatcacgc gcaatacgac gctgtttgac
    33061 ccaggctggt ggcaactgcc aggttcggaa aaacgttatc gtgactggaa aaaatggggc
    33121 cacgggcgtc tgaatgtcac aagatcgctg gaagaatctg cggatacctt cttctatcag
    33181 gtggcctacg atatggggat cgatcgcctc tccgaatgga tgggtaaatt cggttatggt
    33241 cattacaccg gtatcgacct ggcggaagaa cgttccggca acatgcctac ccgcgaatgg
    33301 aaacagaaac gctttaaaaa accgtggtat cagggtgaca ccattccggt tggtatcggt
    33361 cagggttact ggacagcgac cccaatccag atgagtaagg cactgatgat cctgattaat
    33421 gacggtatcg tgaaggttcc tcatttgctg atgagcaccg ccgaagacgg caaacaggtg
    33481 ccatgggtac agccgcatga accgcccgtc ggcgatattc attccggtta ctgggagctg
    33541 gcgaaagacg gtatgtacgg tgttgctaac cgccctaacg gtacggcgca taaatacttt
    33601 gccagcgcac cgtacaaaat tgcggcgaaa tccggtaccg ctcaggtctt cggtctgaaa
    33661 gcgaacgaaa cctataatgc gcacaaaatt gccgagcgtt tacgtgacca caaactgatg
    33721 accgcctttg cgccatacaa caatccgcaa gtggctgtcg ccatgattct ggagaacggt
    33781 ggtgcgggtc cggcggttgg tacactgatg cgccagatcc tcgaccacat tatgctgggt
    33841 gataacaaca ccgatctgcc tgcggaaaat ccagcggttg ccgcagcgga ggaccattaa
    33901 tcatgacgga taatccgaat aaaaaaacat tctgggataa agtccatctc gatcccacaa
    33961 tgctgctgat cttactggca ttgctggttt acagcgccct ggttatctgg agcgccagcg
    34021 gtcaggatat tggcatgatg gagcgtaaaa tcggccaaat cgcgatgggt ctagtcatca
    34081 tggtggtgat ggcgcaaatt cctccacgcg tttatgaagg ctgggccccc tatctctata
    34141 tcatctgtat tattttgctg gtggcggtag atgctttcgg tgccatctct aaaggtgctc
    34201 aacgctggct ggacctcggt attgttcgtt ttcagccgtc ggaaattgcc aaaatagccg
    34261 taccactgat ggttgcgcgc tttatcaacc gcgacgtttg cccgccatcg ttgaagaaca
    34321 ctggcatcgc gctggtgctg atatttatgc ccacgctgct ggtggctgca cagcctgacc
    34381 tgggaacatc aatcctcgtt gcgctttccg gtctgtttgt actgttcctc tctggcctta
    34441 gctggcgtct gattggcgtc gcagtagtgc tggtggcggc gttcattccg attctgtggt
    34501 tcttcctgat gcatgattac cagcgccagc gcgtaatgat gctcctagac ccggaatcag
    34561 acccactcgg cgcgggctat cacattattc agtctaaaat tgctattggc tccggcggat
    34621 tacgcggcaa aggctggctg cacggcactc agtcacagct tgaatttctc cccgaacgcc
    34681 ataccgactt tatcttcgcg gtactggcgg aagagctggg attagtgggc attctgattc
    34741 tgctcgctct ctacattctg ctgatcatgc gcgggctgtg gatagccgcc agagcgcaaa
    34801 ccacctttgg tcgcgtcatg gctggtggct taatgctgat attattcgtt tatgtcttcg
    34861 taaatattgg tatggtaagc ggtattctgc cggttgtagg ggttccgctc ccactggtca
    34921 gttatggagg atcggcgcta attgtgctga tggctgggtt cgggattgta atgtcaatcc
    34981 acacccacag gaaaatgttg tcgaaaagcg tgtaagaggt gcgcaatgcg taagcagtgg
    35041 ctcgggatct gcatcgcggc aggaatgctc gcggcatgta caagcgatga tggtcagcaa
    35101 cagacggtaa gtgtaccgca gcctgcggta tgtaacggcc ccatagttga aattagcggg
    35161 gcggacccgc gtttcgaacc actgaacgcg acggcaaatc aggattacca gcgcgacggt
    35221 aaaagctaca aaatcgtgca ggatccgtct cgatttagcc aggcgggact ggcggcaatc
    35281 tatgatgccg aacccggcag taacctgacg gcctctggcg aagctttcga tccgacacag
    35341 ctgacggcgg cccatccaac gcttccgatc cccagctacg ccagaatcac taacctggct
    35401 aacgggcgaa tgatcgtggt gcgcattaat gatcgcggcc cttacggcaa cgaccgcgtt
    35461 atttcacttt ctcgcgcggc agctgaccgt cttaacacgt caaacaacac caaagttcgt
    35521 atcgatccga ttattgtcgc ccaggatggt tcgctttctg gtcctggtat ggcttgtacc
    35581 acagtcgcca aacagactta cgccctgcca gcacctcccg atttaagcgg tggcgcggga
    35641 acaagttcag tgtctggccc gcagggtgac attcttccgg tcagtaattc gacgctaaaa
    35701 agcgaagatc cgaccggcgc gccggtaacc agtagcggtt tcctcggcgc tccaacgacc
    35761 ttagcgcctg gtgtactgga aggcagcgaa ccgacgcctg cgccacagcc cgttgttaca
    35821 gctccgtcga caacgcctgc aacctcgcct gcaatggtga caccgcaagc cgcctcgcaa
    35881 agcgccagcg gcaactttat ggtgcaagtc ggggccgtaa gcgatcaggc tcgtgcgcaa
    35941 cagtaccaac agcaactggg acagaagttc ggcgtccccg gtcgcgtaac tcaaaatggc
    36001 gcggtctggc ggatccagct tggcccattc gccagcaaag ccgaagccag taccttgcag
    36061 caacgtttgc aaaccgaagc ccaattacag tcatttatta ctaccgcgca gtagcgtaaa
    36121 gcaggcatct gatgtgtcaa tttctgtaaa tgacgttaac aaagtcacgc ggaaagtcag
    36181 atgcctgccg gtagtgcatt tgctatagta gggcactttt ttaattccat cacggatgtc
    36241 gtagttcaga ccatgaatac cattttttcc gctcgtatca tgaagcgcct ggcgctcacc
    36301 acggctcttt gcacagcctt tatctctgct gcacatgccg atgacctgaa tatcaaaact
    36361 atgatcccgg gtgtaccgca gatcgatgcg gagtcctaca tcctgattga ctataactcc
    36421 ggcaaagtgc tcgccgaaca gaacgcagat gtccgccgcg atcctgccag cctgaccaaa
    36481 atgatgacca gttacgttat cggccaggca atgaaagccg gtaaatttaa agaaactgat
    36541 ttagtcacta tcggcaacga cgcatgggcc accggtaacc cggtgtttaa aggttcttcg
    36601 ctgatgttcc tcaaaccggg catgcaggtt ccggtttctc agctgatccg cggtattaac
    36661 ctgcaatcgg gtaacgatgc ttgtgtcgcc atggccgatt ttgccgctgg tagccaggac
    36721 gcttttgttg gcttgatgaa cagctacgtt aacgcactgg gcctgaaaaa tacccacttc
    36781 cagacggtac atggtctgga tgctgatggt cagtacagct ccgcgcgaga tatggcgctg
    36841 atcggccagg cattgatccg tgacgtaccg aatgaatact cgatctataa agaaaaagaa
    36901 tttacgttta acggtattcg ccagctgaac cgtaacggcc tgttatggga taacagcctg
    36961 aatgtcgacg gcatcaaaac cggacacact gacaaagcag gttacaacct tgttgcttct
    37021 gcgactgaag gccagatgcg cttgatttct gcggtgatgg gcggacgtac ttttaaaggc
    37081 cgtgaagccg aaagtaaaaa actgctaacc tggggcttcc gtttcttcga aaccgttaac
    37141 ccactgaaag taggtaaaga gttcgcctct gaaccggttt ggtttggtga ttctgatcgc
    37201 gcttcgttag gggttgataa agacgtgtac ctgaccattc cacgtggtcg catgaaagat
    37261 ctgaaagcca gctatgtgct gaacagcagt gaattgcatg cgccgctgca aaagaatcag
    37321 gtcgtcggaa ctatcaactt ccagcttgat ggcaaaacga tcgagcaacg cccgctggtt
    37381 gtgttgcaag aaatcccgga aggtaacttc ttcggcaaaa tcattgatta cattaaatta
    37441 atgttccatc actggtttgg ttaaaaattg aacacttgaa agtgtaattt ccgtccccat
    37501 atactaagca tcagtaaaaa actcccgcct tctggcggga gttgctattt aattacgtta
    37561 cgccggagct gacatgaaaa ccaaacttaa cgaactgctt gaattcccta ctccttttac
    37621 ttacaaagtt atggggcagg cgttacctga gctggttgat caggtggttg aagtggtaca
    37681 gcgccatgcg ccaggtgact acaccccaac ggtaaaacca agcagcaaag gcaactacca
    37741 ctcggtatct atcactatca acgccactca tatcgagcag gttgaaacac tgtatgaaga
    37801 actaggcaaa atcgatattg tccgcatggt tctgtaactc gcttctccgt tacccggccc
    37861 ctggtcgggt aactcccctc tcctcgctgt gatatacttt tcccccactt ttactcattc
    37921 tccacggaga tgcagttttg tatcaggata aaattcttgt ccgccagctc ggtcttcagc
    37981 cttacgagcc aatctcccag gctatgcatg aattcaccga tacccgcgat gatagtaccc
    38041 ttgatgaaat ctggctggtc gagcactatc cggtattcac ccaaggtcag gcaggaaaag
    38101 cggagcacat tttaatgccg ggtgatattc cggtgatcca gagcgatcgc ggtgggcagg
    38161 tgacttatca cgggccgggg caacaggtga tgtatgtgtt gcttaacctg aaacgccgta
    38221 aactcggtgt gcgtgaactg gtgaccttgc ttgagcaaac agtggtgaat accctggctg
    38281 aactgggtat agaagcgcat cctcgggctg acgcgcctgg tgtctatgtc ggggaaaaga
    38341 aaatttgctc actgggttta cgtattcgac gcggttgttc attccacggt ctggcattaa
    38401 acgtcaatat ggatctttca ccatttttac gtattaatcc ttgtgggtat gccggaatgg
    38461 aaatggctaa aatatcacaa tggaaacccg aagcgacgac taataatatt gcaccacgtt
    38521 tactggaaaa tattttagcg ctactaaaca atcctgactt cgaatatatt accgcttaat
    38581 tgcatacctc aatggcccaa tttacactgg gtcattactc ataatacctg ctaatgcttt
    38641 acatctcctt cgcgtttgta tactttcttt atcacttgca gaaatgcggt gctacgcctt
    38701 gctccgtaac tcaccccatt gattaaccta ttgaaatagt tgattatggt gagcaaaagc
    38761 gttccgttcc aattagacat aagattattg taattttttt aaatattatt tttccatgaa
    38821 taaaattgga gtaagtgcgt ggatagtaat aatcaaattg aaccctgttt aagcagaaag
    38881 tcgtcagaag gtaaaccaca aatatttaca accttacgaa atattgatct taaccttctg
    38941 actatttttg aagctgtata tgtacataaa gggatcgtta atgcagcgaa agtgcttaat
    39001 ctgaccccct cggcaatcag tcagtctatt cagaaactgc gcgttatatt ccctgaccca
    39061 ttgtttattc gcaaaggcca gggtgtcact cctaccgcat ttgcgatgca tctacatgag
    39121 tatatcagtc agggccttga gtccattctt ggcgcgctgg atatcgaagg aagctatgat
    39181 aagcaacgaa cgataactat tgcgaccact ccctcggtcg gagccctggt ccttcctgtc
    39241 atctatcggg cgattaaaac tcactatccg cagcttttac tgcgcaaccc gcccatcagc
    39301 gacgcggaaa accaactcag tcagtttcaa accgatctca tcatcgataa catgttttgc
    39361 accaatcgta cggtgcaaca tcatgttctg ttcaccgaca atatggtgtt aatttgccgc
    39421 gagggaaatc cactactctc tttagaagat gacagagaga ctatcgacaa cgctgcgcat
    39481 gtactcctgt taccggaagg gcaaaatttc agcggtctgc ggcagagagt tcaagagatg
    39541 tttccggacc ggcaaatcaa tttcaccagc tacaacattt tgacaatcgc tgcactggtt
    39601 gccaacagtg acatgttagc gattattccc agccgttttt ataacctgtt tagtcgttgc
    39661 tggccgctgg aaaaactgcc ttttccgtcc ttaaatgagg agcaaataga tttctctatc
    39721 cactacaaca aattcagcct gcgtgatccc atcctgcacg gggtaataga tgttatccgt
    39781 aatgcatttt gaaaaagtat tgttacagcc agatcacagc gcagaacgat aaaatggcac
    39841 aatccaacaa caattttaca ttttcgcgac cgctttggct gctttcaggt ccgtttcaat
    39901 gatatactgc cagtcgttaa ttcaaaaata gttgataatt acaacaatct attgaattga
    39961 aacgctttcc ttcgtaattc gcaactggaa cacgcacgct atgagtaaac ccattgtgat
    40021 ggaacgcggt gttaaatacc gcgatgccga taagatggcc cttatcccgg ttaaaaacgt
    40081 ggcaacagag cgcgaagccc tgctgcgcaa gccggaatgg atgaaaatca agcttccagc
    40141 ggactctaca cgtatccagg gcatcaaagc cgcaatgcgc aaaaatggcc tgcattctgt
    40201 ctgcgaggaa gcctcctgcc ctaacctggc ggaatgcttc aaccacggca cagcaacgtt
    40261 tatgatcctc ggcgctattt gtacccgccg ttgtccgttc tgtgatgttg cccacggtcg
    40321 cccggtagct cctgatgcca atgaaccagt gaaactggcg cagaccattg ccgatatggc
    40381 gctgcgttat gtggttatca cctccgttga ccgtgatgac ctgcgcgatg gcggtgccca
    40441 gcactttgcg gattgcatta ctgccattcg ggaaaaaagc ccgcaaatca aaattgaaac
    40501 tctggtgccg gatttccgtg gtcgtatgga tcgtgctctg gatattctga ctgcaacgcc
    40561 accagatgtg ttcaaccata acctggaaaa cgtaccgcgt atttaccgtc aggtacggcc
    40621 tggtgcagat tacaactggt cgctgaagct gctggaacgc tttaaagaag cgcatccgga
    40681 aatcccgacc aagtctggtc tgatggtggg actgggtgaa accaatgaag aaattattga
    40741 ggtaatgcgc gacctgcgcc gtcatggtgt gacgatgtta acgctggggc aatatttgca
    40801 gccaagccgc catcacctgc cggttcaacg ttacgttagc ccggatgagt tcgacgaaat
    40861 gaaagccgaa gcgctggcga tgggctttac ccatgctgca tgcggtccgt ttgtccgctc
    40921 ttcttaccac gccgatttgc aggcgaaagg gatggaagtt aagtaatcct gtaataatta
    40981 cttacatctg tctgataaaa aacccgcttt ttg
//
LOCUS       DAETQN010000021        39837 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.021, whole genome shotgun sequence.
ACCESSION   DAETQN010000021 DAETQN010000000
VERSION     DAETQN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.021"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            4..435
                     /locus_tag="K0W68_001176"
     CDS             4..435
                     /locus_tag="K0W68_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBI3072793.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQFA
                     VQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="K0W68_001177"
     CDS             518..1420
                     /locus_tag="K0W68_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HBI3072794.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="K0W68_001178"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="K0W68_001178"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBI3072795.1"
                     /translation="MDVSYLLDSLNDKQRETVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="K0W68_001179"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="K0W68_001179"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBI3072796.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="K0W68_001180"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="K0W68_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBI3072797.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="K0W68_001181"
     CDS             complement(5330..6037)
                     /locus_tag="K0W68_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBI3072798.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="K0W68_001182"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="K0W68_001182"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBI3072799.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="K0W68_001183"
     CDS             complement(6895..7098)
                     /locus_tag="K0W68_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI3072800.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            7659..7979
                     /gene="cyaY"
                     /locus_tag="K0W68_001184"
     CDS             7659..7979
                     /gene="cyaY"
                     /locus_tag="K0W68_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBI3072801.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8019..10565)
                     /gene="cyaA"
                     /locus_tag="K0W68_001185"
     CDS             complement(8019..10565)
                     /gene="cyaA"
                     /locus_tag="K0W68_001185"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBI3072802.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10952..11893
                     /gene="hemC"
                     /locus_tag="K0W68_001186"
     CDS             10952..11893
                     /gene="hemC"
                     /locus_tag="K0W68_001186"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBI3072803.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11890..12630
                     /gene="hemD"
                     /locus_tag="K0W68_001187"
     CDS             11890..12630
                     /gene="hemD"
                     /locus_tag="K0W68_001187"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBI3072804.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            12652..13863
                     /gene="hemX"
                     /locus_tag="K0W68_001188"
     CDS             12652..13863
                     /gene="hemX"
                     /locus_tag="K0W68_001188"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBI3072805.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            13866..15062
                     /gene="hemY"
                     /locus_tag="K0W68_001189"
     CDS             13866..15062
                     /gene="hemY"
                     /locus_tag="K0W68_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBI3072806.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15724..15885
                     /locus_tag="K0W68_001190"
     CDS             15724..15885
                     /locus_tag="K0W68_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072807.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15969..17204)
                     /locus_tag="K0W68_001191"
     CDS             complement(15969..17204)
                     /locus_tag="K0W68_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBI3072808.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17351..17427)
                     /locus_tag="K0W68_001192"
     tRNA            complement(17351..17427)
                     /locus_tag="K0W68_001192"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17391..17393),aa:Pro,seq:tgg)
     gene            complement(17470..17556)
                     /locus_tag="K0W68_001193"
     tRNA            complement(17470..17556)
                     /locus_tag="K0W68_001193"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17520..17522),aa:Leu,seq:cag)
     gene            complement(17577..17652)
                     /locus_tag="K0W68_001194"
     tRNA            complement(17577..17652)
                     /locus_tag="K0W68_001194"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17617..17619),aa:His,seq:gtg)
     gene            complement(17711..17787)
                     /locus_tag="K0W68_001195"
     tRNA            complement(17711..17787)
                     /locus_tag="K0W68_001195"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17751..17753),aa:Arg,seq:ccg)
     gene            complement(17888..19273)
                     /locus_tag="K0W68_001196"
     CDS             complement(17888..19273)
                     /locus_tag="K0W68_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI3072809.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19464..20204)
                     /gene="rffM"
                     /locus_tag="K0W68_001197"
     CDS             complement(19464..20204)
                     /gene="rffM"
                     /locus_tag="K0W68_001197"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBI3072810.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20207..21559)
                     /gene="wzyE"
                     /locus_tag="K0W68_001198"
     CDS             complement(20207..21559)
                     /gene="wzyE"
                     /locus_tag="K0W68_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HBI3072811.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21556..22635)
                     /gene="rffT"
                     /locus_tag="K0W68_001199"
                     /gene_synonym="wecF"
     CDS             complement(21556..22635)
                     /gene="rffT"
                     /locus_tag="K0W68_001199"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBI3072812.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(22632..23882)
                     /gene="wzxE"
                     /locus_tag="K0W68_001200"
     CDS             complement(22632..23882)
                     /gene="wzxE"
                     /locus_tag="K0W68_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBI3072813.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23884..25014)
                     /gene="rffA"
                     /locus_tag="K0W68_001201"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23884..25014)
                     /gene="rffA"
                     /locus_tag="K0W68_001201"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBI3072814.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(25019..25693)
                     /gene="rffC"
                     /locus_tag="K0W68_001202"
                     /gene_synonym="wecD"
     CDS             complement(25019..25693)
                     /gene="rffC"
                     /locus_tag="K0W68_001202"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBI3072815.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25671..26552)
                     /gene="rfbA"
                     /locus_tag="K0W68_001203"
     CDS             complement(25671..26552)
                     /gene="rfbA"
                     /locus_tag="K0W68_001203"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBI3072816.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26571..27638)
                     /gene="rffG"
                     /locus_tag="K0W68_001204"
     CDS             complement(26571..27638)
                     /gene="rffG"
                     /locus_tag="K0W68_001204"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBI3072817.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27638..28900)
                     /gene="wecC"
                     /locus_tag="K0W68_001205"
     CDS             complement(27638..28900)
                     /gene="wecC"
                     /locus_tag="K0W68_001205"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBI3072818.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28897..30027)
                     /gene="wecB"
                     /locus_tag="K0W68_001206"
     CDS             complement(28897..30027)
                     /gene="wecB"
                     /locus_tag="K0W68_001206"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBI3072819.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(30083..31129)
                     /gene="wzzE"
                     /locus_tag="K0W68_001207"
     CDS             complement(30083..31129)
                     /gene="wzzE"
                     /locus_tag="K0W68_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBI3072820.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31141..32244)
                     /gene="wecA"
                     /locus_tag="K0W68_001208"
     CDS             complement(31141..32244)
                     /gene="wecA"
                     /locus_tag="K0W68_001208"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBI3072821.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32484..33743)
                     /gene="rho"
                     /locus_tag="K0W68_001209"
     CDS             complement(32484..33743)
                     /gene="rho"
                     /locus_tag="K0W68_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBI3072822.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33828..33929)
                     /gene="rhoL"
                     /locus_tag="K0W68_001210"
     CDS             complement(33828..33929)
                     /gene="rhoL"
                     /locus_tag="K0W68_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBI3072823.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(34070..34399)
                     /gene="trxA"
                     /locus_tag="K0W68_001211"
     CDS             complement(34070..34399)
                     /gene="trxA"
                     /locus_tag="K0W68_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBI3072824.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34530..35795
                     /gene="rhlB"
                     /locus_tag="K0W68_001212"
     CDS             34530..35795
                     /gene="rhlB"
                     /locus_tag="K0W68_001212"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBI3072825.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35929..37413
                     /gene="gppA"
                     /locus_tag="K0W68_001213"
     CDS             35929..37413
                     /gene="gppA"
                     /locus_tag="K0W68_001213"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBI3072826.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37460..39481)
                     /gene="rep"
                     /locus_tag="K0W68_001214"
     CDS             complement(37460..39481)
                     /gene="rep"
                     /locus_tag="K0W68_001214"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBI3072827.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9866 a        10832 c        10049 g         9090 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca agttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgtt tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 atgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggccgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg ctttctgctt
     7141 tctgctttct gctttctgct ttctgctttc tgctttctgc tttctgcttt ctgctttctg
     7201 ctttctgctt tctgctttct gctttctgct ttctatcatc gcaggagaag acgtaaaagc
     7261 aagcgtttag cgactcttta tgaagtctga aaagtgagga aatgctgcga gtataacgct
     7321 tactcgtggg gatttatgtt ttgctggtag tttcgtgatc ggcacctcgc gccaggatgg
     7381 ctctatgcac tatcgcaatg tggtttgttt gttgtctttt ttagttccgt cagcgtgagg
     7441 atgctcgctc ggtaaacctg atcataatgt ttaccagaaa cagggaaagg cgtggtgtaa
     7501 ctgcgcccta atgaagagac aaatctttcg ctacacagag acatcattat tccggtgacg
     7561 gatgaatcgt gccggaacgc agccgcagag gtgcaaactt tgaagccctg accccatgcc
     7621 gctatactgc cgccatcact taaaaacagg atacaacaat gaacgacagt gaatttcatc
     7681 gcctggctga tcaactctgg ctgaccattg aagaacgcct ggacgactgg gatggcgaca
     7741 gcgatatcga ctgcgaaatc aacggcggcg tgctgaccat tacctttgaa aacggcagca
     7801 aaatcattat caaccgccag gaaccgctgc accaggtatg gctggcaacc aaacaaggcg
     7861 gctaccattt tgatctgaaa ggcgatgagt ggatttgcga tcgcagcggc gaaaccttct
     7921 gggatttgct ggaacaggcg gcgacgcagc aggcgggtga aacagtcagt ttccgctaag
     7981 attgcatgcc ggataagcct cgctttccgg cacgttcatc acgaaaaata ttgctgtaat
     8041 agcggcgtat cgtgatcctg attggcaggc ggcatgttac cgatagattt tgtgcggaac
     8101 ggaatcacct gttcacgacc atcaaccttc acaatctgat agaactgcgg caggttgaag
     8161 ttgatgaagc ttgagccgta ggtgaagcgg tcgtgcgatg acgagtagaa gcgactgacg
     8221 tcacgcacca gttcctcttt gctgccttcg cagtggtgat acacctcaac ccggttgctt
     8281 tcgtcaagaa tgtagatatt aaagccattc tcgtcttgcg tttcttcgaa gaagaactgg
     8341 atgatccctt cactagcaaa gccgtccacc accgccggta atttgacgtg attggtttca
     8401 acctgcactg acaggccgtg cagtttgtta tgcgaaatag cgccataaaa ctcgatggcg
     8461 ttctccagct tctgtaccga tacattcagg cgttcgaaga acaagcccca ggtttgacca
     8521 gaaacgcgca gcgccttgaa acgcccggtt tcctggcggg tgctggaaag gcgcagttca
     8581 atacactcag aaaccagttg ctgcacgcga gtacggatta acccgcgcag atgctggcta
     8641 taacagaaga cttccacgct atctggcggt gcggcgtcct gatgcatttt gccgagaata
     8701 gttttcaggg cttcgatcat cgattgctcg ccgttgaagt gcagcgtacg cacttcgttc
     8761 cacgagttgc ggtacagcag gtcaacgcta cctaccaggc aattttgatt ctcgccaaag
     8821 ctgaagacat ccagcttacg gaaatcaaaa tgcaccacct gattgcggaa cgccgctgtc
     8881 gggtcatatt ccaggttaac gataatcgcc agatggcgga tctcacacgg gctgtagagc
     8941 gccttcggtg tcggtgcagg taagcgcagc gggaaatggt gcgacacgtc ggcgaccatc
     9001 tcctgcaact taggcaaatc gacaatgccg ttacctttaa tatacaaacg ggtgcgcgag
     9061 gtcagcagac cgttaaacca tgcccacgcc accagtttat tcaggtaacg gttatattcc
     9121 agcggctgat ggctgatgat cgactcaata tttggcgcgc ggttatacag ataccaacct
     9181 gaacggttag cccggcccgg cggcacataa ataaaggtca gattcggttc cgagagatcg
     9241 ggtgaaatct gcgggtttac cagcgtcact ttacctggta atgcttcaaa cgcggcatac
     9301 agcttacgcg tcagcacgcc gatatcctgc ggactggcgg agacgctaag gttattgcga
     9361 cgcgcaaagc ggatcagatt acggtagctc tgcatcatcg cgtcgagcaa ctcgttgtgc
     9421 gcctcacgca cctgatcaat cttccagtta gcacggttat cgagcattgc cagacgagct
     9481 tcgtcccaac cccactcgct cactaactgg ctcaacactg cgcgacgcca gcctacacag
     9541 gcgcgttcac ggctgagttt ttcgcacact tttaaataga agcagcgacg tactaaatcc
     9601 agacgggtga aatcttcaat cgccgtcagg tattcagtaa cacgctccag catcatgcag
     9661 tatggatcga ggccaaacga tacaatctcg ccgtcgtgca aacgctgttt gatatctttc
     9721 gccagcagac gtgggttcgg gtattcccag gaataggctt ccagcagcag tgttttcagc
     9781 accgctttgt atggggaatc gatactcttg tagagctgcc aaaggctggc accaaagtac
     9841 tcttcagcag aaagcgagct taagccaccg agatccagcc attcatttgg cgtcagcacg
     9901 ccctgcgcgt aaagcgtcat cacatagtcg tcgtaatgct cttcttcgtc gcacggcacc
     9961 atattccaca gaatacgctt accggcgaga cgcacggcgg tacgataaaa ttcgtcaagc
    10021 agcagtatat gctgggtgga gccacaatct tcgcccccca ggctgccgct ttcattatga
    10081 cgaaagcggt tttcatcaat caggaagaag ctgacttcca cacccagcga ggcggcccag
    10141 ctttccagca ggctgcattt acgttgtagt aactggcgct cttcgctatc gagccaggat
    10201 tgatgacaga cccagatatc caggtcagag gaacaacttt gccctacgga cgaggtgctg
    10261 cccatggtgt atacaccagt aattggaagc tcacctttcg gtggatcctg tactgacatt
    10321 ccacgataca gttcaagctc gttcaggtag tggcgttgag tttcatcagg cgtgtaaagg
    10381 caaatgcctt tgggaacgtt accatcaagg taacccggca ttagcggatg gtgatagtgc
    10441 aacaatgtcg gcagtagact gtagacctgt tggaatgcag gccccatagc agcaagcgcg
    10501 cgatccacac gcaattgatt tatggcatcc agtctctgtt tcagagtctc aatatagagg
    10561 tacaagacgt atcgcctgat ttgctacccg tcatgactgt gattccgcca acatcaacgg
    10621 taacacgcgg cattcgggat atttcgtatg tcaaaggtaa ccgttaccac ttttcgcgcc
    10681 tggttttttt agtttcacga cgaaaaaatg gtctaaaacg tgatcaattt aacaccttgc
    10741 tgattgaccg taaagaaaga tgcgctacat acaagtgtag caccgtttat tctctgtaaa
    10801 ttccttatta cgacagcgtg aaacgtctgt caggatccac tgccagacct cattttacgg
    10861 tttgcgcagg cgtctacgtt tcaccacaac actgacatca ctctggcaag gatgttagga
    10921 tggaccacgg atgataatga cggtaacaag catgttagac aatgttttaa gaattgccac
    10981 acgccaaagc ccacttgcac tctggcaggc acactatgtc aaagacaagt tgatggcgag
    11041 ccatccgggc ctggtcgttg aactggtacc gatggtgacg cgcggcgatg tgattcttga
    11101 tacgccgctg gcgaaagtag gcggaaaagg cttatttgta aaagagctgg aagtcgcgct
    11161 cctcgaaaat cgcgccgata tcgccgtaca ttcaatgaaa gatgtgccgg ttgaattccc
    11221 gcaaggtctg ggactggtca ctatttgtga gcgtgaagat cctcgcgatg cctttgtgtc
    11281 caataactat gacagtctgg atgcgttacc agcaggcagt atcgtcggga cgtccagttt
    11341 acgtcgccag tgccaactgg ctgaacgccg cccggatctg attatccgct ccctgcgcgg
    11401 caacgtcggc actcgcctga gcaaactaga taacggcgaa tacgatgcca tcattcttgc
    11461 cgtagccgga ctaaaacgtt taggtctgga gtcccgcatt cgcgccgcgt taccacccga
    11521 gatttctctt ccggcggtag gacaaggtgc agtcggtatt gaatgccgcc ttgatgatac
    11581 gcgcactcgc gagctgcttg ccgcgctgaa tcaccacgaa actgcactgc gcgttaccgc
    11641 agaacgcgcc atgaataccc gtctcgaagg cggatgtcag gtgccaattg gtagctacgc
    11701 cgagcttatt gatggcgaaa tctggctgcg tgcgctggtc ggcgcgccgg acggttcgca
    11761 gattattcgc ggtgaacgcc gcggtgcgcc gcaaaatgcc aaacaaatgg ggatttcgct
    11821 ggcagaagag ctactgaata acggcgcgcg cgagatcctc gctgaagtct ataacggaga
    11881 cgccccggca tgagtatcct tgtcacccgc ccgtctcccg ctggagaaga gttagtgagc
    11941 cgtctgcgca cactggggca ggtggcctgg cattttccgc tgattgagtt ttctccgggt
    12001 caacaattac cgcaacttgc tgatcaactg gcagcgctgg gggagagcga tctgttgttt
    12061 gccctctcgc aacacgcggt tgcttttgcc caatcacagc tacatcagca agatcgtaaa
    12121 tggccccgac tacctaatta tttcgccatt ggacgcacca ccgcactggc actacatacc
    12181 gtaagcggac agaagattct ctacccgcag gatcgggaaa tcagcgaagt cttgctacaa
    12241 ttacctgaat tacaaaatat tgcgggcaaa cgtgcgctga tattacgtgg caatggcggt
    12301 cgtgagctaa ttggggatac cctgacggcg cgcggtgctg aggtcacttt ttgtgaatgt
    12361 tatcaacgat gcgcaatcca ttacgatggt gcagaagaag cgatgcgctg gcaatcccgc
    12421 gaggtgacga cggtcgttgt taccagcggt gaaatgttgc agcaactctg gtcgctgatc
    12481 ccacaatggt atcgtgagca ctggttacta cactgtcgac tattggtcgt cagtgagcgt
    12541 ttggcgaaac tcgcccggga actgggctgg caagacatta aggtcgccga taacgctgcc
    12601 aacgatgcgc ttttacgggc attacaataa ctctcataac aggaagccat aatgacggaa
    12661 caagaaaaaa cctccgccgt ggttgaagag actagggagg ccgtggacac cacgtcacaa
    12721 cctgtcgcaa cagaaaaaaa gagtaagaac aataccgcat tgattctcag cgcggcggct
    12781 atcgctattg ctctggcggc gggcgtcggt ttgtatggct ggggtaaaca acaggccgtc
    12841 aatcagaccg ccaccagcga tgccctggct aaccaactga cggcattgca aaaagcccag
    12901 gagagccaaa aagccgaact ggaaggcatt attaagcaac aggctacgca gcttgaacag
    12961 gcgaatcgtc agcaagaaac gctggcaaaa cagctggatg aagtccagca aaaggtcgcc
    13021 accatttccg gcagcgatgc taaaacctgg ctgctggccc aggccgattt tctggtgaaa
    13081 ctcgccggac ggaagctgtg gagcgatcag gacgtcacga ccgctgcagc gttgctgaaa
    13141 agtgcagacg ccagcctggc ggatatgaat gacccgagtc tgattaccgt tcgtcgggca
    13201 attaccgatg atatcgccag cctttctgca gtatcgcagg tggattatga cggcatcatc
    13261 cttaagctta atcagctttc aaatcaggta gataacctgc gtctggccga taatgacagc
    13321 gatggttcgc cgatggattc agacggtgaa gagctttcca gttccatcag cgaatggcgt
    13381 atcaatctgc aaaaaagctg gcagaacttt atggacaact tcattacgat tcgccgtcgt
    13441 gatgacaccg ccgtaccgct gttagcgcca aatcaggata tctatctgcg cgaaaatatt
    13501 cgctctcgcc tgctggtcgc agcacaagct gtaccgcgtc atcaggaaga gacttatcgc
    13561 caggcgctgg agaacgtctc cacctgggta cgtgcttact acgatactga tgatgccacc
    13621 accaaagcgt tcctcgacga ggtggaccag ttaagccagc aaaatatctc gatggatctt
    13681 ccggaaaccc tgcaaagcca ggcgatgctg gaaaaactga tgcagacccg cgtgcgtaac
    13741 ctgctggcac aaccggcagc ggggacaacg gaagctaaac ctgcacctgc acctgcacct
    13801 gcacctgcac ctgcacctgc accgcaaggt gatactccgg cagccgcgcc gcaaggagaa
    13861 taaccatgct aaaagtgtta ttactctttg ttttgctgat tgcggggata gtggttggcc
    13921 cgatgattgc cggtcatcag ggttacgtgc tgatccagac tgacaactac aatatcgaaa
    13981 ccagcgtcac gggcctggcg attattttga ttctggcgat ggtagtgctg tttgccattg
    14041 agtggctact gcggcggatc ttccgcacag gcgcacacac ccgtgggtgg tttgtcggac
    14101 gtaagcgtcg ccgtgcccgt aagcagaccg aacaggcgct gctgaaactg gcggaaggcg
    14161 attatcagca agttgaaaag ctgatggcga aaaatgccga tcacgcggaa caaccggtgg
    14221 tgaactatct actggctgcc gaagccgcgc aacaacgtgg tgatgaagca cgcgccaacc
    14281 aacatctgga acgcgcagca gagctggccg gcaatgacac cattccggta gaaatcaccc
    14341 gtgtacgtct gcaactggcc cgtaatgaaa accatgctgc acgccacggc gtggataagc
    14401 tgctggaagt tacgccacgc catccggaag tattacgtct ggcggaacag gcgtatatcc
    14461 gcacaggtgc atggagttct ctgctggata tcatcccatc aatggcgaaa gcccatgttg
    14521 gtgatgaaga acatcgtgca atgctggaac aacaggcatg gattggcctg atggatcagg
    14581 cgcgtgccga taacggtagt gaaggtttgc gtaactggtg gaaaaaccaa agccggaaaa
    14641 cgcgtcatca ggtagcgttg caggtggcaa tggcggaaca tcttattgaa tgtgacgatc
    14701 atgatactgc ccagcaaatt atcatcgatg gcctgaaacg ccagtacgac gatcgcctac
    14761 tgctgccgat tcctcgactg aaaaccaaca atccggaaca gctggaaaaa gtgctgcgcc
    14821 agcagatcaa aaacgtcggc gatcgcccgc tgttgtggag cacactgggc cagtcactga
    14881 tgaagcacgg agaatggcag gaagcatcgc tcgccttccg cgcagcgctg aaacaacgtc
    14941 cggacgccta cgattacgca tggcttgccg acgcgctgga cagactgcac aagccggaag
    15001 aagctgcagc gatgcgtcgc gacggtttga tgttaacgtt gcagaacaac ccaccacagt
    15061 agttccttct cacccggagg caagcacctc cgggaccttt ctgatgcata aaaaaacgcc
    15121 tgctcttatt acggagcagg cgttaaaaca ggtctgtatg acaacagtgg gtgcttcact
    15181 caacgttgtg tccatggtgt ctgatgaggc ataagcgaca tctgtcagtg gacgataagc
    15241 accgtaaacg gctctgcgtc attccggagt ttatgaggca ctaaggcgaa cataagagat
    15301 ggaatgagca tctacccgtt tattatgcca cagagaatcg ggaaataaca tcccttaaca
    15361 cttgttatga gataattctg taatcctctt tgcttcctga gtgaatattt aacctgagct
    15421 tgatcctaca catacttatt atgaatgata aaattcattc aattaataac acatatacta
    15481 attgccgtta aaactaaaaa agcattaata atcaacgcaa tataataaac ccgccttaca
    15541 tatcaactgc gccagaggta ggattgaaaa cgctctcctg attttccaat tcattttctg
    15601 gataaataaa taatttattt ttgtcactat tatttatgta atcatcctgt cagggagagg
    15661 gatctcaatt atcaatgctt aattacgtca tcattttgat gacatgaaac cggagcattt
    15721 tgcatggaat tttcgttttc acccaaactt cttgttgttg ctgtcgccgc cgctcttcct
    15781 ctcatggcca gcgcagcaga taccccgtca actgccaccg cacgcaaagg ctttgccgga
    15841 tacgatcacc caaaccagta tccacagaac gctgcaattt attgaatttg cacgatcatg
    15901 taggccggat aaggcgttta cgccgcatcc ggcaatcaac cgcaggcggc cacaaaatgg
    15961 ccgctttttt acttattcac caccagcaaa tgtgcctgca taatgtcact ggccgggcga
    16021 ccgtgcgcca gcaaatcagc cattgcttta agatatggcg gtagatggcg gaaataacgc
    16081 tgatacccgg cacacaaata attcagcccc ggtttgccgc tggcatcgag catgaagcgg
    16141 tgtttcgggc agcctcccca gcacgctttt aacacgttac aactgcgaca ctgcgccggt
    16201 aattgcttaa atttatcttc accaaacacc tgctgttgcg gggaatcgat catttctgca
    16261 attgtttgct gatgcatatt ccccagccga tattgcggat aaacatagtg atcgcaggcg
    16321 taaacgtcgc cgttgtgctc aacaatcacc gagcgcccac aggttggctg atgatggcaa
    16381 accgcacccg gcgcaccgac aaaattggca aacgcccatt cgatattcat cacgaaaatc
    16441 ttgccgacgt cgcgtttgat ccagtggtcg aatatcgcca ccagaaattc accaaattca
    16501 tcggggcgca ccgaccattc cgtcagttcc ccctgaatat caccaggcgc atgcaatttc
    16561 agtccatcgc gggcagctgt ttcatcagcc agacgctcga ccaccggaat aaactggatg
    16621 aattcgactc ccgcatcgca caaaaaatca taaacctgca acggttgctg cgcgctggtg
    16681 cggttgacgc agaccagcac gttatagtcg acatgatgtt tttgcagcag cgtcagggca
    16741 cgcatcacca gcttatgcgt tggtctgcca cctttggtca cgcgatattg attgtggatc
    16801 tcagccggac cgtccagcga taacccaaca agaaaatgat tttctgccag aaatgcacac
    16861 catttatcat cgagcagcac gccgttagtc tggaagctgt tacttatctt cctgccagca
    16921 ccgtatttcg cctgtaactt tacggcacgg cggtaaaaat ccagcccgag taacgttggt
    16981 tcgccgccct gccaggtaaa agccacttcg ttttgcggtt cgctggcagc gatatagtga
    17041 cggacatacg cttccagcgt gtcatcgtcc atatgcgtga ctggcttttc gcggtaaagg
    17101 gattgttttt cgagataaaa acagtagtca cagttcagat tacaatcgga accactcggt
    17161 ttcgccatca catgaaaagc acgcgttgga acctgttgca gcatggtcac tccttgcctg
    17221 aagtcatcct tcaatacgtt aagggcgaaa tcataccgtg gggcaatgag ttattacggg
    17281 cgtttattgc gaagggaggg tgacgaaatg cacagaaaac aaaaaacccc gccgaagcgg
    17341 ggttcaaaat tggtcggcga gagaggattc gaacctccga cccactggtc ccaaaccagt
    17401 tgcgctacca agctgcgcta ctcgccgaaa tactgctttt tgaattttta gttcaattct
    17461 ttaaagtcgt ggtgcgaggg gggggacttg aacccccacg tccgtaagga cactaacacc
    17521 tgaagctagc gcgtctacca attccgccac cttcgcattg tcacaacttc taataatggg
    17581 gtggctaatg ggattcgaac ccacgacaac tggaatcaca atccagggct ctaccaactg
    17641 agctatagcc accactacaa atcttgttac gcggtattac taccaccgca gctcaagcgc
    17701 cgggactaaa tggcgcgccc gacaggattc gaacctgaga cctctgcctc cggagggcag
    17761 cgctctatcc agctgagcta cgggcgctta gcgccgttgc ggggggataa tacggacttc
    17821 ccacccctct gtctagtccc tttttaaata aattgcgcgt ttggttacgg tttgtgcgtt
    17881 ttgctgctta ttcctccagt ttatgcgctt tcccgtggcg attaaggcca aaaactttat
    17941 aaatcgccgt caccgccagc ataaagatga tgccaacaaa cagcgacata cgcgtatctt
    18001 cattaaagta catgccgatc aaaacgcaaa tcaggaatgc cattgttacg tagttggccc
    18061 acgggaacag gattgagcgg aacggatggc tggcaatcgc cgctttatgt gcacgccgaa
    18121 aacgcagctg gcttatcaat atcacaaacc atggcaccat ccccggaagc acgctggcac
    18181 tgtagacgta gacaaacaca cgctgcggat tgggaatgat gtagttcagg catgagccaa
    18241 ttagcagaat agcaatagat actgccacac ccgcaactgg tacgccgtga cgggaaactt
    18301 tcgccatcgc cgccggtaac tgacggtttt tcgccagtgc gtagagcata cgtccgcaac
    18361 tgtacatgcc gctgttacag ccagagagcg cagccgtcag caccacaaag ttgataatgc
    18421 ccgccgctgc ggtaataccg attttggcaa aagtcagtac gaacgggctg ccgttgctgc
    18481 caatttcatt ccacgggaag atggtgacga taacgaaaat cgcgcctacg tagaaaatca
    18541 ggatccgcca cagcactttg cctacagcac tgcgcagcgt cacctgcgga ttcttcgctt
    18601 caccggcagt aatgccaatc agctccacgc cctggtagga cgccaccaca atacacagcg
    18661 cggtcaggaa ccctttccag ccacccgcaa agaaaccgcc atgctctgtg agattgctaa
    18721 aaccaatcga ctgcccgcca ttgccaaagc caaagaaaat cacgcccagg ccaatgacaa
    18781 tcatcacgat aatcgtggtg actttgatca tcgcgaacca gaactcgatt tcgccgtaca
    18841 accgcaccgc cgccagattc gccaacgcca ccagcgccac cgcgatcaat gcaggtatcc
    18901 actgcgccat ctccgggaac cagaactgga cataaacgcc aatggcggtg atttctgaga
    18961 tccccaccgc catccacata aaccagtaag accaggcggt gagataacca aaaaacgggc
    19021 tcatataacg atgcgcataa acggcgaacg aaccggtaac aggttcaagg aacaacattt
    19081 cgcccattga acgcatgatg aaaaagacga acagcccggc gatgatatag gccaacaata
    19141 cggatggccc ggcccatttc agggtactgg cggcccccat aaacaggccg acgccaatag
    19201 tgccccccag ggcgatgagt tcgatatgtc gagcttccag cccacgctgt agctctggtt
    19261 tgttatctgc cataaatcct cgtgttgtgt ttgcatgctt tccggtgtta ccggttatcg
    19321 ttatgggtac atcgagtgtt gcgaatgttt tcgtaattca ggagaaatgg caaataaagc
    19381 attaaaaatt tgaatgcttt gtgtaataaa aaagcagaca ggcgacggag tgaccactcc
    19441 gtcgctttac aaagagagga aaatcagagg ttgccggtgt agtgccagcg taaataacgc
    19501 agcaaacgaa gctgacgctt aatgcggctc ggctgcgaaa gcaggcggta gagccactcc
    19561 agtcccagcg tttgccagat tttcggtgcg cgttttacgt gaccggtgaa aacatcgtaa
    19621 gtcccgccaa cgcccatata cagcgcatct ggatgcacca gacggcagtc gcgcatgaag
    19681 atctcctgct ttggcgatcc catcgcaacg gtgacgattt gcgctccgct ggcatgaatg
    19741 cgttcaaaca gcgcctgacg ctgctcgggt ttaaaataac catcctgact gccaacgata
    19801 ttcacattcc attggttgcg cagtttagct tcagtttgcg ccagcacttc aggtttaccg
    19861 cccacaagaa ataccggcgt cccttctttg cctgcgcgcg ccatcagctc ttcccagaga
    19921 tcggcaccgg caacgcggga aacctgcgcc tgcgggtact ttttacgtac tgaacgtaca
    19981 acgctgatgc catccgcata tttaaattcg gcagcgttaa ttaactccct gacctcggcg
    20041 ttatcttcaa tggtcagcat tttttcagca ttaatggcaa ccagcgttcc ctgcttaagc
    20101 tgcccgtcag caaacagata atcgagggcg tgctgcatat cacgccaacc aatcaactgt
    20161 aagccacgca gcgtataggt cggtgccgtg gtgttgttat tcattgttat ccttcaacct
    20221 gcgtccggag cgatgatttt gtacgtttat gaatgagtcc ggcgctttca aaaagccagt
    20281 acagcagttt tgcgatcatc agacatgcgc cgaagaccac gataaaaaag actacgcgtg
    20341 agacaaacga atccagccct tcacgcgcca gcacgatcat attgaagatc gccccaaagc
    20401 agaaactgtg caatatcgca gccttatagc gattaggctc gcggttgccc agctcataca
    20461 gccagtcgaa ccatttgatg atcagaccaa ccacgatcgc cccgagcggg atgaacaacg
    20521 cgccgcccat caccaccagt gagcctataa gcgtaggcga gatcgccagt ccggagtggt
    20581 tattcagcac ttcccaggta aagtagttgg ctgagttcag caccatactc gggcgacccg
    20641 gccacagcca ggaagggata aagacataga aatcgcggac aattggagcc aggccctgga
    20701 agtcgatgtt gtcgtagttc tgcaacagca acgccagatt ctcccacggg gagaaggtgt
    20761 cgcgagtgag atagagaaac gtatagaacg cttcatcgcc gctcacattc attccatagc
    20821 gttttagcgc cagccagaac atgccaacaa tccccagcac gcccgccgcc gccagcatcc
    20881 acaacgaaat ccagccgcga ataatgccaa taaacaggaa gatagcgaat gcgatgatga
    20941 tattggcgcg agtgccgccg acaatcatat aagtcagcaa gccaaaggcg accgtgctga
    21001 cgaggaaaaa cagccacgct ttgctgtcct ggcgcagaaa gtagaccacc agcatcgccg
    21061 ggatgaaaaa gtaaaagaag cgttttaacg ccacgccgga gacttcactg gagaagatct
    21121 gactgtagga gttaagccgg aacagcaaaa agccgttgtg catgaagaag atgccgacgc
    21181 ttaccagcgc gatacccatc aggatcaccc acgtaagatt ggtctccacg cggttcatgg
    21241 taaacagcgg acggcgcggt acatcagcaa cgcgtttgcg taggcgggtt ttgtaggtga
    21301 catagtaaac cgcgtagaag cagcccgcag aaagcaacgc ctgcaacaag atttctggcg
    21361 gcgcgacacc aacatcaaag cgaaatacca gcacgctggt cagcgggaag ccgaagaaaa
    21421 aggtgagcaa aaacagcaat gaaaagaaga cattgaagtt aaagcgcaca cggcgaaact
    21481 caaaccaggt cagcgtggca ataaacagcg tgcagagcag ccagaccaca aacaggccac
    21541 tgaattgcag cagactcatg cgacctcccc ggcggcaata gccaacgccc gctgccagcc
    21601 ttgtagatag ttagggctaa agaaggcaat ggtgttttta tccaccgacg ccaactggcg
    21661 ctgcgcttca cgcacaatat cctcgttgag atcgtcggta gtaaacagca ccggcaaatg
    21721 ctgttccgtc atatcctgcc agaacggatt ttcccggtta agcacacaag gaatgcccgc
    21781 ctgaatcagt aagcaaagcg taccaatgcc ctgctggcgg gcaaaaataa agtaaccaag
    21841 atcgcactga cgaagtagcg ccagataggc gtcaaattcc agtttttcgc tcaaaatttg
    21901 cagattttct tcgctgaata actccagccc cgcctgacga acttcctcaa tgtacgcttc
    21961 gttattaggc ggatatccca tcggcaccac cacttttacc gtatcgccaa attgctgatg
    22021 aacggcgcgc aaggcagcaa tatggtcatt gctgcggtca ccggagttcc ccaccagaat
    22081 ggtcattttc ccttcacgtt gccgatcatt cgccatcgta ttgagagaag ggtccatccg
    22141 cgtcgggaag tacagcagtt cgccccgcac ctttggatgc gttttggcaa aaaagctcaa
    22201 atcaccgcgg gtggcaaata cacagccgac tcgcttttgc gccaggcgac gtagcgggta
    22261 aaaaagctta tatctcaagc cactggaaag ctcatacagg tctgcccccc agatatgcca
    22321 gaaaaactgg ctgggcttaa tgccaccact cagcagagcc agccacagtg tgggattgaa
    22381 ctgaccgtgg aaaaaaaatc gctgctgacg gttagctttt gctttcgcga tgaccgcttc
    22441 cgccagcgat tttttcccag ggaaaaattg cacagaaagc gccggacagc tatcacttaa
    22501 gccgtcgtcc ttgccaacaa ccataaactc gcgcgcgtgc tcgctcgtcg cggccagcgc
    22561 gtcattgaaa aaccgcaaaa cggttcggtt atggtgaggg atatccgatc ccagtacgtg
    22621 aatcagtaca gtcatgcccg cctacgccag agtaaaaaca cgccacaaca aagagaaaaa
    22681 tagacgatat aagttgccat atatgcctgc gccgcgccca gtgcaccatg cgcagggatt
    22741 agccagtggg caaataccat caataaagtg aactggctga cttccgccag aatataaaac
    22801 cgcagtgacg ctttggcgat caccagataa ccaaagacat aagcgcccac ttttaacaca
    22861 tcacccacta actgccaggc aaagagatcg cgcatagcgg taaatttatt cgacaacagc
    22921 aaccagatag caaaatcacg cagcaaccag acggtaaaac tcgccgccgc cactgccggt
    22981 aagacgaatt tcagcgattt aaccacttcc cgggtgatat cgcgcttttc cgttagccgc
    23041 gacaacgtgg gcagcaaata tacgctgaac gatgccgtaa taaattgcag gtaggcatcg
    23101 gaaatactgc tcaccccttg ccagatcccc acttcatccc agctatactg cgccgccagc
    23161 agtttacgca tcatgatgta agcaacaggc aaggtcaccg acgtaatcaa cgccatgagc
    23221 gtaaatttgc tcaactgccc tgccagaccg ttatcccagc tgggtttcag atagcttaac
    23281 gggatgacac cacgtttgat caacatgatg gcggcaggaa tcaccaccag cgcgggaatc
    23341 agcgccagac ccagcaacgc cccttcataa ccgcccaaac ggtaactgac gtagtacgcg
    23401 agaacgccaa tcaagctgcc gacaatcagc gataacgcat tacctgcggc atcgcgaaag
    23461 cctttcatca gcgccagtaa caggttgccc caggcgatcc ccatttgcac cagcgccact
    23521 aaacgcacca gcccctgata gtcggtatta ccaaacagtc cctggctgat tggcgcagct
    23581 gccagcacaa aaaccagcgc catcagcgta gagaaaccaa gtaccatcgc tgatgaagtg
    23641 ccgaccacgc ggcgcagctg ttgcggatta tcatggtact gggcaacgta tttggttaca
    23701 ccgttaaaga tgccagctcc ggcaagcacg ccgagcacgg taatcaactg gcggaaattt
    23761 gccgccagcc caagccccgc cggaccaaat gacacagcca gcaacttacc gaccagtaac
    23821 ccggcaccaa tcttgaccag tgtactggcc gccgtccaca aggacgcttt tgccaacgac
    23881 atatcaggag aagtagttca acaaagtcgc aattaccgta cgctgattga cgggcgacag
    23941 gttgtagaac agcggcaggc gcagcaggcg ctcgctctct ttggtggtgt agcgatcttc
    24001 accgtggaac tcaccaaagc gttcccccgc agggcaaccg tgcagcggaa tgtaatggaa
    24061 caccgccatg atttccgctt ctttcagaaa gttaatcaac gcgctccggt catcaatatc
    24121 ccgcagttta atgtagaaca tatgcgcgtt ctgcacgcag ccatcgggaa tcgacggcag
    24181 ctcgatacgc ccggctttcg ccagaggcgc taacgcatcg tagtagtttt gccacagcgc
    24241 cagacgttgc tggttgatac gatccgctgc ttccagttgc gcccacaggt acgcagcttg
    24301 cagatcggac atcaaatagc tggagccaat atcgcgccag gtatatttat cgacctgacc
    24361 acggaagaac tggctgcggt tagtgccctt ttcacggatg atctcggctc gttcgattaa
    24421 cgctttatcg ttaatcagcg tcgcgccgcc ttcaccaccc gccgtatagt ttttggtttc
    24481 atggaagcta aagcagccaa tatgaccaat ggttcccagt gcacgccctt tgtaagtgga
    24541 catcacgccc tgagcggcat cttctaccac aaacagatta tgctttttcg ccaacgccat
    24601 aatggtgtcc atttcgcagg ccacacccgc gtaatggacc ggcacgataa cgcgcgtttt
    24661 gtcggtgatc gccgcttcaa tcagcgtttc gtcgatgttc atggtgtccg ggcgaacatc
    24721 cacaaaaacg atttttgcgc cacgcagcac aaaggcattg gcggtggaga caaaggtgta
    24781 gctcggcatg atcacttcat cgccaggctg gatatcgagc agcagcgccg ccatctccag
    24841 cgaagcggtg caggacggcg tcagtaacac tttggcgctg ccaaaacgtt gctccagcca
    24901 ctgctggcag cgacgggtaa aaccgccatc gccacacagt ttgccgctac ccattgccga
    24961 ctgcatatag tcgagttcgg ttcccaccac cggcggtgcg ttaaatggaa tcatgtgatc
    25021 acctgtataa ccagtacgcg gtgctttcta cattcgcacc actttgtatg tatcgtttaa
    25081 gcgcggcggt gttgcccatt tgggtcgcca cccgcaaagt tgttttaccg cgagcatacg
    25141 cccagtttag cgccgtttgc atcagctcag cacctgcacc gcgtccagcc agcaggccaa
    25201 ttcgcgcatc tgtcgcattg agttcccgta aagagacata accgcgaata ccgccggacg
    25261 ccgcacgtaa aatcagacat tgatgatcaa aggtgccgcg cacggcattt tcaatccact
    25321 gtgcataaaa gcgtccgctg gcgtcaggcg cataccacgg cgcacgaaaa cggctttgcg
    25381 caaatgcggc gctggctaac tgacgtaatg cgggaatatc ggtctcttgt gccactacag
    25441 caccactatc actgacattg ttcacgggta gtgccaaatc aacttcacct tctaccaggg
    25501 agaatcccag ctgttgcagg gcatccagtt cacccgtatt tgatgccgca attttggcct
    25561 gcacccgtga ccacggcgct aacacgtctg gcgtcaggag ctgtgcttca gacgtaatgc
    25621 gcacgatggc gctgttaaca ccaaagaagg cgttttccca ggttagtggc tcaatactgg
    25681 cgcggacggg cacgaagtaa ctccagcaga tattggccgt agccagtttt cgctaatgaa
    25741 ctggcagcac gcttcacacc ctcgtcatcg agccagccgt tacgccaggc gatctcttcc
    25801 aggcaggcaa tcttaaagcc ctggcgtttt tctactgtct gcacaaaggt actggcttca
    25861 atcaggctgt cgtgagtgcc agtgtccagc caggcaaatc cgcgcccgag cagttcaacg
    25921 gtcaggttgc ccgcctcgag gtacatctgg ttgatggagg taatctccag ttcaccacgc
    25981 tccgacggct tcacctgctt tgcgtactcc acgactttac tgtcgtagaa ataaagcccg
    26041 gtcaccgccc agtttgactt cggctgtttt ggcttttctt ccagcgagat agcgcggaaa
    26101 ttgtcgtcaa actccaccac gccaaagcgt tccgggtcca tcacctgata gccaaatacc
    26161 gtcgcccctt ccgtgcgcgc cgcaacatga cgcagcttcg gactgaagcc ctgaccgaag
    26221 aagatgttat cgcccagcac cagacaagaa ggttcaccgt tgaggaaggt ttcaccgatg
    26281 ataaaggcct gcgccagacc gtccgggctg ggctgttcgg cgtattccag ctggataccg
    26341 aactcactac catcgcccag caggcgctgg aaataacctt tatcttccgg cgtagtgatg
    26401 atgagaattt cgcggatatc ggccaacatc agcaccgaca gcggatagta aatcattggc
    26461 ttatcgtaaa tcggcaacag ttgcttcgat acgccgcgcg taatcggatg caatcgggtg
    26521 ccggaaccgc ccgccaggat aatacctttc atgtttgcct ccgctgaaaa ttagcctttc
    26581 agacctaaac gctcgccctg atagctgccg tcctgcacct gcttccacca gctttcatta
    26641 gccagatacc actgcaccgt tttacgcatt ccactttcaa aggtttcctg tggcagccag
    26701 ccaagttcac gggcaatttt cgaagcatca atggcataac gcagatcatg ccccggacgg
    26761 tcagcgacaa aggtgatcaa gtcacgatag tgcaccacgc cgtgcggctt gttcggagcc
    26821 agttcttcca gcagctcgca aatggtttcc acaacatcga gattcttacg ctcgttgtga
    26881 ccaccaatat tataggtttc accgactttc ccggtggtcg ccacgcaata cagcgcgcgg
    26941 gcgtgatctt ccacatacag ccagtcacgg atttgctgcc cgttgccata taccggcagc
    27001 ggtttacccg ccagcgcgtt gaggatcatc agcgggatca gtttttccgg aaagtggtaa
    27061 ggaccgtagt tattcgagca gttggtgata agcgtcggca gaccgtaggt ccgcagccag
    27121 gcgcgcacca gatggtcgct gctggctttt gacgcggaat aagggctgct cggcgcatac
    27181 ggcgtggttt cggtgaagaa atcatccgtc gagtgcaggt caccatatac ttcgtcggtg
    27241 gagatatgat gaaaacggaa cgctgatttt ttatcttccg tcagcgtatt ccagtaagcc
    27301 cgcgccgctt caagcaatgt ataagtcccg acaatgttgg tttcaataaa cgctgccggg
    27361 ccgtcaatag aacggtcaac atggctttcg gctgccagat gcatgacaca gtctggctga
    27421 tgctcagtga atacgcgtgc cagttctgcc cgatcgcaga tatcaacttt ctcaaaggca
    27481 aagcgctcgc tttgcgcgac cggtgccagc gacatcaggt ttccggcgta ggtcagctta
    27541 tcgaccacta ccaccgcgtc gctcgtttcg ttgatgatat aacgcaccag cgccgagcca
    27601 ataaacccgg caccacctgt tatcagaatt tttctcatca gcgccagact cctttggcat
    27661 cgacgacata ctgctgatgg acattgtcgc cattgataac tttaaactga ctatgatcga
    27721 ccagcatcac cagcacgtct gccgttgcca gcgcctcgtc aagctgcgcc agagtacaaa
    27781 gcccggtcag ttttttcggc aactggtgga tgttaggctc aacaaccaga gtttcgccgc
    27841 tatgccactg ggcgatcagt tcagcgattt ccatcgccgg gctttcgcgc aggtcatcaa
    27901 tattcggttt aaacgccaga ccaaagcagg cgattttcag ttcactggcg cgtttatcgg
    27961 tagccgccag gcaatcagcc accgccgctt tcacctgatc gataacccag aacggtttgt
    28021 gatcgttcac ttcgcgcgcg gtacggataa gccgcgcctg ctgggggttc tgtgccacga
    28081 taaaccacgg atcaacagca atgcagtgac cgcccacgcc agggccaggc tgaagaatat
    28141 taacgcgagg gtgacgattc gccaggcgaa tcagttccca gacgttaatc ccctgatcgg
    28201 cacaaatcag cgacaattca ttagcaaaag cgatattcac atcgcggaag ctgttttcgg
    28261 tgagcttaca catttccgcc gtccgcgagt tagtgacgac acactcacct tcgaggaaaa
    28321 ttttgtacag ttcgctggcg cgggtcgaac aaaccggcgt cataccacca atcacgcgat
    28381 cgtttttaat cagctcgacc ataacctggc ccggtaacac gcgttccggg cagtaagcaa
    28441 tgttgacgtc cgcctgctcg cccacctgct gtgggaaagt gagatccgga cgcatctctg
    28501 ctaaccattc tgccatcttc tcggttgacc ccaccggcga ggtggattca aggatcacca
    28561 gcgcgccttt tttcagcact ggcgcaatgg agcgagcagc cgattcaacg taggtcatat
    28621 ctggctcatg atcgccttta aacggcgtgg gtacagcaat cagccaggca tccgcttcaa
    28681 ctggcgtcgt gctcgctcgt aaaaaaccgc cttctacggc agtttttact acgctcgcca
    28741 aatcaggctc gacgatatgg atttcgccac gattgatggt atcaaccgcg tgttggttga
    28801 tatcgacacc aatcacctgt ttttgccgtg aggcaaacgc tgctgctgta ggcagcccga
    28861 tgtaacccag tccgataaca gaaatggtcg caaaactcat agtgatatcc gattattttt
    28921 taacgcttcc agaatgcgag agcatgcctg accatcacca tacgggttat gggcgcggct
    28981 catagtttga tattcgtttt cgtcttttaa aagacgcgtc acttcctcga caattcgctg
    29041 cttatccgtg cctaccagac gcaccgtacc cgccgtcacc gcttccggac gctcagtggt
    29101 atcgcgcatc accagcaccg gtttccccag cgaaggcgct tcttcctgaa tgccgcctga
    29161 gtcggtcaaa atcagccagg cgtggttcat cagccagaca aacggtaaat actcctgggg
    29221 atcgatcaga atgacatttt tcacatgccc cagaatgcga ttgaccggct ctctgacgtt
    29281 cgggttgaga tgcaccggat agacaatctg gatgtcctgg tgcgtggtgg caatgtctgc
    29341 caacgcgtgg cagatttctt caaagccacg accgaaactt tcacgcctgt gaccggtcac
    29401 cagaatcatc tttttatcgg ggtcgataaa cgggtaattt gccgccagtt ctgaacgcag
    29461 cgtatcgctg ctcatcacct ggtcacgcac ccataacagt gcatcaataa ctgtattacc
    29521 agtaatgaag attcggctat ccgcaacgtt ttcacgcagc aagttttgcc gggaagtttc
    29581 ggttggagag aagtgataca tcgccagatg cccggtcaat gtacggttag cctcttccgg
    29641 ccacggcgaa taaagatcgc ccgtgcgcag accagcctca acgtgaccaa caggaatacg
    29701 ctgataaaac gccgccaggc tggttgccag cgtcgtcgtt gtgtcgccgt gaaccagcac
    29761 gacgtctggt ttgaactcgg caagaatagg ttttagccct tccagaatcc gacaggttat
    29821 ctctgtcagg ccctgtcctg gctgcattat gttgagatcg tagtcaggta caatggaaaa
    29881 gagtttcagc acctgatcga gcatctcccg atgctgcgca gtgacgcaaa ctttagcctc
    29941 aaaaaaagga tcttttgcca acgcatgcac caacggcgcc atcttgatgg cttccgggcg
    30001 cgtaccaaat acagtcagta ctttcacatc gattctcttc gaataagcgg cgagcgcctt
    30061 tgcgctcacc gcagcagtgt tgctatttcg agcaacggcg ggttaatgcg acaccagccc
    30121 cgatcagccc cccgacaatg ccccacataa tcatcaggaa ggcacgacgt gggctatcgc
    30181 gttttaccgg ttcttccggc gtacgcaaat agcgataggt ctgaaaacgc ggatccaggg
    30241 ttggaccaac attcagggtg tttaacatgg cccgattctg atcatagtcg agatcaaagg
    30301 ccggaccgac ggcctgtaaa ttttccagtc gagcctggag cattggacgc ccaagcagga
    30361 acatttctga atcaggtaat tcctcggcag gcacatctgt cgcactgcgc gaaatattat
    30421 gctgctcagc aattttcagc gcctgttcaa tactgttcat ccggcggtcg tagatggctt
    30481 tcgccacctc ttcctgacgc ttcacctgtg ctttcatctg gatggtacgc gccgcccatg
    30541 cgcctttcag ctcatcattc agatggctgg ctgcacgctg gctggcaaaa gcaacatact
    30601 gacgtaacag gttattagcg tcaggcgcgg tttcagcaat aagcttcacg ctgtcattga
    30661 ccgcgcgggt aaagtctccg gggataaact ggatgttgtt aatcatttca tccagcaacg
    30721 ccgcatcggc tttgctgttg cccaccatcc gctgtttgta atagtcggtt tgcagccaga
    30781 actctctgcg ggtatcccac gaggccagtt gcataacaaa ctctttatag gcttcgtcca
    30841 tgaccgatgg ttggtcggca gaagccatgt ttgaacggac atccaggtta cgcaaaaatt
    30901 gctgctgcga gtaatatccc cccagcatat tcaccgttgg acgatcggta atcgccgtcg
    30961 agctccactc ctgacgagca aaaaaagtat acgccagcgc gattaacgca aatgccagcc
    31021 ccatgccaat aatccatagc ttcccagccc acaaggtacg aaacaaccca cgaatatcca
    31081 gttcattttc agcgtcttcg gccggtttcc caggcattgg ttgtgtcatc acatcctcat
    31141 ttatttggtt aaattggggc tgccaccacg atttctacgc agtctgcgtt ttacgcgctt
    31201 aataaagcga gcaactttcc aggcacgctt aatgcaatat ccatagagga agaatgctag
    31261 caaaaagagc accagcatga cccactccgg gacaaaatga gaatattctg ccagcacgcc
    31321 aatggaagcg agcagtgccg cagcaagggt aatcagcaca aacgcctgac gggaagtaaa
    31381 cccggcacgc atgatcaaat ggtgaatatg ctgacggtca ggagagaatg ggctcatgcc
    31441 tttacgcagg cgacggtaca taatcgccac catatccatt agcggaatgg cgattatcca
    31501 caaagcggta accgggctga tgggatgggt tttgccctgg gtcgtttcga gcaggatcca
    31561 gataacggta aaaccaatca gcgtactgcc cgcatcaccc ataaagactt tgtagcggcg
    31621 acccaggata ccaaggttaa gcatgatgta tggcaggatg gcggcgatca tcgcaaagca
    31681 ccagattgcg aggctggttt gcccgtcgaa ccacaaaatc ataccgattg ctgcaaacga
    31741 gacgcaggac aacccgccca gcaagccatc aatgccatca accatgttga acgcattaat
    31801 ggccgcccag acggcaaata gcgtcaggaa gtaaccaaac ggtccgagca ccatctccca
    31861 ggagccaaag atataaccca ggctactgag ataaagtttg ccgaacacca tcataacaat
    31921 gccaacagcg gcctgtatgg tggcacggat ttttacgctg atatcaaaac ggtcatccag
    31981 cgcgccaatg aaaacaagca caccggcaca agcgagatag agagatgcat gcggaatata
    32041 gtaatcgaca attccgaacg tgaagcaaat ccctgcgtaa accgaaatcc ccccaacgag
    32101 aggtatcaat ccctggtgac gtttgcggaa gtttggttta tccactaaac cgactttttt
    32161 tgccacctta cgggcaaaaa acagaaacag tgtcgtgaat aaaaaaatac tgatgagatc
    32221 agtactcact gtcagtaaat tcacaatgca tgctctcaga gaaaattatt agcagaagta
    32281 taaccacgaa gacctttatt cagaagggaa agtccgttcc gaacccacca gtcctgtgca
    32341 aaaattaacc atttgatatg caaattgcat aacacgccag caaattccag gaattttcct
    32401 aacatcgcca gcgcggcatt aagattacag atataaaagc aaaacgccac gtaaacacgt
    32461 ggcgtttttg gcataagaca aatttatgag cgtttcatca tttcgaagaa atcgtcattg
    32521 gtcttggtca tggccagttt attaatgagg aattccattg catcgatttc gcccatcggg
    32581 tgaatgattt tgcgcaggat ccacattttc tgcagttctt cctgagtagt gagcagttct
    32641 tctttacggg taccggaacg gttgtagtcg atagccggga agacgcgttt ttcagcgatc
    32701 ttacgagaga ggtgcagttc catgttgcct gtacctttaa actcttcgta gataacttcg
    32761 tccattttag aaccggtatc gataagcgcc gtcgcgataa tagtcaggct gccgccctct
    32821 tccacgttac gcgccgcacc aaagaagcgt ttcggacgat gcagggcgtt ggcatccaca
    32881 ccaccggtca acactttacc tgacgccgga acaacggtgt tgtaagcgcg cgccagacga
    32941 gtgatggagt cgagcagaat gataacgtct ttcttgtgct caaccaggcg tttggccttc
    33001 tcgatcacca tttccgcaac ctgaacgtgg cgagatgcgg gttcgtcaaa ggtagaagca
    33061 acaacttcac cttttaccag acgctgcatc tcggttactt cttccggacg ttcgtcgatc
    33121 agcagaacca tcagcacaca atccgggtgg ttgtaagcaa tgctctgggc aatgttctgc
    33181 agcagcatgg ttttaccggc tttcggcggt gccacaatca gaccacgctg accacgaccg
    33241 ataggtgatg ccagatccag tacgcgagca gttaaatctt cagtagaacc gttaccacgt
    33301 tccatacgca gacgagaatt tgcgtgcagc ggggttaagt tctcaaagag gattttgttg
    33361 cgggcgtttt caggtttgtc gaagttaact tcgttaactt tcagcagcgc aaaatagcgt
    33421 tcaccttctt tcggcgggcg aatcttacca gagatggtat caccagtgcg gaggttgaaa
    33481 cggcggattt ggctagggga aacgtagatg tcatcaggac cggcgaggta ggagctgtct
    33541 gcggaacgga ggaaaccaaa tccatcctgc aatatctcca gtacgccatc accaaagata
    33601 tcttcgccac tctttgcgtg ctgcttcagg atggcaaaaa taatgtcctg cttacgcatg
    33661 cgagccaggt tttccagccc catattttcg ccgagagtga tcagctcaga aaccggcgta
    33721 ttctttaatt cggtaagatt catagtggtg tgagttctta aacttggggt aaatctcgaa
    33781 cttaatgttg tgaatggtat ggcaggatca tccatgcctg tttacggtca tcgactcatg
    33841 tcttttcgct gcctggtcac aggagagtac gcagaactga aacgacaaga cggattgagt
    33901 gacgagccag aaatctgttc acttcgcatt taagataaaa cgggaagcgt tggaaattac
    33961 aagattcaaa cttaataagg tatgtttaat acgaagtcaa cactaagtta gcatgactca
    34021 cgccgggcgt ccagttttta gcgacggggc acccgaacat gaaattccct tacgccaggt
    34081 tagcgtcgag gaactctttc aactgacctt tagacagtgc gcccactttg gttgccgcca
    34141 cttcaccgtt tttgaacagc agcagagtcg ggataccacg gatgccatat ttcggcgcag
    34201 tgccagggtt ttgatcgatg ttcagttttg caacggtcag tttgccctga tattcgtcag
    34261 cgatttcatc cagaatcggg gcgatcattt tgcacggacc gcaccactct gcccagaaat
    34321 cgacgaggat cgccccgtcc gctttgagta catccgtgtc aaaactgtcg tcagtcaggt
    34381 gaataatttt atcgctcata tataactcca caggaataag cctggcgtgt tggtttcgtt
    34441 gttggtgtaa cattaaccaa ctaaaggttg actttatttc accggatacg ctttcgtaaa
    34501 gcaatagtaa gctgatattc taccacacta tgagcaaaac acatttaaca gaacagaagt
    34561 tttccgactt cgccctgcat ccgaaggttg tagaagccct tgaaaaaaaa gggtttcata
    34621 actgtacgcc cattcaggca ctggcccttc cgctgacgct ggcgggtcgt gacgtagccg
    34681 ggcaggcgca aaccggtacc gggaaaacga tggcgtttct tacgtcaacg tttcattatc
    34741 ttctctctca tcctgcgatt gccgatcgca aggtgaatca accgcgtgcc ttaattatgg
    34801 caccgacgcg tgaacttgcc gtgcagatcc atgccgacgc agaaccgctg gcggaagcta
    34861 ctggcctgaa gctgggtctg gcttacggtg gtgatggcta cgacaaacag ctgaaagtgc
    34921 tggaaagcgg cgttgacatt ctgattggca ccacgggtcg tttaattgac tacgccaagc
    34981 agaaccacat taacctcggt gccattcagg tagtggttct ggatgaagcc gatcgcatgt
    35041 acgatctggg ctttattaaa gatatccgct ggctgttccg ccgtatgccg cctgcaaacc
    35101 agcgcctcaa catgctgttc tccgccacgc tttcgtaccg ggtacgtgaa ctggcgttcg
    35161 agcagatgaa caatgccgaa tatattgaag tggaaccgga acagaaaacg ggccaccgta
    35221 ttaaagaaga gcttttctac ccttctaacg aagaaaaaat gcgtttgctg caaacgctga
    35281 tcgaagaaga gtggccagac agagcgatta ttttcgccaa taccaaacac cgttgtgaag
    35341 agatctgggg ccacctggcg gcagatggtc atcgtgtcgg tttgttgaca ggcgatgtcg
    35401 cgcagaaaaa acgtctgcgt attcttgatg aatttacccg tggcgatctg gatattctgg
    35461 ttgccaccga cgttgccgcg cgtggtttgc atattccggc agtgacgcac gtctttaact
    35521 acgatttacc cgatgactgt gaagattacg ttcaccgtat tggtcgtaca ggtcgcgcag
    35581 gcgcaagcgg tcactctatc agcctggcgt gtgaagagta tgcattgaat ttgcctgcta
    35641 ttgaaaccta tattggtcac tcaattccgg taagcaaata caatccggac gcattgatga
    35701 ccgatctgcc aaaaccgctg cgcctcacgc gcccacgtac aggcaatggt ccgcgtcgta
    35761 ctggcgctcc gcgtaatcgt cgtcgttcag gttaaataaa atgtcatact caaaatcatt
    35821 caaactgtat caagatggca agagaatgaa tcccgatgag catactgtag taagtgattc
    35881 aggtgaaaga atgcagccaa cacagagaca gattgaagga tgaagagtat gggttccacc
    35941 tcgtcgctgt atgcagccat tgatctcggt tcgaatagtt ttcatatgct ggttgtgcgc
    36001 gaggtggctg gaagcatcca gacgctgacg cgaataaaac gcaaagtgcg tctggctgct
    36061 ggcctgaata gcgaaaatgc cctgtctaat gaagcaatgg agcgcggttg gcaatgtctg
    36121 cgcctgtttg ctgaacgtct gcaagatatc cctccctcgc aaattcgcgt tgtcgctacg
    36181 gcgacattac gtcttgccgt caatgcgggt gattttattg ccaatgcgca ggaaatcctc
    36241 ggttgtccgg tacaggtgat cagcggtgaa gaggaagcac gtctgattta tcagggcgtt
    36301 gctcacacaa ctggcggtgc cgatcagcgc ctggtggtgg atataggcgg tgccagtact
    36361 gaactggtaa ccggcacggg tgcacaaacc acctcgttgt tcagcctgtc gatgggctgc
    36421 gtcacctggc tggaacgcta ttttgccgat cgtaatctgg ggcaggaaaa ttttgatgct
    36481 gcagaaaaag cggcacgcga agtgttacgt ccggttgccg atgaattacg gtatcacggc
    36541 tggaaagtgt gcgttggtgc ttccggcacc gtgcaggcgt tacaggaaat catgatggca
    36601 caggggatgg atgaacgcat taccctggaa aagttgcagc aattgaaaca gcgagccatt
    36661 cattgcggtc ggctggagga actggagatt gacgggctga cgctggaacg tgcgttagtg
    36721 ttcccgagtg gtctggcgat cctgatcgcc atttttaccg aactgaatat tcagtgtatg
    36781 accctggcgg gcggtgcgct gcgtgaaggc ctggtctacg gcatgttgca tctggccgtc
    36841 gagcaggata ttcgcagccg tacgctgcgt aatattcagc gccgctttat gatcgatatt
    36901 gatcaggcgc agcgcgtagc caaagttgcg gctaacttct tcgatcaggt ggaaaaggaa
    36961 tggcatcttg aagcaataag ccgcgatttg ctcatcagcg cctgtcagct tcatgaaatc
    37021 ggcctgagcg ttgactttaa acaagcgccg caacacgctg cttatctggt gcgtaatctg
    37081 gatcttcccg gttttacccc cgcacagaaa aaattgctgg cgacgctact gctcaaccag
    37141 actaatccgg tcgatctctc atcgctgcat cagcaaaatg ccgtaccgcc gcgcgtcgca
    37201 gaacaactct gccgtttact ccgcctggcc atcatttttg ccagccgtcg ccgtgacgat
    37261 ctcgtgccag agatgacatt acaggctaac catgaactgt tgaccttgac gcttccgcaa
    37321 ggttggctaa cccaacatcc gctgggtaaa gagattattg ctcaggaaag ccagtggcag
    37381 agctatgtcc actggccgct ggaagtgcat taatgagtaa gtgccggatg cgatgctgac
    37441 gcatcttttc cggccttgat tatttccctc gttttgccgc catcatcgct ttcagattcg
    37501 ccagatggct ttgccctttc tgcatccgtt cttcggcgct gaccactttg cgctcctgtt
    37561 cccaaatcag atcatcctgc ggcagctcca gcaaaaagcg gctcggctcc gggcgcacca
    37621 gctcgccgta ctgacggcgt tctttacaca gcgtaaaggt caattccttc tgggcgcggg
    37681 taatgccgac ataggccagc cgccgctcct catcgatatt atcttcatcg atgctgctct
    37741 ggtgcggcaa aaacccttct tccataccga ccatgtagac ataaggaaac tccagccctt
    37801 ttgacgcgtg cagagtcatc agttgcacct gatccagctc ttcttcactc tcaccacgct
    37861 ccatcatgtc gcgcaaagta aagcgcgtca ccacctgggt gagcgtcatc ggctcatcca
    37921 gttcgctgcc ttccagcatc tccgtcatcc agctaaacag ttggttgacg ttcttcatgc
    37981 gcatttcggc ggctttcggg ctgggcgatg tttcgtacag ccaggattca taatccatgc
    38041 cgtggatcag atcgcgcacc gcggcaatcg gctcccgctc cgccagacgc tggatttctg
    38101 ccaaccagtg agtaaagcgg gtcaatgctt cataaccacg tccgctaagc gtctgactca
    38161 ggcccatatc aaagctggcg gtaaacatgc ttttattgcg cgtcatcgcc cactcaccca
    38221 gctttttcag cgtcgccggg ccaatctctc tcttcggcgt gttaacgata cgcagaaatg
    38281 cgctgtcatc gtccgggtta gtcagcacgc gcagataagc cagcaagtcc ttgatttcag
    38341 gacgagagaa aaacgacgta ccaccagaaa ttttgtacgg gatgcggttt tgcatcagga
    38401 atttttcaaa cactcgcgac tgatggttac cgcgataaag aatagcgtaa tctttgtact
    38461 gcgttttatt gacgaagtga tgcgcaatca gttcaccagt gacgcgctcg gcttcatgtt
    38521 cttcgttatt cgcgcttaaa actttaagct ctgtgccgta acccagttcg gagaacagac
    38581 gcttctcaaa gacgtgcggg ttattggcga tcaggatgtt cgccgctttc aaaatacgtc
    38641 cggaagagcg gtagttctgc tcaagcttaa tcaccttcag cgccggaaaa tcctgactca
    38701 gcagcaccag gttttgtgga cgtgcgccgc gccaggagta gatcgactgg tcgtcatcgc
    38761 ccaccacggt aaagcgcgcg cggctgccca ccagcagttt caccagttca tactggctgg
    38821 tgttggtgtc ctgatactca tccaccagca gatagcgaat tttgttctgc cagcgctcgc
    38881 ggacttcttc attgcgttgc agcagcaacg tcggcaataa aatcagatca tcgaagtcga
    38941 gaacattgca cgctttcagg tgtgcatcat acagcccata acaatgggca aaaatacggt
    39001 cccgctcgcc aatcgcactg gctgccgcct gggacggtgt tttgagatcg ttcttccagt
    39061 tagagatggt cgaaatcaac tgttgcagga gaactttgtc atcttcaatc agcccttcgg
    39121 tcaactcttt gagcaacgcc agctgatcgg tatcgtcaaa cagcgaaaag ttcgccttca
    39181 tccccagcgc cgcatactca cgcttgatga tatccagccc cagcgtgtgg aaggttgaga
    39241 tcatcagccc acgcgcctct ttgcgaccca gcgtctgtcc gacacgctct ttcatctcgc
    39301 gcgccgcctt attggtaaag gtcaccgccg cgatatgtcg cgcctgataa ccgcaaccgc
    39361 ggatcagatg ggcgatttta ttggtgatca cacgagtttt accggaaccc gcgcccgcca
    39421 gcaccaggca ggggccggta acgaattcga cagcttgttg ttggccgggg tttagacgca
    39481 taggtgtatt gctcaatctt cgaacggggg gaggattgta gcagaatcgc atttgaatgt
    39541 gaccgcgtgt aagccgctaa ccgattggct gcggatgttt aaatcagaga gggaagaccg
    39601 aaaccggagt tcatcgtgaa tccattagac ttagaaaata tcgggtcgta acgttgtgtt
    39661 attacttttt cagagtttac taaggagagg tgtactcgtg gcgattaatt tcagtcctaa
    39721 ggttggtgaa atactggatt gcaactttgg taactatcct gttagccaga atggacagtt
    39781 cagcaccacg tattacgatg gccgcattcc tcctgaaatg ataaaaaaca ggttagt
//
LOCUS       DAETQN010000022        39748 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.022, whole genome shotgun sequence.
ACCESSION   DAETQN010000022 DAETQN010000000
VERSION     DAETQN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.022"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..268)
                     /locus_tag="K0W68_001215"
     CDS             complement(<1..268)
                     /locus_tag="K0W68_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3072828.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPV"
     gene            complement(428..640)
                     /gene="rpmE"
                     /locus_tag="K0W68_001216"
     CDS             complement(428..640)
                     /gene="rpmE"
                     /locus_tag="K0W68_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBI3072829.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            843..3041
                     /gene="priA"
                     /locus_tag="K0W68_001217"
     CDS             843..3041
                     /gene="priA"
                     /locus_tag="K0W68_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBI3072830.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3197..4222
                     /gene="cytR"
                     /locus_tag="K0W68_001218"
     CDS             3197..4222
                     /gene="cytR"
                     /locus_tag="K0W68_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBI3072831.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4314..5273
                     /gene="ftsN"
                     /locus_tag="K0W68_001219"
     CDS             4314..5273
                     /gene="ftsN"
                     /locus_tag="K0W68_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBI3072832.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5366..5896
                     /gene="hslV"
                     /locus_tag="K0W68_001220"
     CDS             5366..5896
                     /gene="hslV"
                     /locus_tag="K0W68_001220"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBI3072833.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5906..7237
                     /gene="hslU"
                     /locus_tag="K0W68_001221"
     CDS             5906..7237
                     /gene="hslU"
                     /locus_tag="K0W68_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBI3072834.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7304..8230
                     /gene="menA"
                     /locus_tag="K0W68_001222"
     CDS             7304..8230
                     /gene="menA"
                     /locus_tag="K0W68_001222"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBI3072835.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8323..8808
                     /gene="rraA"
                     /locus_tag="K0W68_001223"
     CDS             8323..8808
                     /gene="rraA"
                     /locus_tag="K0W68_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBI3072836.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8893..9138)
                     /gene="zapB"
                     /locus_tag="K0W68_001224"
     CDS             complement(8893..9138)
                     /gene="zapB"
                     /locus_tag="K0W68_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBI3072837.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9564..10409
                     /gene="glpF"
                     /locus_tag="K0W68_001225"
     CDS             9564..10409
                     /gene="glpF"
                     /locus_tag="K0W68_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HBI3072838.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10432..11943
                     /gene="glpK"
                     /locus_tag="K0W68_001226"
     CDS             10432..11943
                     /gene="glpK"
                     /locus_tag="K0W68_001226"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBI3072839.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            12073..13083
                     /gene="glpX"
                     /locus_tag="K0W68_001227"
     CDS             12073..13083
                     /gene="glpX"
                     /locus_tag="K0W68_001227"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBI3072840.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13180..13926
                     /gene="fpr"
                     /locus_tag="K0W68_001228"
     CDS             13180..13926
                     /gene="fpr"
                     /locus_tag="K0W68_001228"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBI3072841.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13931..14359)
                     /gene="uspD"
                     /locus_tag="K0W68_001229"
     CDS             complement(13931..14359)
                     /gene="uspD"
                     /locus_tag="K0W68_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBI3072842.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14386..14685)
                     /locus_tag="K0W68_001230"
     CDS             complement(14386..14685)
                     /locus_tag="K0W68_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBI3072843.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14897..15337)
                     /locus_tag="K0W68_001231"
     CDS             complement(14897..15337)
                     /locus_tag="K0W68_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBI3072844.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15438..16037
                     /locus_tag="K0W68_001232"
     CDS             15438..16037
                     /locus_tag="K0W68_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBI3072845.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            16145..16912
                     /gene="tpiA"
                     /locus_tag="K0W68_001233"
     CDS             16145..16912
                     /gene="tpiA"
                     /locus_tag="K0W68_001233"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBI3072846.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16967..17722)
                     /gene="cdh"
                     /locus_tag="K0W68_001234"
     CDS             complement(16967..17722)
                     /gene="cdh"
                     /locus_tag="K0W68_001234"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBI3072847.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17829..18818)
                     /gene="sbp"
                     /locus_tag="K0W68_001235"
     CDS             complement(17829..18818)
                     /gene="sbp"
                     /locus_tag="K0W68_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBI3072848.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19138..20100)
                     /gene="pfkA"
                     /locus_tag="K0W68_001236"
     CDS             complement(19138..20100)
                     /gene="pfkA"
                     /locus_tag="K0W68_001236"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBI3072849.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20281..21183)
                     /gene="fieF"
                     /locus_tag="K0W68_001237"
     CDS             complement(20281..21183)
                     /gene="fieF"
                     /locus_tag="K0W68_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBI3072850.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21331..21831)
                     /gene="cpxP"
                     /locus_tag="K0W68_001238"
     CDS             complement(21331..21831)
                     /gene="cpxP"
                     /locus_tag="K0W68_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBI3072851.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21981..22679
                     /gene="cpxR"
                     /locus_tag="K0W68_001239"
     CDS             21981..22679
                     /gene="cpxR"
                     /locus_tag="K0W68_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBI3072852.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22676..24049
                     /gene="cpxA"
                     /locus_tag="K0W68_001240"
     CDS             22676..24049
                     /gene="cpxA"
                     /locus_tag="K0W68_001240"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBI3072853.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24155..24829)
                     /gene="yiiM"
                     /locus_tag="K0W68_001241"
     CDS             complement(24155..24829)
                     /gene="yiiM"
                     /locus_tag="K0W68_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBI3072854.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24978..25961)
                     /gene="kdgT"
                     /locus_tag="K0W68_001242"
     CDS             complement(24978..25961)
                     /gene="kdgT"
                     /locus_tag="K0W68_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBI3072855.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26221..26841)
                     /gene="sodA"
                     /locus_tag="K0W68_001243"
     CDS             complement(26221..26841)
                     /gene="sodA"
                     /locus_tag="K0W68_001243"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBI3072856.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27126..28160
                     /gene="rhaT"
                     /locus_tag="K0W68_001244"
     CDS             27126..28160
                     /gene="rhaT"
                     /locus_tag="K0W68_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HBI3072857.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28157..29005)
                     /gene="rhaR"
                     /locus_tag="K0W68_001245"
                     /pseudo
     CDS             complement(28157..29005)
                     /gene="rhaR"
                     /locus_tag="K0W68_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(29079..29915)
                     /gene="rhaS"
                     /locus_tag="K0W68_001246"
     CDS             complement(29079..29915)
                     /gene="rhaS"
                     /locus_tag="K0W68_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBI3072858.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30203..31672
                     /gene="rhaB"
                     /locus_tag="K0W68_001247"
     CDS             30203..31672
                     /gene="rhaB"
                     /locus_tag="K0W68_001247"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBI3072859.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            31669..32928
                     /gene="rhaA"
                     /locus_tag="K0W68_001248"
     CDS             31669..32928
                     /gene="rhaA"
                     /locus_tag="K0W68_001248"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBI3072860.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            33170..33994
                     /gene="rhaD"
                     /locus_tag="K0W68_001249"
     CDS             33170..33994
                     /gene="rhaD"
                     /locus_tag="K0W68_001249"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBI3072861.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            34004..34318
                     /gene="rhaM"
                     /locus_tag="K0W68_001250"
     CDS             34004..34318
                     /gene="rhaM"
                     /locus_tag="K0W68_001250"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBI3072862.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34619..35065
                     /locus_tag="K0W68_001251"
     CDS             34619..35065
                     /locus_tag="K0W68_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBI3072863.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            35076..36605
                     /locus_tag="K0W68_001252"
     CDS             35076..36605
                     /locus_tag="K0W68_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBI3072864.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVFLVCYLANDKALLGTQSGAGFLGAVVLGLAIGYFVFWFRKV
                     RLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAA
                     FLVGAMLAFDMGGPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAGLATFIAPK
                     LFTRQEKEAASSAIVVGATVATEPAIPYALAAPLPMITANTLAGGITGVLVIAFGIKR
                     LAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELVHE
                     H"
     gene            36595..37665
                     /locus_tag="K0W68_001253"
     CDS             36595..37665
                     /locus_tag="K0W68_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBI3072865.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37665..39413
                     /locus_tag="K0W68_001254"
     CDS             37665..39413
                     /locus_tag="K0W68_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HBI3072866.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39462..>39748)
                     /locus_tag="K0W68_001255"
     CDS             complement(39462..>39748)
                     /locus_tag="K0W68_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HBI3072867.1"
                     /translation="DVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQYQLETKK
                     YVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         9640 a        10326 c        10347 g         9435 t
ORIGIN      
        1 ccaccggcgc gggcgttttt gtccggtaac tgctgtaggt gcgggagagg atgaacggca
       61 gcggggcggg cagggcgatg tcggtttcac cgggaaggac cttcgcaccg agcagcggat
      121 tgaccggatg gccggacgtc accccgccgg ggcacaccga acaggccacg ccggtggggg
      181 caccgatgcg caccccggct gaaccctgga caatgctacc gccatactgc gtcatgtcgc
      241 cctgacgcgc cgccggtttt ccgctcatcg taaaatcctt ttgaaataaa taagaaccag
      301 aagcctagcg ccagtcaaca acaggaaatg ataatgagtt aacaccccgg aatttttcag
      361 aatttataaa aggcaaatta caggcacaaa aaaagcgccg tgcggcgctt ttttcggaaa
      421 tccggtctta tttgctgccc gggatgttga aacgcttgtt gaagcggtca acacggccac
      481 cggtagcaac atcacgctgt ttgccagtga agaacgggtg gcacttgctg cacacgtcga
      541 ggttcaggtc atgaccaacg gtggagcgga ttttcattac gttaccgcaa gagcagctag
      601 cagtaatttc ttcgtatttc gggtgaatat cttttttcat gggaaaacct cggtttaagg
      661 ccgcgtcgct cttccagccc taacgccaga caccacgcga tgttaaaagt atagcttcaa
      721 tacgatcatt tcgtacgaag gcgcgaaatc atacagaaat taaccagcgt atgcaaactg
      781 atccgcactc ttctacggca atgtgtatac taacccaccg aatttcaagt caggatgatg
      841 ctatgcccgt tgcccacgtt gccttgcccg ttccgcttcc tcgtaccttt gactatctgc
      901 tgccagaagg catggcggtt aaagccgggt gtcgcgtgcg cgtgccgttt ggcaaacagc
      961 aggagcgcat cggggttgtg gtatcagtta gcgatgtcag tgagctgccg cttaacgagc
     1021 tgaaagcggt agttgaagtg ctggacgttg agccggtatt tactcactcc gtctggcgat
     1081 tactgctctg ggcggcagat tactatcatc atccgattgg cgatgtgctg tttcatgcct
     1141 tgccgatttt actgcgccag gggcggcctg cggcgaacgc gccgatgtgg tactggtttg
     1201 ccactgaaca aggccaggcg gtggatctga acagcctgaa acgctcaccc aagcaacaac
     1261 aggcgctggc ggcgttacgg caagggaaaa tctggcgcga ccaggttgcc acgctcgaat
     1321 ttaatgatgc cgcgttgcag acgctgcgca aaaaaggtct gtgtgattta gcaagtgaaa
     1381 caccagagtt tagcgactgg cgaacgaact atgccgtttc tggtgagcgg ttgcgattga
     1441 ataccgaaca ggccaccgcc gttggcgcaa ttcatagcgc ggcagatact ttttctgcct
     1501 ggctgctggc gggcgttacc ggttccggta aaacggaggt ttatctcagt gtactggaaa
     1561 acgtgctcgc tcagggcaaa caggcgctgg tgatggtgcc ggaaatcggc ctgacaccgc
     1621 aaactatcgc ccgttttcgt gaacggttta atgcgcccgt ggaagttctg cattccggtc
     1681 tgaacgacag cgagcgtctt tcggcgtggc tgaaagcgaa aaatggcgag gcggcgattg
     1741 tgatcggcac ccgctccgcg ctgtttacgc cgtttaaaaa tctcggcgtg attgtcattg
     1801 acgaagagca cgacagttcc tacaagcagc aggaaggctg gcgctatcat gcccgcgacc
     1861 tggcggtgta tcgtgcgcat agcgagcaaa tcccgattat tcttggctcc gcaacgcccg
     1921 cgctggaaac gttgtgcaac gtccagcaga aaaaataccg cctgctgcgc ctgacgcgcc
     1981 gggcggggaa tgcgcgtccg gcaattcaac acgtgctgga tttaaaaggt cagaaggtgc
     2041 aggcaggtct ggctccggcg ttaatcaccc gtatgcgcca gcatttacag gccaacaatc
     2101 aggttatcct ctttcttaac cgccgtggct ttgcgcctgc gctactgtgc cacgactgcg
     2161 gctggattgc cgaatgccca cgttgcgatc actactacac gctgcatcag gcgcaacagc
     2221 atttgcgctg tcaccactgt gacagtcagc gtccggtgcc gcgccagtgc ccttcctgcg
     2281 gttccacgca cctggtaccc gtggggctgg gcaccgaaca gcttgaacag acgcttgcgc
     2341 cgctgttccc tgatgtgccc atttctcgta tcgaccgcga taccaccagc cgcaaagggg
     2401 cgctggaaca gcaactggcg gaagtacatc gcggcggtgc gaggattttg atcggcacac
     2461 aaatgctggc gaaaggtcac cactttccgg atgtgacgct ggtggcgtta ctggatgtgg
     2521 acggcgcgct gttttctgcc gattttcgct cggcagagcg tttcgctcag ctttacaccc
     2581 aggtcgccgg tcgtgccggg cgtgcgggta aacagggcga agtggtgctg caaacgcacc
     2641 atccggaaca tcctctgttg caaacgttgc tctataaagg ctacgacgcc tttgccgaac
     2701 aggcgctggc tgagcgacgc atgatgcagc tgccgccgtg gaccagccat gtgattgtgc
     2761 gtgcggaaga tcataacaat cagcatgcgc cgctgttcct gcaacaactg cgtaatctga
     2821 tcctctccag cccgctggca gacgacaaat tgtgggttct cggtccggtt ccggcactgg
     2881 cacctaaacg tggcggtcgc tggcgctggc agatattgtt gcagcaccct tcccgcgtgc
     2941 gcttgcaaca catcattaac ggtacgctgg cactcatcaa tacaataccg gattcccgta
     3001 aggtgaaatg ggtgctggat gttgatccga ttgagggtta aaccgctcac gatgcgaggc
     3061 ggatcgaaaa attcaatatt catcacactt ttcatgaaaa ttctgtaacc gttttcacgc
     3121 gctatctgct aaaaatgttg ccgatgtgaa gtaaacatgg atgtagtacg cctgacgtgc
     3181 caggcgagga gtgagtgtga aagcgaagaa gcaggaaact gccgcgacca tgaaagacgt
     3241 tgccctcaag gcaaaagtct ctacagcgac tgtctcccga gcattaatga atcccgataa
     3301 agtctcccag gccacccgta atcgggttga aaaagcggcc cgggaagtgg gttatttacc
     3361 gcagcctatg gggcgcaacg tcaagcgtaa tgaatcccgc accattctgg tgattgtccc
     3421 ggatatctgc gatcccttct ttagcgaaat tattcgcggt atcgaagtta cggcggcaaa
     3481 tcacggatat ctggtgctga ttggcgactg tgcgcatcaa aatcagcagg aaaaaacctt
     3541 tatcgatttg atcatcacca agcaaattga tggcatgttg ctgctgggtt caaggctgcc
     3601 gtttgatgcc agcattgagg aacagcgtaa tctgccgccg atggtgatgg cgaacgaatt
     3661 cgcaccggaa ctggagctac ctacagttca tatcgacaat ctgaccgccg catttgatgc
     3721 agtaaattat ctatatgagc aagggcataa acggattggc tgtatagccg gtcccgaaga
     3781 gatgccgctg tgtcactacc gcctgcaagg ttacgttcag gcgctgcgtc gctgcggcat
     3841 tatggttgat ccgcaataca tcgcccgtgg cgacttcacc ttcgaagccg gaagcaaagc
     3901 gatgcagcag ctgcttgatc ttccacaacc gcctactgct gtcttctgcc atagcgatgt
     3961 gatggcgctc ggcgcacttt ctcaggcaaa acgccagggg ctgaaagtcc cggaagacct
     4021 ttccataatc ggttttgata acatcgacct gacgcaattt tgtgatccgc cgctgacaac
     4081 catcgcgcag ccgcgttacg aaatcggtcg ggaagctatg ctgttattgc ttgatcaaat
     4141 gcaggggcaa cacgttggca gtggctctcg tttaatggac tgcgaactta tcatccgggg
     4201 atcaacacgc gcgttacctt aaagtaaacc tttaagactg ccgtgactgg tcaaaggccc
     4261 gccgcttaag taacatggcg ggctgacgaa cgaataaata cagcgaaacg atagtggcac
     4321 aacgagatta tgtacgccgc agccaaccgg caccttcgcg gcgaaaaaag agcacctcac
     4381 ggaaaaagca acgaaatctg cctgcggttt ctcccgctat ggtcgctatt gctgccgccg
     4441 tgcttgtgac ctttatcggt ggtctgtact tcattacgca tcacaagaaa gaagagtccg
     4501 agacgctgca aagccagaaa gtgaccggta acggactacc accaaaacca gaagaacgct
     4561 ggcgctacat taaagagctg gaaagtcgcc agccgggtgt gcgtgcgccc acagaacctt
     4621 ctgccggtgg tgaagtgaaa acgccggagc aactgacacc agaacaacgt cagcttcttg
     4681 aacaaatgca ggctgatatg cgccagcagc caacgcagct ggttgaagtt ccgtggaatg
     4741 aacagacgcc ggaacagcgt cagcaaacgc tacaacgcca gcgtcaggcg cagcaattag
     4801 cggagcagca acgcctggct cagcagtcgc gtacgactga acaaagctgg cagcagcaga
     4861 cgcgtacgtc gcaagccgcg ccggtacagg cgcaaccgcg ccagtctaaa ccggcttcta
     4921 ctcagcagcc gtaccaggat ctgctgcaaa cccctgcgca cacgaccgcg cagtcgaagc
     4981 cgcagcaagc tgcgccggtt gctcgtgccg ctgacgcgcc aaaaccgacg gcggagaaaa
     5041 aagacgaacg ccgctggatg gtgcagtgcg gttcgttcag aggcgcggaa caggcagaga
     5101 cggtacgtgc tcagctggcg ttcgaaggat ttgactcgaa aatcaccacc aacaatggct
     5161 ggaatcgtgt ggtcattggc ccggtgaaag gcaaagagaa cgcagacagc accctcaatc
     5221 ggttgaagat ggcgggtcat acaaactgca ttcggctcgc cgccgggggt tgaaaccctc
     5281 aaaatccccc ccatctataa ttgcattatg ccccgtactt ttgtacgggg tttgtactct
     5341 gtattcgtaa ccaaggggtc agctcgtgac aactatagta agcgtacgcc gtaacggcca
     5401 tgtggtcatc gctggtgatg gtcaggccac gttgggcaat accgtaatga aaggcaacgt
     5461 gaaaaaggtc cgccgtctgt acaacgacaa agtcatcgcg ggctttgcgg gcggtactgc
     5521 ggatgctttt acgctgttcg aactgtttga acgtaaactg gaaatgcatc agggccatct
     5581 ggtcaaagcc gccgttgagc tggcaaaaga ctggcgtacc gatcgcatgc tgcgcaaact
     5641 tgaagcactg ctggcagtcg cggatgaaac tgcatcgctt atcatcaccg gtaacggtga
     5701 cgtggtgcag ccagaaaacg atcttattgc tatcggctcc ggcggccctt acgcccaggc
     5761 tgcggcgcgc gcgctgttag aaaacactga acttagcgcc cgtgaaattg ctgaaaaggc
     5821 gttggatatt gcaggcgaca tttgcatcta taccaaccat ttccacacca tcgaagaatt
     5881 aagctacaaa gcgtaaggat ctcccatgtc tgaaatgacc ccacgcgaaa tcgtcagcga
     5941 actggataag cacatcatcg gccaggacaa cgccaagcgt tctgtggcga ttgctctgcg
     6001 taaccgctgg cgtcgcatgc agctcaacga agagctgcgc catgaagtga ccccgaaaaa
     6061 tatcctgatg atcggcccga ccggtgtcgg taaaactgaa atcgcccgtc gtctggctaa
     6121 gctggcgaat gcgccgttca tcaaagttga agcgaccaaa ttcaccgaag tgggctatgt
     6181 cggtaaggaa gtggattcta ttattcgcga tctgaccgat gccgccgtga aaatggtacg
     6241 cgtccaggct atcgagaaaa accgttatcg cgctgaagaa ctggcagaag aacgtattct
     6301 cgacgtgctg atcccacctg ctaaaaacaa ctggggacag accgaacagc agcaggaacc
     6361 gtccgctgct cgtcaggcat tccgcaaaaa actgcgtgaa ggccagcttg atgacaaaga
     6421 aatcgagatc gatcttgccg cagcaccgat gggcgttgaa attatggctc ctccaggcat
     6481 ggaagagatg accagccagc tgcagtccat gttccagaac ctgggcggcc agaagcaaaa
     6541 agcgcgtaag ctgaaaatca aagacgccat gaagctgctg attgaagaag aagcggcgaa
     6601 actggtgaac ccggaagagc tgaagcaaga cgctatcgac gctgttgagc agcacgggat
     6661 cgtgtttatc gacgaaatcg acaaaatctg taagcgcggc gagtcttccg gtccggatgt
     6721 ttctcgtgaa ggcgttcagc gtgacctgct gccgctggta gaaggttgca ccgtttccac
     6781 caaacacggg atggtcaaaa ctgaccacat tctgtttatc gcttctggcg cgttccagat
     6841 tgcgaaaccg tctgacctga tcccggaact gcaaggtcgg ctgccaatcc gcgttgaact
     6901 gcaggcactg accaccagcg acttcgagcg tattctgacc gagccgaatg cctctatcac
     6961 cgtgcagtac aaagcactga tggcgactga aggcgtaaat atcgagttta ccgactccgg
     7021 tattaagcgc atcgcggaag cggcatggca ggtgaacgaa tctaccgaaa acatcggtgc
     7081 tcgtcgttta cacactgttc tggagcgttt aatggaagag atttcctacg acgccagcga
     7141 tttaagcggt caaaatatca ctattgacgc agattatgtg agcaaacatc tggatgcgtt
     7201 ggtggcagat gaagatctga gccgttttat cctataatcg cgttcaatca ttttcatcat
     7261 tgtttgatgg ggctgaaagg ccccattttt attggcgcgt attatgactg aacaacaaat
     7321 tagccgaact caggcgtggc tggaaagttt acgacctaaa accctccccc tcgcctttgc
     7381 tgcaattatc gtcgggacgg cgctggcatg gtggcaaggt cacttcgatc cgctggtcgc
     7441 cctgctggta ctaattaccg ccgggctatt acagatcctt tctaacctcg ccaatgatta
     7501 cggcgatgcg gtaaaaggca gcgataaacc tgaccgcatt gggccgctac gcggcatgca
     7561 aaaaggggtc attacccagc aagagatgaa acgggcgctc attattaccg tcgtgctcat
     7621 ctgtctctcc gggctggcac tggttgcagt ggcatgccat acgctggccg attttgtcgg
     7681 tttcctgatt cttggcgggt tgtcgatcat tgccgctatc acctacaccg tgggcaatcg
     7741 tccttatggt tatatcggtc tgggtgatat ttccgtactg gttttctttg gctggttgag
     7801 cgtcatgggg agctggtatt tacaggctca tacattgatt ccggcactga tccttccggc
     7861 gaccgcgtgt ggcctgctgg caacggcagt actgaacatt aataacctgc gtgatatcaa
     7921 tagcgaccgc gaaaatggca aaaacacgct ggtggtgcgc ttaggtgaag tgaacgcgcg
     7981 tcgttatcat gcctgcctgc tgatgggctc tctggtatgt ctggcgctgt ttaatctctt
     8041 ttcgctgcat agcctgtggg gctggctgtt cctgctggcg gcaccattac tggtgaagca
     8101 agcccgttat gtgatgcggg aaatggaccc ggtggcgatg cgaccaatgc tggaacgtac
     8161 tgtcaaggga gcgttactga ctaacctgct gtttgtttta gggatattcc taagccagtg
     8221 ggcagcataa ctgacaaata tcaattaaca attgatgatt ttgccaacag cctacatagc
     8281 gcgatatact gaaaattctc gcagcaactg aatgttaagc ctatgaaata cgatacttcc
     8341 gagctttgtg acatctatca agaagatgtt aacgtcgtgg aaccgctgtt ctccaacttt
     8401 ggcggacggg cgtcgtttgg cggacaaata atcacggtaa aatgtttcga ggacaacggg
     8461 ttgctgtacg atctgctcga acagaatggc cgtggtcgtg ttcttgtcgt cgatggcggt
     8521 ggttctgttc gtcgcgcact ggtcgatgct gaactggcgc gtctggcagt acaaaatgaa
     8581 tgggaaggtc tggttattta cggcgcggtg cgtcaggtag atgacctaga agagttggat
     8641 atcggcatcc aggcgatggc ggcaattccg gttggtgccg ctggcgaagg cattggcgaa
     8701 agcgatgtcc gcgtcaattt tggcggtgta accttcttct ccggcgacca tctttatgcc
     8761 gacaataccg ggattattct ttcagaagat ccgctggata ttgaatgata agaaaggcac
     8821 cgcaaggtgc cttttttctg cgttacctgt tggcctacac agtaaagaaa ttacgcgaaa
     8881 gatgaagcgt aatcagacct cttccatgcg acccagcagg gcctgcagac gttcctgcca
     8941 gccgttctgc tgttctttca gatggttgtt ctcacgctcc agctcttcgc gctgatgctg
     9001 ggcattttga acttcctgcg acagtgagtt gtttttttct ttcagctctt cgatttccat
     9061 ctgcaacaga gtgatggtat caatcgcctg ctgtactttt gcttccagtt tctcaaacac
     9121 ttctaatgac attgtcatac ctctcctgaa ttgcaaggcg ttgatggata aaaaatcctc
     9181 gtcccgatta ccggtgacgc cttaataaat acgagcgcac tttagttagc tccgattgta
     9241 tgaagccgcg ccatcgctgt ccagcggcac gccttgcaga ttacggtttg ccacactttt
     9301 catccttctc ctggtgacat aatccacatc aatcgaaaat gttaataaat ttgttgcgcg
     9361 aatgatctaa caaacatgca tcatgtacaa tcagatggaa taaattgcgc gataacgctc
     9421 attttatgac gaagcacaca cattttaagt tcgatatttc tcgtttttgc tcgttaacga
     9481 taagtttaca gcatgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa
     9541 cattaactct tcaggatccg attatgagtc aaacatcaac cttgaaaggc cagtgcattg
     9601 ctgaattcct cggtaccggg ttgttgattt ttttcggtgt gggttgcgtt gcagcactaa
     9661 aagtcgctgg tgcgtctttt ggtcagtggg aaatcagtgt catttgggga ctgggggtgg
     9721 caatggccat ctacctgacc gcaggggttt ccggcgcgca tcttaatccc gctgttacca
     9781 ttgcattgtg gctgtttgcc tgtttcgaca agcgcaaagt tattcctttt atcgtttcac
     9841 aagttgccgg cgctttctgt gctgcggctt tagtttacgg gctttactac aatttatttt
     9901 tcgacttcga gcagactcat cacattgttc gcggcagtgt tgaaagtgtt gatctggcag
     9961 gcactttctc tacttaccct aatcctcata tcaattttgt gcaggctttc gcagttgaga
    10021 tggtgattac cgctattctg atggggctga tcctggcgtt aacggacgat ggcaacggtg
    10081 taccacgcgg ccctttggct cccttgctga ttggtctact gattgcggtc attggcgcat
    10141 ctatgggccc attgacgggt tttgccatga acccagcgcg tgacttcggt ccgaaagtct
    10201 ttgcctggct ggcgggctgg ggcaatgtcg cctttaccgg cggcagagac attccttact
    10261 tcctggtgcc gctttttggc cctatcgttg gcgcgattgt aggtgcattt gcctaccgca
    10321 aactgattgg tcgccatttg ccttgcgata tctgtgttgt ggaagaaaag gagaccacaa
    10381 ctccttcaga acaaaaagct tcgctgtaat atgactacgg gacaattaaa catgactgaa
    10441 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    10501 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    10561 ggctgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    10621 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    10681 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    10741 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    10801 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    10861 tggatcctcg accatgtgga aggctctcgc gagcgtgcgc gtcgtggtga attgctgttt
    10921 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    10981 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    11041 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    11101 gtgtacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    11161 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    11221 aacacctatg gcactggctg ctttatgctg atgaacaccg gcgagaaagc ggtgaaatca
    11281 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgctg
    11341 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    11401 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    11461 tatgtggttc cggcctttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    11521 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    11581 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    11641 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    11701 tccgatattc tcggcacccg cgttgagcgc ccggaagttc gcgaagtgac tgcattgggc
    11761 gcggcctatc ttgctggtct ggcggtcggt ttctggcaga acctcgacga gctgcaagag
    11821 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    11881 tacgcaggct ggaaaaaagc ggttaaacgc gcgatggcgt gggaagaaca cgacgtaatg
    11941 taaatgccgg atgaagcgtt tatgccgcat ccggtagtcc cgaaacgtgc gggggcaacc
    12001 ccgcacacat caataatccc tcccttcccc tgtgctacac ttcgcgccat tccttactgc
    12061 ttagagtttg ctatgagacg agaacttgcc atcgaatttt cccgcgtcac cgaatctgcg
    12121 gcgctggctg gctacaaatg gttaggacgc ggcgataaaa acaccgcgga cggcgcggcg
    12181 gtaaacgcca tgcgtattat gctcaaccag gtcaatattg acggcaccat cgtcattggt
    12241 gaaggtgaaa tcgacgaagc accgatgctc tacattggtg aaaaagtcgg tactggtcgc
    12301 ggcgacgcgg tagatattgc tgttgatccg attgaaggca cgcgcatgac ggcgatgggc
    12361 caggctaacg cgctggcggt gctggcagta ggcgataaag gctgcttcct caatgcgccg
    12421 gatatgtata tggagaagct gatcgtcgga ccgggagcca aaggcaccat tgatttgaac
    12481 ctgccgctgg cggataacct gcgcaatgtt gcggcggcgc tcggcaaacc gttgagcgaa
    12541 ctgacggtaa caattctggc taaaccacgc cacgatgccg ttatcgctga aatgcaacaa
    12601 ctcggcgtac gcgtatttgc tattccggat ggcgacgttg cggcctcaat tctcacctgt
    12661 atgccagaca gcgaagttga cgtgctgtac ggtattggtg gcgcgccgga aggcgtagtt
    12721 tctgcggcgg tgatccgcgc attagatggc gacatgaacg gtcgtctgct ggcgcgtcat
    12781 gacgtcaaag gcgacaacga agagaatcgt cgcattggcg agcaggagct ggcacgctgc
    12841 aaagcgatgg gcatcgaagc cggtaaagta ttgcgcctgg gcgatatggc gcgcagcgat
    12901 aacgtcatct tctctgccac cggtattacc aaaggcgatc tgctggaagg cattagccgc
    12961 aaaggcaata tcgcgactac cgaaacgctg ctgatccgcg gcaagtcacg caccattcgc
    13021 cgcattcagt ctatccacta tctggatcgc aaggacccgg aaatgcaggt gcacatcctc
    13081 tgattgattt gatcgattga gccttccagt ccttcgggac tggaattttt ttgttcggag
    13141 aacgaagata aggcaagtca atcaaaacag gagaaaaaca tggctgattg ggtaacaggc
    13201 aaagtcacta aagtgcagaa ctggaccgac gccctgttta gtctcaccgt tcacgccccc
    13261 gtgcttccgt ttaccgccgg gcaattcacc aagcttggcc ttgaaatcga cggcgaacgc
    13321 gtccagcgcg cctactccta tgttaactcg cccgataatc ccgatctgga gttttacctg
    13381 gtcaccgtcc ccgatggcaa attaagccca cgactggcgg cactgaaacc aggcgatgaa
    13441 gtgcaggtgg ttagcgaagc agctggcttc tttgttctgg aggaagtacc agattgcgaa
    13501 acgctatgga tgctggcaac cggtacagcg attggccctt atttatcgat tctgcaacta
    13561 ggcaaagatt tagatcgctt caaaaatctg gtcctggttc acgccgcacg ttatgccgcc
    13621 gacttaagct atttgccact gatgcaagaa ctggaaaaac gctacgaagg caaactgcgc
    13681 attcagacgg tggtcagtcg ggaaacggca gcggggtcgc tcaccggacg gataccggca
    13741 ttaattgaaa gtggggaact ggaaagcgcg attggcctgc cgatgaataa agaaaccagc
    13801 catgtgatgc tgtgcggcaa tccacagatg gtgcgcgata cacaacagtt gctgaaagag
    13861 acccggcaga tgacgaaaca tttacgtcgc cgaccgggcc atatgacagc ggagcattac
    13921 tggtaagcgg ttacttatcg ataaacggca cgatgagcaa atccgcactc atcttattga
    13981 tcatcccgcg atatgccggc atcaaacggt tgataaatga gtggtgatga ccacagacaa
    14041 ggaggtcgca ctgctctttt tgcataattt ccagcagtgt ttccggcatt tctccgcgtt
    14101 caatacgcag ttttgtcttc ggccattgaa tatttttcgt cagtttatac agcttgttat
    14161 ccgacttatt cttcaacaat tgaagaatat cttctgttgc agggaagtag atacccggat
    14221 acaactcgct taagccatca tcaatatgaa ttaacgtcag gtgagcgtca ttatgtctgg
    14281 cgagctccag ggctttattc accagtaagg catcttcttc attcccggaa attgccacgc
    14341 caatgtgttt ataagccatg tttaactcct tctaaagcaa ctccatcaag ctaacgaacg
    14401 cagtgataac tcaaaaataa tcatctcggc ctggcatgag aaagtgaagg ccgcatcaag
    14461 ctcaacttgc ccctccagat ccttaatctt ataggccacg ctggcccctt catctgcaga
    14521 ggatgcagcg gcggcaagct ctttcagttt ggtcataaaa gactctgctt cttcgcgggt
    14581 ggcaaaaaag cgcgaaaact tactggtaca gttgtcgtta tcaatcaccg taccgatatc
    14641 aatcgcacat cctttagtac tgcatttatc tacgacatct ttcatagggc acctctgtat
    14701 attcgcctct ctgtcgatta cgacttaaga ataatgcgcc gttaagggaa gaacaaacaa
    14761 gtaatgatct tatggattac catttttttt gatattgctt aaggtgaaga gttcagaagg
    14821 attttccggg tattgtcatt tgcatcgtgg aaataacaat cccggaaaaa aatagcagta
    14881 agagatatat taaaggttaa ttacttgatt tattgtcggc tttatacttc acatcctgag
    14941 tatctttacc atatttattt tcgccttgtg tgccaacaaa cgcaccaaga tcgataagca
    15001 tcatcaccag aatcaacgtc gggacaaaac gccccaccgc ccattgccag acacccggta
    15061 aaatcgccca gttacccgcc agcagcatcc acgccacaat catcagaaat gcccatgcgc
    15121 cggaacgccc gcgatcatgc aagcgcttaa cagttactgc cgctgttggc caaagcaagc
    15181 acacaaggca aaacgccgcg gtctgaatat cgagtaaatt tttacccgcc agtgagaaaa
    15241 gcaccagcat gcctgcgaac cacaggccta tccaaatcca gaaatcacgg cgtccaatac
    15301 gccctttaaa tgagaataac cattgctgta tggtcatgta agttccttga tggttgtctt
    15361 ttccaggatt ctaccctttt gacaagggcg acagatatcg ttttaatcgg agccagtcat
    15421 aacaaaaggt actgtcaatg aagccagggt gtacgctgtt ttttctctta tgttctgcat
    15481 taaccgttac aacaacggcg catgcacaaa caccagatac ggcaacgacc gcgccttatc
    15541 tgctggctgg agcccctact ttcgatctct ccatcagcca gtttcgagaa gactttaaca
    15601 gccagaatcc tagcctgcca ctgaacgaat ttcgtgccat cgacagcagt cccgacaaag
    15661 ccaatctcac tcgtgctgca agtaaaatta acgagaactt gtatgcttct acagcgctgg
    15721 agcgcggtac cttaaaaatc aaaagcattc aaatgacctg gctacccatc caggggccag
    15781 agcaaaaagc agcgaaagcg aaagctcagg aatacatggc agcagtgatc cgcacactca
    15841 ccccattaat gaccaaaaca caaagccaga aaaaactgca gtcgctacta acggcgggga
    15901 aaaacaaacg ttattacacc gagacagaag gtgcactgcg ttatgttgtc gcggacaaca
    15961 gcgaaaaggg gctgaccttc gctgttgaac cgattaagct ggcgctatct gaatcgcttg
    16021 aaggtttgaa taaatgacaa aaagcaaagc ctttgtgccg atgaatctct atactgtttc
    16081 acagacctgc tgccctgcgg ggcggccatc ttcctttatt cgcttataag cgtggagaat
    16141 taaaatgcga catcctttag tgatgggtaa ctggaaactg aacggcagcc gccacatggt
    16201 tcacgagctg gtttctaacc tgcgtaaaga gctggcaggt gttgctggct gtgcggttgc
    16261 aatcgcacca ccggaaatgt acatcgatat ggcgaagcgc gaagctgaag gcagccacat
    16321 catgctgggt gcgcaaaacg tggacctgaa cctgtccggc gcattcaccg gtgaaacttc
    16381 tgccgctatg ctgaaagaca tcggcgctca gtacatcatc atcggccact ctgaacgtcg
    16441 tacttaccac aaagagtctg acgaactgat cgcgaaaaaa ttcgcggtgc tgaaagagca
    16501 gggcctgact ccggttctgt gcatcggtga aaccgaagct gaaaacgaag cgggcaaaac
    16561 tgaagaagtt tgcgcacgtc agatcgacgc ggtactgaaa actcagggtg ctgcggcatt
    16621 cgaaggtgcg gttatcgctt acgaacctgt atgggcaatc ggtactggca aatctgcaac
    16681 tccggctcag gcacaggctg ttcacaaatt catccgtgac cacatcgcta aagttgacgc
    16741 taacatcgct gaacaagtga tcattcagta cggcggctct gtaaacgcgt ctaacgctgc
    16801 agaactgttt gctcagccag atatcgacgg cgcgctggtt ggcggtgctt ctctgaaagc
    16861 tgacgctttc gcagtgatcg ttaaagctgc agaagcggct aaacaggctt aagtctgaca
    16921 ggtgccggat ttcatatccg gcacttactt tccttaactc ttcgccttaa cgcaaaatct
    16981 cacactgatg atcctgaatt tcctcggctg aagcacggtt aagcgtcagt agatttcgtt
    17041 gtgtcgccag caatacaaat gagttatcac tctgccgtac catcgccagc ccgtagcttc
    17101 ccatatgttc ccgcgcctca ggtacttctt ctgccagcat cataaatggg ctgcgttgta
    17161 ccagttcgct ttccgttacc cgacgcgcca ggtattcatg cccacgcaaa ccacctggca
    17221 gtggcaacca gcggctgctg atgttcgcca gattgttatc cagttgttcg cgcacatcag
    17281 gacgaataca agagatatga atatgaaaat ggttttgcgt acgcccggtg cgggagttga
    17341 tcgccaaaga aaccgcgcga tcgggaaccg gctggccgta ttttttgctc ataaaatcac
    17401 gcgcctgcca ggccaaccaa aagaagttcg gcgttgaagg atcggtcaac aaaggacttt
    17461 cagtaccgtt aatacgatac gttggcatca acagatattg cagtggacca ttaagatctt
    17521 ttaaaaccac gtatccggca ttgggtttga cttccgcaca tggcgaagga ttttgatttt
    17581 gctgctgatt gggcaaacat tcctcaagga caatcttacg taatgtatcc gactcttcac
    17641 cggttaattt ccagtaacca ataccggcag ccacaacggc gataactatc atcaccaaaa
    17701 aaagaagacc cgcttttttc atcttttttt ccctgtacct caaagagatg caagggtaac
    17761 gcaaaatcgt gacaaataaa aaacccggtc gcgagaaacc accgggtttg ataattatcc
    17821 tggggaaatc agcgtttgct gatctgatcg aacgtaccgc cgttagcaaa atgctctttt
    17881 tgcgctttcg tccagccgcc gaactcttca tcaatggtga ataacttcag ctttggaaac
    17941 gcattttcgt actttttcgc tacttcagcg tcgcgcggac ggtagtagtt tttcgcggca
    18001 atttcctgac cttctggcga gtagagatat ttcaggtagg cttccgccac ctctttggtg
    18061 ccttttttct cgaccacttt atcgaccacc gacacggttg gctctgcgag gatagactca
    18121 ctcggcgtga cgatttcgaa tttatctttc cccagttcat tcgctgccag cagagcttcg
    18181 ttttcccagg cgatcagtac atcgccaatc ccgcgctcga caaaggtgtt agtggagccg
    18241 cgcgcgccag aatccagaac ttcgacgttt ttatacagtg cccgaacaaa atcctgtgct
    18301 tttgcctgat cgttgttgtt gtgatgcagc gcgtagcccc aggcagctag gtagttccag
    18361 cgtgcgccac cagagctttt cggattaggc gtgatcaccg aaacacccgg tttaatcaga
    18421 tcgttccagt catggatctg cttcggattg cccttacgca ccaggaaaac aatggtggaa
    18481 gtgtacggtg cggagttatc cggcagacgt ttgatccact ctttatcaat ccgcccacgt
    18541 tccgcaatcg cgtccacgtc ataggccaga gccagcgtga caacatcagc ttcaataccg
    18601 ttgattaccg acgtcgcttg tttacctgaa ccaccgtgtg actgacgaat caccacgtta
    18661 tcaccagttt gctgtttcca gtgggcgctg aatgccttgt tgtactgttc gtacaattcg
    18721 cgcgttggat catatgaaac gttaagaagc tgaatatcct ttgccataac gctggttgcc
    18781 gccagcaaaa atgttaaccc tacgccccac ttgttcatcg cccgactctc ttatgttgtg
    18841 ttgtgatgag caaagcgtgc cagaaggtta accaaacatt aaagaataaa aaaagattgg
    18901 ctataacttg cgggtatatg ttgagggatt aaaaaggcgg aagaaatccg cctcatattg
    18961 ctgacaaagt gcgctttgtc catgccggat gcggcgcgaa cgccttatcc ggcctacaaa
    19021 agtttgcaaa ttcaataaat tgcagaattc atgtaggcct gataagcgaa gcgcatcagg
    19081 catttttgct tctgtcatcg gtttcaggct aaaggaatct gcctttttcc gaaatcatta
    19141 atacagtttt ttcgcgcagt ccagccagtc acctttgaac ggacgcttca tgttttcgat
    19201 agcgtcgatg atgtcgtggt gaaccagctg ttcgttctgg atacctacac aacgaccgcc
    19261 gtaacctgcc agcagcagat cgatagcgta agcgcccata cgggaagcca gaatacggtc
    19321 gtaaggcacc ggagaaccac cgcgctggat gtggcccagc acagttgcgc gggtttcacg
    19381 accggtttct ttctcgatga aatgcgccag ttcgtcaaca tcacacatat gttcggtaat
    19441 cgccacgatc gcgtgttttt tacctttcgc gatacccgct ttgatttcgt ttaccaggtc
    19501 ttcacggctg aattcaactt ccggaaccac aacgaattca cagccaccgg caatggccgc
    19561 agccaacgtc agatcgccac aataacggcc catcacttcc accacggaaa tacgctggtg
    19621 agaagaagag gtgtcacgca gacggtcgat cgcttctaca acggtgctca gcgcagtgaa
    19681 gaaaccgata gtgtagtcag tgcctttgat gtcgttgtcg atagtgcccg gcagaccgat
    19741 gcacgggaag cccatttcgg tcagacgcat tgcacccatg taggaaccgt caccgccgat
    19801 aaccaccagc gcgtcgatac cacgtttttt caggttttcg atagccacgg cgcggatgtt
    19861 ctcgtcgcgg aattccggga agcgcgcaga accgaggaac gtaccgccac ggttgatcat
    19921 gtcagaaacg ctgtaacggt ctagctgtac catacggtct tcatacagac ccagatagcc
    19981 gtcataaatg cccattactt ccagaccttc tgtcagcgca gaacgaacaa ccccgcgaat
    20041 tgcggcgttc atgcctggcg catcaccgcc gcttgtcaac acaccgattt tcttaatcat
    20101 gactacctct gaactttgga atgcaaaata aaatctgttg ccggaagtct tcttgcacat
    20161 cgaagtgatc caacgaatgt gcaaatagta taacaatcac ttcctgctga attgattcag
    20221 gtcaggccaa atggcggtat tttatacaca aaatgcgggt ctggctctct tttatactga
    20281 ttatgaaagc atagaccgtt taccctccct gggtacgacg gaacaggggt cctgatggat
    20341 aattacatcc gatcccggaa aacgccgtaa aatagcctgc tctacctgat ccgccaccat
    20401 atgtgcctga accaaaggca gagagtcttc catttccaaa tgaatctgaa taaagcgggt
    20461 cggccctgac tgccgcgtgc gaagatcgtg agcgccgcta acacccggcc aggaagtcac
    20521 gatatcaata atttcttgcc gttcctcatc aggcaatgcg cgatccagta atgactgtac
    20581 cgcctcatat cccatgcgta acgcgctata taaaatatag atgccgattc ccaatgcaaa
    20641 cagagcatcg gcgcgatgcc agccgtacca ggacaacccc agcgccagca gaattgcgcc
    20701 gttcatcata acatcagact ggtaatgtag catatcagcc cgcaccgcct ggctttgcgt
    20761 ccggcgcacc acccaccgct gaaacgagac aaggataatc gtacaaatta gcgccacaat
    20821 tgtcacgata accccgacgc ctggatctgt catcggtgtt ggagatacca gatgttgaat
    20881 acccgtcaaa aacaggaata gtgccgaacc ggagataaac atactttgcg ccagcgccgc
    20941 gagggactca gctttaccgt gaccaaacga gtgattatcg tcggcaggtt gcagggaata
    21001 tcgcaccacc agtaaattcg tcaacgacgc gccgatatcc accagcgaat ccaccagcgc
    21061 ggcgagaata ctcaccgacc cggtatacca ccatgcaaaa attttaatca gcaatagcag
    21121 cgaagccatc gccgtcgcag caatcgccgc ccgactgacc agccgtccat aagattgatt
    21181 cataaatact cccactatca actgacgcta gtataacgga agcaaatcat ctgcaatgca
    21241 ttaagcagca ggcaaattga ggataaaaaa aaccccacat catgtggggg aagacaggga
    21301 tggtgtctat ggcaaggaaa acagggttta ctactgggaa cgtgagttgc tactactcaa
    21361 tagcttcaac gatgaacttt tttgccattg cgtcacgtca cgcaactgct ccattcgttg
    21421 ttgatgtttc tcgtttaaaa ccgcttgctg ctccggcgtt aacaggcgat acatttggtt
    21481 gcggactttt gccatctcaa cctgacgagc aatttgctca ttcgccattt tttctgcctg
    21541 tgcgcgcaca gcgttttcat caaaattttc tgcggtgaca aggcgatgca ttgtctccag
    21601 ttcgctaaca ttaacaggag gctgttcgtg ccgggcctgt tgcataagat ctcgcatctg
    21661 ctgacgctga tgttcggtta aacttatgcc gtcgaacata tggctctgcg tactgcgctg
    21721 cgtaagttct tcacccggat gccagttatc gcctgaaccg acttcagcag cgtggcttaa
    21781 tgaactgact gccagcgttg aggccatgac ggcagcggta actatgcgca tcatttgctc
    21841 ccaaaatctt tctgtcgcga ttcaacgata gagagtttac gattcaggct gcaaacatgc
    21901 gtcagggggt gtaaaacaac gtaaagtcat ggattagcga cgtctgatga cgtaatttct
    21961 gcctcggagg tatttaaaca atgaataaaa tcctgttagt tgatgatgac cgagagctga
    22021 cttccctatt aaaggagctg ctcgagatgg aaggcttcaa cgtgattgtt gcccacgatg
    22081 gggaacaggc gcttgatctt ctggacgaca gcattgattt acttttgctt gacgtaatga
    22141 tgccgaagaa aaatggtatc gacacattaa aagcacttcg ccagacacac cagacgcctg
    22201 tcattatgtt gacggcgcgc ggcagtgaac ttgatcgcgt tctcggcctt gagctgggcg
    22261 cagatgacta tctcccgaaa ccgtttaatg atcgtgagct ggtggcacgt attcgcgcga
    22321 tcctgcgccg ttcgcactgg agcgagcaac agcaaaacaa cgacaacggt tcaccgacac
    22381 tggaagttga tgccttagtg ctgaatccag gccgtcagga agccagcttc gacgggcaaa
    22441 cgctggagtt aaccggtact gagtttaccc tgctctattt gctggcacag catctgggtc
    22501 aggtggtttc ccgtgaacat ttaagccagg aagtgttggg caaacgcctg acgcctttcg
    22561 accgcgctat tgatatgcac atttccaacc tgcgtcgtaa actgccggat cgtaaagatg
    22621 gtcacccgtg gtttaaaacc ttgcgtggtc gcggctatct gatggtttct gcttcatgat
    22681 aggcagctta accgcgcgca tcttcgccat cttctggctg acgctggcgc tggtgttgat
    22741 gttggttttg atgttaccca agctcgattc acgccagatg accgagcttc tggatagcga
    22801 acagcgtcag gggctgatga ttgagcagca tgtcgaagcg gagctggcga acgatccgcc
    22861 caacgattta atgtggtggc ggcgtctgtt ccgggcgatt gataagtggg caccgccagg
    22921 acagcgtttg ttattggtga ccaccgaagg ccgcgtgatc ggcgctgaac gcagcgaaat
    22981 gcagatcatt cgtaacttta ttggtcaggc cgataacgcc gatcatccgc agaagaaaaa
    23041 gtatggccgc gtggaactgg tcggtccgtt ctccgtgcgt gatggcgaag ataattacca
    23101 actttatctg attcgtccgg ccagcagttc tcaatccgat ttcattaact tactgtttga
    23161 ccgcccgcta ttactgctga ttgtcaccat gttggtcagt acgccgctgc tgttgtggtt
    23221 ggcctggagt ctggcaaaac cggcgcgtaa gctgaaaaac gctgccgatg aagttgccca
    23281 gggaaactta cgccagcacc cggaactgga agcggggcca caggaattcc ttgccgcagg
    23341 tgccagtttt aaccagatgg tcaccgcgct ggagcgcatg atgacctctc agcagcgtct
    23401 gctttctgat atctctcacg agctgcgcac cccgctgacg cgtctgcaac tgggtacggc
    23461 gttactgcgc cgtcgtagcg gtgaaagcaa ggaactcgag cgtattgaaa ccgaagcgca
    23521 acgtctggac agcatgatca acgatctgtt ggtgatgtca cgtaatcagc aaaaaaacgc
    23581 gctggttagc gaaaccatca aagccaacca gttgtggagt gaagtgctgg ataacgcggc
    23641 gttcgaagcc gagcaaatgg gcaagtcgtt gacagttaac ttcccgcctg ggccgtggcc
    23701 gctgtacggc aatccgaacg ccctggaaag tgcgctggaa aacattgttc gtaatgctct
    23761 gcgttattcc catacgaaga ttgaagtggg ctttgcggta gataaagacg gtatcaccat
    23821 tacggtggac gacgatggtc ctggcgttag cccggaagat cgcgaacaga ttttccgtcc
    23881 gttctatcgg accgatgaag cacgcgatcg tgaatctggc ggtacaggat tggggctggc
    23941 gattgttgaa accgccattc agcagcatcg tggctgggtg aaggcagaag acagcccgct
    24001 gggcggttta cggctggtga tttggttgcc gctgtataag cggagttaaa ctccgcattt
    24061 gtaggcagga taaggcgttt acgccgcatc cggcatttga gcaggatgcc tgatgcgacg
    24121 ctgatagcgt cttatcaggc ctacactccg ggggttattt tccccacaac cggcgggaaa
    24181 aatcttcgat cttgccgctc agtcggcgct tttgcatcgt ccgcgtccag cttttcgata
    24241 acccggcggc ggagagtaaa cggtgatact gatcgtcatc aaacggcata tgccatgcga
    24301 tggcggcagc ctcctgcacg gtcacatcgc tgacacggga aaccaactcc agcggcgcgt
    24361 ccgcagacac tttccctggc gctatcacgc tatacaacca ccccacctta cctgtgtttt
    24421 gcatcaactg cgctatatcg ctgatatcaa aatggtaatt gagtttatag caaggcgagc
    24481 gcggctggct gacctgaatt aatgcctctc cccagcggaa aatatcgccc atatgaacat
    24541 tactttccgt gagaccgtcg gttgagagat tttcgccaaa cgcaggcgca acaaacaact
    24601 ccgcctgttc cggaaattcc cgcacccagt agagatagtg ctcacgagga taatgacaca
    24661 gcgctctgtc tggcccgccg tgaacctttt tctccgcctg ctcgtcacct tccagcccca
    24721 gctccgtcag catcaactca ccatcaacct ggattttggc aattgcgctg ggtttgcctt
    24781 cgggataagc ctgaatcttg cctgtgtata catcaaccgg atatcgcatc tgttccctct
    24841 ccacgagaaa tttccccatt agaccatatt tattttatgt caggcgtgct gttttcgcgg
    24901 taagtatcca ggcaggaact caatagcaaa ccggacgaac gttagttcat ccggctgtgg
    24961 ggattaagga agcaggttta tttcaccgta cctaaaattt cgattttcgc tgctctggct
    25021 ttgactttac gtgaccagat agaggtaaga attggcacca gaatcgaagt cacaatgacc
    25081 gccgtcgcta ccagcgaagt tgctgccgga gccatcggtt taaacgcagg caccatttct
    25141 gcaatcagca caggtgtcgc taccgcggcc cctgcggaac tggaagcggc aattccggca
    25201 gtgccatcgc caccgccaat caatttatcg gcgataatca acggaatacc ggtcacgata
    25261 attactgcca cacccaacag gatccccagc aaaccagtct gggcaatcac agtcaaatca
    25321 atggtattgc ccagcgcgaa ggcaaagaat ggaatcagcg tttgcactgc tttgctgaaa
    25381 aattctcgta attcagggtc aagattcccc agggcaaagc ccaccaggaa cggtaatact
    25441 gcgccgacga aaacatgcgg ttcaaacgag gcaatcccgg cagtgcccag aataatcatc
    25501 gtcatgagcg gcccggactc caacgacatc aacacaaatg ccccagcttc ttctttcgta
    25561 ccgtactgct gcatgatgga agcgtaaagt ccgccgttgg tcatatccat cgccgccacc
    25621 agcgccagcg ttgaaagtcc ggcaaagaat ccaacttcaa caccatgttc cggaataatg
    25681 cgcgaggcaa ttgccgcaac cacccacgcg acggcaattt tagttaccac cagcgtaccg
    25741 gatttacgca gtaccgttcc cgtcgcgctt aattttattg acgcccccat gcaaaaaaac
    25801 cacaccgcca gaatgggcac cgtaccggta atcatcccgt tggtgaatga tccaaaatat
    25861 ttccccgccc ccggcgagaa ggtgtggcac agtgcgccaa ggaatagcgg gacgagcatc
    25921 atcccccccg ggattttctc aatcgagcgt tttatctgca tttccatcac ctgtcggcat
    25981 atttttaagg gctgatgcgt aattcctcaa tggagcatca gtgttgatgg aaacgaagat
    26041 agacattcat ttaatcaata aaagtgatca aaaccacatc aattgaaacg ctgttttatt
    26101 tttatcggat cattgttttt attttgaaat tgatcacaaa aaaaccaccg ccgttggcga
    26161 tggttcattt tttaagctga tatgcggcct atacgcctca ttgcagcagg cggcaaatga
    26221 ttattttttc gccgcaaaac gtgccgctgc ttcgtcccag ttcaccacgt tccagaactc
    26281 tttaatgtag tccggacggc ggttctggaa tttcaggtag taagcatgtt cccacacatc
    26341 caggcccaga atcgggaagc cggaagcgcc agaaatagct tcacccatca gcggagaatc
    26401 ctggttagca gtagaaacca ccgccagttt atcgcctttc agcaccagcc atgcccagcc
    26461 ggaaccaaag cgggaagctg ccgctttttc aaattctgct ttgaagttat caacggagcc
    26521 gaagtcacgt tcgatagccg ctttcaggtc accctgcagg gtggtgcctt ttttcagacc
    26581 tttccagaac aggctgtggt tagcgtgacc gccagcgttg ttgcgcagta cggttttctt
    26641 gtctgctggc agctggtcca gtttggtgat cagctcttca accggcaggt tggcaaattc
    26701 tggcaggctt tccagcgccg cgttggcgtt gtttacgtag gtctgatggt gtttggtgtg
    26761 gtggatttcc atggtctgct tatcgaagtg cggttccagg gcatcgtaag catacggcag
    26821 ggatggcagg gtatagctca tattcatctc cagtattgtc gggcggccga ttgttaatgc
    26881 cgcgtaagca gttggttcat tatagttaat taaatgatat tgaaaatgat tatcaatgcc
    26941 gtacttttcg taagggtatg gttttgcagg aaaatgcccg agatgtgaag caaatcaccc
    27001 acttaatgcc gtgattgcca gtaaatcgac aacggcggca acaggcgaaa ggttaatcga
    27061 cagcacgatt tttacactca tctcgtcgga gatgtgaagc gacgaaaaat gatgaggata
    27121 agaagatgag taacgcgatt acgatgggga tattttggca tttgatcggc gcggccagtg
    27181 cagcctgttt ttacgctccg ttcaaaaaag taaaaaaatg gtcatgggaa accatgtggt
    27241 cagtcggtgg gattgtttcg tggattattc tgccgtggac catcagcgcc ctgttactac
    27301 cggatttctg ggcgtattac agctcgttta gtctctctac gctgctgcct gtttttctgt
    27361 tcggcgctat gtgggggatc ggtaatatca actatggcct gaccatgcgt tatctcggca
    27421 tgtcgatggg aattggcatc gccattggca ttacgttgat tgtcggtacg ctgatgacgc
    27481 caattatcaa cggcaatttc gatgtgctga ttaacaccga aggcggacgc atgacgttgc
    27541 tcggcgttct ggtggcgctg attggcgtag ggattgtaac tcgagccggg cagttgaaag
    27601 agcgcaagat gggcattaaa gccgaagagt tcaatctgaa aaaagggctg gtgctggcgg
    27661 tgatgtgcgg cattttctct gccgggatgt cctttgcgat gaacgccgca aaaccgatgc
    27721 atgaagccgc tgccgcactt ggcgtcgatc cactgtatgt cgctctgcca agctatgttg
    27781 tcatcatggg cggcggcgcg atcattaacc tcggtttctg ttttattcgt ctggcaaaag
    27841 tgaaggattt gtcgctaaaa gccgacttct cgctggcaaa accgctgatc attcacaatg
    27901 tgttactctc ggcactgggc gggttgatgt ggtatctgca attctttttc tatgcctggg
    27961 gccacgcccg cattccggcg cagtatgact acatcagttg gatgctgcat atgagtttct
    28021 atgtattgtg cggcggtatc gtcgggctgg tgctgaaaga gtggaacaat acaggacgcc
    28081 gtccggtaac ggtgttgagc ctcggttgtg tggtgattat tgtcgccgct aacatcgtcg
    28141 gcatcggcat ggcgaattaa tctttctgcg aattgagatg acgccactgg ctgggcgtca
    28201 tcccggtttc ccgggtaaac accaccgaaa aatagttact atcttcaaag ccacattcgg
    28261 tcgaaatatc actgattaac aggcggctat gctggagaag atattgcgca tgacacactc
    28321 tgacctgtcg cagatattga ttgatggtca ttccagtctg ctggcgaaat tgctgacgca
    28381 aaacgcgctc actgcacgat gcctcatcac aaaatttatc cagcgcaaag ggacttttca
    28441 ggctagccgc caaccgggta atcagcttat ccagcaacgt ttcgctggat gttggcggca
    28501 acgaatcact ggtgtaacga tggcgattca gcaacatcac caactgcccg aacagcaact
    28561 cagccatttc gttagcaaac gacacatgct gactactttc atgctcaagc tgaccgataa
    28621 cctgccgcgc ctgcgccatc cccacgctac ctaagcgcca gtgtggttgc cctgcgctgg
    28681 cgctaaatcc cggaatcgct ccctgtcagt caagattcag cttcagccgc tccgggcaat
    28741 aaataatatt ctgcaaaacc agatcgttaa cggaagcgta ggagtgttta tcgtcagcat
    28801 gaatataaaa gagatcgcca cgggtaatgc gataaggacg atcgttgagt acatgcagac
    28861 cattaccgcg ccagacaatc accagctcac aaaaatcatg tgtatgttcg gcaaagacat
    28921 cttgcggata acggtcagcc acagcgactg cctgctggtc gctggcaaaa aaatcatctt
    28981 tgagaagttt taactgatgc gccaccgtgg ctacctcggc cagagaacga agttgattat
    29041 tcgcaatatg gcgtacaaat acgttgagaa gattcgcgtt attgcagaaa gccatcccgt
    29101 ccctggcgaa tatcacgcgg tgaccagtta aactctcggc gaaaaagcgt cgaaaagtgg
    29161 ttactgtcgc tgaatccaca gcgataggcg atgtcagtaa cgctggcctc gctgtggcgt
    29221 agcagatgtc gggctttcat cagtcgcagg cggttcaggt atcgctgagg cgtcagtccc
    29281 gtttgctgct taagctgccg atgtagcgta cgcagtgaaa gagaaaattg atccgccacg
    29341 gcatcccaat tcacctcatc ggcaaaatgg tcctccagcc aggccagaag caagttgaga
    29401 cgtgatgcgc tgttttccag gttctcctgc aaactgcttt tacgcagcaa gagcagtaat
    29461 tgcataaaca agatctcgcg actggcggtc gagggtaaat cattttcccc ttcctgctgt
    29521 tccatctgtg caaccagctg tcgcacctgc tgcaatacgc tgtggttaac gcgccagtga
    29581 gacggatact gcccatccag ctcttgtggc agcaactgat tcagcccggc gagaaactga
    29641 aatcgatccg gcgagcgata cagcacattg gtcagacaca gattatcggt atgttcatac
    29701 agatgccgat catgagcgcg tacgaaacag accgtgccac cggtgatggt atagggctgc
    29761 ccattaaaca catgaatacc cgtgccatgt tcgacaatca caatttcatg aaaatcatga
    29821 tgatgttcag gaaaatccgc ctgcgggagc cggggttcta tcgccacgga cgcgttacca
    29881 gacggaaaaa aatccacact atgtaatacg gtcatactgg cctcctgatg tcgtcaacac
    29941 ggcgaaatag taatcacgag gtcaggttct taccttaaat tttcgacgga aaaccacgta
    30001 aaaaacgtcg atttttcaag atacagcgtg aattttcagg aaatgcggtg agcatcacat
    30061 caccacaatg aagctaattg tgaacatcat cacgttcatc tttccctggt tgccaatggc
    30121 ccattttcct gtcagtaacg agaaggtcac gaattcaggc gctttttaga ctggtcgtaa
    30181 tgaaattcag caggatcaca ttatgacctt tcgcaattgt gtcgccgtcg atctcggcgc
    30241 atccagtggg cgcgtgatgc tggcgcgtta cgagcgtgaa tgccgcagcc tgacgctgcg
    30301 cgaaatccat cgttttaaca atggcctgca tagccagaac ggttatgtca cctggaatgt
    30361 ggatagcctg gaaagtgcca ttcgccttgg attaaacaag gtgtgcgagg aagggattcg
    30421 tatcgatagc attgggattg atacctgggg cgtggacttt gtgctgctcg accaacaggg
    30481 tcagcgtgtg ggcctgcccg ttgcttatcg cgatagccgc tccaatggcc taatggcgca
    30541 ggcacagcaa caactcggca aacgcgatat ttatcaacgt agcggcatcc agtttctgcc
    30601 cttcaatacg ctttatcaac tgcgtgcgct gacggagcaa caacctgaac ttattccaca
    30661 cattgctcac gctctgctga tgccggatta cttcagctat cgcctgaccg gcaagatgaa
    30721 ctgggagtac accaacgcca cgaccacgca actggtcaat atcaatagcg acgactggga
    30781 cgagtcgcta ctggcgtgga gcggggctaa caaagcctgg tttggtcgcc cgacgcatcc
    30841 gggtaatgtc attgggcact ggatttgccc gcagggtaat gagattccgg tggtcgccgt
    30901 tgccagccat gataccgcca gcgcggttat cgcctcgccg ttaaacggtt cacgcgctgc
    30961 ttatctctct tctggcacct ggtcattgat gggcttcgaa agccagacgc catttaccaa
    31021 tgacacggcg ctggcagcca acataaccaa tgaaggcggg gcggaaggtc gctatcgggt
    31081 gctgaaaaat attatgggct tatggctgct tcagcgagtg cttcaggagc ggcaaatcaa
    31141 cgatctcccg gcgcttatcg ccgcgacaca ggcacttccg gcctgccgct tcatcatcaa
    31201 tcccaatgac gatcgcttta ttaatcctga ggcgatgtgc agcgaaattc aggctgcgtg
    31261 tcgggaaatg gcgcaaccgc tcccggaaag tgatgctgaa ctggcgcgct gcattttcga
    31321 cagtctggcg ctgctgtatg ccgatgtgtt gcatgagctg gcgcagctac gcggtgaaga
    31381 tttctcgcaa ctgcatattg tcggcggcgg ctgccagaac acgctgctca accagctatg
    31441 tgccgatgcc tgcggtattc gggtgatcgc cgggcctgtt gaagcctcga cgctcggcaa
    31501 tatcggcatc cagttaatga cgctggatga actcaacaat gtggatgatt tccgtcaggt
    31561 cgtcagcacc accgcgaatc tgaccacctt tacccctaat cctgacagtg aaattgccca
    31621 ctatgtggcg cagcttcact cgacacgaca gacaaaggag ctttgcgcat gaccactcaa
    31681 ctggaacagg cctgggagct ggcgaaacag cgtttcgcgg cggtggggat tgatgtcgag
    31741 gaggcgctgc gccaacttga tcgtttaccc gtttcaatgc actgctggca gggcgatgat
    31801 gtttccggtt ttgaaaaccc ggaaggttcg ctgaccggtg ggattcaggc tattggtaat
    31861 tatccgggca aagcgcgtaa tgccagtgag ctacgagccg atctggaaca ggctatgcgg
    31921 ctgattccgg ggccgaaacg gcttaattta catgccatct atctggaatc agacacgcca
    31981 gtctcgcgcg accagatcaa acctgagcac ttcaaaaact gggttgaatg ggcgaaagcc
    32041 aatcagctcg gtctggattt taacccctcc tgtttctcgc atccgctaag cgccgatggc
    32101 tttacgctct cgcatcccga cgacagcatt cgccagttct ggattgatca ctgcaaagcc
    32161 agccgtcgcg tttcggccta ttttggcgag caactcggca caccatcggt gatgaacatc
    32221 tggatcccgg atggtatgaa agatatcacc gttgaccgtc tcgccccgcg tcagcgtctg
    32281 ctggcagcac tggatgaggt gatcagcgag aagctggatc cggcgcacca tatcgacgcc
    32341 gttgagagca aattgtttgg cattggcgca gagagctaca cggttggctc caatgagttt
    32401 tacatggggt atgccaccag ccgccagact gcgctgtgcc tggacgccgg gcatttccac
    32461 ccgactgaag tgatttccga caagatttcc gccgccatgc tgtatgtacc gcagttgctg
    32521 ctgcacgtca gccgtccggt tcgctgggac agcgatcacg tggtgctgct ggatgatgaa
    32581 acccaggcga ttgccagtga gattgttcgt cacgatctgt tcgaccgggt gcatatcggc
    32641 ctcgacttct ttgatgcctc tatcaaccgc attgccgcgt gggtcattgg tacacgcaat
    32701 atgaaaaaag ccctgctgcg tgcgttgctg gaacctaccg ctgagctgcg caagctggaa
    32761 gcggcgggcg attacactgc gcgtctggcg ctactggaag aacagaaatc gttgccgtgg
    32821 caggcggtct gggaaatgta ttgccaacgt cacgatacgc cagcaggtag cgaatggctg
    32881 gagagcgtgc gggcttatga gaaagcgatt ttgagccagc gtgggtaaac actgccggat
    32941 gcggcgcgag cgccttatcc ggcctacagg tcgaatgacc atgcgtgctg ccaccctcac
    33001 cccggccctc tccctgaaag ggcgagggag aaaaacatag cctcgtgcgg caacggagct
    33061 gaacaccgct ccggacagtc ccctcgcccc tacggggaga gggttagggt gaggggaaca
    33121 agccgcacca tgtctaaaca aggcaaccta atttacagga acacagaaca tgcaaaacat
    33181 tacccagtcc tggtttgtcc agggaatgat caaagccacc accgatgcct ggctgaaagg
    33241 ctgggacgag cgcaatggtg gtaacctgac gctgcgcctg gatgacgccg atatcgcgcc
    33301 ttatcacggc aactttcatg cacaaccgcg ctatattccc ctcagccaac ccatgccttt
    33361 actggcaaat acgccgttta ttgtcaccgg ctccggcaaa ttcttccgta acgtccagct
    33421 tgatcctgca gctaacttag gcgtcgtaaa agtcgacagc gacggcgcgg gctaccatat
    33481 tctctggggg ttaaccaacg aagccgttcc aacttccgaa cttccggctc acttcctttc
    33541 ccactgcgag cgcattaaag ccaccaacgg taaagatcgg gtgatcatgc actgccacgc
    33601 caccaacctg atcgccctca cctatgtact tgaaaacgac accgcggtct ttactcgcca
    33661 actgtgggaa ggcagcaccg agtgtctggt ggtattcccg gatggcgttg gcattctgcc
    33721 gtggatggtg cccggcacgg acgaaatcgg ccaggcgacc gcacaagaga tgcaaaaaca
    33781 ttcgctggtg ttgtggccct tccacggcgt cttcggcagc gggtcgacgt tggatgaaac
    33841 attcggttta atcgacaccg cagaaaaatc agcacaagta ttagtgaagg tttattcgat
    33901 gggcggcatg aaacagacca tcagccgtga agagttgata gcgctcggca agcgcttcgg
    33961 cgttacacca ctagctagtg cgctggcgct gtaaggagca aacatgatcc gcaaagcctt
    34021 tgtcatgcag gttaaccccg acgcccacga agagtatcag cgtcggcata accccatctg
    34081 gccggaactg gaagcagtgt tgaaatctca cggtgcgcat aactacgcca tttacctcga
    34141 caaagcgcgt aatctgctgt ttgccacggt agagattgaa tctgaagaac ggtggaatgc
    34201 ggtagccagc actgatgttt gccaacgttg gtggaaatat atgaccgatg ttatgcccgc
    34261 taacccggat aacagcccgg tgagtagcga gctgcaagaa gtgttttacc tgccgtaatt
    34321 gcccaatgtg ccagagatgc ctccggcaca atattttaaa tttcgatttg aaaaattaaa
    34381 catattacat ctcccggtaa gttatatttc cctgatacat tgtgagtaaa tcacaaaaat
    34441 aatgaataac ccattaatga ttcatgtggt ttatttaaat aaccattatg tgcattactc
    34501 cgcaaatctg acctttcact ctgtctcaaa ttgtcattgt ttaccgacat atcgcctctc
    34561 atcacttaat cgctccacca ttacaattga ttaaaatata ttttatggag tccgattaat
    34621 ggcagctctt actgcaagct gtattgacct gaatattcag ggcaatggcg cttattccgt
    34681 tctgaagcag ttggcgacaa tagcgttaca aaacggtttt atcaccgact cacaccagtt
    34741 cctgcaaacc ctgctgctgc gcgaaaaaat gcactctact ggatttggtt ccggtgtcgc
    34801 cgtgccgcac ggtaaaagcg cctgcgttaa acaaccgttt gtattattcg cccgcaaagc
    34861 gcaggctatt gactggaaag ccagcgatgg cgaagacgtc aattgctgga tctgcctcgg
    34921 cgtgccgcaa agcggcgaag aggatcaggt caaaatcatc ggcacactgt gtcgcaaaat
    34981 tatccaccag gattttattc atcaactgca acagggcgac gccgaccagg tgcttgcctt
    35041 gttaaatcaa accctcagct cataaggaag tggcgatgga gtcatcctta tgtattgtcg
    35101 cgatcaccaa ctgccccgcc gggatcgctc acacctacat ggtggcggaa gccctggaac
    35161 agaaagcccg ttctctcggc cataccataa aagtggaaac tcaagggtcc agtggcgttg
    35221 aaaaccgctt atccagcgaa gagattgccg ctgccgatta cgtcattctc gctaccgggc
    35281 gtggcctgag cggtgatgat cgcgcgcggt ttgccgggaa gaaagtttat gagattgcca
    35341 tctcccaggc gttgaaaaat atcgaccaga ttttcagcga attaccgaca aactcgcagc
    35401 tttttgccgc agatagcggc gtgaagcttg gcaaacagga agtgcaaagc ggcagcgtga
    35461 tgagccacct gatggcgggc gtctctgcgg cgctgccgtt tgtcatcggc ggtggtatcc
    35521 tggtggcgct tgccaacatg ctggtgcagt tcggtttgcc gtataccgat atgtcgaaag
    35581 gcgcgccgtc gtttacctgg gtagttgaat ccatcggtta ccttggcttc acctttatga
    35641 tccccatcat gggcgcttat attgcctcgt cgattgccga taaaccggct ttcgctccgg
    35701 cgtttctggt ttgctatctg gcgaacgaca aagcgctgct cggcacccag tcgggtgcgg
    35761 gattcctcgg cgcggtggtg tttctggttt gctatctggc gaacgacaaa gcgctgctcg
    35821 gcacccagtc gggtgcggga ttcctcggcg cggtggtgct ggggctggcg attggctatt
    35881 tcgtcttctg gttccgcaaa gtgcgccttg gcaaagcatt acaaccgctg ctcggttcaa
    35941 tgctgatccc gttcgttact ctgctggttt tcggcgtgct cacttactac gttatcgggc
    36001 cggtgatgtc cgacctcatg ggcgggctgc tccacttcct gaataccatt cctccatcaa
    36061 tgaagtttgc ggcagcgttt ctggtcggtg cgatgctggc gttcgatatg ggcggcccca
    36121 tcaataaaac cgcctggttc ttctgcttct cactgctgga aaaacacatt tatgactggt
    36181 acgccatcgt cggcgttgtc gcgttgatgc cgcccgttgc ggcgggtctt gcgacgttca
    36241 ttgcgcccaa actgtttacc cgacaggaaa aagaggctgc cagtagtgcc attgtggtcg
    36301 gtgccacagt cgcaaccgaa ccggctatac cttacgctct ggcagctccg ttgccgatga
    36361 ttaccgccaa tacgctggcg ggtggcatta ccggcgtgct ggtgatcgct ttcggaatca
    36421 aacgtctggc acctggtttg ggtatctttg acccgctgat cggcctgatg tcgccggtgg
    36481 gttcgtttta tctggtgcta gcgattggcc tcgcgctgaa catttcattc attatcgtcc
    36541 tgaaaggatt gtggttacgc cgcaaagcga aagccgcgca acaggagctt gtccatgaac
    36601 attgagttac tgcaacagtt gtgcgaagcc agcgccgtca gcggcgatga acaggaagtt
    36661 cgcgacattc tgataaacac gctggaacct tgcgttaatg agatcacctt tgatggtctg
    36721 ggcagctttg ttgcccgtaa ggggaataaa ggtccaaaag ttgccgttgt caggcatatg
    36781 gatgaagtcg gctttatggt cacccacatc gacgagagcg gttttctgcg ctttaccacc
    36841 attggcggct ggtggaatca gtcaatgctc aaccaccggg taaccatacg cacacacaag
    36901 ggatttaaaa tccctggtgt gattggttcc gtcgcacctc atgcgttaac ggaaaagcaa
    36961 aagcaacagc cgctgtcatt tgatgagatg ttcattgata ttggcgcgaa cagtcgcgat
    37021 gaggtggaaa aacgcggtgt ggcgatgggc gattttatta gcccggaagc caattttgcc
    37081 tgctggggcg aagataaagt ggtcggcaag gcgctggata accgcatcgg ctgcgcaatg
    37141 atggctgaac tattgcagac ggtgaataat cccgaaatta cgctgtatgg cgtcggcagc
    37201 gtggaagaag aagttgggct acgcggggca caaacctcgg cggaacacat taaaccggat
    37261 gtggtgattg tgctggatac cgctgtcgca ggtgatgttc cgggcattga taacattaaa
    37321 tacccgctga aactgggcca ggggccgggg ctgatgctgt ttgacaagcg ctacttcccc
    37381 aaccagaaac tggtggcggc gttaaaaagc tgtgccgcac ataacgattt accgctgcaa
    37441 ttttccacca tgaaaaccgg tgcgacggat ggcgggcgct acaacgtgat gggcggcggg
    37501 cgtccggttg ttgcgctgtg cctgccaacc cgctatctgc acgctaacag cggcatgatt
    37561 tcaaaagccg attacgatgc tctgctcacg ttgatacggg attttctgac caccttaacc
    37621 gcggagaaag tcaacgcgtt tagccagttc cgtcaggtgg attaatgcta aacgaacgcc
    37681 agttaaagat tgtcgatctg ctggagcaac agccgcgcac gcctggtgag ctggcgcaac
    37741 agactggcgt ttcaggcagg accatcctgc gtgatattga ctatctcaac tttaccctta
    37801 acggcaaagc ccgcattttc gccagtggca gtgcgggcta tcagctggaa atcttcgagc
    37861 gccgcagctt ttttcagttg ctgcaaaagc acgataacga cgatcggctg ctggcgctgt
    37921 tattactgaa tacctttact ccccgtgcgc aactcgcctc ggcgcttaat ttgccagaaa
    37981 cctgggtagc agagcgtctg ccccggttaa aacagcgtta tgaacgcact tgttgcctgg
    38041 ccagtcgccc cggtttgggc catttcattg atgagacaga agagaaacgc gttatcttgc
    38101 tggcgaactt gctgcgcaaa gatccgtttt taattccgct ggcgggcgta acacgagaca
    38161 attttcagca tttatccacg gcctgcgaca accaacaccg ctggccgctc atgcagggtg
    38221 attatctctc cagcctgatt ctggcgattt acgccctgcg taatcaactg accgatgagt
    38281 ggccgcaata tcccggtaac gagataaaac aaatcgttga acagagcggc atgtttcttg
    38341 gtgataacgc tgtaagaacc ctgacaggtt tgatagagaa acagcatcag caagcgcagg
    38401 taatttcagc cgatcatgtg ctggggttgc tgcaaagggt gccaggcatc gcgtcattga
    38461 atattattga tacgcagctg gttgagaata ttaccgggca tttattacgt tgccttgccg
    38521 caccggtgtg gattgccgag caccgccaaa gcagcatgaa taacctgaaa gccgcctggc
    38581 ctgcggcctt tgatatgagt ctgcacttta ttacgctact gcgtgaacag ctcgatattc
    38641 cccttttcga tagcgatctg atcggtttgt attttgcctg tgcgctggaa cggcatcaaa
    38701 acgaacgcca gccgatcatt ttgctctcgg accagaacgc gattgccact attaatcagc
    38761 tcgccattga gcgcgatgtt ttacattgtc gggtgattat tgcccgtagc ttaagcgagc
    38821 ttattgccat tcgcgaagag attgagccgt tattgatcat taacaacagc cattatttac
    38881 tggatgacgc ggtaaataat tacatcaccg taaaaaatat cattacggct gccgatatcg
    38941 aacaaataaa acactttctg gcgacggcat ttattcgcca acagccggaa cgctttttct
    39001 ctgcccccgg aagttttcat tattcgaatg tacgcggtga aagctggcaa catattaccc
    39061 ggcaaatttg tgcgcaatta gtggcacaac accatattac cgccgatgaa gcgcaacgga
    39121 tcatcgcccg cgaaggcgaa ggtgaaaacc tgattgttaa tcgcctcgcc atcccacatt
    39181 gctggagcga acaggagcga cgttttcgtg gattttttat taccctcgcc caaccagttg
    39241 aggtgaataa cgaagtcatt aaccatgtct tgatcgcctg cgccgccgcc gatgcgcgtc
    39301 atgagctaaa aatatttagc tatctggcaa gcgtattgtg tcagcatccg gcagaagtta
    39361 ttgccgggtt aacaggatat gaggcattta tggagttact tcacaagggg tgaactgtgt
    39421 cggatgcgcc gcatccgaca ctgcattttc atcaaaaacg tttaacgcag gctattatag
    39481 tcatcaacca tttcgttgta ctcgcgtaac gctcgtggaa atgagtcttc gaccatccag
    39541 cttgtgtttg catcctgaag atgctcttta tcccagtcag agtacgggac tttatctctg
    39601 acgcggcgaa catatttttt agtctcgagt tgatactggt ctgccgaatt aatgaacgag
    39661 aaattcatac cgcctttatc cgcttctttg gcctgcgcta ccagcgtttc cagttcagag
    39721 actttcttca ttgccgcatc aacatcaa
//
LOCUS       DAETQN010000023        39477 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.023, whole genome shotgun sequence.
ACCESSION   DAETQN010000023 DAETQN010000000
VERSION     DAETQN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39477
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.023"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(234..1574)
                     /gene="fadL"
                     /locus_tag="K0W68_001256"
     CDS             complement(234..1574)
                     /gene="fadL"
                     /locus_tag="K0W68_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBI3072868.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1946..2230
                     /locus_tag="K0W68_001257"
     CDS             1946..2230
                     /locus_tag="K0W68_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBI3072869.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2410..3720
                     /gene="fadI"
                     /locus_tag="K0W68_001258"
     CDS             2410..3720
                     /gene="fadI"
                     /locus_tag="K0W68_001258"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBI3072870.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3720..5864
                     /gene="fadJ"
                     /locus_tag="K0W68_001259"
     CDS             3720..5864
                     /gene="fadJ"
                     /locus_tag="K0W68_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HBI3072871.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6067..6552
                     /gene="sixA"
                     /locus_tag="K0W68_001260"
     CDS             6067..6552
                     /gene="sixA"
                     /locus_tag="K0W68_001260"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBI3072872.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7236..7799
                     /locus_tag="K0W68_001261"
     CDS             7236..7799
                     /locus_tag="K0W68_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI3072873.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7881..10523
                     /gene="yfcU"
                     /locus_tag="K0W68_001262"
     CDS             7881..10523
                     /gene="yfcU"
                     /locus_tag="K0W68_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HBI3072874.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            10543..11294
                     /locus_tag="K0W68_001263"
                     /pseudo
     CDS             10543..11294
                     /locus_tag="K0W68_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            11269..11817
                     /locus_tag="K0W68_001264"
     CDS             11269..11817
                     /locus_tag="K0W68_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3072875.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            11814..12284
                     /locus_tag="K0W68_001265"
     CDS             11814..12284
                     /locus_tag="K0W68_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3072876.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            12281..12805
                     /locus_tag="K0W68_001266"
     CDS             12281..12805
                     /locus_tag="K0W68_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3072877.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            12792..13664
                     /locus_tag="K0W68_001267"
     CDS             12792..13664
                     /locus_tag="K0W68_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBI3072878.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(13786..14337)
                     /gene="smrB"
                     /locus_tag="K0W68_001268"
     CDS             complement(13786..14337)
                     /gene="smrB"
                     /locus_tag="K0W68_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBI3072879.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            14503..15435
                     /gene="prmB"
                     /locus_tag="K0W68_001269"
     CDS             14503..15435
                     /gene="prmB"
                     /locus_tag="K0W68_001269"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBI3072880.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            15470..16555
                     /gene="aroC"
                     /locus_tag="K0W68_001270"
     CDS             15470..16555
                     /gene="aroC"
                     /locus_tag="K0W68_001270"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBI3072881.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            16559..17383
                     /gene="mepA"
                     /locus_tag="K0W68_001271"
     CDS             16559..17383
                     /gene="mepA"
                     /locus_tag="K0W68_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBI3072882.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            17383..18192
                     /locus_tag="K0W68_001272"
     CDS             17383..18192
                     /locus_tag="K0W68_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBI3072883.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            18192..18740
                     /gene="epmC"
                     /locus_tag="K0W68_001273"
     CDS             18192..18740
                     /gene="epmC"
                     /locus_tag="K0W68_001273"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBI3072884.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            18774..19052
                     /locus_tag="K0W68_001274"
     CDS             18774..19052
                     /locus_tag="K0W68_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBI3072885.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(19173..21179)
                     /gene="mnmC"
                     /locus_tag="K0W68_001275"
     CDS             complement(19173..21179)
                     /gene="mnmC"
                     /locus_tag="K0W68_001275"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBI3072886.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            21338..22558
                     /gene="fabB"
                     /locus_tag="K0W68_001276"
     CDS             21338..22558
                     /gene="fabB"
                     /locus_tag="K0W68_001276"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBI3072887.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            22833..24011
                     /locus_tag="K0W68_001277"
     CDS             22833..24011
                     /locus_tag="K0W68_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3072888.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(24008..25003)
                     /gene="flk"
                     /locus_tag="K0W68_001278"
     CDS             complement(24008..25003)
                     /gene="flk"
                     /locus_tag="K0W68_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBI3072889.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            25102..26238
                     /gene="pdxB"
                     /locus_tag="K0W68_001279"
     CDS             25102..26238
                     /gene="pdxB"
                     /locus_tag="K0W68_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBI3072890.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            26304..27317
                     /locus_tag="K0W68_001280"
     CDS             26304..27317
                     /locus_tag="K0W68_001280"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBI3072891.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            27317..28129
                     /gene="truA"
                     /locus_tag="K0W68_001281"
     CDS             27317..28129
                     /gene="truA"
                     /locus_tag="K0W68_001281"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBI3072892.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            28212..28871
                     /locus_tag="K0W68_001282"
     CDS             28212..28871
                     /locus_tag="K0W68_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3072893.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            29027..29941
                     /gene="accD"
                     /locus_tag="K0W68_001283"
     CDS             29027..29941
                     /gene="accD"
                     /locus_tag="K0W68_001283"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBI3072894.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            30011..31279
                     /gene="folC"
                     /locus_tag="K0W68_001284"
     CDS             30011..31279
                     /gene="folC"
                     /locus_tag="K0W68_001284"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBI3072895.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            31269..31931
                     /gene="dedD"
                     /locus_tag="K0W68_001285"
     CDS             31269..31931
                     /gene="dedD"
                     /locus_tag="K0W68_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBI3072896.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            32190..32678
                     /gene="cvpA"
                     /locus_tag="K0W68_001286"
     CDS             32190..32678
                     /gene="cvpA"
                     /locus_tag="K0W68_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBI3072897.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            32715..34232
                     /gene="purF"
                     /locus_tag="K0W68_001287"
     CDS             32715..34232
                     /gene="purF"
                     /locus_tag="K0W68_001287"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBI3072898.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            34327..34896
                     /gene="ubiX"
                     /locus_tag="K0W68_001288"
     CDS             34327..34896
                     /gene="ubiX"
                     /locus_tag="K0W68_001288"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBI3072899.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            35162..35944
                     /gene="argT"
                     /locus_tag="K0W68_001289"
     CDS             35162..35944
                     /gene="argT"
                     /locus_tag="K0W68_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBI3072900.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            36165..36947
                     /gene="hisJ"
                     /locus_tag="K0W68_001290"
     CDS             36165..36947
                     /gene="hisJ"
                     /locus_tag="K0W68_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBI3072901.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            37037..37723
                     /gene="hisQ"
                     /locus_tag="K0W68_001291"
     CDS             37037..37723
                     /gene="hisQ"
                     /locus_tag="K0W68_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBI3072902.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            37720..38436
                     /locus_tag="K0W68_001292"
     CDS             37720..38436
                     /locus_tag="K0W68_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3072903.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            38444..39217
                     /gene="hisP"
                     /locus_tag="K0W68_001293"
     CDS             38444..39217
                     /gene="hisP"
                     /locus_tag="K0W68_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBI3072904.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
BASE COUNT         9349 a         9671 c        10613 g         9844 t
ORIGIN      
        1 gtatcctgga aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat
       61 atgcctttcc cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagctgc
      121 caacaggctg tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg
      181 ggggagttaa agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac
      241 gcgtagttaa agttagtacc gaacagccag gctttacctt cagactcgaa ctggtatggg
      301 ccttcgttaa ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca
      361 tctttattaa atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg
      421 gagatagaac gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag
      481 gtccagttat catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag
      541 ccttcatgtt tctggaacag cgtgtcgcca ctggttgagg tcgctttcag ctgctggaac
      601 tgactccagc tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta
      661 taacctgaca cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg
      721 ccacctgtcg cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg
      781 ctgtagttac ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta
      841 tttttatcca gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc
      901 agatgagcga ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct
      961 tgaggagttt gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg
     1021 cctgcgaaac gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc
     1081 catgcattat ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt
     1141 gtacccccga cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta
     1201 gaggtaatag aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc
     1261 ggaacccatg ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt
     1321 ccgctgatat tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca
     1381 aacatggtaa tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct
     1441 tcccctgaat aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc
     1501 gaccaggcct gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg
     1561 gttttctggc tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc
     1621 tgtaacagga gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga
     1681 gagtataggt cggaccagct ggaatgctgc aagtatgttt ccatcttttt tcaaatatga
     1741 tctaagaaac gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga
     1801 attttgtttt agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta
     1861 acaactggag cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg
     1921 ggtgcacata cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg
     1981 ctgctgtatg gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg
     2041 cgtgtttgca aaccgcgcag aagcagaaca aacgctggcg gcgctgactg aaaaagcccg
     2101 tagcgtggaa tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt
     2161 gcgcctggat atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg
     2221 tctgcgttaa ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct
     2281 tttctttctc ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta
     2341 aatgtaaaaa aatagttaag actatgatca ggtcagacca ctttatttat ttttttacag
     2401 gggagtgtta tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt
     2461 gttagcggtt tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg
     2521 gttgatttag ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa
     2581 gtgattgaac agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg
     2641 cgtgaaattg ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc
     2701 gcttgcgcta ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact
     2761 atccgagcgg ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt
     2821 aaaaaactgg cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg
     2881 aaactcttct ctcgcctgcg tttgtgcgac ttaatgcccg taccgcctgc ggtagcagaa
     2941 tattctactg gcttgcggat gggcgacacc gccgaacaaa tggcgaaaac ctacggcatc
     3001 acccgagaac agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg
     3061 tcagacggaa aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg
     3121 cttgtcgaag acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc
     3181 ccggcgtttg atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat
     3241 ggcgcggcag cggtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg
     3301 ctggggtatc tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgctc
     3361 ggtccagcct ggtcaacacc gctggcgctg gaacgtgccg gtttgacgat gagcgaactg
     3421 acattgatcg atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg
     3481 ggtagtgaac gttttgctcg tgaagtactg gggcgtgcac atgccactgg cgaagtggac
     3541 gatagcaaat ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc
     3601 ggcgcgcgga tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt
     3661 ttagttaccg cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa
     3721 tggaaatggc atcagcgttt actcttaatg ttcgtctgga caacattgct atcatcacca
     3781 tcgatgtacc ggatgagaaa atgaataccc tgaaggcgga atttgcctcg caggtgcgcg
     3841 ccattattaa gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga
     3901 aaccggacaa ttttatcgcc ggagcagaca tcaacatgat tggcaactgc aaaacggcgc
     3961 aagaagcgga agcactggcg cggcagggcc aacagttgat ggcggagatt catgctttgc
     4021 ccattccggt tatcgcggct attcatggcg cttgcctggg cggtgggctg gagctggcgc
     4081 tggcgtgcca cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag
     4141 tacaacttgg attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg
     4201 tcagtacagc attagagatg attctcaccg gaaaacaact tcgggcgaaa caggcattaa
     4261 agctggggct ggtggatgac gttgttccgc actccatttt gctggaagcc gctgttgagc
     4321 tggcgaagaa ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg
     4381 ggccgttagg tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc
     4441 aaggcaatta tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg
     4501 gcaccagcag cggctatgac gctgaagctc gcgcctttgg cgaactggcg atgacgccgc
     4561 aatcgcaagc tttgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta
     4621 gtgatgcacc gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg
     4681 gcggtattgc ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca
     4741 acccgcaggg cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc
     4801 gccgtcgtca tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga
     4861 cggactatcg cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg
     4921 aattaaaaca acagatggtg gcggaagtgg agcaaaattg cgccgctcat accatttttg
     4981 cttcgaacac gtcatcttta ccgattggtg atatcgccgc tcacgccgcg cgacctgagc
     5041 aagttattgg cctgcatttc ttcagtcctg tggaaaaaat gccgctggta gagattattc
     5101 ctcatgcggg tacatcggcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg
     5161 gcaaaacgcc cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc
     5221 cttacattaa tgaagctatc cgcatgctga ccgaaggtga acgggtagag cacattgatg
     5281 ccgcgctagt gaaatttggt tttccggtag gcccaatcca acttttggat gaggtaggaa
     5341 tcgacaccgg gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg
     5401 cgcctgcaaa tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc
     5461 ggggtttcta tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca
     5521 tttacccgct cattggcgca caagggcagg ggcgactctc cgcaccgcag gttgctgaac
     5581 ggtgtgtgat gttgatgctg aatgaagcag tacgttgtgt tgatgagcag gttatccgta
     5641 gcgtgcgtga cggggatatt ggcgcggtat ttggcattgg ttttccgcca tttctcggtg
     5701 gaccgttccg ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac
     5761 tggccacgca gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc
     5821 gtggggaaag tttttggaaa acaactgcaa ctgaccttca ataagaaggt caaagctata
     5881 tgaatccgcg ctgaatggcg gagtgttggt caaaatgtaa acgcatattg actatactta
     5941 cgccattgag gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca
     6001 ccgttttctt tctctggttt cagatgaaag aaaacggacg aatctggtta acaaaagcgg
     6061 tgcaatatgc aagtttttat catgcgtcac ggcgacgcag cccttgatgc cgccagtgat
     6121 tcggttcgtc ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg
     6181 aaaggtcaaa aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa
     6241 acactggaag aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag
     6301 ttaacgccct gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa
     6361 ggtgttgctt cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag
     6421 ttatgtccgg gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt
     6481 gatgaaagcg gaaacggcac attcaactgg cagatgagcc cgtgtaatct gaagatggca
     6541 aaagctatct gatgagttcc aaattatgcc caatgaggta gattcattgg gcataattgc
     6601 tcatcaggca gcggatatct gtcaatctca ctactatcga agactctccc catctataaa
     6661 taaaacactt tctactttca ttcggaaatt attcttctgc gcgtatcttg attttgccca
     6721 aagttacatg ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga
     6781 taatagagaa aatagagttg aataatcagg caggatgtaa ttgaaacttg attaaattct
     6841 ggctatagtg tttatatatt atattgtcaa aagtggataa atgccaatcg cattatatgt
     6901 tcattgtttg ttttttaagc tgttgatatt cattattgat atactttcta ttttttctaa
     6961 atacttattc cgtatttttt aaggatgtcc ttaagataac ctttcttctg tcaatatatt
     7021 aattaaatct acatttactc tttagtaaaa ttaaattgca tcaggggtgt ggaaacatca
     7081 ctatgcggcc ccacaccaac gaatacaatt ttctacgacc tgttcttttt ggatcgcatg
     7141 ccgtattcaa tggctgggta taaacattgg ttattgactg aatcaatgta ttgcaatgtt
     7201 tatattttta attatttata gtttaaggac aaataatgag taagtttgtt aaaacagcta
     7261 ttgctgcggc aatggtgatg ggcgtgttca cctctacggc aacaatcgct gcgggtaaca
     7321 acggtactgc acgtttttac ggcaccattg aagactcagt gtgttctatc gttccggacg
     7381 atcacaaact ggaagttgat atgggtgata tcggtgccga aaaactgaaa aataacggca
     7441 ccaccacgcc gaaaagtttc cagattcgtc tgcaagattg cgtatttgat actcaggaaa
     7501 caatgaccac tacctttact ggtaccgttt cttctgcaaa tagcggcaat tattacacca
     7561 ttttcaatac cgatacctgt gcggcattta acaatgttag cctggcgatc ggtgactctc
     7621 tgggtacctc ttacaaaagc ggcatgggta ttgaccagaa aatagtgaaa gacacttcta
     7681 ccaataaagg caaagcgaag cagacactga actttaaagc ctggctggtc ggcgcagctg
     7741 atgcgccaga tctgggtaat ttcgaagcaa ataccacctt ccagattact tacctgtaag
     7801 gcataccccc atagcgacac tgctatgggg gatttaaatg gattttgacc gaacaagaat
     7861 aaaaacggat agaaacgtgt atgcctgacc attctctttt tcgattacgg attcttccgt
     7921 ggtgcattgc gctggcaatg tcagggagtt atagcagtgt ctgggctgaa gacgacattc
     7981 agtttgattc ccgttttctg gaattaaaag gcgacacaaa aattgatctg aagcgttttt
     8041 ccagccaggg atatgttgag cccggaaaat acaatttaca ggttcaacta aataaacagc
     8101 cattggcgga agagtacgat atttactggt atgctggtga agatgacgcg agcaaaagct
     8161 atgcttgtct gacaccggaa ctggtagcgc agtttggttt aaaagaagac gtggcgaaaa
     8221 atctgcaatg gagccacgat gctaaatgcc tgaaatccgg tcaactggaa ggcatggaaa
     8281 ttaaggctga tttaagccag tccgcattag tcatttcact gccacaggct tacctcgaat
     8341 atacttatcc cgactgggat ccgccttcac gttgggatga cggcatctcc gggatcgtcg
     8401 cggactacag catcaacgca caaacccggc acgaagaaaa tggcggtgat gatagtaacg
     8461 agatcagcgg caacgggacg gtcggggtta acctggggcc gtggcgtatg cgtgctgact
     8521 ggcagactaa ctatcaacat actcgcagta atgatgacga tgaattcagc ggcgatgaaa
     8581 ctcaaaaaaa atgggagtgg agtcgctact atgcctggcg ggcgttacca tcattaaaag
     8641 ccaaactggc gctgggcgag gattacctca gatccgatat ttttgatggt tttaactatg
     8701 ttggtggcag tgtcagtact gacgatcaaa tgttgcctcc caatctgcgc ggctacgcgc
     8761 cagacatttc cggcgtggca cacaccacag caaaagtgac cgtcagccag atggggcgtg
     8821 tgatttacga aacgcaggtt ccggctggac cgtttcgtat tcaggatctt ggtgattctg
     8881 tctccggtac gttgcatatt cgcattgaag aacagaacgg ccaggtgcag gaatatgaca
     8941 tcagcaccgc ctcgatgcca tacctcactc gtccaggtca ggttcgctat aagatcatga
     9001 tgggccgtcc gcaagagtgg ggacaccatg tcgagggtga atttttttct ggtgctgaag
     9061 cttcctgggg gatcgctaac ggctggtcgt tatatggcgg cgcactggga gatgaaaact
     9121 atcagtctgc ggcgcttggc gtcggtcgcg atttgtctac attcggcgcg gtcgcgtttg
     9181 atgttactca ctcgcacacc aaactggata aagacaccgc ttatggcaaa ggttcgctgg
     9241 acggtaactc cttccgtgtg agttattcca aagactttga ccagctcaac agccgcgtta
     9301 cttttgctgg atatcgcttc tcggaagaga actttatgac catgagcgag tatctggatg
     9361 ccagtgacag cggaatggta cgcacgggca acgacaaaga gatgtacact gccacttata
     9421 accagaactt ccgcgatgcg ggtgtttcgg tttatctcaa ctatacccgc catacctact
     9481 gggatcgcga ggagcagaca aactacaaca tcatgctctc gcactatttc aatataggca
     9541 gtattcgtaa tgtcagcatc tcgatgactg gctaccgtta cgagtatgac aaccaggccg
     9601 acaaaggcat gtacatttcg ctcagtatgc cgtggggtga caacagtacc gttagctata
     9661 acggtaacta tggcagtggg acggacagca gtcaggtcgg ttatttcagc cgtgtcgatg
     9721 acgcgactca ctatcagttg aacgtcggca ccagtgacaa acacaccagc gttgacggct
     9781 attacagcca tgatggttcg ctggcgcagg ttgacctcag tgcgaactac catgaagggc
     9841 aatacacctc tgcgggcttg tcgttacagg gcggcgcaac gcttactgcc cacggtggcg
     9901 cacttcaccg tacccagaat atgggcggga cacgcttgtt gattgatgcc gatggcgttg
     9961 ccgatgttcc ggtagaaggt aacggtgctg ctgtttatac caatatgttt ggtaaagccg
    10021 tcgtttctga cgtcaataac tattaccgca atcaggcgta tatcgacctc aacaaactgc
    10081 cggaaaacgc cgaagcaacc cagtcggtgg tacaagccac gctaactgaa ggggcgattg
    10141 gctaccgcaa atttgccgtc atcagtggtc aaaaagcgat ggcggtgctg cgtttacaag
    10201 acggcagcca tcctccgttt ggcgcagaag taaaaaatga taacgagcag acagtgggcc
    10261 ttgtcgatga tgacggcaat gtttatctgg ctggggtgaa acctggcgaa cacatgagtg
    10321 tgttctggag tggtgttgcg cattgcgata tcaacctgcc ggacccgctg cctgccgatc
    10381 tgtttaacgg cttgttactg ccatgccagc ataaaggcaa cgtcgcgccg gttgttcctg
    10441 atgacataaa gcctgtcatt caggagcaga cgcaacaggt gacacccacc aatccccctg
    10501 tttctgtttc agctaaccaa taacgtgatt aaggaatgat ccatgtctga tttactttgc
    10561 tctgccaaat tgggtgctac gacgctagcg ttactgctta gcgctgccag tttgtcgccc
    10621 aggcttctgt aacgccagat cgtacccgtt tgatatttaa tgaaagcgac aaatcaatca
    10681 gcgtgacgtt gcgcaataac gatccgaaac tgccttatct ggcccaaagc tggatcgaag
    10741 atgaaaaggg taacaagatc agttcaccgt taactgttct gccaccggtg caacgcatcg
    10801 attcaatgat gaacgggcag gtaaaagtgc agggaatgcc ggatatcaac aaattgcctg
    10861 ccgatcgtga aagcctgttc tacttcaacg tgcgtgagat cccgccaaaa tcaaacaaag
    10921 ccaacacgct gcaaatagca ctacaaacgc gtattaagtt gttctggcga ccgaaggcgc
    10981 tggagaacgt cagcatgaaa aacccctggc aatataaggt tactttaacc cgcaacggtc
    11041 aggaatttac cgtcaataac ccgacgccgt attacgtcat tttcagtaat gccagcacac
    11101 agaaaaatgg caatcctgca gccggtttct cgccaatagt gatgtcgccg aaaacctctg
    11161 caccgctgaa tgtgaagatg ggtagcgtgc cggtactgac ctatgtcaac gactatggcg
    11221 ctcggatgcc gctctttttc acttgcaacg gtaacagttg ccaggtcgat gaagagcaga
    11281 gccgcaaagg ctgaggccgg gagagccagt atgaaaggat cttgttctgt ctgggcggta
    11341 actgggttac tgatatgttg tgttggtagc ggctttgcag ttgcagcgag taagtcagta
    11401 agtatgaatt tacgggtgct tgtcgatgcc cctcctcctt gtacggtaaa tggtgcggca
    11461 gttgagtttg gtaatgtatt cattaataag atcaatgggg ttgattataa acgtcccatt
    11521 gactactcgc ttgtttgtaa caatctggca atggatgacc tgcgtctaca gatgcaggcg
    11581 acaacggtcg tgattaacgg tgaaacagtt atcagcacgg gtattcctgg gtttggtatt
    11641 cgggttcaga aaagtagtga tcacacaata ctcgacttaa cctctggctc atggttgcca
    11701 tttaattttt cctcgggagt tcccgttctg gaagctgtgc cggtgaaaca gagtggtaca
    11761 acgttagcgg cagctgaatt taacgccagt gccacaattg tggtggatta ccaatgaaac
    11821 ggatctcgct tattttgctg tggggctttt gctcgatggc actctcaaac gtctcttttc
    11881 acggttattt agtgcagccg cctaactgca cgattagcaa cgctcaaacc attgaaataa
    11941 cttttcagga tgtgcttatt gatgacatta atggcagcaa ttatgagcaa accgtgccgt
    12001 attccattac ctgcgatacg gcggttcgcg acccattaat ggaaatgacc ttgagctgga
    12061 gtggcacact aagcgatttt gataatgctg cagtaagcag caatatcact ggtttaggca
    12121 ttcagctcaa acaggcaggg cagagtttta cgatcaatac gccacttatg gtgaatgaaa
    12181 ccgatttacc ggtgttaacg gctgtgccag taaaaaagag tggtgtcgtt ctgccagagg
    12241 cggactttga ggcctgggcc acgttacagg tagattacca atgaagaaaa agcgtacgtt
    12301 gttttttatt agtagcctga tgttgctggg cagtggaacc acaatagcag gagataattt
    12361 gcactttacc ggcaatttaa tcagtaaatc gtgtacgcca gtgattaatg gtagccagtt
    12421 ggcagaagta cattttcctg ccattgccgc cagcgattta atgaaccttg ggcagtcaga
    12481 gcgagtgcca ttggtgtttc aattgaaaga ttgtcatagt tctacattgt ttaatgtcaa
    12541 agtaacgttg acaggaacag aagatagtgc tctacccggc tttctggcct ttgactcctc
    12601 gtcatcagca tccggcgctg ggattggtat tgaaactgcg gcaggcacat cggtgccaat
    12661 caacaataca acaggcgtca cgctcccgct gaatcaggga aataacagcc taaattttaa
    12721 tacctggtta caggcaaaga gcgggcgaga tgtaacgtcc ggagacttta gtgccacagt
    12781 gacggcaacc tttgagtatt tctgaaatga aaaaatggac cataattttc gcgagcctaa
    12841 tgctgctcgt tctgagtgtt gtcggcgcat ctaagagtta tgctggagat aaacttatga
    12901 gtgcgagttt tgacagtgtg agagtttatt atgccatgga caaggtaacc ggtgctgtag
    12961 cgagcagtgt tttcaatgtt acggtgataa cgccaaagga ggttacatat gggaaatatg
    13021 attcttttgc gtataaaggc gatacgttgc gggttatttc ctggtctggg tcgggttcag
    13081 ctccaacttt ggtactgact gactttgata caattaataa ctcaaattgc cctggcatag
    13141 atacgaaaat ttttcgttgt gcatatatga cgtttaaaat taccgttgca tctgatgatt
    13201 atggttgccc ttggatcgca tccttttatt cctatacaga tttacccgga tttggaagct
    13261 atacggcccc tacagtgcat aacacaatat gcccaacgat accggtcact tcatacgata
    13321 tctcctggag tgaaaattat gtcagccata ataaggcgtt acgaattcag tccacggggt
    13381 caacagtgac caccacgctt tcgacctacc tgatggaagg cggcagactc tgtgatggca
    13441 gtaatttttc tgataatgat ggcagaggag catactgccg cgcggtatca gaattgttaa
    13501 cctttacctc ttacggttgt gataaatcaa cggtaacggt tacgccaact cgtcatccgg
    13561 taacggataa agtactacac gatattgtgg tgaatgtgaa taccagtagt gggcaaccga
    13621 ttgattcgac gtgtcgtttt cagtatgtat tgaatgaact gtagttcgta gtattgctct
    13681 gatgcgtctt atcaggccta cgaagtgaat tgcaatttat tgaattatca catatttgta
    13741 ggccggataa ggcgtttacg ctgctccggc atttgctgcc agaattcagg gcaactccgg
    13801 cggcaaccac tcttccactt caatcaacac caacaaagcc gcatcaccgc cgtactcttt
    13861 cggtgcctgg tgaaatgcca tcacatgcgg atgttgtgcc agccacagcg gtgtttgttg
    13921 cttcaaaata tgcttcccgt gcccatgcat cacgcaggca caaaacacat gttcgcggcg
    13981 acaggcggca atcaacgcgc ccagttcctg cttggcctgc agctgcgtca gaccgtgtaa
    14041 atccaaaaac aattccggtg aataatcgcc gcggcgcagt ttcttcgcct caaaatggct
    14101 gacatccggg cgaacatatt tcaccggacc ttcggtattt aataacggct gaaactcatc
    14161 ggagaaataa tggctggcat cagcctgctc cgggatcaag cgtttcaccg gcacttcgct
    14221 gattttttta cgctgcggtc ggtggacaat cgtgtcctgc ttaatcttgc gagtccccgc
    14281 catcaactgg cgaaacagag cctggtcctc ctcgctaagt gttgttttct ttttcattta
    14341 tctgtctcta tttctaattt tcctacagtt tacccgactc gtagcacaat cgatcgtttt
    14401 aaacgtattt ttcctcactc accgcgcgtg aatgctgatt tatcgtcatc ttcatggcaa
    14461 actagccgcc gaaattattg cgatcatgcc ctggaggaat acgtggataa aattttcgtt
    14521 gatgaagcag taaatgagct gcaaaccatt caggacatgt tgcgctggtc ggtaagccgc
    14581 ttcagcgcgg caaatatctg gtacggtcac ggcaccgata acccgtggga tgaagccgta
    14641 cagctggtgt taccatcgct ctacctgccg ctggatatcc cggaagatat gcgcaccgcg
    14701 cgtctgacct ccagcgaaaa acaccgtatt gttgaacgcg tgatccgccg cgtcaacgag
    14761 cgcattccgg tggcttacct gaccaacaaa gcctggttct gcggtcatga attttacgtc
    14821 gatgaacgcg tgctggtgcc gcgctcgccg attggtgaac tgatcaacaa taaatttgcc
    14881 ggactcatta gcaagcaacc gcagcatatt ttagatatgt gtaccggtag cggctgcatc
    14941 gccattgcct gtgcttatgc cttcccggaa gcggaagtcg acgcggtaga tatctctccc
    15001 gacgcgctgg cggttgccga acagaacatc gaagaacacg gtctgatcca caacgtcatt
    15061 ccgattcgtt ccgatctgtt ccgtgatttg ccgaaagtgc agtacgatct gattgtcact
    15121 aacccgccgt atgtcgacgc ggaagatatg tccgacctgc caaacgaata ccgtcacgag
    15181 ccggaactgg gcctggcatc tggcaccgac ggccttaaac tgacgcgtcg cattcttgga
    15241 aacgcggcag attaccttgc tgatgatggc gtgttgattt gtgaagtcgg caacagcatg
    15301 gtacatctta tggaacaata tccggatgtt ccgttcacct ggctggagtt tgataacggc
    15361 ggcgatggtg tgtttatgct caccaaagag cagctgctgg cggcccgtga atatttcgcg
    15421 atttataaag attaagtaaa cacgcaaaca caacaataac ggagccgtga tggctggaaa
    15481 cacaattgga caactctttc gcgtaaccac cttcggcgaa tcgcatgggc tggcgctcgg
    15541 ctgcatcgtc gatggtgttc cgccaggcat tccgctgacg gaagcggacc tgcaacatga
    15601 cctcgaccgt cgtcgccctg ggacatcgcg ctataccacc cagcgccgcg agccggatca
    15661 ggtcaaaatt ctctccggtg tttttgaagg cgttactact ggcaccagca ttggcttgtt
    15721 gatcgaaaac actgaccagc gctctcagga ttacagcgca attaaagacg ttttccgccc
    15781 aggccatgct gattacacct acgaacaaaa atacggtctg cgcgattatc gcggcggtgg
    15841 acgttcttcc gcccgcgaaa ccgccatgcg cgtggcggca ggggcgattg ccaaaaaata
    15901 tctcgccgag aaatttggta ttgaaatccg cggctgcttg acccagatgg gcgacattcc
    15961 gctggaaatc aaagactggt cgctggttga acaaaatccg ttcttctgcc cggacccgga
    16021 caaaatcgac gcgttagatg aactgatgcg cgcgctgaaa aaagagggcg actccatcgg
    16081 cgcgaaagtc accgttgttg ccagtggtgt ccccgccgga cttggcgagc cggtctttga
    16141 ccgcctggat gccgacatcg cccatgcgct gatgagcatc aacgcggtga aaggcgtgga
    16201 aattggcgac ggttttgacg tagtggcgct gcgcggcagc cagaaccgcg acgaaatcac
    16261 caaagacggt ttccagagca accatgcggg cggcattctc ggcggtatca gcagcgggca
    16321 gcaaatcatt gctcatatgg cgctgaaacc gacctccagc attaccgtgc cggggcgcac
    16381 tattaaccgc tttggcgaag aagttgagat gatcaccaaa ggccgtcacg atccctgtgt
    16441 cgggatccgt gcagtgccga tcgctgaagc gatgctggcg atcgttttaa tggatcacct
    16501 gttacggcag cgggcgcaaa atgccgatgt gaagactgat attccacgct ggtaaaaaat
    16561 gaacaaaacc gcgattgcgc tgctggctct gcttgccagt agcgctagcc tggcagcgac
    16621 gccgtggcaa aaaataaccc aacctgtgcc gggtagcgca caatcgatag gcagtttttc
    16681 taatggctgt attgtcggcg ctgacacgtt gccgatacag tccgaacatt atcaggtcat
    16741 gcgtaccgat cagcgtcgct atttcggtca cccggatctg gtgatgttta tccagcgtct
    16801 gagtagccag gtgagcaatc tgggcatggg tacggtgctg attggcgata tggggatgcc
    16861 cgctggtggg cgtttcaacg gcggtcatgc cagccaccag accggactgg atgtcgatat
    16921 ctttctgcaa ctgccgaaaa ctcgctggac ctccgcgcag ctcttgcgcc cgcaagcact
    16981 ggacttagtt tcccgcgacg gtaaacacgt tgtctccacg ctgtggaagc cagaaatttt
    17041 cagcttgatc aaactcgctg cccaggacaa agacgtcacg cgcatttttg ttaatccggc
    17101 gattaaacaa caactttgcc ttgatgcggg caccgatcgc gactggttgc gcaaagtgcg
    17161 accctggttc cagcatcgcg cgcatatgca tgtacgatta cgttgccctg ccgatagtct
    17221 ggagtgtgaa gatcaacctt taccgccatc aggcgatggt tgcggggcag aactgcaaag
    17281 ctggtttgca cctctaaaac cgggaacaac aaagcctgag aagaagacac cgcctccgtt
    17341 gccgccttcc tgccaggcgc tactggatga gcacgtgatc taatggaaac gtttaatagc
    17401 ctgtttatgg tttccccgct gttgctggga gttctctttt ttgtcgccat gctggcggga
    17461 tttatcgact cgattgccgg tggcggtggg ttactcacca ttccggcatt gatggctgcg
    17521 ggaatgtctc ccgctaacgc gctggcaacc aataaactgc aagcctgcgg cggctctatt
    17581 tccgccacta tctactttat tcgccgcaaa gtggttagtt taagcgatca gaaactcaat
    17641 attgccatga cctttgtcgg ctcaatgagc ggcgcactgt tggtacagta cgttcaggct
    17701 gatgtcttac ggcagatttt gcccattctg gtgatttgta tcggcctcta tttcttgctg
    17761 atgccaaagc tgggtgaaga agatcgccag cgccggatgt acggtttacc cttcgcgtta
    17821 attgccggtg gctgcgtcgg tttttatgac ggattctttg gcccggctgc cggatcgttt
    17881 tacgcgctgg ccttcgttac gctgtgcgga ttcaacctcg ccaaagccac ggctcacgcc
    17941 aaattactca acgcaacgtc aaacattggc ggtctgctgc tgtttattct cggcggcaaa
    18001 gtgatttggg cgacgggctt tgtcatgctg gtggggcagt tcctcggcgc gcgcatgggt
    18061 tcacggctgg tgttgagcaa aggacaaaag ctgatccgcc cgatgattgt tatcgtctcg
    18121 gcggtgatga gtgccaaatt actttacgat agccacggac aggagatcct ccactggttg
    18181 gggatgaact aatgaacagt acacaccact atgagcagtt gattgaaatt tttaatagct
    18241 gctttgccga tgaatttaat acccgtctga ttaaaggcga cgacgaaccg atctatcttc
    18301 ctgctgatgc ggaagtaccg tataaccgaa ttgtctttgc ccacggcttt tatgccagcg
    18361 caattcatga gatttcgcac tggtgtattg ccggaaaagc gcgccgtgag ctggttgatt
    18421 tcggctactg gtattgcccg gatgggcgtg atgcccaaac gcaaagccag tttgaagatg
    18481 ttgaagtgaa gccgcaggca ctcgactggt tgttctgtgt ggcggcggga tatccgttta
    18541 acgtcagttg cgacaatctg gaaggcgact tcgaaccgga tcgtgttgtg tttcaacggc
    18601 gggtacatgc gcaggtgatg gattatctga caaatggcat tcccgagcgt ccggcacgct
    18661 tcattaaagc actacaaaat tattatcaca cgccggaact tacggcggaa cagttcccgt
    18721 ggccggaagc gctcaactga agccggtgcc acgttttaca atgaggaaag aagatgatcg
    18781 cggagtttga atcacgcatt ctggcattaa tcgacggcat ggttgaccat gccagtgatg
    18841 atgagttgtt tgccagtggg tatttgcgtg gccacctgac attagccatc gcagaactgg
    18901 aaagtggtga tgaccactcc gctcaggcgg tgcatacgac cgttagccag agtctggaaa
    18961 aagccattgg tgccggtgaa ttgtcgccgc gtgaccaggc gctggtgacc gatatgtggg
    19021 aaaacctgtt tcagcaggcg tcacagcagt aagttgcggc gtctggagca ccatgcctga
    19081 tgcgacgctt atcgcgtctt atcaggccta cacgtcacac gtaggtcgga taaggcgttc
    19141 acgccgcatc cggcaaacac tgcgagagca gattaccccg ccttaaccgc tttacccttc
    19201 aacaatttcc gcacccataa ccgattcggg tttaacgccg tcagcgtact ggcatccatc
    19261 ggaatcggtt cgtcgctcat ctgcgccgcc agaatctcgg cacacagcgg ggcagaacac
    19321 aaaccgcgag aacctaaagc cgcaaacata aagagatcgt caaaaaccgg cgcgcttacc
    19381 gcctcatctt tctgctccgc caacgacgca tattccacga gtgttgcctc ataatcggga
    19441 acattgccta ccattggcag atgatcgcgg gtggcacaac gcacaccgca gcgcgcctct
    19501 ttatcactga catcaacctc ttttgcccac tgtgcctgcg ggaaacaatc aatcaaccgc
    19561 tggcgattct gctgctgatc gtcctcactg tacgccgtat cttcgctgcc gcgatgataa
    19621 ctggcaccaa tacaatgatg ttgattcgcc ggattttgtg gcgtgagata accgtcatag
    19681 cacagcacct gcttcagctc tgccagttcc ggcgttgtcg gaatatggct gacctgcccg
    19741 gcaaccgaat acaccgggag agtcgacgtt tggctgaaac ggctgatttg atgcccgttc
    19801 gccagtacca ctacgctgtg tgttgcttgc tgatctcctg caaaattcaa caaccaacag
    19861 tcatccttac gggataaatt ctgtaactga tattgataat aaatctgcaa accctgctgt
    19921 tgcgccagtt ccagcacatt acgggtcagt tctgctgggc acagccaacc accttgcgga
    19981 taagtaatgc cgctgcaatt tgtcgcaacg cccgtaattt gttcaaccgc atttgcctca
    20041 acggctacag ccagttctgc gggtaaatcc attgacaaca tctgtgcgat tttatgctgg
    20101 cttttctcat cccagcctaa ctgcgtgacg ccgcaccagt catgatcaaa tttaacgggt
    20161 aattggtcgt aaaaccgacg agcaaaagta aacgcattag agaaaaagcg gtttagcgcc
    20221 tcatcgtgtt tgcttaataa cggatacagc gccccctggc gattgccgga agcacccagc
    20281 gcgggggctt catccgcgca ataaagcgct acctgccagc cgcgccgtaa taacgccagc
    20341 gacaacaacg cgctggcaat accaccgccg ataatcgcta cttcccgttt gctgctgccc
    20401 gtacgattaa accacggcgt ggagcagggg agtggtaatg tctgttccat caccccacaa
    20461 agcatttccc gtttgcgccc aaagccctta cgtttttgca tcgtgaatcc ggcttcctgc
    20521 aaaccgcggc ggacaaaacc ggcagacgta aatgtcgcca gcgtgccgcc tggacgcgcc
    20581 aaccttgcca tggcgttaaa cagattttgc gtccacatat ccgggttttt agctggcgca
    20641 aagccgtcca gaaaccaggc atctactttt tgatttagcg aatcgtccag ttggctggtc
    20701 agttcgttaa tatcgccaaa ccataaatcc agcgtcacgc ggccttcatt gagcaataaa
    20761 cgatggcaac cgggcaaggg cattggccac tgcgcctgaa gttgttctgc ccacggagcc
    20821 agttccggcc agtgttgatg cgctaaggct aaatccgcac gggtgagggg aaatttctca
    20881 aaactaatga aatgtaagcg ttgtaattgc gcttgcggat gcgcttcgcg aaactgatca
    20941 aatgcctgcc atagcgtcag gaagtttaat ccggtgccga agccgctctc tgctaccaca
    21001 aacagaggat gtggatgctc aggaaagcgt acctctaatt ggttgcctcc cagaaaaaca
    21061 taacgcgtct cttccagccc gttatcgttg gaaaaataga catcgtcaaa atctcgggaa
    21121 acaggtgtac cctcagcatt aaattcgagg ttggcaggtt gtatggagta gtgtttcacg
    21181 taagttactc gtcttacagg cggtggctcg atcttagcga tgtgtgtaag gctgcgcaaa
    21241 tttctctatt aaatggctga tcggacttgt tcggcgtaca agtgtacgct attgtgcatt
    21301 cgaaacttac tctatgtgcg acttacagag gtattgaatg aaacgtgcag tgattactgg
    21361 cctgggcatt gtttccagca tcggtaataa ccagcaggaa gtcctggcat ctctgcgtga
    21421 aggacgttca gggatcactt tctctcagga gctgaaggat tccggcatgc gtagccacgt
    21481 ctggggcaac gtaaaactgg ataccactgg cctcattgac cgcaaagttg tgcgctttat
    21541 gagcgacgca tccatttatg cattcctttc tatggagcag gcaatcgcag atgcgggcct
    21601 ttctccggaa gcttaccaga ataacccgcg cgttggcctg attgcaggtt ctggcggcgg
    21661 ttccccgcgt ttccaggtat tcggcgctga cgcaatgcgc ggcccgcgcg gcctgaaagc
    21721 ggttggcccg tatgtggtca ccaaagcgat ggcatccggc gtttctgcct gcctcgccac
    21781 gccgtttaaa attcatggcg ttaactactc catcagctcc gcgtgtgcga cttccgcaca
    21841 ctgtatcggt aacgcagtag agcagatcca actgggcaaa caggacatcg tgtttgctgg
    21901 cggcggcgaa gagctgtgct gggaaatggc ctgcgaattc gacgcgatgg gtgcgctgtc
    21961 tactaaatac aacgacaccc cggaaaaagc ctcccgtact tacgacgctc accgtgacgg
    22021 tttcgttatc gctggcggcg gcggtatggt agtggttgaa gagctggaac acgcgctggc
    22081 gcgtggtgct cacatctatg ctgaaatcgt tggctacggc gcaacctctg atggtgcaga
    22141 catggttgct ccgtctggcg aaggcgcagt acgttgtatg cagatggcga tgcatggcgt
    22201 tgatacccca atcgattacc tgaactctca cggtacttcg actccggttg gcgacgtgaa
    22261 agagctggca gctatccgtg aagtgttcgg cgataagagc ccggcgattt ctgcaaccaa
    22321 agccatgacc ggtcactcac tgggcgctgc gggtgtgcag gaagctatct actctctgct
    22381 gatgctggaa cacggcttta tcgccccgag catcaacatt gaagagctgg acgagcaggc
    22441 tgcgggtctg aacatcgtga ccgaaacgac cgatcgcgaa ctgaccactg ttatgtctaa
    22501 cagcttcggc ttcggcggca ccaacgccac gctggtaatg cgcaagctga aagattaatt
    22561 cgcagtaggc cggagtaggt cggataagac gcgccagcgt cgcatccgac gttacgcgcc
    22621 aatgcggcct ccggcactaa cgcaaaaggg aacctgatgg ttcccttttt cacatcattg
    22681 acaatcgcca ccagttccag gcaaacttcc cgctttgtcg atttccttct gaaaagacgt
    22741 acgcgttaaa tcctgccaac gcaccgtaac cctgaaacca gagagatgag acggggatac
    22801 tcctcgcctt gcgctgcatt ctggagtaat gcatgactgc tgtaagccaa accgaaacac
    22861 gatcttctgc caatttttcg ctcttccgca tcgcttttgc ggtttttctc acctacatga
    22921 ccgtagggct gccgttgccg gttatcccgc tgtttgttca tcatgaactg ggctatggca
    22981 ataccatggt cggcattgcc gtcgggattc agtttctggc tacggtgctg acgcgtggct
    23041 atgccgggcg actggccgat caatatggcg caaaacgttc ggcgcttcag gggatgttag
    23101 cttgtggtct ggctggcggc gcgttgctgc tggcggcgat tttgcctgtc tccgcaccgt
    23161 tcaaatttgc cctgttggtc atcgggcgtt tgattcttgg gtttggtgaa agccagttac
    23221 tgacaggcgc tctgacctgg gggttaggca tcgtagggcc aaaacactct ggcaaagtga
    23281 tgtcatggaa tggaatggcg atttacggtg ccctcgctgt tggtgctccg cttggcctgt
    23341 tgattcatag ccattacggt tttgccgcac tggcgatcac cacaatggta ttacccttac
    23401 tggcgtgggc ctgtaacggc acagtgcgca aagtaccggc cctggcggga gaacgtccat
    23461 cgctgtggag cgttgtcggg tttatctgga aaccagggtt agggctggca ctacaaggcg
    23521 ttggttttgc ggttatcggg actttcgttt cgctctactt tgccagcaaa ggatgggcga
    23581 tggcgggctt tactcttacc gagtttggcg gcgcatttgt cgtgatgcgc gtcatgtttg
    23641 gctggatgcc ggaccgtttt ggcggcgtga aagtggcgat tgtctctctg cttgtagaaa
    23701 cggtgggctt gttgctgctc tggcaagccc caggtgcgtg ggtcgcatta gcgggcgcgg
    23761 cgttaaccgg agccggatgt tcgcttatct ttcctgcgct gggcgtggag gtggttaaac
    23821 gcgtcccctc acaagttcgc ggcaccgcac tgggcggtta cgccgcgttt caggatatcg
    23881 ccctcggcgt ctccgggccg ctggcgggaa tgctggcgac cacgtttggt tactcttcgg
    23941 tatttcttgc cggggcgatc tctgcggtgc tgggaattat tgtcacgata ctgtcatttc
    24001 gtcggggcta acgaaccagc cagaccagca tcaggataat cgcgactaac agtatccaca
    24061 gcgcgggacg cgttgccata ttttgcaacg tgtccatcat cggtggaaaa ggattaagca
    24121 gcggttgcat atcacgcgga tcaatccctt taacccgccg ttgataaagc tgattaagaa
    24181 tatcctcggc ctgggcagaa gaaagcgatg attgcgcaga aagaccatat ttttgctgga
    24241 tatacgccga taacgctgcc agttcactgg catctaaagg ttgttttagc gccatctgta
    24301 gtgattccag cgtcggcgta ttttgctggc ttaacgtctg gcgcgcctgc aaccaggtca
    24361 ccagatggtt aaacagtttc gctggaatta actcgccatc gttcaccccg gaaagttcca
    24421 gcatcgattg ccagatctgt ttgctgggtt cccccgttgc cgccgcaagt ttggtcacca
    24481 gctgttttag cgcattgtgc tccgccggta ataaaggacg gtcggtcgcc tcgcgctgct
    24541 gtggttgcgg aataaccatc ttcccttcct gcaacagggt gagaatggtt tttaattgct
    24601 ccggtgagag ctgattcagc ggcgtctgac caaagttatg acggatataa tccgtgaccg
    24661 cctgacgatt attccccaga cgtaaatact cccctaattg cgctaaaagc tggcgggcag
    24721 aatggctttt ttgcgcggcc agcagacgtt gcgccagatt atgctcagcg gcagggaagt
    24781 gacgcgaaag cagcggtgaa tctcccgaca gaccaatatc gtgcctgata cccgcccaca
    24841 gttctgctct ttgttgttgc gtcagtgagg tcactttcgt cattaagctt tccagcgaag
    24901 tacgttgctg actggataaa ggctgattgc ccgcgccaga cggcaggtta tctccctgac
    24961 ctggtggttg cccaggagga gggccggaaa taggttgtat cattacgtat ccttatacct
    25021 gaaatcttcg caagtatgcc tggccgcgag attatggcac acttgtccgg ttaactctcg
    25081 tctcatacag gtaacacaaa cgtgaaaatc cttgttgatg aaaatatgcc ttatgcccgc
    25141 gacttattta gccgtttggg tgaggtgacc gcggttcccg ggcgtccaat ccccgtcgct
    25201 caactggcag acgcggatgc gctgatggtg cgttcggtca cgaaagtgaa tgaatctttg
    25261 ctggcaggaa aacccattaa atttgttggc actgccactg cggggaccga ccatgtcgat
    25321 gaagcatggt tgaagcaggc gggaattggt ttttccgctg cacctggctg taatgcgatt
    25381 gcggtggtgg aatatgtttt ctcctcacta ctgatgcttg ccgaacgcga tggattttca
    25441 ctgcacgacc gtacggtggg gatcgtgggc gttggtaacg ttggacgtcg attgcaggcg
    25501 cgactggaag cgttagggat taaaacattg ctttgcgatc cgcctcgcgc tgaccgtggg
    25561 gatgagggtg atttccgctc gctggatgaa ttagttcagc atgcggatat tctgactttc
    25621 catacgccac tcttcaaaga tgggccgtac aaaacgctgc atctggcgga tgaaaaactg
    25681 atccgtagcc tgaagccggg agcgattctg attaacgcct gccgtggcgc agtcgtcgat
    25741 aatactgcgt tgctgacctg cctgaatgaa ggccagaagt taagcgtagt gctggatgtc
    25801 tgggaaggcg aaccggaact taacgtggag ttgctgacaa aagtggatat cggcacgccg
    25861 catatcgcag gttataccct ggaaggtaaa gcacgcggta ctacgcaagt gtttgaagcc
    25921 tatagcaagt ttattgggca tgaacagcac gttgcgctgg atacattact gcccgcgcca
    25981 gagtttggtc gcattacgct gcatggcccg ctcgatcaac cgacgctgaa aaggctggtg
    26041 catttggtgt atgatgtgcg ccgcgatgac gcaccgctgc gtaaagtcgc cgggataccg
    26101 ggtgagttcg ataaactgcg caaaaactat cttgagcgcc gtgaatggtc atctctgtat
    26161 gtaatttgtg atgacgccag tgcggcatca ttgctgtgta aactgggttt taacgccgtt
    26221 catcatccgg cacgttaatc tcttcttcat gctctctgct gtaacattgg cagggagctt
    26281 tgctatttct ggagtaaacc accatgtctg aaggctggaa cattgccgtc ctgggcgcaa
    26341 ctggcgctgt gggcgaagcc ctgcttgaaa cgctggctga acgtcagttc ccggttgggg
    26401 aaatttatgc actggcacgt aacgaaagcg caggcgaaca actgcgcttt ggtggtaaaa
    26461 caatcactgt gcaggatgcc gctgaattcg actggacgca ggcgcagctg gcattttttg
    26521 tcgcaggcaa agaagctacc gctgcctggg ttgaagaagc gaccaactca ggttgcctgg
    26581 tgatcgacag cagcggactg tttgctctcg aacccgacgt accgctggtg gtgccggaag
    26641 taaacccgtt tgtactgacc gattaccgca accggaatgt catcgccgta ccagacagtc
    26701 tgaccagcca gctgctggcg gcactgaaac cgttaatcga tcagggcggt ttgtcgcgta
    26761 tcagtgttac cagcctgatt tcagcctccg cccagggcaa aaaagcggtc gatgcgttag
    26821 cggggcagag tgcgaaattg ctcaacggca ttccgattga cgaagaagat ttcttcggtc
    26881 gtcaactggc gttcaatatg ctgccgttgt tgccggatag cgaaggtagc gtgcgtgaag
    26941 aacgtcgtat cgttgacgaa gtacgcaaaa tcctgcagga cgaagggctg atgatttcgg
    27001 caagcgtcgt ccaggcaccg gtattctacg gtcatgccca gatggtcaac tttgaagcac
    27061 tgcgtccgct ggcggcagaa gaagcgcgtg atgcgtttgc tcagggcgaa gatattgtgc
    27121 tctctgaaga gaacgaattc ccgactcagg taggggatgc ttcgggtacg ccgcatcttt
    27181 ctgttggctg cgtgcgtaat gactacggta tgccggagca agtccagttc tggtcggtgg
    27241 ccgataacgt tcgctttggc ggcgcgctga tggcagtaaa aatcgccgag aaactggtgc
    27301 aggagtatct gtactaatgt ccgaccagca acaactgcca gtttataaaa ttgcgctggg
    27361 cattgagtac gacggcagta agtattacgg ctggcaacgg cagaatgaag tccgcagtgt
    27421 gcaggagaag ctggaaaagg cgctctccca ggtggcgaac gaacccatca ccgtcttctg
    27481 cgccgggcgt actgacgcag gggtacacgg taccgggcag gtggtgcatt tcgaaacaac
    27541 cgcgcagcgc aaagacgcgg cgtggacctt gggcgtaaat gcgaatttac ctggtgacat
    27601 cgccgtgcgt tgggttaaag ctgtacctga tgattttcat gcccgattta gcgccacggc
    27661 tcgccgttat cgctacatca tctacaatca tcggctgcgc ccggcggtat tgagtaaagg
    27721 ggtaacccat ttttacgaac cgctggacgc tgaacggatg catcgggctg cgcaatgctt
    27781 gctgggcgag aatgatttca cctcgttccg tgcggtgcag tgccagtccc gaaccccgtg
    27841 gcgcaacgtt atgcatatta atgtcacgcg tcacggtcct tatgtggtgg tagatatcaa
    27901 agcgaatgcc tttgtacatc atatggtcag gaatattgtc ggcagcctga tggaagtcgg
    27961 tgcccacaac cagccggaga gatggatagc agagttgctg gcggcaaagg acagaacgct
    28021 ggcggcagca acggcaaaag cggaagggct gtatctggtc gcggtggatt accctgaccg
    28081 gtatgatctt ccaaaaccgc caatgggccc gctatttctg gcggactaac gagaataatg
    28141 ctcggaacat ttcaggctgt agccaacgta gttacagcct gaaagatgac gagtacaagg
    28201 cataggcaaa tatggacctg atttatttcc tcatcgattt tatcctgcac attgacgtgc
    28261 atctggcaga gctggtcgcg gagtacggcg tctgggttta tgccattctg tttttgattc
    28321 tgttctgcga aacggggctg gtggtaacgc cattcctgcc tggtgattcg ctgctgtttg
    28381 ttgctggtgc attggcgtcg ctggaaacta acgatctcaa tgtccatatg atggtggtgc
    28441 ttatgttgat tgccgctatt gtcggtgatg cagtgaacta taccattgga cggttatttg
    28501 gtgacaaact gttcagtaac ccggattcga aaatctttcg tcgcagttat cttgataaga
    28561 ctcatcagtt ttacgagaga catggcggca aaacgattat tctcgcccgt tttgtaccga
    28621 tcgtcagaac gttcgcgccg tttgttgcgg gaatgggcca catgtcgtac cgtcatttcg
    28681 ccgcttataa cgtgatcggc gcactgttgt gggtactgat ttttacctac gcgggttatt
    28741 tcttcggcac gctccccttc attcagagca accttaagct gatgattgtt ggcattattt
    28801 ttgtttcaat attgcctggg gttttcgaat ttattcgtca caaacgcgct gcggcacgcg
    28861 ccgcaaaata aaaagtaact ccgcggttcg accacttttt tatccaaagt ttcgggctgt
    28921 tatgttttaa tgtgcaacat tcatggtctg ttgggggcaa aaatggcatt atgcgtcccc
    28981 aaagataaaa ctggcatcga accaggttca gacagaaagg tccctaatga gctggattga
    29041 acgaattaaa agcaacatta ctcccacccg caaggcgagc attcctgaag gggtgtggac
    29101 taagtgtgat agctgcggtc aggttttata ccgcgctgag ctggaacgta atcttgaggt
    29161 ctgtccgaag tgtgaccatc acatgcgtat gacagcgcgt aatcgcctgc atagcctgtt
    29221 agatgaagga agccttgtgg agctgggtag cgagcttgag ccgaaagatg tgctgaagtt
    29281 tcgtgactcc aagaagtata aagaccgtct ggcttctgcg cagaaagaaa ccggcgaaaa
    29341 agatgcgctg gtggtgatga aaggcactct gtatggaatg ccggttgtcg ctgcggcatt
    29401 cgagttcgcc tttatgggcg gttcaatggg gtctgttgtg ggtgcacgtt tcgtgcgtgc
    29461 cgttgagcag gcgctggaag ataactgccc gctgatctgc ttctccgcct ctggtggcgc
    29521 acgtatgcag gaagcactga tgtcgctgat gcagatggcg aaaacctctg cggcactggc
    29581 aaaaatgcag gagcgcggct tgccgtacat ctccgtgctg accgacccga cgatgggcgg
    29641 tgtttctgca agtttcgcca tgctgggcga tctcaacatc gctgaaccga aagcgttaat
    29701 tggctttgcc ggtccgcgtg ttatcgaaca gaccgttcgc gaaaaactgc cgccaggatt
    29761 ccagcgcagt gaattcctga tcgagaaagg cgccatcgac atgatcgtcc atcgtccgga
    29821 aatgcgcctg aaactggcga gcattctggc gaagttgatg aatctgccag cgccgaatcc
    29881 tgaagcgccg cgtgaaggcg tagtggtacc cccggtaccg gatcaggaac ctgaggcctg
    29941 ataactgata agggcagggc cactggctct gcccttttgc tattctcacc gtaacgaatc
    30001 agcggatacc atgattatca aacgcactcc tcaagccgcg tcgcctctgg cttcgtggct
    30061 ttcttatctg gaaaacctgc acagtaaaac tatcgatctc ggccttgagc gcgtgagcca
    30121 ggtcgcggcg cgtcttggcg tcctgaaacc agcgccattt gtgtttaccg ttgcgggtac
    30181 gaatggcaaa ggcaccacct gccgtacgct ggagtcgatt ctgatggcgg cagggtacaa
    30241 agtgggcgtc tacagttcac cgcatctggt gcgttatacc gagcgcgtac gtgtgcaggg
    30301 gcaggaattg ccggaatcgg cccacaccgc ctcttttgcg gagattgaat cggcacgcgg
    30361 tgatatttcc ctgacctatt tcgagtacgg tacgctgtcg gcgttgtggc tgttcaaaca
    30421 ggcacaactt gacgtagtga ttctggaagt agggctgggc ggtcgtctgg acgcaaccaa
    30481 tattgtcgat gccgatgtgg ctgtagtaac cagcattgcg ctggatcata ccgactggct
    30541 gggaccagat cgcgaaagta ttggtcgcga gaaagcaggc atctttcgca gcgcaaaacc
    30601 ggcaattgtc ggtgagccgg aaatgccttc taccattgct gatgtggcgc aggaaaaagg
    30661 tgcactgtta caacgtcggg gcgttgagtg gaactattcc gtcaccgatc atgactgggc
    30721 gtttagcgat gctcacggca cgctggaaaa tctgccgttg ccgcttgtcc cgcaaccgaa
    30781 tgccgcaaca gcgctggcgg cactgcgtgc cagcgggctg gaagtcagtg aaaatgccat
    30841 tcgcgacggg attgccagcg caattttgcc gggacgtttc cagattgtga gcgagtcgcc
    30901 acgcgttatt tttgatgtcg cgcataatcc acatgcggcg gaatatctca ccgggcgtat
    30961 gaaagcgcta ccgaaaaacg ggcgcgtgct ggcggttatc ggtatgctac atgataaaga
    31021 tattgccgga actctggcct ggttgaaaag cgtggttgat gactggtatt gtgcgccact
    31081 ggaagggccg cgcggtgcca cggcagaaca actgcttgag catttgggta acggcaaatc
    31141 atttgatagc gttgcgcagg catgggatgc cgcaatggcg gacgctaaag cggaagacac
    31201 cgtgctggtg tgtggttctt tccacacggt cgcacatgtc atggaagtga ttgacgcgag
    31261 gagaagcggt ggcaagtaag tttcagaatc ggttagtggg cacgatcgtg ctggtggcgc
    31321 tgggggtgat tgtacttcca gggctgctgg acgggcagaa aaaacattat caggatgagt
    31381 ttgcggctat cccgctggtt ccaaaagcgg gcgatcgcga tgagcctgat atgatgccag
    31441 ccgccaccca ggcgttgccg acgcagccgc cggaaggcgc agcggaagag gtgcgggcag
    31501 gtgatgccgc agcaccgtcg ctcgatccgg ccactattgc agccaataac accgagtttg
    31561 aaccggaacc tgcaccggtc gccccaccga agccgaaacc ggtggagccg cctaaaccga
    31621 aggttgaagc accacctgcg ccgaagccag aaccgaagcc ggtcgtggaa gaaaaagcag
    31681 cgccaacggg taaagcctat gttgtgcaac tgggtgcgct gaaaaatgcc gataaagtga
    31741 atgagattgt cggtaagctg cgcggtgccg gttatcgggt ttatacgtcg ccatccacgc
    31801 cagtgcaggg taaaattacc cgtattctgg ttgggccgga tgcctcgaaa gataagctga
    31861 aaggttcgct gggtgagttg aagcaacttt ctggcttaag tggcgtggta atgggctata
    31921 cgccgaatta atacagtctt gcctgatgcg acgctggcgc gtcttatcag gcctgggcag
    31981 tgttagaacc gtaggtcgga taaggcgttt acgccgcatc cgacacgcat tgcacgatgc
    32041 cgcaaaggca taaaaagtcg atggcgttga atattttttc agcgccattt ttattgatgc
    32101 gtgggaagga aatccctacg caaacgtttt ctttttctgt tagaatgcgc cccgaacagg
    32161 atgacagggc gtaaaatcgt gggacacata tggtctggat tgattacgcc ataatcgcgg
    32221 tgattgcttt ttcctctctg gttagcctga tccgcggctt tgttcgtgaa gcgttatcgc
    32281 tggtgacatg gggttgtgct ttctttgttg ccagtcatta ctacacttac ctgtcagtct
    32341 ggtttacggg ctttgaagac gaactggttc gaaatggaat tgccatcgcg gtactgttta
    32401 tcgctaccct gattgttggt gctatcgtga acttcgtgat aggccagttg gtggagaaaa
    32461 cggggttgtc aggcaccgat cgggtgctgg gcgtctgttt cggtgcgttg cgcggtgtgt
    32521 tgattgttgc cgccattctc ttctttctcg actcctttac cggggtgtcg aaaagcgaag
    32581 actggagcaa atcacagctg atcccgcagt tcagttttat catcagatgg ttttttgatt
    32641 atctgcaaag ctcgtcaagt ttcttgccca gagcgtaagt gctctgagat gtggcttaac
    32701 gaggaaaaag acgtatgtgc ggtattgtcg gtatcgccgg tgttatgccg gttaaccagt
    32761 cgatttatga tgccttaacg gtgcttcagc atcgcggtca ggatgccgcc ggcatcatca
    32821 ccatagatgc caataactgc ttccgtttgc gtaaagcgaa cgggctggtg agcgatgtat
    32881 ttgaagctcg ccatatgcag cgtttgcagg gcaatatggg cattggtcat gtgcgttacc
    32941 ctacggctgg cagctccagc gcctctgaag cgcagccgtt ttacgttaac tccccgtatg
    33001 gcattacgct tgcccacaac ggcaatctga ccaacgctca cgagttgcgt aaaaaactgt
    33061 ttgaagaaaa acgccgccac atcaacacca cttccgactc ggaaattctg cttaatatct
    33121 tcgctagcga gctggacaac ttccgccact acccgctgga agccgacaat attttcgctg
    33181 ccattgctgc cacaaaccgc ttaatccgcg gcgcatatgc ctgtgtggcg atgattatcg
    33241 gccacggtat ggttgctttc cgcgatccaa acgggattcg tccgctggta ctgggaaaac
    33301 gtgatattga cgagaaccgt acagaatata tggtcgcttc cgaaagcgta gcgctcgata
    33361 cgctgggctt tgatttcctg cgtgacgtcg cgccgggcga agcgatttac atcactgaag
    33421 aagggcagtt gtttacccgt caatgtgctg acaatccggt cagcaatccg tgcctgtttg
    33481 agtatgtata ctttgctcgc ccggactcgt tcatcgacaa aatttccgtt tacagcgcgc
    33541 gtgtgaatat gggcacgaaa ctgggcgaga aaattgcccg cgaatgggaa gatctggata
    33601 tcgacgtggt gatcccgatc ccagaaacct cgtgtgatat cgcgctggaa attgctcgta
    33661 ttctgggcaa accgtaccgc cagggcttcg ttaaaaaccg ctatgttggc cgcaccttta
    33721 tcatgccggg ccagcagctg cgtcgtaagt ccgtgcgccg taaactgaat gccaaccgcg
    33781 ccgagttccg cgataaaaac gtcctgctgg tcgacgactc catcgtccgt ggcaccactt
    33841 ctgagcagat tatcgagatg gcacgcgaag ccggagcgaa gaaagtgtac ctcgcttctg
    33901 cggcaccgga aattcgcttc ccgaacgttt atggtattga tatgccgagc gccacggaac
    33961 tgatcgctca cggtcgcgaa gttgatgaaa ttcgccagat catcggtgct gacgggttga
    34021 ttttccagga tctgaacgat ctgatcgacg ccgttcgcgc tgaaaatccg gatatccagc
    34081 agtttgaatg ctcggtgttc aacggcgtct acgtcaccaa agatgttgat cagggctacc
    34141 tcgatttcct cgatacgtta cgtaatgatg acgccaaagc agtgcaacgt cagaacgaag
    34201 tggaaaatct cgaaatgcat aacgaaggat gatgccttcg ctgagggtgc cggtctggca
    34261 ccctgacttg caactcccgc cgaaatactg caaagtctgc ctgcaagtct gacagggcaa
    34321 ctatttatga aacgactcat tgtaggcatc agcggtgcca gcggcgcgat ttatggcgtg
    34381 cgcttattac aggttctgcg cgatgtcaca gatatcgaaa cgcatctggt gatgagccag
    34441 gcggcgcgcc agaccttatc cctcgaaacg gatttttccc tgcgcgaagt gcaggcatta
    34501 gctgatgtca cgcacgatgc gcgcgatatt tccgccagca tctcttccgg ttctttccag
    34561 acgctgggga tggtgatttt accctgttca atcaaaaccc tttccggcat tgtccatagc
    34621 tatactgatg gcttactgac ccgtgcggca gatgtggtgc tgaaagagcg tcgcccgttg
    34681 gtgctctgcg tgcgtgaaac accattgcac ttaggccatc tgcgtttaat gactcaggcg
    34741 gcagaaatcg gtgcggtgat tatgcctccc gttccggcgt tttatcatcg cccgcaatcc
    34801 cttgatgatg tgataaatca gacggttaat cgtgttcttg accagtttgc aataaccctt
    34861 cctgaagatc tctttgcccg ctggcagggc gcataactcc gctgttgccc tgtttcaggg
    34921 caattttgca accgcgatca aatcctcgac attttgtttc tgccattcaa tcgaaacgct
    34981 gcgattcaac cgctatacct gctatcttca acttcaggac aataatgcaa cgtcttatta
    35041 acatatttaa cgttgaatgt tactgttgtc gtcaagatgg cataagacct gcatgaaaga
    35101 gcctgcaaac acacaacaca atacacaaca taaaaaagcc attttcactt gagggttatg
    35161 tatgaagaag tcgattctcg ctctgtcttt gttagtcggt ctctccgcag cggcttccag
    35221 ctatgcggcg ctaccggaga cggtacgtat cggaaccgat accacctatg cgccgttctc
    35281 gtcgaaagat gctaaaggtg attttgttgg ctttgatatc gatctcggta acgagatgtg
    35341 caaacggatg caggtgaaat gtacctgggt tgccagtgac tttgacgcgc tgatcccctc
    35401 actgaaagcg aaaaaaatcg acgccattat ttcgtcgctt tccattaccg ataaacgtca
    35461 gcaggagatt gccttctccg acaagctgta cgccgcagat tctcgtttga ttgctgccaa
    35521 aggttcaccg attcagccaa cgctggattc actgaaaggt aaacatgttg gtgtgctgca
    35581 gggatcaacc caggaagctt acgctaacga gacctggcgt agcaaaggcg tggatgtggt
    35641 ggcctatgcc aaccaggatt tggtctattc cgatctggct gcaggacgtc tggatgctgc
    35701 gttacaagat gaagttgctg ccagcgaagg attcctcaag caacctgctg gtaaagcttt
    35761 cgcctttgct ggctcatcag taaaagacaa aaaatacttc ggtgatggca ccggtgtagg
    35821 gctacgtaaa gatgatgctg aactgacggc tgccttcaat aaggcgcttg gcgagctgcg
    35881 tcaggacggc acctacgaca agatggcgaa aaagtatttc gactttaatg tctacggtga
    35941 ctgatacgtc gctgggaagc tgtacctgat ggaatgatca tcatggtgca cgccaggttt
    36001 gttgcactat cgtggtgcat tgaaatgcat acttaagcat ttttaatgaa aaataatacg
    36061 tctaacgggg cggaatattt tgccttgatg gtcaatttta tggcacgata agtgtaacaa
    36121 acctgtaaat attccctata aaaagactgt cagttgagga cattatgaaa aaactggtgc
    36181 tatcgctctc tctggttctg gccttctcca gcgcaactgc ggcatttgct gcgattccgc
    36241 aaaacatccg catcggtacc gacccgacct atgcgccatt tgaatcaaaa aattcacaag
    36301 gcgaactggt tggcttcgat atcgatctgg caaaggaatt atgcaaacgc atcaatacgc
    36361 aatgtacgtt tgtcgaaaat ccgctggatg cgttaatccc gtccttaaaa gcgaagaaga
    36421 ttgacgccat catgtcatcg ctttccatta cggaaaaacg tcagcaagaa atagccttca
    36481 ccgacaaact gtacgctgcc gattctcgtt tggtggtggc gaaaaattct gacattcagc
    36541 cgacagtcga gtcgctgaaa ggcaaacggg taggcgtatt gcagggcacc acccaggaga
    36601 cgttcggtaa tgaacattgg gcaccaaaag gcattgaaat cgtctcgtat caggggcagg
    36661 acaacattta ttctgacctg actgccggac gtattgatgc cgcgttccag gatgaggtcg
    36721 ctgccagcga aggtttcctc aaacaacctg tcggtaaaga ttacaaattc ggtggcccgt
    36781 ctgttaaaga tgaaaaactg tttggcgtag ggaccggcat gggcctgcgt aaagaagata
    36841 acgaactgcg cgaagcactg aacaaagcct ttgccgaaat gcgcgctgac ggtacttacg
    36901 agaaattagc gaaaaagtac ttcgattttg atgtttatgg tggctaatcg ccatcggtga
    36961 aagaaagccc gctcccttcg ggtaaccgga gggagacaag aggcggtaat tcaccacaca
    37021 cgacaggaca ggcagcatgt tgtatgggtt ttcaggtgtt attttacagg gtgcgctcgt
    37081 cacgctggag ctggctatca gctctgtagt gctcgctgta atcatcggtt taattggcgc
    37141 tggcggtaag ctctcgcaaa atcggctttc ggggctgatt ttcgaagggt acaccacgct
    37201 gattcgtggc gtgccggact tagtgttgat gctgctgatt ttctacggtt tgcagattgc
    37261 gctaaacacg gtgacggagg cgatgggcgt cgggcagatt gatatcgatc cgatggtcgc
    37321 tggtattatc actctcggtt ttatctacgg tgcttatttt accgaaacgt tccgtggtgc
    37381 ttttatggca gtgccgaaag gacatataga ggcggcgacg gcgttcggtt ttactcgtgg
    37441 gcaagtgttt cggcggatca tgtttccggc gatgatgcgt tacgccctgc caggcattgg
    37501 caacaactgg caggtgatcc tcaaatctac cgcactggtt tcgttactcg gcctggaaga
    37561 tgtggtcaaa gccacccaac tggcaggcaa aagtacctgg gaaccgttct atttcgccat
    37621 cgtctgtggc gtgatttacc tggttttcac cactgtttcc aatggtgtgc tgctgttcct
    37681 tgagcgccgc tactccgtgg gtgtgaagag ggctgacctg tgatcgaaat cttacatgaa
    37741 tactggaaac cgctgctgtg gaccgacggt tatcgcttta ctggtgtggc gatcactctg
    37801 tggctgctta ttttgtcggt agtgataggc ggagtcctgg cgctgtttct ggcgattggt
    37861 cgtgtctcca gtaataaata catccagttt ccaatctggt tatttaccta tatttttcgc
    37921 ggtacgccgc tgtatgttca gttgctggtg ttctattccg gcatgtacac gcttgagatt
    37981 gttaagggaa ccgaattcct taacgctttc ttccgcagtg gcctgaactg taccgtgctg
    38041 gcgctgacgc ttaacacctg cgcttacact accgagattt ttgctggggc aatccgttcg
    38101 gttccgcatg gggaaattga agccgccaga gcctatggct tctcgacttt taaaatgtat
    38161 cgttgcatta ttttgccttc tgcgctgcgt attgcgttac cggcatacag caacgaagtg
    38221 atcctgatgc tgcactctac tgcgctggcg tttacggcca ctgtgccgga tctgctgaaa
    38281 atagcccgcg atattaacgc cgccacgtat caacctttta ccgccttcgg cattgccgcg
    38341 gtgctctatt taatcatctc ttatgtcctg atcagcctct ttcgcagagc ggaaaaacgc
    38401 tggttgcagc atgtgaaacc ttcttcaacg cactgagaat acgatgtccg agaataaatt
    38461 aaacgttatc gatttgcaca aacgctacgg cgaacatgaa gtgctgaaag gggtctcact
    38521 gcaagcgaat gccggagatg taataagcat catcggatcg tcgggatcgg ggaaaagtac
    38581 ctttctgcgc tgcattaact tcctcgaaaa accgagtgaa gggtcgatcg tggtcaatgg
    38641 ccagacgatc aatctggtgc gcgacaaaga cggtcaactc aaagtcgccg ataaaaatca
    38701 actgcgctta ctgcgcacac gcctgacgat ggtattccag cacttcaatc tctggagcca
    38761 tatgacggtg ctggaaaacg tcatggaagc gccgattcag gtgttgggcc tgagcaagca
    38821 ggaagcgcgc gagcgggcgg tgaagtatct ggcgaaagtc gggatagacg aacgtgcgca
    38881 ggggaaatat ccggtgcatc tttccggcgg tcagcaacag cgtgtttcta tcgcgcgggc
    38941 gctggcgatg gaaccggaag ttttactgtt tgatgaacct acctcggcgc tcgatcctga
    39001 actggtaggc gaagtgttac gtattatgca gcaactggca gaagagggga aaaccatggt
    39061 ggtagtgact cacgaaatgg gctttgctcg ccatgtttct actcacgtca ttttcctcca
    39121 tcaggggaaa atagaagaag agggcgcgcc ggagcagtta tttggcaacc cgcaaagccc
    39181 tcgtctgcaa cagttcctta agggatcgct gaaataattt cctctccggt ggctaacgct
    39241 gccggagtat tttcatgggg aatttgcgag tcagcttccg ctgaaatgta aaagttgcag
    39301 tccgctaaaa atacttctga attcgctgtc caaagataaa aactgcctgc tgcattgggt
    39361 gttaatcgtg gttattgtcg cgtcatctta aatttgaggg gtgacggaac gccatgacaa
    39421 tatcgacaac ttccacgccg catgatgcgg tatttaaatc ttttttacgc catccag
//
LOCUS       DAETQN010000024        39409 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.024, whole genome shotgun sequence.
ACCESSION   DAETQN010000024 DAETQN010000000
VERSION     DAETQN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.024"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..266
                     /locus_tag="K0W68_001294"
     CDS             <1..266
                     /locus_tag="K0W68_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBI3072905.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="K0W68_001295"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="K0W68_001295"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBI3072906.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="K0W68_001296"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="K0W68_001296"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBI3072907.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="K0W68_001297"
     CDS             complement(1570..3270)
                     /locus_tag="K0W68_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HBI3072908.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQEQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="K0W68_001298"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="K0W68_001298"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBI3072909.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="K0W68_001299"
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="K0W68_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HBI3072910.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4828..6600
                     /locus_tag="K0W68_001300"
     CDS             4828..6600
                     /locus_tag="K0W68_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBI3072911.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="K0W68_001301"
     CDS             6593..8374
                     /locus_tag="K0W68_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBI3072912.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="K0W68_001302"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="K0W68_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBI3072913.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="K0W68_001303"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="K0W68_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBI3072914.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="K0W68_001304"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="K0W68_001304"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBI3072915.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="K0W68_001305"
     CDS             11336..11908
                     /locus_tag="K0W68_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBI3072916.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="K0W68_001306"
     CDS             complement(11939..12250)
                     /locus_tag="K0W68_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBI3072917.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="K0W68_001307"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="K0W68_001307"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="K0W68_001308"
     CDS             12629..12982
                     /locus_tag="K0W68_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBI3072918.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="K0W68_001309"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="K0W68_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="K0W68_001310"
     CDS             complement(14736..15206)
                     /locus_tag="K0W68_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HBI3072919.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="K0W68_001311"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="K0W68_001311"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBI3072920.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /gene="hha"
                     /locus_tag="K0W68_001312"
     CDS             complement(16044..16262)
                     /gene="hha"
                     /locus_tag="K0W68_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBI3072921.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="K0W68_001313"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="K0W68_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBI3072922.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17208..20357)
                     /locus_tag="K0W68_001314"
     CDS             complement(17208..20357)
                     /locus_tag="K0W68_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBI3072923.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="K0W68_001315"
     CDS             complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="K0W68_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBI3072924.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21715..22362
                     /gene="acrR"
                     /locus_tag="K0W68_001316"
     CDS             21715..22362
                     /gene="acrR"
                     /locus_tag="K0W68_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBI3072925.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22490..25852
                     /gene="mscK"
                     /locus_tag="K0W68_001317"
     CDS             22490..25852
                     /gene="mscK"
                     /locus_tag="K0W68_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBI3072926.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="K0W68_001318"
     CDS             complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="K0W68_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBI3072927.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(26239..26766)
                     /gene="priC"
                     /locus_tag="K0W68_001319"
     CDS             complement(26239..26766)
                     /gene="priC"
                     /locus_tag="K0W68_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBI3072928.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26836..27213
                     /locus_tag="K0W68_001320"
     CDS             26836..27213
                     /locus_tag="K0W68_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBI3072929.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27366..27917
                     /gene="apt"
                     /locus_tag="K0W68_001321"
     CDS             27366..27917
                     /gene="apt"
                     /locus_tag="K0W68_001321"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBI3072930.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28046..29971
                     /gene="dnaX"
                     /locus_tag="K0W68_001322"
     CDS             28046..29971
                     /gene="dnaX"
                     /locus_tag="K0W68_001322"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBI3072931.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29304..29368
                     /gene="dnaX"
                     /locus_tag="K0W68_001322"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30024..30353
                     /locus_tag="K0W68_001323"
     CDS             30024..30353
                     /locus_tag="K0W68_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBI3072932.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30353..30958
                     /gene="recR"
                     /locus_tag="K0W68_001324"
     CDS             30353..30958
                     /gene="recR"
                     /locus_tag="K0W68_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBI3072933.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31068..32942
                     /gene="htpG"
                     /locus_tag="K0W68_001325"
     CDS             31068..32942
                     /gene="htpG"
                     /locus_tag="K0W68_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBI3072934.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33123..33767
                     /gene="adk"
                     /locus_tag="K0W68_001326"
     CDS             33123..33767
                     /gene="adk"
                     /locus_tag="K0W68_001326"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBI3072935.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33899..34861
                     /gene="hemH"
                     /locus_tag="K0W68_001327"
     CDS             33899..34861
                     /gene="hemH"
                     /locus_tag="K0W68_001327"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBI3072936.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34858..35817)
                     /gene="aes"
                     /locus_tag="K0W68_001328"
     CDS             complement(34858..35817)
                     /gene="aes"
                     /locus_tag="K0W68_001328"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HBI3072937.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35969..37273
                     /gene="gsk"
                     /locus_tag="K0W68_001329"
     CDS             35969..37273
                     /gene="gsk"
                     /locus_tag="K0W68_001329"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBI3072938.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37406..39082)
                     /locus_tag="K0W68_001330"
     CDS             complement(37406..39082)
                     /locus_tag="K0W68_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBI3072939.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         9526 a        10049 c        10417 g         9417 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 tcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc tcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtctcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat ggattattaa atctattagc
    17041 gctacttaat ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat
    17101 actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt
    17161 atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg
    17221 acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca
    17281 aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca
    17341 ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata
    17401 acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca
    17461 tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg
    17521 acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag
    17581 taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc
    17641 ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac
    17701 aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt
    17761 tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt
    17821 tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt
    17881 tccatggatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa
    17941 gaggagaacg ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg
    18001 atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga
    18061 ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta
    18121 atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac
    18181 tgcggggtat cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct
    18241 gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg
    18301 tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta
    18361 aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc
    18421 gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca
    18481 ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca
    18541 acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta
    18601 cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca
    18661 tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc
    18721 agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag
    18781 tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc
    18841 ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc
    18901 aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag
    18961 atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc
    19021 gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc
    19081 ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc
    19141 accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca
    19201 aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc
    19261 aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct
    19321 tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac
    19381 gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg
    19441 attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa
    19501 gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc
    19561 gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg
    19621 aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct
    19681 ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg
    19741 gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag
    19801 atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg
    19861 atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca
    19921 tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc
    19981 ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc
    20041 tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta
    20101 cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt
    20161 tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg
    20221 gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc
    20281 gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg
    20341 ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg
    20401 ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg
    20461 gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc
    20521 ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac
    20581 tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc
    20641 ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa
    20701 catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc
    20761 agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat
    20821 gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt
    20881 cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat
    20941 cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc
    21001 ttccgtcacg ttcgatttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc
    21061 cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg
    21121 ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag
    21181 tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag
    21241 atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga
    21301 gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac
    21361 ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat
    21421 ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg
    21481 ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac
    21541 cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa
    21601 attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat
    21661 ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca
    21721 cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt
    21781 cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc
    21841 gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc
    21901 tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct
    21961 ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca
    22021 gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa
    22081 atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa
    22141 caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg
    22201 gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg
    22261 caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat
    22321 ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag
    22381 gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc
    22441 ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt
    22501 ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt
    22561 cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa
    22621 agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga
    22681 caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat
    22741 aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccggaaa aaatgcgcca
    22801 ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat
    22861 tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt
    22921 gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca
    22981 gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag
    23041 tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat
    23101 gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg
    23161 gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc
    23221 ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt
    23281 aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa
    23341 tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc
    23401 gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg
    23461 ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct
    23521 gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa
    23581 catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga
    23641 tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt
    23701 caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga
    23761 tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca
    23821 gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg
    23881 ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa
    23941 agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt
    24001 tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct
    24061 gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga
    24121 tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg
    24181 cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact
    24241 gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa
    24301 ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag
    24361 ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc
    24421 tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat
    24481 tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg
    24541 tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat
    24601 tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg
    24661 ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg
    24721 cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa
    24781 tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga
    24841 acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt
    24901 catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac
    24961 gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg
    25021 cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc
    25081 tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc
    25141 cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc
    25201 gctgggcgtc tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg
    25261 ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg
    25321 tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat
    25381 ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa
    25441 agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt
    25501 gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa
    25561 ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc
    25621 gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg
    25681 tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat
    25741 caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac
    25801 ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa
    25861 gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg
    25921 cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg
    25981 tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga
    26041 catttagcgg ccccatttat ttctcacttt tccgccttat catcgcgcgt taatttcttt
    26101 tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt
    26161 tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt
    26221 gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag
    26281 cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg
    26341 ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg
    26401 tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg
    26461 tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag
    26521 ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc
    26581 atgacaaagc gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc
    26641 acgagtctga aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg
    26701 ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt
    26761 tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag
    26821 ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg
    26881 ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg
    26941 gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg
    27001 tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg
    27061 cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca
    27121 tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt
    27181 ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt
    27241 gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac
    27301 aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata
    27361 cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc
    27421 aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga
    27481 aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca
    27541 aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg
    27601 gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa
    27661 cttacgacct ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg
    27721 gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg
    27781 ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt
    27841 tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc
    27901 cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga
    27961 tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca
    28021 ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc
    28081 acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt
    28141 gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac
    28201 ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc
    28261 gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga
    28321 aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca
    28381 gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc
    28441 gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt
    28501 tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca
    28561 atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa
    28621 cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg
    28681 cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt
    28741 ttcaacccag gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct
    28801 ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc
    28861 tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat
    28921 tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca ttgagctgcg
    28981 gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct
    29041 gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac
    29101 gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg
    29161 ccagtccttt gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca
    29221 atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc
    29281 ggcgcgccag cagttgcagt gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc
    29341 agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac
    29401 cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga
    29461 agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc
    29521 gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc
    29581 ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa
    29641 actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct
    29701 gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga
    29761 agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc
    29821 cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc
    29881 gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga
    29941 gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct
    30001 taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc
    30061 aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag
    30121 tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc
    30181 gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg
    30241 tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg
    30301 cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac
    30361 cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa
    30421 gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt
    30481 ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt
    30541 caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat
    30601 ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg
    30661 tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat
    30721 cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc
    30781 caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca
    30841 atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat
    30901 ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc
    30961 aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc
    31021 ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag
    31081 aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct
    31141 attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca
    31201 agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg
    31261 ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga
    31321 tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat
    31381 tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg
    31441 ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag
    31501 gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg
    31561 ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag
    31621 acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg
    31681 cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg
    31741 agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag
    31801 agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc
    31861 acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc
    31921 cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt
    31981 tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga
    32041 ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa
    32101 cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga
    32161 aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag
    32221 gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta
    32281 cccataccga ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag
    32341 aagggcagga gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc
    32401 cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg
    32461 atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta
    32521 aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg
    32581 agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag
    32641 atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg
    32701 aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga
    32761 aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag
    32821 atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac
    32881 gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct
    32941 gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt
    33001 ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc
    33061 tgttggtggt atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg
    33121 caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca
    33181 tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca
    33241 aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca
    33301 ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg
    33361 gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg
    33421 gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta
    33481 tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc
    33541 cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc
    33601 aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg
    33661 gctactactt caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca
    33721 agtcggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca
    33781 gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt
    33841 tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat
    33901 gcgtcagact aaaatcggta tcctgctggc aaacctgggt acgcccgatg cccccacacc
    33961 tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc
    34021 acggttgtta tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt
    34081 ggcgaagctg tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg
    34141 ccagcaacag caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag
    34201 ctacggctcg ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca
    34261 tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga
    34321 tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga
    34381 ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc
    34441 caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc
    34501 agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc
    34561 actggggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc
    34621 ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca
    34681 tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc
    34741 cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc
    34801 gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta
    34861 aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat
    34921 ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg
    34981 ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc
    35041 cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta
    35101 atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc
    35161 ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa
    35221 tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc
    35281 acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc
    35341 aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc
    35401 cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc
    35461 aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag
    35521 attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg
    35581 cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt
    35641 tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg
    35701 ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac
    35761 ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca
    35821 aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc
    35881 tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc
    35941 gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca
    36001 ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac
    36061 cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga
    36121 tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga
    36181 cgtagccgaa gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc
    36241 gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt
    36301 gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg
    36361 taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg
    36421 ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat
    36481 gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt
    36541 tctcacctct tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa
    36601 agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt
    36661 tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct
    36721 tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc
    36781 tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta
    36841 tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg
    36901 cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca
    36961 gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat
    37021 gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa
    37081 cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta
    37141 ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca
    37201 gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta
    37261 ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg
    37321 catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc
    37381 atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt
    37441 ccagcagttc cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc
    37501 cacgttcggt gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat
    37561 ccgggttttt cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg
    37621 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg
    37681 cattgcccaa tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg
    37741 tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta
    37801 cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt
    37861 cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    37921 ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca
    37981 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    38041 cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac
    38101 caaacagacg gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg
    38161 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    38221 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    38281 gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga
    38341 cgtcaaacaa ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    38401 taaatagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc
    38461 gacctaccag catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat
    38521 cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca
    38581 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    38641 gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    38701 tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg
    38761 cagagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg
    38821 cgatggcctt taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca
    38881 acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    38941 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    39001 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    39061 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    39121 ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa
    39181 taattattga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac
    39241 agaaggcggt ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt
    39301 gcctttggct gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca
    39361 tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatgct
//
LOCUS       DAETQN010000025        39262 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.025, whole genome shotgun sequence.
ACCESSION   DAETQN010000025 DAETQN010000000
VERSION     DAETQN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.025"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="K0W68_001331"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="K0W68_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HBI3072940.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="K0W68_001332"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="K0W68_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBI3072941.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="K0W68_001333"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="K0W68_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBI3072942.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="K0W68_001334"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="K0W68_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBI3072943.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="K0W68_001335"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="K0W68_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBI3072944.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="K0W68_001336"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="K0W68_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBI3072945.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="K0W68_001337"
     CDS             complement(6574..7338)
                     /locus_tag="K0W68_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3072946.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="K0W68_001338"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="K0W68_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBI3072947.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="K0W68_001339"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="K0W68_001339"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBI3072948.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="K0W68_001340"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="K0W68_001340"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBI3072949.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="K0W68_001341"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="K0W68_001341"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBI3072950.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="K0W68_001342"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="K0W68_001342"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBI3072951.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="K0W68_001343"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="K0W68_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBI3072952.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="K0W68_001344"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="K0W68_001344"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBI3072953.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="K0W68_001345"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="K0W68_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBI3072954.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="K0W68_001346"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="K0W68_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBI3072955.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="K0W68_001347"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="K0W68_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBI3072956.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="K0W68_001348"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="K0W68_001348"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBI3072957.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALREYVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="K0W68_001349"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="K0W68_001349"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBI3072958.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="K0W68_001350"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="K0W68_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBI3072959.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="K0W68_001351"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="K0W68_001351"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBI3072960.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="K0W68_001352"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="K0W68_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBI3072961.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="K0W68_001353"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="K0W68_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBI3072962.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="K0W68_001354"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="K0W68_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBI3072963.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="K0W68_001355"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="K0W68_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="K0W68_001356"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="K0W68_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBI3072964.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="K0W68_001357"
     CDS             complement(31717..33003)
                     /locus_tag="K0W68_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI3072965.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="K0W68_001358"
     CDS             complement(33054..33995)
                     /locus_tag="K0W68_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBI3072966.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="K0W68_001359"
     CDS             complement(34010..34780)
                     /locus_tag="K0W68_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBI3072967.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35253..36767
                     /gene="caiT"
                     /locus_tag="K0W68_001360"
                     /pseudo
     CDS             35253..36767
                     /gene="caiT"
                     /locus_tag="K0W68_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
     gene            36798..37940
                     /gene="caiA"
                     /locus_tag="K0W68_001361"
     CDS             36798..37940
                     /gene="caiA"
                     /locus_tag="K0W68_001361"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBI3072968.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            38069..39262
                     /gene="caiB"
                     /locus_tag="K0W68_001362"
                     /pseudo
     CDS             38069..39262
                     /gene="caiB"
                     /locus_tag="K0W68_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT         9400 a        10790 c        10279 g         8793 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tatgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcctg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaatgt
    35101 gataacaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    35161 cacgaggtta ataataatta tatcaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    35221 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    35281 tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg catactttgt tggcttacag
    35341 tcagagatct ggatgcagcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    35401 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    35461 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc acctgaattt agtaccgcca
    35521 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    35581 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacagggg
    35641 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    35701 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    35761 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggca
    35821 ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    35881 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    35941 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    36001 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    36061 tgggttgggt gttcattgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    36121 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    36181 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tatgctatcc
    36241 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    36301 gcatggtgct ggggctgaca gcgtcaacct ggatcctgtg gactgtactc ggtagtaaca
    36361 ctctgctgtt gatagataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    36421 tggcgcgcgc catcattgaa acctaggccg ctctgccact cagcaccgcc accatgtggg
    36481 gcttcttcat cctctgcttt attgccaccg ttacactggt taacgcctgc tcttataccc
    36541 tggcgatgtc cacttgccgc gaagtacgcg atggtgaaga accacctctg ctggtgcgta
    36601 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    36661 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    36721 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    36781 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    36841 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    36901 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    36961 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    37021 tggagctggg acgtctgggg gcaccaacct atgtgctgta ccagttgccg ggcgggttca
    37081 acaccttcct gcgcgaaggc acacaagagc agatcgacaa aattatggct ttccgcggca
    37141 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    37201 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    37261 ttattaccag cagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    37321 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    37381 ccaaacttga aaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgacg
    37441 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    37501 aagagttcga ccatgaacgt ttcctggtag ccctcaccaa ctacggtacg gcgatgtgcg
    37561 cttttgaaga tgcggcgcgc tacgccaatc agcgcgtgca gtttggcgag gctattggtc
    37621 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    37681 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    37741 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt tgtggatagc gcaatgcagg
    37801 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgatctgc
    37861 gtgtagaccg cgtctccggg ggatctgacg aaatgcagat cctgacgctg ggtcgcgcgg
    37921 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctaatg cgtcttatca
    37981 ggcctacggg agcacgaatg taggccggat aaagcgttta cgccgcatcc ggcagtcatg
    38041 cactacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcgggcc
    38101 gttggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccgggccgt tcgccgggca
    38161 aatgttcgca gaatggggtg cggaagttat ctggatcgag aacgtcgcct aggccgacac
    38221 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    38281 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    38341 cttcatcgaa gccagcaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    38401 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    38461 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    38521 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    38581 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    38641 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    38701 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    38761 ctactacgcc ggttgcggtc tgtataaatg cgccgacggc tacatcgtga tggaactggt
    38821 gggcattacc caaattgaag agtgctttaa agatattgac ctcgcacatc tactcggtac
    38881 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    38941 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    39001 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    39061 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    39121 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    39181 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    39241 cattcaggag ttggtcagca aa
//
LOCUS       DAETQN010000026        38337 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.026, whole genome shotgun sequence.
ACCESSION   DAETQN010000026 DAETQN010000000
VERSION     DAETQN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.026"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            59..1423
                     /locus_tag="K0W68_001363"
     CDS             59..1423
                     /locus_tag="K0W68_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3072969.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="K0W68_001364"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="K0W68_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBI3072970.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="K0W68_001365"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="K0W68_001365"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBI3072971.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="K0W68_001366"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="K0W68_001366"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBI3072972.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="K0W68_001367"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="K0W68_001367"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBI3072973.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="K0W68_001368"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="K0W68_001368"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBI3072974.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="K0W68_001369"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="K0W68_001369"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBI3072975.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="K0W68_001370"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="K0W68_001370"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBI3072976.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="K0W68_001371"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="K0W68_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBI3072977.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="K0W68_001372"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="K0W68_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBI3072978.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="K0W68_001373"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="K0W68_001373"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBI3072979.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="K0W68_001374"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="K0W68_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBI3072980.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="K0W68_001375"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="K0W68_001375"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBI3072981.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="K0W68_001376"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="K0W68_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBI3072982.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="K0W68_001377"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="K0W68_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBI3072983.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="K0W68_001378"
     CDS             13749..14288
                     /locus_tag="K0W68_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBI3072984.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="K0W68_001379"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="K0W68_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBI3072985.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /locus_tag="K0W68_001380"
     CDS             complement(15648..15995)
                     /locus_tag="K0W68_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBI3072986.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="K0W68_001381"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="K0W68_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBI3072987.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="K0W68_001382"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="K0W68_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBI3072988.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="K0W68_001383"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="K0W68_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBI3072989.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="K0W68_001384"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="K0W68_001384"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBI3072990.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="K0W68_001385"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="K0W68_001385"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBI3072991.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="K0W68_001386"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="K0W68_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HBI3072992.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="K0W68_001387"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="K0W68_001387"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBI3072993.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="K0W68_001388"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="K0W68_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBI3072994.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="K0W68_001389"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="K0W68_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBI3072995.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="K0W68_001390"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="K0W68_001390"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBI3072996.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="K0W68_001391"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="K0W68_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HBI3072997.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="K0W68_001392"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="K0W68_001392"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBI3072998.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="K0W68_001393"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="K0W68_001393"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBI3072999.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="K0W68_001394"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="K0W68_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBI3073000.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="K0W68_001395"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="K0W68_001395"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBI3073001.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="K0W68_001396"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="K0W68_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBI3073002.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="K0W68_001397"
     CDS             complement(37465..37920)
                     /locus_tag="K0W68_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073003.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="K0W68_001398"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="K0W68_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9471 a        10314 c         9728 g         8824 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaaacccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacac ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactac cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat caacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cactcccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcca
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc ccccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctatcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctagcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcacgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgcctggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacaa cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ctgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccaccag tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaggcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggat gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAETQN010000027        37810 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.027, whole genome shotgun sequence.
ACCESSION   DAETQN010000027 DAETQN010000000
VERSION     DAETQN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37810
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.027"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(269..886)
                     /gene="tdk"
                     /locus_tag="K0W68_001399"
     CDS             complement(269..886)
                     /gene="tdk"
                     /locus_tag="K0W68_001399"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBI3073004.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1491..1904
                     /gene="hns"
                     /locus_tag="K0W68_001400"
     CDS             1491..1904
                     /gene="hns"
                     /locus_tag="K0W68_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBI3073005.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2048..2956)
                     /gene="galU"
                     /locus_tag="K0W68_001401"
     CDS             complement(2048..2956)
                     /gene="galU"
                     /locus_tag="K0W68_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBI3073006.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="K0W68_001402"
     CDS             complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="K0W68_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBI3073007.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="K0W68_001403"
     CDS             complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="K0W68_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBI3073008.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5281..5739
                     /locus_tag="K0W68_001404"
     CDS             5281..5739
                     /locus_tag="K0W68_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBI3073009.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5789..6631
                     /gene="purU"
                     /locus_tag="K0W68_001405"
     CDS             5789..6631
                     /gene="purU"
                     /locus_tag="K0W68_001405"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBI3073010.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6791..6875
                     /locus_tag="K0W68_001406"
     tRNA            6791..6875
                     /locus_tag="K0W68_001406"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6825..6827,aa:Tyr,seq:gta)
     gene            6923..7054
                     /locus_tag="K0W68_001407"
     ncRNA           6923..7054
                     /ncRNA_class="other"
                     /locus_tag="K0W68_001407"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7238..7915)
                     /gene="narI"
                     /locus_tag="K0W68_001408"
     CDS             complement(7238..7915)
                     /gene="narI"
                     /locus_tag="K0W68_001408"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBI3073011.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7915..8625)
                     /gene="narJ"
                     /locus_tag="K0W68_001409"
     CDS             complement(7915..8625)
                     /gene="narJ"
                     /locus_tag="K0W68_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBI3073012.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8622..10160)
                     /gene="narH"
                     /locus_tag="K0W68_001410"
     CDS             complement(8622..10160)
                     /gene="narH"
                     /locus_tag="K0W68_001410"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBI3073013.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10157..13900)
                     /locus_tag="K0W68_001411"
     CDS             complement(10157..13900)
                     /locus_tag="K0W68_001411"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBI3073014.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14293..15684)
                     /gene="narK"
                     /locus_tag="K0W68_001412"
     CDS             complement(14293..15684)
                     /gene="narK"
                     /locus_tag="K0W68_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBI3073015.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16023..17819
                     /gene="narX"
                     /locus_tag="K0W68_001413"
     CDS             16023..17819
                     /gene="narX"
                     /locus_tag="K0W68_001413"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBI3073016.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17812..18462
                     /gene="narL"
                     /locus_tag="K0W68_001414"
     CDS             17812..18462
                     /gene="narL"
                     /locus_tag="K0W68_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBI3073017.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18463..19857)
                     /gene="ychO"
                     /locus_tag="K0W68_001415"
     CDS             complement(18463..19857)
                     /gene="ychO"
                     /locus_tag="K0W68_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBI3073018.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20042..20395
                     /gene="ychN"
                     /locus_tag="K0W68_001416"
     CDS             20042..20395
                     /gene="ychN"
                     /locus_tag="K0W68_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBI3073019.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20439..21134)
                     /gene="chaC"
                     /locus_tag="K0W68_001417"
     CDS             complement(20439..21134)
                     /gene="chaC"
                     /locus_tag="K0W68_001417"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBI3073020.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21292..21522)
                     /gene="chaB"
                     /locus_tag="K0W68_001418"
     CDS             complement(21292..21522)
                     /gene="chaB"
                     /locus_tag="K0W68_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBI3073021.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21792..22892
                     /gene="chaA"
                     /locus_tag="K0W68_001419"
     CDS             21792..22892
                     /gene="chaA"
                     /locus_tag="K0W68_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBI3073022.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23295..23402
                     /locus_tag="K0W68_001420"
     CDS             23295..23402
                     /locus_tag="K0W68_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI3073023.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            23831..23938
                     /locus_tag="K0W68_001421"
     CDS             23831..23938
                     /locus_tag="K0W68_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI3073024.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            24366..24473
                     /locus_tag="K0W68_001422"
     CDS             24366..24473
                     /locus_tag="K0W68_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI3073025.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(24622..25476)
                     /gene="kdsA"
                     /locus_tag="K0W68_001423"
     CDS             complement(24622..25476)
                     /gene="kdsA"
                     /locus_tag="K0W68_001423"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBI3073026.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(25512..26320)
                     /locus_tag="K0W68_001424"
                     /pseudo
     CDS             complement(25512..26320)
                     /locus_tag="K0W68_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            complement(26324..26716)
                     /locus_tag="K0W68_001425"
     CDS             complement(26324..26716)
                     /locus_tag="K0W68_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBI3073027.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(26713..27546)
                     /gene="prmC"
                     /locus_tag="K0W68_001426"
     CDS             complement(26713..27546)
                     /gene="prmC"
                     /locus_tag="K0W68_001426"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBI3073028.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(27546..28628)
                     /gene="prfA"
                     /locus_tag="K0W68_001427"
     CDS             complement(27546..28628)
                     /gene="prfA"
                     /locus_tag="K0W68_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBI3073029.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(28670..29926)
                     /gene="hemA"
                     /locus_tag="K0W68_001428"
     CDS             complement(28670..29926)
                     /gene="hemA"
                     /locus_tag="K0W68_001428"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBI3073030.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            30140..30763
                     /gene="lolB"
                     /locus_tag="K0W68_001429"
     CDS             30140..30763
                     /gene="lolB"
                     /locus_tag="K0W68_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBI3073031.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            30763..31614
                     /gene="ispE"
                     /locus_tag="K0W68_001430"
     CDS             30763..31614
                     /gene="ispE"
                     /locus_tag="K0W68_001430"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBI3073032.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            31765..32712
                     /gene="prs"
                     /locus_tag="K0W68_001431"
     CDS             31765..32712
                     /gene="prs"
                     /locus_tag="K0W68_001431"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBI3073033.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            32836..34515
                     /gene="dauA"
                     /locus_tag="K0W68_001432"
     CDS             32836..34515
                     /gene="dauA"
                     /locus_tag="K0W68_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBI3073034.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(34570..34848)
                     /gene="ychH"
                     /locus_tag="K0W68_001433"
     CDS             complement(34570..34848)
                     /gene="ychH"
                     /locus_tag="K0W68_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBI3073035.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            35125..35709
                     /gene="pth"
                     /locus_tag="K0W68_001434"
     CDS             35125..35709
                     /gene="pth"
                     /locus_tag="K0W68_001434"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBI3073036.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            35826..36917
                     /gene="ychF"
                     /locus_tag="K0W68_001435"
     CDS             35826..36917
                     /gene="ychF"
                     /locus_tag="K0W68_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBI3073037.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         9411 a         9790 c         9241 g         9368 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgct tgatcaagac
      421 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
      481 cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta
      541 aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt
      601 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
      661 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
      721 acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat
      781 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
      841 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
      901 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
      961 agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga
     1021 gctatcatta caactgcctc gcttgttata agcgggtaaa tgactactgg taactattca
     1081 caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat
     1141 actaagtcca tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt
     1201 ctcgtaaaca caactaatac agaagactga aaggtcgtca gcctacgata atctccccat
     1261 aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat
     1321 agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca
     1381 aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc
     1441 tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag
     1501 cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg
     1561 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taacgaacgt cgcgaagaag
     1621 aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa
     1681 tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat
     1741 ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg
     1801 gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg
     1861 atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca
     1921 cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata
     1981 agaataagct ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat
     2041 acgaatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa
     2101 gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc
     2161 agtcatggct cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat
     2221 caattgcgtc ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg
     2281 gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat
     2341 ccgctttcgg tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa
     2401 cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct
     2461 ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat
     2521 cggattcata ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca
     2581 ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt
     2641 gcataatagt cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta
     2701 cacgtttttc cagcattgct tccagttcaa aactggtatc aaagtggttt tcaatagagt
     2761 ttttagatga gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca
     2821 cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg
     2881 tcgccggcaa catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg
     2941 tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt
     3001 ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag
     3061 ttagcataat taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt
     3121 agcaggtaat gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc
     3181 gcagtcgacc accggttccc catatttggc attgccaggc atcgcatcgc tggctcaatt
     3241 gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt
     3301 gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt
     3361 cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc tgacggagta
     3421 aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa
     3481 gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat
     3541 tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg
     3601 caatatcaag caccaggccg ggtttgtccg cggcaaccaa ttgacgataa ttaacccggc
     3661 aatgggaaat cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg
     3721 cagggttatc aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg
     3781 aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt
     3841 taactggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca
     3901 tattttcagt ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct
     3961 ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag
     4021 tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc
     4081 ccaatgagga aaaccatgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga
     4141 gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgactggc tttactcaat
     4201 agtggcatgc tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct
     4261 ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcctttc cactgccagc
     4321 tgtccggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa
     4381 acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc
     4441 tccaacacct ggatagacgt ggtcataatc tctgttgccg ttggcgctgt cacagcccga
     4501 cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggtag agaatcacca
     4561 ttctcgctat tttcttcact gacattaaag gagagcagat cttgttgcat caaatgagca
     4621 tcgtgctgca ggtcgaccgc tatcacaata tcagccccca atgcacgcgt aagagaaata
     4681 ggaattgggt taactactgc cccatcaacc agccagtagc cgttatgtgc aacgggtgcc
     4741 atgagtcctg gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac
     4801 cataattcac gccccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt
     4861 tcgatctctg tttccggcat tatttcgcga tattgattga agacacgctc gccgcgcagt
     4921 aacccaccgc gctgccagga gaggtccatc aggcgtaaaa cgtcccaata actgaaagag
     4981 gttacccaat cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa
     5041 ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata
     5101 acgccaatat gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc
     5161 tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc
     5221 ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattt
     5281 gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat
     5341 gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct
     5401 tttgtgatgc aagacgcaga ttatttaata aagacctggc atccctcttg tggagcagcg
     5461 gcgttacgtg ccgaattgat ggccggattt gcgcatactg aatggttggg attgaccgta
     5521 tttgaacatt gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt
     5581 accgaaggtg gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt
     5641 caatggtact atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt
     5701 ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc
     5761 aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac
     5821 tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga
     5881 gttaaatatc gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg
     5941 cacggaactg gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt
     6001 gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt
     6061 cactaaagaa gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggcctgga
     6121 tgtcgaaatc gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt
     6181 tgatattccg tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa
     6241 gatggcggaa gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg
     6301 ggtattaacg ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc
     6361 attcctgcct gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa
     6421 gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat
     6481 gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg
     6541 tgacgtcgag aaaaacgtct taagtcgtgc gctatacaaa gtactggcac agcgcgtctt
     6601 tgtttacggt aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta
     6661 atccgttacg gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc
     6721 ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc agtaaaaagc
     6781 attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat
     6841 cgacttcgaa ggttcgaatc cttcccccac caccatcact tttcaaaagt ccgtaaactc
     6901 tcaaacgaat ccgcaattaa atattctgcc caagcgggga aggatgagaa gcttcgacca
     6961 aggttcgact tgagcgccag cgagagagcg ttgccgcagg caacgacccg aagggcgaag
     7021 cgcgcagcgc tgagtaatcc ttcccccacc accatcgctt ccttaaataa tcaaaacctg
     7081 ttactccgag ctaattttgc ccgatggcac cacgtttacc gggcctacaa tatcctcccc
     7141 tgtaggtcgg ataagcgaag cgcatccgac aactcacccc gcaccgtgcc cataaaaaaa
     7201 ccccgccgaa gcagggtcta tgtgaactaa aattcgctta gtgacgagcg cgcaccagct
     7261 ggtacttacg tgtcagatac tccaccggta cgctccagat gtgtaccaga cgcgagaacg
     7321 ggaacagcag gaataacgtc atccccagca ccaggtgcag acggaagata aacgccacac
     7381 catcgaggtg ttgagaagcg ccaccgtgga aggtcaccac cgactgcgcc cagccaacca
     7441 gtttcatcat ctcgctaccg tccatatgct gagcggagaa cggaatggtc aacaggccca
     7501 gcgcgcactg gataacgagc agcgacagga tcaggatatc cgctccggta gtggttgcac
     7561 gcacgcgtgg gctgaacaga cgacgcttca gcaacagcac gccgccaatc agacacagca
     7621 cgccgctggc accaccagca aacatcgcca ttttctgttt cacttcaatc ggcagccagg
     7681 cttcatacat ccagtgcggc gtcagcatac cgaagaagtg accaacaaaa atccccagaa
     7741 taccgatatg gaacaggttc gacgccaggt tcatcccttt gcgatccagc atctggctgg
     7801 acgccgcgcg ccaggtgtac tgcccgtagt cataacgcag ccagctacca atcaggaaga
     7861 ccgccccggc tatgtacggg tagatatcaa agaagaacat attcaggaat tgcattagtg
     7921 ctgtcctccg gtggtgatat tcagatattg cggcgcgacg gcaccggcaa agcgacgctg
     7981 atgagcagtg attgctgaat caccgcagcc tttgtcagca aagaatttaa cctgctcttc
     8041 ttcccagaca gcatccagcg cctgcggcgt atcatcgcgc gcttcgtcgg caattttttc
     8101 cgccactttg tcgctgtcga tagcggtatt ggccaatttc agcagcagat caaacatcac
     8161 ggcataacgg ctttcacgct gttgcagacg cgcgctcagc aatgccagaa tcggcgcgat
     8221 atctttcaaa ccttccacgg cttcgctttg cggcagctgc gacaggtact ccagatacag
     8281 cggcagatgg tccggtaatt cgcggctgtt taactgcaag ccgtgctgct cgtactgcgc
     8341 cagcaggtcc accatcgcct ggccgcggtc acgggattca ccgtgaacat gttcaaatag
     8401 caacagtgac gtggcgcgac cacggtcgaa cagttcgctg tactgggcct gggcatcgag
     8461 cggatccatc gccgttaaat cgcgcaggaa aatgcccagc gcatgggcat cttctttcga
     8521 cagatttttc gacgcagcaa tcgcctcaaa catctcttgt tgatgctgcc ataaggcagc
     8581 atccggatat tcaaggagac gcgatacaat cacgagttcg atcatggatg cggctccgtt
     8641 ttgctggtca catcgatggc atcgatacga cggctgttga acagattgaa tttggtatct
     8701 gaaccgtggc agccatcacc aaaggtaaag ccgcagccat ttttctccgg gaaggcttcc
     8761 cgtgccagtt cacgatgact actcggcacc acaaagcgat cttcgtagtt agcaatcgcc
     8821 agataacggt acatctcctg tgcctgggct tcggtcagac cgacctcttc cagcgcacgg
     8881 gtatcaactt taccgtcaac ggtttcagca cgtttgtaat gacgcatcgc cagcatacgt
     8941 ttcagtgcgc gcagtaccgg tttggtatca ccggcggtca gcagattcgc cagatactgt
     9001 accggaatac gcaagctttc gacgtctggc agaatgccgt tgctacccag ctcacccgcg
     9061 tctgctgcag actgaatcgg agacagaggc ggcacgtacc agaccatcgg cagtgtgcga
     9121 tattctggat gcagcggcag cgccagtttc cattccattg ccattttata aaccggcgac
     9181 tgctgtgcgg cttcaataac gctcagcgga ataccgtctt taatcgcctg ctcgatgact
     9241 ttcggatcgt tcggatcgag gaacacctcc agctgacgct ggtaaagatc tttctcgttc
     9301 tcggtgctgg ctgcacgttc aatcgcgtcg gcatcgtaca gcagcacgcc aagataacgg
     9361 atacgaccga cacaggtttc tgagcacacg gtcggctgac ccgcttcaat acgcggatag
     9421 cagaagatgc acttctcaga cttaccgctc ttccagttga agtagatttt tttgtacggg
     9481 catccggtga tgcacatacg ccagccacgg catttatcct ggtcgatcag gacgatgcca
     9541 tcttcttcac gcttgtaaat cgcaccgctc gggcaggtcg ccacacatgc cgggttcagg
     9601 cagtgttcgc acaggcgcgg caaatacatc ataaaggtgt tttcgaactg gctatacatc
     9661 gccttctgga tgttgtcgaa gttcttgtct ttcgccagtt tgtcaaactc accacccagg
     9721 tcatcttccc agttcggccc tttttcgatt ttcgccatcc gttccccggt aatcagcgaa
     9781 cgcggacggg caatcggctg cgatttgctg ccttccggcg cggtatgcag gttctgatag
     9841 tcaaaatcga acggctcgta ataatcgtcg atccccggca gatgcgggtt agcgaagatt
     9901 ttacccagca acatggcacg gttacccatg cgcggctgca gtttgccgtt gattttacgg
     9961 atccagccgc ctttgtattt ttcctggttt tcccagtcag tcgggaagcc ctgacccggc
    10021 ttggtttcca cgttgttgaa ccacgcgtat tccacgcctt cacggctggt ccagacgttt
    10081 ttacaggtaa ctgaacaggt gtggcagccg atgcacttat cgagattcag caccatgccg
    10141 acttgtgaac gaattttcat tttacgctct cctgtacctg gtcattgcct tcgccatcta
    10201 accagtcaat gttcttcatc ttacgcacta caacaaactc atcgcggtta gaacctacgg
    10261 tgccgtagta gttaaagccg tatgccagat gggcatagcc gccgatcata tgcgtcggtt
    10321 tcggcgtgat acgggtgacc gagttatgga taccaccacg ctgttgggta atttccgaac
    10381 caggcaggtt aacgatacgt tcctgcgcgt ggtacatcat ggtcatccct gccggaacac
    10441 gctggctgac aaccgcacgg gcagtcagag caccgttgct gttgaatact tcaatccagt
    10501 cgttatcggc gatacccaga tctttggcat cggcttcact caaccagacc accggaccac
    10561 cgcgacctaa agtcagcatc agcaggttgt cgctgtaggt ggagtggata ccccacttct
    10621 ggtgcggcgt caggaagttg agcgcttttt ccgggttgcc gttggatttc tggcctatca
    10681 cttctttcac cgaacgggtg tcgatcggcg gacgataaac cagcaggctt tcaccgaaat
    10741 cacgcatcca ctggtgatcc tgatacagtt gctgacgacc agagagcgta cgccatggga
    10801 tcagctcgtg aacgttggtg taaccggcgt tgtaagaaac gtgttcatct tccagaccag
    10861 accaggtcgg gctggagata attttgcgcg gctgtgcctg aatatcgagg aagcggatct
    10921 tctcgtcttc tttattcagc gccagatgcg tatggtcacg accggtaaat tcgctcaggg
    10981 cagcccaggc tttcacggct acctgaccgt tggtttccgg tgccagtgtc aggatcatct
    11041 ctgccgcatc aattgcggtg ttcagcatcg gctggccttt cgccggacct tccgctttgg
    11101 tgtagttgag cttacgcagc agatccatct cgctctgggt gttccaggca atccctttac
    11161 cgccattacc gattttctcc atcaacgggc caatagaggt aaagcgttcg taagtcgccg
    11221 gataatcgcg ctctacgacc ataatgtgtg gcgcggtttt acctgggatc aggtcgcact
    11281 cgcctttttt ccagtctttc acatccagcg gctgcgccag ttcagcggca gagtcatgct
    11341 ggataggcag cgtgacgatg tcggtttctt tacccagatg gccgacgcac acttcggaga
    11401 atttcttcgc gatggctttg tagatttccc agtcgctttt cgcttcccag gccggatcga
    11461 ccgccgcaga cagcgggtga ataaacggat gcatatccga agtattcatg tcgtcttttt
    11521 cgtaccaagt cgccgtcggc aaaatgatgt cggaatagag acaggtgctc gacagacgga
    11581 agtccagcgt aaccaccaga tccagcttgc cttccagacc attgtcctgc cagtccactt
    11641 cttccggctt cacgccgccc tgttgcccca gatctttacc ctggataccg tgctccgtcc
    11701 ccagcaggta cttgagcata aactcatgac ctttaccgga agaaccgagc aggttagaac
    11761 gccagatgaa caggttgcgc gggtggtttt taccgttttc tggttgttct gccgcaaaac
    11821 ggatggaacc ctctttcagg gatttcaccg tatagtccac cggattcatc ccggcttttt
    11881 ccgcttcgcc agcgatagtc agcgggttag tgcctaactg cggtgcagac ggcagccagc
    11941 ccatgcgttc cgcacggacg ttaaagtcga tcaagtgtcc ggtatagcgg gatttgtccg
    12001 ccatcggtga cagcaactct tccgccgtga cggtttcata acgccactgg ctggagtggt
    12061 tatagaaata agaagtgctg ttcatgtgac gcgccggacg ctgccagtca agggcaaacg
    12121 ccagcggctg ccagccggtt tgcggacgca gtttttcctg acctacatag tgcgcccagc
    12181 cgcccccgct ctgaccgaca cagccgcaga aaatcagcat gttgatcaga ccacgatagt
    12241 tcatatcgag gtgataccag tggttcagcc ccgcaccgac gataatcatc gaacgaccgt
    12301 gcgttttatc agcgttatcg gcaaattcac gggcgatgcg aataatctgg ctgcgagaaa
    12361 cgccggtaat ctgctcggcc caggccgggg tataagcttt cacatcgtca tagctggttg
    12421 cacagttaac gtcgttcagg ccacgttcca gaccgtagtt tgccagcgtc agatcataaa
    12481 cggtgctcac cagggcggtg ctgccatcag ccagttgcag gcgtttcacc ggcagtttgt
    12541 gcagcagcac gttttccagt tccactttgt tgaagtgttc cgtgccgtcg ccaccaaagt
    12601 acgggaagcc tacctcagcg atctcatcct ggctacccag caggctcagt tgcagctcgg
    12661 tttcttcgcc agttttgccg tcgcgctgct caagattcca tttgcccttc tcgccccagc
    12721 ggaagccaat agaaccgttc ggcgcaacca tttcgccatt ggtattaaag gcgacagttt
    12781 tccattccgg attgttttcc tggcccagcg catcaaccag atcagcagcg cgcagcatac
    12841 gacctgcagc gtagtaaccg tcgcgttctt ccagcatcac cagcatcggc atgtcggtgt
    12901 agcgacgcac atagtcggtg aaatactggc ttgggttgtc gaggtggaat tcacgcagca
    12961 ttacgtggcc catcgccagc gccattgccg catcggtgcc ctgtttcggt gccagccaca
    13021 gatcgcacag tttggcgatt tcagcgtagt ctggtgtgac ggcaacagtt ttggtgcctt
    13081 tgtaacgcac ttcagtaaag aagtgagcat ccggggtacg cgtctgcggc acgtttgaac
    13141 cccaggcgat gatgtagctg gagttatacc agtcagcaga ttccggtacg tcagtttgct
    13201 cgccccaggt ttgcggagac gcaggaggca ggtcgcagta ccagtcgtag aagcttaagc
    13261 aagtaccgcc aatcagcgag agatagcgtg cacccgatgc gtaagaaacc atcgacattg
    13321 ccggaattgg cgagaaacca gcaacacggt ccgggccgta gtttttgatg gtgtaaacgt
    13381 tagatgcggc gatcagttcg ttcacctcct gccaggaaga acgaacaaat ccaccgcgtc
    13441 cacgcgcctg cttaaagctt ttcgctttat cggcgtcttc aatgatagaa gcccatgcct
    13501 caaccggatc gctatgcagc gccttcgctt cacgccacat tttcatcagg cgtttgcgca
    13561 tcatcgggta tttcaggcgg ttggcactgt aaagatacca ggagtagctg gcaccgcgcg
    13621 ggcagccgcg aggttcatgg tttggcagat ccggacgggt acgcggatag tcagtctgct
    13681 gggtttccca ggtgaccaga ccgtttttga cgtagatttt ccagctgcag gagccggtgc
    13741 agtttacccc gtgggtagag cggacgattt tgtcatgctg ccaacgctgg cgatatccat
    13801 cctcccagtc acggttggta ttgagaagct ggccatgccc atcggcaaag gtttcaccct
    13861 tctgtttgaa gtagcgaaac cggtccagga atttactcat cggttttctc ctgtgggagc
    13921 ctgtcggccc tctgataaat cgacattgct aatgtttaag ggcaaggtaa cgctctgaaa
    13981 cagcgtggga attgataacg atcaagagtg atggggatga aaaataaagt aaattcctaa
    14041 gatacccctt taggataatt aacagccacc acataacaga ctgtaaatta agacgtttta
    14101 tacctgtagt tatttaaact attaagattt ttatgcagat gggtattaag gagtattccc
    14161 catgggtaac atattaatgg ctcttaatga aatccggcga aataagccat ataatatgag
    14221 tatgttgtaa ctaaatgacg cataaaaaaa gcgcgacatc atgccgcgca aaggataatc
    14281 aaatattaag atttattttt tagaatgccg accatatacc gcccaggtaa tcactacgca
    14341 ggcgatatag aaaatcaaaa ataccttcat tgcgccgact ggcgaacccg ttaatgccag
    14401 cgagctacca aacgctttcg ggataaagaa gccaccaata gcgccaatcg cagagatgaa
    14461 acccagcgcc gctgccgtgt cggttgccgc ttcacgcatc gcacgttcgt cagaaccccc
    14521 ttctgctttc acgcgatcca ttgtcagttt acggaagatc actgaaatca tctggaaagt
    14581 ggaaccacta cccagcccag ctgtcaggaa cagcgccagg aagaccgcga agaacgccat
    14641 gaagcttccg ccctgcccgt cagtcggtaa ggtcaggaac agcaggccgc tgaaaatcgc
    14701 catcagaata aagttcacca gcgtgacacg agttccgccc agacggtcag ataatgcacc
    14761 acctgcagaa cgcgccagcg caccaataaa cggcccgaag aaagcgtatt gcagaatctg
    14821 aacatccggg aactgcgttt ttgacagcat cgcaaagccc gcggagaagc cgataaagga
    14881 gccgaaggtt gccagataca gcaggctcat aatccacaga tgaccccgtt tgagtaccgg
    14941 caactgctcc ttgatggagg ctttcgaggt agcaagatcg ttcatgccaa accacgccgc
    15001 aatggtgaag atggcaagga acggcaccca tatccaggac gcattcgcca gatacagctc
    15061 agtcccatcc ggctgtttga cgccctggct accaaatact gcgaaaatcg acagtgatac
    15121 caccagcgga gcaaccaact gcatgacgct gacgcccatg tttcccagac caccattcag
    15181 acccagcgcg ccaccctgct tctgtttcgg aaagaagaag ctgatgtttg ccatactgga
    15241 tgcgaagttc gcgccagcaa agccgcatag cagagagatg atgatgaaga cgctataagg
    15301 cgtggaggta tcctgcacgg caaaacccag ccagacgcaa ggaataatca gaataccggt
    15361 gctgaacgcc gtccagcgac gaccaccgaa gataggaacc ataaaggagt atggaacacg
    15421 taataacgcg ccagaaaccg aaggcagcgc agtcaacata aatagctgat cggtcgtaaa
    15481 attaaaaccg actttcggta agttcaccgc aacagcgctg aacaacatcc atacgcaaaa
    15541 cgccagcagc agacagggaa cggaaatcca caggttacgg ctggcaatac gttgaccgcg
    15601 ttgttgccag aacgcaggat cttccggtcg ccaatctgta atgacagctc cagtagccct
    15661 ttcgggggcg gatgagtgac tcatagacac ctctgatact cgtttcgatg tctgccacct
    15721 tagtgtctgt agctaaaggc aatttgatgt aaatcaaacg ataagataac tttatctatc
    15781 attgatattt atcattaccc atagtgagta cagtgatttc ataaaaatta tgagattttt
    15841 cacggtgctg taaaatccct acccttaccg atgtaaagcg actaaccaca cggcaaataa
    15901 ggagtaactc tttccgggta tgggtatact tcagccaata gccgagaata ctgccattcc
    15961 agaatgtatc gtcacattca ttaaggttat tgctcattta aagcctgaag gaagaggttt
    16021 gcatgcttaa acgttgtctc tctccgctca ccctggttaa tcaggttgcg cttattgtgt
    16081 tgctttctac tgctattgga ctggcaggga tggcggtttc tggctggctg gtgcaaggcg
    16141 ttcagggcag cgcccatgcg atcaacaaag cgggatcgct gcgcatgcaa agttaccgtc
    16201 tgttggcggc agtgccatta agtgagaaag acaagccctt aattaaagag atggaacaaa
    16261 cggcatttag cgccgagtta actcgagcag cagaacgaga cgaacaactg gcgcaattac
    16321 agggtttaca agattactgg cgtaatgaac tgatccctgc actgatgcgt gcacaaaacc
    16381 gcgaaacggt gtcagcggat gtcagccagt ttgttgccgg gcttgatcag ctggtatctg
    16441 gttttgaccg caccacggaa atgcgcattg agacagtggt actggtccat cgggtaatgg
    16501 cggtatttat ggcactttta ctggtgttca ctattatctg gttgcgggcg cgactgctac
    16561 aaccgtggcg gcaactgctg gcaatggcga gtgccgtcag tcatcgcgat tttacccaac
    16621 gcgcgaacat cagcgggcgc aacgaaatgg cgatgcttgg aactgcattg aacaatatgt
    16681 ctgcagaact ggccgaaagt tatgccgtac ttgagcagcg ggttcaggag aaaaccgccg
    16741 ggctggagca taaaaatcag atcctctctt ttttatggca ggctaaccgc cgtttgcatt
    16801 cccgcgcccc gctgtgtgaa cgcctgtcac ctgtactcaa cggcttacag aatttaaccc
    16861 tgctacgtga tatcgaattg cgggtgtatg acactgatga tgaagagaat catcaggagt
    16921 ttacctgcca gccagatatg acttgtgatg ataaaggctg ccagctctgc ccgcgcggcg
    16981 tattacccgt tggtgatcgc ggcacgaccc tgaagtggcg gctggctgac tctcatacgc
    17041 agtacggtat tttgctggcg accctgccgc aggggcgtca tcttagccat gatcaacaac
    17101 aactgctgga taccctggtt gaacaactca ccgccacgct ggcgctggat cgccatcagg
    17161 aacgtcagca acagttgatc gtgatggaag agcgtgccac cattgcgcgc gaactgcatg
    17221 attctattgc ccaatctctc tcttgcatga agatgcaggt gagttgttta cagatgcagg
    17281 gcgatgcgct gccagaaagc agccgcgaac tgttaagtca gatccgtaac gaactgaatg
    17341 catcctgggc gcagttgcgt gaattgctca ccacattccg cttgcagctc accgagcctg
    17401 gattacgtcc ggcgctggag gcgagttgcg aagagtacag cgccaaattt ggcttcccgg
    17461 tgaagctgga ttatcaattg ccgcctcgcc tggtgccttc gcatcaggca atccacttgt
    17521 tgcaaattgc ccgtgaggca ttaagtaacg ccctcaaaca ttcgcaagcg agtgaagtcg
    17581 tggtgacggt ggcgcaaaac gataatcagg tcaaactgac cgtccaggat aacggctgcg
    17641 gcgtgcctga aaatgccatc cgcagcaatc actacggcat gataataatg cgcgaccgtg
    17701 cgcaaagttt acgaggcgat tgccgcgtcc gccgtcgtga atcaggtggc accgaagtgg
    17761 tggtcacctt tattcccgaa aaaactttca cagacgtcca aggagatacc catgagtaat
    17821 caggaaccgg ctactatcct gctgattgac gatcacccga tgctgcgaac tggcgtaaaa
    17881 cagcttatca gtatggcacc agatatcacc gtggttggcg aagcgagtaa tggcgaacag
    17941 ggtattgaac tggcggaatc tcttgatccc gatctgatcc tgttagatct caatatgccc
    18001 ggcatgaacg gtctggaaac gctggataaa ctgcgcgaaa agtccctctc agggcgcatt
    18061 gtggtattca gcgtctctaa ccatgaagaa gatgtggtca ccgcactgaa acgcggcgcg
    18121 gatggttatc tgttaaaaga tatggaaccg gaagatctgc tgaaagcatt gcatcaggca
    18181 gctgctggcg aaatggtatt aagcgaagca ttaacgcctg ttctggccgc cagcttgcgc
    18241 gctaaccgtg ccactactga gcgcgatgtt aaccagttaa ccccacgcga gcgcgatatt
    18301 ctcaagctga ttgcccaggg tttgccgaac aagatgattg cccgccgcct ggatatcacc
    18361 gaaagcacag taaaagtgca cgtcaagcac atgctgaaga aaatgaagct caagtctcgc
    18421 gtggaagcag cggtatgggt gcatcaggag cgcattttct gattacggtt cccagcgcag
    18481 ttcgtcgttt gacaatgcat cgaacggttc gacaagcgtt agcgtgatct cattggagga
    18541 gacacgctgc ccctggttat cttccaccac caccgacaat cgccagtgat tgctcgcccc
    18601 ttccccgttc tgccagtcag gcatgatcag cgtccagccc tccgcgctgt tggcttgtgc
    18661 gcccggcgtc aaacttaata tctgcgtatc gccctgccaa atcagttgcc gaataccgta
    18721 acgactgcgg atttgtaatt tcagcggcac tgtttcgcca ggttttagat cccacggcgg
    18781 tgtcgccaga aacaccgtta acgtttttcg ctgtcggtac tcaagagtcg gtagattatt
    18841 tcgctgcgga ttatcatagc gactaccacg taacgactga ctttcggcaa cctcgcccgc
    18901 agaaagttgt tttttgagcg gcacaccaaa gcggtaatta agattcagtc cgaggttatt
    18961 ttgattttcg ccactttcac cctgtttatg ctgggccgtc acagtgacta atggcacagg
    19021 ggtgtaattt aatcccagac tcaacgcgac gggattgtga taacccgtac cagagttaaa
    19081 caaatcaaca cgctcaccaa aatactgttc taccctgaca ctggtattga ggtgttgata
    19141 gaacggcatg cgcatccggg ctgtcaggtc atacccgcgc gccatccgtt gttcctgcgt
    19201 ggctgtctgt tcatgccatg cagcaaacgg ctgataaaag tttgccgata gtcgcaaata
    19261 ttcgccccac gcttcggcac caaagcccgc tcgctgaaga ttttcgtcca gcaagttgtc
    19321 ataaaaagtg ttataaccca ccagccagtt gccgcgcgcc cagcgttgcc caacgcccac
    19381 attgctcacc aacccatcat cctgctgagt aagaccaagc tggctccagg tgagataacg
    19441 atcattatct tgtaacggca caaaccagct tccacgactg ccggtgaaat gtccttcgtt
    19501 atccactttg acgtcaacac tggcatttcc ccacggtgat agccaggact ctacgtgctg
    19561 attaacctgt tgactaagcg cgtcgcggac ttttcccaat gcgaaagctt ttgcctgctc
    19621 gccagtatcc agcccgttat cattcatact ggtttcgccg aaatctttca caatttcagc
    19681 aaagtgtttt tccccatcat ggttttcggg agccatgcct aaatccggca gaccatcatt
    19741 gttattatca aagggatttg ccgctttttg ctcgaaggta gattgtgcgt tagctgtacc
    19801 gcctgccgcc agcaagagta aataaaacgg gataatgcga ggaacgaaac ggctcaaaac
    19861 gtcggtaact tccggtaata atgaaaaacc atagccttaa agaataacga ttactggcaa
    19921 cttttgtcgt attttctcgc gctttcagga aaatccttaa aaaggtgacc gattggtcag
    19981 acgctgcaga agcagtaaga tagaagatga gtgtctaatt ccctgaatac aggagtaaat
    20041 aatgcaaaaa atcgtgatcg ttgccaatgg cgcaccttac gggagcgaat ccttgtttaa
    20101 cagcttgcgg ctggccattg cgttacgaga gcaggagagc aatctggatc tgcgtctgtt
    20161 cctgatgtct gatgcggtca cagccgggtt gcgcgggcaa aaaccagggg aaggctacaa
    20221 cattcagcaa atgctggaga tccttaccgc tcagaatgta ccggtgaaat tgtgcaaaac
    20281 ctgtaccgac gggcgcggga ttagcacact tcctcttatt gatggggtgg aaatcggtac
    20341 tcttgtggaa ctggcgcaat ggacgctgtc agccgataaa gtgctcacat tttaatgttt
    20401 gagggggcgt ctgacgcccc actatgaggg aagtttactc aggcgaatcc cggccgtaac
    20461 acaccatccg gataattctc cgccagcagt ttttttaccg ataccagcaa atcattcagc
    20521 ccatcgtcct gcattcccag tttgataagc tcctgttcca gtgaaaacag gtattgtgcg
    20581 ttggttccca gcggaccgct cgccgccgca atcaacggcg cgatgacctg agcgcgagtg
    20641 tcagattcat attctggatg tcgcgggtcc ataataaaca caatggcgtt tactgtgcgt
    20701 ccatcatcaa gatcaagctg acaccaggtt ggcagataac agccggtaat catctcgcgc
    20761 ttccacaaca gggttagttc ctgctccagc gtctcttctg gtagtcgata ggcgacgcct
    20821 gtggtgcgtc cgccctcttt cagtgcaagc atccgtcccg gctggtgcgc agtcccgcgc
    20881 ccggcggtca ggcgcaggca aaacgcgcga tgccatccaa ccagtgtacc ggtgcataat
    20941 tcggtaaact ccagtgccgg attccacatc aacgatccat aaccgaagat ccacaccggt
    21001 ccctcattag gtcgacaagc cagcgtcgcc gccagcgaag ccgcccgttg ttctgctgac
    21061 cataagagtg attcttcaat ggcaccaaat gccgttttac aatcggcatt catcaagaaa
    21121 tcacgcgtta tcaccttaca cctccgccac tgcatacttc cctgcgacaa ctttttggtg
    21181 tcttccggac atttttaccg ctcattttga aaccactaac gggacaatat gcaatccacg
    21241 gaccacttgc aaggcaacac cgcacgaata gctttaacta agctaccggt tttacgattt
    21301 tttatgccat ttatcatcat cccctttggc atattcatgc ttcacagcag cccaggccac
    21361 tttatgcgcg gtttcttcgc gactggcgtc atcacgccga tcttctttat ctttatattg
    21421 atcccatgcg ctgttgaacg cttctttata gatatcctgg gcatgagacg gtagaacgtg
    21481 ctttacgctt tccggcagat cgcttttcgt tttatacggc atcgttgact ccttttacaa
    21541 agttgaacat taagtgtggt caacaatatc tactcttgcc aaaacataag aattttcatg
    21601 tcagactgtt cttaagaaac gctaaaacaa acattgtcta aacgtaatac ttatttttat
    21661 gccccaatga gtaaaaaatc gacagcgggg taaaaattcg taaaatgtta ccgatatcca
    21721 gacactttct gttatttttc ctacctattt tttacatttt cgataaattc atctgcaaag
    21781 ggataaccat aatgtcaaat gctcaagagg cggtaaaaac ccgccacaag gagacttcgc
    21841 ttattttccc ggttctggcg ctggtagtgc tgttcctgtg gggaagcagc cagacactac
    21901 cagtggtcat tgccatcaat cttcttgcgc ttattggtat tttaagtagc gcctttagtg
    21961 ttgtccgtca tgcggacgta ttagcccatc gcctgggaga accttacggt tcgcttattc
    22021 ttagcctttc agtggttatt cttgaagtca gtttgatttc agctttaatg gcaaccggcg
    22081 acgccgcgcc aacgctaatg cgtgatacgc tctattcaat cattatgatt gttaccggtg
    22141 ggctggttgg cttttcatta ttgttgggcg gtcgtaagtt tgccacccaa tatatgaatc
    22201 tgtttggtat caagcagtat ttaattgcgc tgttccccct ggcgataatc gtactggtat
    22261 ttccaatggc tctgcctgcg gcgaattttt caaccggtca ggcgttactg gtagcattaa
    22321 tttctgcggc aatgtatggc gtatttttgc tgatccagac caaaacgcat caaagtttgt
    22381 ttgtctacga gcacgaagat gacagtgatg acgacgaccc gcaccacggt aaaccgtctg
    22441 cccatagcag cgtgtggcat gctatctggt tgattatcca tctgattgcc gttattgcag
    22501 tcaccaaaat gaatgccagc ccgctggaga cattgctcga cagcatgaat gcccctgtcg
    22561 cctttactgg cttcctggtg gctctgttga ttctgtcgcc ggaaggttta ggtgcgttaa
    22621 aagcagtgtt gaacaaccag gtccagcgcg caatgaatct gttctttggt tcagtgttag
    22681 caaccatttc gcttaccgta cctgtcgtca cgctaattgc ctttatgacg ggtaacgaat
    22741 tacagtttgc acttggtgcg ccagaaatgg tggtgatggt ggcctcttta gtgctgtgcc
    22801 atatctcctt ctccaccgga cgtactaacg tgctcaatgg cgcagcgcat ctggcactgt
    22861 ttgccgccta tttgatgacg atatttgcct gacagttata tctcctccga tgacggaaat
    22921 cgggggagat aagataaatt cctgcttatt gtatttcttc atactacctg gacagctcgt
    22981 taatcagaat tgcgagcctg taccacaacc tgcacgaaac cactgcaaca gctgttgaaa
    23041 agccggaatg tactctttat tgcaaacatt tcagggatga agtgaagatg aattgagacg
    23101 tagtatccac accaagccgg aattatccgg tgaggcgcaa tgttgcgggg gctttatccc
    23161 tggtggcatt ggttgctgga aagagaaaac ccccgcacgt tgagaggtaa aacctgacaa
    23221 caaaacgggg gctaatcttg aaaccagaca cttcaagaat agccgccttg ttgtcattac
    23281 aacaaggcgg ctatatgacg ctcgcgcagt ttgccatgac tttctggcac gacctggcag
    23341 caccgatcct ggcggggatt attaccgcag cgattgtcag ctggtggcgt aaccggaagt
    23401 aaggtgaaaa cgggcgtcag gctgccgtga tggcaatatg cgcccgaaac cggccgcagg
    23461 ggtaacagcc tgcggccatt tttgtcttta tctcttccac aagtaaatat tggcaacccg
    23521 agctggaagc atcatccaga gcactcatga aacgactgta acagctgttg aaaaaccgga
    23581 atgcactctt cattgcaaac atttcaggga tgaagtgaag atgaattgag tcgtagtatc
    23641 cacaccaagc cggcgttatc cggtgaggcg caatgttgcg ggggcttaat ccctggtggc
    23701 attggttgct ggaaagagaa aacccccgca cgttgagagg taaaacctga caacagaacg
    23761 ggggctaatc ttgaaccatc aacaattcaa gaatagccgc aaaatgttgt cattacaaca
    23821 aggcggctat atgacgctcg cgaagtttgc catgattttc tggcacgacc tggcagcacc
    23881 gatcctggcg gggattatta ccgcagcgat tgtcggctgg tggcgtaacc ggaagtaagg
    23941 tgaaaacggg cgtcaggctg ccgtgatggc aatatgcgcc cgaaaccggc cgcaggggta
    24001 acagcctgcg gccatttttg tctttatctc ctccacaagt aaatattggc aacccgagct
    24061 ggaagcatca tccagagcac tcatgaaacg actgtaacag ctgttgaaaa accggaatgc
    24121 actcttcatt gcaaacattt cagggatgaa gtgaagatga attgagacgt agtatccaca
    24181 tcaagccggc gttatccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg
    24241 gttgctggaa agagaaaacc cccgcacgtt gagaggttta acctgacaac agaacggggg
    24301 ctaatcttga accagaacaa ttcaagaata gccgcaaaat gttgtcatta caacaaggcg
    24361 gctatatgac gctcgcgcag tttgccatga ttttctggca cgacctggca gcaccgatcc
    24421 tggcgggaat tattaccgca gcgattgtcg gctggtggcg taaccggaag taaggtgaaa
    24481 acgggcgtca ggctgccgtg atggcaatat gcgcccgaaa ccggccgcag gggtaacagc
    24541 ctgcggcctt ttttgtatct ggcacccatg aaaaaagtct taacgcagaa cgctaaggct
    24601 ttatttttca agcaaaaaag attacttgct ggtatccagt tcttcgaaac ctttcaccag
    24661 atcatcaatc gctttcatct gcttgaggaa cggttccagt ttagccagcg gcagcgcgga
    24721 tggaccatca catttcgcat gttccggatc cggatgcgct tcaataaaca gccccgccag
    24781 acctaccgcc ataccggctc gtgccagctc agccacctga gcacgacgac caccggaagc
    24841 tgcgccaaac ggatcgcggc attgcagtgc gtgggtcacg tcgaaaatca ccggcgagtt
    24901 accagacact ttcttcataa tgctgaagcc cagcatatcg acaaccaggt tgtcatagcc
    24961 gaagttagca ccgcgatcgc aaagaatcac tttttcgttg ccgccttctt tgaatttatc
    25021 aacgatatta cccatctgtc ccgggctgac aaactgtggt ttcttgacgt taattaccgc
    25081 accggttttc gccatggctt caaccaggtc agtctggcga gcaagaaacg ccggcaactg
    25141 aatcacatcc acgacatcag caacgggctg tgcctgactt ggttcgtgaa cgtcggtgat
    25201 aattttcacg ccaaaagtct gcttcaactc ctggaagatt ttcatccctt cttccaggcc
    25261 cggtccacga taagagtgga tggaggagcg gttggctttg tcaaaagagg ctttgaacac
    25321 gtaagggata cccagtttct gggtcacggt tacgtagtgc tcgcaaatgc gcatcgccag
    25381 atcgcgagat tccagcacgt tcataccacc aaacagtacg aacggcaggt catttgctac
    25441 gttgatgtcg ccaatgctaa ccactttttg tttcatagga tcgccttaat cttgagtaaa
    25501 atgtcgatta attaatgcag cacaatatgt ttatgcgcga tgttatttat ttgcgcacgg
    25561 atcatttcgc tgatcgggtc ttccggacac tgttcaacga aataacttaa atcgttcaac
    25621 gcaacgtgtt cgcaatccag ttgcgcataa atcaacccgc gatcgcgaat ttcataggga
    25681 tcttccgggt tgaattgtaa taaagcttcg ctggtgcgta acgccagctc catctgattt
    25741 tcttccatca acgaggcttt gagtgtatcc aacaatttgc ggattacctc aatgttatca
    25801 gcttcatcaa ggtcttcata aaacagttcc gccgacgggc tgatatttcc ctttaaccat
    25861 acgtccagca tatgttcgct taacgattca ccgttaaaag gattaatcag ccaaatttcg
    25921 ccatccggac attcaatgcg caatatcagc tgcgtaggga aaatcaccgg cagcagcggc
    25981 aaatcgagac gattcgcgac ccataataaa accgcaccta atgataccgc actgcctgtc
    26041 gattctttaa cacctggtcc agccataatg catcggaaag acgataaacg ccgcgtgagg
    26101 ctttaaatcc ccagtcgccg taaaacagcg cgatcagttt ttccagctgc tcttctaaag
    26161 gcagaagctg gctgatttct tccttcgcca gactaacgag acgctccagt tcgtcgtaaa
    26221 catcttgcga gggaaaatcg cggcggattg cttcgcaagc caggatcatg ccttcgcaca
    26281 atggcgcttt attaaattcg aaatcagcta acgatctcat gacttacccc agtaacggta
    26341 cttttgtggt ggcgagttta atgatgatgt acagcaccac caacgccagc gggaaggcaa
    26401 taatgcgcgc ctgctgactg tgcatacgac gataatcgag tgcaataaaa cccaaaacga
    26461 tataaatgat aactccaaac agcttttcag tcagccactg tgcctgcccg gaaaatggca
    26521 ggatgtgcgc tttagccatc aacgcaatgc cgcttaacaa taacaccgta tcgacaaccg
    26581 gcggcacaat gcgcgtccag cgagcaaatg cctgtggatg cttctgataa cgtagccaga
    26641 aacgtaaggt taatagccca acagaaagcg cgatactaat aagatgaaca ctaagcagtg
    26701 tagaaaaact tgtcattgat aatagcggcc gagcgttacg cgctcgttat caccatagtc
    26761 acggcaggtt tcgacgtcat gataccccgc gaggataaat gcttgtcgca ccgcttcgcc
    26821 ctgctgccag ccatgttcca gaagcagaaa gccgccggat accagcgcgt tacgcgactg
    26881 ttcgatgata tgcacgatgt ctgccattcc actgtctgcc gcaaccagcg cagtgagcgg
    26941 ctcaaagcgg acatcgcctt gttgaagatg tgggtcctgc tcgtcaatat acggcggatt
    27001 gctgacaatc atcgcaaact gctgcccggc tagcgcgcta aaccagtcgc tttgcagaat
    27061 gtggatattt ttgatcgcca gatgctgggc attacgttgt gccagggaga cagcatcagg
    27121 catacgatct acagcgataa tttcgcagtc cgggcgctcg ctagccagcg caagcgcaat
    27181 cgccccggta cccgtcccga gatcgagaat acggcaaggt tgttcaggca accgcgccag
    27241 tgcctgctcc accagacact ccgtatccgg gcgcggaatt aaggtcgctg gcgaaacaaa
    27301 taacggcaac gaccagaatt ctcgcacccc ggttaaatga gcaatgggtt caccatcgcg
    27361 acgacgtgtc agtagcgcat caagttgctg acattgttcg tcagtcagct gcgtttcacc
    27421 aaaggccaga ataaaagtcc gccccctgcc agtgacatac tccaataaga tttcagcgtc
    27481 acggcgtggg ctttcgctcg cctgaagttg gcttattgct tcacgtaacc agtattgata
    27541 ttccattatt cctgctcgga caacgccgcc agttggtcgg cctgatgttc ctggataatc
    27601 ggttcaatca gcatatccag cttaccttcc atcacttcat ccagacggta gagcgtcagg
    27661 ttgatgcggt gatcggtaac gcgcccctgc gggaagttgt aagtacggtt acggtcgctg
    27721 cgatcgccac tccccagcag gttacgacgg gtagacgctt cggcctgttg ccgttttgcc
    27781 atttctgcag cgtggatacg agcaccaagt acagaaagtg ctttagcttt gtttttatgt
    27841 tgtgaacgtt cgtcctgaca ttcaacaaca atcccggtcg gcaagtgagt aatacgaatt
    27901 gccgaatcgg tggtgttaac gtgctgacca cccgcccctg acgagcggaa agtatcaatg
    27961 cgtaaatctg ctgggttgat gtccggcagt tctgcgtcag gcagttctgg cattaccgca
    28021 acggtacaag cagaagtatg aatacgaccc tgcgattccg tagcaggaac acgctgcacg
    28081 cgatgaccgc cggattcaaa tttcagacga ccatacacac catcaccgct aattttggcg
    28141 atgatctctt tataaccacc atgttcaccc tcgctggcgc tcatgatttc tacccgccag
    28201 cggcgggctt cggcataacg gctgtacata cggaacagat cgcccgcgaa cagcgccgct
    28261 tcgtcgccgc cggttccggc tcggacttcg aggaaggcgt tacgttcgtc atcaggatct
    28321 tttggcagta acagaacctg taattgctgt tccagttgct cgcttttttc tttagcttcg
    28381 cgcagttcat cctgcgccat ctcacgcatt tcaggatcat cgagcatcat ctgtgcggtt
    28441 tcgatatctt cctgaacctg ttgccagtcg gtaaaacagc gcgaaacatc acttaactgc
    28501 gcatattcgc gtgataatgc gcgaaaacgt tcctggtcgg cgatagtttg cgcgtcaccc
    28561 agcaacgcct gaacttcttc atggcgttca tgcagggctt ccagtttggc aacgatagaa
    28621 ggcttcatag gcgtaaatgc accctgtaaa aaaagaaaat gatgtactgc tactccagcc
    28681 cgaggctgtc gcgcagaata ttcaggcgtt cgttatcccc gtcacgggcg gcctgttgaa
    28741 gtgatttcgt tggcgcatgg atcaagcggt tagtcagttt ccatgccaga tcctgcataa
    28801 tggcttgcgc gtcgccgccc tgctcaaggg ccgctaacgc tttggcggtt aactcatcgc
    28861 gaacgtgctc tgcctggctg cgatactcgc gaatggtttc gctggcgctt tgtgctcgca
    28921 gccacgccat aaattcgctg gtttcctgag cgacaatagt ttccgcctca accgctgcgg
    28981 ctttacgctg cgccaggttg tgcgaaatga tgctttgcag atcgtccaca ctataaagat
    29041 aagcattcgc cagtttgcca acttccggct caacatcgcg cggaacggca atatccacca
    29101 acagcattgg ttggttacga cgacttttta atgcgcgctc caccatgcct ttcccgataa
    29161 tcggtaacgg gctggcggtg gaactgatga tgatatcggc ttcgcgcaga cgttcgtcga
    29221 tctcactcag ggcaatcact tccgcgccaa cttcatctgc cagaatttgg gcacgttcgc
    29281 gagtgcggtt ggcgataatc atcttctgta ctttatgttc gcgcagatga cgcgctacca
    29341 gctcgatggt ttcgcccgcg cctaccagca acactgtgac cgtagagagc gattcaaaga
    29401 tctgccgcgc cagcgtacaa gccgcaaaag cgacagacac agcactggca ccgatatctg
    29461 tttcagtgcg aacgcgtttc gctacagaga aagatttctg gaacatgcgt tccagttcgc
    29521 tggccttcat atgacctttt tgcgaatcgg caaacgcttt tttaacctga ccgaggatct
    29581 gcggctcccc aagaaccagt gaatccaggc cgctggcaac acgcattaaa tggctaaccg
    29641 cgtcgttatc ctgatgccag tagaggcttt tacgcagatc ttcttcatta agattgtgat
    29701 aatcgcaaag ccagcggatt aacgcctctt gcaggttatc ctgctcttca acgctaagat
    29761 aaagttccgt gcggttgcac gtcgacagca ccacgccgcc ctgcaccatc ggctgcgcaa
    29821 gcaggctgtc aagcgcctga tcgagcttat ccggcgaaaa cgatacacgt tctcgcagcg
    29881 atacaggtgc cgttttatgg ttgataccga gtgctaaaag ggtcatgtct gcgggaaata
    29941 ataccaacgt tgatatggtt agtctgcttg catcatacag gatgcgtaag atcaataaaa
    30001 gagagcgccc ccttttggag taattgccga aagccgttag attctggcaa ttaagacaac
    30061 ttgaacatag acgatagcgg acggtaacgc tagcattaag ggttataact gcaacgtatc
    30121 tcaaggactt gtcatcacta tgccccagcc cgattttcgt cttatccgcc tgctaccgct
    30181 ggctgctctt gtgctcactg cctgttccgt taccacgccc aaaggtcctg gcaaaagccc
    30241 ggattcgcca caatggcgtc agcatcagca agacgtgcgc aatcttaatc agtatcagac
    30301 tcgcggcgcg ttcgcttata tttctgacca acaaaaagtg tacgcccgct ttttctggca
    30361 gcaaaccggc caggatcgct accgtctgct gctcactaac ccattgggca gcacggaact
    30421 ggagctgaat gctcaaccgg gtaacgtgca gttagtcgac aataaaggtc agcgttatac
    30481 cgccgatgac gccgaagaga tgattggcaa attgaccgga atgccaattc cgctcaacag
    30541 cttgcgccaa tggattttag gtttaccggg tgatgcaacc gactacaaac tggacgacca
    30601 gtaccgcctg agcgaaatta cctacagcca gaatggcaaa aactggaagg ttgtttatgg
    30661 tggttatgac accaaaacgc aacctgcgat gccagccaat atggaactca ccgacggtgg
    30721 tcaacgcatc aagttaaaaa tggataactg gatagtgaaa taatgcggac acagtggccc
    30781 tctccggcaa aacttaatct gtttttatac attaccggtc agcgtgcgga tggttaccac
    30841 acgctgcaaa cgctgtttca gtttcttgat tacggcgaca ccatcagcat tgagcttcgt
    30901 gacgatgggg atattcgtct gttaacgccc gttgaaggcg tggaacatga agataacctg
    30961 atcgttcgcg cagcgcggtt gttgatgaaa actgcggcag acagcgggcg tcttccgacg
    31021 ggaagcggtg cgaatatcag cattgacaag cgtttgccga tgggcggcgg tctcggcggt
    31081 ggttcatcca atgccgcgac ggtcctggtg gcattaaatc atctctggca atgcgggcta
    31141 agcatggatg agctggcgga aatggggctg acgctgggcg cagatgttcc tgtctttgtt
    31201 cgggggcatg ccgcgtttgc cgaaggcgtt ggtgaaatac taacgccggt ggatccgcca
    31261 gagaagtggt atctggtggc gcaccctggt gtaagtattc cgacaccagt gatttttaaa
    31321 gatcctgaac tcccgcgcaa tacgccaaaa aggtcaatag aaacgttgct aaaatgtgaa
    31381 ttcagcaatg attgcgaggt tatcgcaaga aaacgttttc gcgaggttga tgcggtgctt
    31441 tcctggctgt tagaatacgc cccgtcgcgc ctgactggga caggggcctg tgtctttgct
    31501 gaatttgata cagagtctga agcccgccag gtgctagagc aagccccgga atggctcaat
    31561 ggctttgtgg cgaaaggtgt taatctttcc ccattgcaca gagccatgct ttaagccggg
    31621 caagctgagt ttcggtgaca acgtcacctt gttccagacg ttgcatcgcg ctctttaata
    31681 caccgcctgg aaaggatcat gcctggcccg cacagttttc ggcagattct ttccaccaat
    31741 ggacgcatgc ctgaggttct tctcgtgcct gatatgaagc tttttgctgg taacgccacc
    31801 ccggaactag cacaacgtat tgccaaccgc ctgtacactt cactcggcga cgccgctgta
    31861 ggtcgcttta gcgacggcga agtcagtgta caaatcaacg aaaatgtacg cggtggtgat
    31921 attttcatca tccagtccac ttgtgcccct actaacgaca acctgatgga attagtcgtt
    31981 atggttgatg ccctgcgtcg tgcttccgca ggtcgtatca ccgctgttat cccctacttt
    32041 ggctatgcgc gccaggaccg tcgcgtccgt tccgctcgtg taccaatcac tgcgaaagtg
    32101 gttgcagact tcctctccag cgtcggtgtt gaccgtgtgc tgacagtgga tctgcacgct
    32161 gaacagattc agggtttctt cgacgttccg gttgataacg tatttggtag cccgatcctg
    32221 ctggaagaca tgctgcagct gaatctggat aacccaattg tggtttctcc ggacatcggc
    32281 ggcgttgtgc gtgcccgcgc tatcgctaag ctgctgaacg ataccgatat ggcaatcatc
    32341 gacaaacgtc gtccgcgtgc gaacgtttcc caggtgatgc atatcatcgg tgacgttgca
    32401 ggtcgtgact gcgtactggt cgatgatatg atcgacactg gcggtacgct gtgtaaagct
    32461 gctgaagctc tgaaagaacg tggtgctaaa cgtgtatttg cgtacgcgac tcacccgatc
    32521 ttctctggca acgcggcgaa caacctgcgt aactctgtaa ttgatgaagt cgttgtctgc
    32581 gataccattc cgctgagcga tgaaatcaaa tcactgccga acgtgcgtac tctgaccctg
    32641 tcaggtatgc tggccgaagc gattcgtcgt atcagcaacg aagaatcgat ctctgccatg
    32701 ttcgaacact aatcgaaccc gctcaaagac ccgctgcggc gggttttttt gtctgtaata
    32761 tccatttgta tgacctatgc ctccttcacc tgccatttag ttgacagatg atgcgctcac
    32821 ggatgaaaca ttattgtgaa caaaatattt tcctcacatg tgatgccttt ccgcgctctg
    32881 atcgacgctt gctggaaaga aaaatatact gccgcacggt ttacccgtga cctgattgcc
    32941 gggataaccg tcgggattat tgctatcccg ctggcgatgg cgttggctat tggtagtggt
    33001 gtggcacccc agtacggttt atataccgca gctgttgcgg ggattgtcat tgctctgacg
    33061 ggtgggtcac gctttagcgt ttccggtccg actgcggcat ttgtggtaat tctctatccc
    33121 gtgtcgcaac agtttggact ggcaggactg ctggttgcga ccttgctgtc ggggatcttt
    33181 ttgattctga tgggtctggc acgctttggt cgcctgattg agtatattcc ggtttccgtc
    33241 accttaggtt tcacctcggg tatcgggatc accatcggta ccatgcagat taaagatttt
    33301 ctcggtctgc aaatggccca tgtcccggaa cattatctac aaaaagtcgg cgcattattt
    33361 atggcgctgc cgaccattaa tgtgggtgat gctgccattg gcattgtgac gctaggtatt
    33421 cttgtttttt ggccgcgtct gggcattcgt ttacccggtc accttccggc cttgctggct
    33481 ggttgcgcgg tgatggggat tgttaacctg ctcggcggac atgttgctac catcggttcg
    33541 caattccact acgtcctggc cgatggttct cagggtaacg gtattccgca actgctgccg
    33601 caactggtgc tgccgtggga tctgcctaat tcagaattca cgctaacctg ggattctatt
    33661 cgcacactgc tgcctgcggc attctcaatg gcaatgctcg gcgcaatcga atctctgctc
    33721 tgcgccgtgg tactggatgg tatgaccggg acgaaacaca aggcgaacag cgaactggtt
    33781 ggacagggac tggggaatat tatcgctccg ttctttggtg gtattaccgc tacagctgcc
    33841 atcgcgcgtt ctgccgctaa cgtccgtgcc ggggcaactt cccctatctc ggcggtgatc
    33901 cactctattc tggttattct tgccctgctg gtactggcac cgctgctctc ctggctgccg
    33961 ctttccgcta tggcagccct gctgttgatg gtggcgtgga acatgagtga agcgcataaa
    34021 gtggtcgact tgctgcgtca tgcaccgaaa gatgacatca ttgtcatgct gctgtgcatg
    34081 tcgctgaccg tgctgtttga tatggttatt gccatcagcg tggggatcgt gctggcatcg
    34141 ctgctgttta tgcgtcgtat cgcacgtatg actcgcctgg caccggtagt cgtagatgtt
    34201 ccagacgatg ttctggtact gcgcgttatt ggcccgctgt tttttgctgc tgctgaaggc
    34261 ttgttcacgg acctggagtc acgtcttgaa ggcaaacgga ttgtgattct gaagtgggat
    34321 gccgttccgg tacttgatgc tggtggtctt gatgcgttcc agcgttttgt gaagcgtctg
    34381 cccgaaggat gtgaactgcg cgtgtgcaac gtggaattcc agccactgcg cactatggct
    34441 cgcgcaggca ttcaaccgat cccgggacgc ctcgcgttct tcccgaatcg tcgcgcggcg
    34501 atggcggatt tataagtacg agattgacca gtcagcgcaa actgactggt cagcaaactg
    34561 cattatttgt tagctatgac ggcgattatc gctacggcgg caacgtttgt catagtgacg
    34621 aacccaccag taacggtcgc acacctgttc atgcccgcca acacgggcac ccgccagcca
    34681 gagaatggca ccgacgaaaa tacttagcac tgcaccatgt gcgaaatact ggggcatatt
    34741 aaactgtggt aactggttga ggattgaata ccccacgccg accaccatta ccaccagccc
    34801 caaccccatg agcacgttac cgagtaacga agcgtttttg cgtttcatat gtcacctccg
    34861 gaactttctg ggttgtaaca gggaataccc ctcttcctta tgtgtaaagt atagacaaca
    34921 ccctgtgggt tatgtgcggg cgtgatcaca attacaaccc ttatttcaac aaaactttac
    34981 aaataaacgc ctgaacacct tgactttcta atagtccact caggcaatta ttacgttatc
    35041 tgcatttgat acgcagtttt ttgtcaagcg gggccgcaac cagtaaacta cgcgccagtt
    35101 atgtacacac tcaggacaaa aaacgtgacg attaaattga ttgtcggcct ggcgaacccc
    35161 ggtgctgaat acgccgcaac gcgacataat gctggtgcct ggttcgttga cttactggca
    35221 gagcgtttgc gcgctccgct gcgcgaagag gctaaattct ttggttatac ttcgcgagtc
    35281 actcttggag gcgaagatgt ccgcctgtta gtcccgacta catttatgaa tctcagcggc
    35341 aaagccgttg cggcgatggc cagttttttc cgcattaatc cggacgaaat tctggtggcc
    35401 cacgacgaac tggatctgcc tcctggcgtc gccaaattta aattgggcgg tggccatggt
    35461 ggtcacaatg gactgaaaga catcatcagt aaattgggta ataaccctaa ctttcaccgt
    35521 ttacgcatcg gaatcggtca tcctggcgat aaaaataaag ttgtcggttt tgtgttaggc
    35581 aaaccgcctg ttagtgaaca gaagttaatt gatgaagcca tcgacgaagc ggcgcgttgt
    35641 actgaaatgt ggtttacaga tggcttgacc aaagcaacga accgattgca cgcctttaaa
    35701 gcgcaataag tcgttgtctg cggcattttt gccgagtgcc gtgtataata ggcaaagtta
    35761 tttccatttc tgcaatctgt tagcaataac aggttgatta ttaagatatt aaggtgattt
    35821 aaatcatggg attcaaatgc ggtatcgtcg gtttgcccaa cgtcgggaaa tctaccctgt
    35881 tcaacgcgct gaccaaagcc ggtattgaag cggccaactt tccattctgc accattgagc
    35941 cgaacacagg cgtcgtacca atgcctgacc ctcgcctgga tcaactggct gaaatcgtaa
    36001 aaccgcaacg tacgcttccc acgaccatgg aatttgtcga tatcgccggt ctggtaaaag
    36061 gcgcatcgaa aggcgaaggt ctgggtaacc agttcctgac caacatccgt gaaaccgaag
    36121 cgatcggtca cgttgttcgc tgctttgaaa atgacaacat cattcacgtt tccggcaaag
    36181 ttaacccggc tgacgatatt gaagttatca acaccgaact ggcgctggca gacctcgaca
    36241 cctgcgaacg tgcgattcat cgcgtacaga agaaagccaa aggtggcgat aaagacgcga
    36301 aagctgagct ggcggtccta gaaaaatgcc tgccccagct ggaaaacgca ggtatgctgc
    36361 gcgcgctgga tttaagcgct gaagagaaag cggctattcg ttacctgagc ttcctgacgc
    36421 tgaaaccaac aatgtacatc gccaacgtca acgaagacgg ttttgaaaac aacccatatc
    36481 ttgaccaggt gcgtgaaatc gcggcgaaag aaggttctgt tgtggttccg gtttgtgctg
    36541 ctgttgaagc agacattgcc gaactggacg acgaagaacg tgacgagttt atgcaggagc
    36601 ttgggctgga agaaccgggc ctgaaccgtg tgatccgtgt cggttataag ctgctgaacc
    36661 tgcaaactta cttcaccgct ggggtgaaag aagtgcgtgc atggaccatt ccggttggag
    36721 caaccgcgcc gcaggcagcg ggcaaaatcc atactgattt tgaaaaaggc tttatccgtg
    36781 cacaaaccat ctcgtttgaa gatttcatca cttacaaagg tgaacaaggc gcgaaagaag
    36841 caggcaaaat gcgtgcagaa ggtaaagatt acatcgttaa agatggcgat gtgatgaact
    36901 tccttttcaa cgtctaatta atttattgtc tcatgaggtt tcgctaaatc tcatgaagag
    36961 cgcaaaagta tataaaatcc acgcaattgc gtggattttt catttcaccg agtcctaatc
    37021 attcttgtaa catcctacac ttttgtgctt attgcctaca gtgaaccacg agaataatta
    37081 aagacatcag tgtcagatga cggatatctc attagtagtg atataacgta agtttttgtt
    37141 caactacaca tcaccccctt cacagctgat caatattgat cacgctccaa tatgctggcg
    37201 agaaaaggac ttacctgaag ggtaatatcc cgcagataaa gtccccgaat aacgtgatta
    37261 cgtgcgatcc tggcaagatg aaaatgataa ccagtagcac aataacaacg ataggccaga
    37321 aaacccattg accaaaaatg aatatcgatg ttttttgctt tttcttatta gtaatgcaaa
    37381 aatggcgcat attgtttttg ttatgcagat acatcaagcg aaattcccat aactaattag
    37441 gattattaca aaaaatcttc accaaatttt ttaaccgctg tttgttgtaa tatttttgcc
    37501 atatttatag taacgaattg gaataagtaa cgacaactta gggaataact cgatataaac
    37561 tcacctttgt aagggggatg ttaaatatat attaattcct gtgttcctga ttttgcccgg
    37621 taatagagaa ttattctctg agaagctcat catgattgca ccatacaaat atcatgatga
    37681 taagaggata ttattcattg atttaaacaa aatgacaaaa tccatagacc catagtctgg
    37741 gtactatata ggagtggtat gggcatcaaa caacacaatg ggaataccaa agcagatcgt
    37801 ctcgctgaat
//
LOCUS       DAETQN010000028        37769 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.028, whole genome shotgun sequence.
ACCESSION   DAETQN010000028 DAETQN010000000
VERSION     DAETQN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37769
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.028"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="K0W68_001436"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="K0W68_001436"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBI3073038.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="K0W68_001437"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="K0W68_001437"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBI3073039.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="K0W68_001438"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="K0W68_001438"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBI3073040.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="K0W68_001439"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="K0W68_001439"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBI3073041.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="K0W68_001440"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="K0W68_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HBI3073042.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="K0W68_001441"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="K0W68_001441"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBI3073043.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="K0W68_001442"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="K0W68_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBI3073044.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="K0W68_001443"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="K0W68_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBI3073045.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="K0W68_001444"
     CDS             6537..6773
                     /locus_tag="K0W68_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBI3073046.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="K0W68_001445"
     CDS             6776..7633
                     /locus_tag="K0W68_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBI3073047.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="K0W68_001446"
     CDS             7654..9645
                     /locus_tag="K0W68_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBI3073048.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="K0W68_001447"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="K0W68_001447"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBI3073049.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="K0W68_001448"
     CDS             complement(10248..11144)
                     /locus_tag="K0W68_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBI3073050.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="K0W68_001449"
     CDS             complement(11193..11432)
                     /locus_tag="K0W68_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBI3073051.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="K0W68_001450"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="K0W68_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBI3073052.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="K0W68_001451"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="K0W68_001451"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBI3073053.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="K0W68_001452"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="K0W68_001452"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBI3073054.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="K0W68_001453"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="K0W68_001453"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBI3073055.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="K0W68_001454"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="K0W68_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="K0W68_001455"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="K0W68_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBI3073056.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="K0W68_001456"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="K0W68_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBI3073057.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="K0W68_001457"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="K0W68_001457"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBI3073058.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="K0W68_001458"
     CDS             complement(21570..22739)
                     /locus_tag="K0W68_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3073059.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="K0W68_001459"
     CDS             complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="K0W68_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBI3073060.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="K0W68_001460"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="K0W68_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBI3073061.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /locus_tag="K0W68_001461"
     CDS             complement(24315..25247)
                     /locus_tag="K0W68_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3073062.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /locus_tag="K0W68_001462"
     CDS             25360..26571
                     /locus_tag="K0W68_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBI3073063.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="K0W68_001463"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="K0W68_001463"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBI3073064.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="K0W68_001464"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="K0W68_001464"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBI3073065.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="K0W68_001465"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="K0W68_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBI3073066.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="K0W68_001466"
     CDS             complement(30320..31576)
                     /locus_tag="K0W68_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBI3073067.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="K0W68_001467"
     tRNA            31884..31960
                     /locus_tag="K0W68_001467"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="K0W68_001468"
     tRNA            31965..32041
                     /locus_tag="K0W68_001468"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="K0W68_001469"
     CDS             32149..32454
                     /locus_tag="K0W68_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBI3073068.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="K0W68_001470"
     CDS             32580..34184
                     /locus_tag="K0W68_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBI3073069.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /locus_tag="K0W68_001471"
     CDS             complement(34196..35008)
                     /locus_tag="K0W68_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073070.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /locus_tag="K0W68_001472"
     CDS             complement(35012..35797)
                     /locus_tag="K0W68_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBI3073071.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     VVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="K0W68_001473"
     CDS             complement(35794..36462)
                     /locus_tag="K0W68_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBI3073072.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="K0W68_001474"
     CDS             complement(36526..37164)
                     /locus_tag="K0W68_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBI3073073.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..>37769)
                     /locus_tag="K0W68_001475"
     CDS             complement(37177..>37769)
                     /locus_tag="K0W68_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HBI3073074.1"
                     /translation="GLPLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLR
                     VCLHNAVTLCNWVWPMTVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIF
                     TLHRAYTVKLMQTKDMRNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYK
                     EMGWDPQLGCPTRETLQRLGLEDIAADLAAHNLLPA"
BASE COUNT         9023 a         9930 c         9381 g         9435 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca ctgtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacgaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaacca cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagccgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccgg
//
LOCUS       DAETQN010000029        37257 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.029, whole genome shotgun sequence.
ACCESSION   DAETQN010000029 DAETQN010000000
VERSION     DAETQN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37257
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.029"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..642)
                     /locus_tag="K0W68_001476"
     CDS             complement(<1..642)
                     /locus_tag="K0W68_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HBI3073075.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
     gene            complement(742..1653)
                     /gene="gsiD"
                     /locus_tag="K0W68_001477"
     CDS             complement(742..1653)
                     /gene="gsiD"
                     /locus_tag="K0W68_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBI3073076.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1656..2576)
                     /gene="gsiC"
                     /locus_tag="K0W68_001478"
     CDS             complement(1656..2576)
                     /gene="gsiC"
                     /locus_tag="K0W68_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBI3073077.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2594..4132)
                     /gene="gsiB"
                     /locus_tag="K0W68_001479"
     CDS             complement(2594..4132)
                     /gene="gsiB"
                     /locus_tag="K0W68_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBI3073078.1"
                     /translation="MAKAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4152..6023)
                     /gene="gsiA"
                     /locus_tag="K0W68_001480"
     CDS             complement(4152..6023)
                     /gene="gsiA"
                     /locus_tag="K0W68_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBI3073079.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6010..6975)
                     /gene="iaaA"
                     /locus_tag="K0W68_001481"
     CDS             complement(6010..6975)
                     /gene="iaaA"
                     /locus_tag="K0W68_001481"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBI3073080.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7179..8414
                     /gene="moeA"
                     /locus_tag="K0W68_001482"
     CDS             7179..8414
                     /gene="moeA"
                     /locus_tag="K0W68_001482"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBI3073081.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8414..9163
                     /gene="moeB"
                     /locus_tag="K0W68_001483"
     CDS             8414..9163
                     /gene="moeB"
                     /locus_tag="K0W68_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBI3073082.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9342..10004)
                     /gene="fsa"
                     /locus_tag="K0W68_001484"
     CDS             complement(9342..10004)
                     /gene="fsa"
                     /locus_tag="K0W68_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBI3073083.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10135..11034
                     /locus_tag="K0W68_001485"
     CDS             10135..11034
                     /locus_tag="K0W68_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBI3073084.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11040..13472
                     /locus_tag="K0W68_001486"
     CDS             11040..13472
                     /locus_tag="K0W68_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBI3073085.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13618..14433
                     /gene="ybiV"
                     /locus_tag="K0W68_001487"
     CDS             13618..14433
                     /gene="ybiV"
                     /locus_tag="K0W68_001487"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBI3073086.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14585..15850
                     /locus_tag="K0W68_001488"
     CDS             14585..15850
                     /locus_tag="K0W68_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBI3073087.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15817..16977)
                     /locus_tag="K0W68_001489"
     CDS             complement(15817..16977)
                     /locus_tag="K0W68_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI3073088.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17215..18516
                     /locus_tag="K0W68_001490"
     CDS             17215..18516
                     /locus_tag="K0W68_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3073089.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18531..20894
                     /locus_tag="K0W68_001491"
     CDS             18531..20894
                     /locus_tag="K0W68_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HBI3073090.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21074..22669)
                     /locus_tag="K0W68_001492"
     CDS             complement(21074..22669)
                     /locus_tag="K0W68_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBI3073091.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22888..23808
                     /gene="ldtB"
                     /locus_tag="K0W68_001493"
     CDS             22888..23808
                     /gene="ldtB"
                     /locus_tag="K0W68_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBI3073092.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23867..24985)
                     /locus_tag="K0W68_001494"
     CDS             complement(23867..24985)
                     /locus_tag="K0W68_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBI3073093.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24982..25449)
                     /gene="mntR"
                     /locus_tag="K0W68_001495"
     CDS             complement(24982..25449)
                     /gene="mntR"
                     /locus_tag="K0W68_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBI3073094.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25635..25763
                     /gene="mntS"
                     /locus_tag="K0W68_001496"
     CDS             25635..25763
                     /gene="mntS"
                     /locus_tag="K0W68_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBI3073095.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26035..27618
                     /gene="opgE"
                     /locus_tag="K0W68_001497"
     CDS             26035..27618
                     /gene="opgE"
                     /locus_tag="K0W68_001497"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBI3073096.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27667..28182)
                     /gene="ompX"
                     /locus_tag="K0W68_001498"
     CDS             complement(27667..28182)
                     /gene="ompX"
                     /locus_tag="K0W68_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBI3073097.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28235..28300)
                     /locus_tag="K0W68_001499"
     CDS             complement(28235..28300)
                     /locus_tag="K0W68_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073098.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28535..29422
                     /gene="rhtA"
                     /locus_tag="K0W68_001500"
     CDS             28535..29422
                     /gene="rhtA"
                     /locus_tag="K0W68_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBI3073099.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29721..30224
                     /gene="dps"
                     /locus_tag="K0W68_001501"
                     /gene_synonym="pexB"
     CDS             29721..30224
                     /gene="dps"
                     /locus_tag="K0W68_001501"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBI3073100.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30628..31374
                     /gene="glnH"
                     /locus_tag="K0W68_001502"
     CDS             30628..31374
                     /gene="glnH"
                     /locus_tag="K0W68_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBI3073101.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            31513..32172
                     /gene="glnP"
                     /locus_tag="K0W68_001503"
     CDS             31513..32172
                     /gene="glnP"
                     /locus_tag="K0W68_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBI3073102.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            32169..32891
                     /gene="glnQ"
                     /locus_tag="K0W68_001504"
     CDS             32169..32891
                     /gene="glnQ"
                     /locus_tag="K0W68_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBI3073103.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            33008..35233
                     /gene="ybiO"
                     /locus_tag="K0W68_001505"
     CDS             33008..35233
                     /gene="ybiO"
                     /locus_tag="K0W68_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HBI3073104.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(35230..36156)
                     /gene="rlmF"
                     /locus_tag="K0W68_001506"
     CDS             complement(35230..36156)
                     /gene="rlmF"
                     /locus_tag="K0W68_001506"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBI3073105.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            36432..36692
                     /locus_tag="K0W68_001507"
     CDS             36432..36692
                     /locus_tag="K0W68_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI3073106.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36957..>37257
                     /locus_tag="K0W68_001508"
     CDS             36957..>37257
                     /locus_tag="K0W68_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBI3073107.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         9032 a        10058 c         9338 g         8829 t
ORIGIN      
        1 caaatcgtga tctttgccgt tgatacgtag cgtaaaagag atacattttt tacccggacg
       61 atcaaagcaa ttgtatgccg gattccagct gttgttagag tcatctgaag ccaccgacca
      121 cggtcgtttc gatgggttaa aatcggcaaa aataacccgc cctgactcgt cgacaagcgg
      181 tccatttact tgttcctgat gataaacaac gattttatca ttcgccctcc gcgaaaagag
      241 ataatattta ttatcatcaa gtaccagtcc caccgagtct aaaaatggca tcatttccat
      301 tttcagaatc acggcgcgtt gaatctcctc tgtatttttt aataaataca gtgagtcctt
      361 taatgtcagg gccgaacgtt caatatcttt aaagaaatag ccaacgtttt tctgaataag
      421 ttcaaagcgg ctatcggcac gttttaccgc gcgttccgat accagttgat agttaatgat
      481 gaaaaaaaga ccaataaatg acagtgctgc caataaaaat aaaattatca gccttatctt
      541 tatcttttcg tataaactca gcatgttaat acatttaaca aaataactat ctgaacgctg
      601 tgccgctgca aaaatagtcc cttgctgcgg ctttttaacc aaattacccg attctacaaa
      661 atcagcagaa agttacaaca cttgcttagg gcaataattt cctgtgaggt gattaccctt
      721 tcaagcaata ttcaaacgta actatccttt aattttcgga tccagcgcat cgcgtaaacc
      781 atcgcccaac aaattgaacg ccagtacggt cagaaaaata gccagggccg gaaaaacagc
      841 gacatgcggc gcgataacca tatccgctcg tgcctcattg agcattgctc cccactctgg
      901 tgtcggcggc tgcgcaccga ggccgagaaa tgacaggctg gcggcagaga taatcgaggt
      961 gccaatgcgc atggtgaaaa acaccacgat agacgagacg gtcccaggca agatatgacg
     1021 caataaaatg gtcatatcgc tggcaccaat actgcgtgct gactcaataa aggtttgctg
     1081 tttcaacacc agcgtgttgc cgcgcaccag tcgggcaaac gcggggatgg aaaaaatggc
     1141 gactgcgata atcacgttag caatgccgct tcccaacacc gcaacaacag cgatcgccag
     1201 taaaataccc gggaaggcaa acagcacatc gcaaatgcgc atgatcagcc gatcccacca
     1261 gccttcataa tatccagcca acaagcccag caacgtcccg atcgccgcac cgataaacac
     1321 ggcaaacacg cccgccgcca gcgagatttg cgcaccaacc aggacacggc tgaaaatgtc
     1381 gcgccccagt gaatcgacgc caaaccagtg ctgcaaagaa ggtccgttat tcagattgtc
     1441 ataatcaaaa taattttcgg cgtcataggg agcgatccag cgtgcaaaaa tggccaccac
     1501 aatcaataaa ataacgaata atgcggcggt catcgccata tgctgacggc gaaatcgtcg
     1561 ccagaattca tgccacggtg tacgcacctg gtcaggtttg accagtggca tggcgtttaa
     1621 caccgcctga cgtcgccagt taaatagtcg catccttact tgtacctgat agccgggtta
     1681 atggcggcgt aaagcacatc caccactaag ttgataagaa taaattccag cgagaaaagc
     1741 agaatttccg cctgaatcac cgggtaatcg cgcatttcta cggagtcaac gagtaagcgt
     1801 ccaagtccgg gccagttgaa aactttctcc accacgatag aaccaccgag caaaaagcca
     1861 aactgtaagc ccatcatggt cactaccggg atcatcgcgt tacgtagccc gtgtttgagg
     1921 acaacccagg tttcgctcac ccctttcgcc ctcgcggtac gcatataatc ttcgcttaaa
     1981 acatcgacaa acgacgcgcg ggtaaagcgc gccatcacgg ctgccactgc cgcgccgagc
     2041 gtcagggagg gtaaaatgta gtgctgccaa ctgtctgctc ccacggtagg cagccagccc
     2101 agttcaacgg agaatacctg aattaaaagc atccccagcg caaatgccgg aaacgagatc
     2161 cccgacaccg caatggtcat actcaatcga tccggccaac gattacgcca gacggcggcg
     2221 ataattcccg ccgccatacc aaatatcacc gcccagacca tactggttat ggtcagccac
     2281 agcgttggca taaagcggct ggcaatctca tcggcaaccg gacgacgcga caccatcgac
     2341 aggccaaaat ccccctgcac agcattgctg atatagtgcc agaactggtg atacagcggc
     2401 tgatccaacc ccagctgctg acgcaccagt tctataacct gcgcatcagc ttcgagcccg
     2461 gcaatcaatc gcgccggatc gccgggcagc atatgcacaa ataaaaacac cagcaccgag
     2521 acgataaaca gcgtcggaat caaccccagt aagcgtttga taacgtaatt aagcattcca
     2581 ctccctgcgt tgcttattgc aaatccgcgt cttcaaagct gaagccggtg tctggcatga
     2641 tccaaaaacc ggtcaggttt ttactgtgtg ccgacaccag tttttctacc accagcggga
     2701 tccacggcga ctcttgccag atgatatcct gcgccgcctt atacaagcgg gtcttttccg
     2761 ccggatcatt agttttcagt gcctgagcca ggaagtcgtc cacctgttta ttgctgtaaa
     2821 atgcggtatt aaacagcgtc ggtggccagt tttgcgaggc gaacagtggc gatagcgccc
     2881 agtccgcttc gccggttgaa gccgaccagc cagtgtagaa catccgcacg ccgctctctt
     2941 tttgcccttt accttcaact tctgccgccc gctgtccggc atccatcgcg gtcacctggg
     3001 ctttaatccc gacctgcgct aactgctgct gggtaaattg cagcactttc tgcgcggtgc
     3061 tgtggttatg tgacgaccac agcgtggtac tgaaaccgtt gggatatccc gcctctttca
     3121 gtaattcgcg cgctttcact ggatcgtaag gccacggttt ataactttgc gcgtaggcga
     3181 tacttggcgg taccacacca gtagctggcg ttgcatagcc cgcaaaggca actttcacca
     3241 gcgccggacg gttaatggcg taattcagcg cctcacggac cttcgggtta tcgaacggct
     3301 tttgcgtcac gttcatactg atataacgct gcataattga cggactggcc atcaactcga
     3361 tatttttgtt tttctccagc agtgtggcct gctcgtaggg aatggggaaa gcaaactgcg
     3421 cttcaccggt ttgcagcatt gccgcgcggg tgttgttatc cgccaccgga cgccaggtta
     3481 tgctgtccag tttgggcaat cctggctgcc agtaacccgc gaattttttc accttcacaa
     3541 aatcggtctg attccaggta tccagttcat acggtccggt tcccaccgga tgaaaaccaa
     3601 tctccttgcc atatttttcc agcgctgccg gtgaaatcat cgcggtcgcc ggatgggcaa
     3661 gaatattaat aaacgctgag aacggctgtt tgagggtaat ctttaccgtt gtcggatcga
     3721 tcgcctccgt tttggcaata ttcttataca ggttatagcg tttaagatga ttcgccggat
     3781 cgctggcccg gtccagattc gcttttaccg ccactgcgtt gaaatcggtg ccatcctgga
     3841 atttaatgcc ttcccgcaat ttcacggtgt aagtaatgcc gtcatcggaa acggtataac
     3901 tctccgccag cacgtttttc agtttcatct ctttatccag accgaacagc ccctggtaaa
     3961 acgatttcgc tacggcctga gataacgtat cattcgcgtc atacggatcg agcgtggtga
     4021 aattcgatcc taccgccacc accacatctt tggcagcgaa tgcacaagat gccatcaacg
     4081 ctgtcgcaat gcccagcgcc actaacccac tacggtgtac agcttttgcc attttattct
     4141 ccgcctgaat gttatctacg catgaatgcg tattctgatt gtggttgtgc gacgtaatgc
     4201 cccggcccga cgcattgcaa cgagacggct gccacctctt cgccacgcag atgaatattg
     4261 ctgggaagat cgtccgacag cagtacacgc tgcggtcgtt gtcgggacgg ttcagcgacc
     4321 ggaactgccg ccagtaattt acgcgtataa ggatgctgcg ggttttcgaa gaccgcgcgc
     4381 cgtggaccaa tttcaacaat ttgcccgaga tacatcaccg ccacacgatg actaatccgc
     4441 tctaccacgg ccatatcgtg ggagataaac agatacgcaa tgccgaaatc acgctggaga
     4501 tcgagcaaca agttgataat ctgcccgcga atagaaacat ccagcgccga aacggcttcg
     4561 tcggcaatga tcacttttgg attcaatgcc aacgcgcgag caatgcagat gcgctggcgc
     4621 tgaccgccgg aaaactcatg cgggtaacgc caggcatgtt caggtaacag gcccacgcgc
     4681 tccagcaacc acgcaacgcg tgcagccgcg tctttacctg gcaataaacc gtgtacacgc
     4741 agcggttcga taatcgaatc accgatggtc tgacgtgggt ccagcgaagc gtaagggtcc
     4801 tgaaaaataa actgaatatc ccggcgcaat gcctgaagtt tgccgggtga caaggtatcg
     4861 attctctgac cgttaaagat aatttcgccg ccctgcgatt cgaccaggcg cagcaacgcc
     4921 cgcccggtag tggatttacc gctgccagac tcgcccacca gcgatagcgt ttcgccaggc
     4981 cagagatcaa aactgacttt ctcaacggca tgcacttccc gcgttacgcg attcaacaaa
     5041 ccgctgcgca aagggaaacg ggtgacaaga ttacgcactc gtaaaacagg ttcgccatcc
     5101 accaccgttt tctgctcgat ggggggggcc tgtttcgctg gatgttcaag cgatatcaac
     5161 gggaaacgtc ggggataatc caaccctttc atcgcaccaa gttgcggaac agcagctaac
     5221 agcgcacggg tgtaaggatg ttgcggtgca tgaaaaatct gttcgacggt acccgtttcc
     5281 accgcctcgc cctgatacat caccagtacc cgatcggcaa tctctgccac cacgcccata
     5341 tcgtgagtga taaagataac gcccatcgac atctcttttt gcaatacttt gattaattgc
     5401 aggatctgcg cctgaatagt gacatccagc gcggtggttg gctcatcggc aatcagcacc
     5461 gccgggcggc atgacagcgc catcgcaatc atcactcgct ggcgcatccc gccagagagt
     5521 tgatgcggat aacgtgaaag aatggtttgt gcctcaggaa tgcgtacctg atccagcatc
     5581 cgcttcgcct cgaccatcgc ttcttcacga ctggcgttct gatgcagacg aattgattcg
     5641 gcaatctgtt cacccacagt aaataccggg ttcagcgatg tcatcggctc ctgaaatatc
     5701 atcgccatat ccgcaccgcg aacatggcgc atttgtgcag cgctctgctc gctaagttca
     5761 atcacttcgc gactgcgccg ctgcaacagc attttatcgc actgtactaa accgcccgcc
     5821 tgttccaaca ggcgcatcaa tgccaacgca gtcactgact taccggagcc ggattcgcca
     5881 acaattgcca gcgtctcacc gcgttgcaga ctaaaagaga gattgcggac cgcagctatt
     5941 ttctgctggt cctgcataaa ggcaatattc agattttcaa ccgccagcac attaccggca
     6001 tcaagttcat cactgtgtgg caacggtgtc ccctttttca cggtagatac cggtggttgg
     6061 cgtatcgcct gcgtagcccc aggcgcgata cattccttcg gtgttaaacg gtagcgcgac
     6121 attcccttca tggtcgatag cgattaagcc accgctaccg ccaagcgcag ggagtttttc
     6181 cattactacc cgctcgcagg cttccgcgag acttaatccg ccgtaatcca ttaacgcggc
     6241 gatgtcatat gccgccagcg cgcggatgaa gacttcgccc gtgccgatac aagaaaccgc
     6301 cacactggcg ttattggcgt agcatccggc acccactaag ggactatcgc caactcgtcc
     6361 gggtaattta ttggtcattc cgcctgtgga cgtggctgcc gccaaattgc cgtctaaatc
     6421 caacgccacg gcccccacgg tgcccatttt ctgtttttca tccagtggcg caccgctatg
     6481 gtcgaggacg gttgcccctt ccttgcgcgc tgccagtagt tgttcataac gcaaaggcgt
     6541 ggagaaaatc tccggcgaga cgcgctccat gccacgagca aacgcaaaat tttctgcccc
     6601 ttcgccaatc atcatcacat gcgggctttg ctccatcacc agccgggcgg caagaaccgg
     6661 attacgcaga tgactaacgc ccgccaccgc accggctttc agggtgttac catccatcac
     6721 acaggcgtcc agttcatggg tttcatcacg cgtaaagaca gcgccaattc cggcgttaaa
     6781 cagtggacac tcttccagca gacgcaccgc ttccgtcacc acatccagcg cactttcgcc
     6841 cgcttccagc attttctgcc cggtttcaac aatggcagac aacgcctcaa tgtagcgtaa
     6901 ttcctgttgc agactcatct gcgcgcggct aattgcacct gcgccaccat gaattgcaat
     6961 gactgctttg cccatagtat tcgtccatta agggtggctc gccacttttg ctataaatat
     7021 cagaattttt atgaattact cattcaattt ttgaatatag aaagatggat atggcatgta
     7081 aaggcaggag ccaacgctac gcccgaggtg gcatacagtc tgtggcgttt ttctgacata
     7141 ataggcaaat tcgattttgc ctccgcagga gtgttttcat ggaatttacc accggattga
     7201 tgtcgctcga caccgcgctt aatgagatgc tttctcgcgt caccccactg accgcccagg
     7261 aaacgctgcc actggtacag tgttttggtc gtattctggc gagcgatgtc gtttcgccgc
     7321 ttgatgttcc ggggtttgat aactccgcaa tggacggcta cgcggtgcgt ttagccgata
     7381 ttgcctccgg gcaaccgctg cccgttgccg gtaaatcctt tgccggtcag ccgtatcatg
     7441 gtgaatggcc tgcgggcacc tgcattcgta ttatgaccgg tgcgccggtg ccggaaggct
     7501 gcgaagcggt ggtgatgcag gagcagactg aacaaacgga caatggcgtg cgttttactg
     7561 ctgaagtgcg tagcgggcaa aatattcgcc gtcgcggtga agatatctct gcaggtgcgg
     7621 ttgttttccc ggcgggaact cgcctgacta ccgcagagct gccagtaatt gcttcactgg
     7681 ggattgccga agttccggtg attcgtaaag tgcgtgtagc gcttttttct accggtgatg
     7741 aactccagtt gcccggtcag ccgctgggcg acggccaaat ctacgacacc aaccgtctcg
     7801 ccgtacacct gatgttagaa cagctgggtt gcgaggtaat caacttaggg attatccgcg
     7861 acgatcccca tgccctgcgc gccgcattta ttgaagccga cagccaggcg gatgtagtga
     7921 tcagttccgg cggtgtttca gtgggtgaag cggattacac caaaacgatt cttgaagagc
     7981 tgggggagat cgccttctgg aagctggcga ttaaaccagg taaaccgttc gcgttcggta
     8041 aactcagcaa tagctggttc tgcggcctgc cgggcaaccc ggtttcagcg acgctgacct
     8101 tctatcaact ggtacagcct ttgctggcaa aactaagcgg caacaccgcc agcggcctgc
     8161 ccgcgcgcca gcgcgtacgc acagcgtccc gcctgaaaaa aacgccagga cgtcttgatt
     8221 tccagcgcgg cgtgctgcaa cgcaacgccg acggtgaact tgaagtgacg accaccggac
     8281 atcagggttc acatatattt agctccttta gtctcggcaa ctgctttatc gtgctggaac
     8341 gcgatcgcgg caatgtggac gtgggcgaat gggtggaagt agaaccgttt aacgcgttgt
     8401 tcggaggcct gtaatggcgg aactcagcga tcaggagatg ctgcgctaca accggcaaat
     8461 cattctgcgc ggttttgatt ttgacggtca ggaggcgcta aaagattctc gtgtgctgat
     8521 cgtcggcctc ggtggactcg gctgcgcagc ttcgctgtat ctggcaagcg ccggtgtcgg
     8581 caacctgacg ctgctcgact tcgacacggt ttcgctctcg aatctgcaac gccagacact
     8641 gcacagtgat gccacggtcg ggcaaccgaa ggtggaatcc gcccgtgacg ccctgacgcg
     8701 gatcaaccca catatcgcga ttacgccagt caatgcactg ctagatgacg cagaacttgc
     8761 agcattgatt gctgaacacg atctggtgct cgactgtacg gataacgttg cggtacgtaa
     8821 tcaactgaac gcaggctgtt ttgccgcgaa ggtgccgctg gtttccggcg cggcaattcg
     8881 tatggaaggg caaatcacgg tcttcactta tcaggacggc gaaccgtgct atcgctgcct
     8941 tagccgtttg tttggcgaga acgcattaac ctgcgtagaa gcaggcgtga tggcaccgtt
     9001 gatcggcgta attggttcgt tgcaggcaat ggaagcgatc aaactgctgg caggttatgg
     9061 caaaccgacc agcgggaaaa tcgtcatgta cgatgcgatg acctgtcagt ttcgcgaaat
     9121 gaaactgatg cgcaatccgg ggtgtgaggt gtgcgggcag taattgccac ctccaggcct
     9181 gataagacgc gacaagcgtc gcatcaggca tttgctccag acgccagatg cggcgtgaac
     9241 gccttatctg gcctacggtt cgggcacaga tttccaagcg cggcaggcat cggtgcactc
     9301 cgccacgtca tcagcaattt atgaggatgc agaacgtccg gttaaatcga cgttctgcca
     9361 aacgctccct gccagtcctg ctcaaacttc gccacagcgg cttccaccgc cggatagcta
     9421 atcatctgtt gtgccacatc cagtggcaga gtaattgatt cacatcctgc cagtaagcag
     9481 tccagcgcct gacgcggggt tttgaaactc gctgccagca ctttcgcctg cggcgcatgc
     9541 attttcaata actggtgtaa gtcggtcaca gtctgaatgc cgctaccgcc ctgagcatca
     9601 atacgattaa cgtaaggcgc aacatattcc gcacctgcca gcgccgacag caacccttgt
     9661 gctgcgccat ataccgcggt tcccagcgtt ggaatccctt ccgcttttaa catcttaata
     9721 gctgccagcc cctcggcggt caccggaact ttcaccacga tatccgcaat aatagaacgc
     9781 aatttacgtg cgtcattcac catcccttca gcagtggtag ccattacctg ggcaaacaga
     9841 cgcccctgac cgcccatcgc ttcatgaagt tgcggaagca caacatccag cggtttttta
     9901 cccgcggcga taatgcttgg gttagtggtc acacccgcca gcggaaaaat acgtgacagc
     9961 gccttcaccg caacaacgtc tgaagtatcc agatacagtt ccataaccat cctcaaaatg
    10021 tactttttgt ctgaatgtag actatcacgg gaaaactctc tgaacagttg acgcacatca
    10081 atctaacttt cattcgaaag tattttaatg tttgaacgaa acagagaggc gactatgatt
    10141 ttcaatattc agcgctactc gacccatgat ggccccggta tccgcacggt cgtatttctt
    10201 aaaggctgtt cgctgggctg ccgctggtgt cagaacccgg aaagccgcgc ccgcacgcag
    10261 gatctgctgt atgacgcacg actgtgtctg gaaggctgcg agctgtgcgc taaggccgcg
    10321 ccggaagtga ttgagcgcgc gctgaatggt ttgcttattc atcgggaaaa gttaaccccg
    10381 gagcatctga cggcgttaac cgactgctgt ccgacacagg cattaaccgt gtgtggtgaa
    10441 gtgaaaagcg ttgaggagat catgacgacc gttctgcgcg ataaaccgtt ttacgatcgc
    10501 agcggcggcg gtttaacgct ttcgggtggt gagcccttta tgcagccgga aatggcgatg
    10561 gcgctactgc aagccagcca cgaggcaggc attcatactg cggtagaaac ctgtctgcat
    10621 gtgccgtgga aatatatcgc cccttctctg ccctatatcg atctgtttct tgccgattta
    10681 aaacacgttg ccgacgcgcc gtttaaacag tggaccgacg gtaacgccgc cagagtgctg
    10741 gataacctga aaaaactcgc cgcagcgggc aaaaaaatca ttatccgcgt gccgctgatt
    10801 cagggcttta atgccgacga aacctctgta aaagctatta ccgattttgc cgccgacgag
    10861 ctgcacgttg gcgaaattca tttcctgccc taccacacgc taggcatcaa caaatatcac
    10921 ttacttaatc tgccctatga cgccccggaa aaaccgcttg atgcgccaga actgctcgac
    10981 tttgcccagc agtatgcctg ccagaaaggg ttaaccgcga ccttacgagg ataacaacca
    11041 tgaccacact gaaactggac acgctcagcg accgcattaa agcgcacaaa aatgcgctgg
    11101 tgcatattgt gaaaccgcca gtctgtaccg agcgcgcgca gcactatacc gagatgtatc
    11161 aacaacatct cgataagccg atcccggtac gtcgcgcgct ggcactggcg catcacctgg
    11221 cgaatcgcac catctggatc aaacacgatg agttgatcat tggcaaccag gcaagcgaag
    11281 ttcgcgccgc gccgatcttc ccggaatata ctgtctcgtg gatcgaaaaa gagattgatg
    11341 atctggcaga tcgtcccggt gctggctttg cggtgagcga agagaacaaa cgcgttctgc
    11401 atgaagtgtg cccgtggtgg cgcggtcaga ccgtacagga tcgctgctac ggcatgttta
    11461 ccgatgagca aaaaggtctg ctggcgaccg gaatcattaa agcggaaggc aatatgacct
    11521 ccggcgatgc gcacctggcg gtgaatttcc cgctgctgct ggaaaaaggt cttgatggtc
    11581 tgcgcgagaa agtagcggaa cgtcgctcgc gcatcaatct gacggtactg gaagatttgc
    11641 acggcgaaca gttcctgaaa gcgattgata tcgtgctggt ggcagtcagc gaacacattg
    11701 aacgttttgc agccctggcg cgtgaaatgg ccgcgaccga aacccgcgaa agccgtcgcg
    11761 atgaactgct ggcgatagca gaaaactgcg atcttatcgc ccaccagccg ccgcagactt
    11821 tctggcaggc gctgcaactg tgttacttca tccaattgat attgcagatc gaatctaacg
    11881 gtcactcggt atcgtttggt cgtatggacc agtatctcta cccgtactat cgccgcgacg
    11941 ttgaactcaa ccagacactg gatcgcgaac acgccatcga attgctgcat agctgctggc
    12001 tgaaactgct ggaagtgaac aagatccgct ccggctcaca ctcaaaagcc tctgcgggaa
    12061 gtcctctgta tcagaacgtc accattggcg gacaaactct ggttgatggt caaccaatgg
    12121 acgcggtgaa tccactctct tacgctatcc tcgaatcctg cggtcgcctg cgttccactc
    12181 agcctaacct cagcgtgcgt taccatgcag gaatgagtaa tgatttcctc gacgcctgcg
    12241 tacaggtgat ccgttgcggc ttcgggatgc cggcgttcaa caacgacgaa atcgtgatcc
    12301 cggaatttat taaactcggt attgaaccgc aggacgctta cgactacgca gcgattggtt
    12361 gtatcgaaac cgccgtcggt ggcaaatggg gctatcgctg taccggcatg agctttatca
    12421 acttcgcccg cgtgatgctg gcggcgctgg aaggcgggcg tgatgccacc agcggcaaag
    12481 tgttcctgcc acaagaaaaa gcgttgtcgg caggcaactt taacaatttc gatgaagtaa
    12541 tggacgcgtg ggatacgcaa atccgttatt acacgcgtaa atcaatcgaa atcgaatatg
    12601 tcgtcgacac catgctggaa gagaacgtgc acgatattct ctgctcggcg ctggtggatg
    12661 actgtattga gcgagcgaaa agtatcaagc aaggcggcgc gaaatatgac tgggtttctg
    12721 gcctgcaggt tggcattgcc aacctcggca acagcctggc ggcagtgaag aaactggtat
    12781 ttgaacaggg cgcgattggt cagcaacagc tggctgccgc actggcggat gacttcgatg
    12841 gcctgactca tgagcaactg cgtcagcggc tgattaacgg tgcgccgaag tacggcaacg
    12901 acgatgatac cgtcgatacg ctgctggctc gcgcttatca gacctatatc gacgaactga
    12961 aacagtacca taacccgcgc tacggtcgtg gtccggttgg cggcaactat tacgcgggta
    13021 cctcgtctat ctccgctaac gtaccgtttg gcgcgcaaac tatggcaaca ccggacgggc
    13081 gtaaagccca caccccgctg gcagaaggcg caagcccggc ctccggtact gaccatcttg
    13141 gccctactgc ggtcattggc tcagtgggta aactgcctac ggcagcgatt ctcggcggcg
    13201 tgttgctcaa ccagaaactg aatccggcaa cgctggagaa cgaatctgac aagcagaaac
    13261 tgatgatcct gctgcgtacc ttctttgaag tgcataaagg ctggcatatt cagtacaaca
    13321 tcgtttcccg cgaaacgctg ctggaggcga aaaaacatcc ggatcagtac cgcgatctgg
    13381 tagtgcgtgt cgcgggctat tccgccttct tcaccgcgct ctctccagac gcacaggacg
    13441 atatcatcgc ccgtactgaa catatgctgt aatctcctcg gcccggcggc gtcgtcgccg
    13501 ggctaaatca ttcacatcat caatttcatc cttactttca ttcgaaatat aatttgtgct
    13561 ctgcgtcaca ttgttattac actgttacgg tttcattttc gagccaggag cacacctatg
    13621 agcgtaaaag ttatcgtcac agacatggac ggtacttttc ttaacgacgc caaaacgtac
    13681 aaccaaccac gttttatggc gcaatatcag gaactgaaaa agcgcggcat taagttcgtt
    13741 gtcgccagcg gtaatcagta ttaccagctt atttcgttct ttcctgagct aaaggatgag
    13801 atctcttttg tcgcggaaaa cggcgcactg gtttacgaac atggcaagca actgttccac
    13861 ggcgaactga cccgacatga atcgcggatt gttattggcg agttgctaaa agataagcaa
    13921 ctcaattttg tcgcctgcgg tctgcaaagt gcatatgtca gcgaaaatgc ccccgaagca
    13981 tttgtcgcac tgatggcaaa acactaccat cgcctgaaag ctgtaaaaga ttatcaggag
    14041 attgacgacg tactgttcaa gttttcgctc aacctgccag atgaacaaat cccgttagtg
    14101 atcgacaaac tgcacatagc gctcgatggc attatgaaac ccgtcaccag tggttttggc
    14161 tttatcgacc tgattattcc cggtctacat aaagcaaacg gtatttcgcg gttactgaaa
    14221 cgctgggatc tgtcaccgca aaatgtggta gcgattggcg acagcggtaa cgatgcggag
    14281 atgctgaaaa tggcgcgtta ttcctttgcg atgggcaatg ctgcggaaaa cattaaacaa
    14341 atcgcccgtt acgctaccga tgataataat catgaaggcg cgctgaatgt gattcaggcg
    14401 gtgctggata acacatcccc ttttaacagc tgacctctct accggagccg tgcgctccgg
    14461 ttttcccttc tttttacatc ctgtacatca catcaaatca ttaaacttgc attaacttat
    14521 ttataactta tattgtcgca tgcaaatcag aatggtttcg aatgaaagtt aatgagaggt
    14581 ggttatggct tctactttta ccagcgacac attgcctgcc gatcacaaag cagctatccg
    14641 tcagatgaag cacgcgctgc gggcgcagct tggcgacgtc cagcagatct ttaatcagct
    14701 aagcgatgac attgccacgc gagtggctga aatcaacgca ctcaaagcac agggcgatgc
    14761 cgtctggccg gtgctgtctt atgccgatat caaagcaggt catgttactg cagagcagcg
    14821 cgaacagatt aaacgtcgcg gttgtgcggt gataaaaggc catttccccc gcgaacaagc
    14881 gctaggctgg gatcagtcga tgctggacta tctggaccgc aaccgctttg acgaggtcta
    14941 caaaggcccc ggcgataatt tcttcgggac gctcagcgct tcacgtcccg agatttaccc
    15001 catctactgg tcgcaggcgc aaatgcaggc ccgccagagt gaagaaatgg cgaatgcgca
    15061 gtcgtttctc aatcgtctgt ggacatttga aagtgatgga aagcaatggt ttaacccgga
    15121 tgtgagcgtc atctaccctg accgtatccg ccgccgtccg cccggaacga cctccaaagg
    15181 tcttggagcg cataccgact ccggggcgct ggaacgctgg ctgcttccag cgtatcagcg
    15241 cgttttcgcc aacgtcttta atggcaatct ggcgcaatat gatccctggc atgcggcaca
    15301 tcgtacggaa gttgaagagt acacggtgga caacaccacc aaatgttccg tgtttcggac
    15361 attccaaggc tggacagcgc tctctaatat gctgcctggt caggggctgc tgcacgttgt
    15421 gcccattcct gaagccatgg cgtacgtact gttacgtccg ctgcttgatg atgtgccgga
    15481 ggatgaactg tgcggcgtag cgcccggaag agtgttgccg gtatcagagc aatggcatcc
    15541 actgttaatt gaggcgttaa ccagcattcc aaaactcgag gccggagact ccgtctggtg
    15601 gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat caacagggtt ggggcaacgt
    15661 gatgtacatt cctgcggcac caatgtgcga gaaaaatctt gcctacgcgc acaaggtgaa
    15721 ggccgcactg gaaaaaggcg catcgccggg cgacttcccg cgcgaggact atgaaacaaa
    15781 ctgggaagga cgctttacgc ttgccgacct caacattcac ggtaagcgag cgttgggaat
    15841 cgacagctga gtgtcgtaca gcccttcccg ttccacggcg gtgttccctt ttcctgggcg
    15901 aacccgccgc actgactccc gcaccgccaa tcgtccgtgc agcagcagcg agcccaccgg
    15961 agcatccggg cgaatcagcc gctgttgcaa catttgcacc gcttcattgc ctagctcatc
    16021 gcgtggcaca tgcacagccg tcaggggaac atcctcgatt gccgcaaggt taaagccgtc
    16081 gatactcatc accgagacat cctgtggtac gcgcagacca cgacgatgta gcgcatttac
    16141 cgttcccgcc gccataaaat ccccgccgac cagaaacgcc gtcggcaacg tcttgccatt
    16201 ggcggcatcc agccacgctc cgaccttttc ttccgcttct ttagcgctaa aactgctgat
    16261 attcagcaaa tgacggtcat ggtgaaaggc cagattatgc tgacgccatg attctttaat
    16321 ccctgcaaac cgctgctcca tagtgtagcg gcgcaggcac atcacattca tcacctcccg
    16381 gtgacccatt tcaaacagat agcgggcggc gaacgcgcca attaaccgat gatccggcgc
    16441 aatcgccggg aggcgcattc gttcatcacg gcagttgatc agcacacaag gcttagcgag
    16501 atcggcggcc agatcatgga tgtgcggatc gtcaatcccc agcaggatcg ctgcctgcgt
    16561 atctgcttca ttcatccgcg agagaaagag cgcgggatcg ctgtcaaact cctccagcgc
    16621 gcagtaacgc agccgcactt catgcggcga taacgctttg ctgatgctct ggatcacacg
    16681 gtaataaaag atatcagatc gttggtcaaa agcgcgttgc ggcgcgaaaa taatcaggtt
    16741 gttcagcaac atacgacctg ctgccatgcc atccatcacg cccaactcac gcgcgcaggc
    16801 cagcacctgt tcgcgagcgc gtgcactggt attcgctttg ccagccagca cccgcgaaac
    16861 cgtactcggc gatagcccgg tacgtgcggc gatatcggcg attttcagct tcttgtccat
    16921 tttgtgatct gcctctaatt cggaaatgac agaattttca tgagcaaaaa agcccattct
    16981 gtctggcatt aaatccattt cagtaatgta tatcagtcac tcatggaaaa acgtgcacaa
    17041 aacccgcttt ctttcgcttt tagggtctct tactgtgaat tggtagaaca acttccatac
    17101 aaaaataatg gaaaaacgaa caataaagac tttatttatc aaaaagttaa aagtggtgta
    17161 acaactccat tctgacgttg ccgcacaaac cctataccat tctgtggagt gcatatgagt
    17221 cagggtatca ataacgatat gactgccagt aaaccgcgcc atatcgtcaa aaatctgcgt
    17281 tggtggatac tggtcctttt tttgctcggt gttactgtca actacatcac gcgtaactcg
    17341 ctgggtattc tcgcccccga actgaaaagc agcctaggga ttaccaccga acaatattcc
    17401 tggattgtag gtgctttcca gctcgcctat accctattcc aaccgttgtg cggatggctg
    17461 attgacgtca ttggcctcaa actgggcttt atgatctgcg cgggactgtg ggcactggcc
    17521 tgtctgatgc atgcgggggc gggaaactgg ggacatctgg cgctgttgcg cttctttatg
    17581 ggcggtgcgg aagccgcggc aaccccagca aacgccaaaa ccatcggtga atggtttcca
    17641 aaatcagaac gcccgatcgc cgcaggctgg gcaggcgtcg gtttttccat cggtgccatg
    17701 ctggccccac cgattatcta ttttgcccac gcttcattcg gctggcaggg tgcatttatg
    17761 ttcactggca tcctggcgat gctgtgggtg gtgctgtggt gggcgttcta tcacagcccg
    17821 gataagcatc ctaacctcag caaagccgag ctggacttta ttcgccagga taacgaagcg
    17881 cccccggtca agttaccctt tctgaccgca ctgaaaaccg tgggcaaaaa taaacgcttt
    17941 tacggtatcg ctatcccggc ctttatggca gaaccggcgt gggcggtgat gagtttctgg
    18001 gtgcctctat acctgtccaa aacctatggc atggacctga agcagatcgc attattcgcc
    18061 tggttgccgt tcctcgccgc tgatcttggc agtattgcca gcggctatct gaccaaactt
    18121 tacacccgcc tgtttgggct gagtcgtgtg aactccgttg tcgcatcatc ggtcagtggc
    18181 gcattcttga tggtttcgct ggcggtgatg gcctttacca ataacccgta tatcgccatc
    18241 gtccttatct ccatcggcgg cttcggtcac cagatcatct cctgcatgct gagcgcactg
    18301 gtggtggaat ccttcgacaa aggtcagatg gccactgtta acggcatgcg cggttctgcc
    18361 gcgtggatcg caagtttcct gttctccctg attatcgggg ttaccgctga caaaatcggc
    18421 tttaaccccc tgtttatcgc catgggcttc tttgacctga ttggcgcttt cttcctggtc
    18481 acctttattg ctgaacgtcg cgcaaaacgc gcctgagagt ggatgtaaac atgaaaacgc
    18541 tgaaaaactg gaccctgcac aaacagtctg cccaccacgt tgaactgctg gtcgatggcc
    18601 agcatatcct gtgcctgtac gtgctggaag aaaatctgtt ccgcgtgctg ttaaaacgca
    18661 aaggcgagct ggcgctagat cgcacctgga gcattgctcc ggcgcaggat gtgccatggg
    18721 aagggcgcag ccgcgaagat ctcagcggct ttagcctgcc cgcgtggaca gtgatccagc
    18781 acgacgacac cgtggttatc gccaccgagt cactgcgtgc aaccgttcac cagccgctgt
    18841 ggctggagtg gcactatcgt aacaacgaag gcgaatggct gccgttggtg aatgaccgcc
    18901 cgaccagcgc ttatctgctg aatgcccacg gcgatggcgt ggcacactat cagagccgtc
    18961 gtaaagatga acgcttttat ggcctcggag ataaagccgg cgatctgcaa cgcaacggta
    19021 aacgttacga gatgcgtaac ctcgacgcga tgggctacaa cgccgttagt actgacccgc
    19081 tgtacaaaca cattccattc accatcaccc gccgcgatga cattagcttc ggcctgtttt
    19141 atgacaacct cagcagctgc tggctggatc tgggcaacga gatcgacaac taccacaccg
    19201 cctatcgccg ctggcaggcg gaagcgggtg atatcgacta ctacctgttt accggcgaac
    19261 gcgtacttga tatcaccaaa gccttcgtac gcctgaccgg aaaaaccctg ttcggaccga
    19321 aatggagcct gggctacagc ggttcaacta tgcactacac cgatgcgccg gacgcacaaa
    19381 atcagttgat gaactttatc cgcctgtgtg aagaacatgc aattccctgc gattcgttcc
    19441 agctctcttc cggttatacc tcaattaacg gcaaacgcta tgtgttcaac tggaactacg
    19501 acaaagtgcc gcagccgaaa gtgatgagcc aggcatttca caatgctggt ttgcacctgg
    19561 ccgccaatat caagccgtgc ctgttgcagg atcacccacg ctataacgaa gtggcagaaa
    19621 gaggactgtt catccgtgat tcagaaacag atgaaccaga acgttccagc ttctgggatg
    19681 acgaaggttc taacctcgag tttactaatc cgcagaccat ccagtggtgg caggaaggcg
    19741 tgaccacaca gcttctggag atgggtatcg actccacctg gaacgacaac aacgaattcg
    19801 aagtatggga cggtgaagca cgctgtcacg gctttggtca gacgatcgcg atcaaacata
    19861 ttcgcccagt gatgccactg ctgatgatgc gagcctcgat ggaagcccag cagcgtttcg
    19921 ctccggaaaa acgtccgtac ttgatctccc gctccggctg cgcgggaatg cagcgctacg
    19981 tgcagacctg gagtggcgat aaccgcacta gctgggacac tcttcgctac aacacccgca
    20041 tgggtctggg gatgagcctc tccgggctgt ataacctcgg tcacgacgtc ggcggtttct
    20101 ccggtgataa gccggacccg gaactgtttg tacgctgggt acaaaacggc gtgatgcatc
    20161 cgcgctttac gattcactca tggaacgatg accataccgt aaacgaaccg tggatgtatc
    20221 cgggcgtcac tccggcgatc cgcagcgcta ttgagttgcg ctatcgcctg ctgccctact
    20281 tttataccct gatgtggcag gcatacgccg atgatgaacc aatgctacgt ccgactttcc
    20341 ttgaccacga gcacgacgtt cagacttttg aagagtgtga tgacttcctg cttggtcgcg
    20401 atattttggt tgccagcgtc gtagagcaag gcgagcgcca gcgccgtgta tggctgccag
    20461 ataacgaaac tggctggtac gatttctaca acggcgaatg gttcagtggc ggtcagtgga
    20521 tcaccctcga cgcaccgctg gagaaactgc cgctgatggt tcgtgccggg gctggtctac
    20581 cgctcagcaa acgcatcacc tatgtgagcg cagaacaaga cgatacccgc gaactgaaac
    20641 tgttcccgct gaaaggtgtg ggcacaacgt ccggcctgct gtttgaagac gatggcgaaa
    20701 gctggggcta ccaaactggt aatgcgcttt gggtggaatg ggaaatggtt tgcgatgggg
    20761 cgaccatcaa cctgaaagtc aacgcgcgcg gggattatcg tcctgcatgg agtgcactga
    20821 aagtgtcatt accattggaa gaaaaacgca cgctgctggt caatggtgtt gaagggagtg
    20881 aatggatgcg ttaaatcatt accggggccg ggaggcgact gcaccttacc cggtccagcc
    20941 atcgaatcct ttacctcagt atttcacgct gcaggaaggc agcaaacctg taaaccccgg
    21001 gagcttaaat aagtaagtga ccggggtgaa caggtgaagc caatgcatct gccgcatgaa
    21061 agacggcggg gaattaagcg tcgatccctt tactacgcag gtaatcttcg taattaccgc
    21121 taaagtcgat cgcgcgttcc ggggtgattt ccagaatgcg ggtcgccagg gagcttacga
    21181 actcacggtc gtgtgaaaca aagatcagcg tgccctgata cagttccagc gccatgttca
    21241 gtgactcgat ggattccata tccaggtggt tggtcggttc gtccatgatc agaatgttcg
    21301 gcttctgcat cattaactta ccaaacagca tccgcccttt ttccccaccg gaaagcactt
    21361 tagctggctt tttgatgtcg tcctggctga acagcaaacg accgagaatg ctgcgtaccg
    21421 cctgctcgtc atcgccttcc tgcttccact gactcatcca ttcgaacacg gtcagatcat
    21481 tttcaaactc atattcgtgg tcctgggcgt agtaaccaat gcgcgcgttt tcagaccatt
    21541 ttacggtgcc gctgtccggt tgcagatcgc ccaccagcgt tttcagcagc gttgatttac
    21601 cgacgccgtt ggtacccagt accgcaagtt tttcaccgac ctccagcagc aggttgagat
    21661 ttttaaacag tggaccgtta tcaaaccctt tggtcagacc ttccacttcc agcgcgttac
    21721 ggaacagttt cttatcctgt tcaaaacgga tgaacgggtt ctgacggctg gaggctttca
    21781 cctcttccag tttgatttta tcaatctggc gcgcgcgcga agttgcctgg cgagatttcg
    21841 aggcgttggc gctaaagcgg ctaacgaaag attgcaactc agcaatctgc gctttcttct
    21901 tggcgttatc ggccagcaga cgttcacgcg cctgggtcgc cgccgtcatg tactcatcgt
    21961 agttacccgg ataaacgcgc agctcgccgt aatccagatc cgccatgtgg gtacagacca
    22021 tgttaaggaa gtgacggtcg tgcgagatga tgatcatggt gctgtcacgc tcgttcagca
    22081 cctgttccag ccagcgaatg gtgtcgatgt cgaggttgtt ggtcggttcg tcgagcagga
    22141 gaatctccgg atcagcaaac agcgcctgcg ccagaagcac acgcagtttc cagccaggag
    22201 caacttcact catcgggccg tagtgctgtt ccactggaat tcccacgcca agcaacagtt
    22261 caccggcgcg agcttccgca gagtaaccgt ccatttcgcc gtatttaact tccagatcgg
    22321 ccactttata gccgtcttcc tcactcattt ccggcaaagc atagatgcgg tcacgctcct
    22381 gcttcacttc ccacaactct ttatgcccca tgatcaccgt atccagcaca gtgaactctt
    22441 caaaggcaaa ctgatcctga cgcagcttac caatgcgctc gttgggatcg agggaaacgt
    22501 tacccaaagt cggctctaag tcgccgccga ggatcttcat aaaggtggat ttaccactac
    22561 cgttcgcgcc aatcaggccg taacggttgc cgccgccgaa tttgacggaa atgttttcaa
    22621 acaacggctt actgccgaac tgcatggtga cgttactgga aactaacaca ggcgtatcct
    22681 gaaaagagat atgacaaacc gcgcattatg ccataagtct gaaagaagat cctgctcctc
    22741 gcttaaggtc tacactcaag ccaaaggcaa aaaatgtgat ttcgtacaca tctgatttca
    22801 ctgtgagctg gaatgaactt ataatgcgct tccaatactc taatattctc aacccaatgg
    22861 cctgccaggc acaaaatctc gcttaacatg aatatgaaat tgaaaacatt attcgcagcg
    22921 gccttcgctg ttgtcggctt ttgcagtacc gcctctgcgg taacttatcc tctgccaacc
    22981 gacgggagtc gcctggttgg tcagaatcag gtgatcacca ttcctgaagg taacactcag
    23041 ccgctggagt attttgccgc ggagtaccag atggggcttt ccaatatgat ggaagcgaac
    23101 ccgggtgtgg ataccttcct gccgaaaggc ggtactgtac tgaacattcc gcagcagctg
    23161 atcctgccgg ataccgttca tgaaggcatc gtcattaaca gtgcagagat gcgtctgtat
    23221 tactatccga aagggaccaa caccgttatc gtgctgccga tcggcattgg tcagttaggc
    23281 aaagatacgc ctatcaactg gaccaccaaa gttgagcgta agaaagcagg cccgacctgg
    23341 acgccgaccg ccaaaatgca cgcagagtac cgcgctgcgg gcgaaccgct tccggctgtc
    23401 gttccggcag gtccggataa cccgatgggg ctgtatgcac tctacatcgg tcgcctgtac
    23461 gctatccatg gcaccaacgc caacttcggt atcggcctgc gtgtaagtca tggttgtgtg
    23521 cgtctgcgta acgaagacat caaattcctg ttcgagaaag taccggtcgg tacccgcgta
    23581 cagtttattg atgagccggt aaaagcgacc accgagccag acggcagccg ttatattgaa
    23641 gtccataacc cgctgtctac caccgaagcc cagtttgaag gtcaggaaat tgtgccaatt
    23701 accctgacga agagcgtgca aacagtgacc ggtcagccag atgttgacca ggttgttctt
    23761 gatgaagcga ttaaaaaccg ctccgggatg ccggttcgtc tgaattaatc ttcaaaactt
    23821 aaagcaaaag gcggactcat aatccgcctt ttttatttgc cagaccctag ttggccggga
    23881 gtataactaa caaaacatat cccaccaacg ccgcccacaa cagcatcggt attgaataga
    23941 gatggaaacg ccaccagatg cggcgatcgt tcgccatacg tagcgcaatc aaatttgcca
    24001 gcgatccggg taataaccca aagccaccta cgtttaccgc ccataccagt aataaagacg
    24061 gcggcacata gttcagcaac aatatggtac tcggcacgtt actgatcacc tgcgataaac
    24121 cgattgccgt taaccataac ccgggttcag atagatgact cacgttaccc aacacgcctt
    24181 gcaacgctgg aagctgggtc agtaaatgga cgtcgataaa catcgccata aacaccagca
    24241 gcagcgtcca gtccacactg agcaccacgc gacgtgccag cagcgcaaag cctgccgcca
    24301 caatcaccag tccccacagc tcttgtttga actccagcgc cgtcagaaag acgatataca
    24361 gccccagaca actccacacc agccgcggtt tccactccgg tgtttgcacc cccgtatggt
    24421 attgcagcgc ctttccaggg aaacaacacc agcacaggag caggagcgtc agcatcattg
    24481 cgccagccag cggtgccatt tgggcaataa atccggcaaa cgaaagacca gaacgtcccc
    24541 agataagaat attttgcggg ttgccaattg gcgtcagtag cgaaccagcg ttgactgcca
    24601 gcgcctcaaa aataatcagc cgattaaccg ggatctcaca cagtcttttt agcgtgatag
    24661 tcagcggaac aacaataaac agcgcgacat cgttggtcag aaaggtagaa agcagcgccg
    24721 ccgccagcac cataaacatc gccagccgac gctccgtagc aaagcggcgc accattttgc
    24781 gccccagcac atcaaaataa ccgcttaact ccacaccttt ggtcagcagc atcaggccgc
    24841 ttaaggtgat gatggtgtgc cagtcgatag cagcaggcca ggatttcggt gcaaagggca
    24901 cgaaaaagct taatccgata ccaacaagaa ttaataactg aaaaaaacga tcgccttgca
    24961 gcgtgcgtaa aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca aacgaaaagc
    25021 gtcgagcgtc tcttcactaa catggtgctc catgccttcc gcgtcgcgac gggcgatttc
    25081 cggactgacg cccaacacca acaagaaatt ttcgactatc tgatgtcgct cgcggctttc
    25141 ctgcgccagc ttctctcctt ctgccgttaa aaacacgcca cgccagggga tcatttcaat
    25201 cagccccatg gttgccagcc gcttaagcat tttagccacc gtcggttgcg aaactcccag
    25261 acgagcagcc atgtccacct gacgagcttc ccccacttcc ctgatcaagt cagaaatcag
    25321 ctcaacgtaa tcatcaataa gctcgcgccg atgcgcctct ctgacctggc ggaacccttc
    25381 aacgtgctct tccacgttca ctaactgcgt cacttttttt gctgttggcg tacctgcgcg
    25441 acgactcatt gtgcttcctc attatggtga cgcatacata gcgttctcat ttcgtgcgca
    25501 cattgtaaac cagagttgcg aaggtacaaa aaattaacgt tttagcaata gctacataat
    25561 atagcctgtg ctatatctgt atgtaatgca atcatccctc aaggatcgac gggattagca
    25621 agtcaggagg tcttatgaat gagttcaaga agtgtatgcg cgtgtttagt cattctccct
    25681 ttaaagtacg gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac aaaccgcagc
    25741 aagataaacc ttccgataaa tagcggcgtc gcggtacgcc gcttcactcc tgcttttatg
    25801 caggcataac gcgttttggt ctgaaaaacc ccactttttg tcggatttgc aatccccttc
    25861 gcaaaagatt tgttcgtcag tagttgacct gaacggcggc tcgctctatc ttcttgcagc
    25921 cctacgtata ttgcggctcg cggatgcgga cccctttccc ctcttcacgc actcttgcag
    25981 gtattgaccc ttgacgccag ggtaagcaca tggcgtttgt tacgatagtg gcatatgaat
    26041 ttaaccctca aagaatcgct tgttacccgt agccgggtat ttagcccgtg gactgcgttc
    26101 tactttttac agtcgctatt aattaacctc ggcttaggtt accccttcag tttgctctac
    26161 accgctgcgt ttacggctat tttgcttttg ctatggcgaa cattgcctcg cgtacaaaaa
    26221 gttctggtcg gtgtcagttc gctggtggcg gcttgttatt tcccttttgc tcaggcctac
    26281 ggcgcgccta atttcaatac attgctggca ttgcactcca ccaatatgga agagtcgacc
    26341 gaaatcctga cgatttttcc gtggtacagc tacctggtcg gcttatttat ttttgcgctc
    26401 ggcgtaatag caatcaggcg aaaaaaagag aatgaaaaag cgcgctggaa taccttcgac
    26461 agcctgtgtc tggtattcag tgtggcgaca ttttttgttg ctcccgtgca aaacctggcc
    26521 tggggtggcg tatttaaact gaaagatact ggctatccgg tatttcgttt tgctaaggat
    26581 gtcatcgtca ataataacga ggtgattgaa gagcaagaac ggatggcaaa actttccgga
    26641 atgaaagata cctggacggt cactgccgtt aagccgaagt atcagaccta tgtggtggtg
    26701 atcggcgaaa gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg ggacaatacc
    26761 ccgtttgcca gcagcgttaa cggtttgata tttgctgact acattgccgc cagtggctcc
    26821 acgcagaaat cacttggctt aacgctcaat cgcgtagtcg atggcaaacc acagtttcag
    26881 gataactttg tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt ttccaaccag
    26941 ggccaaatcg gcgaatacga tactgctatc gccagcatcg ccaaacgtgc agatgaagtg
    27001 tacttcctga aagaaggtaa tttcgaagca gataaaaaca cgaaagacga agcgttactg
    27061 gatatgaccg ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat tgttctgcat
    27121 ctgatgggct cgcatccgca ggcctgcgac aggacacaag gcaaatacga aacctttgtg
    27181 caatcgaaag aaacgtcgtg ctatctctat accatgacgc aaacggacga tttactgcgc
    27241 aagctgtacg atcagttacg caacagcggc agcagcttct ctctggttta cttttctgac
    27301 cacggtctgg cctttaaaga gcgcggcaaa gacgtgcaat accttgccca tgatgataaa
    27361 tatcagcaaa atttccaggt gccttttatg gtcatttcca gcgacgataa agcgcatcgg
    27421 gtgattaaag cccgccgctc agccaatgac ttcttaggct ttttctcgca gtggacggga
    27481 attaaagcga aggaaataaa tatcaaatac ccgtttatat ctgagaagaa agccgggccg
    27541 atatacatca ccaacttcca gttacagaag gtggattaca accatctcgg aaccgatatt
    27601 ttcgacccga aaccttaaaa caaaaaatcc gccccgagag gcggattttt tatatcacca
    27661 aagtgattag aagcggtaac caacaccggc aatccaggtg cctacgtcaa cgctacgaat
    27721 acggctctgc tcgtaagaga agtccagagc aacgttttcc atcgggttga actgcagacc
    27781 agcaccgtag gagaaaccgt agtcgctggt gtcgtgtttg taggtcgggt attcagtggt
    27841 ctggaattta ccataaccca cccccactac accgtagatg cttgcccagt cgttaatgcg
    27901 gtaagccgga ccagcagtga tgccgtagta ctggtttttg ttgtagtcac cagagcttgc
    27961 agtacggctt ttctcggtgt aagtgaaaga accgatcaca cccagcgggc tgttgtcttc
    28021 ttcatagcgg tatttcaggt tgaaaccgcc cattttgttc atttggccct gagcgtcgct
    28081 ctgtgcgtaa ccgccagtta cggtagaagt cgcagctacg gaagtacctg cggtgaaagc
    28141 cagaactgcg gccagtgctg aaagacatgc aatttttttc ataaccacct caaatgtgat
    28201 tcaaataagt cctaagtttt aaatatatca aaaattaatg ggaaactctt cgcgatttgt
    28261 gatgtctaac gggccatttc atgtaacaga acgtttccat acaccgctat ccatctaaat
    28321 ttaaatcact ttttcagaga actgcgtaag tattacgcat gttttccctg tcattcatcc
    28381 agattattcc taatcaccag actaatgatt ccatcaatcc tggcgcattt tagtcaaaac
    28441 gggggaaaat tttttcaaca aatgctcaac cagcattggg tatatccagt acactccacg
    28501 ctttacttaa gtctagatat ttgtgggaga aaggatgcct ggttcattac gtaaaatgcc
    28561 ggtctggtta ccaatagtca tattgctcgt tgccatggcg tctattcagg gtggagcctc
    28621 gttagctaag tcactttttc ctctggtggg cgcaccgggt gtcactgcgc tgcgtctggc
    28681 attaggaacg ctgatcctca tcgcgttctt taagccatgg cgactgcgct ttgccaaaga
    28741 gcaacggtta ccgctgttgt tttacggcgt ttcgctgggt gggatgaatt atctttttta
    28801 tctttctatt cagacagtac cgctgggtat tgcggtggcg ctggagttca ccggaccact
    28861 ggcggtggcg ctgttctctt ctcgtcgccc ggtagatttc gtctgggttg tgctggcggt
    28921 tcttggtctg tggttcctgc taccgctggg gcaagacgtt tcccatgtcg atttaaccgg
    28981 ctgtgcgctg gcactggggg ccggggcttg ttgggctatt tacattttaa gtgggcaacg
    29041 cgcaggagcg gaacatggcc ctgcgacggt ggcaattggt tcgttgattg cagcgttaat
    29101 tttcgtgcca attggagcgc ttcaggctgg tgaagcactc tggcactggt cggttattcc
    29161 attgggtctg gctgtcgcta ttctctcgac cgctctgcct tattcgctgg aaatgattgc
    29221 cctcacccgt ttgccaacac ggacatttgg tacgctgatg agcatggaac cggcgctggc
    29281 tgccgtttcc gggatgattt tcctcggaga aacactgaca cccatacagc tgctggcgct
    29341 cggcgctatc atcgccgctt caatggggtc tacgctgaca gtacgcaaag agagcaaaat
    29401 aaaagaatta gacattaatt aaatttacat ttctgcatgg ttatgcataa ccatgcagaa
    29461 tttctcgcta cttttcctct acaccgtctt tatatatcga attatgcaaa agcatattta
    29521 ttccgaaaat tcctggcgag cagataaata agaattgttc ttatcaatat atctaactca
    29581 ttgaatcttt attagttttg tttttcacgc ttgttaccac tattagtgtg ataggaacag
    29641 ccagaatagc ggaacacata gccggtgcta tacttagtct cgttaattac tgggacataa
    29701 catcaagagg atatgaaatt atgagtaccg ctaaattagt taaatcaaaa gcgaccaatc
    29761 tgctttatac ccgcaacgat gtctccgaca gcgagaaaaa agcaacagta gagttgctga
    29821 atcgccaggt tatccagttt attgatcttt ctttgattac caaacaagcg cactggaaca
    29881 tgcgcggcgc taacttcatt gccgtacatg aaatgctgga tggcttccgc accgcactga
    29941 tcgatcatct ggataccatg gcagaacgtg cagtgcagct gggcggtgta gctctgggga
    30001 ccactcaagt tatcaacagc aaaaccccgc tgaaaagtta cccgctggac atccacaacg
    30061 ttcaggatca cctgaaagaa ctggctgacc gttacgcaat cgtcgctaat gacgtacgca
    30121 aagcgattgg cgaagcgaaa gatgacgaca ccgcagatat cctgaccgcc gcgtctcgcg
    30181 acctggataa attcctgtgg tttatcgagt ctaacatcga ataaatccat cgctgatggt
    30241 gcagaacttt agtacccgat aaaagcggct tcctgacagg aggccgtttt gttttgcagc
    30301 ccacctcaac gcacttattt agtgcatcca tctgctatct ccagctgatt aagtaaattt
    30361 tttgtatcca catcatcaca caatcgttac ataaagattg ttttttcatc aggttttacg
    30421 ctaaataatc actgtgttga gtgcacaatt ttagcgcacc agattggtgc cccagaatgg
    30481 tgcatcttca gggtattgcc ctataaatcg tgcatcacgt ttttgccgca tctcgaaaaa
    30541 tcaaggagtt gcaaaactgg cacgattttt tcatatatgt gaatgtcacg caggggatcg
    30601 tcccgtggat agaaaaaagg aaatgctatg aagtctgtat taaaagtttc actggctgca
    30661 ctgaccctgg cttttgcggt ttcttctcat gccgcggata aaaaattagt tgtcgcgacg
    30721 gataccgcct tcgttccgtt tgaatttaaa cagggcgata aatatgtggg ctttgacgtt
    30781 gatctgtggg ctgccatcgc taaagagctg aagctggatt acgaactgaa gccgatggat
    30841 ttcagtggga tcattccggc actgcaaacc aaaaacgtcg atctggcgct ggcgggcatt
    30901 accatcaccg acgagcgtaa aaaagcgatc gatttctctg acggctacta caaaagcggc
    30961 ctgttagtga tggtgaaagc taacaataac gatgtgaaaa gcgtgaaaga tctcgacggg
    31021 aaagtggttg ctgttaagag cggtactggc tccgttgatt acgcgaaagc aaacatcaaa
    31081 actaaagatc tgcgtcagtt cccgaacatc gataacgcct atatggaact gggcaccaac
    31141 cgcgcagacg ccgttctgca cgatacgcca aacattctgt acttcatcaa aaccgccggt
    31201 aacggtcagt tcaaagcggt aggtgactct ctggaagcgc agcaatacgg tattgcgttc
    31261 ccgaaaggta gcgacgagct gcgtgacaaa gtcaacggcg cgttgaaaac cctgcgcgag
    31321 aacggaactt acaacgaaat ctacaaaaaa tggttcggta ctgaaccgaa ataataacgc
    31381 tacacctgta aagcgcactg gcagttccct ctcccctctg gggagaggat tagggtgagg
    31441 ggcgcaaacc cgctccgggg ccattaatta ccctgaattt gattatttac accacggtaa
    31501 caggaacaac atatgcagtt tgactggagt gccatctggc ctgccattcc gcttctgatt
    31561 gaaggtgcca aaatgaccct gtggatttcg gtcctcggtc tggctggcgg tctggtaatc
    31621 ggattgctgg caggttttgc acgcaccttc ggtggttgga tagccaacca cgtcgcgctg
    31681 gtctttattg aggtgatccg cggcacacct atcgtcgtcc aggtgatgtt tatttatttc
    31741 gccctgccga tggcgtttaa cgacttacgc atcgacccat ttactgcggc ggtggtcacc
    31801 atcatgatca actccggcgc gtacattgcg gaaatcacgc gtggtgcggt gctgtctatt
    31861 cacaaaggtt ttcgtgaagc aggactggcg ctcggtcttt cacgttggga aaccattcgc
    31921 tacgtcattt taccgctggc actgcgtcgt atgctgccgc cgctgggtaa ccagtggatc
    31981 atcagcatta aagacacctc gctgtttatt gtgatcggcg tggcggaact gacccgtcag
    32041 gggcaagaaa ttattgccgg taacttccgc gcccttgaga tctggagcgc cgtggcggtg
    32101 ttctatctga ttattaccct ggtgctaagc tttattttgc gtcgtctgga aagaaggatg
    32161 aaaatcctgt gattgaattt aaaaacgtct ccaagcactt tggcccaacc caggtgcttc
    32221 acaatatcga tttgaacatt gcccagggcg aagtcgtggt gattatcggg ccgtccggtt
    32281 ccggtaaatc gaccctgctg cgctgcatca acaaactgga agaaatcacc tccggcgatc
    32341 tgattgtcga tggcctgaag gttaacgatc cgaaagttga cgagcgcctg attcgccagg
    32401 aagcaggtat ggtgttccag cagttttacc tcttcccgca tctgacggcg ctggaaaacg
    32461 tcatgtttgg tccgctacgc gtgcgtggcg cgaacaaaga agaggcggaa aaactggcgc
    32521 gtgagctgct ggcgaaagtc ggtctggcag aacgtgcaca tcactaccct tccgaacttt
    32581 ctggtggtca acagcagcgt gtggcgattg cccgcgcgct ggcggtgaag ccgaaaatga
    32641 tgctgtttga tgaaccgact tccgctcttg acccggaact gcgccatgaa gtgctgaagg
    32701 ttatgcagga tctggctgaa gaagggatga cgatggtgat cgtgacccac gaaatcggtt
    32761 ttgccgagaa agtagcttcg cggctgatct ttatcgacaa aggccggatt gcggaagatg
    32821 gcgatccgca ggtattgatc aagaacccgc cgagccagcg cttgcaggaa tttttgcagc
    32881 acgtctctta acttctctgc ctccctttca cccgaaaggg aggcacacca gattcctctc
    32941 atttaaaatc gcccctcccc cagcatctat acttatcttt ttgctctgtt ttctcactgg
    33001 aggagtcatg cggtggatcc tgttcatcct cttctgcctg ctgggcgcac ctgcccacgc
    33061 ggtatccata cccggcgtta caaccacaac gacaacggac tcaacgactg aaccggcccc
    33121 ggaaccggat atcgaacaaa aaaaagcggc ctatggcgca ctggcggatg tgctggataa
    33181 cgacacctcg cgtaaagaat tgatcgacca gttgcgtact gtcgccgcga cgccccctgc
    33241 ggaaccggtc ccgaagatcg tgccgccgac gctggttgaa gagcaaaccg tgctgcaaaa
    33301 ggtcaccgaa gtcagccgcc attatggtga agccctttcc gcccgcttcg ggcaacttta
    33361 tcgcaatatc accggctccc cgcataagcc atttaatcca caaaccttca gcaatgcgct
    33421 gagccatttt tcaatgttag cggtattagt gtttggtttt tactggctga ttcgcctgtg
    33481 cgcactgccg ctgtatcgca aaatgggtca gtgggcgagg caaaaaaatc gtgagcgcag
    33541 caactggttg cagcttccgg cgatgattat cggggcgttt attatcgacc tgctgttact
    33601 ggccctgaca ttatttgtcg gccaggtatt aagcgacaac ctgaatgcag gcagtcgcac
    33661 catcgctttc caacaaagtt tgtttctcaa cgcctttgcc ctgattgaat ttttcaaagc
    33721 cgtactacgc ctgatttttt gcccaaacgt ggcggagctg cgcccgttca cgattcagga
    33781 cgagagcgcc cgttactgga gtcgtcgcct gagctggtta agcagtttga taggttatgg
    33841 cctgattgtg gccgtgccga ttatctctaa tcaggtgaat gtacagatag gtgcgctggc
    33901 gaacgtcatc attatgctgt gcatgaccgt ctgggcgttg tacctgatct ttcgtaataa
    33961 aaaagagatt acccagcatt tgctcaactt tgcggagcat tcgctggcct ttttcagcct
    34021 gtttatccgc gcctttgcgc tggtgtggca ctggctggca agcgcctatt ttatcgtgct
    34081 gtttttcttt tcgttgttcg atccgggcaa cagcctgaaa tttatgatgg gtgcaacggt
    34141 gcgcagcctg gcgattattg gtatcgcagc gtttgtttcc ggtatgtttt cccgctggct
    34201 ggcaaaaacc atcactctct cgccacatac tcagcgtaac tatccagagt tgcaaaaacg
    34261 gctgaatggc tggctttcgg cggcgctgaa aacggcgcgt attctgaccg tctgcgtggc
    34321 ggtaatgttg ctgttgagcg catggggatt gttcgatttc tggaactggc tgcaaaacgg
    34381 cgcggggcag aaaaccgtag atatcctgat ccgtatcgca cttattcttt ttttctcggc
    34441 ggttggctgg acggtgctcg ccagtttgat cgaaaaccgg ctggcttcgg atattcatgg
    34501 ccgcccgcta cccagcgccc gtacgcgtac cctgctgacg ctgttccgta acgcgctggc
    34561 ggtgattatc agtaccatca ccatcatgat tgtgttgtcg gaaatcggcg tcaatatcgc
    34621 gccattgctg gcaggtgccg gggcattagg tctggctatc tcgtttggtt cgcaaacgct
    34681 ggtgaaagat atcattaccg gggtgtttat tcagtttgaa aacggcatga acactggaga
    34741 tttggtgact atcgggccgt tgaccggcac agtggaacgg atgtcgattc gctccgtggg
    34801 cgtgcgccag gataccgggg cgtatcacat cattccgtgg tcttcgataa ccacctttgc
    34861 taacttcgtc cgcggtattg gttcggtagt ggcaaactac gatgttgatc gccatgaaga
    34921 tgctgataaa gccaatcagg cactgaaaga tgcggtagcg gaattaatgg aaaacgaaga
    34981 aattcgcggg ctgattattg gtgaaccgaa ttttgccggg attgtcggct taagcaatac
    35041 cgcgtttaca ctgcgtgttt cgttcaccac gctgccactc aaacagtgga cggtacgctt
    35101 tgccctcgac agccaggtga aaaaacattt cgacctggcg ggcgttcgcg cgccagtgca
    35161 gacttatcag gtgctgccta ctccgggcgc gaccccggct gaaccgttac cgccggggga
    35221 gccaacgctt taacgctggc gattgacaaa acggcggcgc tgctcgtcgt tcataaaggt
    35281 ccaggcaata aagcgactct gcttttgccc ctgggccatc tcttttttta ccaccttcac
    35341 cgcgcccacg tcggtcagag cacgatacaa cggcggtaag ttttcaccac gagataccag
    35401 cgaggtaaac cacatcacct gcttcgcgaa gcctttgctc tcttcaatca tctttttgat
    35461 aaaggcgact tcaccacctt cacaccacaa ctcctgttgc tgaccaccaa agttcagtgc
    35521 atcgtctttg ttcagcccca ggttacggcg tttacgctca ctacctgccc gcgctgcagc
    35581 ggcggaatcg tggaatggcg ggttacacaa ggtcgcgtcg tattgctcgt ttttatggat
    35641 gatgccgtta aaaattgccc cactctcttt ttgccgacgc agacgaatgg cgcggttaag
    35701 ccccggatta gcactgataa tcgcctgcgc actgcttaac gcctggctgc tggtttcact
    35761 accggtaaaa cgccagccat attcatgtac gccaatcagc ggataaatac agttcgcacc
    35821 aacgccgata tccagaatgc tggcattcgc tggaattgtt ccgcttgctt cagccagtaa
    35881 atcggcaagg tgatgaatat aatccgcccg gcccggtact ggtgggcaga gaaaaccgtc
    35941 ggggatatcc cagttcgcta cggcgtaaaa atgggccagc aacgccttat tgagcgcctt
    36001 caccgccagc ggattggcaa agtctacgct ttgctccccg gcgggtgtaa gcgtgaggaa
    36061 ttgcctgagt tcaggattga cctgacaaag cgtggcgaga tcgtagcggc tgtgatgacg
    36121 gttgcgcgga tgcaaccccg gtttctgggc ggacatagca ttctccttta agcagcggcg
    36181 taagataccc gcataacgcg acagggtaaa tatctggctg cgtttatcat tccccaaaac
    36241 cttaacatgg ctatggttta tggcaattca tcataaaaat cagcattatg catgtcatta
    36301 atttgtctta ttttgtaaaa atatatcgtt caaaaaataa gcacatctct gtcaattttt
    36361 tgcgtcattt gcatcgcgca ggcgacctac acttatcagg cactacccgt tgtatttatg
    36421 aggatgtcat tatgaaaaag tgcctcacac tactgattgc caccgtcctg agcggaatct
    36481 ctctcacggc ttatgccgcg caaccgatga gtaacctgga cagcggtcag ctacggcccg
    36541 ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga tctggaggat aaactggcag
    36601 aaaaagcgcg cgaacaaggc gcgaaaggtt atgtcattaa ttccgcaggc ggaaatgacc
    36661 agatgttcgg tactgcaacc atctacaaat aaccccacca acgtaactgc attcgcctct
    36721 ggatgcagtt ttcgcctgtt ttgtaatcaa aaaacatcaa atatgaactc aatgtaaata
    36781 aatgtatttc tttttcgcgc aatgggtgat agaaaatcgc gccaagtgat aatgcttatc
    36841 aaaattatta tcacttgcat gaacactatc acgggattaa cagtggcatc gcatccgcag
    36901 agatgctttc tcgtggcagt gaaaatttca acatataaga aaaagtcacc tgcaaaatgg
    36961 aaaacaatcg caatttccct gccagacaat ttcattcgct cacgttcttt gccggtcttt
    37021 gtattggcat cacgcctgtg gctcaggcac tcgccgccga agggcaaact aacgcggatg
    37081 acacgctggt tgtcgaagca tcaacgcctt cgctttatgc gccacaacaa tctgccgatc
    37141 cgaaattctc gcgtccggta gcggatacta cccgcacgat gacggtgatt tctgaacaag
    37201 tgattaaaga tcagggcgca accaacctta ccgacgcgct caaaaacgcc cccggcg
//
LOCUS       DAETQN010000030        36331 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.030, whole genome shotgun sequence.
ACCESSION   DAETQN010000030 DAETQN010000000
VERSION     DAETQN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.030"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            417..1865
                     /gene="uacT"
                     /locus_tag="K0W68_001509"
     CDS             417..1865
                     /gene="uacT"
                     /locus_tag="K0W68_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBI3073108.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1867..1992
                     /locus_tag="K0W68_001510"
     CDS             1867..1992
                     /locus_tag="K0W68_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073109.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1989..2060)
                     /locus_tag="K0W68_001511"
     CDS             complement(1989..2060)
                     /locus_tag="K0W68_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073110.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            2115..2663
                     /gene="idi"
                     /locus_tag="K0W68_001512"
     CDS             2115..2663
                     /gene="idi"
                     /locus_tag="K0W68_001512"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBI3073111.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="K0W68_001513"
     CDS             complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="K0W68_001513"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBI3073112.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4233..5331)
                     /gene="prfB"
                     /locus_tag="K0W68_001514"
     CDS             complement(join(4233..5255,5257..5331))
                     /gene="prfB"
                     /locus_tag="K0W68_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBI3073113.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="K0W68_001515"
     CDS             complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="K0W68_001515"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBI3073114.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="K0W68_001516"
     CDS             complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="K0W68_001516"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBI3073115.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="K0W68_001517"
     CDS             complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="K0W68_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBI3073116.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8904..9425
                     /gene="fldB"
                     /locus_tag="K0W68_001518"
     CDS             8904..9425
                     /gene="fldB"
                     /locus_tag="K0W68_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBI3073117.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9465..9872)
                     /locus_tag="K0W68_001519"
     CDS             complement(9465..9872)
                     /locus_tag="K0W68_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HBI3073118.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="K0W68_001520"
     CDS             complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="K0W68_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBI3073119.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10362..11342
                     /gene="ygfZ"
                     /locus_tag="K0W68_001521"
     CDS             10362..11342
                     /gene="ygfZ"
                     /locus_tag="K0W68_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBI3073120.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11538..12197)
                     /locus_tag="K0W68_001522"
     CDS             complement(11538..12197)
                     /locus_tag="K0W68_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBI3073121.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12361..12672)
                     /locus_tag="K0W68_001523"
     CDS             complement(12361..12672)
                     /locus_tag="K0W68_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBI3073122.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            12717..14150
                     /gene="bglA"
                     /locus_tag="K0W68_001524"
     CDS             12717..14150
                     /gene="bglA"
                     /locus_tag="K0W68_001524"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBI3073123.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            complement(14207..14950)
                     /locus_tag="K0W68_001525"
     CDS             complement(14207..14950)
                     /locus_tag="K0W68_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI3073124.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="K0W68_001526"
     CDS             complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="K0W68_001526"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBI3073125.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="K0W68_001527"
     CDS             complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="K0W68_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBI3073126.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="K0W68_001528"
     CDS             complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="K0W68_001528"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBI3073127.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            20005..20088
                     /locus_tag="K0W68_001529"
     CDS             20005..20088
                     /locus_tag="K0W68_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073128.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="K0W68_001530"
                     /gene_synonym="visC"
     CDS             complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="K0W68_001530"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBI3073129.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="K0W68_001531"
                     /gene_synonym="visB"
     CDS             complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="K0W68_001531"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBI3073130.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="K0W68_001532"
     CDS             complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="K0W68_001532"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBI3073131.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="K0W68_001533"
     CDS             complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="K0W68_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBI3073132.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24659..24988
                     /gene="zapA"
                     /locus_tag="K0W68_001534"
     CDS             24659..24988
                     /gene="zapA"
                     /locus_tag="K0W68_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBI3073133.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25030..25213
                     /gene="ssrS"
                     /locus_tag="K0W68_001535"
     ncRNA           25030..25213
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="K0W68_001535"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25234..25836
                     /locus_tag="K0W68_001536"
     CDS             25234..25836
                     /locus_tag="K0W68_001536"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBI3073134.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25937..25996)
                     /locus_tag="K0W68_001537"
     CDS             complement(25937..25996)
                     /locus_tag="K0W68_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBI3073135.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="K0W68_001538"
     CDS             complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="K0W68_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBI3073136.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26616..27848)
                     /gene="serA"
                     /locus_tag="K0W68_001539"
     CDS             complement(26616..27848)
                     /gene="serA"
                     /locus_tag="K0W68_001539"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBI3073137.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="K0W68_001540"
     CDS             complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="K0W68_001540"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBI3073138.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29193..30086
                     /gene="argP"
                     /locus_tag="K0W68_001541"
     CDS             29193..30086
                     /gene="argP"
                     /locus_tag="K0W68_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBI3073139.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            30289..32433
                     /gene="scpA"
                     /locus_tag="K0W68_001542"
     CDS             30289..32433
                     /gene="scpA"
                     /locus_tag="K0W68_001542"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBI3073140.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            32426..33421
                     /gene="meaB"
                     /locus_tag="K0W68_001543"
     CDS             32426..33421
                     /gene="meaB"
                     /locus_tag="K0W68_001543"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBI3073141.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            33432..34217
                     /gene="scpB"
                     /locus_tag="K0W68_001544"
     CDS             33432..34217
                     /gene="scpB"
                     /locus_tag="K0W68_001544"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBI3073142.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            34241..35719
                     /gene="scpC"
                     /locus_tag="K0W68_001545"
     CDS             34241..35719
                     /gene="scpC"
                     /locus_tag="K0W68_001545"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HBI3073143.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(35716..>36331)
                     /locus_tag="K0W68_001546"
     CDS             complement(35716..>36331)
                     /locus_tag="K0W68_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3073144.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         8703 a         9742 c         9170 g         8716 t
ORIGIN      
        1 tgagttatgt cttctcaaat ttcgcgatgc aataaacggg catatattag gtagcatgac
       61 cgttttaatt ataattttgc gactgagttc aaattattgc ccctcaatct atgccgcttt
      121 cattatttca acattgccga tcaaaagagc gctatccaga taaaaacgat taaatgcgag
      181 agtgcgacat gccagaatga ttaatgaaat atcaatatga gaaaattaca gataattcca
      241 ttgtcaggtt ctcttgtcat tttcgtggtt taagcacgat tatgtgattg cctccgcaat
      301 tcctgtctct aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca
      361 gtttcattaa cgtaaacggt tgctttttac tctggcgggc gaaaggagaa acactgatga
      421 gcgccataga ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg
      481 accgcatatt atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt
      541 acgcaggtgc agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagatg
      601 ctattgcgat gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat
      661 gtatcggtat cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg
      721 ctgctgtaac accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat
      781 ttggtgccac tatcgccgcg ggttttatca ccacattatt agcgccattt attggtcgct
      841 tgatgccttt attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca
      901 tcattcaggt gggtattgac tgggccgccg gaggtaaagg gaatccgcaa tatggtaatc
      961 ccgtttattt aggtatctcc tttgctgtct taatttttat cttgctcatt actcgctatg
     1021 cgaaaggatt tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt
     1081 cgtggatgat gaatgaagtc aatttatccg ggctacatga tgcttcatgg tttgcgattg
     1141 tcacgccgat gtcatttggt atgccgattt tcgatcccgt ttccattctg accatgactg
     1201 ccgtgttaat catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag
     1261 tcggtcgtaa actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga
     1321 caatgatagg cggaacgttt aacagcttcc tccacacgtc attttcacaa aacgttggcc
     1381 tggttagcgt gacgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa
     1441 tcctgtttgg catggtgcca aaaatggcgg tgctggtcgc ctccattccg caatttgtgc
     1501 tgggcggtgc tgggctggtg atgttcggca tggtactggc gacagggatt cgaattctgt
     1561 cgcgctgtaa ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg
     1621 ttggcatgac tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac
     1681 cgttgctgca tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aatgtcttct
     1741 ttaatggcta ccagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa
     1801 aagtcaggac agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag
     1861 aataatatga attttttaat gcgcgttata ttcagtctgc tgttgctttt tactctctct
     1921 attcctgtca tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg
     1981 ctactttttt aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa
     2041 acgctcactt ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt
     2101 acatgtgaga aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca
     2161 cgggtacgct ggaaaagtat gccgcacaca cggcagacac ccgcttacat ctcgcgttct
     2221 ccagttggct gtttaatgcc aaaggacaat tattagttac ccgccgcgca ctgagcaaaa
     2281 aagcgtggcc tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca
     2341 acgaagacgc ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg
     2401 aatctatcta tcctgacttt cgctatcgcg ccaccgatcc gaatggcatt gtggaaaatg
     2461 aagtgtgtcc ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag
     2521 tgatggatta tcaatggtgt gatttagcag atgtattaca cggtattgat gccacaccgt
     2581 gggcgttcag tccgtggatg gtgatgcagg cagccaatag tgaagcaaga aaattgttgt
     2641 ctgctttcgc gcagcacaat taaaaaaccc cgacatttgc cggggttgtg agcataacgt
     2701 aatgcttatt ttaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg
     2761 ttggtgaaca gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag
     2821 ccatgttcca gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg
     2881 tctttcgcgg caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg
     2941 ctaaagccgt taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc
     3001 gggttaatgt cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag
     3061 gtcggctgaa tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc
     3121 agaccccagc tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg
     3181 aagttatcca gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg
     3241 gtcagttttt cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc
     3301 ttaccgagaa tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct
     3361 ttgtaatctg cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg
     3421 gaaatacctt cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc
     3481 accagacgct tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg
     3541 ttatggtggg tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc
     3601 gtttcaactt ccataaagcc gcggttcacc atgaactggc gaataccaga gaggatctgc
     3661 gagcgtactt taaaggtgtt acgggattca tcgttagaga tgagatccag ataacgctga
     3721 cgatagcgcg cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct
     3781 ttggtcagca gacgcagctc ggtacagtgg atagacagtt cgccggtttt ggttttgaac
     3841 agtttacctt tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa
     3901 acgccttccg gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc
     3961 agggtaacga aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg
     4021 acttcgatgt tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat
     4081 tggtcagagg tatgatcgcg acggaaatcg ttcgggaagg caatcccctg ctcgcgcagg
     4141 ttcgccagct tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg
     4201 ccctgtgcgt gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg
     4261 ataaattgat ccaggctgcc gtccagcacg gcctgcgtgt tgcgagtttc taccccggta
     4321 cgcagatctt taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg
     4381 atgtcagatt tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt
     4441 tcataaagct tcgctttcat ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg
     4501 tcgttctggc actgggtcac gatcccggtc gggatgtggg taatacgcac cgcagattcg
     4561 gtacggttaa cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc
     4621 gccgggttga tttcgatatc aatatcatca tcaacttccg gataaacaaa cgcagagctg
     4681 aacgacgtgt ggcgacgacc gccggagtca aacgggctct tacgcaccag gcggtgaacg
     4741 ccagtttctg tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat
     4801 ttaatacccg ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat
     4861 tctgcccagc gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg
     4921 ccagaccccg cctgaatatc gaggtagcag tcggcgctgt catattcgcc agagaacata
     4981 cggcggaact caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg
     5041 ttaaaggttt cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc
     5101 agcccctgtt tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta
     5161 cccagcgcct gtgcgcgttc gggttcattc cagacatccg gctgttccag ctcggcgttt
     5221 acttcttcca gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga
     5281 gcgttccgtg aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt
     5341 tcttttattg agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc
     5401 ttttttatag agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct
     5461 gcggttgccg cgaaactcgt tgatatcgag cttgtaagcc agttgcactt cgcgcacgcc
     5521 gttatccggc cagagggcgg tatcgacatt aaaagcaata ccatccagca gcggaccgcc
     5581 gccgaccggt tcgaccatga ctttcaaatg acgttcgccc accagccgct gttgcagcag
     5641 ccggaaatga ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg
     5701 cagcagctgc gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac
     5761 ttcgccttgc aatagcgaag ggtccagcca ctcagtaacc agttcgccaa accgttgttg
     5821 aaagagttcg aatttatccg cttccagcga caaacccgcc gccatcgcat gaccgccaaa
     5881 cttcagcatc atgccagggt agagtgtgtc taatcgctcc agcgcatcac gcatatgcag
     5941 cccctgaatg gagcgaccag aacctttcag cgtaccatca cccgcgggcg caaaggcgat
     6001 aaccggacgg tgaaaacgct ctttaatacg ggaagccaga ataccgacaa ccccctgatg
     6061 ccattcgggg tgatacattg ccagcccgcc gggtagcgtg tcacggctgc gctccagttt
     6121 ctcgcacagg gtcagggctt caacctgcat tccttgttcg atctcttttc gcgtctggtt
     6181 tagcgcatcg agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaagag
     6241 cgccacaccg acggacatat cgtccagtcg tccggcagca ttgagacgtg gccccagcgc
     6301 aaaacctaaa tcgctggcgg cgagtttttg tgcatcacgg tttgccactt caagcagcgc
     6361 tttaatcccc ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat
     6421 gcgattatta gcgtccagcg gtacgacgtc cgccactgtc cccagcgcta ccagatccag
     6481 cagttctgcc aggttaggaa ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa
     6541 aaaggtgcgc agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga
     6601 cgggaaatta cagtcgcgca agttagggtt aatgatcgct tccgccgcgg gtaatgtttc
     6661 gcccggcaaa tggtgatcgg taacaataac cggaatgccc aacgagcgag cgtgttcaac
     6721 ccccgcatgg gaggaaatac cgttatccac cgtgacaatt aactgcgcac cacgggcatg
     6781 ggcctgatcg accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggaaccag
     6841 gtagtcgata ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt
     6901 ggcaccgtcg gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaggcgtt
     6961 gtaaaggatc tcaacggcct tttcgacgcc gctcagttgc tgccagggca gcatcccttt
     7021 aacactgcgt tccagttctt gcgcactgcg cacgccccgg ctggcgtata aacggcgcag
     7081 caagggaggc aattcagcgg gcaagtctgc cgtttcatcg actgcacggc gacgaagttg
     7141 tatctgttgt ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat
     7201 tctttcatct ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact
     7261 gccggagtac cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg
     7321 tcgcagctgg ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta
     7381 tctttcgcac accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc
     7441 gggaaagcaa gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc
     7501 agtttgtggc agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc
     7561 agcgctttat aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta
     7621 ttggtgacat tgaccggagc cgtgccgcta acgtcataca ttggcccctg aatgatatgt
     7681 ttaccatcat cggtgatgta caacacgccg ctgttagtca gaactgtctt catgccagct
     7741 acgggcgcgg gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt
     7801 gccgcgtcat cagcctgagc aaaacctgaa aacgccgcta acaaagtaaa caacataaaa
     7861 cctttcttca taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga
     7921 tgaagttgcc gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg
     7981 ctgtggccca gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg
     8041 gcaaaagcgt gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcacg
     8101 gcataatgtt taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg
     8161 ggaaacaaca cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag
     8221 gtttccagcc agtaaaccgc ctcttcacct aacggtacca ggcgctcttt gttgccttta
     8281 ccaatgaccc gcactacgcc ctgacgcagg ctgatatcac tcattgtcag cccgaccagt
     8341 tcagagacac gcagcccggt agcatacaac acttcaagca tggctttatc gcgtagctcc
     8401 agtggctgat caattaatgg cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct
     8461 tttggcaaac gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga
     8521 aacttttcgc gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag
     8581 ctggtggctt tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt
     8641 tgcgccgtcg ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg
     8701 cgacggtaag cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga
     8761 aactgctcga tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat
     8821 ccattatgcc ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac
     8881 agcagaatcg agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt
     8941 acaccgaaat ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac
     9001 ataacctcaa ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta
     9061 tcccgacctg ggattttggt gaaatccagg aagactggga agccgtctgg gatcagctcg
     9121 acgacctgaa ccttgaaggt aaaattgttg cgctgtatgg gcttggcgat caactgggat
     9181 acggcgagtg gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg
     9241 tgaagttcgt cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga
     9301 ttgctgacgg gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta
     9361 gcgacgagcg tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg
     9421 cctgatctca ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct
     9481 tgttgcaaca aaatccgccg taaatcccgc cattcggctt cgtccatgct gtcggctgcc
     9541 agccataaat gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta
     9601 atcatccacg gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc
     9661 atcaacaagc gaatttcccc ctggcgagca ttaatacgcc gctggctgcg aacgcaatca
     9721 aacaccacca gcgaaagtaa caccatccat aacggggtgt aactgagtgg ccagggcatg
     9781 agtaaaataa cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag
     9841 gagacgcgca aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg
     9901 gaccatcatt tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag
     9961 gtccggatcg tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct
    10021 gtcgtactca tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg
    10081 gcatgcccaa tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa
    10141 tccagtaccc cgctattgta acgtgttttt cgccttcttt tacgggaata tcagtaaaca
    10201 ccatcgcgat cacgaaatta attcacaaca attcaatggc ttcatttttt tggaagtcgc
    10261 ctcgcagaag gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc
    10321 attagtcatt aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc
    10381 ctccccgtca gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact
    10441 gggcgcttgc caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag
    10501 cagatgtcag ccagatggca gaagatcagc acctgctcgc cgcccattgc gacgccaaag
    10561 gcaaaatgtg gagcaattta cgtctgttcc gcgacggcga tggctttgcg tggattgaac
    10621 ggcgcagcgt gcgtgaaccg cagctgactg aactgaaaaa atatgcggtg ttctctaaag
    10681 tgaccatcgc gccagacgac gagcatgtgc tgcttggtgt tgccggtttt caggcgcgcg
    10741 ccgcgctggc aaatatcttt agtgaactgc cttcgaaaga aaaacaggta gttaaagaag
    10801 gcgcgaccac tttgctatgg tttgaatacc cggcagaacg tttcctgatc gtaaccgatg
    10861 aagctactgc taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc
    10921 aacagtggct ggcattaaac attgaagcgg gtttcccggt gattgatgcc gccaacagcg
    10981 ggcagtttat cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag
    11041 gctgttatac cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aataaacgtg
    11101 cgctctggtt gctggcaggt agcgccagcc gactgccgga agctggtgaa gacttagagc
    11161 tgaaaatggg cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag
    11221 atggtcaggt cgtggtacag gtcgtcatga ataacgatat ggaaccggat agcatcttcc
    11281 gcgtacgcga cgatgcgaat acattgcata ttgagccgct gccgtattcg ctcgaagagt
    11341 aaatctcttt atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg
    11401 tctgggattt gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc
    11461 acgccgaatc cggcagttgt gctccgatgc ctgatgcgac gctgacgcgt cttttcaggc
    11521 ctacaaaggc atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac
    11581 acactgccgc cgagcacgaa gccgtgccag atggcatgat tgtagggaat gcgtttgcag
    11641 acatagaaaa tcactccgag cgaataaacc acgccgccta ccgccaataa ggtaacgctg
    11701 cccgccgcga gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc
    11761 agataggtca ccagagataa aattttgaat cggtgcgcga tggtcagttt aaacagaata
    11821 cccagcaatg ccaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc
    11881 cccaccagca aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca
    11941 aatttcttca gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg
    12001 aggaacagca ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg
    12061 gcattgagat ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccaac
    12121 ccaatgccgt gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg
    12181 aggggcttct gaaccataac ttactccgga gaaacgtaca cgcacatgta tacctctcca
    12241 gcgtaactga gaatagttcc agtgaacacc tgttagctaa aataaattct gatttgagac
    12301 taatctccta aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat
    12361 ttaaagacat ttaaattcaa tcacataaaa ctgtgtctga ccgggataga tgtcggcaat
    12421 gacctttatc agttcagtca gggtcatatt ttcctgctct gcatgttttt ccgtcagcgt
    12481 atccagcgtt acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc
    12541 aaaacgcccg acacgaagca catcacccgt tttgaagtgc gattcagact cgtcgcggat
    12601 ggtgatggtt ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc
    12661 gtttggctgc atggtactat ttcctgtaag aattgactca tctggagcct atgatagtga
    12721 aaaaactcac cttacagaaa gatttcttat ggggcggcgc agttgccgct catcaggtcg
    12781 aaggcggctg gaacaaaggc ggcaaagggc cgagcatttg tgacgttctg accggtggcg
    12841 cacacggcgt gccgcgcgaa atcaccaaag aagtcttgcc aggcaaatac tatccaaacc
    12901 atgaagccgt tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg
    12961 gcttcaaatg ttttcggact tccatagcct ggacgcgcat ttttccaaaa ggcgatgaag
    13021 ctcagccaaa cgaagaaggg ctgaagttct acgatgatat gttcgatgaa ctgctgaaat
    13081 acaacatcga accggtgatc accctctccc actttgaaat gccgctgcat ctggtgcagc
    13141 aatacggtag ctggaccaac cgtaaagtgg ttgatttctt tgtccgtttc gcggaagtgg
    13201 tgtttgaacg ctataagcat aaagtcaaat actggatgac cttcaacgaa attaacaacc
    13261 agcgtaactg gcgtgcaccg ctgttcggtt actgctgctc cggcgtggtt tataccgagc
    13321 atgagaaccc ggaagagacg atgtatcagg tgctgcatca ccagtttgtc gccagcgccc
    13381 tggcggtgaa agctgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga
    13441 tggtgccgct ctatccctac tgctgtaacc cggacgatgt gatgttcgct caggagtcga
    13501 tgcgcgaacg ctacgtcttt accgatgtgc agctacgcgg ctattacccg tcctatgtgt
    13561 tgaacgagtg ggagcgtcgc ggatttaaca tcaaaatgga agacggcgat ctcgatgtac
    13621 tgcgcgaagg cacctgcgat tatcttggtt tcagctatta catgaccaac gcagtgaagg
    13681 ccgaaggcgg caccggcgat gcgatctctg gtttcgaagg cagcgtaccg aacccgtatg
    13741 ttaaagcgtc tgactggggc tggcagattg atccagtagg tctgcgctat gccctttgcg
    13801 aactgtatga gcgttatcag aggccgctgt ttattgtcga aaacggtttt ggcgcttacg
    13861 acaaagtgga agaagatggc agcatcaacg acgactaccg cattgactac ctgcgcgccc
    13921 atatcgaaga gatgaaaaaa gcggtgactt acgatggcgt ggatctgatg ggctacacac
    13981 cgtggggctg catcgactgc gtatcgttca ccaccgggca gtacagcaaa cgctacggct
    14041 ttatctatgt gaataaacat gacgacggta ctggcgatat gtcgcgttca cgtaagaaga
    14101 gctttaactg gtacaaagag gtgattacca gcaacggcga gaagctttaa gtcgatgaag
    14161 taccggatgc aatacttgtt gcatccggtc agacaacttc cctgttttat ttcccgcccg
    14221 ccaaatcgat aaaacttccc gtgacgtaag aggctttatc acttagtagc cagacaatgg
    14281 cctgcgcgac ctcttctgcc tgtccaccac gctgcatggg gatgttcgac ttaacgcgat
    14341 cgacgcgtcc aggctcgccg ccgctggcgt gcatttcggt aaaaataaac cctggccgca
    14401 cgcagttaac gcggaccccc tgcgcggcga cttccagcga tagtccggtg gttaacgtat
    14461 caatcgcccc tttcgatgcc gcgtaatcaa catattcccc tggcgaaccc aaccgtgagg
    14521 ccaccgaaaa gacattgacg atagcgccgc cactgccacc atttttaagc gccatgcgtt
    14581 ttaccgcctc gcggcagcag agaaaatatc ccgtcatgtt ggtggaaagt actcggttga
    14641 ttcgctctgc ggtaaggttt tcaacggtgc actgggtaaa caagatcccg gcgttattga
    14701 ccagcgctgc tagcggttca tcgtgctgat cgattgctgt aaacattgcg atgacctgat
    14761 tttcgtcgct gatatccgcc tggagcacga atgccttgcc accggcttgc gtgattaagt
    14821 tcaccacttc ctgcgccgca tggaggtttt gttgataatt aaccgccacc gtatactctt
    14881 ctttcgccaa cagtaatgca gttgcccgcc cgatgccgcg actgccatcg gtcacaagtg
    14941 ctatagccat ttgtttttcc gagaaaaatt aattcgttga cgtatctttg agattgctaa
    15001 caaactggct caatcttcaa tgttggcacg cttttcccct cgccctttca gggagagggc
    15061 cggggtgagg gtaaatattc gcgccagtgc cggcctgttc ccctcaccct aaccctctcc
    15121 ccaaaggggc gaggggactg tctgagcact tttgtacttt gtcatctgac taaaaaggcg
    15181 ccgaagcgcc tttagaaaat agtcgaatca gtgaattact ggtattcgct aatcggtacg
    15241 caggagcaga acaggttacg gtcgccgtaa acatcatcca gacgtttcac cgtcggccag
    15301 tatttgtctg ccacgcctgc cgggaatacc gcaacttcac ggctgtacgg atgcgcccac
    15361 tcggcgacca gttcgctctg aatgtgcggc gcgttcacca gcgggttatc ttccagcggc
    15421 cagacagcgg ctttcacctg gtcaatttct gcgcggatag ccagcatcgc gtcgataaag
    15481 cgatccagtt ccactttgct ttcagattca gtcggttcaa ccatcagcgt acccgccacc
    15541 gggaacgaca tcgtcggcgc gtggaaaccg tagtcgatca ggcgcttggc aatatccagc
    15601 tcgctgatgc cggtttcttc tttcagcggg cgaatatcga gaatacattc gtgcgccacg
    15661 cgaccgtcgc gaccggtata cagcaccggg aaggcatcct gcaggcggct ggcaatatag
    15721 ttggcgttga ggattgccac ctggcttgct tttttcagcc cttctgcgcc catcatgcgg
    15781 atgtacatcc agctgattgg caggatagag gcgctaccga acggtgccgc agaaactgcg
    15841 ccctgacggg ttaacatgcc ttcgatttgc accacgctat gacccggtac aaacggtgcc
    15901 agatgcgctt tcacaccgat cggtcccata cccggaccac caccgccgtg cggaatgcag
    15961 aaagttttat gcaggttgag gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg
    16021 ccaacctggg cgttcatgtt cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg
    16081 acttcacaca cttcacggat cgtttcttca tacacgccgt gggtagaagg ataagtcacc
    16141 atgatacagg agaggttatc gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg
    16201 atgttgccgt ttttatcaca cgccacaacc accacctgca ttcctgccat atgtgcagaa
    16261 gcggggttag ttccgtgcgc agaagccggg atcaggcaga tatcgcgatg cccttcgttg
    16321 cggctttcat gataatgacg aatcgccagc aggcccgcgt attcgccctg tgcgccggag
    16381 ttcggctgca tacaaacggc gtcgtaaccg gtcagtttca ccagccagtc agccagctgc
    16441 gcaatcatct gctgataacc ttcagcctgt tccggcgggc aaaacgggtg cagttcggca
    16501 aattccggcc aggtgattgg gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa
    16561 cccagcggga tcatcgcctg attcagcgcc agatctttac gctccagcga gtgcatatag
    16621 cgcatcattt cggtttcgct gtggtagcga ttaaacaccg gatgggtgag gatttcgtcg
    16681 tcgcgcagca tcgcaggctg gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg
    16741 tcgatctcca gcccgtggtt atcgcccagc agcacgctga aaagctgcat tacgttttca
    16801 cgcgtggtgg tttcatcaag ggtgatcccc acggcgttca ggatatcgct acgcaggttg
    16861 atttcagccg cttcggcacg cgtcagtacg cccgctttgt cggccacttc cacacacaag
    16921 gtgtcgaaat agtgcgcatg gcgcagtttc agaccttttt gttgcaggcc cgccgccaga
    16981 atatcggtca gacggtgaat gcggttagcg atacgtttca ggccaaccgg gccgtgataa
    17041 acggcataca ggctggcgat gtttgccagc agtacctggg aagtacaaat gttggagttc
    17101 gctttctcac gacgaatatg ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg
    17161 ccagctgcat ctttcgatac accgataata cggcccggca ttgagcgttt gtattcatct
    17221 ttcgccgcaa agaatgccgc gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc
    17281 gccgaaccaa aaacaatatc cgcgccctgt ttacccggcg cagttaacag caccagcgcc
    17341 ataatatcgg cggcaacgct gaccacaatt ttgcgtgatt tcagttcgct aataagcgca
    17401 gtgtagtcgt gaatttcacc ggtagtgcct acctgctgta acagcacgcc gaagacgtcc
    17461 tgatggtcga gcactttttg cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca
    17521 cgagtacgga ccacatccag cgtttgcgga tgcacatcgg aagccacgaa gaagcggttg
    17581 gcatttttca gtttgctgac gcgtttcgcc atcgccattg cttcggcggc agcggtggcc
    17641 tcgtccagaa gagaagcaga ggccatatcc agtccagtca aatccagcgt tacctgctgg
    17701 aagttgagca gtgcttcaag gcggccctgg gagacttcag gttgatacgg agtgtacgcg
    17761 gtataccagc ccggattttc cagcatgtta cgcaggataa ccggcggtag ctgcacggcg
    17821 gtgtaaccca tgccgatgta agacgtgaag cgtttattgc gactggcaat agccttgagt
    17881 tctgccagtg cggcgtattc ggtcgccggt gcgccaacct gcggcggggt cgcaagctga
    17941 atatctttcg gtacaatctg gccggtcagc gcgtttaacg attgtgcgcc aacggcattc
    18001 agcatttctt gctgttgcgc ggcgtccggt ccgatatggc gttcaataaa agcgccgctg
    18061 ttttcaagct ggcttaacgt ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt
    18121 gtgaacctct ctccttacga agagagtgag ggtgaggcat aaattttcct caccctgatc
    18181 ctctcccgca gaagaggaat aaagccgtta ctcgtcttct aacaatgctt cgtatgcggt
    18241 cgcatccagc agtgattcca gttcgctttc atcgctggct ttgattttaa agatccagcc
    18301 acctgcatac ggttcgctgt tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc
    18361 cacgatttca ccgcttactg gcgcataaat gtctgacgcc gcttttactg attcggcaac
    18421 cgcgcagtca tcgcccgcgc taaccgttgc gcccacttcc ggcaggtcaa caaacaccat
    18481 atcgcctaac agctcctgag catgttcggt aataccaacg gtgtaagtgc cgtcggcttc
    18541 tttacgcagc cattcgtgtt ctttgctgta tttcagttct gctggtacgt tgctcatcaa
    18601 tcaatctcca aaaaagtaaa tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt
    18661 gtcactttaa ccgacatttc acggttgcga atttgcacaa tcgccgtttc gccaatacct
    18721 tccagcacgc gcgccagcgc aatgctgtaa cccagcgtcg gggagaaagt accgctggtg
    18781 ataatgcctt catgctggtt gccctgcgca tcggtaaagc gtaccggcag ttcattacgc
    18841 agcacgcctt tttcggtcat caccagacca accagttttt ctgtaccatg ctcacgctgc
    18901 acttccaggg cttcacgacc gataaagtca cgatctgccg gttcccaggc gatagtccag
    18961 cccatgttgg cggctaaagg agaaatagtt tcgtccatct cctgaccata aagattcatg
    19021 cccgcttcca gacgcagcgt gtcacgcgcg cccaagccac atggcttaac acccgcttcc
    19081 accagcgcac gccagaaatc ggccgctttt tcattgggca gcgcaatttc atagcccgct
    19141 tcaccggtat aaccagtggt ggcaataaac agatcgcccg cctgcacgcc aaagaacggt
    19201 ttcatccctt ccaccgcctg acgctgggcg tcattaaaca gtgtggcagc ttttgcctgc
    19261 gcattcggcc cttgcacggc aatcatggaa aggtcatcac gaacggtaat ttcgatgccg
    19321 aaaggttcag cgtgttgggt aatccaggag aggtcttttt cgcgggtggc ggagttaaca
    19381 acgaggcgga agaaatcttc agtaaagtag tagacgatga ggtcatctat cacaccgcca
    19441 gaggcattca acatccccga gtaaagggct ttgccgcttt tggtgagctt cgccacatcg
    19501 ttcgccagca gataacgcag aaactcccgg gtgcggctgc cgcggagatc gacgatggtc
    19561 atatgtgaca catcaaacat tccggcatcg gtacgtaccg catgatgttc gtcgatttgc
    19621 gaaccgtaat gcagcggcat catccagccg tggaaatcca ccatgcgagc gccgcaaagc
    19681 gtgtgttgtt cgtacaaagg agtctgttgt gccatcttgt cctcattgaa taagcggggc
    19741 tgacaacttt tcatggtgaa attatcacca cgaaacccag catcggagcc actcccggtc
    19801 cccaacgcaa tcgttctctt ttgcctgaac ttaccaccga aacagactgt taaccataag
    19861 gtaaaattga tcatcacatt agcttatggt taaaaaacgc caaaatcgcg acagaataaa
    19921 aaaccaaaaa acacaccagt ttctatataa agatgatgcc attagaaagc taatttgaat
    19981 aaggcaatat taagagctaa aaaaatgtca aaaaacacta aatcaaaaaa taatgacatt
    20041 agaaaatata atgcgaaaac ggaggtgaaa ttagtttatt tcaaatgagg aaaatctccc
    20101 ggcgaaaaaa ccgggagatg aaagtgtgat gggtatcaaa taaacagcag aagagaaatt
    20161 tttaacgcag ccattcaggc aaatcgttta atcccattgc ctggcggata agttgtggtt
    20221 taacgccagg aagcgtgtcg gccagtttca aaccaatatc acgcagcagt tttttcgccg
    20281 gattggtacc ggaaaacaga tcgcggaatc cctgcatacc agccagcatc agcgccgcac
    20341 tgtgcttgcg gctacgctca taccggcgca ggtaaatgta ctgcccgatg tctttcccct
    20401 gacgatgcaa ccgtttcagt tcagcaatca gctctgcagc atccataaag ccaagattta
    20461 ctccctgccc cgccagcggg tgaatggtat gcgcggcgtc gcccaccagc accagacggt
    20521 gcgcggcaaa ctggcgcgca taacgccccg tcagtgggaa cacctgacgc gcgctctcaa
    20581 ccttgcataa gcccaggcga ttatcaaaag cgatatttaa cgcgcgatta aattcatctt
    20641 cacttgcctg ctgcatccgc tgcgcttcct ctggcgacag tgaccagaca attgagcaaa
    20701 gatgcggatc gctaagcggt aaaaaggcca gaatgccttc gccatggaaa acctgccgcg
    20761 ccaccgcatc atgcggttct tccgtgcgaa tggtcgctac cagcgcgtga tgctgataat
    20821 cccagaaagt cagcggaata tctgctttgt tgcgcaacca ggaattagca ccgtccgcgc
    20881 caatcaccag acgcgccgtt aacatgctgc catctttcag cgtcaggaaa gtttcatttt
    20941 ctccccaggc gacctgctgt aattctgcgg gggccaacag agtgatatct gacgactgct
    21001 gcgctttgtt ccacagcgcg tagtgaatca ctgagttttc aacgatatgc ccaagatggc
    21061 tatagcccat gctttgatcg tcaaacgaaa tgtgaccaaa actgtctttg tcccacactt
    21121 ccataccgtg ataacaactg gccctacgag agagaatgtc ctgccagacg ccaagacggg
    21181 tgagtaattt ttcgctggcg gcattgatag ccgaaacgcg cagttgtggt ggtgcatccg
    21241 ccgccagagg ttccggtacg cgctgctcca gtacggcaac gcgtaagccg ctcccctgta
    21301 agccgcaggc aaccgccagc cccaccatgc cgccaccaac aatggctaca tcaacacttt
    21361 gcattgttta ttccttaaaa ccgccttcaa cgcgccaccc aaccgagggt gcgctgcgcc
    21421 agcacatcgc gtgccggggt gaataattcc atcgtcatca gtccgatgtt gcgcccgaca
    21481 accagcggtg cccaacggtt ggcaaaaaga tgtaccaggc tgtccgtgac gccgatggtt
    21541 gcttcgcgat cgctctgtcg acgctgctga tagcggcaca atatgccgta atcaccgatg
    21601 tcttctccgc gctcatgcgc ctgagtcagg gtttccgcaa ggctcatgac atctcgcata
    21661 ccgaggttaa acccttgccc ggcaatcggg tgcagagttt gcgccgcatt gcccaccagc
    21721 acggtacgat gggtaatagc tctggcggcg cgggttaacg ccagcggata agcactgcgt
    21781 ttaccggcgt gggtaatttt tccaagtcgc cagccaaagg ccgactggag ttcacggcaa
    21841 aacttctcgt cgctccacga cagcacctct tcgcgccgtt ccagtggatg acaccagacc
    21901 agcgaacagc gtccgtcaga catcggcaac atcgccagcg ggccatgttg cgtgaagcgt
    21961 tcaaaagcgc ggccttcgtg cgcaacggaa gtcgcaacgt tggcaatcac ggccagttgt
    22021 tcgtaaggct cctgctgcca gtcaacgccg cacgcggtgg ctaacgctga atgggtgcca
    22081 tcagctgcta ccagcacgcg gcccgtcagc gtctcgccac tctccagcgt cacttcaacg
    22141 tgactctgag tacgggcaac gttagccacg cgatcagggc aatgcagcgt tacgccaggt
    22201 gctttacgca gcaatgcaaa cagccgttgc ccgacattgt gcaattcgac aacctgtccc
    22261 agcgccgcca gttggtaatc ttctgcggcg agggtgacaa atccagcgtg accacgatcg
    22321 ctgacatgca cggtagtgat ggcggttgcg taatccgcca gagattgcca gacgccgatg
    22381 cgcgccagtt gctgacaggt ccccgcagcc agcgcaatag ctcgtccatc aaagcccgga
    22441 tgagcatgtg actctggcgc ggtcgcttca atcaaatgta ccggcagcgc cccgtgactt
    22501 aaccgggaaa tagccagcgc cagcgtcgcg cccgccatgc cgccaccgac gatgattacg
    22561 ctcattgctt tctcgcagct gccatcaacg cttcgatttc ttccggcttt ttcaccacgc
    22621 tggcggtgag gttttcgttg ccggtttcgg taatcacaat gtcgtcttca atacgaatgc
    22681 cgataccgcg atattgttct ggcacatccg catccggcgc gatatacaga ccaggctcta
    22741 cggtcagtac catccccggt tccagaatgc gcgagcgatc ctgaccataa acgccaacgt
    22801 catggacatc cagtcctaac cagtggctaa ggccatgcat aaagaaagga cgatgggcgt
    22861 tctgagcgat cagttcatca acatcacctt tcaggatgcc gagttttacc aggccgctaa
    22921 ccatgatgcg caccacttca ccagtgactt ccagaatgga agttcccgga cgatacaggc
    22981 gcaggctggt ttcgagagac tccagcacaa tgtcgtagat ttcacgctgg gcctgggtga
    23041 atttgccgtt gaccgggaag gtgcgggtaa tatcgcccgc gtaacctttg tattcacaac
    23101 ccgcgtcaat caacaccagg tcaccgtcgc gcagttcact ctcgttttcg gtgtagtgca
    23161 gaatgcagcc gttttcaccg ctgccgacaa tggtgttata ggacggatag cgcgcaccgt
    23221 ggcggttaaa ttcgtggtga atttcgcctt ccagatggta ctcgaacatt cccggacggc
    23281 atttttccat cgcccgtgta tgtgccatgg cggtgatttc tcccgcgcgg cggagtacgg
    23341 caatctcttc tggcgatttg aacaggcgca tttcatgaac aacaggacgc cagtcgatca
    23401 tcgttgccgg tgcggtgaga ttttgccgcg aacctttacg cagtttttcc agcgcactgt
    23461 tcatgatttc atcagcatat gcatattcgc cctgggcatg gtaaaccaca tccaggccgt
    23521 taagtagttg ataaagttgc tgattgattt cgctgaatgc cagtgcgcgg tcaacgccca
    23581 gtttctctgg cgcggcatcc tggcctaagc gacggccaaa ccagatctcc gccgtcaggt
    23641 cgcgaacgcg gttaaacaga acgctgtggt tatgagtgtc atcgctttta atcagcacca
    23701 gcaccgcttc caattcgtta aagccggtga agtaccagaa gtcactgttc tgacgatagg
    23761 ggtattcgct gtcggcgcta cgtgttactt ctggtgcagc aaaaatcagc gcggcgctgc
    23821 cgggttgcat ttgctccacc agggcctgac ggcgacgctg aaactcttgc cgggatatct
    23881 cactcataac actctcctta cgttttttgt ttttagtgta gagtcggttt ttgtacttct
    23941 ggcgcggtcg gttgcggatg agtaaaggtg tcgtggcata acagcgcggc aacacgaacg
    24001 tattcgatga tctcttcaag cgacatttca agctcttcct gatcttcgtc ttcgtcgtaa
    24061 cccagttgcg caatgttacg cagatcgtcg atagcttcac cggtttcgcc ggtcacttta
    24121 tccagcttcg gttgcgtaac gccaagacca agcaggaagt gattgaccca acctgccaat
    24181 gcatcagccc gatcgaaaac gctgacatca tcgccatcag gcagataaag ctgaaaaagg
    24241 aagccgtcat cctgcagggc atcgctggtg gcagagtgca ttttacgcag tgcctgtgcc
    24301 agctcatgac cgaaagccat gccttcgttc gtcaggtcgt gaagtagcgg cagccatgag
    24361 ctgtcatcgt taccgccaca tatcatccca ctgattaaac catgcatctc ggcaggggtc
    24421 agccccgtcc cttgttggtt cagatactgg ttcatttcgt tgtaaccagg catttcgttc
    24481 tgtatagaca taagcattcg tcgtcaaagg gaggaatatt catgatatgc taccactttg
    24541 ggccctggtg gaccagaaaa gggcttgtct cttctcatca gggtagctat agtgtcgccc
    24601 cttcgcagac catgggtcta aagacgaagg cagcgcagtc aatcagcagg aaggtggcat
    24661 gtctgcacaa cccgtcgata tccaaatttt tggccgttca ctgcgtgtga actgcccgcc
    24721 tgaccaaagg gatgcgttga atcaggcagc ggacgatctg aaccaacggt tgcaagatct
    24781 gaaagaacgc actagagtca caaatactga acagttggtc ttcattgccg cattgaatat
    24841 cagctatgag ttagcgcaag aaaaagcgaa gactcgtgac tacgcggcaa gtatggaaca
    24901 gcgtattcgg atgctgcagc agaccataga acaagcgtta cttgaacaag gtcgcatcac
    24961 cgaaaaaact aaccaaaact ttgaatgaca cttttcggtt tactgtggta gagtaaccgt
    25021 gaagacaaaa tttctctgag atgttcgcaa gcgggccagt cccctgagcc gatatttcat
    25081 accacaagaa tgtggcgctc cgcggttggt gagcatgctc ggtccgtccg agaagcctta
    25141 aaactgcgac gacacattca ccttgaacca agggttcaag ggttacagcc tgcggcggca
    25201 tctcggagat tcccttctta tctggcacca gccatgacgc aactaccaga actcccactg
    25261 acattatccc gacaagaaat ccgcaaaatg attcggcaac gtcgtcgtgc gttaacgccg
    25321 gaacaacagc aggaaatggg tcaacaagcc gctacccgga tgatgactta tcccccggtg
    25381 gtgatggcgc atacggtcgc tgtattcctc tcttttgatg gtgaactcga cacccagccg
    25441 ctaatagaac aactctggcg cgccggtaag cgcgtatatc ttccagtttt gcatcccttt
    25501 agtgccggta atttgctgtt cctgaattac catccgcaaa gcgaactggt gatgaacagg
    25561 ttgaagatcc atgagccaaa actggatgtg cgtgacgtgc tacccctttc ccgattagac
    25621 gtgctgatca caccgctggt cgcctttgat gagtacggtc agcgcctggg aatgggcggt
    25681 ggtttttatg atcggacctt acaaaactgg cagcactata aaacgcaacc ggtgggttat
    25741 gcgcatgatt gtcagttggt ggaaaaactc cccgttgaag agtgggatat ccctcttcct
    25801 gcggtggtta caccgtcgaa agtctgggag tggtaagggc gatacacctg catcgccctg
    25861 attgacatcg ttgattcttt aacctaattt agtgagtaag ggtaagggag gattgctcct
    25921 cccctgtgac tgactgttaa taagctggga aacttatgag taacagtaca atcagtatga
    25981 tggcaagtcg catcataacc cttctccttc aagccctcgc ttcggtgagg gctttaccgt
    26041 tacagcccca tgctgcccag ctatcgtaag ctctctttaa ataaccacaa cgcattgatc
    26101 tacctttaaa tcttttcttc gagcgtgctc gaaaaaccca tgcaaaacag agataatttt
    26161 tgggaaaaag gatctattac ggcagtaaac gtgggcgatg aagtggctat caaaaagttc
    26221 ggttcagggc gatgcacccc gcatcgccct gattgacatc gttgattctt tgacctaatt
    26281 tagtgagtaa gggtaagaga ggatgactcc tcccctgaga ctgactgtta ataagctccg
    26341 aaacttatca gtaacagtac aatcagtatg atgacaagtc gcatcataac ccttctcctt
    26401 taagccctcg cttcggtgag ggctttaccg ttacagcccc atgctgtccc gccatcgtaa
    26461 atccccatta aataaacaca acgcattgat ctgagtttga tttattttct ggagcagact
    26521 cgcaaagtag aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt
    26581 cagtgacctg cccgttaatt ttcagagaag gggaattagt acagcagacg ggcgcgaatg
    26641 gtacccggaa tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcagcttca
    26701 atatcaataa ctacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg
    26761 acgccctgct cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg
    26821 atgtgcatca gacgacgacc accatgcagt ggcagcgaga cttccgggaa gttcaccgca
    26881 gagagcgttg agccattgtc agaatacttg atcaatttac ccgcaacttc caggccgata
    26941 ttctcctgcg cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat
    27001 tcacacagtg gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg
    27061 attgccgccc ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc
    27121 acagtaccgc gcgaggcatt aatcagcagc gagccgggct tcattagcga aatctctttc
    27181 gcgcccatca tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc
    27241 atattcagca ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt
    27301 tcaatatcat aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta
    27361 ccaatatgac cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc
    27421 gccagtttgt tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat
    27481 agcagcagca gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg
    27541 ttaaataccg ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttccgata
    27601 cagaaacagc caatagcgac cagtttttct gcggcgttga tcacgtcttc agtcagatgg
    27661 gtacgggatc gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca
    27721 tccagcgcgc ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc
    27781 gccttttggt gcacgccttc taccagcaga aacttaatct tgtctttctc cagcgatacc
    27841 tttgccattt acccaatcct gtcttttgaa atgttgtgtg cggatttgca tccgcctttc
    27901 aacatatcaa aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat
    27961 atcacgcaaa ggtatttctt ggttgaatat gctggtttag aggatttatt tagagcaatc
    28021 gacaatagcc aagtaaaaac gtgacacatg tcaccaaatt taatgaagag aattttttta
    28081 acgggggagg ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca
    28141 atcagcgcaa cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc
    28201 gcatttatgg cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg
    28261 atcacgttgc cgttatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc
    28321 accagctggc gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat
    28381 ttgcccagaa tatcaacctg cttggaagcg tcagcaatac agataaattt ttccgcaacc
    28441 gaagcaatga ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg
    28501 ccgttgattt catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca
    28561 aaaacgtgaa tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc
    28621 ccttcaatct ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct
    28681 gtacctacac caacaatggt gccgggctga acatactgaa gtgccgccca tcctactgct
    28741 tttttcaatt catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt
    28801 atatacaaaa aaagcgattc agaccccgtt ggcaagccgc gtggttaact catccataaa
    28861 atatcgcgca atggcgggca tcccctttcg ccccgcaaat aaagcataca acggtctggg
    28921 tatgccgctc cacggtgcga acaggcgcac cagttccccc cgttcgcaag cccctgttta
    28981 caggcaaatt gaggcaataa cgccacccca ttcaacacaa ccagggcacg aactcgctga
    29041 gcgaaatgca ttagcgcaaa tccctgatct tttaagctgc gcgtttaacc acggatacaa
    29101 atgttacccg ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag
    29161 gaaaaagcat ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac
    29221 aggcactgga tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca
    29281 ttacacaatc agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc
    29341 tgttggtgcg taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc
    29401 tgcgccaggt ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc
    29461 cgctgctgct ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttccggcac
    29521 tggctcctgt gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc
    29581 gtactcagga acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg
    29641 cgctgccgag ttgtcttgtc gataaacttg gtgcgctcga ctatctgttc gtcagctcaa
    29701 aaccctttgc cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcac
    29761 ctgtggtcgc ctttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg
    29821 atctgcctcc aggcagcgtg ccctgccata tcgttaattc ttcagaagcg ttcgtgcaac
    29881 ttgctcgcca gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagaactag
    29941 ccagcggtga actgattgac ttaacgccag ggctatttca acgccggatg ctctactggc
    30001 accgctttgc tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgttg ctcgattatg
    30061 gtcacaaagt ccttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc
    30121 gttatcctca cttctttttg tattccttga atcacatcac aaaatagaca aatctcaggc
    30181 ggcaaaaaac gacgtctgaa tgcatttttt tgctggcgac aaacccacgt aaaaagctca
    30241 ccgtaagcgc aaataccctc attttgattg cgttttacgg agcaaataat gtctaacgag
    30301 caggagtggc aacagcttgc caacaaggaa ttgagccgtc gggagaaaac tgtcgactcg
    30361 ctggttcagc aaaccgcgga agggatcgcc atcaagcagc tgtataccga agccgatctc
    30421 gataatctgg aggtgacagg tacccttcct ggtttgccgc cctacgttcg tggcccgcgt
    30481 gccactatgt ataccgccca accgtggacc atccgtcagt atgctggttt ttcaacagca
    30541 aaagagtcca acgcttttta tcgccgtaac ctggccgccg ggcaaaaagg tctttccgtt
    30601 gcgtttgacc ttgccaccca ccgtggctac gactccgata acccacgcgt ggcgggtgac
    30661 gtcggcaaag cgggcgtcgc tatcgacacc gtggaagata tgaaagtcct gttcgaccag
    30721 atcccgctgg ataaaatgtc ggtttcgatg accatgaatg gcgcagtgct accagtactg
    30781 gcgttttata tcgtcgccgc agaagagcaa ggtgttacac ctgataaact gaccggcacc
    30841 attcaaaacg atattctcaa agagtacctc tgccgcaaca cctatattta cccaccaaaa
    30901 ccgtcaatgc gcattatcgc cgacatcatc gcctggtgtt ccggcaacat gccgcgattt
    30961 aataccatca gtatcagcgg ttaccacatg ggggaagcgg gtgccaactg cgtgcagcag
    31021 gtagcattta cgctcgccga tgggattgag tacatcaaag cagcaatctc tgccggactg
    31081 aaaattgatg acttcgctcc tcgcctgtcg ttcttcttcg gtatcggcat ggatctgttt
    31141 atgaacgtcg ccatgttgcg tgcggcacgt tatttatgga gcgaagcggt cagtggattt
    31201 ggcgcacagg atccgaaatc actggcgctg cgtacccact gccagacctc aggctggagc
    31261 ctgactgaac aggatccgta taacaacgtt atccgcacca ccattgaagc actggctgcg
    31321 acgctgggcg gtactcagtc actgcatacc aacgcctttg acgaagcgct tggtttgcct
    31381 accgatttct cagcacgcat tgcccgcaac acccagatca tcatccagga agaatcagaa
    31441 ctctgccgca ccgtcgatcc actggccgga tcctattaca ttgagtcgct gaccgatcaa
    31501 atcgtcaaac aagccagagc tattatccaa cagatcgacg aagccggtgg catggcgaaa
    31561 gcgatcgaag caggtctgcc aaaacgaatg atcgaagagg cctcagcgcg cgaacagtcg
    31621 ctgatcgacc aaggcaagcg tgtcatcgtt ggtgtcaaca agtacaaact ggatcacgaa
    31681 gacgaaaccg atgtacttga gatcgacaac gtgatggtgc gtaacgagca aattgcttcg
    31741 ctggaacgca ttcgcgccac ccgtgatgat gccgccgtaa ccgccgcgtt gaacgccctg
    31801 actcacgccg cacagcataa cgaaaacctg ctggctgccg ctgttaatgc cgctcgcgtt
    31861 cgcgccaccc tgggtgaaat ttccgatgcg ctggaagtcg ctttcgaccg ttatctggtg
    31921 ccaagccagt gtgttaccgg cgtgattgcg caaagctatc atcagtctga gaaatcggcc
    31981 tccgagttcg atgccattgt tgcgcaaacg gagcagttcc ttgccgacaa tggtcgtcgc
    32041 ccgcgcattc tgatcgctaa gatgggccag gatggacacg atcgcggcgc gaaagtgatc
    32101 gccagcgcct attccgatct cggtttcgac gtagatttaa gcccgatgtt ctctacacct
    32161 gaagagatcg cccgcctggc cgtagaaaac gacgttcacg tagtgggcgc atcctcactg
    32221 gctgccgatc ataaaacgct gatcccggaa ctggtcgaag cgctgaaaaa atggggacgc
    32281 gaagatatct gcgtggtcgc gggtggcgtc attccgccgc aggattacgc cttcctgcaa
    32341 gagcgcggcg tggcggcgat ttatggtcca ggtacaccta tgctcgacag tgtgcgcgac
    32401 gtactgaatc tgataagcca gcatcatgat taatgaagcc acgctggcag aaagtattcg
    32461 ccgcttacgt cagggtgagc gtgccacgct cgcccaggcc atgacgctgg tggaaagccg
    32521 tcacccacgt catcaggcgc taagtacaca gctgcttgac gccattatgc cgttctgtgg
    32581 taacgccctg cgactgggcg ttaccggcac acccggcgcg gggaaaagta cctttcttga
    32641 agcctttggc atgttgttga ttcgtgaggg attaaaggtt gctgttattg cggtcgatcc
    32701 cagcagcccg gtcactggcg gtagcattct cggggataaa acccgcatga atgacctggc
    32761 gcgtgccgag gcggcgttta ttcgcccggt accctcctcc ggtcatctgg gcggtgccag
    32821 tcagcgagca cgggaattaa tgctgttatg cgaagcggcg ggttatgacg tagtgattgt
    32881 cgaaacggtt ggcgtcgggc agtcggaaac ggaagtcgcc cgcatggtgg actgttttat
    32941 ctcgttgcaa attgccggtg gcggcgatga tctgcagggc attaaaaaag ggctgatgga
    33001 agtggctgat ctgctcgtta tcaacaaaga cgatggcgat aaccatacca atgtcgccat
    33061 tgcccggcat atgtacgaga gtgccctgca tattctgcga cgtaaatacg acgaatggca
    33121 gccacgggtt ctgacttgta gcgcactgga aaaacgtgga atcgatgaga tctggcacgc
    33181 catcatcgac ttcaaaaccg cgctaactgc cagtggtcgt ttacaacaag tgcggcaaca
    33241 acaatcggtg gaatggctgc gtaagcagac cgaagaagaa gtactgaatc acctgttcgc
    33301 gaatgaagat ttcgatcgct attaccgcca gacgctttta gcggtcaaaa acaatacgct
    33361 ctcaccgcgc accggcctgc ggcagctcag tgaatttatc cagacgcaat attttgatta
    33421 aaggaatttt tatgtcttat cagtatgtga acgttgccac tatcaacaaa gtggcggtca
    33481 ttgagtttaa ctatggacga aaacttaatg ccttaagtaa agtctttatt gatgatctta
    33541 tgcaggcgtt aagcgatctc aaccggccgg aaattcgctg tatcattttg cgggcgccaa
    33601 gtggatccaa agtcttctcc gcaggtcacg atattcacga actgccgtct ggcggtcgcg
    33661 atccgctctc ctatgatgat ccattgcgtc aaatcacccg catgatccaa aaattcccga
    33721 aaccgatcat ttcgatggtg gaaggtagtg tttggggtgg cgcatttgaa atgatcatga
    33781 gttccgatct gatcatcgcc gccagtacct caaccttctc aatgatgcct gtaaacctcg
    33841 gcgtcccgta taacctggtc ggcattcaca acctgacccg cgacgcgggc ttccacattg
    33901 tcaaagagct gatttttacc gcttcgccaa tcaccgccca gcgcgcgttg gctgtcggca
    33961 tcctcaacca tgttgtggaa gtggaagaac tggaagattt caccttacaa atggcgcacc
    34021 acatctctga gaaagcgccg ttagccattg ctgttatcaa agaagagctg cgtgtactgg
    34081 gcgaagcgca caccatgaac tccgatgaat ttgaacgtat tcaggggatg cgccgcgcgg
    34141 tttatgacag cgaagattac caggaaggga tgaacgcttt cctcgaaaaa cgtaaaccta
    34201 atttcgttgg tcattaatcc ctgcgaacga aggagtaaaa atggaaactc agtggacaag
    34261 gatgaccgcc gatgaagcgg cagaaattat ccagcataac gacatggtgg catttagcgg
    34321 ctttacccca gcgggttcgc cgaaagcctt accgaccgcg attgcccgca gagctaacga
    34381 acagcatgag gccaaaaagc cgtatcaaat tcgcctgctg acgggggcgt caatcagcgc
    34441 cgccgctgac gatgtacttt ctgacgccga tgctgtttcc tggcgtgcgc catatcaaac
    34501 atcgtccggt ttacgtaaaa agatcaatca gggcgcggtg agtttcgttg acctgcattt
    34561 gagcgaagtg gcgcaaatgg tcaattacgg tttcttcggc gacattgatg ttgccgtcat
    34621 cgaagcatcg gcactggcac cggatggtcg agtctggtta accagcggga tcggtaatgc
    34681 gccgacatgg ctgctgcggg cgaagaaagt gatcattgaa cttaatcact atcacgatcc
    34741 gcgcgttgca gaactggcgg atattgtgat tcctggcgca ccaccgcggc gcaatagcgt
    34801 gtcgatcttc catgcaatgg atcgcgtcgg tacccgctat gtgcaaatcg atccgaaaaa
    34861 gattgtcgcc gtcgtggaaa ccaacttgcc cgacgccggt aatatgctgg ataagcaaaa
    34921 tcccatgtgc cagcagattg ccgataacgt ggtcacgttc ttattgcagg aaatggcgca
    34981 tgggcgtatt ccgccggaat ttctgccgct gcaaagtggc gtgggcaata tcaataatgc
    35041 ggtaatggcg cgtctggggg aaaacccgga aattcctccg ttcatgatgt attcggaagt
    35101 gctacaggaa tcggtggtgc atttactgga aaccggcaaa atcagcgggg ccagcgcctc
    35161 cagcctgaca atctcggccg attccctgcg caagatttac gacaatatgg attactttgc
    35221 cagccgcatt gtgttgcgtc cgcaggagat ttccaataac ccggaaatca tccgtcgtct
    35281 gggcgtcatc gctctgaacg tcggcctgga gtttgatatt tacgggcatg caaattcaac
    35341 gcacgtagcc ggggtcgatc tgatgaacgg catcggcggc agcggtgatt ttgaacgcaa
    35401 cgcgtatctg tcgatcttta tggctccgtc gattgctaaa gaaggcaaga tctcaaccgt
    35461 cgtgccaatg tgcagccatg ttgatcacag cgaacacagc gtcaaagtga tcatcaccga
    35521 acaagggatc gccgatctgc gcggtctttc cccgcttcaa cgcgcccgca ctatcattga
    35581 taattgtgca catcctatgt atcgggatta tctgcatcgc tatctggaaa atgcgcccgg
    35641 cggacatatt caccacgatc ttagccacgt cttcgactta caccgtaatt taattgcaac
    35701 cggctcgatg ctgggttaat ggtttgtccc gtcacgatct tcagcaatat gctgaagcat
    35761 cgtggcggta taacgatgat ttctgattga ataaagatat tcctgcatat acatcggcct
    35821 gtgcggcgca tcaaaatatt tcagcgttgc cggattcacc agtcgccagg caaaatgcgg
    35881 gatcaccgtc agaaaatgtc cgcgctccac ggcgctgatt ttggccataa aactgtaggg
    35941 acgataaata atggtgggat tgatcccaca aggtcgcata ttcgcatcaa tcattgcttc
    36001 gaagttagcc cggttttgaa agcgcatttg tagccagggc agctcctgaa gcagctcttg
    36061 ctcttgacgc tcttcataac ggcgagaaac gagaaagccc aatcgcagcg gtggtaattc
    36121 actgatagtc aggttttcca gttcctgaac tcgtgcagat acatgctgtg gggagataat
    36181 aaaatctaac tgacggtcaa aaagattatc gataacgccg ttttcgctga actcaactgg
    36241 ctgggcggtc acgccttcat acttatctcc gagactaatc aactgatcga aaataattgt
    36301 cggataagta ttatctatgc caatcacaat t
//
LOCUS       DAETQN010000031        35778 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.031, whole genome shotgun sequence.
ACCESSION   DAETQN010000031 DAETQN010000000
VERSION     DAETQN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35778
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.031"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..581)
                     /locus_tag="K0W68_001547"
                     /pseudo
     CDS             complement(1..581)
                     /locus_tag="K0W68_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(810..1730)
                     /gene="malM"
                     /locus_tag="K0W68_001548"
     CDS             complement(810..1730)
                     /gene="malM"
                     /locus_tag="K0W68_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBI3073145.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVSYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="K0W68_001549"
     CDS             complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="K0W68_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBI3073146.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3294..4409)
                     /gene="malK"
                     /locus_tag="K0W68_001550"
     CDS             complement(3294..4409)
                     /gene="malK"
                     /locus_tag="K0W68_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBI3073147.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4774..5964
                     /gene="malE"
                     /locus_tag="K0W68_001551"
     CDS             4774..5964
                     /gene="malE"
                     /locus_tag="K0W68_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBI3073148.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6118..7662
                     /gene="malF"
                     /locus_tag="K0W68_001552"
     CDS             6118..7662
                     /gene="malF"
                     /locus_tag="K0W68_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBI3073149.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7677..8567
                     /gene="malG"
                     /locus_tag="K0W68_001553"
     CDS             7677..8567
                     /gene="malG"
                     /locus_tag="K0W68_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBI3073150.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8939..10414
                     /gene="xylE"
                     /locus_tag="K0W68_001554"
     CDS             8939..10414
                     /gene="xylE"
                     /locus_tag="K0W68_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HBI3073151.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="K0W68_001555"
     CDS             complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="K0W68_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBI3073152.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10994..11137)
                     /locus_tag="K0W68_001556"
     CDS             complement(10994..11137)
                     /locus_tag="K0W68_001556"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073153.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            11138..11416
                     /locus_tag="K0W68_001557"
     CDS             11138..11416
                     /locus_tag="K0W68_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073154.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQSDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11463..13559)
                     /locus_tag="K0W68_001558"
     CDS             complement(11463..13559)
                     /locus_tag="K0W68_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBI3073155.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13559..14296)
                     /locus_tag="K0W68_001559"
     CDS             complement(13559..14296)
                     /locus_tag="K0W68_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBI3073156.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14293..14931)
                     /locus_tag="K0W68_001560"
     CDS             complement(14293..14931)
                     /locus_tag="K0W68_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBI3073157.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15045..15287)
                     /locus_tag="K0W68_001561"
     CDS             complement(15045..15287)
                     /locus_tag="K0W68_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073158.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="K0W68_001562"
     CDS             complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="K0W68_001562"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBI3073159.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17923..19272
                     /gene="lysC"
                     /locus_tag="K0W68_001563"
     CDS             17923..19272
                     /gene="lysC"
                     /locus_tag="K0W68_001563"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBI3073160.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19524..19796
                     /locus_tag="K0W68_001564"
     CDS             19524..19796
                     /locus_tag="K0W68_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBI3073161.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="K0W68_001565"
     CDS             complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="K0W68_001565"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBI3073162.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21149..21986
                     /locus_tag="K0W68_001566"
                     /pseudo
     CDS             21149..21986
                     /locus_tag="K0W68_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            22056..22862
                     /locus_tag="K0W68_001567"
     CDS             22056..22862
                     /locus_tag="K0W68_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI3073163.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22872..23279
                     /locus_tag="K0W68_001568"
     CDS             22872..23279
                     /locus_tag="K0W68_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HBI3073164.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            23279..23773
                     /locus_tag="K0W68_001569"
                     /pseudo
     CDS             23279..23773
                     /locus_tag="K0W68_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23839..24636
                     /locus_tag="K0W68_001570"
     CDS             23839..24636
                     /locus_tag="K0W68_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBI3073165.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24647..25471
                     /locus_tag="K0W68_001571"
     CDS             24647..25471
                     /locus_tag="K0W68_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBI3073166.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25523..26751
                     /gene="sorE"
                     /locus_tag="K0W68_001572"
                     /pseudo
     CDS             25523..26751
                     /gene="sorE"
                     /locus_tag="K0W68_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            27134..27824
                     /gene="pepE"
                     /locus_tag="K0W68_001573"
                     /pseudo
     CDS             27134..27824
                     /gene="pepE"
                     /locus_tag="K0W68_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
     gene            complement(27915..29546)
                     /locus_tag="K0W68_001574"
     CDS             complement(27915..29546)
                     /locus_tag="K0W68_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBI3073167.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29766..33449)
                     /gene="metH"
                     /locus_tag="K0W68_001575"
     CDS             complement(29766..33449)
                     /gene="metH"
                     /locus_tag="K0W68_001575"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBI3073168.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33649..34473
                     /gene="iclR"
                     /locus_tag="K0W68_001576"
     CDS             33649..34473
                     /gene="iclR"
                     /locus_tag="K0W68_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBI3073169.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34790..35778
                     /gene="espL4"
                     /locus_tag="K0W68_001577"
                     /pseudo
     CDS             34790..35778
                     /gene="espL4"
                     /locus_tag="K0W68_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT         8598 a         9231 c         9102 g         8847 t
ORIGIN      
        1 actatgacca agttgactct gcgagaagtc aactgtatca agaatatttt ggctaaaatc
       61 aacacattcg ccactcgttt tgagaaaaat cacattgtgt aaacgtcgac atttaaatat
      121 gacattatca actgttgcct tagtaaaatt gcaattctca atttctttac attcgaaagt
      181 tacatgttgt agatttgatg ataaaaaaga caccttagaa ataacgtcac ctttaaaatg
      241 acaatttatt aaatcaaaat cagacaggtc gatatacccc tgcgcatctt tatccatcag
      301 aaaattacaa gcgcattcta ttttttcatt tctgtagaat tttacactta tagtatcatt
      361 actaattgag acttcacgta acatatttat tgcatgattt tttcgttccg ttgctttata
      421 tttaaaaata tatcactgat ataaataaag ttatctctgt ttaaacctgt caccccattg
      481 aaattacgta gtaaatcaag atctactata ttatttcctt gaaccacatc atgtttcatc
      541 aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct gtaatacaga
      601 aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa aggaaaagaa
      661 tctctgataa ggcattgaga taatggacat tcttagtgaa gaagaagtga agtactataa
      721 aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag acgcaccagt
      781 tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact gctgataaag
      841 gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt taacgcctta
      901 tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact ttccgtgtcg
      961 ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac agctggtgcc
     1021 gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagtgg tcctaccaac
     1081 acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct ggtatgacga
     1141 gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata ggctttagcc
     1201 gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac cagaacataa
     1261 agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg atctgcactc
     1321 atcacgcctg gttcctggta ggtgaaataa ctgctgggga agaaggctga tggggtcatg
     1381 ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt cacttcgctg
     1441 gtcagcgtca gggtcagttc gccaatgttt gccgggacgc tgtacgcagc aaccggacca
     1501 ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt ctgggtttta
     1561 gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat ggctggcgcg
     1621 tcgctggtgt tttgcggtac gtagctaaca tcggcaaggc taattccagg cgcgcttgcc
     1681 agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat tgttttcacc
     1741 tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat aatcagaggg
     1801 gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc gtaaacgcct
     1861 tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga aggtccactc
     1921 gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg ctttgccgta
     1981 gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat cccacttggc
     2041 gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag cctgccattg
     2101 ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg attcgacgtt
     2161 gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca tcgggcgaat
     2221 accgacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct ggtacatacc
     2281 cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag
     2341 catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg cgacgccaga
     2401 accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact gaacaacaaa
     2461 cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc agccgtcttt
     2521 cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt agtcgacacc
     2581 cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat cgaaaacgtc
     2641 gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg aagaaccacc
     2701 agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat cgatgttttc
     2761 cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat gaacgtcatg
     2821 acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt cgatcaggtt
     2881 tttaccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt cattctgttg
     2941 cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct ctttccacac
     3001 ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac ggtatttact
     3061 ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc agccaatacc
     3121 ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta cgcccgctgc
     3181 gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc ctgagtcatt
     3241 gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg
     3301 cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag atggcaacgc
     3361 tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac gtcgttctgg
     3421 cggtacacca ggttttgacg aatggaaggg atctggatat ggatttgggt ttcgttgccg
     3481 agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc actcggcagt
     3541 agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc acggctttca
     3601 actggcagcc agacttgctg acgatttggc atcggcagct ccacctgcac ttgatcgatt
     3661 gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat aaatccggcg
     3721 acaaaacggt ctgccggata gtggtacagc tccagcggtt tcccaacctg cgccacgcga
     3781 ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg atcgtgggtg
     3841 acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat acgcatttgc
     3901 acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac gcttggctcg
     3961 gccaccagcg tacggccaat cgccacacgc tgacgctgac caccggagag cgctttcggt
     4021 ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac gcgttggtta
     4081 atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc tgctactgac
     4141 aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc tgctggcgga
     4201 gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg
     4261 gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa caccacgaat
     4321 tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc ccaggctttc
     4381 gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa cctgtcatcg
     4441 acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa atcctccacc
     4501 ccctggcttt tttatggggg aggaggcggg aggatgagaa cacggcttct gtgaactaaa
     4561 ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga ttttatgtgc
     4621 gcatctccac attaccgcca attctgtaac agagatcaca caaagcaacg gtggggcgta
     4681 ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt ttaggtgttt
     4741 tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg tgcacgcatc
     4801 ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa
     4861 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     4921 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     4981 gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat cttctgggcg
     5041 cacgaccgtt ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     5101 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     5161 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     5221 aacccaccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     5281 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
     5341 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg atattaaaga cgtggccgtg
     5401 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg accttattaa aaacaaacac
     5461 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     5521 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     5581 gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg cgtgctgagc
     5641 gccggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct cgaaaactat
     5701 ctgctgactg acgaaggtct ggaagcggtc aataaagaca aaccgctggg tgcagtggcg
     5761 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     5821 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     5881 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     5941 gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg aacgccttgt
     6001 ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt cgcatcaggc
     6061 agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg
     6121 gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc agtgctaggt
     6181 ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg ggaatacctg
     6241 ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc caatcgtaaa
     6301 gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt cgtcctcttc
     6361 cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa ccagctgact
     6421 tttgaatgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa aacctataac
     6481 tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagtgacgg cgaaatcggc
     6541 aaaaattacc tctccgacgc gtttaaattt ggcggcgaac aaaaactgca actgaaagaa
     6601 acgaccgccc agcccgaagg cgaacgcgcg aatctacgcg tgattaccca gaatcgtcag
     6661 gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat gagctccctg
     6721 cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac gttgacgaat
     6781 aatcagagcg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca gtccattacc
     6841 gccgacggca actggggtga tgaaaagcta agccccggtt acaccgtgac tactggctgg
     6901 aaaaacttta cccgcgtctt caccgacgaa ggcattcaga aaccgttcct cgccattttc
     6961 gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt cggcatggtt
     7021 ctggcatgtc tggtgcagtg ggaagcattg cgcggcaaag cggtctatcg cgttctgctg
     7081 attctgccct acgcggtgcc atcattcatt tcaatcttga ttttcaaagg gttgtttaac
     7141 cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg
     7201 ttcagcgatc cgaccaccgc ccgcacgatg ctaattatcg tcaatacctg gctgggttat
     7261 ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga tttgtatgaa
     7321 gcctcagcaa tggatggcgc agatccgttc cagaacttct ttaagattac gctgccgctg
     7381 ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt taacaacttc
     7441 gtgctgattc aactgttaac caacggcggc ccggatcgtc ttggcacgac cacgccagcc
     7501 ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg cggcgggggt
     7561 caggacttcg gtctggctgc agcaattgcc acgctgctct tcctgctggt gggtgcgctg
     7621 gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata acaaaaatgg
     7681 caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg ctactgctac
     7741 tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctca ctgcgccagg
     7801 ggaactttgc gatcggcagc ctgatcccgg agcaaatctc ctgggatcac tggaaactgg
     7861 ctttaggttt tagcgtggaa caggctgatg gtcgcattac gccaccgcca ttcccggtac
     7921 tgctgtggct atggaactcg gtaaaggtcg ccgggatttc cgcgattggc attgtcgcgc
     7981 tctctaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc
     8041 tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc gcgttgtatg
     8101 cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact cacggcggcg
     8161 taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa ggctatttcg
     8221 aaaccatcga tagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca ccgtggcagg
     8281 ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta ttcatcctgt
     8341 cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt gacgtaaaca
     8401 gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac ctgtggggtg
     8461 actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc ttgctggctc
     8521 aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg
     8581 ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact gttattcggc
     8641 gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt
     8701 tccatcatag agcttattat ttttacgtta tttgttttcc cacttacgat aattctcttt
     8761 cgtgctctga gtcacggcaa tagtattgtt ttcatcaatt tttgataatt atcacaatta
     8821 agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc
     8881 tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc aggtctgaat
     8941 gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta cattaggtgg
     9001 tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac tcaataccgt
     9061 ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag gattttgcgt
     9121 agccagcgct ctgattggtt gcatcatcgg cggtgccctc ggtggttatt gcagtaaccg
     9181 cttcggccgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt ctggtgtagg
     9241 ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag tacctattta
     9301 tctggcaggt tatgtcccgg aatttgttat ttatcgtatt attggcggta ttggcgttgg
     9361 tttagcctca atgctttcac caatgtatat tgcggaactg gctccagctc atattcgcgg
     9421 aaaactggtt tcatttaacc agtttgcgat tattttcggg caacttttag tttactgcgt
     9481 aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg gctggcgtta
     9541 tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt ataccgtgcc
     9601 agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaaa gtatcctgcg
     9661 caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac actccctgga
     9721 tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga ttgtcatcgg
     9781 cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt actacgcgcc
     9841 ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga ccattattgt
     9901 cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata aatttggtcg
     9961 taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta gcctcggtac
    10021 cgcgttttac actcaggcac cgggtattgt ggcgctactg tcgatgctgt tctatgttgc
    10081 tgcctttgcc atgtcctggg gtccggtatg ctgggtaccg ctgtcggaaa tcttcccgaa
    10141 tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg cgaactactt
    10201 cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc atttccacaa
    10261 cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat ttatgtggaa
    10321 atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct gggaaccgga
    10381 aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac gccgcgccag
    10441 ttcggcgtgc tttcttttta ctcccgcttc agccgtttcg aattacacag ccacagagtg
    10501 atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga tttatgatcg
    10561 acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg tagcgggaag
    10621 tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata gagaaagtaa
    10681 accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa cagcacatct
    10741 gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag gcacagcagg
    10801 ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg acgagagagt
    10861 gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca aaaatgtgac
    10921 ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc gcctccacca
    10981 aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca tccggcaaga
    11041 caaaccgcac ccaagccggt aattcaaaaa tcacccattc aggtgattcc cagccaccag
    11101 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    11161 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    11221 tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagccc
    11281 aatgatggcc cagtcgctca ggcaatcaga cctgcaaccg atatcgacgg acaggaagac
    11341 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    11401 ctcaacaggc cattaagaat gagggtgcca gtgattgcga aaactggcac ctttcccatg
    11461 acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa cttacgccca
    11521 agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc
    11581 gggccagacg agaagagatc caacggtacc gagacataca cgcctttggt gaagtcccct
    11641 tcgccgtact cctcttttga aacattcgtg atcgtggcat agccacccac caccacgccg
    11701 ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag atactgcccg
    11761 acgctggctt tcactaacac gtcctgggcg aaagatggcg tccagtaggc ggtcagatgt
    11821 ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc
    11881 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg acgatacaac
    11941 acttcagccc ccgcaccgcc aaacatggtt tcgagataac ctccatagac ctgaccgtag
    12001 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata ggcatcgttc
    12061 tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg tggcggattg
    12121 gtgtagttaa acttgtcgta gttgttggca agatttgcaa acaggctgcc gatggtcagc
    12181 agatggtccg tcagccacaa atctgccgtt cccatcacgc ccagctgata catgtaaaag
    12241 ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc aaagcgtgat
    12301 ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac gcgtttttgc
    12361 gccagcgtcg tttcgtggcc caacggctct ccggcgagat gatttttcag gctggcgaca
    12421 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    12481 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcgcgcgaa
    12541 tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc ctggatctgc
    12601 ggatcggcaa gtccggcatt gtatttcagc aacgttaact gattcgccac caccgagtgc
    12661 tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc gatgtaggac
    12721 gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt gttgccacgt
    12781 tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac
    12841 ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattacc ttcatactcc
    12901 agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa cagtgaggcc
    12961 gggccgtgga acatctgact gccgtcgata gatcccgctt gtttgtagct gttatcgcga
    13021 tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct ggtgcccaga
    13081 tatccccagc ccaggccgag cgtaaaatcg aacggccccc aggctttgct ggcaacaaga
    13141 tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc cacttgcggc
    13201 agccagtaac tctcttccca caaacgcagt ttgagatcga aggctttatc tttatacgtt
    13261 tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc ggtgtaacgc
    13321 agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg gtactgatcg
    13381 ttatcgcgat agttcagact caactcccct tcccgtgcca tacgcgcggt gggagtttgt
    13441 aataatccta cgccaccgaa atccgactgc gacggaccaa tgggcgcagg atatgtttcg
    13501 ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct ttttttcatt
    13561 attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca tcaggcgtct
    13621 cactccagac ggagtctgcg aggccaacat aaataatgct gccgggcatc ggctctacgt
    13681 gacgcttgtt ccagtaagcc accggcgctt tttgcgtgcg tccgtccggg taaacgaccc
    13741 acgcgtagct gcgatccgca ccgctgagca ggctctggtc agagagatag ctcgccacgt
    13801 cgcgacctgg agtgaatggc tgattgccag gatggctgat aagcccgaac aacgtgaccg
    13861 tggacggcgg tggtccgacc cacagcgtgt agttgccctg caacggcggg ttaccgcgtt
    13921 cggcaacgcg gacgatatcg ggatcaagat tgattttttg cctgcctgtc acttccaatg
    13981 cctggatttg ctggcgtaag gcgttaatgg cggcggcatc gtcggtgctg gaatctgcgc
    14041 cctgttctgc cagccgtgtc agcaacgcct gctgctgacg caatgctgcc gccgttgcca
    14101 gctcttcgct aatcaccgca ccaggccacc agctgtttgc cagtcgaggt tgccctacca
    14161 gatcgattaa atgctctgcg cccgttagcg ttttggcctc actgctgcca ttgctgaaca
    14221 ccttgacggt tcctgccgcg aagaccgatg acgctcccac actcaaaagc aacgcgacaa
    14281 tagtttgttt aatcattgtt ttgccgcctt gatgagagtg gttttcaccg ggaaataatc
    14341 cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc agtagcggtt
    14401 atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaacca gggtttcatc
    14461 gctgccgacc ttgacggtat cggtgccatc ccatttgaag actgagcgtg cggtggcgta
    14521 gcgtacctgc tggtactcgg tccagcccat cgtgcgcgtc catgttgcac catcaacaat
    14581 ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca ggttgtcgcc
    14641 gccaagcaaa gtcttcacca gacgaccatg ttgtgtgaca agagtggctt gatcctgagt
    14701 gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaacaatt gcggcccgcc
    14761 attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt ccgtcagctg
    14821 tacgcctggc gtgccgaaca gactgtccca cagtgaattg cccagctctt tagtcgtggc
    14881 tgaacaggcc tgtaataaca ggcagataag aatgagtgca ggtcgcttca cgactcttct
    14941 ccagaggggc agaataacca caccggagtg tggttatggt taatcccttc atatttcaag
    15001 ccacagatgc aatgactcga attattttgg ggatacatac tttattactg ggtactggtg
    15061 gttgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccggttgc cgcgagcgcg
    15121 acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactccc cgccgaaacc
    15181 gacgttgctg ccgcccctgc cgcttcgccc acctgtacag gtgcagccca cacagaagtc
    15241 gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac aatttccctt
    15301 cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta tacacaacta
    15361 aagcatgcga attacggcgc ggggaattag cgatggtgtt ttaggataat tggaatgaat
    15421 atcaaatata agtttcaact tatatttaaa taacaataaa aatcataaac ttaaaataaa
    15481 aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa tctattttag
    15541 gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat ctgataaaac
    15601 aatgccgact tattgtcggc attgttttta aggttattta actcctcata ctatgcatta
    15661 atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct tatccggcct
    15721 acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag cggttaatca
    15781 gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc tctggcagaa
    15841 tacggttcgc cagttgttta cccaactcca cgccccactg gtcgaaggta aagatgttca
    15901 ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca cccaggctga
    15961 acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
    16021 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat tcctgctcaa
    16081 ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag ttagacagca
    16141 gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg aaatcgcacg
    16201 gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg ttagtgcctg
    16261 gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta cggtcaacat
    16321 acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact
    16381 ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attgttgtac cagatgccaa
    16441 tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
    16501 tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag agaacaatcg
    16561 acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag aactcgaaca
    16621 tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa agcgccgcaa
    16681 agtgttttgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca cgcgcgctat
    16741 gggcgttggt catagtttcc tgagtggtga aggttttaga tgctaccagg aacagcgtgg
    16801 tttccgggtt tacttttttc agcacttcag cgatgtgagt cccatcgacg ttagaaacaa
    16861 agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg tacgggccga
    16921 ggtcagaacc accgatcccg atgttcacta cgtcagtgat tgctttgccg gtataaccct
    16981 tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc accgcgttga
    17041 cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
    17101 ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
    17161 taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc agcgtctctt
    17221 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt gcggagaact
    17281 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
    17341 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
    17401 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
    17461 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagtgaa
    17521 gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca tgaccccgtc
    17581 gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
    17641 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
    17701 gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct
    17761 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
    17821 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
    17881 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
    17941 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
    18001 gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg tatcactaat
    18061 ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa actcgacgct
    18121 attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
    18181 gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc gctggcaacg
    18241 tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac cctgctgttt
    18301 gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
    18361 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
    18421 gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggt
    18481 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
    18541 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
    18601 tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc
    18661 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
    18721 gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt
    18781 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
    18841 aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg
    18901 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa
    18961 gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
    19021 acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga agaaggtctg
    19081 gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
    19141 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac
    19201 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact gcatagtaat
    19261 ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt gattttcttg
    19321 tcactatgct tatcaataaa cgagcctgta ctctgttaac caacgtcttt atcgagaata
    19381 attgccttta atttttttat ctgcatctct aattaattat cgaaagagat aaatagttaa
    19441 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
    19501 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
    19561 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
    19621 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
    19681 agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg
    19741 atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctaatgaa
    19801 caatcaacgc ctgagatttt cactcgtcag tgtgaacaca attttcccct caccctaacc
    19861 cttttcccga aggggcgagg ggactgtccg agcacttttg ggccgttttc atcagtctga
    19921 ggcgttaatc agcgcccctt ctttttacgc cccggcgagg taaaacgctt accgttggac
    19981 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgccc
    20041 gctgttttcg gtttggcctt cgctttgggc ttcgcctcgg aagaggaatt ttcaatgagc
    20101 ttaaagagat cgataagctc gtcgtcggtt aaatcgcgcc attctcccag cggaatgccg
    20161 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata gccgaaatgc
    20221 tcgcacatgc gccggatctg acggttcaac ccctgcacca gggtaatgcg gaagacaaac
    20281 ggtgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg cactcccgcc
    20341 cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc
    20401 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
    20461 atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
    20521 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc
    20581 ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt
    20641 actttcacaa tgtcgccggg tttcacctga tcaccaatgg tggctcgctt gccattaagg
    20701 aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
    20761 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc
    20821 ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa
    20881 tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct
    20941 ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg atgactacta
    21001 cgcagagaaa caaatgtgca agagaaagcg ctatttctcc ccatgcaaag agtgaaaaat
    21061 cgctttgaaa ggccctcata atggggttat atcagatcaa gcggtgagaa agcgtatttg
    21121 cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt
    21181 gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg cgcgcgaact
    21241 gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt
    21301 caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc aactgaagca
    21361 aaagtttggc ttgaaagacg ttgtggtggt gtcgggaaat gatgaggatg aagagactca
    21421 actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg agcctggcga
    21481 tattgtcggt ttttcctagg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaagc
    21541 ggggcaatcg cggcagttaa tctgcgtgcc aattattggc ggaccgtccg gtaaactcga
    21601 aagccgctat cacgtaaaca cattaaccta cagcgtggca gcgaagctga aaggggaatc
    21661 gcatctcgcg gattttccgg ctctgctgga taacccctta attcgtaatg ggatcatgca
    21721 gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg
    21781 aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga
    21841 gagtgacgac ctgaaagcag gcgcggtatt ccattggcat tgccatgagt gaagaaaaat
    21901 acagcggaat tgttggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca
    21961 gcacagctga actattactg aaataaaaat cttcccgatg tttaccgtaa taaaaattac
    22021 ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg
    22081 ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt gtagaggaat
    22141 tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat ggtcaatatg
    22201 aagttcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa gaggtaaatc
    22261 atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc aataacgccg
    22321 gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca
    22381 acgaagctgc attcgaaaaa atggtcaata tcaaccaaaa aggcgttttt ctgatgtcgc
    22441 aggcggtggc gcgccagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag
    22501 aaagtggact ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa gccgcgctca
    22561 atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt gtggtcggta
    22621 tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa gaggcgctgg
    22681 cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc
    22741 ctattgggcg cgccggaaga ttagcagaag tcgctgattt tgtttgttat ctgctgtctg
    22801 aacgcgccag ctacatcacc ggagtaacca ctaacattgc gggcggcaaa acgcgcggat
    22861 aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct tgcgccatgc
    22921 tggaatcggt tcagatggtg tacggcgacg ccaatgtcga ggcggttgaa tttgtaccag
    22981 gcgagaacgc cggagacatc gtagcaaagc tggaaaagtt agtaagcatt cacaatcaag
    23041 atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc
    23101 ttgcgatgcg caatcctcgt ctacgggtaa tcagcggcct ttccctgccg ctggcgctcg
    23161 aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa
    23221 tcgcaaagca aacctgcgtt gtctggaaac aactggcaac ggcagaggaa gatttctgat
    23281 gaacattacg ctcgcccgca ttgatgaccg tctgatccac ggccaggtca ctaccgtctg
    23341 gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt ataacgatga
    23401 agtgcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa
    23461 tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat taggacgaaa ccgtttttta
    23521 tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa aaataggcac
    23581 cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca aagcggtttc
    23641 attagatgat gatgatatta acgcatttca tgagttaaat aatcttggcg tcataatgga
    23701 tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa acgaacagtt
    23761 aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg gatgacgaca
    23821 cttaaaaggt gacaaatcat ggaaataagt accctacaaa ttattgctat atttcttttt
    23881 tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg cccgttaatt
    23941 gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat actcggtggt
    24001 acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct
    24061 gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc
    24121 ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccaag tgctgacggt gtttgcccgt
    24181 accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaggc gcggtttcgc
    24241 actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt tgctattccg
    24301 gcactgattg tctcactgtt cgtcagcgcc gatatggtca gcaatatgtt aaacgccatt
    24361 cccgaatttg tgacccgtgg actgcaaatt gctggcggtt ttatcgtggt ggtcggttac
    24421 gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc
    24481 ctcgcaggtg gctacctcga tctcagcctg ctggcgttcg gtggcgtcgg cgtgatcatg
    24541 gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca actccaggcc
    24601 accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg aacagagaaa
    24661 aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcgtcctt
    24721 taactttgaa cgtattcacg gactgggctt ttgctacgac atgatccccg ccatcaagcg
    24781 actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa
    24841 taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg aagaggcgcg
    24901 ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg gtctgatggg
    24961 accattggca ggagttggcg atccactggt ctggggaacg ctgcgcccga ttaccgctgc
    25021 gctcggcgca tctctggcac tttctggcaa catcctcggc ccgctgctgt ttttctttat
    25081 tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg
    25141 ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc
    25201 gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc
    25261 gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcatcatga ccgtgcagaa
    25321 cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctac tgatggttcg
    25381 tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg gcttagggat
    25441 tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac tgccggggcc
    25501 atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg gtaaacgtga
    25561 tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt
    25621 ggtcactgac agcctgtgcc tctcttcctg gaaagaggca acctgggtga aaaccataaa
    25681 aaagtacccg acgatgtggc gaccaaccca atcatcatcg gccacgagtt ttgcggcgat
    25741 attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg ttatgtgatt
    25801 caggccaacc tgaaactccc tgaccgcccg gactgccccg gctactcctt cccgtgggtg
    25861 ggcggcgaag ccacgcatgt ggttattccc aacgaggtca tggaacaaga ttgcctgctg
    25921 gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt
    25981 ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac gatggggatt
    26041 cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac caatgggatt gttggcgatt
    26101 gattatgcgc tgcatggacc agttaacccg tcgctgctcg tcattaccga taccgacaac
    26161 gacaaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact gattcattat
    26221 ctcaatgcca ccgatgcagc atttgatacg ttaatggcgc tgagtggcgg tcacggcttc
    26281 gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc ttccttgctg
    26341 gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca
    26401 attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc aggcggcaat
    26461 accgacgaca tgcgcgcggc ggtcaaactg attgaagaga aaaaagtgca ggccgcaaaa
    26521 gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc
    26581 gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgctgac gtcactcacg
    26641 cagattcagg atcaagcact ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt
    26701 gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta atcgcgctac
    26761 ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgacctgat gtgcggctga
    26821 ttgctgaagc ggcccaatgt gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg
    26881 ctcaaattgg tggcaaagta cacattgttt atgccggatg cggaataaag ccttattcgg
    26941 cctacataat ctcgaaaatt caatatattg cagttatctg gtaggcctgg taagcgtagc
    27001 gcatcaggca atgctgtgtt tgtcatcagt ctcagcgccc ggtgcaatac gtgacatcgt
    27061 gagccatgtc agtaagtggt taaattcgca gtggtagcat gccttccgtg atttcttact
    27121 aaccggagaa gtaatggaac tgcttttatt gagtaactcg acgctgccgg gtaaagcctg
    27181 gctggaacat gcactgccgc taattgctga acagttgcag ggtcgccgct cagcggtgtt
    27241 tatccctttc gctggcgtaa cgcagacctg tgatgattac acagcgaaaa cggctgcggt
    27301 tctcgctccg ctgggtgttt ctgtcaccgg tattcatagc gttgtcgatc ccgttgccgc
    27361 gattgaaaat gctgagatcg tgattgtcgg ggggcgggaa tactttccag ttgctgaaac
    27421 agtgccgcga gcgcgggctg ctggcaccaa ttactgacgt ggttaaacgt ggtgcgctgt
    27481 atattggctg gagcgcaggc gctaaccttg cttgcccaac cattcgtacc accaacgata
    27541 tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct gttcccgctg caaatcaacc
    27601 cgcacttcac caacgcgctg ccggaaggcc ataaaggtga aacccgtgag cagcgtattc
    27661 gcgaactgct ggtcgtcgcg ccagaactga cgattattgg tctaccggaa ggtaactgga
    27721 tcacggtgag taaaggtcac gctacgctgg gtggcccgaa caccacttat gtgtttaagg
    27781 ccggtgaaga agcggtgccg ctggaagcgg gtcaccgttt ttaagtgaat tgccggatgc
    27841 atgatccggc atcttgttca tagtgcctga tgcgacgctt gacgcgtctt atcaggccta
    27901 caggattcac aatattaata ctcgtcccgt ccttcatctt catccggctg ttccagcaca
    27961 ctgtaagcca ccgaacaaaa cagcgagttc agacgctgca tatctcccag taagcctaaa
    28021 tgtagcgaac tggtttcaat gctctgcacg ttttgctgat gcaggcgatc gacgtgggcg
    28081 tgggaatagc ggcgattaag aatgcgaaag cgatgcttgc tgcggcgcaa acgacgagcg
    28141 ctggtgacat cgccagagaa gaacaccgac attgccagct ttaaattact gagcaattgc
    28201 tcatagagcg catccagttc cttcaaccca tcaagcgaaa atgcccgccg tgctgccagc
    28261 gatttatcag caatttcgct gcccatgcgc tcgacgatat cggaagcctg ttcaaggttg
    28321 agcgacattt caataatctc cgcccagcga cgcgactctt cttctgccag ttcctctttt
    28381 ggcattcgcg ccagatacag cttaatggcg gtgtagagaa cgttgatatc atctgccagc
    28441 ttacgcagct ctttctcctg ccgtggctcg ccgtgcatca ctttattcag cccttccatc
    28501 atctgttcca tggcgtcgcc aatgcgcagg gtttcgcgcg cggcgttcgc cagagcaagc
    28561 gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc gtagctgggt atccagttcc
    28621 ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg ggtcaacaaa tggcagcatg
    28681 accaggcaac gtacaaggtt gtagaagacg tggaaataga tcaccagttc cgcttttggc
    28741 aatgacaact tccccattgt ctctgccagc aaatggacaa acggcaggat aatcaggcta
    28801 cccaccagct taaacagcaa actacccagc gcgacacggc gggctgcggc attggcggca
    28861 ctgttgttga gcatcgccag caggccggaa ccgaggttag caccaatcac cagacagagc
    28921 gccacgggga aggagataat gcctgcggcg gtcagagtcg cggtcagcag tacagcagca
    28981 aggctggagt agctgataat ggcgaacatc gcgccaatca gcgcatccag cagaatatcg
    29041 ccggtcagcg aggcaaagat cacctgaacg ccgtttgcct gggtgatcgg cgttacggcc
    29101 tgcacaatca actccagcgc cagcaaaatc agcccaagac caataccgac gcggcccagt
    29161 tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa aataagtaac
    29221 ggtgacagcc aggataagtc gaaggtgaga atacgcgcca ttagcgccgt cccgacatct
    29281 gcacccagca caatcaccag agccggtgcg agggctacca gatcctgagc gacgaacgag
    29341 gtcaccagca tggtggtggc attactgctc tgtaccagtg cggtaacgcc gatccccgcg
    29401 caaaaggcga gcggcttttt ttcgacgctc cggctaagga cagtacgcaa acgcgcgccg
    29461 aagacgcgca ttacgccggt tcgaacaata tgagtccccc agaccagcag ggcgacggca
    29521 gaaagcaggt gaagcagcgt taacacgagt tcaggttctc cttatcgtta tatgttcctg
    29581 aagcaattga atgaccaaag gaccggaaac acgctgactg gcatctttac tgtgcctttt
    29641 gacattcgtt tttccggcta agattattcc agcctgtttc cagtataagt gtttcatatt
    29701 aataaagaga cagggcactc attgagcgcc ctgtttgttg taaggaaaag tgacagattt
    29761 gtgaatcagt ccgcgtcata ccccagattc ggtgccagcc agcgctcaac atcggagacg
    29821 ctcatacctt tacggcgggc atagtcttca acctgatcgc gctgaatttg cgccaccgcg
    29881 tagtacttgc tgtccgggtg gctgaaatac caacccgaaa ccgatgcacc aggccacatg
    29941 gcgaaagatt ctgtgagttt catgccagta tgtttttcca cttccagcag ctcccagatg
    30001 gtggcttttt ccgtatgttc cgggcaggcc gggtagcccg gtgccggacg gatgccctgg
    30061 tagttttcgc ggatcagttc ttcgttgctg aggttctcgt tcggcgcata gccccagtag
    30121 actttacgca cacgctcatg gagatactcc gcaaaggctt cggctaaacg gtcggcaagc
    30181 gctttcacca tgattttgtt gtaatcatcg tgctgcgctt caaaggcatc agccagtgcg
    30241 tcctcttcca gcccgccagt cacggcaaat gcgccgatgt aatctgcttt accagaaagc
    30301 ttcggcgcaa cgaagtcagc gagacagtag ttagcgaatc ccgttttttc ggtctgttga
    30361 cgcagatggt ggctgacgtt gatcacatgg gtgcgcgttt cgtcacggta gatttcaatg
    30421 tcatcgccca cacggtttgc cgggaacaag cccaccacgc cacgcggatt cagcgttttc
    30481 tcggcgctta atttatccag catgtcgttg gcgtctttaa acagccgctg cgcctcaacg
    30541 cccaccactt catcttccag aatgcgcgga tacttcccgg ccagcgacca ggtcataaag
    30601 aacggtgtcc agtcgatgta attacgcaga gtttcgatgc tggcttcaac ttcctgcacg
    30661 ccgagacggt gcgccaccgg cggcgtgtaa gcctgccagt caaaagcgaa gtcgttatcg
    30721 cgcgccgctt ccagcgtgac cggtggtgtg cgcggtttct tgcgcccgtg ctgaatacgt
    30781 acggtttcat actccttgcg ggtacgagcg acaaaatcat cgcgctgagt atcggaaagc
    30841 agcgccgcca ccacaccaac ggtgcgcgag gcattctgca catacaccgt cgggccgctg
    30901 tagttctgct caattttcac cgccgtgtgc gcttttgagg tcgtcgcgcc accgatcagt
    30961 aacggaatgg tgaagccctg acgctccatc tctttcgcca cgttaaccat ctcgtccagc
    31021 gacggcgtga taagccccga aagtccaatc agatcagcat tcacttcttt agcggtacgg
    31081 agaatttttt ccgcaggcac cataacgccg agatcgacaa tttcgtagtt gttacattgc
    31141 agcaccacac caacgatatt tttaccgatg tcgtggacgt cgcccttcac ggtggcgatc
    31201 accatcttgc cgttggtttt gccctgctct ttgctggctt caataaacgg ttcgaggtag
    31261 gccactgcct gtttcatgac gcgcgccgat ttgaccacct gtggcaggaa cattttcccc
    31321 tcgccaaaca gatcgccgac cacgttcatg ccgtccatca acgggccttc aatcacttca
    31381 atcgggcgcg tagcctgctg gcgggcttct tcggtatcct gctcgataaa ctcagtaatg
    31441 cctttgacca gcgagtattc cagacgttta ttcacttccc acgagcgcca ctccgcctgc
    31501 tgggcgttag cggtgtcgtc ggttttgctg ccgcgatatt tctcggcaag ctccagtaaa
    31561 cgttcagtgc catcatcgcg acgattaaga atcacatctt ccaccgcgtc gcgcagttca
    31621 gcgggcaggt cgtcgtaaat cgccagttgc ccggcgttga cgatccccat atccatgcca
    31681 ttgcgaatag cgtagtagag gaacaccgcg tgaatggctt cgcgcaccgg ttcgttgcca
    31741 cggaatgaga aagaaacgtt agatacgccg ccggaaatca gcgcgtgcgg cagttcgcgt
    31801 ttgatgtctt cgcaggcgcc gataaagtcc tgcgcatagt tgttgtgctc ttcaatgccg
    31861 gtggcgaccg cgaagatgtt cgggtcgaag atgatatctt ctggcgggaa gccaacctct
    31921 tcggtgagga ttttgtacgc ccgacggcaa atctcgattt tccgtgcgcg ggtatcggcc
    31981 tgtccctgtt cgtcaaaggc cattaccacc accgctgcac cgtaacgacg caacagtttc
    32041 gcatgatgga taaaggcgtc gacgccctct ttcattgaga tagagttaac aatgcctttg
    32101 ccctgaatac acttcagccc cttttcgatg acctcccatt ttgaggagtc gatcataatc
    32161 ggcacgcgag cgatatccgg ttcaccggca atcagattga gaaaacgcac catcgccgct
    32221 tcggcgtcga gcatcccttc atccatattg atatcgataa tctgcgcgcc gttttccacc
    32281 tgctggcggg ccacatccag tgcctcgctg tatttctctt ctttgatcag gcgcttgaac
    32341 ttagcggaac cggtgacgtt ggtgcgttca cccacgttca caaacaggct atcttcgcca
    32401 atgttcagcg gctccaggcc ggacaaacgg caggctacgg ggatttccgg cagtttgcgc
    32461 ggcgctaatc cttctactgc acgactcatc gctgcaatat gttgtggcgt ggtgccacag
    32521 cagccgccga cgatattgag aaagcccgct tccgcccatt cacgtatttg ttttgccatc
    32581 gtgtcggcgt cgagatcgta ctcaccaaag gcgttgggta gcccggcgtt cgggtgcgcg
    32641 gtgacgtagc attccgcaat ccgtgacagc tcctgcacgt actggcgcag ttcatcgggc
    32701 cccagcgcac agttcaggcc aaaggtcagc gcttcggcgt ggcgcaatga gttgtaaaat
    32761 gcttcggtgg tctgcccgga gagcgtgcgc ccggaggcgt cggtgatggt gccagagatc
    32821 ataatcggca gctcaacgcc cagcgtttca aactccgttt ttaccgcaaa taccgccgct
    32881 ttggcgttaa gggtgtcgaa aacggtttca atcaggatca gatccgcacc accttccacc
    32941 agcgctttgg tggactctcg ataagccacc accagcccgt caaaagtgat attacgaaat
    33001 gccggatcgt tgacgtccgg agaaatagac gccgtgcggt tggttgggcc gagaacaccg
    33061 gcaacgtagc gcggtttctc cggcgtgcgc gcggtccact cgtcagcaca ggcgcgcgcc
    33121 agtttcgccg ccgcaaagtt gatttccgcc gacagggatt ccatctggta atccgccatc
    33181 gcaatggtcg tggagttgaa ggtgttggtt tcgatgatat ccgcgcccgc ttcaaagtag
    33241 gcgttgtgga tagcggcgat cacttccggt ttactgagta ccagcaggtc gttgttgcct
    33301 ttgaggtcgc atggccagtc ggcaaagcgt tcaccacgaa aatcggcttc gttcagtcga
    33361 tagctctgga tcatggtgcc cataccgccg tccagcacca gaatacgttc atttaactgc
    33421 gcacgcagtt gttccacttt gctgctcaca cttgctcccg acacgctcaa cccagattta
    33481 ataaaaattc aacaaaccat actggcataa acgcatctgt ggtaaaagcg accaccacgc
    33541 aacatgagat ttgttcaaca ttaactcatc ggatcagttc agcaactatt gcattaacta
    33601 acaataaaaa tgaaaatgat ttccacgata cagaaaaagg agactgtcat ggtcgcaccc
    33661 attcccgcga aacgcggcag aaaacccgcc gttgccaccg caccagcgac tggacaggtt
    33721 cagtctttaa cgcgtggcct gaaattactg gagtggattg ccgaatccaa tggcagtgtg
    33781 gcactcacgg aactggcgca acaagccggg ttacccaatt ccacgaccca ccgcctgcta
    33841 accacgatgc aacagcaggg tttcgtgcgt caggttggcg aactgggaca ctgggcaatc
    33901 ggcgcacatg cctttatggt cggcagcagc tttctccaga gccgtaattt gttagcgatt
    33961 gttcacccta tcctgcgcaa tctaatggaa gagtctggcg aaacggtcaa tatggcggtg
    34021 cttgatcaaa gcgatcacga agcgattatt atcgatcagg tacagtgtac tcacctgatg
    34081 cgtatgtccg cacctatcgg cggtaaattg ccgatgcacg cttccggtgc aggtaaagcc
    34141 tttttagccc aactgagcga agaacaggtg acgaagctgc tgcaccgcaa agggttacat
    34201 gcctataccc acgcaacgct ggtgtctcct gtgcatttaa aagaagatct cgcccaaacg
    34261 cgcaaacggg gctattcatt tgacgatgag gaacatgcac tggggctacg ttgccttgca
    34321 gcgtgtattt tcgatgagca ccgtgaaccg tttgccgcaa tttctatttc cggaccgatt
    34381 tcacgtatta ccgatgaccg cgtgaccgag tttggcgcga tggtgattaa agcggcgaag
    34441 gaagtgacgc tggcgtacgg tggaatgcgc tgactttttc tggcgggcag aggcaatatt
    34501 ctgcccatca tacctgagtg gcaatagaat aagggtgtct gttaatcgca ttgacgccaa
    34561 aataacttaa tgtcatacac ttcacacact tttattaact cagcattatt tttaaacatc
    34621 aaaccactta attattaaca aaatcgcaag atctcattcc accgaaactt ttacggctgc
    34681 attatgagat tgagcgattt atgatatttc tttagttgta atcatattaa attttttcac
    34741 tgttctgata gttaaaattt aagacatcaa taaacaatga gatatttaaa tgattactcg
    34801 tattcctcgt agttctttct ctgcaaatat taataataca gcccagacaa atgaacacca
    34861 aaccctgagt gaattgtttt ataaagaact cgaggataaa ttttctggca aggagctggc
    34921 gacgcctcta ttaaaaagct tctcagagaa ctgtagacaa aatggtcgtc atatctttag
    34981 caacaaggat tttgtcatta aattttccac gtccgtctta caagctgata agaaagaaat
    35041 tacgataatt aataaaacga aaacacgaca ctcactcaaa ccattgctcc aatatttgaa
    35101 gaatacctaa tggaaatttt acctcaacgc tcagacactc ttgataaaca agaattaaac
    35161 ctaaaatcag atagaaaaga aaaagaattc ccaagaatta aacttaatgg tcaatgctat
    35221 tttccggggc gaccccaaaa ccgtatagta tgccgacaca ttgctgcaca atatattaat
    35281 gatatttatc agaatgttga ttacaaaccc catcaagatg attattcttc agctgaaaaa
    35341 tttctcactc acttcaacaa aaaatgcaaa aaccagactt tggcgttgat ttccagccgt
    35401 cctgaggggc gttgcgttgc tgcctgcggt gatttcgggc tagttatgaa agcatatttt
    35461 gacaagatgg aatcaaatgg catcagtgtt atggcagcca tattactggt ggataaccat
    35521 gctttgacgg tccggctaag aataaagaac acaactgaag gatgtaccca ttacgtggtt
    35581 tcggtttatg atcctaatgt aactaacgac aaaataagaa tcatgagcga aagcaaagag
    35641 gatattaaac actattctct gatggatttt atgaatgtag attatagcct cctgaaatgg
    35701 tcaaatgatc atgttattaa ccaatctgtt gcaataattc cagcacttcc gaaagaacag
    35761 ctattgatat taaaagga
//
LOCUS       DAETQN010000032        34981 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.032, whole genome shotgun sequence.
ACCESSION   DAETQN010000032 DAETQN010000000
VERSION     DAETQN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.032"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..815)
                     /locus_tag="K0W68_001578"
     CDS             complement(<1..815)
                     /locus_tag="K0W68_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBI3073170.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="K0W68_001579"
                     /pseudo
     CDS             907..993
                     /locus_tag="K0W68_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1397..1873)
                     /locus_tag="K0W68_001580"
     CDS             complement(1397..1873)
                     /locus_tag="K0W68_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBI3073171.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="K0W68_001581"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="K0W68_001581"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBI3073172.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="K0W68_001582"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="K0W68_001582"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBI3073173.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="K0W68_001583"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="K0W68_001583"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBI3073174.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="K0W68_001584"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="K0W68_001584"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBI3073175.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6911
                     /gene="bioD"
                     /locus_tag="K0W68_001585"
     CDS             6234..6911
                     /gene="bioD"
                     /locus_tag="K0W68_001585"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBI3073176.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7490..9511
                     /gene="uvrB"
                     /locus_tag="K0W68_001586"
     CDS             7490..9511
                     /gene="uvrB"
                     /locus_tag="K0W68_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBI3073177.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="K0W68_001587"
     CDS             complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="K0W68_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBI3073178.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10876..11020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11008..11997
                     /gene="moaA"
                     /locus_tag="K0W68_001588"
     CDS             11008..11997
                     /gene="moaA"
                     /locus_tag="K0W68_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBI3073179.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12019..12531
                     /gene="moaB"
                     /locus_tag="K0W68_001589"
     CDS             12019..12531
                     /gene="moaB"
                     /locus_tag="K0W68_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBI3073180.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12534..13019
                     /gene="moaC"
                     /locus_tag="K0W68_001590"
     CDS             12534..13019
                     /gene="moaC"
                     /locus_tag="K0W68_001590"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBI3073181.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13012..13257
                     /gene="moaD"
                     /locus_tag="K0W68_001591"
     CDS             13012..13257
                     /gene="moaD"
                     /locus_tag="K0W68_001591"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBI3073182.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13259..13711
                     /gene="moaE"
                     /locus_tag="K0W68_001592"
     CDS             13259..13711
                     /gene="moaE"
                     /locus_tag="K0W68_001592"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBI3073183.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13848..14552
                     /locus_tag="K0W68_001593"
     CDS             13848..14552
                     /locus_tag="K0W68_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBI3073184.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14757..15470
                     /locus_tag="K0W68_001594"
                     /pseudo
     CDS             14757..15470
                     /locus_tag="K0W68_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(15535..16246)
                     /locus_tag="K0W68_001595"
                     /pseudo
     CDS             complement(15535..16246)
                     /locus_tag="K0W68_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="K0W68_001596"
     CDS             complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="K0W68_001596"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBI3073185.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17484..18245)
                     /locus_tag="K0W68_001597"
     CDS             complement(17484..18245)
                     /locus_tag="K0W68_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBI3073186.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18378..18788
                     /locus_tag="K0W68_001598"
     CDS             18378..18788
                     /locus_tag="K0W68_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBI3073187.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18750..19856)
                     /locus_tag="K0W68_001599"
     CDS             complement(18750..19856)
                     /locus_tag="K0W68_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3073188.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19867..21000)
                     /locus_tag="K0W68_001600"
     CDS             complement(19867..21000)
                     /locus_tag="K0W68_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3073189.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="K0W68_001601"
     CDS             complement(20993..22729)
                     /locus_tag="K0W68_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3073190.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="K0W68_001602"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="K0W68_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBI3073191.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="K0W68_001603"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="K0W68_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBI3073192.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="K0W68_001604"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="K0W68_001604"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HBI3073193.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="K0W68_001605"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="K0W68_001605"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HBI3073194.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="K0W68_001606"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="K0W68_001606"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBI3073195.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="K0W68_001607"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="K0W68_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBI3073196.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="K0W68_001608"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="K0W68_001608"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBI3073197.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(31413..31673)
                     /locus_tag="K0W68_001609"
     CDS             complement(31413..31673)
                     /locus_tag="K0W68_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI3073198.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="K0W68_001610"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="K0W68_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBI3073199.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="K0W68_001611"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="K0W68_001611"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBI3073200.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34981)
                     /locus_tag="K0W68_001612"
                     /pseudo
     CDS             complement(32997..34981)
                     /locus_tag="K0W68_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8273 a         8976 c         9488 g         8244 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggatct gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatccagaaa atgcggcaac cggaaagtac ataaaccttg
     6901 ccttgttgta gccattctgt atttggttaa attgcgagcg agatcgcgtc ttcgattgac
     6961 tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa aattttactt ctgcctcatg
     7021 cggcgaatgt gggaattgcc caggcggcgg gggatagggg ctggagacag ttatccacta
     7081 ttcctgtgga taaccatgtg tattagagtt agaaaacacg aggcaagcga gagaatacgc
     7141 ggcttgcacg cgaattggcg ttaaagacgg ctcaaagaaa tatcttttat tttttaactg
     7201 gttagataaa tgcaatggca gtcactgaac aggcatctct tgccataaaa ctgtcatcac
     7261 tcatcttgac aaatgttaaa aaagccgttg ctttggggat aacccggtaa ggccggagtt
     7321 ttatctcgcc acagagtaaa ttttgctcat gattgacagc ggagtttacg ctgtatcaga
     7381 aatattatgg tgatgaactg tttttttatc cagtataatt tgttggcata attaagtacg
     7441 acgagtaaaa ttacatacct gcccgcccaa ctccttcagg tagcgactca tgagtaaacc
     7501 gttcaaactg aattccgctt ttaaaccttc tggcgatcag ccagaggcga ttcgacgtct
     7561 cgaagagggg ctggaagatg gcctggcgca ccagacgtta cttggcgtga ctggctcagg
     7621 gaaaaccttc accattgcca atgtcattgc tgaccttcag cgcccaacca tggtacttgc
     7681 gcccaacaaa acgctggcgg cccagctgta tggcgaaatg aaagagttct tcccggaaaa
     7741 cgcggtggag tatttcgtct cctactacga ctactatcag ccggaagcct atgtaccgag
     7801 ttccgacact ttcattgaga aagatgcctc ggttaacgaa catattgagc agatgcgttt
     7861 atccgccacc aaagcgatgc tggagcggcg tgatgtggtt gtagtggcct ctgtttccgc
     7921 gatttatggt ctgggcgatc ctgatttata tctcaagatg atgctccatc tcacggtcgg
     7981 tatgattatc gatcagcgcg cgattctgcg ccgactggcg gagctgcaat acgctcgtaa
     8041 tgatcaagca ttccagcgtg gtactttccg cgttcgtggc gaggtgatag atatcttccc
     8101 ggcagaatcg gatgacattg cacttcgcgt ggaactgttt gacgaggaag tggaacgatt
     8161 gtcgttattt gacccgctga ccgggcagat tgtttccact attccacgtt ttaccatcta
     8221 cccgaaaacg cactacgtca caccgcgcga gcgcatcgta caggcgatgg aggagatcaa
     8281 agaagaactg gccgccagac gcaaagtgct gttggaaaac aacaaactgc tggaagagca
     8341 gcggctgacc cagcgtaccc agtttgatct ggagatgatg aacgagctgg gctactgttc
     8401 ggggattgaa aactactcgc gcttcctctc cggtcgtgga ccgggtgagc caccgccgac
     8461 gctgtttgat tacctgcctg ccgatgggct gctggtggtc gatgaatctc acgtcaccat
     8521 tccacaaatt ggcggcatgt atcgcggtga ccgggcgcgt aaagagacac tggtggagta
     8581 cggcttccgc ctgccatcag cgctggataa ccgtccgctt aagtttgaag agttcgaagc
     8641 attagcgccg caaaccatct atgtttcggc gacgccgggt aattacgagc tggaaaaatc
     8701 cggcggcgat gtggtggatc aggtggtgcg tccaaccgga ttgcttgacc cgattatcga
     8761 agtgcggccg gtggcgacac aggttgatga tcttctttcg gagattcgtc agcgagcggc
     8821 aattaacgaa cgcgtactgg tcaccacact gaccaagcgg atggcggaag atcttaccga
     8881 atatctcgaa gaacatggcg agcgcgtgcg ttatcttcat tcagatatcg acaccgtcga
     8941 acgtatggag attatccgcg acttgcgtct gggtgagttc gacgtgctgg tagggatcaa
     9001 cttactgcgc gaaggtctgg atatgccgga agtgtcgctg gtggcgatcc tcgacgctga
     9061 caaagaaggc ttcctgcgtt ccgaacgttc gttgatccag actattggtc gtgcggcacg
     9121 taacgttaac ggtaaagcga ttctctacgg cgataagatc accccatcaa tggcgaaagc
     9181 gattggcgaa accgaacgtc gccgtgagaa acagcagaag tacaacgagg aacacggaat
     9241 tacgccgcaa ggcttgaaca agaaagtggt cgatatcctg gcgctggggc agaacattgc
     9301 caaaaccaaa gcgaagggca gaggaaaatc gcgcccgatt gttgagccgg ataatgtgcc
     9361 gatggatatg tcgcctaaag cgttgcaaca gaaaatccat gagctggaag ggttaatgat
     9421 gcaacacgcg cagaatctgg agttcgaaga agcggcacaa attcgtgacc agttgcatca
     9481 gctgcgtgag ctgtttatcg cggcttcgtg acaggataac gaagaagaca gatgacaaaa
     9541 gcaaaattgc ctgatgtgct acgcttatca ggcctacatg atctctgcaa tacattgaat
     9601 tagcgcggtt tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgcgcac
     9661 tttgtcttca ataggaagcc ggaatttttc ttcggatttc cgttaaccta aagcctgtaa
     9721 cgccttttcc agcgcgttat gtaacaactg gcggtcatga cgatacggaa tatcgctggc
     9781 ctccagtacc tcctgaatca caatccgctc tttcaccgcc gagacatcca cttttggccc
     9841 gacgatgacc gcatcaatga cctttttacc aacatactgc tccataattg ccagcttgct
     9901 ttccagcttc aaattagccg caggtaaact caactcacgc cccagattgc cgatatagac
     9961 catcggcgct ggcgtgcggc gtaatgcctg ggcgatttcc ttcagcagca gaattggcat
    10021 caggctggta taaaaactgc caggcccaat aatgatgaga tccgcttcat tgatagcgtg
    10081 aaccgcctca cgcgttgcgg gtacattcgg cgttaataat aactcttgaa tcggcgtagt
    10141 taactggtcg atattgacct cgccgtaaac ttcatgcccc tgatcgtcaa tcgccatcag
    10201 atcgacggga tgctctgaca ttggaatcaa atgcgtatcc actttcagca gattacgaat
    10261 taaattgatg gcttccagag gccgcacgct aaggtgatcc agcgccttta acatcaagtt
    10321 tccgagatta tgaccggaaa gttcgccatt gccgccaaaa cggtattcaa acatcgcgga
    10381 ggcgacgctc ggttccgtta tcagttggtt gaggcagttg cgcatatcgc cccaggcaat
    10441 gccgccttct gaacggcgaa tacgccccgt cgagccacca ttatccgtgg tggtgacgat
    10501 acccgttaaa cgagaaccca aagacgaaag tgatgagaga acgcgtccca gtccatgccc
    10561 tccgccgaga gcaacgacac gatcaagatc agccagcgta cgattgcgca tatagtttcc
    10621 tggagtcaga ttatccgcgc tacagtagcg caaattgcgg ggaaacagca attaacctgc
    10681 caatattgag gatacaaact attttcttct acctctaaag gacgatgcac gctatgcctc
    10741 cctgatgatg tatatcaaag taaaactgcc attttccctt attctgtagc gaaatagcac
    10801 gatcatgacg ctatatacat gattacatag cgaaagtgtg gatggtaaaa atctcattta
    10861 cacgctagta tcggcttaac cactaaacac tctagcctct gcacctgggt caactgatac
    10921 ggtgctttgg ccgtgacaat gctcgtaaag attgccacca gggcgaagga agaaatgact
    10981 tcgcctcccg tatctggaaa ggtgtacatg gcttcacaac tgactgatgc atttgcgcgt
    11041 aagttttact acttgcgcct gtcgattacc gatgtgtgta actttcgttg cacctactgc
    11101 ctgccggatg gctacaaacc gagcggcgtc accaataaag gctttcttac cgtcgatgaa
    11161 attcgccggg ttacgcgcgc cttcgccagt ctgggcaccg aaaaagtgcg cctgacagga
    11221 ggagagccgt ctttacgccg cgactttacc gatatcatcg ccgctgtgcg ggaaaacgac
    11281 gctatccgcc agattgcggt caccaccaat ggttaccgtc tggaacgcga tgtggcgaac
    11341 tggcgcgatg cgggacttac tggcattaac gtcagtgtcg acagtctgga cgcccgccag
    11401 tttcacgcta ttaccgggca ggataaattc aaccaggtca tggcagggat cgatgctgca
    11461 tttgaggtcg gttttgagaa ggtcaaagtc aataccgtgc tgatgcgtga tgttaatcat
    11521 caccagctcg acacctttct gaactggatc cagcatcgcc ctatccagct gcgtttcatc
    11581 gaactgatgg aaacgggcga gggcagtgag ctcttccgta agcatcacat ctctggtcag
    11641 gttctgcgtg acgagctact gcgtcgcggc tggatccacc aattacgtca acgcagcgac
    11701 ggtcctgcgc aagtcttttg ccatccagat tacgccggag agattggcct tatcatgccg
    11761 tatgaaaaag acttctgcgc cacttgcaac cgcctgcgcg tttcctccat tggtaaactc
    11821 catctctgcc tgtttggtga aggcggcgtt aacctgcgcg atctgctgga agacgatacc
    11881 cagcaacagg cgctggaagc gcgtatttca gcggcgctgc gggagaagaa acagacccat
    11941 ttcctgcatc aaaacaacac cggtattacg caaaacttat cgtacattgg cggctaaaac
    12001 gtcaaaagga gagatcagat gagtcaggta agcactgaat ttatcccgac ccgtattgct
    12061 attcttacgg tttctaatcg tcgcggtgaa gaagacgata cctccggtca ctatttgcgc
    12121 gattcggcgc aagaagcggg ccatcacgtt gtcgataaag ccattgtgaa agaaaaccgc
    12181 tacgctattc gcgctcaggt atctgcgtgg atcgccagcg acgatgtaca agtggtgttg
    12241 attacggggg ggactggcct gacggaaggt gatcaggctc ccgaagcatt gctgccgttg
    12301 ttcgaccgtg aagttgaagg ttttggtgaa gtgttccgga tgttgtcgtt tgaagagatt
    12361 ggcacttcca cgttgcaatc tcgtgcggta gcgggcgtcg ccaacaaaac gctgattttc
    12421 gccatgccgg gttcgaccaa agcgtgccgt accgcatggg aaaatatcat cgcgccgcag
    12481 ctggatgccc gtacgcgtcc gtgtaatttc catccacatt tgaagaaata agtatgtcgc
    12541 aactgaccca tatcaacgcc gctggcgaag cgcacatggt ggatgtctcc gccaaagcgg
    12601 aaaccgtgcg tgaagcgcgg gcggaagcct ttgtcaccat gcgcagcgag acgctggcga
    12661 tgattattga tggtcgccac cacaaaggcg acgtatttgc cactgcgcgt attgtcggta
    12721 ttcaggcggc aaaacgcacc tgggatctga tcccgctgtg tcacccgctg atgctcagca
    12781 aagttgaagt caatttacag gccgagccgg agcacaatcg ggtgcgtata gaaaccttat
    12841 gccgcctgac cgggaaaacc ggtgtcgaaa tggaagcgtt aaccgcggcc tccgtggcgg
    12901 cgctgaccat ttatgacatg tgcaaagcgg tgcaaaaaga tatggtgatt ggtccggtac
    12961 gtttgctggc gaagagcggc ggcaagtcgg gtgactttaa ggtggaagcg gatgattaaa
    13021 gttctttttt tcgcccaggt gcgcgagttg gtgggaacag atgcaaccga agtggctgcg
    13081 gattacccaa ccgttgaagc gttacgccag cacctggctg cgcagagcga tcgctgggcg
    13141 ctggcgctgg aagatggcaa attgctggct gccgtcaacc agacgctggt gagttttgac
    13201 catccgctga ctgacggcga cgaagtagct ttcttcccgc cggtaaccgg aggttaagat
    13261 ggcagaaacc aaaattgttg tcggcccgca gccgttcagc gtaggagaag agtacccgtg
    13321 gctggctgag cgtgacgaag acggtgcagt ggtcacgttt accggtaagg tgcgcaacca
    13381 taacctgggt gacagcgtca aagcattaac cctcgaacac tatccgggga tgactgaaaa
    13441 agcactggca gaaatcgttg atgaagcgcg taaccgctgg ccgctggggc gcgtcacggt
    13501 gattcaccgc atcggggaat tatggccggg cgatgaaatc gtttttgtcg gtgtcaccag
    13561 tgcgcatcgc agcagtgcgt ttgaagccgg gcagtttatt atggattatc tcaaaacccg
    13621 cgcaccgttc tggaagcgcg aagccacgcc ggaaggcgac cgctgggttg aagcccggga
    13681 gagcgatcag caggcggcaa aacgctggta gttttttgtt agccggataa ggcaccgctg
    13741 cgtccggcaa tttcaccgct cagtacatgc gtttacgctg cgtcgatgca cagcctcatc
    13801 attttgcagt atccttaaga tattccttat atcttcagga gatcgtcatg gacagattcc
    13861 cacgttctga ttcaatcgta caaccccggg ctggcttgca aacttatatg gcgcaagtct
    13921 atggctggat gaccgttggc ttgttgctga ccgcatttgt tgcctggtat gcggctaatt
    13981 ccgcggccgt gatggagctg ttgttcacta accgtgtctt tttaatcggt ctgatcatcg
    14041 cgcaattagc attggttatt gtgttatcag cgatgattca aaagctgagc gcaggtgtaa
    14101 cgacgatgct ctttatgctt tattcggcgc tgacgggtct tacgctttcc agtatattca
    14161 ttgtctatac cgctgcttct atcgccagta ctttcgtcgt tactgccggg atgttcggcg
    14221 caatgagcct gtacggttac accacgaagc gcgatttaag tggctttggc aatatgctgt
    14281 ttatggcgtt aatcggcatt gtgctggcat cgctggtcaa cttctggctg aaaagcgaag
    14341 cattgatgtg ggcagttacc tacatcggcg tgattgtctt tgtcggattg acggcgtatg
    14401 acactcagaa attgaaaaat atgggtgagc agattgatac ccgcgacacg tcgaacctgc
    14461 gcaaatattc cattcttggc gcgttaacct tgtatctgga cttcatcaac ctgttcctga
    14521 tgttgttacg gatcttcggc aaccgccgtt aattcttttt caccatcact atcccggcca
    14581 tgtgtcggga tttttttctt actaaaaacg acacttaaca agtaccaggt ataaaatatg
    14641 ccttatgagc gagcggaatc gttttccgta tccataaata caaaagtatt ttgtatgcgt
    14701 ggttttaaat ttattgatgg tgaatattaa tattttttct aaggacaact aaaagaatgg
    14761 aatcatactc gcaaaacagc aataaattag attttcagca cgaggccagg atataaaacg
    14821 gtatatggct cattaccgct ttaggtttgg tggcaaccgc aggactagcc tggggagcta
    14881 agtatctcga aattacggca accaaatatg attcaccacc aatgtatgtc gctatagggt
    14941 tattattact ttgtatgtat ggcttaagta aggatatcaa caagataaat gccgccatcg
    15001 cgggcgtaat atatctgttt ttactctctt tggtggcgat tgtcgttgca agtttagttc
    15061 ctgtatatgc cattatcatc gtgttcagca ctgcgggcgc gatgttttta atcagtatgc
    15121 tggccggttt attatttaat gttgagcctg gttctcaccg ttttatcatt atgatgacgt
    15181 tgacagggtt ggccctggta atcatcgtga atgcggcatt aatgagtgaa cggcccattt
    15241 gggtaataag ttgcttaatg attgtgttat ggccaggcat tatctcgcat ggacgaaata
    15301 agctccttga actggcagga aaatgccata gtgaagagtt gtggagtccg gttcgttgcg
    15361 cttttacagg tgcattaaca ctctattact attttatcgg cttctttggg atacttgccg
    15421 cgatagctat aacgcttgtc tggcaaaggc atacgcgttt ttttcattag ccgtaaatgc
    15481 acatactaaa gaacttaact aaacttcaca tcgccgcttc atttttcgcc cgtagcccag
    15541 tacaaagaaa tagttcacgc tttgaccaag taacagccag ataatcgccc ccattaccat
    15601 ccagttaacg ctggaaatca gcatctgagc gagggcgaat ttccatgaag gcagcaccag
    15661 cttttgccct ttgatggtca tatggcggtg cttcgcgaaa gcgcaaaacc acaaataaac
    15721 cgcgataatc atcagtaagc caatgccgag aatacgcagc gtagtttgat cgacatacca
    15781 gtgatccggc aactccacca cgcctgcggt aaagataatc cctgccagta aaatatagcc
    15841 cagccagttg gtggtaatac tgagcgagaa aatccgcgta atagtgctac cgggcaaccc
    15901 cagacgagag tacaaacgat agcgcatacc aatgccgccg acccaggtac tgagcgtcag
    15961 gttgaaggcg tagcagataa acgacaccag catcacctgg cgcttcgcca gtttgtgacc
    16021 gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc agatagctga cgaccaccag
    16081 cgaccgcact aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc
    16141 agtccacttt tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg
    16201 tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc
    16261 cgtgttttca gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac
    16321 ctgcgccaga cgtggcgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa
    16381 aaagtggaac gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt
    16441 ttcatccacc tgctgacaat ctgcggcgat aatgccgttc agattatcgc gcagcgtctg
    16501 gttaaaatta cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg
    16561 atcgagatta ctggacccta ctgtcgccca gtgatcgtcc atcaatgcta ctttgccgtg
    16621 gagcgggcgg cggcggtact caaacacctg aacgccgcct ttaaccagat agttatacag
    16681 caagcgcgcg ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat
    16741 ccgcaccccg cggcgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa
    16801 gtaggcgttg gcgataatga cttcccgccg cgcctgagtg agcattttca gatagtggcg
    16861 ttctatatca tcgcggtgtt cttcgttatc gcgccagacc agcaatacct gcgcgtctcc
    16921 cggctggcgg ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg
    16981 tccaggcagg ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg
    17041 taccgcgtaa tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt
    17101 cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg
    17161 aaacacattg gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac
    17221 gccagctgcc gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc
    17281 cagcaagact tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg
    17341 tttgccgacg ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc
    17401 gccaatcgcc ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt
    17461 attgccttcg cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca
    17521 gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg
    17581 acgtagatcc tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt
    17641 ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct
    17701 ttttgccgcc agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca
    17761 ttcacccatt cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga
    17821 tgtacgcaca tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc
    17881 acgccgcgct tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga
    17941 taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag
    18001 gcaaaatcgc tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt
    18061 tccacatgca gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg
    18121 gcgctgacgg tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg
    18181 gtaaagcctt tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg
    18241 ggcataattt tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa
    18301 aaggtgcggt cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat
    18361 tcttcaggag agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt
    18421 gctggcagat tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta
    18481 agactctggt tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga
    18541 tgctggtgtt tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag
    18601 agctgaccac gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg
    18661 tactggaaaa caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg
    18721 tagtgtcttt gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac
    18781 gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata
    18841 tccagactcg catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg
    18901 ttaatccacg tcaggttttg cagccatact ggcatgtttt ccaccggaga aacgtaaccg
    18961 gaaaggagaa tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc
    19021 gagcagagtg atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc
    19081 gtaaagtaga acagcgccag cgatccggcg aaggggattt gatacgccca gataccaatc
    19141 gccagcacaa tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg
    19201 aagatctgcc aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt
    19261 tcgcgggcga cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc
    19321 agcgacggca ccacaaacca tttgtagtca agattcgggt tataccagtt gcgtaccacc
    19381 agctcgctgt tgttaggttt cggttttcct tccagcagct cctgctgata atttttgacg
    19441 atctgttgca ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc
    19501 aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc
    19561 accagtagta acgccttttg tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc
    19621 agcagcacat gggtaaaggc gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc
    19681 tcgccgttat cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg
    19741 aacgggaaca ggatcacctg aataagcaca ggtaaaatca gaatcgcgcg ggtttgtggt
    19801 tcgcgcagca acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc
    19861 ttctccctaa tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc
    19921 cgaagcgatc aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa
    19981 caggctttgc agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat
    20041 caccgcgggc atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcaggaa
    20101 cgcggcgttc agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag
    20161 cagccccatc cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaataagcag
    20221 cgaaccgcga tacggcacgc cgagaataaa cactgacacc agcatacaca gcagcatcgc
    20281 cagcatcccg agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat
    20341 ctccgtagag agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga
    20401 ggtgagaatc gcgccgatga ccgtcatgat gatggtcact gcaccgggga taatgaagtg
    20461 ctggctaatc gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa
    20521 agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgtg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 ccggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaaggccg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgt tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 c
//
LOCUS       DAETQN010000033        34268 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.033, whole genome shotgun sequence.
ACCESSION   DAETQN010000033 DAETQN010000000
VERSION     DAETQN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34268
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.033"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            580..717
                     /locus_tag="K0W68_001613"
     CDS             580..717
                     /locus_tag="K0W68_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073201.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1317..2096
                     /gene="ipaJ"
                     /locus_tag="K0W68_001614"
     CDS             1317..2096
                     /gene="ipaJ"
                     /locus_tag="K0W68_001614"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HBI3073202.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3042..3971)
                     /gene="virB"
                     /locus_tag="K0W68_001615"
     CDS             complement(3042..3971)
                     /gene="virB"
                     /locus_tag="K0W68_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HBI3073203.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4188..4424)
                     /locus_tag="K0W68_001616"
     CDS             complement(4188..4424)
                     /locus_tag="K0W68_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBI3073204.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4428..6329)
                     /gene="ipaA"
                     /locus_tag="K0W68_001617"
     CDS             complement(4428..6329)
                     /gene="ipaA"
                     /locus_tag="K0W68_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HBI3073205.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKESFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFFSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6338..7336)
                     /gene="ipaD"
                     /locus_tag="K0W68_001618"
     CDS             complement(6338..7336)
                     /gene="ipaD"
                     /locus_tag="K0W68_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HBI3073206.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKKKYEDKPLYPATNTV
                     SQKEADKWLTELGGTIGKVSKKNGGYVVNINMTPIDNMLKSLNNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7387..8478)
                     /gene="ipaC"
                     /locus_tag="K0W68_001619"
     CDS             complement(7387..8478)
                     /gene="ipaC"
                     /locus_tag="K0W68_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HBI3073207.1"
                     /translation="MEIQNTKSTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMDTSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSTASQIAGNIRA"
     gene            complement(8498..10240)
                     /gene="ipaB"
                     /locus_tag="K0W68_001620"
     CDS             complement(8498..10240)
                     /gene="ipaB"
                     /locus_tag="K0W68_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HBI3073208.1"
                     /translation="MHNVSTTTTGLSLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANKNINTTNSHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPDSPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10246..10713)
                     /gene="ipgC"
                     /locus_tag="K0W68_001621"
     CDS             complement(10246..10713)
                     /gene="ipgC"
                     /locus_tag="K0W68_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HBI3073209.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10771..11397)
                     /gene="ipgB1"
                     /locus_tag="K0W68_001622"
     CDS             complement(10771..11397)
                     /gene="ipgB1"
                     /locus_tag="K0W68_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HBI3073210.1"
                     /translation="MQILNKILPQVEFAIPRPSFNSLSYNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDNVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSNMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11413..11802)
                     /gene="ipgA"
                     /locus_tag="K0W68_001623"
     CDS             complement(11413..11802)
                     /gene="ipgA"
                     /locus_tag="K0W68_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HBI3073211.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11815..13299)
                     /gene="icsB"
                     /locus_tag="K0W68_001624"
     CDS             complement(11815..13299)
                     /gene="icsB"
                     /locus_tag="K0W68_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HBI3073212.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEE
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQVNKLLLNNSHSNI"
     gene            13613..15229
                     /locus_tag="K0W68_001625"
     CDS             13613..15229
                     /locus_tag="K0W68_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HBI3073213.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVNKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDSKIWNMVKGYSSFV"
     gene            15239..15601
                     /gene="ipgE"
                     /locus_tag="K0W68_001626"
     CDS             15239..15601
                     /gene="ipgE"
                     /locus_tag="K0W68_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HBI3073214.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15601..16059
                     /gene="ipgF"
                     /locus_tag="K0W68_001627"
     CDS             15601..16059
                     /gene="ipgF"
                     /locus_tag="K0W68_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HBI3073215.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16068..17183
                     /gene="mxiG"
                     /locus_tag="K0W68_001628"
     CDS             16068..17183
                     /gene="mxiG"
                     /locus_tag="K0W68_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HBI3073216.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17191..17442
                     /gene="mxiH"
                     /locus_tag="K0W68_001629"
     CDS             17191..17442
                     /gene="mxiH"
                     /locus_tag="K0W68_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HBI3073217.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17455..17748
                     /gene="mxiI"
                     /locus_tag="K0W68_001630"
     CDS             17455..17748
                     /gene="mxiI"
                     /locus_tag="K0W68_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HBI3073218.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17754..18479
                     /gene="mxiJ"
                     /locus_tag="K0W68_001631"
     CDS             17754..18479
                     /gene="mxiJ"
                     /locus_tag="K0W68_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HBI3073219.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18476..19003
                     /locus_tag="K0W68_001632"
     CDS             18476..19003
                     /locus_tag="K0W68_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HBI3073220.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18945..19640
                     /gene="mxiN"
                     /locus_tag="K0W68_001633"
     CDS             18945..19640
                     /gene="mxiN"
                     /locus_tag="K0W68_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HBI3073221.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19633..20040
                     /gene="mxiM"
                     /locus_tag="K0W68_001634"
     CDS             19633..20040
                     /gene="mxiM"
                     /locus_tag="K0W68_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HBI3073222.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20024..20452
                     /gene="mxiM"
                     /locus_tag="K0W68_001635"
     CDS             20024..20452
                     /gene="mxiM"
                     /locus_tag="K0W68_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HBI3073223.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20582..21214
                     /gene="mxiE"
                     /locus_tag="K0W68_001636"
     CDS             20582..21214
                     /gene="mxiE"
                     /locus_tag="K0W68_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HBI3073224.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21201..22901
                     /gene="mxiD"
                     /locus_tag="K0W68_001637"
     CDS             21201..22901
                     /gene="mxiD"
                     /locus_tag="K0W68_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HBI3073225.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFDRFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22920..23987
                     /gene="mxiC"
                     /locus_tag="K0W68_001638"
     CDS             22920..23987
                     /gene="mxiC"
                     /locus_tag="K0W68_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HBI3073226.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24000..26060
                     /gene="mxiA"
                     /locus_tag="K0W68_001639"
                     /gene_synonym="virH"
     CDS             24000..26060
                     /gene="mxiA"
                     /locus_tag="K0W68_001639"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HBI3073227.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26073..26474
                     /gene="spa15"
                     /locus_tag="K0W68_001640"
                     /gene_synonym="spaK"
     CDS             26073..26474
                     /gene="spa15"
                     /locus_tag="K0W68_001640"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HBI3073228.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26478..27770
                     /gene="spaL"
                     /locus_tag="K0W68_001641"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26478..27770
                     /gene="spaL"
                     /locus_tag="K0W68_001641"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HBI3073229.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKNRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27863..28201
                     /gene="spa13"
                     /locus_tag="K0W68_001642"
                     /gene_synonym="spaM"
     CDS             27863..28201
                     /gene="spa13"
                     /locus_tag="K0W68_001642"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HBI3073230.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28188..29066
                     /gene="spa32"
                     /locus_tag="K0W68_001643"
     CDS             28188..29066
                     /gene="spa32"
                     /locus_tag="K0W68_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HBI3073231.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29060..29941
                     /gene="spa33"
                     /locus_tag="K0W68_001644"
                     /gene_synonym="spaO"
     CDS             29060..29941
                     /gene="spa33"
                     /locus_tag="K0W68_001644"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HBI3073232.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29946..30596
                     /gene="spa24"
                     /locus_tag="K0W68_001645"
                     /gene_synonym="spaP"
     CDS             29946..30596
                     /gene="spa24"
                     /locus_tag="K0W68_001645"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HBI3073233.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            30611..30871
                     /gene="spaQ"
                     /locus_tag="K0W68_001646"
     CDS             30611..30871
                     /gene="spaQ"
                     /locus_tag="K0W68_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HBI3073234.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30871..31641
                     /gene="spa29"
                     /locus_tag="K0W68_001647"
                     /gene_synonym="spaR"
     CDS             30871..31641
                     /gene="spa29"
                     /locus_tag="K0W68_001647"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HBI3073235.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31648..32676
                     /gene="spa40"
                     /locus_tag="K0W68_001648"
                     /gene_synonym="spaS"
     CDS             31648..32676
                     /gene="spa40"
                     /locus_tag="K0W68_001648"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HBI3073236.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32689..32931
                     /locus_tag="K0W68_001649"
     CDS             32689..32931
                     /locus_tag="K0W68_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073237.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
     gene            complement(33538..34268)
                     /locus_tag="K0W68_001650"
                     /pseudo
     CDS             complement(33538..34268)
                     /locus_tag="K0W68_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        10625 a         5065 c         6894 g        11684 t
ORIGIN      
        1 gatctgattt caggcgttgg gtgtggatca ctattgcacc gttcgtgaca cttgttgact
       61 gcctgtgcgg catctggtat caaggtggcg tgaagccggt tcaggttctc atgcggaacg
      121 ttggaaccta tatccccccg gtaccaaagg cgaaatgtaa gtgacaaaat gcaatgaaca
      181 cgttctccgt taactgagga gcatggtgat aaataaaggt aaatgcagaa ggtgtagagt
      241 ctcgcagatt tctgacatca tagagcctaa cattagatgc ataacgctac agattgtaga
      301 atctctacag gtgaaatata cactggttgg cgtttatgtc gcaaaacatg tcattcaggt
      361 tcatctcacc aataaggata tgagtgaagt ggaggataag tgagtctgct gtcagagttt
      421 tctggtgtat gtcagtaact gcgaaccatg tctgcttgat atgggagctt atggaggctc
      481 tctgtacaag gttagggagc taacaaagca gggataaaaa gtccgcctga tgcaggcacg
      541 gttcgttaaa tcttttgcca ttggtaacaa aatggtgcga tgaatgttca ggctatcttt
      601 atcttgatgg tggttcagct gtttggtaaa gaaatcgctg taacaataga agaactgcag
      661 gcagtactgg tcccacatcg tttgctcagt caactggtga agttccgtag cgcgtaaatt
      721 aatgctttgc atgaaacgtt actggagtgg atgaaagaat acctgcatat atcattactg
      781 cgttggatta ccagtacaac agattgaatg aactggacat gttgatagac ggcattgaaa
      841 agcagcttat aagcatagtg cggcagagtt aattggagag cacttcgcct tctgagtatt
      901 aagtaagcat ggtggtacct gcggatacta tctattcact gtgctcggtc ggcagcctta
      961 ctgaccaagg agttggggga gtcgataaca aagttgaaaa aacagctctc aaccaaggtg
     1021 gttattgtaa caatagcaaa taagctggtg tgacctgaat cacaataacc gattatggac
     1081 gtaaatacga caaaaatatg tcagtatcag gcggtattaa ttgctgatgc tgatggacat
     1141 taactcttaa taaaagggtg gatgctgaaa ggttgttatg gtgacggacg tgatgacgac
     1201 ggaaaagagt atgaggtttt ggatttaaag ttcgttcggg gaataaggac ccggtcagct
     1261 aattctcata gtagttcgca ttgctggatg caatactgtg ctgcaattac tcgtcgatgt
     1321 cggaacaacg gaagccttgc aaacggggat gtattcatac aggtgttatg ttatatgggg
     1381 ttttattgca gggggctata ccaagagaat atatgatatc tcatcagact gatgtgaggg
     1441 taaatgagaa tagagtaaac gaacaaggtt gttttttggc gaggaagcag atgtatgaca
     1501 attcttgtgg agctgccagt ttactctgtg ctgcaaagga attaggggtt gataaaatac
     1561 cacaatacaa agggtctatg tccgaaatga caaggaagag ttctcttgat ttggataatc
     1621 gttgtgagcg ggatttatac ttaataacta gtggtaatta taatccgaga atacacaaag
     1681 ataatatcgc ggatgccggt tattctatgc ctgataagat agtcatggct acgaggcttc
     1741 ttggattgaa tgcttatgtc gttgaagaaa gtaacatttt ttcgcaagta attagtttta
     1801 tttatcccga tgcaagggac ttattaattg gcatgggatg taatatagta catcaacgag
     1861 atgtgttatc ttctaaccaa agagttttgg aagctgttgc tgtatccttt attggagttc
     1921 ctgtcggcct ccactgggtg ttgtgtagac ctgatggaag ctatatggat ccagctgtag
     1981 gagaaaatta ttcttgtttt tcaactatgg aattaggtgc aagaagaagt aattctaact
     2041 ttataggata tactaaaata ggtatttcca tagttataac taatgaggct ttgtaaattg
     2101 tcgtttttta tctgtgaagg agcttgaata ttttgggggc ggggggcata acaaatgata
     2161 ttactgagta caaatttagc tatattgggc ggattagttg tgtgggaaga cgaaatgttt
     2221 ctcttgaaat aagtgaactc cctcaaggaa tgattgtgtg cttaaatatt taagtgctgg
     2281 taagtttgag aaggctgaca tattttgcag agcaaacagt gattgacttg gatgatattt
     2341 ttattttagt cactcgttgt aattggagta tgctttattt tatttgaaaa tagatgtatt
     2401 ttatacccgc aatttaaact gtaagcccta tgaggggtat ggatattata agttactctt
     2461 tgactaagca taacttttgg ggttagaaaa ataaatctct tgttaataat taatatgatt
     2521 ttaaaatcaa acttgtttat aattataaaa taatgtaatt cggtcatttt atgggaagat
     2581 taggagaata aaacagtaat tatatttgag aaatctgcta atatattctt aaagcttttt
     2641 tgatactgtg aacgaagtct gttaatggtg gattttttgt ttatcaccat gtaaaaaaat
     2701 tgagtagtaa aagcgttgac tgctaatcct cgctgttttt attgaaacaa ttaaaaatac
     2761 gctgaatatt ttcggccagt cactcatctt cacataaaat tttgaccatc atgccgcatc
     2821 ccttagttga caattcattt tcaatgatat gaatattact aatttcacca gataatgttc
     2881 tcgtggtcag cagggtatga gttttcaccg taattttaag gaaacacagt gcgtggggat
     2941 tgaataacag atagccgacg gaattacccc accggctgaa agtcaagttc aacgaagatt
     3001 tttattatct gaattgggca gtttacatca gtgttcgatg tttatgaaga cgatagatgg
     3061 cgagaaatta tatcccgaat agcttcatct atttctctct gaactgtttt gtttatttta
     3121 gataatgtaa atattagagt tctgtttgtt ttattctctt ttctttttat gtaagtccgt
     3181 ttgtctttag aaataaataa cgagtcaact tttagcgaag ggttttgctt tctgtttttg
     3241 cttttcttta tgatgtttaa aatttctttt ttatatatgt ccgggggcaa atttgtatca
     3301 aggtctttta tttcttcctt taatattggt agtgtagaac taagagattc attagccttt
     3361 tcaagtcctt tataataatt gaataatatc ttgtagtcat taaagttaag ttctgacgcg
     3421 attggaaata gagatattat ttcctgtgga acgcttgctg cctgaaaggc acgagtgact
     3481 ttcgcgcgag acagattctc ttttttggct atgtctttat aggacatccc tgatactttc
     3541 agaaaattaa gaccgatacc aagttctcgg atgctatgct cttttgctgt ttgtatatcg
     3601 tttgctagtt ttctggcatc aagagtagat atatattctt ttgaatatag aacttcaaga
     3661 tctgctcctg catatattgc agatgctctt ctacgagtgc catccagaat ttcaattcta
     3721 ccatcaatct cccttcctat tacagggaag aattgttgta gctttatagt ttttatgata
     3781 tcggctaatg attcttctgt tagggattct tgatcgcgta cattcgtttt ttggttgacg
     3841 aaggttaaat cttggatcct tttatgagga atcttggctt tgataaagga aactttatta
     3901 ccagaatgga gtgtaaactc tttcttttgg ccttccttta tacttaacaa gtcgttgcac
     3961 aaatccacca tatcacaccc tgtttattca tattgattta tattgacaaa agttaaatgc
     4021 agtgtgagca agattgtatg taactgttga tgatagtcaa cggtagtctt aaaaattact
     4081 attttaatag ctggattgat gcaatttatc tttattggat aaaagaataa tattcttgtg
     4141 gagctctgat gtgagagctc catagatgaa aaaatgctta tcaattacta tattgatttt
     4201 gtactatttt tgactacaga aataagatct ccaatagtaa taatgtgttc taatgcagat
     4261 tcatctatac gtagattgaa ctcaaaacta atcatatcca gcatttcaat cagagaaaga
     4321 gaatcggcag aaagatcttc aataaattta gtttcttcag atagtttgct gtacgctata
     4381 ccatagcaaa aggccactat tgataatatt ttttctttta tcatatttta atccttattg
     4441 atattcttta atacttttga tagggaagtg gttacatctt ttgctgcttt atatatatta
     4501 ttgttttcag ctgtaatatc attgttattt tttaaatcag atatatcatc tgtaagtaat
     4561 tctgcagagg tatcgtcaat ttttgacaat accttgctga gggcgctaga tacttctttt
     4621 gccttttcat agatcgcatg atttttatca attgtatctg cgttggtttc tgttacgtag
     4681 tttattgtat ttgcatctgt aacttttaat ttgttgttta ttctgtgttc tgttgtggat
     4741 tttgtaatgt cctttgattt ttcctgtaac tctcgttctt gtgttcctga tttagttcca
     4801 atgagagata taagattact tagggagttt gttacttttt ttgacgcttc aaatatcgtt
     4861 tcccgtgtta aatatgtgtc tccatttctt gagaaaaaat cagtctgaag tacttttgaa
     4921 tcagtgtttg agttgaattt attgatttgc gaagctactc tatgagaaag agaagcgcta
     4981 cttttagttg tatctgatga gttatcatta ctgcttgtca ctccttccgg gtggagaggg
     5041 ggggtggttg aagagttctg tatgttcaga ggcctgtgtc cccgagaaag agagttattt
     5101 gcagatcttt cttgactact cgtagttaac aggtctgaac ctattccatc cgaattatct
     5161 ggagtatggt tgaaaacctt ttctaaagct gaattatttg caaatgtctg gacagtatct
     5221 gatatagagt gtggtagaac ggaagttact ttttgaacta gtgctctttg tggatttagt
     5281 aatgagtttc cgttacggga taatagatcg tttgtctgag aagtaggaga ctgtgcatca
     5341 ttttctgggt tctcgtgata gctattgtca tatactctgt tgtcaaaaac tctattatcg
     5401 taagttctat tatcattatt tatatgatag tgaattacag gttgggatgt tttctcattg
     5461 gctccggggg ttggtccgcc atctggcata tcttcgggag gtgtagggag gccatcttgg
     5521 ataggatgcg aatcttttat atcttctaaa atatcattaa ggacttttct caacagctca
     5581 ggaaacacgg ctactgtatt taagtctctt gacagtttac ctataccaaa tcctttacta
     5641 tcaatagtat ttttacgttg attgcgcaaa ctatcaaagg caagttttgc ctgtgcgtca
     5701 actaaatcat ctagtttgag agatgaaaca gtttcattac ttaactttaa ttcagcaaga
     5761 atattattct tgataaatga tgatacttca gtattgagga gtttttgtcc caatgataaa
     5821 gcttcaccag atgtgtgttg ttgtgctaat agtgcaagtc tgtgctcaat gttttgtcgt
     5881 aattgatgga gtttcttatt ctttgccgac catggtccaa aggcagaatc ttgggataat
     5941 ttatctttta taattagttc cgaatactgt gcgacaattt tttgaagtcc cttttcgttt
     6001 tcaaaattaa gaccattttg atgtactaag gagtacaaaa catttgaaaa ttcgagaatc
     6061 tcgcttttgg catacgcatt cattgagttg gaataaatgc ttcttaacac atcttctttc
     6121 cctgaggaag aaaataaaaa atcaagacat ttctgattaa aagacgttgc aaagctttct
     6181 ttttcagaca ctgatgctgg tgtttttaga gaagtttgcg aactatggat atcggatatt
     6241 ttgcttttta actcgctgta ttctgttgat gatggtgaag ttgccttata taagaatgtt
     6301 ggcgcttgag tattatttac attatgcatg gcgcacctca gaaatggaga aaaagtttat
     6361 ctgtatctgt acatgagctt attgtactac tcaaaacctt tactaaatta tcaaaaatac
     6421 tattggcatt actgtatttt tgaactaaag tttgaagatt atttttcatt gtttcatctt
     6481 cggcagagaa tccggcattc catgcctgat attttgcatt atctagcaca acctcgccat
     6541 ttccacctag attatttaag ctttttaaca tattgtctat tggggtcatg tttatattga
     6601 caacatatcc cccgtttttt ttagatacct tgccgattgt tccacctaat tctgtaagcc
     6661 atttatctgc ttctttctga ctaacagtat tagttgctgg atatagcggt ttatcttcat
     6721 attttttctt gagttcttcc aatgcttttt taagcgagtt gacttgtaat ttcacggagt
     6781 ttccgtcgtt acctccggga gagatccagc cggcaagact ggaaagaaca gcgctaaaat
     6841 cttgatacat ttgagtatat gaactaacgg catgttcata tactttcaga tactgttcat
     6901 taatatcatt gatggagttt gcaattttag cccacagttc tctatgagat atcatttgat
     6961 ctccatcaag ctcggcttct ttcggggctg aatgtaataa ttctcttgcg tctttattaa
     7021 ttggatattc gttcctggaa agaatatcca atagttgagc ggatttattt acatccatgc
     7081 taatctgaga tgaatgtaat gctatttctt ctagcgatgt tttagtcaac gttttttgtg
     7141 aaagctcttt ctttaatgcc tgatttgttg ttctgatatt atgaagggtg tcgttgagca
     7201 tagtaaggga acttacagga tgagaactgg tcgttgtttt tatatcagaa ttaactgttt
     7261 cggttgatga accgttggta ttgtttggac tgaatgatga ggtggaaata ctattagtca
     7321 gagttgttat attcatggtt atttccttac aggaagagcc atatattatt atggctcttg
     7381 gatatattaa gctcgaatgt taccagcaat ctgactggct gtcgaattcc ttgattgggt
     7441 gatgctgtca attatattca ataatttttg tattaattga tttgtcgctt gggatgcttc
     7501 tttagatact tgggatgctt cctctgcttg tttactgctg gcctgactga ttagttgttc
     7561 ttcttcttca agagcagatg cataacgccc tcctgatgtg gatatattac cagcaacggc
     7621 tgatgtgtcc attgttgcac ggccaatatt ttgtttttgc tgcgcagaaa gggtattgag
     7681 ctcgtaagtt cttctctggg tgtcaatttt atcctgcaaa gaaatatcgg gagaactaag
     7741 agaagtttta tgttcagttg acagggatat attatctggc gccagtttat ttttacctaa
     7801 aaattttgtg ctagagttag tttgtggtga ggtgatattt gtatctattt gtttatttaa
     7861 ccctaattta gaacctgcaa gctctttttc aagagattga gcagtggcaa ggttctttct
     7921 taaggctcct ttttggtcgc taatccctga atgcgttttt ttggcaccga tacccgttat
     7981 acctacttgt gtgactgctc cagtaatgct tgatgatagg gctgccaggc cttgccgaac
     8041 aatgttctct gcagctgatt ttgtagcatc gaacgcaatc aatgacaatt gagagcccaa
     8101 tttagtttct gcagtgcgga gagcagaaag tagaacggct acactaataa ttaaagaaac
     8161 agcattagaa gaaagactgg aaatatccag agtgttctct ggagtaaggg tgtgactcat
     8221 ctctgaaaga tctttcatct ttttgtcagt aaggcgagca atgatttctg aatcatgctg
     8281 aacctgctct gataacttta aaagttgatc atcatttaat gtggttgtta atacgggatt
     8341 tttaccgaca ttaagtggaa tatgcgctgc aatctgctga taattttgtg atttttgtgt
     8401 ttcggaagaa ctttgagttt gttttgtgga tatatctgta tataaaatct gggttgattt
     8461 tgtgttttga atttccataa cattctcctt atttgtatca agcagtagtt tgttgcaaaa
     8521 ttgcttttgc aacatcagtt ctattagcct gagagttgga cattgaggct aatagatctg
     8581 caattacttc ctgcaattgg ccgaattttt ctattgcttc actgatatat tttgacagtt
     8641 gttcaacttg atatttcgat aatgtcaggt ctgctagatt tgtagacgcg ctgttctgga
     8701 aaacagcaga agcgacactt cctcccgctt gtgtggcaga gttaacactt tctcctaata
     8761 aaactgcttg gtttaaatgg gtggaaataa tttgtttgga tattacttca tcacccgctg
     8821 caccaagaaa tttatttaat ctggcaacag cattagtgat taaatcgtcc agttgagaag
     8881 aaaaattctt gagaaacttt ggtataaggt ctgtgagggt tttacctatt attttgccaa
     8941 tattctctgc aagttttgct gctgcctgtt taccaacagt ggctacgaga actactgctg
     9001 caaccaggac aagagcgcct gcgattgccc ccagaataga gccaatcatt ttggcttttt
     9061 tcgagtcgac gcccaagcct tcgagcattt ttgtaaatgc atctgaaagg agtttgatta
     9121 agggttcaat gactgctttc atgatcggat tcagggcttg ttccatgaag gaattgccgg
     9181 tcgctgcttg tactatagca tccgtaacca taagagctaa accaacagct gccagtgcta
     9241 gagaggctcc tccagaaaaa gctgctgcaa caacactaac gatagttaaa agtgccccaa
     9301 gtattttccc aacacaaccc attactctgt tgagttcttc tgctttacgt acttcagcag
     9361 catactcatc agattttctc tccatttcag tttttcttga ttcttggaga gactggaata
     9421 gagccagatc attttttaaa gattcttcat tattttttcc aactagttga ataaaggttg
     9481 ccatcaattg agtaacactg gcaagtccgg ttaatgattt ctgctgggtt gatagctgtt
     9541 cagcagatgc tgtgtttgaa aatgcagaaa aagagtctat ttctttttcg agttgcattg
     9601 atttatctgt aagtttgcta tgaattgaca gggttttctg ctcaatcaat gtcctgtctt
     9661 taactgctgc gtcttttttg atagtgagtt gtatttcttc ccggcttaat tttttctttt
     9721 ctggtgaatc tgggtcgagt tcggataatc ttgtttgaat ttggttaatt ttattttcta
     9781 ggtcttttat tttagaatct gcgtttttta gtttattaat ttgtttttca tagtctctgg
     9841 ttagtccttc agtttcagat agaagagtat taattttatc ggagaattct aggttttttt
     9901 gctgtcttgc ctgttgctga gacttccaag cagtaatttt atttgttaat gcagttaaag
     9961 atttttcacc gagtatttga ataaggtttc caattaaaag cgttagttgg gaacttgcat
    10021 ttaatgactt tggtgcttta agttcaggga ttaatatatt tgaagttgag tgtgaattag
    10081 ttgtattaat atttttgtta gcagtaagat cagcaattgt aagagaaaat aatttgttag
    10141 ctgcatcatt tgcagcctgg atagtattgt ctccaagctc agtggaagcc aatattttgg
    10201 caagagacaa accagtggtt gtggtactta cattatgcat aataattact ccttgatatc
    10261 ctgaattgcg tccaagtatg attgtgcttt tatttttaat ttttcatcat tgctgtgttg
    10321 aattacgagt tcgaagcact ctttggcttt caagggggct ttcaaccgaa gctggcattg
    10381 tccagtatgg aataccggtg tatagtcatt ttttcctaat gcaaaagcga cagcataaag
    10441 gtctgctgct tgttggaact gttcttttat ctgataaata gctgcgagtc ccataatgta
    10501 gtctacattg taaaagtcgt atatacataa aaatctgaag aaaacttcag cttcctctat
    10561 tcttcctttg ttgtaaaagt cataagcata tgaataaatg tcatccatca tatcatcagg
    10621 aattgcatta atatctttca gtgtagcgcc agagttaatt gcatcaatta ctgcagtaga
    10681 gatgctttca ttttcagtga tatttaaaga cataaggtct cctttttgtc tgctttactt
    10741 taataagtat aagatttaat ataaacagat ttaatttgta ttgctttgac ggtatacggc
    10801 ctttagaacc tctgcgctta tttctatttg taaaactttt gttgattcat taaatatttt
    10861 atcgtagacg gaactggata ctagatttgt aagatccttc atttgtgtgt taaacaaatc
    10921 catgttactg ctatagcgtt ttaaaatctt tgctctgagg ccagatgagg caatctcgtt
    10981 tagtgctgta tgactgatag atgtttgtgc tgatttttca tcaagtttta tatccgggaa
    11041 ttttttttca atagctgcaa atatgttttt tttgttatct gaggtcaaaa caactccatt
    11101 gtgtatggcc atttcatcta tacttctgtt tatcattgaa gagacataag aggttcgctc
    11161 ttggctcatc caacaaaata gttgattccc cgaattacta tcttttattt tgtctatgac
    11221 gttgtccctg attttattta tattcaccgg gcatccaaaa ttcttctgag gaaagcgttg
    11281 ttttaagtta aaaacagata atattttctt tacaagctta ttgtaactta acgaattaaa
    11341 tgatgggcga ggaatcgcga attcaacctg tggaagtatt ttgtttagaa tttgcatgat
    11401 accccctata tgttagttca cttctgaagt gatgtttgca atggttagtt taatacaaaa
    11461 taaaaattca tacaaagatt caatgagttc atcaggtgaa agatattttt tgtagtgttt
    11521 cttggtcaaa acacattgat gagtatcagg gagaagacag atagaagaac cataatctct
    11581 actcaaagag tagttaagga tgagtatgtg ttctggatta ataatatttt ctgtaaaaag
    11641 ttcatgttca ttaagaagtc cggtcaaaaa gatgtattca tcattatctg ttatacaaac
    11701 aggtatttgt tcttcgagca atataaaggc ttgattttta tcgttaaaat caagctttgc
    11761 acctgttatt tcataaagtt tatcatatag tttgcgacac atttctatgg ccttctatat
    11821 attagaatga gagttattca ataaaagctt attgacctgt aataagtcgt gaatgtaaag
    11881 tttggttcca agatctggcg atttaagaga attgtaataa tcaatagttt ttattgactt
    11941 ttcgataatt tcttcttttt tatctgttgc tccggttttt aattcgttta atacacgaga
    12001 catattgctg ggtgctgatt ttactaagta tgagtggcaa gcgttgatga ccgcatcaaa
    12061 gtcttctgaa gatacatttt cagtaggatg tttgtaaagt gaaattgata aaggcttgaa
    12121 tgtttttggg gtgtggataa ggttaatttt attgaaactt ttaagatagc tacgtcgtaa
    12181 ttgagtcaat ctggaaagat caaaatcaga ttcaatatac ttcattatct cgttataaga
    12241 ctgattgaga ctttcaatgc gttgcctaac tttatttgca tacgcgtata cattgttagg
    12301 tgtagcaacc aatttaacgt cagggagtgg aaagtatatt tcagaatttc ctgctttaag
    12361 aacattaagt gccataccag cacagttttc agatttactg attaatttat aatttgccaa
    12421 ttgtctgaaa gcatctgctt tcttttggca tataaatttt gacatgtttg tttcatcaag
    12481 gccaaaaagg ttatagctga ttttaccatc ctttgtttta ttatcacctg ataatggaat
    12541 gtatacttta tcagcactga ccccccaata accatagggg tctttttttt gattagctct
    12601 gggaaaaaat atttttttgt tgttattttg atcttgtttt gaatttaata agtttctaac
    12661 ctcttggcct gcatcaagtc tttcggctgt ttggtctgaa atcatattta gtttgtcttg
    12721 tttataggta tcgacactgt aacctgagcg tttttcaaaa aggtagttta tttctgtatc
    12781 ttttgtaaca ttcccgccgg gataccagct gagataggta tgagttggtt cctgtagtaa
    12841 attgtttttt atactgatac tggtatgccc taaatgggtt atgcctctat ttccccagca
    12901 acgaaaaact aaatgtcttt caagattttt ggctgtaccg atgccatgaa aacgctctgc
    12961 ggtattcaac attaatttga tgtctttacc tgtaaaagaa tgttgaattg aatttttgac
    13021 tgcttcatag gcggcaaacg cactttctgc agatttttcg ctagctaatg tcttgaggaa
    13081 aagcaataag gatttttgat tcgctagtcg ataatttttt agttgttcat ttaatatctg
    13141 tgaatttatc tttgcattaa tagtgcttaa tgttctgaaa aagagatcct ttaggttgaa
    13201 tttctgtgct accaatatag gtccatgctc tgtatcttca acgcgtatag gcccttttgt
    13261 attgcttgcg tcaatgaaat tgctaatttt gaggatcata ctttattaac tctccattac
    13321 gtggtgattg tggggatgta aaatgtattt gatatataat atattcaatt aataaattta
    13381 gaaacttgag cctgttaaca taatcaaatt ttcttttgtt gtacataata tgtacctcgt
    13441 gagcatatgt agtgctcgtt tcatcatgaa atcccacaag ttaaagtgtc tgatatatca
    13501 ggctcggagt gttatagaaa aagagagaac cctgttgaat aagtatcttt tttgttgtaa
    13561 aacgaggtac aaaactgcaa tcaatgcccc atttatgact gaggataatt aaatgcacat
    13621 aactaatttg ggattgcatc aggtttcatt tcaaagcgga gattcctata aaggcgcaga
    13681 agaaaccgga aagcacaaag gtgtaagcgt gatttcatat caaagagtta aaaatggaga
    13741 aagaaataag gggatcgaag cgcttaatcg attatattta cagaaccaaa caagcttaac
    13801 aggaaaaagc cttttgtttg cgcgtgacag agctgaagtt ttttacgagg caataaagtt
    13861 ggcaggtggt gatacgtcaa aaattaaagc catgatggaa cgattagata cctataaact
    13921 tggtgaagtt aataaaagac atattaatga gcttaataaa gtaataagtg aagagataag
    13981 agcacagcta ggcattaaaa ataaaaagga attgcaaacg aaaataaaac aaatatttac
    14041 tgattattta aataataaga attggggacc ggtcaacaag aatattagtc atcatgggaa
    14101 aaattatggt tttcaattaa cacctgcctc tcatatgaag atagggaata aaaatatatt
    14161 tgtcaaagag tataatggaa aaggaatttg ttgtgcttct acgagagaga gtgatcatat
    14221 cgcgaatatg tggctttcaa aagtggtgga tgacgagggg aaggaaattt tttcgggtat
    14281 cagacatgga gttatatcag cctatggatt gaaaaaaaac tcatctgaaa gagctgttgc
    14341 tgctcgtaat aaagctgagg agttagtaag cgcagcatta tatagcagac cagagttatt
    14401 atcacaggct ttgtctggta aaacagtaga tttaaagatt gtttcgactt ctcttctgac
    14461 gccgaccagt ttaaccgggg gagaggagag tatgttaaaa gatcaggtga acgcattaaa
    14521 agggttaaac tctaaaaggg gggagccaac gaaattatta attcggaata gtgatggtct
    14581 tctgaaagaa gtaagcgtta atctaaaagt ggtgacattt aactttggtg taaatgaatt
    14641 agcgctaaaa atgggcttag gctggaggaa tgttgacaag cttaatgatg aatcaatatg
    14701 ttctttgttg ggggataatt tcctcaaaaa tggtgtgatt ggaggttggg ctgcagaagc
    14761 aatagaaaaa aatccaccat gtaagaatga tgtgatatat ctggctaacc agataaaaga
    14821 gattataaac aagaagttac aaaaaaatga taatggagag ccgtataaat tgtcacaaag
    14881 aatgactcta ttggcttata ctataggtgc tgtaccttgc tggaattgta agagtgggaa
    14941 ggacagaaca ggtatgcagg atgctgaaat taaaagagaa ataataagaa aacatgaaac
    15001 aggtcagttt tctcaattaa atagtaaatt atcctcagaa gagaaaagat tattttctac
    15061 tattctaatg aatagtggta atatggaaat ccaagagatg aatactggtg tgcccggaaa
    15121 taaagtcatg aaaaaattgc cgctatcttc gttagagcta tcttattctg aaagaatagg
    15181 ggactcaaaa atatggaata tggtgaaagg gtattcgtca tttgtataag aggaatatat
    15241 ggaagattta gcagatgtta tttgccgagc cttgggtatc cctttaattg atatcgatga
    15301 tcaagcaatc atgcttgatg atgatgtgct aatatatatt gaaaaagaag gagattcaat
    15361 aaatttatta tgcccttttt gtgcattgcc tgaaaatata aatgatctta tatatgcgtt
    15421 gagcctaaat tactcagaaa agatatgctt agctactgat gatgaaggcg gaaatctaat
    15481 tgcgcgatta gatcttaccg ggataaatga attcgaggat gtatatgtta atacagaata
    15541 ttatatttcg cgtgtgcgtt ggctgaagga tgaatttgcg cgaagaatga aggggtatta
    15601 atgtcccgtt tcgtatttat tttgctgtgt tttatcccat atttagggag agctgattgt
    15661 tgggataagg ctggtgaaag gtacaatatt ccatcaagtt tgttaaaagc gattgcggaa
    15721 aaagaatccg gatttaataa atttgcagtc aatgttaata acaatggaag taaagattat
    15781 ggtataatgc aaataaatga ctttcattct aaaagactta gagaaatggg atattctgag
    15841 gaaatgctaa ttagtcatcc atgtctttct gtacattatg cagcaaagtt attaaatgag
    15901 tttatgatga tgtatggaag agggtgggag gctgttggag cctataatgc aggaacgtct
    15961 ccgaaaaaga aaaaagaaag attaaagtac gcagaggata tctatagacg ctatttgcgg
    16021 atagcagcag agagcaagca gaataatcga aggatataag aggatttatg tctgaggcaa
    16081 agaactcaaa tcttgcacca ttcaggttat tagtgaaact cacaaacggt gtcggggatg
    16141 agtttccgtt atattatggg aataacttaa ttgttctcgg acggacgata gaaacacttg
    16201 agtttggaaa cgacaatttc ccagaaaata ttattcctgt aacagatagt aaatcggatg
    16261 gtataattta tttgaccata agcaaagata atatctgtca atttagtgat gaaaaaggtg
    16321 agcaaattga tatcaattca caatttaatt catttgaata tgacgggatt tcttttcacc
    16381 ttaaaaatat gagggaggat aaatcgagag gccatatact aaacggaatg tataagaacc
    16441 actcggtgtt ttttttcttt gctgttattg ttgtgttaat tataattttt tccctctctt
    16501 taaagaaaga tgaagtaaaa gaaattgctg aaattataga tgataagaga tatggaatag
    16561 tcaacactgg acagtgcaat tatattcttg ccgaaacaca aaatgatgcg gtatgggcat
    16621 ctgtagcttt aaataaaacc ggatttacca aatgcagata tattctggtg tcaaacaaag
    16681 aaataaatag aattcaacaa tatatcaatc aacgattccc atttatcaat ttgtatgtgt
    16741 tgaatttagt gagtgataaa gcggaattac tggtgttttt aagtaaagaa agaaatagtt
    16801 caaaagatac cgaacttgat aaattgaaaa atgctctgat tgttgaattt ccgtatatca
    16861 aaaatattaa attcaactat cttagcgatc ataatgcaag gggggatgct aaaggtattt
    16921 tcacaaaggt aaatgtccag tataaagaga tttgtgaaaa taataaagtt acatattctg
    16981 tcagagaaga actgacagat gaaaagcttg agctcattaa tagactgatt tctgaacata
    17041 aaaatatata tggagatcag tatattgagt tttctgtact tttgatagat gatgatttta
    17101 aaggtaaatc atatcttaac agcaaagaca gttatgtaat gttgaatgat aaacactggt
    17161 tttttttaga taaaaataag tgaggataaa atgagtgtta cagtaccgaa tgatgattgg
    17221 acactgagtt cattatctga aacttttgat gatggaactc aaacattaca aggtgaacta
    17281 acattggcac tagataaatt agctaaaaat ccttcgaatc cacagttgct ggctgaatac
    17341 caaagtaaat tatctgaata tacattatat aggaacgcgc aatccaatac agtgaaagtg
    17401 attaaggatg ttgatgctgc aattattcaa aacttcagat aatagggagc attcatgaat
    17461 tacatttatc cagtcaatca ggttgatatt atcaaagcca gtgattttca atctcaagag
    17521 atatcaagtc tggaagatgt cgtgtcggct aaatatagtg atattaagat ggatacagat
    17581 attcaagtat cacaaataat ggagatggta agcaatccag aatcattaaa cccagaatct
    17641 ttggccaagt tacagacgac gctctcaaat tattcaatag gagtatcatt agctggcacg
    17701 ttagcaagaa aaacagtttc ggctgttgaa actttattaa agtcttaatt tatatgatta
    17761 ggtataaagg ttttatttta ttcttgttgc tgatgttgat tggatgtgag caacgtgaag
    17821 agttaatttc taatttatct caaagacagg caaatgaaat aatatctgtg ctagaacgcc
    17881 ataatattac tgctagaaaa gttgatggag gtaaacaggg gatctcggta caagtcgaaa
    17941 aggggacatt tgcatcggca gttgatttga tgcgcatgta cgatttgcca aatccggaga
    18001 gagttgatat ctcacaaatg tttcctacag attcattagt gtcttctcca agagctgaaa
    18061 aggcccgttt atatagtgct attgagcaac ggctggaaca gtctttagtt tctattggtg
    18121 gtgttatttc ggcaaaaata catgttagct atgatcttga agaaaaaaat atatcttcaa
    18181 aaccgatgca tatatcagta atcgctatat atgactcacc gaaagagtct gaactattag
    18241 ttagtaatat taagcgattt ttgaaaaaca ccttttctga tgttaagtat gaaaatatat
    18301 ctgtcatatt aactccgaaa gaagaatatg tttatacaaa tgtacaacct gttaaggaaa
    18361 ttaaatcgga atttttaaca aatgaagtaa tatatttatt tctcgggatg gctgtactag
    18421 ttgtcattct tttggtatgg gcattcaaaa cagggtggtt caagagaaac aaaatatgat
    18481 aagaatggat ggaatttata aaaaatatct ttcaataatt tttgatccag cgttctatat
    18541 aaatagaaat cggttgaatt tgccttctga actgttagaa aatggcgtaa tcagaagtga
    18601 gattaataat ctcataatta ataaatatga tctaaattgc gatattgaac ctttaagcgg
    18661 ggtaaccgct atgtttgttg ccaactggaa tttacttcca gctgttgctt attttatcgg
    18721 tagtcaagag tctcggttaa ttaatcatag tgagatggtt attagttatt atgggggcaa
    18781 aatcagtaag caaggggagg ccgctattcg aagtggtttt tggcatctta ttgcgtggaa
    18841 ggaaaatata tctgttggta tatatgaaag aataaactta ttatttaatc caattgcttt
    18901 ggagggaaat tatacgcctg tagaaagaaa tttgtcccga ttaaatgaag gtatgcaata
    18961 tgcaaaaagg cactttaccg gtatccagac atcatgccta tgatggtgtt gttataaaga
    19021 gaattgaaaa ggaattatgt aaaaccataa aagatagaga cactgagagt aagaagaaag
    19081 caatttgtgt tataaaggat gctacgaaaa aagctgaatc actgcgtata gatgctgtat
    19141 gtgatggtta tcaaattggt atacagactg cttttgagca tattattgac tatatttgtg
    19201 aatggaagtt aaagcaaaat gaaaacagaa gaaatattga ggactatata actagtttgc
    19261 tttcagaaaa tttacatgat gagaggatca ttagcacttt attggagcag tggcttagtt
    19321 cgttgcgtaa tacagtaaca gaattaaaag tggttttgcc caagtgtaat ttagcactga
    19381 ggaaaaagtt agaacttgat ctccataaat atcgctcaga tgtcaagatt attttaaaat
    19441 attctgaagg gaataattat atattctgta gtggtaatca agtagtggag tttagtcctc
    19501 aggatgtaat atcaggtgtt aaaatagaac ttgcggaaaa attaacaaaa aatgataaaa
    19561 aatatttcaa agagttagca cataagaaac tgagacaaat tgcagaagac ttgttaaaag
    19621 agaatcctgt taatgattaa tcaaataaat gcaagcaatg ctctgcagca aagattaaat
    19681 agtgaagaat ttgttaattt aaatgagaga ttatcatctt cgcaatcatt tgatgaggat
    19741 ataatctatg aaataatgca atatttctca cagagtgaat taaattccat agataatgat
    19801 gaactgcaca ataagattga acaattattc aattcacgtt ttccttatct tacagctgca
    19861 caaaaaagtt cacttcttaa taaactcata gatgcaaatc aatatgtcga tttgcatgag
    19921 ggattttatg catcattgtc catatataac aatattgatt tctatattaa gactacaacc
    19981 ttcgattcat taattagtgt ctttgaagca gggagagagg cagatgattc gacatggtag
    20041 taataagttg aaaatattta ttttaagtat attgctatta acactgagtg ggtgtgcttt
    20101 aaagtcatca tctaattctg aaaaagaatg gcatattgtt cctgtaagta aggattattt
    20161 ttctattcca aatgatttat tatggtcgtt taatacaacc aataaaagta taaatgttta
    20221 ctctaaatgt attagtggta aggcggttta tagttttaat gcaggtaaat tcatgggcaa
    20281 ctttaatgtt aaggaagtag atgggtgctt catggatgca caaaagatag ctatagataa
    20341 actattttct atgctgaaag acggggttgt tttaaaaggt aataagataa atgataccat
    20401 ccttatagag aaggatgggg aagttaaatt aaaattaatt cgagggatat aattgtattg
    20461 tgagtaaata taaaggtcta aatacaagta atatgtttta catttactct agtggacatg
    20521 aaccagttaa cgttgagctt gtaaaagata aagaacgtaa cataatcgag ctggctccag
    20581 catggaaggg tttttttttg tccgaaatca aaatataaaa ttcagtgata atgtgaacta
    20641 tcattatcgc tttaacatta atagttgtgc aaaatttctt gctttttggg actatttcag
    20701 tggtgctcta gtagagcata gccatgctga aaaatgtatt catttctatc atgagaatga
    20761 tcttcgggat agttgtaata ccgaatcgat gcttgataag ttaatgttaa gattcatctt
    20821 ttcatctgac cagaatgtaa gtaatgcact ggctatgata cgaatgactg agtcatatca
    20881 tttggttctt tatcttctga gaactattga gaaagaaaag gaggtaagaa taaagagtct
    20941 gacagagcat tatggtgtgt cggaggctta ttttagatct ttatgtagaa aagctttagg
    21001 ggcaaaagtc aaagaacaac tgaacacttg gcggttagta aatggtttat tagatgtatt
    21061 tttgcacaat caaacaatca cttctgctgc catgaataat gggtatgcat caacatccca
    21121 tttttcaaat gagataaaaa cacgactagg cttttctgca agagaattat cgaatattac
    21181 ttttttagtg aaaaaaataa atgaaaaaat ttaatattaa atctttgact ctcttgattg
    21241 tattgttacc attgatagtt aatgctaata atattgactc tcatttatta gaacaaaatg
    21301 acatagcgaa atatgttgct caaagtgata cggtaggatc tttttttgac cgtttttctg
    21361 cgttattaaa ttatcctatt gttgttagca aacaagctgc aaaaaagaga atatctgggg
    21421 agtttgattt atctaatcca gaagaaatgc ttgaaaaact gacattactt gtgggactca
    21481 tatggtataa agatggaaat gcattataca tatatgactc tggagaatta ataagcaaag
    21541 ttattttgct ggagaatata tcacttaatt atcttattca gtatctgaag gatgctaacc
    21601 tttatgatca cagatatcct ataagaggaa atattagtga taaaacattc tatatatctg
    21661 gtcctccggc attggttgaa ctggtagcta atacagcaac tttgcttgat aagcaggttt
    21721 cttctattgg gacagataaa gttaattttg gggttataaa attaaaaaat acatttgtta
    21781 gtgacagaac ttataatatg agaggggagg atattgtcat cccaggggtg gctactgttg
    21841 tagaacgttt attaaataat ggtaaggctc tgagtaacag acaagcccaa aatgatccga
    21901 tgccaccctt taatattaca cagaaagtat ctgaggatag taatgacttt tcatttagtt
    21961 cagttaccaa tagttctata ttagaggatg tatcacttat tgcatatcca gaaaccaatt
    22021 ctatattagt caaaggtaat gatcagcaaa tacagattat tcgcgatatt atcactcagt
    22081 tagatgtagc caaacgacat atagaattat cattatggat aatcgatatt gataaatctg
    22141 aattaaataa ccttggtgtt aattggcaag gaacggcttc atttggtgat tcgtttggtg
    22201 cgtcatttaa tatgagctca tcagcatcga taagcacttt ggacggcaat aagtttattg
    22261 cttcagttat ggcattaaat cagaaaaaga aagcaaatgt agtttcaagg ccggtgattt
    22321 tgacacaaga gaatattccg gctatatttg ataataacag gactttttat gtgtcattag
    22381 ttggtgagcg caattcttcg ttggagcatg ttacgtatgg aacattgata aatgttatac
    22441 ctcgcttttc atctcgggga caaattgaga tgtctttaac gatagaggat ggaacaggaa
    22501 atagtcagtc aaattacaat tataataatg aaaatacatc tgtccttcca gaagttggaa
    22561 ggaccaaaat cagtacaata gcgagagttc cacaaggcaa aagtttgttg ataggtggtt
    22621 atacgcatga aacgaactct aacgaaatag tatctattcc ttttctctcc tctattcctg
    22681 taatcggtaa tgtgttcaag tataaaacaa gtaatatcag taatattgta agggtttttc
    22741 tgattcaacc ccgtgagata aaggagtcaa gttattataa tactgcagaa tataaaagcc
    22801 tcatatctga aagagaaata caaaagacta cacaaataat cccatctgaa actactttgc
    22861 tggaagacga aaaatcattg gtttcatact taaattacta actataaagt aggtgatgta
    22921 tgcttgatgt taaaaataca ggagttttta gctctgcatt cattgataag ttgaatgcaa
    22981 tgacaaattc agatgatgga gatgagactg ctgatgcaga gcttgattct ggcttggcta
    23041 atagcaagta tattgactca tctgatgaga tggcttccgc tctttcgtca tttataaaca
    23101 gaagagacct tgagaaactg aaaggaacaa atagtgatag tcaggaacgt attttagatg
    23161 gggaagaaga tgaaattaat cacaagattt ttgatttaaa gagaacgtta aaagataacc
    23221 ttcctctaga tcgggatttt atagacagac taaagagata ttttaaagat ccaagtgatc
    23281 aagtcttagc attaagggaa cttttgaatg aaaaagatct tactgctgaa caagtcgaat
    23341 cattaactaa aattattaat gagataatat caggtagtga aaaaagtgtt aatgctggaa
    23401 taaattcagc tatacaggct aaattatttg gcaacaaaat gaaacttgaa ccacagcttt
    23461 tgcgtgcatg ttatcgtggt tttatcatgg ggaacatatc aacaacagat cagtatatag
    23521 aatggcttgg taattttggt tttaatcaca gacatacaat tgtgaatttt gtagagcagt
    23581 cactgattgt agacatggat tctgagaaac cgagctgtaa tgcttatgag tttggttttg
    23641 tgttatctaa attaattgca attaagatga ttagaacttc agacgtaatt tttatgaaga
    23701 aactggaatc ctcaagcttg ctaaaagatg gcagtttaag tgcagagcag ctattgctaa
    23761 ctttattata tatttttcaa tatccaagtg aaagtgagca aattcttact tctgttatag
    23821 aagtatcacg agccagtcat gaggattctg tagtgtatca aacatatcta tcttctgtta
    23881 atgaaagtcc tcatgatata tttaaaagtg aaagtgaaag agaaattgcg atcaatattc
    23941 tacgagagct tgtcacaagt gcatacaaga aagagctttc tagataacag gagatgaaag
    24001 tgatccagtc ttttcttaag caagtaagta ctaagcctga attaattata ttagtgctta
    24061 tggttatgat cattgctatg ttgatcatac cattacctac ataccttgtt gattttttaa
    24121 tagggcttaa tattgtactt gctattctcg tttttatggg atcattttat attgaaagaa
    24181 tattgagttt ttctacattc ccttcggttt tgttgataac gacattattt cgtctcgctt
    24241 tatcaataag tactagtcga ctaattttag ttgatgcgga tgcgggtaaa attattacta
    24301 catttggcca gtttgtcatt ggtgatagtc tggctgtagg ttttgttatt ttttcaatag
    24361 taactgttgt gcagttcatt gttataacaa aaggatctga aagggttgcg gaggtagctg
    24421 ctcgcttttc tcttgacggt atgccgggga aacaaatgag cattgatgcg gatttaaagg
    24481 ctggaattat tgatgccgca ggagctaaag aaagacgcag tattctggaa cgtgagagtc
    24541 aattgtatgg ttcatttgat ggagcaatga agttcattaa aggtgatgca attgctggca
    24601 tcattattat ttttgtcaat ttaataggag gtatttctgt tggtatgagt caacatggaa
    24661 tgtccctatc tggtgcatta tctacttata ccatactaac tattggtgac ggattggtct
    24721 ctcagattcc tgctctgtta atttctataa gtgcaggatt tatcgttaca agagttaatg
    24781 gtgatagcga taatatggga cgtaacataa tgtcccagat atttgggaat ccttttgttc
    24841 tcattgttac atcagctctt gccttggcga tagggatgtt gccaggtttt ccgttttttg
    24901 tttttttcct gatagcagtt actttgacgg ctttattcta ttataaaaag gtcgtagaaa
    24961 aagaaaaaag tctgtctgag tctgattcta gtggctatac tggtacattt gatattgata
    25021 atagccatga ctcatctttg gcaatgatag aaaatctgga tgctattagt tcagaaaccg
    25081 ttcctttaat tttattattt gccgaaaata agataaatgc aaatgatatg gaaggtctta
    25141 ttgaaaggat aagaagtcag ttctttattg attatggtgt caggcttcca actattttat
    25201 atagaacaag caatgagctt aaggtcgatg atattgtttt gttaataaat gaggtgcgtg
    25261 ccgatagttt taatatatat tttgacaaag tgtgtattac agatgaaaat ggagatatag
    25321 atgctctagg tattcctgtt gtttcaactt catataatga acgtgttatt tcttgggtag
    25381 acgtttcata tacagaaaat ctaactaata ttgatgctaa aattaaaagt gctcaagatg
    25441 aattttatca ccagttgtca caagctttat taaacaacat aaatgagatt tttggtatac
    25501 aagaaacaaa aaatatgtta gatcagtttg aaaatcggta tcctgatcta ttaaaggaag
    25561 tcttccgaca tgtgactata cagagaattt ctgaggtatt acaaagattg cttggagaaa
    25621 atatttctgt tcgcaattta aaacttatta tggagtcttt ggcgctttgg gctcccagag
    25681 aaaaagatgt cataacatta gttgaacatg tccgtgcatc actttctagg tatatttgta
    25741 gtaaaatagc tgtttctggt gagattaaag ttgtgatgct ttccggatat attgaggatg
    25801 caataagaaa agggataagg caaacctctg gtggctcttt cttgaatatg gatatagagg
    25861 tttcggatga ggtaatggaa actttagcac atgctttgag agaattgaga aatgcaaaaa
    25921 aaaatttcgt tcttttggta tcagtagata tacgtaggtt tgttaaaaga cttatagata
    25981 acagatttaa gagtatactc gttatatcgt atgctgagat tgatgaagca tataccatta
    26041 atgtattaaa gactatttag tgaggtttaa atatgagtaa cattaattta gttcaattag
    26101 ttagagatag tcttttcacg attggttgtc ctccatcaat tattacagat ttagacagcc
    26161 attcagcgat taccatttct ttggatagta tgcctgctat taatatagct cttgtcaatg
    26221 agcaggttat gttatgggct aattttgatg caccaagtga tgtaaaactt cagtcatctg
    26281 cttataacat attaaattta atgttaatga actttagtta tagcattaat gagctggtag
    26341 agcttcatcg ttctgatgag tacttacagt taagagttgt tattaaagat gactacgttc
    26401 acgatgggat tgtttttgca gagattctgc atgagtttta tcagaggatg gaaatcttaa
    26461 atgaggtctt ataatcaatg agctatacaa aattgctcac tcaattatct tttcctaata
    26521 gaatctcggg gccaatcttg gaaacaagtc ttagcgatgt ttcgattggt gagatttgta
    26581 acattcaggc tggaattgaa agtaatgaaa ttgttgcaag agctcaggtt gtaggatttc
    26641 atgatgaaaa aacaatatta agcttgattg gaaattctcg tggactttca cggcaaacgt
    26701 tgattaagcc cactgcccag tttcttcata cgcaagtcgg ccgtggatta ttgggagcag
    26761 tagtcaatcc tttaggggag gttactgata agtttgctgt tacggataac agtgaaattc
    26821 tttatcgacc tgtagataat gctcctccgc tatatagtga aagggctgca attgagaagc
    26881 cttttttaac aggtattaag gttattgatt ctttactcac gtgtggtgaa ggacagcgaa
    26941 tggggatttt tgcgtcagct ggttgtggca aaacttttct catgaatatg ctcattgaac
    27001 atagtggtgc tgatatatat gttattgggt taattggtga gcgaggtcga gaggttacag
    27061 aaacggttga ttatttgaaa aactctgaga aaaaaaacag gtgtgtttta gtatatgcaa
    27121 cttcggatta ctcttcggtt gatcgttgta atgctgcata tatagccact gctatagccg
    27181 aattttttag gactgaagga cataaagtag cgctttttat tgattcatta acaaggtatg
    27241 ccagagcatt acgtgatgtg gccttagccg ctggagaatc acctgccaga agaggctatc
    27301 cggtttcggt ttttgatagc ttacccagac ttcttgaaag gccaggaaag ttaaaggcag
    27361 gtggctctat tactgcattt tacactgttc ttttggagga tgatgatttt gctgatccat
    27421 tagctgaaga ggtaagatcc attttagatg gacatatata tttgagcaga aatctagccc
    27481 aaaaaggaca atttcctgca attgattcct taaaaagtat aagcagggta tttacacagg
    27541 ttgttgatga aaaacatcgt attatggccg ctgcatttcg ggagctactt tctgaaatag
    27601 aagagctaag gacaattatt gactttggtg aatacaaacc gggggagaat gcctctcagg
    27661 ataaaatcta caacaaaata tctgttgttg agagttttct gaagcaagat tatcgactcg
    27721 gttttactta tgagcagaca atggagctta ttggtgaaac aattagataa ggttttaaaa
    27781 atcaaagata aatatcaacg gtctgttaaa ttaattgaag cacacatatt aacactaaaa
    27841 aaaaaactct ttgtatcgag atgtggaggc attagataaa aggattatat acttcctgca
    27901 actggagaat gacttagaac ctgtaggcgc tcagagtgtt agtcagcttt tcaatacaag
    27961 acgtaagatc gctatagtaa aaaagcatat tatccaatat cagtctgaac gaattcttct
    28021 aaagggaaga attgaagaaa ttcaaaaaga tattgatgag gcaaatgctt ctaaaagaaa
    28081 actgcttcat aaagagagta aaatatgtaa acggattggc ttgattaaaa gaaataactt
    28141 tgccaaacag ttgatacttg atgagttatc acaagaggat atgaagtatg gcattagata
    28201 atataaacct aaattttagt agtgacaaac agatagaaaa atgtgagaaa ctatcttcaa
    28261 tagataatat tgatagtctc gttttgaaga agaagaggaa ggttgaaatt cctgagtact
    28321 ctttaattgc atctaactat tttactattg ataagcactt tgaacataag catgataaag
    28381 gagaaattta tagtggcatt aaaaatgcgt tcgaacttag aaacgaacga gcgacatatt
    28441 ctgatattcc agaatcaatg gccattaaag aaaatatttt gataccagat caagatataa
    28501 aagcaaggga aaaaataaat atcggcgata tgagggggat cttttcatat aataagagtg
    28561 gaaatgcaga caagaacttc gaaagaagtc atacttcttc tgtaaaccct gataatctgc
    28621 tagaatctga taatagaaat ggtcaaattg gtttaaaaaa tcatagcttg tctattgata
    28681 agaatattgc tgacatcatt tctttactaa atggaagtgt tgctaaatca tttgagctgc
    28741 ctgtaatgaa taaaaatact gcagacataa ccccatccat gtcattgcaa gaaaaatcaa
    28801 tagttgaaaa tgataaaaat gtttttcaaa aaaatagtga aatgacttac cactttaaac
    28861 agtggggggc tggacattct gttagtattt cagtggagtc tggttctttt gttctaaaac
    28921 cgtcagatca atttgtagga aataaacttg acttaatttt gaaacaagat gctgagggta
    28981 attatagatt tgatagcagt caacataata aggggaataa aaataatagt acaggatata
    29041 atgaacagag tgaagaagaa tgctaagaat taaacatttt gacgctaacg aaaaactaca
    29101 gattttatat gcaaagcaac tctgcgagcg tttttcgatt cagacattca aaaataaatt
    29161 tacaggcagt gaaagtttag tcactcttac ttccgtgtgt ggggattggg taattcgtat
    29221 tgatacatta tcttttttga aaaaaaaata cgaggtattt tcaggatttt ctacacaaga
    29281 atctttactg catttatcaa aatgtgtctt tatagagtcg tcatctgtat tttcgattcc
    29341 agaactgtct gataagatta ctttccggat cacgaatgaa atccagtatg caactactgg
    29401 gagtcattta tgctgttttt cctcttcttt aggtattatt tattttgaca agatgccggt
    29461 attacgtaat caagtttctc ttgacttatt gcatcatctt ttagagtttt gcttaggttc
    29521 atctaatgta aggctggcta ctttaaaaag aattcgcact ggtgatataa tcatagttca
    29581 gaaactttat aatttattat tgtgtaatca agttattatt ggggattata ttgtgaatga
    29641 taataatgag gcaaaaatta atctgtcaga aagtaatggt gagtcagaac acacagaagt
    29701 ttctttggca ttattcaatt atgatgatat caatgtaaaa gtggacttta ttcttttaga
    29761 aaaaaatatg acaatcaatg aactaaaaat gtatgtagaa aacgaattat tcaagtttcc
    29821 cgatgacata gttaaacatg taaatattaa agtaaatggt tctttggttg ggcatgggga
    29881 acttgtttct attgaggatg gttatggtat cgagattagt tcttggatgg taaaggagta
    29941 atctcatgct gagtgacatg tccctcatcg ctacactttc tttttttacg ctattaccat
    30001 ttttagttgc tgctggtacc tgttatataa agttttctat tgtttttgta atggtacgaa
    30061 atgcacttgg tttgcagcag gttccatcaa atatgactct caatggtatt gcattgatta
    30121 tggctttgtt cgtgatgaaa cccattattg aagctggtta tgaaaattat ctaaatggcc
    30181 ctcaaaaatt tgatacaata tctgatattg tccgtttttc tgattctggc ttgatggaat
    30241 ataagcaata cttgaaaaag catacagatc ttgagttagc acgttttttc caaaggtctg
    30301 aagaagaaaa tgctgattta aagagtgcag agaataatga ttattcattg ttttccctct
    30361 tgcctgcata tgcgctaagt gaaatcaaag atgcgttcaa aataggtttt tatttatatt
    30421 tgccatttgt tgttgttgat cttgttattt caagcatttt acttgcactt ggtatgatga
    30481 tgatgagtcc aatcacaata tcagtaccaa taaaacttgt attatttgtt gcgcttgatg
    30541 ggtggggtat cctgtctaag gccttgattg aacaatatat caatgttcct gcttagatga
    30601 tggaggtaat gtgtctgata tagtttatat gggtaataag gctctttatt taatccttat
    30661 cttttcctta tggccagtag gtatagctac ggttattgga ttatctattg gtttgttaca
    30721 gacagtgact caactccaag agcagacact tccttttggt ataaagctta taggtgtctc
    30781 aatatctttg ctacttcttt ctggatggta tggtgaggtt ttattgtctt tttgtcatga
    30841 aataatgttt ttaattaaga gtggggtttg atggacattt caagctggtt cgaaagtatt
    30901 catgtgtttt taatactcct gaacggcgtt ttttttagat tggctccatt atttttcttt
    30961 cttccatttt taaataacgg tataatttct ccatctatta gaatacctgt gatttttctt
    31021 gttgcatcag gattaattac ttctggtaag gtagacatag gttcttctgt ttttgaacat
    31081 gtttatttcc ttatgttcaa ggaaataatt gttggcctcc ttctctcttt ttgcttgtct
    31141 cttccctttt ggatatttca tgctgttggt agcattattg acaaccagcg tggggcaacg
    31201 cttagtagtt caattgatcc tgccaatggt gttgatacgt ctgagttggc aaaatttttc
    31261 aatctttttt ctgcagttgt atttctatac agtggtggta tggtctttat tttagaatcc
    31321 atacaattgt cttataatat atgcccgtta ttttctcaat gttctttccg tgtctcaaat
    31381 atcttaacat ttctgacttt attggcaagt caggctgtta ttttagccag tcctgttatg
    31441 atagtattgt tactatcaga agtattactt ggtgtattat cgagatttgc tccgcagatg
    31501 aatgcttttt ccgtatcatt aactattaaa agtttacttg caatatttat tattttcatc
    31561 tgttcttcta ctatttactt ttctaaagtt caatttttcc tcggtgaaca taaatttttc
    31621 acaaatctat ttgttagata aaatattatg gcaaataaaa cagaaaagcc gacacctaaa
    31681 aaactaaagg atgccgcaaa aaaaggacag tcatttaaat ttaaggattt aacgactgtt
    31741 gttattattc tggtagggac atttactata atatcattct tttccttaag tgatgtaatg
    31801 ctcttataca gatatgtaat aattaatgac ttcgaaatta atgagggtaa atactttttt
    31861 gcagtggtta ttgtcttttt taagataatt ggcttcccac tttttttctg tgttctttcg
    31921 gctgtgttgc caacattggt tcaaacaaag tttgttcttg cgactaaagc tatcaagatt
    31981 gatttttcag tattaaaccc tgttaaaggg ttaaaaaaaa tatttagtat aaagacaata
    32041 aaagaatttt tcaaaagcat tctgcttctt attattctag cattaacaac ctatttcttt
    32101 tggattaatg accgaaaaat aattttttct caggtgtttt ctagtgttga tggcttatat
    32161 cttatttggg gggggctgtt taaggatata atattatttt tcttggcatt ttctattttt
    32221 gttattattc ttgactttgt gattgagttc attttataca tgaaagatat gatgatggat
    32281 aaacaggaga taaaaagaga atatatagag caagagggac actttgagac aaagtcgaga
    32341 aggcgtgagt tgcatatcga gattctttca gagcagacta aatctgatat acgtaattca
    32401 aaattagtgg taatgaaccc gactcatatt gcaattggta tttattttaa tccagaaata
    32461 gcgcctgcac cttttatttc tctcattgaa actaaccagt gtgccttggc tgtcagaaaa
    32521 tatgcaaatg aagttggtat accaactgtg cgtgatgtga aattagctag aaaactatat
    32581 aaaacacata caaaatatag ttttgttgat tttgaacact tggatgaagt cctacgtctt
    32641 attgtttggc ttgagcaggt tgaaaacact cattaataag taaaggagat gtgttatatg
    32701 ggagttaatt tctgtaataa aataggtatt gatcagtctg aatttgaaat agaatcttcc
    32761 atcattaact ccattgctaa tgaggtattg aacccaatat ctttccttag caataaggat
    32821 ataataaatg ttttactcag aaagatttca tctgaatgtg acctggttag aaaagacatt
    32881 tatcgttgtg ctctggagtt ggtcgttgaa aaaacacctg atgatttata atcattcata
    32941 ataactcttt tgctgatata aatatttttg ttgaagatat ttatgtagtc acaggatcaa
    33001 ttgtagaaaa ttgttatgtt tttgttagga actgcactaa ataatatatg ataagacagc
    33061 aaaaacgact gaccataatc cttcttttat taggagttga taagagagac tattcatcct
    33121 gtaatgttaa aactctgtta tatagtattc gcgattatgc aaagtctgta aatgatcatg
    33181 agatactaac cgagagtaat cgtttattat ctcattgcat ttcagatagt aacggagctt
    33241 tctttaaaag tagtaaatac gttcccctta agtacttacg taaaaggaga atagcacgaa
    33301 agataccaaa tgattagcac tttgctagta agcttgctgg taaactatat tgaccgttgg
    33361 gtttgttttg agactgtaat ttaaattgtg taattgcctg tttttgatat gtttactcca
    33421 acaacggaga caggtaaatt atggacgaaa agaagcttaa agcccttgcg gctgaactgg
    33481 ctaaaggcct caaaactgag gccgacctca atcagttttc ccgtatgctg acgaaatatc
    33541 ccggctctta aaacgatagt ggctcaggct ttcatttttc agcgttcccc agaagatttc
    33601 catcggagcg ttgtcgtaac agttaccttt acgcgacatt gatgttttca gaccagactg
    33661 ctcctgtatg acccggtaat cgtatgcgca gtactgtgaa cctcgatcgg agtggtggat
    33721 tagcccggca ggtgggtgct ggctcctgag cgccataaac agggctttac ccgtcaactc
    33781 tttcgtcatg cgttctccca tggcgtaacc gacaatttcg cacgtataaa catctttgac
    33841 gccagcgaga tacagccatc cttcccgggt ggcaacatac gttatatctg cgacccagac
    33901 ctgattaggt gctgtgggcg taaagttctg attcagcaga ttcggcgtaa ccggcagatt
    33961 gtggtcagag cttgtggtcg ccctgaactt gcgtttctgc ttacagtgca gcctcagctc
    34021 cttacgaaga cgtgccagtc tgtcacgacc gacgataatt ccgttatctg ccagctccgt
    34081 ctggagccgc cgggttccat atgtttcgcg agtgcggata tgtgccacct taatctccag
    34141 ttttagccgc tcatcacttt gttttctgtc tgagggggca tgctgtaccc ggtcgtaata
    34201 accgctcctg gatacaccaa atacctgaca catcgcttca atgggaaatt gttgtcgcca
    34261 ttgttcga
//
LOCUS       DAETQN010000034        34001 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.034, whole genome shotgun sequence.
ACCESSION   DAETQN010000034 DAETQN010000000
VERSION     DAETQN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.034"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            12..514
                     /locus_tag="K0W68_001651"
                     /pseudo
     CDS             12..514
                     /locus_tag="K0W68_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="K0W68_001652"
     CDS             complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="K0W68_001652"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBI3073238.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="K0W68_001653"
     CDS             complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="K0W68_001653"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBI3073239.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="K0W68_001654"
     CDS             complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="K0W68_001654"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBI3073240.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="K0W68_001655"
     CDS             complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="K0W68_001655"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBI3073241.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="K0W68_001656"
     CDS             complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="K0W68_001656"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBI3073242.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="K0W68_001657"
     CDS             complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="K0W68_001657"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBI3073243.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="K0W68_001658"
     CDS             complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="K0W68_001658"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBI3073244.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="K0W68_001659"
     CDS             complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="K0W68_001659"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBI3073245.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="K0W68_001660"
     CDS             complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="K0W68_001660"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBI3073246.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="K0W68_001661"
     CDS             complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="K0W68_001661"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBI3073247.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="K0W68_001662"
     CDS             complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="K0W68_001662"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBI3073248.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="K0W68_001663"
     CDS             complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="K0W68_001663"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBI3073249.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="K0W68_001664"
     CDS             complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="K0W68_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBI3073250.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="K0W68_001665"
     CDS             complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="K0W68_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBI3073251.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18087..19304
                     /gene="alaA"
                     /locus_tag="K0W68_001666"
     CDS             18087..19304
                     /gene="alaA"
                     /locus_tag="K0W68_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBI3073252.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19388..19987
                     /gene="yfbR"
                     /locus_tag="K0W68_001667"
     CDS             19388..19987
                     /gene="yfbR"
                     /locus_tag="K0W68_001667"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBI3073253.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20046..21878)
                     /locus_tag="K0W68_001668"
     CDS             complement(20046..21878)
                     /locus_tag="K0W68_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBI3073254.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="K0W68_001669"
                     /pseudo
     CDS             complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="K0W68_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(22624..23118)
                     /locus_tag="K0W68_001670"
     CDS             complement(22624..23118)
                     /locus_tag="K0W68_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBI3073255.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23201..23656)
                     /locus_tag="K0W68_001671"
     CDS             complement(23201..23656)
                     /locus_tag="K0W68_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBI3073256.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            23994..25196
                     /gene="ackA"
                     /locus_tag="K0W68_001672"
     CDS             23994..25196
                     /gene="ackA"
                     /locus_tag="K0W68_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBI3073257.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25271..27415
                     /gene="pta"
                     /locus_tag="K0W68_001673"
     CDS             25271..27415
                     /gene="pta"
                     /locus_tag="K0W68_001673"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBI3073258.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27605..29125
                     /gene="yfcC"
                     /locus_tag="K0W68_001674"
     CDS             27605..29125
                     /gene="yfcC"
                     /locus_tag="K0W68_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBI3073259.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="K0W68_001675"
     CDS             complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="K0W68_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBI3073260.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="K0W68_001676"
     CDS             complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="K0W68_001676"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBI3073261.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="K0W68_001677"
     CDS             complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="K0W68_001677"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBI3073262.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31145..31792
                     /gene="yfcG"
                     /locus_tag="K0W68_001678"
     CDS             31145..31792
                     /gene="yfcG"
                     /locus_tag="K0W68_001678"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBI3073263.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31849..32211
                     /gene="folX"
                     /locus_tag="K0W68_001679"
     CDS             31849..32211
                     /gene="folX"
                     /locus_tag="K0W68_001679"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBI3073264.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32232..33125
                     /locus_tag="K0W68_001680"
     CDS             32232..33125
                     /locus_tag="K0W68_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBI3073265.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33173..>34001)
                     /gene="rpnB"
                     /locus_tag="K0W68_001681"
     CDS             complement(33173..>34001)
                     /gene="rpnB"
                     /locus_tag="K0W68_001681"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBI3073266.1"
                     /translation="DTARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLL
                     WSVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFY
                     HGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQK
                     HIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQE
                     KEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8481 a         9280 c         8562 g         7678 t
ORIGIN      
        1 aggaaaaata aatgggaatg attggttatt ttgcagaaat tgactcagaa aagattaacc
       61 aactgcttga gtctactgaa aaacctttga tggataatat acatgacacg ctttcaggat
      121 tacgccgact ggatattgat aaacgctggg attttttaca ctttgggctg acaggcaccc
      181 ctgcttttga tcccgcgaag aatgatccgc tgagtcgtgc cgtgcttggt gaacacagcc
      241 tcgaagatgg catagatggc ttcctgggat tgacctggaa tcaggagtta gccgcgacaa
      301 tcgaccgact ggaatcactg gatcgaagtg agctgcgtaa acagttttcc attaagcgac
      361 tcaatgaaat ggagatttat ccgggagtga ccttttcaga agagttagag gggcaactct
      421 ttgcttcgat catgctgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca
      481 agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg
      541 ccgaacatgt cggcggcttt ctgacttaca aactgatagc ttacatcagc ggcattgcta
      601 accgcacaat gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag
      661 agatcagcac cacgataccg cctgcgctgt actgccagtt tgacggtgca tcgcgacccg
      721 gttgttccgg ggcgtgcagg tacaggctca ccgccacgcg caggtagtag taaaggccgc
      781 ttgccgaacc gacaaccacg gcgcccacca gccaccacaa gtgtgcctgg acaccgaccg
      841 ccagcacgta gaacttacca ataaagccca gcgtcatcgg aataccggcc agagacagca
      901 tcatcaccgt catcaccgcc gcgaggatcg ggcgatgcca gaacagtccg cggtaagagt
      961 acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg
     1021 cgccgaggct gctaaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct
     1081 cgccagtttg cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt
     1141 aaccgagcag acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg
     1201 aggcaaaggc gataatcgcc agcaccacgc gaatcgcttc gctgtcaccc atcggtgcgt
     1261 agaggaacag acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaagaaag
     1321 tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca
     1381 gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct
     1441 cgttgagcat accgtcgcca aggtttttac ccaacgcgac aaacgacagg tcgccagact
     1501 gcgcatacac cagcgccata ccaaacagca ggaaagaaga cgctgcggca gaaaggatgg
     1561 tgtatttgat actggcttcc agtgaacgtt tctggcggaa agcgtaaccg accaggccaa
     1621 acagcggcaa agagatcagt tcgataccga ggaacagaga cgccagatgg ttggcattcg
     1681 ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactcg tccttgttgt
     1741 cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata
     1801 ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag
     1861 cgcctgcctg gccgacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga
     1921 gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg
     1981 tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg
     2041 cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg
     2101 cggagtgcga ggtatccaga atcggctgcg gatagaagcc ctgcagtacc agcagcacca
     2161 ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa
     2221 tctcgctttt cgctttaccg aagtaagcgc gatgcaacat cgccagcgaa taaacagatg
     2281 caaagaccag cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga
     2341 acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg
     2401 caaagaacag cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc
     2461 gcatgtcgcg ggtatggata cgttcataaa gctgaccaca aagaataaac agacccgccg
     2521 ccgacaagcc gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg
     2581 tgtagatagc aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt
     2641 tgatatcggt ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc
     2701 acatagcaat tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca
     2761 gcaaaccgta agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg
     2821 gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg
     2881 cgaaggcgat gaagaagccc agcattaaca ggtattccgc accactggac attggcgtat
     2941 tcagcagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca
     3001 gcgccaggat ggcaatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg
     3061 ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga
     3121 agtacatcgg caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa
     3181 ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat
     3241 atttttcgat ctctttccac gaacatagta ccgccagcac accgagcaga ccggtcagca
     3301 cgaccatcag cagcgacagc ccgtcaatgg cgagatgaat agagataccc aaacgcggga
     3361 tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat
     3421 aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg
     3481 cgatccagcg tggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccaa
     3541 taaagggaat taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc
     3601 ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt
     3661 agtgtcggat gcaccgcgaa ggcggcttat ccgacctacg ttcttatatg cccaggggca
     3721 atcccacaat ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg
     3781 atgctcatgg atgccacata ccagcgcaga tagccgttct cacttaacag cagaccttta
     3841 cctgcaaagc gggaaaggac agccggaatg ttcatcattg agttcagcgg atcgcgtttc
     3901 agcaaccagg caatacccag gaacggcctg acgaacactt tgtcgtacag ccagtcaaag
     3961 ccccaggcgt tgtaccacca ggtactcagc aggcggcccg gcgcactgtt ggcgatggag
     4021 gtcaccagag tacgtttacc cagccacagc caagctgcca gcagaatgcc gacaatcgcg
     4081 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc
     4141 ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc
     4201 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
     4261 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
     4321 accagacctg ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca
     4381 tccttactga agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc
     4441 aggaagcaga gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc
     4501 tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
     4561 aacgcgtgag tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
     4621 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
     4681 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatgcc caccagatgc
     4741 agaatttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
     4801 gttaccatgg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
     4861 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
     4921 agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc
     4981 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg
     5041 aaaacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
     5101 tcggtgtaat agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
     5161 tacatcagca gcaggttgtc ggcaagcacc agaactacca tgctggcgat gaacaggttg
     5221 gtgtaggcga agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg
     5281 tgaataagga aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc
     5341 accaggttaa aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg
     5401 tatgcctgct cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc
     5461 aggcccacag agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag
     5521 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
     5581 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
     5641 gcagcgcaag gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca
     5701 cctgaccgtc ggtctgaccc cagtagcttc cggcgacaac aaaggccagc gcggaggcgt
     5761 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
     5821 ttaagccaag aatgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
     5881 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
     5941 acgctcttca cgaccgacgt ggaaggccac aaccagacct gcgagcagca gcatagaagc
     6001 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
     6061 tggcgtaccg tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag
     6121 catgatggcc gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc
     6181 ctgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
     6241 aatggcaccc gcgtagacga taatttccag cgcaccggcg aagtaagcgc ccagtgagaa
     6301 gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt
     6361 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
     6421 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
     6481 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
     6541 tattccgggt atttgcccgg accggagatc agcagatcct ctttttcgta aaccagatcc
     6601 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
     6661 gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
     6721 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
     6781 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg
     6841 atacgaccac gataacgggg cggcagatag atcggctctt ccgggtacat tcgcgtttcg
     6901 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
     6961 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
     7021 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
     7081 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
     7141 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
     7201 caccgaagaa cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca
     7261 cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct
     7321 gctcggcttc cggctggtca aacgggtgac ggtgacatac cgccacgccc gcgatggcaa
     7381 aggtaataaa accaaagaat tgcgggataa cgttccacac atgcgcctgg ctgttgacga
     7441 tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga
     7501 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcaac gagtatttgt
     7561 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga
     7621 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg
     7681 caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
     7741 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct
     7801 ggagcgaacc gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca
     7861 ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca
     7921 ccgctttgag gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
     7981 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatac cggacatacc
     8041 catcggcaag cccacctgcc ctgccgtcag cccttcagcg atttcaaccg gcagcgtgac
     8101 cgtattgcca tcgtaactaa aggagacgtg tgtacctgcg ttcacaccta acttcgcggc
     8161 atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg
     8221 ctgtgacaat tcatcgctgc caaacaggtg gtaatacggc gcgatacgcc atttcccgtc
     8281 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc
     8341 aaacagacgc acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt
     8401 gttccacgcc tgcggggagt tccagcccgg cgcccaggca aacggcactt gcgaacggtg
     8461 cgcagtcggc tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg
     8521 ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg
     8581 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt
     8641 gatacctgcc agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt
     8701 ccagtccact tcacggctca gcagggtgct gtgcagcgag tgtaaccagc gccagctttc
     8761 cagcatgaca gttttgctgt cgtaataagc aggatcgtaa acctggaaga aacgttgggc
     8821 gcggccttcg ttgttgatca ccgtaccgtc gctttcagca aaactggcag cagaaagtac
     8881 cagatgggcg ttttccataa tcgctgtgcg ttgatgatca accaccatca ccagcggcgc
     8941 tttagccagc gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag
     9001 caccaccacc gcgtcggcgc gtccggtttc cagttcggtc aacgcttctt caagcgaacc
     9061 gccacccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac
     9121 gtcagcgccg cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctat
     9181 gctaccggcg ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac
     9241 gatgacgtcg attttgcttt gcagctcagg ttcgataccg tcaaccgctg gcgcagagtt
     9301 atccagcgca tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
     9361 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
     9421 cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
     9481 tgccgccatt tcgcgcgctt tacctttcac agcctgacgc actgccagcg cgacgcgcgc
     9541 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
     9601 cagagccgga gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc
     9661 ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc
     9721 aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg
     9781 cagaatatct gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
     9841 acgacgctgt actggctgac gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg
     9901 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
     9961 cagttcgccg taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat
    10021 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    10081 aaatacgccg gtcgggcaaa tttcgaccag gttaccagaa aattcgcttt ccagcgtgcc
    10141 gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca gatctgtacc
    10201 gtcagcgtaa tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc
    10261 gtgagagatg aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg
    10321 acggaagctg tgtccggtca tcacagtcat atcctgaaga tggcagttac cgccttcttc
    10381 acataccgga cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa
    10441 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    10501 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    10561 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    10621 cagagacaga caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc
    10681 tacatgaatt gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat
    10741 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    10801 tattgctgaa cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc
    10861 tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga
    10921 atcgacacag ttgctcaagt gtttcgatat cgcccggctg gccttcgcca cgctccagcg
    10981 cacgcagaat tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg
    11041 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt
    11101 caaccgccat cgccagcgcc gtacccagac ggctgcccgc tttaccgata ctttcgaatt
    11161 ccatcggcag atcaaggtgc gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc
    11221 aagctttaaa tttcagacca tcacgcatac caccggcgta atcttcgagg atctcgcgtg
    11281 cggtggtgcc gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca
    11341 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    11401 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc
    11461 atacgccgga ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca
    11521 gggagttgat taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga
    11581 acagttcgaa gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg
    11641 cttcggcaat ggcgcggcgc agatttactg ccgcttcgat atattcgcca cgcaggaaga
    11701 tgtagccacg gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt
    11761 gcggcagttg ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt
    11821 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc
    11881 cagtcgaaaa gcccgctccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga
    11941 tttcgtccgg agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc
    12001 ggtattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    12061 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    12121 cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    12181 ccccaggcag caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt
    12241 gatgttcagc tttttctcga gcgccgcctg aataccctga tacccgttga tatgacagac
    12301 cacgctgtcg caataacgga tcacatggcg accaaccggc tggcggaaga tctgactgta
    12361 gaacgttgcc acaccttcga cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg
    12421 gatcgcacca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    12481 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc
    12541 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    12601 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    12661 cggatcgccg ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga
    12721 taactcatgg tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca
    12781 atcatctgga aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg
    12841 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg
    12901 tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    12961 ctctggcgca gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacct
    13021 ccaaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    13081 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    13141 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc
    13201 gctttctcgt aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga
    13261 tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata
    13321 cggaaacccg tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    13381 aacacgggcg tcattgcgcc gacgtcctga ataaaggtcg agatgtacag caggtggctg
    13441 ttgatacgga acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg
    13501 atcccggcca gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg
    13561 tattcaatac ggtcggtata cggaatgtag ctgtgccagg actggcgctc gcccattttc
    13621 tccgcaccac ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg
    13681 agttgtaaaa cgatacggaa agccccgtgc gccgacgggt ggttcggacc gaggttgagg
    13741 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    13801 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    13861 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    13921 cggcgcaggt tcgggtgacc gtcgaaagta atgccaaaca gatcccaggt ttcacgctca
    13981 taccagttag cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc
    14041 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    14101 gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt
    14161 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt
    14221 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    14281 gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag atgatcacgg
    14341 gtctgccaag cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    14401 ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    14461 atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    14521 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    14581 tcgccaacca cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc
    14641 agtgcctgca tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg
    14701 atgaatttat cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca
    14761 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata
    14821 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    14881 gaagcacgca atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    14941 accatctcaa cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga
    15001 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    15061 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    15121 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    15181 atactgttcg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    15241 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    15301 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    15361 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    15421 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg
    15481 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca
    15541 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    15601 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    15661 cacatcgatt catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat
    15721 acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag
    15781 tttcttcaca ttagtttaca taatatcaac accattagca tttaatgccc tttcacgcca
    15841 gatccttgac gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg
    15901 gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt
    15961 attttcttga tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta
    16021 cgcattttta acatcattgt agcaggtgat ttttttcagg cgattatttg tgcgttcggg
    16081 acgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcact atgcgaggaa
    16141 ggatttttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct cgtttttaca
    16201 ctattgtctc aggaattatc tatcgtccgt cgattactcg atatcccttt caatcaacaa
    16261 tgaatcatcc ccttccggag cagacattgg gctgtattgc cacggattat ggtagctctc
    16321 catcgcctga tagatcacct gggccagctc attattactg gacgggtcat agcacagcaa
    16381 atattcggta tcaggtagcg gcggtaagcc atctactcca ctcagcacac gcaggtacgg
    16441 gctcatcatt tcaatcggcc ttgccgtcac gccaagaccg gctttcactg ccgcacgaac
    16501 ggccggaagc gtcgaggcga cataagccag tcgccatgga atatctgctt tattaagcgt
    16561 cgccagcacc atatcgcgaa acggacttgg atcatccagc aatacaagag ggatcggctc
    16621 gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg tacgcaggtt
    16681 caatgcctta aacgctgaag gacgatgggt ggttaccatc aaatccactt cctgggattc
    16741 cagcatttct gccatatagg cattacgttt aacgcggaca tccagcgcta atttcggata
    16801 aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt catctgaagc
    16861 accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg cctcatcatt
    16921 aaaacgcagt attttcctgg cgtaaccaag aagttgaatg ccatgttcag ttaacagttt
    16981 gttgcgaccg tgacgagcga acagttcttt cccaacgagt tgttccagac gctgcatttg
    17041 ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa aagtgttcag
    17101 atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta tcggacgatt
    17161 tgcacttatc atatattatc acttactggc ggctcatact gagctggtta atgctgtgca
    17221 cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc cggaacccgg
    17281 acgaaagtaa aaatgcatat gagttgcact aaaaaagcga ctcacattgt tccgttataa
    17341 tgcctgaagt agatcacaga atatatcttc agggatcgca tatctattaa gttactcact
    17401 cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc ttgttccact
    17461 ctcgtgcagc atcgctagtc atacgcgaac acgtacaaac agcaatggtg aggcggcatc
    17521 aagagcaggg atccgtcatt tatgcgagca ttttacccca aaaacctttt atttataagg
    17581 gtcattgcga attatctgat gcaaagttat gttaggcaaa gtaatcttct attattaata
    17641 agcacatcaa aacctttttg aatattaaat aataattaat tagcatcatc ctcattcatt
    17701 aattccgctt aacaatagtt tcacaccttg cccccattgc gacacctcgg aaatcatcac
    17761 gctgttaaac tctccgtaat gcggaaatat catcatcgcg tttcattagt gaattcttaa
    17821 atgaggcatt ttcacacaat tatcttacag ataaaaaaac cagacttgca attaagaatc
    17881 agaacaattc actatataac attgcatgca aagcatatac gcctcattat tttgtcatta
    17941 ttaagttatt aacagcacaa tcgagccttc ccctctggca aaatcttatt ctgcagacct
    18001 tcaaaacacc gtcctggggg agtacattgt tctaagctga cttccacggc agggagtggc
    18061 gataacagca aaaaaggtca agattcatgt cccccattga aaaatccagc aaattagaga
    18121 atgtctgtta tgacatccgt ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag
    18181 gtaacaaggt actgaaactg aacatcggca acccagcccc attcggtttt gacgcgccag
    18241 atgaaatcct cgttgacgtg atacgcaacc tgcctaccgc tcaagggtat tgcgattcca
    18301 aagggcttta ctccgcgcgt aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg
    18361 atgttaccgt ggaagatatt tacatcggca atggtgtatc ggagcttatc gttcaggcaa
    18421 tgcaggcatt gctgaacagc ggggacgaaa tgttggttcc tgcaccagat tacccactct
    18481 ggaccgcggc ggtttcgctt tccagcggta aagcggtgca ttatctttgc gatgaatcct
    18541 ctgactggtt cccggacctc gatgatattc gcgctaaaat tacgcctcgt acgcgtggga
    18601 tcgttattat caacccaaat aacccaaccg gcgcggtata ttccaaagag cttttaatgg
    18661 agattgtgga gattgcacgt cagcataatc tcattatctt cgccgatgaa atttatgaca
    18721 aaattctcta cgacgatgct gagcatcact caattgcgcc actggcacct gacctgctga
    18781 ccattacctt taacggactg tcgaaaacgt accgcgttgc aggcttccgt caggggtgga
    18841 tggtgttgaa cgggccgaaa aaacacgcca aaggctacat cgaaggtctg gaaatgctgg
    18901 cttcaatgcg cctgtgtgct aacgttcctg cgcaacacgc cattcagacc gcgctgggtg
    18961 gttatcagag catcagtgaa tttattaccc ctggcggtcg tctttatgag cagcgtaacc
    19021 gcgcgtggga actgatcaac gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc
    19081 tgtatatgtt cccgaaaatc gacgccaaac gctttaacat tcacgacgat cagaaaatgg
    19141 tgctggattt cctgttgcag gaaaaagttc tgttggtgca agggacggca ttcaactggc
    19201 cgtggccgga tcacttccgc attgtcacgc taccgcgtgt cgatgatatc gagctgtctt
    19261 tgagcaagtt cgcgcgtttc ctttctggtt atcatcagct gtaatcttaa tttcactgcc
    19321 ggagattgca tccggcagcg ttatcccgcc acaatgacct gatgatgtca tcatacgtaa
    19381 ggtcactatg aaacagagcc atttttttgc ccatctctcc cgcctgaaac tcattaaccg
    19441 ctggccgcta atgcgcaacg tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc
    19501 gatggtcgcc catgcgctgg cagctatcaa aaatcgaaaa tttggcggta atgtcaacgc
    19561 cgaacgtatc gctttactgg cgatgtacca cgatgcctca gaagtgctca ccggcgatct
    19621 ccctactccg gtgaaatact tcaattcgca aatcgctcag gagtacaagg ctattgaaaa
    19681 aatcgctcag caaaaactgg tcaatatggt tccggaagag ctgcgggata tctttgcgcc
    19741 gttaattgac gagcatgcat atagcgatga agaaaaatcg ctggtgaaac aggcagatgc
    19801 actgtgtgca tatctgaaat gtctggaaga actcgcggcc ggaaataatg aattcttgct
    19861 ggcaaaaacg cgactggaag cgacgcttga agcgcgtcgc agccaggaga tggactactt
    19921 catggaagta tttgttccca gcttccatct ttcgctcgat gagattagcc aggattcacc
    19981 gctgtaagca gccggagtct gcgtcgcatc aggcaataag cgccggatgc gacatcaggc
    20041 tcttgtcaaa acggaaacag catcgggatc atcaccacac aaaccgccat cacgataatg
    20101 gtgaacggta cccccaactt cacaaagtca ctgaagctgt aatttcccgg acctaaaacc
    20161 agtgtgttaa caggtgaaga aaccggtgtc ataaaggcgg cggatgctgc catcgccacg
    20221 accatcgcga atggataagg cgacaccccc atcgttttgg cagcagccag cgcaatcgga
    20281 gccatcaaca ccgccgtcgc ggtattagag ataaatagcc caataaccgc cgacaagaca
    20341 aacaaacacc ccagcatcat atgtggcccg taaccaccgc caatgtccat cagccctttc
    20401 accgccagcg cgacacctcc cgttttctgt aatgccacag caaacggcat catcccaacg
    20461 atcaaaataa tgctcggcca gtgaatggat ttataggcgc tttcagcatc tatacagcgg
    20521 aatttcccca tcagcaggca ggcgatgata gcggcaacag gattaggaat ttcatctgtc
    20581 agcattaacg ccaccattag caccagacag aaaatggcat ggggtgcctg gctgtgcgcg
    20641 ggcgatgctt cactcacctc ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg
    20701 gccagcatac cgatcagttt ccagttaccc acaaccagga tgatatcgcc cagcagcaga
    20761 ggctcatccg ccagcgaacc ttccagcgcc acgccattgc gctttagccc caccacattc
    20821 agtccgtagc gggtacgaaa accaatttcg cgcaccgatt taccaatcag ttctgactca
    20881 ggaattaatg aaatctctgc catgcccaca tcaagggcct ggtcagaaaa atactcgccg
    20941 cgcagtacca tcggctccag caattgctca ctacaaaatt gccggagatc gacatcagcc
    21001 gcagacatat caataagcaa aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc
    21061 acgataacgc gacgaaaacg ccgccagcgt tcaacaccga tgacgttagc gccataacgc
    21121 tcacgtaatt tgagatcatc cagccgttga ccaatcattg gcgatccggg gcgaatagcc
    21181 agacgtcgcg cacgcccggt cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc
    21241 gtccagcctt cgcgctgcgg ggtctgggta tcccctttca gcatgaaacg catcactaac
    21301 atatacaaaa tacccagcac cagcacgacc aggccaatag gtgttacgct aaagaaactg
    21361 aagccgtgat agccttcacg cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc
    21421 accagcgtca tcatgccgct tatcagcccg gcaaaactca acggcatcat cagacgtgac
    21481 ggcgaagttt gcatacgcat ggcaacgctt aacaccacgg gaataaagat agcgacaacg
    21541 ccggttgaac tcataaacgc gccaagcccc gcgacggtca gcatcaacaa aaccaacatt
    21601 ttgatttcac tgttgcccgc aactttgacc agccatgttc ccattacggt ggcaacaccg
    21661 gtacggacca aaccatcgcc aataataaac aaggcggcaa tcaggacaac gttaggatca
    21721 gaaaagccgg aaaatacttc ggggactgtc agcgttccgc ttaatgcaaa cgcgacaata
    21781 acaaacaaag cgaccgcatc catacgcact ctgcccgtcg caaacaagac aatggcaacg
    21841 gccagtaatg aaagaaccca aatcaattca ccgttcacaa cttatccttg ttaattgagg
    21901 gagatgtctt gattctgcca taaaaaagcc ccgacgagac ggggctaaat catgatcagg
    21961 tgtttcactg aataataaca tcgccatttg gctgtttggt cacagtaatt tgctccagac
    22021 tgtggaggac caaatcgacc tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa
    22081 tgacatgaca acccgccgcc aggccagaaa gcacgccagc ggagcatctt ccaccaccac
    22141 acactcctgc ggcgcaagcc ccagcagctg cgcgcctaac agatacgcat caggttctgg
    22201 ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa gcccagctat
    22261 tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc acggaatacc
    22321 tgctttattc aaatgactga gtaaggcgat tgcccccgga agcgggtaat accttcggtt
    22381 tccgtggcct cgatgtgctc cagacgcgta aactcggcgg caatatcagc ctcggatttg
    22441 cccgccataa aatggcgcag agaggtgatc gcctgtttac cgtgaatgaa agccagcacc
    22501 tcttccggcg ctaacccatg acgtctggcc cagttgctcc acgcccgttc taccgcaggc
    22561 agggaatcca ccagcgttcc atcaagatca aacagaaaac ctttgcaccg cacgcgggcc
    22621 tcctcaggca ttgataattt gattaatttc gttggcgctc aaatggtact gacgcgggca
    22681 ggcatgccac acattaagca tgcgctgata tttttcccac attggcgtct gggcgttaaa
    22741 accgtgagtt ccggcgtcaa aatgggtata gcgcccttcc acattaacca taaagcggac
    22801 ataaccgagg taacgtgctt cagtggcggc gtcaaagccg aggaaggtga cacgacgttc
    22861 atcgatggat tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat gatacatctc
    22921 cataatgtcg atgatagtgc ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa
    22981 ttcacgcatc tgtaatccgt aacctcgctc aataattgtt tgcaggcgac ggtaacgttc
    23041 agcatttgcc ggatcgagca tagtcatcat cttgtactga ttagacaaaa taagacgttg
    23101 cgcgttggtc atttccattg ttgactcctg tatcactcta ctacggtgaa aaaaaagaag
    23161 gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag
    23221 ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct
    23281 tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa
    23341 accagttaag gattgcaggg ggtaatggcg ttacagaacg cttgcccagc caccacaatc
    23401 cctgcatggg taaacttaag gcgaacagtg cagtggcaac tgccggccca agctgaccgc
    23461 ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat
    23521 aacgcgtcat cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagtg
    23581 gccacgtctt tgagtaatgc tgtccgcggc gaaacaagct aaaaaaatta acagaacgat
    23641 tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa
    23701 attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta
    23761 tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac
    23821 attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca
    23881 tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac
    23941 gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga
    24001 gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc atcatcgatg
    24061 cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg cctgaagcac
    24121 gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc
    24181 acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg
    24241 cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg
    24301 tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc
    24361 acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag
    24421 acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc
    24481 tctacgccct gccttacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg
    24541 gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag
    24601 aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg
    24661 gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc atgggtaccc
    24721 gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg
    24781 ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga
    24841 ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa
    24901 tggacgttta ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg
    24961 gtcgtctgga cgctgttgta ttcactggtg gtatcggtga aaatgccgcg atggttcgtg
    25021 aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg
    25081 ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta
    25141 tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc
    25201 acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag
    25261 aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac
    25321 cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt
    25381 caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt
    25441 gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg
    25501 tctgctttcc agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc
    25561 taacaccaaa gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca
    25621 gtttgcccag tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt
    25681 tatgtctcag ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa
    25741 cagcttcggc ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc
    25801 accggttgat gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc
    25861 caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc ctctgccggt
    25921 tctcggcgct gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg
    25981 ccacctgaat gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt
    26041 cactttctgc gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct
    26101 ggtgacttcc gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg
    26161 cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa
    26221 actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg
    26281 gcagacctct ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgaacg
    26341 tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct
    26401 gactgccact tctgagcgca gccgtcgtct gtctccgcct gcgttccgtt atcagctgac
    26461 tgaacttgcg cgcaaagcgg gcaaacgtat cgtactgccg gaaggtgacg aaccgcgtac
    26521 cgttaaagca gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa
    26581 tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg cagggattga
    26641 aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg aactgcgtaa
    26701 gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg
    26761 tacgctgatg ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac
    26821 cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt
    26881 atcttccgtg ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat
    26941 caacccggat ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc
    27001 tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg
    27061 tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc
    27121 tgacctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa
    27181 atccaaagcg ccgaactctc cggttgcagg tcgcgctacc gtgtttatct tcccggatct
    27241 gaacaccggt aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg
    27301 gccgatgctg cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga
    27361 cgatatcgtc tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaacctcg
    27421 tcatcatccg cagctttgcg ctgcggatat ctgaaccgga aataatcact atttccggtt
    27481 ttttattctc ttaatctgca ttaatccttt ctgattatct tgcttaactg cgctgcatca
    27541 atgaattgcg ccatttcact ttacatactt accactttgt tttgtgcaag ggaatatttg
    27601 cgctatgtcc gcaatcactg aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt
    27661 ggtgattatc ttttttgttg ctattttaac cagccttgcc acctgggtag ttccggtggg
    27721 gatgtttgac agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt
    27781 cgtagatcca cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg
    27841 cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg
    27901 attaacctca ggatcgaaat acgggacagc tgttggcatc atcatgttta tgctggtgat
    27961 tggcggcgcg tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct
    28021 tattcgccat acccgcggga atgaaattct ctttattcct gcgctgttta ttctgttttc
    28081 acttggcggc gcggtatttg gtatgggaga agaggccgtc gcctttgcca ttatcatcgc
    28141 accgctaatg gtccggctgg gctatgacag tattaccacc gtcctggtga cctatattgc
    28201 cacgcaaatc ggttttgcca gttcgtggat gaacccgttt tgtgtggtcg ttgctcaggg
    28261 gattgccggc gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc
    28321 cactatgatt ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct
    28381 tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca
    28441 acgtccgttt acctttggtg actggctggt attgattgtc ctgaccgccg taatggtctg
    28501 ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt
    28561 caccatgggt ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt
    28621 taataccatg gcttcatcct ttaccgaagg gacgcgaatg atgatcgccc ctgccctgct
    28681 ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag
    28741 cgtgttaaat accatcctca acagcattgc caatgccatt agcggtctgg acaacgcggt
    28801 cgcggcctgg tttatgttgc tcttccaggc ggtatttaat ttcttcgtga cgtccggttc
    28861 tggtcaggcg gcgttaacca tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa
    28921 ccgtcaggtt accgtgctgg cattccagtt tggtgatggc ttcagtcaca tcatttaccc
    28981 aacctcagct tcgttaatgg caactctcgg tgtgtgcagg gtggacttcc gtaactggct
    29041 gaaggtgggc gcgaccctac ttggactgct gtttattatg tccagcgtcg tagtgatcgg
    29101 cgctcagttg atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca
    29161 ttctgccgtt tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag
    29221 cgctttcagc gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag
    29281 ccagcaaact tcactgactt catcttcctg tagggcgaag ggaccgtgag agacgcagct
    29341 gaacaacgcg ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc
    29401 aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag
    29461 ttgctcatcg gcctgaacca caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc
    29521 ttttgtctcg gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta
    29581 agttgcacga tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac
    29641 ttcgttctct tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat
    29701 tatcaggaaa ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta
    29761 cggattaatc gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac
    29821 gtcattatcc agcatgccat aactcgccgg attaccgcct ttcggaatac tcaccgagcc
    29881 ggggttgaag tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta
    29941 caccagcaca tcgttctggt ttaaagcagg tagattttcc gggccaaaaa gatgaccatg
    30001 cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa
    30061 atgcagcagc atttgatcca cttcgctgtc gcagttgccg cgcacagcga taaccttatg
    30121 tgccacttca ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt
    30181 acgcgggccg tgattcaaca cgtcgccaag gatcaccagc cactgggcac cgctttgggc
    30241 aaacaactcc agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat
    30301 cagtttcatc actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt
    30361 gctatcagcc agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc
    30421 actggaatgt cgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc
    30481 ggttaatcat tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct
    30541 gacccagtgc taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct
    30601 cggccgttag tggcgctttt ttcgcccccg caaagacaac atccgtcgga cgctcttcgc
    30661 ggatgggcat cagatcgctg cgcagccagg cctgaatccg tcgtgcacgc gcacgatttt
    30721 ctaaatcaag cggataaata cgttcccagg tcggtggcgc aaatcgatct tccagatact
    30781 ccgcaatggc agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc
    30841 gtgtctgacc gtaaccttgc cacgtcggct tcaaatgttc accgctgtcg aggtcgatgg
    30901 tcttgatatg aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag
    30961 gggagaaaaa gtgggcatct gaccaaagcg tgatagcggg tttactcatt atgtcctcgg
    31021 caataaaggg ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata
    31081 ctcacgtaag tcgccgcgct ttctatactt gtggtgttca gatgattaaa caaccggagc
    31141 tgcaatgatc gatctctatt tcgccccgac acccaatggt cacaaaatta cgctgtttct
    31201 cgaagaagca gagcttgatt atcgcttgat taaggtagac ctggggaaag gcggtcagtt
    31261 tcgtccggaa tttttgcgca tttcgcccaa caacaaaatt ccggcaattg ttgatcattc
    31321 tcctgccgat ggcggcgaac cgctaagcct ctttgagtct ggtgccattt tgttgtatct
    31381 gactgagaaa acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca
    31441 gtggttattc tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa
    31501 tcacgcagcc ccccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg
    31561 tctttaccat gtactaaaca agcggctgga aaactcgccc tggctgggag gcgagaacta
    31621 cagcattgcg gatattgcct gctggccgtg ggttaatgcc tggactcgcc agcgaattga
    31681 cctcgcaatg tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac
    31741 cgggcaggca ctgctaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca
    31801 ggttctcgtg tattatttca tcctaagtaa aacaacggag aacctgcaat ggcacaacct
    31861 gccgctatta ttcgtataaa gaaccttcgt ttacgtacgt ttatcggaat taaggaagaa
    31921 gaaattaaca accgtcagga tattgttatc aatgtgacga tccactaccc cgccgataaa
    31981 gcgcgcacta gcgaagatat caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt
    32041 attcagcatg tagagaataa ccgtttctct ttgttggaaa aattaactca ggatgtgctc
    32101 gatatcgcac gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactgcac
    32161 gcgctgcgct acgccgattc ggtatccatg accttaagct ggcagcgtta accgccatat
    32221 cgggaggctg catgaacata gtgatcaccg gagggacggg attaattggt cgccatttga
    32281 ttccgcgttt gctggagctg ggccatcaaa tcacggtagt gacgcgtaac ccacagaaag
    32341 ccagttccgt tctcggccct cgggtgacgc tatggcaagg gctagccgat caaagcaacc
    32401 tcaacggcgt tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga
    32461 ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg
    32521 atttgattaa tgccagcgac acgccgccgt cggtattcat ttccggctcg gcaacgggct
    32581 attatggcga cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta
    32641 ctcataaact ctgcgcccgc tgggaagaga ttgcctgccg ggcgcaaagt gacaaaacgc
    32701 gagtgtgcct gctgcgaacc ggcgtggtac tggcaccgga cggcggtatt ctcggtaaaa
    32761 tgctgccacc gtttcgtctt ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg
    32821 cctggattca tattgatgat atggtcaacg gcattctctg gctgctggat aacgacctgc
    32881 gcgggccatt taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc
    32941 tcggtcaagc gctgcatcgc ccggccattt tgcgcgtccc cgcaaccgcc attcggctgt
    33001 taatgggcga atcttcaata ctggtattag gtggacaacg cgcgctgcct aaacggctgg
    33061 aagaagcggg ttttgcgttt cgctggtacg atttagaaga ggcgctggcg gatgtcgttc
    33121 gctgatgtgg tttacagcaa acatccgcca gttaactccc ggtgttacag gattagtggc
    33181 tttgcgcgat aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctctgtcta
    33241 aacctctatc cagcatctcc tgagcaatac gcagggcttc ttcgtgtttg ccctgcattg
    33301 cgccttcttc acgtaatctg tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc
    33361 gttctgttat ctcaccaata aatgcacgga aacgctgggc atcccctgtt tgtaatacgt
    33421 aattaaacag ggcttttagc tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa
    33481 tttggtcgac taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca
    33541 gcgccatttt gcggtgttgc ataatctcgt catccggcac cacggtaata tccaccaacg
    33601 gaaacgctga tgaatatatt ttgcgggcta tagcaggctc ggcaaattca tcaagccagc
    33661 agagtgaata aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg
    33721 gaagctcttt atagcccgca tcaagatggt tttgcattgc cgcaatggaa taacgcatca
    33781 tgcgaaaagc cattaattct tccagcttac tttggtgctc tatcactaca taaatataac
    33841 ccactccctc ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtctt
    33901 catcaataaa actgtttgat tctagtttaa gcgtcgttaa atcacacagt ttgcgcagcg
    33961 gcgcgggaag atgaatatca ataaaatccc gcgcggtgtc t
//
LOCUS       DAETQN010000035        33995 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.035, whole genome shotgun sequence.
ACCESSION   DAETQN010000035 DAETQN010000000
VERSION     DAETQN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.035"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..2601)
                     /gene="acrF"
                     /locus_tag="K0W68_001682"
                     /pseudo
     CDS             complement(1..2601)
                     /gene="acrF"
                     /locus_tag="K0W68_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="K0W68_001683"
     CDS             complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="K0W68_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HBI3073267.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4169..4831
                     /gene="acrS"
                     /locus_tag="K0W68_001684"
     CDS             4169..4831
                     /gene="acrS"
                     /locus_tag="K0W68_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HBI3073268.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(4834..5013)
                     /locus_tag="K0W68_001685"
     CDS             complement(4834..5013)
                     /locus_tag="K0W68_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBI3073269.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(5097..5981)
                     /gene="yhdJ"
                     /locus_tag="K0W68_001686"
     CDS             complement(5097..5981)
                     /gene="yhdJ"
                     /locus_tag="K0W68_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBI3073270.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6067..6363)
                     /gene="fis"
                     /locus_tag="K0W68_001687"
     CDS             complement(6067..6363)
                     /gene="fis"
                     /locus_tag="K0W68_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBI3073271.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(6389..7354)
                     /gene="dusB"
                     /locus_tag="K0W68_001688"
     CDS             complement(6389..7354)
                     /gene="dusB"
                     /locus_tag="K0W68_001688"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBI3073272.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(7683..8564)
                     /gene="prmA"
                     /locus_tag="K0W68_001689"
     CDS             complement(7683..8564)
                     /gene="prmA"
                     /locus_tag="K0W68_001689"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBI3073273.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(8576..10027)
                     /gene="panF"
                     /locus_tag="K0W68_001690"
     CDS             complement(8576..10027)
                     /gene="panF"
                     /locus_tag="K0W68_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBI3073274.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10017..10259)
                     /locus_tag="K0W68_001691"
     CDS             complement(10017..10259)
                     /locus_tag="K0W68_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBI3073275.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(10368..11717)
                     /gene="accC"
                     /locus_tag="K0W68_001692"
     CDS             complement(10368..11717)
                     /gene="accC"
                     /locus_tag="K0W68_001692"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBI3073276.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(11728..12198)
                     /gene="accB"
                     /locus_tag="K0W68_001693"
                     /gene_synonym="fabE"
     CDS             complement(11728..12198)
                     /gene="accB"
                     /locus_tag="K0W68_001693"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBI3073277.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13176..14150)
                     /locus_tag="K0W68_001694"
     CDS             complement(13176..14150)
                     /locus_tag="K0W68_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBI3073278.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            14302..16242
                     /gene="csrD"
                     /locus_tag="K0W68_001695"
     CDS             14302..16242
                     /gene="csrD"
                     /locus_tag="K0W68_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBI3073279.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAAQLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            16547..17590
                     /gene="mreB"
                     /locus_tag="K0W68_001696"
     CDS             16547..17590
                     /gene="mreB"
                     /locus_tag="K0W68_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBI3073280.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            17656..18759
                     /gene="mreC"
                     /locus_tag="K0W68_001697"
     CDS             17656..18759
                     /gene="mreC"
                     /locus_tag="K0W68_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBI3073281.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            18759..19247
                     /gene="mreD"
                     /locus_tag="K0W68_001698"
     CDS             18759..19247
                     /gene="mreD"
                     /locus_tag="K0W68_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBI3073282.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19256..19849
                     /gene="yhdE"
                     /locus_tag="K0W68_001699"
     CDS             19256..19849
                     /gene="yhdE"
                     /locus_tag="K0W68_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBI3073283.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            19839..21308
                     /gene="rng"
                     /locus_tag="K0W68_001700"
     CDS             19839..21308
                     /gene="rng"
                     /locus_tag="K0W68_001700"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBI3073284.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            21376..25176
                     /gene="yhdP"
                     /locus_tag="K0W68_001701"
     CDS             21376..25176
                     /gene="yhdP"
                     /locus_tag="K0W68_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBI3073285.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            25332..26777
                     /gene="tldD"
                     /locus_tag="K0W68_001702"
     CDS             25332..26777
                     /gene="tldD"
                     /locus_tag="K0W68_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBI3073286.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(26905..27770)
                     /locus_tag="K0W68_001703"
                     /pseudo
     CDS             complement(26905..27770)
                     /locus_tag="K0W68_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            27953..28156
                     /locus_tag="K0W68_001704"
     CDS             27953..28156
                     /locus_tag="K0W68_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBI3073287.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            28164..29096
                     /gene="aaeA"
                     /locus_tag="K0W68_001705"
     CDS             28164..29096
                     /gene="aaeA"
                     /locus_tag="K0W68_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBI3073288.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            29102..31069
                     /gene="aaeB"
                     /locus_tag="K0W68_001706"
     CDS             29102..31069
                     /gene="aaeB"
                     /locus_tag="K0W68_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBI3073289.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            31161..31433
                     /locus_tag="K0W68_001707"
     CDS             31161..31433
                     /locus_tag="K0W68_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBI3073290.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(31489..31752)
                     /gene="yhcN"
                     /locus_tag="K0W68_001708"
     CDS             complement(31489..31752)
                     /gene="yhcN"
                     /locus_tag="K0W68_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBI3073291.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(32117..32587)
                     /gene="argR"
                     /locus_tag="K0W68_001709"
     CDS             complement(32117..32587)
                     /gene="argR"
                     /locus_tag="K0W68_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBI3073292.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            33022..33960
                     /gene="mdh"
                     /locus_tag="K0W68_001710"
     CDS             33022..33960
                     /gene="mdh"
                     /locus_tag="K0W68_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBI3073293.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         8255 a         8454 c         8900 g         8386 t
ORIGIN      
        1 tcctgatacg acatacctgt ccagtcataa ccaatgcccg caggtaattt tgacgcaagg
       61 ttttccatca atgccatggc atcgccggaa ctggttcctg gcgcggcttc cccctgaatc
      121 tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac ccaatgtgaa
      181 gtggtaaagg cagagaatgg caccatttcg ccgttggcgc tgcggacata aagtttatcg
      241 acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt tttcacgcga
      301 ccacggtcga tgaagtcgtt aacgtaagtc ccacccagcg ccgttgaaat ggtctgattg
      361 atgtcagaaa gtgaaacacc taatgcctgc gccttttcct ggtcaacttc cagtttaaac
      421 tgcgcggtgt cttccagacc attaggacgc acgctgacta agctggcagg atgttgcgcc
      481 gccatgccaa gcaactggtt acgggcctgg gtcagggcat cgtgacccag cccagcctga
      541 tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc tggcatattg
      601 aatggaatga caaaaccgtc gcggatcttg cccaattcca ttttggcacg atggattacc
      661 gcttccgcac tgttttcgtc accactacgc tcttcccacg gtttcagact gacgaaagcc
      721 ataccggcgt tttgtgcctg gccgctgaag ctaaagccgt taaccgtaaa gacactttct
      781 acgttcgctt tctcgttatt cagatagtaa tccgtcactt gatccaacac tttttgcgtc
      841 cgctcttgcg tcgcgccagc gggtaactga atcatggtca gaaagacacc ctgatcctct
      901 tcaggtaaga aggaagaagg aagacggaag acgtaaaaac aacaccacca ttcctgcaac
      961 aatcagcgca tagatcagta aatatcgtcc tgtggaaccg aggattttgc cgacgctgtt
     1021 ggtgtagtgg ttaacgctat gatcgaaggt ggtattaaac cagccgaaga aaccgccctt
     1081 attttcgtga tgctcagcag agacgggttt aagcagcgtt gcacataacg caggggtaag
     1141 aatcaatgcc accagaacag aaagcgccat tgccgaaacg atggtgatag agaactggcg
     1201 ataaattgcc ccagtagaac cgccgaagaa tgccatcgga ataaataccg ctgacagcac
     1261 catcgcgata cccaccagtg cgccctgaat ttgcgacatc gatttttccg tcgcttcttt
     1321 tggcgggagc ttatcctcca tcatcacgcg ctcgacgttc tccaccacca ctatcgcatc
     1381 atcgacgagc agcccgatgg caagcaccat cccgaacatc gttagtgtgt tgatggagta
     1441 accaaaagcg gcgaggatgg caaacgtccc taacaacacc acgggtaccg caatggtggg
     1501 gatcagcgtt gctcgcatat tctgcaagaa cagatacatc accaggaaca ccagcataat
     1561 ggcttcgaac agcgttttta ccacttcgtg aatagaaagc tggacgaatg gcgtggtgtc
     1621 ataagggtag agaaccttca ttccctgcgg gaagaatggc tgtaattccg ccagttttgc
     1681 cttaatggct ttcgcggtat tgagagcgtt cgcgccggtt gctagcttaa tccccaggcc
     1741 cgccgccggt tttccgttga tacgagcgat aacgttatag ttttcaccgc caagttcaac
     1801 tcgtgcgaca tctttcaggc gtaccaccga gccgtcactg tttacgcgca gggttacttt
     1861 gccgaattct tccggatttt ttaaccgcgt ctgagcaata atcgaggcgt tcaactgttg
     1921 ccctggtaac gctggcgttc cgcccaactg cccggcagcg atctgatcgt tctgtacctt
     1981 caactggtta atcacatcaa ccggtgtcag tttatatttg tttagcagat cggcgtccag
     2041 ccagatacgc atcgcatact gtgcgccgaa aagctgtacg tcaccgacgc cattcagacg
     2101 gctaagcgta tctttaacgt tagaggccac atagtccgag atatcgtcct gtgtggtgtc
     2161 tgggttatca gagacaaagc ccgccaccat caaatagctg ctactggact tttcaacact
     2221 gatcccctgc tgctgaacct cctgcggcag caacggcgtg gcgagctgga gtttgttctg
     2281 cacctgcact tgcgcgatat caggatcggt cccggactgg aaggtaaggg taattgtcac
     2341 gctaccggcg gaatcgctgg tggaggacat atacatcagg ttatcgatac cattcatatt
     2401 ctgttcgata acctgcgtca ccgtatcctg cacggtctgc gcatcagcac ctggatagtt
     2461 tgcctaaaca gaaaccgcag gcggtgcaat ggttggatac tgagcgacgg gcaattgtag
     2521 gatcgccagt gcgcccgcca tcatcagaat aatggccagc acccatgcaa atatcggtcg
     2581 tcgaataaaa aagtttgcca tgtcagatta ccttacttcg atgcagtatc tgcgggggta
     2641 tcggtagtgg ctttaacctg ctctcccgga cgcgcttttt gcaggccgct gacaatgact
     2701 tgatcgccag atttcagacc ttcgctaatc aaccatttat cgccaatcgc ctgactggca
     2761 acgacagggc gcgcttccac ctgactttta tcgttaacaa tcagcacggt tgcatcacca
     2821 cgcggtgtgc ggctaacgcc ttgttgcggg ataagaatgg cgtcaggttg gacgccttca
     2881 tcaatccgtg cacgcacaaa catacccggc aaaagcgtat gttgcgggtt agggaagaca
     2941 gcacgtaggg ttatggagcc ggtgcattca tcaacggtca catcagagaa ttgcagcgta
     3001 cctttcaggg gataggtttg accgttttcc atgaccaact ctacgttgct ggtggcgttt
     3061 tccttatgca aatttccttg ctctacggat tgcttcagcc tcataaaatc gttgctggat
     3121 tgggtcacat caacgtagat aggatcgagc tgctggacag tcgccagttc agtcgtttgc
     3181 ccattagtga caagagcgcc ttcggtcacg gtcgatttgc cgatacgtcc gctaattggc
     3241 gcagtcactt tggtataagc gagattgatg cgagcgcttt cgactgtggc ttttgcggca
     3301 atcacggcgg catcggcctg gcgagcatca gcaatggcct ggtcgtactc ctgctggctg
     3361 atgtatttcg tacccacgag cggaacgtaa cgttttaccg tcaaatgcgc gatggcggcg
     3421 gcggcttcac ttttcgccag ttcgcctttc gcgctgtcat aatttgcctg ataggtcgcg
     3481 ggatcgatct ggtacaggga ctggcctgct tgtacatcgc tgccttcagt gaaattacga
     3541 ttcagtacga tcccgctaac ctgtgggcga acttcggcta tacggtaagc attggtgcgg
     3601 cctggtaatt cagtcttaac ttctaacggg gccgttttca caatatgaac ggtaacctgc
     3661 ggttcaccga cgtgagcttt ctcttctccc ttatcgttac aaccgacgat taaagccgcg
     3721 gagatcagaa taaaggaggg caggaggaaa aacctggcat gtttcgtcat tactattcct
     3781 caaaaaaaca aaagcgcgtt atttacccag aaaggcaacg cgttaactcg cagaaagaaa
     3841 aatacagttt gctatcctac aaattatcat tcgtcgatgt aaggaatagt tatgaatact
     3901 ggcgtctcaa ggcacataaa cacaaaaaaa gattaatatt ctactgtttt attttgacgc
     3961 gggttgaaag aggcagaatt aaaacctcgt aaattgaaat atatattcat gtggcgaatg
     4021 tattttaggt aaataatata tattatttat ttgacgattt atagcaactc aatgttaccc
     4081 tctcgtataa atacacatta gatgatagat taaccttcgc tattttctca ccctgtgtcg
     4141 aatatattta tttcctgaat aattaatcat ggcaaaaaga accaaagccg aagctctgaa
     4201 gacccggcaa gaactgattg aaactgccat cgcccagttt gcgcaacatg gcgtaagcaa
     4261 gacgacgttc aacgacattg ccgatgccgc taacgttacg cgtggcgcta tctactggca
     4321 cttcgagaac aagacccaac tgtttaatga gatgtggttg caacagcctt cattgcggga
     4381 gttaatccag gaacacttga cggctggatt agagcatgac ccgtttcaac aattgcgtga
     4441 aaaattgatt gtcggcttgc aatatattgc caaaattccc cgccagcagg cgttgctgaa
     4501 aatcttatat cacaaatgtg aatttaatga tgggatgctg gccgagggag tgatacgcga
     4561 aaagatgggc tttaatccgc agactctccg cgaagtattg caggcgtgtc agcaacaagg
     4621 ttgtgtagca aataacctcg atttagatgt tgtgatgatt attattgatg gtgccttcag
     4681 cggaattgtt caaaactggt taatgaatat ggcgggttat gatctttata aacaagcacc
     4741 cgcgctggtc gataacgtat taagaatgtt catgccagat gaaaacataa cgaaattaat
     4801 tcatcaaaca aatgaattaa gtgtcatgta aattcacatt ctgtcctgaa aattcagttc
     4861 gtcacagtta accagtttta gtggataaac tgagttcata ttacggcatt tgtctgtttc
     4921 tgttgtcagc agttcaatcc actgcatcag taaagtatca aacagaaaaa cgaagacagc
     4981 gaaaacgacg agccaccaat acttgcgaat cattacctga gcctgtgcat gtttaaccaa
     5041 gtctggcagg aaatatacac gaaatgaata atctgaaaac caggcttaca cttcctttac
     5101 tttgcaatga gatcggggtc aacttcgctt aaccggcttc gttttgacag gttgccggtc
     5161 tttctttttt tcactttcgc cagttcttcc gcggagtaat gcgacgcgac atccagccgt
     5221 cgaagcccca ttttgatgta ctcgctgttg atctcaatac caatgaattt tcgtccgctg
     5281 gcgatggcta cggcaccggt agtaaagcta ccggcaaacg ggtcgagaac gatatcgcct
     5341 gggttggaag aggcgagaat aatgcgtttc agtaaggctt ccggtttttg cgtcgggtgg
     5401 ttctcatatt catccatcaa ataacgcacg cgcggaaaat cccagacgtt acccggtact
     5461 ttttgatgat tgtatggctg tggaggattt ttgcgataat cgatcaacgc gcgctgcgat
     5521 ccggttttgg cttctaccag aatagcatca ccgttgaatg tgtagttctt tgcgtctttc
     5581 accatcatca ggatgggttc gtacatggag ccgtagtgtt ttttcgcctg tactccagaa
     5641 ctgtcatatg accagacgat gcgacttttg atggtaaaaa gcttgcggca ctggagatcg
     5701 ataaagggca tgttttccgt actgttcata atgtacatgc tgccctgctt tttcagaacg
     5761 cggtggcact ctacaatcac ttcaaacagc cagtcgataa acagatcttc tttccaggct
     5821 tcgatcagac catcaaaatt tttaccgatg ttatacggtg ggtcggcaaa gatcagatcg
     5881 acgctttcag taggtagctt tttaagttcg gcaagggcat caccgtgaat aatggtctta
     5941 gcttcattac caaaccgggt cggttcacat cctgttctca tggtcactcc ctttgtgaca
     6001 cctataaaaa aggcgcttcc ccatgccgag tagcgccttt ttaatcaagc atttagctaa
     6061 cctgaattag ttcatgccgt attttttcaa ttttttacgc agcgtaccac ggttgatgcc
     6121 catcatcagc gcagcacggg tctggttacc acgggtgtat tgcatcacca tgtccaacag
     6181 gggctgttct acttcagcca gtaccagctc atagaggtca ttcacatcct gaccattcag
     6241 ttgagcaaaa tagttcttca gtgcctgttt aaccgagtca cgcaggggtt tttgggttac
     6301 ctgatcctga gagttaacgg tagaaacggt cagtacgtca gaatttacgc gttgttcgaa
     6361 catagttctg tcagctcttt atttctgttt acgcaaaatt ttcgaagtat gcctccaacg
     6421 cctccagctg ttcgctggca tcctcaatgg cgttgaatgt gcgccgaaac tggtcatttg
     6481 gagcgtgttc ctggagatac caggaaacgt gtttacgtgc aattcggtac ccttttgccg
     6541 gaccataaaa gtcatgcagt tcccgaacgt gcgcgcaaag caagcgctta acctctgcca
     6601 aaggcagcgg gggcagcaac tccccagtgt ccagataatg ctggatttcc cgaaagatcc
     6661 agggtcttcc ctgagctgcg cggcctatca tcagggcatc cgcccctgta tagtcgagca
     6721 cagctctggc tttaagcggg tcagtaatgt cgccattcgc gataaccgga atggaaactt
     6781 tctgcttaac tgcccgaata ctgtcgtact cagcttctcc attgaacaaa caggcgcgtg
     6841 tacggccatg aatggtcaga gcctgaatgc cacagtcttc agccagttgg gcaatctctt
     6901 cgcagttacg gtgttccggt gcccagccgg tgcgaatctt cagggtaaca ggaacgtcca
     6961 ctgcattgac gacctcggta aggatcgatt taacgacatc cgggtactgc aagagggctg
     7021 aacctgcgag cttgcgattc acttttttag ccgggcaacc catattgata tcaataatct
     7081 gggcaccgct ttccacgtta atacgtgctg catctgccat ttctttcgga tcgctaccag
     7141 caatttgcac ggtgcgaata ccgggttcat caatgtgcac catccgtaaa cgagatttgt
     7201 cgctttccca aacctgtggg ttagaagaca tcatctcgga tactgtcaat ccggctccca
     7261 tctcgtagca caacgtccga aaaggtctgt ctgtaatgcc agccatgggc gctgcgatca
     7321 ggcgatttct gagctgatat tgtccgatgc gcatgagtta agaaatgacc atactgtgac
     7381 tgcaaggcgg cgtatattac gcattttttg cacgagatga aaggccaaac tttgaccaat
     7441 cctctgagat ggatcaaaga attgcattta aaatgagcgt ggtgagataa ttactcataa
     7501 aaatcatcat attagaaaat agtgactaaa atttacactc aaagaaattt gagtaagttc
     7561 tcaatttttc tttatgaatg aaaattttgg cacgcaaatt tgcgtaaata accggcaaat
     7621 ttacgtgcct tttgtgagct tgctcgcact tcgccccgcg tcaccctacg gcgatgcgaa
     7681 ggttaattct tacgaccggt aatgcggcac cactcttctt tttccacgac cgggtccagt
     7741 gcgaagctgt cgacataagc ttcacaaacg ctctctgcct ggcttgccag aataccggaa
     7801 aggcccagca aaccgcctga aaccggcagg acgctgatta acggtgccag ttcacgtaat
     7861 gggcctgcaa ggatgttagc gaccaccacg tcggctttca tttcttctgg ctgatctttc
     7921 ggtaagtaga gttccagacg gtcagaaacg ccattacgtt cggcgttgtc gcggctagcc
     7981 tgaatcgcct gcggatcgat atcaatacca atggcttttg ctgcacccag tttcagcgcc
     8041 gcgatagcca gaatgccgga accacagcca aagtcgatga ctattttacc ggttaaatcg
     8101 aggctgtcga gccactgcag gcacagagag gtagttggat gggtacccgt accaaacgcc
     8161 agccctggat ctaacatcac gttgacggcg ttttcgtccg gcacatcacg ccagctaggg
     8221 cagatccaca gtcgttcacc aaagcgcatc gggtggaaat tatccatcca ttcgcgctcc
     8281 cagtctttat cttccagttg ttcgatttta tgcgcgaagc ctgcgccgag cagcggatgg
     8341 ttttccagaa tcgccaccac gtcgttcata tcggtttcag cgtcgaacag accaatcaca
     8401 tcggtgtcgc cccacaggcg cgtttcgccc ggcagcggct caaatactgg cgtatcgtgg
     8461 gtatcctgaa aagtgataga aacggcaccc gcttccatca gcgcatcgct aagatcttca
     8521 gcgttcgcgc cggtggtgtt cagtttcagt tggatccaag gcatggcaaa actctttatt
     8581 tatcagtagt caaaacggta gcttgcggga cggatgtacc gaaacggttt ccgaccagga
     8641 aagccagcaa acttagtagt aacgagggca cgatagggtg gaagcccagg tactgaatat
     8701 tcagcgtcgc gagtacggca tacagcacgc cgccaacgat catcgcactt agcgcgcctt
     8761 tggcgttggc gcgttcccag taaagaccca gcaccagcgg ccacaggaaa acggcttcca
     8821 gcccaccgaa ggccagcaaa ttcagccaga tgatcatttc tggcggcttc caggcggcaa
     8881 gcagcagcaa cgcgccgaga actaacgtaa ttaccgccga catccgcttc agacgcgtct
     8941 cgttttgcat ttgatccgga cggatattca gatagagatc tttaatgatc gtagcggaac
     9001 tttgtagcaa ttgggcgtta attgtcgaca tgatcgcagc cataggtgca gccagaaaga
     9061 tcccggcagc aaacggtggc agcactttta ccattaacgt cgggatcacc agatccggta
     9121 cggtgagatc ggggatcact gcacgaccta acgctccggc taggtgcata ccgaacatca
     9181 ggattgcgac tacaatcgta ccgatgatga tcccccggtg taccgctttg ctgtctttat
     9241 aagagataca gcgcaccgca gtatgcggca ggccaatcac gccaaagcac accagtaccc
     9301 agaacgacgt cataaaggca ggcgacagaa tatcgtcagc gccttgtggc gtaaccagtt
     9361 gcggatcgat ggtttgcaag gtctgtactg cgttgcttaa gccgccagcg gcatgtacca
     9421 cgccaataag cagcacaacg gtgccaatca gcatcacaag cccttgcatg gtgtcgttca
     9481 gcacgctggc gcgaaagccg ccaaaggcgg tatacaacgc aatgctgata ccgaaaatca
     9541 gcagcccggt ttcataagga atacccgccg cggtttccag caggcgcgca ccgccgataa
     9601 actgcacggt cattgcccca acgaacgcaa ccagcaaact caaactcgcc agccacacca
     9661 gaagacgact ctggtagcgg gcaaacagca tgtcgttcag cgtcactgca ttgtagcggc
     9721 gcgcaagaat cgcaaacttc ttgccgagaa taccgagtga aagccagact gcaggaagct
     9781 gaatcatcgc cagcaatacc cagcccagcc cgtatttata agcagctcct ggcccgccga
     9841 taaacgaact ggcactgata taggtcgcgg tgagcgtcat cgccagcaca ataccgccca
     9901 tagagcggct gccgaggaaa tactcattaa ggaaggtgcc ggtgctccgt ttacgcatcg
     9961 cataaaccga gataccgaac accaccacca gataggcgac cagcggtaga attacttcaa
    10021 gctgcatcgt catcctccag tgggatatcg cgatagataa atttcaccat cgcccagcac
    10081 agtccaataa acagtagcgg cgtcaggatg caggccatct caaaccagcg cggaaagccg
    10141 gtaaaaccgg gggcaacgcc aggtaagtaa gcggctacta accaaactgc cagatacaaa
    10201 agggtcagcc ccagcgccca gcgcgcctct ttatgggcct gaacaaaacg agtgtccatt
    10261 ttttgtccct gatgggtgaa gaaagcgagg attgtacctt atgggggttg tcgatcccca
    10321 gtaataaaaa aggccggaaa atccggcctt ttggcgcttt tgcagtctta tttttcctga
    10381 agaccgagtt ttttctccag atagtggatg ttagtgccac catgctggaa gttctcgtca
    10441 ttcatgatgc ggatctgcag atcaacgttg gttttgatac cgtcgatgat cagctcctgc
    10501 agcgcattct tcatgcgggc aatcgccacg tcacggtttt caccgtagca aatcagctta
    10561 ccgatcattg agtcatagta cggcggtacg gtgtagcccg cgtagatatg agactcccaa
    10621 cgtacgccaa aaccgccagg tgcgtggaaa cgggtgattt tgcctgggct tggcaggaag
    10681 gtgttcggat cttcggcgtt gatacgacat tccaccgcat ggccgcgaac gtgaacttct
    10741 tcttgcttga tcgacagcgg ttgaccggca gcgatacgga gctgttcttt gatcaggtca
    10801 acgccagtga tcatttcggt aaccgggtgt tctacctgaa tacgggtgtt catttcgatg
    10861 aaatagaact cgccgttttc gaacagaaac tcgaaagtac ctgcaccgcg atagccgata
    10921 tcaacacacg ctttagcgca acgttcgccg atgtagcgac gcagttccgg ggtaatgccc
    10981 ggtgctggcg cttcttcgac cactttctgg tggcggcgct gcatggagca gtcacgttcc
    11041 gccagataga tagcgttgcc ccgaccgtca gccagtacct gaatctcgac gtggcgagga
    11101 ttttccaggt atttctccat gtaaaccata tcgttgctga aagcagcttt cgcttccgca
    11161 cgggtcatgg agatggattg tgccagttca gcgtcgccgc gcactacgcg cataccgcga
    11221 ccaccgccgc cgccggaggc tttgataatc accggataac caatgcgttt agcaatggca
    11281 cggtttttat ccatatcgtc gcccaacggg ccgtcagaac ccggtacgca agggacgccc
    11341 gcttttttca tcgccgcgat tgcggatact ttgtcgccca tcaggcgaat ggtttctgct
    11401 ttcgggccga tgaagataaa gccggagcgt tcaacctgct cggcaaagtt ggcgttctcg
    11461 gagaggaagc cgtaacccgg atggattgct actgcgccgg tgatttcagc ggcgctgatg
    11521 attgccggga tgttcagata actttttact gacggagcag ggccaataca gaccgtttca
    11581 tctgccagta atacgtgttt tagatcgcga tccgcgctgg agtgcacagc gacagtcttg
    11641 atgcccagtt ctttacaggc acgaagaata cgcaatgcaa tctcgccgcg gttggcaata
    11701 acaattttat ccagcatgtt cgcctcgtta ctcgatgacg accagcggct cgtcaaattc
    11761 taccggttgt ccactttcga ccagaattgc tttcacggta ccggatttgt ccgcttcgat
    11821 ctggttcatc attttcatgg cttcaacgat gcacagggta tcgcccacgt tgactttctg
    11881 acccacttcg atgaacgctt ttgcgtccgg gcttggggtg cggtagaaag taccaaccat
    11941 cggggaacgt acgatgtgac cactgatttc cgctgctgct ggcgcttcca tggaaggaac
    12001 ggtcgccgga gcggctgcgt tagattgagc tggctgctgc atcattggtg cagcgtaagc
    12061 ttgttgcatc acagggaaac ttgcggcagg agctgcacgg ctaatgcgta ctgactcttc
    12121 gccttcagaa atttccagtt cggagatgcc tgattcttca accagctcga tcagtttttt
    12181 aatcttacga atatccatga gtgggttccg tactctttgt ttagtgtgat tgtgacaggc
    12241 gtttcaccgc cgtctgtaaa gcgtatgccc cgacagctgt atgcatagcg ataaattcca
    12301 gcaggccggg acgccggtct attttgcccc agagtatcaa tgttagactt gacggacatt
    12361 gtgcagcgat ctgctgctat cctccggcaa aaaacaaaat ataccttcgt tgcgccaatg
    12421 tcaccttttc cgcagcgcaa aaactgcgtc agggaggacg aggtcgcaca ttataacgat
    12481 ttcgtagcaa ttggcagcta aatactggtc tcatcaggga agataatcaa cagctaacat
    12541 gtaaataacc ttcaacaccg tgtaatttgc aacaagccgc acaattcacg aaattagcgc
    12601 catcatcgac ggaacttctt ataacgtaag gtgaaaagct gtaaagccag ccctgcgaag
    12661 atgaggggct gcaatgagat aatcttcaca gaccacaaat aatgtaggga tgccaggatc
    12721 gcgacaagat agacgacatt acgcagcggt tgctggggat tacccaattt tcactgcatc
    12781 gtctggatag atgtgaatgc taaagcacgc aaaatcaacc gactgacaat tctcagcatt
    12841 aaataggttc gagtggttaa tttgtagtca aataaaatca gattattcac cccatggtac
    12901 cagaaatcag taacaggtta agcgtagtgt cgcccaggca aagcaccata aacctaacag
    12961 gcgacggagg cgtatcaata atggctgttt agaggagtat caatgcgcat caatttgtgg
    13021 tcagtcgaaa tcatgacttt ccagtttgga tgatgttgta caacttcctg accaaggtgt
    13081 tcgacgaaag ctgtccggct aaccgctttt cccgttaacc gattgaataa tgatgtcaaa
    13141 atgagttccc tctcttatta ttccttgcta aatggttagt taaccttcac cagcgtgcga
    13201 ccctggatct ggttattaat gatggcctcg gcaaagttcg gtgcctctga cagagatatc
    13261 tcttttgccg cctgggtata gaatgattcc ggtaaatcgg cgaccagtcg ctgccaggct
    13321 tgtgcgcggc gttctggtgg cgtcattact gaatccaccc cttgcaaacg gacattacgc
    13381 agaataaatg gcatgaccgt ggttggcaga gtaaaaccac ccgccagacc acaggccgcc
    13441 acgcagccgc cgtaattcat ttgcgccagc acttttgcca gcactttgtc gccaacagtg
    13501 tcaattgccc cagcccagac ttgtttttcc agaggacggg attcggcaaa ctcatcacga
    13561 gggagaatac ggctggcacc taaacttttc agatattcat gggtactttc gcgaccggaa
    13621 acggcaacga cctgataacc caacttatgc agcagcgcca cggcggtact gccgacgcca
    13681 ccactggcac ccgtcacgac aatctccccg tcctgcgggc gaacaccggc atcttccagc
    13741 gccatcacac acagcatggc ggtaaaaccg gcagtaccga taatcattgc tttacgcgcg
    13801 tccagccctt gcggcatggc aaccagccag tcacctttca ctcgcgcctg ctccgccagc
    13861 ccaccccagt ggttttcacc aacgccccag ccagtgagta acacctcctg accggcatga
    13921 aaacgcggat cttcgctggt gcgtacagtt ccggcaaaat cgatcccagg aatcatcgga
    13981 aaattacgga tgatttttcc cttaccggta atcgccagcg catctttata gttcaggctc
    14041 gaccagtgaa catcgaccgt gacatcgccc tccggcaggc gactttcgtc cagagtctgt
    14101 actgatgcga gagttttgcc gtcctgctgt tctaaaagta acgcctgcat aagtggtcct
    14161 catgtgcatg atggattgga aaataatttc tgaagattat actcgctaat ggaaaagcaa
    14221 agccgatgaa gcgcaataaa ttgcgagata aatctgattt gctagtatgc ccgcttcctc
    14281 actatcggag ttaacacaag gatgagatta acgacgaaat tttcggcctt tgttacgctg
    14341 ctcaccgggt taacaatttt tgtgactttg ctgggctgtt cgctaagttt ctacaacgca
    14401 attcagtata agtttagtca tcgcgttcag gcggtggcga cggcgattga tacccacctt
    14461 gtgtcgaatg acttcagcac attaaggcca caaattaccg aattaatgat gtcggcagat
    14521 atcgttcgtg tagacctgct ccatggtgat aaacaggttt ataccctggc cagaaatggt
    14581 agttatcgtc cggttggctc cagcgatctg tttcgcgaac tgagtgttcc gttgataaag
    14641 catccgggga tgtcgctgcg tctggtttat caggatccga tgggcaacta tttccattct
    14701 ttaatgacta ccgcgccgct cacgggggcg attggcttta tcattgttat gctcttcctg
    14761 gcggtacgct ggttacaacg gcaacttgcc gggcaagaat tgctggaaac ccgggctact
    14821 cgtatcttaa acggtgagcg tggctctaat gtgttgggaa ccatctatga atggccgccc
    14881 agaaccagca gtgcgctaga tacgctgctt cgtgaaattc agaacgcacg cgaacaacac
    14941 agccgtcttg atacgctgat ccgctcttat gccgcccagg acgtgaaaac cggcctcaat
    15001 aaccgactct ttttcgataa tcagttagca acgttactgg aagatcagga gaaagtaggt
    15061 acccacggga tcgtgatgat gattcgtctg ccggatttca atatgttgag cgacacttgg
    15121 gggcacagcc aggttgaaga acagttcttc actctgacta atctgctgtc gacatttatg
    15181 atgcgctacc ctggcgcact gctggcgcgt taccaccgca gtgattttgc tgcgcagtta
    15241 ccgcaccgga cgttaaaaga ggcagagagc atcgccggtc agttaatcaa agccgtcgat
    15301 accttgccga acaataaaat gctcgatcgc gacgatatga tccacattgg tatctgcgcc
    15361 tggcgtagtg gtcaggatac cgagcaggta atggaacatg cagagtctgc cacgcgtaat
    15421 gcgggattgc agggcggcaa tagctgggct atttacgatg actcgttgcc tgaaaaagga
    15481 cgcggtaatg ttcgctggcg tacgcttatc gagcaaatgc tcagtcgcgg cggcccgcgc
    15541 ctttatcaaa aaccggcggt tactcgcgaa ggtcaggttc atcatcgcga actcatgtgc
    15601 cgcatcttcg atggtaatga agaggttagc tcggcggagt atatgccgat ggtcttgcag
    15661 tttggcttat cggaagagta tgaccgtctg caaatcagcc gtcttattcc actattgcgt
    15721 tactggccag aggaaaatct ggcgattcag gttaccgttg agtcgctgat tcgcccgcgt
    15781 tttcagcgtt ggctgcgcga tacgttaatg caatgtgaaa aatcacaacg aaaacgcata
    15841 attattgaac ttgcagaggc cgatgtaggt caacatatca gtcgtttaca acctgttatt
    15901 cgtttagtga atgctttagg ggtacgggta gccgtcaacc aggctggttt gacgctggta
    15961 agcaccagtt ggatcaaaga acttaatgtt gagttactca agctccatcc ggggctggtc
    16021 agaaacattg agaagcgaac ggagaaccag ctgctggttc aaagcctggt ggaagcctgc
    16081 tccgggacca gcacccacgt ttacgccacc ggcgtgcgtt cgcgaagcga gtggcagacc
    16141 ctgattcagc gcggtgttac aggcgggcaa ggggattttt tcgcgtcctc acagccactt
    16201 gatactaacg tgaaaaaata ttcacaaaga tactcggttt aacctgccgt ttaatccgtt
    16261 ttcacgtaga ataacgcgcg ctgcgtctca tgggagtgtg cttgtctgct cgccagattg
    16321 ttgcagcaca tatgcagatg aatgacctta cgcggttgca aacaggcgag gaatgctgct
    16381 gatgcattaa gcctttctgg actcaggcag agatttgtaa caaaggaaac gaactgcact
    16441 aattttcacc gtagcagatg atttttgcgc cttgtcgctg ctgcgtgtgg ttggtaaagt
    16501 aagcggattt tcttttccgc cccagctttc aggattatcc cttagtatgt tgaaaaaatt
    16561 tcgtggcatg ttttccaatg acttgtccat tgacctgggt actgcgaata ccctcattta
    16621 tgtaaaagga caaggcatcg tattgaatga gccttccgtg gtggccattc gtcaggatcg
    16681 tgccggttca ccgaaaagcg tagctgcagt aggtcatgac gcgaagcaga tgctgggccg
    16741 tacgccgggc aatattgctg ccattcgccc aatgaaagac ggcgttatcg ccgacttctt
    16801 cgtgactgaa aaaatgctcc agcacttcat caaacaagtg cacagcaaca gctttatgcg
    16861 tccaagcccg cgcgttctgg tttgtgtacc ggttggcgcg acccaggttg aacgccgcgc
    16921 aattcgtgaa tccgcgcagg gcgctggtgc ccgtgaagtc ttcctgattg aagaaccgat
    16981 ggctgccgca attggtgctg gcctgccggt ttctgaagcg accggttcta tggtggttga
    17041 tatcggtggt ggtaccactg aagttgctgt tatctccttg aacggcgtgg tttactcctc
    17101 ttccgtgcgc attggtggtg atcgtttcga cgaagctatc atcaactatg tgcgtcgtaa
    17161 ttacggttct ctgatcggtg aagccaccgc agaacgtatc aagcacgaaa tcggttcggc
    17221 ttacccgggc gatgaagtcc gtgaaatcga agttcgtggc cgtaacctgg cagaaggtgt
    17281 tccacgcggt tttaccctga actccaacga aatcctcgaa gcactgcagg aaccgctgac
    17341 cggtattgtg agcgcggtaa tggttgcact ggaacagtgc ccgccggaac tggcttccga
    17401 catctccgag cgcggcatgg tgctcaccgg tggtggcgca ctgctgcgta accttgaccg
    17461 tttgttaatg gaagaaaccg gcattccagt agttgttgct gaagacccgc tgacctgtgt
    17521 ggcgcgcggt ggcggcaaag cgctggaaat gatcgacatg cacggcggcg acctgttcag
    17581 cgaagagtaa tcggatgcag gcaggggaaa tgtctgttta ccctgcctgg tctgatacga
    17641 gaatacgcat aacttatgaa gccaattttt agccgtggcc cgtcgctaca gattcgcctt
    17701 attctggcgg tgctggtggc gctcggcatt attattgccg acagccgcct ggggacgttc
    17761 agtcaaatcc gtacttatat ggataccgcc gtcagtcctt tctactttgt ttccaatgct
    17821 cctcgtgaat tgctggatgg cgtatcgcag acgctggcct cgcgtgacca actagaactt
    17881 gaaaaccggg cgttacgtca ggaactgttg ctgaaaaaca gtgaactgct gatgcttgga
    17941 caatacaaac aggagaacgc gcgtctgcgc gagctgctgg gttccccgct gcgtcaggat
    18001 gagcagaaaa tggtgactca ggttatctcc acggttaacg atccttatag cgatcaagtt
    18061 gttatcgata aaggtagcgt taatggcgtt tatgaaggcc agccggtcat cagcgacaaa
    18121 ggtgttgttg gtcaggtggt ggccgtcgct aaactgacca gtcgcgtgct gctgatttgt
    18181 gatgcgaccc acgcgctgcc aatccaggtg ctgcgcaacg atatccgcgt aattgcagcc
    18241 ggtaacggtt gtacggatga tttgcagctt gagcatctgc cagcgaatac ggatattcgt
    18301 gttggtgatg tgctggtgac ctccggtctg ggcggtcgtt tcccggaagg ctatccggtc
    18361 gcggttgtct cttccgtaaa actcgatacc cagcgcgctt atactgtgat tcaggcgcgt
    18421 ccgactgcag gactgcaacg tttgcgttat ctgctgctgc tgtggggcgc ggatcgtaac
    18481 ggcgctaacc cgatgacgcc ggaagaggtg catcgtgttg ctaatgaacg tctgatgcag
    18541 atgatgccgc aggtattgcc ttcgccagac gcgatggggc caaagttacc tgaaccggca
    18601 acggggatcg ctcagccgac tccgcagcaa ccgacgacag gaaatgcagc tactgcgcct
    18661 gctgcgccca cacagcctgc tgctaatcgc tctccacaaa gggctacgcc gccgcaaagt
    18721 ggtgctcaac cgcctgcgcg tgcgccggga gggcaatagt ggcgagctat cgtagccagg
    18781 gacgttgggt aatctggctc tctttcctca ttgcgctgtt gctgcaaatc atgccctggc
    18841 cggataacct gattgttttc cggccaaact gggtgttact catcttgttg tattggatcc
    18901 tggccttgcc tcatcgcgta aatgtgggca caggttttgt gatgggtgcc atactggatc
    18961 tgatcagcgg ctcgacgctt ggcgtacgcg tattggcgat gagcatcatt gcttacctgg
    19021 tggcgctgaa ataccagctt ttccgcaacc tcgcattatg gcagcaggcg ctggtcgtca
    19081 tgttgctttc gctggtggtg gatattattg ttttctgggc agagttttta gtgattaacg
    19141 tctctttcag accggaagtg ttctggagta gtgtagtcaa tggggtgctc tggccgtgga
    19201 ttttcttgct gatgcgtaaa gtccgtcagc agtttgcagt gcaataaagg tttctatgac
    19261 ttctctgtat ttagcttccg gttctccgcg tcgtcaggag ttacttgcgc aacttggtgt
    19321 gacctttgaa cgtattgtta cgggcattga ggagcagcgt cagccgcagg agagcgcgca
    19381 gcagtatgtt gtgcgtctgg cgcgcgagaa agcacaggcg ggtgtcgcgc aaacggcgca
    19441 ggatctcccg gtgctgggtg cggatactat cgttatcctg aatggagaag tgctggagaa
    19501 accgcgcgac gcagagcatg cggcgcagat gttgcgcaaa ttatcgggtc agacccatca
    19561 ggtgatgacg gcagtggcgt tggccgacag ccagcacatt ctcgattgcc tggtggtcac
    19621 cgatgtgact ttcagaacgt taacagacga agacatcgcg ggctatgtcg ccagcggtga
    19681 accgttagat aaagcaggtg catacggtat tcaggggctg ggtggctgtt ttgtcaggaa
    19741 gataaatggc agctatcacg ccgtagtcgg cttacctctg gttgaaacgt atgaattatt
    19801 aagtaatttt aacgcactgc gtgagaaaag ggataaacat gacggctgaa ttgttagtaa
    19861 acgtaacgcc ttcggaaacg cgagtggcgt atattgatgg cggtattctg caggaaattc
    19921 atattgaacg tgaggcgcga cgcggaatag taggcaatat ctacaagggt cgtgtaagtc
    19981 gtgtacttcc gggtatgcag gcggcttttg tagatattgg gctggataaa gccgcgtttc
    20041 ttcatgcatc cgatatcatg ccgcacaccg aatgtgtggc gggtgaagaa caaaagcaat
    20101 tcacggtgcg cgacatctcg gaactggttc gtcaggggca agatctgatg gtgcaggtgg
    20161 tgaaagatcc gcttggcact aaaggtgcgc gcctgaccac cgatatcacg ctgccttctc
    20221 gctatctggt ctttatgcca ggggcttctc acgttggggt ttcccaacgt attgaaagcg
    20281 aatcagaacg tgaacgcctg aaaaaagtgg tcgcagagta ttgcgacgag cagggcgggt
    20341 ttatcatccg taccgcagcg gaaggggttg gcgaggctga actggcctcc gatgccgctt
    20401 atctgaaacg cgtctggacc aaagttatgg agcgtaaaaa gcgcccgcag acccgttatc
    20461 agctgtacgg cgaactggcg ctggctcagc gtgttctgcg tgatttcgcc gacgccgaac
    20521 tggaccgcat tcgcgttgac tcacgcctga cttacgaagc attacttgag ttcacctcgg
    20581 agtacattcc cgagatgacc agcaagctgg agcattacac cggacgccag ccgattttcg
    20641 atctctttga tgtcgaaaac gaaatccagc gagcgctgga acgcaaagta gaactgaaat
    20701 ccggtggtta tctcattatc gaccagaccg aagcgatgac caccgtggac atcaataccg
    20761 gagcgtttgt cggtcatcgc aatctggacg acaccatttt caataccaat attgaagcga
    20821 cgcaggctat cgctcgccag ttacggttgc gtaatctggg cgggattatc attattgatt
    20881 tcatcgatat gaataatgaa gatcaccgcc gccgcgtgct gcactcgctg gagcaggcgt
    20941 tgagcaaaga ccgggtgaaa accagcgtta atggtttttc ggcgctgggg ctggtggaga
    21001 tgacgcgtaa acgcacccgc gaaagcattg agcacgtact gtgtaacgaa tgcccaacct
    21061 gccacggtcg cggaacggtg aaaaccgtgg aaacggtatg ctatgaaatc atgcgcgaga
    21121 ttgttcgtgt ccaccatgct tacgactccg accgtttcct ggtctatgct tctccggcag
    21181 tagccgaagc cttgaaaggc gaagagtcac actcgctggc ggaagtggaa attttcgttg
    21241 gcaaacaggt taaagtacaa attgaaccgc tctataacca ggagcagttt gacgtcgtaa
    21301 tgatgtaaac agatgctggg ccgccatccg gcaaagggtt tttgagtcac atttttagca
    21361 gacaaggagt gacgggtgag gcgattgccg gggattttac tgcttactgg agccgcgctc
    21421 gttgtgatcg ctgccctgct ggttagcggc ctgcgtattg ctttaccgca tcttgacgcc
    21481 tggcgtccgg aaatcctcaa caaaatagaa tccgcgactg gcatgccggt agaagccagt
    21541 cagctctcag ccagctggca gaattttggc ccgacgcttg aagcacacga catccgtgca
    21601 gaactaaaag atggcggcga attttcggtt aaacgcgtta ctctggcgct ggatgtctgg
    21661 cagagcctgt tacatatgcg ctggcagttt cgcgacctca ctttctggca gctgcgcttt
    21721 cgcaccaaca ctcctatcac cagcggtggt agcgatgaca gtctggaagc cagtcacatc
    21781 agcgatctgt ttcttcgtca atttgaccat ttcgatctcc gcgacagtga agtcagtttc
    21841 ctgacgccat ccggtcagcg tgccgagctg gcgatcccac aactcacctg gctgaacgat
    21901 ccacgtcgac accgtgcgga aggcctggta agcctctcca gccttaccgg acagcacggc
    21961 gtgatgcagg tgcgcatgga tttgcgcgat gatgaggggt tgttaagcaa tggtcgcgtc
    22021 tggctacagg cggatgacat cgacctgaag ccgtggctcg gtaaatggat gcaggacaat
    22081 attgctctgg aaacggcaca gttctccctt gaaggctgga tgacgatcga caaaggcgat
    22141 gtaaccggcg gtgacgtctg gctgaaacag ggcggtgcca gctggttggg cgagaagcaa
    22201 acgcatacgc tgtcggtgga taatctgacc gcgcatatta cgcgtgaaaa tccgggctgg
    22261 cagttctcta ttcccgatac acggatcacg atggacggca aaccctggcc gagcggagca
    22321 ttgacgctgg cctggatacc ggaacaggac gttggcggca aagacaataa acgcagtgac
    22381 gaactccgga ttcgcgccag taatctggag ctggcaggcc tggagggcat acgcccgctg
    22441 gccgcgaaac tttcacctgc actgggtgat gtttggcgct ccacacaacc gagcggcaag
    22501 attaacactc tggcgctgga tatcccgctt caggcggcag acaagacccg ttttcaggca
    22561 tcgtggagcg atctggcctg gaagcaatgg aaattattac cgggtgcgga acacttctcc
    22621 gggacgcttt ccggcagcgt tgaaaatggt ttgcttaccg cgtcgatgaa gcaggcaaag
    22681 atgccttacg aaacggtatt ccgtgcgcca ctggaaatcg ccgacggcca ggcaactata
    22741 agctggctga acaatgacaa aggtttccag ctggatgggc gtaatattga cgttaaagcc
    22801 aaagccgtcc atgcgcgcgg cggttttcgt tacctgcaac ctgctaacga tgaaccctgg
    22861 ctgggtattc tggctggcat cagtaccgat gatggttcac aagcctggca ctatttcccg
    22921 gaaaacttga tgggtaaaga cctggttgat tacttaagtg gcgcgattca gggcggtgaa
    22981 gcggataacg cgacgctggt ttatggtggc aatccgcaac tcttccccta taaacacaac
    23041 gaaggtcagt ttgaagtgct ggtgccgctg cgcaacgcga agtttgcctt ccagccggac
    23101 tggcctgcat taactaacct tgatattgaa ctggacttta ttaacgacgg tttatggatg
    23161 aaaaccgatg gcgttaatct gggcggcgtg cgcgcgagta atctcaccgc agtgatccct
    23221 gactactcga aagaaaaact gctgattgac gctgacatta aaggtccggg taaagccgtt
    23281 ggcccttact ttgatgagac accgctgaaa gattctctgg gtgcgaccct gcaagaactc
    23341 cagctcgacg gcgatgtgaa tgctcgctta catcttgata tcccgctgaa cggcgagctg
    23401 gtaaccgcga aaggtgaagt gacgctgcgt aataacagtc tgtttatcaa accactcgac
    23461 agcaccctga aaaatttgag cggtaaattc agctttatca atggcgatct gcaaagtgaa
    23521 ccactgacag caagctggtt taatcagccg ttgaacgtgg atttttccac caaagaaggg
    23581 gcaaaagcct accaggttgc ggtgaatctc aacggtaact ggcaaccggc gaaaaccggc
    23641 gttctgcctg aagcggtgaa cgaagcattg agtggcagcg tggcgtggga tggtaaagtg
    23701 ggcattgatc tgccttatca tgctggcgcg acctataacg tagagctgaa cggcgatctg
    23761 aataatgtga gtagtcactt accttcaccg ttagccaaac ctgcgggtga accactagcg
    23821 gtaaacgtta aggttgatgg caatctcaac agctttgaat taaccggaca ggctggtgcg
    23881 gataaccatt tcaatagccg ctggttgctt ggtcaaaagc tgacgctcga tcgtgctatt
    23941 tgggcggcag acagtaaaac gctcccgccg ttgccggaac aaagtggcgt tgaactcaat
    24001 atgccgccga tgaatggtgc cgagtggctg gccctgttcc agaaaggcgc tgcggagagt
    24061 gtcggtggtg cagcgagttt cccacaatac ataacgttac gtacgcctat gttgtcactg
    24121 ggaaatcagc aatggaataa cctgagtatt gtttcgcaac cgacggcaaa tggcaccctg
    24181 gttgaggcgc aagggcgtga aatcaacgcc acgctagcga tgcgtaataa cgcgccgtgg
    24241 ctggcgaata tcaaatatct ttattacaac ccgagcgtgg cgaaaactcg tggtgattca
    24301 acgccgtcat cacctttccc gacaacggag cgcattaact tccgtggctg gccggacgca
    24361 caaatacgat gcacagagtg ctggttctgg gggcaaaaat tcggtcgcat tgacagtgat
    24421 atcaccattt ctggcgatac gttaacgctg accaatggac tgattgatac tggtttctcg
    24481 cggcttactg ccgatggtga atgggttaat aatccgggga atgaacgtac ctcgctgaaa
    24541 ggaaaactgc gcgggcagaa aattgatgcc gccgcagaat tttttggtgt cacgacgccc
    24601 atacgccagt cgtcatttaa tgtggattac gatttacact ggcgcaaagc accctggcag
    24661 ccagatgaag cgacgttgaa tggcatcatt catactcaac tgggtaaagg cgaaattacc
    24721 gaaatcaata ccggacatgc cgggcaattg ctgcgcttat tgagcgtaga tgccctgatg
    24781 cgtaagctgc gttttgattt cagagacact tttggcgaag ggttctattt tgactccatt
    24841 cgcagcaccg cgtggattaa agacggggtt atgcacaccg acgacacgct ggtggatggc
    24901 ctggaggcgg atatcgccat gaaagggtcg gtaaatctgg tacgtcgcga cctgaatatg
    24961 gaagcggttg tcgcaccaga gatttctgcg acggtgggcg tggctgcggc ttttgcggtt
    25021 aaccccattg ttggcgcggc agtttttgcc gctagtaaag tgctggggcc gctgtggagc
    25081 aaagtctcca ttttgcgcta tcacatttcg ggtccgctgg acgatccgca aatcaacgaa
    25141 gtgttgcgcc aaccgcgtaa agaaaaagcg caatgatttg acgagggcgc gtaattgccc
    25201 caatctcata tgataatcgt tgccaaaggc caacgagcca gaacataacc gtaggccgga
    25261 tagggcgttc atgccgcatc cggcagccgt aaaaaatcct ctactgcagt aactaacgag
    25321 tagcaaaaac gatgagtctg aacctggtaa gtgaacaatt gctagcggcg aacagcctga
    25381 aacatcagga cttgttcgcg atcctcggtc aactggccga acgtcgcctt gattatggcg
    25441 atctctattt tcagtcgagc tatcacgaat cctgggtttt agaagaccgc attattaaag
    25501 atggttctta caacatcgat cagggcgttg gcgtgcgtgc aatcagcggt gaaaaaaccg
    25561 gatttgctta cgctgaccaa atcagcctgc tggcgctgga acagagtgcg caagcggcgc
    25621 gcaccatcgt ccgtgatagt ggtgatggca aagtacagac gctgggcgcg gtagagcata
    25681 gcccgttgta tacctcggta gatccgctgc aaagcatgag ccgtgaagag aagctggata
    25741 tcctgcgtcg cgtcgataag gttgctcgcg aagcggacaa gcgcgtacag gaagtgactg
    25801 ccagcctcag tggtgtctat gaattaattc tggttgcggc caccgacggc acgctagcgg
    25861 cggatgtccg tccgctggtg cgtctttccg tgagcgttct ggtcgaagaa gatggcaaac
    25921 gcgaacgcgg tgccagtggc ggcggcggtc gttttggtta tgagttcttc cttgccgatc
    25981 ttgacggcga agtccgtgcg gatgcatggg caaaagaagc agtgcgtatg gcgctggtca
    26041 atctttctgc cgttgctgca ccagcgggca ccatgccggt agtacttggc gcaggttggc
    26101 cgggcgtgct gttgcatgaa gcggtaggtc acggtctgga aggcgacttc aaccgccgcg
    26161 gcacttcagt atttagtggg caggtcgggg agctggtggc ttcagaactg tgtaccgtgg
    26221 ttgatgacgg cacgatggtc gatcgccgtg gttcggtggc gattgatgac gaaggtacgc
    26281 caggccagta caacgtgctg attgagaacg gcattctgaa aggctacatg caggataaac
    26341 tcaacgcgcg tttgatgggg atgacgccga ctggcaacgg tcgccgtgaa tcctacgccc
    26401 atctgcccat gccgcgtatg actaacacct atatgctgcc gggtaaatcg accccgcagg
    26461 aaattattga atccgttgag tacggtatct atgcaccgaa ctttggtggc ggtcaggtgg
    26521 atatcacctc cggcaaattc gttttctcca cttcagaagc atatctgatt gaaaacggta
    26581 aagtaacgaa gccggtgaaa ggcgcaacgt tgattggttc cggtatcgaa accatgcagc
    26641 agatttcgat ggttggcaac gacctgaaac tggataacgg cgtgggtgtc tgcggtaaag
    26701 aagggcaaag tttgccggtt ggcgtgggcc agccaacgct gaaagttgat aacctgactg
    26761 ttggcggtac tgcgtaataa tttcattatt ttcagaagga taattaatct ttctacgtgc
    26821 acgaacggtc ccctcgctcc tctggggtta gggtgagggg aacccgttgg cacaggtttg
    26881 tagaacgtaa cagtacaata tgaattactt ctctttcccg cgcccgtgca tctcctgaaa
    26941 caatttaccg acctcaacaa aataatccgt cagcgagttg atcacgacct gtaccttcag
    27001 cggcagctta tctttttcgg tatataacgc ataaaccggg cgtggatctg actgataacg
    27061 cggcagcagg atctccagct caccgcgatt gatctcgttg atcacccaca tcagcggcac
    27121 gtaggcgatc ccggcacccg ccgtcagcca gcgcaccagc gtcatcggat cattagtcac
    27181 aaatcttcct tgtgggatca ggcgagtcga gatcccttcc ggtgcgatca gttcaaattc
    27241 attgtcgggc cgcacgctgt attcaagcca tgaatgacta ctcaaatcgg cgggtttttc
    27301 cggtatgccg tattgtgtga gatagctttt cgcggcgcac accaccatcg gcatcgcgcc
    27361 cagacggcgg gaaaacaggc tggaattccg gtaaccagat tgacgctcaa acctgggtat
    27421 tctttcagca tttttgctgt cagcccggcg agaacatttt gtgccatagt tgaagaacag
    27481 ccaatgcgca gcgtcccgat gggggtgtta ttgaaggcat acagttgctc atgaacatcc
    27541 tgcacttcat gaagcatccg acggcagccc tggtagtaaa ttctgccggc ttcggtcagg
    27601 ccaatgctgc gtgtgctacg gtttaacagc tttacctgca actcatcttc cagttttgac
    27661 accgtctgac tgatggacga aacgctcatc tgtagctgtc tggcggcggc ggtaaaagag
    27721 ccaaattcaa ctactttggc aaacaccgac atgcgtttta gtcgttccat tattcactct
    27781 gacttaaaag tgatttagat cacataatat agataacagc ataacagtta cgctaatata
    27841 ttaaatatca atctacagca atgttgctct cgcccggctt tccatgccct tctctgcggc
    27901 gacagatgct gaaaataata acgcctgctc tctctttaca accaaggtca acatgagtct
    27961 gtttcccgtt atcgtggtgt ttggactgtc cttcccaccg atattttttg aattgctttt
    28021 atcactggcg attttctggc tggtgcgccg ggtacttgtg ccaacaggta tctacgactt
    28081 tgtctggcat ccggcgttgt tcaacaccgc gctctattgc tgcttgtttt atttgatatc
    28141 gcgactgttc gtttgaggtt gaagtgaaaa cactaataag aaaattctcc cgtacggcca
    28201 tcacggtcgt attagtcatt ctagccttca tcgcaatttt taatgcctgg gtctattaca
    28261 ccgaatcccc ctggacgcgt gacgcgcgct ttagcgctga cgtcgttgcg atcgcgccgg
    28321 acgtttctgg actcattacc caggtgaatg ttcatgataa ccagctggtg aaaaaaggac
    28381 agatactgtt caccatcgac cagccgcgct atcaaaaggc gcttgaggaa gcgcaagccg
    28441 atgttgctta ttatcaggta ctggcacagg agaaacgcca ggaggccgga cgtcgtaacc
    28501 gtctcggtgt gcaggcgatg tctcgcgaag agatcgacca ggccaacaac gtactacaaa
    28561 cggttctgca tcagttagcg aaagcgcagg cgacccgcga tctggcaaaa ctggatcttg
    28621 aacgcacggt gatccgcgcg ccagcagatg gctgggtgac caacctcaac gtctataccg
    28681 gtgagtttat tactcgagga tcaacggcgg ttgcgctggt gaaacagaac tccttctatg
    28741 tactggccta tatggaagaa actaagctgg aaggggggcg tccggggtat cgtgcagaga
    28801 tcacgccgct tggcagtaac aaagtgctga aagggactgt tgatagtgtt gccgcagggg
    28861 tcaccaacgc cagcagcacg cgtgacgaca aagggatggc gactatagac tctaatcttg
    28921 aatgggtgcg tcttgcgcaa cgtgttccgg ttcgtattcg tctcgacaac cagcaagaga
    28981 acatctggcc tgcgggcacc actgctacag tggtggtcac tggcaaacaa gatcgcgacg
    29041 aaagccagga ttcgttcttc cgtaaaatgg cccatcgcct gcgtgagttt ggttaatcac
    29101 gatgggtatt ttctccattg ctaaccaaca tattcgcttt gcggtaaaac tggcgaccgc
    29161 cattgtactg gcgctgtttg ttggctttca cttccagctg gaaacgccac gctgggcggt
    29221 actgacagcg gcgattgttg ccgccggtcc ggcctttgct gcgggaggtg aaccgtattc
    29281 tggcgctatt cgctatcgtg gctttttgcg catcatcggc acatttattg gctgtattgc
    29341 cggactggtg atcatcattg cgatgatccg cgcaccatta ttgatgattc tggtgtgctg
    29401 tatctgggcc ggtttttgta cctggatatc ctcgctggta cgaatagaaa actcgtacgc
    29461 gtgggggctg gccggttata ccgcgctgat cattgtgatc accattcagc cggaaccatt
    29521 gcttacgccg cagtttgccg tcgaacgttg tagcgagatc gttatcggta ttgtgtgtgc
    29581 gattatggcg gatttgctct tttctccgcg atcgatcaaa caagaagtag atcgagagct
    29641 ggaaagtttg ctggtcgcgc aatatcaatt aatgcaactc tgtatcaagc atggcgatgg
    29701 tgaagttgtc gataaagcct ggggcgacct ggtgcgacgc accacggcac tacaaggcat
    29761 gcgcagcaac ctgaatatgg aatcttcccg ctgggcgcgg gccaatcgac gtttaaaagc
    29821 gatcaatacg ctatcgctga cgctgattac ccaatcctgc gaaacttatc ttattcagaa
    29881 tacgcgcccg gaattgatca ctgatacttt ccgcgaattt tttgacacgc cggtagaaac
    29941 cgcgcaggac gtccacaagc agctcaaacg cctgcggaga gttatcgcct ggaccgggga
    30001 acgggaaacg cctgtcacca tttatagctg ggtcgcggcg gcaacgcgtt atcagcttct
    30061 caagcgcggc gttatcagta acacaaaaat caacgccacc gaagaagaga tcctgcaagg
    30121 cgaaccggaa gtaaaagtag agtcagccga acgtcatcat gcaatggtta acttctggcg
    30181 aaccacactt tcctgcattc tgggcacgct tttctggctg tggacgggct ggacttccgg
    30241 cagtggtgca atggtgatga ttgcggtcgt gacgtcactg gcaatgcgtt tgccgaatcc
    30301 acgcatggtg gcgatcgact ttatctatgg gacgctggcc gcgctgccgt tagggctgct
    30361 ctactttttg gtgattatcc ctaataccca acagagcatg ttgctgctgt gtattagcct
    30421 ggcagtgctg ggattcttcc tcggtataga agtacagaaa cggcgactgg gctcgatggg
    30481 ggcactggcc agcaccataa atattatcgt gctggataac ccgatgactt tccatttcag
    30541 tcagtttctc gacagcgcat tagggcaaat cgtcggctgt gtgctcgcgt tcaccgttat
    30601 tttgctggtg cgggataaat cgcgcgacag gactggacgt gtactgctta atcagtttgt
    30661 ttttgccgct gtttccgcga tgactaccaa tgtggcacgt cgtaaagaga accacctccc
    30721 ggcactttat cagcagctgt ttttgctgat gaataagttc ccaggggatt tgccgaaatt
    30781 tcgcctggcg ctgacgatga ttatcgcgca ccagcgcctg cgtgatgcgc cgatcccggt
    30841 taacgaggat ttatcggcgt ttcaccgaca aatgcgccgc acagcagacc atgtgatatc
    30901 tgcccgtagc gatgataaac gtcgtcggta ctttggccag ttgctggaag aactggaaat
    30961 ctaccaggaa aagctacgca tctggcaagc gccaccgcag gtgacggaac cggttcatcg
    31021 gctggcgggg atgctccata agtatcaaca tgcgttgacc gatagttaag tcaaaaccga
    31081 cgccaaaagc gtcggttttt tcatggctat acttagcgat gcacggcaga actcgccgcg
    31141 aaacgtgacg gtggcaacag atgaatattt atacctttga ttttgatgag attgagagtc
    31201 aggaggattt ttatcgtgac tttagccaaa cctttggtct ggctaaagat aaggtacgcg
    31261 atctcgactc actatgggat gtgttaatga acgatgtcct gccgctacca cttgagattg
    31321 aatttgttca tctgggagag aaaacgcgtc gccgttttgg cgcgttaata ttgctgtttg
    31381 atgaggcaga ggaagagctg gaagggcatt tgcgttttaa tgttcgtcat tagcgcaaaa
    31441 aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt atttgtacag
    31501 ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt aggccgttgc
    31561 gcctttctct tccgcttttt tgttcagcat ttcacgcata tccattggcg aagacgccac
    31621 accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag tatcaatgga
    31681 gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag ctacagtggt
    31741 tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg tttcgtgatc
    31801 catatcacaa aatcaagcat acactaatta ttgaactaat taataccaca ctaactgttt
    31861 cagttgatac taagtgttaa aaatgtgaat tttaataaca aaaaagaatt aaaaacacgg
    31921 ttatttattg ttaattctag tattttcaat tagataatga attttacata atgtgctttt
    31981 tttaccgtat gtattcattg tgtgaatgat atgtcgcggt aaaacgcact attcgttaaa
    32041 ttaatcgcag gggaaagcag ggggttgaga ggaataagca acattttccc cgccgccaga
    32101 agcgacgggg cagagattaa agctcctggt cgaacagctc taaaatcgct tcgtacaggt
    32161 ctttgacggt gaaaccgtta gcaggggtgg taaagatggt gtcatcgcca gcgatggtgc
    32221 ccagaatacc ttctgctttg cccagtgagt ccagcaggcg agcaattaac tgcgccgcgc
    32281 cagggctggt atgaatcacg acaactgcat cgttgtagtc gatatccagc accagattct
    32341 tcaatggact ggaggtggtt ggtacaccca gttcagctgg caggcagtaa accatttcca
    32401 ttttggcatt gcgtgtacgt acagcaccaa acttggtcaa catccgcgag actttagact
    32461 gattaatatt gtcaaagcct tgctcctgca acgcggcgac gatttcgccc tgggagctaa
    32521 atttctcttc tttaagtaat gctttaaatg ctttaactag ttcttcttgc ttagccgagc
    32581 ttcgcataag tcacccgata tggtggttga tacaacatta ttgtgcatac agatgaattt
    32641 ttatgcaaac agtcagccct gaagaaggct gaaataatgt tatgaaagag cgggatttta
    32701 tcaaatttcg ttattgagaa acatgcctgc gtcacggcat gcaaattctg tttaaaagta
    32761 aattaattgt tatcaaattg atgttgtttt ggctgaacgg tagggtatat tgtcaccacc
    32821 tgttggaatg ttgcgctaat gcataagcga ctgttaatta cgtaagttag gttcctgatt
    32881 acggcaatta aatgcataaa cgctaaactt gcgtgactac acattcttga gatgtggtca
    32941 ttgtaaacgg caattttgtg gattaaggtc gcggcagcgg agcaacatat cttagtttat
    33001 caatataata aggagtttag gatgaaagtc gcagtcctcg gcgctgctgg cggtattggc
    33061 caggcgcttg cactactgtt aaaaacccaa ctgccttcag gttcagaact ctctctgtat
    33121 gatatcgctc cagtgactcc cggtgtggct gtcgatctga gccatatccc tactgctgtg
    33181 aaaatcaaag gtttttctgg tgaagatgcg actccggcgc tggaaggcgc agatgtcgtt
    33241 cttatctctg caggtgtagc gcgtaaaccg ggtatggatc gttccgacct gtttaacgtt
    33301 aacgccggca tcgtgaaaaa cctggtacag caagttgcga aaacctgccc gaaagcgtgc
    33361 attggtatta tcactaaccc ggttaacacc acagttgcga ttgctgctga agtgctgaaa
    33421 aaagccggtg tttatgacaa aaacaaactg ttcggcgtta ccacgctgga catcattcgt
    33481 tccaacactt ttgttgcgga actgaaaggc aaacagccag gcgaagttga agtgccggtt
    33541 attggcggtc actctggtgt taccattctg ccgctgctgt cacaggttcc tggcgttagt
    33601 tttaccgagc aggaagtggc tgatctgacc aaacgcatcc agaacgcggg tactgaagtg
    33661 gttgaagcga aagccggtgg cgggtctgca accctgtcta tgggccaggc agctgcacgt
    33721 tttggtctgt ctctggttcg tgcactgcag ggcgaacaag gcgttgtcga atgtgcctac
    33781 gttgaaggcg atggtcagta cgcccgtttc ttctctcaac cgctgctgct gggtaaaaac
    33841 ggcgtggaag agcgtaaatc tatcggtacc ctgagcgcat ttgaacagaa cgcgctggaa
    33901 ggtatgctgg atacactcaa gaaagatatc gccttgggcg aagagttcgt taataagtaa
    33961 ttgattagcg aataataaaa aaccggagca cagac
//
LOCUS       DAETQN010000036        33015 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.036, whole genome shotgun sequence.
ACCESSION   DAETQN010000036 DAETQN010000000
VERSION     DAETQN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.036"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="K0W68_001711"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="K0W68_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HBI3073294.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="K0W68_001712"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="K0W68_001712"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBI3073295.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="K0W68_001713"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="K0W68_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBI3073296.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3697..5964
                     /gene="adiA"
                     /locus_tag="K0W68_001714"
     CDS             3697..5964
                     /gene="adiA"
                     /locus_tag="K0W68_001714"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBI3073297.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            6289..7050
                     /gene="adiY"
                     /locus_tag="K0W68_001715"
     CDS             6289..7050
                     /gene="adiY"
                     /locus_tag="K0W68_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBI3073298.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            7187..8524
                     /gene="adiC"
                     /locus_tag="K0W68_001716"
     CDS             7187..8524
                     /gene="adiC"
                     /locus_tag="K0W68_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBI3073299.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            8628..10271
                     /gene="eptA"
                     /locus_tag="K0W68_001717"
     CDS             8628..10271
                     /gene="eptA"
                     /locus_tag="K0W68_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBI3073300.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            10268..10936
                     /gene="basR"
                     /locus_tag="K0W68_001718"
     CDS             10268..10936
                     /gene="basR"
                     /locus_tag="K0W68_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBI3073301.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            10937..12037
                     /gene="pmrB"
                     /locus_tag="K0W68_001719"
     CDS             10937..12037
                     /gene="pmrB"
                     /locus_tag="K0W68_001719"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBI3073302.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="K0W68_001720"
     CDS             complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="K0W68_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBI3073303.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(12214..13716)
                     /gene="proP"
                     /locus_tag="K0W68_001721"
     CDS             complement(12214..13716)
                     /gene="proP"
                     /locus_tag="K0W68_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBI3073304.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(13980..14846)
                     /locus_tag="K0W68_001722"
     CDS             complement(13980..14846)
                     /locus_tag="K0W68_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBI3073305.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="K0W68_001723"
     CDS             complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="K0W68_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBI3073306.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            17484..17819
                     /locus_tag="K0W68_001724"
     CDS             17484..17819
                     /locus_tag="K0W68_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBI3073307.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            17977..18420
                     /gene="yjdN"
                     /locus_tag="K0W68_001725"
                     /pseudo
     CDS             17977..18420
                     /gene="yjdN"
                     /locus_tag="K0W68_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            18553..19341
                     /gene="phnC"
                     /locus_tag="K0W68_001726"
     CDS             18553..19341
                     /gene="phnC"
                     /locus_tag="K0W68_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBI3073308.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            19366..20382
                     /gene="phnD"
                     /locus_tag="K0W68_001727"
     CDS             19366..20382
                     /gene="phnD"
                     /locus_tag="K0W68_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3073309.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            20508..21287
                     /gene="phnE"
                     /locus_tag="K0W68_001728"
     CDS             20508..21287
                     /gene="phnE"
                     /locus_tag="K0W68_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HBI3073310.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            21308..22033
                     /gene="phnF"
                     /locus_tag="K0W68_001729"
     CDS             21308..22033
                     /gene="phnF"
                     /locus_tag="K0W68_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HBI3073311.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            22034..22486
                     /gene="phnG"
                     /locus_tag="K0W68_001730"
     CDS             22034..22486
                     /gene="phnG"
                     /locus_tag="K0W68_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBI3073312.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            22483..23067
                     /gene="phnH"
                     /locus_tag="K0W68_001731"
     CDS             22483..23067
                     /gene="phnH"
                     /locus_tag="K0W68_001731"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBI3073313.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            23067..24131
                     /gene="phnI"
                     /locus_tag="K0W68_001732"
     CDS             23067..24131
                     /gene="phnI"
                     /locus_tag="K0W68_001732"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HBI3073314.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            24124..24969
                     /gene="phnJ"
                     /locus_tag="K0W68_001733"
     CDS             24124..24969
                     /gene="phnJ"
                     /locus_tag="K0W68_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBI3073315.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            24966..25724
                     /gene="phnK"
                     /locus_tag="K0W68_001734"
     CDS             24966..25724
                     /gene="phnK"
                     /locus_tag="K0W68_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HBI3073316.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            25835..26515
                     /gene="phnL"
                     /locus_tag="K0W68_001735"
     CDS             25835..26515
                     /gene="phnL"
                     /locus_tag="K0W68_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HBI3073317.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            26512..27648
                     /gene="phnM"
                     /locus_tag="K0W68_001736"
     CDS             26512..27648
                     /gene="phnM"
                     /locus_tag="K0W68_001736"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBI3073318.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            27648..28205
                     /gene="phnN"
                     /locus_tag="K0W68_001737"
     CDS             27648..28205
                     /gene="phnN"
                     /locus_tag="K0W68_001737"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HBI3073319.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            28192..28626
                     /gene="phnO"
                     /locus_tag="K0W68_001738"
     CDS             28192..28626
                     /gene="phnO"
                     /locus_tag="K0W68_001738"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HBI3073320.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            28628..29386
                     /gene="phnP"
                     /locus_tag="K0W68_001739"
     CDS             28628..29386
                     /gene="phnP"
                     /locus_tag="K0W68_001739"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HBI3073321.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(29533..29862)
                     /gene="yjdP"
                     /locus_tag="K0W68_001740"
     CDS             complement(29533..29862)
                     /gene="yjdP"
                     /locus_tag="K0W68_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HBI3073322.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(29931..30248)
                     /locus_tag="K0W68_001741"
     CDS             complement(29931..30248)
                     /locus_tag="K0W68_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HBI3073323.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            30501..32486
                     /gene="yjcS"
                     /locus_tag="K0W68_001742"
     CDS             30501..32486
                     /gene="yjcS"
                     /locus_tag="K0W68_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HBI3073324.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            32695..32970
                     /locus_tag="K0W68_001743"
     CDS             32695..32970
                     /locus_tag="K0W68_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073325.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         8025 a         9109 c         8460 g         7421 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcagatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaataat ttaaaataat cacataacgc attgtttata aaaataaata
     3601 tttcaaaaat gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc
     3661 cgcaacgaag attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc
     3721 gagtttctcc atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta
     3781 agccagcaaa atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc
     3841 tcttcaaacg aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa
     3901 catcaaaacg tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc
     3961 ttcctgttgg gcgatcggga aaaagccctc gccgcaatgg atcgcgacct gctggagctt
     4021 gtcgatgaat tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgctgtt
     4081 gccgcgatga cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa
     4141 tatagcgaca tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt
     4201 accaaaacac ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc
     4261 gatatgggca tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc
     4321 gaaagcgaaa aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc
     4381 ggtacttccg gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg
     4441 gtcgttgacc gtaactgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa
     4501 ccggtctaca tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag
     4561 gaaatgcaac ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa
     4621 gccgggcaaa aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat
     4681 aacgctaaag aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa
     4741 gcctggtacg gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc
     4801 gaacctggcg atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg
     4861 aatgcgcttt cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc
     4921 tcccgcttca accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc
     4981 gcatccaacg acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacgcag
     5041 gaagtgatcg acgaagcggt tgatttccgt caggcgatgg cgcggctgta taaagagttc
     5101 accgctgacg gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa
     5161 accggcaaaa cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac
     5221 tgctgggtaa tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg
     5281 agtatgctcg acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa
     5341 ctggaagaaa ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt
     5401 gtgcctaccc gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt
     5461 gggaaatggg gaactctggt taacaccctt tgctccttca aacgccacta tgacgccaac
     5521 acaccgctgg cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac
     5581 atggggattc acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc
     5641 gcacggttga acgaagccta ttccggcctg tcggtggcgg aaatcacccc gcgtgaagcg
     5701 tacaacgcga ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc
     5761 atcgcggcaa actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa
     5821 aacttcggcg ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac
     5881 caccatttcc ctggatttga acacgaaact gaagggactg aaattattga cggtatttac
     5941 cacgttatgt gtgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac
     6001 attacgccgc ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca
     6061 aacaaaaatg tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta
     6121 tcctgtaaaa aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact
     6181 cgttattttt atgcatgttt atattcatca tacaattata taaccatttc ccggtatcgc
     6241 tttgctttag cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc
     6301 agcgaccaac cttgtattgt tttattgact gaaaaagatg tctggataag ggtgaatggg
     6361 aaagaaccta ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt
     6421 atcgacgtct cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa
     6481 gattacctcc ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct
     6541 acgcccatac tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac
     6601 agcatgatgc ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg
     6661 gtgctatccc gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt
     6721 aattgtgtaa gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat
     6781 cttagtatgg tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagctgaaa
     6841 agcgaaaaca ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat
     6901 gaattaatga tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt
     6961 acgtcgtact ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt
     7021 agtcagcaca gagaacgaac tgtcgcctga tttttaacct taacgaagag ctatattaat
     7081 aacggcatca gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata
     7141 tgacgtaatt tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc
     7201 tgatgctcac aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg
     7261 gtcaggtgtt tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg
     7321 atggttggtg acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt
     7381 cctcgatcca agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct
     7441 cggttatcaa accaacgtcc tctactggct ggcctgctgg atcggcaata tcgctatggt
     7501 ggtcattggc gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt
     7561 aaccatcacc tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa
     7621 aatgatcacc cgtgtgcagg ccgttgccac cgtgctggcg ctgatcccca tcgtcgggat
     7681 tgccgtattt ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag
     7741 cggcctgggc accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat
     7801 cggtgtggaa agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc
     7861 tatcgccacc attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc
     7921 gattatgggg atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc
     7981 cgcacggatg gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg
     8041 ttgcttaggt tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc
     8101 cgatgacgga ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc
     8161 ggggctgatt atcgtcggta ttttgatgac catcttccag ctcagtagca tttcgccaaa
     8221 cgcaaccaaa gagttcggtc tggtatcttc cgtgtcggtc atctttacac tggtgccata
     8281 tctttacacc tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc
     8341 ggcatatctg gctgttacta ccattgcctt cctctactgc atctgggccg tggtggggtc
     8401 cggggcgaaa gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc
     8461 gctgaattac aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga
     8521 ttaattcccc ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa
     8581 ttttgctttg cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc
     8641 tactaaaaag cccctctttg aatttactcg cctggctatt gttggccgct ttttatatct
     8701 ctatctgcct gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc
     8761 tgcataacgt actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg
     8821 tcctgacact aagctctttc ttatggctta atcgaccact ggcctgcctg tttattctgg
     8881 ttggcgcggc tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga
     8941 ttgccaatat tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt
     9001 tattaacgct gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca
     9061 aacctgccac ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg
     9121 tactgctgat tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca
     9181 ataacaaaga gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat
     9241 ggtactccca tcagcgactg gcaaatctac cgctggtgcg aattggtgaa gacgcccacc
     9301 gcaacccgtt aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa
     9361 cctcgcgggc ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg
     9421 cgaaagataa cgtggtctat ttccctaata ccgcatcttg cggcacggca acggcagttt
     9481 cagtaccgtg catgttctcg gatatgccgc gtgagcatta caaagaagag ctggcacagc
     9541 accaggaagg cgtgctggat atcattcagc aagcgggcat caacgtgctg tggaatgaca
     9601 acgatggcgg ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga
     9661 atctgcctgg tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg
     9721 aagagtacat caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc
     9781 acggtccgac ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg
     9841 acaccaatga gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc
     9901 tggtttacgt cgactatatt gttgataaag cgattaatct gctgaaagaa catcaggata
     9961 aatttaccac cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca
    10021 tctatctgca cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccgatgc
    10081 tgctgtggct gtcggaggat tatcaaaaac ggtatcaggt tgaccagaac tgcctgcaaa
    10141 aacaggcgca aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa
    10201 ctggcgttga gacgaagtat taccaggctg cggatgatgt tctgcaaact tgcaggagag
    10261 tgagtgaatg aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct
    10321 ggcggcgcaa accgaaggct acacgtgcga tggcgtaaca accgcgcgga tggcggaaca
    10381 aagccttgag gccggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga
    10441 tggactgcat tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct
    10501 caccgctcgc gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta
    10561 tctggtgaag ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg
    10621 ccataataat cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg
    10681 ccgtcaggta tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct
    10741 gtcacggtta atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat
    10801 ctataactgg gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg
    10861 cgacaaagtg ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc
    10921 gaatgaggaa aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca
    10981 acggctgata ttgaccatcg gggccatttt gttggtgttt gagctgatca gcatcttctg
    11041 gctatggcat gaaagtaccg agcagattca gctgtttgag caggcgctac gcgacaatcg
    11101 caacaacgat cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc
    11161 cggcgtcttt atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac
    11221 ccgcccgctg gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc
    11281 cattgccatt cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct
    11341 ggtcagtcgc ctgacgagca cgctggataa cgaaaggtta tttaccgctg acgtcgcgca
    11401 cgaactgcga acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca
    11461 tcacattgat gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca
    11521 gctgctgcaa ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa
    11581 actgctggaa gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg
    11641 acagcaaacc ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac
    11701 cctgctgcgg atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccacaagg
    11761 cagcaacatt atgattaagc tgcaagaaga tggcggagcg gtcatggccg ttgaagatga
    11821 aggaccgggt attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga
    11881 cagccgttat ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca
    11941 tcacgggcag tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg
    12001 gctgaagaaa gatcagtacg tagcaaacca gatataaaga aagctgctga ccaccagcac
    12061 actgaacacg gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa
    12121 cctggaggag agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat
    12181 aagacagcgt cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg
    12241 caccaggcgg gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat catcgatttt
    12301 ctgctcgata ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc
    12361 cgccggtgtc gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat
    12421 taaaccaatc accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct
    12481 ttcgaccagc caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa
    12541 tgccgccgcc agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc
    12601 cataacgccc gtaaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag
    12661 gccgatgacg ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagggcaac
    12721 actaccaagt agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg
    12781 ctggacaaac agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga
    12841 gtaatgcagg ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac
    12901 gttggtggca attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat
    12961 ctctttaaac gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt
    13021 atcaacatgc tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat
    13081 aatccctaac ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc
    13141 ttcgccgaca atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat
    13201 agaaccgaag tccagccagc tgcccataaa gccacgttta cggtcagggg agtattccgc
    13261 aacaaatatc gacgccccgg tatattcacc gccgaccgag aaaccttgtg ccatcttaca
    13321 gatcaacagc agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat
    13381 acagaacgta ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata
    13441 tttatcgccc aacataccaa agaagagccc gccaagcggt cgaatcagaa agggaacgga
    13501 gaaagtggca agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt
    13561 acctaatgcg taagcaacaa aaccataaac accaaaatcg aaccattcca ttgcattacc
    13621 cagtgatgct gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg
    13681 aagggtaatc ggttttactt ttttcctttt cagcatagct ttcctcgcag agttggccca
    13741 tttccgcaaa cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg
    13801 atgcaatctc tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca
    13861 atcaaaccag ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta
    13921 aatttgtgat caacttcaca catggatttc gtttaggact cattgatgta actgattatt
    13981 tacgccaaat ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa
    14041 cagagtcgtg gcatccccag cgcgactgtt tgccagcgtt gggtcactga ccagcaaacg
    14101 ggctgtttat ctgccgtgga ccactttccc agccaactta aaaaatcagc atgctcccct
    14161 cccgaagagg caaaccactg atggtaatag tgacgcgtgg tcggtgccgc attgatactc
    14221 tctgccagat gttgtgtcgc tttctcgcgc aaatcaccca gtaacaacac caccgcttgc
    14281 gggcttccca gacggcaggc atcgccaaat gcaccccagc gcaactcctc cagaacgata
    14341 aaacagcgcc caggcaacga ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt
    14401 tgctcgctct gatcacctgc ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc
    14461 cagcgacaac gggctgaaaa ccactcatac aaggcggcaa tttgctgggg gagctccggg
    14521 aaagcagagt gctggcggtg atccattttt tgcagcagcg ccagatgctg cgcgatctgc
    14581 gtcagcgcca gatgaccggg atccagcatc gacgccagtt tttccaccat tgtcagtcgc
    14641 aggctaagcg gcgcatttaa catgccgccg ttcgtccatg tctgaagctg cacacgcgaa
    14701 agaagttcct gcatcagtcg ttcacgaaac tgctgctgat gggcttgtgg aaggcgggca
    14761 tcatcgccct gcgccagatc cactaaaaag cggggataaa ccgactccag cacgcgaccg
    14821 gggccgtcca gtaacgtctt ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat
    14881 cgcgtaacag ctgggcgtcg gtgtaaatcc agccgtgagt catcacagtc tgctgcaatt
    14941 gctgctgcat cagcctgacc accgattcat tttgttgacg cagagccagg ctttcgcgta
    15001 aacgcgtctg taattccgtc aaacatgaag cgaactcagc gcaaaaagta ttcatgcctg
    15061 ccgtaacaga ttcatcgacc tgctctgcca gaactttagc catttgttgg caataaagat
    15121 cgacttctgc gcttaaagct cgttgcaaca cactgtaatc aaccgtctct gtcggggatt
    15181 tctcatttcc ccgtccccag tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa
    15241 cgcctaaacg cgtgctctgc tcgtccgttg catcctggcg cgaaatgact tcactgaaca
    15301 gctggcgagt gttgacgtgg ggaactactc cgtgaaaaac aggaaaatga accccaggac
    15361 gaaaccctga ctcgctcaat tccattttga cttgttgctc aatgggccga ataacatcgg
    15421 ttaacacccg gcaaagggtg gattcaagct cggcaaaacg cagcgtaaag tcgcgactga
    15481 tggtgttctg cgccgtctgt aacagtgtct cacagcaggt acgaatctcg cttaacggct
    15541 ccgaatcatc ctgaaacaag gcggctaact gcgcattcag cgcatcttgc tgttgacgca
    15601 gaaagtggtt ggcggaggtc agggccagct cgatttcatg tttaatctcg ccgctcacct
    15661 gcgcctgatt gagttgcaat agctgcaaac tttcttcgac ctgatggata ttttgccgca
    15721 attgttcaca agcgacgttt aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct
    15781 gcgcgtaatt caacagttta tgcgcagcag atcgcagagc atacaacgag gcgttagcgt
    15841 aagcggcatg aagcaacgcc tgaattggct gggcgaacag cgaatcttcc cacaactgat
    15901 cggcagcatg acgaatatgt tcgaggtccg ccagatcggc atgacgccag cgcctgccga
    15961 gcgcggcatg ggcaaaatct tccacccagc gttgttgctc tggcgctggt aacttaccgt
    16021 tgttggctaa ctcatggcgc gctcgatttg ccaagtagcc ccacatcgac gacaccggaa
    16081 atatctgctg tggcgtaata cagcctttca tcagcgtccc ggaaatcagt gcccgcacct
    16141 ggtcggcgtc gtcactgtta cgatcctgtt gatcgaactt attgaccagc acatacagtg
    16201 gcaccgattg ccccaccgcc aaaatcgcct cacggacctc ttcatcggag atcgatttca
    16261 gttgcgtata atccagcacc gccagtaccg ccgaggcgcg tgccagctgc tggttaagca
    16321 ttttttgcag atgcggttgc ccggcttcat ttggcccggg ggtatccagt aacgtcaact
    16381 gaccgggata actctccagc cccgccagat ggacaaactc cacttcaatc acgggaatat
    16441 gctcaatggc ggcgtaagca gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca
    16501 ctaaatcatt caaacttttc agacaatgaa aaataggctg ggcacccaga taatattttt
    16561 cgaaagcgac gccattttcg atccgctgca taagcgcacg catatcttta tctatttcca
    16621 gcacatcggt cagatgctta atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta
    16681 aacaatcgat tggcgcgaca tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat
    16741 ggcgaataag cgtcggcagc gcagtcattg ggcgattacg attaggcaga acctccgtac
    16801 caacaatggc attaatggtg gttgatttcc ctgctttcat agtaccgaca atcgccagca
    16861 ccatttccag tcgggaaatt ttactcaact cattattcag catcgcgtga cgttcggcga
    16921 tattaggctg actccagggt aaagccagtt gtggcgcgtc gtctccgggt acagagagag
    16981 gcattttttc cagtaactgc aactgttggc gagaaagctg taacaggcgt tcagcctcct
    17041 gacttaactc atacagggtc tgtgtgtaca tagaaaattc ttccttaaag caaattttgt
    17101 tacttttatt tagccagatt gtttttgagt tctgtttgag ttctgttttt ggcttttata
    17161 attactgcaa gaaataattt tatatttagt gtgttgtttt ttatcagaat aaataacgtc
    17221 ttctgatgcg tttaaaacgt cagaaagata aaaatatcat gtgaataaaa aaaagaacaa
    17281 gtagagcatt aacattatct taaataataa atagaggcaa aaagattatt ttctttttgc
    17341 gtttcctttc aaatgaaaac gatcgtcgtc taaaatcagc agtacccccg acaaactcag
    17401 ggattttgtg tataattgcg gcctttttcg gcaatctgcc gttttttggc gcttttgccc
    17461 tgctgacttt tgaggaaatc cacatgtcat taccacactg cccaaaatgc aactccgaat
    17521 acacttacga agataacggc atgtacatct gcccggaatg tgcctacgaa tggaacgacg
    17581 cagaacctgc acaggaaagc gacgagctga tcgttaaaga tgctaacggc aatctgctgg
    17641 ctgacggcga cagcgttacc atcattaaag atctgaaggt gaaaggtagc tcttcgatgc
    17701 tgaaaattgg caccaaagtg aaaaacatcc gcctggttga aggcgaccat aacatcgatt
    17761 gcaaaatcga cggttttggt ccgatgaaac tgaaatctga gtttgtgaaa aagaactgat
    17821 tgtattgtga tcggtaagcc ggataaggcg ctcgcgccgc atccggcaac gatgccgact
    17881 gcctgatgcg acgcttgcgc gtcttatcag gcagcctctc atctgtataa atttcgaact
    17941 acacttaatt ggcttctctt aactgaggtc accatcatgc cgttaagtcc ctacctctct
    18001 tttgccggta actgtgccga cgcaattgct tattatcaac gtacgttggg cgctgaactg
    18061 ctctataaaa tcagcttcgg cgaaatgcca aaatcagcgc aggacagcgc cgagaactgc
    18121 ccttccggaa tgcaatttcc cgataccgcc atcgcccacg ccaatgtgcg cattgccgga
    18181 agcgacatca tgatgagcga tgccatgccg tcaggaaaag ccagctactc cggctttacg
    18241 ctggtgctcg attcgcaaca ggtcgaagaa ggaaaacgct ggtttgacaa tcttgccgct
    18301 aacggaaaaa tcgaaatggc ctggcaggaa actttctagg cgcatggctt tggcaaagtc
    18361 accgataaat ttggcgtacc gtggatgatt aatgtcgtca aacaacaacc aacgcaataa
    18421 cccgccggga ggcccgccct cccgcactgt catcgaattc ccgttaactc ttcatctgtt
    18481 agtcactttt aattaaccaa atcgtcacaa taatccgcca cgatggagcc acttttttag
    18541 ggaggctgca tcatgcaaac gattatccgt gtcgagaagc tcgccaaaac cttcaatcag
    18601 catcaggcgc tgcatgcggt tgctctgaac attcatcacg gtgaaatggt ggctctgctt
    18661 gggccgtcgg gttccggcaa atccaccctt ttacgtcact taagcggttt gattaccggc
    18721 gataaatccg ccggcagcca tatcgagctg ctgggccgca cagtccagcg cgaaggccgt
    18781 ctggcgcgcg atatccgcaa aagccgcgcc aacaccggct acatcttcca acaattcaac
    18841 ctggtgaacc gcctgagcgt actggagaac gtgctgattg gcgcgctcgg cagcacgccg
    18901 ttctggcgca cctgttttag ctggtttacc cgcgagcaga aacaacgcgc gttacaggcg
    18961 ctgacccgcg ttggcatggt gcattttgcc catcaacgcg tttccaccct ctccggcgga
    19021 cagcagcagc gtgtggcgat tgcccgcgcg ctgatgcagc aggcgaaggt gattctggcc
    19081 gatgaaccca tcgcctcgct ggacccggaa tccgcccgca tcgtgatgga caccctgcgc
    19141 gacatcaatc agaacgacgg catcaccgtg gtcgtcacgc tgcatcaggt ggattacgcc
    19201 ctgcgctact gcgaacgcat cgtcgccctg cgccaggggc acgttttcta cgacggcagc
    19261 agccaacagt ttgataacga acgttttgac catctctacc gcagcattaa tcgcatcgaa
    19321 gagaacgcga aagctgcctg acatccccat cattgaggaa aacgaatgaa cgctaagata
    19381 attgcctcgc tggccttcac cagcatgttc agcctcagca ccctgttaag cccggcacac
    19441 gccgaagagc aggaaaaggc gctgaatttc ggcattattt caacggaatc acagcaaaac
    19501 ctgaaaccgc aatggacgcc attcttacag gatatggaga agaagctggg cgtgaaggtg
    19561 aacgccttct ttgccccaga ctacgcaggc attatccagg gaatgcgctt caataaagtg
    19621 gatatcgcct ggtacggcaa cctgtcggca atggaagcgg tggatcgcgc caacggccag
    19681 gtcttcgccc agacggtcgc ggcggatgga tcgccaggtt actggagcgt gttgatcgtc
    19741 aacaaagata gtccgatcaa caacctgaac gatctgctgg cgaagcggaa agatctcacc
    19801 ttcggcaatg gcgatcctaa ctccacctct ggcttcctcg tccccggtta ctacgtcttt
    19861 gccaaaaaca atatctccgc cagcgacttt aagcgcaccg tcaacgccgg gcatgaaacc
    19921 aacgcgctgg ccgtcgccaa caagcaggtg gatgttgcca ccaacaacac cgaaaacctc
    19981 gacaagctga aaacctccgc gccagagaag ctgaaagaac tgaaggtgat ctggaagtcg
    20041 ccgctgatcc caggcgatcc gatcgtctgg cgcaagaatc tctccgaaac caccaaagac
    20101 aagatctacg acttctttat gaactacgga aaaacgccgg aagagaaagc ggtgctggaa
    20161 cgcctgggct gggcaccgtt tcgcgcctcc agcgacctgc aactggtgcc gattcgccag
    20221 ctcgcactgt ttaaagagat tcagggcgta aaaagcaata acggactgaa tgagcaggac
    20281 aagctggcga aaaccaccga gattcaggcg cagctggatg acctggaccg cctgaacaac
    20341 gcgttaagcg cgatgagttc ggtgagtaaa gcggtgcagt aatgtgctcg atcggtcccc
    20401 tctcccttga gggagagggt tagggtgagg gggaaatgcg cacgctgatg ccctcacccc
    20461 ggccctctcc cacagggaga gggagaaaac attaaacgga gccaaacatg caaaccatca
    20521 ccatcgcccc acccaagcgc agctggttct cgcttctgag ctgggccatt gttctcgccg
    20581 tgttggtcgt ctcgtggcag ggcgcggaaa tggccccgct tacgctgatc aaagacggcg
    20641 gcaacatggc gaccttcgcc gccgacttct tcccgcccga tttcagccag tggcaggatt
    20701 acctcaccga aatggccgtc acgctgcaaa tcgccgtctg gggcaccgcg ctggcagtgg
    20761 ttctctccat ccccttcggc ctgatgagcg ccgaaaacct ggtgccgtgg tgggtttacc
    20821 agcccgttcg ccgcctgatg gacgcctgcc gcgccattaa cgaaatggtc ttcgccatgc
    20881 tgttcgtggt cgccgtcggt ctcggaccgt tcgctggcgt gctggcgcta tttattcaca
    20941 ccaccggcgt gctctcgaaa ctgctttccg aagcggtgga agcgattgaa cccggcccgg
    21001 tggaaggcat tcgcgccacc ggcgccaaca agctggaaga gatcctttac ggcgtgctgc
    21061 cacaggtaat gccgctgctg atctcctact ccctctatcg cttcgaatcc aacgtccgct
    21121 cggcgaccgt cgtcggcatg gtcggtgcag gcgggatcgg cgtcaccctg tgggaagcga
    21181 ttcgcggttt ccagttccaa caaacctgcg ccctgatggt gcttatcatc gtcacggtca
    21241 gcctgctgga tttcctctct caacggttgc gtaagcactt tatctgataa gtgaggcatt
    21301 gatatctatg cacttgtcta cacatccgac cagctaccca acacgctatc aggagatagc
    21361 cgcaaaactt gagcaggagc ttcgtcaaca ctatcgctgc ggcgactatc ttcccgccga
    21421 gcagcaactg gcggcgcgct ttgaggtaaa ccgccacacc ctgcgccgcg ccatcgacca
    21481 actggtggaa aaaggctggg tacagcgccg tcagggcatc ggcgtgctgg tgctgatgcg
    21541 cccgttcgat tacccgctca acgcccaggc gcgttttagc cagaacctgc tggaccaggg
    21601 cagccatccc accagcgaaa aactgctgtc ggtattgcgc cccgcgtccg gtcacgttgc
    21661 tgacgcgctg gggattaccg agggtgagaa cgttatccat ttgcgcaccc tgcgtcgcgt
    21721 caacggcgtc gcgctctgtt taatcgacca ctactttgcg gacctcaccc tctggccgac
    21781 actgcaacgt ttcgacagcg gttcgctgca cgattttctg cgcgagcaaa ccggaattgc
    21841 gctgcgccgc agccagacgc ggatcagcgc ccgccgcgcc caggccaaag agtgccagcg
    21901 ccttgaaatc ccgaatatgt cgccgctgct gtgcgtgcgc acccttaacc accgtgacgg
    21961 tgaaagcagc ccggcggagt actccgtcag cctgacgcgc gccgacatga ttgaattcac
    22021 tatggagcac tgaatgcacg cagataccgc gacccgccag cactggatgt ccgtactggc
    22081 gcacagccaa ccggctgaac tggcagcccg cctgaacgcg ctgaacatca ccgccgacta
    22141 tgaagtgatc cgcactgccg aaactggcct ggtgcagatt caggcgcgaa tgggcggtac
    22201 cggcgaacgc ttttttgccg gcgacgccac gctgacccgt gccgccgtac gcctgactga
    22261 cggcaccctc ggctacagct gggtgctggg gcgtgataaa cagcacgccg aacgctgcgc
    22321 gctgattgat gcgctgatgc agcaatctcg ccactttcaa aacttatcag aaacccttat
    22381 tgccccgctg gacgctgacc gtatggcacg cattgccgca cgccaggccg aagtgaacgc
    22441 cagccgggtc gacttcttta cgatggttcg cggagacaac gcatgaccct ggaaaccgct
    22501 tttatgcttc ccgtgcagga tgcccagcac agttttcgtc gcctgttaaa ggccatgagc
    22561 gagccgggcg tgattgtcgc cctgtatcag ctcaaacgcg gctggcagcc gctgaatatc
    22621 gccaccacca gcgtgctgct gacgctggcc gataacgaca cgccggtgtg gcttgccacg
    22681 ccattaagta acgatatcgt caatcagagc ctgcgttttc ataccaacgc gccgctggtc
    22741 agccagccgg aacaggcgac cttcgcggtg acggatgagg cgatttccag cgaacagctc
    22801 aacgcccttt ccaccgacac cgccgttgcg ccggaagcgg gcgcgacgct gattttacag
    22861 gtcgccagcc tgagcggcgg acgcatgttg cgtctcaccg gcgcgggcat tgccgaagaa
    22921 cgaatgatcg ctccgcagct gccagagtgc attctgcacg aactcaccga gcgcccgcac
    22981 ccgttcccgc tcggcatcga cctaatcctg acctgcggcg agcgcctgct ggctatcccg
    23041 cgaaccactc atgtggaggt gtgctgatgt acgttgccgt gaaagggggc gaaaaggcga
    23101 tcgacgccgc ccacgccctg caagagagcc gacgccgggg cgataccgat ttgcccgaac
    23161 tgagcgtcgc ccagattgaa cagcagctta acctcgcggt ggatcgcgtg atgacagaag
    23221 gcggcattgc cgaccgcgaa ctggcggcgc tggcgctgaa acaggccagc ggcgataacg
    23281 tcgaagcgat tttcctgcta cgcgcctacc gcaccacgct gacgaaactg gcggtaagcg
    23341 agccgctcga caccacggag atgcgtctcg aacggcgtat ttcagcggtt tataaagata
    23401 ttcccggcgg ccagctgctt ggcccaacct acgactacac ccatcgcctg ctcgatttca
    23461 ctctgctggc aaacggcgaa gcgccgacgc tgaccaccgc cgacagcgaa caacagccgt
    23521 cgccgcacgt tttcagcctg ctggcgcgtc aggggctggc gaagtttgaa gaggatagcg
    23581 gagcacagcc ggatgacatc acccgcacgc cgccggttta cccctgttca cgttcttccc
    23641 gtttgcagca gttaatgcgc ggcgacgaag gctatttgct ggcgctggct tactccaccc
    23701 agcgcggtta cgggcgcaac cacccgttcg cgggcgagat ccgcagtggt tacatcgacg
    23761 tgtcgattgt gccggaagag ctgggatttg cggtaaacgt cggcgaactg ctgatgaccg
    23821 agtgtgaaat ggtcaacggt tttatcgacc cgccggatga gccgccgcac ttcacgcgcg
    23881 gctacgggct ggtattcggc atgagcgagc gcaaagcgat ggcaatggcg ctggttgacc
    23941 gtgcgttgca agccccggaa tacggcgagc acgcgacagg cccggcgcag gatgaagagt
    24001 tcgtgctggc gcatgccgac aacgtcgaag ccgcaggctt tgtctcgcac ctcaaactcc
    24061 cgcactacgt cgatttccag gccgaactgg agctactcaa acgtctgcaa caggagcaga
    24121 accatggcta atctgagcgg ctacaacttt gcctacctcg acgagcagac caaacgcatg
    24181 atccgccgcg ccatcttaaa agcggtggcg atccccggtt atcaggtacc gtttggcggg
    24241 cgcgagatgc cgatgccgta cggctgggga actggtggta tccagctcac cgccagcgtg
    24301 attggcgaaa gcgacgtgtt gaaggtgatt gaccagggtg cggatgacac caccaacgcc
    24361 gtgtcgattc gcaacttctt taagcgcgtg accggggtaa acaccactga atgtacggac
    24421 gatgcgacgc ttatccagac gcgtcaccgc atccccgaaa cgccgctgac cgaagatcag
    24481 atcattatct tccaggtgcc aatcccggaa ccgctgcgct ttatcgagcc gcgcgaaacg
    24541 gagacccgca ctatgcacgc gctggaggag tacggcgtga tgcaggtgaa actgtatgaa
    24601 gatatcgccc gcttcggtca tatcgccacc acctatgctt atccggtgaa ggtgaacggg
    24661 cgctacgtga tggacccgtc gccgatcccg aaattcgata acccgaaaat ggacatgatg
    24721 cccgccctgc agctgttcgg cgcagggcgc gagaagcgca tctatgcggt gccgccgttt
    24781 acccgcgtgg aaagtctcga tttcgacgat cacccgttca ccgttcagca gtgggatgag
    24841 ccatgcgcca tctgcggatc gacccacagc tatcttgatg aagtggtgct ggatgacgcc
    24901 ggaaaccgca tgtttgtctg ctccgatacc gattattgcc gccaacagag cgaggcgaaa
    24961 agccaatgaa tcaaccgtta ctttcggtca ataacctgac ccacctttac gcgccgggca
    25021 aaggctttag cgatgtctct tttgatttat ggccggggga agtgctgggc attgtcgggg
    25081 aatccggctc cgggaagacc acgctgctga agtcgatctc cgcgcgtctg acgccgcagc
    25141 agggggagat tcactatgaa aaccgttccc tctatgcaat gagcgaggcc gaccgccgtc
    25201 gcctgctgcg caccgaatgg ggcgtggtgc atcagcatcc gctcgacggc ctgcgccgcc
    25261 aggtgtcggc aggcggcaat atcggcgagc ggctgatggc gaccggggcg cgacattacg
    25321 gcgatattcg cgctaccgcg cagaagtggc tggaagaggt ggagattccc gccaaccgta
    25381 tcgacgacct gccgaccacc ttttccggcg gtatgcagca gcgtttgcag attgcccgca
    25441 acctggtgac gcatccgaag ctggtgttta tggatgaacc gaccggcggg ctggatgtgt
    25501 cggtgcaggc ccgcctgctc gacctgctgc gcggactggt ggtggagctg aacctcgcgg
    25561 tagtgattgt cacccacgat ttaggcgtcg cccgcctgct ggcggaccgt ttgctggtga
    25621 tgaagcaggg gcaagtggtg gagagtgggt taaccgaccg cgtgctcgac gacccgcatc
    25681 atccgtatac acagctgctg gtgtcatcgg ttttgcagaa ttgagccggt gccggatgcg
    25741 gcgtaaacgc cttatccggc ctacaaatgc gctccccgta ggtcggataa gacgcgtcag
    25801 cgtcgcatcc gacacccgaa ccacgaggcg aaaaatgatt aacgtacaaa acgtcagtaa
    25861 aaccttcatc ctgcaccagc aaaacggcgt gcgcctgccc gtcctcaacc gcgcctcgct
    25921 catcgtcaac gcgggcgaat gcgtggtgct ccacggccat tccggcagcg gaaaatcaac
    25981 cctgctacgc tcgctgtacg ccaactatct gcccgacgaa gggcaaatcc agatcaaaca
    26041 cggtgacgaa tgggtcgatc tggtcaccgc gccagcgcgc aaagtggtgg agatccgcaa
    26101 aaccaccgtt ggctgggtga gccagtttct gcgcgtcatc ccgcgtattt cggcgctgga
    26161 agtggtgatg cagccgctac tcgataccgg cgtaccgcgt gaagcctgcg ccgctaaagc
    26221 cgcgcgtctg ctcacccgcc tgaacgtgcc ggaacgcctg tggcatctgg cgccatcgac
    26281 cttctccggc ggcgaacagc agcgcgtcaa catcgcccgc ggctttatcg tcgactaccc
    26341 cattctgctg ctcgacgaac ctaccgcctc gctggacgcc aaaaacagcg ccgccgtagt
    26401 cgagctgatt cacgaagcca aagcccgtgg cgcggccatc gtcggcatct tccacgacga
    26461 agccgtacgt aatgacgttg ccgaccgcct gcacccaatg gaagcctctt catgattatc
    26521 aataacgtca aattggtgct ggaaaacgaa gtggtacacg gttcactgga ggtgcaggac
    26581 ggcgaaatcc gcgcctttgc cgaaagccag agccgcctgc cggaggcgat ggacggcgaa
    26641 ggcggctggc tgctgccggg gctgattgag ctgcataccg ataatctgga taaattcttc
    26701 accccgcgcc cgaaagttga ctggcctgcc cactcggcga tgagcagcca cgacgcgctg
    26761 atggtggcga gcggcatcac caccgtactg gatgccgtgg caattggcga cgtgcgcgac
    26821 ggcggcgatc ggctggtgaa tctggagaag atgatcaacg ccatcgaaga gacgcagaaa
    26881 cgcggcgtca accgcgccga gcaccgtctg catctgcgct gcgaactgcc gcatcacacc
    26941 acgctgccgc tgtttgaaaa actggtgcag cgcgagccgg tgacgctggt gtcgctgatg
    27001 gaccactcgc cgggccagcg ccagttcgcc aatcgcgaga agtatcgcga atattatcag
    27061 ggcaaatact ccctcactga tgcgcagatg cagcagtacg aagaagagca actggcgctc
    27121 gccgcacgct ggtcgcagcc gaatcgcgaa tccatcgccg ccctgtgccg cgcgcgaaaa
    27181 attgcgcttg ccagccacga tgacgccacc cacgcccacg ttgctgaatc tcaccagctt
    27241 ggcagcgtga tcgccgaatt tcccaccacg ttcgaagcgg cggaagcctc gcgcaagcat
    27301 ggcatgaacg tgctgatggg cgcgccgaat attgtgcgcg gcggctcgca ctccggcaac
    27361 gtggcggcca gtgaactggc gcagcttggc ctgctggata tcctctcttc cgactactac
    27421 cccgccagcc tgctcgatgc ggcatttcgc gtcgccgatg acgagagcaa ccgctttacg
    27481 ctgccgcagg cggtgaagct ggtgactaaa aatccagcgc aggcgcttaa tctccaggat
    27541 cgcggggtga ttggcgaggg caaacgcgcg gacttggtgc tggcgcatcg caagggcaat
    27601 cacattcata tcgaccacgt ctggcgtcag ggtaaaaggg tgttctgatg atgggaaaac
    27661 tgatttggtt aatggggccg tccggctccg ggaaagatag cctgctggcg gaactccgcc
    27721 tgcgggaaca aactcagcta ctggtggcgc atcgctacat cacgcgcgac gccagcgccg
    27781 gaagcgaaaa ccatatcgct ctgagcgagc aggagttttt tacccgcgcg gggcagaacc
    27841 tactggcctt aagctggcac gctaacggtc tgtattatgg cgtcggcgtc gagatcgacc
    27901 tctggctgca cgccggattc gacgtgctgg tcaacggctc acgcgcccat ctgccgcagg
    27961 cgcgggcgcg ctatcaatcg gcgctgctgc ccgtctgttt acaggtttcg ccggagatcc
    28021 tccgccagcg cctggaaaac cgtggccgtg aaaacgccag tgaaattaac gcccgcctgg
    28081 cgcgcgccgc ccgctatact ccacaggatt gccatacgct caacaatgac ggcagcctgc
    28141 gccagtcggt cgacacgctg ctgacgctga ttcatcagaa ggagaaacac catgcctgct
    28201 tgtgagcttc gcccggccac gcagtatgat accgacgcgg tttacgcgct gatttgtgag
    28261 ctaaaacagg cggagtttga ccaccacgca tttcgcgtgg gctttagcgc caatctgcgc
    28321 gacccgaaca tgcgctatca tctggcgctg ctggatggcg aggttgtcgg catgatcggc
    28381 ctgcatttgc agtttcatct gcatcatgtc aactggatcg gcgaaattca ggaactggta
    28441 gtgatgccgc aggcgcgcgg cctgaacgtc ggtagcaagt tgctggcgtg ggcagaagaa
    28501 gaagcccgcc aggccggggc cgaaatgacc gagctttcga ccaacgtgaa gcgccacgac
    28561 gcgcaccgtt tctatctgcg agaaggctac gagcagagtc acttccgctt caccaaggcg
    28621 ctgtaacatg agtctgaccc tcacgctcac cggcaccggc ggcgcacagg gcgttccggc
    28681 atggggctgc gagtgcgcgg cctgcgaccg agcgcggcgc tcgccgcagt atcgccgcca
    28741 accgtgcagc ggcgtagtga agtttaacga ggcgatcacc ctgatcgacg ccgggctgca
    28801 cgatctcacc gatcgctggt ctcccggatc gttccagcag tttttgctga cgcattatca
    28861 tatggatcac gtccaggggc tgtttccgct gcgctggggc gtgggcgatc cgatcccgat
    28921 ctacggcccg ccggatgaac agggctgcga cgatctgttt aaacatcccg ggctgcttga
    28981 tttcagccac acggtggaac cgtttgtggt gtttgatttg caggggttgc aggtcacgcc
    29041 gctgccgctt aaccactcaa aactcacttt cggctattta ctggaaacgg cgcacagccg
    29101 ggtggcgtgg ctgtctgaca ccgctggctt gccggaaaaa acgctgaaat ttttacgcaa
    29161 taatcagccg caggtaatgg tgatggattg cagtcacccg ccgcgcgcgg atgcaccgcg
    29221 taatcactgt gatttaaata ccgtgcttgc gctgaatcag gttatccgct cgccacgggt
    29281 gattctgacc catatcagcc accagtttga tgcgtggctg atggaaaacg cactaccgtc
    29341 agggtttgag gtggggtttg atgggatgga gattggggtg gcgtgatgag agggaatgtg
    29401 cgcgctggcc ccctcaccct aaccctctcc ccagaggggc gaggggaccg attgtgctca
    29461 atattgaata ttgcgctcgt tttctccctc tccccattgg ggtgaggggc gatgcctgct
    29521 ccatacccaa cctcatcgcc catactcatc ttccattctc cgctcttcat cctccagttg
    29581 ccgacgctcc tgatcaagct ggcgctggcg atcgtccagc tgcctgcggc gatcttcaaa
    29641 ctggcggcgg cggtcgtcat attgtctgcg ccgatcgtcg ctcacttcac gctgccagcc
    29701 gtcgtcgcgc gaatcttcat agtctcgccc acggtcaggg ttataagcgt cattaatcgc
    29761 ctgctgaata ttaccaatag tatcgtcgat aatatcagcc tgggccggaa cggtggacag
    29821 cgtgagcagg gtgaataaaa gaaatagcgg aaagcgtttc attagccaac ctcaaaaaga
    29881 aactctatcc acattagtca ttactcatcc atgcaagtag tggatgaatc tcaatttctc
    29941 cgctgctcta ttgccgtaat cgcctccacg cgttgttgat gacgaccgcc ttcgtactgt
    30001 gcgcccagcc acgcatccac aatcattttt gccagttcga ggccaaccac tcgtgaacca
    30061 aaagccagtg caacttcact ggcataatgc ggataatcag tgcgctctga cgacctggtg
    30121 tctttatcaa tcacctcact cattgaccat gaataaaagg ctggcacagc aacgcagaat
    30181 cctgcggtgc cagcgacatg accgcccatt gctccggtac gttttttaaa aagaaacacc
    30241 ggttgcattg ataaattgtg ctcagtattt gccgaagtac aatatatata ataaatatat
    30301 aaaacacctc atgttattta cagagataat aaattcgcgt aattttccct cttcagcaca
    30361 aatgcctaat aaaatggatc gttcagctca cacttccact tattaaagtc gcaaattccc
    30421 aattatgata accccagctt taaatgacgt attttgcatg gattacatat ttacgacata
    30481 aatgataaat ggagatttac atgaataact ctcggttatt ccgtttgagc agaattgtta
    30541 ttgcgttaac tgccgccagc ggcatgatgg taaataccgc taacgcgaaa gaggaagcga
    30601 aagccgccac tcaatatacc caacaggtta atcagaatta cgccaaatca ttaccgttta
    30661 gcgatcgtca ggattttgac gacgctcagc gtggatttat cgctccgctg ctggatgaag
    30721 gtattctgcg cgatgctaat ggcaaaccat attatcgcgg ggaagattat aaatttgata
    30781 tcaatgcgcc cgcgcccgaa accgttaacc ctagcctgtg gcgtcagtcg cagttaaacg
    30841 gtatttccgg cctgtttaaa gtcaccgaca gaatgtacca ggttcgcggt caggatatct
    30901 ccaacatcac ctttatcgaa ggtgataccg ggattattgt catcgacccg ctggtgacgc
    30961 caccgagcgc aaaagccgcc cttgaccttt acttccaaaa tcgcccgcaa aaaccgattg
    31021 ttgcggttat ttatacccac agccataccg accactatgg cggcgtgaaa ggcattatct
    31081 ctgaagctga tgtgaaatcc ggtaaagtgc aggttatcgc ccctgctggc tttatggacg
    31141 aagccatcag cgaaaacgta ctggcgggta atattatgag ccgccgtgca ctttactcct
    31201 acggcctgct gctggcgcat aaccctcagg gtaacatcgg caatggtctt ggcgtaacgc
    31261 tggcatcggg ctacccgagc atcatcgcac cgaacaaaac catcaccaaa accggtgaga
    31321 agatgattat cgacggcctg gagtttgact tcctgatgac cccaggtagc gaagcaccag
    31381 ccgaaatgca cttctatatt ccggccctga aagcgctgtg taccgccgag aacgccacgc
    31441 ataccctgca caacttctac actctgcgcg gcgcgaaaac ccgcgacacc agcaagtgga
    31501 ccgagtatct gaacgaaacg ctggatatgt ggggtaacga cgcggaagtc ctgtttatgc
    31561 cgcacacctg gccggtctgg ggcaataagc atatcaatga ttatattggt aaatatcgcg
    31621 atactatcaa gtacattcac gaccagaccc tgcacctggc gaaccagggc tacaccatga
    31681 atgaaatcgg cgacatgatt aaactgccgc ctgcacttgc caataactgg gccagccgtg
    31741 gctattacgg ttctgtcagc cacaacgccc gcgcggtgta taacttctat cttggctatt
    31801 acgacggtaa cccggctaac ctgcatccgt atggtcaggt ggagatgggt aaacgttacg
    31861 tgcaggcgct gggcggttct gcccgtgtca tcaacctggc gcaagaagcg aacaagcaag
    31921 gtgattaccg ctggtcggca gaactgctga aacaggtgat tgccgccaac ccgggtgacc
    31981 aggtcgcgaa gaatctgcaa gcgaataact ttgaacagct gggctatcag gccgagtccg
    32041 ccacctggcg cggtttctac ctgaccggcg cgaaagagct gcgcgaaggg gtgcataagt
    32101 tcagccacgg caccatcggt tccccggaca ccattcgcgg gatgtcggtc gaaatgctgt
    32161 tcgactttat gtccgttcgc ctcgatagcg cgaaagccgc gggtaaaaat atcagcctga
    32221 acttcaatat gagcaatggc gataacctca acctgacgct gaacgatagc gtgcttaact
    32281 accgtaaaac actgcaatcc caagctgacg cctctttcta catcagccgt gaagatctgc
    32341 acgccgtgct gaccgggcaa gccaaaatgg cggatctggt aaaagcgaag aaagccaaaa
    32401 ttattggcaa tggcgcgaaa ctggaagaaa ttatcgcctg tctggataat ttcgatttgt
    32461 gggtgaatat cgtaacccca aattaatgtc actcgctttt gggggcgctg aagcccccaa
    32521 aagcatttat tttaaaaata tatacagcca attaataaat aaaatatata agttatgaat
    32581 gcggtgactg aataaagcta tttttattca tcgagaaaat agccatactg acgccttcaa
    32641 tggatattga tattcactac agatattgtt cgcagcagac aggatgtatt taatatgcca
    32701 acagttctct ctcgcatggc gatgcaactc aaaaaaacag cctggataat tcccgtcttc
    32761 atggtttcgg gatgctcatt atctccggca atcccggtga tcggtgctta ttatcccagc
    32821 tggtttttct gcgccattgc cagccttatt ttgatgctca tcacgaggcg agttattcag
    32881 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
    32941 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
    33001 ccgaaaaaag ctcct
//
LOCUS       DAETQN010000037        32837 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.037, whole genome shotgun sequence.
ACCESSION   DAETQN010000037 DAETQN010000000
VERSION     DAETQN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.037"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            267..1925
                     /locus_tag="K0W68_001744"
     CDS             267..1925
                     /locus_tag="K0W68_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBI3073326.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="K0W68_001745"
     CDS             complement(1922..2878)
                     /locus_tag="K0W68_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBI3073327.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="K0W68_001746"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="K0W68_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBI3073328.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="K0W68_001747"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="K0W68_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBI3073329.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="K0W68_001748"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="K0W68_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBI3073330.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="K0W68_001749"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="K0W68_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBI3073331.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="K0W68_001750"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="K0W68_001750"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBI3073332.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="K0W68_001751"
     tRNA            complement(9740..9827)
                     /locus_tag="K0W68_001751"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            complement(10081..10299)
                     /gene="infA"
                     /locus_tag="K0W68_001752"
     CDS             complement(10081..10299)
                     /gene="infA"
                     /locus_tag="K0W68_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBI3073333.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10584..11288)
                     /gene="aat"
                     /locus_tag="K0W68_001753"
     CDS             complement(10584..11288)
                     /gene="aat"
                     /locus_tag="K0W68_001753"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBI3073334.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="K0W68_001754"
     CDS             complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="K0W68_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBI3073335.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="K0W68_001755"
     CDS             complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="K0W68_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBI3073336.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="K0W68_001756"
     CDS             complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="K0W68_001756"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBI3073337.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16451..16945
                     /gene="lrp"
                     /locus_tag="K0W68_001757"
     CDS             16451..16945
                     /gene="lrp"
                     /locus_tag="K0W68_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBI3073338.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17080..21147
                     /gene="ftsK"
                     /locus_tag="K0W68_001758"
     CDS             17080..21147
                     /gene="ftsK"
                     /locus_tag="K0W68_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBI3073339.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21302..21913
                     /gene="lolA"
                     /locus_tag="K0W68_001759"
     CDS             21302..21913
                     /gene="lolA"
                     /locus_tag="K0W68_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBI3073340.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21924..23267
                     /gene="rarA"
                     /locus_tag="K0W68_001760"
                     /gene_synonym="mgsA"
     CDS             21924..23267
                     /gene="rarA"
                     /locus_tag="K0W68_001760"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBI3073341.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23358..24650
                     /gene="serS"
                     /locus_tag="K0W68_001761"
     CDS             23358..24650
                     /gene="serS"
                     /locus_tag="K0W68_001761"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBI3073342.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24889..27333
                     /gene="dmsA"
                     /locus_tag="K0W68_001762"
     CDS             24889..27333
                     /gene="dmsA"
                     /locus_tag="K0W68_001762"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HBI3073343.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27344..27961
                     /gene="dmsB"
                     /locus_tag="K0W68_001763"
     CDS             27344..27961
                     /gene="dmsB"
                     /locus_tag="K0W68_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBI3073344.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27963..28826
                     /gene="dmsC"
                     /locus_tag="K0W68_001764"
     CDS             27963..28826
                     /gene="dmsC"
                     /locus_tag="K0W68_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HBI3073345.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28862..29488)
                     /locus_tag="K0W68_001765"
     CDS             complement(28862..29488)
                     /locus_tag="K0W68_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBI3073346.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29803..30951
                     /locus_tag="K0W68_001766"
     CDS             29803..30951
                     /locus_tag="K0W68_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3073347.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31161..32591
                     /locus_tag="K0W68_001767"
                     /pseudo
     CDS             31161..32591
                     /locus_tag="K0W68_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(32592..>32837)
                     /locus_tag="K0W68_001768"
     CDS             complement(32592..>32837)
                     /locus_tag="K0W68_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3073348.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         8057 a         8030 c         8813 g         7937 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct
     9841 accaaacctc atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag
     9901 aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc agtatatcta
     9961 catcgagaca agttacggac ttatacttcc aaagtacttc atacatatca caaaataaaa
    10021 aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa
    10081 tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt
    10141 cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc
    10201 agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag
    10261 aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact
    10321 accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg
    10381 ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca
    10441 aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat
    10501 tccttatcgg cagcggggta atgagcataa ccaactctgc gaccgcaatt ataacactct
    10561 ggggagaaat gtgccgaaaa cattcatttt tgtggtgaaa acaagcatcg tggcacccag
    10621 aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattcagata atcccgacgg
    10681 ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca gtcgataagc
    10741 ttaccgccat gactgataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt
    10801 tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac gccgtacata
    10861 ccgccgacaa gctcatcttc atgccagact tcaatggagt gggcatgacc gagttcgtga
    10921 aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg
    10981 gcacaacctt caatgacctg accaaaagcg taattcatcg tgacacgata gggcgagcgt
    11041 ttatgaaatc gcttcatact acggctgata tgcagtgatt caggccaaag caccgcgcga
    11101 ggatcgggcg accaccagag gatgggggcg cctggagaaa accacggaaa aataccacgc
    11161 tggtaagcca ttaacaggcg cgcagggcta agatcgcccc ccagagccag caggccgtta
    11221 ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc
    11281 aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct
    11341 gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa
    11401 taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat
    11461 gggtgaccat taacaccgtt ttctcacgca tcatttctgc aagcaattca aggatctggc
    11521 tttcggttgt ggcatctaag ccttcggtag gttcatccag caacaccagt ggcgcatcat
    11581 gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc tggcgtccgc
    11641 cttcacctaa ccaactgttg agacctgcgt cctcgagcag cttctccaga ccaacgcgac
    11701 gcaagatctc cgacagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac
    11761 gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag
    11821 ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc tgttgcgggt
    11881 cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa
    11941 tcgcaatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt
    12001 gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg gcaacacgag
    12061 tttgggtatc agggaaggtg acttccggtt tttgatccgt taaatcagtg atccgaacgg
    12121 cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa
    12181 acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc
    12241 caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca
    12301 ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc agccattgaa
    12361 tttctgtatt ctctagttgc gtgcgataac gatcgctggc accaaaaatg gtcagctcag
    12421 cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga tgagtcagat
    12481 tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa agcgtcagta
    12541 acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgactgtca
    12601 ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat
    12661 cgagcgtatc aacatccgcc accacgcgat tgagcaattc gccctgacga tagcgcgcca
    12721 gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga tgctgcaaca
    12781 cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca gtacgggtga
    12841 ttgcggcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcac
    12901 cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacaga ccgatactgg
    12961 cgagcagcgt cacaattgcc agcacaatac caagacttaa catccattta tgacgtttat
    13021 acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagta
    13081 atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc tcaataatct
    13141 gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga
    13201 ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg cgctgttcac
    13261 tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta
    13321 acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca gcctggtcgc
    13381 caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc caggcgttat
    13441 ccagcgctgc ttgtaattca tgttcgctgg catcaggtcg cgccagtagt acgttatccc
    13501 gtaatgttgc tgccggtaat tgtggatttt gcccaaccca ggagagatgt ttacgccatg
    13561 attctggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc tgatatgaga
    13621 gaaaaccaga aagcgcgttc aacagtgaac ttttacctga accgctgcga ccaaccaaca
    13681 cggcacgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt ttaccttccg
    13741 gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg atcggtcgat gctaattcca
    13801 cctcgccacg ctgcggatgg gcgagcgggg tttccataaa cgttttcaga ctgtcagctg
    13861 caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa
    13921 actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcgtagt
    13981 gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg
    14041 acagcgaggt aaaaaattcg agaatgccgg aggataaaaa tgccagccgt agcacttcca
    14101 ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac
    14161 gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc
    14221 gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca
    14281 taaacaacgg aattaacggt gcagtgccca gcagaatgag cgccgcagcc cagtttgagg
    14341 ggaagattgc caccacaatc agcaacggca ccgacactgc cagcgccatt tgcggcaggt
    14401 agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc
    14461 ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac
    14521 ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga
    14581 ccgcccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt
    14641 cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg
    14701 caatgatcaa tatgccgctt acaaagccca gcagacgaga aatattcagc caacgttggg
    14761 agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgagat ttattcattg
    14821 cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac ggggaaaaaa
    14881 taaaggcgac ccatagtcgc aaggtgtcgc cttctttact tttgttactg atttgtaaaa
    14941 ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc
    15001 agtaccggcc gaagtaatgg cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa
    15061 gacgccagga atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta
    15121 gccgttttcc agttccagct gcccttcgaa aatcgcagta ttcgggctgt gaccgatagc
    15181 aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag
    15241 acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtac ggttggtgtg
    15301 cagaatgatg ttgccgttct ccactttatc catcagacgc ttaataagga ttttttccgc
    15361 gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag acagatacag
    15421 cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa
    15481 gaaaccgtcg caggttgcac aagcagaaac cccacggcct ttaaaggctt cttcagaggg
    15541 caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc
    15601 gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt tgatatgatc
    15661 aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc atgcgctcca ttaataacgg
    15721 accggtcaga tcgtttggat caccaggcca gttttccact tccgtggtgg tggtcagttg
    15781 gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac
    15841 agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt
    15901 gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga
    15961 cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc
    16021 aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga
    16081 ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat
    16141 gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaaacagt
    16201 attcttatat gcgcataacc atgcatgtaa ataccatgtt taccgtgcta gtgaaatcta
    16261 cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc aacggtcttc
    16321 tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg
    16381 gtagcgactc tgaacagtga tgtttcaggg gcagacagga gtagggaagg aatacagaga
    16441 gacaataata atggtagata gcaagaagcg ccttggcaaa gatctcgacc gtatcgatcg
    16501 taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg
    16561 tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt
    16621 tattcagggc tatacggcgc tgcttaaccc ccattatctg gatgcatcac ttctggtatt
    16681 cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca ataccgctgt
    16741 acaaaaactt gaagaaattc aggagtgtca tttagtatct ggtgatttcg actacctgtt
    16801 gaaaacacgc gtgccggata tgtcagctta ccgtaagttg ctgggggaaa ccctgctgcg
    16861 tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca agcagagtaa
    16921 tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt attttgatta
    16981 cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt gtccgtttta
    17041 gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga atacactgaa
    17101 gacaaagaag tcacattgac aaagttaagt agcggccgcc gccttctgga ggcgttgctg
    17161 atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt taacccttcg
    17221 gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg tgggatgccc
    17281 ggtgcgtggt tggcagatac gctgttcttt atttttggcg tgatggctta caccattccc
    17341 gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagcga cgaatacatt
    17401 gattattttg ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct tacctcctgt
    17461 ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg cgtcattggc
    17521 agcttactaa gcactacgct acaaccactg ctacacagta gcgggggaac tattgcgctg
    17581 ctctgcgttt gggcagcggg cctgacgttg ttcaccggtt ggtcatgggt gaccattgct
    17641 gaaaaactcg gcggctggat tttaaacatt ctcaccttcg ccagtaatcg tacccgtcgc
    17701 gatgatacct gggtcgatga agatgagtat gaagacgacg aagagtatga agatgaaaat
    17761 cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt
    17821 aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagacgc tgcgttgttc
    17881 tccggtaagc ggatggatga tgacgaagag attacctaca ctgcacgcgg tgtggctgcc
    17941 gaccctgacg acgtcctatt ttcgggcaat cgtgcaacgc agccagaata tgacgaatac
    18001 gatccattat taaacggtgc gccaattacc gaacctgtcg ctgtagcagc tgctgctacc
    18061 acggcggcac aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc
    18121 tctgttgatg ttccaccttc gcaacctaca gtagcctggc agcctgtacc gggtccacaa
    18181 acgggagagc cggttattgc tcctgcaccg gaaggttacc cacagcagcc acaatatgcg
    18241 cagcctgcag tgcaatataa tgagccgctg caacaaccag tacagccaca gcagccgtat
    18301 tatgcacctg cagctgaaca acctgtgcaa caaccttact acgctcctgc ggcggaacaa
    18361 cctgttcagc aaccgtatta cgcccctgcg ccagaacaac cggtggcagg taacgcctgg
    18421 caagccgaag agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact
    18481 tatcagcaac cagccgctca ggagccgttg taccaacagc cgcaacccgt tgaacagcag
    18541 cctgttgtgg agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc gccactttac
    18601 tattttgaag aagtggaaga gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat
    18661 caaccgattc cagaaccggt taaagaacca gaaccgatca aatcttcgct gaaagcacct
    18721 tctgttgcag cagtacctcc agtagaaacc gctgccgctg tttctccgct ggcatctggc
    18781 gtgaaaaaag cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt
    18841 ctggcaaata gcggtggacc gcgtccgcag gtcaaagagg ggattggccc gcagttgcca
    18901 cgaccgaaac gtatccgcgt gccaactcgc cgtgaattgg cgtcttacgg tattaagctg
    18961 ccctcacagc gtgcggcgga agaaaaagcc cgtgaagccc agcgcaatca gtacgattct
    19021 ggcgatcagt acaacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag
    19081 ttcgcccaga cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgtaaac
    19141 gcagaagatg cagatgctgc ggcagaggct gaactggctc gtcagtttgc gcaaactcaa
    19201 caacaacgtt attccggcga acaaccggct ggggcgaatc cgttctcgct ggatgatttt
    19261 gaattttcgc caatgaaagc gttgctggat gatggtccac acgaaccgtt gtttacgcca
    19321 attgttgaac ctgtacagca gccgcaacaa ccggttgcac cgcagcagca gtatcagcag
    19381 ccacaacagc cggttgcgcc acagcagcaa tatcagcagc cgcaacagcc ggttgcgccg
    19441 cagccacaat atcagcagcc gcaacaaccg gttgcgccac agcagcaata tcagcagccg
    19501 caacaaccag ttgcgccgca gccgcagtat caacagccgc aacaaccggt tgcgccgcag
    19561 cagcagtatc agcagccaca acagccagtt gcgccacaac cgcaggatac cctgcttcat
    19621 ccgctgttga tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac gccgctgcct
    19681 tctctggatt tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt
    19741 gaacaaatgg ctcgcctggt ggaagcgcgt ctggctgatt tccgtattaa agccgatgtt
    19801 gtcaattact ctccggggcc ggttatcact cgctttgaat tgaacctggc accgggcgta
    19861 aaagcggcgc gcatttctaa cttgtcacgg gaccttgccc gttcactttc gacggtggcg
    19921 gtgcgtgtcg ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accgaataaa
    19981 aaacgacaaa ccgtttatct gcgcgaagtt ttggataacg ccaaattccg cgataatccg
    20041 tcgccattaa ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg
    20101 gcgaaaatgc cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg
    20161 aacgcgatga tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg
    20221 atcgacccga aaatgctgga gctttcggtt tatgaaggca ttccgcatct gttaacggaa
    20281 gtcgttactg atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag
    20341 cgtcgttata aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa
    20401 attgctgaag ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgac
    20461 agtatggatg cccagcatcc ggtgctgaaa aaagaaccat acattgtggt gttggttgac
    20521 gaatttgccg acctgatgat gacggtaggt aaaaaagtgg aagagctgat agcacgtctg
    20581 gcgcaaaaag cccgtgccgc gggtatccac ctcgtactgg caactcagcg tccatcggtt
    20641 gatgttatta ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc
    20701 agtaagattg actcacgtac cattcttgat caggctggcg cggaatcact gctggggatg
    20761 ggggatatgc tctactctgg gccgaactcc acgttgccgg tacgtgtcca tggtgctttt
    20821 gttcgcgatc aggaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag
    20881 tatgttgatg gcatcacttc cgacagtgaa agcgaaggtg gcgcgggcgg tttcgatggc
    20941 gctgaagaac tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa
    21001 gcgtcaattt ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt
    21061 atcgaacaga tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa
    21121 gtgctggctc caccgccgtt tgactaatta atgcattgcc ggataaggcg cggtagcgtc
    21181 gcatccggca ctctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt
    21241 agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt gaggaataat
    21301 gatgaaaaaa attgccatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg
    21361 ggctgatgcc gcaagcgatc tgaaaagccg cctggataaa gtcagcagct tccacgccag
    21421 cttcacacaa aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct
    21481 gtgggtgaaa cgtccaaact tattcaactg gcatatgaca caacctgatg aaagcattct
    21541 ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac
    21601 ttggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag
    21661 cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc
    21721 cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca
    21781 gtttagcgcg gtggagcagg acgatcagcg cagcagttat caactgaaat cccagcaaaa
    21841 tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga
    21901 tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt
    21961 ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggccagcaac
    22021 atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat ctgcattcta
    22081 tgatcctctg ggggccaccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct
    22141 atgcgaacgc tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg
    22201 aggcgatcga gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg
    22261 acgaagtgca ccgattcaac aaaagccagc aggatgcatt tctgccacat attgaagacg
    22321 gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac
    22381 tgctttcccg tgcccgtgtc tatctgttga aatccctggg tacagaggat attgagcaag
    22441 tactaactca ggcgatggaa gacaagaccc gtggttatgg tggtcaggat attgttctgc
    22501 cagatgaaac acgacgcgcc attgctgaac tggtgaatgg cgacgcgcgc cgggcgttaa
    22561 atacgctgga aatgatggcg gatatggccg aagtcaatga tagcggtaag cgggtcctga
    22621 agcctgaatt attgaccgaa atcgccggtg agcgaagcgc ccgctttgat aacaaaggcg
    22681 atcgctttta cgatctcatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg
    22741 cggcgctcta ctggtatgcg cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc
    22801 gtcgttgcct ggcaattgct tccgaagatg tcggcaatgc cgacccacgg gcgatgcagg
    22861 tggcaattgc ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca
    22921 ttgctcaggc gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt
    22981 ttaaagctgc gctggcggat gctcgcgaac gcccggatta tgacgtgccg gttcatttgc
    23041 gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc
    23101 atgatgaagc aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa
    23161 cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct
    23221 ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg
    23281 gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg
    23341 ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg
    23401 ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgaag
    23461 agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat
    23521 cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag
    23581 tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg
    23641 aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg ccggtaggta
    23701 aagacgaaaa tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag tttgactttg
    23761 aagttcgtga ccacgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagctgcag
    23821 ttaagctgac tggttcccgc tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg
    23881 cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg
    23941 ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg
    24001 gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga
    24061 tcccaacggc agaagttccg ctgactaacc tggtacgcgg tgaaatcatc gatgaagatg
    24121 atctgccaat taagatgacc gcccacaccc catgcttccg ttctgaagcc ggttcatatg
    24181 gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga
    24241 tcgtgcgccc ggaagactca atggcggcgc tggaagagat gactggtcat gcagaaaaag
    24301 tcctgcagtt gctgggcctg ccgtaccgta aaatcatcct ttgcactggc gacatgggct
    24361 ttggcgcttg caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgtg
    24421 agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc
    24481 gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtctggctg
    24541 ttggtcgtac gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag
    24601 taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt
    24661 ttctgaatct aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata ccgaacaata
    24721 attactcttc acttacacgt aatactactt tcgagtgaaa atctacctat ctctttgatt
    24781 ttcaaattat tcgatgtata caagcctata tagcgaactg ctatagaaat aattacacaa
    24841 tacggtttgt tactggaatc aatcgtgagc aagcttgagt gagccattat gaaaacgaaa
    24901 atccctgatg cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg
    24961 atcggcggct tggcaatggc cagcagcgca ttaacattac cttttagtcg gattgcgcac
    25021 gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag cgcctgtaca
    25081 gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga aatcaaatat
    25141 gtcgaaacgg acaataccgg cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgc
    25201 ctgcgtgggc gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa atatccgatg
    25261 aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga agaagcctac
    25321 gacatcatcg cgaccaatat gcagcgcctg atcaaagagt acggcaacga gtctatctat
    25381 ctgaactatg gcaccggtac gctgggcggc accatgaccc gctcctggcc gccgggaaat
    25441 accctggtcg cgcggctgat gaactgctgc ggcggctatc tgaaccatta cggcgactac
    25501 tcctccgcgc aaattgcgga aggtttgaac tatacctacg gcggctgggc agatggcaac
    25561 agcccgtcgg atatcgaaaa cagtaagctg gtagtgctgt ttggtaataa ccctggcgaa
    25621 acgcgaatga gtggcggtgg ggtgacttac tatcttgaac aggcacgcca gaaatctaat
    25681 gcccgcatga tcatcatcga tccgcgctat accgacaccg gtgccgggcg cgaagatgag
    25741 tggatcccta ttcgtccggg aacagatgcc gcactggtta acggtctggc gtacgtcatg
    25801 atcactgaaa acctggtgga tcaggcattc ctcgataaat attgcgttgg ctacgatgag
    25861 aaaaccctgc cagccagtgc gccgaaaaat ggccactata aagcttatat tctgggtgaa
    25921 gggccagatg gcgtggctaa aacaccggaa tgggcctcac aaatcaccgg tgttccggca
    25981 gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag
    26041 ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg
    26101 gcgattctga ccggtaacgt tggtattaat ggagggaaca gcggcgcgcg tgaaggttca
    26161 tacagcttac cgtttgtccg tatgccgacc ttggaaaacc cgatccagac cagcatttcg
    26221 atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt
    26281 gtacgcggga aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac
    26341 tgcctggtta accagcattc tgaaatcaac cgtacccatg aaatccttca ggatgataag
    26401 aagtgcgagc tgattgtggt tatcgactgc cacatgacct catcggcgaa atatgctgac
    26461 atcctgctgc ctgactgcac cgcttccgaa cagatggact ttgcgctgga tgcatcctgc
    26521 gggaatatgt cttacgtgat tttcaacgat caggtgatta aaccgcgctt tgaatgtaag
    26581 accatctatg aaatgaccag cgaactggca aaacgtcttg gcgttgagca acagtttact
    26641 gaaggccgta cccaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt
    26701 cctgaactgc caacgtttga agagttccgc aagcagggga tctttaaaaa gcgcgaccca
    26761 caagggcatc acgttgctta taaagccttc cgtgaagatc cgcaggcaaa cccactgact
    26821 acgccatcgg gcaaaattga gatttattcg caggcgctgg ctgacattgc cgctacctgg
    26881 gaattgccag aaggcgatgt gatcgatcca ctgccgatct acacgccggg ctttgaaagt
    26941 tatcaggatc cgctgaacaa acagtatccg ctgcagctta cgggtttcca ctataaatct
    27001 cgcgttcact caacttacgg caacgttgat gtgctgaaag cagcttgccg tcaggaaatg
    27061 tggatcaacc cgcttgatgc gcaaaaacgc ggtatcaaca acggcgataa agtcaggatc
    27121 tttaacgatc gtggtgaagt tcatattgag gcgaaagtga cgccacgaat gatgccaggt
    27181 gtggtcgcac tgggtgaagg tgcctggtat gacccggatg caaaacgtgt cgataagggt
    27241 ggttgtatta acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca
    27301 catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg
    27361 attttttatt gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga
    27421 ctacaaagat ttgacgccag aagtcagctt ccgccgcatt tatgaatatg ctggcggcga
    27481 ctggcaggaa gataacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc
    27541 gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa
    27601 acgtgaagat ggttttgttg tggtcgatga agatgtgtgc attggctgcc gctactgcca
    27661 tatggcttgc ccgtatggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg
    27721 cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg atctgtgttg aatcttgtcc
    27781 gctgcgggcg ctggatttcg gccctatcga cgagctgcgt aaaaaacatg gcgatctggc
    27841 ggccgttgcg ccgttgccgc gagctcactt taccaaaccg aatattgtga tcaaacccaa
    27901 tgccaatagc cgcccgaccg gggataccac tggctatctg gcaaacccga aggaggtgtg
    27961 agatgggaag tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg
    28021 tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgagcagaag
    28081 cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt gctgatgggc attggcttta
    28141 tcgcttctat gctccatctt ggttcaccga tgcgcgcttt taactcgctc aaccgggtag
    28201 gggcttcagc actcagtaac gaaatcgcca gcggttcgat cttttttgcc gtaggcggca
    28261 tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc attgcgtacg ctttggctgg
    28321 tggtgacgat ggttcttggc gtcgtctttg tctggatgat ggtgcgtgtg tataacagca
    28381 ttgataccgt tccgacctgg tacagcatct ggacgccgat gggcttcttc ctgacgatgt
    28441 tcatgggcgg cccgttactg gggtacctgt tgctgagtct ggctggcgtc gatagctggg
    28501 cgatgcgtct gctgccagcc atttccgtac tggcactggt ggtgagtggc gtggtgtcag
    28561 tgatgcaggg cgcagagctg gcgactattc atagttctgt gcagcaggcc gcagcgctgg
    28621 tgccggacta tggtgcgctg atgtcctggc ggatcgtgct tttggccgtt gccctgtgct
    28681 tgtggattgc accacagcta aaaggttatc agcctgcggt tccgctacta tccgtctcat
    28741 tcattctgct tctggcaggg gaattaatcg gtcgcggcgt attctatggt ttgcatatga
    28801 ccgtggggat ggccgtcgca agctaatcag taacaaccgg ggtttcggcc ccggttctct
    28861 tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa tcaggaatat
    28921 ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcacgg tgcagctcgc
    28981 aagccacgcc gaaccatgtc atcaattgcg cacctgcttg cgacatgcga tcccatgccg
    29041 aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacaacaaac acatcaaacc
    29101 cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa
    29161 taattaactg ttttttacct gtcgctttga ctgcttttac aaaatcttcg ttatcccagg
    29221 cgttaatatt gcccgggcga gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg
    29281 gaactaatgg gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga
    29341 aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat
    29401 cccgtacaag ggaaagtaaa ccagcctggt gatcaacaag caaaacagca gcatcatttt
    29461 tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgcc
    29521 tgaaatcggt cagacgtgat gcattgctct gaaagcatag acgggaaata tgagtttgct
    29581 gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttaga tcaataaaaa
    29641 caatcagatg agattaatca tctcttcagt attagcgtta tgaatgagta atataaatcg
    29701 ataataccgc cgttgcgata tgcgcgtatt ttcacccatt gacaatgttt ttggcggtgg
    29761 catgatgcgc atgaaatttg aacttcctca cggttttaat tcatgtccac gtatacccgg
    29821 cctgtcatgc ttttgctgtc tggcctgctt ttgttgactc tggcgatcgc ggtgttaaat
    29881 acactcgtgc cgctttggct cgcccaggaa cacatgtcca catggcaggt aggcgttgtc
    29941 agctcatcct attttaccgg caaccttgtc ggtacattgc tgacagggta tgtcattaaa
    30001 cgcattggtt ttaaccgcag ctattatctg gcctccttca tttttgccgc tggctgtgcc
    30061 ggccttggcc tgatgattgg attctggagt tggttggcgt ggcgttttgt cgcgggcgtc
    30121 ggctgtgcca tgatttgggt agtcgttgag agcgcgctga tgtgcagtgg gacgtcacgt
    30181 aaccgtgggc gtttgcttgc tgcatacatg atggtttatt acgtgggaac gtttttaggc
    30241 cagttactgg tcagcaaggt ctcaaccgag ctgatgagcg tattgccgtg ggttacaggt
    30301 ttgacattgg cagggatctt accgctgttg tttacgcgtg tcctgaatca gcaggctgaa
    30361 aaccatgatt cgacgtcaat tacggcaatg ctaaaactcc gtcaggcgcg gcttggcgtg
    30421 aatggctgca ttatctcagg aatcgttctg ggatctctat atggcctgat gcctctgtac
    30481 ctcaatcaca aaggggtgag caatgccagt attggtttct ggatggcggt actggtcagt
    30541 gcgggtatcc tcggacaatg gccgattgga cgtctggcgg ataagtttgg tcgactgctg
    30601 gttttgcgtg ttcaggtctt tgtcgtcatt ctcggcagta ttgcgatgct tagccaggcg
    30661 gcgatggccc cagcgttatt catcctcggt gccgctggct ttacgctata tccggtggcg
    30721 atggcatggg cttgcgagaa agttgaacat catcaactgg tggcgatgaa ccaagcctta
    30781 ctgttgagct atactgtggg aagtctgctt ggcccgtcat ttaccgctat gctaatgcag
    30841 aatttctccg ataatttatt gtttatcatg atcgccagcg tatcgtttat ctatttgctg
    30901 atgctgctgc gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta aatgaattca
    30961 agcagagtgt gaacttactg tttcacactc tgcttttttg tttcttctat ctgacttgct
    31021 ttattccaaa ttttattcgt ttaaaaataa aatgtgtagc aggttataat tttgcatttc
    31081 gctatttccg cacttcttat ttgccgcgca taatccctcg ttttacagat gcccctataa
    31141 ttttggcgaa ggatttgtct atggctggga atgttcagga aaaacagctg cgatggtaca
    31201 acattgcgct gatgtctttt atcactgtct ggggttttgg caacgttgtt aataactacg
    31261 ccaaccaggg gctggtggtt gttttttcat gggtgtttat ctttgcgctc tatttcacac
    31321 cttatgcgct aattgttggt cagttaggct cgaccttcaa agatgggaag ggcggggtca
    31381 gtacctggat taaacacacg atgggacccg gactggctta tctcgccgcg tggacctact
    31441 gggtggtgca tattccctat ctggcacaaa aaccctaggc aattctgatt gcgctcggtt
    31501 gggcgatgaa aggcgacggt tcgctaatca aagaatattc agtcgtagcg ttacaggggt
    31561 taacgctggt gctgtttatc ttctttatgt gggttgcttc acgcggtatg aaatcgctga
    31621 aaatcgtcgg ttctgtggca gggattgcta tgtttgttat gtcgctcctg tatgtggcga
    31681 tggcggtaac cgcgcctgca attactgaag tgcatattgc gaccacaaac attacctggg
    31741 aaacgttcat tcctcatatc gactttacct acattaccac tatttcaatg ctggttttcg
    31801 cggttggcgg agcagagaag atttctcctt acgttaatca aacgcgcaac ccaggaaaag
    31861 aatttccaaa agggatgtta tgcctggcgg tgatggttgc ggtttgtgcc attctgggct
    31921 cgctggcgat ggggatgatg tttgattcgc gtaatatccc ggatgactta atgaccaacg
    31981 gtcagtatta cgcctttcag aagctgggcg agtattacaa catgggtaat actttaatgg
    32041 tgatttacgc cattgcgaat accctgggac aagtagcggc gctggtattc tcgattgatg
    32101 ccccgcttaa agtgctatta ggtgatgctg acagcaaata tattccagcc agtttatgtc
    32161 gtaccaacgc ttctggtacg cccgttaatg gctattttct gaccctggta ctggtggcga
    32221 ttctgattat gctgccgact ctcggcattg gtgatatgaa caatctctat aaatggctgt
    32281 tgaaccttaa ttcggtagtg atgccgctgc gttatttatg ggtatttgtt gcatttattg
    32341 cagtcgttcg cctggcgcag aaatataaac cagaatatgt ctttattcgt aataagcctc
    32401 tggcaatgac cgtcgggatt tggtgttttg cctttaccgc ctttgcctgt ttgacgggga
    32461 tcttcccgaa aatggaagcc ttcactgcag agtggacctt ccagttggcg ctgaatgttg
    32521 caacgccgtt tgtgctggta ggattaggac tgatattccc gctgctggcg cgtaaagcga
    32581 atagtaaata attattgtgg tcggtgacgc tgactctggc agatctgacc cgtttttaca
    32641 taatcgagaa atgcgcgcaa gccgcaggac atatactggc ggtttgggta atagatctgg
    32701 aagcctggac gctctgtgat ccagtcttcc agtacacatt ctaaacgacc ggtatcaagg
    32761 tactccttga tctgttcata gagcagatac ccgataccag cacccatcaa aacggcatcc
    32821 agttcagcat caacatc
//
LOCUS       DAETQN010000038        32586 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.038, whole genome shotgun sequence.
ACCESSION   DAETQN010000038 DAETQN010000000
VERSION     DAETQN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32586
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.038"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            520..759
                     /locus_tag="K0W68_001769"
     CDS             520..759
                     /locus_tag="K0W68_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HBI3073349.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(947..1924)
                     /gene="intI2"
                     /locus_tag="K0W68_001770"
                     /pseudo
     CDS             complement(947..1924)
                     /gene="intI2"
                     /locus_tag="K0W68_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2256..2729
                     /gene="dfrA"
                     /locus_tag="K0W68_001771"
     CDS             2256..2729
                     /gene="dfrA"
                     /locus_tag="K0W68_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA"
                     /protein_id="HBI3073350.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2824..3348
                     /gene="sat2"
                     /locus_tag="K0W68_001772"
     CDS             2824..3348
                     /gene="sat2"
                     /locus_tag="K0W68_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HBI3073351.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3417..3914
                     /locus_tag="K0W68_001773"
     CDS             3417..3914
                     /locus_tag="K0W68_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073352.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            3975..4346
                     /locus_tag="K0W68_001774"
     CDS             3975..4346
                     /locus_tag="K0W68_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073353.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            4756..5133
                     /locus_tag="K0W68_001775"
     CDS             4756..5133
                     /locus_tag="K0W68_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073354.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            5156..5308
                     /locus_tag="K0W68_001776"
                     /pseudo
     CDS             5156..5308
                     /locus_tag="K0W68_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5364..6980)
                     /locus_tag="K0W68_001777"
     CDS             complement(5364..6980)
                     /locus_tag="K0W68_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HBI3073355.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(6981..8507)
                     /locus_tag="K0W68_001778"
     CDS             complement(6981..8507)
                     /locus_tag="K0W68_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HBI3073356.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(8510..10177)
                     /locus_tag="K0W68_001779"
     CDS             complement(8510..10177)
                     /locus_tag="K0W68_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI3073357.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(10174..12282)
                     /locus_tag="K0W68_001780"
     CDS             complement(10174..12282)
                     /locus_tag="K0W68_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBI3073358.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(12269..13090)
                     /locus_tag="K0W68_001781"
     CDS             complement(12269..13090)
                     /locus_tag="K0W68_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HBI3073359.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(13250..15079)
                     /gene="glmS"
                     /locus_tag="K0W68_001782"
     CDS             complement(13250..15079)
                     /gene="glmS"
                     /locus_tag="K0W68_001782"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBI3073360.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(15241..16611)
                     /gene="glmU"
                     /locus_tag="K0W68_001783"
     CDS             complement(15241..16611)
                     /gene="glmU"
                     /locus_tag="K0W68_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBI3073361.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(16962..17381)
                     /gene="atpC"
                     /locus_tag="K0W68_001784"
     CDS             complement(16962..17381)
                     /gene="atpC"
                     /locus_tag="K0W68_001784"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBI3073362.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(17402..18784)
                     /gene="atpD"
                     /locus_tag="K0W68_001785"
     CDS             complement(17402..18784)
                     /gene="atpD"
                     /locus_tag="K0W68_001785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBI3073363.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(18811..19674)
                     /gene="atpG"
                     /locus_tag="K0W68_001786"
     CDS             complement(18811..19674)
                     /gene="atpG"
                     /locus_tag="K0W68_001786"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBI3073364.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(19725..21266)
                     /gene="atpA"
                     /locus_tag="K0W68_001787"
     CDS             complement(19725..21266)
                     /gene="atpA"
                     /locus_tag="K0W68_001787"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBI3073365.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(21279..21812)
                     /gene="atpH"
                     /locus_tag="K0W68_001788"
     CDS             complement(21279..21812)
                     /gene="atpH"
                     /locus_tag="K0W68_001788"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBI3073366.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(21827..22297)
                     /gene="atpF"
                     /locus_tag="K0W68_001789"
     CDS             complement(21827..22297)
                     /gene="atpF"
                     /locus_tag="K0W68_001789"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBI3073367.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(22359..22598)
                     /gene="atpE"
                     /locus_tag="K0W68_001790"
     CDS             complement(22359..22598)
                     /gene="atpE"
                     /locus_tag="K0W68_001790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBI3073368.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(22645..23460)
                     /gene="atpB"
                     /locus_tag="K0W68_001791"
     CDS             complement(22645..23460)
                     /gene="atpB"
                     /locus_tag="K0W68_001791"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBI3073369.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(23469..23849)
                     /gene="atpI"
                     /locus_tag="K0W68_001792"
     CDS             complement(23469..23849)
                     /gene="atpI"
                     /locus_tag="K0W68_001792"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBI3073370.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(24466..25089)
                     /gene="rsmG"
                     /locus_tag="K0W68_001793"
     CDS             complement(24466..25089)
                     /gene="rsmG"
                     /locus_tag="K0W68_001793"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBI3073371.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(25153..27042)
                     /gene="mnmG"
                     /locus_tag="K0W68_001794"
     CDS             complement(25153..27042)
                     /gene="mnmG"
                     /locus_tag="K0W68_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBI3073372.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(27421..27864)
                     /gene="mioC"
                     /locus_tag="K0W68_001795"
     CDS             complement(27421..27864)
                     /gene="mioC"
                     /locus_tag="K0W68_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBI3073373.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(27954..28412)
                     /gene="asnC"
                     /locus_tag="K0W68_001796"
     CDS             complement(27954..28412)
                     /gene="asnC"
                     /locus_tag="K0W68_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBI3073374.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            28564..29556
                     /gene="asnA"
                     /locus_tag="K0W68_001797"
     CDS             28564..29556
                     /gene="asnA"
                     /locus_tag="K0W68_001797"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBI3073375.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(29561..31012)
                     /gene="viaA"
                     /locus_tag="K0W68_001798"
     CDS             complement(29561..31012)
                     /gene="viaA"
                     /locus_tag="K0W68_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBI3073376.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(31006..32502)
                     /gene="ravA"
                     /locus_tag="K0W68_001799"
     CDS             complement(31006..32502)
                     /gene="ravA"
                     /locus_tag="K0W68_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBI3073377.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         8313 a         8237 c         7555 g         8481 t
ORIGIN      
        1 tcggcagccc tgtgggcgga caaaatagtt gggaactggg aggggtggaa atggagtttt
       61 taaggattat ttagggaaga gtgacaaaat agatgggaac tgggtgtagc gtcgtaagct
      121 aatacgaaaa ttaaaaatga caaaatagtt tggaactaga tttcacttat ctggttaact
      181 aatctagcta acctgccgct cagcttagta cgatcctata tccaactgat cataatcctt
      241 tcatcagtat cccaaattaa atccccctcc tagactgcct caccaacact ttacagtgat
      301 cccgtatttc cggacaggtg attgattttt ttcgcttgta caggtgttaa gccacagtta
      361 tcttgatgtg gacattatgc attgtaatag cccattaaca tgcgctaaga gcataaatta
      421 atatacagta acaagcatta cgaggtgcta atgtttggaa agcagcctct tccaactaca
      481 ataataagtt aactatcctc cacccacaaa taattacaaa tgaccccttt ccgctataac
      541 tccgatctca ccagcggctc gctacaaacc cgagagtgtc gcattattac tggcttgtta
      601 ctccaagagc ttgatgaagc tgcctgggat aaagccatgt ataaggaaaa tgtgcttcag
      661 aaacgcacgc aatctacggt aagacgtatt tcttcagcgc ttagaaaacg cctagaacac
      721 ctaagctctg acttttgggc ttttgcgttt ttatgttagg cattgtctgg caactggagg
      781 aagtgtaatg accatgaatg gtcactgtga ttgacagtat cttgcttaaa cttatctgaa
      841 caatggtaga actggctcat atttatcccc tctatgattg tttatctttt tcaccataaa
      901 aagtatcagg ggctactcag ggctacataa agatatttat ctacttttac tgattgataa
      961 gtagcatcag tccatccgca ggactggtgg tgccggcaaa atgctgaccc aacacatgcg
     1021 tatagatttg cgtggtctta acatcgttat gccctaagag ttcttgcaca gtgcgaatat
     1081 cacgccccgc ttgtaataga tgcgtagcaa acgagtgacg aaatgtatga caagtgacac
     1141 gcttgctaac gatgcctgct ttttgtacgg ctgccttcaa tgcctttcgc gcaacggagt
     1201 catgcagatg atggcggcat aatttgccgt tatacgggtg gttgcagagc gtgctggagg
     1261 gaaagacaaa catccacgcc gcttgtcgat aagcagaagg gtatttgtga tctaaagcaa
     1321 aaggcagcga tggccctacg ccttgtaagt tgtcgtcttg ctgaataagc cgcgcttgct
     1381 caatgagtta ttttattgct gggattaggc gcgtgggcag taggctgttt ctgcttttcc
     1441 cacccttacc gtcatgcaca gtgatgcagc cattatcaaa atcaaaatct ttaacccgca
     1501 aacgcaagca ttcattaatg cgcaaacctg caccatacag cagcgtaaaa ataacttggt
     1561 tgcgagtatc cataacctgc aaaatgcgtt gcacttcatt tgcagagata acagagggta
     1621 gccgtctagg cttgcttgca gggatataat caatatcgcc caacggctgt tgtaaaaacc
     1681 tgttgtacaa aaaagctagg gcatttaaag cgattttctg cgtgtttatg gctacatgtc
     1741 tgctgtttgc taagctggat aaaaacagcc tgacctcttc actgcccatg gtctgaggat
     1801 gacgtttttt gtgaaacaga ataaaacgct taatccagtg caggtaagtt ttttcagttt
     1861 tcagcgcata acctttttgt cgcatatccg tgcgtataga atttaaaaat ggactgttag
     1921 acataaaacg ctccttgtct tgcaactgtc tgcctataca gcctattcta gctgggattt
     1981 aaaaaagtgc ctgtttttta cgcctagaga tgcttgttta ccggtagagt tttaatttaa
     2041 tgctaaataa attaaaatgt tatgagttct ttgggtgaga taatgtgcat cgtgcaagca
     2101 ggatagacgg catgcacgat ttgtaataac agagtgtctt gtatttttaa agaaagtcta
     2161 tttaatacaa gtgattatat taattaacgg taagcatcag cgggtgacaa aacgagcatg
     2221 cttactaata aaatgttaac ctctgaggaa gaattgtgaa actatcacta atggtagcta
     2281 tatcgaagaa tggagttatc gggaatggcc ctgatattcc atggagtgcc aaaggtgaac
     2341 agctcctgtt taaagctatt acctataacc aatggctgtt ggttggacgc aagacttttg
     2401 aatcaatggg agcattaccc aaccgaaagt atgcggtcgt aacacgttca agttttacat
     2461 ctgacaatga gaacgtattg atctttccat caattaaaga tgctttaacc aacctaaaga
     2521 aaataacgga tcatgtcatt gtttcaggtg gtggggagat atacaaaagc ctgatcgatc
     2581 aagtagatac actacatata tctagaatag acatcgagcc ggaaggtgat gtttactttc
     2641 ctgaaatccc cagcaatttt aggccagttt ttacccaaga cttcgcctct aacataaatt
     2701 atagttacca aatctggcaa aagggttaac aagtggcagc aacggattcg caaacctgtc
     2761 acgccttttg taccaaaagc cgcgccaggt ttgcgatccg ctgtgccagg cgttaggcgt
     2821 catatgaaga tttcggtgat ccctgagcag gtggcggaaa cattggatgc tgagaaccat
     2881 ttcattgttc gtgaagtgtt cgatgtgcac ctatccgacc aaggctttga actatctacc
     2941 agaagtgtga gcccctaccg gaaggattac atctcggatg atgactctga tgaagactct
     3001 gcttgctatg gcgcattcat cgaccaagag cttgtcggga agattgaact caactcaaca
     3061 tggaacgatc tagcctctat cgaacacatt gttgtgtcgc acacgcaccg aggcaaagga
     3121 gtcgcgcaca gtctcatcga atttgcgaaa aagtgggcac taagcagaca gctccttggc
     3181 atacgattag agacacaaac gaacaatgta cctgcctgca atttgtacgc aaaatgtggc
     3241 tttactctcg gcggcattga cctgttcacg tataaaacta gacctcaagt ctcgaacgaa
     3301 acagcgatgt actggtactg gttctcggga gcacaggatg acgcctaaca attcattcaa
     3361 gccgacaccg cttcgcggcg cggcttaatt caggagttag agagctgggg aagactatgc
     3421 gcgatctgtt gaaggtggtt ctaagcctcg tacttgcgat ggcatcgggg caggcacttg
     3481 ctgacctgcc aattgtttta gtggatgaag ctcgtcttcc ctatgactac tccccatcca
     3541 actacgacat ttctccaagc aactacgaca actccataag caattacgac aatagtccat
     3601 caaattacga caactctgag agcaactacg ataatagttc atccaattac gacaatagtc
     3661 gcaacggaaa tcgtaggctt atatatagcg caaatgggtc tcgcactttc gccggctact
     3721 acgtcattgc caacaatggg acaacgaact tcttttccac atctggcaaa aggatgttct
     3781 acaccccaaa aggggggcgc ggcgtctatg gcggcaaaga tgggagcttc tgcggggcat
     3841 tggtcgtcat aaatggccaa ttttcgcttg ccctgacaga taacggcctg aagatcatgt
     3901 atctaagcaa ctagcctgct ctctaataaa atgttaggcc tcaacatcta gtcgcaagct
     3961 gaggggaacc actagtgtca tacgaacctc caagagacgg ttacacaaac gggtacattg
     4021 ttgatgtcat gtatgacaat cgcccaagta agtatccagc tgtgttcaga acgtacgtcc
     4081 gaattccaga ctatgtcgaa ctagataacg cggaggagct ggccgacgca atcattcgag
     4141 aggttgagtt aaaggaggca tggctcacaa gggtcttggg gaactcagcc gatcctgacg
     4201 cttctgtggg caagtgggac cggcgcctag cgcagtgcgg tggtattgat gagatgtcaa
     4261 gtggggtgca ggcaacatac ttcagtccaa acggtcagct ggtcggctta gtggcataca
     4321 ttgatgccga atggatggat gaatagctct gacgttacga ttcaggtgag acaaccagat
     4381 gggcattgcg tacaagctca gttgccaccg atgtggctac gagtctgatc ttctttacct
     4441 aggccaaggt atggcgatgc ttcctgagca aatagtcgga acgtgcacat gctgcgacaa
     4501 tctaacgacg atttctataa atgaaagcta cggagtatgc tcactttgtg gggctaagga
     4561 tcgtgtgata tttacaagct atcagcatga ggcaaggcca agaaatccct gctatctaga
     4621 gagaagtttc aaatatcaat gcccaagatg ccatgccttc agtatggagc ctccggatct
     4681 accggagatg ctttgggact aatgctccgc tttgcctcgg gtatccgttc gacctatgtt
     4741 taagaggaaa cgcacgtgtc tgccgccaaa gctcttgcct atgcactttg tgacaagttg
     4801 gaaagcaaaa aagttgatga agtcgtacga aatcttgttt ctagcggctg gaatattagg
     4861 gaagtggcct gggaagcttc gtcctttgat catgaaatgc cctactttga cacggggcgg
     4921 gacttcgata ttggatttcc tgacacgctg tcatccaaga ttgagttttt gaccgcgaat
     4981 ggatcaatcg tccaagcacg tgctcgcctg cacttcaagc aggctctaat ttttgccaag
     5041 gcgtccaaac atgtcgccgc gctcaagaac tcattggatt actactatgg atcgtctgta
     5101 atcccccctc aaaaccacgg aggctatttg tagaaaattc cattaatatg aactcaaggt
     5161 gttccctacg gacgaatcag cgatgaaggt ggtgtacctg gctatccagg cggcgaccaa
     5221 gaaatggacg atgccgatcc gcaactggaa gccggccatg aaccgtttta tgatcgaatt
     5281 tggtgaccgt ttaaatggcc acctttaact gaccggcaaa agcacttaca caaaagggtt
     5341 tacaggctct ttcagtcatt cctttaatgc gtaaattgct ctctaaccac tctctcagcc
     5401 cacccttcca tatcctctct cgatacttgc agctccccca acccctttgg atgattcacc
     5461 cctctatgcc cgtcttgtcc atataattga tcaaacaaac tatttggcca attcaatgag
     5521 ctcttcacca ctccacgccg tatcttttca aaatcattcc gccatgtttc gctatcaacg
     5581 ccactcaaca ctttggtaga aagcattttc accccatccg atgcatccac ctctagcaat
     5641 acaaattcac tgccattacg ttgcacccca acggctttaa tcactcttgg tgagccatct
     5701 ttacacagat gtaatcggct acgcccaact tttggcaaaa ccaaggtttc ttcaaaaagc
     5761 acccgacatg caaatttagt ttttagaatt gaaagtagct catcaaaagc agcaaatcga
     5821 ttagcaaaaa tagagttgta attggttgca tcctgcttcc cacccacatc tgccgctgct
     5881 aggacaccac ctaagtgtgg ctcatcagta ctaaccaaat tactattttc acttgttgtt
     5941 gcttcttctt tttctaaaat agtttggcta ctttttcttg ccctgcgaga tttttcaatt
     6001 cgactaggtc tactaaaact tatccatgtc ggctcagcct ctatgactcg tgtgtctgtg
     6061 tctatattgg ctgtttcctc atcgtccaac tgtagatgct catcatcaac aactgactct
     6121 gttgaaaccg ctatgtgctg cgtactacca tcacctgcct ccttttcctg aaaagaggca
     6181 tggctaatag ctgttgtgct aggaagcata gcgttgattt ctaaacctac tatttcctca
     6241 actaaataat ccttatcctc gttagaagaa cgtcctttta catgtaattt ccaaccctgc
     6301 atgggcggag ggtcaaagct aaagcaccag ctttcaacgc cgttcttaat ttctctattt
     6361 tgttgatagt gcctaaaaat actttcatac gaatccataa catcttgatc cgaaaacaac
     6421 caaaccaaaa gctgcactac ggccgactgc tctaacgccc ctttaggaaa cgatgaactg
     6481 ggtaagatcc ttatctctaa atgatctcgc tcaacctcat actgaacgtc gaactcttgc
     6541 tgtaaagcgg tactgctcaa acagcttcga cagaaatagg agttaattaa aaataatgac
     6601 cgagctagct ctaactgtgg gatgtgataa cgagtcttgc tatatggtgt ttcagcttcg
     6661 taaataaatg aactctgcgc tccatccttg ttgcgtacgg aagcaaagga cgagagatta
     6721 ggaaaatcga ttattttggc tcgttgaaga tccgatgctt caaactcaat cacacgatcc
     6781 gccttattta tttgctttgt tgtactgtta accaccttag ctcgacttaa taaaggcaaa
     6841 tgagtaaatc gagtccattt ccgttcttgc attggattaa accaaacaaa aatacgccac
     6901 tccttatgac ccgagttacg gaataaatga ccaatatgta caacctgcac attgtcatta
     6961 aatgtagcta gcctaaccac tcactcttcc ccataaacca tttccaagaa tctttgtgct
     7021 tcctcagtta tccgctcttt agataacgtt gcacttctta ataatcgcca gcgcctaagc
     7081 tcaacatctt cctgtttaag cttaataaaa gcttggctaa tccgtctaat ttgataatct
     7141 tccacactct ctgagtaacg ctttaagcaa agcgctacca aaggcagttt ctgtagattt
     7201 tttgcaagag aggttccgtt aggagtttgc tttaacagcc agctcgatgt cgctcttggg
     7261 tgatcaaggc tactatctag acgctttatg attcttaata gttgtcgtac agcaattcga
     7321 tctctttggt tccaatcaac tctaggggcg ggtgccagac gctcttgttg atgctgttga
     7381 ttccagtgaa ctagccaatc cctgtcatgt cggtaaagcc aagcgtatag caccccaccc
     7441 tctaaagact gtcttgccgc cttaattcct tggtatttat gcactagttg ctgccagtct
     7501 ttatgcttaa cagataaatc ttcagagtta ggttgcacag actggctaac aggtcttgtc
     7561 gttatagagt gctcagtaag agcacttgcc tgctgtagcg cttctataac cgttagtttt
     7621 ggcaataagg cttgccacac aatactatgc tgtaaataac taaaggcttt tctatgctta
     7681 cggaatatac ttttcagcca acacgtgtcc ttgttctctg ccaactttaa atccagtttc
     7741 tctagtgcct catcactaaa agtctgcctc actctctccg ccaccaagtc atgacgaatg
     7801 tgcttgcttt tggttagccc tagatcctgc gctaagcgct gataaaacag cgtccactgc
     7861 tcaaggcttg gggaaagctc ttgcgctcgt ggagcatcta acagaggggc tatataagca
     7921 gctagtgctg ttaattgaga tagggagtct ttggggtagt ctgaaagcag ctcagtatga
     7981 cccaaagccc aaaattgatg tcggtgatca tctacagctc tatcaaagaa gactaaagca
     8041 ccgtgttttg gacaatatgg caaagcgggc aaataccaat ctcgttgcca aaaggcttcc
     8101 ccatacctat ttagctgaag agcaacgcaa tcagggcagt atctaaagcg gttatcgctc
     8161 ttaactctag aagcagcgac tcctagcatt aaatgcaccg caccttgcgc ttggtactcc
     8221 attaaccgaa tagcttcgtc tcggcgctcc ttgcctacaa acggagcata taaagggaat
     8281 aaggtatgct cataaataag ctgctgaaca gcgtaacgtc ctgtttgatg tagatgtctt
     8341 gctatcacac ctaaatgcga gggcagacct aaggtagcga ccaccttgcg gttgccatac
     8401 acctcatcca acagctgctt aggactaaca atcccttgat aaacgcctgc ccgtgcaata
     8461 gtgctataaa tcagctcatt cgagtacgga acaggaaagt ttctcatggc taacctgctt
     8521 gtttaaataa gctcgccata tccactatta cccctttccc ttttaaccgt tcatgcatgg
     8581 ttttttcagg ttggcgttgt gaatagatat aacgtaaatc cgtatcaggc aagctatccc
     8641 agtcgctggg cttgactacc tttatagccg aaaccttttt actcttggag ggcttttcta
     8701 gttctgacac taccgtttcg ccttccatca accactgcaa aacaagaggc agtaactttt
     8761 gtcttgtcat cgttggattc tggctaaacg cttttttaat agtgggaatt aacaggcttg
     8821 aatcgtaatc ctctttcagc atcagatata aatgacgctg atcttcggta tctaactctt
     8881 gaagggcttt ttcttctggt gtttgttctt gtatcgctgc gatatctagc tgaagttgga
     8941 ttaaccgttt atcaatctcg ggaacgacta gatcagaata acgagcaatg cgttctggga
     9001 tacccgagcg taatgcctct agcatggggt gcacaggctt taactcatct tgatacactt
     9061 gccgcaataa accagcggta atacgctcat tgcctaaagc tagcgcacgg agctgagcga
     9121 gtacaaaaag ttttactaca atgtccatca ctccttggct tagctcatac cacacatcac
     9181 ggacctcatc cgataacagc gcatctttgc gttgtaaaag ctgtaattgc caaagattat
     9241 ccgtaaaagc gatccactct tgattgggct ttccacgttg cgtttgttgt ataggatccc
     9301 agaatatagc tccaaaccct gcccctctac gtgcagaccg caaatcagcc tcaaaaatct
     9361 ctcgtgcttt aggggtacca atcaacatca ctggtacgcc aataatattc accatcgtca
     9421 caaaaaagtt cagcatctct tgagatccac ccgaacgaga gcggcttaaa tgctgaattt
     9481 catcaataac caacaaccct aaagcatgtg cattggctat ttgcgacatc aaagccaaca
     9541 tggtttctat accatgacgt tttaagccat aacgacgctc atagttcgag cccaaggctc
     9601 gatccaacgc tctgaaaaaa ttcaagcaga tttcttttag cgaaccatta tgcgagcagt
     9661 ctattttcaa atacaccacc tgctctacat tgagttcacg atggtaaatc acctgaggat
     9721 acgtggctag aatacgatga agagaggtcg tcttcccact accagaacaa ccaattaaca
     9781 ataagctttg tgccgtagat cgtgcctcct caaagcgaaa tgtctccaac tctcccgttt
     9841 gaacacgctc ataaccattt tgtaaatgct tttgtaaatc tcctgtttta ggatttctgc
     9901 ctacgtagcc acctcgaatc atgaccgaaa tacgctcact tagtagcaaa tgcgtaccta
     9961 atggctgaaa atagtcatct ggaatacgac aaatggtatg agctctgata acacgggact
    10021 tttgcaagtc agaggaagta agctgtaaag aggatttcag tgatgcagca ctattcactg
    10081 actcttgtaa tggtggtaag gcctcgataa aaggattatc acgataagcc tctacccccg
    10141 tatcacgata aactgcttga atccgggtag cactcatgac tcatcctttt ctggtggatc
    10201 ctgaaataat tcaggcacgt atgtgggtag gctgtaatct tcttgatcat ccgcttccac
    10261 tgcgttgaaa ggaataactt tagcctcatc acctgaagag cttggcttca aatgctccgc
    10321 acgtttttta cgctccgagg tcacggcttc ttttttatta gttttaatct gcttaatgcg
    10381 tgttgatttg ggctcagtag tactgggcgt taacttattc gctttctgaa tggtttgctg
    10441 aataaacgcc tcaagctccc tgcgtttagt taactcatcc tgcttcgcat tggctttatt
    10501 gtgtttttct tgtgcttgta tatcccaaac ctcccaaaat gagagacctt taaactgccg
    10561 actacgttcc gtcagattac agcgccaaaa tacacggctg ccaacttgcg gaaacaaata
    10621 aatcgtatca accagcactg ggtcataagc cgcttctaaa tgttgaggtc tagctatatc
    10681 agtgctccgc tgcaaccaac cctcacgcag aatctctgac cccgagtaat acaaacccca
    10741 caaattaacg ccaaatgaag aaatagagac ctttcggcga ggcagtaacg ctactcgcaa
    10801 ctgctcttgc tccacagccc ttaaactacc tgtacgatgc tgcatacccc attgccatag
    10861 ctggacagga atagacggta aatctgtagg aaaatcagca tctcgatcgt atttatccat
    10921 caccagatgg ttatttctga ataagatcgt acgcaaaata atttgtgtga actcaaatac
    10981 cgacagagat gcatctaacc tatagtctgt ttcaccatgg cttttgatcc gactgccctc
    11041 tacaatgcca ggtgcaaagg acttaaactc ggcttgtagt gttctaaaag tgctttccac
    11101 tatgccttta gcatcgccac gtctaggtgg agcactttcc actcgcacat taaaactaga
    11161 aactaaggct tcgacctgat gactcattaa ttcgccacgg tccgctagca acacatctgg
    11221 caaacctaca cacggccagt ctgagctact aatctcaata tcatgctggg cacaaatggc
    11281 cgttttgtca gagcaagcat ttacaaaagc ctgcatcgcc accacataag acggattttc
    11341 aaagccgata taaaagcccg tgatcatccg actaaacaca tcaatcacaa tgtaaagcgt
    11401 tggtcttcct atgatttttt ggcgatcatg atgatccact aaataaatat cagcaatcgt
    11461 ggcatcaatc tcataacgac tcccagggcc taacgcctga gaagtggctg tactacttaa
    11521 gggtcgtacg tcttttttat atacccctgc tttaactcta gactttaagc gctgagcttt
    11581 agggtattct cgatcataaa aataacgaaa ctgacgtagt gttgggtaat cctcttgggg
    11641 aatgcgagga aaatactgag caaacaagtc cacaaatcgt ctataggcaa cggtggtctt
    11701 tgtacctttt tgatttaaca ggtgcttttc tatggtcaac ctaaaaaggc gttcaatctc
    11761 gggcgttacc ttggttcctt cacccttacc atattctctg gctcggccaa tctttgctgt
    11821 tcctgtcgct gaacgtcttt cccctggtgc accgctgttt ttgtagtcag gaattaatgc
    11881 attaggcgtt tgaccacgct gccagtaacg gcgtaacaac ttataaactg tagccttagt
    11941 aaccttatgt tcttggacca catgctcaac gagttcgctt ctgactttag ggtcgaaacg
    12001 atccttacta ttaataatag gaagaatttt tcgataatcc tcctcgcgct tctggaagct
    12061 gacagaatct acagacggct cttctaaatc aagatgtaca taagggtcat cagttctaac
    12121 taagcggcct tcatctaaat actgcattag caacaactca gccctagctt gtggaactcc
    12181 tttatcagcg cttatttgca tccagacaac ttggccatcc tctatagcca aaatgcgata
    12241 cggatcatta tcaaatagca caacctcatt aatttgccac atagcgcaac tcctccatat
    12301 tcactacttg gctaatacag agatctgcac acttatttgc cctgaaagac ttataaatat
    12361 tgaacttaat aaaaccattt gcggttaagg ctcgtatctc actcaatgtt ttgcctaact
    12421 ccaaatcata agcaatatca acctgcttac agacattgat aatgttttca tctccttttt
    12481 cttgcaggat atgggccaat ggggatagtt gtgctaaaag ctccgcagaa acttcttctg
    12541 ttttcactga ataaagccat tcaatatttt cttttactac gggatttatt tctttatcag
    12601 taaaaatgaa ccaaggaatt tgcttttgct gccaatagcg acgctctagt tctagttttt
    12661 ctaaggtacg ctcgtcttgt aaggctgctg caggtttgac ttgaatagca aactgctcaa
    12721 aaggaccatc tttgcagtcc actaaaaaat cagtagacat aacctgatct acaccacgaa
    12781 taacaggatg cttaatacca ctatctattg caatctgcct ggtatcacta ggtaataagg
    12841 ggaactgctc gcgtatatct agcacgctgc tctcccactc aagactgaga aaaacagcaa
    12901 gctctaagtc agatagcaaa tgatggactc gtcccgtctt atgagaataa atacggtggg
    12961 aacgacctga agaaggaact tcttgtactg ttagccatgg aatatagtct ttaccatgcc
    13021 cttggccacg cccctcttta atacgacggg caatttgcac ttcagaaaat gaagagtttg
    13081 ctttagccat aacaaaagtc cagtatgctt tttcacagca taactggact gatttcagtt
    13141 tacaactatt ctgtctagtt taagacttta ttgtcatagt ttagatctat tttgttcagt
    13201 ttaagacttt attgtccgcc cacaagccct tttttatcta tgatagtgat tactcaaccg
    13261 taaccgattt tgccaggtta cgcggctggt caacgtcggt gcctttgatc agcgcgacat
    13321 ggtaagccag cagctgcagc ggaacggtgt agaagatcgg tgcaatcacc tcttccacat
    13381 gcggcatctc gatgatgtgc atgttatcgc tacttacaaa acccgcatcc tgatcggcga
    13441 agacatacaa ttggccgcca cgcgcgcgaa cttcttcaat gttggatttc agtttttcca
    13501 gcaattcgtt gttcggtgca acgacgataa ccggcatatc ggcatcaata agcgccagcg
    13561 gaccgtgttt cagttcacca gcagcgtagg cttcagcgtg aatgtaggag atctctttca
    13621 gcttcaatgc gccttccagt gcgattgggt actgatcgcc acggcccagg aacagtgcgt
    13681 gatgtttgtc agagaaatct tctgccagag cttcaatgcg tttgtcctga gatagcatct
    13741 gctcaatacg gctcggcagc gcctgcagac catgcacaat gtcatgttca atggaggcat
    13801 ccagaccttt caggcgagac agcttcgcca ccagcatcaa cagcacagtt aactgagtgg
    13861 tgaatgcttt agtggacgcc acgccgattt ccgtacccgc gttggtcatc agcgccagat
    13921 cggattcgcg caccagagaa gaacccggaa cgttacagat tgccagcgaa ccaaggtaac
    13981 ccagctcttt cgacagacgc aggcccgcca gggtatccgc ggtttcgcca gactgtgaca
    14041 aggtgatcat caggctgtta cgacgcacgg cagatttacg atagcggaat tcagaggcga
    14101 tttcgacgtc gcacggaata cctgccagcg attcaaacca gtagcgggaa accataccgg
    14161 agttataaga agtaccacag gcgaggatct gaatatgctc aaccttcgac agcagttcgt
    14221 cggcgttcgg tcccagctcg cttaaatcaa cctgaccgtg gctgatgcgc ccggtaaggg
    14281 tgttttttat cgcgttcggc tgttcgtaga tctctttctg catgtagtga cggtaaatac
    14341 ctttatcgcc cgcgtcatat tgcagattgg attcgatatc ctgacgtttt acttccgcgc
    14401 cagttttatc gaagatgttt accgaacggc gagtgatttc cgcaatatcg ccctcttcaa
    14461 ggaagataaa gcgacgggtc accggcaaca gcgccagctg gtcagaagcg ataaagtttt
    14521 cgcccatacc caggccaatc accagcggac taccagaacg tgccgccagc agggtatccg
    14581 ggtgacggga gtccatgatc actgtaccgt acgcaccgcg cagctgcggg atagcacgca
    14641 gaacagcctc acgcagagtc ccgccttgtt tcagctccca gttcaccaga tgggcaatca
    14701 cttcggtgtc ggtttcagaa acgaaggtat agccacgcgc ttttaactct tcacgcagcg
    14761 gttcatggtt ttcgatgatg ccgttatgca ccaccacaat gtgttcagaa acatgcggat
    14821 gcgcgttagc ttctgaaggt tcaccgtggg tcgcccagcg agtgtgagca ataccggtgc
    14881 cgccatccag aggatgttct tccgctgcct gagccagcat ctggacttta ccgaggcgac
    14941 gcaggcgggt catatgacct tccgcatcaa caacggccag accggcagag tcatatccgc
    15001 ggtattccag acgacgtaaa ccttcaagaa ggatttctgc tacatcacgt tgcgcgatcg
    15061 cgccaacaat tccacacata gtttttgatt ccgatttata tcgttgtcgg tcaacctgta
    15121 tgcccgtttt ttcgggcgcc ccgagccttg tagagagtgg ggttattttt atagttactg
    15181 cttgtgggag ggagattatg ttatctcctc atcccagatg accggattac ccggccagaa
    15241 ttactttttc tttaccggac gacgccagcc ttctttctga gtctgcggca cacggctgat
    15301 agctaatgca ttttcgccga cattacgcgt cacagttgta cccgcagcaa tggtcgcgcc
    15361 tttgcctact gttaccgggg ccaccagctg agtgtcggaa ccgacaaaca catcgtcgcc
    15421 gataatggtc ttaaatttat tcgcaccatc gtagttgcag gtaatggttc ccgcgccgat
    15481 gttaacgtta tcgccaattt ccgcatcgcc caggtaagtc agatgcccga ctttcgagcc
    15541 tttacccaga cgcgcttttt tcatctcaac gaagttacca acgtgtgcac cttccagcaa
    15601 ctcagcacca ggacgcaaac gagcaaacgg gccaatagta caggccgctg ccagattcgc
    15661 gtcttccaca acggtatacg gactgatttc gcaatcatcg ccaatcacgc tgtttttaat
    15721 cacgcaaccg gtgccgattt tcacgcgatg accgagagtc acgttgccct cgatgataac
    15781 gttagtatca atttcaacat cgcgcccgtg agtaagcgta ccgcgcagat caaaacgcgc
    15841 cggatcgcgc agcataacgc ctgctaacag cagtttttca gcctgttcgg actggtaaac
    15901 gcgctccaga cgggagagtt gcaggcggtt attcacacct tctacttcgc ttaaacgttg
    15961 cggatgaacg gcgacgattt cacgcccttc ctgatacgcc agcgcaataa tgtcggtgat
    16021 gtagtattcg ccctgagcat tattgttggt cagcttcgcc agccagcgtt tcatatctgc
    16081 gccgttagcg atcagaatgc cggtgttgat ctcctgaatc tgacgctgct cgtcggtggc
    16141 atctttgtgc tcaacaatgc cggtaacttt gccattttca cgggtgatac gtccataacc
    16201 ggtcggatca tccagtttca ccgtcagcag accaatgcca ccctgcggtt tagcatcacg
    16261 caggcgctgg agtgtttcga cagagatcag cggcacgtcg ccgtagagca ttaaaatgtc
    16321 ttcatcatcg gcaaagaaag gtgcggcctg ctgcattgca tgacccgtac ccagctgctc
    16381 tgcctgaagc acccagttaa ggttgtcgtc tttcagcgcc tgtttaagca gatcgccgcc
    16441 gtgaccgtac accaagtgaa cgtgcgctgc gcctaattca ttcgcagcat caatgacatg
    16501 ctgaaccatc gctttcccgg caagggtatg cagcactttc ggaagatcgg aatacatgcg
    16561 cgtgcctttg cctgcggcaa ggatcactac gctcatagca ttattcaaca tacgcgtcct
    16621 gactgtaatt tgagaacgaa tttaaaccgc ttcaccttga aaaaactaca tttttttcat
    16681 cgtgaaatgg acagaggata aattgttcaa taacggatta tccccgctga cgaaagtgcc
    16741 attttcgacc atcgtcacgc cttttgtctg cagaaaatta aggcagtacc ttcatttaag
    16801 cgtcaggtgg atgtttttgc tcttatttcg atcaatgaat aaacgggaaa acaggggtgt
    16861 tttatatcag caggatctat gtgaacgctc ttcaggacgg atcacgcgta aaaaaaagcc
    16921 agcctgtttc cagactggct tttgtgcttt tcaagccggt gttacatcgc ttttttggtc
    16981 aactcgataa cgcgcagctg cgcgatcgct ttggccagtt ccgcagacgc ctgagcgtaa
    17041 tctacgtcgc cgtgagagct gctaatgtgc tcttcagcct tacgtttcgc ttccatggct
    17101 cgcgcttcgt cgagatcctg gccgcgaatt gcggtgtcgg ccagaacggt cacgttgcca
    17161 ggctgcactt caagaatgcc gccagacaga tagataaact cttcgtgacc gtgctgtttc
    17221 acgatgcgaa tcataccagg cttaatggcg gtgagcagcg gtgcgtggcc agggtagatc
    17281 cccagttcac cttcgctacc cgttacctgg attttctcga ccagaccaga gaacatttgt
    17341 tgctctgcgc tgacgacgtc caggtggtaa gtcattgcca tatcaccctc cgattaaggc
    17401 gttaaagttt tttggctttt tccacagctt cttcgatgga accgaccatg tagaacgcct
    17461 gctccggcag gtgatcgtat tcgccttcca tgatgccttt aaagccacgg atggtgtctt
    17521 tcagggagac gtatttaccc ggagaaccgg tgaatacttc tgccacgaag aacggctggg
    17581 acaggaagcg ctggatctta cgagcacgcg ctaccaccag tttgtcttct tcagacagtt
    17641 catccatacc caagatggcg atgatgtctt tcagttcctg ataacgttgc aggatggact
    17701 gaacgccacg cgcggtgtcg tagtgttcct gaccaaccac cagcgggtcc agctgacggc
    17761 tggtggagtc cagcgggtca acggccgggt agatacccag agacgcgatc tgacggctca
    17821 gtaccacggt tgcgtcaagg tgcgcaaagg tggttgccgg agacgggtca gtcaagtcat
    17881 ccgcaggtac gtataccgcc tgtacggagg tgatagaacc agttttggtg gaggtgatac
    17941 gttcctgcag aacgcccatc tcttccgcca gggtcggctg ataacctacc gctgaaggca
    18001 tacggcccag cagtgcggat acttccgtac cggccagggt gtaacgatag atgttgtcaa
    18061 cgaacagcag aacgtcacga ccttcgtcac ggaatttctc agccatggtc agaccggtca
    18121 gagcaacgcg cagacggttt cccggcggct cgttcatctg gccatacacc agggatactt
    18181 tgtcgataac gttggagtcg gtcatttcgt ggtagaagtc gttaccctca cgagtacgtt
    18241 cacctacgcc cgcaaacaca gagtaaccgg agtgctcgat cgcgatgtta cgaatgagct
    18301 ccatcatgtt tacggtttta cctacacccg caccaccgaa cagaccaact ttaccgccct
    18361 tagcgaacgg acacatcagg tcgataactt tgataccggt ttccagcagt tcctgagagt
    18421 ttgacagctc ttcgtaggaa ggtgctgcgc ggtgaatcgc ccaacgctct tcttcaccga
    18481 tctcgccttt catgtcgacc ggttcaccca gtacgttcat gatacggccc agagtcgctt
    18541 tacctaccgg gacttcgatc gggtgttcga ggtcttttac atccagaccg cgacgcagac
    18601 cgtcggagga acccattgcg atggtacgca cgataccgcc gccgagctgc tgctgaactt
    18661 ccagcaccag acgctcatta ccattttgca cctcaagagc atcgtacacg cgcggtacgg
    18721 catcctgagg gaattcgacg tcaactacgg cgccgattac ctggacaatc tttccagtag
    18781 ccatcttaaa tcctctacga aataacctgt ttaaaccgcg gcggcccccg agacgatctc
    18841 ggtgagttcc tgagtaatgc tggcctgacg agctttgttg tataccaact gcagctcttt
    18901 aatcaggctg ccgccattgt cggtcgcggc tttcatcgcc accatacggg cggcctgctc
    18961 gctggccagg ttttcaacca cgccctgata aacctgagat tcgacataac gacgcagcag
    19021 ggtatccagc aacgccttcg gatcgggttc gtacaggtaa tcccaggatt tatgtttcag
    19081 atcatcatca tctgatgccg gtaacggcag cagctggctg atggtcggaa cctgagacat
    19141 ggtgttaata aatttgttgc tgacaatgta aagcttgtcc agacggcctt cgtcgtaagc
    19201 ctgcaacatc acttttaccg gaccgatcag ttcggacagg gaagggttat cccccatgcc
    19261 ggtgacctgg gcaacaacat tgccgcccac ggagttgaag aacgacacgc ctttcgagcc
    19321 gatcattgcg aggtcgcatt gaacgctttt gtcggtccag gtcttcattt ccgccagtag
    19381 ttttttgaac aggttaatgt tcaaaccacc gcacaaacca cggtcggtcg acaccaccag
    19441 gtagcccacg cgtttaacgt cgcggtcttc caggtaaggg tgcttatatt ccagattacc
    19501 gtgtgcaagg tgaccaatca ctttgcgcat ggtttctgca taaggacggc tggccgccat
    19561 gcgatcctgc gatttacgca ttttggaagc ggcgaccatc tccatcgctt tagtgatctt
    19621 ttgcgtgttc tggacgcttg cgatcttact acgtatctct tttgcgccgg ccatgagctt
    19681 ctcctcaatg ccttgcggcc tgccctaagg caagccgcca gacgttacca ggattgggtt
    19741 gctttgaagg aatcgaggat gcctttcagc ttgccttcga tttcgtcgtt gtagccaccg
    19801 gtctggttga tctcttgcat caacggagcg tgatcacggt cgacgtaagc cagcagagcg
    19861 gcttcgaagc tgccaatttt cgacagttca acatccgcca ggtaaccacg ttctgctgcg
    19921 aacagaacca gagactgctg cgcaacggac atcggcgcat actgtttctg tttcagcagt
    19981 tcggtcactt tctgaccgtg gtcaagctgc ttacgtgttg catcgtcaag gtcggatgca
    20041 aactgagaga acgctgccag ttcacgatac tgtgccagag cggtacggat accaccggac
    20101 agttttttca tgatcttggt ctgtgctgca ccaccaacac gggatacgga aatacccggg
    20161 ttaaccgcag gacgaatacc ggcgttgaac aggttggttt ccaggaagat ctgaccatcg
    20221 gtaatggaga ttacgttggt cggaacgaac gcagaaacgt cacccgcctg agtttcgata
    20281 atcggcagtg cggtcagaga accggttttc cctttcactt cacctttggt gaaggcttca
    20341 acgtattcgg cgttaacacg tgcagcacgc tccagcagac gagagtggag gtagaaaacg
    20401 tcgcccggga atgcttcacg tcctggcgga cgacggagca gcagggagat ctgacggtaa
    20461 gcaacagcct gtttagacag gtcatcgtaa atgatcagcg catcttcacc gcggtcacgg
    20521 aagtattcgc ccattgcgca accggcatac ggtgccaggt attgcagtgc agcggattca
    20581 gacgcggttg ctaccacaac gatggtgtta gccagtgcgc cgtgctcttc cagtttacgt
    20641 accacgttag aaatggtgga cgctttctgg ccgatagcga catagataca tttgataccg
    20701 gaatcgcgct ggttgatgat ggcatcgata gccagtgcgg ttttacctgt ctgacggtca
    20761 ccgatgatca attcacgctg accacgaccg attgggatca tggagtcaac ggctttataa
    20821 ccggtctgta ccggctgatc tacggactga cgttcgataa cgcccggagc gattgcttct
    20881 acagcagaga agccgtcgtg atccagcgga cctttaccgt cgattggtgc acccagagtg
    20941 ttaaccacac ggcccagcag gccacggcca accggaactt ccaggatacg gccagtacac
    21001 ttaactttca tgccttcggc aaggtcagcg tacggaccca taacaaccgc acctacagag
    21061 tcgcgctcaa ggttcagtgc gatagcgtaa cggttacccg gcagggagat catttcaccc
    21121 tgcatacaat cggccaggcc gtgaatgcgg ataacaccgt cacttacaga aacaatagta
    21181 ccttcgttgt gagcttcact cacaacattg aactgagcaa tgcgctgctt gatcagttcg
    21241 ctgatttcgg tggaattcag ttgcatgctc cagtcccctt aagactgcaa gacgtctgca
    21301 aggcgctcaa gacgaccgcg tacgctgcca tcaatgacca tatcacccgc tcggatgata
    21361 acgcctgcca ttacagactt atcgattttg caattcagct taactttgcg tgacagacgt
    21421 ttttccatcg cagcagaaat tttcgcgagc tgttgttcac tcagtgcggc agcggaaatg
    21481 acgtctacct cagcggtagc ctcactcacg gcacgaaggt gaataaactg ctccagaaca
    21541 tccgggagcg cgttaagacg accattttca gccattaccc gaatcaggtt ctgaccgttt
    21601 tcgtccagtt gctcaccaca aactgcgata aacgactcgg cgagcgtttc tggcgcaagc
    21661 gcgccagaga gaagctctgc catttgttcg tttttggtta cctcggcggc aaacgccagc
    21721 atgtcctgcc agcgttctac actttggtgt tcgacggcaa agtcaaaagc tgctttggcg
    21781 taggggcgag ctaccgtaat aaattcagac atcagcccct ccctccttac agttcagcga
    21841 caagtttatc cacgatgtcg ctgttagcag cttcatccac ggaacgttcg atgatcttct
    21901 cggcgccagc aacagccagg atagcaactt gcttacgcag ctcttcacgg gcacgtttac
    21961 gctcggcttc aatttctgcc tgcgcctggg ccacgatttt agtacgttcc tgttctgcct
    22021 cagctttcgc ttcgtccaga atctgcgagc ggcgtttgtt cgcctgctcg atgattacct
    22081 gggcttccgc tttcgctttt ttcagctggt cggtcgcgct ggcctttgca aggtcaaggt
    22141 ccttatgtgc tcgttctgcg gaagcaaggc cgtcagcaat ttctttttga cgtttttcga
    22201 tggctgccat taatggcggc catacgtact tcatgcagaa cagaacgaac aggacaaacg
    22261 cgatggcctg gccgaggatt gttgcgttaa gattcacagc acaatgcctc tatttagtta
    22321 acgttctgat attgctcttt aaataaaagc aacgcttact acgcgacagc gaacatcacg
    22381 tacagaccca gacctacagc gatcatcggg atagcatcca ccagacccat aacgataaag
    22441 aactgagtac gcagcagagg aatcagatca ggttgacgcg ctgcgccttc caggaattta
    22501 cccccgagga tgccgatacc gatcgcagca ccgattgccg ccagacccat catcacagcg
    22561 gcagccatgt acagcagatc catattcagg ttttccatga cagtctccag tttgtttcag
    22621 ttaaaacgta gtagtgttgg taaattaatg ttcttcagac gccatcgaca gatagacgat
    22681 cgtcagaacc atgaagatga aggcttgcag cgtaatgatc aggatgtgga aaatggccca
    22741 cggcacattc aggatccact gtgaccacca cggcaacaga ccagcaatca gaatgaaaat
    22801 cagctcaccg gcatacatgt taccgaacag tcgcaaaccg agtgaaactg gtttggacag
    22861 caggcttacc ccttcaagga ttaagttgac aggaatgaac gcccagtgat tgaacggctg
    22921 cagcgtcaac tctttcgtga agccgccgat gcctttcatt ttgatgctgt agaacagaat
    22981 caggataaat acgcccagtg ccatagacag cgttacgttc acgtccgcag acggaaccac
    23041 acgcagtgca ggcagaccca gtacatgttc agcaatgtac ggcagcaggt cgataggcag
    23101 taaatccatc aggttcatca ggaataccca gacgaagatc gtcagggcca gcggagcaat
    23161 cagcttgctt ttgccatggt acatgtcttt cacgctacca ttaacaaagc cgatcaccag
    23221 ctcaatcgcg gtctgaaact tacctggcac accgctggtc gcctttttgg ctacgctacg
    23281 gaataaaacc aggaacaaca gacccagcac caccgagaag aacatggagt caatattgat
    23341 tgtccagaag gtggctgggg ggttttgtgg atccaccagc gagaatgtac gcaggtccag
    23401 ctgaaggtta ttcaggtggt gtcctatgta atcctgcggc gtcatatttt ctgaagccat
    23461 gatgcctttt accctttgtt gttaattaca gccggtgcca gtatctgaac caccagcacc
    23521 aaaacccacg taacgatcag cggcaagaat accgccttta aaaccgccaa cgccaccacc
    23581 agtaacacca acatcgccag aactttgaaa gcttcgccaa atgcgaatgt ccaggccacc
    23641 cggcctttcg ctggtgtata cgcctggtga cgccaggcaa atatcataaa caaaacgtta
    23701 ggcagaaaga ctgccaggcc cccgcttatt gcagagacgc cccagaaggg gtctttgagg
    23761 ctgaacagca atccacttgc tatcaccacc agtaactgaa cgagcagaag cttccgagca
    23821 acgtttcgac tcacgagcga cacagacatc acgtttttca ctcctgctcc cttcgaggta
    23881 tgccgcgtgt cgtataaaac tttctttaag gcttagagtc aagcatcaaa aagcggtcaa
    23941 attatacggt gcgcccccgt gatttcaaac aataagtagc caaaaggtga ataaatgttt
    24001 aaatattttt ccagtgcata caattgcgac ttttccgcta accctgttcg atcatgaaaa
    24061 actgtaaata acgcgtaaac actagtgata aagcgtgctt cagatcacat attgcgcatg
    24121 ttcgcgcaca gtatatttat ttacttggca aatgatgcct ttgcaagttt atgatatttc
    24181 agtctaaaaa cagatactgt tttaataaat gacatttaca caacaaaaac cacccattga
    24241 catttttaat aatgttttaa cagccaatga tggttcttag cgccgatttt tagcagactg
    24301 atattttcac taatgactta ttttctgctt accaaaaaaa gccacgttat cttgttgata
    24361 caaaagagtg aacgtggcgt taaatgtaac cagttatatc agtagaaaac ttggttgttg
    24421 ttaacagtct aaccggtcaa ttttttatga tttttttgat aaaaattaaa ttttatttgc
    24481 tttaatcacc accagatgac gttcgccatc cagagctgga acctgaagtt taaccactga
    24541 ttcgacctga tattcttcgg gcaacaaagc gatttcatct tccggcattt gccctttcag
    24601 cgcgtagaaa cggccttgct caccaggaag atggtggcac cagctcacca tatcgttcag
    24661 agaggcaaaa gcgcggctaa ttacgccatc aaatggcggc tctgaaggaa actcttctac
    24721 cctgctctgt actggctcaa tattctccag tttaagctca tgttgcacct gacgaaggaa
    24781 acgcacgcgt ttaccaaggc tatccaacag agtgaaatgg gcttcaggac gcacgataga
    24841 gagtggaatg cctggcagtc ctggtccggt gccgacatcg ataaaccgtt caccttgcag
    24901 atacggtgcc accacaatgc tatcgagaat atggcgtacc agcatctcat taggatcgcg
    24961 gaccgaagtc aggttgtacg ctttgttcca tttatgcagc atattcacgt aggcaataag
    25021 ctggtttttc tggtgatcgg taagcgaaat acctgcgtct ttcagcagta aggagagttt
    25081 gttgagcacg gtgattacct gttcttgatg cgttgcctgg taagcgggtg cttaccaggc
    25141 atttttaatg cgttatgcgc tacgacgcag cataccctgt tttttcagcc acaccagcag
    25201 aatggagata gccgcaggcg tgacgccaga aatacgcgaa gcctggccga tagaggccgg
    25261 tttgtgatcg ttaagtttgg cgatcacttc gttagaaaga ccggatacct ggcggtaatc
    25321 cagtgtcgcg ggtagcaggg tgttctcgtt acgcagctgc ttttcgatct catcttgctg
    25381 gcgcgcgata taaccttcgt atttaacctg aatctcaacc tgttccgccg cctgttcgtc
    25441 tgtcaacgca ggggcaaacg gcgtcagcgt ggttaatttt tcataagtca tttccggacg
    25501 acgcagcaga tcttcaccac tggcttcacg ggaaagcggc gcagtcaggt gagcattcac
    25561 ttcggctgca gcttccgccg acggggttac ccaggtcgat ttcagacgct gacgctcacg
    25621 ctcgatattc tcaagtttct cgttaaagcg cgcccaacgt tcgtcatcca ccaggcccag
    25681 ttcacgaccg atttcagtca aacgcagatc cgcattatct tcgcgcagca tcagacgata
    25741 ttctgcgcgc gaagtaaaca tacgatacgg ttctttggtt cctaaagtgc acaggtcatc
    25801 aaccagtacg ccgagatacg cctgagaacg cgccggagcc caaccttctt tgtcagcaga
    25861 cagacgggca gcgttaagac cgaccagcaa accttgcgca gcggcttctt cgtaaccggt
    25921 agtgccgtta atctgaccag caaagaacag cccctggata aacttgctct ccagcgtcgg
    25981 tttcaggtcg cgtggatcga agaagtcata ctcaatggca taacccggac gcacgatctt
    26041 cgcgttttcc atcccctgca tggagcggac gatttgcatc tgcacatcga acggcaggct
    26101 ggtggagata ccgttcggat aaatttcgtt agaggtcagc ccttccggtt caaggaagat
    26161 ctgatgctga tttctgtcgg cgaagcgcat gactttgtct tcgatcgatg ggcagtagcg
    26221 tgggccgaca ccttcgatca cccctgcgta cattgggcta cgatcgaggt tactgcggat
    26281 cacatcatgg gttttctcgt tggtatgagt gatataacac ggcacctgct ggggatgctg
    26341 ggacgcattg cccataaacg agaataccgg cattgggtta tcgccatgct gttgcgccag
    26401 cacactaaag tcgatagttc gagcatcaat acgcggtggt gtcccggttt tcagacgacc
    26461 aacgcgcagc ggcagttcac gcaaacggcg agaaagcgga atggacggcg gatcaccagc
    26521 acgaccaccg ctgtaattat ccagaccgat atgaatttta ccgtcgagga acgtcccaac
    26581 ggtgagcacg acggctttgg cacggaactt cagtcccatt tgggtaacag caccgaccac
    26641 gcgatcgttt tcgacaataa gatcttcaac cgcctgctgg aagatcatca ggttcggttg
    26701 gttctccagc gccgtacgta ccgcctgacg gtagagcaca cgatccgcct gagctcgggt
    26761 agcgcgaacc gccggtcctt tgcttgcgtt tagtatccta aactggatac ccgcttgatc
    26821 gatcgctttc gccatcagac cgccgagtgc atccacttct tttaccagat gtcccttccc
    26881 aataccgccg atcgccgggt tgcagctcat ctgccccaga gtgtcgatat tgtgtgtcaa
    26941 aagcagagtc tgttgaccca tacgcgccgc ggccatcgcg gcctcggtgc ctgcatgacc
    27001 cccgccaatg atgatgacgt caaaaggatc cggataaaac atggtgattg cctcgcataa
    27061 cgcggtatga aaatggattg aagcccgggc cgtggattct actcaacttt gtcggcttga
    27121 gaaagacctg ggatcctggg tattaaaaag aagatctatt tatttagaga tctgttctat
    27181 tgtgatctct tattaggatc gcactaccct gtggataaca aggatccggc ttttaagatc
    27241 aacaacctgg aaaggatcat taactgtgaa tgatcggtga tcctggaccg tataagctgg
    27301 gatcagaatg aggggttata cacaactcaa aaactgaaca accgttgttc cttggataac
    27361 taccggttga tccaagcttc ctgacagagt tatccacagt agatcgcacg atctgtatac
    27421 ttatttgagt aaattaatcc acgatcccag ccattcttct gccggatctt ccggaatgtc
    27481 gtgatcaaga atgttgatct tcagtgtttc gcctgtctgt tttgcaccgg aatttttgag
    27541 ttctgcctcg agtttatcga tagccccaca aaaggtgtca tattcacgac taccaatacc
    27601 gattgcgcca aagcggaccg cagaaagatc gggcttctgt tcctgcaatg cttcatagaa
    27661 aggagaaagg ttgtccggaa tatctccggc accgtgggtg gagctgataa tcagccagat
    27721 ccccgaggca gataaatctt ccaacagcgg accgtgcagc gtttcggtgg taaaacccgc
    27781 ctcttccagc ttttcagcca ggtgttctgc tacatattcg gcaccgccga gggtgctgcc
    27841 gctgataaga gtgatatctg ccataaaccg ccacctttat taagagtggc gtattgtacg
    27901 ctgtgaacgc gttgggatct acctgtggaa aagtatggga ttaaaaaagc cgattatggc
    27961 ttgatggtac gcatgatcgg gttttgcagg acgatcaatg tctcggtgga ctgaatttca
    28021 tcaattgttt ggatcttgtt gataagtaca tgctggagag cgtcgatcga acggcacatt
    28081 acttttataa agatgctgta gtggcctgtt gtgtaatagg cttcagtgac ttcagcaagg
    28141 ctttccagct ttgccagcgc ggaagggtag tctttggcgc tctttaatat aatgccgata
    28201 aagcagccta cgtcataacc gagctgcttc gggctgacat caatacgcgc cccggtaatg
    28261 atccccgcct gcttcatttt ctctactcga acgtgaatcg tccccggact gacgccaaat
    28321 tgtttcgcca gttcggcgta agcggtgcgc gcattgccca ttaatgcttc caggatgcca
    28381 cggtccagat tgtcgatcag ataattttcc ataggatttt cttatgcaga ttgatgattc
    28441 attctatttt agccttcttt tttaatgaat caaaagtgtg ttaggctttt tattgaatga
    28501 ttattgcatg tttgtcggtt tttgttgctt aatcataagc aacaggacgc aggagtataa
    28561 aaaatgaaaa ccgcttacat tgccaaacaa cgccaaatta gcttcgtgaa atctcacttt
    28621 tctcgccaac tggaagaacg tctggggctg atcgaagtcc aggcgccgat tcttagccgt
    28681 gtgggggatg gcacgcagga taacttgtcg ggctgtgaaa aagcggtgca ggtaaaagtg
    28741 aaagctctgc ctgatgccca gttcgaagtg gttcattcac tggcgaagtg gaaacgtcag
    28801 accttagggc aacacgactt cagcgcgggc gaagggctgt acacgcacat gaaagccctt
    28861 cgccccgatg aagaccgtct ttctccgttg cactcggtct atgttgacca gtgggactgg
    28921 gaacgcgtaa tgggcgacgg tgagcgtcaa ttctcgactc tgaaaagcac ggtagaggcg
    28981 atctgggcgg gaattaaagc aaccgaagct gcggttagcg aagagtttgg cctggcaccg
    29041 ttcctgccgg atcagatcca cttcgtacac agccaggagt tactgtctcg ttatccggat
    29101 ctcgatgcca aagggcgtga gcgggcgata gcgaaagatc ttggcgcggt attccttgtc
    29161 gggattggcg gcaagctgag cgatggtcat cgccacgacg tgcgcgcacc ggattatgat
    29221 gactggagca ccccgtcaga gctgggtcat gcgggtctga acggcgatat tctggtgtgg
    29281 aaccctgtac tggaagatgc gtttgagctt tcttccatgg ggatccgcgt ggatgccgac
    29341 acgttgaagc atcagttggc gctgaccggt gatgaagatc gtctgcagct ggaatggcat
    29401 caggcgctgc tgcgcggtga aatgccgcag accatcggcg gcggtatcgg ccagtctcgt
    29461 ttgaccatgc tgctgctgca actgccgcat atcggccagg ttcagtgtgg tgtatggcca
    29521 gctgctgttc gcgagaacgt cccttctctg ctgtaataat ttatcgccgc cagcgtctga
    29581 gcaggcggct tcgcatcccg gtatcaaagc gccagatatg atcgaaaatg cgcatgatgc
    29641 cgggtttgcc gtgtgccgac atcgccacgg catgaaagcg atgctgatgt acccgctgca
    29701 gctctttcac tttactcgtc acgtcgtcag gcaaccgctg agcgataaaa tcagaaatca
    29761 ccaccgcatc ggcatcaaac cattccctgc tttgcaagcg ttccataatg gcgcgaaaac
    29821 aactggcaag atcggtgccg ccacgaaact gctggcttaa aaaacggatt gcttgttcga
    29881 tgccttgggg gcctgaaagc tcataacgga cgatctcggt ggaaaatagc ataatatagc
    29941 agcgccggtt ttcagcgaga gcaatgcgca tcaaggccag gcagaacgct ttcgcacact
    30001 gttcattaaa gccgcccatt gagccggaag tatccacaca gacaataaac ggcccgcgcg
    30061 gctgttcatc gtaatcttta tgtaccaccg gacgttcgat cactttttca cgccacgact
    30121 caccgtgcag gcgataggtg agcaactgtt tttccaccag ccgacggtaa aactcatact
    30181 ccagttccgt tatccctagt gtcgccagtt ctggcggcag aagacgtaaa atatcatcgc
    30241 tttgttgcag accatcaacc tgctcaggaa ccgtcgccgg ttcgcgcacc atggtgcgga
    30301 aggtttccat ctgcgcatcg ttgcgcggta ttgatttggc ttcccgagaa cgccccagct
    30361 gctctgccag gcgtttcagt tccggctgtt cgttaagaaa ttcaccgtat ttcacaatca
    30421 actgatagtc gccacgttta agctgaccgg cgctcatatc ccacagacga ccagctgcag
    30481 tattgttatc tgcgagaatc ggttcaagtt gtccgctcag cgtcatgcgt tcttgaactt
    30541 cactcaacag ttgttcgcgt tcttcttcta atagctgttg atttaacgtc gttgcttgca
    30601 cgatcagact taaacgccaa cgctggagaa aaagcgtgtg taacgctgaa gttattgtgc
    30661 tgttagcatc aaccaactgc cgggcttgtt ctgcccatgg ggaattcaga cgatgcagta
    30721 agtccaggat ctgtggtagc tgcacaataa actgcggcgt ggagaggagc tggctttgct
    30781 gatagcacat cacctcttcg gtgagttccg gcgggactcg ggcatctttc agccgactgc
    30841 gcagcgcctc acgccagcgg ggaacatcat cagtgattgc cgccttcagg cgtgggaatt
    30901 tttcaaagaa gaccgccagc tgcggtgagg ccagcagtgc gatgatcatc tcttcgatca
    30961 atccctcttc gctgacggcc agcatcacat taagcgtatc cagcgttagc attgttgtgc
    31021 ctggtgaatc tgtgcaccga catcctgtag gctggcttca atgcgaccta accagtcgcc
    31081 agggataaac agacattttt gctgttcgct gaataaagcg tgttgcttgc gccagtcgct
    31141 ttcaagctct tccagttgtt gttttatttc accaggcaga ccttcagccg atgaaccggg
    31201 aagtgccagc gtactgcctt gtaaacttac atcgcgaacg acaagatgtt gggcgctatc
    31261 aacttccaga ttcagtttct gggcaaagcc gataccgttc agtttaccgc gaatttcacc
    31321 acctttgctc agccactgtt ccagcgcgct acgctcaaag gagatatgaa ccacttccat
    31381 atcatgcagt tttaacggtt tttgcagcag tagagtcaga gtggaagcag taacgttaac
    31441 aggaagttga tactgctgac gacggctgaa aatgccgccc agacgaatta ccgttaaggc
    31501 tgttttatcg ctttgttgct gctggagttg caggtgacgt tgcacaatcg cgccaaggcg
    31561 agtcaacatc ccttgctgtt gccaggcatg accggtcatc aatacatcaa tttgttgttg
    31621 tatcaaattc aggctttgcg cgtcatacca caggcaatct ttcagcaaaa tgagatctac
    31681 cggggcaaca gcactgcgac cgctaaaaaa ggcgctggcc tgcaataatc ggatcgcttt
    31741 tttccagcga cgatccgaga cataaggcgc atccggtaat ttatccagtt gcaggcgcag
    31801 cataaaaatc agctcaaata catgatcggg cagcgtaatt tcaccaatct ctttctgcca
    31861 gcgttcatat tcttcatctg tgacctgcaa ggcatcagga accggattgt cgttttcatc
    31921 ctgttgactg gtcagcatgg agcggaaatt cgctttatcc tgcactttat ctaaccacag
    31981 acgaatcagc atgcggtcat ataacgcttc cagactgctg tctgcttccg gcagctcgtt
    32041 ggaggccgcc accagcagac gcatcgggat tttttctacg tgtgcaccgt tgcggaactg
    32101 gcgctcgtta atggcggtga gcaaggtatt aagaattgcc gggcccgctt tccagatctc
    32161 atccagaaag acaatttctg cttccggcag gtaaccgctg gttaaacgtt catagcgccc
    32221 ttcatctttt agcgcctgaa tagaaagggg accaaaaact tcttccggcg tggagaagcg
    32281 ggtcatcaga tattcaaacg cgcgggcatt ctgaaaggcg aattttaagc gccgggcgat
    32341 caaacttttg gcaatacctg gcgggccaag gaggaacaca ctttcaccac ttaatgccgc
    32401 taataaacac aagcggatgg cgtggctacg ttcataaagc cccttttcca acgaactgct
    32461 caggcgggaa attctttccg ctaataaatg agggtgagcc ataatgaagt ggcgtccttt
    32521 cgtcaaaagt tctgcgtaaa ttgcgagtat agacgtttct tgctggtggc taaaatagtc
    32581 tcaaag
//
LOCUS       DAETQN010000039        32532 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.039, whole genome shotgun sequence.
ACCESSION   DAETQN010000039 DAETQN010000000
VERSION     DAETQN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.039"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..94
                     /locus_tag="K0W68_001800"
     CDS             <1..94
                     /locus_tag="K0W68_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3073378.1"
                     /translation="SLLAKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(136..1212)
                     /gene="glpQ"
                     /locus_tag="K0W68_001801"
     CDS             complement(136..1212)
                     /gene="glpQ"
                     /locus_tag="K0W68_001801"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBI3073379.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1217..2575)
                     /gene="glpT"
                     /locus_tag="K0W68_001802"
     CDS             complement(1217..2575)
                     /gene="glpT"
                     /locus_tag="K0W68_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBI3073380.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2848..4476
                     /gene="glpA"
                     /locus_tag="K0W68_001803"
     CDS             2848..4476
                     /gene="glpA"
                     /locus_tag="K0W68_001803"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBI3073381.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4466..5725
                     /gene="glpB"
                     /locus_tag="K0W68_001804"
     CDS             4466..5725
                     /gene="glpB"
                     /locus_tag="K0W68_001804"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBI3073382.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5722..6912
                     /gene="glpC"
                     /locus_tag="K0W68_001805"
     CDS             5722..6912
                     /gene="glpC"
                     /locus_tag="K0W68_001805"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBI3073383.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7105..8007
                     /locus_tag="K0W68_001806"
     CDS             7105..8007
                     /locus_tag="K0W68_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBI3073384.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8048..8851)
                     /gene="yfaU"
                     /locus_tag="K0W68_001807"
     CDS             complement(8048..8851)
                     /gene="yfaU"
                     /locus_tag="K0W68_001807"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HBI3073385.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8869..10158)
                     /locus_tag="K0W68_001808"
     CDS             complement(8869..10158)
                     /locus_tag="K0W68_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3073386.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10215..11419)
                     /gene="rhmD"
                     /locus_tag="K0W68_001809"
                     /pseudo
     CDS             complement(10215..11419)
                     /gene="rhmD"
                     /locus_tag="K0W68_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(11434..12216)
                     /locus_tag="K0W68_001810"
     CDS             complement(11434..12216)
                     /locus_tag="K0W68_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBI3073387.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12436..13638)
                     /locus_tag="K0W68_001811"
     CDS             complement(12436..13638)
                     /locus_tag="K0W68_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBI3073388.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(13738..14280)
                     /locus_tag="K0W68_001812"
     CDS             complement(13738..14280)
                     /locus_tag="K0W68_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073389.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14559..14984
                     /gene="nudI"
                     /locus_tag="K0W68_001813"
     CDS             14559..14984
                     /gene="nudI"
                     /locus_tag="K0W68_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBI3073390.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15023..15625)
                     /locus_tag="K0W68_001814"
     CDS             complement(15023..15625)
                     /locus_tag="K0W68_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBI3073391.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            15933..17072
                     /gene="arnB"
                     /locus_tag="K0W68_001815"
     CDS             15933..17072
                     /gene="arnB"
                     /locus_tag="K0W68_001815"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBI3073392.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17076..18044
                     /gene="arnC"
                     /locus_tag="K0W68_001816"
     CDS             17076..18044
                     /gene="arnC"
                     /locus_tag="K0W68_001816"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBI3073393.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18044..20026
                     /gene="arnA"
                     /locus_tag="K0W68_001817"
     CDS             18044..20026
                     /gene="arnA"
                     /locus_tag="K0W68_001817"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HBI3073394.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20023..20913
                     /locus_tag="K0W68_001818"
     CDS             20023..20913
                     /locus_tag="K0W68_001818"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBI3073395.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            20913..22565
                     /gene="arnT"
                     /locus_tag="K0W68_001819"
     CDS             20913..22565
                     /gene="arnT"
                     /locus_tag="K0W68_001819"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBI3073396.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22562..22897
                     /gene="arnE"
                     /locus_tag="K0W68_001820"
     CDS             22562..22897
                     /gene="arnE"
                     /locus_tag="K0W68_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBI3073397.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            22897..23283
                     /gene="arnF"
                     /locus_tag="K0W68_001821"
     CDS             22897..23283
                     /gene="arnF"
                     /locus_tag="K0W68_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBI3073398.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23277..23543)
                     /gene="pmrD"
                     /locus_tag="K0W68_001822"
     CDS             complement(23277..23543)
                     /gene="pmrD"
                     /locus_tag="K0W68_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBI3073399.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(23587..25008)
                     /gene="menE"
                     /locus_tag="K0W68_001823"
     CDS             complement(23587..25008)
                     /gene="menE"
                     /locus_tag="K0W68_001823"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBI3073400.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(25005..25967)
                     /gene="menC"
                     /locus_tag="K0W68_001824"
     CDS             complement(25005..25967)
                     /gene="menC"
                     /locus_tag="K0W68_001824"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBI3073401.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAEMADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(25967..26824)
                     /gene="menB"
                     /locus_tag="K0W68_001825"
     CDS             complement(25967..26824)
                     /gene="menB"
                     /locus_tag="K0W68_001825"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBI3073402.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(26839..27597)
                     /gene="menH"
                     /locus_tag="K0W68_001826"
     CDS             complement(26839..27597)
                     /gene="menH"
                     /locus_tag="K0W68_001826"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBI3073403.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27594..29264)
                     /gene="menD"
                     /locus_tag="K0W68_001827"
     CDS             complement(27594..29264)
                     /gene="menD"
                     /locus_tag="K0W68_001827"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBI3073404.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29353..30648)
                     /gene="menF"
                     /locus_tag="K0W68_001828"
     CDS             complement(29353..30648)
                     /gene="menF"
                     /locus_tag="K0W68_001828"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBI3073405.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30727..31032)
                     /gene="elaB"
                     /locus_tag="K0W68_001829"
     CDS             complement(30727..31032)
                     /gene="elaB"
                     /locus_tag="K0W68_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBI3073406.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31087..31548)
                     /locus_tag="K0W68_001830"
                     /pseudo
     CDS             complement(31087..31548)
                     /locus_tag="K0W68_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            31613..32530
                     /gene="rbn"
                     /locus_tag="K0W68_001831"
     CDS             31613..32530
                     /gene="rbn"
                     /locus_tag="K0W68_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBI3073407.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         7896 a         8805 c         8138 g         7693 t
ORIGIN      
        1 ttcgttgttg gctaaagaaa agccggtaga aggcagtcca ctgtttttac tacaaatgtg
       61 catggaacaa ttagtggcga ttggaaaaat ttgatatgag acaggatggc gcatgaacgc
      121 catcctgcag tagcattact ctttattaag gaattttact gccttatcag ggaaatcagt
      181 aaacagccca tttacacccg ctttgttata cagagcatca tataactgat tcacatcagt
      241 agtgtattca ggcagtttat ccgaccgcac ggtataagga tgcactacca gtttattctg
      301 ctgagcatct tgcaccatgc cagtgagttt gatattaccc ggctgcgatg tttcctcaat
      361 caacatatgg taatccggcc cgataccgtc ggcatattcc gcgacttgct tcatggcacc
      421 tggcttaaac atccagtcat agctgtaatt aacccagctt ccgtccggct gtttctgctg
      481 cgtttcattc cagtcggtat aggcaatcag ctgtaccaga ttgagatcca tgcccatttt
      541 aggttccagc tcattcttaa tacgcttcag ctcatcagca tcaaaacatt gcaaataaac
      601 tttatcgtcc ttaccggtgt aaccatattt cttcagcact tccagcgttt ttgccgcaat
      661 atccttccct tcctgatgat ggaaccacgg cgctttgatt tctggataga taccgatatt
      721 tttcccggta gagtgattta acccctgaac aaattcaatc tcttcttcaa aggtgtgccc
      781 ccggaagtcg gacttaccca ttgggaaacg ccccggataa gtctgtactt ttttaccgtt
      841 ttcaatatcg aaaccttcgg taaatttcag cgacttaatt tcatccagcg tgaaatctat
      901 cgcgtagtaa cgaccgtctt tgcgcgcccg atccgggaaa cgatcggcaa catcagtaac
      961 acgatcgaga taatggtcat gcagaacaac cagatggtcg tctttggtca tcaccaaatc
     1021 ctgttccaga taatccgctc cctgcgcata cgccatcgct tttgctggca gcgtatgctc
     1081 cggcaaatat ccactggcac cgcgatgggc gatgactatt ttttcgttgc tgtccgccgc
     1141 cattgcactg cttcccatga ctatagtgct catcatgatc gccatgctaa ggtttttcag
     1201 cttcaatttc atgccattag cctccgttgc gtttttgcag taattgttca tggcgacgtt
     1261 tttcgccaat catcacaaca atcaacaaga taaccgccag aatgctgccg ccaatcatta
     1321 ccataaagcc gccatcccag ccgaagaagt ccacggtgta gccaacaatc gcgctcgccg
     1381 ccaccgaacc gcccaggtaa ccaaacagcc cggtaaagcc cgctgccgta cctgccgctt
     1441 ttttcggtgc cagttccagc gcatgcagac cgatcagcat cacaggaccg tagatcagga
     1501 agccgataac aatcatacaa atcatatcga cggttgggtt acctgccggg ttcatccagt
     1561 aaacgatagt cgcgatggtc accagtgtca taaagaaaac gccggttgcc ccacggttgc
     1621 cacggaagac tttatccgac atccagccgc acagcagagt gcccggaata cctgcatatt
     1681 cataaaggaa gtaggcccag gaggatttat ccagcgcgaa atgcttaacc tctttcagat
     1741 aagtcggtga ccagtcgagg atgccgtaac gcagcagata aacgaacacg ttggcgatgg
     1801 cgatatacca cagcagtttg ttcggcagta cgtactgcat gaagatttgc ttcgccgtca
     1861 gctcctgttc cgctttttcg ttatagtcgt ccggataatc atttttgtac tcttcgatcg
     1921 gcggcaagcc acaggattgc ggggtatcgc gcatcatcgc aaaggcgaat aatgccacca
     1981 gaatggcgca gaaagcaggc atatagagcg ccgcatgcca gtcattgaac caggccatcc
     2041 ccagcaggaa cagcagcggc ggaataccac caccgacgtt gtgcgcacag ttccacactg
     2101 acacaatgcc gccacgttct ttctgcgacc accagtgcac catagtacga ccacacggcg
     2161 gccaccccat cccctggaac caaccgcaga ggaacaacag tacaaacatc accgcaatgc
     2221 tcgacgtcgc ccatggcaca aagcccataa acaacatcac tgccgccgcc agaatcaaac
     2281 ctgcgggcag gaaaacgcgc ggattcgagc gatccgatac cgaacccatg atgaatttcg
     2341 aaaatccata agcaatcgag atccccgaaa gggcaaaacc taaatcaccg cgtgagaatc
     2401 cctgctcaac cagataaggc atagcaagcg caaagttctt acgaaccaaa tagtaagccg
     2461 catagccaaa gaatatcccc aggaaaattt gccagcgcaa tcgacgataa gtcggatcga
     2521 tctccgcggc aggtaagcgc gctttgtgtg gcgctggttt aaaaatactc aacattgata
     2581 gcctccgtgg cccgtggtct tatttatgat taacagcctg attcagtgag agaacctgcc
     2641 gtttcttgag ttgccgcgat gttaagaaaa cattcataaa ttaaatgtga attgccgcac
     2701 acattattaa ataagattta caaaatgttc aaaatgacgc atgaaatcac gcttcacttt
     2761 cgaatcatga gcgaatatgc gcgaaatcaa acaattcatg tttttactat ggctaaatgg
     2821 taaaaaacga acttcagagg gataacaatg aaaactcgcg actcgcaatc aagtgacgtg
     2881 attatcattg gcggcggcgc aacgggagcc gggattgccc gcgactgtgc cctgcgcggg
     2941 ctgcgcgtga ttttggttga gcgccacgac atcgcaaccg gtgccaccgg gcgtaaccac
     3001 ggcctgctac acagcggtgc gcgctatgcg gtaaccgatg cggaatcggc ccgcgaatgc
     3061 attagtgaaa accagatcct gaaacgcatt gcacgtcact gcgttgaacc aaccaacggc
     3121 ctgtttatta ccctgccgga agatgacctc tccttccagg ccacttttat tcgcgcctgc
     3181 gaagaagcag ggatcagcgc agaagctata gacccgcagc aagcgcgcat tatcgaacct
     3241 gccgttaacc cggcattgat tggcgcggtg aaagttccgg atggcaccgt tgatccattt
     3301 cgtctgaccg cagcaaacat gctggatgcc aaagaacacg gtgccgttat ccttaccgct
     3361 catgaagtca cgggactgat tcgtgaaggc gcgacggtgt gcggtgttcg tgtacgtaac
     3421 catctcaccg gcgaaactca ggcccttcat gcgcctgtcg tggttaatgc cgccgggatc
     3481 tgggggcaac acattgccga atatgccgat ctgcgcattc gcatgttccc ggcgaaagga
     3541 tcgctgctga tcatggatca ccgcattaac cagcatgtga tcaaccgctg ccgtaaacct
     3601 tccgacgccg atattctggt gcctggcgat accatttcgc tgattggcac cacctcttta
     3661 cgtattgatt acaacgagat tgacgataat cgagtgacgg cagaagaggt tgatattctg
     3721 ctgcgtgaag gggaaaaact ggcccccgtg atggcgaaaa cgcgcatttt gcgggcctat
     3781 tctggcgtgc gcccgctggt tgccagcgat gacgacccga gcggacgtaa cgtcagccgt
     3841 ggcatcgtgc tgctcgacca tgctgaacgc gatggtctgg acggatttat caccatcacc
     3901 ggtggcaaac tgatgaccta tcggctgatg gctgaatggg ctaccgacgc cgtgtgccgc
     3961 aaactgggca acacgcgccc ctgtacgacc gccgatctgg cacttcctgg ttcacaggaa
     4021 cccgctgaag ttaccttgcg taaagtcatc tccctgcctg ccccgctgcg cggttctgcg
     4081 gtttatcgtc atggcgatcg cacgcctgcc tggctgagcg aaggtcgtct gcaccgtagc
     4141 ctggtatgtg agtgcgaagc ggtaacagcg ggtgaagtgc agtacgcggt agaaaattta
     4201 aacgttaata gcctgctgga tttacgccgt cgtacccgtg tggggatggg tacctgccag
     4261 ggcgaactct gcgcctgccg cgctgccgga ctgctgcaac gttttaacgt cacgacgtcc
     4321 gcgcaatcta tcgagcaact ttccaccttc cttaacgaac gctggaaagg cgtgcaaccc
     4381 atcgcctggg gagatgcact gcgcgaaagc gaatttaccc gctgggttta tcagggactg
     4441 tgtggtctgg agaaggagca gaaagatgcg ctttgatact gtcattatgg gcggcggcct
     4501 cgccggatta ctctgtggcc tgcaactgca aaaacacggc ctgcgctgtg ccattgtcac
     4561 gcgtggtcaa agcgcgctgc atttctcatc cggttcgctg gatttgctga gccatctgcc
     4621 tgatggtcaa ccggtgacag acattcacag tggactggaa tctttgcgtc agcaggcacc
     4681 agcccatcct tacagtcttc tcgaaccaca acgcgtgctc gatctcgctt gccaggcgca
     4741 ggcattgatc gctgaaagcg gtgcgcaatt gcagggcagc gtggaactgg cccatcagcg
     4801 cgttaccccg ctcggcactt tgcgtgcaac ctggttaagt tcgccagaag ttcccgtctg
     4861 gccgctaccc gcgaagaaga tatgtgtagt ggggataagc gggctgatgg atttccaggc
     4921 gcatcttgcg gcggcttcgt tgcgcgaact ggatctttcg gttgaaaccg cagaaataga
     4981 gctgccggaa ctggatgtcc tgcgcaataa cgccaccgaa tttcgcgcgg tgaatatcgc
     5041 ccgtttcctt gataatgaag aaaactggcc gctgttactt gatgcgctta ttcctgtcgc
     5101 caatacctgc gaaatgatcc tgatgcccgc ctgcttcggc ctggccgatg acaaactgtg
     5161 gcgttggttg aatgaaaaac taccttgttc actgatgctt ttgccaacgc tgccgccttc
     5221 cgtgctgggc attcgtctgc aaaaccagtt acagcgccag tttgtgcgcc agggtggcgt
     5281 gtggatgccg ggcgatgaag tgaaaaaagt gacctgtaaa aatggcgtag tgaatgaaat
     5341 ctggacccgc aatcacgccg atattccgct acgtccacgt ttcgcggttc tcgccagcgg
     5401 cagtttcttt agtggcggac tggtagcgga acgtaacggc attcgcgagc cgattctcgg
     5461 ccttgatgtg ctacaaaccg ccacgcgggg tgaatggtat aagggcgatt tttttgcgcc
     5521 gcaaccgtgg cagcagttcg gtgtaaccac tgatgagacg ctacgcccgt cgcaggcagg
     5581 gcaaaccatt gagaacctgt ttgccatcgg ttcggtgctg ggcggatttg atcccatcgc
     5641 ccaaggatgc ggcggcggtg tttgtgccgt cagtgcttta catgccgctc aacagattgc
     5701 ccaacgcgca ggaggccaac aatgaacgac accagcttcg aaaactgcat taagtgcacc
     5761 gtctgcacca ccgcctgccc ggtgagccgg gtgaatcccg gttatccagg gccaaaacaa
     5821 gccgggccgg atggcgagcg tctgcgtttg aaagatggcg cactgtatga cgaggcgctg
     5881 aaatattgca tcaactgcaa acgttgtgaa gtcgcctgcc cgtccgatgt gaagattggc
     5941 gatattatcc agcgcgcgcg ggcaaaatat gacaccacgc gcccgtcgct gcgtaatttt
     6001 gtgttgagtc ataccgacct gatgggtagc gtttccacgc cgttcgcacc aatcgtcaac
     6061 accgctacct cgctgaaacc ggtgcggcag ctgcttgatg cggcgttaaa aatcgatcat
     6121 cgtcgcacgc taccgaaata ctccttcggc acgttccgtc gctggtatcg cagcatagcg
     6181 gctcagcaag cacaatataa agaccaggtc gctttctttc acggctgctt cgttaactac
     6241 aaccatccgc agttaggtaa agatttaatt aaagtgctca acgcaatggg tatcggtgta
     6301 caactgctca gcaaagaaaa atgctgcggc gtaccgctaa tcgccaacgg ctttaccgat
     6361 aaagcacgca aacaggcaat tacgaatgta gagtcgatcc gcgaagctgt gggagtaaaa
     6421 ggcattccgg tgattgccac ctcctcaacc tgtacatttg ccctgcgcga cgaatacccg
     6481 gaagtgctga atgtcgacaa caaaggcttg cgcgatcata tcgaactggc aacccgctgg
     6541 ctgtggcgca agctggacga aggcaaaacg ttaccgctga aaccgctgcc gctgaaagtg
     6601 gtttatcaca ctccgtgcca tatggaaaaa atgggctgga cgctctacac cctggagctg
     6661 ttgcgtaaaa tcccggggct tgagttaacg gtgctggatt cccagtgctg cggtattgcg
     6721 ggtacttacg gtttcaaaaa agagaactac cccacctcac aagccatcgg cgcaccactg
     6781 ttccgccaga tagaagaaag cggcgcagat ctggtggtca ccgactgcga aacctgtaaa
     6841 tggcagattg agatgtccac aagtcttcgc tgcgaacatc cgattacgct actggcccag
     6901 gcgctggctt aaactccttt ctgatgcccg gtaagcatgt ggttaccggg catttttgcg
     6961 tacacgattc cgtgcccaat gtatgcgttg caacgcagtg aaaattcctc tgaaaacgtc
     7021 tcgcaaaggc tgaaactggc agatgtcaaa ggcctgggat aaccgtaatg tcgcgtcatc
     7081 ataaatatca ggtgacggac aaccatgacc gaatcaacaa cctcctcccc gcatgatgcg
     7141 gtatttaaaa cctttatgtt cacacccgaa accgcacggg attttctcga aatacatttg
     7201 ccagaaccac tgcgcaagct ttgcaacctg caaaccttac gcctggaacc cactagtttt
     7261 attgaaaaaa gtttacgcgc ttactactcg gatgttttgt ggtccgtgga aaccagcgac
     7321 ggtgacggct atatctactg cgtgattgaa catcaaagct ctgcagaaaa gaatatggct
     7381 tttcggctaa tgcgctatgc cactgccgcc atgcagcgtc acctggataa aggctatgac
     7441 agagttccgc tggtggtgcc attgctgttt tatcatggcg aaacatcgcc ctacccgtac
     7501 tcacttaact ggctggatga gtttgacgat ccgcaacttg cccggcagtt gtacaccgaa
     7561 gcttttccgt tggtggatat caccatcgta cctgacgatg agatcatgca acatcggcgt
     7621 atagctctgc ttgaactgat tcaaaagcat attcgcgacc gcgatttaat cggtatggtc
     7681 gacaggatca ccacgctttt ggttaaaggc ttcactaatg acagccagct acaaacactg
     7741 tttaattatc tgctgcaatg cggcgatacc tcccgtttca cccgttttat tgaggagatt
     7801 tccgaacgtt caccactaca aaaggagaga ttgatgacta ttgctgagcg gctacggcag
     7861 gaaggacacc aaattggctg gcagaaaggt atgcatgaac aagccattaa aattgctttg
     7921 cgcatgctgg aacagggcat tgatcgtgac caggtgctcg cggccaccca gctaagcgaa
     7981 gccgatctgg cagcgaataa ccactaatta acacaggccc acagccgatc cccatgggcc
     8041 tttgatatca ataactacct tttatgcgtg gcccattttt gcctgattta aacatcgcca
     8101 gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac gccaacagcg acaaagttcg
     8161 ctccccacgc caggcattgc tgcgccatat caggagccac agccagaaaa ccagccgctt
     8221 tacccgcagc acggatccgc cgaatgctgg tttcaataat tcgctgcact tccgggtgcc
     8281 cggcgttatc cgggtagccc aacgacgcag aaagatccgc tggtccaata aacacgccat
     8341 caatcccttc gacgtcgagg atctcgtcca ggttatccag tgccgtttta ctttccacct
     8401 gcactaacag acaaagcgaa tcgttgactt gcgccatgta attctcaatg cgtccccagc
     8461 gcgcagcccg tgccacactg gccccaacac cacgctcacc gtagggagga tagcgcgtgg
     8521 cagacgccac ctgacgagcc tgatcggcag tatcgaccat cgggatcaat agagtttgcg
     8581 cgccaatatc cagaacttgt ttaatcagcg atttactgcc ttccaccgga cggatcacgg
     8641 gttggctggc atagggcgct accgcctgta gctggtgata aagatcctga atggtgtttg
     8701 gcgcgtgctc cccgtcaatc agcaaccagt cataaccgga agtagcggca atttctgcca
     8761 tataagaagt agtcgagctt aaccacagac caatttgcac ttcgcccttg cgtaaacgtt
     8821 ctttaaaggg attggttaat aatgcgttca tcgtgttcct taacctattt aatgatgtgc
     8881 cacgtcggtc tgcgcaacag tacgatttac acgcagagtg aaaataatca gcgaaccgat
     8941 gaccgccacc gctgccagtg tcagtaatcc tgccgcatcg ctggcaaata acgtttctgc
     9001 tttcacgcgc aggatcgggg caataaaccc gcccactgca ccaaacaggt ttacaaaacc
     9061 aatccccgca gccagcgccg taccggaaag cagttgtgtc ggcatcgtcc agaacaccgg
     9121 ctgcacggca ataaacccga tagctgcaac acacagcgct acgatcgcca ttactggaga
     9181 aagcagcccg gacagaccaa taccaatgcc cgccgccagt aatgtcagcg ctgcgacatt
     9241 acgccgttcg ccggttttat cggaatagcg cggaataagc caggtcccaa acaaggccgc
     9301 aacccacgga atggcggtga ccaccgacgc tgtaaagccc acttttgttc ccagcaatgc
     9361 cgcaacctgg gtcggcagga agaaaattaa tccgtaaacc gccacctgaa tggtcaggta
     9421 gataatcgct agttgccaga ctcggccatt acgcagcgca tcgctcagcc gagaagtcac
     9481 tttctgttgt tcttcacttg ccagttgatt gataagcaac gttttttctt gtttactcag
     9541 aaaacgtgcc tgctccggtg tgtcatcaag ccaaaagaat gtgaataccc cagcaccgac
     9601 tgccaacaat ccttcaatca caaacatcca gaaccagccg ggatgcccca taaatccatg
     9661 catctccaac agcgcgccgg aaagcggtga tcccagtgtt aacgccagcg gtgcgcccat
     9721 atagaacagc cccataatgc tggcgcgatt acgctgcgga aaccattgcg aggtgagata
     9781 aatcatacca gggaaaaatc cggcctccgc agcaccaagc agagtgcgaa ctatcagaaa
     9841 tttcgcttca gtatcggccc atgccatggc tgccgaaaga aatccccaca gcagtgttgt
     9901 cgtaccaatc caggttctgg cccccagttt gcgcatcaaa agattcgccg gaacacccag
     9961 aaacgcatat accacaaaga aaatgcctgc tcccagcgca taagcttcat tactcaaccc
    10021 ggtatcaatc tggtaggtct gtttggcaaa accgatattc gaacggtcga gaaacgccag
    10081 cacatacagc gccaacataa acggaattaa acgcacacgg tttttcttca ccacggcgtc
    10141 aagcaaagcg gtgctcataa aaagctcctt agaatatgtg ggcgttaccg ttgtcacagc
    10201 aacgcccgga gtgattagtg gctgtagggg cgtttcaaat tgcagtcacg attgagttcg
    10261 acgccaaaac cgggtttatc aagcactgat ttatgaatac gaccattcac cggaaccggc
    10321 tcattgagca gaatcgggtc aaactgcgga cgcatcgttg aacaatccgg gctggtcatc
    10381 aggaattcgc tgaatggcgt attggtgaag gtgatcaccg catggtgaga gtaaacagac
    10441 gaaccgtgcg gcaccaccag ttgccccctg gatttggcga ttgcggcaat ttccaccagc
    10501 gtggttaaac caccgcacca gccaacatcc ggctgcataa tgtcgatacc ggtttctgaa
    10561 agcgtacgaa aagattgcag tgtgccgtgg tgctcaccgc tggtgaccat cattccgact
    10621 ggcgcgttgc gtttcagttc gcgataacct tcatactgct gtggcggcag gcactcttcg
    10681 atccatttca ggttataggg cgcgcaagcg tgggccagtt tggtggcata gttcacgtcc
    10741 tgactcatcc agcagtcgag cattaaccag aaatcctcac cgcatttttc acgcatatcc
    10801 gcgaccataa cggcatcttt gcggatcccc gcatcgccat catgtggccc ccagtgcgtc
    10861 ggcattttgc caccgataaa gcccatctct tttgccagat ccggacgcgc gcctgtggcg
    10921 tagaactgaa tctcatcacg aactgcgccg cctaaaagtt tataaaccgg aagcccgacc
    10981 actttgccga acagatccca cagagccaga tcgacacagg aaatcgtgtt catcaccagg
    11041 ccaccagagc cggagtaata cagggtggca ttgagcattt gatcatggat cagtttgata
    11101 tcactgacac atttaccctc aatgaaacgg ttaagatgtt tttcgacaat aaaacagccc
    11161 atttcaccgg ctgtcgaaac agcgaatccg gtctggccgt tttctgcttc gacttcaaca
    11221 atcaacgtgc caagaacgtt aatgccaaat gactggcgtg actgctcgta atcgcggtat
    11281 ttactcatcg gggtggcaat atgatcgtca atccagtgat tcgccccctg gtcgtgataa
    11341 tcaccgcgcc agcacctttt tctgctgtcg caccgccagt aaaccaggcg cgaacctgtt
    11401 tgatttttgg tagggtcatg atgttctcca ttgttatgag gcctgtaagt caaagggact
    11461 tttccatccc aacagacgtg aaatatccct ggcgcaggca atggccttgc ccgccagata
    11521 atcacggtat tcttcattga tttgtaagcg ggtaccgacc accgagatcg cagcggtaag
    11581 ctcgttattg gcgttaaaca ccggcgcagc gacacaacgg acatcggggt aatcttcgcc
    11641 gttgtcatag ctccagccct gacggcgaat acgcgccagt tcttcgtgaa gttgctgtgg
    11701 atgagtaatc gttgtgggtg tcgcctgctc ccagaccagc ccttcgataa tactttgctg
    11761 taccgctgca ggttgccagg caagcaggca tttacctatt ccggagcgat aaagcgaaag
    11821 gctttttcct tcatgggaac gcacgctgat agtggctgat gactccactt ttaaaatgta
    11881 ataagcgctg ccgttatcaa taatccccag gtgacataac agcccggtgg tatccatcag
    11941 ttgcgtcaaa cgcggtcgag ccagttcccg gagatccatc ttgcttaacg catgaccaga
    12001 aagctccacc agcctggtcc acaaacaaaa attttcctgg tgatcaaggc taagaaaacg
    12061 ctgacgcctg agttcattaa gcagcaaata ggccgtgctt ttagggattc ccaatgtgtc
    12121 aatgatcgtc gcagcactac aggggccaat gcgggcaatc aaattgagaa tatcaatagc
    12181 ccgtgtcagg gcgggaactt tgcttgattc caacatactg gactccagtc ttagatactg
    12241 gaatcagtct cgttgctcag ggggagtaaa attgtgaagc gcatcaaatc ccgctcctcg
    12301 tccagcggac tggagaaatt acgcacaaat ttgtgaatca gtgcacggat tttttcttat
    12361 tgagtacaac aggttggaca agttttgccg gagggggaat gtgccggtgc agtaaaccgg
    12421 cacagagtgg cggaatcaga cagataaggt catggactca acaacctcaa tccagccatg
    12481 ctcactggcg atatcctggc cgtttaacca acggcgcagc atattcagtg ccatcattgc
    12541 gcacacttcc tgacggatag ccaggctgta gcgtgtagtg ctgaaacgca cacgcagagc
    12601 gaaagtgccg tctggcgtgg ctagcgcaaa gttgagatgc tcgttctcga aacccgaaac
    12661 agccagtgcc agcccggcaa aatggttggc ccgccgttct gtaatccagt gcgcagtttg
    12721 cgccagggtt tcttcctgtg aaggaaccac ttcacacgcc agcaatggag cacctgcgcg
    12781 agaaagttgc aaagccaata aaccaccggt gaattgctcg ctcaacgtca ggctgaactg
    12841 gcggttttgc aattcgcgac tgatctgcgc gggcagtcct tcggtgcctt cgaaaatcac
    12901 gctctgtccg gcaacacgtt taacgtccag ccacagtttt tccatcgcct gttgctcgct
    12961 tgccggtccg gtgagtttca gttcgatgat cggcattgag gagcgatagc ccattgttac
    13021 gcccggcggc agttgtagag tgtccaggct ttgtgccaga tcgctttccg aacgaccaaa
    13081 ggtagttaaa cgcagacaaa ccggcgactg tggtaaagaa aaacgctcgc gcaggcgcgg
    13141 caggatttcg tgctcgacca tcaccttaaa ttctgacggt acgccgggag taaagaacat
    13201 caggcaacga ttaagccgca cggcaaaacc acaggcggtg cctaccgggt tattgataaa
    13261 ttcagcactg gcaggcagct ccgcttgttt acggttgctc ggtgccatta ctcgtccacg
    13321 ttcgtggaaa tagcgttcca tctctttgag ccaggcttca tgcagcacca ggccttcacc
    13381 ttttgctgtc gcagcggcga gtgcacttaa atcatcactg gtcggtccca gcccgccgtt
    13441 aacgatcagc acatcggcgt gctgactacg ttcgcgaaga atggtgacta agtcatcaag
    13501 gttatccccc accgtattgc ggcgagataa tggcaacccc tgatgaaaga aaaaatcggc
    13561 cagccaggca gcgttagtgt caacgatttg cccgtgtaac acttcatccc cggtggataa
    13621 catttccact tttaacatgg ttatctcctg cttttgttat cgaatcacta taacgcaaag
    13681 ggagataagg cgtagtaaga aactgaatgg cgcgacagaa tgccgcgccg gaagggatca
    13741 gaaactggcg ttaaccccaa catacgggcc gtcagccacg gcgttgtcgc ggttaccgtc
    13801 tttacccgac agattcaggt agcgataacc cgcctcaata ctgactggat gcataatggt
    13861 gtaacgcgcg ccagcattcg cttcctcata acttttaata ccgctggaga gtgaatccgg
    13921 agagtagtaa tactcgccaa acaaacggaa gctgttgcca attttccact gcaaaccacc
    13981 tcctaccgct gcggcataac cttcatcgcc gtaattcggg ttggtgtaaa cgccttttcc
    14041 gccaacggtc gccattaacg gcccgagagg cagattcaac cccagcccca cgccagcgac
    14101 gtcaccgtcg tcgtcgttat gtgtccagtt accgcttaac gccaggcccg tcgattcagt
    14161 cccaaaaccg acaccaatat tggtgtattc tttacccgcc tgaccgctga tgctcattgc
    14221 attgaccgat gcagaaacca acagcattcc ggccagacct gctagcgcaa tttttttcat
    14281 tatccccttt cccacaagca attaatcaaa attcccaaag ctgcgggatt gtactgcctg
    14341 gcacagagga ttcaatgagc gaaaaaaggg atctagtcac ttattgtaac taaatacttc
    14401 ttaaataatt aaacgccgaa aaaataaaca cactttaccg atacacacaa aaataataat
    14461 aagtgcagtt atcatcgcaa aagagattac tgtcactttc acatatttct gagttaccgt
    14521 attctcatgg tttcttttta tttataagga atacccctgt gcgacaacgg actattgtat
    14581 gccctttgat tcaaaatgat ggtgcttatt tgctgtgcaa aatggccgac gatcgcggcg
    14641 ttttccccgg tcaatgggcg ctttcgggtg gcggcgtgga gtctggcgaa cgaattgaag
    14701 aggcactacg ccgcgaaatt cgcgaagaac tgggagaaca gctgcttttg acagaaatca
    14761 cgccgtggac cttcagcgat gatattcgca ccaagacgta tgcagatggt cgcaaggaag
    14821 agatttatat gatttacctg atttttgact gcgtttctgc caaccgagaa gtgaaaataa
    14881 acgaagagtt tcaggactac gcgtgggtaa aacctgaaga tctggtgcat tatgatttaa
    14941 atgttgccac ccgaaaaacg ttacgtttga aaggtcttct ttaacaatga tgacagccat
    15001 cactggctgt cagattgaaa atttagtggt taacgaattc cccatccaga taaactttgc
    15061 ctttttccac atgcatgact aaaccatcca gataatcagg tttaaatgtc gcatcacgct
    15121 tatttttagc aatatatgtc aggcaatgat tatgggtgaa aataacgata tttttatcag
    15181 gcgctttgct ttgtaagtcc ttaattgcac tataaatctc attaccgcac tgaagaagtc
    15241 gtttatctac cgtcagtttt ttacccgcgg aaaaccaggt cgccgactga atggtccgga
    15301 cggtattact ggaataaaga tcgaaatcag ggatgtcagc gctaaaagcg ttgcccagtt
    15361 cacgggcatc ctgggtacct ttaaccgtaa tacctgtttt atctgacaag cattgattgg
    15421 ttgaacgatc gcaacgttca gcatggcgaa acaaaacgac aaccgggtgc tgctgtgcca
    15481 gtctggccaa tgttttattg tcgatacgtg gcaaaccatt gctactccag gcggcatgag
    15541 tacccagtcc ggcaattgca gcgagcgcca gtaaaatgat gaaatatttt tttgacttca
    15601 atgatgagcg gcaaaaagct aacacgtaaa ctccacctat agacaagcgc aaccagacaa
    15661 ttaccgtgaa attgagcgac atttctggcg ataattagca gttggtgtaa tattaaaaat
    15721 cctacgatgt cggcaaaatg cctcaaaatt ttgccaaatg caaagcctaa ataagaaaaa
    15781 atataaaaat ttcaatattt acgtctaata ttagtttctt aaggttaagt taatattcta
    15841 tccttaaaat ttcgctccaa atggcaaaat atacacaaca ctctttatag caaatataag
    15901 tggacaggta ttcaatggcg gaaggaaaag caatgtcaga atttttgcct ttttcgcgac
    15961 cagcaatggg cgtggaggaa ctcgctgcag ttaaagaggt tctcgaatcc ggttggatca
    16021 caaccggtcc gaagaatcag gcgcttgagc aagccttttg ccagttgacg ggaaatcagc
    16081 atgccatcgc ggtcagttca gccaccgccg gaatgcatat cacgctaatg gcgttggaaa
    16141 ttggcaaggg cgatgaagtg attacgcctt ccctgacctg ggtttcaacc ctcaatatga
    16201 tttccttgtt gggtgcaacg ccggtaatgg tggatgtcga ccgcgatacg ctgatggtca
    16261 cgcctgaagc gatcgagtca gccattacgc cacgcactaa agccatcatt ccggtgcatt
    16321 atgccggtgc gccagcagat attgacgcca ttcgcgccat tggcgaacgt tacggcatcg
    16381 cagttatcga agatgctgcc catgccgtcg gtacgtatta caaagggcga catattggcg
    16441 caaaaggtac cgctattttt tcatttcatg ccattaaaaa tattacctgt gcggaaggtg
    16501 gcctgattgt aactgataat gaaaaccttg cccgccagct acggatgctg aaatttcacg
    16561 gtctgggtgt cgatgcctat gacagacaaa cctggggccg tgcaccgcag gctgaagtct
    16621 taacaccggg ctataagtac aatctgaccg atattaacgc cgcgattgcc ctgacacagt
    16681 tagcaaaatt agagcacctc aacacccatc ggcgcgaaat tgcccagcaa tatcagcaag
    16741 cactggcagc tctccccttt cagccattaa gccttcccgc ctggccgcac gttcacgcct
    16801 ggcatctgtt tattattcgt gtcgatgaac aacgttgtgg tatcagtcgc gatgcgttga
    16861 tggaagcgtt aaaagaaaga ggcattggta ccgggttaca tttccgcgcc gctcacacac
    16921 aaaaatatta tcgcgagcgt tttcccacgc tgtcgttacc gaataccgaa tggaatagcg
    16981 aacgcatctg ttctttgccg ctgttcccgg atatgactac cgccgatgcc gaccgtgtca
    17041 tcacagccct tcagcaactc gcaggacaat aagccatgtt tgaaatccac cctgttaaga
    17101 aagtctcggt ggttattccc gtttataacg agcaggaaag cttaccggaa ttaatcaggc
    17161 gcaccaccac agcctgtgaa tcgttgggga aagagtatga gatcctgctg attgatgacg
    17221 gcagtagcga taattccgcg catatgctgg tcgaagcctc acaagcggag aacagccata
    17281 ttgtgtctat tttgcttaac cgcaattacg ggcaacattc agcgattatg gcgggattca
    17341 gtcacgttac tggcgactta attattaccc ttgatgccga tctccagaat ccgccagaag
    17401 aaatcccccg cctggtggca aaagccgatg aaggttacga cgtggtaggg actgtacgcc
    17461 agaaccgcca ggacagctgg tttcgtaaaa cagcttcgaa gatgattaac aggcttattc
    17521 agcgcaccac cggcaaagcg atgggtgact acggttgtat gctgcgcgcc tatcgccgtc
    17581 atattgtcga tgcgatgttg cactgccatg aacgtagcac ctttatcccg attctggcga
    17641 atatcttcgc ccgccgtgcc attgaaattc cagtacatca tgccgagcgt gagtttggtg
    17701 aatccaaata cagttttatg cgcctgatta atttgatgta cgacctggtg acctgcctta
    17761 ccacaacgcc gctacgtatg ctgagtctgc tcggcagcat tattgcgatt ggaggtttta
    17821 gcattgcggt gctgctggtg attttacgcc tgaccttcgg accacaatgg gcggcagaag
    17881 gcgtctttat gctatttgcc gtgctgttta cttttattgg cgctcagttt atcggcatgg
    17941 gattgctcgg tgaatatatc ggcaggatct acaccgatgt ccgcgcccgc ccccgctatt
    18001 ttgttcagca agttatccgt ccatccagca aggaaaatga ataatgaaaa ccgtcgtttt
    18061 tgcctaccac gatatgggat gcctcggtat tgaagctctg ctggctgccg gttacgaaat
    18121 tagcgccatt tttacccata ccgataatcc cgctgaaaaa gccttttatg gttcggtggc
    18181 tcgtctggcg gcggaaagag gcattccagt ttatgcgccg gataacgtta atcatccgct
    18241 gtgggtggaa cgcattgccc aactctcacc agaggtgatt ttctcttttt attatcgcca
    18301 tcttatttgc gacgagattt tgcagctcgc tcccgcaggt gcatttaatc tgcacggttc
    18361 gctgttacca aaatatcgtg gtcgcgcgcc gctgaactgg gtgctggtga acggtgaaac
    18421 ggaaacgggc gttacattgc accgaatggt aaaacgtgcc gatgctgggg ccattgtggc
    18481 gcaactgcgc gttgccattg cgccagacga tatcgctatt acgctgcatc ataaattgtg
    18541 ccatgccgcg cgccagctac tggaacagac attacccgcc attaaacacg gtaatattct
    18601 ggaaattgcc cagcgcgaaa acgaagccac ctgttttggt cacagaacgc cagatgacag
    18661 tttccttgaa tggcataaac cggcatccgt actgcacaac atggtacgtg ccgttgccga
    18721 tccgtggccg ggtgccttca gctatgttgg caatcagaaa ttcaccgtct ggtcgtcgcg
    18781 tgttcatcct catgccagca aagcacagcc ggggagcgtg atttctgttg cgccactgct
    18841 gattgcctgt ggcgatggcg cgctggaaat cgtcaccgga caggcgggcg acggcattac
    18901 tatgcagggc tcgcaattag cgcagatgct gggcctggtg caaggttcac gcttgaatag
    18961 ccagcctgcc tgcaccgccc gacgccgtac ccgggtactc atcctcgggg tgaatggctt
    19021 tattggcaac catctgacag aacgcctgct gcgcgaagat cattatgaag tttacggtct
    19081 ggatattggc agcgatgcga taagccgttt tctgaatcat ccgcattttc actttgtcga
    19141 aggcgatatc agtattcatt ccgaatggat tgagtatcac gtcaaaaaat gtgatgtcgt
    19201 cttgccgctg gtggcgatag ccacgccgat tgaatatacc cgcaacccgc tgcgcgtatt
    19261 tgaactcgat tttgaagaga atctgcgcat tatccgctac tgcgttaagt accgtaagcg
    19321 aatcatcttc ccgtcaactt cagaagttta tgggatgtgt agcgataaat acttcgatga
    19381 ggaccattct aatttaatcg tcggcccggt gaataaacca cgctggattt attcggtgtc
    19441 aaaacaatta cttgatcggg tgatctgggc ctatggcgaa aaagagggtt tacagttcac
    19501 cctcttccgc ccgtttaact ggatgggacc acgactggat aaccttaatg cagcgcgaat
    19561 tggcagctcc cgcgctatta cgcaactcat tctcaatctg gtagaaggtt caccaattaa
    19621 gctaattgat ggcggaaaac aaaaacgctg ctttactgat atccgggacg gtatcgaggc
    19681 gttgtaccgc attatcgaaa atgcgggaaa tcgctgcgac ggcgaaatta tcaacattgg
    19741 caatcctgag aacgaagcga gcattgagga actgggcgag atgctgctgg cgagcttcga
    19801 aaaacatccg ctgcgccatc acttcccgcc gtttgcgggc tttcgtgttg tcgaaagtag
    19861 cagctactac ggcaaaggat atcaggacgt agagcatcgt aaaccgagca tccgcaatgc
    19921 ccgccgctgc ctggactggg aaccgaaaat tgatatgcag gaaaccatcg acgaaacgct
    19981 ggatttcttc ctgcgcaccg ttgatcttac ggataaacca tcatgaccaa agtcggctta
    20041 cgcattgatg tcgatacctt tcgtggcacc cgtgaaggcg tgccacgtct gctggaaatc
    20101 ttgagtaagc ataatattca ggccagcatt tttttcagcg tcggcccgga caatatgggc
    20161 cgccatctct ggcgactggt gaagccacag tttttgtgga agatgctgcg ctcaaacgcg
    20221 gcatcgcttt atggctggga tattttactg gcaggtacgg cctggccagg taaagagatt
    20281 ggtcatgcca atgccgatat cattcgtgaa gcggctaaac atcacgaagt cggcctgcac
    20341 gcctgggatc accatgcctg gcaagcccgt agcggtaact gggatcggca aacaatgatc
    20401 gacgatattg cacgcggtct tcgcactctg gaagagatta tcggtcaacc ggtaacctgt
    20461 tctgccgctg cgggctggcg tgccgaccag aaggtgatcg aagcaaaaga agcgttccat
    20521 ttgcgctaca acagcgattg tcgtggggcc atgccgttcc gtccactgct cgaatcagga
    20581 aaccctggca ctgcgcaaat tccggtgacc ttacccacct gggatgaagt gattggtcgg
    20641 gatgtgaaag cagaagattt taacggttgg ttactcaacc gcatcctgcg agataaaggc
    20701 acgccggttt ataccattca tgcagaagtt gaaggctgcg cttatcagca taattttgtg
    20761 gatctcctca aacgcgcagc tcaggaaggc gtgacatttt gccctttaag cgaactgtta
    20821 tcagagacgt tgccgctcgg acaagttgtt cgcggaaata ttgccggacg tgaaggctgg
    20881 ctgggttgcc aacaaattgc gggtagtcgc tgatgaaatc ggtacgttac cttatcggcc
    20941 tcttcgcgtt tattgcctgc tattacctgt taccgatcag cacgcgtctg ctctggcaac
    21001 ccgatgaaac gcgttatgcg gaaatcagtc gagaaatgct ggcatccggc gactggattg
    21061 tgccccatct gttagggcta cgttatttcg aaaaacccat tgccggatac tggattaaca
    21121 gcattgggca atggctattt ggcgcgaata actttggtgt gcgggcaggc gttatctttg
    21181 cgaccctgtt aactgccgcg ctggtgacct ggtttactct gcgcttatgg cgcgataaac
    21241 gtctggctct actcgccaca gtaatttatc tctcattgtt tattgtctat gccatcggca
    21301 cttatgccgt gctcgatccg tttattgcat tctggctggt ggcgggaatg tgcagcttct
    21361 ggctggcaat gcaggcacag acgtggaaag gcaaaagcgc aggattttta ctgctgggaa
    21421 tcacctgcgg catgggggtg atgaccaaag gttttctcgc ccttgccgtg ccggtattaa
    21481 gcgtgctgcc atgggtagcg acgcaaaaac gctggaaaga tctctttatt tacggctggc
    21541 tggcggttat cagttgcgta ctgacggttc tcccctgggg actggcgata gcgcagcggg
    21601 agcctgactt ctggcactat tttttctggg ttgagcatat tcaacgcttt gcactggatg
    21661 atgcccaaca tagagctccg ttctggtact acgtgccggt catcattgcc ggtagcctgc
    21721 cgtggctggg attactcccc ggtgcactgt acacaggctg gaaaaaccgc aagcattccg
    21781 caaccgtcta tttgttgagc tggacgataa tgccgctgct gtttttctcc gtcgctaaag
    21841 gtaaattgcc cacctatatt ctttcctgct ttgcatctct ggcaatgctg atggcgcatt
    21901 acgctttgct ggcagcaaaa aataatcctc tggcgctgcg gattaatggc tggattaaca
    21961 tcgcttttgg cttcactggc attattgcca catttgtggt ctccccgtgg ggaccaatga
    22021 acacgccggt gtggcaaacc ttcgagagct ataaagtctt ttgtgcctgg tcgatttttt
    22081 cgctatgggc atttttcggc tggtacacct taacaaacgt cgaaaagacc tggtcttttg
    22141 ccgcgctttg cccgctgggg ctggcgttgc tggtaggatt ttcaattcct gacagagtca
    22201 tggaaggaaa acatccgcaa ttttttgtcg agatgacaca agaatcactt cagccaagcc
    22261 gctatattct tactgatagc gtcggtgttg ccgcaggtct ggcatggagc ctgcaacgcg
    22321 atgacatcat catgtatcgc cagacaggtg agttgaaata cggccttaat tatccggatg
    22381 cgaaagggag atttgtcagc ggtgatgagt tcgcaaactg gcttaatcaa catcgtcagg
    22441 aggggattat tacactcgtg ctttcggttg accgcgatga agatatcaac agtctcgcca
    22501 ttccgcccgc agatgccatc gatcgtcagg agcgtctggt gctgattcag tatcgtccca
    22561 aatgatctgg ctaacattag tctttgccag cttgcttagc gttgccgggc agttgtgtca
    22621 gaaacaggca acctgctttg tggcgataaa caaacggcgc aaacatatcg tgctgtggct
    22681 gggactggcg ctggcttgtc ttggtcttgc catggtgctc tggctgctgg tcttgcagaa
    22741 cgtaccggta ggcattgcct acccgatgtt aagtctgaat tttgtctggg tgacgctggc
    22801 tgcagtaaaa ctgtggcacg aaccggtatc gccgcgtcac tggtgtgggg tggcgttcat
    22861 tattggcggc attgtgatcc tcgggagtac ggtgtaatgg gcctgatgtg gggattattc
    22921 agcgtcatta ttgcctcagt tgcgcaatta agcctgggtt ttgcggcgag tcatctgccg
    22981 ccgatgacgc acttatggga ttttatcgcg gctctgttag cttttggcct ggatgcccgg
    23041 atactgttac tcgggctgct gggttatctg ctttccgttt tttgttggta taaaacgcta
    23101 cataaacttg cgctaagcaa agcctatgcc ttgctcagta tgagttatgt gctggtatgg
    23161 attgcttcaa tggtacttcc cggctgggaa gggacttttt cgctgaaagc actactggga
    23221 gtagcctgta ttatgagcgg gttgatgctg attttcctgc ccacgacaaa acaacgttac
    23281 tgagttttcc ctgccacttt acactgccgt tcccactcat cggggctata actgcttgcc
    23341 gagaggactt tcaccgtatg cagcttttca cgattaatac aataaagcgc attctgcaaa
    23401 ggcgagagta aatcccccac tttcacggcg aagtcactct tcacttcggc gatcattttt
    23461 atcgcgccgc cagcatcgca gagcataagt acatgtctgt tatcttgttt attgcagcac
    23521 gattttttga ccagccattc cattgcatta tcctgtttgc taagagtttt cacatcaatt
    23581 tacagattaa cagataaacc tattatcaac ggcaatggaa tggcaaaatt gcgaagtgaa
    23641 actaatgatt tcgtaatcct ggcgttgcac ccactctttt aacgcctgac gtgaaatttt
    23701 aataccgccg tttttcagct ccggcggtaa agttagccag cgcaccggtt gttgaaaacg
    23761 agccagctta tctttcaccc attcgccaag atcaacggtt tgctggtcat actccaccac
    23821 cgctaccggt cgatgaccaa actccttgtc ggcaacgggg acgataaaca cctgcaaaac
    23881 cgcaggatgt gcagcaatta cgcgctcgac ttcttccggc tgaataccct ctccgccact
    23941 gaagaacaga ttgtctaaac gtccgacaat ggtcagcttg ccattatgca tctcaccgcg
    24001 atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa accagttgcc cgttacgcca
    24061 gtaaccttct gccatactgg cagcccgcag ccacacttca ttattaacga ttttcacttc
    24121 ccgacccggc agcggcgaac caacgtctgc caggccgtcg gcttctttcg cacacaccgt
    24181 ggaggcaaac tcggtcagac catagccgca aaagcaacga atcccctgct cgcgcgcctg
    24241 ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac accgctttca gggaaacgga
    24301 actgcggtta accagcaaac gccagagttg tgttggcacc agtgaagcgt gagtacagcc
    24361 tgccagcatt tgctccaatg gctgtttatc acgcaccgtc atccgcgcac cagcgtataa
    24421 ccagcgccac ataattccct gaccggagac gtgaaacagc ggtaaagaga gcaaccagtc
    24481 atcgtgatcg ccaaacggaa tcagcgataa cacaccttgc gcactggcaa gatgggcctg
    24541 ataagtatgg acagcggctt tcggcaaacc ggtagaaccc gaggtcaacg tcattgagca
    24601 cagacgcgtc ggctgccacg tagcggcatg tgcgccttca acccgctgaa tgtgcagcga
    24661 cgttaatgcc ggaaacgtgt tttccccatc cggcaccagc gcaaattgca gcgtcagatt
    24721 gggcagcaat tcttcaagca acggttgcgg cagctgaggg ttcacgggca acacccgcgc
    24781 cccgcattgc agtaacgcca gccaggcgag cagcgtttgc ggcgtattcc acgcccgcaa
    24841 catcacgccg ctgccctcaa ccacaccctg cgccgcaaag cccgtagcta actcatcgac
    24901 gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta agacgtaagg cgatggcttc
    24961 tccccgcact tgccgccagt gacgccacgg ccagtcagag aagatcataa caactgctcc
    25021 agtacatcag cgtccactaa cggcagtggg ctccccggcc agcgacgaac ctgctgcgcc
    25081 tgcatcagat ccagcgtgtc cagtcccgga atggtgtctg gcgttaacca ggcagcaatc
    25141 cgcgccagtt gcgttagacc taagctcgac tcaatggaag aactgatcac cgccgtcagc
    25201 cccagcgcat gcgccgcctg tacctgctcg cgtacttttt ccagactgcc cgtgagcgtg
    25261 ggtttgataa ctaccgcgcg cacgccctct tcagccacaa aggcaaaatc cggctcgcgc
    25321 aggctttcat cccaggcaat ggcaatgccg gtttcacggg caaacgctcg cgaatcatcg
    25381 cgggttttgc acggctcttc gagaaacgcg atgcggtggc gataatccgg gttaacatat
    25441 ttggcaaact gctgaccttt cagcggtgtc caggcgcgat ttgcgtcaag acgcaaatgc
    25501 agatccggaa ttgcctccag caacagatta accaccatgc cgtcgcgcac ggcttcgtac
    25561 aatcccactt tgaccttcgc cactttctcg cctggcatat cagcaagttt gaggatcaga
    25621 tcgtcaggat cgccattaca cagtggtgcc gcacggtaat tggccgcctg cgggagcgta
    25681 tctgccatct ccgccaatgc acagcttacg ccaaaggcaa cggaaggcat ctgcggtagc
    25741 tcgccatcgc ctgccagcca gttatttacc caagcaagca gcacaatttg cgcctcttcc
    25801 caggtttcct gactgaagcc cggcagtggg gagatctccc cccacccttc gcgctcgcct
    25861 tcacgcaggc aaacatacag cccgtcgcgg gtttttaacc gcctgtcgcg cagaaccatc
    25921 cccgcgtcca tggggatctg ccagcggtat acctgcgcgc tacgcattac ggattccgtt
    25981 taaatttgct gaaatcaggc tgacgtttct ggttgaaagc gttgcgacct tcctgacctt
    26041 cttccgtcat gtagaacagc atggtggcgt tgcccgccag ctcctgcaga cccgcctgac
    26101 cgtcacagtc ggcgttcagc gcagctttca ggcagcgcag cgccatcggg ctgttttgca
    26161 gcatttcgcg gcaccaacgg acggtttctt tttccagatc cgccagcggt accacggtgt
    26221 tgacaaggcc catatccagc gcctgttttg catcgtactg acggcacagg aaccagattt
    26281 cacgcgcttt tttctgtccg acgatgcgag ccatgtagga agcgccccag ccgccgtcga
    26341 aggaaccgac tttcgggcca gtctgaccga agatggcatt atctgccgcg atagtcaggt
    26401 cgcacatcat gtgcagaacg tgaccaccgc cgatggagta gccagccacc atcgcgacaa
    26461 ccggtttcgg acaggtacgg atctgacgct ggaagtccag cacgttcagg tgatgtacgc
    26521 cggaatcatc tttatagccg ccgtaatcac cacgcacttt ctggtcacca ccggagcaga
    26581 acgctttatc gcctgcgcca gtcagaatga tcacgccgac gttgtcgtca taacgcgcat
    26641 ctgccagtgc ctggatcatc tcttttaccg tcagaggacg gaaggcattg cgcacctgcg
    26701 gacgattaat ggtgattttt gcgataccgt cggtggattt ttcataacga atgtcctcaa
    26761 aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaaag cattgcttca tcaggataaa
    26821 tcatattgtg tcctttagtc agaaacgcaa gatctgcgcc agacttgcga taacgccagc
    26881 gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg acatggcagt cggcagccag
    26941 ttccgccgcc aaggcgcgga atttgctgtc acgttcacca cataaataat aaaacgcaaa
    27001 tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg gcgagagaag tcgcctccag
    27061 catggcggca agggttgcgc cattattgtt actgcgcagc gccaccagct cccggcgttg
    27121 atcgtcattg agcgaggcaa aaacaggctg ttgataccag tcggcaaata ccgccgttaa
    27181 cggttctgtg cgaaaacgct gcgcccattg gcgatcggaa cgctgacgtt ccgcgcgttg
    27241 ttcagcattt tgcaacccag gatgcccgcc ttcgacaaca accccacaaa gccccggcat
    27301 cccctggcaa gccgccatca tcgccacccg tcccccaagc gagtacccca ccaaccagaa
    27361 gttaaggatg ttgtaactaa ccagggtttt acgcagtaag tcggtgacat catcaaatcc
    27421 atcgacgcta atcgccgccg aaccaccgtg acctgggaga taaacataca accgtgagta
    27481 gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa tcgccggaaa aaccgtgcaa
    27541 aaacaccagc cagggtaaac ctggtttgcc gtgttttgcc tgcgcgtgca ggatcataaa
    27601 tggcttacct gcgccagaag ttgctggagc gtttgcgcac catcggtgtc gttaaccacc
    27661 atttcaatta ccgtggtggt tggcgtgcgc caggcatcgg caagtgccgt ttcaagttcc
    27721 tgccagtttt gcggacgatg atatttcagc tcgaacatcg cggcggcgtg ctcaaaatgg
    27781 acgttttgcg gcatcagata gaaacgctcg cgctcgcttt tcggcgttgg cagcagcgag
    27841 aaaatttgcc cgccgttgtt gttcaccaca attaatatca gcggcgcgga aacctgacgc
    27901 aataacgcca gcgcgttgag atcgtaaagt gcggagagat cgcccacaat cgccagcgtc
    27961 ggtttgccgc ttgcccgctg aacgccggcg gcagtcgaga gcagcccgtc gataccgctg
    28021 gccccacggt tgctgtacac cgggtaacct gccggaagtt gcgaaagcgc atcaatcaga
    28081 cgtaccacca ggctgttacc gacaaacaat tgcccctgtt caggcagata gtcgctgatg
    28141 cgatgcgcca gttgcgcttc gccaaacgca tcgcgacggg caataaccgc ctgcattgcc
    28201 tgttccgcca ggcgcgggat ttcaacgcac cagggctggc gtttttctgc cggatgcagc
    28261 tccagccagt cggcaatatt ggcaattaag cggcgtccac gatggtgcgc cggatcaagc
    28321 cgcccttcga tgtcatccac aatccagtac tcttcgggtt cacagcttgc ctgccattgc
    28381 aggagccgtt tgcccgtcag gctgcttccc agttgcacca caatttgcgc ctgttgcagt
    28441 tcgctggtcg ctttggcatt gcctaaccag agatcggcac acggcagcgg ctgtccggtt
    28501 tgcgacagca cgtcgccaat cagcggccag ccaagagttt gcgcccacag tgcaactttt
    28561 ttgccctctt ccgcactcat gcgcccggca accaccacgc cgcgtttttg tcgccagaag
    28621 aaccagtcgc gctgtttttc actttccaga cgaggcgctt cacgcagcca cggtttgtcg
    28681 tcctgccacc agtcgcccag ccgctgttgc cagctaagcc cggtgtcgtc catttcgcca
    28741 tacagcggtt cggcaaacgg gcagttgata tggactccac cagaatgaag cgtaccgaga
    28801 gcatggtcga tggtagaaac cagccaacgt gcggggatat cctgggtcgg gcgcggcaat
    28861 gaaatactgt gcgtggggtg agaggcgaac attcccggct gacgaatcgc ctgattcgcg
    28921 ccgcagtcaa tcagctccgg cgggcgatcg gcggttaaca ggatcagttt ttctccggtt
    28981 aacccagctt caatcagtgc cggatagaga tttgctaccg ccgtaccgga ggtcacaatc
    29041 accgccaccg gctgcttgct gactttcgcc agccccagcg ccagatgccc caacccacgc
    29101 tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg ccgccgctaa cgttaacggt
    29161 gtagaacgcg agccaggggc gatacagatg tgtctgacgc cgtgacgcgt taatgcttcc
    29221 agaatgaccg ccgcccagcg tcggttaaat gcgcttactg acatgagttt gtccggtatc
    29281 aatattgcgg ctaagtataa ggagctacaa aaatagatta ttgatatgaa tcggtaatga
    29341 tgcgactcat tactattcca tctgtaataa agtacgcagc cctgccgctt tgttgtcgat
    29401 ttcctgccac tcttgctcgg ggtcggaacc acggacaatg cccgcgccag catataatcg
    29461 cacgacattg ccgctaattt ttgctgagcg cagggaaacg cagaattcgc tttgctgtaa
    29521 tgagagatag cccgcagaac cggcgtacca ttctcgggtg aacggttcgt gacgggcgat
    29581 aaactgtcgc gccagatcgc gcggtaagcc agcaactgct gccgttggct gcaactgatg
    29641 taaacagatc acattatccg ctttgttgag tgaagtccag atacagcggc gaagatgctg
    29701 cactttacgc agacgcagta cctgcggcgg taaaacatcc agcgtctggg tatcggcctg
    29761 taaccgttgg cagatatctt ccaccaccag catattctcg cgctggtttt tatcatccgc
    29821 catcagccac tcacctaact gctgcgcctg cttatcatca ggatgatttg ctaccgttcc
    29881 cgccagcgct tcagtacgca gcgctttgtc acgccgccgc cataaccgtt ccggtgaaga
    29941 gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa tggtagcaat tcagattcag
    30001 tcgacgactg gcagccatca tcgccgccgc gttgaccgga cttgcgaaat gcaggtcagt
    30061 tgcccgagcg agcaccactt tgtcgagctc accttcggcg atggttttcg ttgccagttc
    30121 gattaattgc gtccagcccg ttttgtccgg ccagtgttgt tctcgcgtgg tggttaaatg
    30181 taacccaggc aagggcttga tactcaccag tgtggcgata aattctcttg ccttaatcgc
    30241 atcgtgctga agggagcttt cgctgaatag cgtcagccgc agcgtggctt taccgccaca
    30301 gcgtcgccat tccaggcgtg gtaaaagtaa attgccctgc gacgggtcaa acgcattcag
    30361 cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga agaaaacgtt gtgcctggtc
    30421 caacgacgta aaacgggtaa tcgcgcccag gacggcagct tcttcatcac cattacgttg
    30481 ttgccagtag aattgcgggt atgtttgctg actggccagc cagctcaagg catcaaaagc
    30541 gtctttgaga gggaaaggaa tatcgataac ccgaatgccg ggtgtcgccg gaatctcttg
    30601 cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt gattgcacgc ggacctctcc
    30661 ctgttaaaag cctcacatta tacggggtac tacaaaaaaa atgcagtacc ccggtgtagg
    30721 gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc ccaacggccg cgcccacacc
    30781 aattccttgc cagggttttt cgtggacgta gtcatcagca cgataaactg cctgcttcgc
    30841 ccgatagtaa taactgtctg aagcctggct aacccgtttt ttcacatcat ccagcgcttt
    30901 ttctgcacgc gctttcagct caacatattt ctgatcggcg ggatcgccag aggagcggag
    30961 cacctcttcc agtgtttcac taagcagcgt caggtcgtca tcgatacgtg tatcaccaaa
    31021 ctgattagac attctcgttc tccatttgcg taaaacctgt cactaactat agacaagggt
    31081 tgttgattac gcctgaatta cctcgcgtgc catcccaatg tgtgggatac catcttcttc
    31141 atagacctcc gtcacaggga taaaaccgaa actctggtag aagttttgca gatgcgcctg
    31201 cgccccgagg taaacaggct tatcaggcca gtgatgcgtg caggtgtcga gggttttact
    31261 catcagttgc tgccccactt tttcgccacg caacgcttca ctgacaatca cccgaccaat
    31321 aacgaccggc tcaagatcat catcactttt cagaatcctc gcatatgcca ccagttcatc
    31381 atttttccaa ccgagaatat ggcgattatc ccccgtcaaa tcatcaccat cgatatcctg
    31441 ataagggcaa ttctgctcga caacaaacac cgcgcaacgc agttgtaata aggcataaag
    31501 ctgggaaacc gataattcag agtgatgcag atcttaccat tcaatcataa tgctctcctt
    31561 ttagatgtat gccttatact agccggaata ttttttgaaa cgtgatgaac tcatggaatt
    31621 aattttttta ggtacttcag ccggtgtgcc aacccgcacg cgcaatgtca cggcaatatt
    31681 gcttaacctg caacatccga cccagagcgg actttggttg tttgactgcg gtgaaggcac
    31741 ccagcatcag ctactgcata ccgcctttaa ccctgggaaa ctggacaaga tttttatcag
    31801 tcaccttcat ggcgatcatc tctttggttt acccggcttg ctgtgcagtc gttctatgtc
    31861 aggcattatc caacccttaa cgatttatgg tccgcaaggt atccgtgaat ttgtggaaac
    31921 cgcgctgcgg attagcggct catggaccga ttatccgctg gaaattgtcg aaattggcgc
    31981 tggcgaaatt ctcgatgatg gcctgcgcaa agtgaccgct tatccgatgg aacacccact
    32041 ggaatgttat ggctatcgta ttgaagaaca tgataaaccg ggtgcattaa atgcccaggc
    32101 attaaaagct gctggcgtgc cgcctggccc actgtttcag gaattaaaag cgggcaaaac
    32161 aatcacgctg gaagatggaa ggcagattaa cggcgcagat tacttagctg ctccagtgcc
    32221 aggtaaagcg ctcgctattt tcggcgatac cggcccctgc gatgccgcac ttgacctggc
    32281 taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat ataaccatgg aagccaaagc
    32341 caatagtcgc ggccatagct ctacacgcca ggctgcgaca ctagcccgtg aggctggagt
    32401 cggcaagcta atcattaccc acgtcagctc gcgctatgat gacaaaggtt gtcaacacct
    32461 gttacgtgaa tgcaggtcaa ttttcccggc gactgaactg gcgaatgatt tcaccgtgtt
    32521 taacgtttaa ct
//
LOCUS       DAETQN010000040        31699 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.040, whole genome shotgun sequence.
ACCESSION   DAETQN010000040 DAETQN010000000
VERSION     DAETQN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.040"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1133
                     /gene="ypdA"
                     /locus_tag="K0W68_001832"
     CDS             <1..1133
                     /gene="ypdA"
                     /locus_tag="K0W68_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HBI3073408.1"
                     /translation="LVQSVEGEKEASAARQTKLALDIANKTLPLFRHVNSESLRKVCE
                     IIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEA
                     HRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRA
                     EQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE
                     LKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAI
                     VHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNV
                     HHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1148..1882
                     /gene="ypdB"
                     /locus_tag="K0W68_001833"
     CDS             1148..1882
                     /gene="ypdB"
                     /locus_tag="K0W68_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBI3073409.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1895..2752
                     /locus_tag="K0W68_001834"
     CDS             1895..2752
                     /locus_tag="K0W68_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3073410.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2755..5250)
                     /gene="ptsP"
                     /locus_tag="K0W68_001835"
                     /pseudo
     CDS             complement(2755..5250)
                     /gene="ptsP"
                     /locus_tag="K0W68_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5275..6312)
                     /gene="ypdE"
                     /locus_tag="K0W68_001836"
     CDS             complement(5275..6312)
                     /gene="ypdE"
                     /locus_tag="K0W68_001836"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBI3073411.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6312..7397)
                     /gene="ypdF"
                     /locus_tag="K0W68_001837"
                     /pseudo
     CDS             complement(6312..7397)
                     /gene="ypdF"
                     /locus_tag="K0W68_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7412..8659)
                     /locus_tag="K0W68_001838"
     CDS             complement(7412..8659)
                     /locus_tag="K0W68_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBI3073412.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8681..9007)
                     /locus_tag="K0W68_001839"
     CDS             complement(8681..9007)
                     /locus_tag="K0W68_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBI3073413.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9227..10192)
                     /gene="glk"
                     /locus_tag="K0W68_001840"
     CDS             complement(9227..10192)
                     /gene="glk"
                     /locus_tag="K0W68_001840"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBI3073414.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10396..11652
                     /locus_tag="K0W68_001841"
     CDS             10396..11652
                     /locus_tag="K0W68_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBI3073415.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11767..12093
                     /locus_tag="K0W68_001842"
     CDS             11767..12093
                     /locus_tag="K0W68_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBI3073416.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12140..13378)
                     /locus_tag="K0W68_001843"
     CDS             complement(12140..13378)
                     /locus_tag="K0W68_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBI3073417.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13714..14916
                     /gene="nupC"
                     /locus_tag="K0W68_001844"
     CDS             13714..14916
                     /gene="nupC"
                     /locus_tag="K0W68_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBI3073418.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(14966..17154)
                     /locus_tag="K0W68_001845"
                     /pseudo
     CDS             complement(14966..17154)
                     /locus_tag="K0W68_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(17363..17438)
                     /locus_tag="K0W68_001846"
     tRNA            complement(17363..17438)
                     /locus_tag="K0W68_001846"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17403..17405),aa:Ala,seq:ggc)
     gene            complement(17478..17553)
                     /locus_tag="K0W68_001847"
     tRNA            complement(17478..17553)
                     /locus_tag="K0W68_001847"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17518..17520),aa:Ala,seq:ggc)
     gene            17774..18133
                     /locus_tag="K0W68_001848"
     CDS             17774..18133
                     /locus_tag="K0W68_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBI3073419.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18135..18527
                     /locus_tag="K0W68_001849"
     CDS             18135..18527
                     /locus_tag="K0W68_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBI3073420.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18579..19994)
                     /gene="gltX"
                     /locus_tag="K0W68_001850"
     CDS             complement(18579..19994)
                     /gene="gltX"
                     /locus_tag="K0W68_001850"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBI3073421.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20253..20328
                     /locus_tag="K0W68_001851"
     tRNA            20253..20328
                     /locus_tag="K0W68_001851"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20286..20288,aa:Val,seq:tac)
     gene            20373..20448
                     /locus_tag="K0W68_001852"
     tRNA            20373..20448
                     /locus_tag="K0W68_001852"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20406..20408,aa:Val,seq:tac)
     gene            20495..20570
                     /locus_tag="K0W68_001853"
     tRNA            20495..20570
                     /locus_tag="K0W68_001853"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20528..20530,aa:Val,seq:tac)
     gene            20575..20650
                     /locus_tag="K0W68_001854"
     tRNA            20575..20650
                     /locus_tag="K0W68_001854"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20608..20610,aa:Lys,seq:ttt)
     gene            20681..20806
                     /locus_tag="K0W68_001855"
     ncRNA           20681..20806
                     /ncRNA_class="other"
                     /locus_tag="K0W68_001855"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            20926..21267
                     /locus_tag="K0W68_001856"
     CDS             20926..21267
                     /locus_tag="K0W68_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HBI3073422.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(21258..22184)
                     /locus_tag="K0W68_001857"
     CDS             complement(21258..22184)
                     /locus_tag="K0W68_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3073423.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            22274..23272
                     /locus_tag="K0W68_001858"
     CDS             22274..23272
                     /locus_tag="K0W68_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBI3073424.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23269..23487)
                     /locus_tag="K0W68_001859"
     CDS             complement(23269..23487)
                     /locus_tag="K0W68_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBI3073425.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23489..25504)
                     /gene="ligA"
                     /locus_tag="K0W68_001860"
     CDS             complement(23489..25504)
                     /gene="ligA"
                     /locus_tag="K0W68_001860"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBI3073426.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(25575..26561)
                     /gene="zipA"
                     /locus_tag="K0W68_001861"
     CDS             complement(25575..26561)
                     /gene="zipA"
                     /locus_tag="K0W68_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBI3073427.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            26791..27552
                     /gene="cysZ"
                     /locus_tag="K0W68_001862"
     CDS             26791..27552
                     /gene="cysZ"
                     /locus_tag="K0W68_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBI3073428.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            27737..28708
                     /gene="cysK"
                     /locus_tag="K0W68_001863"
     CDS             27737..28708
                     /gene="cysK"
                     /locus_tag="K0W68_001863"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBI3073429.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29092..29349
                     /gene="ptsH"
                     /locus_tag="K0W68_001864"
     CDS             29092..29349
                     /gene="ptsH"
                     /locus_tag="K0W68_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBI3073430.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29394..31121
                     /gene="ptsI"
                     /locus_tag="K0W68_001865"
     CDS             29394..31121
                     /gene="ptsI"
                     /locus_tag="K0W68_001865"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBI3073431.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31162..31671
                     /gene="crr"
                     /locus_tag="K0W68_001866"
     CDS             31162..31671
                     /gene="crr"
                     /locus_tag="K0W68_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBI3073432.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         7821 a         8355 c         7880 g         7643 t
ORIGIN      
        1 ttctggtgca aagcgttgag ggcgaaaaag aggccagcgc cgcgcggcag accaagctgg
       61 cgctggatat cgccaacaaa acgctaccgc tgtttcgcca tgtcaatagc gagtcattac
      121 gcaaggtctg cgaaattatc cgcgatgaca ttcacgccga tgcggtggcg attactaata
      181 ccgatcatgt gctggcctat gttggcgtgg gtgaacataa ctatcagaat ggcgatgact
      241 tcattagccc gactacccgt caggcgatga attacggaaa aatcatcatt aaaaacaatg
      301 atgaagccca ccgcacacca gagattcatt ccatgctggt gatcccattg tgggagaaag
      361 gggtcgtgac cggaacgctg aaaatttact actgccacgc gcatcagatc acctcgtcat
      421 tacaggaaat ggcggtcggt ctgtcgcaaa tcatctccac gcaactggag gtttcacgcg
      481 ccgagcagct acgtgaaatg gcaaataagg cagagcttcg cgccctgcaa agcaaaatta
      541 atccccattt tctgtttaac gctctgaacg ctatttcatc gtcaatccgt ctgaatccgg
      601 ataccgctcg ccagttgatc tttaatctgt cgcgttatct gcgctataac attgaattaa
      661 aagacgatga gcaaatcgat atcaaaaaag agctgtatca aattaaagac tatattgcca
      721 ttgagcaggc ccgctttggt gacaagctga cggttatcta tgatattgat gaagaggtga
      781 attgctgcat tcccagcctg ctgatccagc cgttggtgga gaacgccatt gtccacggta
      841 ttcagccttg caaaggtaaa ggcgttgtca ccatcagcgt tgcagagtgc ggaaatcggg
      901 tacgcattgc ggtgcgagat accgggcacg gcatcgatcc aaaggtgatt gagcgggtcg
      961 aagcgaatga aatgccgggc aataaaattg gcctgctgaa tgtccatcat cgcgtgaagt
     1021 tattgtatgg cgaggggctg catatccgcc gcctggagcc ggggacggaa attgcgtttt
     1081 acattcctaa ccaacgcacc ccagtcgcct cacaggctac gttattgctt tgagccggag
     1141 tgatattgtg aaagtcatca ttgttgaaga cgaattcctg gcacaacagg aactgagctg
     1201 gctaattaaa gagcacagcc agatggagat tgtcggcacc tttgacgacg gtctggacgt
     1261 gttgaagttt ttgcagcata accgcgtcga cgccattttt ctggatatca atattccgtc
     1321 gctggatggc gtgttgctgg cgcaaaacat cagccagttc gcccataaac cgtttattgt
     1381 gttcatcacc gcgtggaaag aacatgcggt agaagcgttt gaactggagg cgtttgacta
     1441 cattctcaaa ccgtatcagg agtcacgtat taccgggatg ctgcaaaaac tggaagcggc
     1501 ctggcaacaa cagcagacca gcagtacgcc tgccgcgacg gtaacgcgtg agaatgacac
     1561 cattaatctg gtgaaagatg agcgaataat cgtcacgcca attaacgata tctattacgc
     1621 cgaagcgcac gagaaaatga cctttgtcta tacgcggcgt gaatcctacg taatgccgat
     1681 gaacattacc gaattttgca gcaaactgcc gccgtcgcat tttttccgct gccatcgctc
     1741 attttgtgtc aatctgaaca aaatacgcga aatcgaaccg tggtttaata acacctacat
     1801 tctgcgactg aaagatctgg attttgaagt gccggtcagc cgcagcaaag tgaaagaatt
     1861 tcgccagtta atgcatcttt aatcgggaac tttcatgaaa gcaccaggct tgcccgccga
     1921 tcaacaattt ttcgccgatc tgttcagcgg cctggtgctt aacccgcaac tactggggcg
     1981 cgtctggttt gccagccagc ctgcctcgtt accggtgggc agtttatgta ttgattttcc
     2041 ccgtctggat atcgtgctgc gcggcgaata cggcaacctg ctggaagcaa agcagcagcg
     2101 aatggtggaa ggagaaatgc tgtttattcc ggcacgcgcg gctaatttac cgatcaacaa
     2161 caaaccggtg atgctgttaa gcctggtgtt cgcaccgacc tggcttgggt tatcgtttta
     2221 cgatagccgc accacgtcgt tgttgcatcc tgctcgccag atccagcttc ccagcctgca
     2281 acgcggtgaa ggtgaagcga tgcttaccgc cctcacccat cttagccgtt cgccgctgga
     2341 gcagaatatc attcagccac tggtgttaag tttgctgcat ctttgccgca acgtggtgaa
     2401 tatgccgccg ggtaattcgc aaccgcgcgg cgattttctc tatcacagca tttgtaactg
     2461 ggttcaggat aattatgccc agccgctcac ccgcgagagc gtggcgcagt tttttaatat
     2521 cacgcccaat catctgtcaa aactgtttgc tcagcatgga acgatgcgtt ttatcgagta
     2581 tgtgcgttgg gtgcgaatgg cgaaggcgag gatgattttg cagaaatatc atctgtcaat
     2641 tcatgaagtg gcacagcgtt gcggttttcc ggatagcgac tatttttgtc gcgttttccg
     2701 gcgtcagttt ggtctgacgc cgggagagta cagcgcccgt tttcagggct aacgtcagaa
     2761 ggttaattct gtttccagca gcgtcaggat actttgtgca tcttgcgcgg caaacaacga
     2821 ctggcggaag tttttattca ccagtttacg cgccagctgc gagaagactt tcacatgatt
     2881 catcccttcg ttagcaccca gcgtcagcat gatcaccagt tcaacttcgc ccatttctga
     2941 ctgccagtca accggtttcg ccagccgggc aatgctgata ctggaatgac ggatccactg
     3001 agatttggtg tgcgggatcg ctacgccaaa accaacgccg gtggtaacaa tctcttcccg
     3061 ctgccagaca tcttcttcca gctcaaacgg atgttcagtg cgcccgttaa cgccgaggtt
     3121 gccgcacagg aactggatcg cctgctcttt attgctaaaa tcctgatcaa caaagatatt
     3181 ctccagcgcc agcagtgggc gaacgtcttc ttccggcgta aaggcggtga gtaacgcttc
     3241 aatttcctgc gcactgcggc attcacatgc ctgacgcgcc agttcccgac acgcctcgct
     3301 atccagttga cgaagctggc ttttcaccgc cggaatacgc gggctactca tactcagctc
     3361 atccaggccc agcccaagca gtagcggcag ataacggctt tcaccgccca gttcaccgca
     3421 aatgcctacc catttgcccc gctgatgcgc agtggtaact atttgctgca acatgcgcag
     3481 gaacgatggc gtaatcgggt tatatagcgg cgatacgcgc gggttattac gatcgaccgc
     3541 atacagatac tgggtcatat cgttggagcc gatactgaag aaatcgacct catcgcagaa
     3601 gtggtcgatg atgtagcaca ccgacggcac ttccaccatg atcccaagcg taatcgtctc
     3661 tgcatgacgc aggccatcgc gcttaagctc aacgatcgct ttttggatct cgcctttcac
     3721 ccataagatc tgatcgaggc tgttaaccat cgggatcatc aactgggcgt tgccgaaact
     3781 ggcggcgcgc aaaatggccc gcagttgagt gcggaacagg ccagcaaatt ccgggtaaat
     3841 acgtaccgcg cgatagccga ggaacgggtt ctcttcctgg ggaatattca gataaggaat
     3901 gcttttatcg ccgccgatgt ccatcgtgcg gaagataatc ggcttgtcgc ccgccgccag
     3961 tagcacctgc tggtaggctt caaattgctc ctgctcgtcc ggcgcgctgt cgcgatccat
     4021 atagagcatt tcggtacgga acagcccgac accttccgcg ccgttggcaa acgcgcctgg
     4081 cgcttccaga gcggtgccga tattcgccgc aatatcaata cgcttgttat cacgggaata
     4141 ggccagctgc gcggcagcct gcgcctgttg tttttggcgt ttatccgcca gcgtctgcgc
     4201 gacctgataa taaccgctca ccgcgtcatt cgggttaatc gccagcacgc cgcactgggc
     4261 gtcaagcact gcaggttgcc cggcataacg ggcaatcgca tccagcggca agccactcag
     4321 taccgggatc gccgaggcac gggccagaat cagtgtatgc gaggtgcgcc cggttttctc
     4381 cagaatcatg cccgcaagat ttttcaaatc aaggctcaaa aactggcttg gggttaaatc
     4441 ttcagccacc agaatggtcg gtttttccag caccagattg ttgcgcggct tcagttccgg
     4501 ctaggtgata tgcagcaact gttcgctgat atcgcgaatg tcgctgaccc gctcgcgcag
     4561 ataatcgctg gcagaagcag atagtttggc gcaaacctgc tccatattgc tgatgatcgc
     4621 cgcccccagt ccctgatgct gttctgtcat caggcgacgg atattgcctg caaattcatc
     4681 atcctgaatc agcgacaaat gagcgctgag gatagttttg ctttcgccgt cacgctcacg
     4741 caattgctgg ttcagttgct cggcaagcgt tgccaggctg tgctccagcc gggtggaatc
     4801 ttgcgcactg gcggggattg cccgataact gtcgaggctg tcgctttgta acagggtcag
     4861 cgtacccacg ccgacgccgc ttgccagcac attgccgtac agtaaatccg ggttaaggcg
     4921 gcttaatgaa cgcggcagcg gatgcgccgt cagttctgcc tgcgtcggct gaacgctgtc
     4981 gctgtcgata aagcgcacca ggatgtactc ttccagcacg cgccgcgcct gctcttcatc
     5041 gctgccgcta atgttcaggc tgcaactgtc attaaataag gtgccagttc caatcaacgc
     5101 cagcgagctt ttagcatcgg cctttgcgtt ctggcgatgg ttgataaagg tgatttcgct
     5161 ttgccactgg ctgcactgtt ctttaagttc ccacgccgga cgagcatgta gaccgttagg
     5221 caaaggacag agaaattgaa tcgttaacat aaccgctccg ggttaatcag gagatcatct
     5281 gaaatccgtc agttgaacaa ccgtctcacg cgtaagacgt tgaataaggg cagataaaag
     5341 ttgctccatc tgcaaaatgt cgcggcaatc ggcaatcgat gccgcgcaat gtccgtggcg
     5401 ggtggcaggc cccattacca ctgtgggtac gccagtatcg gttaagtgca ccgctccacc
     5461 gtccgtgccg ccgttactga acatatccgc ctgcaacgga acgccaattt ctgccgacac
     5521 ggtttcaatc cacgcggtaa gttttggcgg cgcaatcagc gatttatcgc ttaacaccag
     5581 catcggcccg ttaccaatct gacgatggtt agccgcgcca taatcaaagt ttttcgccca
     5641 gcaggcggta tcaagcacaa tggcgacgtc cggcgacacc gcgcgggtag cagtttgccc
     5701 gccgcgtaat cccacctctt cgctggaact tgccaccagc cacacttccg caggcagttc
     5761 ggcgtcgtgc agctcacgta gtaacgtcac cagcagatag caaccgaggc ggtcatcaaa
     5821 ggctttcccc atcacccgct ggtgagggag aacctgaaaa gctgtatcaa aggtgacgcg
     5881 atcgcctgga cgaattcccg cctgcatcac ttcgtcatac gagcgcgcac caatgtccac
     5941 gcgcatggcg ctgacgtcat tcccctgccg gtcgccgtca agcaggcctg gaattttgca
     6001 ctcttcacgg gtggtgatgc gcaccggctg cagctggcgg gcagccatgc gcacgttgcc
     6061 aaccggcagc acatcaattg ccccttcgcg ggagatgctg cgcaccataa atcccacttc
     6121 gtccatatgc gcacagatca tcaccttcgg acctgtcgat tcattgaggc ggatcagcac
     6181 cgatcccaga ccatcaaatc gtacttcttt ttgcaggcga tccgcttctt ccagtaggat
     6241 ctgccgcact tcctgttccg aggaggcgat cgcatctgcc tcgctcaacg cttttaatag
     6301 cgataaatcc attatgcctc tcccgtgagc aacactgttt tcggcatggc gtagagcact
     6361 tctgcgcctt gcggggtaac cagcacaaca tcttcgatgc gcacgccccc ttgccctggc
     6421 aaataaatcc ccggctccac ggtcagtaac atgcctggct gtagcgtcgt ggtatcccgc
     6481 ggtgaaaagc gtggatcttc gtgtacttca ataccgatag catgaccggt gttgtgacca
     6541 aaatagtcgc caaatcctgc ctcggtaatc acccggcgcg cggcgtcgtc aacctgttag
     6601 cagcgtacgc cagggcgaat cgcggagatt gccgccagct gtgcttgcag gacaatctga
     6661 tagacgttaa acagcgggtg agattcggca attacccctt cgccattcac cagcaaggtg
     6721 cgcgtcatat cagagcagta cccctgatag agcgcaccaa aatcaagagt gacaaactcg
     6781 cctgctgcaa caatcttgtc gctggctttg ccgtgcggca gcgccccacg ccagccgctg
     6841 gcgacaatgg tgtcaaaaga ggctttttct gcgccctgct ggcgcataaa ccactccagt
     6901 tcagcggcta tctcgcgctc gctcatcccc gcctgaataa agcggcgaat atgctccgct
     6961 ccgcgatcgg caatcccaca ggcgaggcgg attatctcca cctcctctgg cgttttgatt
     7021 tgccgcagca catccggcgt ggcgctaacc agtttcgcat tgagttcaga cttccagcgg
     7081 tgcgcggttt cccaactcac ctgctggcct tcaaaaccga gcgtttgcaa ctgctcatcg
     7141 gcaatgattt gattgacgat agtggtaagc gtgttcgtcg cgtcaagcaa atgcagctgg
     7201 tagccttgcg cacgggcttc cacctcaacg aaatagcgcg aatctaccag aatgtgcgca
     7261 ctttcacggc taataaccac atagcctgat ccggtggaga tccccagatg cggctgtttg
     7321 ttctgccgtg aggagagaag cactgcatcc agttgttgcg ccttaagcca gtcgcgcagc
     7381 gaagcgagta atgtcatctg gattttcctt cttacaggct atcgattaac aatttacctt
     7441 tacggaacat catcagacgc aggaacacca ccatcagcgc agttatgact gcccccagcg
     7501 cgatccccgc catatagacg cccaggttag tgaccagcgg ccatgcccag atagcggatt
     7561 ccgggaacca ttgcactgcg cccagccaga cagcggcggt tgagccgaca atcgcgccga
     7621 ccatatacga cggaatggcg gtgatggggc tttccagcgc aaacggaatc gcaccctcac
     7681 taatccccat aaaggcaagg aacatcgcgg tcttaccctg cggataaagt tgagcgttga
     7741 acaggcgttt gccggttaaa cgacggtcga taatggtcgc cagacccaga cctatcggcg
     7801 gaataacgat agcgatagaa cgtgcagtga ccggtaatac gtggtcagtg gtaaagctga
     7861 aggcaacaaa acctgcggct ttgttgatcg ggccaccaag gtcgattgcg gtcgccgcgg
     7921 cgatccccat tgcgtacatc agcgcgccct tctcacctgc ggcagtcagc acggtacgga
     7981 taccgccgtt gatccagccg ccaaaggggg taatgacgta gtacatcgcc agcatgacaa
     8041 aaatggcgga aagaatcggt agcaaaaatg tggttttgaa cgctaacagg aaatccggta
     8101 actggatttt ctggttcatc cacttcacaa ggtaacccgc gacgatagaa ataatcagcg
     8161 caccaatgaa ggtggacggc accggcgaag aggtcgccca ttgcatagtg ctgggatcga
     8221 agttcagcag ttgcgtcggc tgggtagaca tcaagccgcc gataaaacca gccgggaacg
     8281 ccagtttgcc accgatagag ttcgccacaa aagcagcaaa catcggaatg gcaaaaccaa
     8341 acaacacgcc gccgaaggac tgcgacagcc aggcaaattt cagtaaggag aggtcgaaac
     8401 cggagaattt cccgctatta agagcatcca tgatgccgat ctcagcagga attttaagcc
     8461 agctataagc aatcagctgg ctgaaagcga ggatcacacc gcccataatt aaggtcggca
     8521 ccatgcgtga aatccctgac atcacatgct gcgggagttc accccagaag ctgcttttag
     8581 aggcctgcgg attcttaact gccgctgccg caccggatgc gccaggcaca acggttgcac
     8641 tgcgtttttt aatggccata acaattatcc ctgtcgaata ttactgttgt tcagaggcaa
     8701 tcatctcttc gatttctttg atgatgcccg cagcgttttt aattgcgtcc tgcaaagtga
     8761 tttcataaac gtcgcgtgat tcgaaacgct cattatcttc cggggtaact gccacggagt
     8821 ggatgatgat ggttgcttcg gcgatatcct gcgccgtcag gcgattctgg ataccgtccg
     8881 cgccctgggt ttcaattttc acttcataac ctgcttctac cgccgcttct tccagcgcct
     8941 gagcggccat aaaggtgtga gccaggccca ttgggcaggc acataaggca attagtttct
     9001 tactcatcgt taattcctcg ttcagtgata gttgacgagt gaaattatga acgtgcaaaa
     9061 caaatcgccg ttaccagaca aataatgcat ttactggaga attaatcccg ctgtacggaa
     9121 gtgtgacgca ggtcggcctt gtgaggggga cacaaagaaa aattgaatta actgtatgat
     9181 ttaaaagatt atcgggagag ttacctcccg atataacaga aaggatttac agaatgtgcc
     9241 ctaaggtctg gcgtaaatgt gcaccggaac cgagaagacc cggattgtca tggacgatga
     9301 gatacaccgg aatatcatgg acatattctt taaagcgccc tttatcttca aatgcggcac
     9361 ggaaaccaga ggctttgaag aactcaagga agcgcggcac gataccgcca gcaatataaa
     9421 ccccaccaaa ggttcccagc gttaatgcca gattgccgcc aaaacggccc ataatgacgc
     9481 aaaacagcga caacgcacgg cggcaatcgg tgcagctgtc agccagcgcg cgttcggtaa
     9541 tatcttttgg cttgagattt tctggcaggc ggttgtcggc tttaacaatt gcgcgataca
     9601 aattcaccag cccaggacca gaaagcacgc gttccgccga aacatgacca atttccgcac
     9661 gcaatatttc gaggataatg gcctcttctt cactattcgg cgcaaaatca acgtgaccgc
     9721 cttcgcccgg caagcttacc caacgcttat cgacatggac cagatgcgca accccaagcc
     9781 ctgttccggc accgtaaacc gcaataggtt taccttcgac cggttctgcg ccaccaaact
     9841 gaatcagatg ctcttttttc agcatcggga tcgccatcga tacagcggta aaatcgttaa
     9901 taatttccag atggctaaaa ccgagattct ttttcatttc ggcaattgag aacgcccagg
     9961 tatggttggt cattgccacc cagtcaccgg taattgggca agcgatggca atacagccgt
    10021 ctttcacctc gaccttatgt tcttcaagat aaacgcgaat gaccgcttcg aggctggggt
    10081 aatcaagccc tgaataggtc ttagcctgcg agatttcacc actggcaata tcacacagag
    10141 caagacgtgc gttggtgccg cccacatcac cgactaatgc atactttgtc attcttcaac
    10201 tgctccgcta aagtcaaaat aattctttct cacactgtaa atacctgggg gcataacaac
    10261 aacgccggaa aggcaggctc cctgtaaata tcgatctggg tcacacattt actttatcgt
    10321 ttcagcacca attgcagcga tgcctttttg caagctgggc aaactaagta tctgaccccg
    10381 cataaggaat agaacatgct ccatccgcga gccagaacca tgttgttatt atcgctcccc
    10441 gccgtggcaa ttgggattgc gtccagtctt attctgattg tggtgatgaa aatcgcctcg
    10501 gtattacaga atttgctctg gcaacgactg ccgggaactc tggggatagc ccaggattca
    10561 cctatctgga tcatcggtgt attaacgcta acgggtattg cggtggggtt ggttatccgt
    10621 ttcagccagg gtcatgccgg accagacccc gcctgtgaac cgctgatcgg cgcaccagtt
    10681 ccgccctctg cgctaccagg acttatcgta gcattaattc tcggtcttgc tggcggcgtc
    10741 agcctggggc cggaacatcc gatcatgacc gtcaatatcg cccttgccgt cgccattggc
    10801 gctcgtctgt taccgcgcgt caaccgaatg gagtggacta ttttagcctc tgccggaacc
    10861 atcggcgcgc tgtttggtac acctgttgcg gcggcgttga tattttcgca aaccttaaat
    10921 ggcagtagtg aagttccgct atgggatcgt ctttttgcgc cgttaatggc ggcagcagct
    10981 ggtgcactta ctaccggatt atttttccat ccccattttt cactgcccat tgcgcattac
    11041 ggacagatgg aaatgaccga tattctcagc ggtgcaattg tcgcggcgat tgccatcgca
    11101 gcagggatgg tcgccgtatg gtgcttacca cggttgcacg cgatgatgaa tcaaatgaaa
    11161 aatccggtgc tcgtgctggg tattggcgga tttattctcg gaattctggg ggttattggt
    11221 ggaccagttt cgctgtttaa agggctggat gagatgcagc agatggtggc aaatcaggct
    11281 ttcagcacca gcgattactt tttgctggcg gtaattaaac ttgccgccct ggtcgttgct
    11341 gccgccagtg gctttcgcgg tgggcgaatc ttcccggcag tgtttgtcgg cgtggcatta
    11401 gggttgatgc tgcatgagca cgttcccgcc gttccagcgg caataaccgt ttcctgcgct
    11461 attctcggca tcgtgctggt ggtaacacgc gatggctggt taagtctttt tatggcggca
    11521 gtcgttgtac ccaataccac attgctaccg ctgctctgta tcgtcatgct tccggcatgg
    11581 ctgttattag caggtaagcc gatgatgatg gtcaatcgtc cgaagcaaca gccaccccac
    11641 gataatgttt agcaaaaaat gctctccttt atgatttaag agttatggcg atgatacggg
    11701 gctttacctc cccgtaatat tgcgttaaca ggccgctgac ggcttcgatc gtgaaggaga
    11761 ataacaatgt tcaggtcact gtttctggcg gccgccctga tggcatttac cccgcttgca
    11821 gcaaatgcag gtgaaatcac cctactgcca tcgatcaaat tacaaattgg cgatcgcgat
    11881 cattacggta attactggga cggtggtcac tggcgagatc gtgattactg gcatcgcaat
    11941 tatgaatggc gcaaaaaccg ctggtggcgt catgataatg gctatcatcg tggctgggat
    12001 aagcgtaaag catatgagcg tggctatcgt gaaggctggc gcgatcgtga cgatcatcgc
    12061 ggaaaaggcc gcggacatgg gcaccgccat taaacgcgtc ctccaatgga gcacaatgcc
    12121 tgatgcgacg ctcattcaac tacaatccca gcgccgtccc caccaacaac cagatattca
    12181 gcgccacgac cagcactaca atcacccagc ctgtctgttt tacgcgtttg ctgttcacca
    12241 gatcgcccat caacttgctg tcactggtga aaatcagcag tggaaccagc gccagggcga
    12301 taccaaaact taacagcacc tgactcataa ccagaatccg tgtcggatct aatcccatca
    12361 gaatgacaat aaatgacggc aacatggtga ctgtacgacg cacccacagc gggatatgga
    12421 agcgaatgaa tccctgcatc accacctgcc ccgccagtgt ccccaccacc gttgaggaca
    12481 gtccggcagc aaccagactt aacccaaaga ccgttgccgc agcatggctt aacagcggtt
    12541 gcagcgtcag ataagcctca tcaagatcgg caacaccagt atgaccagaa aagtggaacg
    12601 ccgccgcagc tgtagccatc atcgccagat tgacaaaacc ggcaatcgtc atggcgatag
    12661 ccacatccca tttggtggcg gaataacgtt gttgacgcga accgccatgt aaatgctgag
    12721 tgagcgaaga gtgcaaataa atcacatgcg gcataatcgt cgcccctaac acgcctgctg
    12781 ccaggaagac cgcctccgaa gtaggtaaac tcgggatcac cattccttta cccagctgcg
    12841 ccaggttagg ctgggagaaa atcaactcga caatgtaagc cgcggcaaca aacaacagta
    12901 acccgccaat cactttctcc agtggttttt gcccgcgacg ttgcagcatt aaaatcagga
    12961 aagtcgcgat ccccgtcagc accgcgccct gcaacaacga gacaccaaga atgagtttaa
    13021 aaccgatcgc cgcaccaata aattccgcca ggtcggttgc catcgcaata atttctgcct
    13081 gaacccaata gaaccacact acgggacgcg gatagtgatc acgaatctgc tccgccagat
    13141 tttttccggt ggcaatccct agtttggcag agaggatctg aatcagcatc gccatcaggt
    13201 tggcccaaac gacaacccac agtagctgat agccgaagct ggcacccgcc tgaatattgg
    13261 tcgcaaagtt accgggatcg atataaccaa tcgccgcaat gaacgcaggt cccattaatg
    13321 cgagcctcat cttgcgcgcc gcccgtccgc tgctactctc aacgcgatag ttcgtcatct
    13381 tgtgcctcta aaacatagcc tttgctatgt ttcatgctat gataaacgag aatgattatc
    13441 aaattcattt aaatggattg tggtgatttc tctgatagac caggattatg actacgaaaa
    13501 gattgatgac ttgaatgtta tggttatgtt taatgttagc acatttacat aactttcagc
    13561 ttccatacac aacatagcag aaatgtatga cagatcacta tttttgaagc ctgtcacagg
    13621 acgtcattat agtgtgtgtc agatctcgtt ttccttaacc atgttacata gaatgtgcac
    13681 ggaaatttaa cctgcctcat atttggagca aatatggacc gcgtccttca ttttgtactg
    13741 gcacttgccg ttgttgcgat tctcgcactg ctggtaagca gcgaccgcaa aaaaattcgt
    13801 atccgttatg ttattcaact gcttgttatc gaagtgttac tggcgtggtt cttcctgaac
    13861 tccgacgttg gtttaggctt cgtgaaaggc ttctccgaaa tgttcgaaaa actgctcaga
    13921 tttgccaacg aagggactaa cttcgtcttt ggtagcatga atgatcaagg cctggcattc
    13981 ttcttcctga aagtgctgtg cccaatcgtc tttatctctg cactgatcgg tattctccag
    14041 cacattcgcg tgttgccggt gattatccgc gcaattggtt tcctgctctc caaagtcaac
    14101 ggtatgggca aactggaatc ctttaacgcc gtcagctccc tgattctggg tcagtctgaa
    14161 aactttattg cctataaaga tatcctcggc aaaatctccc gtaatcgtat gtacaccatg
    14221 gctgccacgg caatgtccac cgtgtcgatg tccatcgttg gtgcatacat gaccatgctg
    14281 gaaccgaaat acgtcgttgc tgcgctggta ctgaacatgt tcagcacctt tatcgtgctg
    14341 tcgctgatca atccttaccg tgttgatgcc agtgaagaaa acatccagat gtccaacctg
    14401 cacgaaggtc agagcttctt cgaaatgctg ggtgaataca ttctggcagg tttcaaagtt
    14461 gccattatcg ttgccgcgat gctgatcggc tttatcgccc tgatcgccgc gctgaacgca
    14521 ctgtttgcca ccgttactgg ctggtttggc tacagcatct ccttccaggg catcctgggc
    14581 tacatcttct atccgattgc atgggtgatg ggtgttcctt ccagtgaagc actgcaagtg
    14641 ggcagtatca tggcgaccaa actggtttcc aacgagttcg ttgcgatgat ggatctgcag
    14701 aaaattgctt ccacgctctc tccgcgtgct gaaggcatca tctctgtgtt cctggtttcc
    14761 ttcgctaact tctcttcaat cgggattatc gcaggtgcag ttaaaggcct gaatgaagag
    14821 caaggtaacg tggtttctcg cttcggtctg aaactggttt acggctctac cctggtgagt
    14881 gtgctgtctg cgtcaatcgc agcactggtg ctgtaaaacc atacataaaa aagccgggga
    14941 tcattccccg gcttttttac gcctgttaat cagctaatgg ctgcgggcga ccaatcaaat
    15001 acccttgcag atattgcacc ccgagcttat gcaatagcgc ctgctgctgt tgcgtctcga
    15061 caaactccgc gaccacactc aatgacttcg ctttcgccag atcggtaatt gatctcacaa
    15121 tcatcgcatc cagcgtattc gtgacaatat ctttcacaaa gacgccatca attttgatga
    15181 tatcagcctg caaacgcttt aaccgttcgt agttggcata tccggtgcca aagtcatcaa
    15241 tcgcaatccg gaaaccaaac ttatgcagct gctcgatgtt gtacatgctg ctttctgcgt
    15301 tagaaaacgc ctgctcctcg gtgatctcaa gaatgaccgc ctgcggggag atgtgataac
    15361 gcttaaacag acgaataatc cgcccggcaa tattcttttg cagcagcgtg agcggcatta
    15421 aattgactga aaagcgcggt ccttttttgt cgcaagggtg tgttgccagc cacttcaaca
    15481 aggattccag cacttgcaaa tcaaaacgcg cgctaaggtt aaactgagca ataaggggca
    15541 gaaacttatc cggggtcata atgccgccgt catatttcag tcgcgcgagg atctcatcat
    15601 aaccttcacc ctctttgttg cgaattggct gggcgtagag cagcaaatca ccctgatcta
    15661 acgccgtgcg aatggtattc agcaatagca cctgtttagt ggtctgcccg gaaaccatct
    15721 cctctctgct atccagcgcc agcacatgat gatgtgcgca ggattgctcc gccagccagc
    15781 ttaactgccc caacaagggt tgcagggttt cctgatttcc atcaaaacgc ccccaggcag
    15841 caccatagcc catatccagc ccggtattgt tccagtgaat ttgccgacta ttcaggatgt
    15901 taaccatatg ctggagtcgc ccttccgttt ccggcccgct cagcactaac agcagttcac
    15961 tacccggcaa ttgatataac ttttcgtttt cctgcatcaa cggcagcagc gtacggtaaa
    16021 ttgatcggat acagtgaacg cgcatcatta agccgtaatg acgactcata aactcaagat
    16081 tatcaatgcg caggcagcaa aactcttgcc cgcctcttgc tccggcgctt gttccaacgc
    16141 acggaagttg ggcaggagcg ttaacggatc ggtcagcgcc tgcaaatgcc agcggcgatt
    16201 aagccattca ctgcgatgat aaatgcgcac catatagagc aggcaaacgc taaaggaaat
    16261 cagcaccgca agaataaatg ccagcgaata ttcggtttcc accccttgca aaaagttctg
    16321 gttgtaattc agaaggcaaa gcgttgaaac agccctggtg agatttaaaa acggatagcg
    16381 aagcttaccg accccgaggg taaagatgat gaagaaaacg ggtaccaggt aaccggcaat
    16441 aaagtcgttt tcataaggtg tgcacaacag aagtagaagc acgcttaacg ctgccagcca
    16501 gcttaaggta aatgcgcgtt tctctttgcc caacgacggc gcgatatccc tgcgccacaa
    16561 tatctgcgca aagtggggac ttacaatcat gcgagtgaga tagtagaaaa gcatgttgta
    16621 aataagcaca gcggtgaaaa ggcttagcaa atcaacgacc gtgaaaatgg catccgcatc
    16681 gccgaaaaag gtggatatct ttagcgggaa atcaaagaag ctccccacaa gatacatgct
    16741 gcatttgatg ccgatcggcg tcaccaaacc aagccagaac agacgttgcc agacatatcg
    16801 gctggtcaat ccataacgcc aacgcgtccc cagctgccag cgtaatattg cgcaggcaca
    16861 caacacgaca aacgtctggc taccgagcat gaccgcagtc tgtaaaaaag agagatgaaa
    16921 gttccacaga ttggtgcaaa acatcccgca taaaacggga acgacaccgc gccagccaaa
    16981 gataaacaag attgacagca ttacgcacag tggcatccac gccagaaaga tataactgga
    17041 atggataatg gcaagtggcg aaataaaacg ggatagctga ataagtacca cggtgagcgt
    17101 aaacgctagt gtgaatatct tgatattttt tatcaggtta tgctccacaa acatgagtac
    17161 actactctca ctgatcgggg ttttcaggca cacaaataat aagtttattc gcatcgcgaa
    17221 gcaaacaggg ggccagcctg ttgccgattt ttcatccaat gatgctttca ccaggcggca
    17281 ctaacccgga cgtagactgt attacaaaag cggcaaaaag cagagacaaa aaacccccgc
    17341 tttgcagcga gggttggaaa tttggtggag ctaagcggga tcgaaccgct gacctcttgc
    17401 atgccatgca agcgctctcc cagctgagct atagccccac gatgcgttta cgtaccaaat
    17461 ttgctgggtg caaaatttgg tggagctaag cgggatcgaa ccgctgacct cttgcatgcc
    17521 atgcaagcgc tctcccagct gagctatagc cccgtcacgt aaagcttgtc gagttgacgg
    17581 gcggcatcat atgaattccg cccgaatgtg tcaacggcaa attgcaacgt gtagtttcaa
    17641 tcgctgaaaa atcaggcaaa tgaacaattt tggaaaaccg ctcgcattca gtagttattc
    17701 atgtcacggt ttcctgtaaa gtggtgttat aaaatgaact actaatagac ccacatacat
    17761 tcagggaatt gttatgttca aggagaggat gacaccagat gaacttgcca gactgaccgg
    17821 ctatagccgc cagaccatta ataaatgggt acgcaaggag ggctggacaa cgtcaccaaa
    17881 acctggcgtc cagggtggca aagcccgact ggttcacgtc aatgaacagg ttcgtgaata
    17941 tattcgcaat gctgaacgtc cagaaggtca gggagaagcg cctgcccttt ctggtgatgc
    18001 gccgcttgaa gtattgctgg tgacactggc gaaagagatg acgccagttg aacaaaaaca
    18061 gtttacatcc ttgcttctgc gggaagggat tatcggattg ttacaacgct tagggattcg
    18121 cgatagcaaa taatatgaaa agattacgca ataaaatgac caccgaagaa ctggctgaat
    18181 gcctcggtgt ggctaaacaa actgttaacc gttggatcag agaaaaaggc tggaaaacgg
    18241 aaaaatttcc cggcgtgaaa ggcggtcgtg ccagacttat tctggtcgat acgcaagttt
    18301 gcgagtttat tcagaacacg ccagccttcc ataacacacc aatgttgatg gaagctgaag
    18361 agagaattgc tgaatacgct cctggagcac gcgccccggc gtatcgacaa atcatcaacg
    18421 cgatagataa tatgacagac attgagcagg gaaaagtcgc gcaatttttg tcacgtgaag
    18481 gaattcgcaa tttcctcgcc cgtctcgata ttgacgaatc agcataaata aaaaacggca
    18541 ggatattatc tcctgccgtt tatcttttta cacgctaatt actgctgatt ttcgcgttca
    18601 gcaataaaat ccagcgcttt gttgatacgc tcgatactgc gggtcttacc aattgcgtga
    18661 acggtaacat ccagtgctgg agactgcccc gccccggtta cggcgacacg cagcggcata
    18721 ccaactttac ccatacccac ttccagctca tccgccgtcg cctgaatagc gtgatgaacg
    18781 ttttcagcgg tccagtcagt aatcgcggcc agtttgtcac gaaccacttc cagcggctga
    18841 cgcgctaccg gacgcagatg ttttttcgcc gcatcggcat cgaactcagc aaaatcttcg
    18901 tagaaataac ggcagctctg tgccatctct ttcagcgtct tgcagcgttc gcccagcagt
    18961 ttcaccagat cagccagctg cgggccgtta cgggtatcga tattttcctg ctcaatgtgc
    19021 cactgtaagt gagtagcaac atactccggc ggcagcgcgt taatgtagtg atggttcagc
    19081 cacagcagct tgtcggtgtt gaacgcactg gcagatttgc tgacggcatt caaagtgaag
    19141 tatttgatca tctcttcacg agtgaagatt tcctgatcgc cgtgggacca gcccagacgc
    19201 accagatagt tcagcagtgc ttctggcaaa taaccgtcat cacgatactg cattacgctg
    19261 actgccccgt gacgtttgga cagtttttta ccgtcatcgc cattgatcat agaaacgtgc
    19321 gcgtaaaccg gcaccggcgc tttcagggcc ttaagaatgt tgatctggcg cggcgtgttg
    19381 ttgatatggt cttcgccacg gataacgtgg gtgatttcca tatcccagtc atcgacaacc
    19441 acacagaagt tataggttgg ggaaccatcg gtacggcgga taataagatc gtccagttcc
    19501 tggttgctga actcgatcgg accacggatc tgatcgtcaa aaacaacaga accttcctgc
    19561 gggttagcaa aacgtacaac acacggttca tcatcagcat gatgctcatg gctgtggcgg
    19621 cagcgaccgt cataacgcgg cttctcacct ttcgccattt gctcttcgcg cagcgcttcc
    19681 aggcgctctt tagagcaata gcatttataa gcagtgccct cttccagcat ctgatcgatc
    19741 accgcgttgt agcgatcaaa acgtttggtc tggtagtacg gaccttcatc ccactccagg
    19801 ctcagccagt tcatgccatc cataatggct tcgatagctt ccggcgtgga acgctcaaga
    19861 tcggtgtctt caatacgcag cacgaactca ccgccgtggt tacgtgcaaa aagccaggag
    19921 taaagagcag tacgcgcgcc gccaacgtgc agatagcctg ttgggcttgg cgcgaagcga
    19981 gttttgattt tcatgaaatg gccttacgtt tagaaagatg ccgacaaccg gcaaatcctg
    20041 gaaaaattga gtgggcgata ttctatcact cccgcctgat tcctcaatgt agttcgggtt
    20101 tctaccttac gcattattgt tttcgttgac aaattgcgca acacggtctg ttttgcgatc
    20161 atcttgctta aatttacgac gaacgaacaa tttctttaga aaatgcgttg actcattttg
    20221 aactctccct ataatgcggc tccacacagc gggggtgatt agctcagctg ggagagcacc
    20281 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccaac tactttatgt
    20341 agtctccgcc gtgtagcaag aaattgagaa gtgggtgatt agctcagctg ggagagcacc
    20401 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccaat ttctcgccag
    20461 ctaaatttct tgtaaaaatg tgaagtaccg aagtgggtga ttagctcagc tgggagagca
    20521 cctcccttac aaggaggggg tcggcggttc gatcccgtca tcacccacca cttcgggtcg
    20581 ttagctcagt tggtagagca gttgactttt aatcaattgg tcgcaggttc gaatcctgca
    20641 cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt tgctatctgc gatactcaaa
    20701 gattcgaacc tgcagcaggt ttgagttgag cgcagcgaaa caacggagcc gctcgcggcg
    20761 acggcccgaa gggcgagcga agcgagtcat cctgcacgac ccaccaatgt aaaaaagcgc
    20821 cctaaaggcg cttttttgct atctgcgatt tgtaagattg atttttcttc tcaagttcct
    20881 tgttcttctg ccgatattac ctcgtacagt gacgaggact gaacaatggc cactatctct
    20941 tcaacttcaa ttccttccat acaaacacag agtagtaaca gggcatcaca aggtagcgat
    21001 gttgcttcaa aaatcgcgcg gataagtcag caaatcatca aactcactca acaaattaaa
    21061 gagattgtag acactagcgg cagtgctgaa gacaaacaaa aacaggccga attgattcaa
    21121 cagcaaatca ccctactgga aacacaactg gcgcagttgc agagacagca ggcagaaaag
    21181 gcgcaggaga aagagcagcg tttgtctctg aatgtatccc ttctaaatcc tgttgaaaat
    21241 acgactcata tcgatatcta tatctgatac aacggagccc cttcccgacc agccattagc
    21301 gcattgccct ggtctcgtac cacgtcccat aacgcttcag cggcggtgga aagtgaacgg
    21361 ttcttcctgc gcaccagcat caactggcgt tcaacaaccg gcgtaatgcg ctttaccacc
    21421 agtgggctac cttcaggcaa tggtagcgcc agtgcgggaa gaatactgat gccgatgcct
    21481 gcggctacca tcgggaacag cgtcgccgga tgtccaatct cctgcacaat attcgcctga
    21541 ataccattgc gcgccagcgc tgcgtcaatc agcggtcggc tgcctgacgc gtaatcctgc
    21601 aacaccaatt tcgctccctg taatgcctgc cagggtacat aatcttccac ggccaaagcg
    21661 ctatcgcgat ggcaaagcag aaagaaaggc tcggaaagaa tcgcttcaca ttgcagatcg
    21721 cccactgggc ctggatcgat gacgatgcaa aaatcgacat ctccctggcg aatactttcc
    21781 atcacccact gctgcggacg atcgtgcagg acaaactgaa tatctggata gcggcgatgg
    21841 ctttcagcaa tgcattgcgg aataagatgc gcggaaatgg tctggctggc agcgacccga
    21901 acttttccgc tcagttgttg ccccatacgc ccggtatcgc gcaacgtgct gttaagttca
    21961 tccagcagtc gctcaagacg caaagccaac tgctgccctg catctgtaag caccacttca
    22021 cgcgtggttc tgtccagcag gcgaacacca gtatgatttt ccagctcctt cacactgtga
    22081 cttactgccg actggctcaa gccgatacgc tctcctgcac gactaaaact tttctcctgc
    22141 gctactgtga cgaaaacctt taattgtttt aaagaataat tcatctgttt tcttcatgaa
    22201 tggatgcaat aaatcaattt tatttctcaa actggaagaa gcacaataga accatcgatc
    22261 atctggagtc tttatgaaac tttttcgtat cctcgatcct ttcaccttaa ccctgatcac
    22321 ggtggtgttg ctggcctctt tctttccggc cagaggcgat ttcgtcccct tctttgaaaa
    22381 tctgaccacc gcagctattg ccctgctgtt ctttatgcac ggcgcgaagt tgtcgcgtga
    22441 ggcgattatt gctggcggtg gtcactggcg actgcatttg tgggtaatgt gcagcacctt
    22501 cgtgctgttt ccgattctgg gtgtactgtt tgcctggtgg aaaccggtaa atgtcgaccc
    22561 gatgctctac tccggttttc tctacttgtg cattctcccg gctaccgtgc agtctgcaat
    22621 cgccttcacg tcaatggcgg gcggtaacgt cgcggcggcg gtttgttctg cgtcagcatc
    22681 cagcctgctg gggattttcc tttcaccatt gctagttggt ctggtgatga atgttcacgg
    22741 tgcagggggc agccttgagc aggtcggtaa aattatgctg caactgctgc tgccgtttgt
    22801 gttggggcat ctttcccggc cgtggattgg tgactgggtg tcgcgcaata aaaaatggat
    22861 tgcgaaaact gaccagacgt ccattctgtt ggtggtttat acagcgttca gcgaagccgt
    22921 cgttaatggt atctggcata aagttggctg gggatcattg ctgtttatcg tggtggtcag
    22981 ctgcgttctt ctggctatcg tgattgtagt taacgtcttt atggcacgcc gactgggctt
    23041 caataaggca gatgaaatta ctatcgtctt ttgtggttcg aaaaagagtc tggcaaatgg
    23101 catcccgatg gcaaacattc tgttccccac atcggtgatc ggtatgatgg tgctgcccct
    23161 gatgattttc catcagatcc aattgatggt ctgtgcggtg ctggcgcgtc gatacaaacg
    23221 ccagaccgaa cagttacagg cgcagcagga aagcagcgcc gataaagctt aaagcggacg
    23281 cttcaggggc tggaccagtt gcgtcagccc ctcggtttta atcagcagcg tgatttgcat
    23341 tagctcaccg agcttccctg ccgggaactc atctttgcgg gcaaaccaca gcagatactc
    23401 ctccggcagg tcgattaagc gacgcccttt gtatttgcca aacggcatta tcgtattggc
    23461 tatttcaatc agctgctctt tttccatctc agctacccag caaacgcagc atttccgctt
    23521 cgtcgatgac ttcaatgccc agttcctgcg ccttcgccag tttagatcct gcagcttcac
    23581 ccgctatcac cagatcggtt ttcttcgaca cgctgcccgc gactttcgcg cccagttcga
    23641 ccagtcgagc tttagcgtca tcacgcgaca tctggcttaa gctgcccgta agcaccacgg
    23701 ttttaccagc aaacgggctg tcaatctctt ccgcgttgat aacgatcggc gcaggccagt
    23761 gaacaccttc cgccaacagc tcgctgatga cattgcggtt gctttcttcg gcaaagaagt
    23821 tgtgaacgtg ggatgcaacg acaatgccaa catcaggcac cttttgcagc tcttcaatcg
    23881 aagcggcttc cagcacttcc agcgtgccga aatatgccgc cagacctgct gcggtggcct
    23941 cgccgacttc acggatgcca agtgcataga ggaagcgagc aaaggtggtt tctttcgctt
    24001 tttccagcgc gttaaccacg ttttgtgccg attttggccc catacgctcc agtccggtca
    24061 gtttgcctgc ggtgagtttg aacagatctg ccggagtgtg gacatattct ttttcaacca
    24121 gctgatcgat gattttgtcg cccattccgt caacatccat cgcacggcgg gaaacaaagt
    24181 gtttcagcga ctctttacgc tgcgcaccgc aaatcaggcc accggtacag cgggcaaccg
    24241 cttcaccttc cacacgctca acgtcagaac cacataccgg acaatgcgtc gggaatacaa
    24301 cctcacgggt atcttccggg cgttcagaaa gcacgacgtt aaccacctgc gggatcacgt
    24361 cgccagcgcg gcgaatcacc actttatcgc caatgcgtaa accaagacgt tcgatttcat
    24421 ccgcattgtg taaggttgcg ttactcacca gcacgccagc aacatggaca ggttccagac
    24481 gcgcaacagg cgtaatcgcg ccagtacgcc caacctgaaa ctcgacgtca cgcacaaagg
    24541 tcatctgctc ctgcgccggg aatttaaacg ctaccgccca gcgcggggca cgcgcgacaa
    24601 agccaagctg ctcctgctgt tccagtgagt tgaccttaat caccacgccg tcgatatcaa
    24661 agcccagcgt cgggcggtct tcttccactt tgtgatagaa cgccagcact tcttccgccg
    24721 attcacaaag cgttacccga tcgctgaccg gcaaccccca ctttttaaat tgcagtaaac
    24781 ggccaagatg agtatccggc agctcgccac cttccagaac accaacgcca tagcagaaaa
    24841 aagtgagcgg tcgcttcgct gtaatacgcg gatcaagctg acgcagtgaa ccagctgccg
    24901 cattacgtgg gttagcaaaa actttcccgc ccgtgcgtcg cgcatcttcg ttaatctttt
    24961 cgaaccccgc ctgcggcagg aacacttcac cacgcacttc cagacgcgcc gggatattct
    25021 ctccgtgcag cttcagcgga atggcgcgaa tagtacgcac attagacgtg atatcttccc
    25081 cggtggtgcc atcgccacgg gtcgcggcac tgactaaaac gccattttca tacagaatac
    25141 tgacggcaag gccatccagc ttcagctcac agcaccaggt gactttctcg ttgtttttca
    25201 gacggtcctg cacacgtttg ttgaaagcaa gaaagctttc ttcatcaaaa acgttatcca
    25261 gtgacagcat tggtacttca tggcgtatct ggctgaaagc cgccagcggc gcagcaccta
    25321 cacgttgggt aggcgaatca ggcgtaatca gttctggatg tttggtttcc agctcgcgca
    25381 gttcgcgcat cagcctgtcg tattcagcgt cgggaatttc cggcgcatcc atcacatgat
    25441 aaagatattc atgatggcga agcgtcgttc gcagttctgt cagttgttgt tcgattgatt
    25501 ccatatcgca ccatcaatgc taaaaacccc cgacaagcgg gggttcgaag aggagttaat
    25561 ttgccttaag tgtatcaggc gttggcgtct ttgacttcgc ggatgatgtc ctggtactcg
    25621 cgcaatttct gcggagtcat catacggcgc tgatcgtcaa gcacgacacc gcccacttca
    25681 tcggcaatat gctgcgcaga ttgcagcatc agcttgaagt tctgcagctc gtcaccgtaa
    25741 gacggtacct gcataaagat agtgacaccc ggagtagtga aatccttcat ttcaggatca
    25801 aaggttcccg gtttcaccat attcgccagg ctgaataacg ccgggccgct gccatccggg
    25861 ctaagatgac gatggtaaat attcatatcg ccaaaaatga agcccgcttg ttgaatgctg
    25921 ttaagaagca gttcaccgtt tagctcgcta ccgtgatgcg ccgcgacgtt catgataatc
    25981 accgcttctt tgcgcttcgg tttatccata actggagccg gttccgctac aggctcaggc
    26041 tgtggtgccg ctacgggttc tgcaggctgg aaagcctgtt gtgccggttg cggtgctgaa
    26101 tgcacaggct gcggcgctgg cgcgacctgt ggcgaaactg gctgctgcaa cggctgttca
    26161 ggctgcggct gataggcagg ctgctgcatc ggctgcgctg gacgcggagc atgttgcggc
    26221 ggtacctgcg cttcaggcgg ctgctggatc ggttgacgcg gctgcgcaga cgcataaggc
    26281 ggttggtact ggtgttgcgg cgacggacga gcagcctcat gctcctgagc gttagccggg
    26341 gcatgattca cgcggtgaac acgaacctca ccaacgccct catcatcttc gacatcctcg
    26401 tcataagaat cgtcgtcacg ttttgacttc attcgtttta atggccgatc gcggaacata
    26461 gaagatcgtt ctttacggct ggtccagaaa ccatgtacca gtaaagcgat tatggcgatc
    26521 gcgccaacaa tgattaatat cagacgcaaa tcctgcatca ttatattctc tgttgttcta
    26581 acaccttgcc accacggcaa acatttactc actaagagta tttgccgatt acctcaagtg
    26641 caagtgcact attaactttc acagcacaaa gatagatgaa atcgtgcttt ttgctgtttt
    26701 ttcgaacata tcctaactgt ccattgcgca attacccggt aaaatacgca gaattttcct
    26761 gggattggtc aaaaggagct catcctgatt atggtttcat cattcacatc tgccccacgc
    26821 agcggttttt actattttgc gcaaggctgg aagctcgtct cgcaacctgg gattcggcgt
    26881 ttcgttattt taccgctgct ggtcaatatt ttgttgatgg ggggcgcatt ctggtggctc
    26941 tttacacagc tcgatgtctg gatcccgact ctcatgagtt acgttccgga ctggctacaa
    27001 tggctgagtt atctgttgtg gcctctggct gtcatctctg tgctgttagt gtttggctat
    27061 ttcttctcca cgattgctaa ctggattgcc gctccgttta acggtttatt ggctgaacaa
    27121 ctggaagcac gattgactgg cgctacaccg ccagataccg ggattttcgg tatcatgaaa
    27181 gatgtgccgc gaatcatgaa acgcgaatgg caaaaatttg cctggtatct gccgcgcgca
    27241 attgtattac taattcttta cttcatccct ggtattgggc aaaccgtcgc gccggtactg
    27301 tggttcctgt ttagcgcctg gatgttagcc atccagtatt gcgattaccc cttcgataac
    27361 cacaaagtgc cgtttaaaga gatgcgcacc gccctgcgca cacgcaaaat caccaatatg
    27421 cagtttggtg ctttaaccag cctgtttacg atgatcccgc tgcttaatct gttcatcatg
    27481 cccgttgccg tttgtggcgc gacggcgatg tgggtcgatt gctatcgcga taaacacgcg
    27541 atgtggcggt aacaatctac cggttatttt gtaaaccgtt tgtgtgaaac aggggtggct
    27601 tatgccgccc cttattccat cttgcatgtc attatttccc ttctgtatat agatatgcta
    27661 aatccttact tccgcatatt ctctgagcgg gtatgctacc tgttgtatcc caatttcata
    27721 cagttaagga caggccatga gtaagatttt tgaagataac tcgctgacta tcggtcacac
    27781 gccgctggtt cgcctgaatc gcatcggtaa cggacgcatt ctggcgaagg tggaatctcg
    27841 taaccccagc ttcagcgtta agtgccgtat cggtgccaac atgatttggg atgccgaaaa
    27901 gcgcggcgtg ctgaaaccag gcgttgaact ggttgaaccg accagcggta ataccgggat
    27961 tgcactggcc tatgtagctg ccgctcgcgg ttacaaactc accctgacca tgccagaaac
    28021 catgagtatt gaacgccgca agctgctgaa agcgttaggt gcaaacctgg tgctgacgga
    28081 aggtgctaaa ggcatgaaag gcgcaatcca aaaagcagaa gaaattgtcg ccagcaatcc
    28141 agagaaatac ctgctgctgc aacaattcag caatccggca aaccctgaaa ttcacgaaaa
    28201 aaccaccggt ccggagatct gggaagatac cgacggtcag gttgatgtat ttattgctgg
    28261 cgttgggact ggcggtacgc tgactggcgt cagccgctac attaaaggca ccaaaggcaa
    28321 gaccgatctt atctctgtcg ccgttgagcc aaccgattct ccagttatcg cccaggcgct
    28381 ggcaggtgaa gagattaaac ctggcccgca taaaattcag ggtattggcg ctggttttat
    28441 cccggctaac ctcgatctca agctggtcga taaagtcatt ggcatcacca atgaagaagc
    28501 catttctacc gcccgtcgtc taatggaaga agaaggtatt cttgcaggta tctcttctgg
    28561 agcagctgtt gccgcggcgt tgaaactaca agaagatgaa agctttacca ataagaatat
    28621 tgtggttatt ctaccatcat cgggtgagcg ttatttaagc accgcattgt ttgccgatct
    28681 cttcactgag aaagaattgc aacagtaatg ccagcttgtt aaaaatgcgt aaaaaagcac
    28741 ctttttaggt gcttttttgt ggcctgcttc aaactttcgc ccctcctggc attgattcag
    28801 cctgtcggaa ctggtattta accagactaa ttattttgat gcgcgaaatt aatcgttaca
    28861 ggaaaagcca aagctgaatc gattttatga tttggttcaa ttcttccttt aacggcataa
    28921 tgtttaatga cgtacgaaac gtcagcggtc aacacccgcc agcaatggac tgtattgcgc
    28981 tcttcgtgcg tcgcgtctgt taaaaactgg cgctaacaat acaggctaaa gtcgaaccgc
    29041 caggctagac tttagttcca caacactaaa cctataagtt ggggaaatac aatgttccag
    29101 caagaagtta ccattaccgc tccgaacggt ctgcacaccc gccctgctgc ccagtttgta
    29161 aaagaagcta agggcttcac ttctgaaatt actgtgactt ccaacggcaa aagcgccagc
    29221 gcgaaaagcc tgtttaaact gcagactctg ggcctgactc aaggtaccgt tgtgactatc
    29281 tccgcagaag gcgaagacga gcagaaagcg gttgaacatc tggttaaact gatggcggaa
    29341 ctcgagtaat ttcccgggtt cttttaaaaa tcagtcacaa gtaaggtagg gttatgattt
    29401 caggcatttt agcatccccg ggtatcgctt tcggtaaagc tctgcttctg aaagaagacg
    29461 aaattgtcat tgaccggaaa aaaatttctg ccgaccaggt tgatcaggaa gttgaacgtt
    29521 ttctgagcgg tcgtgccaag gcatcagccc agctggaaac gatcaaaacg aaagctggtg
    29581 aaacgttcgg tgaagaaaaa gaagccatct ttgaagggca tattatgctg ctcgaagatg
    29641 aggagctgga gcaggaaatc atagccctga ttaaagataa acacatgaca gctgacgcag
    29701 ctgctcatga agttatcgaa ggtcaggctt ctgccctgga agagctggat gatgaatacc
    29761 tgaaagaacg tgcggctgac gtacgtgata tcggtaagcg cctgctgcgc aacatcctgg
    29821 gcctgaagat tatcgacctg agcgccattc aggatgaagt cattctggtt gccgctgacc
    29881 tgacgccgtc cgaaaccgca cagctgaacc tgaagaaggt gctgggtttc atcaccgacg
    29941 cgggtggccg tacttcccac acctctatca tggcgcgttc tctggaactg cctgctatcg
    30001 tgggtaccgg tagcgtcacc tctcaggtga aaaatgacga ctatctgatt ctggatgccg
    30061 taaataatca ggtttacgtc aatccaacca acgaagttat tgataaaatg cgcgctgttc
    30121 aggagcaagt ggcttctgaa aaagcagagc ttgctaaact gaaagatctg ccagctatta
    30181 cgctggacgg tcaccaggta gaagtatgcg ctaacattgg tacggttcgt gacgttgaag
    30241 gtgcagagcg taacggcgct gaaggcgttg gtctgtatcg tactgagttc ctgttcatgg
    30301 accgcgacgc gctgcccact gaagaagaac agtttgctgc ttacaaagca gtggctgaag
    30361 cgtgtggctc gcaggcggtt atcgttcgta ccatggacat cggcggcgac aaagagctgc
    30421 catacatgaa cttcccgaaa gaagagaacc cgttcctcgg ctggcgcgct atccgtatcg
    30481 cgatggatcg taaagagatc ctgcgcgatc agctccgcgc tatcctgcgt gcctcggctt
    30541 tcggtaaatt gcgcattatg ttcccgatga tcatctctgt tgaagaagtg cgtgcactgc
    30601 gcaaagagat cgaaatctac aaacaggaac tgcgcgacga aggtaaagcg tttgacgagt
    30661 caattgaaat cggcgtaatg gtggaaacac cggctgccgc aacaattgca cgtcatttag
    30721 ccaaagaagt tgatttcttt agtatcggca ccaatgattt aacgcagtac actctggcag
    30781 ttgaccgtgg taatgatatg atttcacacc tttaccagcc aatgtcaccg tccgtgctga
    30841 acttgatcaa gcaagttatt gatgcttctc atgctgaagg caaatggact ggcatgtgtg
    30901 gtgagcttgc tggcgatgaa cgtgctacac ttctgttgct ggggatgggt ctggacgaat
    30961 tctctatgag cgccatttct atcccgcgca ttaagaagat tatccgtaac acgaacttcg
    31021 aagatgcgaa ggtgttagca gagcaggctc ttgctcaacc gacaacggac gagttaatga
    31081 cgctggttaa caagttcatt gaagaaaaaa caatctgcta atccacgaga tgcggcccaa
    31141 tttactgctt aggagaagat catgggtttg ttcgataaac tgaaatctct ggtttccgac
    31201 gacaagaagg ataccggaac tattgagatc attgctccgc tctctggcga gatcgtcaat
    31261 atcgaagacg tgccggatgt cgtttttgcg gaaaaaatcg ttggtgatgg tattgctatc
    31321 aaaccaacgg gtaacaaaat ggtcgcgcct gtagacggta ccattggtaa aatctttgaa
    31381 accaatcacg cgttctctat cgaatctgat agcggcgttg aactgttcgt ccacttcggt
    31441 atcgacaccg ttgaactgaa aggcgaaggc ttcaagcgta ttgctgaaga aggtcagcgc
    31501 gtgaaagttg gcgatactgt cattgaattt gatctgccgc tgctggaaga gaaagccaag
    31561 tctaccctga ctccggttgt tatctccaac atggacgaaa tcaaagaact gatcaaactg
    31621 tccggtagcg taaccgtggg tgaaaccccg gttatccgca tcaagaagta attcttgccg
    31681 cagtgaaaaa tggcgccca
//
LOCUS       DAETQN010000041        30162 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.041, whole genome shotgun sequence.
ACCESSION   DAETQN010000041 DAETQN010000000
VERSION     DAETQN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.041"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="K0W68_001867"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="K0W68_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBI3073433.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="K0W68_001868"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="K0W68_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBI3073434.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="K0W68_001869"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="K0W68_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBI3073435.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="K0W68_001870"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="K0W68_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBI3073436.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="K0W68_001871"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="K0W68_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBI3073437.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="K0W68_001872"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="K0W68_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBI3073438.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="K0W68_001873"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="K0W68_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBI3073439.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="K0W68_001874"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="K0W68_001874"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBI3073440.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="K0W68_001875"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="K0W68_001875"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBI3073441.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="K0W68_001876"
     CDS             complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="K0W68_001876"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBI3073442.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="K0W68_001877"
     CDS             complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="K0W68_001877"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBI3073443.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="K0W68_001878"
     CDS             complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="K0W68_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBI3073444.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="K0W68_001879"
     CDS             complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="K0W68_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBI3073445.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="K0W68_001880"
     CDS             complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="K0W68_001880"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBI3073446.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="K0W68_001881"
     CDS             complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="K0W68_001881"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBI3073447.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18363..18463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18516..18992)
                     /gene="rsd"
                     /locus_tag="K0W68_001882"
     CDS             complement(18516..18992)
                     /gene="rsd"
                     /locus_tag="K0W68_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBI3073448.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19087..19860
                     /gene="nudC"
                     /locus_tag="K0W68_001883"
     CDS             19087..19860
                     /gene="nudC"
                     /locus_tag="K0W68_001883"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBI3073449.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19900..20964
                     /gene="hemE"
                     /locus_tag="K0W68_001884"
     CDS             19900..20964
                     /gene="hemE"
                     /locus_tag="K0W68_001884"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBI3073450.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20974..21645
                     /gene="nfi"
                     /locus_tag="K0W68_001885"
     CDS             20974..21645
                     /gene="nfi"
                     /locus_tag="K0W68_001885"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBI3073451.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21688..22278
                     /locus_tag="K0W68_001886"
     CDS             21688..22278
                     /locus_tag="K0W68_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBI3073452.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22465..22737
                     /gene="hupA"
                     /locus_tag="K0W68_001887"
     CDS             22465..22737
                     /gene="hupA"
                     /locus_tag="K0W68_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBI3073453.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22750..23445
                     /locus_tag="K0W68_001888"
     CDS             22750..23445
                     /locus_tag="K0W68_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBI3073454.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="K0W68_001889"
     CDS             complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="K0W68_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBI3073455.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24110..25486
                     /gene="zraS"
                     /locus_tag="K0W68_001890"
     CDS             24110..25486
                     /gene="zraS"
                     /locus_tag="K0W68_001890"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBI3073456.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25483..26808
                     /gene="zraR"
                     /locus_tag="K0W68_001891"
     CDS             25483..26808
                     /gene="zraR"
                     /locus_tag="K0W68_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBI3073457.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26805..28094)
                     /gene="purD"
                     /locus_tag="K0W68_001892"
     CDS             complement(26805..28094)
                     /gene="purD"
                     /locus_tag="K0W68_001892"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBI3073458.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28106..29695)
                     /gene="purH"
                     /locus_tag="K0W68_001893"
     CDS             complement(28106..29695)
                     /gene="purH"
                     /locus_tag="K0W68_001893"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBI3073459.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7058 a         7868 c         8310 g         6926 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac atataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgct tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggtaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaat ccctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttggtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggagctg gtgccggaac gcctgcgtgg tgaaactgca
     5101 tctttcgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ttggctaagc tgagccagtc tggtcacaag
     5341 cgtattgaaa cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ctgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggac
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc cgggtggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcatt acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctt tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaggtga ccgacggtgt tgtaactgac gaaattcact acctttctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtttg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtgtatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccactggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 ccagaccatt actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga
    12781 aacgaggaag aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa
    12841 acacaacgct ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta
    12901 cgttcttaca ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct
    12961 ttccataccg gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga
    13021 tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc
    13081 gttttcgaga aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac
    13141 ggtgattccc gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag
    13201 atggtttgca ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac
    13261 gggcgcaggc gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat
    13321 agcaaatgtt cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc
    13381 gcgccgaggc ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc
    13441 cagaagaagt cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat
    13501 gtctcctgat aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc
    13561 tccgccagcg gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga
    13621 tgacgacgaa agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga
    13681 tgttcaaagc ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc
    13741 gttttgcgct tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa
    13801 agattggaaa gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc
    13861 agacgctgcg cccgttgggc cagtggttcc agatagccac tggcggctgg cgataacagc
    13921 gccatcatat cgtcgcgggt gagttgcgag gcatttagcg cccgttctac gtcagcagcc
    13981 gttttgccgt tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg
    14041 aaggttttca tgccgatgcc tccagaaatc cagtcagcgg gctggtggca tgagcaaaat
    14101 gactgcggct gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa
    14161 atgccttcgc catgttgacg ggatcgtccg ctacggcaat cgccgtattc actaacaccg
    14221 cgtcggcccc catttccagg gcctgcgcgg catggctggg aacgccgatg ccagcatcga
    14281 caaccaccgg aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc
    14341 cctgattcga gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca
    14401 gacgtttaca caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta
    14461 ccagcatttc ggcggctttc agagtttcga tgggatcggg caacagccag cgggcgtcag
    14521 ggtgaatctc taattttaac cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga
    14581 aaatggcttc ttccgctgtt ttcgccccgg aagtatttgg cagcagggtc acacccgccg
    14641 cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca
    14701 gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga
    14761 atttccctgt gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct
    14821 caaccccctg caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc
    14881 gtccactgct cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt
    14941 tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga
    15001 tcgttaaaca ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa
    15061 cgccaggctg cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt
    15121 ctctataccg cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac
    15181 caccgggcca accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca
    15241 caggcagcgg taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa
    15301 tccgaccgcg ctggcggtga taagcggcgt gttgagtacc acgcaggcgg cattaatctc
    15361 ctggcgagtc gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc
    15421 ttttaacgcc tcacccgtta accgttgttg taatgctgtc agttgaatat cgggattcaa
    15481 ctgtgtcagt cgctgatggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa
    15541 gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt
    15601 cccgacgcca gcccccgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat
    15661 aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag
    15721 aatttggcgg ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca
    15781 gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga
    15841 tactgccgac acccgttgct atcaccgcag gcgcgcgcgc cagactgata ccgccaatcg
    15901 ccacggtggg ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg
    15961 gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg
    16021 ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca
    16081 ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga
    16141 cgccatacgc ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt
    16201 tatagcggcg gcccagcgca attgccgcca cgacatcggc ttccacctct tcattgcgcc
    16261 gatctttgat gcgtagctgg agggtacgta cgcctgcatc caacagacgt tcgatccact
    16321 gtacgctgtc caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct
    16381 gatacatcac gcttcctcct tacgcaggta gatttctccg cctctggcgc ggaagttctc
    16441 cgacatatcc gccattccca cttcaatggt ttgcgcggcg gcgtaatcac gtacttcctg
    16501 gctgattttc atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc
    16561 tgactcttgc ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag
    16621 attaaactgg tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat
    16681 ttgcgcgcct ggatgccctt tcgccaggtc agcggcgtgg acggcaatct tataggtgat
    16741 aagcccctgc ttaacatctt ctttattagg cagacccaga tgctcttttg gcgttacgta
    16801 acagagcatc gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg
    16861 gtcatagccc ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg
    16921 gcagtgctct aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg
    16981 gccttcaatc atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt
    17041 atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc
    17101 gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg
    17161 ttgatagagg aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc
    17221 gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc
    17281 cgcatggata gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa
    17341 cgcttcccag gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg
    17401 cactgtaccg atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata
    17461 gcgaccggtg gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt
    17521 ttccacttct tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt
    17581 taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg
    17641 gataatcgca cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg
    17701 cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg
    17761 ctcgcggccc atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg
    17821 gcgggcgtag tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa
    17881 gccgctaaaa cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga
    17941 actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt
    18001 tgccagccct tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc
    18061 gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag
    18121 cgtcgggcta agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat
    18181 ataaatgcgt tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc
    18241 ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtc
    18301 aaggacgtgg cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg
    18361 atagcagatt aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc
    18421 gaataaacgg tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg
    18481 cgttgaatgt aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg
    18541 gcgtcaagca ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg
    18601 ccgatgtcag ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc
    18661 aggctggaat cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc
    18721 ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata
    18781 ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt
    18841 tcgtttagcc tcatgtacga ttctttgcca ggcttaatgc caaccagatt gtagtaagcc
    18901 acgagcagat gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg
    18961 cgctccgtca ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa
    19021 actgctcagt gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca
    19081 agagctatgg atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa
    19141 caaaaattat ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt
    19201 gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttagtacaa
    19261 cagcagcggc gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg
    19321 tttcaactgg ccggacgagg cgtacaactg gcggagtttt accgatcgca taaatactgt
    19381 ggttactgcg ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat
    19441 tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat
    19501 gattcgatcc tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc
    19561 ggattcgtcg aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag
    19621 agcggaatta aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctcag
    19681 tctttaatga ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa
    19741 gaattgctcg aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc
    19801 accgtagcgc gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga
    19861 tgatacactg accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat
    19921 cgttatctgc gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc
    19981 caggcgggtc gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg
    20041 tcgctgtgca aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac
    20101 ccgctggatg cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta
    20161 gggctctatt ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc
    20221 gacgtcgata aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg
    20281 gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt ttccggcagc
    20341 ccgtggacgc tggcgaccta catggtggaa ggcggcagca gcaaagcgtt caccgtgatc
    20401 aaaaaaatga tgtatgccga tccgcaggcg ctgcacgctc tactcgataa actggcgaaa
    20461 agcgtcactt tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc
    20521 gacacctggg gcggtgtgct taccgggcgc gattatcaac agttctcgct ctattacatg
    20581 cataaaattg ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg
    20641 tttaccaaag gcggcggaca gtggctggaa gcgatggcag aaaccggttg cgatgcgttg
    20701 ggcctcgact ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg
    20761 ttgcagggta atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa
    20821 gtagcgacta tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac
    20881 ggcattcatc aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga
    20941 ctgtctgaac aatatcaccg ctaaggagtg attatggatc tcgcgtcatt acgcgctcaa
    21001 caaatcgaac tggcttcttc tgtgatccgc gaggatcgac tcgataaaga tccaccggat
    21061 ctgatcgccg gagtcgatgt cgggtttgag cagggcggag aagtgacgcg agcggcgatg
    21121 gtgctgctga aatatccctc gcttgagctg gtggagtata aagttgcccg catcgccacc
    21181 accatgcctt acattccagg ttttctttcc ttccgcgaat atcctgcgct gctggcagcg
    21241 tgggagatgc tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg gatctcgcat
    21301 cctcgccgtc ttggcgtcgc tagccatttt ggtttgatgg tggatgtgcc aacgatcggc
    21361 gtggcgaaaa aacggctctg cggtaaattc gaaccgctct ccagcgaacc gggcgcgctg
    21421 gccccactga tggataaagg cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt
    21481 aacccgttgt ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta
    21541 caacgctgca tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc
    21601 tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc cctaattcag gtaaactgcg
    21661 gataatttcc gtatttgaga actcaacatg ttacaaaacc caattcatct gcgtctggag
    21721 cgcctggaaa gctggcagca tgtcactttc atggcttgct tatgcgaacg catgtacccc
    21781 aattacgcca tgttctgcca gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt
    21841 ctcgatctca tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa
    21901 ctggagaaat ttgaagaagc gattccttca gccgacgatt tcgatctgta cggcgtttat
    21961 ccggcaatcg atgcctgcgt ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa
    22021 acgctcgaac acgcggtgga agtgagtaag acctccatca cgaccgttgc gatgctggaa
    22081 atgactcagg ctggtcgcga aatgagcgat gaagagctta aagaaaaccc agctgtagag
    22141 caagaatggg acattcagtg ggaaatattc cgacttttag ctgagtgcga agaacgcgat
    22201 atcgagctga taaaaggcct tagggcagac ctgcgtgagg cgggtgagag caatattggt
    22261 ataatttttc agcaataaga ccagaaaacg tgatttaacg cctgatttgt cgtacctgga
    22321 gtcttccctt tcgccccccg tctggtctac atttgggggg cgaaaaaaag tggctatcgg
    22381 tgcgtgtatg caggagagtg ctattctggc atttccgtcg cactcgatgc ttagcaagcg
    22441 ataaacacat tgtaaggata acttatgaac aagactcaac tgattgatgt aattgcagag
    22501 aaagcagaac tgtccaaaac tcaggctaaa gctgctctgg agtccactct ggctgcaatt
    22561 actgagtctc tgaaagaagg cgatgctgta caactggttg gtttcggtac cttcaaagtg
    22621 aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc
    22681 gcagctaacg taccggcatt tgtttctggc aaggcactga aagacgcagt taagtaagat
    22741 tgcgtggcag tgaacagttt taacgaaggg gtggtttcac cccttttgtc tttctggcgt
    22801 cgatcattga tgctggctgg cgctctgctt ctcactgcct gtagtcataa ctcttcactt
    22861 cctcccttta ccgccagtgg atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa
    22921 gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc
    22981 acgacgcgag agtatcgctg gcagggcgat aacctcacgc tcatcaatat caatgtttac
    23041 agcaaaccgc cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct gagctttatg
    23101 caacgtgaat ccgatgggga aaagcagcag ctttctaacg accaaatcga tttataccgt
    23161 tatcgtgctg atcagatccg ccagattagc gatgccttac gtcaggggag agtcgtgctg
    23221 cgccaggggc gctggcatgc gatggaacag accgtgacca cctgcgaagg gcaaaccatt
    23281 aaacctgatt tagattcgca ggcgatagcg catatcgagc gccgccagag ccgctcttct
    23341 gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt cgcaattact gttagtggca
    23401 aactctgatt tctgtcgctg gcaacccaac gagaaaacgt tctgatttac cagtggccca
    23461 tacccatatg accgccacca ccgcagccgc cgtagcccat gcccattccg gctccgcgcg
    23521 gaatacccgc ttcagccatc gcgatatctc gtttcacccg taactcatct aacgactgac
    23581 gcaaattctc catctctttg gcgaccgcgt taattttgct gctatccggt gggttcgcgg
    23641 ctaacagggc attgtattca taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt
    23701 gagcgtaaaa gtcattatgg attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg
    23761 cggcattttg ctgccacata ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg
    23821 ccattgctga aagcgccatc attaccaggg caattttcgt gttccgtttc atggttaatc
    23881 ctccagtggt tgtcttactt cgggtattgc atcttccgtg ccaacgatga aacgctgata
    23941 tgacgggtaa tctggcatga taaacgagta aaaatgactc gcctgctgcg ggtagcgagt
    24001 catttttact cattgaaact taagcctttg tgttacagcg cagggtaagc gctgataaaa
    24061 gatggcatga tttctgctgt cagaaaggga tgagcaggca aagaagaaga tgcgttttat
    24121 gcaacgttct aaagactcct tagctaaatg gttaagcgcg atcctccccg tggtcattgt
    24181 tgggctggta gggttgtttg cggtgacggt gattcgcgat tatgggcgcg agactgccgc
    24241 cgccagacaa acgctgctgg aaaaaggcag tgtacttatt cgcgctcttg aatccggctc
    24301 gcgcgtcggc atggggatgc gcatgcatca tgcgcagcag caggccttac tggaagaaat
    24361 ggccgggcag cctggagtac gttggtttgc ggtcacggat gaacaaggaa caatcgtgat
    24421 gcatagcaac tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt
    24481 acatccggga gatgaagaag cgtggcggcg gatcgatagc gcagacggcg agcctgttct
    24541 ggaaatttat cgccagtttc aaccgatgtt tgctgctgga atgcaccgga tgcgccatat
    24601 gcagcagtat gccgcgacac cacaagcaat tttcatcgct ttcgacgcca gtaatattgt
    24661 gagtgccgaa gatcgtgagc agagaaacac cctgattatc ctcttcgccc tggcgacggt
    24721 cttgctggca agcgtattgt cattcttctg gtatcgccgc tatttgcgct cgcgccagct
    24781 tctacaagat gaaatgaagc gcaaagagaa gctggtggcg ctggggcatc ttgcggcagg
    24841 cgttgcccac gaaatccgta acccactttc ctcgattaaa ggactggcga aatactttgc
    24901 cgagcgcgcg cctgcagggg gagaagcgca tcaactggcg caggtgatgg cgaaagaggc
    24961 cgaccgttta aaccgcgtgg taagcgagct gctggaactg gttaagccaa cgcatctggc
    25021 tttgcaggcg gtggatctca acacgctgat taaccactca ttacagctgg taagccagga
    25081 tgcgaacagc cgggagatcc agttacgctt taccgccaac gacacattac cggaaattca
    25141 ggccgatccg gacaggctga ctcaggtcct gttgaatctc tatctcaatg ctattcaggc
    25201 gattggtcag catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat
    25261 cagcgttacc gacagcggta agggaattgc ggcagatcag cttgatgcca tcttcactcc
    25321 gtacttcacc actaaagccg aaggcaccgg actggggctg gcggtcgtgc ataatattgt
    25381 tgaacaacac ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac
    25441 cctctggctt ccggtcacta ttacgcgtaa gggcccacaa ggatgacgca cgataatatc
    25501 gatattctgg tggtggatga tgacattagc cactgcacta ttttgcaggc tttactgcgc
    25561 ggctggggct ataacgtcgc gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg
    25621 gaacaggttt ttgatcttgt gctttgcgat gtgcgaatgg cggagatgga cggcattgcc
    25681 acgctaaaag agatcaaagc gttaaatccg gcgattccgg tgctgattat gaccgcatac
    25741 tccagcgtcg agacggcggt agaagcgttg aaaaccgggg cgctggatta tctcatcaag
    25801 ccgctggatt tcgataacct gcaggcgacg ctggaaaagg cgctcgcgca tacgcacagt
    25861 attgacgctg aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg
    25921 gcgatgcaac acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg
    25981 atccacggcg attccggcac cggtaaagag ctggtcgcca gggcgattca cgccagtagc
    26041 gcacgtagcg aaaaaccgct ggtaacgctc aactgtgcgg cactcaacga atccttgctg
    26101 gaatctgaat tgttcggtca cgaaaaaggg gcgttcactg gagccgatag acgccgggaa
    26161 gggcgctttg ttgaggcgga cggcggcacg ctgtttctcg atgaaattgg cgatatctcg
    26221 ccgatgatgc aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt
    26281 agcaaccaga ctatctcggt tgatgtccgg ttgattgcgg cgacccatcg cgatcttgcc
    26341 gcagaggtga atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg
    26401 attgaagtac catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tgtccatttt
    26461 cttcagcgct ttgccgagcg taatcgcaag gcggtaaaag gctttacgcc acaggcgatg
    26521 gatttgctga tccactacga ctggccggga aatattcgtg aactggaaaa cgcggtggaa
    26581 cgggcggtgg tgctgctgac cggggaatat atttccgaac gcgagctgcc gctggcgatt
    26641 gccggtacgc cgatcccact ggggcaaagt caggatattc agccgctggt ggaagtcgaa
    26701 aaagaagtga ttctggcggc actggagaaa acgggcggca acaaaaccga agccgcccgt
    26761 cagttaggga tcacgcgcaa aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt
    26821 tcgatagcgc gccagccgat atctttccgg cagaagcagt cgtcccagtg aatatcggtc
    26881 attaaggcat aagcgcgttt ctgcgcttct gccacggtat gacccagcgc ggtgacgcac
    26941 agtacgcgcc cgccgctggt cactacctgc tcgttatccg ccagtttcgt gcccgcgtgg
    27001 aacactttgc cgcctgccac ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg
    27061 taatcgcccg gatatccacc cgcagccatc accacgccga gagaagcacg ctcatcccac
    27121 tctgacgttt tctcgtccag tttgccttca caggccgcca ggcagagctc aaccagatcg
    27181 gacttcatgc gcagcataat cggctgggtt tctggatcgc caaagcggca gttaaattcg
    27241 ataaccttcg gattgccctg tttgtcgatc atcaggcccg cgtagagaaa accggtgtag
    27301 gtgttgcctt ccgccgccat gcctttcacg gttggccaga tgatacgttc catggtgcgc
    27361 tgatgaactt catcggttac taccggcgcg ggggagtaag cgcccatccc gcccgtgttt
    27421 ggtccggtat ctttatcgcc tacgcgtttg tgatcctggc tggtggccat cggcagcaca
    27481 tgctcgccgt ctaccatcac gataaagctc gcttcttcgc cgtcgaggaa ctcttcgata
    27541 acgatgcgat gacccgcgtc gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct
    27601 tcggcttctt ccagcgtcat cgccacgata acgcctttcc cggctgccag accgtccgct
    27661 ttaatgacga tcggcgcacc tttctcacgc agatacgcca gcgcaggttc tacctcggtg
    27721 aagttctggt attccgctgt agggatctta tggcgggcca ggaaatcttt ggtaaacgct
    27781 ttcgaacctt ccagttgcgc ggcacctgcg gttgggccga agattttcat cccggcggcg
    27841 cggaaggtat cgaccacgcc tttcaccagc ggcgcttccg ggccgacgat ggtcagatca
    27901 atcttttcgt tttgtgcgaa atccaacagc gccgggatat cggtcacgcc gatagcaacg
    27961 ttttgcagcg cgggttccag cgcagtgcct gcattacccg gagcaacaaa aacagtttca
    28021 accagcggcg actgcgccgc tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc
    28081 actaatactt tcatctattg ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca
    28141 tcgcaatacc gtgctcgtcg gcggcggcaa tcacttcgtc atcacggata gaaccgccag
    28201 gctggattac gcaggtcacg cctgcagcgg cggcggcatc aataccgtcg cggaacggga
    28261 agaacgcgtc agaagccatc gaggaacctt tcacttccaa gccttcatcg gcagctttaa
    28321 taccggcgat tttcgcggag tacacgcggc tcatctggcc cgcgccaatc ccgatggtca
    28381 tattgttttt ggcatagacg atagcgttgg atttcacgaa cttcgccact ttccagcaga
    28441 acagcgcatc acgcagttcc tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt
    28501 ccgcaccgac catgcccagg tcacgatcct gaaccagcag accgccgttc acgcgtttga
    28561 aatcaagacc cggaacacgc tcgccccact gaccgcaggt cagaacgcgt acgttctgct
    28621 tggcggcggt gattttcagg gcttcttcgc tggcggaagg cgcaataatc acttcgacaa
    28681 actgacgaga aatgatggcc tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga
    28741 tgccgccgaa tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg
    28801 aattgccgat agccacgccg caagggttgg cgtgcttcac aatcacacat gccggctcgg
    28861 cgaactcttt cacgcactcc agcgccgcat cggtatcggc gatgttgtta taagagaggg
    28921 ctttaccctg aacctgggtt gcggtagcaa cggaggcttc tttcacattc tcttctatat
    28981 agaaggcagc ctgctggtgg ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt
    29041 tcaggttcag cgtgcgtggg aagcgaccgg cggcttcttt gctttcaccg tggtaagccg
    29101 gaaccatgct gccgaagtag ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg
    29161 ctttgatggc gaggtcgaaa cgggttgcaa gcgtcagcga tccttcgttg tcatccatct
    29221 ctttaataat ggcgtcgtag tcgctgctct tcaccacgat tgcgacatct ttatggttct
    29281 tagcggcgga gcgcaccatg gtcgggccgc cgatatcgat gttctcaacc gcatcttcca
    29341 gcgagcagcc ttcacgagca acagtctggg cgaacggata caggttaaca accaccatat
    29401 cgataggctg gatctgatgt tcttccataa tggcatcgtc ctggccgcga cggcccagaa
    29461 tgccaccatg tactttcgga tgcagggtct tcacgcgtcc atccatcatc tccgggaaac
    29521 cggtgtaatc ggaaacttcg gttaccggca gacctttttc tgctaacaga cgggcagtgc
    29581 cccctgtaga cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac
    29641 cggctttgtc agaaacactg agcagagcgc ggcggactgg acgacgttgt tgcatggtaa
    29701 atcccctgga tttgactatt acagagagcg ttagctgaat ttttcgcgaa aaactcagct
    29761 aacgccccta acggggcatc cttatttttc gcccgcattg taacgaaaac gtttgcgcaa
    29821 cgctcgcgaa tttttctctt tcaatggtga tcacaatttt gacagtggtt accgtgggaa
    29881 aaatacagaa attacattga tgattgtgga taactctgtg cgtaaaaagg tataaagcgg
    29941 ggttttgctg gggaatgcag cagtcagtca tttttctgca atttttctat tgcggcctgc
    30001 ggagaaatcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    30061 agccggttcg gttgaagaga aaaatcctga aattcagggt tgactctgaa agaggaaagc
    30121 gtaatatacg ccacctcgcg acagtgcgct aaagcgcgtc gc
//
LOCUS       DAETQN010000042        30000 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.042, whole genome shotgun sequence.
ACCESSION   DAETQN010000042 DAETQN010000000
VERSION     DAETQN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.042"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            38..580
                     /locus_tag="K0W68_001894"
     CDS             38..580
                     /locus_tag="K0W68_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3073460.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /locus_tag="K0W68_001895"
     CDS             663..1364
                     /locus_tag="K0W68_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBI3073461.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /locus_tag="K0W68_001896"
                     /pseudo
     CDS             1389..3988
                     /locus_tag="K0W68_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /locus_tag="K0W68_001897"
     CDS             3979..5049
                     /locus_tag="K0W68_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3073462.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5061..5603
                     /locus_tag="K0W68_001898"
     CDS             5061..5603
                     /locus_tag="K0W68_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3073463.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5611..6126
                     /locus_tag="K0W68_001899"
     CDS             5611..6126
                     /locus_tag="K0W68_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI3073464.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6092..6828
                     /locus_tag="K0W68_001900"
                     /pseudo
     CDS             6092..6828
                     /locus_tag="K0W68_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            6939..7949
                     /gene="pyrD"
                     /locus_tag="K0W68_001901"
     CDS             6939..7949
                     /gene="pyrD"
                     /locus_tag="K0W68_001901"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBI3073465.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8123..8665
                     /gene="zapC"
                     /locus_tag="K0W68_001902"
     CDS             8123..8665
                     /gene="zapC"
                     /locus_tag="K0W68_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBI3073466.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8662..9771)
                     /gene="ycbX"
                     /locus_tag="K0W68_001903"
     CDS             complement(8662..9771)
                     /gene="ycbX"
                     /locus_tag="K0W68_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBI3073467.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10015..12123
                     /gene="rlmKL"
                     /locus_tag="K0W68_001904"
     CDS             10015..12123
                     /gene="rlmKL"
                     /locus_tag="K0W68_001904"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBI3073468.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12135..14042
                     /locus_tag="K0W68_001905"
     CDS             12135..14042
                     /locus_tag="K0W68_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3073469.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISHLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQMADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14172..15425
                     /gene="pqiA"
                     /locus_tag="K0W68_001906"
     CDS             14172..15425
                     /gene="pqiA"
                     /locus_tag="K0W68_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBI3073470.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15430..17070
                     /gene="pqiB"
                     /locus_tag="K0W68_001907"
     CDS             15430..17070
                     /gene="pqiB"
                     /locus_tag="K0W68_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBI3073471.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17067..17630
                     /gene="pqiC"
                     /locus_tag="K0W68_001908"
     CDS             17067..17630
                     /gene="pqiC"
                     /locus_tag="K0W68_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBI3073472.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17886..18053
                     /gene="rmf"
                     /locus_tag="K0W68_001909"
     CDS             17886..18053
                     /gene="rmf"
                     /locus_tag="K0W68_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBI3073473.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18123..18641)
                     /gene="fabA"
                     /locus_tag="K0W68_001910"
     CDS             complement(18123..18641)
                     /gene="fabA"
                     /locus_tag="K0W68_001910"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBI3073474.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18710..20470)
                     /locus_tag="K0W68_001911"
     CDS             complement(18710..20470)
                     /locus_tag="K0W68_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBI3073475.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20656..21108
                     /gene="matP"
                     /locus_tag="K0W68_001912"
     CDS             20656..21108
                     /gene="matP"
                     /locus_tag="K0W68_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBI3073476.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21184..22224)
                     /gene="ompA"
                     /locus_tag="K0W68_001913"
     CDS             complement(21184..22224)
                     /gene="ompA"
                     /locus_tag="K0W68_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBI3073477.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22579..23088)
                     /gene="sulA"
                     /locus_tag="K0W68_001914"
     CDS             complement(22579..23088)
                     /gene="sulA"
                     /locus_tag="K0W68_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBI3073478.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23307..23936
                     /gene="sxy"
                     /locus_tag="K0W68_001915"
     CDS             23307..23936
                     /gene="sxy"
                     /locus_tag="K0W68_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBI3073479.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23899..26061)
                     /gene="yccS"
                     /locus_tag="K0W68_001916"
     CDS             complement(23899..26061)
                     /gene="yccS"
                     /locus_tag="K0W68_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBI3073480.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26071..26517)
                     /locus_tag="K0W68_001917"
     CDS             complement(26071..26517)
                     /locus_tag="K0W68_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBI3073481.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26640..28694
                     /gene="helD"
                     /locus_tag="K0W68_001918"
     CDS             26640..28694
                     /gene="helD"
                     /locus_tag="K0W68_001918"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBI3073482.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISSQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28726..29184)
                     /gene="mgsA"
                     /locus_tag="K0W68_001919"
     CDS             complement(28726..29184)
                     /gene="mgsA"
                     /locus_tag="K0W68_001919"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBI3073483.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29280..29942)
                     /locus_tag="K0W68_001920"
     CDS             complement(29280..29942)
                     /locus_tag="K0W68_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBI3073484.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         7733 a         7084 c         7877 g         7306 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      781 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
      901 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
      961 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
     1021 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggg ggaaaggtag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca
     1321 atgattacgg ggcggaaacg ccacaactca actgtaaatc gtaagccgtc ttcagttaag
     1381 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1741 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1801 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1861 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1921 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     1981 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2041 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2101 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2161 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2221 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2281 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2341 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2401 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
     2461 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2521 aggagagcaa atttgcctag gccacgttgc agtggggcgg accgtgggga acgacatggt
     2581 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2641 ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc
     2701 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
     2761 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2821 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2881 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     2941 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3001 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3061 tctcgctggg gggttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3121 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3181 ataccaggaa aaaaattacg cctggatgac ctctaacacc agcatcgata acgaagggca
     3241 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     3361 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     3601 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcattattg atgatgatgg
     3841 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     4381 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     4441 ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgcatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaag aaacgatatt tcagtgcgtt atcttgttct
     5101 ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt atgcatattt
     5161 atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc
     5221 atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg
     5281 atttgaatat cgatatcacc ggttgtgccg ctagtattac tggcgcggac gtccttttta
     5341 gcggcgaggc tgacaccctt acgccgacac tgctcaaact aactgacaca ggcggaagcg
     5401 gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag caagaaatcc
     5461 cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca ctcaaatatc
     5521 aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg gcggttctct
     5581 attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg gattctgttc
     5641 ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt caacattagc
     5701 ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat caatgtgcag
     5761 ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga gaaatctttc
     5821 atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac cttttcagga
     5881 acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga tgcagccagc
     5941 gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa ccaggcgagt
     6001 aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca attgcgacct
     6061 gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt acttcattat
     6121 gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc tgctggtggc
     6181 gggtgaattg cgggcaggag tggtggtcgg cggaacgcga tttatctttc cggcagacag
     6241 agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta ttaacagtaa
     6301 aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag caccattact
     6361 ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc gcttgctgag
     6421 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     6481 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     6541 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     6601 ctggacacgg aattccaggg tgtacaactc actaacccaa cgccttacta cattaacctg
     6661 attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagttgtgcc gcctaaaagc
     6721 cagcgtcaga caagctggtg tcaggcaatc gcaccgtgtc atgtcgcctg gcgggcgata
     6781 aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa caggttcgga
     6841 aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccgaact cgcacataat
     6901 acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc ttcgttcgta
     6961 aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag caattacgcc
     7021 gtattacagg aacgccgttt gaagcactgg tgcggcagaa agtgcctgcg aaacctgtta
     7081 actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt gataaagacg
     7141 gggagtgcat tgacgcgtta ggcgcgatgg gatttggatc gatcgagatc ggtaccgtca
     7201 cgccacgtcc acagccaggt aatgacaagc cgcgtctctt tcgtctggta gatgccgaag
     7261 gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta gagaacgtaa
     7321 aaaaggccca ttatgacggc gtcctgggta ttaacatcgg caaaaataaa gatacgccag
     7381 tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc tatgcgggat
     7441 atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg caatatggtg
     7501 aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg caagcgatgc
     7561 accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa gaagaattga
     7621 tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca accaatacca
     7681 cactcgatcg ttctcttgtt cagggaatga aaaattgcga tcaaaccggt ggcttaagtg
     7741 gtcgtccgct tcagttaaaa agcaccgaaa ttattcgccg cttgtcactg gaattaaacg
     7801 gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg cgtgaaaaga
     7861 ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa ggtccgccgc
     7921 tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt tataagataa
     7981 tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt atttagacat
     8041 tttgtacttt tattgttgag gttattaagc gaagcgacaa tggattgtgt tgctgcggtt
     8101 ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc gttggtatta
     8161 cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat ttcgctcacg
     8221 ttttgcgcgc aagatgttga ctccagatgc cttttctccc gcaggctttt gcgttgacga
     8281 cgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat ctaaagaaca
     8341 aaaagccgaa ctggtgctga atgcactggt agcaattcgc tatctcaaac cgcaaatgcc
     8401 gaaaagctgg cattttgttt cccatggtga aatgtgggtg cccatgccgg gcgatgcggc
     8461 ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg aatctggcga
     8521 aaatgccgca ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc gcgcgatgca
     8581 gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtta atgttgacag
     8641 cttcagcctc gaacaggcag tctaacgcgc caacttaagt gcagtcttcg gtacacagct
     8701 acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggca tgacttcgcc
     8761 ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat aagggatacg
     8821 aattccctga ttttctaatt gctccagcaa cacctgttgg ttatttccac gaaatgcctg
     8881 tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg cggtatcatc
     8941 agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca cctcatcgcc
     9001 aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga catcgccgtt
     9061 atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg gatgtttttg
     9121 ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa ccacatcaaa
     9181 caccacatca ccaatgcgaa tcactttcca gcgatcttct tcccaggctg acgcgccgga
     9241 aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac gttgttggag
     9301 atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa atgacagagg
     9361 tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc gtaattgcac
     9421 ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa ttcgcgcggt
     9481 aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag caaaacgaac
     9541 atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag gtgaaggagt
     9601 aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag gttccgtgat
     9661 cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg taagaccaat
     9721 gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca cggtgcggtc
     9781 ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct ataatgcgca
     9841 acaattttct tagcgcctgt cccattaggc tattttattt gtcagtttgg ccctgggttg
     9901 tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctgtc catgtccaga
     9961 ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga cgatatgaat
    10021 tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga actggaaaac
    10081 ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg cgacacacgg
    10141 cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt gccgctgggc
    10201 gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat caactggaca
    10261 gagatgttta atcctggcgc gaccttcgct gtccacttca gtggtttgaa tgacaccatc
    10321 cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc tttcacgcgg
    10381 aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt taacgtctgg
    10441 ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt acatctgcgt
    10501 ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc cgctattgtg
    10561 atgcgaaccg gctggcagcc aggaacaccg ctgctcgatc cgatgtgtgg ttccggtacg
    10621 ttgctgattg aagcggcgat gctagcgacc gatcgcgcac caggcttgca ccgtgggcgt
    10681 tggggcttta gcggctgggt gcagcatgat gaagctatct ggcaggaagt gaaagcggaa
    10741 gcgcaaactc gtgcccgtaa aggcctggct gagtatagct ctcattttta cggttcggac
    10801 agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg gattggtgaa
    10861 ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc gaaagggccg
    10921 tacggcacag tgttgagcaa cccgccatac ggggaacgtc tggacagcga accggcgctg
    10981 attgcgctgc atagcctgct gggtcggatc atgaaaaacc agtttggtgg ctggaatctc
    11041 tctttgttta gtgcctcgcc ggatctgcta agctgcttgc agctgcgtgc agacaaacag
    11101 tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt tgccgaaagc
    11161 actccagaca gcaaaccggc gatggtagcg gaagactacg ccaaccgcct gcgtaagaac
    11221 ctcaaaaaat tcgagaagtg ggctcgccag gaaggaattg aatgttaccg cctgtatgac
    11281 gccgatctac cggaatataa cgttgccgtt gaccgttatg ccgactgggt ggtggtgcag
    11341 gagtatgcgc cgccaaaaac tattgatgct cacaaagcgc gtcagcgtct gttcgatatt
    11401 atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct gaaaacccgt
    11461 gaacgccaga agggcaaaaa ccagtaccag aaactgggcg agaagggcga gtttcttgaa
    11521 gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga tactggtctg
    11581 ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg caaagatttc
    11641 ctcaacctgt tctcttatac cggtagcgcc accgtgcatg cgggattagg cggcgcgcgc
    11701 agcaccacca ccgtggatat gtcgcgtact tatctggagt gggcagaacg caacctgcgt
    11761 ctgaatggcc tgaccgggcg tgcgcatcgc ctgattcagg ccgattgcct ggcgtggctg
    11821 cgtgaggcaa atgaacagtt tgatctgatc tttatcgatc cgccaacctt ctctaactca
    11881 aaacgaatgg aagatgcgtt tgatgttcag cgcgatcata tggcgctgat gaaagatttg
    11941 aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg tggcttccgt
    12001 atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac gcaaaaaacg
    12061 ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat taccgcagcc
    12121 tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg ttcagcgacg
    12181 cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt tgtctggtgg
    12241 gtcgcaacgg cgcgggcaaa tcgacgttaa tgaaaatcct caaccgtgaa caagggctgg
    12301 atgacggtcg cattatttac gagcaagatt tgattgtagc tcgtctgcaa caggagccgc
    12361 cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa gaacaagcgg
    12421 aatatctgaa acgctatcac gatatttcgc acctggtgat gaacgacccg agcgagaaaa
    12481 atctcaacga actggcgaag gttcaggaac agttggatca ccacaacctg tggcagctgg
    12541 aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaatgtt gcgctgtcgt
    12601 cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg agtaatccgc
    12661 gcgtgctgtt gcttgatgaa ccgacaaacc acctggatat tgaaaccatc gactggctgg
    12721 aagggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac cgttcgttta
    12781 tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg acctatccag
    12841 ggaattacga ccagtacctg cttgaaaaag aagaagccct gcgcgtggaa gaactacaaa
    12901 atgccgagtt cgatcgcaaa ctggcgcagg aagaggtgtg gatccgccag gggatcaaag
    12961 cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcaatgcgt cgcgagcgtg
    13021 gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc agccgctccg
    13081 gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt tgacggtaag caactggtga
    13141 aagatttttc ggcccaggtt ctacgtggcg acaaaattgc cctgattggt ccgaatgggt
    13201 gcggcaaaac cacgctgctg aaactgatgc tcgatcagct tcaagcggac agcgggcgta
    13261 ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg gaactggatc
    13321 ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg gttaacggca
    13381 agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa cgggcgatga
    13441 cgccggtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg cgtttgttcc
    13501 tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat gtcgaaacgc
    13561 tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg gttagccacg
    13621 atcgtcagtt tgtcgataac accgttacag aatgttggat cttcgaaggc ggcggtaaaa
    13681 ttggtcgtta tgtcggcggt tatcatgatg cccgtggtca gcaagagcag tatgtggcgc
    13741 tcaaacagcc tgcggtgaaa aaaaccgaag aagccgccgc ggcaaaagca gaaactgtaa
    13801 aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag ctaccgcaat
    13861 tgctcgaaga tctggaggcg aagctggaag ccctacagac gcagatggcg gatgcttcct
    13921 tcttcagtca gccacatgag cagacacaaa aagtgctcgc cgatatggct gctgcagagc
    13981 aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa aatggtggct
    14041 gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg cctgatgaac
    14101 tgggtttatc aggcatgaaa accaacactt tgttagccat ctgtaaaata gcgcatcatt
    14161 aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc tcgcagtgtg
    14221 acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt ccccggtgtg
    14281 gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tccgaccgcc tatgcgttgg
    14341 ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg aacgttgcag
    14401 gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct gaggactacg
    14461 ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt tgtctgataa
    14521 ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag caactggcac
    14581 gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgct ggtgtgctgg
    14641 tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc tttctcccct
    14701 ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt cgctggttat
    14761 gggacgacat cgccccgatg ccagaactgc gccagccgct aaaaccaggc gtcacgggga
    14821 tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct gatgaacccg
    14881 tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagccag cagtggacac
    14941 tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg cccatcatgg
    15001 tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc attctgttat
    15061 ggagcgaagg ctcttatccc gtcgctgcgg tgatctttct ggccagtatt atggtgccaa
    15121 cgttaaagat gatcgccatc gcgtggctgt gttgggatgc caaagggcat ggcaagcgcg
    15181 acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc tggtcgatga
    15241 ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt ttaatgagta
    15301 tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca atgttttctg
    15361 ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag catgaggagt
    15421 cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa gaactggtct
    15481 cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct tttttatcat
    15541 tacagccatc agggactgga agtgaccctg atcaccgcga atgcggaagg aattgaaggt
    15601 ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag cgccacactg
    15661 gctgatgatt tgacgcacgt tgaaatcaaa gtgcggctga attctggtat ggaaaaattg
    15721 ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga agggattagc
    15781 ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc gaaaggcagc
    15841 aaaatggata aatacgattt gctggactcg ccaccgttgg ccccgcctga tgcgaaaggt
    15901 atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga tccggtgctg
    15961 ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca aaaacgcaat
    16021 atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag caatgttcgc
    16081 ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg tgtggagatg
    16141 ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga aggtctggat
    16201 ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca gaagagcatt
    16261 caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga ttcggtacgc
    16321 ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac cgtaagcaaa
    16381 gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg tattccggta
    16441 ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc ggatgttgtt
    16501 gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac cggaaacctg
    16561 gtcactggtg cactgtatgt tgatcttgat ttctatccaa atacgcctgc aataaccggt
    16621 attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggcct ggcgcaaatc
    16681 cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa tccgatgatt
    16741 gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct gcaaacgacg
    16801 ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc gacggatatg
    16861 cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg ctccgcagcc
    16921 tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga actgcaaccg
    16981 gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa ggacaaaaaa
    17041 gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt gcagcactgt
    17101 ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta cctgtggtgc
    17161 agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta gagcaggtca
    17221 ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat gtgaagtatg
    17281 tgattgccaa caacaacttg tgggccagcc cgttggatca acaattgcgc aacaccctgg
    17341 ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct ctgggaagcg
    17401 cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat ggcaaggtca
    17461 ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt ccgttccgtc
    17521 tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtactg gccagtgtct
    17581 ggagtcagga agccgcttct attgcgcaag agataaagcg tctaccttaa ttataaagat
    17641 ttgtaaatat aaccgtctcc ggtatgttgc ctgaggcggt ttttttgttt ctaacgtgcg
    17701 gaaaaagttg ttcctcttta cattttttgt acaaccgaca tacccgtgta gctcacaaat
    17761 atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta ttgcttaact
    17821 gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta tgagggaaac
    17881 gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt ggttatcagg
    17941 ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat caaaggtcac
    18001 aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc tgattctgtc
    18061 tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct gtctgattat
    18121 aatcagaagg cagaagtatc ctggaacaga ccgactttca ggtcgctggc ggtatagatc
    18181 aaacgaccat caaccagcac ttctccatcc gccaggccca taatcagacg acggttaaca
    18241 atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac ctgaccagtg
    18301 aatttcactt cgccaacgcc cagtgcgcgg cctttacctt cgccgcccag ccagccgagg
    18361 tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat aaccggatcg
    18421 ccaataaagt ggcatccgaa gaaccacaga tccggattga tatccagttc tgcttcaaca
    18481 taccctttgt cgaagttacc acccgtttcg gtcattttga ccacacggtc catcatcagc
    18541 atattcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgcg accagaggca
    18601 agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt aagccttatt
    18661 ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat cagttcggaa
    18721 taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc tgttgcgatg
    18781 cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg ccatcccaca
    18841 ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag atggtgaatt
    18901 tgccttcttc taccgctttc actagttcac tgtgaagact taaatggcga acgttagctg
    18961 tggggataat gacaccttgt ttcccggtta actcacgttg ctggcaaata gcaaagaagc
    19021 cttcgatttt ctcatttaaa ccaccgaccg gctgggcgcg accgaactga tcgactgaac
    19081 ctgtgatagc gatactctga ttcaccggca catcggcgag ggcgcttatc agggcgcaga
    19141 gttcagccat cgaggcacta tctccatcaa cttcactgta tgactgctca aatgtcagcg
    19201 atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac gcttgcatga
    19261 tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg atgtcggtga
    19321 attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca aaagcgcgtg
    19381 gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt tcgccttcgg
    19441 tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg aggaaacctt
    19501 cgcgccattc acgctgctgc agcattaagt ttagctgctc gccggagaag gtgtcgccat
    19561 cacacaggga ggcgacctct ttacactggc ggaggatcca ctgcgggcta agcggaagtg
    19621 tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccag gcatccgctc
    19681 ccggtgcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac cactgggtta
    19741 ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata gcctgctctg
    19801 aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct accagaatga
    19861 ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac tcatcaaacg
    19921 caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac agcagaggtt
    19981 gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga tgcaccagac
    20041 caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt tgctccgctt
    20101 ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct gcactcactg
    20161 cgtgacgtaa gcggatggag tggccggaaa cctcatagtg accgcccacc agttggcctg
    20221 catcgctttg tagcgtacgc gcggcattgg caataagatt cagatactca gactcttccg
    20281 gggccttcgc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc tccagcgcaa
    20341 attgcagccg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc aaatgtggct
    20401 gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat gcaagtttcg
    20461 taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata ccgtaaccgg
    20521 cactacagca cacgtagaaa gcaccgacaa tactccaggc atgggcgtta aagctcacag
    20581 gatggagatt cttttcttca ctggcctaaa aagctgatat tctgtaaaga gttacacggt
    20641 aacattgaga tcgctatgaa atatcaacaa cttgaaaatc ttgaaagcgg ttggaaatgg
    20701 aagtatctgg tgaagaagca tcgcgaaggg gagttaatca cccgttacat agaagccagt
    20761 gccgcccagg aagccgtcga tgtgttgctc tcgctggaaa atgaacccgt gctggtaaat
    20821 ggctggattg acaagcatat gaatccggaa ctggtcaatc gcatgaagca aaccattcgg
    20881 gcaagacgaa agcgccattt taatgcggaa caccagcata cgcgcaaaaa atcgatcgat
    20941 ctggaattta ttgtctggca acgtctggct ggtcttgcgc agcgtcgcgg taaaacgctt
    21001 tctgaaacga ttgttcagct gattgaagat gcggaaaaca aagagaaata cgcgaataaa
    21061 atgtcttctc tgaagcagga tctgcaggca ttgctgggta aggaataact gacgaaagtc
    21121 agttcaattt actaaaggca aaaaaaaccc cgcagcagcg gggtttttct accagacgag
    21181 aacttaagcc tgcggctgag ttacaacgtc tttgatacct ttaacttcga tctctacgcg
    21241 acgatccgga gccaggcagt cgatcagtgc agcacgctgt ttcacgttgt cacaggtgtt
    21301 gccagtaacc gggttggatt cgcccatacc acgtgcggag atcttgtctg ccgggatacc
    21361 tttggagatc aggtaatcaa caacagactg agcacggcgc tcggacagac cctggttgta
    21421 agcgtcagaa ccgatgcggt cggtgtaacc cagaacaact acggaaccgt ctttcggatc
    21481 caggttgctc agctggctgt acagctgatc cagagcagcc tgaccttccg gtttcagggt
    21541 tgctttgttg aagttgaaca gaacgtcaga cttcagagtg aagtgcttgg tctgtacttc
    21601 cggtgccgga gctggagccg gagcaactac tggagctgct tcgccctgac cgaaacggta
    21661 ggaaaccccc aggctcagca tgccgttgtc cggacgagtg ccgatggtgt gtgcgtcacc
    21721 gatgttgttg gtccactggt attccagacg ggtagcgatt tcaggagtga tcgcgtactc
    21781 aacaccgcca gcgaagaccg gagaaacgcc ggtgtcgtgg tttttaccat aaacgttgga
    21841 tttagtgtct gcacgccata ccataccacc cagacgagtg tagatgtcca ggtcgtcagt
    21901 gattgggtaa cccagtttag cggtcagttg aacgccctga gctttgtatg caccgttttc
    21961 aacgctgcct ttgtacggca tacgacctaa ccagtcgtaa cccatttcaa agccaacata
    22021 cgggttaacc tggtaaccac caaaagcacc agcgcccagt tggttttcat gggtcgggcc
    22081 attgttgttg atgaaaccag tgtcatggta ctgggaccag cccagtttag caccagtgta
    22141 ccaggtgtta tctttcggag cggcctgcgc tacggtagcg aaaccagcca gtgccactgc
    22201 aatcgcgata gctgtctttt tcattttttg cgcctcgtta tcatccaaaa tacgccatga
    22261 atatctccaa cgagataaca cggttaaatc cttcaccggg ggatctgctc aattaactct
    22321 accgatatct tcggcttatg ccgagcaccc ctggcgatgt aaagtctaca acgtagttga
    22381 aaacttacaa gtgtgaactc cgtcaggcat atgaaaaaaa agtcttgtat aaggtatgtt
    22441 taatcttttt tgtcagcgac aatttacaga agaaaatcgc ggaaaccgct tcagacaagc
    22501 ctccgcaagg aaaattagtc acgactgaaa gcattggctg ggcgacaaaa aaagttccag
    22561 gattaatcct aaatttactt aatgatacaa attagagtga atttttagcc cggaaagttg
    22621 tctcgtggcg tgagaggatg cgcttaccgg acgcataata aaccccatag cgttaccttc
    22681 atttgccgca tcaacaagtt cagcatgctc ttcttcagtc aaatcatctg ccaaccaacc
    22741 gatcaccaca ctgtaattgc ccgtgcgtaa agcgcgaacc attgactcca cagtgtggca
    22801 aggggagagc tggctaatct gcattacttt cgttaagggt agcccagatg cctgaaccca
    22861 ttcccgactc agtttttgtt gcggtgttaa ccagagttgc cagcgcgatt gctgaccgag
    22921 ttgctgtaac aatggcaaca gtagaagttg cgtcatcatg ggctgatctt cgcgatagac
    22981 aacttcactg ataagcccgg ctgtagtgtt ttccgtagag acacgcgcaa ttttacttgc
    23041 tgcggatgag aacgacgaag aacgatgtgc atagcctgaa gtgtacataa tcaatccagc
    23101 ccctatgagt tactgtatgg atgtacagta catccagtga caacaaagat caaccctatt
    23161 ttcggaaaga gcctcgcaaa ttttgtcgtt ggtgacggga aaacataaat taatcttgcc
    23221 ccttaagaat aagttgccta ttttcgtagt taacggatcc gttaatgtga atcattcttt
    23281 tatgttatga ttttaaaagg aattttatga aaagcctctc ctataagcgg atctataaat
    23341 cacaagaata cctggcaacg ttgggcacaa ttgaataccg atcattgttt ggcagttaca
    23401 gcctgaccgt tgacgacacg gtgtttgcga tggtttctga tggtgagttg tatcttcggg
    23461 cttgtgagca aagtgcacag tactgtgtaa aacatccgcc tgtctggctg acatataaaa
    23521 agtgtggccg atccgttacc ctcaattact atcgggttga tgaaagtcta tggcgaaatc
    23581 aactgaagct ggtgcgtctg tcgaaatatt ctctcgatgc agcgctgaaa gagaaaagca
    23641 cgcgcaatac ccgggaaaga ctgaaagatt tgcccaatat gtcttttcat ctggaagcga
    23701 ttctcgggga ggtggggatt aaggatgtac gggcgttacg tatacttggg gcaaaaatgt
    23761 gttggttgcg actgcggcag caaaacagtc tggtgacaga aaagattctg tttatgcttg
    23821 aaggtgccat tatcggcatt catgaagctg cgctcccggt ggcacgccgc caggagcttg
    23881 cagaatgggc tgactctctt acgccgaaac aggagtttcc tgcggaactt gagtaatctg
    23941 gcgttgcaga cgaccaatct caggcagtaa tgcaatcaat aatccaactt gttgtacgac
    24001 cagaggttct ttgctgtctg cccgtggttc aagttgctgc atccgctgtt tcaggctagc
    24061 taatgcctca ttgacgcgtt cttcatcagc aggttgatga tgtaacgcgt catcaacata
    24121 gcaaactgcg tcatcaagaa acgccagaat ttcaggatta gttaactgct cccggtgagc
    24181 accgagggct gagatatagc tggtaaacgt atggttaagg cacagcaacc gaaacgcggc
    24241 ttcgcgaatt tgcggggtaa cgttcggctc gctggacata tttgatacca ccgacgccag
    24301 ctcagcatca cggttgtgtg catcgcggcg ggcaatacga tacgccagac ggttatcacg
    24361 cccctgatgg tattgctcca gtatggcatc gagataccga cagttggcct ctgtggcgcg
    24421 ttcgagcatg cgcggcagat tgcgaaactg ccagtcaggc cagatgtagc tcactgccgc
    24481 ccacgcaatg gcacaaccaa tcagcgtatc gattacgcga ggtaacgcta cttcaaaacc
    24541 ttcacccagt aagttaaaac acagtagcac caaaagtgtg atgaacatcg ttgcatgagc
    24601 gtattgcacg ttacggaagg caaaaaagag cacgccggta ataaccagca gcaccagctg
    24661 cccttccagt gatggcacaa accacagcac aggaatgcca atggcgatac ctaccagcgt
    24721 accaataatc cttaacttca ggcggtggcg cgtggcgtta tagtttggct ggcagacaaa
    24781 caaacttgtc agcaagatcc aatacccgtg atgcattccg gttatctgaa tgatggcgta
    24841 gccgaagcac aataccagcg acattcttac cgcatgacgg aagagggcgg attccggcgt
    24901 gaagtgacgg ctaagacgca gccagatatc actcaacccg tgcgggctgt catcagcgag
    24961 ctcattttcg tcattattat ggggtagtgc ctgggcctgt tctgattcaa ttgttgccag
    25021 ttgggcatca atggcgcgta aattgttcag caaaaatccc agtgttttga gtaaatcggc
    25081 gggtgcgccg ttatcgcgca tccgctccag cgcagcatca atatgcgtaa aagcgcgctc
    25141 aaaatgcgga tcatgttgat aaggctgacg caacaaaata cagcgtgaca gttgctggca
    25201 cgcctggccc tgcatcgaca tcagccgctg aaaacggaac agcacgtcgc tgtggcgaaa
    25261 atgttcacgc aatgtttgat actgaatatg agaagagctg gcacgctcgt gaatatcctg
    25321 tgcgacaaag taataatgca gcgtgcgacg cgttccccgt tgaccacgat cgccacgtaa
    25381 gcgggtcagc agcgagagtt tcgtctgatt caatgtcgcc atcagcagac cgttggcgag
    25441 agccaaatcg tacagcggtg cctggctttc atcttcaata tcaggatcaa acatgcgcga
    25501 cttgagctca agataacgcg ccagttgttc atagcaacgc gccaggttgt cctgcagcgg
    25561 gcggaccggg aacagcagat gaccaataag tgtcaggacg ttgtaccaga cggcaccggc
    25621 cagcagatac atcggctgct gataccagtg ctcatacagt gatgttccca acatagtgta
    25681 aatggcgatc agcaatgcac cgaaggcaat tgttgcatag cgttgaccca gaccgccgag
    25741 caaaatgaag ccgctggtag agagcgttaa gccaatcgca aatagccagg gccagggaaa
    25801 cagcaattct actgaggccg aggcgataaa aaagcagaac agcgtaatga tgaggttacg
    25861 caaacgtccc gccagtcggt catcgagatc ggtcagcgcc gctgccacca tccccagcgt
    25921 tagcggaatc gtcagtttta catcacccag ccaccacgga aacgctgtgg ttccacaaag
    25981 cgcaataaaa atacgcgcgt aatacagcca ggcgctgttc caggtatagc gtttgagcaa
    26041 aggacttagc ataaaggcca tattagtgga ttattcaaaa cgacgacgtg cattggcttc
    26101 acgcgcagct tgcgctgttt ctaccgatac cacgcgacga ccaaccggcc atagtgcaat
    26161 agcggcaatt ttaaagttcg caatgccgac aggaatgcca atgattgaaa tacattgtgc
    26221 gatgcccgtt gcaatgtgca tcaggcataa ccaccagcca aagaaaatca gccagaaaat
    26281 attcaatacc gtaccgccag tattcagcag cacatttttg ccagccgggt tcagttcatc
    26341 gacatgaata gcttcattgc cataaggcac cagagatagt ttagtgatct cccagcagga
    26401 tcgtgtcagc ggtaaggtaa aaatcagcac aatactgacc agagtcgcca acagccagcc
    26461 cagagtggtg gcaaatccgc caagcacaaa gttcagaatg ttcaaaacgg tacgcataaa
    26521 acctcgcttt actgtggttt tcagtaatgg gcggcaattg taacgttttt ttgggctgga
    26581 gcacgttttc tctgacggtt acactgataa gaaataattt cgtgtggatc tacagagtca
    26641 tggaactgaa agcgacaacg cttggaaaac gtctggcaca gcacccttac gatcgggcgg
    26701 tgatcctcaa tgccgggatt aaagtctccg gcgatcgcca cgaatacctt attcctttca
    26761 atcaattact ggcgattcac tgtaagcgcg gtctggtatg gggcgagctg gaatttgtac
    26821 tgccggacga aaaagtggtg cgtctgcacg gcaccgaatg gggcgagacg cagcgttttt
    26881 accatcatct tgatgctcac tggcggcggt ggagtggcga gatgagcgaa attgcgtctg
    26941 gtgttttacg ccagcaactg gatttgattg ccacgcgcac tggagaaaat aaatggctga
    27001 cgcgtgagca aacctctggc gttcagcaac aaatccgcca ggctttgtcg gcgttgccgt
    27061 tgccggttaa ccgactggaa gaattcgata actgccgtga ggcgtggcgt aaatgtcagg
    27121 cctggttgaa agatattgaa agcgctcggt tgcagcataa ccaggcgtat accgaagcca
    27181 tgcttaccga gtatgcggat tttttccgcc aggtcgagtc ttcaccgctg aatccggcgc
    27241 aggcccgggc agtcgttaat ggcgagcatt ctctgttagt gctggcaggt gcaggaagcg
    27301 gaaaaacgtc ggtgctggtg gcccgtgcag gctggttgct ggcgcgtggt gaagcgtccc
    27361 ctgagcaaat tttattgctg gcgtttggtc gcaaagccgc tgaagagatg gacgagcgga
    27421 ttcgcgaacg gctacatacc gaagacatta ccgcacgcac gtttcatgcg ctggcgctgc
    27481 atattattca gcagggcagc aaaaaagttc cgatagtcag caaactggaa aatgataccg
    27541 ctgcccgtca tgaactcttt attgctgagt ggcgcaagca atgcagcgaa aagaaagcgc
    27601 aggcgaaggg ctggcggcaa tggctgacgg aagaaatgca gtggtcagtg ccagaaggta
    27661 acttctggga tgatgaaaaa ttacagcgtc gccttgcctc tcgcctcgat cgctgggtaa
    27721 gtctgatgcg gatgcacggt ggtgcacagg cagaaatgat tgccagtgca cccgaagaga
    27781 ttcgcgatct gttcagtaaa cgtatcaagt tgatggcccc gttattaaaa gcctggaaag
    27841 gtgcgctgaa ggcagaaaac gctgtcgatt tttcgggcct tattcatcag gcgattgtga
    27901 ttctggagaa aggtcgcttt atcagcccgt ggaagcatat tctggttgat gaatttcagg
    27961 atatctcgtc gcagcgggca gcgttgttag cggcattacg caagcaaaac agtcagacga
    28021 cgttgttcgc tgttggtgat gactggcagg cgatttaccg attcagcggt gcgcaaatgt
    28081 cgctcaccac cgctttccat gaaaactttg gtgaaggcga acgttgtgat ttagacacga
    28141 cttaccgttt taacagtcgt atcggtaagg tggcaaaccg gtttattcag cagaacccag
    28201 gccagctgaa aaagccgcta aacagcttaa ccaatggaga caaaaaagcc gtcacgttat
    28261 tggatgagag tcaacttgac gctttgctgg ataagctctc tggttatgcc aaaccggaag
    28321 agcgcattct gatcctggcg cgttaccatc acatgaggcc tgccagcctg gaaaaagcgg
    28381 caacacgctg gccgaagttg caaatcgact ttatgaccat tcatgccagc aaagggcaac
    28441 aggcggatta cgtcatcatc gttggcttgc aggagggaag tgatggtttt ccggctgcgg
    28501 cgcgggagtc gattatggaa gaggcgctac tgccaccggt tgaggatttc ccggacgctg
    28561 aagaacggcg gttaatgtac gtggcgctga cccgggcacg ccatcgggta tgggcactgt
    28621 ttaacaaaga gaatccctct ccctttgtgg aaatactgaa aaatctggat gtgccggtgg
    28681 cgagaaaacc gtaagaaaca ggtggcgttt gccacctgtg caatattact tcagacggtc
    28741 cgcgagataa cgctgataat cggggattag aatatcgacc gcgtcgttga aatgcggcga
    28801 ctggattatg aagtctgccg ttgccacgtt ggtggcgacc ggaatgttcc ataccgtcgc
    28861 cagacgcagc aaggctttca cgtcaggatc gtgcggcacg gcatttagtg gatcccagaa
    28921 gaaaatcaat acatcaattt tcccttctga gatcaatgcg ccaacctgct ggtcaccccc
    28981 cattgggcca ctcaacatcg cgttgacgtt catgccggtc gcgcgggaaa ttaagttacc
    29041 ggtagtgcct gttgcataca gtacgtgttg ttccagtaac ggttgatgcc gttccaccca
    29101 gctcatcagc atttgtttgc agtgatcgtg tgccaccagc gcaatatgtt tccgcgcagg
    29161 taaagtgcga gtcgtcagtt ccataatgta catccgtagt taactttcct acagattact
    29221 gtaagcactt atcgctgcaa gataaagacc gaaaaagcct gcgcacaggc acaaaaatct
    29281 caggaagatg gttgtttttc cgcccactgc aggaaagtat ttcgcgtttg tgggtcagcc
    29341 agtttaaacc aatacttcag ccgttgttct gtgagcacct gagactgcgg tggaatagca
    29401 tccagctcgg aaacacccga aagcagcgta ctgtcatctg ccatcatcgt ggcaaattgc
    29461 ggtagtgaag cgcgtttcgc ggatttgtta tagcgttcaa cctcgacctc ataatcaatc
    29521 gtttttgccg ttgaggtaat ggcgagaata tccagcttta ccggaatcgg cgtcgcatcg
    29581 ccatccaaca attcaaggcg cggcgcggca tcaaaatggt tagcttcccg ctcattctcc
    29641 aggcgaggca ggcgaaaatt gacctggttg atgagctggg tattaaaact gaccaccagc
    29701 ggtggggaga tatacagccg ttcttcacta ttggaaagat gaatcgtctt ctcaacgcga
    29761 aacactaact gatgcggtcc attatccagt tcaatgctgt ccgcaccgcg cagcagagag
    29821 ctggaaacct tcttgccgtc gagtaccaga agatcgacat cggttgaaag ccgcagcgtg
    29881 gtggcaaaaa cggataccgg caaacataat gcaattaagg tggtcacgat gccggttttc
    29941 ataggtagct cctgtcaaaa gaccgcccga ataataatgt gtcaaaattt ttctttaatc
//
LOCUS       DAETQN010000043        29548 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.043, whole genome shotgun sequence.
ACCESSION   DAETQN010000043 DAETQN010000000
VERSION     DAETQN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.043"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="K0W68_001921"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="K0W68_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HBI3073485.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="K0W68_001922"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="K0W68_001922"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBI3073486.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="K0W68_001923"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="K0W68_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HBI3073487.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="K0W68_001924"
     CDS             complement(3644..4030)
                     /locus_tag="K0W68_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBI3073488.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="K0W68_001925"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="K0W68_001925"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HBI3073489.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="K0W68_001926"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="K0W68_001926"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBI3073490.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="K0W68_001927"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="K0W68_001927"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBI3073491.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="K0W68_001928"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="K0W68_001928"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBI3073492.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="K0W68_001929"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="K0W68_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBI3073493.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="K0W68_001930"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="K0W68_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBI3073494.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="K0W68_001931"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="K0W68_001931"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBI3073495.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="K0W68_001932"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="K0W68_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBI3073496.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="K0W68_001933"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="K0W68_001933"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBI3073497.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="K0W68_001934"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="K0W68_001934"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBI3073498.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="K0W68_001935"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="K0W68_001935"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBI3073499.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="K0W68_001936"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="K0W68_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBI3073500.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="K0W68_001937"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="K0W68_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBI3073501.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="K0W68_001938"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="K0W68_001938"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBI3073502.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="K0W68_001939"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="K0W68_001939"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBI3073503.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="K0W68_001940"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="K0W68_001940"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBI3073504.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="K0W68_001941"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="K0W68_001941"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBI3073505.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="K0W68_001942"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="K0W68_001942"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBI3073506.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="K0W68_001943"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="K0W68_001943"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBI3073507.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="K0W68_001944"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="K0W68_001944"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBI3073508.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="K0W68_001945"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="K0W68_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBI3073509.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7120 c         8160 g         7109 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgataaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcctgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttctcgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAETQN010000044        28814 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.044, whole genome shotgun sequence.
ACCESSION   DAETQN010000044 DAETQN010000000
VERSION     DAETQN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.044"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(406..612)
                     /locus_tag="K0W68_001946"
     CDS             complement(406..612)
                     /locus_tag="K0W68_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBI3073510.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            925..1014
                     /gene="kdpF"
                     /locus_tag="K0W68_001947"
     CDS             925..1014
                     /gene="kdpF"
                     /locus_tag="K0W68_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBI3073511.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1014..2687
                     /gene="kdpA"
                     /locus_tag="K0W68_001948"
     CDS             1014..2687
                     /gene="kdpA"
                     /locus_tag="K0W68_001948"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBI3073512.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2710..4758
                     /gene="kdpB"
                     /locus_tag="K0W68_001949"
     CDS             2710..4758
                     /gene="kdpB"
                     /locus_tag="K0W68_001949"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBI3073513.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4767..5339
                     /gene="kdpC"
                     /locus_tag="K0W68_001950"
     CDS             4767..5339
                     /gene="kdpC"
                     /locus_tag="K0W68_001950"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBI3073514.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5332..8016
                     /gene="kdpD"
                     /locus_tag="K0W68_001951"
     CDS             5332..8016
                     /gene="kdpD"
                     /locus_tag="K0W68_001951"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HBI3073515.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            8013..8690
                     /gene="kdpE"
                     /locus_tag="K0W68_001952"
     CDS             8013..8690
                     /gene="kdpE"
                     /locus_tag="K0W68_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBI3073516.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8965..9069
                     /locus_tag="K0W68_001953"
     CDS             8965..9069
                     /locus_tag="K0W68_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBI3073517.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9380..11578
                     /gene="speF"
                     /locus_tag="K0W68_001954"
     CDS             9380..11578
                     /gene="speF"
                     /locus_tag="K0W68_001954"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HBI3073518.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11575..12894
                     /gene="potE"
                     /locus_tag="K0W68_001955"
     CDS             11575..12894
                     /gene="potE"
                     /locus_tag="K0W68_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBI3073519.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12950..14590)
                     /gene="pgm"
                     /locus_tag="K0W68_001956"
     CDS             complement(12950..14590)
                     /gene="pgm"
                     /locus_tag="K0W68_001956"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBI3073520.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14616..15161)
                     /gene="seqA"
                     /locus_tag="K0W68_001957"
     CDS             complement(14616..15161)
                     /gene="seqA"
                     /locus_tag="K0W68_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBI3073521.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15346..16110
                     /gene="ybfF"
                     /locus_tag="K0W68_001958"
     CDS             15346..16110
                     /gene="ybfF"
                     /locus_tag="K0W68_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBI3073522.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16250..16543
                     /gene="ybfE"
                     /locus_tag="K0W68_001959"
     CDS             16250..16543
                     /gene="ybfE"
                     /locus_tag="K0W68_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBI3073523.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16683..17213
                     /gene="fldA"
                     /locus_tag="K0W68_001960"
     CDS             16683..17213
                     /gene="fldA"
                     /locus_tag="K0W68_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBI3073524.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17423..17509
                     /gene="uof"
                     /locus_tag="K0W68_001961"
     CDS             17423..17509
                     /gene="uof"
                     /locus_tag="K0W68_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBI3073525.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17502..17948
                     /gene="fur"
                     /locus_tag="K0W68_001962"
     CDS             17502..17948
                     /gene="fur"
                     /locus_tag="K0W68_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBI3073526.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18033..18359)
                     /locus_tag="K0W68_001963"
     CDS             complement(18033..18359)
                     /locus_tag="K0W68_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073527.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18409..19815)
                     /gene="chiP"
                     /locus_tag="K0W68_001964"
                     /pseudo
     CDS             complement(18409..19815)
                     /gene="chiP"
                     /locus_tag="K0W68_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(20393..22057)
                     /gene="glnS"
                     /locus_tag="K0W68_001965"
     CDS             complement(20393..22057)
                     /gene="glnS"
                     /locus_tag="K0W68_001965"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBI3073528.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22317..24263)
                     /gene="nagE"
                     /locus_tag="K0W68_001966"
     CDS             complement(22317..24263)
                     /gene="nagE"
                     /locus_tag="K0W68_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBI3073529.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            24596..25396
                     /gene="nagB"
                     /locus_tag="K0W68_001967"
     CDS             24596..25396
                     /gene="nagB"
                     /locus_tag="K0W68_001967"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBI3073530.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25456..26604
                     /gene="nagA"
                     /locus_tag="K0W68_001968"
     CDS             25456..26604
                     /gene="nagA"
                     /locus_tag="K0W68_001968"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBI3073531.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            26613..27833
                     /gene="nagC"
                     /locus_tag="K0W68_001969"
                     /gene_synonym="nagR"
     CDS             26613..27833
                     /gene="nagC"
                     /locus_tag="K0W68_001969"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBI3073532.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27881..28633
                     /gene="nagD"
                     /locus_tag="K0W68_001970"
     CDS             27881..28633
                     /gene="nagD"
                     /locus_tag="K0W68_001970"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HBI3073533.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         6751 a         7352 c         7561 g         7150 t
ORIGIN      
        1 gcaccgagca ggggattgac cggatggccg gacgtcactc cgccggggca caccgaacag
       61 gccacaccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatttctca tccttgtttt
      181 atcgggttat ttgtccgctg acaggcaaga ccctacagat ttataagaaa gccttaagtg
      241 agatatccgt cagatagaaa caattaggaa aatatttata tatatttcat gaatatatgt
      301 ttcgattggt ataacggtgt ctaaatattc ctctgaacat cttcctgata caaaaaaccg
      361 ccagcgtttc cgctggcggt tttcaatgta ttcagaactc agcaatcagt aaaaatcacc
      421 ggttgccttt tcggcctgat ccatccacac cggtttatcg ctggttttcg accagacgcg
      481 gtgcaggtag ctgtaaaagc gggcgcggtc tttccagaaa agcatgaaag gcagcgccag
      541 aacgcccgct gcaaccgcat atgtacggcg taaaaagata agccatgcag gatattctct
      601 gtagagttcc atacttttct cccccttttg tatctacccg gtgaatagca ccggaaaaat
      661 gaatttgttt atctgatgaa aatagtaccg ccttttgtgt aattttacta ctcatccgac
      721 cacttatttt tgcttattga tggtttattt acattcactc tgtaattaag ttacacaaaa
      781 gttaaattaa tactaaacat tagttaaatc atggcttttg ccatttttat acttttttta
      841 caccccgccc gcagattttt gcgaaatctt tgcagccaga attctactct tccggtatca
      901 cttttaggcc actggaggtg cactgtgagt gcaggcgtga tagccggcgt gttgctggtg
      961 tttttattac tgggttattt ggtttatgcc ctgatcaatg cggaggcgtt ctgatggctg
     1021 cgcaagggtt cttactgatt gccacgtttt tactggtatt aatggtgctg gcgcgtcctt
     1081 taggcagcgg gctggcgcgg ctgattaatg acattcctct tccaggtaca gcgggcgttg
     1141 aacgtatact ctttcgttta cctggcgtct ctgaccatga gatgaactgg aagcaatatc
     1201 tttgtgccat tctcggcctg aacatgctgg ggctggcggt gctgtttttt atgttgctcg
     1261 gtcagcacta tctgccgctt aatccacagc agttgccggg gctgtcgtgg gatctggcgc
     1321 tgaataccgc cgtcagcttt gtcacgaata ccaactggca atcttatagc ggtgaaacca
     1381 cgttgagcta tttcagccag atggcaggct taacggtaca aaactttctt tctgccgcca
     1441 gcgggattgc ggtgattttt gccttcatcc gtgcgtttac tcgccagagc atgagcacac
     1501 tcgggaatgc ctgggtcgat ctgctacgca tcacgttatg ggtgctagtc cctgtggcgt
     1561 tgttgatcgc actgtttttt attcaacaag gtgcgctgca aaactttctg ccctatcagg
     1621 cagtgaatac cgttgaagga gcgcaacagc tgttacccat gggaccagta gcttctcagg
     1681 aagcgatcaa gatgctcggt actaacggcg gtggcttctt taatgccaac tcgtcgcatc
     1741 cgtttgaaaa cccaaccgca ctgaccaact tcgtgcagat gctggcgatc ttcttgatcc
     1801 caacagcgct gtgctttgcc tttggtgaag tgacgggcga tcgccgccag gggcgcatgt
     1861 tgctgtgggc gatgtcagtg atttttgtca tctgcgtagg cgtggttatg tgggcagaag
     1921 ttcagggtaa tcctcatctg ctggcgctcg gcgcagacag cagcatcaat atggaaggta
     1981 aagagagccg tttcggcgtg ctggtcagta gcctgtttgc ggtcgtgact acggcggcgt
     2041 cctgtggcgc ggtgattgcg atgcatgatt cgtttaccgc tctcggcggc atggtgccga
     2101 tgtggctaat gcaaattggt gaagtggtgt tcggcggtgt cggttctggt ctttacggca
     2161 tgatgctgtt tgtcctgctg gcggtgttta ttgccgggct gatgattggt cgtacaccgg
     2221 aatatctggg taaaaaaatc gacgtacgcg agatgaaact gactgcactg gcgattctgg
     2281 ttactccaac actggtactg atgggcgcgg cgttggcgat gatgaccgac gccggacgta
     2341 gcgccatgct taaccctggc ccgcatggtt ttagcgaagt gctgtacgcc gtgtcgtccg
     2401 ccgctaacaa caacggcagc gcctttgccg gattaagcgc caactctccg ttctggaact
     2461 gtttactggc gttctgcatg tttgtcggtc gcttcggggt gattatcccg gtgatggcaa
     2521 ttgccggttc gctggtgagt aaaaagagcc aacccgccag ctccggcacg ctgccaacgc
     2581 acggcccgct gtttgttggc ctgttaatcg gcaccgtgtt gctggttggc gcactgacct
     2641 ttatccctgc cctggcgctt ggtccggtgg cggaatatct ctcctgatga tattgagcga
     2701 gcactgaata tgagtcgtaa acaactggcg ctattcgaac caacacttgt cgttcaggcg
     2761 ctgaaagaag cggtgaaaaa attaaacccg caggcgcaat ggcgcaatcc ggtgatgttt
     2821 atcgtctgga tcggcagtct gctgaccacc tgtattagca tcgcgatggc aagcggtgcg
     2881 atgcctggca atgcgctgtt tagcgcggca attagcggct ggctgtgggt caccgtactg
     2941 ttcgctaatt tcgccgaggc gctggcagaa ggccgcagta aagcgcaggc caacagtctt
     3001 aaaggggtga aaaaaactgc ctttgcccgc aagctgcgtg agccgaaata tggcgctgcg
     3061 gcggacaaag ttcctgccga tcaactgcgt aaaggtgaca tcgtgctggt agaagctggc
     3121 gatattatcc cctgcgatgg tgaagtcatt gaaggcggcg catcggtcga tgaaagcgcc
     3181 atcaccgggg aatcggcacc ggtgatccgt gaatccggcg gcgattttgc ctccgtcacc
     3241 ggcggcacgc gtattctttc tgactggctg gtgattgagt gtagcgttaa ccccggtgag
     3301 acatttctgg atcggatgat cgcgatggtg gaaggcgcac agcgacgcaa aacaccgaac
     3361 gagattgccc tgactatcct gctgattgct ctgaccatcg tctttttact ggcaaccgcc
     3421 acgctgtggc cgttttccgc gtggggcggt aatgccgtga gtgtaacggt actggtggcg
     3481 ctgctggtct gtctgatccc aaccactatt ggcggcctgt tgtcagcgat cggcgtcgcc
     3541 gggatgagtc ggatgctggg cgcgaatgtg attgccacca gcggacgtgc agttgaagcg
     3601 gcaggtgacg ttgacgttct gctactggat aaaaccggca ccatcacact cggtaaccgt
     3661 caggcgtcgg agtttatccc cgcgcagggc gtggatgaaa aaacgctggc tgacgccgca
     3721 caactggctt cgctggctga tgaaacgccg gaaggtcgca gtattgtgat cctcgccaag
     3781 caacgtttta acctgcgcga gcgcgatgtg cagtcgctcc atgccacctt tgtaccgttt
     3841 actgcgcaaa gccggatgag cgggatcaac atcgacaacc gcatgatccg taaaggttct
     3901 gtcgatgcca ttcgccgcca tattgaggct aacggcggtc acttccctgc cgatgttgat
     3961 caaaaagtcg atcaggttgc gcgtcaggga gccacgccgc tggtggtggt ggaaggttct
     4021 cgtgtgctgg gcgttattgc gctgaaagat atcgtcaaag gcggtattaa agagcgcttc
     4081 gcccagctgc gcaaaatggg cattaaaacg gtgatgatta ccggcgataa ccgtctgact
     4141 gccgccgcga tagctgcgga agcgggtgtc gatgattttc tcgccgaagc aacaccggag
     4201 gccaagctgg cattgatccg tcagtatcag gcggaaggtc gtttggtagc gatgaccggc
     4261 gacggcacca acgatgctcc ggcgctggcg caggcagatg tcgcggtggc gatgaactcc
     4321 ggcacccagg cggcgaaaga ggcgggcaat atggtggatc tcgactctaa cccgaccaag
     4381 ttgatcgagg tggtgcacat tggcaaacag atgctgatga cccgtggctc gctgaccacc
     4441 ttcagcattg ccaacgatgt ggcgaaatac ttcgccatta ttccggcggc gttcgcggca
     4501 acatatccgc agttaaatgc gctgaacatc atgcgcctgc attcgcccga ctccgccatt
     4561 ctcagtgcgg tcattttcaa cgccttgatt atcgtctttt tgattcctct ggcgttaaaa
     4621 ggcgtgagtt ataaaccgct taccgcttct gccatgttgc gccgtaactt atggatttac
     4681 ggtctgggtg ggctgctggt gccgtttatt ggtatcaaag tcattgattt actgctgacc
     4741 gtttgcggtc tggtgtgagg tttaccatga gtggattacg tccggcatta tcgacatttc
     4801 tctttctgtt attgattact ggcggcgttt acccgctgct gaccaccgca ctggggcaat
     4861 ggtggtttcc ctggcaggcc aacggttcgt tgatccgtga aggtgatacg gtgcgtggct
     4921 cggcattaat cgggcagaat tttaccggca acggctattt tcatggtcgc ccgtcagcaa
     4981 cggctgaaat gccctataat ccacaggctt ctggcgggag caatctggcg gtcagtaacc
     5041 ctgagctgga taaactaata gccgcacgcg ttgctgcatt acgggccgct aacccggatg
     5101 ccagcgcgag cattccggtt gaactggtga cggcatcggc aagcgggctg gacaataata
     5161 tcaccccgca agcggcggcc tggcagatcc cacgcatcgc gaaagcgcgt aatctcagcg
     5221 ttgaacagct cacgcagctg atcgcaaaat acagccaaca accgctggtg aaatatatcg
     5281 gccagccggt cgtcaacatt gtcgaactca atctggcgct ggataagctt gatgaataac
     5341 gaacccttac gtcccgaccc cgatcgtctg ctggaacaaa ctgccgcgcc gcatcggggg
     5401 aagctgaaag ttttcttcgg tgcctgtgca ggcgtcggga agacctgggc gatgctggca
     5461 gaagcccagc gactgcgggc gcaagggctg gatattgtgg ttggcgtggt agaaacccac
     5521 gggcgaaaag ataccgccgc aatgctggaa gggctggctg ttctgccgcc aaaacgcctg
     5581 gcgtaccgtg ggcggcacat ctgcgagttt gacctcgatg ccgccctcgc ccgccgtccg
     5641 gcgctgatct taatggacga actggcgcac agtaatgcgc caggttctcg tcaccctaaa
     5701 cgctggcagg acatcgaaga gctgctggaa gcaggcattg atgttttcac taccgtcaac
     5761 gttcagcatc tggaaagtct gaatgatgtg gtcagcggcg tcaccggaat tcaggtacgg
     5821 gaaaccgtgc ccgatccttt tttcgatgcc gccgacgacg tggttctggt ggacttgccc
     5881 ccggacgatc tgcgccagcg gctgaaagaa ggcaaagtct atattgccgg gcaggcggag
     5941 cgcgccattg aacatttttt ccgcaaaggc aatctgatcg ccctgcgcga actggcactg
     6001 cgccgtactg ccgatcgcgt tgatgagcaa atgcgcgcct ggcgggggca tcctagcgaa
     6061 gagaaagtct ggcacacgcg cgacgcgatc cttttatgca tcggccataa caccggtagt
     6121 gagaagctgg tccgcgcggc ggcgcggctg gcgtcacggc tgggtagcgt ctggcacgcg
     6181 gtgtatgttg aaacccctgc cctgcatcgc ttaccggaga aaaaacgtcg tgcaattctc
     6241 agcgccttac gtctggcgca ggaactgggc gcggagacgg caacactttc tgatccagcg
     6301 gaagagaaag cggtagtgcg ttatgcccgt gaacataatc tcggcaagat tattctcggt
     6361 cgcccggcct cgcgccgctg gtggcgtcgg gaaacgtttg ctgaccgact ggcgcgcatc
     6421 gcccccgatc tcgatcaggt gctggtcgcg cttgatgaac cacccgcccg cacgattaac
     6481 aacgcgccgg atagccgctc ttttaaagac aagtggcggg tacagattca gggatgcgtg
     6541 gttgccgccg cgttatgcgc cgttatcacc ttaattgcca tgcagtggct gatggcgttt
     6601 gatgccgcca acctggtgat gctgtatctg cttggcgtgg tggtggtggc gctattttat
     6661 ggacgctggc cttcggtggt tgccaccgtc attaatgtgg tgagtttcga tctctttttt
     6721 atcgccccac gcggcacgct cgccgtctct gatgtgcaat atctgctgac cttcgcggtg
     6781 atgttaaccg tcgggctggt gatcgggaac cttactgctg gcgtgcgtta tcaggcgcgg
     6841 gtagcccgtt accgcgagca acgcacacgg cacttatatg aaatgtcgaa agctctggcg
     6901 gtgggccgca gtccgcagga catcgccgcc accagcgaac aatttattgc ctccacgttt
     6961 catgcccgca gtcaggtgtt gttgcccgat gacaacggta aattgcagcc gttaacacat
     7021 ccgcaaggaa tgacgccatg ggacgatgcc atcgcgcagt ggagttttga taaaggcctg
     7081 cctgcgggcg cgggcaccga cacgttaccc ggtgtaccgt accagatttt gccgctaaaa
     7141 agcggcgaga aaacctacgg gctggtggtg gtggagccgg ggaatctgcg ccagttgatg
     7201 atcccggaac agcagcgcct gctggagacg tttacgctgt tagtcgccaa tgcccttgag
     7261 cggctgacgc taaccgccag cgaagaacag gcgcggatgg caagcgaacg tgaacagatc
     7321 cgcaacgccc tgctggcagc gctttcgcat gatttacgca cgccgcttac ggtgctgttt
     7381 ggtcaggcag aaatcttaac gctcgatctg gcaagcgaag gatcacccca cgcccgccag
     7441 gccagcgaga ttcgtcagca tgtgctgaac actacccgac tggtgaataa tctactggat
     7501 atggcgcgaa ttcagtccgg cggctttaat ttgaagaaag agtggttaac gctggaagaa
     7561 gtagtcggca gcgcgctgca aatgctggaa ccgggtttat cgtcgcccat caatctttct
     7621 ctgccagaac cgctgacctt aatccacgtt gacgggccac tctttgaacg ggtgctgatt
     7681 aatctgctgg agaacgcggt gaaatatgcg ggtgcgcagg ccgaaattgg tatcgatgcc
     7741 cactttgagg gcgaaaatct acaactggat gtctgggata acggccccgg tcttccgcca
     7801 ggccaggagc agacgatatt tgataagttt gctcgcggga ataaagagtc ggcggtgccg
     7861 ggtgtcgggc ttgggttggc aatttgtcgg gcgattgtgg atgtacacgg cggcaccatt
     7921 accgcgttca accgaccgga aggtggtgcc tgttttcgtg ttacacttcc ccagcaaacc
     7981 gctcctgaac ttgaagactt tcatgaggat atgtgacaaa cgttctgatt gttgaagatg
     8041 aacaggctat tcgtcgcttt ctgcgcacgg cgctggaggg cgacgggatg cgcgtctttg
     8101 aggccgaaac tctgcaacgc ggcttgctgg aagcggcaac ccgtaagcca gatttgatta
     8161 ttctcgatct cggcctgccc gatggtgatg ggattgagtt tatccgcgac ctgcgccagt
     8221 ggagcccggt gccggtgatt gtgctttccg cacgcagcga agagagcgac aagatcgccg
     8281 cgctggatgc cggagcggat gattatctga gtaagccgtt tggcattggc gaattgcagg
     8341 cccgtctgcg cgtcgcttta cgccgccact ccgccaccgc cgcgcccgat ccgctggtga
     8401 aattttccga tgttaccgtc gatttagccg cccgcgtgat tcaccggggt gaggaagagg
     8461 tgcatctcac accaattgag ttccgcctgc tggcggtgct gctcaacaat gccggaaaag
     8521 tactcaccca gcgccagctc cttaaccagg tgtgggggcc aaacgcggtc gaacacagtc
     8581 actatttgcg tatttatatg ggacatctgc gacaaaaact ggaacaggat ccggcccggc
     8641 ctcgccactt cattactgaa accggtattg gatatcggtt tatgctttga attgttattc
     8701 aataaattcg cttaaatagt gattaattta ttattaaacc tgcgccgaat attattattt
     8761 ggcaggttta atttcttaac gcaaatataa aacacgaaat tacaacattt aagtaacaaa
     8821 acagcatcta atatggcgat attcataatt aatgatttat tatctggggt atgatcgttt
     8881 tttgttgatc ttcttcacag attatagcca tttcatggat agaataactc taccttcaac
     8941 tgacacagca agaggtaaag gtaaatggaa aataataacc gcttaatgcc tcatataagg
     9001 cggacaaccc atatcatgaa gtttgcccat cgtaatagtt ttgactttca tttctttaat
     9061 gcccgctagt cttctgacta aagggcaccc caacgtacag gtctccctga ctttaagcat
     9121 tacaggttaa tacctgtatt cctcggtgct catatactgc taaccctttt aaactctaaa
     9181 taattcgagt cgcagcactt gcaacttgag gtatgacgag tatagccagt taccgggctg
     9241 gtctgggtta ttgcatctgc aaaaagcaaa ctactgattt atttatcagc ggtggagctt
     9301 tgcttttttt ccggcgtgat cgatttctcc tttgagaaat tgaggacctg ctattacctg
     9361 aaataaagag atgaataaaa tgtcagaatt aaaaattgcc gttagccgtt cttgcccgga
     9421 ttgtttttcc actcatcgtg catgcgtgaa tattgacgaa agtaattata ttgacgttgc
     9481 cgccattatt ttatcagtca atgatgttga acgtggaaaa ctcgatgaaa tagacgctac
     9541 tggctatggc attcctgttt ttattgcaac ggaaaatgaa gaacgtgtac cagcagaata
     9601 tctgccacgt atttctggtg tcttcgagca ttgcgaatca cggaaagagt tttatggacg
     9661 tcagttagaa accgctgcta gccattatga aacgcaactg cgcccgccct tcttccgcgc
     9721 actggtcgat tatgtcaatc agggcaatag cgcttttgac tgcccgggtc atcagggagg
     9781 tgagtttttc cgtcgtcatc cggcagggaa tcagttcgtg gagtacttcg gagaaacgct
     9841 gttccgttcc gacttatgca acgcagacgt agcaatgggc gatctgctga ttcacgaagg
     9901 cgcgccatgc attgcacagc aacatgcggc aaaagtgttt aatgccgata aaacatactt
     9961 cgttctaaat ggcacttcat cttctaacaa agtggtttta aacgccctgc taacaccggg
    10021 tgatctggtg ctgtttgatc gcaataacca caaatctaac caccacggag cgttgctaca
    10081 ggctggtgca acaccggttt atctggaaac ggcacgtaac ccgtatggct ttatcggtgg
    10141 cattgatgcg cactgttttg aagaaagtta cctgcgtgag ctgatcgcgg aagtcgcacc
    10201 gcagcgggca aaagaggctc gtcctttccg cctcgctgtg attcagttag gcacctacga
    10261 cggtacgatt tataacgccc gccaggtggt ggataaaatt ggtcatctgt gtgactacat
    10321 tctgtttgac tccgcatggg ttggctatga gcaatttatc ccgatgatgg ctgactgttc
    10381 gccgctgttg ctggatctta atgagaacga tccgggtatt ctggttacgc aatccgtaca
    10441 taaacagcag gctggttttt ctcaaacttc acaaatccat aaaaaagaca gccacatcaa
    10501 agggcaacag cgttatgtac cgcacaaacg catgaacaac gcctttatga tgcacgcctc
    10561 caccagcccg ttctatccgc tgtttgccgc actggatatc aacgccaaaa tgcatgaagg
    10621 tgtcagcggt cgtaatatgt ggatggattg tgtggtaaat ggcattaatg cccgcaaact
    10681 gatcctcgat aactgtcagc atattcgtcc gttcgtacct gaactggtgg atggtaaacc
    10741 ctggcagtcg tatgaaacag cgcaaattgc ggttgatctg cgcttcttcc agtttgtacc
    10801 aggggaacac tggcattctt ttgaaggcta tgcagagaat caatactttg tcgatccttg
    10861 caaactgttg ctgacaaccc caggtattga tgcacgtaac ggcgaatatg aagcgttcgg
    10921 tgtacccgcg acgattcttg ctaacttcct gcgcgaaaat ggcgtagtgc cggaaaaatg
    10981 cgatcttaac tccatcctct tcctgctgac tccggcagaa gatatggcca aacttcagca
    11041 acttgttgcc ctgctggtac gcttcgaaaa actgcttgag tccgacgcgc cattagcaga
    11101 agtgctgcct tccatctaca aacagcatga agagcgctac gccggttata ccctgcgtca
    11161 gttgtgccag gaaatgcatg atttgtatgc ccgccacaac gtgaaacaac tgcaaaaaga
    11221 gatgttccgc aaagcgcact tcccacacgt cagcatgaat ccgcaagaag ccaactacgc
    11281 ctatttacgc ggtgaagtgg agctggttcg tctgccggat gccgaaggtc gtatcgctgc
    11341 cgaaggggcg cttccttatc ctccgggtgt gctgtgtgtt gttccgggtg aaatctgggg
    11401 tggtgctgtt ttgcgttact tcagcgctct ggaagaaggg atcaacctgc tgccaggttt
    11461 tgcaccggag ctgcagggtg tctatatcga agaacatgat ggtcgtaagc aagtttggtg
    11521 ctatgtcatc aagcctcgtg atgcgcaaag caccctgttg aaaggggaaa aattatgagt
    11581 caggctaaat cgaacaaaat gggcgtcgtt cagttaacca tactgacgat ggtcaacatg
    11641 atgggctccg gtatcatcat gctgccgaca aagcttgccg aagttgggac aatttcaatt
    11701 atctcctggc tggtgacagc cgtaggctca atggcactgg catgggcatt cgcaaaatgc
    11761 ggtatgttca gccgtaaatc aggtggtatg ggcggttatg ccgagtatgc tttcggtaaa
    11821 tccggtaact ttatggcgaa ctatacctac ggcgtctcac tgctgattgc taacgtcgcg
    11881 attgctattt cggcggttgg ttacggcacc gaactgctcg gcgcgagttt gtcgccagtg
    11941 cagattggtc ttgcgaccat cggggtgctg tggatttgta ccgtggctaa ctttggtggt
    12001 gcgcgcatta ccgggcaaat cagtagcatt accgtgtggg gggtcattat tccggtggta
    12061 ggcctgtgca ttatcggctg gttctggttt agcccgacgc tttacgttga ttcctggaat
    12121 ccgcatcatg caccgttctt cagtgcagta ggttcttcca tcgctatgac gctgtgggct
    12181 tttctcggtc tggagtctgc gtgtgcgaat actgatgtag tggaaaaccc ggaacgtaat
    12241 gtgccaatcg cggtactcgg cggtacgtta ggtgcggcgg tgatttatat cgtctccacc
    12301 aacgtgattg cagggattgt gcctaatatg gagctggcaa attcaacggc accatttggc
    12361 ctggccttcg cacagatgtt cacaccagaa gtgggtaaag tcattatggc gctgatggtg
    12421 atgtcctgct gcggttcgct gcttggctgg cagttcacca ttgcccaggt gtttaagtct
    12481 tcatctgatg aaggctactt ccctaaaatt ttctcccgtg taaccaaagt ggatgcaccg
    12541 gtgcagggaa tgttgaccat tgtgattatc cagagcgggt tggcgctgat gacgattagc
    12601 ccgtcgctaa acagtcagtt caacgtgctg gttaacctgg ccgtggtgac caatatcatt
    12661 ccgtatattc tgtcaatggc ggcattagtc attattcaga aggtcgctaa tgtgcccccc
    12721 tcaaaagcga aagttgcaaa ctttgttgct tttgttggcg cgatgtatag cttttatgcg
    12781 ctgtactcat ccggggaaga agccatgctg tacggttcca tcgtgacctt ccttggttgg
    12841 acactgtatg gtctggtatc accacgcttt gaactgaaaa ataaacacgg ttaaaaaagc
    12901 aaagtaaaaa agggcgatct tgcgaccgcc ctttttttat taaatgtgtt tacgcgtttt
    12961 tcagaacttc gctaacaatc tcaaccgctt ctttctcaat ctgcttgcga tgttcttcac
    13021 cgaggaagct ttcgcagtag atcttatatg cgtcttccgt gcctgacgga cgcgcggcga
    13081 accagccgtt gtcagtcatc actttcagac cgccgataga agcaccattg cccggtgccg
    13141 ccgtcaggcg cgcggtgatc gggtcgcctg ccagggtgct ggcgctcacc atttccggag
    13201 acagcttaga cagcgccgct ttttgtgcgg aagtcgcagc tgcctgcaaa cggttgtagc
    13261 tcggcgcacc aaagcgtttt gccagttcgt tgtagtgttc ctgcgggttc ttaccggtga
    13321 cagcggtgat ttccgccgcc agcagacaca tgatgatgcc gtctttgtcg gtggaccacg
    13381 gcgtgccgtc gaaacgcagg aaggaagccc ctgcgctctc ttcgccgcca aagccgaagc
    13441 tgccgtcgaa cagaccatca acaaaccatt tgaaacctac cgggacttct accagcttgc
    13501 ggcccaagtc attgaccaca cggtcgatca tcgcagaaga aaccagcgtt ttaccgacag
    13561 caacatcttt gccccactgc ggacgatgct ggaacaggta gttgatcgcc accgccaggt
    13621 agtggttcgg attcatcaaa cctgccggag tgacgatacc gtgacggtca taatccgggt
    13681 cgttagcaaa cgccagatcg aacttatcac gcaatgccag caggcccgcc atcgcacact
    13741 cggaggagca gtccatacgg atcgcgccgt ctttatcgag gtgcataaag cggaaggttt
    13801 gatcgacctg atcgttaacg atggtcaggt tgaggttgta atactcgcca attcgcttcc
    13861 agtattcgat accggaacca cccagcggat caacgcccag cgtcaggccc gttttctgaa
    13921 tcgccgccat atcaacgata tcggccagac cttccacaaa ctgctgcacc agatcctgct
    13981 ctttcacatg accggatgcc atcgcttcgt cgagggagat acgcttcacg cctttcaggc
    14041 catcggccat cagtgcgttg gcacgatctt ccaccacttt agtgacgttg gtatcagccg
    14101 ggccaccatt tggcggattg tacttgatac caccatcttc cggcgggtta tgggagggtg
    14161 taatcacgat accgtctgcc agcgggccac cttttttatt atgaaccagg atggcattgg
    14221 aaacggcagg cgtcggagtg aagccattgt tttcctgcac aatgacatca acgccgttcg
    14281 ctgccagcac ttccagtacg gaaatgaacg caggctcgga cagggcgtga gtatctttac
    14341 ccacatagca agggccagtg atgccgtttt tcgcacgttc ttcagcaatt gcctgagcga
    14401 tcgccagaat gtgcggctcg ttaaagctgt ggcgcgctgc actgccacgg tgaccggaag
    14461 taccgaattt caccgcgtgc tccgcattcc ctgcttctgg tttcagtaca taatattgcg
    14521 ccgtcagttg ggcgacgtta atcaaatcac tctgttgtgc aggttggcct gcacgattgt
    14581 ggattgccat tgctttgtcc tttgtctgca acgttttaga tagttccgca aaccttctca
    14641 atcaattccg ccgggaattg catcgactgc atgatgtgtt cgatcatgct gcatttacgg
    14701 ccggtgttgg tgttggtgat cacccaatac ggcgtgcctg gcacatgttt cggcttggtc
    14761 tgattaccat ttttcagcag cgtttgttca tctgccgcaa agtaaacgcg tgtacgaccg
    14821 tgcaacgatt ccgttgcttc ggcaaacgcc tgggcgtcaa gagaatatag tgtagacaac
    14881 agcagcataa agcgattgac cgctcgcttt tgctctgcgt attcgtcaga aagcagaagt
    14941 tcacgcattg cgcgaacctt gtctttaatc gttttgaccg gcttcgcttc gacgatagca
    15001 ggtgacgcaa cgcgaacctc tttcgtcacc ggagcagcag gctgtgatgc ggcggaaaat
    15061 ttcaacatac gccgtaaaat gtcggatgcg ctctcgccga tatgcttagt gtggctggca
    15121 atatagctgt agagttcatc atcaacttca atcgttttca tcttaatcca gtacagtgtc
    15181 ttagctgaat acaagtcgcc aagagtatat gaataaatcc cggcagcgga tagcgtcaag
    15241 cctggcttgc agaaaaactc ggaataaacc ttatttgttg cagccctgtg gcacaatggt
    15301 caacataata ccctaacccg acaacgcgaa aaaaagaact ttgccatgaa attgaatatc
    15361 cgcgcgcaaa ctgcacaaaa ccagcacaat aattctccca tcgttcttgt ccatggtctg
    15421 tttggtagcc tcgacaacct tggcgtactg gctcgcgatc tggtaaacga tcacaatatc
    15481 atccaggttg atatgcgtaa ccacggtctt tcaccgagag atccggtaat gaattacccg
    15541 gcgatggcgc aggatcttgt tgatactctg gatgcacagc agatcgacaa agcaacattt
    15601 atcggtcact ccatgggcgg taaagcggta atggcactta cggcactggc ccgcgatcgc
    15661 atcgataaac tggtggcgat cgatatcgca ccggtcgact atcacgtacg ccgtcatgat
    15721 gagattttcg cggctatcaa cgcggtcagt gaatcggacg cacaaactcg ccagcaagca
    15781 gcagcaataa tgcaccagca tcttaatgaa gaaggggtga ttcagtttct gttgaaatct
    15841 tttgttgacg gggagtggcg ctttaacgtg ccggtattgt gggatcagta tccgcatatt
    15901 gtgggttggg agaaaatccc ggcatgggat caccctgccc tgtttatccc tggcggcaat
    15961 tctccgtatg ttagcgagca gtaccgtgat gatttactgg cacaatttcc acaggcacgg
    16021 gcgcatgtga ttgcaggcgc gggtcactgg gtccatgctg aaaaaccgga tgcggtatta
    16081 cgcgctatcc gtcgctatct caatgattaa cgcaactgat taaaaaccca gcgtccgcgt
    16141 cgctttccgc gcgtgggcgt tggcgtgact catcgcgctg atgtattatg gcgcgctatc
    16201 catccgggca gaggcctggc tgattgtttc ccccgaagtc accaagatca tggccaaaga
    16261 acaaacggac cgtacgacat tagatctgtt cgcgcacgag cgtcgaccgg gacgaccgaa
    16321 aactaatccg ctttcgcgcg atgaacagct gcgtattaat aaacgcaacc agctaaaacg
    16381 cgacaaagta cgtggcctta agcgtgtcga actgaagctg aacgcggaag ctgtcgaggc
    16441 gctgaacgag ctggcggagt cgcgcaatat gagccgtagc gaactgatcg aagagatgtt
    16501 aatgcagcaa ctggcggcgc tgcgtagtca gggcatcgtt taaatttcca ctttcatgta
    16561 gcacagtgtg cagtcctgct cgtttgctga ttccgcaaga ctgcctgttc tgctatgatt
    16621 gcctttatcc gtgggcaatt ttccaccccc atttcaataa gtttcaagag gttatttcac
    16681 tcatggctat cactggcatc tttttcggca gcgacaccgg taataccgaa aatatcgcaa
    16741 aaatgattca aaaacagctt ggtaaagacg ttgccgatgt ccatgacatt gcaaaaagca
    16801 gcaaagaaga tctggaagct tatgacattc tgctgctggg catcccaacc tggtattatg
    16861 gcgaagcgca gtgtgactgg gatgacttct tcccgactct cgaagagatt gatttcaacg
    16921 gcaaactggt tgcgctgttt ggttgtggtg accaggaaga ttacgccgaa tatttctgcg
    16981 acgcattggg caccatccgc gacatcattg aaccgcgcgg tgcaacgatc gttggtcact
    17041 ggccaactgc gggctatcat ttcgaagcat caaaaggtct ggctgatgac gaccactttg
    17101 tcggtctggc tatcgacgaa gaccgtcagc cggaactgac cgctgaacgt gtagaaaaat
    17161 gggttaaaca gatttctgaa gagttgcatc tcgacgaaat tctcaatgcc tgatgtgatg
    17221 cggcgtagac tcatgtctac gccgtattaa tagataatgc caatcaaaat aattgctaca
    17281 aatttgtaac ttttgctgtt gtacctgtac aatgtcccgg tgttcaagtg gccttgccgt
    17341 tgtaaatgta agctgtgcca cgtttttatt aacaatattt gccagggact tgtggttttc
    17401 atttaggcgt ggcaattcta taatgatacg cattatctca agagcaaatt ctgtcacttc
    17461 ttctaatgaa gtgaaccgct tagtaacagg acagattccg catgactgat aacaataccg
    17521 ccctaaagaa agctggcctg aaagtaacgc ttcctcgttt aaaaatccta gaagttcttc
    17581 aggagccgga caaccatcac gtcagtgcgg aagatttata caaacgtctg atcgatatgg
    17641 gtgaagaaat tggtctggct acggtatatc gcgtactgaa ccagtttgac gacgctggta
    17701 tcgtcacccg ccacaatttt gaaggcggta aatccgtatt tgaactgaca cagcaacatc
    17761 accacgatca cctgatctgc ctcgactgcg gcaaggttat cgaatttagt gatgattcca
    17821 tcgaagcgcg tcagcgtgaa attgccgcaa aacatggcat tcgcctgact aaccacagtc
    17881 tctatcttta cggtcactgt gccgaaggcg attgccgcga agatgaacat gcgcacgaag
    17941 gcaaataagc cagcctgaat gagaaaaagc caacctgcgg gttggctttt ttatgcaagg
    18001 gaaataagaa aagcgagagt tacagctctc acttatttgt tattactgcg aatttcctgc
    18061 caaactttat cacaatcggc tttcaccgcc tgatcatttc cggtttgcgt tgcacgcaga
    18121 tactgctgat aatccagtac gcggacactt tcctggtcgg caaaatgctg atgttgtttc
    18181 tctttcttca gcacgtttaa cacgctctgg caggcttcaa ttttttctgg cgaaccttgt
    18241 gcggtgttga tacaggcgct gtatgcctct ttcagacgac tgtcttcctg tggcgcggtt
    18301 gattgcgcac aagctaccag ccccgatgcc attatggcga tgagaatcag ttttttcata
    18361 gcaaattcct caaacgtgcg gcaggcacaa aacctgccgc gtcgggcatc agaagatggt
    18421 gaatggtgcg attaccataa atttcacgtc acgctcatcc tggaagatgt tgccgtaacc
    18481 accgccccag cttgggatgt cggagtggtt gtcgtattcg gtgaagtgca gtttgaacat
    18541 cgtgcctttg gcgcgaccgt cctgaatggt gtagaccgca tccaggctgt atgcagactc
    18601 ttcaatagtc cggtttttgt cgtagtacgc atccggattg ctctgctagg tcgcaggttt
    18661 agcatcccat gcgtaaacgt aggaagcgcc gatggcgaag cctggaagat tccagttttt
    18721 caggtcatac atcgcaccga agaagaccgc tttttcgccg ttagcgttga agtcggaacg
    18781 gttatcccac cagatatcca ggcggccgtt ggaggaagcg taggttggag tcatacgttg
    18841 caggaagtat ccctgctgac cgtcagcctt aacccaggtg ccttcgaggc gcaaatccac
    18901 tacgtcagcc gcccggtaac caaaggtcaa cgcttgcagc caggcggtgc cgtcataaag
    18961 gtcgttgacg ctgcgatcgt caactttatc gcgcgtacca tagaactggt agctggtggt
    19021 taacgggcta ccggcgatat caaatttgta gctggctttg gcaaaatatt gatcgatata
    19081 cccttccgcc tgaccaaatg ccgcttccag tacgaagtta tttttgaagt cgtatttcgc
    19141 cccaagggag tgcagataat caactttggt ggttttatcg ttctgataaa actcatccat
    19201 ttccagatgc cacggcgctt tgtattcgtt ggtccacatg taggagaaac tcaacgcacc
    19261 agcatcgccg taatcaaaat tcgccccggc ttccgccccc tgataagtac ctggcataaa
    19321 gctccagtgc ggcgctaaca gcgtttgacc agttggctga atgtaacctg ccctcgccca
    19381 aaccggaccg tatttaaatt tggccgcagc tttatacagg cttataccgc ttttgtcgcc
    19441 ggaccagtct tcgtcatagg ctttattact ttttgaaaac gcgatttcgt tcgggtggga
    19501 gctgtcgccg ttttccgcca tttcaatcgc cgtaaacgca gcaatatcaa ggccgaacat
    19561 atcagcagca taaccggact gaaaatcgag gttggcattc caggtggagt gagaaaggtt
    19621 ggttttgtat ttgtcgccgt cggtaacatc tttacggtcg cgttcacgct gccagtaata
    19681 tataccgccg gttaaggttg aatcgtcgat gaatccttcg gcgcgagcct ccggcattgc
    19741 cataaagccc gacattgctg taacaccggc gatagccagc gccagcgtac tacgtttgcc
    19801 actaaacgta cgcatgggtt aatcctcttt gacgtataaa ttgctgcacc aaaggtgaac
    19861 agcgaaaaaa taaaaattaa aaaagtagcg ttgagaaaag tctcgctgac tacaggaaca
    19921 gactattttt cgcgacgcga attatcaatc tctttctgta accagaatgt aagaacatga
    19981 gcaaccgcac atattttgat gatttttgtt acaaggctta agtttgcggt tgaaaggctt
    20041 gcagaagaat aaaagcgtga aattccagca agatgaaagc ggttacattt tatttaatta
    20101 tgttttgtta ttttaaattt attttatgga taaattgcga agcggaatta attcgcgaag
    20161 aaaagcagaa aaaacgccgc tgaacgcggc gttttggatt gtttgcatat ttcttgccgg
    20221 atgcggcgtg aacgccttaa ccggcctaca aaagcacgca aactcaatat attgcagaga
    20281 tcatgtaggc ctgataagcg tagtgcatca ggcaatttag cgtttgaaac tgtttgtctg
    20341 ttccttgccg gatgcgacgt aaacgcccca tccggcatag cgaaacttaa aattactcgc
    20401 ctactttcgc ccaggtatca cgcagcccaa cggtgcggtt aaataccggt ttttccgccg
    20461 tagaatggcg gctatcgagg cagaagtaac cttcacgctc aaactggaat gctttacccg
    20521 caaccgcatc tttcagcgac ggttcagcaa agccctgttt gatcaccagc gattccgggt
    20581 taatcaccga caggaaatca tccgcagcac cagggttcgg cacgctgaac agacggtcgt
    20641 acagacggat ttcaaccggc agcgcatgtg ccgcgctcac ccagtgaata acacctttga
    20701 ctttacgacc atctgccgga tctttgctta aggtgtcggc gtcataagta cagaagatgg
    20761 tggtgatatt gccttcggca tctttctcca cgcgttctgc cttaatgacg taagcattac
    20821 gcagacgcac ttctttaccc agcaccagac gtttgtactg cttgttagct tcttcgcgga
    20881 aatcggcgcg atcgatccaa atctcaccgc taaacggcac ctggcggcta cccatttccg
    20941 gtttgttcgg atggttcggc atggtaacca tttcgccttc gccctgatag ttttcgataa
    21001 ccagtttcac cggatcgata accgccattg cgcgcggggc gttttcgttg agatcttcac
    21061 ggatgcagga ttccagcgac gccatctcaa tggtgttgtc ctgcttggtc acgccgatgc
    21121 gtttgcagaa ctcacgaata gaagcagcag tgtaaccacg acgacgcaga ccggaaatgg
    21181 tcggcatacg cgggtcatcc cagccttcaa cgtgcttgtc ggtcaccagc aggttcagct
    21241 tacgcttgga catcacagtg tattccagat tcaggcgcga gaattcgtac tggcgcgggt
    21301 gaacaggaat ggtgatgttg tccagcaccc agtcgtacag gcgacggttg tcctggaact
    21361 caagcgtaca cagagagtgc gtaatacctt ccagggcatc gctgatgcag tgggtgaagt
    21421 cgtacatcgg gtagatgcac cacttgttgc cagtctggtg gtgttcagca aatttaatgc
    21481 ggtacagcac cggatcgcgc atcacgataa acggtgaagc catgtcgatt ttcgcacgca
    21541 ggcaggcttt accttcttca aaaccaccgg cacgcatttt ttcgaacagc gccaggttct
    21601 cttcaacgct gcggtcgcgg tacgggctgt ttttacccgg ttgcgtcagg gtgccgcggt
    21661 attcgcggat ctgttccggc gtcagttcat caacgtacgc caggccttta ttgatcagtt
    21721 cgatcgcata ggcgtggagc tgatcaaaat aatcggagga gtaacggacg ttaccagacc
    21781 agtgaaaacc taaccactct acgtcgtttt tgatcgactc aacatactcg atatcttctt
    21841 ttaccgggtt agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc tgggcgatcc
    21901 cgaagttcag gcagatagat ttcgcatggc caatatgcag atagccattc ggctccggcg
    21961 ggaaacgggt gtgtactgtg gtgtgcttac cactggccag atcttcatcg atgatctgac
    22021 ggataaagtt agtcgggcgg gcttctgcct cactcatcgt ggattcctca aagcgtaaac
    22081 aacgtataac ggcgtatgat cttataagcg ggacaggctg acaactgtta gttagcgaaa
    22141 atcgaaaatc tcactgagat tatggggatg attgatgaaa aaaaaaagcg gcagagaaga
    22201 tctccaccgc ttttgccagt cccgcgtcat tgttggatgc gcgtcttatc caaccaggac
    22261 tgagcttttg taggcctgat aagacgcgtc aagcgtcgca tcaggcataa agcagattac
    22321 tttttgattt catacagcgg tgtttgaccc gccacaacat tgccctgagc tttaatgatc
    22381 aagccactga aatcgtcgat attgctgcaa accaccgggc taatcatcga acgggcgtta
    22441 gcgttcaggt aatccagatc catttccaga atcggttgcc ctgcgctcac ctgcgcgccc
    22501 tcttccacca gacgtttaaa gcctttacct tccagcgcta cggtgtcgat acccatgtgg
    22561 acgacaatct ccgcgccttt ttcggtttct aggcagaacg cgtggttggt gttgaagatt
    22621 ttcacgattg tccctgcggc tggtgatacg acgattttat ctgtcggttt caccgccaca
    22681 ccgtcaccca ccgctttgct ggcgaatgct tcgtcagaaa cctgatccag tgccacgaca
    22741 tcaccggtaa tcggcgatac cagctccgcg atagataccg cgtttggtac agcctgcggt
    22801 tttgctacag gagcggcagt tgccggagtt gcttcagctg acgcagctgc taccgggcca
    22861 cgggcaacga ctttcttcat cgcatcgcca atggattctg ctttcgcgcc aacaatcacc
    22921 tgaatggtct gtttgttcag ttttactaca ccagaagcac ccagacgttt acacatcgca
    22981 tcgttgacgc gagctgagtc agccacggta aggcgcagac gggtaataca ggcgtcaatc
    23041 gctttcaggt tgtcagtacc gccaaccgca gcaatatagt tggttgccag ttgagtcaga
    23101 ccttcttcag tattgctgtt agcctcttca gttacgatct cgtcttcttt atcttcacga
    23161 cccggcgttt tcaggttgaa catgcggata accaaactga acaccacgaa gtagatagcg
    23221 aagaagacaa cacccatcac cagcagcatc cagacgttct ggctggcggc cggcaggtta
    23281 tacatcaacg cgtagtcgat agcccccgca gagaaagaga agccagcatg gatacccagc
    23341 agcgttgcca caaacaggct gataccggtc agcagtgcgt gcaggaggta cagcagcgga
    23401 gcaaggaaca tgaacaggaa ttccagcggc tcagtcacac cggtcaggaa cgcagtaaca
    23461 gcaacagaaa gcagcatccc gccaaccatc ggacgacgct ctttcggtgc tgcgaagtac
    23521 atcgccagcg ccgcacccgg cagaccgaac atcatgatcg ggaagaagcc ggacatgaac
    23581 atccccgcgg tgccgtcacc agcgtagaaa cggttgatgt cgccgtggaa aaccgtaccc
    23641 gccgcgttgg tgaattcacc aatctggaac caggcgatgg tgttcagcac ctgatgcaga
    23701 ccggttggga tcagcagacg gttgatgaaa ccaaagatac cggaacccag cgcgcctgca
    23761 gaaacgatcc actcgccgcc tgcatggata gcgtgctgta ctggcggcca gacgtaacca
    23821 aaaatggccg ccagcaccag gcagaaaaag ccggtggcaa tcggcacaaa gcgtttgccg
    23881 ccgaagaagc tcaggaagtc cggcagttta atatcggacc aacggttata ggctgcgcca
    23941 ccaaccagac cggtaatgat acccgccagt acacccatgt taatttctgg gttgatggtc
    24001 accatcgctt tggttaacac aaagtaacct accgcacccg ccagcgccgc cgcacctgcg
    24061 ctgtctttcg accagctgga tgccacaccg atggcgaaga ttaatgcgag gttatcaaaa
    24121 atcgcaccgc ccgcctgggc aataaacgca acgttaagta aatctggctg accgaatcgc
    24181 agcaacagtg ccgccaccgg cagcaccgcg atagggagct gtaacgccct accgagtcgc
    24241 tggaaaaaac ctaaaatatt catcttattc cccctacgag aaccctattt ggctcgttta
    24301 aagccgtatt tttattttgc tgcaaattgt actgccgatg ttctgtaatc agattgttag
    24361 atcatctgct acagagtgtg tgaaaattta attcgtatcg caaattaaac gcgtgccttt
    24421 tgtgagtttt gtcaccaaat atcgttatca tcactccctt ttactggcta aagcagaaaa
    24481 cttattttat cattcaaaaa atcaggtcgg attgacgcct gtctgcgcaa atccaggtta
    24541 cgcttaaaga tgcctaatcc gccaacggct tacattttac ttattgaggt gaataatgag
    24601 actgatcccc ctaactaccg ctgaacaggt cggcaaatgg gctgctcgcc atatcgtcaa
    24661 tcgtatcaat gcgttcaaac cgactgccga tcgtccgttt gttctgggcc tgccgactgg
    24721 cggcacgccg atgaccacct ataaagcgtt agtcgaaatg cataaagcag gccaggtcag
    24781 ctttaagcac gttgtcacct tcaacatgga cgaatatgtc ggtctgccga aagagcatcc
    24841 ggaaagctac tacagcttta tgcaccgtaa tttcttcgat cacgttgata ttccagcaga
    24901 aaacatcaac cttctcaacg gcaacgcccc ggatatcgac gccgaatgcc gccagtatga
    24961 agaaaaaatc cgttcttacg gaaaaattca tctgtttatg ggcggtgtag gtaacgacgg
    25021 tcatattgca tttaacgaac cggcgtcttc tctggcttct cgtactcgta tcaaaaccct
    25081 gactcatgac actcgcgtcg cgaactctcg tttctttgat aacgatgtca atcaggtgcc
    25141 aaaatatgcc ctgactgtcg gtgttggtac attgctggat gccgaagaag tgatgattct
    25201 ggtgctgggt agccagaaag cactggcact gcaggctgca gttgaaggtt gcgtgaacca
    25261 catgtggacc atcagctgtc tgcaactgca tccaaaagcg atcatggtgt gcgatgaacc
    25321 ttccaccatg gagctgaaag ttaagacttt aagatatttc aatgaattag aagcagaaaa
    25381 tatcaaaggt ctgtaattgt tatccctgcc ctggctcctt gctcagggca atatttttta
    25441 aaatcggggg gcagaatgta tgcattaacc cagggccgga tctttaccgg ccacgaattt
    25501 cttgatgacc acgcggttgt tatcgctgat ggcctgatta aaagcgtctg tccggtagcg
    25561 gaactgccgc cagagatcga acaacgttca ctgaacgggg ccattctctc ccccggtttt
    25621 atcgatgtgc agttaaacgg ctgcggcggc gtacagttta acgacaccgc tgaagcggtc
    25681 agcgtggaaa cgctggaaat catgcagaaa gccaatgaga aatcaggctg tactaactat
    25741 ctgccgacgc ttatcaccac cagcgatgag ctgatgaaac agggcgtgcg cgttatgcgc
    25801 gagtacctgg caagacatcc gaatcaggcg ttaggtctgc atctggaagg tccgtggctg
    25861 aatctggtaa aaaaaggcac ccataatccg aattttgtgc gtaagcctga tgccgcgctg
    25921 gtcgatttcc tgtgtgaaaa cgccgacgtc attaccaaag tgaccctggc accggaaatg
    25981 gttcctgcgg aagtcatcag caaactggca aatgccggga ttgtggtttc tgccggtcac
    26041 tccaacgcga cgttgaaaga agcgaaagcc ggtttccgcg cggggattac ctttgccacc
    26101 catctgtaca acgcgatgcc gtatattacc ggtcgtgaac cgggcctggc gggcgcgatc
    26161 ctcgacgaag ctgacattta ttgcggtatt atcgctgatg gcctgcatgt tgattacgcc
    26221 aacattcgta acgctaaacg ccttaaaggc gacaaactgt gtctggttac cgacgccacc
    26281 gcgccagcag gtgccaacat tgaacagttc atttttgcgg gtaaaacaat atactaccgt
    26341 aacggacttt gtgtggatga gaacggtacg ttaagcggtt catccttaac catgattgaa
    26401 ggcgtgcgta atctggtcga acattgcggt atcgcactgg atgaagtgct gcgtatggcg
    26461 acgctctatc cggcgcgtgc gattggcgtt gagaaacgtc tcggcacact cgccgcaggt
    26521 aaagtagcca acctgacctc attcacacct gattttaaaa tcaccaagac catcgttaac
    26581 ggtaacgagg tcgtaactca ataagagaaa gtatgacacc aggcggacaa gctcagatag
    26641 gtaatgttga tctcgtaaaa cagcttaaca gcgcggcggt ttatcgcctg attgaccagt
    26701 acgggccaat ctcgcggatt cagattgccg agcaaagcca gcttgccccc gccagcgtaa
    26761 ccaaaattac gcgtcagctt atcgaacgcg ggctgatcaa agaagttgat cagcaggcct
    26821 ccaccggggg ccgccgcgct atctccatcg tcaccgaaac ccgcaatttc cacgcaatcg
    26881 gcgtacggct tggtcgtcac gacgccacca tcaccctgtt tgatctcagc agcaaagtgc
    26941 tggcagaaga acattacccg ctgccggaac gtacccagca aacgctggaa catgccctgt
    27001 tgaatgccat tgctcagttt attgatagct accagcgcaa actgcgcgag ctgatcgcga
    27061 tttcggtgat cctgccaggg cttgttgacc cggacagcgg caaaattcat tacatgccgc
    27121 atattcaggt agaaaactgg gggctggtag aagctctgga agaacgtttt aaagtgacct
    27181 gtttcgttgg tcacgatatc cgtagtctgg cgctggcgga gcactacttc ggtgcaagtc
    27241 aggattgcga agactccatt ctggtgcgtg tccatcgcgg aaccggggcc gggattatct
    27301 ctaacgggcg catttttatt ggccgcaacg gtaacgtcgg tgaaattggc catattcagg
    27361 tcgaaccgct gggtgaacgc tgccactgcg gcaactttgg ctgcctggaa actatcgccg
    27421 ccaacgctgc cattgaacaa cgggtgttga atctgttaaa gcagggctac cagagccgcg
    27481 tgccgctgga cgactgcacc atcaaaacta tctgcaaagc cgcgaacaaa ggcgatagtc
    27541 tggcgtcgga agtaattgag tatgtcggtc gtcatctggg taaaaccatc gccattgcta
    27601 tcaacttatt taatccgcaa aaaattgtta ttgccggtga aatcaccgaa gccgataaag
    27661 tgctgctccc tgctattgaa agctgcatta atacccaggc gctgaaggcg tttcgtacta
    27721 atctgccggt ggtacgttct gagctggatc accgctcggc aatcggcgct tttgcgctgg
    27781 taaaacgcgc catgctcaac ggtattttgc tccagcattt gctggaaaat taatgtgctt
    27841 ttatagtggc gcttattgtt gtcaatattc tgggtagtcc atgaccatta aaaatgtaat
    27901 ttgcgatatc gacggcgtgc tgatgcacga taacgtcgcc gtaccgggtg cagcggaatt
    27961 tttgcacggg attatggata aaggcctgcc gctggtgttg ctgaccaact atccttcgca
    28021 gaccgggcaa gatctggcga accgctttgc cactgccggt gtcgatgtgc cggatagcgt
    28081 gttttatacc tctgcgatgg cgaccgccga tttcctgcgt cgccaggaag gcaagaaagc
    28141 atatgtggtg ggcgaaggcg cactgattca tgaactgtac aaagccggtt tcactattac
    28201 cgatgtgaac cctgatttcg tgattgttgg cgaaacgcgt tcctacaact gggacatgat
    28261 gcataaagcg gcctatttcg tcgctaacgg tgcacgcttt atcgccacca acccggacac
    28321 ccacgggcgc ggtttttatc ccgcttgtgg cgcgttgtgt gcagggattg agaaaatctc
    28381 cgggcgcaaa ccgttctatg ttggtaagcc cagcccgtgg atcatccgcg cagcattaaa
    28441 caaaatgcag gcgcattcgg aagaaacggt gattgtcggc gataacttgc gcaccgacat
    28501 tctggcgggc ttccaggcag gtctggaaac gattctggtg ctttctggtg tttcatcgct
    28561 cgacgatatc gacagtatgc cgttccgccc aagctggatt tacccgtcag tcgctgaaat
    28621 cgacgttatc tgaataccac ccacgtctgc ggacgtggtt ggctctgatg acaaacgcca
    28681 aactgcctga tgcgctacgc ttatcaggcc tacgcagctc ctgcaatata ttgaattttc
    28741 atgcttttgt aggccggata aggcgttcac gccgcatccg gcatgaacaa atcgcacttt
    28801 atcagcaatt tggc
//
LOCUS       DAETQN010000045        28797 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.045, whole genome shotgun sequence.
ACCESSION   DAETQN010000045 DAETQN010000000
VERSION     DAETQN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.045"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="K0W68_001971"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="K0W68_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBI3073534.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="K0W68_001972"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="K0W68_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBI3073535.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="K0W68_001973"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="K0W68_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBI3073536.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="K0W68_001974"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="K0W68_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBI3073537.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="K0W68_001975"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="K0W68_001975"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBI3073538.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="K0W68_001976"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="K0W68_001976"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBI3073539.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="K0W68_001977"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="K0W68_001977"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBI3073540.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="K0W68_001978"
     CDS             complement(4604..5326)
                     /locus_tag="K0W68_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3073541.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="K0W68_001979"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="K0W68_001979"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBI3073542.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="K0W68_001980"
     CDS             6526..6744
                     /locus_tag="K0W68_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBI3073543.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="K0W68_001981"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="K0W68_001981"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBI3073544.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="K0W68_001982"
     CDS             complement(7478..7678)
                     /locus_tag="K0W68_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBI3073545.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="K0W68_001983"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="K0W68_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HBI3073546.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="K0W68_001984"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="K0W68_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBI3073547.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="K0W68_001985"
     CDS             10175..12088
                     /locus_tag="K0W68_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3073548.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="K0W68_001986"
     CDS             12088..13110
                     /locus_tag="K0W68_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBI3073549.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="K0W68_001987"
     CDS             13104..13322
                     /locus_tag="K0W68_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBI3073550.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="K0W68_001988"
     CDS             13376..14245
                     /locus_tag="K0W68_001988"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBI3073551.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="K0W68_001989"
     CDS             complement(14300..14704)
                     /locus_tag="K0W68_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBI3073552.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="K0W68_001990"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="K0W68_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBI3073553.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15689..17779
                     /locus_tag="K0W68_001991"
     CDS             15689..17779
                     /locus_tag="K0W68_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBI3073554.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="K0W68_001992"
     CDS             complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="K0W68_001992"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBI3073555.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="K0W68_001993"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="K0W68_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBI3073556.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="K0W68_001994"
     CDS             complement(19747..20349)
                     /locus_tag="K0W68_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBI3073557.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="K0W68_001995"
     CDS             complement(20339..20506)
                     /locus_tag="K0W68_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBI3073558.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="K0W68_001996"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="K0W68_001996"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBI3073559.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="K0W68_001997"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="K0W68_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBI3073560.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="K0W68_001998"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="K0W68_001998"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBI3073561.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="K0W68_001999"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="K0W68_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBI3073562.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="K0W68_002000"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="K0W68_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBI3073563.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cobA"
                     /locus_tag="K0W68_002001"
     CDS             26714..28087
                     /gene="cobA"
                     /locus_tag="K0W68_002001"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBI3073564.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28334..28501
                     /locus_tag="K0W68_002002"
     CDS             28334..28501
                     /locus_tag="K0W68_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBI3073565.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         6995 a         7710 c         7219 g         6873 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt caccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
    28261 tcttaacgag cagtctgctt atattcatat aatcaatgaa tattaattaa tatataatac
    28321 atagggaatg taaatgaaca aatttattaa agttgcactg gtaggtgcag tactggctac
    28381 gttaactgca tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct
    28441 gctgcacccg gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta
    28501 agtctctgac agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg
    28561 agggcgtatt tgtttctatg tccttcttgc ccccgcccgt aaaaatattt tcactttaaa
    28621 ttcaatttga taactacatc cattagccac agcgcgatcc tgtgcgaaat tttgtgatct
    28681 tcctccacat tacataacat catatgttgt tatattcatc atgcattgtc atgttacctt
    28741 ttaaatgact gcaaactctc ccctacaacg tattggacaa gaaaaaggta tcgctat
//
LOCUS       DAETQN010000046        28678 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.046, whole genome shotgun sequence.
ACCESSION   DAETQN010000046 DAETQN010000000
VERSION     DAETQN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.046"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1046)
                     /locus_tag="K0W68_002003"
     CDS             complement(<1..1046)
                     /locus_tag="K0W68_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBI3073566.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSILPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..2094
                     /gene="pinE"
                     /locus_tag="K0W68_002004"
                     /pseudo
     CDS             1537..2094
                     /gene="pinE"
                     /locus_tag="K0W68_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2433..3281)
                     /locus_tag="K0W68_002005"
     CDS             complement(2433..3281)
                     /locus_tag="K0W68_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBI3073567.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3490..4125
                     /gene="yfhb"
                     /locus_tag="K0W68_002006"
     CDS             3490..4125
                     /gene="yfhb"
                     /locus_tag="K0W68_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBI3073568.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4150..4686
                     /gene="tadA"
                     /locus_tag="K0W68_002007"
     CDS             4150..4686
                     /gene="tadA"
                     /locus_tag="K0W68_002007"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBI3073569.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPSTD"
     gene            complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="K0W68_002008"
     CDS             complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="K0W68_002008"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBI3073570.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6497..10384
                     /gene="purL"
                     /locus_tag="K0W68_002009"
                     /gene_synonym="purI"
     CDS             6497..10384
                     /gene="purL"
                     /locus_tag="K0W68_002009"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBI3073571.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10942..12369
                     /gene="qseE"
                     /locus_tag="K0W68_002010"
     CDS             10942..12369
                     /gene="qseE"
                     /locus_tag="K0W68_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBI3073572.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12534..13247
                     /gene="qseG"
                     /locus_tag="K0W68_002011"
     CDS             12534..13247
                     /gene="qseG"
                     /locus_tag="K0W68_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBI3073573.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13237..14571
                     /gene="glrR"
                     /locus_tag="K0W68_002012"
     CDS             13237..14571
                     /gene="glrR"
                     /locus_tag="K0W68_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBI3073574.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14632..14970
                     /gene="glnB"
                     /locus_tag="K0W68_002013"
     CDS             14632..14970
                     /gene="glnB"
                     /locus_tag="K0W68_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBI3073575.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="K0W68_002014"
     CDS             complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="K0W68_002014"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBI3073576.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16533..17786
                     /gene="glyA"
                     /locus_tag="K0W68_002015"
     CDS             16533..17786
                     /gene="glyA"
                     /locus_tag="K0W68_002015"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBI3073577.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(17984..19177)
                     /locus_tag="K0W68_002016"
                     /pseudo
     CDS             complement(17984..19177)
                     /locus_tag="K0W68_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19295..22572
                     /locus_tag="K0W68_002017"
                     /pseudo
     CDS             19295..22572
                     /locus_tag="K0W68_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22669..23652
                     /locus_tag="K0W68_002018"
     CDS             22669..23652
                     /locus_tag="K0W68_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBI3073578.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23675..25186
                     /locus_tag="K0W68_002019"
     CDS             23675..25186
                     /locus_tag="K0W68_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBI3073579.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25211..26209
                     /locus_tag="K0W68_002020"
     CDS             25211..26209
                     /locus_tag="K0W68_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3073580.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26242..27336
                     /locus_tag="K0W68_002021"
     CDS             26242..27336
                     /locus_tag="K0W68_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBI3073581.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27348..28220
                     /locus_tag="K0W68_002022"
     CDS             27348..28220
                     /locus_tag="K0W68_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBI3073582.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28268..>28678)
                     /locus_tag="K0W68_002023"
     CDS             complement(28268..>28678)
                     /locus_tag="K0W68_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBI3073583.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6818 a         7093 c         7790 g         6977 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg caagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtatggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtga ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctactttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt tgaactgtgc aggatgcgag
     1621 ctgatttttg aagacaagat aagtggcaca aagtccgaaa ggccggggct gaaaaaactg
     1681 ctcaggacat tatcggcagg cgacacactg gttgtctgga agctggatcg gctggggcgc
     1741 agtatgcggc atcttgtcgt gctggtggag gagttgcgcg aacgaggcat caactttcgt
     1801 agtctgactg attcaattga taccagcaca ccaatgggac gctttttctt ccatgtgatg
     1861 ggtgccctgg ctgaaatgga acgtgaactg attgttgaac gaacaaaagc aggactggaa
     1921 gctgctcgcg catagggacg aattggcggt cgccgtccca aactttcacc agaataatgg
     1981 gcgcaggccg ggcgactaat tgcatcagga gtttctcgcc agaaggtggc gatcatctat
     2041 gatgttggcg aatcgacttt tttgtataag aggtttccag ccagggttat ataaaaccgc
     2101 agacacggcg tatacaagaa cgtgctacgg ttgactgaaa agttaatgtg gtatgtcaca
     2161 ggccttttgc gccgtaaagc cccgtccttt agggcgcgga ggacgttaaa gaaagtggtg
     2221 ttctttgtgt tgctgaactt atgcaaaatt tttttgcacc ataaatccaa caacacattc
     2281 aataacacat tgctgtatca ctatgaaaat aaatgtgttt ttatttgatt tcacacatat
     2341 cacaattgat gcatttttta gtgattaaca acgccataag gaaactctca aaacaacaca
     2401 aaccgggcgg gcattgtacg cgcattcttt actcagacca gttttttcac cagcgcttca
     2461 ctatgacgaa tacgttctgg tgccagttcc agatcctgct gaatcagcgc aatgaacagc
     2521 aaatccgtta acattccctg agcgtgacaa gcagagattg aagcactgtt tgtcgcttgt
     2581 tcttcggcaa tggtatacag gcaatgagaa gcacgctgtt gcagggcatt cggagtaaag
     2641 ccggtaatcg ccagcacttt tccgcccact cgcagcatct catctgccgc caggtttaac
     2701 tcgcgtcgta caccggtgta ggaaatggct aataacagat cgtcagggga cgacgcctgt
     2761 actgttgcga gtagcgcatg catatcgcgc actgcggcag cattgaagcc aatcttcatc
     2821 agcttccagg caaagttttg cgccaccaga cccgaagcgc caataccggt cagaattatc
     2881 cgccgcgcag agcgcaacat tgttacgcat tcatgcagtt tctcttcact attaacgttt
     2941 agcgttgcgt acatagcggc agtattttct ttaatcagtt tttcgccgac cagccgtaac
     3001 ggatcatcac cgcggatttg gttatgaatg ggcacggagg gcgattccgg ctggcttgcc
     3061 agcgcttcac tcaacgccaa cttaagcgcc ggaaaacctt tatagccgag tttttgcgcg
     3121 aacttcacga cgctggactg actgactccg gcttcgttgg ccagttgctg agagcttaaa
     3181 tggcgcgccg tatcaggttg tagcagcaga taatccgcca gttttttatc actctgggca
     3241 agcccctggt aacgctgacg gatacgaagt aagccgttca tatctttcct cgcgatgaaa
     3301 tagtggcgac aacaggcaat atgagacaca cgcgagtgaa ttttatattc cattgccgct
     3361 aattgccatg aattaaaaat tccagttagc catgatgcgg tcgattgttg ccggaaaagt
     3421 gatgatctat ctgttaggct atgtagtagc gaaaattaat caggatattt cagtccagag
     3481 gagtatggtt tggcaactca cgagcgtcgt gtggtgtttt ttgacttaga tggaacattg
     3541 catcagcagg atatgttcgg cagttttctg cgctatttac tgcgtcgcca accgctgaat
     3601 gcgttacttg tcctgccgtt gttaccgatt atagccattg cgttattgat aaaaggtcgt
     3661 gcggcacgct ggccgatgag tctgcttctg tgggggtgca cttttggtca cagcgaagca
     3721 cgtttacaga cgttgcaggc cgatttcgtg cgctggtttc gcgacaatgt taccgccttt
     3781 ccgctggttc aggagcgatt aaccacctac ctgttaagtt ccgatgctga tatctggttg
     3841 attaccggct ctccgcagcc gctggttgaa gcggtttatt tcgatacgcc ctggctgccg
     3901 cgggttaatc ttatcgccag ccaaattcag cgtggctatg gtggttgggt attgacgatg
     3961 cgttgtctgg gacatgaaaa ggtcgcacaa ctggagcgca aaatcggcac tccgctgcgg
     4021 ctgtacagtg gctatagcga cagtaatcag gacaatccgc tgctttattt ctgtcagcat
     4081 cgttggcgag taaccccgcg cggtgaactc cagcaactgg aatagagtaa agcatagcgt
     4141 ccgtgtataa tgcgccgcgc ttttataacc ggagttttct ttttgtctga agtcgaattt
     4201 agccacgaat actggatgcg tcacgcgctg acgctggcga aacgtgcctg ggatgagcgg
     4261 gaagtgccgg tcggcgcggt attagtgcat aacaatcggg taatcggcga aggctggaac
     4321 cgcccgattg gtcgccatga tcccaccgca catgcagaaa tcatggccct gcggcagggt
     4381 ggtctggtga tgcaaaatta tcgtctgatc gacgccacgt tgtatgtcac gcttgaacca
     4441 tgtgtaatgt gtgccggagc gatgatccac agtcgcattg gtcgcgtggt ctttggtgcg
     4501 cgtgacgcga aaactggcgc tgcgggatct ttaatggatg tgctgcatca tccgggtatg
     4561 aatcaccgag tggaaattac ggaaggaata ctggcggatg agtgcgcggc gttgctcagt
     4621 gacttctttc gcatgcgccg ccaggaaatt aaagcgcaga aaaaagcgca accctcgacg
     4681 gattaatttt gtttctcttc actccctttc ctggaaaaca gcagagaggg agaatgggtc
     4741 aaatagtttg atgacgactg ggaaagaaac gagagaaaag gaaatttctc tttgcccaac
     4801 tccgtaggcg ataccgccgg ataatcctgc gccagttgca tcgccgcttc tgtagcctgc
     4861 ttctctttct cttgcagata acccaccagg ctaatctgat acttacgaat attttcgaca
     4921 taagcgtagg cttcatgtcc acgagcgtag ccgtaagtca gcttgctgta atagggtttc
     4981 tggctaagta aaggcagacg ctgttttacg tcagcccaac tgtcaggatt ccctttggtt
     5041 tttgccgtca gggcgcgggc atccagcata tgcgcatagc ccatattgta cgcagcgagg
     5101 gcaaaccaga tccgctcgtt ctccggcaca ctttccggca ctttactcat catatcctgc
     5161 aaataacgca cgccaccgct gatgctctgt tcggcatcgg tacgatccgt aatgccgagg
     5221 ctttgcgcgg tatttttggt taacatcatc atgccgcgca cacccgtcgg tgaagtggcc
     5281 tgtgcatccc agtgcgattc ctgataagca atagcggcca gcaaacgcca gtcaatttct
     5341 tcggcgtatt tctcaaacag gggctttaac tgcggcagta ccgcatcgac ggcgcgtaaa
     5401 aatgtgcgcg tatcgacgta atcaaaatca tcgccatgcc ccaggtattt ctcttcaatg
     5461 cgtgccagcg taccgtcttc attcatttcg ttgaagaagt cgagcagggc ggcggaaagg
     5521 gtattatcgc catctaacgg gctaaaccaa gtcaccggtt gttcatcggt gatatcgagc
     5581 gctacggcga gctccgggtg aacgcgctga aacaggctga tggcgacaga atcagcaatg
     5641 gtgtaatcga gttttccttc gatgacatct tccattaatt ccgcagagcc ttttttgtcg
     5701 tctaccttcc agcttaattc cgggaatttt gtgtctttca gggtttggag atcgttaacc
     5761 accacgtgac ccggtgcaac ggtgagttgc tccgccgtca ggttgcccag tgtgcgcggg
     5821 cgatactgac tcactttata aaccagttgt tgtgacacgg aataataggt agggccaggc
     5881 tgataatttt ttacccgctc actgttatag accagtcctg ccgccagcag gtcggcgtta
     5941 ccattatcga ggtcgtcaaa cagctggctg atattctgcc gcacggtcac tttcagtttt
     6001 acaccgaggt aatcggcaaa ctgtttcgcc agttcgtaat ccaggccaaa aggtttcccg
     6061 ttgatttcgt tataagtcag gggagtatga atggtgctca cacgcaactc tccccgcgct
     6121 tgaatggcgg cgatacggtt gtcggcttta ccaaaccagg gaatggatgg ccagagagcg
     6181 accgcgagca gcagtgccag aatgccgatg aacagataat taatctttaa ttttttcaat
     6241 tagttaattc tctgtgtcgt gcgcgtccca gcttgaaaaa acgtaataat agtgaaaggt
     6301 ttactcataa atgagcggca ttttgcgtaa acctgcgcca gatggcaact tattacagcc
     6361 attggcggca cgcgttgcta attcacgatg gtgattttat ttccacgcaa acggtttcgt
     6421 cagcgcatca gattctttat aatgacgccc gtttcccccc cttgggtaca ccgaaagctt
     6481 agaagacgag agacttatga tggaaattct gcgtggttcg cctgcactgt cggcattccg
     6541 aatcaacaaa ctgctggcac gttttcaggc tgccaggctc ccggttcaca atatttacgc
     6601 cgagtatgtc cattttgctg acctcaatgc gccgttaaac gatgatgagc acgcacaact
     6661 tgaacgcctg ctgaaatatg gcccggcact cgccagccac gccccgcaag gcaaactcct
     6721 gctggtgacc ccgcgtcctg gcaccatctc tccctggtct tcgaaagcga ccgatattgc
     6781 ccataactgc gggctacaac aggtaaaccg ccttgagcgc ggcgttgctt actatataga
     6841 agccggtacg ctgaccaatg aacaatggca gcaggttacc gctgaactgc acgaccgcat
     6901 gatggaaacg gtcttttttg ctttagatga tgcagagcag ttgtttgccc accatcaacc
     6961 gactccggtt accagcgttg atttgctggg gcagggccgt caggcgctga tcgacgctaa
     7021 cctgcgtctt ggcctggctc tggcggaaga tgaaattgac tatctgcagg atgctttcac
     7081 aaagcttggt cgtaacccga acgacatcga gctgtatatg ttcgctcagg cgaactctga
     7141 gcactgccgc cacaaaattt ttaatgccga ctggattatc gacggtgaac agcagccgaa
     7201 atcgctgttc aagatgatca aaaatacttt cgaaaccacg ccagatcacg ttctctctgc
     7261 ttataaagat aacgccgccg taatggaagg ttctgaagtg ggccgctact ttgctgacca
     7321 cgaaacgggc cgctacgatt tccatcagga accggcgcat attctgatga aagtcgaaac
     7381 tcacaaccac ccgacggcga tttctccgtg gccgggcgcg gcgacgggtt ccggcggcga
     7441 aattcgtgat gaaggcgcca ccggacgcgg tgcgaagccg aaggctggtc tggttggttt
     7501 ctccgtttcc aacctgcgaa ttcctggctt cgaacagccg tgggaagaag atttcggtaa
     7561 gcctgagcgc attgtcaccg cgctggacat catgaccgaa ggcccgctgg gcggcgcggc
     7621 gtttaacaac gaatttggtc gtccggcact gaatggctac ttccgtactt atgaagaaaa
     7681 agtgaacagc cacaacggcg aagagctgcg cggttatcac aaaccgatca tgctggcggg
     7741 cgggatcggc aacattcgcg ccgatcacgt acaaaaaggc gagatcaacg tcggtgcgaa
     7801 gctggtcgtt ctcggcggcc cggcaatgaa catcggtctt ggcggcggag cggcgtcttc
     7861 catggcgtca ggtcagtctg atgccgacct cgactttgct tccgtacagc gcgacaaccc
     7921 ggagatggag cgtcgctgcc aggaagtgat cgaccgttgc tggcagcttg gtgatgccaa
     7981 cccaatcctg tttatccacg acgtcggcgc tggcggtctt tctaacgcga tgccggaact
     8041 ggtgagcgac ggcgggcgcg gcggtaaatt tgaactgcgc gatattctaa gcgacgaacc
     8101 gggcatgagc ccgctggaaa tctggtgtaa cgaatcccag gaacgctacg tgctggcggt
     8161 tgctgccgat caattaccgc tgtttgacga actgtgtaag cgtgagcgcg caccctacgc
     8221 ggtgattggt gaagcgaccg aagaactgca tctttctctg cacgatcgtc attttgataa
     8281 tcagccgatc gatctgccgc tggacgtcct gcttggtaaa acgccgaaga tgacccgcga
     8341 tgtacaaacg ctgaaagcga agggcgatgc gctggtccgt gaagggatca ccattgcaga
     8401 cgcggtgaaa cgcgtactgc atctgccgac cgtggcagag aaaaccttcc tggtgaccat
     8461 cggcgaccgc agcgtaaccg gcatggtagc gcgcgatcag atggtggggc cgtggcaggt
     8521 gccggtcgct aactgcgcgg tcactaccgc cagcctcgac agctactacg gtgaagcgat
     8581 ggcgattggc gagcgtgcgc cggttgcact gctggatttc gccgcctctg cccgtctggc
     8641 ggtcggtgaa gcgttaacca atattgccgc aacacagatt ggcgatatca aacgcattaa
     8701 actttccgcc aactggatgg cggcggcagg ccaccctggt gaagatgcgg gcctgtatga
     8761 agccgttaaa gccgtgggcg aagagctttg tccggcgctg ggcctgacga tcccggtggg
     8821 taaagactct atgtcgatga aaacccgctg gcaggaaggt aacgaagagc gcgaaatgac
     8881 gtcgccgctg tcgctggtga tttctgcatt tgcccgcgtg gaagatgtgc gtcacaccat
     8941 cacgccacag ctttctaccg aagataacac actgctgctg attgatttgg gcaaaggcaa
     9001 taacgcgctg ggcgcaacgg cgctggcgca ggtttatcgt cagcttggcg acaaaccggc
     9061 agatgtacgc gacgtcgcgc aactgaaagg cttctatgac gcgattcagg cgctggttgc
     9121 acagcgtaag ctgctcgcat accacgaccg ctctgatggc ggcctgctgg taacgctggc
     9181 ggaaatggcc tttgctggtc attgcggcat taacgcggat attgcctctc tgggcgacga
     9241 tcgcctggcg gcgttgttta acgaagaact gggtgcggtg attcaggttc gtgccgctga
     9301 ccgtgaagcg gtcgagtccg tactggcaca gcatgggctt gctgattgtg tccattatgt
     9361 agggcaggcg gtttccggtg accgttttgt gattaccgcc aacgggcaga ctgtattcag
     9421 cgaaagccgc accacgttgc gtgtctggtg ggcagaaact acctggcaga tgcagcgcct
     9481 gcgtgacaac ccggagtgtg ctgaccagga gcaccaggcg aaatcaaacg acgccgatcc
     9541 gggcctgaat gtgaaactgt cgttcgatat caacgaagat gtggcagcac cgtatattgc
     9601 cactggcgca cgtccgaaag ttgctgtgct gcgtgagcag ggcgtgaact cgcatgttga
     9661 aatggcggca gctttccacc gtgcaggctt tgatgctatc gacgtgcata tgagtgacct
     9721 gctgaccgga cgcacgggcc tggaagattt ccacgccctg gtcgcgtgcg gtggtttctc
     9781 ctacggtgat gtgctgggtg ccggtgaagg ttgggcgaag tcaatcctgt tcaatgaccg
     9841 tgtacgcgat gagtttgcaa ccttcttcca ccgtccgcaa acgctggcgc tgggggtatg
     9901 taacggttgc cagatgatgt ctaacctgcg tgaactgatc ccggggagcg agttgtggcc
     9961 acgttttgtg cgtaacacct ccgatcgctt tgaagcgcgt ttcagcctgg ttgaagtaac
    10021 ccaaagcccg tctctgctgt tgcaggggat ggtgggctcg caaatgccga ttgctgtctc
    10081 tcatggtgaa gggcgcgtgg aagtgcgtga tgcggcgcat ctggcggcac tggaaagcaa
    10141 agggctggtg gcactgcgct atgtcgataa cttcggcaaa gtcactgaaa cctacccggc
    10201 taacccgaac ggttcaccga acggtattac ggcggttacg actgaaagtg gtcgagtcac
    10261 cattatgatg ccgcacccgg aacgtgtttt ccgtactgtc agcaactcct ggcatccgga
    10321 aaactggggc gaggatggcc catggatgcg cattttccgc aatgcgcgta agcagttggg
    10381 gtaagtcatc agttcattgg ttttgcagcc cggatgcggc attaagcacc aaccactatc
    10441 ataagctccc aactccggga gcttttttgt gtctgtaaat cacgacaatg ggtggtttac
    10501 cgtgtcgctt tctggcgaca cttaaatgat tgattttatt gcaatctaat aagtttatct
    10561 taagttgtct ctttttagcg acacagtggc tgatttacat tcaaatagcg aagaagccat
    10621 aaatccctca aagcaacgta taatcagtac gttaccaaac tattttcttt attggcacag
    10681 ttactgcata atagtaagca gtggctcatt caccgactta tgtcagcccc ttcgggacgt
    10741 gctacataaa attcgaatga cgcacaacaa ggtgcctgcc gtccaacttc tgatatcagc
    10801 gtagctatat caaccatcgg gcgaaacgtc gagttaggca ccgccttatt ccataacaaa
    10861 gccgggtaat tcccggcttt gttgtatctg aacttcccct cggttagcat caggctattc
    10921 gcgtctgacg agagtaacac cttgaaacgc tggcccgttt ttccccgctc attacgacaa
    10981 ctggtaatgc tggcattttt gctaattcta ctacccctgt tggtgctggc atggcaagcc
    11041 tggcaaagcc tgaatgcgct tagcgatcag gcggcgctgg ttaaccgcac tacgcttatt
    11101 gatgcccggc gtagtgaagc gatgaccaac gcggcgctgg agatggagcg tagctaccgt
    11161 cagtattgcg tgctggacga ccctacgctt gcgaaggttt atcaaagcca gcgcaagcgt
    11221 tacagcgaaa tgctcgatgc ccacgcaggc gtactgccag acgataaact ttaccaggca
    11281 ttgcgtcagg atttgaacaa tctggcccaa cttcagtgta acaacagtgg tcccgatgct
    11341 gccgccgccg cgcgtcttga agcctttgcc agtgccaata ccgaaatggt gcaggccacg
    11401 cgcacagtgg tgttctctcg tgggcagcaa cttcagcgtg aaatcgccga acgtgggcaa
    11461 tattttggtt ggcaatcgct ggtgctattt ctggtgagtc tggtgatggt gctacttttc
    11521 acgcggatga ttatcgggcc ggtgaaaaat atcgagcgca tgatcaaccg tctgggggaa
    11581 gggcgttctc tgggcaatag cgtttcgttc agtggaccga gcgagttacg ctcggttggg
    11641 caacgtattc tttggttaag tgagcgcctg tcatggctgg aatcccaacg ccatcaattt
    11701 ttaagacatt tatctcatga attaaaaacg ccactggcga gtatgcgcga gggcactgaa
    11761 ttactggctg accaggttgt cgggccgctt acgccagagc aaaaagaggt ggtgagcatt
    11821 cttgatagca gcagccgcaa tttgcaaaaa ctgatcgaac aactgcttga ttacaaccgt
    11881 aaacaggcgg acagtgcggt ggaactggag aatgttgaat tagcaccgct ggtggagaca
    11941 gtggtttctg ctcatagcct gcccgcacgg gctaaaatga tgcataccga cgtcgatctc
    12001 aaagcaacag cttgcctggc ggagccaatg ctgctgatga gcgtactgga taatctttac
    12061 tccaatgcgg tgcactacgg ggctgaatcc ggtaacattt gccttcgcag cagttcacat
    12121 ggtgcgcggg tttatattga tgtcatcaat acaggcacgc ccattccgca agaggaacgc
    12181 gccatgatct tcgaaccctt ttttcaggga agccaccagc gaaaaggggc ggtgaagggc
    12241 agcggtctgg gattaagcat tgccagggat tgtattcgcc gtatgcaagg ggaactgtat
    12301 ctggtcgacg agagcgggca agacgtttgt ttccgcattg aattaccgtc gtcgaaaaac
    12361 acgaaataat gctaacagcc tatcctatca ggctatttta tttgtcagtt cggccccggg
    12421 cagtgctcga aatcttcacg tacgatatgt acgctccggt ttctccgcgc tgtccatgtc
    12481 cagactgtct gcaacaatta cgcccactgg gataggcttt aagtctggtg aatatgcgac
    12541 acatttttca acgattattg ccccgacggc tgtggctggc agggctgcca tgtctggcat
    12601 tgctgggttg cgtgcagaat cacaataagc cagccattga tacgccagca gaagaaaaaa
    12661 ttccggttta tcaactggct gactatcttt ctactgaatg tagcgacatc tgggcgctgc
    12721 aaggtaaatc aaccgaaacc aatccgcttt actggctgcg ggcgatggat tgtgctgatc
    12781 gtttaatgcc tgcgcagtcg cgtcagcaag ctcgccaata tgacgacggc agctggcaaa
    12841 atacctttaa acaagggatt ttgctcgccg acgccaaaat tacgccgtat gagcgccgcc
    12901 agctggtcgc gcgtattgaa gcgttaagta ccgagatccc ggcacaggtt cgcccactgt
    12961 atcaactctg gcgcgacggt caggcgctgc aactgcaact ggcggaagag cgccaacgtt
    13021 acagtaaact ccagcagtcc agcgacagcg agctggatac attgcgccag caacatcacg
    13081 ttctacagca gcaactggaa ctcaccaccc gcaagctgga aaacctgacc gatattgaac
    13141 gccagctctc gacccgcaaa ccgactggaa atttctcgcc ggatacgccg cacgagagtg
    13201 aaaagcccgc gccatccacc catgaggtca ctcctgatga gccataaacc tgcgcattta
    13261 ttattggtcg atgacgatcc gggattgctg aaactgcttg gcctgcgcct gaccagcgaa
    13321 ggctacagtg tggtcacggc ggaaagtggc gctgaaggat tacgggtact gaatcgcgaa
    13381 aaagtagatt tagttatcag cgacctgcgg atggatgaaa tggacggtat gcagctattt
    13441 gctgaaatcc agaaagtgca gccgggaatg ccagtaatta ttcttactgc gcatggttct
    13501 atccccgatg ccgttgctgc aacacagcag ggcgttttta gtttcctcac caagcctgtc
    13561 gataaagatg cgctatatca ggcaattgac gatgcgctgg agcaatccgc gccagccacc
    13621 gatgaacgct ggcgcgaggc aattgtcacc cgcagcccgc tgatgctgcg tttgctggaa
    13681 caggcgcggc tggtggcgca atcagacgtc agcgttttga ttaacggtca gagcggcacc
    13741 gggaaagaga ttttcgccca ggctatccac aacgccagcc cgcgcaacag caaaccattt
    13801 attgctatta actgtggcgc attgcccgag caattgctgg agtcggagct gtttggtcat
    13861 gcgcgtggcg cgtttactgg cgctgtcagc aatcgcgaag gtttattcca ggcggcggaa
    13921 ggcggaacgc tatttctcga tgaaattggc gatatgcctg cgccgttaca ggtcaaactg
    13981 ctgcgcgtgt tgcaggagcg taaagtgcgc ccgctgggca gtaaccgcga tattgatatc
    14041 aatgtgcgga ttatttctgc cactcaccgt gatctgccaa aagcgatggc gcgcggggaa
    14101 ttccgcgaag atctctatta ccgcctcaac gttgtcagcc tgaaaattcc ggcactggcg
    14161 gagcgcacag aagacattcc gctactggca aatcacctgt tgcgccaggc ggcagagcga
    14221 cataaaccgt ttgtccgcgc gttctctacc gatgcgatga aacgcctgat gaccgcgagc
    14281 tggccgggta atgtgcgcca gttggtcaac gtgattgaac agtgcgtggc gctgacctca
    14341 tctccggtga ttagtgatgc gctggtggag caggcgctgg agggtgaaaa tacggcgctg
    14401 ccaacctttg ttgaggcacg taatcagttt gaactcaact atttgcgtaa gctgctgcaa
    14461 atcaccaaag gcaacgtcac ccacgcggcg agaatggcgg ggcgcaaccg gacagaattt
    14521 tataaactgc tttcccgaca cgagctggat gcaaacgatt tcaaggaatg aattggcgtt
    14581 atgtgttacg tttagcagat caaaagacag gcgacctttt caaggaatag catgaaaaag
    14641 attgatgcga ttataaaacc cttcaagctg gacgatgtcc gcgaagcact ggccgaagtc
    14701 ggtattaccg gcatgacggt gaccgaagtg aaaggctttg gtcgccagaa aggccatacc
    14761 gagctgtacc gcggcgcgga gtatatggtg gattttctgc cgaaagtgaa aattgagatt
    14821 gtcgtaccgg acgacattgt cgatacctgt gtcgatacca ttattcgcac ggcgcaaacc
    14881 ggcaaaatcg gtgacggtaa aatcttcgtc tttgacgtgg cacgggtcat tcgcatccgt
    14941 accggtgaag aggacgacgc ggcaatttaa accgcgtcaa gggttgccgg atgtttccat
    15001 ccggcaacat caaattacag caccttatgc gggccaaagc attcgtaatg aatgttttcc
    15061 tgcttcacgc ccagatccac taactgtttc gcggtaaact gcatgaagcc aaccgggccg
    15121 catagataga actgcattgt cggatcgctg aacgcacctt ccagtttgct caaatccatc
    15181 agaccttcgc tatcaaactg acctttagcg cgatcggctt cgctcggctg acgatgccag
    15241 gtgtgcgcgg taaagcgcgg cagtgactgc cccagttcct taacttcatc ggcaaaggcg
    15301 tgaacatcgc cattttctgc cgcatggaac cagttcactt gtgctgtgtg gcctgctttt
    15361 gccagcgtgt cgagcattgc cagcattggc gtttgaccaa caccggcaga gattaacgtc
    15421 actggtgtgt catctgcgac agccataaag aaatcacctg ccggagcgac cagtttcacg
    15481 acatcgccaa cattggcgtg attgtgcaac cagttggata cctgcccacc ctcttcgcgt
    15541 ttcaccgcaa tacgatagcc tttgccatcc ggtttgcgag tcaaagagta ctgacgaatt
    15601 tcctgatgcg ggaaaccttc cggcttcagc cagacgccga gatattgccc cggacggtat
    15661 tctgccactg cgccaccgtc gaccggctcc agttcgaagc tggtgataag cgcgctgcgc
    15721 ggtgttttag ccacaatgcg gaaatcgcga gtaccttccc aaccaccggc tttgctggcg
    15781 ttttcgttat agatttccgc ctcgcgattg ataaatacat tagccagtac accataggct
    15841 ttaccccacg cgtccagcac ttcctgcccc gggctgaaca tttcgtccag cgttgccaac
    15901 aggtgttcac cgacgatgtt gtactgttcc ggtttgatct ggaagctggt gtgcttctgc
    15961 gcgatttttt ctaccgctgg cagcagcgca ggcaggtttt caatattact ggcgtaggcg
    16021 gcaatagcgt taaacagggc ttcacgttga tcgccattac gctggttact catgttaaaa
    16081 atttctttga gttctgggtt atgagtaaac atacggtcgt agaaatgggc ggttaacttt
    16141 ggccccgttt ccaccagtaa agggatggtg gcttttactg tagcgatggt ttgagcgtca
    16201 agcatatggt cttccttttt ttgcatctta attgatgtat ctcaaatgca tcttataaaa
    16261 aatagccctg caatgtaaat ggttctttgg tgtttttcag aaagaatgtg atgaagtgaa
    16321 aaatttgcat cacaaacctg aaaagaaatc cgtttccggt tgcaaactct ttattctcca
    16381 aagccttgcg tagcctgaag gtaatcgttt gcgtaaattc ctttgtcaag acctgttatc
    16441 gcacaatgat tcggttatac tgttcgccgt tgtccaacag gaccgcctat aaaggccaaa
    16501 aattttattg ttagctgagt caggagatgc ggatgttaaa gcgtgaaatg aacattgccg
    16561 attatgatgc cgaactgtgg caggctatgg agcaggaaaa agtacgtcag gaagagcaca
    16621 tcgaactgat cgcctccgaa aactacacca gcccgcgcgt aatgcaggcg cagggttctc
    16681 agctgaccaa caaatatgct gaaggttatc cgggcaaacg ctactacggc ggttgcgagt
    16741 atgttgatat cgttgaacaa ctggcgatcg atcgtgcgaa agaactgttc ggcgctgact
    16801 acgctaacgt ccagccgcac tccggctccc aggctaactt tgcggtctac accgcgctgc
    16861 tggaaccagg tgataccgtt ctgggtatga acctggcgca tggcggtcac ctgactcacg
    16921 gttctccggt taacttctcc ggtaaactgt acaacatcgt tccttacggt atcgatgcta
    16981 ccggtcatat cgactacgcc gatctggaaa aacaagccaa agaacacaag ccgaaaatga
    17041 ttatcggtgg tttctctgca tattccggcg tggttgactg ggcgaaaatg cgtgaaatcg
    17101 ctgacagcat cggtgcttac ctgttcgttg atatggcgca cgttgcgggc ctggttgctg
    17161 ctggcgtcta cccgaacccg gttcctcatg ctcacgttgt tactaccacc actcacaaaa
    17221 ccctggcggg tccgcgcggc ggcctgatcc tggcgaaagg tggtagcgaa gagctgtaca
    17281 aaaaactgaa ctctgccgtt ttccctggtg gtcagggcgg tccgttgatg cacgtaatcg
    17341 ccggtaaagc ggttgctctg aaagaagcga tggagcctga gttcaaaact taccagcagc
    17401 aggtcgcgaa aaacgctaaa gcgatggtag aagtgttcct cgagcgcggc tacaaagtgg
    17461 tttccggcgg cactgataac cacctgttcc tggttgatct ggttgataaa aacctgaccg
    17521 gtaaagaagc agacgccgct ctgggccgtg ctaacatcac cgtcaacaaa aacagcgtac
    17581 cgaacgatcc gaagagcccg tttgtgacct ccggtattcg cgtgggtact ccggcgatta
    17641 cgcgtcgcgg cttcaaagaa gcagaagcga aagaactggc tggctggatg tgtgacgtgc
    17701 tggacagcat caatgatgaa gccgttatcg agcgcatcaa aggtaaagtt ctcgacatct
    17761 gcgcacgttt cccggtttac gcataagcga aacggtgatt tgctgtcaat gtgctcgttg
    17821 ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aaactttgca aattcaatat
    17881 attgcaatat ccgtgtaggc ctgataagcg tagcgcatca ggcaatctta ggtatgtgat
    17941 catcaaggct tccttcggga agcctttcta cgttattgcg ccatcaaatc tgtcgtaact
    18001 gcgcctcaac atacaaatag ccaatcccca acacctgttg tgcgcggctt aattgcccaa
    18061 agccaatttg cgttgctttc acgctcggcc cttcgtcgcg gtcgaacggg ttaaatcccg
    18121 tctggcgaat aatggtatta agccagtgct caccaaaggc acaactgcga ccgtacaacc
    18181 aaatctgatt aatattgagg atgttgagga agttatacag actcaaacca atggcattgg
    18241 cactgcgatc aacccaactg gtgatccacg gttctccact ctaccaggca gcgattaact
    18301 gcgctgtagt cagtttttcc ggatcaagct gagtattaac cggttgtgat tttagccata
    18361 cccgcgcctg tttttttaat gcacttaacg aggcgacggt ttccaggcag ccgtaacgtc
    18421 cacagtcgca gacgacgcca tccggattaa cgatggtgtg actaatctgt ccgctaccat
    18481 acaaactgcc gcgataaatt tgctcgttaa tcacgaacga cgagccaatg ccgtaatcaa
    18541 cgttgatcac gcagaaatcc cgtttctgcg aattattttg ccatttctcc gccagcgcca
    18601 gcatcacgca gtcattatcg accatcaccc gaatgccgag cttctcttcc agcagatact
    18661 tcacctcaac cggcgttgtc cacggcgctt gcggcatggt ttgcgacacg ccggtcaccg
    18721 gatcaacctg accgtggatt gccagcgcca gattgatggt gcggtccggc cacaatttac
    18781 ggtggcgatg ccagcatttt tcgatttcgg acagcagcgc ctgcggcgtc ggtgcatcaa
    18841 tctgtaaata ctcaaattca cctttcggac ttaaacaagc attagcaacc tgacactcaa
    18901 tactggtggg cgtcacgttc aggcacagcg tccagtcacc ttccggcgca atcagccatg
    18961 taccgctact atgcccgcgc gtctggcttt catcgtcgat attcaccacc cgtttttcgc
    19021 tttccagttc ctgcaaaata ttactgactg ccggaatcga aatttgcgcc agccgggcca
    19081 gcgttgattt attggcgcgc ttttgccggt acagcagttc cagaatcacg catttgttat
    19141 ggtggcgaat ctgttgattg ttaatgcagg ctctcatctt cactaaacct tgtttagtta
    19201 attgcgtgat tattccgcga aaacattgcc agattgttca atacactgcc acaaatcttt
    19261 taattcagta tgtcttgtta atattgaggg caccatgact ccagtaaaag tgtggcaaga
    19321 gcgcgttgag atcccgacct atgaaaccgg gccgcaggat atacatccca tgttcctgga
    19381 aaatcgcgtt tatcagggat cgtccggcgc ggtttatccc tacggcgtga ccgatacgct
    19441 gagcgagcag aaaaccctga aatcctggca ggcggtgtgg ctggaaaacg actacatcaa
    19501 agtgatgatc ctgccggaac tgggcggtcg ggtgcatcgc gcgtgggata aagtgaaaca
    19561 gcgcgatttt gtttatcaca atgaagtcat taaacctgcg ctggtggggc tgctggggcc
    19621 gtggatttcc ggtgggattg agtttaactg gccgcaacat catcgcccga ccacctttat
    19681 gcccgttgat ttcaccctcg aagcccatga agacggtgca cagacggtgt gggtaggcga
    19741 aacggagccg atgcatggtt tacaggtgat gacaggtttc accctgcgcc ctgggcggcg
    19801 ctggaaatcg ccagccgcgt ctataacggc aacgccacgc cgcgtcattt cttgtggtgg
    19861 gccaacccgg cagtgaaagg gggtgaaggg catcagagcg ttttcccacc ggatttgact
    19921 gcggtgtttg atcacggcaa acgggccgtc tccgctttcc ccatcgctac cggcacttac
    19981 tacaaagtgg actactccgc tggagtggac atttctcgct ataaaaatgt gcccgttcca
    20041 acctcatata tggccgaaaa atcgcagtac gattttgtcg gcgcgtggtg tcacgatgaa
    20101 gatggcggtt tgctgcacgt tgccaaccac catattgcgc caggtaaaaa acagtggagt
    20161 tggggacaca gtgaatttgg ccaggcgtgg gataagagtc tgaccgacaa taacggcccg
    20221 tatatcgaac tgatgaccgg tatttttgcc gataaccagc ccgattttac ctggcttgat
    20281 gcttacgaag agaagcgttt tgagcagtat ttcctgcctt atcattctct gggcatggtg
    20341 caaaatgcct cccgcgatgc ggtgataaaa ctccagcgta gtgagcaggg gattgagtgg
    20401 gggctgtatg ccatctctcc gttgaacgga taccgcctgg cgatccacga aatcggcaaa
    20461 tgcaacgcgt tacttgatga tgccgtggca ctgatgcctg cgaccgccat ccagggcgtg
    20521 ttgcacggta tcaatcctga taggctgacc attgagcttt ccgatgccga cggcaatatt
    20581 gtactgagtt atcaggaaca tcagccgcaa gcgttgccgt taccggacgt cgccaaagcg
    20641 ccactggcag cacaagacat taccagtaca gatgaagcct ggtttatcgg tcagcatctg
    20701 gagcaatatc atcacgccag ccgttcaccg ttcgattact acctgcgcgg cgtggcgctg
    20761 gacccgctgg attatcgctg taacctggcg ctggcgatgc tggaatataa ccgcgcagat
    20821 ttcccgcaag cggtggcgta tgccagtcag gcgctgaaac gcgcacatgc gctgaacaaa
    20881 aacccgcagt gtggacaagc aagtttgatt cgcgccagtg cttacgaacg tcagggacaa
    20941 tatcaacaag ccgaagagga tttctggcgt gcggtctgga gcggcaacag caaagccggt
    21001 ggctattatg gcctggcacg actggcggcg cgtaatggta acttcgacgc tggtctggat
    21061 ttttgccaac aaagtcttcg cgcctgccca accaatcagg aagtgctttg cctgcataat
    21121 ctgctgctgg tgttaagtgg tcgtcaggac aacgcgcgtt tgcagcgcgc gaaactgctg
    21181 cgtgattatc cgctgaacgc cattctgtgg tggctgaact ggttcgatgg tcgtagcgaa
    21241 tcagccctcg cgcagtggcg cggtctgtgt cagggacgcg acgttaacgc cctgatgacc
    21301 gccgggcaac tgattaactg gggaatgccc actctggcga cagagatgct gaacgcactg
    21361 gactgtcagc gcacgttatc gctttatcta caagccagct tgctgccgaa agccgaacgc
    21421 ggcgaactgg tcgcaaaagc cattgatgtc ttcccgcagt ttgtccgttt cccgaatacg
    21481 ctggaagaag tggcggcgct ggagagtatt gaagagtgct ggtttgcccg tcatttactg
    21541 gcctgtttct actacaacaa gcgcagctac aacaaagcca ttactttatg gcaacgttgc
    21601 gtagagatgt cgccggagtt tgccgatggc tggcgcgggt tggcgatcca tgcgtggaat
    21661 aagcaacacg attatgaact ggccgcgcgt tatcttgata atgcttatca gcttgcgccg
    21721 caggatgcac gtctgctttt cgaacgggat ttgctggata agttaagtgg agccacaccg
    21781 gagaaacgac tggcgcgtct ggaaaataat ctggaaattg cgctgaaacg cgacgacatg
    21841 accgcagaac tgctcaattt gtggcatctc acgggtcagg cagacaaagc ggcggacatt
    21901 ctcgccacgc gcaaattcca cccgtgggaa ggcggggaag ggaaggtcac cagtcagttt
    21961 atcctcaacc agttattacg cgcctggcag catcttgatg ccagacagcc gcagcaggcc
    22021 tgcgaactgc ttcatgccgc gctgcattat ccggagaatt taagcgaagg ccgtttaccg
    22081 gggcaaactg ataacgacat ctggttctgg caggcgatat gcgccaacgc gcagggcgat
    22141 gaaactgaag cgacgcgttg tttacgtctg gcggcgaccg gcgatcgcac cattaacatc
    22201 cacagttatt acaacgatca gccggttgat tatctcttct ggcaaggaat ggcgctgcga
    22261 ctgctgggcg aacaacaaat cgcacagcaa ctgtttagtg aaatgaaaca gtgggcgcaa
    22321 gagatggcga aaaccagtat tgaggcggat ttctttgctg tttcacaacc tgacctgttg
    22381 tcgctgtatg gcgatttaca acagcagcat aaagaaaaat gcctgatggt ggcgatgctg
    22441 gcgtccgcgg gactcgggga ggttgcgcaa tatgaatctg ctcgcgctga attgacggcg
    22501 attaatccgg cctggccgaa agcggcatta ttcaccaccg tgatgccttt tatttttaac
    22561 tacgttcact aaaccctatt aactgtaata tttgagcggc acgcgctgcc gctcatcaca
    22621 aaataattag aataagccgt tgttaataaa aactctacag gaggcactat gcctaaaaaa
    22681 atgagaacaa cccgtaatct attattaatg gctacgctat taggtagcgc actatttgcc
    22741 agggctgcgg ataaagaaat gaccattggt gcaatatacc ttgataccct gggatattac
    22801 gctggagtgc gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca ggtacagtta
    22861 attgaaacta acgcccaggg tgatatttcg aaagaaagta cctttgttga taccctcgtg
    22921 gcgcgtaatg tcgatgccat tattttatcg gcagtgtctg aaaatggcag tagccgtacc
    22981 gttcgtcgcg ccagtgaagc gggtattccg gtgatttgct acaacacctg tattaatcaa
    23041 aagggtgtcg ataaatatgt ctcggcgtat ctggtcggcg atccactgga atttggtaaa
    23101 aaactgggta acgctgccgc cgattatttt attgccaata aaattgacca gccgaaaatt
    23161 gccgtcatca attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa
    23221 gtattaaaat cccgcgttcc cggcgcgcaa attgtcgcta atcaggaagg gactgtttta
    23281 aataaagcga tttccgttgg tgaaaaactg attatctcca cgccggatct caacgccatt
    23341 atgggggagt caggcggtgc gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag
    23401 gccggaaaaa ttgctgtttt cggttcggat atgacaaccg aaattgctca ggagctggaa
    23461 aacaatcagg tgctgaaagc ggtagtggat atttccggta agaaaatggg caatgctgtt
    23521 ttcgcgcaaa cattgaaggt tatcaataaa caagccgacg gtgaaaaagt gattcaggtg
    23581 ccaatcgatc tctataccaa aacggaagat ggtaaacagt ggctggcaac gcacgttgat
    23641 ggtctgccct aatcgctgat tcgcagaggt ccctatgttc acggcaacag aggcagtccc
    23701 ggtagcaaaa gtggtggcag gaaataagcg ttaccccggc gtcgttgcgc tggataacgt
    23761 taacttcacg ctcaataaag gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa
    23821 atcgactctc attcgaatgc ttaccggcag cgaacgcccg gatagcggtg atatctggat
    23881 tggcgagacg cgactggaag gtgacgaagc tacgttgact cgccgtgccg ctgaactggg
    23941 ggttcgtgcg gtttatcagg aattaagtct ggtggaaggg ctgacggtgg cagaaaacct
    24001 ctgccttggt caatggcctc gccgcaacgg catgattgat tacctgcaaa tggcgcagga
    24061 tgcccaacgt tgcttacagg cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac
    24121 gctaagcccg gcgcaaaagc aactggtgga aattgcacgg gtgatgaagg gcgagccgcg
    24181 cgtggtcatt cttgatgaac ccaccagctc gcttgccagt gcagaagttg aactggtgat
    24241 cagtgcggtg aaaaagatgt cggcactggg cgtggcggtg atttatgtca gccaccggat
    24301 ggaagaaatc cgccgcattg cctcctgtgc caccgttatg cgcgatggtc aggtggcggg
    24361 cgatgtgatg ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc tagggcgtga
    24421 tcacgttgat attgcgccgg ttgcccctca ggaaattgtg gatcaggccg tactggaagt
    24481 ccgtgcgtta cgccataagc ccaagctgga ggatatcagc tttacgttac gtcgcggcga
    24541 agtgcttggc attgctggcc tgctgggggc agggcgcagt gaattgctga aggcgattgt
    24601 tgggctggag acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa tcacgcgccc
    24661 cgattacggc gacatgctga aacgcggcat tggctatacg ccagaaaacc gcaaagaagc
    24721 ggggatcatt ccctggttgg gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat
    24781 cagcgccaac ggagtgctgc aatggtccac catccgccgc ctgaccgaag aggtgatgca
    24841 gcggatgacg gtcaaggccg ctagtagcga aacgcccatc ggcacgcttt ctggtggcaa
    24901 tcagcaaaaa gtggtgatcg gtcgttgggt ctatgccgcc agccagattt tgttgctcga
    24961 cgagccaacg cgcggcgtcg atatcgaagc caaacagcag atttaccgta ttgtccgtga
    25021 gctggctgcc gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg agttgccgct
    25081 ggtgtgtgac cgcatcctgt tattacagca cggcacgttc tcgcaggagt ttcactcgcc
    25141 ggtcaatgtg gatgagctga tgtccgccat tctgtctgtg cactgatgaa ttttaccgag
    25201 gaacctgaag atgtctgctt cgtcattacc attgccgcag ggcaagagcg tctcgctcaa
    25261 acaatttgtc agtcgccata ttaatgagat cggtttgctg gtggtgattg ccattcttta
    25321 tctggtcttc tccctgaacg cgcctggctt tatctcattg aataaccaga tgaacgtgct
    25381 gcgcgatgcc gccaccattg ggattgccgc ctgggcgatg acgctgatta ttatctccgg
    25441 tgaaattgat gtcagcgttg ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt
    25501 gctgcaattt gaagttccgt tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct
    25561 gatgggaacg ctcgccgggg tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac
    25621 actggggctg tggagcgccc tgcgcggaat ggggctgttt atgaccaacg cactgccagt
    25681 gccgattgac gaaaacgagg tgctggactg gctgggcgga caatttctcg gtgtgccagt
    25741 atccgcgctg atcatgatgg tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc
    25801 cttcgggcgc tcggtttttg ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat
    25861 taacgttcgt cgggtgcgca ttcttatctt taccctttcg ggattattag cggcggtgac
    25921 cggcattttg ctggcggcac gcctcggttc cggtaacgca ggtgccgcaa acggtctgga
    25981 gtttgacgtc atcgccgcgg tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc
    26041 cctgttcggt acattgcttg gtgtgctggt gattacgcta atcggtaacg gtctggtgct
    26101 gctcggtatt aactcctttt tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt
    26161 gctggcgaat atcttgctga cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc
    26221 cctggcgcgg gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa
    26281 acgatgctgg cagcttattt accaggaaat tcaaccgtcg atctgcggga agttgcggtg
    26341 ccgacgccgg ggattaacca ggtactgatc aaaatgaaat cctccgggat ttgcggaagc
    26401 gatgtccact atatctatca tcaacaccgt gccacggcgg cggcacccga taaaccgtta
    26461 taccagggat ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat ggggcaaggc
    26521 tgccgccatt ttaaagaggg cgaccgcgtg ctggtgtatc acatttctgg ctgtggtttt
    26581 tgcccgaact gccgccgcgg ctttcctatc tcttgtactg gcaaaggaaa agcggcttac
    26641 ggctggcagc gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa agatctgatc
    26701 ctcctgccgg atgcgctgag ctacgaagat ggtgcgttta tcagttgcgg cgttggcacg
    26761 gcctatgaag ggattttgcg cggcgaagtt tccggcagcg ataacgtgct ggtggtcggt
    26821 ctggggccgg tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc
    26881 atcggcgttg atatgctgcc ggaacgtctg gcgatggcaa aacagttagg ggtgatggat
    26941 cacggctatt tagcaaccac cgaaggtctg ccgcagatta tcgccgaact cacccacggt
    27001 ggcgcggatg ttgcgctcga ttgttctggt aatgccgcag gtcgcttgct ggcactgcaa
    27061 tccaccgctg actggggacg ggtggtttac attggtgaaa ccggaaaagt ggaattcgag
    27121 gtcagcgccg atctgatgca ccatcaacgg cggattatcg gctcctgggt gaccagtctg
    27181 ttccatatgg aaaaatgcgc ccacgatttg actgactgga agctgtggcc gcgtaacgcc
    27241 attacccatc gcttctcgct ggaacaggca ggtgatgcct atgcgctgat ggcgagcggc
    27301 aaatgcggga aagttgtgat taacttcccg gattaaggag ttagcggatg accatctata
    27361 ccttatccca cgggtcgcta aagctggacg tttctgacca gggcggcgta atcgaaggct
    27421 tctggcgcga tacgacgccg ctcttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg
    27481 cctcatgttt tccgctggtg ccatttgcaa accgggtgag cggcaaccgt tttgtctggc
    27541 aggggcgtga gtatcaactg caaccgaatg ttgagtggga tgcgcactat ctacacggcg
    27601 atggctggct gggcgagtgg caatgtgtct cgcacagcga tgatagtttg tgtctggtgt
    27661 atgaacatcg cagcggtgtc tatcactatc gggtaagtca ggcgtttcat ttaacggcgg
    27721 atacgctgac ggtgacgctc tctgtcacca atcaaggggc agagacgctg ccatttggta
    27781 ccggctggca tccttatttt ccgttgtcac cacaaacgcg gattcaggcg caggcgagcg
    27841 gttactggct ggaacgggag cagtggctgg cgggtgagtt ttgcgagcag ctaccgcagg
    27901 aactggattt taaccagctc gcgccgttgc cgcgccagtg ggtgaacaat ggttttgccg
    27961 gatggaatgg tcaggcccgc atcgagcagc cgcaggaagg ttatgccatc atcatggaaa
    28021 cgacgccacc tgcaccgtgt tatttcatct ttgtttccga ccctgcgttt gataaaggat
    28081 atgcgtttga tttcttctgt ctggaaccga tgagccatgc gccggatgat catcatcgcc
    28141 cggaaggggg tgatctcatt gcgctagcac caggggaatc aacaacttca gagatgtcgt
    28201 tgcgggtaga gtggctgtaa tttattaaat gccggataag gcgttcacgc cgcatccggc
    28261 atctttccta acgccgatcg agagaaattg cccccggccc ggtaattgcc aatagcaaga
    28321 acgcgccagc gatactgaca ttcttccaga aattaatcat atttggccca accgcatcgc
    28381 cggtcatatc ccagtaatgg tgaccaatca ccgccgtacc cagcgtgtag aaaataaaca
    28441 gcaccgccag cggacgggtg aaaaagccaa gcacgattaa tatcgcggcg ggcacttcca
    28501 taactaccgc aataatcgct gccagcatcg gcattggcgc gcccaacgag gccatatatt
    28561 ggaccgtacc gtcaaagccc atcattttgg gaaaaccaaa aataatgaaa attaagacca
    28621 cggcgatacg ggcaattaat aacaaaacgg ggcgggcagc tccaaaatca aaataacg
//
LOCUS       DAETQN010000047        28041 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.047, whole genome shotgun sequence.
ACCESSION   DAETQN010000047 DAETQN010000000
VERSION     DAETQN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.047"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..133)
                     /locus_tag="K0W68_002024"
     CDS             complement(<1..133)
                     /locus_tag="K0W68_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3073584.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     "
     gene            141..572
                     /gene="tnpA"
                     /locus_tag="K0W68_002025"
                     /pseudo
     CDS             141..572
                     /gene="tnpA"
                     /locus_tag="K0W68_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(703..2026)
                     /locus_tag="K0W68_002026"
                     /pseudo
     CDS             complement(703..2026)
                     /locus_tag="K0W68_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(2026..3642)
                     /locus_tag="K0W68_002027"
     CDS             complement(2026..3642)
                     /locus_tag="K0W68_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBI3073585.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3938..4654
                     /locus_tag="K0W68_002028"
     CDS             3938..4654
                     /locus_tag="K0W68_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI3073586.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4651..6312)
                     /locus_tag="K0W68_002029"
     CDS             complement(4651..6312)
                     /locus_tag="K0W68_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBI3073587.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6508..6936)
                     /gene="ibpB"
                     /locus_tag="K0W68_002030"
     CDS             complement(6508..6936)
                     /gene="ibpB"
                     /locus_tag="K0W68_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBI3073588.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(7048..7461)
                     /gene="ibpA"
                     /locus_tag="K0W68_002031"
     CDS             complement(7048..7461)
                     /gene="ibpA"
                     /locus_tag="K0W68_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBI3073589.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7455..7527)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7767..8099
                     /locus_tag="K0W68_002032"
     CDS             7767..8099
                     /locus_tag="K0W68_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBI3073590.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(8101..9315)
                     /locus_tag="K0W68_002033"
     CDS             complement(8101..9315)
                     /locus_tag="K0W68_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HBI3073591.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9416..10480
                     /gene="cbrA"
                     /locus_tag="K0W68_002034"
     CDS             9416..10480
                     /gene="cbrA"
                     /locus_tag="K0W68_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBI3073592.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10477..11814)
                     /locus_tag="K0W68_002035"
     CDS             complement(10477..11814)
                     /locus_tag="K0W68_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3073593.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11889..13037)
                     /gene="dgoD"
                     /locus_tag="K0W68_002036"
                     /pseudo
     CDS             complement(11889..13037)
                     /gene="dgoD"
                     /locus_tag="K0W68_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(13034..13651)
                     /gene="dgoA"
                     /locus_tag="K0W68_002037"
     CDS             complement(13034..13651)
                     /gene="dgoA"
                     /locus_tag="K0W68_002037"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBI3073594.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13635..14513)
                     /gene="dgoK"
                     /locus_tag="K0W68_002038"
     CDS             complement(13635..14513)
                     /gene="dgoK"
                     /locus_tag="K0W68_002038"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBI3073595.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14510..15199)
                     /gene="dgoR"
                     /locus_tag="K0W68_002039"
     CDS             complement(14510..15199)
                     /gene="dgoR"
                     /locus_tag="K0W68_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBI3073596.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15168..15800
                     /locus_tag="K0W68_002040"
                     /pseudo
     CDS             15168..15800
                     /locus_tag="K0W68_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15846..16658)
                     /gene="yidA"
                     /locus_tag="K0W68_002041"
     CDS             complement(15846..16658)
                     /gene="yidA"
                     /locus_tag="K0W68_002041"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBI3073597.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16773..17171)
                     /locus_tag="K0W68_002042"
     CDS             complement(16773..17171)
                     /locus_tag="K0W68_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBI3073598.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17411..19825)
                     /gene="gyrB"
                     /locus_tag="K0W68_002043"
     CDS             complement(17411..19825)
                     /gene="gyrB"
                     /locus_tag="K0W68_002043"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBI3073599.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19854..20927)
                     /gene="recF"
                     /locus_tag="K0W68_002044"
     CDS             complement(19854..20927)
                     /gene="recF"
                     /locus_tag="K0W68_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBI3073600.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20927..22027)
                     /gene="dnaN"
                     /locus_tag="K0W68_002045"
     CDS             complement(20927..22027)
                     /gene="dnaN"
                     /locus_tag="K0W68_002045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBI3073601.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22032..23435)
                     /gene="dnaA"
                     /locus_tag="K0W68_002046"
     CDS             complement(22032..23435)
                     /gene="dnaA"
                     /locus_tag="K0W68_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBI3073602.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23732..23812
                     /locus_tag="K0W68_002047"
     CDS             23732..23812
                     /locus_tag="K0W68_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073603.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24042..24182
                     /gene="rpmH"
                     /locus_tag="K0W68_002048"
     CDS             24042..24182
                     /gene="rpmH"
                     /locus_tag="K0W68_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBI3073604.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24199..24558
                     /gene="rnpA"
                     /locus_tag="K0W68_002049"
     CDS             24199..24558
                     /gene="rnpA"
                     /locus_tag="K0W68_002049"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBI3073605.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24522..24779
                     /gene="yidD"
                     /locus_tag="K0W68_002050"
     CDS             24522..24779
                     /gene="yidD"
                     /locus_tag="K0W68_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBI3073606.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24782..26428
                     /gene="yidC"
                     /locus_tag="K0W68_002051"
     CDS             24782..26428
                     /gene="yidC"
                     /locus_tag="K0W68_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBI3073607.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26534..27898
                     /gene="mnmE"
                     /locus_tag="K0W68_002052"
     CDS             26534..27898
                     /gene="mnmE"
                     /locus_tag="K0W68_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBI3073608.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6749 a         7582 c         7146 g         6564 t
ORIGIN      
        1 cctcatgatt ctcattctga cgtgccagag cacgattgaa aacgaaacga catgcgcctg
       61 cgaagcgcct catttcacgc tcctgttgac caccgggtct taactggaat ttaaatgctt
      121 gtagtcgttt cacaggccaa atgatacttt ggtctgtgaa aaaagaaacc gatatccggc
      181 gtggaaggca ttgtgttttc ctgatgcatg ttcacctggt ctttgtcacc aaataccgac
      241 gtcagatttt tgactatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg
      301 ctgattttga agctgaactg gtttaaatgg atggcgaacc agatcacgtc catttgttaa
      361 ttaactatcc tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa ggcgtatcgg
      421 gtaggttact gcgacgagat cgaccagata ttgcagtcag gtattactac aaaggcgttt
      481 tgtggagtcc tggctatttt gccaatagct gcggaggtgc gccaatatcc gtcatccgcc
      541 aatacattga acaacagcaa acacctggtt aggtggaaaa ccgcgcctta tatccccgcc
      601 ctgaaggacg gggttttacg gcgcaccgga taaatttggt tatgcgccgt cagccgtgct
      661 aaagaatacg gaccagcacc caacggatgc cagtccacac aattaacgta actctggcca
      721 gtacgctttg ttggcctcca gcaactcatc cagaatagct ttggcgaccg aggcgctcgg
      781 taccgttttc gataacgtaa tcgcctgcca caggtgctga tatgaacgct gttcccaggc
      841 gtccacgacc agtttttcca ccgccacttg ctgactcatt aaccctttct gaaactgcgg
      901 gatatcgccg accaccagtg gttctggtcc gttgtgcccg accagacacg ggatctcgac
      961 catcgcttca tcatcaaagt tatggattgc cccgttgtta ggcacaatca gcaacatccg
     1021 ctcctgagtg ttgaaggcaa ttgccgccgc cagatcgacg atgtatgacg catgttcgtc
     1081 aatctccagc ttggccgccg ctgaatttcc tgccgctgta atggcgcggc aagcatcgaa
     1141 aacctgtttc tcgcgatgtt ccattcacct cattcgcgcg ggtatgttca gggttggaat
     1201 gctgaacaac ataatccggg aagagataat atttcagata ggtgttcggc aatgtatccg
     1261 ggtccagtgc ctggacatcc cgcgcttttg cataggtgtc gttccagctc gcttcagtat
     1321 gtgtatcttg ctgcggtgga acataaccgt gcttcgagac atattgccgc agctggggca
     1381 tcaggtcgtt gccctgcaaa tcttcaattg atgtccacca gccaaagtgg ttcaggccgt
     1441 agtagcgcac gcgcatctgt ttgcgatctt gcagcccaac aatttgcgcc atccggcttt
     1501 caataccgat tggcatgtca cagatgttga ggattttcgc attcgggcgc agacgacgcg
     1561 tggcttctgc gacaatggct gccgggttgg agtagttgag catccaggca tttggtgaat
     1621 atttttccat ataatccacc agttccagga cgccgccaat ggaacgcatg ccgtacgcta
     1681 ttccgccggg tccgcaagtt tcctgaccaa caacaccgtg gcgcagtggg attttttcat
     1741 ccagttcgcg catcgggtac ttgccgacgc ggatgtgcgc catgacaaaa tcaacgtcgc
     1801 tgaacgccac ttcaggatcg gtggtgtaac taaacgcaat gtccggcgct ttctctttaa
     1861 ggatgacttt acaggcttcg gcaatcactt cctgccgcgc gccatcgtta tcataaaatt
     1921 tcagtgcacg aagcgggaaa cgctcctgat tcgccaggag catcaacacg atgcctggcg
     1981 taaaggtgct tccaccgcct gcgacaacca ctgagaattt ggtcattata ctgcctccgt
     2041 aatggcaaca ttttctgctg attgatgaga attaattaag ctatcgagct gttcacgcag
     2101 ctgggataca tgcagaccaa tgatcacctg aatggcatcg ccactacgga agacgccgtg
     2161 cgctcccagc tttttaaaga cttcgtcatc cagcgtttgt gacatgtcat gcagtgcaat
     2221 gcgtagacgc gtcgcacaat tgttaatgct ggagatattg ccgaccccgc ccagggcttg
     2281 caggataccg gcagcctgat ccagctcttt ttttggctct gccgcggtgg tttggcctcg
     2341 cgaggctttg tattcggctt ttgagtagag tttcacttcc gcatcttcac gtcccggcgt
     2401 gcacatgttg aactgcagaa tcagggtgcg gaaaaccacg aagtacagca gggtaaagca
     2461 caacccaatg gcgatttggg tcagcatcat atccgcgtgg ttgctgaaca tcgggatcca
     2521 gttttgcggt aaaacctggt caatcagacc tccgcccatg ttgcccacca caccaaagag
     2581 atacatcacg gtcgacattg aggccgcaag cacagcgtgt accgcaaaca gcaacggtga
     2641 aatgaacagg aaggtaaatt ccagcggttc ggtaattccc accagcatgg cggttaaggt
     2701 tgcgggaatc agcaagcccg ccacttttac ccgattttcc ggtgcggcag tgaagtacat
     2761 cgctaaagaa atgcccacgg caccaaagat ttttgagtta ccgtgcaggg caaaaccgcc
     2821 ttccgggaac aacgatttca gcggctcggc gctcaaactg aactcttgca gatgctgcgc
     2881 ccagtacatc tgaatgccgc cttcaacagc tgccggaccc aagataaacg gtccgtagat
     2941 gaagtggtgt aaaccggttg ggatcagaat acgttcgagg aaggtgtaaa cccagacccc
     3001 aagcgcaccc gccgaacgca ggaacgcttg cagagattca atccccgttt gtacttttgg
     3061 ccagccgagc aatgtcagcc aggcacaggg gatcatcacc aggaaggcga taatcaccac
     3121 ataagacgtc ccctggaaaa tgccgagaaa aaccggcagt tttttatcga acagacggtt
     3181 atgcagcgcc gtcacaatgc cggaaatgat aattgcgccg ataatgctgg tatcgagggt
     3241 tttaatcccg gccatcattg tcagaccgct acctgccacc gcgtcctgag tgaaatcgac
     3301 gccgaagtag cttccccagg tcattcccat cgcgttgatg aaatagttcc aggtcaggaa
     3361 actcaccatc accgccagac aagcacgtcc ctgcgcttgc ttagcaaggc caatgggtaa
     3421 accgacagca aaaatcagcg gcatattacg gaataccgtc caaccgccct cttcaataat
     3481 gtgtacgatt tgcgcgaata aactgttcgg atcggtcagt gattccccga caaacatcgg
     3541 gttttgcagc aagatggcaa gacccaccac aatcccggcg aagggaaaca gcagcactgg
     3601 cgtaaacatc gcgccgccaa agcgttgaat ttgactgagc atttccacat cctcttttct
     3661 caattctgta agtaggaagc ctgattggtt atgcggcagg cctggagcaa ggataggaag
     3721 atgcgatcgg cagaacatga cgacccaatg ggattcgtga tcgctttcat gcttttcgtt
     3781 gggcggtaga gatctacgtt ttcgcgaaaa agtagacatc tgtgagcaca ggtacacagc
     3841 atcgaacaca tctttaaaaa aaagatgttt tttcaatcga ttaagcagaa cttgtgtgcg
     3901 cattacccgg gcttgcaggc aaaaaagaga tctagagatg atctacaaaa gcattgcgga
     3961 gcggttaaga attcgactta actccgcaga tttcacgcta aacagccttc ttccgggtga
     4021 aaaaaagctg gcggaagagt ttgcggtatc gcggatgacc atccgtaaag ccattgacct
     4081 gctggtagcg tgggggctgg tggtccgccg ccacggtagt ggcacttacc tggtgcgcaa
     4141 agatgtactg catcaaaccg ccagtctgac cgggctggtg gaggtgttaa aacggcaggg
     4201 aaaaactgtc accagccagg tgctgatttt tgaaatcatg cctgcgcctc cggccattgc
     4261 cagccagtta cggattcaaa tcaacgagca gatctacttc tcccgtcgcg ttcgttttgt
     4321 ggaagggaaa ccgctgatgc tggaagacag ctatatgccg gtaaaactgt tccgtaatct
     4381 ttcgctgcaa catctggaag ggtcgaagtt tgaatatatt gaacaagagt gcgggatttt
     4441 gattggcggt aattatgaaa gcctgacgcc ggtgctcgcc gatagactgc tggcgcggca
     4501 aatgaaggta gcggaacaca cgccactgct gcggatcacc tcgttgtcat atagcgagag
     4561 cggggagttt ttgaattatt cagtgatgtt cagaaatgcc agcgaatacc aggtggagta
     4621 ccatttacgg cgactccacc cggaaaagag ttaaccgata ctccagaaga gcaccgccag
     4681 taattggggg gtgataatgc gcaggaacat cactaacgga tagacagtgg cgtaagagag
     4741 cgccgccgca ccgctagttg gatgaagatt attagcaaac gccagcgccg gaggatcggt
     4801 catggaacct gccagcatcc cgcacatggt caggtaattc attttggcta acatccgcgc
     4861 cagaatgcca acagtaatca gcggaacggc ggtgatcagg gcaccataac caatccagct
     4921 tagcccttcg ccattgacca gggtattcac aaaatcccca ccagatttca gaccaacgac
     4981 cgagaggaac agcacgatcc ccagctcccg cagcgcgagg ttggcacttg gcggcataaa
     5041 ccagtacagc ttgccgatac tgccgatacg cccgaggatc aacgccataa tcagcagacc
     5101 gcccgccagc cccagtttca acgcggccgg gaatcccggc acaaagacgg gaatagaacc
     5161 taacaatacg ccaagcccga tgccaataaa cactggcagc atctgaacct gttgcagttt
     5221 ttgctgcgca ttccccagca cattggcaac ggcatcaatt gcggacggac gccccaccag
     5281 gttgaggata tcgccgaact gcaggctgat atcgccactg gcgaccagtt cgaccccggc
     5341 acggttcagg cgcgagataa caacgtcata gcgttcttta aagtgcaggt cgcgaatacg
     5401 ttttccgagc acgttttcat tggtgaccac cacacgctcg acgcgcaaat cagtgtcttt
     5461 cgtggacagc gaagtatcga cctcctgacc aatcaccagt tgcgcattat gtaaatccgc
     5521 tggctgaccc accagatgca gcaaatcgcc cagttggata atggtatctg gcgaaggaac
     5581 ttttagggtt tcttcgcgtt tcagacgcga gcagataatt ttgtcgccgt tgagaatcgg
     5641 tacatcttta atggctaaat catgcaggtt agggttctca acgcgaatat tgatagtctt
     5701 gatcagcgcg ccgccattgg tgcgtgaaga ctcgtgctgc tgagcttctg tctcgacatt
     5761 gacgcggaaa ataacccgca acatccacat ggtgaacaaa atcccgcaaa tgccgaatgg
     5821 atacgccatc gcgtaactca tccccatctg atcgaccatt tccattggtg tacccaggtc
     5881 gcgcaaaatc tgctgccctg cccccagcgc tggcgtattg gtaaccgcac cggagaaaat
     5941 ccccagcact accggcagtg gaatatcaaa cagtttatgc aggatggcgg taaccagacc
     6001 accgatgatg acgatcagaa cagcaaacag gttgaggcgt aatccggaga cgcgcaatga
     6061 ggcaaagaag cccggcccta cctgaatccc gatggtataa acgaacagga tcaggccaaa
     6121 ttcctgaata acatgcagca tatcgctact taatgtcatc cccgcctgag aaacaaaatg
     6181 gccgacgatg atcccaccaa acagcacgcc gccaatacct aatcctatgc cgcgaaattt
     6241 gacgttgccg ataaacaaac cgacgactgc caccaaagcc agaatactga ccgttaatgc
     6301 tatatcactc atcgtccatt tctcttgaga taacatttta attgctacgg attatggctg
     6361 aatcggtgaa gcagatttgc cttatagcgc acattattac gggaaagaac gtgccgaaat
     6421 atcttaaaca gtcgaaattt aacgcattgt tgaagaagaa agacaaaaaa agccccacca
     6481 gaatggcggg gcaaagagaa tagctagtta gctatttaac gcgggacgtt cgctgatagc
     6541 gatacgctgc gctgcgatgg gttcaggctc attacgaatt aaatcaatat gcagtaaacc
     6601 gtttacgaaa gttgcgccag agacttccat attttcagcc agcgtaaagc tcaggctaaa
     6661 tggctgattc ataagccctt gatgcagcca ttttttctct tcttttggct gctccggcgt
     6721 gccttttacg ctcaggcgcg taccttccag ttgaatctct aaatcttcct gacggaaacc
     6781 tgccagcgca agggtaatgc ggtagtggtt atcgtcgctt ttctcaatgt tgtacggcgg
     6841 gaagctctga ctttcaccgg cgttttgcag tgcgttggcc agtttgtcaa aaccgatcca
     6901 ttgacgcatc agtggggata aatcgaagtt acgcatagtc atttctcctt ctaagaagcg
     6961 agtaagtacc tgcaaatccg aagattcgca tatgctccct gacggcgagc atggagatgt
     7021 caggccgcgc caggcggcct taaggaatta gttgatttcg atacggcgcg gttttttcgc
     7081 ttccggaatc acgcgttcga gatcgatata cagcaaacca tttaccaggt tagcgccacg
     7141 aacatgaatg ttctcagcta actggaattt gcgttcaaag ttgcgttcag cgatgccctg
     7201 gtacagatag gtgcgctctt tttgttcgtc ggcgtgagca cctttcacca ccagcagatt
     7261 atcctgggcg gtaatttcca gttcgctctc agcaaaacca gccacagcga tagcaatgcg
     7321 gtaatggttt tcgtctacca gttcaacgtt atacggaggg tagccgccat tactctggct
     7381 ctggttgttt tctaagtggt taaacaaacg gtcaaatcca atagcagaac ggtaaagcgg
     7441 ggataaatca aagttacgca taatcaatag ctcctgaaat cagcgagaat gtaagacctt
     7501 ccacaatgga caggtcaggt agccagaaca cccatcaggc gcgtcctcat cgactacgat
     7561 gtaaaaatgg gtctggaaat gaacttttca agccttatca gcggactttt ttttgcagtt
     7621 tatggtctat tgcatagact gagggggcag cacgaaacgt taaagtgtga tagccgccac
     7681 agagcgacgt aatgatgatg ttattttcag caagggatcg ctataactca tcaggccaac
     7741 acccacgatc aacagatgaa tttattatga taagaaatgt gttgttagcg ttcatgatat
     7801 gcagcggaat ggcattactc ggaggatgct ccagcgtgat gtcccatacc ggagggaaag
     7861 aaggaacgta tcccggcacc cgcgccagcg ccaccatgat tggcgatgat gaaaccaact
     7921 ggggcactaa gtcgctggcg attctcgata tgccgtttac cgctgtgatg gacacgcttt
     7981 tattaccgtg ggatgtgttc cgcaaagaca gctcggtgcg ctcacgcgtt gaaaaaagcg
     8041 aagccaacgc gcaagccacc aatgcggtta ttccaccggc cagaatgcct gataactgat
     8101 ttagcgatca gtttcggtga tccacagctg gccaccttcc atccacgcca gccattgacc
     8161 atccggcgag aagaccacgg cgtccgcaga aggtggattg gcgtggtttt cggttaaata
     8221 ctcaacctcg ccgctttgcg catgggcaca agcaattcga ttatccagca caaagcccaa
     8281 ccattctcct gacggatgcc agttaaatgc agactgaata tccgttttgt tatgggttaa
     8341 ctggcgcggc tcgccgccct gtggcgagat aagccacagt tgcacaatgc cgttatcatc
     8401 acgcattaaa aacgcgattt gcgtaccctg cggattacag cgcacccagt ggcgcgggac
     8461 gttgactaac cccggataag cccgatggtg ggtaaaggtt aaacgtcgct gcacgacgcc
     8521 acgcggtggc gcgggcaggg tggtttccgt tccacttaac ggcgcatcac ctgccgcttt
     8581 ccagccagct tcatcttgcg gcaactcaac gataaacagc tccggcactt tctcaccctt
     8641 tggcgaaagt gtgtcgccaa taaacgccag cgcgtgattt cctacccatc cttcttcata
     8701 agcacgattg atttcatcac tgccaggctg cggcgtgggc gtggttttac tcaccagcac
     8761 acaccagtgg ctgccgctgt attcacgcgg atgctgcttt tgtacgttga ccggaccaaa
     8821 cggcgcagca acaccgacgt ttcgcaaatc cagcgccgga tcgagttcat gcattacatg
     8881 gtcgttatag gtaaagctca ccctttcacc gttcgggcta aagacatgca catggctgcc
     8941 gccgcgcagc gctcctggcg tatacggcgc ggtaatatcc attgcatcga gattgctcac
     9001 cttgccgcct tcagcaatga ctccgcgccg atgatggaag tcgtaatgcc atgtttcatc
     9061 aggattttcc gggccgtgaa taaagacata tttctctgac ttcggatgaa cggtcaccac
     9121 gccgacgtgt gcgccctgtg acgcacagta gataacctcg acctcaccgg tatggatatt
     9181 cacacgctca atggtctcgc cggtaaacga cgccccagaa ggacgcacgt caaataccag
     9241 ccactggctg tcgggcgtcc aggtattggt attggtgagc aggtgattac ggggagcaaa
     9301 ggttatctgt ttcatggcgc ggtcctgata caaaacgggt ccagccatca tacttcacaa
     9361 ggacttcact ttcagacaat agtcttttgc catcgtgatg aatgtgagtg aaaagatgga
     9421 acatttcgac gtggcgatta ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt
     9481 agccggcaaa atgcaggtga tcgcgctgga taaaaagcac cagcatggta ctgaaggttt
     9541 cagcaaacct tgtggcggtc tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg
     9601 gctgacgctt cctgtcgatg tgatcgctaa tccgcagatt ttcagcgtca aaaccgtcga
     9661 cgtcgccgca tcgctcacgc gtaactacca gcgaagctat atcaatatta atcgccatgc
     9721 tttcgacttg tggatgaaat cgctgatccc cgccagcgtt gaggtttatc acgacagcct
     9781 gtgtcgaaaa atctggcgtg aggatgataa atggcatgtc atttttcgtg cagacggctg
     9841 ggagcagcat attaccgccc gctatctggt cggtgccgat ggtgccaact cgatggtgcg
     9901 gcgacatctc tacccggatc atcaaatccg taaatatgtc gcaatccagc agtggttcgc
     9961 agagaaacat ccggtgccgt tctactcctg catctttgat aatgcgataa ctgactgtta
    10021 ttcatggagt atcagcaaag acggttattt tatctttggc ggtgcctatc caatgaaaga
    10081 cggtcagacg cgtttcacga ctctgaaaga gaaaatgagc gcctttcagt tccagtttgg
    10141 caaagcggtg aaaagcgaaa aatgcacggt gctgtttccc tcacgctggc aggattttgt
    10201 ctgcggtaag gacaacgcct ttctgattgg tgaagcggcg ggatttatca gcgccagctc
    10261 gctggagggg attagctatg cgctggatag cgcagacatt ctgcgttcgg tgttactgaa
    10321 gcagccagag aagctcaata ccgcttactg gcgcgccacc cgcaaactgc gtttaaaact
    10381 cttcggcaag atagtaaaaa gccgatgcct gaccgcaccg gctttaagaa agtggattat
    10441 gcgcagtggt gtggcacata ttccacagtt gaaggattag ccaacgcgct tcacatcgcc
    10501 caccagcagg atataagaga gcgcgccaat caacgcgacg gcggagatat aaaccagtgc
    10561 aggtgcgaaa ccgtaaccct gcgccaggta ccccaccacc agcggaacgg tgatgccgcc
    10621 cagaccaccg gcgaagttaa acacgccgcc ggttaaacca atcaggcgca tcggtgccag
    10681 agaagaaacc agcgaccagg taattgaagc aaaaccgtta ccgaagaatg ccagcgccat
    10741 caggcacata atcatcatcg gatcgttagt gtagttagcg cccataatgc aggtggagat
    10801 cagcaagccg cagataatcg gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac
    10861 cagcagatcc gctacccagc cagagagcag gacgccgaca aacgccgcga ggaatggcac
    10921 cgtggtcata aagcctgctt tcagcgccgt gattcctttt tcctgggtta aatagttcgg
    10981 gaaccaggtt aagaaaaacc acagtgtaga agccaccgca aattgcccaa gatagacgcc
    11041 gatcagttta cgatggaaca ccagtttcca gtctttggct gttaacggct gacgtgcctc
    11101 tttcttcact ggcgcatcac catccaccag accgccgcca tcacgaatgt aatccagttc
    11161 agctttgctg atgcctttgg tcaggcgcgg cggctgataa accttaaacc aaatcagcga
    11221 ccagataatg ccgataccac cagtcacaat gaacacccag tgccagctca acatctcctg
    11281 aatccagatc agcagcggag tcaaaaacgc cagaccgaca aactgaccag acgtataaaa
    11341 accaacggcg gaggcgcgtt catgttccgg gaaccagctg gtcaccatcc ggttattggt
    11401 cgggaaagcg ggcgcttcga aaataccggt tatcgcacgc agaccaatta atgacattaa
    11461 tcccgtggca aagccctgga ataaagtcgc cactgaccag ccaaatatcg caataaaata
    11521 agtcacgcga gaacctacgc gatctaaaaa ccaaccgccg gggatctggc atagcgtata
    11581 aagccaggcg aaggccgaaa atacatagcc catttccgct ttcgtaatgc cgaactcttc
    11641 ctgaatatgg gcggaagcca cggccaggtt agcgcggtca acataacaaa tgaccaccgt
    11701 aataaagatc atcaccagcg tcagataacg ccgacgcccc ggctttgctg cattaacggg
    11761 aatatccata gcgagctttc tccagatttt gggcatagcg aagccgctca ccatgccctg
    11821 taatttacag agggttatat ttttgtattg ctgttttagt gcccgatgag gggcttacgt
    11881 ggcaggaatt accactctgc tacgctgtta tcttcatgac gccagagtgg attacgccag
    11941 tccggggcat ttttactgaa ctcaataact ttagcttcgt cgatttccac gcctaagccc
    12001 ggtttcgtta gcggtttaaa gaagccgcca accatgctga agtcttcttt gtttttcaca
    12061 aagtcgagta actccgcgcc tttgttgtaa tgaattccca tactttgttc ctgaagtacc
    12121 gcgttatagg aaacaaagtc gatatgcaga caagccgcca gtgcaatcgg tccgagcgga
    12181 cagtgcggcg caagggtcac gtcataggct tctgccattc cggcaatttt gtagcattcg
    12241 gtaataccgc ctgcatggga gagatccggt tgtagaatcg aaataccgcc tgcctccagc
    12301 acgcgtttaa aatcgaaacg tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt
    12361 tgtgccgcca atttcgggta gtattcggct tgttccgcca gcaccggctc ttcaataaac
    12421 agcgggcgat acggctccag ttctttaatc agcactttcg ccattggcgc gctgacgcga
    12481 ccgtggaaat caagaccaaa ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg
    12541 gtgttgactg ccgcatctac cgcgcgggag ttatcaatta gccccagttc ttcacaaccg
    12601 ttcagtttga aggtatcgaa gccgatttcg cgcagggttt taataccgtc gatcacatcc
    12661 gccggacgat cgccgccgac ccaactgtag gctttaattt tgtcgcgaac caggccgccc
    12721 atcagttgcc agaccgacgc attcagcact ttacctttga tatcccataa cgcctggtcg
    12781 atcccggcaa tggcgctcat caggattgga ccgccacgat aaaatccggc gcgatacatc
    12841 acttgccata agtcattgat gcgctatgga tcctgaccaa tcaaatagtc acccagctcg
    12901 tgaactgccg cttccaccgt gcgggcgcgg ccttcaatca ctggctcgcc ccagccgacg
    12961 acgccttcat cggtttcaat tttcaggaac atccagcggg gaggtaaacg atacgtggta
    13021 attttggtga ttttcattgc actgcctctc gatacgcctt aacaaatgct gctgcctgct
    13081 gcgcggtgcg ttctacggac tgcccggcgc gatagagatc gctgcctaag cccgccccag
    13141 cacaacctgc gtctatccac tgcgccaggt tttctggcgt cacgccgcca acggcaaaga
    13201 ctgcgatgtc cgatggcaat accgctttta acgctttgat gtattgcgga ccaaaagccg
    13261 atgacggaaa tattttcagc gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg
    13321 ccgtcgcgca gccggggcag acggtcatgc cgtagcccac cgcacggcgg atcacttcac
    13381 tatggatatt gggcgtaacg atgagctgac agcccatcct ggcgagcgca tcgacctgtt
    13441 caggtttcag taccgtacct gcgccaatca acgccttgtc gccatacgca tcaacgatgg
    13501 cgggaatgct ttgctcccat tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg
    13561 cgtcaatcac cgcgccaaca tgcgccagcg cctcgtcggg cttaatgccg cgcaaaatgg
    13621 cgatcagcgg aagtttagtt tgccactgca tgagcgatgc tccttatacc agcctgaaat
    13681 gccgtgtcgc ccgccaccgc cgtcacgtcg caacccatcg cctgaaaggc ttgctggtag
    13741 cgcgcggtca acgatgttcc ggcgacaagg gtgatggcgt gttgatgggc cacatagtcg
    13801 cgcatactgg cgacctctgc gccaatcaac aaaccagaga gaaattcact gacctgttca
    13861 cggggaagtg ttcccagcac atgcgaggca cgaacttcaa aaagctgcgg caatatggcg
    13921 ggcgtattaa gaccacgctc aaggccagcc gtgaaggcat cggcagagtt ttcctgcggc
    13981 ggcaaacctg caccaatcaa tgagtgattt aacagtaaat gatgtaattc accggtcatc
    14041 acggtgcgaa aatcgttgat ttgctggcta tcggcctgca cccatttgca atgcgtaccg
    14101 ggcatgacat aaagagagga aggagccaga gctcgcgcgc cgatcaattg agtttcttcg
    14161 ccgcgcatca cattgtggtt atcatcatga gagacgcata atccgggaat aatccagata
    14221 ttgtcgccaa cagacgttaa ttgttcgcca atagacgaaa aatgggcagg aacagataaa
    14281 tacggagcaa ctttccagcc gacattgctg ccgaccattc ctgccattac cactggcgta
    14341 ttctcttcac gccagtcggt cgtgacttct gctaacaccg cagccggaga ttttccgttc
    14401 aggcgcgtga cgcctgcttc tgattgcctg ctctccaggc agtggtcgcc ctgataaagc
    14461 caggcgcgca gattggtcga tccccagtca attgcgatgt agcgagctgt catgtgattt
    14521 cctttaacct tcgtgtcgag ctggcgatca tggtaagcgc cgcctgctct gccgcatcgc
    14581 cgtcctgatg ccgtatcgca tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt
    14641 tggcctcatc gcccatccag gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa
    14701 tgctaagttg ctgtaacacc gggttatgca ccgactgcag caccgcctcg tggtagcgaa
    14761 tatccgcttc gttaaacgct tcgcggtcct gattgttggc aatcatctcg ttcagcgccg
    14821 attcaatctg cgccagatcg ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg
    14881 gttccaccag atttcgcact tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt
    14941 ccagcaccca ttgcagtacg tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa
    15001 acgcgccgcg ataacgtttc atttcaatca gccgtttcgc catcaacgaa cggaacacct
    15061 cacggatgat gttgcgcgag gttgcaaact cctcacagag ttccgcctca gccggaagcg
    15121 gcgagcctgg tacgtatttg ccgtgaacga tctgtttacc cagcgtaatg acgatgcgat
    15181 cggttttatt gagagtcatg gagagtcctt gtgctctgta tgttcttctc tactttaccc
    15241 cgatcgatgc ataatcagga aagtatcaat cgcagtcaga caccatcacg gggctatcgc
    15301 aggcaaagga tagtaatgga acaaaaggct atgtttttgt aggggaatcg ctggattacc
    15361 ttatcacaga tggtgccgat gacatcgtta agatgctcaa ggatccagct cttaaccggc
    15421 acaatattca ggttgctgat gacgcaagat ttgttttaaa tgcggggaaa aagaaattta
    15481 ccggcacaat atcactctat tacaactgga gcacagaaga agaaaaagca cgagtgacac
    15541 aatatcgttt tgcttgtggt gttcagcact gtacccggac agtagaaaac ctggaaggta
    15601 ctatccatga gaaaaataaa aacatggatt actcaaacgt aatggctttc taccatccat
    15661 ttaaagtgcg attttatgaa taccattcac ccagaggcat tccggatggt gtttccgcag
    15721 cattactgcc agtgactgtt acgctggaca tcattactgc accgctgcaa tttctggttg
    15781 tatatgcagt aaaccaataa tcagtaagcg ggcaaacgcg tttatgctgt ttgcccgccc
    15841 acagattaat tcagcacata cttctcaata gcaaacgcca cgccatcttc aaggttagat
    15901 ttggtgacaa agttcgccac ttctttcacc gaaggaatag cgttatccat cgccacaccg
    15961 acgcctgcat attcaatcat tgcgatgtcg ttttcctgat cgccaatcgc catgatttct
    16021 tccggtttaa tacctaacac gtcggccagt gatttcacac ccgtaccttt gttaacgcgt
    16081 ttatcgagga tttcgaggaa gtacggcgca cttttcagca cggtatattt ctctttcact
    16141 tcctgcggaa tacgcgcgat agcctggtcg aggatggcgg gttcatcaat catcatcact
    16201 ttcaggaact gggtattggg gtccattttc tccgcttcgc agaacaccag cggaatagtg
    16261 gcaacgaaag attcatgcac tgtgtagtag ctgatatcgc ggttggcggt gtacagcgtg
    16321 gtgcggtcca gggcgtggaa atgagaaccg acttcgcgag agagtttttc caggaaacga
    16381 tagtcgtcat agctgagagc agtttgcgcc acggtgctac catcagctgc cttctgtact
    16441 agcgcgccgt tataagtaat gcagtagtcg cccggctgtt ccatatgcag ctctttcagg
    16501 tagttgtgca cacctgcata cgggcgaccc gtcgttagca cgacattcac gccacgggcg
    16561 cgagctgcgg caatcgcatt tttaacggcg ggtgaaatgg tgtgatcggg cagcagaagg
    16621 gtgccatcca tatcgatagc aatgagtttg atagccatga gttccccagg tagattggtt
    16681 cctgacccat gctaacgcga ttccgctcaa aaatcagtac aacacccgag ggaaaagggg
    16741 gatgcaacgc gggttgcatg ctccccattt gcttagcgga agagtttccc tttcagcagt
    16801 tccatacctg cggaaagcag atcgttattg gcttgtggtg acacttcacc ttgcggtgag
    16861 agcgcatcaa taatcttcgg caattgctct gccagtaaac tggaagctgt actggtatcc
    16921 acgccaagtt tttgcccgag atcggacacc gcatttgtgc cgagcgccga ttccagttgc
    16981 tcgccactaa ccgattgatt gccctgttga ttactcagcc aggttgagag aatggcccct
    17041 aagccgccac tttgcagttt ttccagcagc acctgaatgc cgccctgctc ctcaacccaa
    17101 cttaaaatag cctgatattt ccccgcatcg cctttcagaa aggcaccgac aacttcatca
    17161 aaaagcccca tgataatcac ctgtaaagcg ttacgtgttg acccaaaaag tatagatttg
    17221 cgggatgata attgcggatt gcagaaataa aaagggcgga gatgatctcc gcccttttct
    17281 tatagcttct tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat catgaaaatt
    17341 caatacattg caagattttc gtaggcctga taagcgtagc gcatcaggca cgctcgcatg
    17401 gttagcgcca ttaaatatcg atattcgccg ctttcagggc gttctcttca ataaacgcac
    17461 ggcgcggttc aacggcgtcg cccatcagcg tggtgaacaa ctggtcggca gcaatcgcat
    17521 ctttaacggt aacgcgcagc atacgacggc tttccgggtc catggtggtt tcccacagct
    17581 gttccgggtt catctcgccc agacctttat aacgctggat ggagaggccg cgacgggact
    17641 ctttcaccag ccagtccagc gcctgctcga agctggctac cggctgacga cgctcgccac
    17701 gttcgataaa cgcatcttct tccagcaagc cacgcagttt ctcacccagc gtgcagatac
    17761 gacgatattc gccaccggtg ataaactcgt gatccagcgg atagtcagta tccacaccgt
    17821 gggtacgcac gcgaacaatc ggttcaaaca ggttttgttc ggcattggtg tggacatcaa
    17881 acttccactg gctgccgtgc tgttctttgt cgttcagttc gctgaccagc gcgttcaccc
    17941 agcgggtaac ggtctgctca tcagagaggt cggcttccgt cagcgtcggc tgatagataa
    18001 gctctttcag cattgctttc ggataacgac gctccatgcg gttgatcatt ttctgcgtcg
    18061 cgttgtactc agacaccaat ttctctaacg cttcgccagc caatgccggt gcgctggcgt
    18121 tggtgtgcag cgttgcgcca tccagcgcga tagagatctg gtactgatcc atcgcttcgt
    18181 cgtctttaat gtactgttct tgcttgcctt tcttcacttt gtacagcggc ggctgagcga
    18241 tgtagacgtg accgcgttca acgatttccg gcatctgacg atagaaaaag gtcaacagca
    18301 gcgtacgaat gtgcgagccg tcgacgtccg catcggtcat gatgatgatg ctgtgataac
    18361 gcagtttgtc cgggttgtac tcgtcacgac cgataccaca gccaagcgcg gtgataagcg
    18421 tcgccacttc ctgagaagag agcatcttat cgaagcgcgc tttctcaacg ttgaggattt
    18481 tacccttcag cggcagaatc gcctggttct tgcggttacg cccctgcttc gcagagccgc
    18541 ccgcggagtc cccttccacc aggtacagtt cggaaagcgc cggatcgcgt tcctggcagt
    18601 ctgccagttt gcccggcagg cccgctaagt cgagcgcacc tttacggcgg gtcatttcac
    18661 gcgcgcgacg cgccgcttca cgggcacggg cagcatcgat aattttgcca accacgattt
    18721 tcgcgtcggt tgggttttcc agcaggtatt ctgccagcag ttcgttcatc tgctgttcaa
    18781 ccgctgattt cacctcagaa gaaaccagtt tgtctttggt ctgggaggag aatttcgggt
    18841 ccggcacttt cacggaaacg accgcaatca ggccttcacg cgcatcgtca ccggtggcgc
    18901 taactttggc ttttttgctg tagccttctt tgtccatgta ggcgttcagg gtacgggtca
    18961 tcgccgcacg gaagcctgcc aggtgagtac cgccgtcacg ctgcggaatg ttgttggtaa
    19021 agcagtagat gttttcctgg aagccatcgt tccactgcaa cgccacttcg acgccaatac
    19081 cgtctttttc agtggagaag tagaagatat tcgggtggat cggcgttttg ttcttgttca
    19141 gatattcaac gaacgccttg atgccgcctt catagtggaa gtggtcttct ttgccgtcgc
    19201 gcttgtcgcg cagacgaatg gaaacgccgg agttgaggaa cgacaactca cgcagacgtt
    19261 tcgccagaat ttcatattcg aactcggtca cattggtgaa ggtttcgagg ctgggccaga
    19321 aacgcaccat ggtgccggtt ttttcagtct cgccggtaac cgccaacggt gcctgcggta
    19381 caccgtgttc gtagatctga cggtgaattt taccctcgcg ctggataacc agctccagtt
    19441 tttgcgacag ggcgtttact accgaaacac caacgccgtg cagaccgccg gacactttat
    19501 aggagttatc gtcaaattta ccgcctgcgt gcagaacggt catgatcact tccgccgccg
    19561 atacgccctc ttccgggtga ataccggtcg gaatgccgcg cccgtcatcc tgtacagaga
    19621 cagagttatc ggcgtgaatg gtgacgataa tttctttaca gtgacccgcg agcgcttcgt
    19681 cgatagcgtt atctaccacc tcgaatacca tgtggtgcag accggtgccg tcatccgtgt
    19741 cgccgatata catacccggg cgcttacgca ccgcatccag ccctttcagg actttgatac
    19801 tggaggagtc ataagaattc gacatcaacg tttctcgctc atttatactt gggttaatcc
    19861 gttattttac ccttttccac ggtaaacatc ttcgaatttt cgtccgacat gtctataacg
    19921 tgttcagcac tgatcgcgct gacaaagacc tgtgattgcg tcgcttttaa gcggctggca
    19981 agcagcccgc gacgctcatc atcaagctca gaggcaaaat catctatcag gtagagacac
    20041 cgccgcccgc tttcacgggt gaggaactct ccttgcgcca gacgtaaggc gcacatcaac
    20101 agcttaagct gcccacgcga taaggtatct tccaccggcg caccgtcggc gcgaatgcgt
    20161 aagtccgctt tatgcgggcc atgcgcggtg taggttagct ggcgatcgcg ttcaaaatta
    20221 cgttccagca cctcagcata ttctgtctct ttctcccagc cgcgctggaa agagaaagtc
    20281 agagaaaact cagggagaaa ttgcttacaa gtatcggcca tatcagccgc gataccggcg
    20341 ctatactccg cgcgccaggt gctgatttgc tccgccagcg ggatcagctc tttatcccac
    20401 gggcgtagct gttcgtaacg tgtcacctgg cgcagcgccg cattgcgctg cttgagcaat
    20461 cgcttgagat tgctccaggc ggtgaaaaat ccgggttcgt tgtgaaagca tccccagtcg
    20521 aggaatgctc ttctgtattt ggggccgccg ttgagtaaag taaacccttc tggcgttatc
    20581 aactgcattg gcatcaggtg cgccagttcc gcgaccttat gcccgtctgt accgtcgatg
    20641 cggactttgc tgtcgccctg tttgtctttg gttaagccaa tcgctgtctc gcgctcttcg
    20701 ccctgtaatc gcccgtggag aacaaacgcc tcctgctcat ggcgaatgac gcgaccaatc
    20761 tgcaaactgc gaaacgcccg accatggccg agcgtataga tggcttccag cacgctggtt
    20821 ttgccactgc cgttggcacc taccagaaag ttaaagccgg gagataaagc gagatccgcg
    20881 gtttcaatgt tgcggaaatc gcggatcaac aagcgggtga gggacattac agtctcattg
    20941 gcatgacaac ataggcagcc gactgtgatg cggcatcttc aatctgcacg ctggaaaccg
    21001 aatcggtcag catcatgcgg acgttttcac atttcagcgc gttcagaaca tccagcacgt
    21061 agctgacgtt gaagccgatt tccatctccg caccgctata ggtaacgtcg aggatctctt
    21121 ccgcttcttc ctgttccggg ttgttggcgg tgattttcag ctggttttcg ctgacataaa
    21181 gacgtacgcc gcggaatttc tcgttagaga gaatcgccgc gcgagcaaac gcctgcttga
    21241 gcagatcgca gccagcttcc agatgtttgt ccgggttctt cggcagaacg cggcgataat
    21301 ccgggaagcg accatccacc agtttggagg tgaagataaa gtcgccaacg tgggcgcgaa
    21361 tgttgttgct gccaatctgt acgcgcagcg gattgtcgcc gccgtcgagc atacgcatca
    21421 gttcaatcac gcctttacgc ggcacgatca ccgaatggct tggcaaagat tgaccaattg
    21481 gcattgaaca gactgccaga cggtggccgt cggtcgccac ggtgcgcagt tcttcgcctt
    21541 cggtttcaaa cagcataccg tttaagtaat agcgaacgtc ctgatgcgcc atagaaaact
    21601 gggtcgcttc aatcagacgc ttcatcgtcg cctgcggcag ggtaaattcg acttcgctct
    21661 gccagtcatc gaggttcggg aaatccgccg ctggcagggt agacagcgaa aaacggctac
    21721 gcccggagcg caccagcatc cgttcacctt ccagctgtac ggcaatttcc gcgccttcag
    21781 gcagaccacg gcagatatca aagaatttgc gcgccggaac ggtcgtcgct cctggctcgt
    21841 gtggctgaac cagcgcaacg cgtgccacca tttccatctc gagatcagta ccggtcagcg
    21901 acaacgtacc gtcagcaacc tgtaacagca gattaccgag aatcggtagc gtaggacgac
    21961 cacctaacgg cccgctcacc tgttgtagcg gttttaataa atgctcacgt tctacggtaa
    22021 atttcatagg tttacgatga caatgttctg attaaatttg aaaaatcttc tttgatatcg
    22081 tggctctctt cacgcaactg ctcgatctta cggcaggcat gaagcaccgt cgtgtggtca
    22141 cgaccaccaa acgcatcgcc aatctccggc agactgtggt tagtcagctc tttcgccagc
    22201 gccatcgcca tctggcgcgg acgcgccacc gagcgggatc gacgcttgga aaggagatcc
    22261 gcgactttga tcttgtagta ctccgccacc gtcttctgaa tattgtcgat ggtgaccagt
    22321 ttttcctgca atgccagcaa gtcgcgcagt gcctcacgca cgaagtcgat ggtgatcgcc
    22381 cgtccggtaa agttggcgtt agcaataacg cggttcagcg ccccttccag ctcacgtacg
    22441 ttagatcgta gacgcttggc gataaagaac gccacttcgc ctggcaaacg aatgtcattt
    22501 tcgtcggcct ttttcatcag gatcgccacg cgggtttcca gctctggcgg ttcgatcgcc
    22561 acagtcagtc cccaaccgaa gcgggatttc aaacgatcct caacgccgtt gatctctttc
    22621 ggatagcgat ccgaggtgag aatgatctgt tgattacctt ccagcagggc gttgaaggtg
    22681 tggaaaaact cttcctgaga tcgttcttta ttagcaaaaa actgaatatc gtcgatcagc
    22741 agtgcatcta cggaacggta gtagcgttta aactcttcga tcgcgttgtt ttgcagggct
    22801 ttaaccatgt cctgaacaaa gcgctcggag tgcatataaa ccactttggc attcggcttg
    22861 cgcgccataa tgccgttacc caccgcatgc agcaggtgag ttttacccag acccgtgccg
    22921 ccataaagga acaatgggtt ataggcaccg ccagggttat ccgccacctg gcgagccgcc
    22981 gcgcgcgcca gttggttaga tttaccttca acgaagttat caaacgtgtg tttgacgttt
    23041 acgttagaac gataggtcgg ttctgccggg gccgggacgt tatcccaacc tgagcgcgta
    23101 gaaggcgcag cacgttgcgg ctgcgtttgc gccacctgtg caggggccgc gacgttgctc
    23161 gtcactgccg cttgtggcgt ttgcgtcacc ggtttggtgc cgacttcaaa acgcagctgt
    23221 ggggcatccg ctccgcagaa actggttagc agtccattga tattattaag gtacttgtcc
    23281 cgtacccaat cgaggacaaa acggtttggc gcgtacaggg ccagcgtgtt atcgctcagt
    23341 tccgcctgca atgggcgtat ccacatactg aattctgtgg ctggtaactc atcctgcaat
    23401 cgggcaagac actgctgcca aagcgaaagt gacacggcgg actccactcg aacaaaagtc
    23461 gataatgact aaggctgaaa cattcatgat tgttgacgta cgtcgaaaag accctgcttg
    23521 agggtgacgc acgaaccgct gtctgcggtt atatgcccga tcaagatcct gcaaaacgat
    23581 cgggaccgcg gatcatagcc taaactgcgc aagagatctt ctgtttctca cagattttcc
    23641 cgatttatcc acaggacttt ccagacctcg ctaagtgtaa acgatcctgc cgcgaggcgg
    23701 gcacgattta cgccgcatat tggaaaattt aatgaccata gacaaaaatt ggcttaatcg
    23761 atctaataaa gatctaggac gatccttgcg ctttacccat cagcccgtat aatcctccac
    23821 ccggcgcgcc atgctggttt tcactggtgt gaggtcgtac attttccctg cgaaaaggtg
    23881 cggaaaagcg cggtaaataa ggaaagagaa ttgactccgg agtgtacaat tattacaatc
    23941 cggcctcttt aatcacccat ggcttcggtg tccatcgttt catttttcgg cggatatcca
    24001 ataaagccat tgaatttatt caagtttagg tagaaatcgc catgaaacgc acttttcaac
    24061 cgtctgtact gaagcgcaac cgttctcacg gcttccgtgc tcgtatggct actaaaaatg
    24121 gtcgtcaggt tctggcacgt cgtcgtgcta aaggccgcgc tcgtctgacc gtttctaagt
    24181 aataaagcta acccctgagt ggttaagctc gcatttccca gggagttacg cttgttaact
    24241 cccagtcaat tcacattcgt cttccagcag ccacaacggg ctggcacgcc gcaaattacc
    24301 attctcggcc gcctgaattc gctggggcat ccccgtatcg gtcttacagt cgccaagaaa
    24361 aacgttcgac gcgcccatga acgcaatcgg attaaacgtc tgacgcgtga aagcttccgt
    24421 ctgcgccaac atgaactccc ggctatggat ttcgtggtgg tggcgaaaaa aggggttgcc
    24481 gacctcgata accgtgctct ctcggaagcg ttggaaaaat tatggcgccg ccactgtcgc
    24541 ctggctcgcg ggtcctgata gccctcattc gggtctatca acgcctgatt agtccgctac
    24601 tcgggccgca ttgtcgtttc actccaacct gttcaagcta cggaattgag gcattgcgca
    24661 ggtttggagt gataaaaggc agttggttga cggtgaaacg cgtattaaaa tgccaccctt
    24721 tacaccctgg tggtgacgat cccgtcccgc ccggaccatt tgataccaga gaacactaac
    24781 gatggattcg caacgcaatc ttttagtcat cgctttgctg ttcgtgtctt tcatgatctg
    24841 gcaagcctgg gagcaggata aaaacccgca acctcaggcc caacagacca cgcagacaac
    24901 gaccaccgca gcgggtagcg ccgccgacca gggcgtaccg gccagtggcc aggggaaact
    24961 gatctcggtt aagaccgacg tgcttgatct gaccatcaac acccgtggtg gtgatgttga
    25021 gcaagctctg ctgcctgctt acccgaaaga gctgaactct acccagccgt tccagctgct
    25081 ggaaacttca ccgcagttta tttatcaggc acagagcggt ctgaccggtc gtgatggccc
    25141 ggataacccg gctaacggcc cgcgtccgct gtataacgtt gaaaaagacg cttatgtgct
    25201 ggctgaaggt caaaacgaac tgcaggtgcc gatgacgtat accgacgcgg caggcaacac
    25261 gtttaccaaa acgtttgtcc tgaaacgtgg tgattacgct gtcaacgtca actacaacgt
    25321 gcagaacgct ggcgagaaac cgctggaaat ctccaccttt ggtcagttga agcaatccat
    25381 cactctgcca ccgcatctcg ataccggaag cagcaacttc gcactgcaca ccttccgcgg
    25441 cgcggcgtac tccacgcctg acgagaagta cgagaaatac aagttcgata ccattgccga
    25501 taacgaaaac ctgaacatct cttcgaaagg tggttgggtg gcaatgctgc aacagtattt
    25561 cgcgacggcg tggatcccgc ataacgacgg taccaacaac ttctataccg ctaatctggg
    25621 taacggcatc gccgctatcg gctataaatc tcagccggta ctggttcagc ctggtcagac
    25681 tggcgcgatg aacagcaccc tgtgggttgg cccggaaatc caggacaaaa tggcagctgt
    25741 tgctccgcac ctggatctga ccgttgatta cggttggttg tggttcatct ctcagccgct
    25801 gttcaaactg ctgaaatgga tccatagctt tgtgggtaac tggggcttct ccattatcat
    25861 catcaccttt atcgttcgtg gcatcatgta cccgctgacc aaagcgcagt acacctccat
    25921 ggcgaagatg cgtatgctgc agccgaagat tcaggcaatg cgtgagcgtc tgggcgatga
    25981 caaacagcgt atcagccagg aaatgatggc gctgtacaaa gctgagaagg ttaacccgct
    26041 gggcggctgc ttcccgctgc tgatccagat gccaatcttc ctggcgttgt actacatgct
    26101 gatgggttcc gttgaactgc gtcaggcacc gtttgcactg tggatccacg acctgtcggc
    26161 acaggacccg tactacatcc tgccgatcct gatgggcgta acgatgttct tcattcagaa
    26221 gatgtcgccg accacagtga ccgacccgat gcagcagaag atcatgacct ttatgccggt
    26281 catcttcacc gtgttcttcc tgtggttccc gtcaggtctg gtgctgtact atatcgtcag
    26341 caacctggta accattattc agcagcagct gatttaccgt ggtctggaaa aacgtggcct
    26401 gcatagccgc gagaagaaaa aatcctgatt cggtgagttt tcgctaaaat aagggcggtc
    26461 agttgaccgc cttttttctt ttcgtagggt ggataagcac cgcgcatccg ccacacaaag
    26521 caacaggaac atcatgagcg ataatgacac tatcgtagcc caggccacgc ctccgggacg
    26581 tggcggcgtt ggcatcctgc gcatctccgg cctaaaagcc cgtgaagttg ccgaaaccgt
    26641 gctgggtaaa ctgcctaagc cgcgctacgc cgattatctt ccgtttaaag acgccgacgg
    26701 cagcgtgctc gatcagggga ttgcgctatg gttccctggc ccgaactcgt tcaccggcga
    26761 agatgtgctg gaactgcagg gtcatggcgg tccggtgatc ctcgacctgc tgttaaaacg
    26821 cattctgacc attcccggcc tgcggattgc tcgccctggt gagttttccg aacgcgcgtt
    26881 tcttaacgat aaactcgact tagcccaggc cgaggcgatt gccgatctta tcgacgccag
    26941 ttcggaacag gcggcccgtt cggcacttaa ctcgctgcaa ggcgcattct ccgcacgggt
    27001 caaccatctg gtggaagccc tcacccactt gcgcatttac gtcgaagcgg caattgattt
    27061 ccctgatgaa gagatcgatt tcctctccga cggcaaaatt gaagcccagc ttaatgacgt
    27121 gattgccgat ctcgatgcag tgcgtgctga agcacgtcag ggtagtttgt tgcgcgaagg
    27181 gatgaaggtg gtgattgccg gacgtcctaa cgccggtaaa tcgagcctgt taaacgcgct
    27241 ggcgggccgt gaagcagcaa tcgtaactga tatcgccgga accacacgtg acgtgctgcg
    27301 tgagcatatc cacattgacg gaatgccgct gcatatcatc gataccgctg ggctacgtga
    27361 agccagtgac gaagtggaac gtattggtat cgagcgcgcg tggcaggaaa ttgaacaggc
    27421 cgaccgcgtg ctgtttatgg tcgatggcac cacaacagac gccgtggatc cggcagagat
    27481 ctggccggaa tttatcgccc gtctgccagc gaaactaccg atcaccgtgg tgcgcaataa
    27541 agccgatatc accggcgaaa cgctgggaat gagtgaagtg aacggtcacg cgttaattcg
    27601 tctctcggca aggacaggtg aaggcgtgga ggtgctgcgt aaccatctca aacagagcat
    27661 gggctttgac accaacatgg aaggcggctt cctggcgcgt cgtcgccacc tacaggcgct
    27721 ggaacaggca gcggagcatt tgcagcaagg taaagcgcaa ctgttaggtg cctgggcggg
    27781 tgaactgctg gcggaagagt tgcgcctggc acagcagaac ttaagcgaaa tcaccgggga
    27841 atttacctct gatgatttgc tggggcggat tttctccagc ttctgtattg gtaagtaacc
    27901 gcgcttacga agccgtattc tgactgtcag atgcggcttc gcttcattgt tagcactcct
    27961 gttattcctc aacccttttt ttaaacatta aaattcttac gtaatttata atctttaaaa
    28021 aaagcattta atattgctcc c
//
LOCUS       DAETQN010000048        27502 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.048, whole genome shotgun sequence.
ACCESSION   DAETQN010000048 DAETQN010000000
VERSION     DAETQN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27502
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.048"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(36..119)
                     /locus_tag="K0W68_002053"
                     /pseudo
     CDS             complement(36..119)
                     /locus_tag="K0W68_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 domain-containing protein"
     gene            complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="K0W68_002054"
     CDS             complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="K0W68_002054"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBI3073609.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="K0W68_002055"
     CDS             complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="K0W68_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HBI3073610.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="K0W68_002056"
     CDS             complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="K0W68_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBI3073611.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5772..6269
                     /gene="msrC"
                     /locus_tag="K0W68_002057"
     CDS             5772..6269
                     /gene="msrC"
                     /locus_tag="K0W68_002057"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBI3073612.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6366..7064
                     /gene="proQ"
                     /locus_tag="K0W68_002058"
     CDS             6366..7064
                     /gene="proQ"
                     /locus_tag="K0W68_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBI3073613.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7084..9132
                     /gene="prc"
                     /locus_tag="K0W68_002059"
     CDS             7084..9132
                     /gene="prc"
                     /locus_tag="K0W68_002059"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBI3073614.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9324..10205
                     /gene="htpX"
                     /locus_tag="K0W68_002060"
     CDS             9324..10205
                     /gene="htpX"
                     /locus_tag="K0W68_002060"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBI3073615.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10251..11314)
                     /locus_tag="K0W68_002061"
                     /pseudo
     CDS             complement(10251..11314)
                     /locus_tag="K0W68_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11491..12282
                     /gene="kdgR"
                     /locus_tag="K0W68_002062"
     CDS             11491..12282
                     /gene="kdgR"
                     /locus_tag="K0W68_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBI3073616.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12425..12664)
                     /locus_tag="K0W68_002063"
     CDS             complement(12425..12664)
                     /locus_tag="K0W68_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073617.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12823..12966
                     /gene="mgrB"
                     /locus_tag="K0W68_002064"
     CDS             12823..12966
                     /gene="mgrB"
                     /locus_tag="K0W68_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBI3073618.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13041..13328
                     /locus_tag="K0W68_002065"
     CDS             13041..13328
                     /locus_tag="K0W68_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBI3073619.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13998..14141
                     /locus_tag="K0W68_002066"
     CDS             13998..14141
                     /locus_tag="K0W68_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBI3073620.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14154..14363
                     /gene="cspE"
                     /locus_tag="K0W68_002067"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14154..14363
                     /gene="cspE"
                     /locus_tag="K0W68_002067"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBI3073621.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14529..15338
                     /gene="rlmA"
                     /locus_tag="K0W68_002068"
     CDS             14529..15338
                     /gene="rlmA"
                     /locus_tag="K0W68_002068"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBI3073622.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15335..15901)
                     /gene="mntP"
                     /locus_tag="K0W68_002069"
     CDS             complement(15335..15901)
                     /gene="mntP"
                     /locus_tag="K0W68_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBI3073623.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16330..16788)
                     /locus_tag="K0W68_002070"
     CDS             complement(16330..16788)
                     /locus_tag="K0W68_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBI3073624.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(16843..17694)
                     /gene="manZ"
                     /locus_tag="K0W68_002071"
     CDS             complement(16843..17694)
                     /gene="manZ"
                     /locus_tag="K0W68_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBI3073625.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17707..18507)
                     /gene="manY"
                     /locus_tag="K0W68_002072"
     CDS             complement(17707..18507)
                     /gene="manY"
                     /locus_tag="K0W68_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBI3073626.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(18570..19541)
                     /gene="manX"
                     /locus_tag="K0W68_002073"
     CDS             complement(18570..19541)
                     /gene="manX"
                     /locus_tag="K0W68_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBI3073627.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            20004..21554
                     /gene="yoaE"
                     /locus_tag="K0W68_002074"
     CDS             20004..21554
                     /gene="yoaE"
                     /locus_tag="K0W68_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBI3073628.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(21558..23156)
                     /locus_tag="K0W68_002075"
     CDS             complement(21558..23156)
                     /locus_tag="K0W68_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBI3073629.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(23287..24651)
                     /gene="sdaA"
                     /locus_tag="K0W68_002076"
     CDS             complement(23287..24651)
                     /gene="sdaA"
                     /locus_tag="K0W68_002076"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBI3073630.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(24835..25413)
                     /locus_tag="K0W68_002077"
     CDS             complement(24835..25413)
                     /locus_tag="K0W68_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBI3073631.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(25417..26778)
                     /gene="pabB"
                     /locus_tag="K0W68_002078"
     CDS             complement(25417..26778)
                     /gene="pabB"
                     /locus_tag="K0W68_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBI3073632.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26852..27031
                     /locus_tag="K0W68_002079"
     CDS             26852..27031
                     /locus_tag="K0W68_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBI3073633.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(27151..27474)
                     /locus_tag="K0W68_002080"
     CDS             complement(27151..27474)
                     /locus_tag="K0W68_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBI3073634.1"
                     /translation="MVNFKDKNMPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKY
                     LKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEE
                     LKALV"
BASE COUNT         7080 a         6774 c         6943 g         6705 t
ORIGIN      
        1 cataaaacac tatcaataag ttggagtcat tacctggtta acgttcgatc acccggcgat
       61 tcaccgtgta attcgccgtc gtcgatttca aaagcgccta tgcgcacact ggttttcatc
      121 ttcgtgctct ccatttagct gccggttgtg gtacgaccag tccgatcgca ccatttttca
      181 gcctagttga ggcagccgaa tgtgccagtc aaaaagtgcg ctttttttgt gcgctgtcag
      241 gcgttaccgg taaaaagttt cccatcgcgc accagttcac gcggatagct gtttttcaat
      301 cgcgaaccaa tccgtttggc taaaccaatc ggttgatgct ggaaagtaac caacacgtca
      361 tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc ctccgcttcc
      421 tgcggtgtca gttcaaaagc gttcacattg tcgggggtgg caagggcaat aactgcttca
      481 tgctgccagc gataaccttt gttgtgcgtt tcggcaagtt taatccccaa gcgagaaaat
      541 cggactttac cgatcagggc ttcaatgccc accgggaaca accacagttc tttgtcacgc
      601 tgccagaggc gcaggttttc atcccagttt aagccaacac ctgcagccgc ctgacgaatt
      661 tgtccagctt ctcgatcttt cactgggctg aacggaaaat taccgacttt gtatttgggg
      721 gcgggtaagg cgggaatcgc ctgagtttta cgcagacgag caacgaagaa gccttcgcag
      781 tcgtaaattt gtgggaaaac atgcaaaaag ccttcctcgg tcagcgcctt gtttgcacca
      841 gggaagagat caccaagcgg taaaaactct actgcgtcgg ggtaagtctc tttcagccac
      901 aggcaaacgg cttcgttttc ttcctggttt aaggtacagg tcgagtaaac cagcgtaccg
      961 ccagggcgta atgcatgaaa ggcgctgtcg atcagttccc gttgggtagc tgcgatttcc
     1021 tgattgcttt ctggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg
     1081 ccggagcagg gagcgtccag caaaatggca tcaaacattt ctggcactgc cgcaccaaac
     1141 acgcggccat caaaatgtgt gagcgcaaca ttactgatac cacagcggct gatattggca
     1201 tgtaacactt ttacccgact ggcggaaaac tcattggcaa gaattgcccc ttcgttattc
     1261 atccgcgcgg aaatttgcgt cgttttggag ccaggcgcgg cagcgacatc catcacccgc
     1321 tgtggtgcat taccgtcagc aaacaaggcg gcgacgggca acattgaact ggcttcctga
     1381 atataaaaca ggccacttaa atgctcggcg gtactaccca atggcaatgc atcttcattg
     1441 tcgcgttcaa tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa
     1501 ggagcggtta attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg
     1561 cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa aagagagcgt cgaaggcatc
     1621 gcttcgcgca tttgcgtcag aaaggcgtcc gggaaataaa cggtgtgttg ggccacgagc
     1681 atataccact ggcaaaaaaa acaggcgcgc agtttagcat aaacgctccg gcgcaggcac
     1741 gccggagcag tgggcattat ttgggaagcg cagtacccca ttcacgccac tctttcggtt
     1801 cactttcctg caacaggaag tgcttgcctt cctgggcttt cggtgccagt ggcgtacccg
     1861 gaggcgtggc gaaggcgatg ccgccacgga taaactggtt aaaggtgccg gttttcacta
     1921 cgccgcccgt cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt
     1981 tacgcaccag gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca
     2041 atgtccccag cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg
     2101 taccaatacc taacgaaccg gcttccggtg cttcgacaat aatgcttaag ccatccaggt
     2161 aacgcgaatc agtaatagta gcttcttgca actcaaagtc acggcgagga ttaccccgac
     2221 ctggttcgac attgatatac ggctggagga tagtatctag atgctcaacg cccgcggctg
     2281 aaatttgcgg tgtgaccacc gagaagcgcg taccgccgcg ggcgaaggtc tggacatact
     2341 ccggatagag caccgccttt gcctgtactt cattgcgcgc ggtaatcaga tccagcgtct
     2401 ggatttgccc gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg
     2461 catcgaaagc gtgaagcgta atctgcccac caaccgcacg ggccgctgtt tcggaagcat
     2521 acagaattcg tttgtcgcct ttacgctgac tggcgctggc accgctgagg ttatcgaagc
     2581 taatggctcc ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat
     2641 tcagctgaac tttcgccccg ccttctgccc agaacacgct attgctggtc agaaggttgc
     2701 gatactccgg cttaatatgc agatcgatat caaacgcgtt agctcgcgga cgcacggtaa
     2761 tcacttcacc aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca
     2821 gcgtctctgc actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg
     2881 ctttttccag attggcatac agtggatagc tggctttcat ctcaccttta tcgcctggca
     2941 gaatacgtat cccgccgtta atccattctg aggcgctggc accaagaaac tcaacgccat
     3001 ccagccccac cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca
     3061 gttcgcgatg ctgaggctcg atggcgacgg taaaggtaac gcctttgctg gtgagtttac
     3121 gatcaataac ctggcctacc tgcacgccgt gaagaatgag cggctgacca gcatcaatac
     3181 cgtaactttc cggcgcggtc agggtcagcg tcagaacatc aggttcatgc agcagtgctt
     3241 tttcgcctgg cacaacaacg aactctttgc gtggctcgcc atcgccgggt accaactcga
     3301 aggttttgcc agtcagcagg gcgctgagat tggcatcgct aagggataat ttcggattaa
     3361 gtaattcgat gcgggtattt tcacgaagca gggtaacgac gctgggatta actgtcattt
     3421 ccccagtgac tttaccacca ggatttaaat ccagtttagt tagctgtccg acttccagtc
     3481 cctgatacat taatggcgtc gagtcggcgg ttaatccggc cccactcggc agttccagtt
     3541 tgattattac gccacgctgg ctatgggcga ggtcttcata cagaccaaag gtatcttccg
     3601 cctcggcagg tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg
     3661 ccagactttc cagtttcacc ttcgcaccac tgatactgac gttggcatca acgccggaaa
     3721 cgttccagaa acggctacct tttttcacta ggtcggtaaa acgccgctcg atcaggacat
     3781 caatcaccac gccttgcttg ttgggattga tggcatagtc gtagactttt cccacaggga
     3841 tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc gagatcgggg gcttgcaggt
     3901 ggatcatcag atcgccattg tccagtcgat atttcggttg ggtatcgagt gcgacaaagt
     3961 gatcctgctc tttaccttta cctggcatca tgccgatata gttaccaccg acgagggcgt
     4021 ccagcccgga gacacctgcc aacgatgctt ttggcgtcac cagccagaac tgagtctctt
     4081 cgcgcagcgc atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat
     4141 cgtcgctgag gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg
     4201 tacggcccgg gacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt
     4261 cctgataact gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc
     4321 agaaaggtga gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt
     4381 cctgactcat gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc
     4441 aaaattacgg ctgcgccaaa ataaaacgca gccggtccca tagtaaaagc gaggatctga
     4501 tcgcgattaa tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt
     4561 ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta
     4621 aaatgaatgc tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata
     4681 ctggcgataa acacgattcc ggcgaccgca atgttgctac ttgccagcga cataatcccg
     4741 gaaagaatcg tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg
     4801 ttggctggta acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta
     4861 tgacgacgac gcaggcgtag cgggatatgg caacgcgggc agcgaccacg ctgatcagga
     4921 tagccggtaa aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg
     4981 cgctgcggat aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc
     5041 gttaaaatca ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa
     5101 tcctgtacct ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac
     5161 tctttaagac gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca
     5221 aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag
     5281 accatcgccc ccgttatcgt atcgccctgt ttggtcattt gccagatgcc ttgcatgacg
     5341 ttggcgtcga tacgaatacc taacagccag atatgcaaca gcggttcgcc ccaggcaaac
     5401 ggcatcagca acagcatggt gaaagccatt gccgccaggc gcgttagcga ccagtcgcgc
     5461 ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tctgatgaga atttatctcg
     5521 ggcaggctaa acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc
     5581 tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc
     5641 atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt
     5701 gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta agacaaacaa
     5761 gtgattatat aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc
     5821 tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg
     5881 agcgtctcac tgacgtaaac tgggcaggtt tttatttgct tgaggacgat acactggtac
     5941 tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg
     6001 gcactgcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc
     6061 atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc
     6121 agattattgg tgttcttgac atcgatagta ccgtcttcgg tcgctttaca gacgaggacg
     6181 agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca
     6241 aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg
     6301 tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa
     6361 atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg
     6421 aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa atcggtattt
     6481 ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat
     6541 ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa
     6601 cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg
     6661 ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag
     6721 cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcactg cgccgcgaac
     6781 gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc
     6841 aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc
     6901 cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa
     6961 acgcgatgga tgccaccgta ttagaaatca ccaaagatgg cgtccgcgtc cagctgaatt
     7021 cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca
     7081 ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag
     7141 accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg
     7201 cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag
     7261 ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat
     7321 tacagccaca acgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag
     7381 ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg
     7441 caaaagcgcc gttttgagcg ttaccagtac gctttgtcgg tactggaaaa gccgatggat
     7501 ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag
     7561 gctgagttga acgcgctgtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg
     7621 gcaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt
     7681 cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg
     7741 cgtgaaatcg acccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact
     7801 gaaatgagtt tgtcgctgga aggtattggc gcagtgctgc aaatggatga tgactacacc
     7861 gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt
     7921 gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt
     7981 cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt
     8041 ttacctgctg gtaaagggac caagacccgc actgtaacat tgacccgtga acgtattcgt
     8101 ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggcgtg
     8161 ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa
     8221 ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg
     8281 ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc
     8341 cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa
     8401 ggcccgctgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg
     8461 gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc
     8521 gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca
     8581 gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg
     8641 caacgtaaag gcgtaacgcc agacatcatc atgccgacgg gtaatgaaga aacggaaacg
     8701 ggtgagaaat tcgaagataa cgcgctgccg tgggatagca ttgatgccgc gacttatgtg
     8761 aaatcaggcg atttaacggc ctttgggccg gagctgctga aggaacataa tgcgcgtatc
     8821 gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgagg
     8881 gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat
     8941 gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg
     9001 aagaagctgg atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg
     9061 gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaacccgct
     9121 cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag
     9181 atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac ttacgcacta
     9241 tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt
     9301 taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg
     9361 ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc
     9421 aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga
     9481 tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg
     9541 aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggca gggatcgcta
     9601 tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc
     9661 gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag
     9721 ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga
     9781 cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc
     9841 ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc
     9901 tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta
     9961 tcaccatgtg gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg
    10021 ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag
    10081 aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt
    10141 tcatgactca cccgccgctg gataaacgaa ttgaagctct gcgtacgggt gaatacctga
    10201 agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccttg
    10261 gatcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc
    10321 gcagccatca gcgaaacgtg cgtaccatta tcaccgaact gatttagcat cagcgccacc
    10381 agtgccgcgc cgctactctg acccagtaga cgagccgttc ctaacatgcc actggctcca
    10441 ccgctacgtt cgcgaggagc ggaggtaata atggtgtggt tattgggtga ctggaacaag
    10501 ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg
    10561 ggcagcagaa ccaggggaaa aagccccgca gccatgatga acaaccctag agcacccagc
    10621 aatcctgcat gtacgcgttc aatcaaatag cctgccaacg gagccatcac catcgttgct
    10681 aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc
    10741 aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg
    10801 caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga
    10861 agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca
    10921 gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg
    10981 aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa
    11041 ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg
    11101 attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg
    11161 gcagcagaag agacggcaac aatatggcga ttgttggcag ggcgacgttg gcgattgcgc
    11221 cgtcaaggac agccatcgaa atgccaatca caatggttaa tatcgcaccg tatcgctggg
    11281 gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt tggtgaataa
    11341 tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa
    11401 ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa cagttctgat
    11461 ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga taaacagcct
    11521 gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg tgaagagcgc
    11581 gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc
    11641 tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc ggagaaatat
    11701 tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt
    11761 cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac tatccacctc
    11821 ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat gtacaatttg
    11881 cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta
    11941 ctgctggcat ggcgcgatct cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa
    12001 cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct ggaccaggtg
    12061 cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg atgcattgcg
    12121 gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc cttcccgacg
    12181 ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc
    12241 aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta agaacctgtc
    12301 ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg aaggtgcgtt
    12361 atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg gttgtttatt
    12421 acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa tctgttatcc
    12481 ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg gcagtcagat
    12541 aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa acgccatatc
    12601 cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga atgatgaatc
    12661 gcattacaac ctcttctctt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    12721 aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt taactaacgc
    12781 atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg
    12841 gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    12901 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    12961 tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt agaatagcgc
    13021 ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt
    13081 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttatcaatc
    13141 gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    13201 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    13261 aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc ctggtcgccg
    13321 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    13381 tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa
    13441 taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt tcctcattaa
    13501 tttattagga tgtttacatc ggatttgtga ttaagcgtgg tattatttat tagcgaaacg
    13561 tttctctctt gatttttttg ctcattcatc aatttttctt attttaaatt ttcaatccct
    13621 ttggggattg acttctcttt agggtaattg atagccgtta accgactgtt ttatgagaaa
    13681 aagtgatata actttttatt cattgcatag caaaaaatgt gatattgcac gcactatgta
    13741 ataacttctc ccactggcct ggaacaactg aacttattga actatgttag aaaatacgcc
    13801 agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg cgcgtattcc
    13861 gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac
    13921 atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt cgggttaaac
    13981 gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt aaacacgttg
    14041 acgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt aatgtgtcag
    14101 ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt caaatggcaa
    14161 agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc attactccgg
    14221 ctgatggcag caaagacgtg ttcgtacact tctccgctat ccagggtaat ggcttcaaaa
    14281 ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa ggtccggcag
    14341 ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat acgcttcaat
    14401 ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt cgctcctggc
    14461 gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag tatcctgccg
    14521 gagttgtcat gtctttttcc tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca
    14581 gctatatctg tccccagcga catcagtttg atatggcgaa agaagggtat gtcaatctgc
    14641 tgtccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac
    14701 gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt gtcgcccaac
    14761 tgagggaacg gcttgatgat aaggccacgg cggtgctgga tattggctgt ggtgaagggt
    14821 attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggt ctggatgttt
    14881 cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactttt tgtgtcgctt
    14941 ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt atttacgcgc
    15001 cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc attactgcca
    15061 cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa gtacatcttc
    15121 atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg gagttgtgtt
    15181 atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg ccgtttacct
    15241 ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc gactgccaga
    15301 cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc gtccagagga
    15361 tctggacgcc gatgccgatc agtaccagcc cgccgagaat ttctgctttt ttcccaataa
    15421 ttgagccgat aaagcgacca accatcatcc ctaatgttga cataatcaag gttgcacaac
    15481 caatggccaa tgcggtcgcg ataatgttga cctgcaggaa agcaagacca acacccacag
    15541 ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa ccgtgtcgac
    15601 ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc
    15661 cgaggaatat cagcagcaca aacgcaatcc agtggttcca ttcaaggaca aaccggctgg
    15721 ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa
    15781 ggccggttcg caatgcttca gaaaatttcg gtttatggag ggtggcacct ttaccgattg
    15841 atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca gtgatattca
    15901 tgacaatgtc ctgaccgggg tatccataaa acacatcatc gcccccagca aacatgcata
    15961 catgaacaac aagccgtgat ggttttcgcg ttcatgcagg catagcgatt gatgtgcata
    16021 tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa cgagtatgtt
    16081 gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc gcaaccttaa
    16141 catattttga gaatataaaa caactgttgg aatatattat ttcattgctt tattgataac
    16201 gattttcatt tggatttata tatcaatgaa acgttaaaat aaataacaaa taagggaaga
    16261 ggtaatatag cctaagctat gtctgattat taaaaataca ctaaacatag ctttggctaa
    16321 attcataact tattgagttg aaacgagaag cttataaatt ttttccagat cgtcgatatt
    16381 tcgaacgcgg attaacagcc gacgctgttc taattgcatc accaacacgc catcttccga
    16441 caagttcatc gctttgattc ggctatattc aatccagaca ttggcgaaga aaaaaccttt
    16501 ttgtttaaag atgatcttcg gaacgcggat ccagaatata taaaaaccca tcagagccag
    16561 tgcgcttaat aaccatgtcg ttattaacgc accatgattc gtgacgttgt tatagataag
    16621 aatgacaatc aatccgacga agataacgct atcgatgcga ccacgccgga gcaaaggaat
    16681 tgccagcagg gtggggccgt tacggcgggg catgatgaac tgatcgtaga tcgcgaaggc
    16741 caggagtgcg gcgatgaaaa gaatcagtac caggtccgtg attgtcatta atcctccaga
    16801 taaaaaaacg gggccaaaag gccccggtag tgtacaacag tcttacagtc ccagcaggcc
    16861 gcaagcgtaa ccagcgatac cgatgacgaa gaagccaacg atgatccaca gcgggttaac
    16921 ttttttgcgc agtagccaca tacaagcaaa ggtcagcagc agtggtacca ggcctggcat
    16981 taactggtcc agaatagtct ggacagtagt aacgtgttct ttgcccgtct ggtcagtaat
    17041 gcgagagaca accagcggga tgttgacatg tgtccacttg ttaaccaatg cccccatgac
    17101 aaacaggccg aggatagacg ccccttccgt cagtttttgc aggaagccac cacccatatc
    17161 tttaacgata tcgatacctt tggagtaacc atacgctacg ccgtagtaac gggttgccag
    17221 acgcaccagg ttaaacagga tgaagaacag cagcggacct aacaggctgc cgctcatcgc
    17281 gataccggca cccagtgctg caaataccgg acgtacggtt ccccagaaga tcgggtcgcc
    17341 tacaccagcc agcggcccca tcaaaccgac tttgataccg ttgatagcac cgtcgtcgat
    17401 ctctgcgcca ttagcacgct gttcttccag cgccagggtt acgccgagaa tcggcgcagc
    17461 cacgaacggc tgggtgttaa agaactccag gtgacggcga atagcttgtt tacgagcttc
    17521 gttgttctca gggtagaggc gacgaattgc cggtaccata gagaagcaga aacccagtgc
    17581 ctgcatacgt tcgaagttcc atgaaccctg gaagaggtta gaacgcagga agacgccacg
    17641 aatatcactt tgagtgagtt ttttctcggt ggtagtttga gttgtatcaa ccatttcgct
    17701 cacctgttag tccagttcgt tatcgagatc gttgttacca gctgcctgag caggcgcacc
    17761 ggctacgcgg ttgtatttcg ggctaagttg gatgtagagc actgccataa cagtaccaat
    17821 cacacccaga gcaaccaggt taaagttggt gaatgctgcg gttacgaagc cgaggtagaa
    17881 gaacggcatc aggtagccag cacgcatcat gttgataacc atcgcataac caaccaccac
    17941 gatcatgcca ccggcgatat tcagaccatt ggtcaccact tccggaatcg cattcagcat
    18001 gttctgtact tcgctggtac caacagacag cgcaacgatg acggccggaa tagccacacg
    18061 cattgcttgc aggaacagag aagaaacgtg gatccaggaa atcgctgtca ggttgccgtt
    18121 atcagcagcc ttatccgcag cgtgctggaa agcaacggta atagtacgaa cgatgatggt
    18181 cagtacctgg cccgcagcgg ccagagggat tgccagtgcg atacctgcac caatgctctg
    18241 atgacctgcg ataaccagaa tggtagaaat gatagaagcc agagcggcgt caggcgcaac
    18301 tgcagcaccg atgttcatcc agcccagcgc gatcatttcc agcgtaccac cgataataat
    18361 accggttttc atatccccaa gaacgatacc caccagggta cacgcgatta gcggacggtg
    18421 aaactgaaat tcatcgagga ttgatcccat acctgcgata caggctacga taaataccag
    18481 cacaatttga agagtggtaa tctccattgt acttctcctg tttacgtaag acttaagtgt
    18541 gaaaactgag tgataatcaa cacaatacgt tacttatcga ttttgctgat cagatccatc
    18601 attttcagtt tcggatcggt ggaaacctta cggacttcca gctcaatacc gcgcgcattc
    18661 agtttcttga acgcctcgat atctttttca tcaaccgaaa ccgcgttatt cacctgggtt
    18721 ttaccctgac ggaatgccat accaccgacg ttaacagagg tgattttcac gccgccttca
    18781 acgagacgct ctacatctgt tgggttggta aataacagca ttacgcgttc gccagcatat
    18841 ttcgggttgt tgtagacgcg aatcattttg gcaacatcaa ctacgtgtgc tgttacgccc
    18901 ggaggtgcaa cctgggtgag cagtgtctta cgaacggtat ccgcagccac ttcatcacta
    18961 acaacaataa tacgggagac attggtttct ttggtccagc gggtggcgac ctgaccgtga
    19021 atcagacggt cgtcgatacg cgcaaggcca ataaccatgt agtcgtttgg ccccattggt
    19081 tttgccggag ttggagccgc ttttggtgct gcggcagcgg gtgctggcgc ggctttttca
    19141 accggtttgg ctttcagtgc tttcacgcct tcacggcctg tttctactgc cagcgcaacc
    19201 agttcatcaa agcttgggtc atcatcacga gccattaacg tttccacgag cattggaatg
    19261 ttaacgcctg caatgacttc ataatgctct ttgtcgacga caatgcggct ggcagcattg
    19321 aacgggctgc ctccccatgt atcaacgaga aacagcacgc ctttagtggt gtcgagtttt
    19381 gccaactgag cgttgtactt ttcaatcagc gtttcggcat tttcacctgg aacgaaatcg
    19441 atccagccga cgttttcctg ctcgcctaac agcatttctg ccgttttaag caactgctct
    19501 gcagcccaac catgtgtgcc tataacaata gcaatggtca cttgctacct cctttattat
    19561 cgttaacacc tcaacgtgcc agatgtattt ttgaatcgtc gtccacaatc gaatcgattc
    19621 agataagggc tacaaccaaa aacccctgcg tttcgcgatt tattttagat atcgaaaaaa
    19681 ttattttatg tgatgaagat ccgtaattta actttcgatt agcagaaatt tcgaaaggta
    19741 aaatatcctt gtcacattcg tttgcaaagg aaggtaaatc tttgccaaat cagaggcgtc
    19801 tctgatatgt ttaactcccg tttaacaacc atggagtata gggcagtagc ccgcagtatg
    19861 gatcgtcacc gacgtcattt cagcatcagg ccttttaacg cctgcctttc tggcactcta
    19921 tgccgcacct ttcgtttgca ttttgtcgtt acgcctgcat tatttctggc gtcgaatagc
    19981 tattccttaa gcaggagctt gtcatggaat tcttaatgga cccctcaatt tgggcggggc
    20041 tactcacgct tgttgttctc gaaattgtgc tgggtatcga taacctggtc ttcatcgcca
    20101 ttcttgctga caaactgccg ccaaaacaac gcgataaagc gcgtttgctg gggttatcac
    20161 tggcgctgat tatgcgtctg gggctgctgt cgctgatttc atggatggtc acgctgacca
    20221 aaccgctatt taccgtcatg gatttctcct tctccggacg cgacctgatt atgttgttcg
    20281 gggggatatt cttgctgttc aaagcaacaa ccgaactgca tgaacggctg gaaaaccgcg
    20341 atcatgattc cggccacggt aaaggctacg ccagtttctg ggtggtcgtc acacagattg
    20401 tcatccttga cgccgtcttc tcgttggatg cggtaattac tgcagtaggg atggttaacc
    20461 atctgccggt gatgatggcg gcggtagtga ttgcgatggc ggttatgttg ctggcatcca
    20521 aaccgctgac gcgattcgtt aaccagcacc ccacggtggt ggtgctctgt ctgagcttcc
    20581 tgttaatgat tggtctgagt ctggtggcag aaggtttcgg tttccacatt ccgaaaggtt
    20641 acctgtatgc cgcgattggc ttctcgatca tcatcgaagt gtttaaccag attgcgcgtc
    20701 gcaactttat tcgccaccag tcgactttgc cgctgcgagc gcgtactgcc gatgccatcc
    20761 tgcgtttgat gggcgggaaa cgtcaggcca atgttcagca cgatgccgat aacccgatgc
    20821 cgatcccgga aggtgcattt gccgaagaag aacgttacat gattaacggc gtactgacgc
    20881 ttgcgtcgcg ttctctgcgc gggatcatga cgccgcgcgg tgaaataagc tgggttgacg
    20941 ctaatctcgg ggtcgatgaa atccgcgagc aactgctctc ttcaccgcac agtctgttcc
    21001 cggtatgtcg cggtgaactg gatgaaatca tcggtattgt acgtgctaaa gaactgctgg
    21061 tggcgctgga agagggcgtt gatgtggcgg cgattgcttc ggcgtctccg gcgattatcg
    21121 tcccggaaac cctcgatccg atcaacctgt tgggcgtgct gcgtcgtgct cgcgggagct
    21181 ttgttatcgt gaccaacgag tttggtgtgg tacaaggtct ggtcacgccg ctggatgtgc
    21241 tggaagccat tgcgggtgaa ttcccggacg ctgacgaaac gccggaaatc attactgatg
    21301 gtgacggctg gctggtaaaa ggcggtacag atttgcatgc cttgcagcag gcgcttgatg
    21361 ttgagcacct tgccgatgac gatgatatcg cgacggtcgc gggcctcgtg atctcggcaa
    21421 atggtcacat tccccgtgtg ggcgatgtga ttgatgtagg gccactgcat atcaccatca
    21481 ttgaagccaa tgattatcgt gttgatctgg ttcgcattgt taaagagcaa ccggcgcacg
    21541 atgaagatga gtaagcatta acgtaacggc ataatgggcg tgatatgtcc attatgccgg
    21601 gcgggcggcg gttggctgcc cgccagccat ttgggaaaat cacgtagcgg catcggctgt
    21661 gcgtacaaat acccttgcaa aatatgcacg ccatgacggc gcaaatactt cgcctgttct
    21721 tgcgtttcca cgccctccgc caccagttca atattcagtc tttgccccag cgcgatgatg
    21781 atatcggtca ccgtcgagtt aaccgcgtca gaacctatag ctgcggtaaa tgacttatca
    21841 attttcagca tgtcaggacg taatgtttca agccaggaaa atgaactgtt gccggtgccg
    21901 aagtcatcaa tcgccagttt gacgttttta cgatgtagct cgcgggcaat ccgataatca
    21961 acatccagta aggcatcgcg ttcggtgatt tcgaggatca gttgctgaat cgggtgagcg
    22021 ctaaaccagt actgattgag atcttttatc agcacaccac ggcgaaaatg gctgggtgcg
    22081 acgttaatgc caacatgaaa ctgactactc atcgggaaaa catggcgctg acgaatggtt
    22141 tctgccatca catagcgggt cagtggcaca attaaatgat gttcttccgc gataggaata
    22201 aacacatccg gtgaaatcca gccctgacgc ggattgttcc agcgcagcaa aatctctaca
    22261 ccaatacatt gctggctgcg cgcattaagc aaaggctgac agaacaattc gaactcatgt
    22321 tgcgccaggc ccagattgat ttcgcgggaa aagctcatcc ggtaagcggt cgccagccag
    22381 gcgatgtagc ccaccagtaa acttagcagc accgccagcg gtaattgtgt tggaagatag
    22441 tgccatgcca gcgccgtagc ccccggacca ttaacgttaa tggtaaaagg aaagcgttgc
    22501 gaagaaacct gatagttttc attgtcttca ggttgcggaa gggaatctac cagcccatta
    22561 ccatagagca aatgccgttt gtccaccgtc aggctggcgc tactgatttg cggcagttgt
    22621 ggctcaagga gcattgccgt cagtaagtcg atgttaatca tctccatgac cccagcattg
    22681 ctactgcccg ctgcaggcgt ccattgaatc aaaaccggac tgcctttaat cagggcacgg
    22741 tcgatcgtca ggcgtaaaag tggttgcggt gcaggaagtt cagccagaat gtccacgacg
    22801 ggcacattgc gataaccaaa aatgctggag caatagagtg ttccgtcttg caccaggcca
    22861 atggatcgca cagtttggag ttttgccgcc tgtttacgta atggcaaatg ggcgacagag
    22921 cagggcagac caatcagcgg aagcaagact tcgctacctg cctgtagggg aagcagtact
    22981 ttatctaatt cctctacagc gtgattggcg aattgtacta cccgttgttg atttaagtta
    23041 cgctgtgaaa taaatcgcac gctcagggtc gaagcgagcg taacgagtgc gcaaatcgta
    23101 caaacaatca ttcgattacg gcgataggtt ttaatgatcc gttgtgcttt ttgcatgcgg
    23161 gccaccctgt aagcgtatgg cgacaaacgc ctgacgcaac agtggaagtg tagtggggaa
    23221 tattacagca gacgagaaag cgggtataaa ttcgcccatc cgttgcagat gggcgagtaa
    23281 gaagtattag tcacactgga ctttgattgc cagaccaccg cgtgaggttt cgcggtattt
    23341 ggcgttcatg tccttaccag tttcgtacat cgtttcgatg accttatcca gcgagacgcg
    23401 cggtgcactg gtgcggcgca gagccatccg tgcggcgtta atcgccttca cagaggcaat
    23461 ggcattacgc tcaatgcacg gcacctgaac ctgccctgca accgggtcgc aggttaaacc
    23521 aaggttgtgt tccatgccaa tttccgccgc cacgcaaacc tgttccgggc taccgcccag
    23581 cagttctgca agacccgcag cagccattga acaggcaaca cccacttcgc cctggcaacc
    23641 aacttccgca ccggaaatag aggcgttcat tttatacaat gcaccaatcg cgcccgctgc
    23701 cataaagtaa cgggtataga tgtccgggct gaccgattca ataaagtggt catagtaagc
    23761 cagcactgcc ggaacgatac cgcaggcacc gttggttggc gcagttacca cacgaccacc
    23821 ggcggcgttt tcttcgttaa ctgccagcgc aaacatgttt acccagtcaa tgacattcat
    23881 cggatcgtta gacagtttat cgctggaaac taacatccgg cgcagggcag acgcacgacg
    23941 tggcacgcgc agcgggcctg gcagtacacc ttcggtgttc atcccgcgat cgatacatgc
    24001 ctgcatggtt tgccagacat gcgcgaaata ctcgtcgatc tctttcttgc tgtgcagcgc
    24061 cagttcgttc tgcatagcga gaccagacag cgaatagccg gtttcattac agtacgcgag
    24121 cagttcggtg gcagatttga acggatacgg cacgctcact tcgttggcag catcctgacc
    24181 aaagtgttct tcatcgacga taaaaccgcc gccgatggaa taataagttt tgctgtagac
    24241 gacttcatcg ccgttatagg cgtggatttg cataccgttt tcatgcagcg gcaggttgcc
    24301 gttatgaaaa cgcatcccgt tgtcgcgcgg gaaatccact tcatgccgtc cctgtgccag
    24361 cagcagacgt tcgcgctctt ctacgtcgcg aataaaaccg ggaatactgt cgatatccac
    24421 ggtggcaggt tcgttacctg caagacccat aataatggcg atatcggtgt ggtggccttt
    24481 acccgtcagc gacagtgaac cataaacgtc cacggcaacg cgagtaacgc tatccagtaa
    24541 gcctttttcg accagatcat cgacgaactg tttacctgcc ttcataggcc ctacggtatg
    24601 ggaagatgag ggaccaatcc ccaccttaaa catgtcgaat agactaatca cgataatact
    24661 cctgacaagg tgactggact tccagtaacg aactaataac agcgcatagt gtaagaggga
    24721 acgcggcgac tggcttaact attcacatga attaaactaa tcaaaaaccg cgatttaact
    24781 aatattgcga tgattgtctc aagtagtgtt ttgtagatgt aaagataagt atagtcaggg
    24841 tttcacacca atttgcagcg ccagctcacg aattatgcct gcggtcattc cccatacaaa
    24901 atactgttcg taccaggaca gccatacccg atgtgaatcg ccacggcggt agatatctaa
    24961 agggtgataa cgacccagat gtaatgcctg ggcgagcggc atttcaaaca ccgccgagac
    25021 ttcatcttca ctggcgcgat acggcagatc gggcgggata atgccgacca ctggggttac
    25081 ctgatagcca gttacgctat cgacgggcgg cagcacgccg ataacttcaa cggcggaagg
    25141 cggtatagcg acctcttctt cagcttcgcg cagcgcggcg gcgataactg atgcgtccgt
    25201 gtcatcgact gcacctccag ggaatgccac ttgtccagcg tgtttacgca gatgaattga
    25261 acgctgagtc agcaacaacc ccggttgcgg tcgacggacg atggggatta acacggcagc
    25321 ctgacgatga tttagggttt cccggttaat ttgcgggcgc aaaagttgaa agcgcgataa
    25381 aaaatcatca agcatcaggc tacggtattc cacgtcttac ttctccagtt ggcgtaatat
    25441 cttattaact ttatcaaaag tttcctgata ttccgcttct tcctggctat cggcgacaat
    25501 tccaccgccc gcagagcagt atatttgtcc gttaatggca gtcagagtgc ggatagtaat
    25561 gctggtatcc atgttgccgc aaaagctcaa atagccaatg ctgccgcacc aggcattacg
    25621 tcgctgcggt tccagttcgt cgataatttc catagcccgt actttcggag ccccggttat
    25681 tgagccacca ggaaaagctg cgcgcagcag atcgctggcg tgtaactgtt ctggtagttg
    25741 tgccgttatg gtgctgacca gatgatgcac ggcagggaag ggttccacca cgaacagctc
    25801 tggtactttt accgaacctg ctacggcaac acgaccgata tcattacgca ttaaatcgac
    25861 aatcatcaga ttttcggcac gatctttcgc tgagttcgcc agtttttctg cttgtttgct
    25921 atcttcctga ggatcgggca ggcgtggtag cgtgccttta atagggcggg tctggatttc
    25981 actattatca caaagaataa accgctctgg cgaaaggctt aaaattgcac cctgttcaag
    26041 acgtaaaaaa gcgctaaatg gcgcgcggtt ggcctgatta agctgaagga atgcctgcca
    26101 ttcatcgcca gaataggtcg catgaaaacg ctgggcgaga tttacctgat agcaatcacc
    26161 gctgtgcaga tattcctgta cctggcgaaa tttttcgccg tactgctcgc gggtcatatt
    26221 ggattgccag tcggaagtaa gcgtgaaatc ttcctgcggc gagaattgct ggctttccag
    26281 ccaggcccga cgggcattga catcattatg actcagcaaa gaaactgtat gacgctggtg
    26341 gtcgacaatg agcgcccaat cgtagatacc cactgccata tccggcagaa cgatatcttg
    26401 ttccgcaatt tctggcagtg actcaaaacg gcggcccaga tcgtagccaa acaaccccag
    26461 tgcgccgccc tgaaatggca aatcttcgtt atgcgttggg cgaatgtctg cgcgatccag
    26521 cacctgctgg agcacctgta gcgggtcatc agtggtcgtt gtgcgttttt cgctttcact
    26581 aacaacggtt tctttaccta aagtggttaa agtgcaaatc ggatcggcga ccacaatatc
    26641 aaagcggcta tacggatgat cggcatagcc ggagtgtaaa agcatcgccc acggcaggtg
    26701 gcttaagcgg gagaaataaa attcagcggc gtcctgacgc cagggtaaag taatcacagc
    26761 gggagataac gtcttcatca gtcctgactc tactggctat gtgccgggta gcatgaaata
    26821 attagcgcca acaatttagc aggagttaac aatgtttgca ggtttacctt cactcaccca
    26881 tgaacagcag caaaaagctg tcgagcggat ccaggaactg atggcacaag ggatgagcag
    26941 cggccaggca atcgcgttgg tggcagaaga gctgcgtgcc aaccattccg gcgagcgaat
    27001 cgtggcgcgt tttgaggatg aagacgagta acacactaat ccgtattgat ttcgttatac
    27061 aagccgatga ttatttgatt gtgagaaaaa ggacgctttt tatatgtgat taaaagcgtc
    27121 ctgatccatt tatgctgatg taaattttac tcagaccagt gcttttagtt cttcctgcat
    27181 atatgttgaa aagtattcag gatttttaat cacagagcgt tcacctgact ccattttttt
    27241 tgcccatcca accatttttt ccgtaaaccc agggttccag ccttcctggt cagctcgcgc
    27301 cgtaatatca gcggcactcg caggtactcc cagttctttt aaatatttaa agattccctt
    27361 cgccgtgctt tcattcatcg aacttggtgt tggcgctgat acatccatgg caccaatgaa
    27421 atccagggct ttatctatta cagcaggcat attcttatcc ttaaaattaa ccatgttatc
    27481 gataaggcta tatatgacct ta
//
LOCUS       DAETQN010000049        27102 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.049, whole genome shotgun sequence.
ACCESSION   DAETQN010000049 DAETQN010000000
VERSION     DAETQN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27102
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.049"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..921
                     /locus_tag="K0W68_002081"
     CDS             <1..921
                     /locus_tag="K0W68_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3073635.1"
                     /translation="SATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLN
                     DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVN
                     IGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLT
                     APQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQM
                     RIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTV
                     NMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="K0W68_002082"
     CDS             complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="K0W68_002082"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBI3073636.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1888..3924
                     /gene="lpoA"
                     /locus_tag="K0W68_002083"
     CDS             1888..3924
                     /gene="lpoA"
                     /locus_tag="K0W68_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBI3073637.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3882..4277
                     /locus_tag="K0W68_002084"
     CDS             3882..4277
                     /locus_tag="K0W68_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBI3073638.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4297..4887
                     /gene="diaA"
                     /locus_tag="K0W68_002085"
     CDS             4297..4887
                     /gene="diaA"
                     /locus_tag="K0W68_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBI3073639.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4897..5472
                     /gene="yraP"
                     /locus_tag="K0W68_002086"
     CDS             4897..5472
                     /gene="yraP"
                     /locus_tag="K0W68_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBI3073640.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5586..6626)
                     /locus_tag="K0W68_002087"
     CDS             complement(5586..6626)
                     /locus_tag="K0W68_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBI3073641.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6699..7334)
                     /locus_tag="K0W68_002088"
     CDS             complement(6699..7334)
                     /locus_tag="K0W68_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBI3073642.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7462..7980
                     /gene="yhbO"
                     /locus_tag="K0W68_002089"
     CDS             7462..7980
                     /gene="yhbO"
                     /locus_tag="K0W68_002089"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBI3073643.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7960..8403)
                     /locus_tag="K0W68_002090"
     CDS             complement(7960..8403)
                     /locus_tag="K0W68_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBI3073644.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8454..8756
                     /gene="yhbQ"
                     /locus_tag="K0W68_002091"
     CDS             8454..8756
                     /gene="yhbQ"
                     /locus_tag="K0W68_002091"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBI3073645.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8743..9246)
                     /locus_tag="K0W68_002092"
     CDS             complement(8743..9246)
                     /locus_tag="K0W68_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI3073646.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9240..9764)
                     /locus_tag="K0W68_002093"
     CDS             complement(9240..9764)
                     /locus_tag="K0W68_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBI3073647.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9973..10968
                     /locus_tag="K0W68_002094"
     CDS             9973..10968
                     /locus_tag="K0W68_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBI3073648.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10977..11855
                     /locus_tag="K0W68_002095"
     CDS             10977..11855
                     /locus_tag="K0W68_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBI3073649.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12061..13068
                     /locus_tag="K0W68_002096"
     CDS             12061..13068
                     /locus_tag="K0W68_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HBI3073650.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="K0W68_002097"
     CDS             complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="K0W68_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBI3073651.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="K0W68_002098"
     CDS             complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="K0W68_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBI3073652.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="K0W68_002099"
     CDS             complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="K0W68_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBI3073653.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="K0W68_002100"
     CDS             complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="K0W68_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBI3073654.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="K0W68_002101"
     CDS             complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="K0W68_002101"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBI3073655.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="K0W68_002102"
     CDS             complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="K0W68_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBI3073656.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20446..21390)
                     /gene="truB"
                     /locus_tag="K0W68_002103"
     CDS             complement(20446..21390)
                     /gene="truB"
                     /locus_tag="K0W68_002103"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBI3073657.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="K0W68_002104"
     CDS             complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="K0W68_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBI3073658.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21955..24627)
                     /gene="infB"
                     /locus_tag="K0W68_002105"
     CDS             complement(21955..24627)
                     /gene="infB"
                     /locus_tag="K0W68_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBI3073659.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="K0W68_002106"
     CDS             complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="K0W68_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBI3073660.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="K0W68_002107"
     CDS             complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="K0W68_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBI3073661.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26826..26902)
                     /locus_tag="K0W68_002108"
     tRNA            complement(26826..26902)
                     /locus_tag="K0W68_002108"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26866..26868),aa:Met,seq:cat)
BASE COUNT         6419 a         7516 c         6811 g         6356 t
ORIGIN      
        1 tctgcaacgc taagtagcac taactggcaa tacgcctgtt cctgctctgc gggtaaggca
       61 gttaaacttg tctatatggt cagccccgta cttaccacca ctggacatca gacaggatat
      121 tacaaactca atgacagcct ggatattaaa accacattac aggcaaacga cattccagga
      181 ctcacaaccg accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc
      241 gtatattctg ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc
      301 gttaatattg gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg
      361 atgaccattc cgaaaacgga tattgccgtc attaaaggcg cgtgggtcga tggaatggga
      421 agcccgtcta caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc
      481 gccccacagt cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc
      541 aatggtcaga agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt
      601 gatatcactt atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc
      661 gacggtacaa ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac
      721 aatgtgcccg atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct
      781 tttcagaacg gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc
      841 tggccagtta atctggtcgg tggtgagctg gaaacaggaa aatttcaggg caccgccacc
      901 attaccgtca tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca
      961 ctgttacccc tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat
     1021 ttctgcggcc agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt
     1081 gcgcagggca tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac
     1141 catttcgcct ttgcgacggt tttcatcttc ctttacccac gccagcagct cgccaacggg
     1201 cgcgccgtga atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga
     1261 ttcgcctaat accgcaacga tatcttccag gctatctaac agacggtggg tagattcata
     1321 aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga
     1381 tttggcaggt aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa
     1441 cgcagtgata gcagcacacg gtccgggtag cggcaccaca cgaatccccg cttcacggca
     1501 ggtacgcacc agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag
     1561 cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt
     1621 atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt
     1681 gtgacgagta tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg
     1741 ggtgatatcc gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt
     1801 atccgccgat tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt
     1861 gcgtaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc
     1921 gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact
     1981 cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt
     2041 cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt
     2101 gcactggtga aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa
     2161 gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg
     2221 cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa
     2281 caaaaccagc aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc
     2341 attgatttac tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag
     2401 cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg
     2461 ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg
     2521 tttgataacc gtaacgatcc cgacatgatg aaagccggga tcgccgactg gcaaaaacgt
     2581 tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt
     2641 aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta
     2701 tttggtcgca ccattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg
     2761 gcggctcagg tagctgccgc acctgccgca gacgtagctg aacaacctca gccgcaaacc
     2821 gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgatcagcct
     2881 gcggcccagc cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc
     2941 gcaaatccat ccgcagagct gaaaatctac gatacctcat cacaaccact tagccagatc
     3001 ttaagccagg ttcagcagga tggcgcgagt attgtggttg gtccgttgct gaaaaataac
     3061 gttgaagagt tgctgaagag caacaccccg ctgaacgtgc tggcactgaa ccagccagag
     3121 aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt cacctgaaga cgaagcgcgc
     3181 gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc
     3241 agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa actgggcggc
     3301 ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc
     3361 ggttctggta tcgctttaac gggtaccccg attactccca gagcgacaac cgactccggc
     3421 atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat
     3481 ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa
     3541 ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa tgctgtacgc cagctcccgc
     3601 agcgcacaag gcaccgcagg cccggatttc cgtctggaga tggaaggttt gcagtacagc
     3661 gaaatcccga tgctggcggg cggtaatctg ccgttaatgc agcaggcact cagcgcggtg
     3721 aataacgatt attcactggc tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca
     3781 aatcatttct cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc
     3841 ctgacggcaa acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa
     3901 ggtcaggtag tccccgtcag ttaaccacca aacagaccgg cgatgcgtgg gaagcacaag
     3961 cgcgtcgctg gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg aacgagcgtg
     4021 gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct
     4081 atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca
     4141 aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg
     4201 attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct
     4261 ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc
     4321 ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc
     4381 cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt
     4441 aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa
     4501 acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg
     4561 attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat
     4621 gcgggagatg tattgttagc catttccacc cgtggcaaca gccgcgatat tgttaaagca
     4681 gttgaagccg ccgttacgcg tgatatgacc attgtggcat tgaccggcta tgacggcggc
     4741 gagcttgcag gtttgttagg gccacaggat gtggagatcc gcattccttc gcatcgtagt
     4801 gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac
     4861 acgcttttcc ctcaccagga tgattaagga gaatacatga aggcattatc gccaatcgca
     4921 gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct
     4981 gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt
     5041 accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaaacc
     5101 cgtattaatg tcacggcgta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct
     5161 gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat
     5221 aacgagattc gtcagggcca gccgattggt ctgggcgaag catctaacga tacgtggatc
     5281 accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa
     5341 gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa
     5401 gcggcggcag atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg
     5461 tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     5521 tacaacgatg caaaccgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc
     5581 tcaaatcaga ataacagcgc cagcccgccg acaatcacgc ctgacactgc caccatcgcc
     5641 cccgtcagcc ataaggcttt cgccgggaac gctttgcgca gcataatcag tgacggcaaa
     5701 ctcactgccg ggagcgtcat caacaatgcc agcgccggag cggttcccat acctgccagc
     5761 atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct
     5821 accgccatcg ccaccaccca catcaggctg ttatcgacag caccatcggc atgggggaat
     5881 aaccagacgc gtgcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc
     5941 cagaaaagtg tccatagcgc cctgccccag cggctaaaaa aaccgctctg tgcttccggt
     6001 atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc
     6061 gtcgcaatca gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag
     6121 ccgaggacaa accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag
     6181 aatgccagcg caccgcccat cgatacctgt tggcgacgca ttcccgccgc gaccggagcc
     6241 gcacagcagg tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga
     6301 aagcgcgatt gtccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac
     6361 gaaccgagga tcacccccag caccgccgct ttccataccg cgaggaaata gatcatcgcg
     6421 taatccaacg ccgcctgcca cggattagca tccgcctgcg caaggataga tttaccgata
     6481 ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag gttgccattt cacataccag
     6541 agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc
     6601 gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc
     6661 gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat
     6721 tctgaagagc ttaaaatagt cacgccttca tgctcaggcc gcatcgcctc tgccagcatg
     6781 acccgcgcca cgtctctcgc atcaatggat ttccagttac cgggcagtaa acgaaacagc
     6841 ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cgcccagcaa catcgacggg
     6901 cgagcaatgg tcaatttcgg ccagttctgg gcaattaatg cttcttccat ctcccctttg
     6961 acgcggttat agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata
     7021 tgctgcgcgc ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca
     7081 tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca
     7141 atgtcgatgg gttcggtgac ctgcgccaac gcgtcactca gttgcggatc atggggatta
     7201 aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cggctatggc gttaactttc
     7261 ggttcgttaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc
     7321 agtacctgac tcatcttcgc tcctttacag aattgtctgc cttgcgctcc acggctcatg
     7381 caccaggctt aatagaccat gaggtaatta tccccgattg tgtgaaattc gcctcatcca
     7441 atgcaacaac gcggaggaag catgagtaag aaaattgccg ttttaatcag tgatgaattt
     7501 gaggattcag aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt
     7561 accattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc
     7621 atcgataaat ccatcgatga agtgacgcca gcggagtttg atgccctgct gctaccgggt
     7681 ggtcattcac cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt
     7741 gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc
     7801 gatgtgattc gggggcgcaa actgaccgca gttaaaccga tcattattga tgttaaaaat
     7861 gcgggcgcgg aattttacga tcaggaagtc gtggttgata aagatcagct ggttaccagc
     7921 cggacaccgg acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga
     7981 gtcgcgtagc caaatcattt ttttaccaaa gcccagcgtg ttgtcggtga atttgatttc
     8041 atcgagccgg atttcccaca ccggggccga cagcattctg gcaaccggaa agcgacgatt
     8101 gtacgcctgg cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa
     8161 ctgcacgccg cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc
     8221 agcctgcggc ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc
     8281 gactttctgc gcatcaaaca gataaaaagc attagcgcac cacagttccc cttcctgctg
     8341 cacacaccag gtgacaacat gttgttttgc caaccaacgg ctgatggtga tgagtgtttc
     8401 cattgctgct ctcttttata ctgtgggcct gaccttaaca tgcgacgcag acgatgacac
     8461 cctggtttct ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg
     8521 atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga
     8581 aaggagaact gacgctggcg ttttctgcgc cagtgggcga tcgttcgctg gcgttacggg
     8641 cggaatatcg cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg
     8701 cagggtttgc agagctgtta agcagtctgc aaaccccgga gattaaaagc gattgaaatg
     8761 ctcgtgatac tcaaccagac cggttacgcc attcagcgcg tcatccgcta aacgatgtac
     8821 ctggaaggcg ctttcagtgc ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc
     8881 aaagccgaaa cgactgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa
     8941 ctcattaagc gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta
     9001 tttttcatca accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc
     9061 aaccggacta aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc
     9121 cagcgtcaga aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc
     9181 gaatgagcga cgcagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat
     9241 tagcatggtt cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca
     9301 acaaaatccg ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca
     9361 atggcgtcca tcaggttttt cacatacagc cccagctccg tatcgccttc aatcaccagc
     9421 cgacgctgga agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg
     9481 ctggcgtcgg cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca
     9541 ttcaccaccg aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg
     9601 ccttcaagaa actccagctc accatcatcc agcgcctgac ggaattgcca gcttaagacc
     9661 tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga
     9721 gatggcccca aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt
     9781 ttcaacaatc atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat
     9841 tccatatgaa attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc
     9901 gcagcaaggt taactaaaat cccagttcgt taacattttt gcgttttgaa agcgcaacct
     9961 tcaggaaaaa ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc
    10021 atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag atgatactaa tgcccgtcac
    10081 ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa
    10141 catcgccgca aacttcacat cgcgattaac acttttgcgc atccggacgg ttacgcccgt
    10201 tggcagcgcg ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac
    10261 ctcgccatgc tggagtacgc cgccgagcgt tacccgcata ttgagcgtca tgtgtcagtg
    10321 caggcttcgg cgaccaatga agaggcgatt aacttttatc atcgccattt tgatgttgcc
    10381 cgcgtggtgc tgccgcgcgt gttgtcgatt catcaggtga aacagctggc acgggtcaca
    10441 cctgtcccgc tggaagtctt tgcttttggc agcctgtgca ttatgtcgga aggtcgttgc
    10501 tatctgtcgt cgtatctgac gggtgagtcg cccaacaccg tgggcgcgtg ttctccggcc
    10561 cgtttcgtgc gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg
    10621 atcgaccgtt atcaggatgg cgaaaacgca ggttatccga cgctgtgtaa agggcgttat
    10681 ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa
    10741 ctgctgccgg agttaatggc ggcgaatatt gcttcggtga aaattgaagg ccgccagcgt
    10801 agcccggcgt atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag
    10861 gccgatccgc aaaacttcgt accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc
    10921 gaaggcacgc agaccaccct tggcgcgtat caccgtaaat ggcagtgaga agagcaatga
    10981 aatattcctt agggccagtg ctgtggtact ggccaaaaga ggcgctggaa gaattttatc
    11041 agcaggccgc caccagcagc gccgacgtga tttatcttgg tgaagcggta tgcagcaagc
    11101 gccgggcaac caaagttggc gactggctgg agatggcaaa atcgctcgcc ggaagcggga
    11161 agcagatagt gctctccacg ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga
    11221 aacgctatgt tgagaacggt gagtttctga ttgaagccag cgatctcggc gtggtgaata
    11281 tgtgcgctga acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg
    11341 tgacgctgaa aatattgctc aaacagggca tgatgcgctg gtgtatgccg gtggagcttt
    11401 cccgcgactg gctggtgaat ctgcttaatc agtgcgatga gttggggatt cgcaaccagt
    11461 ttgaagtgga agtcctgagc tacggtcatc taccgctggc ctactccgcc cgctgcttta
    11521 ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga
    11581 acgggcgcaa cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat ggcattcaga
    11641 ccatgagcgg ctacgtttac aacctcggta acgagctgac atccatgcag ggattggttg
    11701 atgtggtccg cctgtcaccg cagggtactg acactttcgc gatgctcgac gccttccgcg
    11761 ctaatgaaaa tggcgctgcg ccactgccgt tgacggcaaa cagcgactgt aacggctact
    11821 ggcggcggct ggctgggctg gagctgcaag cctaagtaaa tatgctcgtc atacttcaag
    11881 ttgcatgtgc tgcatctgca ttcgttcacc ccagtcactt acttatgtaa gctcctgggg
    11941 attcactctc ttgtcgcctt cctgcaactc gaattattta gagcacagct cactttgtta
    12001 acaactttaa ctactcttta atgcagtatt aaagattaat cggtaacaaa gtgagctgtt
    12061 atgactgata aaaccattgc gttttcgcta ctcgatctgg cccccattcc cgaaggttct
    12121 tcagcgcgag aagcattctc ccactctctc gatctcgccc gtctggctga aaagcgcggc
    12181 tatcatcgct actggctggc agaacaccac aatatgactg gcattgccag tgctgccacg
    12241 tcggtattga ttggctatct ggcggcgaat accaccacgc tgcatctggg gtctggcggc
    12301 gtgatgttgc ctaaccactc accgttggtc attgccgaac agttcggcac gctcaataca
    12361 ctctatccgg ggcgaatcga tttggggctg ggtcgtgcgc caggtagtga ccaacggaca
    12421 atgatggcgc tgcgtcgcca tatgagcggc gatattgata atttcccccg cgatgtcgcg
    12481 gagctggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc
    12541 tatggcgaga aaatccccgt gtggttgtta ggctccagcc tttacagcgc gcaactggcg
    12601 gcgcagcttg gtctgccgtt cgcgtttgcc tcacacttcg cgccggatat gttgttccag
    12661 gcgctgcatc tttatcgcag caacttcaaa ccgtcggcac gactggaaaa accatacgcg
    12721 atggtgtgca tcaatattat cgccgccgac agcaaccgcg acgctgaatt tctatttacc
    12781 tcaatgcagc aagcctttgt gaagctgcgc cgtggcgaaa ccggacaact gccgccgccg
    12841 attcaaaata tggatcagtt ctggtcaccg tctgagcagt atggcgtgca gcaggcgctg
    12901 agtatgtcgt tggtgggtga taaagcgaaa gtgcgtcacg gcttgcagtc gatcctgcgc
    12961 gaaaccgacg ccgatgaaat tatggttaac gggcagattt tcgaccacca ggcgcgcctg
    13021 cattcgtttg aactggcgat ggatgtgaag gaagagttgt tgggatagtg tgtcttaacg
    13081 cgggaagcct tatccgagct ggcaacgctg tcctacgtag acctgataag cgaagcgcat
    13141 caggcattgt gtaggcagca gaaatgtcgg ataaggcacc gctgattact gatacaccgg
    13201 cagcaaatta aagctcgata agatatggac cagtgcgttg ccaacgccaa acaccagaat
    13261 cagcgtaatc atcggcttgc cgccccagac acggaatttc gggctgccaa agcgtttacg
    13321 cgatgcgcgg gctaacagcg ccggaacaat tgccgcccag atggtagctg ctaaaccagc
    13381 ataaccaatg gcgtacagga atccgttcgg gaacagtaga ccacccacaa caggcggggc
    13441 aaaggtcagc aatgccgttt tcaagcgccc cagggctgag tcatcgaaac caaacagatc
    13501 tgccagatag tcaaacaaac ccagcgttac gccaaggaac gaactcgcta ccgcaaagtt
    13561 tgagaacacg accagcagaa ggtccaggct acggctgttc agcacgccgc ttaatgcctg
    13621 taccagcaca tcaatattac cgcccttctc tgcaataccg ataaactccg gacgcgggat
    13681 gttacccatc gtcgccagca accagatggt atacagcgcc agtgccatca gcgtaccgta
    13741 caccaggcat ttcacgatgg ttttcggatc tttgccgtaa tacttcatca ggctcggcac
    13801 gttaccgtga taaccaaacg atgccagaca gaacggcagg gtcatcagca aatacggcgc
    13861 ataagatgca ttgctttcgg cgacgttgaa caatgtcgca ggctgcacat gccccagtag
    13921 gctaccgaag gtgaggaaga aggtaatgac tttcgccccc agtacaatcg ccgtcatgcg
    13981 gctgacggcc ttagtactca accacaccac aaacgctacc agcagtgcaa aaccaaaacc
    14041 cgccgcccgt gccgggacgt ttagcgacat ctctgcgaag gtgtgatgca gaattgaacc
    14101 actggcagaa atataggcat aggtcaggat atagagcaca aaggcaatgg aaataccgtt
    14161 gaccacgttc cagcctttgc ccagcaaatc tttggtgatg gtgtcaaaac tcgaaccgat
    14221 tcggtaattc aggttagctt ccagaatcat caagccggaa tgcagcatac agaaccaggt
    14281 aaagatcagc gccgccattg accagaaaaa ccacgccccg gacatgacca ctggcagaga
    14341 aaacatccct gcgccaataa tggtgccgcc gataatcacc acgccgccaa gcagcgacgg
    14401 tgacgtttgg gtggtggtta gtgttgccat gagagcttct ctccagtgaa aaatagtgcg
    14461 actgcgttgt tatgcattgc actgtaccag tacacgagta caaaagacag aaaaaaagcc
    14521 ccgatggtaa aaatcggggc tgtatatatt attttacaga ttgtgttcgc tgttcagcga
    14581 tgattacgca tcaccaccga aacgacgacg accggtagag tcatcacgac gcggagcgcg
    14641 gccttcacga cgttcgccgc taaaacgacg accatcacca cggccacctt cacggcgttc
    14701 accgctgaag ttacgaccgc cttcacgacg ttcgccaccg aaaccacgac caccgccacg
    14761 acgctcaccg ccagtatgcg gctgtgcatc gcccagtaac tgcatgttca tcggcttgtt
    14821 gagaatgcga gtgcgcgtaa agtgttgcag cacttcaccc ggcatacctt tcggcagttc
    14881 gatggtggag tgagaagcaa acagcttgat gttaccaatg taacggctac tgatgtcgcc
    14941 ttcgttagca atcgcaccaa cgatatgacg aacttcaaca ccatcatcgc ggcccacttc
    15001 aatgcggtac agctgcatat cgccaacatc acgacgttca cgacgcggac gatcttcacg
    15061 gtcaccacgc gggccacggt cgttacgatc gcgcggacca cggtcatcac ggtcacggaa
    15121 ttcacgtttc ggacgcatcg gcgcatctgg cggtacgatc agagtacgtt caccctgtgc
    15181 cattttcagc agtgccgcag ccagagtttc gagatccagc tcttcacctt ctgcagtcgg
    15241 ctgaattttg ctcagcagtg cgcggtattg atccagatcg ctgctttcca gctgctgctg
    15301 tactttagcg gcgaattttt ccagacggcg tttgcccagc agttctgcgt tcggcagttc
    15361 tacttccgga atagtcagct tcatagtacg ttcaatgttg cgcagcagac gacgctcgcg
    15421 gttctcaacg aacagcagcg cgcggccagc acgacccgca cgaccggtac gaccgatacg
    15481 gtgaacgtaa gactcagaat ccatcgggat atcgtagtta actaccaggc tgatacgctc
    15541 aacgtccagg ccacgggctg caacgtcggt cgcaatcagg atgtccagac gaccatcttt
    15601 caggcgttcc agtgtctgtt cacgcagcgc ctggttcatg tcaccgttca gcgcggcgct
    15661 gttgtagccg ttacgctcaa gagcttcagc cacttccaga gtcgcgtttt tggtacgaac
    15721 gaagataatc gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg cttcgttttt
    15781 gcgcataccc cagacagtcc agtagctctg gctgatgtca ggacgggtag tcacgctgga
    15841 ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg gtaatgcgac gaatcgcttc
    15901 cggcatggtt gcagagaaca gagcggtctg atgaccttcc gggatctgcg ccataatggt
    15961 ttcaacgtct tcgatgaagc ccatgcgcag catttcgtca gcttcatcca gaaccagacc
    16021 gctcagttta gagaggtcca gagtgccacg tttcaggtgg tccagcagac ggcccggagt
    16081 accgacaacg atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat aacgctggcc
    16141 gccgtacaga gcaaccacat ttacgccgcg catgtgttta gagaaatccg tcattgcttc
    16201 agcaacctgt accgccagtt cgcgggtcgg tgccagcacc agaatctgtg gtgctttcag
    16261 ctcaggatca agattctgca acagaggtaa agagaatgct gcagtttttc cgctccccgt
    16321 ctgggccata cccagaacgt cgcggccatt cagcagatgt ggaatacact ctgcctgaat
    16381 tggagatggt ttttcgtaac ccagatcgtt aagggcttca aggataggag ccttcaggcc
    16441 cagatctgca aaagtggttt cgaattcagc catgtagtac gtgtgcctca aaattaatgg
    16501 cggccagtct acataactca tcatgaaatt gatcagcaat tttcattgaa aagtgtgaac
    16561 cggctcaaag taggtgtatt aacgaacaac aacgccctca cccgttaagg tgatggcaat
    16621 caaaaaagat tacgggctga tgtgtacgtc agctattgct ggtccgattc tgccaggtca
    16681 tcttggtcct ggcccaggag cgataattcc aacaatgcgt atcggtgctc aacaaagtta
    16741 tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa atcccccaga
    16801 cttaggtagt acttacctaa atagaagttg gtttcactga gatgctcagc gagcgaggtg
    16861 ttatccgttg cgtccgcctt gagcctttcc attaacgttt gttcgctaat gttgcccaag
    16921 tagaactcga caatgttcca tccccactgt tccttatccg atttttcgaa gtgctgtttc
    16981 aacacttctt tagcctgctt ctcatcgagc ttctgctcgg cgagataaag ccacagacta
    17041 cggaaaggat cattgggatc gtcttgataa aacgccagca gatcatcttg cgctaacttg
    17101 tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt gtaagttgga
    17161 tcaagctcaa gtacagaatc aaacgcttca taggcagcat caaaattgcc tgcctgcgtt
    17221 aaatatatgc ctaagtaatt gaatacttca ggcatatccg gtcggattgc cagcgcttgc
    17281 gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat catacaacac tccgcgctca
    17341 tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat ttgttccata
    17401 cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag gacttcactt
    17461 ttacgccagg aagtattact gcatcctgca agcgtaagtg ctgtcgcaac gaaacaccag
    17521 cgcaaaaaag gcttcatttc ccactcccga agaccacggt tgaatgaacg tcctgttccc
    17581 ggttgctaac aaggcgtcct gcccggttaa aagccccccg ccgcagcgga gggcaaatgg
    17641 caaccttact cgccctgttc agcagccgga gcttccggtg ctgcagcagg ttgagactgc
    17701 tcagtcgctt ctttaatgct cagacggata cggccctggc gatcaacttc tagaactttc
    17761 accggtactt cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg tttgtcagcg
    17821 atttgagaga tgtggaccag accttcttta ccgccgccga tggcaacaaa tgcgccaaag
    17881 tcaacgatac gggtcacttt accagtgtag acgcggccca cttcgatttc tgcagtgatc
    17941 tcttcgatac gacgaatagc atgtttcgct ttctcgccgt cggtcgctgc gatcttcaca
    18001 gtaccgtcat cttcgatttc gatggtagtg ccagtttctt cggtcagggc acggataaca
    18061 gagccgcctt taccgataac gtctttgatc ttatccgggt tgatcttgat ggtatggata
    18121 cgcggtgcga actcagagat atcgccacgc ggcgcgttga tcgcctgttc cattacgccc
    18181 aggatatgca gacgcgcacc tttagcctgg ttcagcgcaa cctgcatgat ctctttggtg
    18241 ataccttcaa ttttgatatc catctgcagt gcagagatac cgtcgcggga acctgcaact
    18301 ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag tacaacgtag
    18361 ttgtcgcctt ctttcaccag acccattgcg atacccgcaa cggcagcttt gattggcaca
    18421 cctgcgtcca tcagcgccag agacgcgccg cacacggaag ccatagaaga agaaccgttg
    18481 gattcggtga tttcagacac tacacgtacg gtgtacggga atttgtccat atccggcatg
    18541 actgccagca cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg cttcggagaa
    18601 ccgaccatgc cggtttcgcc tacggagtac ggagggaagt tgtagtggaa caggaaggta
    18661 tcggtacgtt cgcccatcag ttcatcaaga acctgcgcgt cacgagcggt acccagcgtt
    18721 gcagtaacca gtgcctgcgt ttcaccgcgg gtgaacagcg cagaaccgtg agtacgcggc
    18781 agcacgccag tacgcacatc cagaccacgg atcatatctt tttcacgacc gtcgatacgc
    18841 ggttcgcctg ccagtacgcg gctacgaaca acgtttttct cgatagcgtg cagaatttca
    18901 cccagttcgt tttcgtccag ggtttcgtct tcagcaagca gcgtcgcgat ggtttcagat
    18961 ttgatgacat caacctgcgc ataacgctct tgtttgtcgg tgatgcggta agcatcactc
    19021 agacgagctt cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac cggctccggc
    19081 tgccagtccc aacgcggttt accggcttct ttcactagtt cattgatgtt ctgaataaca
    19141 acctgctgtt gttcatgacc gaacactact gcgcccagca tctgatcttc gctcagcagt
    19201 tcagcttcag attcaaccat cagtacagcg gcttcagtac cggcaacaac cagatccagt
    19261 ttgctctctt tcagctcgtc ctgagtcggg ttcagtacgt actggtcatt gatgtaacct
    19321 acgcgggcag caccaatcgg gccattgaac ggaataccag acagagacag tgctgcggaa
    19381 gcaccaatca tcgcgacgat atccgggtta acctgcgggt taacagaaac cacggtggcg
    19441 ataacctgaa cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg gcggtcaatc
    19501 agacgcgcga tcagggtttc gccttcgctt ggacgacctt cacgacggaa gaagctaccc
    19561 gggatacgac cagcagcgta ggtacgctcc tgatagttaa cggtcagtgg gaagaagtcc
    19621 tgacctggtt tggctttttt ctgtccaaca acggtaacga ataccgcggt gtcatccatg
    19681 ctaaccataa cagcggcagt agcctgacga gccatcatgc cggtttccag agtcacggtg
    19741 tgttggccgt actggaattt acgaacgatc ggattaagca atgtaatatc ctttctcttt
    19801 cttagacagt accttacggc actggtgtta atacccgatc ttctgcgcat cctcgcgact
    19861 aatgacaacc ctaacccagc tctatgtggg taaagcctct cattagccgc gcgaacctct
    19921 gcaacggaag atcattcata gcaacaatac attagtttcc agtgaattgc tgccgtcagc
    19981 ttgaaaaaag gggccactca ggcccccttt tctgaaactc gcaagaatta gcgacgcaga
    20041 cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc
    20101 agtttacgac gctgagaaac catgcgcagc agaccacgac ggctgtggtg atcttttttg
    20161 tgctctgcaa agtggccctg caggtggttg atctgtgcag tcagcagtgc tacctgaact
    20221 tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt agctgttgct
    20281 tcagtactta gagacatttt aaaactccaa agtatataga atgaaaggac gccgatctct
    20341 aattcagcga tcccagtgta cgttacgcaa agtgttaaac aatttacgcg acgttaagcg
    20401 gcagtattct actcgtagcg acctgttatc gcaagacggt taacattacg ccgggtattc
    20461 aaccaccagg cgacgaggcg cgacgcggcc ttcatcgtca atttcgccca taccgataaa
    20521 cttgccgttc tcaccttctg taacgcgaac cagtccttcc agcggcgcac cggatgtacg
    20581 aactgggtta ccatttttga agtaaacaga agacgttaac ggaagattca ccaccgggta
    20641 gtccgaagct ggactgtcca ttggcatcag taatggatca agtaactccg cagccggaat
    20701 atcctgctgt tcggcttgtt cgaccagttc acgcagatgt tccagggtca ccatccgttc
    20761 aaccggatat ttacttaccg ccagacgacg caggtaaata acatgcgcgc cacaaccgag
    20821 tttttcaccc aggtcatcaa tgatggtgcg gatataagtg ccttttgagc agtgaatttc
    20881 cagctccagc tcattgcctt cgtggcgaat aaacagcaat tcataaacgg taatcgggcg
    20941 cgcttcacgc ggtacttcaa tgccctgacg cgcatattcg tacagttttt taccctgata
    21001 tttcagtgct gaatacatcg aagggatctg ttcgatatcg ccacggaaag tatccagcgc
    21061 cgccgccagt tgctctgcgc taaaggttac cggacgttct tcaacgattt gtccgtcggc
    21121 atcagaagta tcggtacgct gtccaagacg cgcaatgacc cgatagcgtt tgtcggagtc
    21181 cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa attggcaaca tgcctgttgc
    21241 cagcgggtcc agcgcaccgg tatgcccggc acggttggcg ttataaatcc gtttcacttt
    21301 ttgcagcgca tcattgctgg acataccctg cggtttatcc agcaacaaaa cgccgttaat
    21361 gtcgcgaccg cgacgacgag gacgactcat tagtcctcct tgctgtcgtc cgggttaaca
    21421 cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg catcccttca
    21481 accagagagt tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg catcgctttc
    21541 cccagcaggc tgcggatgaa accagaagct tcttgcaacg ctttgatgcc cgctttaacc
    21601 gcgtcttcat ctttgtcgtt gaggaacgtc acatatactt tggcatacgc caggtcgcga
    21661 gacatttcga caccggaaac ggtggtcatc atgcccaggc gaggatcttt aatttcacgc
    21721 tgcaggatga gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat
    21781 tctttcgcca taataaattc tcctgacaaa aaaggggctg ttagcccctt tttaaaatta
    21841 atttcaggtg gaagggctgt tcacgttggc ctgataagac gcgccagcgt cacatcaggc
    21901 aatccatgcc ggatgcggcg taaacgcctt atcccgcatg gaaccctaaa aaccttaagc
    21961 gatggtacgt tggatctcga tgatttcgaa tacttcgatc acatcgccag tgcggacgtc
    22021 gttgtagttc ttaacgccga taccacattc cataccgtta cggacttcgt taacgtcatc
    22081 tttgaagcgg cgcagggact ccagctcgcc ttcgtagata accacgttgt cacgcagaac
    22141 gcggatcggg ttgtgacgtt taaccacacc ttcggtaacc atacagcctg cgatagcacc
    22201 aaatttcggc gatttgaaca cgtcacgaac ttccgccaga ccgataatct gctgtttcag
    22261 ttccggagac agcataccgc tcatcgccgc tttcacttcg tcaatcaggt tatagatgac
    22321 ggagtagtaa cgcagatcca ggctttccgc ttcaatcact ttacgtgcag aggcatcagc
    22381 acgtacgttg aagccaacca ggatggcgtt ggacgccgca gccagggtgg cgtcggtttc
    22441 ggtgatacca cctacgccag aaccgatgat cttcacttta acttcgtcag tagacagttt
    22501 cagcaaggag tcggagatcg cttccacaga accctgtacg tctgccttca ggacgatgtt
    22561 cacttcgtga acttcgcctt cggtcatgtt ggcgaacatg ttctcgagtt tagatttctg
    22621 ctgacgcgcc agtttaactt cgcggaattt accctgacga tacagtgcaa cttcacgcgc
    22681 tttcttctcg tcacgtacaa cggtaacttc atcacccgca gccggtacgc cggacaggcc
    22741 gaggatttcc accggaatgg acggacccgc ttccagcact tcctgaccca gttcgttacg
    22801 catcgcacga acacgaccgt attcgaagcc acacagaacg atatcgccct tgtgcagagt
    22861 accttcacgt accagaacgg tagcaaccgg accacgacct ttatcgagga aggattcgat
    22921 aaccgcaccg ctcgccatac ctttacgtac cgctttcagc tccagaactt ccgcctgcag
    22981 caggatagcg tccagcagtt catcgatgcc ggtacccgct ttcgcagata cgtgtacgaa
    23041 ctggctttca ccgccccact cttccggcag gatgccatac tgggagagtt cgttcttaac
    23101 acgatccgga tcggcttctg gtttatcaat cttgttcact gcaaccacca ccggtacctg
    23161 cgccgctttc gcgtgctgga ttgcttcgat ggtctgcggc atcacgccgt cgtcggcagc
    23221 aacaaccagg actacgatgt ccgttgcctg cgcaccacgt gcacgcattg aagtaaacgc
    23281 ggcgtgcccc ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc
    23341 accaatgtgc tgggtaatgc cgcccgcttc gccagaggca actttcgttg aacgaatgta
    23401 gtccagcaga gaggttttac cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg
    23461 cggttcagcc gcagcacccg tgtcacggtc gctcattacc gcctcttcca gctcgttttc
    23521 acgacgcagg ataactttgt ggcccatctc ttcagcaacc agctgtgcgg tttcctgatc
    23581 gataacctgg ttgatggttg ccattgcgcc cagtttcatc atcgctttga tgacctgaga
    23641 gcctttaacc gccatcttgt tcgccagttc gccaacggtg atggtttcgc cgatcacaac
    23701 gtcacggtta acggcctgag ctggcttctg gaagccttgc tgcagggaag aacctttacg
    23761 ttttccgcct ttaccgccac gtactgctgc gcgtgcttct tcacgatcag cttttgattc
    23821 agcgtgtttg ttgcctttct tcggacgcgc tgctttcgcg ttacgaccac ggccacgacc
    23881 gccttcgact tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag aagtagtgac
    23941 gtgataatcg ctggaatctt cagtcggttc cgcgttatca gtccatttgt tttcttccgc
    24001 catacgacgt gcttcttcag caacgcgacg tgcttcttct tcgagtttac gatgcgcttc
    24061 ttcttcggct ttacgcttga gctctgcagc ttcctgctca cggcgggctt tttcagcctg
    24121 ggcgttttta gtcatatcgt cttgttgatt gctcactttg tctttttccg cagcttcacg
    24181 tttcgcttgt tcagcagctt cacgcttagc ttgttctgca gcctcacgtt cagctttttg
    24241 ttgcgcctcg cgtttagccg attcttctgc ctcacgacgg gcttgctctt ccgcttcacg
    24301 ctgcgcttgc tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt
    24361 gcgtttcttg cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt
    24421 aagggtgctg cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt
    24481 caggtggtca atcaaagtct gtttctcttg tgcagacaca gagtcgtcag cagacttccg
    24541 gatacctgca tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc
    24601 cagcgtttta atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc
    24661 gtcaccgaac cagcaaatat tacgggcagc cataatcagt gctccggcct tttcgtcggt
    24721 caacccttcg atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca
    24781 aacgccacgg gcggccagtt tgaatgccaa atcacgatct accccttcaa ggttcagcag
    24841 atcgtcagcc ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa tggtggccag
    24901 tgcattttta gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc
    24961 caacagctct ttcatcggca cataggccag ttcttccagc gtcgagaagc cttcttctac
    25021 cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc
    25081 gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg gtcatcacgt tgagttccca
    25141 accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag
    25201 gttaccggct tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc
    25261 aacgtctgcc ggtgccattg cgttaatcac gaactgcgcc gggttatcat cccacaggac
    25321 gatatcgata cgctcgccac ccagttcagt agacaccgcc tgaacacgcg cgccacgcat
    25381 acctacgcaa gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc
    25441 acgagaaccc ggatcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttctgg
    25501 cacttcaata cggaacagtt cgatcagcat ttccggcttg gaacgagtga cgaacagttg
    25561 cgcgccacgc gcttccgggc gaacggaata aagcacgcca cgaacgcggt cgccagggcg
    25621 gaagttttca cgcggcagca tatcttcacg caggatcacg gcttcagcgt tgttgcccag
    25681 atccagagag atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc
    25741 gtgttcacgg aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat
    25801 aacctgtttt gccgtctggg tagtgatacg gtcaaaggta acagactcaa tctgatcttc
    25861 aacgtaatcg cccaggttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc
    25921 cttggtcggc tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc
    25981 gcttttgcga tcgatctgta cgcggacgtc gatctcttgt tcatattttt tctttgttgc
    26041 tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt
    26101 ggatacggct tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc
    26161 agactattaa aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc
    26221 atctttacct tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg
    26281 ccatttacga cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa
    26341 acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag
    26401 gttataagca acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac
    26461 atcagcacaa tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga
    26521 tgtgcgaccg cgaataaatt cgatgccaac cagttcaaaa cccagggcct caactggcgc
    26581 agtaatcatc tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa
    26641 aaagggccta aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg
    26701 ctttatataa ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt
    26761 tttcaaatcc cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg
    26821 gaaagtggtt gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc
    26881 taccaggctg ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa
    26941 atgcaaatac tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata
    27001 gtgatccacg ccacattttg tcaacgttta ttgctaatca tgtgaatgaa tatccagttc
    27061 actttcattt gttgaatact tttgccttct cctgctcccc ct
//
LOCUS       DAETQN010000050        26843 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.050, whole genome shotgun sequence.
ACCESSION   DAETQN010000050 DAETQN010000000
VERSION     DAETQN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26843
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.050"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            202..474
                     /locus_tag="K0W68_002109"
     CDS             202..474
                     /locus_tag="K0W68_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBI3073662.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="K0W68_002110"
     CDS             567..650
                     /locus_tag="K0W68_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073663.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="K0W68_002111"
     CDS             750..1571
                     /locus_tag="K0W68_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBI3073664.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="K0W68_002112"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="K0W68_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBI3073665.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="K0W68_002113"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="K0W68_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBI3073666.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="K0W68_002114"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="K0W68_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBI3073667.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="K0W68_002115"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="K0W68_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBI3073668.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="K0W68_002116"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="K0W68_002116"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBI3073669.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /locus_tag="K0W68_002117"
     CDS             7217..8161
                     /locus_tag="K0W68_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI3073670.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="K0W68_002118"
     CDS             8347..9729
                     /locus_tag="K0W68_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI3073671.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="K0W68_002119"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="K0W68_002119"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBI3073672.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="K0W68_002120"
     CDS             complement(10485..10820)
                     /locus_tag="K0W68_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBI3073673.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="K0W68_002121"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="K0W68_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBI3073674.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="K0W68_002122"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="K0W68_002122"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBI3073675.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="K0W68_002123"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="K0W68_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBI3073676.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="K0W68_002124"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="K0W68_002124"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBI3073677.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15521..17167)
                     /gene="fumB"
                     /locus_tag="K0W68_002125"
     CDS             complement(15521..17167)
                     /gene="fumB"
                     /locus_tag="K0W68_002125"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBI3073678.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17366..18541
                     /gene="manA"
                     /locus_tag="K0W68_002126"
     CDS             17366..18541
                     /gene="manA"
                     /locus_tag="K0W68_002126"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBI3073679.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18642..20150
                     /locus_tag="K0W68_002127"
     CDS             18642..20150
                     /locus_tag="K0W68_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBI3073680.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20331..21641)
                     /gene="uidC"
                     /locus_tag="K0W68_002128"
     CDS             complement(20331..21641)
                     /gene="uidC"
                     /locus_tag="K0W68_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBI3073681.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21680..23053)
                     /gene="uidB"
                     /locus_tag="K0W68_002129"
     CDS             complement(21680..23053)
                     /gene="uidB"
                     /locus_tag="K0W68_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HBI3073682.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGFCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(23050..24861)
                     /gene="uidA"
                     /locus_tag="K0W68_002130"
     CDS             complement(23050..24861)
                     /gene="uidA"
                     /locus_tag="K0W68_002130"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBI3073683.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25250..25840)
                     /gene="uidR"
                     /locus_tag="K0W68_002131"
     CDS             complement(25250..25840)
                     /gene="uidR"
                     /locus_tag="K0W68_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBI3073684.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(26061..26828)
                     /gene="hdhA"
                     /locus_tag="K0W68_002132"
     CDS             complement(26061..26828)
                     /gene="hdhA"
                     /locus_tag="K0W68_002132"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HBI3073685.1"
                     /translation="MRHAPCAMRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6737 a         6950 c         6455 g         6701 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgat ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tagctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggccgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatga aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc ggctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg gcggctctgt ttatttcaca cagcgagtgc
    15541 actgtgtgag ttgtatctgc tggaagaagt catttccttt atcatccaca aggataaacg
    15601 ccgggaaatc ttccacttca attttccaga tggcttccat tcccagttcc ggatattcaa
    15661 cacattccag gctcttaata cttccctgcg ccaatacagc ggccggacca ccgatactgc
    15721 caaggtagaa gccgccgtgt tttttacagg catccgtcac ctgctggctg cggttgcctt
    15781 tcgccagcat gatcatactt ccgccctgcg cttgcagttg atcgacataa gaatccatcc
    15841 gtccggcggt cgttgggcca agagaaccgg aggcataacc ttccggcgtt ttggccggac
    15901 ccgcgtagta aatcggatga tctttaatgt actgcggcag cccttcaccg ttatccatcc
    15961 gctctttcag tttggcgtga gcaatatcac gaccgacgat aatcgtgccg ttaagcgata
    16021 agcgtgtaga aacgggatac tgcgacaact gtgcgaggat ctctttcatc ggacggttaa
    16081 ggtcaacgcg caccgcttcg ccttctcccg ctttacgcag ctcttcaggg atatatttac
    16141 ctggattatg ttccagtttt tcgatccaga tcccctgacg gttgatcttc gctttgatat
    16201 tacggtcagc agagcaggaa acacccatac caaccggaca ggatgcgccg tgacgtggca
    16261 ggcgaatcac gcgtatgtcg tgagcgaagt atttaccacc aaactgcgca cccagaccga
    16321 gattttgcgc ttcgatcagc aattcttttt ccagttccac atcgcggaat gcctgaccgt
    16381 gctcattccc ttccgttggc agttcgtcat agtatttagc ggaagccagt ttcactgttt
    16441 taaggttagt ttctgcagaa gttccaccaa ttacgaacgc aatatgatac ggaggacagg
    16501 ccgccgtacc cagcgtgcgc atcttctcaa ccaggtaatt tttcagtttc cccggcgtta
    16561 gtaacgcttt ggtttcctga tagagatacg tcttgtttgc cgaaccacca cctttggcga
    16621 tacagaggaa tttgtactcg tcgccatcaa cggcataaag atcgatctgc gctggcagat
    16681 tggtgccggt attcacctct ttatacatat ccagcggcgc gttttgcgag tagcgcaaat
    16741 tatcttcgat ataagtgtta tagacaccgc gcgccagcgc cgcttcatca cagccaccgg
    16801 tccatacacg ctgccctttt ttaccaacaa taatcgcagt gccggtatcc tgacaggttg
    16861 gcagaacacc tttcgccgcg atgtcggagt tgcgcaggaa ttgcagcgcc acatatttat
    16921 cattttcgct ggcttccggg tcacgcagaa tgtcggccac ctgttgttgg tgagccggac
    16981 gcagcataaa tgacgcatca tgaaacgcct gacgcgccaa cagagttaac gcttcgggtg
    17041 cgactttcaa aatctcctgc ccttcaaatt cagatacgct aacgtgttcg ctggttagca
    17101 ggtaatactc agtatcatct tttttgagtg gaaaaggagc ctgataatga aagggtttgt
    17161 ttgacattgt tctctcactt actgcctggt ttggttatgc tctgggcggg tgttccgttg
    17221 ccctgttaaa agcgagtaac aatatcctac acactttttt aacaaaaact gagactagta
    17281 cgactttttg cggctccagg ttacttcccg taggattctt gctttaatag tgggattaat
    17341 ttccacatta aaacagggat tgatcatgca aaaactcatt aactcagtgc aaaactatgc
    17401 ctggggcagc aaaacggcgt tgactgaact ttatggtatg gaaaatccgt ccagccagcc
    17461 gatggccgag ctgtggatgg gcgcacatcc gaaaagcagt tcacgagtgc agaatgccgc
    17521 cggagatatc gtttcactgc gtgatgtgat tgagagtgat aaatcgactc tgctcggaga
    17581 ggccgttgcc aaacgctttg gcgaactgcc tttcctgttc aaagtattat gcgcagcaca
    17641 gccgctctcc attcaggttc atccaaacaa acgcaattct gaaatcggtt ttgccaaaga
    17701 aaatgccgca ggtatcccga tggatgccgc cgagcgtaac tataaagatc ctaaccacaa
    17761 gccggagctg gtttttgcgc tgacgccttt ccttgcgatg aacgcgtttc gtgagttttc
    17821 cgagattgtc tccctactcc agccagtcgc aggtgcacat ccggcaattg ctcacttttt
    17881 acaacagcct aatgccgaac gtttaagcga actgttcgcc agcctgttga atatgcaggg
    17941 tgaagaaaaa tcccgcgcgc tggcgatttt aaaatcggcg ctcgacagtc agcagggtga
    18001 accgtggcaa acgattcgtt taatttctga attttacccg gaagacagtg gcctgttctc
    18061 cccgctgtta ctgaatgtgg tgaaattgaa tcctggcgaa gcgatgttcc tgttcgctga
    18121 aacaccgcac gcttacctgc aaggcgtggc gctggaagtg atggcgaact ccgataacgt
    18181 gctgcgtgcg ggtctgacgc ctaagtacat tgatattccg gaactggttg ccaatgtgaa
    18241 attcgaagcc aaaccggcta accagttgtt gacccagccg gtgaaacaag gtgcagaact
    18301 ggacttcccg attccagtgg atgattttgc cttctcgctg catgacctta gtgataaaga
    18361 aaccaccatt agccagcaga gtgccgccat tttgttctgc gtcgaaggcg atgcaacgtt
    18421 gtggaaaggt tctcagcagt tacagcttaa accgggtgaa tcagcgttta ttgccgccaa
    18481 cgaatcaccg gtgactgtca aaggccacgg ccgtttagcg cgtgtttaca acaagctgta
    18541 agagcttact gaaaaaatta acatctcttg ctaagcttat taaaggctta taacaccttc
    18601 aggcggccag tccgcctgat ttcattttat ggataatcat tatgaataaa tcgctggtag
    18661 cggtaggcgt cattgttgcg ctaggcgtag tctggacagg cggcgcatgg tatacaggca
    18721 agaagattga aacccatctc gaagacatgg tcgcgcaggc gaacgcgcaa ctcaaactga
    18781 cagctcctga atccaacctg gaagtgagtt atcaaaacta tcatcgcggc gtattcagca
    18841 gccagttgca actgttggtg aaacccattg ccgggaaaga aaatccgtgg attaaaagcg
    18901 gtcagagcgt catcttcaac gaatcggttg atcatggtcc cttcccgctt gcccagctta
    18961 aaaaactgaa cctgatcccg tcgatggcat caattcaaac cacgctggtt aataacgaag
    19021 taagcaaacc actgtttgat atggcaaaag gtgaaacgcc ttttgagatt aactcgcgca
    19081 ttggttacag cggtgattcc agttccgata tttcgctcaa gccactgaat tacgagcaaa
    19141 aggatgaaaa agtcgccttt agcggcggcg agttccagtt aaatgctgac agagacggca
    19201 aagccatctc cctttccggg gaggcgcaaa gtggtcggat agacgcagtt aacgaataca
    19261 accagaaagt gcagttgacc tttaataatc tgaaaaccga cggttccagc acgctggcaa
    19321 gttttggtga gcgcgtagga aaccaaaaac tgtcactgga aaaaatgacc atttcagtgg
    19381 aaggcaaaga actggcactg ctggaaggca tggagatcag cggtaaatcg gatctggtca
    19441 atgacggtaa aacgatcaat agccaactgg attactcgct aaacagcctg aaggtacaga
    19501 atcaggatct gggcagcggc aagctgactt taaaagtcgg ccagattgat ggtgaagcct
    19561 ggcatcagtt tagccagcaa tataacgcgc aaactcaggc gctgctggca cagccagaaa
    19621 ttgccaacaa tcccgaactt tatcaggaga aagtgacgga agccttcttt agcgccctgc
    19681 cgctgatgtt gaaaggcgat ccggtgatta ctatcgcgcc gctaagctgg aaaaacagtc
    19741 agggtgaaag tgcgctgaat ctgtcgctgt tcctgaaaga tccggcaacg actaaagaag
    19801 cgccgcaaac gctggcgcag gaagtagatc gttcggttaa atctctggat gcgaaactga
    19861 ccattccggt ggatatggca actgagttta tgactcaggt agcgaagctg gaaggttatc
    19921 aggaagatca agcgaaaaaa ctggcgaaac agcaagttga aggtgcatca gcaatggggc
    19981 agatgttccg tctgaccacc ttgcaggaca ataccatcac caccagcctg caatatacta
    20041 acggtcagat aacgttaaac gggcagaaaa tgccactgga agatttcgtt ggtatgtttg
    20101 cgatgccagc tcttaacgtt ccggctgtac cggcaattcc gcagcagtaa ttcacggcca
    20161 aaacgccgga gaattttcac ttcggcgttt tcatctgaaa acaaaccaga gattgcctga
    20221 tgcgctacgc ttatcaggcc taccttttcc tgcaacactt tgaatttatg agtttttgta
    20281 ggctggataa ggcgtttaca ccgcatccgg cataaaaaac gcgcactttg tcaacaatct
    20341 gaaacgccgg aggtttttct ctccggcgtt atttctgact tcagcataaa gtcatactga
    20401 atactcattt tcacatccca gtaattcagg cgatcgccca gcgtactgcc atcgcgcttt
    20461 tcccgccctt tatcggcagc aagaaagaaa ttcatccctt gtaacggccc ttcgctgacc
    20521 cgatagttca taccacctaa gatacccgcg ccttcaatcc gcacatctcg tttactggat
    20581 tcatagccgc gagtcacaat ctgtgcaaat gtcagtgtaa attgcggcgt taaacgatag
    20641 ttgacgccca attgccagga ctgcattcct tcacggttac gatcaagact taatgaaaag
    20701 ggatagccaa tgtcggtatc caccacggca ccattcattg catggcgcaa ataaccacca
    20761 ctgccaaata cgcttcccca cggcgcattc caggtcaatt gtccactaac caacgccgtt
    20821 tcattgggct ggctggtatt acgacttaaa ccttcgagct gcgcgtaaaa gccgagcaac
    20881 tcccctttca acgtataatt cgggtcgagc ggtatgcttt tctcgagaat actttgtatc
    20941 tgattggaaa catctttctg taccgatgcc cccagagaat agcgactggt atcatcatgc
    21001 caactggcgg ctaaaaaaga acgtgcgcga tgtttatcgt attttcccgt ctggctgtta
    21061 tacacccctt catcacgaat accggttgtt gaatcagtcc ataaaaatac tcgtgaacgc
    21121 cacattcccc acagatcaat atttggtgtt aaagcccaat aacctaatgc tccttcagaa
    21181 actgtcggta atgtacgttg cgaactgcca gccattaacg gaatacgata aggtaagcta
    21241 ccataactat aatccgtccc gaagcgacct gatttaagca ggctattttc actgggtttt
    21301 atttttactg cccccagggc aaagccaaaa cttttatcac catcaagata ccaccgggta
    21361 ctcatatcag cacgagcacc taatttataa acataatatg ccccgccttc aacgccaata
    21421 atattggaat aataaccact attgaaatcg agcaatcctc cctgtaccca tgcgtaaata
    21481 tctcttcccg cgccagcact gggcttatcc gccctgcgca attcgttttt cagtctgatg
    21541 cgtagtcccg cctcatcgtc agccagttgc gccgcataag cagagataag gccagaggca
    21601 gccgtcaggc aaataacggc catggccact atttttctca tagttgcacc tttgatgttc
    21661 tgattatttt tattgattat taattagtga tatcgctgat taattgctgc tgcacttttt
    21721 tacgattatc aatttcaacc acgatttctt tgaatttttt atccgtaagc ggataaaacc
    21781 agataataac gaatgccagt agcataaatc cgcaaggtac taaggcaatt gatgtgcgga
    21841 tgcccataat aacttccggc gtttgcacct gattggcaat atatccgctt aaccccaaaa
    21901 taaaggcagg aattgaacct ccgattgcct gaccgcattt acgggtaaat gagaatagtg
    21961 aataggtgag cccttcaatt cgcacgccgg tcaggtattc accgtattct acggtatcag
    22021 cttccagtgc ccacatcact gtcatagtaa cgccctgacc aattgaagcg atggccaacg
    22081 caaccaacgc caccggcagt gaccagacgg aaacccagaa gaatagcaga taaccgcagg
    22141 ttcccagcaa agcgccaatc aggaaggtat tctttttacc gatcctcgcg accatccccg
    22201 gcacaagcgg tgccgatgcc acagtaccaa ccaggttttg caccagtacc agcacagtga
    22261 acagcccggt atcatttaac acatagcgca cgtagaacaa cgacgaggcg ctgaccgcaa
    22321 aggtcgaaat cagcacacac agcgcaccga tgcacaacat aaacagcggg cgattccgtt
    22381 tcagggtttg caaactgatc ttcaatgacg gctgcgcaac gatacgtacc acattctcac
    22441 gcgtcgattt gaagcagatg aagtaaagca ccattccggc aatcgccagc acaatcgtcc
    22501 agaaatggta taccgacacc atctcttccg ggctggagtt cttaatgctc ggccctatca
    22561 gaaatgccag gcagacaaag gtcaatgaag cggcaatccc acgagccgcg cccagacggg
    22621 cgcgggattg tggttgttgg gtcatcgcgg tagcaagtga accataagga atattcacca
    22681 ggctgtagca aaacccgagg cccatgtagg tcaaatatgc atacactact ttgcggctat
    22741 ggctccagtc ggtcggtacc cagaatacca gcacgctgaa gatcattaac ggcgcagtac
    22801 cgaagagtaa aaacgggcgg aattttcccc agcgggtatt cacactgtcc accactcgtc
    22861 cggcaaagac gtcggcgaag gcatcgaata cccgcaccag taacagcatg gtgcccgccg
    22921 cagcggcacc gacgccagcg acgtcggtgt agtaactcaa caggaagagc acccccattg
    22981 cgaaggcgaa gttattggcg acgtcaccga ggctgtagcc gacgatggtg cgccaggaga
    23041 gttgttgatt cattgtttgc ctccctgctg cggtttttca ccgaagttca tgccagtcca
    23101 gcgtttttgc agcagaaaag ccgccgactt cggtttgcgg tcgcgggtga agatcccctt
    23161 cttgttaccg ccaacgcgca atatgccttg cgaggtcgca aaatcggcga aattccatac
    23221 ctgttcaccg acgacggcgc tgacgcgatc aaagacgcgg tgatacatat ccagccatgc
    23281 acactgatac tcttcactcc acatgtcggt gtacattgag tgcagcccgg ctaacgtatc
    23341 cacgccgtat tcggtgatga taatcggctg atgcagtttc tcctgccagg ccagaagttc
    23401 tttttccagt accttctctg ccgtttccaa atcgccgctt tggacatacc atccgtaata
    23461 acggttcagg cacagcacat caaagagatc gctgatggta tcggtgtgag cgtcgcagaa
    23521 cattacattg acgcaggtga tcggacgcgt cgggtcgagt ttacgcgttg cttccgccag
    23581 tggcgcgaaa tattcccgtg caccttgcgg acgggtatcc ggttcgttgg caatactcca
    23641 catcaccacg cttgggtggt ttttgtcacg cgctatcagc tctttaatcg cctgtaagtg
    23701 cgcttgctga gtttccccgt tgactgcctc ttcgctgtac agttctttcg gcttgttgcc
    23761 cgcttcgaaa ccaatgccta aagagaggtt aaagccgaca gcagcagttt catcaatcac
    23821 cacgatgcca tgttcatctg cccagtcgag catctcttca gcgtaagggt aatgcgaggt
    23881 acggtaggag ttggccccaa tccagtccat taatgcgtgg tcgtgcacca tcagcacgtt
    23941 atcgaatcct ttgccacgca agtccgcatc ttcatgacga ccaaagccag taaagtagaa
    24001 cggtttgtgg ttaatcagga actgttcgcc cttcactgcc actgaccgga tgccgacgcg
    24061 aagcgggtag atatcacact ctgtctggct tttggctgtg acgcacagtt catagagata
    24121 accttcaccc ggttgccaga ggtgcggatt caccacttgc aaagtcccgc tagtgccttg
    24181 tccagttgca accacctgtt gatccgcatc acgcagttca acgctgacat caccattggc
    24241 caccacctgc cagtcaacag acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt
    24301 gatatcgtcc acccaggtgt tcggcgtggt gtagagcatt acgctgcgat ggatcccggc
    24361 atagttaaag aaatcatgga agtaagactg ctttttcttg ccgttttcgt cggtaatcac
    24421 cattcccggc gggatagtct gccagttcag ttcgttgttc acacaaacgg tgatacgtac
    24481 acttttcccg gcaataacat acggcgtgac atcggcttca aatggcgtat agccgccctg
    24541 atgctccatc acttcctgat tattgaccca cactttgccg taatgagtga ccgcatcgaa
    24601 acgcagcacg atacgctggc ctgcccaacc tttcggtata aagacttcgc gctgatacca
    24661 gacgttgccc gcataattac gaatatctgc atcggcgaac tgatcgttaa aactgcctgg
    24721 cacagcaatt gcccggcttt cttgtaacgc gctttcccac caacgctgat caattccaca
    24781 gttttcgcga tccagactga atgcccacag gccgtcgagt tttttgattt cacgggttgg
    24841 ggtttctaca ggacgtaaca taagggactc ctcattaaga taaaaatact ggtcaacctt
    24901 taatctgatt agttaaccaa ttctgtgttg cacacagtta tctgtgagaa acccatggaa
    24961 gagaaatgga aataaaaaga actgacaccg cgatcaaaaa accaaaaact gcaataccca
    25021 cagcgtgata ttgatcgcat taatctttaa aacggcgttg acagaagcca taaaataagc
    25081 aaccgaacgt tcgttcgagc atattgggaa cgataaactc tcatgacata tggctacggt
    25141 gaatattttt gggggggaat tctccgatcc cggctagatt gtattttgta cagggaaatt
    25201 aaagtatgaa acgcgggcac agttatcacc agcccgcgaa atgagaggat caggatgcgg
    25261 ttaagatacc gccaatcatc gcgcgtaatc cctgagcaaa cgacgcttca cgcgccagat
    25321 cttccgcttc gatatcggcc agtacaccgt aggtcagatc cagcagtaaa cgtgaagtca
    25381 ttgacgtgtt gatgtctggg cttatctcgc ctttttgctg ggcatcacgc atccgctggg
    25441 caacaaattc cgtgatcgtc atatgcttat ttttaagcat ggcggcgacc tgtgggttac
    25501 gcatcccttc cgccataatt tcgaccacca gcgcccgttg cccaaaggct tcatgggtga
    25561 gagagacaat agactcgacc atatagtcaa caaaatgaat ccctttaatc ggttcccgga
    25621 aacgagccag cgccctctcc tggtcctgta agataatcgc ctgaatcaag gcttctttgg
    25681 agatgaaatg gtgatagagc gtcccgggac taatggcgca agatttacag atggctttca
    25741 tcgaggcact gtgaaatcca ttttctgaaa aaatctctct ggcagcattg aggatccggg
    25801 tccgtgtcgg ttgtgcttca gtctgcatgt tatccatcat taaaccatta tagttagcgt
    25861 gaataactag tttaccgcgt tgtcaggtga gcgcctatca ttgctgttga atagtgcgaa
    25921 ggcacactct attcgtatac ttttcactct gaaattggtt aaccaatagc cgtgatttgg
    25981 ctgccagtta tttagcagag acgttgatga gtttatcccg gtaaacaaca cgcacggttg
    26041 tttaccggtc cgttagtgta ttaattgagc tcctgtaccc caccaccgga gacggtgaga
    26101 atttgtccgc ttacccagct cgcagcaggc gagcaaagga acagcgctgc gttagcaata
    26161 tcttgcggtt ggcccagacg tctgatcggc gtgtgctgta acattttttg ttcaatttct
    26221 ggtgtaataa cggatttcag ggcatcggtt aatattgccc ccggcgcaat gccatttacc
    26281 cgaatatttt tttcacccag gtcaaacgcc atatttctga ccagatgact ggctgcagct
    26341 ttagatgatg cataggaagt catgtttata tttttatttt ctgccgccat agaagtgatg
    26401 gtcagaataa cgccaccgcc atttttttcc atttctggcg caacaagttg tgacagatgg
    26461 aaaaaagaaa acacattcag ttcataagca cggcgaaaat ccgccattgg catatcaaac
    26521 ggtttaggtc caccgccacc ggcgttgtta accagaatat caactttacc cagcttactg
    26581 atagcaaagt ctgccagtgc agagagttcc tgttcggaag taatatcaca acggcaggca
    26641 aatgcctgac cacccagttg ttgaatttcg tctacaacat ggttagctgc gtcggcgtta
    26701 atatcactga ccaccacaga tgcgccagct gtcgcgaatg taatggcgat ttctttacca
    26761 atacctgcac ccgcacctgt gatgatggcg catggcgcat ggcgcatggc gcatggcgca
    26821 tggcgcatgg cgcattttcc gtc
//
LOCUS       DAETQN010000051        26572 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.051, whole genome shotgun sequence.
ACCESSION   DAETQN010000051 DAETQN010000000
VERSION     DAETQN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26572
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.051"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..296
                     /locus_tag="K0W68_002133"
     CDS             <1..296
                     /locus_tag="K0W68_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HBI3073686.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
     gene            791..1690
                     /gene="lysO"
                     /locus_tag="K0W68_002134"
     CDS             791..1690
                     /gene="lysO"
                     /locus_tag="K0W68_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBI3073687.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1834..3486
                     /gene="hcp"
                     /locus_tag="K0W68_002135"
                     /gene_synonym="priS"
     CDS             1834..3486
                     /gene="hcp"
                     /locus_tag="K0W68_002135"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBI3073688.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            3498..4466
                     /gene="hcr"
                     /locus_tag="K0W68_002136"
     CDS             3498..4466
                     /gene="hcr"
                     /locus_tag="K0W68_002136"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBI3073689.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4599..6317
                     /gene="poxB"
                     /locus_tag="K0W68_002137"
     CDS             4599..6317
                     /gene="poxB"
                     /locus_tag="K0W68_002137"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBI3073690.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            6354..7355
                     /gene="ltaE"
                     /locus_tag="K0W68_002138"
     CDS             6354..7355
                     /gene="ltaE"
                     /locus_tag="K0W68_002138"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBI3073691.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            7366..8796
                     /locus_tag="K0W68_002139"
     CDS             7366..8796
                     /locus_tag="K0W68_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBI3073692.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8895..9908
                     /locus_tag="K0W68_002140"
     CDS             8895..9908
                     /locus_tag="K0W68_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBI3073693.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9905..10735)
                     /gene="amiD"
                     /locus_tag="K0W68_002141"
     CDS             complement(9905..10735)
                     /gene="amiD"
                     /locus_tag="K0W68_002141"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBI3073694.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10732..11055)
                     /locus_tag="K0W68_002142"
     CDS             complement(10732..11055)
                     /locus_tag="K0W68_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBI3073695.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(11266..13035)
                     /locus_tag="K0W68_002143"
     CDS             complement(11266..13035)
                     /locus_tag="K0W68_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBI3073696.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNIINNVFKNVSFRSVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFH
                     DCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRY
                     IPQSQDGEDFRSHLQYKLPLSAH"
     gene            13684..14199
                     /locus_tag="K0W68_002144"
     CDS             13684..14199
                     /locus_tag="K0W68_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI3073697.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            14417..15145
                     /gene="artP"
                     /locus_tag="K0W68_002145"
     CDS             14417..15145
                     /gene="artP"
                     /locus_tag="K0W68_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBI3073698.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            15163..15894
                     /gene="artJ"
                     /locus_tag="K0W68_002146"
     CDS             15163..15894
                     /gene="artJ"
                     /locus_tag="K0W68_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3073699.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            15901..16617
                     /gene="artQ"
                     /locus_tag="K0W68_002147"
     CDS             15901..16617
                     /gene="artQ"
                     /locus_tag="K0W68_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBI3073700.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            16617..17285
                     /gene="artM"
                     /locus_tag="K0W68_002148"
     CDS             16617..17285
                     /gene="artM"
                     /locus_tag="K0W68_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBI3073701.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            17577..18308
                     /gene="artJ"
                     /locus_tag="K0W68_002149"
     CDS             17577..18308
                     /gene="artJ"
                     /locus_tag="K0W68_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBI3073702.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18483..19610)
                     /gene="rlmC"
                     /locus_tag="K0W68_002150"
     CDS             complement(18483..19610)
                     /gene="rlmC"
                     /locus_tag="K0W68_002150"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBI3073703.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(19651..20139)
                     /locus_tag="K0W68_002151"
     CDS             complement(19651..20139)
                     /locus_tag="K0W68_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBI3073704.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(20199..21044)
                     /gene="potI"
                     /locus_tag="K0W68_002152"
     CDS             complement(20199..21044)
                     /gene="potI"
                     /locus_tag="K0W68_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBI3073705.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(21041..21994)
                     /gene="potH"
                     /locus_tag="K0W68_002153"
     CDS             complement(21041..21994)
                     /gene="potH"
                     /locus_tag="K0W68_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBI3073706.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(22004..23137)
                     /gene="potG"
                     /locus_tag="K0W68_002154"
     CDS             complement(22004..23137)
                     /gene="potG"
                     /locus_tag="K0W68_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBI3073707.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(23232..24344)
                     /gene="potF"
                     /locus_tag="K0W68_002155"
     CDS             complement(23232..24344)
                     /gene="potF"
                     /locus_tag="K0W68_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBI3073708.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(24695..25171)
                     /locus_tag="K0W68_002156"
     CDS             complement(24695..25171)
                     /locus_tag="K0W68_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBI3073709.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(25259..26161)
                     /gene="rimK"
                     /locus_tag="K0W68_002157"
     CDS             complement(25259..26161)
                     /gene="rimK"
                     /locus_tag="K0W68_002157"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBI3073710.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(26222..>26572)
                     /gene="nfsA"
                     /locus_tag="K0W68_002158"
     CDS             complement(26222..>26572)
                     /gene="nfsA"
                     /locus_tag="K0W68_002158"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBI3073711.1"
                     /translation="GGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKP
                     RLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRP
                     FILDYLHKQGWATR"
BASE COUNT         6555 a         6924 c         6570 g         6523 t
ORIGIN      
        1 cgctggtagt tgaactggta ttgagtgcag gtttcctgtt ggtgatccac ggcgcaaccg
       61 acaaattcgc gccggcaggt tttgcgccga tcgctattgg tctggcctta accctgattc
      121 acttaattag tattccggtg actaacactt ctgttaaccc ggcgcgcagc accgcggttg
      181 ctatcttcca gggcggctgg gcattagaac aactgtggtt cttctgggtg gtgccaattg
      241 tcggcggcat tatcggtggt ctgatttacc ggaccctgct ggaaaagcgt gattaattaa
      301 cgcagaaggc ctggcgtaat gtcaggcctt tttttaagat gttgttatat gttgattcat
      361 catcttgttg cgctcctttc atgagctaag ccacatattg ccactggcgc aaggagcgcg
      421 cagggggcgg ccaatcgccg ccgccccctg cccccccggg ctctggcgaa caaaatcgcc
      481 gctgcgcggt gccctcgact tatcccttac ggctaccggg tcgggcgcga ggtaacatcc
      541 ctgtaaaacg cgccctcagc ccacatccat gtgggctgcc ccggccttca gggaacgcct
      601 cggcgatttt gacgccacca acatcggtgc agtttgtaat taaagcgcaa gaatatcttt
      661 aaatgaaaac gaatactcta ctgctcctta tgcataacat ttccttttaa ttgcaacttt
      721 actcgtcctt ccgctttcag tagtgtgtca tcgcgcattt cgacttcatc tttgcaagga
      781 ctgggttttc atgttttctg ggctgttaat cattctggtt cccctgattg taggttacct
      841 cattccgctt cgccaacaag ctgcgttaaa agttattaat cagctattaa gctggatggt
      901 ttaccttatt ctctttttta tgggtatcag tctggcgttt ctcgataacc tcgccagtaa
      961 cctgttggcg attctgcatt attctgccgt cagtattacc gttattttac tgtgtaatat
     1021 tgccgccctg atgtggctgg agcgaggcct gccgtggcgc aaccaccatc agcaagaaaa
     1081 actcccgtcg cgtattgcga tggcgctgga gtcgctaaaa ctgtgcggcg tagtagtgat
     1141 tggttttgcc attggtctga gtggactggc tttcttacaa cacgcgaccg aagccagtga
     1201 atacacgtta attttgctac ttttcctcgt tggtattcag ttgcgcaata atggcatgac
     1261 cttaaagcag attgtcctta atcgccgggg aatgattgtc gccgtggtgg tggttgtcag
     1321 ttcattaatt ggtggtttaa ttaacgcctt tattcttgat ctccccatca ataccgcgct
     1381 ggcaatggcc tccggtttcg gctggtattc tctttccggt attttattga ccgaatcttt
     1441 tggtccggta atcgggagcg cggcgttttt taatgatctg gcccgtgaac tgattgctat
     1501 tatgttgatc cctgggctga ttcgccgcag ccgctctact gcactgggct tatgcggtgc
     1561 cacatcaatg gatttcaccc tgcccgttct tcaacgtact ggcgggctgg atatggtccc
     1621 ggcggcaatt gttcacggtt ttattcttag cctgttagtg ccgatcctca tcgccttttt
     1681 ctctgcgtaa tacctctctg gcggtagatc cctgccgcca aaattgcgct aaatcaatct
     1741 cccttaaagt tgcatgaaaa atccctttta tccccgcgtt aagcgtctta accttaaaca
     1801 tgtatattaa atataacttt aaaaggtgtg atcatgtttt gtgtgcaatg tgaacaaact
     1861 atccgtactc cggcaggaaa cggctgctca tacgcgcagg ggatgtgtgg taaaacggcg
     1921 gaaacttctg accttcagga tttactcatc gcggcgctgc aagggctttc tgcctgggcg
     1981 gtaaaagcgt gtgaatacgg catcatcaac cacgatgttg acagcttcgc gccacgtgcc
     2041 tttttctcaa ccctgaccaa cgttaacttc gattctccgc gtattgtcgg ctacgctcgt
     2101 gaagcgattg ccttgcgcga ggcgctgaaa gcacaatgcc tggctgtaga tgccaacgcg
     2161 cgcgtcgata acccgatggc tgacctgcaa ctggtgagcg atgatctcgg cgagctgcaa
     2221 cgtcaggcag cagaatttac ccctaacaaa gataaagcgg cgattggcga aaacattctc
     2281 ggcctgcgtc tgctgtgcct gtatggcctg aaaggtgcgg cggcctatat ggaacacgcg
     2341 catgttctcg gtcaatacga caacgatatt tatgcccagt accataaaat catggcgtgg
     2401 ctggggacct ggcctgccga tatgaacgcg cttcttgagt gttcaatgga aatcggccag
     2461 atgaacttca aagtgatgag cattctggat gcaggcgaaa ccggtaaata cggtcacccg
     2521 acgccaaccc aggtcaacgt caaagcgacg gcgggtaaat gcattctgat ttccggtcac
     2581 gatctcaaag atctctacaa cctgctggaa cagaccgaag gcacgggcgt taatgtctac
     2641 acccacggcg aaatgctgcc tgcgcatggc tacccggagc tgcgtaaatt caagcatctg
     2701 gtcggtaact acggcagcgg ctggcagaat cagcaagtgg agttcgctcg tttccctggc
     2761 cccatcgtga tgacctcgaa ctgcatcatc gacccaaccg taggcgctta tgacgatcgt
     2821 atctggaccc gcagcattgt tggctggcct ggcgtgcgtc atctggacgg tgatgatttc
     2881 tctgcggtta tcacccaggc gcaacagatg gcaggcttcc cgtacagcga aattccgcac
     2941 cttatcaccg tgggttttgg tcgccagacg ctgcttggcg ctgctgatac gctgattgat
     3001 ctggtgagcc gtgaaaaact gcgtcatatc ttcctgcttg gtggctgtga cggcgcacgc
     3061 ggcgagcgcc actacttcac cgatttcgcc accagcgtgc cggatgactg cctgatcctg
     3121 accctcgcct gtggtaaata tcgctttaac aaacttgagt ttggcgatat cgaaggtctg
     3181 ccgcgtctgg tagatgcagg tcaatgtaac gatgcttact cagcgattat tctggctgtc
     3241 actctggcag agaaactggg ctgtggtgtg aacgatctgc cgctgtcgct ggtgctctcc
     3301 tggtttgaac agaaagcgat cgtcattctg ctgacgctgc tttctctggg cgtgaaaaat
     3361 atcgtcaccg gcccgactgc acctggtttc ctgacaccag acctgctggc ggtgctgaac
     3421 gagaaattcg gcctgcgttc tatcaccact gttgaagaag acatgaagca actgttgagc
     3481 gcgtaaggag tttgtcgatg acgatgccaa cgaatcaatg cccgtggcgg atgcaggttc
     3541 atcacattac gcaagaaacg ccggatgtgt ggacgatttc cctgatttgc cacgattact
     3601 acccatatcg cgccgggcaa tatgcactgg tcagcgtgcg aaactcagcg gaaacgctgc
     3661 gtgcttacac catttcctcc acgccaggcg tgagtgaata tatcaccctg accgtgcggc
     3721 ggattgatga cggtgtcggc tcccagtggc tgacgcgcga tgtaaaacgc ggtgattatc
     3781 tctggctttc ggacgcgatg ggggaattta cctgcgacga taaagcagaa gataaattcc
     3841 tgttgctggc ggcaggctgc ggcgtcacgc cgattatgtc gatgcgtcgc tggcttgcga
     3901 agaaccgtcc acaggccgat gtgcgggtga tctacaacgt gcgtacgccg caggatgtta
     3961 ttttcgccga tgagtggcgt aactatccgg taacgctggt ggcagaaaat aacgttaccg
     4021 aaggctttat cgctggtcgt ctcactcgcg aactgctggc aggtgtacct gacttagctt
     4081 cacgtaccgt gatgacctgc ggcccggctc cgtatatgga ttgggtagag caggaagtga
     4141 aagcgctcgg cgtgacgcgt ttctttaaag agaaattctt cactccagta gcagaagcgg
     4201 cgaccagcgg tctgaaattc accaaactgc aaccggcacg agaattttac gccccggttg
     4261 gcaccacgct actggaggcg ctggaaagca ataacgttcc ggttgtcgct gcctgccgtg
     4321 cgggtgtttg cggctgctgt aagacaaaag tggtttccgg tgaatatacg gtgagcagta
     4381 caatgacgct gaccgacgcc gaaatcgctg aaggttacgt actggcctgc tcctgccatc
     4441 cgcaggggga tttggttctc gcataatcgc cttatgcccg atgatattcc tttcatcggg
     4501 ctatttaacc gttagtgcct cctttctctc ccatcccttc cccctccgtc agatgaacta
     4561 aacttgttac cgttatcaca ttcaggagat ggagaaccat gaaacaaacg gttgcagctt
     4621 atatcgccaa aacactcgaa tcggcagggg tgaaacgcat ctggggagtc acaggcgact
     4681 ctctgaacgg tcttagtgac agtcttaatc gcatgggcac catcgagtgg atgtccaccc
     4741 gccacgaaga agtggcggcc tttgccgctg gcgctgaagc acaacttagc ggagaactgg
     4801 cggtctgcgc cggatcgtgc ggccccggca acctgcactt aatcaacggc ctgttcgatt
     4861 gccaccgcaa tcacgttccg gtactggcga ttgccgctca tattccctcc agcgaaattg
     4921 gcagcggcta tttccaggaa acccacccac aagagctatt ccgcgaatgt agtcactatt
     4981 gcgagctggt ttccagcccg gagcagatcc cacaagtact ggcgattgcc atgcgcaaag
     5041 cggtgcttaa ccgtggcgtt tcggttgtcg tgttaccagg cgacgtggcg ttaaaacctg
     5101 cgccagaagg ggcaaccacg cactggtatc atgcgccaca accagtcgtg acgccggaag
     5161 aagaagagtt acgcaaactg gcgcaactgc tgcgttattc cagcaatatc gccctgatgt
     5221 gtggcagcgg ctgcgcgggg gcgcataaag agttagttga gtttgccggg aaaattaaag
     5281 cgcctattgt tcatgccctg cgcggtaaag aacatgtcga atacgataat ccgtatgatg
     5341 ttggaatgac cgggttaatc ggcttctcgt caggtttcca taccatgatg aacgccgaca
     5401 cgttagtgct actcggcacg caatttccct accgcgcctt ctacccgacc gatgccaaaa
     5461 tcattcagat tgatatcaac ccagccagca tcggcgctca cagcaaggtg gatatggcac
     5521 tggtcggcga tatcaagtcg actctgcgtg cattgcttcc attggtggaa gaaaaagccg
     5581 atcgcaagtt tctggataaa gcgctggaag attaccgcga cgcccgcaaa gggctggacg
     5641 atttagctaa accgagcgag aaagccattc acccgcaata tctggcgcag caaattagtc
     5701 attttgccgc cgatgacgct attttcacct gtgacgttgg tacgccaacg gtgtgggcgg
     5761 cacgttatct aaaaatgaac ggcaagcgtc gcctgttagg ttcgtttaac cacggttcga
     5821 tggctaacgc catgccgcag gcgctgggtg cgcaggcgac agagccagaa cgtcaggtgg
     5881 tcgccatgtg cggcgatggc ggttttagca tgttgatggg cgatttcctc tcagtagtgc
     5941 agatgaaact gccagtgaaa attgtcgtct ttaacaacag cgtgctgggc tttgtggcga
     6001 tggagatgaa agctggtggc tatttgactg acggcaccga actacacgac acaaactttg
     6061 cccgcattgc cgaagcgtgc ggcattacgg gtatccgtgt agaaaaagcg tctgaagttg
     6121 atgaagccct gcaacgcgcc ttctccatcg acggtccggt gctggtggat gtggtggtcg
     6181 ccaaagaaga gttagccatt ccaccgcaga tcaaactcga acaggccaaa ggatttagct
     6241 tgtatatgtt gcgcgcaatc atcagcggac gcggcgatga agtgatcgaa ctggcgaaaa
     6301 cgaactggct aaggtaaaaa gggtggcatt tcccgtcata ataaggacat gccatgattg
     6361 atttacgcag tgataccgtt acccgaccga gccgcgccat gctcgaagca atgatggccg
     6421 ccccggttgg ggacgacgtt tacggagacg accctaccgt taatgctctg caggactacg
     6481 ccgcagagct ttccggtaaa gaagccgcca tttttctgcc gaccggcacc caggctaacc
     6541 tggtcgctct gctcagtcac tgcgaacgcg gcgaagagta tattgtcggt caggccgcgc
     6601 ataattatct gtttgaagcc ggtggcgcag cggtgctggg cagtattcaa ccgcaaccca
     6661 tcgacgcggc tgccgacggc acgctaccgc tggataaagt ggcgatgaaa atcaaacccg
     6721 acgatatcca tttcgcccgc accaaattac tcagtctaga aaacacccac aacggcaaag
     6781 tgctgccgcg tgaatacctg aaagaagcat gggaatttac ccgcaagcgc aatctggcgc
     6841 tgcatgttga cggtgcgcgc atctttaatg ccgtggtggc ttacggctgc gaactgaaag
     6901 agatcacgca atattgtgat tcgttcacca tttgcctgtc gaaaggtctt gggacgccag
     6961 tcggttcatt actcgtcggt aatcgtgatt acattaaacg tgccatccgc tggcggaaaa
     7021 tggccggtgg cgggatgcgt cagtccggga ttctggctgc cgccgggatg tatgccctga
     7081 aaaataacgt cgcacggttg caggaagatc acgacaacgc cgcctggatg gcggagcaac
     7141 tgcgtgaagc aggcgcggat gtgatgcgtc aggacactaa tatgctgttt gttcgcgtcg
     7201 gggaagaaaa tgctgccgcg ttaggcgaat acatgaaagc gagaaacgtg ctgattaacg
     7261 cctcgccgat tgtccgcctg gtgacgcatc ttgacgtctc gcgcgaacaa ctggcggaag
     7321 tcgccgccca ctggcgcgca ttcctggcgc gttaaggaga gagacgtgcc gcaacgcatt
     7381 ttagttctcg gtgccagtgg ctacattggt cagcatctgg tgcgcacact cagccagcaa
     7441 gggcatcaga tcctggcggc ggcacgtcat gtcgacaggc ttgcaaagct gcaactggca
     7501 aacgtcagtt gccataaagt cgatctcagc tggccggata accttccggc cctgttgcag
     7561 gatatcgata ccgtctattt tctggtgcac agcatgggcg aaggcggcga ttttatcgct
     7621 caggagcgcc aggtggctct caacgtccgc gatgcgctac gtgaagtacc agttaagcaa
     7681 ttaatctttc tcagttcgtt gcaggccccg ccacatgagc agtcggatca tctgcgtgct
     7741 cgtcaggcta cggcggacat tcttcgtgaa gcgaatgtac ctgtgaccga acttcgggcc
     7801 ggaattatcg ttggcgcagg ttcagcggcg ttcgaagtca tgcgcgatat ggtctacaac
     7861 ctgccagtgt taacgccgcc acgctgggta cgttcacgca ccacgcccat cgcgctggaa
     7921 aacttgctgc actatctggt ggcgttgtta gatcatccgg ccagcgaaca ccgcatcttc
     7981 gaagccgccg gaccagaggt gctcagttat cagcaacagt ttgaacattt tatggcggtg
     8041 agcggtaagc gccgctggtt gatccccatc cccctcccca cccgctggat ttcggtgtgg
     8101 tttctcaatg tgattacttc cgtaccgccc accaccgcca gggcgttgat tcaggggctg
     8161 aaacacgatc tgctggcgga tgataccgcg ctacgtgcac tcatcccaca acggctgatc
     8221 gctttcgatg acgcggtacg tagcacgttg aaagaggagg aaaaactggt caactccagc
     8281 gactggggct acgacgctca ggcctttgcc cgctggcgac cggagtacgg ttattttgcc
     8341 aaacaggcgg ggtttaccgt taaaacgtcc gccagccttg ctgctttatg gcaggtagtg
     8401 aaccaaatcg gcggtaaaga gcgttatttc tttggcaata ttttgtggca gacacgggcg
     8461 ttgatggacc gcgcgatcgg tcataagctg gcgaaaggcc gcccggagcg cgaatattta
     8521 cagactggcg atgcggtgga tagctggaaa gtgattgtcg ttgaaccgga aaaacaactt
     8581 acgttgttat ttggcatgaa agcgtcgggg ctgggacgac tgtgttttag cctggaagat
     8641 aaaggcgact atcgtactat cgatgtccgc gctttctggc atccgcacgg tatgccgggg
     8701 ctgttttact ggttattgat gatccccgcg catctgttta tttttcgcgg aatggcaaaa
     8761 caaatcgcca gactggcaga acaaagcaca gattaatcga caaaacctgc gttattcttt
     8821 catgtttccc attgcatcat gggggaaatc acggaagaat gcgcacgtca ttttctcaac
     8881 aacagtggat tgatatgaag gtactggtta ccggcgccac cagcggctta ggtcgaaacg
     8941 cggtagagtt tttatgccag aaaggcatca gcgtgcgagc gaccggtcgc aacgaggcaa
     9001 tgggcaaatt gctggaaaaa atgggcgcag agtttgttcc ggcggatctg accgagctgg
     9061 tttcatcaca agctaaagtg atgctcgcgg gcattgatac gctgtggcac tgctccagct
     9121 ttacctcacc ctgggggaca caacaggctt tcgatctggc taacgttcgc gccactcgcc
     9181 gcctgggtga atgggctgtc gcctggggtg tgcgtaactt tattcatatc tcttccccct
     9241 ccctgtactt cgattatcac caccatcgcg atattaaaga agattttcgc cctcaccgct
     9301 tcgccaacga gtttgcccgc agcaaagcag ccagcgaaga agtgatcaat atgctttcgc
     9361 aggcgaatcc acaaacgcgc tttactattc tgcgcccaca aagtctgttc ggaccgcacg
     9421 ataaagtctt tattccccgt ctggcgcata tgatgcacca ctacggcagt attctgttac
     9481 cgcatggcgg cagtgcgctg gtcgatatga cctactatga aaataccgtg cacgcaatgt
     9541 ggctggcaag ccaggaagcc tgcgataagc taccttccgg gcgtgtgtac aatatcacca
     9601 acggcgagca tcgcacactg cgcagcatcg tgcagaagct gatcgacgag ttgaatattg
     9661 actgtcgtat tcgttccgtc ccctacccga tgctggatat gatcgcccgc agcatggagc
     9721 gtttaggccg caagtcagca aaagagccgc cgctgaccca ctacggcgtc tccaagttga
     9781 attttgactt tacgctggat attacgcgtg cgcaggaaga gttaggttat cagccgatca
     9841 tcaccctaga tgaaggtatc gagaaaactg ccgcctggct acgcgaccac ggaaaactgc
     9901 cgcgctaatc ctgcccgtat ttctccagca atgcttcggc aatcgcctga gtttctgcat
     9961 ccgcttcgcc gttatataac gtcgggcgga aatgcatctg gaatgccata atcacgcgcc
    10021 gctgctcgcg cggtgtcata tcaggtttaa cgtcataacc gtagcgcgcc aaaagctcca
    10081 gcaatgacgc ggtatctacc ggtgtgtgcg gcgcgcgccc ggcaaggtaa aagttaaccc
    10141 gctgcgcatc cggccaggca ccaatcccct gctgcgccag ttgctgccag ggaaataatg
    10201 gccccggatc gtctttgcgc tgcggtgcga tatccgcatg tgccactacg ttttccggct
    10261 tgatgtgata acgggcaata atatctttcg ccagtggaat aagcgcctga atctgtgtcg
    10321 gttcaaacgg ggcaaaatat ttcactccgg ccgatttttg ccagccacgg ttttccagct
    10381 caatgccaat agaggtgtcg ttaaggcgcg ttgccccgcg ccaggcgcta atccccgcat
    10441 gccaggccag ttcttgttcc ggcaccagtt gccagatgcg cggtttaccg ttgtatcgcg
    10501 gtggtaccgc agggaccaga taatgcgagc tgacctgctt atcggtcagt gtcgccagcg
    10561 agctatcaaa atcatctgcg gtgtagtgga tcaccagcac tttaatgcgc ggatacgccg
    10621 cctgcgcctg gcgtcgggta tcaagctgat atccctcttt ctcgacaatg cctttttcgc
    10681 ctgcacaccc tgccaataac agagcggcag cgaccagcca gaagactctt ctcatcgacg
    10741 cgttttcacc gccgtaccgc tgacgctaac catcagcata ctgccgtttt gcccgaccgt
    10801 ttcgtagtcg atatcaatac cgacgacggc atcggccccc agcgcccgcg cctgggagcc
    10861 taattcctca aaggcgatct cccgtgcttt acgcagttct ttttcatacg caccggaacg
    10921 tccgccaacg atatcgcgga taccggcaaa gaaatcacgg aaaatattgg cacctaaaat
    10981 cgcttcgccg gtcaccacac cgcaatattc aacgatggtc tggccttcca gagttggggt
    11041 tgtagaaaat tgcatgatcg atctccttgt ttgttaagcc ttcgatctct aaagcattat
    11101 cgaactgata tgccatgttg ggaagaaggt tttgatcagt ttaggaaatc atctcattgt
    11161 gtcaaaaaca cccacttaag tctttacgat tatccgttgc ataacccgat attggtttca
    11221 ttatagggta ccgcttacct gtcttattat gttgcaccaa caacattaat gagcggaaag
    11281 aggcaactta tattgtaaat gactcctaaa gtcttcccca tcttgtgact gaggtatata
    11341 cctctggatt gctttttggt cagacgccgc aaaacaagaa agtaataaga tctcaatgct
    11401 tttgtcatta atataagcga agatcattct tgcaaattcg taaataaacg cccgttcctg
    11461 agcgcttaat agtttaatgc aatatttatt cttaagagag aaaataattt caaggaaagc
    11521 atccgcagca accttcacgt tattcccggc gctcatatta tatatatccc ttgcacagat
    11581 agagtacaga gatagtaaac agccatacaa ataatcattt attaactctt ttgtccattt
    11641 tactggcaac catggtttta tcttgacacc atgcatacta cagtcatgaa aaagacattt
    11701 gctgaaatta actcttatca tatcaacatt aaaaaaatgg caattaataa attttaaaga
    11761 atgagtattt acgttgttaa ttttaacaga tctaaacgaa acatttttga atacattatt
    11821 tattatattg ctatttgtta ttttaacatc acctaaactg ccgttttcaa aacaacattt
    11881 tttcaatatg ctttcattca caacagtgtg tgataagtag agattactta tgttttgctt
    11941 tattaatgcc acgtcattaa aatgactata tttaatgaaa aaaccatcat tgagtagtgt
    12001 tttaagattg actaaattac ttgcatcttc acgtaccatc gtggcaactg gaacaggcag
    12061 agacatgaaa acttcttcat tcaccatcaa gtgaatcatg cattgcgttc cctttgtcat
    12121 atccacacat acgctacaaa tatcgtggta atgcgaagag cgcatttcag gaaacagatt
    12181 aaacacggta tcttgaaagg cactaaatcg tgttaataaa tcctgataat gaacaggttt
    12241 tgtttcaaaa ttatcatcag caaatccaag cactattgta tgctctacat ctaacgcagt
    12301 ttcaaatggc tcttcttctg taaaatcatt atttaaaacc tcacctgacg tattatcact
    12361 gtcataaaaa gtgttcatca atgcgatatt gtttgttaca gcgtcttttt cgatattctc
    12421 actaattgca tatcgcctgc tataatcata aaaatgaata aacgcgccct ttataaagga
    12481 tatatattgt tctttgttaa agtcaatacg gctgtataat ttttcaaaaa tcacattcgc
    12541 aattttacta ccaatggtat gcctgtataa taatgggtgc gattcaatcg taaaatgctt
    12601 gtctaattga tcttgtaatt tattctcata gtccagaggg aaaagaaact gttgtctttc
    12661 aagtttccca ttcgatacat tacgtattcc aatagagatt ctttcattac ccgcctttat
    12721 ttcaacttcg ttatcttctg atttacccag atatttttta acatcagtct cgaaagcatt
    12781 actaaaaaaa gtattaaatt ggtaattcgc tgatggcata ttttccgcga gcaacacatc
    12841 attttttatg agatcactgt cagcatttat tgctaatatc agatgcaagt agatggtatc
    12901 ccatgtagac aactcaccct tacgactgga atttaatatc gcgaaaaatt ccctcagtga
    12961 atccaggaaa ttatcacttt gatttagttg tgtgcctgga aatgatgatg cttgatcaga
    13021 tatattgttt atcatattaa aaatcaccca agaaaaacga taatccaccg aactcctcgt
    13081 gctttataat tataaaagca caacgccttt tatattcata ttaattaacg agatactgaa
    13141 acaacttaat ttattcttcg ctaaaattca ttagcttaaa ttctttagcc acaattgtag
    13201 cataaataat attatcgaga gtcaaagctg ggactatcaa atttcgctca ttttcaaaaa
    13261 tacaacgtaa ttttggcaat catcttaatc gcaggatctc ttatcgcatg tgaaaaattt
    13321 tgtaatgatt agacaataac cgcgttcatt tatcacgcgt tataaatgtg aaactggctt
    13381 cttgcagatg ctcttaacct ctacaggagg atttatacca ccatttcaga actatcatat
    13441 ttaccttaaa tgatcatttc agccatataa cacgctcaac ctcacaaatg gtttacactg
    13501 tctattgttt gggtaattta agtaagtcag ctacgagcaa ccttgagtgg atagccagca
    13561 atggactcaa acccaatcag gtgttcagat tgtttcagaa ttgtattaaa aacaatgtat
    13621 taaataatct gatagcgttt ggtcgtagtg tgacgcagac agcaagctaa taaggaaatg
    13681 attatgcgct accgttcact atctctactt attccatgtg cactgctgct gagtgcctgt
    13741 accaccgtca cgccagctta taaagataac ggtccccgca ccggttcctg tgtccagggg
    13801 gggcctgaca gcgtggctca acaattctat gactaccgca ttcaacatcg cagcaatgac
    13861 attaccgccc tgcgccccta tttgagtgac aaactggcaa cccttcttag cgatgccagc
    13921 cgcgataata gtcatcgtga attgttaagc agcgatccgt tctccagtcg aaccaccttg
    13981 ccagatagcg cccacgttgc cagtgcatcg actatcccta atcgtgatgc ccgtaacatt
    14041 ccattgcgcg tagatctcaa acagggtgac cagggttggc aggatgaagt gttaatgatt
    14101 caggaaggcc agtgttgggt catcgacgat gtgcgctact taggcggcag cgttcatgcc
    14161 acggcgggta cgctccgtca gtccatcgag aatcgctaac gccttgatca accaataaac
    14221 cccgtaaatt gtccggcatt tcgtgaggaa tgccgacatt tatgctcgcc gaccaccgcc
    14281 cccgttattt tgtgctatgt ttattgaata atgcgctttg cttttaactt ttaaagcaga
    14341 aatattgcat aattattctg tcaaaggtac tatcttcgac ctcaatagct atcagactgc
    14401 cagtatacga gtgtcaatga gtattcaatt aaacggcatt aattgcttct acggcgcgca
    14461 tcaggcgctg ttcgatatca cgctggattg cccacagggc gaaacgctgg tgttacttgg
    14521 ccccagcggc gcgggtaaaa gctcgctgct gcgtgtactc aatctgcttg agatgccacg
    14581 ctccggtacg ctcaacattg caggcaacca tttcgatttc accaaaacac cctctgacaa
    14641 agcgattcgc gatttgcgtc gtaacgttgg catggtgttt cagcaataca acctgtggcc
    14701 gcatctgacc gtgcagcaaa acctgattga agcgccctgc cgtgtactgg ggttgagtaa
    14761 agatcaggcg ctggcccgtg cagaaaaact gctggaacgt ctgcgtctca aaccttatag
    14821 cgatcgttac ccgctgcatc tttctggtgg tcagcagcag cgtgttgcta ttgcccgtgc
    14881 gttgatgatg gaaccgcagg tactgctgtt cgatgaaccg accgccgcac tggacccgga
    14941 aattacggca caaatcgtca gcatcattcg tgagctggca gaaacgaata ttacccaggt
    15001 gatcgtcacc cacgaagttg aagtggcgcg taaaaccgcc agccgagtgg tgtatatgga
    15061 aaatggtcat atcgtagaac aaggcgacgc gagctgcttt accgagccgc aaaccgaagc
    15121 atttaaaaac tatctctctc actaagattc gggataacaa caatgaaaaa agttctgatt
    15181 gccgcgttaa ttgcaggttt tagtctttcc gccacagctg ccgaaaccat tcgttttgct
    15241 accgaagcat cctatcctcc gtttgaatcg attgatgcaa acaaccagat cgttggtttt
    15301 gacgtcgacc tggcacaagc gctgtgtaaa gagattgatg caacctgcac tttctctaac
    15361 caggcgtttg acagcctgat cccaagcctg aaattccgtc gcgtagaagc cgtgatggcg
    15421 ggcatggata tcactccgga gcgtgaaaag caggtgctgt ttaccacccc gtactatgac
    15481 aactctgccc tgtttgtggg tcagcaaggc aaatacacca gtgttgatca gctgaaaggc
    15541 aaaaaagtcg gcgtacagaa cgggacgaca caccagaaat tcattatgga taagcacccg
    15601 gaaatcacta ccgttccgta tgacagctac cagaacgcaa aactggatct gcaaaacggg
    15661 cgtatcgacg gcgtcttcgg tgacaccgca gtggtcactg agtggctgaa agataacccg
    15721 aaactggcgg cggtgggcga caaagtgacc gataaagatt acttcggcac tggcctcggc
    15781 atcgcggtac gtcagggcaa cactgagctg cagcagaaac tcaacactgc gctggaaaaa
    15841 gtgaagaaag atggcactta cgaaaccatc tacaacaaat ggttccagaa gtaattcctg
    15901 atgaatgaat tttttccttt agcaagcgcc gccgggatga ccgtcggcct tgccgtttgt
    15961 gcattgattg tcgggctggc gctggcgatg ttctttgcgg tatgggagtc ggcaaaatgg
    16021 cgtcctgtcg cgtgggcagg ttcagcgctg gtaaccattc tgcgtggcct gccagaaatt
    16081 ctggtggtgc tgtttatcta ttttggctcc tcgcagctgc tgctgacgct ttcggatggc
    16141 ttcactatca atcttgggtt cgtgcagatc ccagtgcaga tggacattga gaacttcgac
    16201 gttagcccgt tcctttgtgg tgtcatcgct ctgtcactgc tgtatgccgc ctatgcctcg
    16261 caaacgcttc ggggcgcgtt gaaagcggtg ccggtgggtc agtgggagtc tggtcaggcg
    16321 ctggggctgt cgaaatcggc tatctttttc cgtctggtga tgccgcagat gtggcgtcat
    16381 gcgctgcctg gcctcggtaa ccagtggctg gtgctgctga aagataccgc gctggtcagt
    16441 ttgattagtg tgaatgattt aatgctgcaa acaaaaagca tcgctactcg tacccaggaa
    16501 ccatttacct ggtacattgt ggcggcggcg atttacctgg tgatcaccct gctcagtcag
    16561 tacattctca aacgcattga cctgcgcgcg acacgttttg agcggaggcc cagctaatgt
    16621 ttgagtattt acccgaactg atgaaagggc tacacaccag cctgacgcta accgttgcct
    16681 cgctgattgt ggcactgatt ctggcattga tttttaccat catcctgacg ctgaaaacgc
    16741 cggtgctggt gtggctggtg cggggttata tcacgctgtt taccggtacg ccgctgctgg
    16801 tgcagatctt cctgatttat tacgggccgg gccagtttcc gacgttgcag gagtatccgg
    16861 cactgtggca tttgttgtca gaaccgtggt tatgtgcgct gattgcgtta tcgctgaaca
    16921 gtgcggcgta taccacgcag ctgttttacg gtgcaattcg tgcgatccca gaaggtcagt
    16981 ggcagtcctg tagcgccctg ggaatgagca aaaaagatac gctggcgctc ctgctgccgt
    17041 atgcctttaa acgctcgctc tcttcttatt ccaacgaagt ggtgctggtg ttcaaaagta
    17101 cctctctggc ttacaccatt acgctgatgg aagtgatggg atacagccag ttgttgtacg
    17161 gacgcaccta cgatgtaatg gtgttcggtg cggcagggat tatttacctg gtcgtcaacg
    17221 gcctgctgac gctgatgatg cgtctgatcg agcgcaaagc actggcattc gaacggcgga
    17281 attaatgtag ctgcgtacat tcgccggggg ggtttgtagg tctgataaga cgcgccagcg
    17341 tcgcatcaga catcagcacg gtgcctgtgc cggatgcggc gtggacgcct tatccggcct
    17401 acatagtgca taaattctta taatgaagac ggacaactca ctaagttatc cgtctttttt
    17461 tatttcattt aaattattta atcatattta ttgcatataa attcacttga tggcattgtt
    17521 atcccatgcc gcagacacgg ccaaaaatca taagattgac agacgggagt tccatcatga
    17581 aaaagttagt tcttgccgct ttacttgctt cctttacttt cggtgcttct gccgcagaga
    17641 aaatcaattt tggcgtttca gccacctatc caccctttga atctataggt gctaataatg
    17701 agattgtcgg ctttgatatc gatctggcaa aagccttgtg caaacaaatg caggcagaat
    17761 gtacttttac taatcacgcg ttcgacagcc tgatcccgtc actgaaattc agaaaatatg
    17821 actccgtaat ctccggtatg gatatcacgc ccgagcgtag caagcaggta tcgtttacca
    17881 cgccctacta tgaaaactca gccgtcgtga ttgccaaaaa agatacctac aaaacgtttg
    17941 ccgatctgaa aggcaaacgt attgggatgg aaaacggcac cacgcaccag aaatatattc
    18001 aggatcagca ccccgaagtg aaaactgtct cctatgacag ttatcagaat gcctttatcg
    18061 atctgaaaaa tggtcgtatt gatggggtat ttggtgacac agcggtggta aacgaatggc
    18121 tgaaaaccaa tccgcaactg ggcgttgcta ctgaaaaagt gaccgatccg caatacttcg
    18181 gcaccggtct gggcatcgct gtacgtccgg ataacaaagc tctgctggaa aaactgaata
    18241 acgcgctggc agcaattaaa gctgacggta catatcagaa aatcaatgac cagtggttcc
    18301 cgcagtaatt gagactgatg acaaaagtaa gattgcctga tgcgctgcgc ttatctggcc
    18361 cacatcaact ctgcaatgta ttgatttagc atgattttgt aggccggata aggcgtacac
    18421 gccgcatccg gcatgaacaa agcgcacctg atcagcaatc tgaagccgca cctgcggctt
    18481 ttttattgct tcaccagcag cgtcatcact tcatagtgcg cggtgtgcgg gaacatatca
    18541 aaaagctgta cccgttcgat gcggtaacct ggcagttcgc ggatatcttt cgccatggtt
    18601 tgggcgttac agctggagta gataataaaa cgcggtgcca tcgttgagag ataatcacac
    18661 agcggtttac caatgccgcg gcgcggcggg ttaaccagca ccagctccgg cacatccccc
    18721 tgagcggtgg caaactgagt ggagtccagc gcctgaaatt gcaaacgcgt taagcccagt
    18781 tcagcggctg actgctttgc acaggcaatg gcctctgatg caatttcgat cccggttaac
    18841 tgcatgtcag gcgtcgcgca gtgtaaacca aagccaccca caccgcagaa cagatcccac
    18901 atatgtttaa ctggcagctg ccgtacccag tcgcgcgcgg tagcgtacag ctggctggcg
    18961 accgccggat tagtctggaa gaaactttgc ggacggatcc acagcggtac gtcattaaaa
    19021 cgctctgcca gcgcctgttg ttcagtcagg tagatctccg tctccccttc cataatcgcc
    19081 atatgtaccg gctgaatatt gacggtaata actttcagtt gcggtagttg ttcctgtaac
    19141 cacggcagcg ccttacgcag ttgtgccagt ttggtttcag aacgcagtac aaagcgcagc
    19201 atcatgccgc catcgctctg gctttcagtc agcagaatgt atttcagttc gccacgctta
    19261 cgcgccacgt tgtagggcgt taaccccgca cgggcgataa acggttttag cgccgcaaaa
    19321 acgggcgcaa aagaggcagg ataaagcggg cagtcacaaa ggtcttccgg cgtgccatcg
    19381 cgatgcagca taccgagcag tggtttttca acgctaccac tcaccaccat tttggcttta
    19441 ttacgaaacc cttgttccgg gcctgacacc ggcgcgcacc attcctcaac cggaaaatcg
    19501 gcgagcagat ttttaagatc ggcggtttta gcggagagtt gctctggaat cggctgcgtt
    19561 atccactgac aggaacgaca gcgacccgcg tcgtaaagtg cgcactgcat acattgacct
    19621 tcacatcatc aggggcgacg attatacaca ttattgcaac tggaagaacc gccgactggt
    19681 cgagggaacg aacagcagca tcaggatgag catatccggc agcttctgca gcatcaggct
    19741 atggaagatt tcacgttttg attcaccggg aatgctgaac agctccggat aaccgtaccc
    19801 cagcgaagcc gcccacaagt aacttgcggc ggtgatttgt gtcagcagat atagccagcg
    19861 cgcccagcga cgccctttca ccagtgaaaa cgcacaccag atctcaatga aaaccagcac
    19921 cagactgctt aaaaagacca gcgttaaact ccaggtttgt acgctgcgat gaatgaactc
    19981 gccaataccg cgcacgccca gcgtattgaa aatcatcagc acgtcgaggc cacggatcat
    20041 aataatggcg agcgccgcca cctgcaccag cgcaggcaca ttcaggcgag catgagatga
    20101 agatgttttc ttaaaaaatc ccaacgtttc gtcttccatg aaaacaatgc cgcggcatgc
    20161 gcggcattat gtagccaggt tggcaaattt tagtgtcttc agccatgtct tgcacgctgg
    20221 atatcgcgta tccgctgttt ttctgcgcga gccatcagat accaggcgat aaatccgaca
    20281 attccgaccg caccgagaat taacgttgca agggcgttga tttccggatt cactcccatc
    20341 cgcacgctgg aaaagaccag catcggtaac gtggtggctc ccggcccgga aacaaagctg
    20401 gcgatcacca gatcatcaag cgacaaagta aaagccagta accagccaga aatgatcgcg
    20461 ggcatgatca tcggtagcgt aatgacgaaa aacactttca gcggcgtcgc accgagatcc
    20521 atcgctgctt cttctatcga gcgatccagt tcccgcaatc ttgaagaaat aacgaccgcc
    20581 acataagccg tacaaaaagt gacatgcgcc agccagatgg tgagcatacc gcggtccgca
    20641 ggccagccaa tggcatgagc aagcgcgacg aataataaca acagcgacaa gcccgtgatg
    20701 ccatctggca tcaccagcgg cgcggtgatc ataaaggcaa aaccatttga tccgcgaaac
    20761 ctgccaaaac gcaccagcac caccgccgca atagtcccga ggatcgccgc cgccgttgcc
    20821 gcacaggcag caattgtcag gcttaaacca accgcactca tcatcgcgtc atcgcgcaat
    20881 aactcaccat accagcgcgt tgaccagccg gcccacaccg tcaccagctt cgagctgtta
    20941 aacgaataga taaccagcat cagcattggc gcgtagagaa aggtaaagcc cagcagcaaa
    21001 atcacaatcc gccagggcga acgaactacc ggtaaattat tcatccgtgt tctcccacgc
    21061 ttttttgctg gtgtttgtgg aaccacatta tcggcacaat tagcagcagc aacatgatga
    21121 tcgctaccgc cgaggccacc ggccagtcgc ggttattgaa aaactcttgc cataacacgc
    21181 gcccgatcat gatgctgtcc gggccaccga gcagttccgg gatcacaaac tcgcccacag
    21241 ccgggataaa caccagcatc gatccggcaa taatcccacc tttagtcagc ggcacgatca
    21301 cagtaaagaa cgttttcagc ggtcgtgcac cgagatccag cgctgcttcc accagcgaat
    21361 aatcaatacg aatcaacgcg gtataaatcg gcagtaccat aaacggcacg taagcgtaaa
    21421 caatgccgat ataaacggcc agattggtat gcagaatggt cagcggttga tcgataaccc
    21481 ccagccacag cagaaaatta ttcagcacac cgttgttttt taatattccc atccaggcat
    21541 aaacgcggat cagaaacgag gtccacgacg gcaggatcac cagtagtaat aaaatattac
    21601 gggtcgaagg cttactgtgc gccaccgccc acgccagcgg atagccgatc agtaaacagc
    21661 aaaatgtcga aatcgccgcc acctgtaacg actggagata agcatcaaaa tagagcggat
    21721 cgtcggtcag ttgcagaaaa ttaccgagat taagagtgat ggaaagttgc ccgtcagccc
    21781 actccatcag ttcggtataa ggtggaatag cgcgcgccat ctctcccagg cttattttaa
    21841 agacaatcag aaatggcagc agaaacagca agatcaacca gatatatagc aacgcaatga
    21901 ccagtttgcg cccatgcttc atttgcagct gcgacaacca cagcttaaat ccgcccggcg
    21961 gtttcgactg ggcagcaggt tcaagtgtac tcatctttgc tccttaaacc gtcagcacca
    22021 cacagctgtc cacttcccag cacaaacgca cttcgtcgcc ccaggtcggt aaccctttac
    22081 gatgacgatg ggcgttttgt agctgggcgc tgatcatctg cccacttttc agtcgaacgt
    22141 gatacaccga aagatcgccg agataggcaa tgtgtatcac ctcccccacc gcgaagttac
    22201 aaccattggc gggcggctct tcgcaaagca tgattttttc cgggcgcagc gctacgtgta
    22261 ccggcacgtt atcgaccact gaagcatccg catcgacttt cagtggatgc accagccccg
    22321 gcgaatcaag caccaggcca tcttcctgac gctctttgag tacaccttca aagacattaa
    22381 ccgaaccgat aaattcagcg ctgtagcggg tagttggatg ctcgtagatc tcttccggct
    22441 cgccaatctg gacaaatttc ccgcgattca taatggcgat acgccccgcc atggtcatcg
    22501 cctcttcctg atcgtgggtg accatcacac aagtcacacc gacgcgctcc agaatatcca
    22561 ccacttcaag ctgcatccgg tcacgcagct ttttatccag cgcgcccatc ggctcatcga
    22621 gcagtaatag tttcgggcgt ttggcaaggc ttcgggccag ggccacacgc tgtcgctgac
    22681 caccggaaag ctgatgcggt ttgcgtttgg cgaactcctg catgtgcacc aggccgagca
    22741 tctcatggac ccgactggca atttccgctt tcggtagttt gtcctgtttc aggccaaaag
    22801 cgatgttctg ttccacggtc atatgcggaa acagcgcgta agactgaaac atcatattga
    22861 tggggcgcag gtaaggcggc acctgtgaca aatcgacgcc atcgagcatt atctgcccgg
    22921 cagaaggttg ttcaaaacct gccagcatac gcagcagcgt ggacttgcca cagccggatg
    22981 cgcccagcag cgcgaagatt tcacctttgt agatggtcag gctgacatca tcgaccgcat
    23041 gctgaccatc gtaggatttg gtgaggttgc ggatttctaa tagcggcgtc agcgccttac
    23101 gggttttcgc ctgcgggcga gggatagcgt cattcacggc tgctctccgg cattgaatgt
    23161 gtgcaagcgc accatgatgg tgcgaattgc cggctggtgt ggtgcgcccg cccggcatct
    23221 aaggctgcgg attattttcc gctcttcact ttggtccacg cgcgggtgcg cacacggtcg
    23281 attttcggat cctgtacttt cagagtgaat agcttcgcac gaacgtccgc cggaggataa
    23341 atacccgggt tctcacggac ttccgcactc accagcggcg tggctgcttt attggcgttg
    23401 gcatagaaca catggtcgga aatatgcgct actacatccg ggcgcagcag gtaattcagg
    23461 aactgatagg cttcgtcttt gtttttggca tccgcaggca tggcgaatac atcaaagaac
    23521 gccatcgccc cttcttttgg aatcgagaac gagacattca ctccattctt cgcttccttc
    23581 gcgcggtttg acgcctgcca gacatcacct gcccagccga tagcgacgca aatatcgccg
    23641 tttgccaggt cgttaatgta ttgagatgaa tggaaataac gaatgttcgg gcgcagcttt
    23701 aacagcagat cggttgccgg tccggtgtaa tcatccgctt tagtgctgtt gggatctttg
    23761 ccgagataat tcaacacggt agcaaaaact tcttctggcg catccaggaa agagacaccg
    23821 cagcttttca gtttttccag attttcaggt ttgaggatca agtcccagct atcgacgggc
    23881 gcgttttcgc ccagcacagc tttaacttta tcaacgttat agccaatccc ggtcgtcgcc
    23941 cacatatagg gcatagcaaa tttattgtcg ggatcgtgtt tggcgaccag cttcagcagc
    24001 tccgggtcga gattcttcca ctccggcaat ttgcttttgt ccagcggctg gaaaactccc
    24061 gcagtcaact ggcgttcaag aaagctggca gatggaacca ccaagtcaaa gccggtgcta
    24121 cccgccatta atttgccttc cagtacttcg ttagagtcga aaacatcgta gacgacttta
    24181 ataccggttt ctttttcaaa attggccacc gtgtccgggg cgatataatc agaccagtta
    24241 taaatgtgga gtgttttttg ttcagccgcg agcgtgccga cagagacggc catcagagca
    24301 cccgcagcca gacccgaaag ccatttttta tttaaggcgg tcatatctct ttccttctga
    24361 aagttcgtta acaaacgaac aaaagttgtc acactaaaag atatgcaata aacgtgcata
    24421 ttttttatcg gcggggtgta agaaaagaga actctctccc ggccagcact gctcgctata
    24481 taaaagcatc gcaagaataa ctttagccag ggtttgagac tatagctcgg attaaaaaac
    24541 ggtatttaac aaatttttat gcgtatttgc tctatgtgat aaagccaaat cgacacaaga
    24601 gaagtgaaat aatctttaag aaaaggttaa aaacagcaga taattttgca gcgcgcagcc
    24661 actgcaccag cgactgcgtg ctcgaaagga gttatcagtg gaggaaatag ttttcggtaa
    24721 cgttgttttg cccttcatcg tcagtcggta acagcagttg atgggcatta gcttcaagaa
    24781 tgaccatcga aatctgctct tcgctctgac gcacgaacca tgcaaactgc tcataggtta
    24841 cgccctgcat aacggataaa gactggcaaa ccaccagctt tggcagatta tcatcctgca
    24901 tatcaagaaa tgctttcacg gtcagcgaac tggcattgat ggcagataaa tccgccgcca
    24961 gcggcaatac ggctgaaggt cggacttccg ccatggcaga aaacaggatc gtgttatcga
    25021 tcagatcgat tttggcatca aagacaccgt cgaaattctg catatggggc agatgcagag
    25081 cctgacagtt atcacattca aaaaaactca tccccaggtc atcgagccat tgacgcagcg
    25141 tatccagacc aggaacgacc agcgatgtca taattttgta caacctcttt cgataaaaag
    25201 accggcacag cttacgcaaa aagcgcaggc aaaaccatga tcagtaatgt gattgcgact
    25261 aaccacccgt tttcaggcaa tattctgtcg tagcgtggcg ttcgatccag cggatcattt
    25321 tacccgcgat gtcgatacca gtggtttttt ctattccttc cagccccggc gacgcattca
    25381 cttccatcac caacggcccg cgattagcac gcagaatatc cacaccagca acgtccagcg
    25441 ccatcgttcg cgcggctttt atcgcgattt cacgctcctg tggtgtgata ctggcgacgc
    25501 ttgccgcgcc gccacgatgc aaattggaac gaaaatcgcc ctctttcgcc cgccgttcaa
    25561 tcgcagcgac cacttcatcg ccaacaacca gacagcggat atcgcaccct tgcgcctctt
    25621 tgatatattc ctgcaccaga atatgcgcgt tcagaccgcg gaaagcgtca atcacgcttt
    25681 ccgccgcctg acgcgtctcc gccagcacga cgccaattcc ctgcgtgcct tcaaccaact
    25741 tgaccaccag cggcgcacca ccgaccatgt cgattaaatc gctggtatca tccggcgaat
    25801 gcgcaatgcc cgtgacaggc aggtcgatgc cctgacgcgc cagcagttgc atggaacgca
    25861 atttgtcacg cgcccgggca atggcgaccg actcattgag cggatagctc cccagcatct
    25921 cgaactggcg cagtgccgcc gtcccataaa aggtaatggc ggtgccaata cgcgggatca
    25981 ctgcgtcaaa atggggtaac ttgcggcctt tgtagtgaat agaagacgcc gcaggattta
    26041 tgttcatgta gcaagaaagc ggatcaagaa tttcaaccag gtgaccgcgc tgtatagcgg
    26101 cttcacgcag ccgcttacac gaatagagcg ttccatcccg ggacaatatg gcaattttca
    26161 ccctgcacct ctctgtcaga cctggtcaaa agcccgcgta tcatacatcg acgtggcggt
    26221 tttagcgcgt cgcccaaccc tgtttgtgca aataatccag aataaatggg cggctttctt
    26281 taatgattgt tcggcggata tgatcgctcc aggtatcccg gcgattattg ctgccacggg
    26341 tgaggtaata ttccgccagt tgctcgtcat actgcgccag tgcgtcttta tccagcggtt
    26401 gatagctgtt ttcatgcacc aaaatggagg ccggtaaacg cggcttgaga tccggattat
    26461 ccgcaggcca gccaaggcac agcccaaaca gcggcagaac atgctgcggt aatttcagca
    26521 gtttcgtcac cgcttcaata ttattgcgca ggccgccgat atataccccg cc
//
LOCUS       DAETQN010000052        25773 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.052, whole genome shotgun sequence.
ACCESSION   DAETQN010000052 DAETQN010000000
VERSION     DAETQN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25773
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.052"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..827)
                     /gene="dgt"
                     /locus_tag="K0W68_002159"
     CDS             complement(<1..827)
                     /gene="dgt"
                     /locus_tag="K0W68_002159"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBI3073712.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
     gene            911..1609
                     /gene="mtnN"
                     /locus_tag="K0W68_002160"
     CDS             911..1609
                     /gene="mtnN"
                     /locus_tag="K0W68_002160"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBI3073713.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            1602..2402
                     /gene="btuF"
                     /locus_tag="K0W68_002161"
     CDS             1602..2402
                     /gene="btuF"
                     /locus_tag="K0W68_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBI3073714.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            2440..3063
                     /locus_tag="K0W68_002162"
     CDS             2440..3063
                     /locus_tag="K0W68_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBI3073715.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="K0W68_002163"
     CDS             complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="K0W68_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBI3073716.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3447..3512)
                     /locus_tag="K0W68_002164"
     CDS             complement(3447..3512)
                     /locus_tag="K0W68_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073717.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="K0W68_002165"
     CDS             complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="K0W68_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBI3073718.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            5182..6462
                     /gene="hemL"
                     /locus_tag="K0W68_002166"
     CDS             5182..6462
                     /gene="hemL"
                     /locus_tag="K0W68_002166"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBI3073719.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="K0W68_002167"
     CDS             complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="K0W68_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBI3073720.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="K0W68_002168"
     CDS             complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="K0W68_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBI3073721.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="K0W68_002169"
     CDS             complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="K0W68_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBI3073722.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="K0W68_002170"
     CDS             complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="K0W68_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBI3073723.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="K0W68_002171"
     CDS             complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="K0W68_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBI3073724.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="K0W68_002172"
     CDS             complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="K0W68_002172"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBI3073725.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            17979..18509
                     /gene="thpR"
                     /locus_tag="K0W68_002173"
     CDS             17979..18509
                     /gene="thpR"
                     /locus_tag="K0W68_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBI3073726.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            18524..19228
                     /gene="sfsA"
                     /locus_tag="K0W68_002174"
     CDS             18524..19228
                     /gene="sfsA"
                     /locus_tag="K0W68_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBI3073727.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            19406..19861
                     /gene="dksA"
                     /locus_tag="K0W68_002175"
     CDS             19406..19861
                     /gene="dksA"
                     /locus_tag="K0W68_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBI3073728.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            19898..20824
                     /gene="gluQRS"
                     /locus_tag="K0W68_002176"
     CDS             19898..20824
                     /gene="gluQRS"
                     /locus_tag="K0W68_002176"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBI3073729.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            20863..22281
                     /gene="pcnB"
                     /locus_tag="K0W68_002177"
     CDS             20863..22281
                     /gene="pcnB"
                     /locus_tag="K0W68_002177"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBI3073730.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            22278..22757
                     /gene="folK"
                     /locus_tag="K0W68_002178"
     CDS             22278..22757
                     /gene="folK"
                     /locus_tag="K0W68_002178"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBI3073731.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            23127..23711
                     /locus_tag="K0W68_002179"
     CDS             23127..23711
                     /locus_tag="K0W68_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3073732.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            23816..24556
                     /locus_tag="K0W68_002180"
     CDS             23816..24556
                     /locus_tag="K0W68_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBI3073733.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            24591..25773
                     /gene="htrE"
                     /locus_tag="K0W68_002181"
                     /pseudo
     CDS             24591..25773
                     /gene="htrE"
                     /locus_tag="K0W68_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT         6330 a         6983 c         6416 g         6044 t
ORIGIN      
        1 tctgccacac aataggagat gtcgtcggca gcttccataa tccacgttaa tggaaaacga
       61 ctgtaaagcg ccaaattaag ttctttacgc aaccgggcaa tataggcttc ttcagaaaga
      121 taataacccg gctttttcat taaatagtga tgtgtctcag gcgtttcgcc acgccaccac
      181 gccggacggg tatattttaa aataccgcca acctgtgccc aagtgagatt catccgcatc
      241 aatgtatgca ccagacgaat gccttgtgca ttcccctcaa aatgacataa gtcctgacga
      301 atcttgcgcc gcagctcgtt aagctgttct tccccgtccc gtaaacgtag tgccgccacg
      361 ctgcagcgat cgtcagtcag aggctggctt tcggcatctt ccgggtgcaa acgttggcga
      421 aaccagtcat ttatcgctgc ttcgccaaaa tgaccaaacg gcggattgcc gatatcgtgc
      481 atcaggcatg acatctcaac aatgctttca aaggggccgg tcagttcatc caggccgtat
      541 gcttccagca atttaagctc tttcagacgg cttaaaattt ctttggcgat gtagcgtccc
      601 acctgctgga cttccatcga gtgggtaaga cgcgtgcgca cggcggcatt gcgctccagt
      661 ggaaaaacct gggtcttttg ttgcagacga cgaattgccg gagagttgat gatacgcccg
      721 cgatcgctct cgaagatccg caggatctca tgttcggttt taacgccctg cggtgaacgg
      781 taacgacgat gccagtttat tttttttcgg aaatcaatct gtgccatgtc ctctcccgcg
      841 tgagaaatac gcttccccgt aagcgcatgg taaactatgc tttcaaatcg ggcttatcgc
      901 gagtaaatct atgaaaatcg gcatcattgg tgcaatggaa gaagaagtta cgctgctgcg
      961 tgacaaaatc gaaaaccgtc aaactatcag tctcggcggt tgcgaaatct ataccggcca
     1021 actgaatgga accgaggttg cgcttctgaa atcgggcatc ggtaaagtcg ctgcggcgct
     1081 gggtgccact ctgctgttgg aacactgcaa gccagatgtg attattaaca ccggttctgc
     1141 cggtggcctg gcaccaacgc tgaaagtggg cgatatcgtt gtctcggacg aagcgcgtta
     1201 tcacgacgcg gatgtcacgg catttggtta tgaatacggt cagttaccag gctgtccggc
     1261 aggctttaaa gctgacgata aactgatcgc tgccgctgag gcctgcattg ccgaactgaa
     1321 tcttaacgct gtacgtggcc tgattgttag cggcgacgct ttcatcaacg gttctgttgg
     1381 tctggcgaaa atccgccaca acttcccaca ggccattgct gtagagatgg aagcgacggc
     1441 aatcgcccat gtctgccaca atttcaacgt cccgtttgtt gtcgtacgcg ccatctccga
     1501 cgtggccgat caacagtctc atcttagctt cgatgagttc ctggctgttg ccgctaaaca
     1561 gtccagcctg atggttgagt cactggtgca gaaactggca catggctaag tcactgttca
     1621 gggcgctggt cgccctgtct tttcttgcgc cactgtggct caacgccgcg ccgcgcgtca
     1681 tcacgctttc tcccgccaac actgaacttg cctttgccgc cgggatcacg ccggttgggg
     1741 tcagcagcta ttccgactat cctccacaag cgcaaaagat tgagcaggtt tccacctggc
     1801 aggggatgaa tctggaacgc attgtcgcgc tgaaacccga tctggtgatt gcctggcgtg
     1861 gaggtaatgc cgagcggcag gttgaccagc tggcttcgct gggaataaaa gtgatgtggg
     1921 tcgatgcgac aagcattgaa caaattgcca atgcgttacg tcaattggcc ccctggagtc
     1981 cgcaaccgga taaggccgaa caagccgcgc aatccctgct ggatcagtac gcgcaattga
     2041 aagcgcaata tgctgataaa cctaaaaaac gtgtttttct gcaattcggc attaatccgc
     2101 catttaccag tggaaaagag tcgattcaaa accaggtact cgaagtttgt ggcggagaaa
     2161 acatctttaa agacagccgg gttccctggc cgcaagttag ccgcgaacag gtgttagcac
     2221 gctcgccaca ggcgattgtc attacaggcg gaccggacca aattcctaaa atcaaacaat
     2281 actggggtga acagctcaaa attcccgtta ttcctctcac gagtgactgg tttgaacgtg
     2341 caagcccacg tattatcctc gctgcacaac agctctgtaa tgcgctttca caggtagatt
     2401 agcgcctgac ctttttaggt gctcagtggg gattgaacaa tgctcgtcta ttggctggat
     2461 atagtcggca cagcggtatt tgccatctcc ggcgttttgt tagccggaaa attgcgtatg
     2521 gacccttttg gtgttctggt actgggcgtg gttaccgctg taggcggcgg gacaattcgc
     2581 gacatggcgc tggatcacgg cccggtattt tgggtgaaag atcccaccga tctggtcgtt
     2641 gcaatggtaa ccagcatgct gaccatcgtg ctggtgcgcc agccaagacg cttaccaaaa
     2701 tggatgttgc cggtgctgga cgccgttggt ctggcggtgt ttgtcggcat tggcgtgaat
     2761 aaagccttta atgcggaagt cggtccgtta atcgcggttt gtatggtcgt cattactggc
     2821 gttggcggcg ggatcattcg tgatgtactg gcccgcgaga tccccatgat tttacgtaca
     2881 gaaatctacg caactgcctg tattatcggc ggtattgtcc acgctacggc ttattacaca
     2941 ttttccgtac cactggaaac agccagtatg atgggcatgg tcgtgacgct attgattcgg
     3001 ctggcggcta ttcgttggca tcttaagcta ccgacgtttg cgctggatga gaatgggcgt
     3061 tgaagagaga agatgcgccg gatagtttat ccggcacatg aacaacagat tagatgctaa
     3121 aggaagaacc gcaaccgcag gtgcttttcg cgttcgggtt ggtcacgatg aaacgagaac
     3181 cttccagtcc ttcggtatag tcaacggaac cgccgaccag atattgcagg ctcatcggat
     3241 caaccaccaa gccaacgccc tgtttttcga tggtcatatc gccttcgttc acctgatcat
     3301 caaaggtgaa accatactgg aagccgctgc aaccgccacc ggtgatatac acgcgtaatt
     3361 tcagattcgg gttatcttcg tcagcgatca ggcttttaac tttgttggct gctgcgtcgg
     3421 taaactccag cggcagtgct acgtcatcac tcatattttg ctccaaacga catcggcaat
     3481 tgggcaaatt ctaacccaat aataatcgcc attatctaat accctggtat ttcgttcaag
     3541 tattctcgct ggctgatgcg gccttgcttc gcgccagttg ctcagcttcc tgttttgcca
     3601 gcgtgcgcgc aagaatcgcc gagtatagcg gtttcccgcc ggtaaattgc gctaatagtg
     3661 ttgcgccaag accggtaata atcattggca aaatgagctg gtagttatcg gtcatctcca
     3721 gaaccagaat gatccccgtt aacggcgcgc gaatagatgc cgccagtaat gcccccattc
     3781 cggcaatagc aaacgtcccc gcctcaaggt gatattgcgg aaacagctca acggcaacca
     3841 ttccgaaagc ggttcccagc acagtaccca gcgccagcat cggggcaaaa ataccgcccg
     3901 gcgcgccgga agagaagcag agtaaggtgg taatgacccg agcgacgaag ataaacacca
     3961 gcattcccat gctgaaattc cccgcggtag cgataggaat caggttaaaa ccgccgcccg
     4021 acgttgctgg tgccacaaac cccagcaatc cacacagacc gccaatcgca ccgcccatca
     4081 gtacccattt ggtaatattg ccgccgtgca cacggtgcag caaatcctgc atccccagca
     4141 cccatttatt aaaaataggg ccgaaaatgc caaaaataat accgaggatc agataaagcc
     4201 acagcgtatt aagcggcgca tcagaaagtt taccgacgtc aatcaacgca acttcatgat
     4261 taaaaatccg gtacataatg gtcgacataa tgacgccaat aaataccgct tttatcgaaa
     4321 ttaacgtata gcgaaactgc ggacgcatct cttcgataat aaacaaaata cccgccagcg
     4381 gcgcgttaaa ggccgcagcc agccccgcag cagcaccggt tgccagcagc gtatggcgag
     4441 cttcgtcacc tttcaggcgg aaaatatcaa gcaccatacg gccaatgtta ccgccgatct
     4501 gcacggttgg cccttcgcgc cccaacacca tgcctccgcc gagtgtcccc agcccgccaa
     4561 agaacttcac cggcaataca cgccaccagc gaacgggacg ttgatcttcc agcgcccctt
     4621 caatttccgg gatccccgaa ccacctgctt ccggcgcgta tttgcgcacc aaaaagtagc
     4681 caaacatcgc cagcaccgcc gaacagagaa aagcgacggt taacagaagc ggataattat
     4741 cagcagtatg taccagcgcc cccatacgtt ggttctgcaa ccaggcgaca cctttgtcaa
     4801 aagcaaccgc tgccagccca acaagcgtgc cgacgactgc cgccataaac aaaatggcta
     4861 acggggtttt atcgcgctca agaagttggc gaatcagttg tctgcgtcgc aggcgcgcgg
     4921 cctgcggtgt ttctaaagag ggagtatcag ttttcatcca atgatcactt attggtcata
     4981 caaataagat gacggcattt tactcgcacg ttccatgaaa atcctttgca aagcgtaatg
     5041 tttcaaatgc acaaaacttt tatactctaa gaaatcagga ttgcaccagt acaagcagcc
     5101 tgatgtttga cgagtattta acttgttatg aataacatag aatagcagcc attcactttt
     5161 tctacgaatc aggaaccctc catgagtaag tctgaaaatc tttacagcgc agcgcgcgag
     5221 ctgatccctg gcggtgtgaa ctcccctgtt cgcgccttta ctggcgtggg cggcactcca
     5281 ctgtttatcg aaaaagcgga cggcgcttat ctgtacgatg ttgatggcaa agcctatatc
     5341 gattatgtcg gttcctgggg gccgatggtg ctgggccata accatccggc aatccgcaat
     5401 gccgtgattg aagccgccga gcgtggttta agctttggtg caccaaccga aatggaagtg
     5461 aaaatggcgc aactggtgac cgaattggtc ccgaccatgg atatggtgcg catggtgaac
     5521 tccggcaccg aggcgacgat gagcgccatc cgtctggccc gtggttttac cggtcgcgac
     5581 aaaatcatta aatttgaagg gtgttaccac ggtcacgctg actgcctgct ggtgaaagcc
     5641 ggttctggcg cactcacgtt aggccagcca aactcgccgg gcgttccggc agatttcgcc
     5701 aaacatacct taacctgtac ttataacgat ctggcttctg tacgcgccgc gtttgagcaa
     5761 tacccgcaag agattgcctg tattatcatc gagccggtgg caggcaatat gaactgtgtt
     5821 ccaccgctgc cagacttcct gccaggtctg cgtgcgctgt gcgacgaatt tggcgcattg
     5881 ctgatcatcg atgaagtaat gaccggcttc cgcgtggcac tggctggcgc acaggattat
     5941 tacggtgtgg aaccggatct cacctgcctg ggcaaaatca tcggcggtgg aatgccggta
     6001 ggcgcattcg gtggtcgtcg tgatgtaatg gatgcgctgg ccccgacggg tccggtctat
     6061 caggcgggta cgctttccgg taacccaatt gcgatggcag ccggtttcgc ctgtctgaat
     6121 gaagtcgcgc aaccgggcgt tcacgaaacg ttggatgagc tgacaacacg tctggcagaa
     6181 ggtctgctgg aagcggcaga agaagccgga attccgctgg tcgttaacca cgttggcggc
     6241 atgttcggta ttttctttac cgacgccgag tccgtgacgt gctatcagga tgtgatggcc
     6301 tgtgacgtgg aacgctttaa gcgtttcttc catatgatgc tggacgaagg tgtttacctg
     6361 gcaccgtcag cgtttgaagc gggctttatg tccgtggcgc acagcatgga agatatcaat
     6421 aacaccatcg atgctgcacg tcgggtgttt gcgaagttgt gatccagcat ggtttgtcgg
     6481 atgcggcgtg aacgccttat ccgacctacg attcggggcc gtttgtaggc ccgataagac
     6541 gcgccagcgt cgcatcgggc atccgttgtc gcgtaaacgc tttatccgtc caggcgtaca
     6601 gggccgttat atggaaaaat taacggctct gctttctcag caaatagata aaatatggcg
     6661 cgccgataaa ggtcgacagc agccccgccg ggatctggaa tggaaacagc accatccgcc
     6721 cacaccagtc agcgaatacc agcagtaaac cacccaccag cgccgaaatt acgatgtgtg
     6781 gcatcgtccg tcgaaagccc atcatccgcg caatatgcgg tgccattaaa ccaacaaaac
     6841 tcaacggtcc aatagtcatc gtcgcggtcg ccgtcaggca agccgctaac agcagcagcg
     6901 caattcgcgt cggcgtcagc gccattccta cggctcgtgc ggtatcacca cccagcggta
     6961 aaatggtcag ccagcggcgg tacagcgggg taatcgccag caaaatcacc atcacaattc
     7021 cggtacgcca gacctgcgca tcggtcgcgt tgtaggtcga accggaaatc caggtcagca
     7081 cttgcgccat tcgcgggtca ccgctcgcct gcaacatcat caaaagcatg gtgaatgcgg
     7141 tacttaacgc catccccgcc agtaacatac ggtgtgggga aaatccaccg cggccggcgg
     7201 cgatcataat gatcaacagc gtcaccgccg ccccgatact gcccgcgggt aacagccagc
     7261 caaaggcatt acccggcacc agaaacagca ttaacaccac gccaaacgcc gcgccagagc
     7321 taatccccag cacttccggg cttgccatcg ggtttccggt cagccgctga ataatacagc
     7381 ccgccaccgc cagcatgacg cccgcaaaca gcgccgccat aattcgcggc cagcgccagg
     7441 gcatcaaatc ctcgagcaac gccccgctcg cccacgtcca gccgtgcgca tcacgaccaa
     7501 acgacagcgc caccaccaca gccatcaaca gcagcacgcc gcccgcgagg gcaaacgcca
     7561 gcacatgttg gcgttcagcc gcgacacgat cgttgacctt catatccggc gcgctaatgc
     7621 tgcgtaaacg cggcaacagc cacagtagca gcggcgcacc gatcaacgca gtgaccgaac
     7681 cggtggacac ttccatccac acgcgagtca gccagaggat gatttgatcg gaaagccaga
     7741 ggatcagcgc cccaatcagc gacgccagca tcaaccgtgg caacaaacgc cgcgccccca
     7801 gcattttcgc cagcagcggc gcgaacaaac cgataaagcc gataatcccc accgcattca
     7861 ccagcagcgc actgatgact atcgccagcg acagcgccgc cagacgcgcg agcgacaagg
     7921 ccagcccgag attgcgcgcc acgccatcat caagccccat cagggttaac gggcgaagta
     7981 gcaataacgt caacatcacg ccgcccagca actgcggcca taaacgctca acgccgcccc
     8041 agtcggtttg cgtcagcgtt ccggtgctcc acaggaacat gctttgcagc tggtcgtgat
     8101 ggaagataac cagtagctga ttaattgccc cgcaataaag gctcaccacc agccccgcga
     8161 gaataagcgt aaccggcgaa agccgtttcc cccacgcgac gccaaagacg attaagccaa
     8221 cgacacaagc gcccaccagt gcggcaaact ggctcgccat agcacctggg atcgcccaaa
     8281 gtgtggtgac ggtaattccc agttgcgcgc ctgtagcaac gccaagcgtc gtcggctccg
     8341 ccagcgggtt acgcagcact tgctgaaaca gcacgcccac cagccccagg cccgcgccca
     8401 ccagcagcga aatcgccaga cgcggcaaca agctgtagtg aaaaatcatc tgctcgatga
     8461 catcgatgtc tggcgaccag gcagcctgcg cccactggct acgcggcagc gcctgcgaga
     8521 agttcatcca ggtgagcgcc gtagcgacaa tcactaacag cgccagcaat aacgccggaa
     8581 aaagcgcaat tcgtttactc acgctttacc tccgatggcg ttatccagaa cgcgcacaaa
     8641 gtgcattgcc gagagtgtcg caccataaaa ccagactgca ggtacgcgct gaaagcgtcc
     8701 ggcgcggaca aacggcatgg cctgccacag cggggttgcc attagcgcat ccatgtcttt
     8761 gctgttgtcg tgatcaaaac agagcacatc aacgtcttta tacgctgcca gacgatcgat
     8821 actgacggcg gtactgcccc agaagttggt ttccccttgc caggcatttg ggatgccgta
     8881 ctcatcgaga atttcctgga acaagctgtt tggaccgaag accagcatat ggcgcggatc
     8941 gataagcgtc gtcagcagta acgggcgtgc accacgcttc acaaagcggg gtttcatgct
     9001 gcggataaag tcttcgtatt gcgctaaatg cgtttccgct gcgctttgca ggttaagtaa
     9061 atctgccatt tccgtcagcg atttacgcgc catcgccaac ggctgtttgc cgtcactgaa
     9121 gttaaatccg cgacccggcg caatacgtgc cagcatttct gatgaagggc catatcctgc
     9181 cgaccagacc ataaacgacg gtttcatttc ggtcagcagt tcaaggttag gttctgtacg
     9241 caaaccgacg tcaatcactg agtccggcaa tggtggttcg ctgacccaca ggcgatagtt
     9301 gatggtatcc gccacgccgt aaggcacgat gccgagcgcc agcagtaatt ccaccggcaa
     9361 ccactccagc gccacaatac gattggggtc aatagccgcc gcgtgggcgg tattcatctg
     9421 ccataacaac ggagaaagcg ccatcgccgt taacagtcga cggcgcgaaa taagaggtaa
     9481 gccgctcatc aataaacaaa actcacaggt gcagcacccg ccggatgcgg caaaataccc
     9541 atcgggatgc cataaatcat ttcgagggtt tcgccgcgca taatttccgc aggcgttccc
     9601 tgagcaatca tttcaccact gcgcagggcg accagataat cacagtagcg tgccgccata
     9661 ttgatatcgt gcaacacggc aatgaccgtc aggccacgtt cctgacttaa acggtgcacc
     9721 agcgccagca catcaacctg gtgggcgata tccagcgccg aagtcggttc gtcgagcaac
     9781 agacaacggc tatcctgcgc caccagcatg gcgatccacg cccgctgacg ttcgccgcca
     9841 gagagactat cgaccagccg atgcgccagc ggttttaagc caaccagcga gatagcttcc
     9901 tcgacctttt cgcgatctgc cgcaccaaag cgccccagcg cgccatgcca cgggtaacga
     9961 ccaatcgcca ccagttcacg cacggtcatc ccttctgccg gaggaagctg ctgcggcaaa
    10021 taagccactt tgcgggcaaa cgctttgctg ctccagcttt ccagcggctg ggcatcaaga
    10081 agaatctccc cttctgacgg aggctgatga cgaccaagca ttttgagcag agtggattta
    10141 ccagaaccgt tgtgaccaat cagaccggtc actttcccgg caggaaaggt taacgacagc
    10201 ggatgcaaaa gcgtgcgccc gggcacacga aaggagatat tacgcagtgc aaaagtggta
    10261 tcggaatgat tcgtgtattc ctgcataaca gccaacttgt gaaatgggca cggaaatccg
    10321 tgtcccaaaa gagaaattag aaacggaagg ttgcggttgc aacgacctga cgttctgcgc
    10381 cccagaagca gccataagtc tggaagcaac tggcaacata ctcacgatcg agcaaattgt
    10441 tgacgttcac agcaaggctg gatcccgcca taccaaagcg tgccagatca tattttacaa
    10501 cagcatccat tacagctgtg ctacccactt taaaagtgtt tgccggatca ccatagctgg
    10561 aaccaataaa acgaccacct gttcccaatg ttaaaccaga aagcggccct tcattgaagg
    10621 tataatcccc ccagagagat gccatatgct ccggtacttg ttctggagtt ttacctttga
    10681 gattggtgtc tttggtgtat tccgcatcgg tgtaggtata agaagccgtc aagttaatat
    10741 tggcatttaa cgccgctttt gcttcaagtt ccacaccacg agcgcgaatc tcacctgctg
    10801 ggacttgcgc taatgggttt gttgggtctg ctgtcaggtt atttgttttc gtcaattgat
    10861 agactgcgcc cgtaacaacg accggcatat cattcggaac atattttacg ccagcttcat
    10921 actgttcacc tttcgatggc ttataggaaa tgcgcgggtt gctccacaga tcgaaagcac
    10981 tcggttcaaa ggactggcta tagctaaagt aaggtgaaat accattatcg aataagtaat
    11041 ttacaccacc gcgccaggtg aactgatgat cattgcgctc aatataacct tcagtcggcg
    11101 tataagagtt ttcacgaaca gtggtcgctt gcttagacca atcgtagcgt ccccccagag
    11161 tgaataccca tttattccat tccgcctgat cctgaacata aatacctgtt tgtttgcttt
    11221 cattcatctg gtatggctct gcgctaccaa atgcaaagta ttcagggtga tatttgttat
    11281 aaagatcgat ggatggtgcg gatccaaagc tggcattgat atcattacgc atccgcatga
    11341 agtctacccc tgtcagcaaa gtatgctcga cgtcacctgt agcaaactta ctttcaagtt
    11401 gggtatcaac gctaaagttt tgtagacgct cattatccac cactgtcccg cggtttaggg
    11461 tatgaccatc accagcaatc cctgtgccat aaacactttt ctgtgaggtt ttcatttcac
    11521 tgaaacgcag attctgacgc acggtgaagg tgtcattaaa accatgttca aaactatatc
    11581 ccaccatttt ctggttacgg gagtatgtat tattcgatgc accttcattg aagtcagtcg
    11641 gtagacgctt accattaggc aatggctcaa cggtcccctc tttcggcaac caaccgtaat
    11701 aacccgtttc tggttcattc tggaaatagg acaggaaagt aaaattggtt ttgtcgtctg
    11761 gacgccatga gaatgatgga gcaatggtat aacgctgaga ttcagagttt ttctgctgtt
    11821 cgtttgttga gcgagcgagg cccgtcaaac ggtaagagaa ctcaccgtta tcatccagtg
    11881 catcactgaa atcgaatccg gtctgaaaca gattatccgt ccccatttta aactgaattt
    11941 ctttcagggg ttcagtggtc ggacgcttac tcaccataga aataatccca ccaggattac
    12001 tttttccata gagaacagac gtcggcccac gcatcagttc aaccctctcg agcatgtaag
    12061 gatcaatcac agcatcgtta taaaagttgc cctgaagttt tagtccatca aggtaattat
    12121 tttgattcag accaacagat gagaaaccac gaatgataac aaaatcatat gtattagaag
    12181 caccacgact gctaaccgta acgccaggtg tataacctaa tgcttctttt acagactgaa
    12241 attgacgcat ctgcatctct tcatttgtca caaccgaaat agactgcggc gttttttcaa
    12301 ttggtgtatc cgttttagtc gcagtagcag agtgcttcgc cgcaatagtt gcagcaggcc
    12361 cccatgcgct ttcttgcggc gcaggtgcag cggtaacggt gatagtgtct tctttcggtt
    12421 caaccgctgc ctgtgcataa acagacatgc cgctaaccgc tgtggctact acaactgcga
    12481 ttttacgcag tgagtgtttt ggctgagcag ttttggaacg cgccattggt atatctctga
    12541 tgtaaagtga atgataacgt aaacgagaat gattattata aagataagat aatattcgaa
    12601 acgctggcgg gttcgcaagc ggtacgcaat ctgagcgttc ctgaagggtt aataaacaac
    12661 cagatgaaaa gaaagggtta atactttagg tgggatgtta ttttaccgga tggcaattcg
    12721 ccatccggta tttcacgctt agatgttaat tactaccaaa catatccttg atccaaccgg
    12781 ctacaccgtc gctgtctttc tgctcttgct gagcaggttg ctgttgcggc tgttgctgcg
    12841 gctgagaaga ctgatcaaac ggattgcctg acggctgctg ctgcatctcg ctctgctggc
    12901 acagcgattg cggatcgctg gtccagaccg gcaagacacg catgccaccg ctgcaaacaa
    12961 agttgccgtc gtagtccacg cccatatctg caatatcttc tggcggaaca agattcagcg
    13021 gcgttggcgt ctggttagcc agataacgct gataaataga cattgccccg ctggcaccat
    13081 acagtttggt cggctggttg ttatcacggc ctacccaggt gatggtcacc gtgctgccgt
    13141 caatgcccgc aaaccaggta tctacgttat tgttggtagt ccctgttttc cctgccagat
    13201 gcaggttcgg gtatttcgcc ccaagttgac gacccgtacc acgttgtacc acctgctgca
    13261 tggtccatag tgtcagatac gccgcctgcg ccggaacagc gcgttctgcc tgcgggaagc
    13321 tctgatacag caccttgcca tcttccgcga ttaccgaacg cagtgcagaa agcggtgcac
    13381 ggttaccgcc gctggcgatg gtctggaatg cctgcgccac ttcgattggc gttaagttca
    13441 acgcccccag cagcatcgcc ggaaccggat gcagctgatc tttcggcacg cccagtttga
    13501 tccaggtctc cgtaactgca ggcagcccca gcgccatccc cagatttacc gtcggcacgt
    13561 tcatcgaacg ggtcaacgca tccaccagca tcactctgcc gctttcgcta taacgacggt
    13621 catcgttctg cggtgaccag acctggccat tcggctgacg cagcgcgatt ggcgcatcgg
    13681 caatccacgt gttcaggcga tagattttcg gctggcttaa agccgtcaga taagtcgctg
    13741 gttttgcaag ggaaccaatc gaacgacgcg cctgcatcgc acggttgtag cccgcaaact
    13801 gcggctcaga acctcccacc atcgcacgaa cttcaccact aaagcggtcg acgaccacaa
    13861 ttgcagtttc aagatcgctc aacttacgct gtttcttcag tgccggaatg ccttccacgg
    13921 cggctttttc tgccgcgtcc tgggccaccg agtcaaaggt agtgaagatc ttcacgccgg
    13981 agagatcttt taccttatcg cctaatttcg cctgcagctc ctgacgcacc agttgcataa
    14041 aggctggctg tggagagatt accccaccgc gcggctgtac gcccagagga cgcgcgctga
    14101 gcatttcata cagttcacca tcgataatct gctgttgttg tagcaaacgc agcaccagat
    14161 tacgtcgctc cagcgccagt ttcgggttac gccacgggtt gtagatggac gcccctttca
    14221 ccataccgac taacagcgcc tgctggtcga ggctgagctc ttctaccggg cgaccaaagt
    14281 aatacaagct cgcaagcggg aagccgcgga tttcgttgtc gccgctctga ccgagataca
    14341 cctcgttcat atacagctca agaatacggt ctttgctgta acgcgcgtcc atgatcagcg
    14401 ccatgtaagc ttcgttcgct ttacgccagt aagaacgctc gctggagagg aacaggtttt
    14461 tcaccagctg ttgcgtcagc gtactcgctc cctgcaccgt gcgtccggcg gtcaggtttg
    14521 ccagcaccgc acgtccgatg gagtagagac tgatcccatc atgctcgtag aaatgacggt
    14581 cttctgtcgc cagcaaagta tccaccagca agtccgggaa accactgcgc ggcacaaaca
    14641 gacgctgctc accgtttggc gaagagatca tggtgatcag gcgcggatca agacggaaga
    14701 aaccgaactg acggttgttc tccatattga cgatcgtcgc cagatgatcg ccatcaaagg
    14761 tcagacgcgc acgcacctgc ccttctttgc tgtccgggaa atcaaacgga cggcggatca
    14821 tctcaatgct gttggcctgc acggtaaatt cgccaggacg ggtcattttc gacacctgac
    14881 gatactgtgt tgcctccagc agcttcacca tttcgttctt gctgatggtc atatctggtt
    14941 caaggttgac cattcggcca taaactgccg cgggcagttg ccagaccttg ccatcaatac
    15001 ggctacgaat tttttgatcg agataaacgc cgtaaatggc gatcagcacg gcaaaaacga
    15061 tagccagttt taacagcagc catagccagc cgcgtttgcc acgaggctta cgccctttgc
    15121 ctttgccctt acctttgcgc ggcatcggtt cttcatcctc atagtcatca taatcgtcgt
    15181 aatcatcgtc atcttcgtaa cgacgacggc ttaccttttg tttgaccgga cgcgtcggtt
    15241 tccctttgcg tccaattggc tcgcggtcat tcccggccat gctttttctc cgcaatattc
    15301 aggcgcaaaa gcccgatttt ctgttcttct gttacaagac agaagaagat atctctcaaa
    15361 atttgtgctc gatcggtccc ctctcccttg agggagaggg ttagggtgag ggtgaacgtg
    15421 cgcgctgatg ccctcacccc ggccctctcc cacagggaga gggagaaaac caaccttacg
    15481 aatacttttt cgtccgtcgc gtcggtgccg tgtttgccgg gtcgtccggc cagacgtgtt
    15541 tgggataacg ccctttcatc tctttttgca cctcacggta cgctcctttc cagaaggcgc
    15601 tcaaatctcg tgtgatttgt agtggccttt gggcgggtga aagcaactcc agcaccagcg
    15661 gcacgccccc ctgggcgata gtcggattag tggcctcacc aaacatctcc tgcattctca
    15721 ccgccagtgc gggcgggtta tcttcatgat aacgaatggc gatccggctt cccgtcggca
    15781 cagtgtaatg cgcaggcaat tcactatcca gacgttgctg cattccccaa tcaagtaatc
    15841 cgcgtagtgc ctgataaatg tccagtgatt tcaggccgcg tagtgaatgt acgcctgtca
    15901 tatgtggcag cagccacgtt tccagcgctg ccaataaact ttcatcatca accgctggcc
    15961 agtcatattc cggcaaccac tttgcggcgc ataacaaacg caagcgtagc tgttccgctt
    16021 ccgccgtcca gttgagcacg cttaaacctt tatcacggat gccattaagc atcgcctgat
    16081 gcaactcgtc ttctgacggt ttcgccagcg gctgcacttt caccgtcaac tgaccgattt
    16141 gtagccgacg ccaggctttc agcgtacctt gcgcgtcatc ccattccacg gtgtcagatt
    16201 gctgcaccag ctgcggacag cgttgtacta attcatcaat atcgaccggt agcgccagta
    16261 aaatccgcgc atccggcgag gcgctgccct gcaataataa cggtgcgatc aaccattcgt
    16321 ggcggcttag cgcgtcgtcg gcatcgagca tcgctcccat gccgtttgcc agttgatagc
    16381 gtccatcttg cccacggcga tgagcaatgc gatcggcaaa cgccccggca agtagcggcg
    16441 cgataagcga actgtctgcc tcaccgccgc gtacgtttaa gcgttttaac agttgctgac
    16501 tacgttgctg ccaggctggt tgattgcgcg aaaacgccac gcccaggtca ctattgccca
    16561 tcagtggcgg ctcttcgaga atggcggcaa tttttgccgc ggtagcagct tcgtcgtcgt
    16621 tcttcgcgct aaccagcatt gccgctaaac gcggatcgtt acccagcgct gccatttttt
    16681 gcccttgcgc actaagccgt tcaccgtcca gcgcccccag catttgtaac agacgtttcg
    16741 cggccattag attcactact ggcggttgat ccagccagct catctgcgcc ggatcgctgc
    16801 atccccattg cagtaattcc atcagcaaac cggaaagatc gctttgtaag atctccggtt
    16861 cactttgcgc ggcagcgcgt tctgcttgtt ctttggcgat taaatgcagg cagatacccg
    16921 gctccagacg cccggcgcgc ccggcacgct gcgtcatgga tgcctggcta acgcgttgag
    16981 taatcagtcg cgtaagcccc gtgcgcggct caaaacgcgc cacacgctcc tgggcacaat
    17041 ccaccaccag acgaatacct tcaatggtta aactggtttc agcaatattg gtcgccagca
    17101 ccactttgcg catcccttgc ggtgccggga ggatcgcttt tcgctgatcg ttcagcgaca
    17161 acgcgccata cagcgggcag agcaacacat cactgccgat gcgcgaagcc agttgttcct
    17221 gcacacgctg aatttctccg acgccaggta aaaataacag taatgatccg ctttcctgac
    17281 gcagcatttc ggcggtggct accgcaacgg cttcgtcaaa acgctgatgc gcgggcagcg
    17341 gtaaataatg gcgttcaacc ggaaacgagc gcccttctga gatgacgacg ggcgcttctg
    17401 gcagcatttg ctgcaagcgg tcgttatcca gcgtagccga cataatcagc agtttaaggt
    17461 catcacgcag accttgttgc acatcgagta acagcgccaa cgccaaatcc gcctgcaagc
    17521 tgcgctcatg aaattcatca aggatcacca atccaacacc gctcaactcc gggtcacgct
    17581 ggatcatccg cgtcagcacg ccttcggtaa ctacttccag acgggtattt ggcccgacgc
    17641 agttttgcgc acgcatccgg tagccaacgg tatcgcctgg cttttcgtta agcagctccg
    17701 ccagccgttg cgcgacgtta cgtgccgcca gacgacgcgg ctccagcagg ataattttcc
    17761 cgttaatgcc gggatgcgcc agcagttgca gcggcagcca ggttgatttc ccggccccgg
    17821 tcggcgcact taataatacc tgcggcgcac aatcgagagc ggtaagtaat tcaggtaaga
    17881 cggcagcaac gggcaacgac gacacaaaac gctccagagg gttaacattc ttcgcgccac
    17941 attgtagcat cgcggtaatt cataaccgag tgcctcacat gtctgaaccg caacgtctgt
    18001 tctttgctat cgacttacct gcagaaatcc gcgaacagat tatccactgg cgcgccacac
    18061 acttcccacc tgaggcggga cgtccggtcg ccgccgataa tttgcatctg actctggcat
    18121 ttttaggcga agtgagcgcc gagaaagaga aggcgctttc tcttttagcc ggacggattc
    18181 gtcaacctgg tttcacactc acgcttgatg acgccggaca atggctgcgt tcgcgtgtgg
    18241 tgtggttagg gatgcgtcag ccgccacgcg gcttaatcca gctggcgaat atgctccgtt
    18301 cacaggctgc ccgcagcggt tgttttcaaa gcaatcgtcc gtttcatcca catattacct
    18361 tattgcgcga cgccagcgag gcggtgacaa tcccgccgcc aggttttaac tggtcttatg
    18421 cggtgacgga gttcaccctt tacgcctcct cgtttgcccg tggacgcaca cgctacacgc
    18481 cgctaaaacg ctgggcgcta acgcaataac aaggattgtc gcaatggaat tctctccccc
    18541 tctacagcgc gcgacgctaa ttcagcgtta caaacgtttt ttagccgatg tgatcacacc
    18601 cgatggtcgc gaattaacgc tacactgccc gaatacgggt gcgatgaccg gttgtgcaac
    18661 gcgtggcgat accgtctggt attcgacttc agacaacacc aaacggaaat acccacacac
    18721 ctgggaacta actcaaagcc agagcggcgc atttatttgc gtcaatacgc tttgggctaa
    18781 caggttgacg aaagaggcta tccttaatga atcaatttca gaactgtcag gctatagctc
    18841 gctgaaaagc gaagtaaaat acggcgcaga acgcagccgt attgacttta tgttgcaggc
    18901 ggattcgcgt ccagactgct atattgaagt gaaatcggtt acgttagcgg agaacgaaca
    18961 gggatatttt cccgatgcgg tcactgaacg aggtcagaaa caccttcggg agttgatgaa
    19021 cgtagcggct gaaggccagc gtgcggttat ctttttcgcc gtgctgcatt cagccattac
    19081 acggttttca cccgcgcgcc acatcgatga gaaatacgcg caactattgt cagaagctca
    19141 acagaggggg gtagaaattc tggcttacaa agcggaaatt tctgctgaag gcatggctct
    19201 taaaaaatca ctgtcggtta cattgtagta aagtaagtaa ctggttaatt tacattctgg
    19261 tcgcgtgcgc aaatacgctt ttcctcacac agttgtcaag tgttacgttt agataattgc
    19321 tatccggaaa agcatctgct atttatagcg gcctcatttt tcccccgaac atggggatcg
    19381 atagtgcgtg ttaaggagaa gcaacatgca agaagggcaa aaccgtaaaa catcgtccct
    19441 gagtattctc gccatcgctg gggtggaacc atatcaggag aagccgggcg aagagtatat
    19501 gaatgaagcc cagctggcgc acttccgtcg tattctggaa gcatggcgta atcaactcag
    19561 ggatgaagtc gatcgcaccg ttacacatat gcaggatgaa gcagccaact tcccggaccc
    19621 ggtagaccgt gcagcccagg aagaagagtt cagcctcgaa ctgcgtaacc gcgatcgcga
    19681 gcgtaagctg atcaaaaaga tcgagaagac gctgaaaaaa gtggaagacg aagatttcgg
    19741 ctactgcgaa tcctgcggtg ttgaaattgg tattcgccgt ctggaagcgc gcccgacagc
    19801 cgatctgtgc atcgactgca aaacgctggc tgaaattcgc gaaaaacaga tggctggcta
    19861 attacagccg ttccatcacg tttaccacag gcgggaaatg ctcccgcctt attttttgtt
    19921 caaagagatg acagacacac agtatattgg ccgcttcgcc ccctctcctt ccggcgagct
    19981 tcattttggc tctctgatcg ccgcgctcgg cagctatttg caggctcgcg cccggcaagg
    20041 tcgctggctg gtacgcatag aagatatcga cccgcctcgt gaagttcccg gtgccgcaga
    20101 aactatcctg cgccagctgg aacattacgg tctgcactgg gacggcgatg ttctctggca
    20161 atcgcaacgt cacgacgcct atcgtgaagc actcgcctgg ttacatgaac aaggactaag
    20221 ttattactgc acctgtacgc gtgcgcgtat tcaaagcatt ggcggtattt acgacggtca
    20281 ttgccgggtg ttgcatcatg gaccagacaa tgccgcagtg cgtatccgcc agcagcatcc
    20341 ggtcacgcaa tttactgacc agctgcgcgg cattattcac gccgacgaaa aactggcacg
    20401 ggaagatttt atcattcatc gccgtgatgg gttgttcgcc tacaacctgg ctgttgtggt
    20461 tgatgatcat ttccagggcg ttacagaaat agtgcgtggg gctgatctga ttgaaccaac
    20521 agtaaggcaa atctcgctgt accagctttt tggctggaaa gtgccagatt acattcatct
    20581 gccgctggcg cttaatccac aaggcgctaa actttccaag cagaatcatg cgcctgcgtt
    20641 gccgaaaggc gatccacgtc cggtactaat cgcggcactt caatttctgg gtcagcaggc
    20701 agaagcacac tggcaggatt tcagcgtcga acaaatcctt cagtcagcag tcaaaaactg
    20761 gacgctgacc gccgtgcccg agtcggcaat tgtaaattca acattctcaa atgcgtcatg
    20821 ctgagctatg attagccgct atttttttgt cctgaatgat gtttgacact accgaggtgt
    20881 actattttta cccgagtcgc taatttttgc cgcaaggtgc taagccgcga ggaaagcgag
    20941 gctgaacagg cagtcgcccg tccacaggtg acggtgatcc cgcgtgagca gcatgctatt
    21001 tcccgcaaag atatcagtga aaatgccctg aaggtaatgt acaggctcaa caaagcggga
    21061 tacgaagcct ggctggttgg cggcggcgtg cgcgacctgt tacttggcaa aaagccgaaa
    21121 gattttgacg taaccactaa cgccacgcct gagcaggtgc gcaaactgtt ccgtaactgc
    21181 cgcctggtgg gtcgccgttt ccgtctggct catgtaatgt ttggcccgga gattatcgaa
    21241 gttgcgacct tccgtggaca ccacgaaggt aacgtcagcg accgcacgac ctcccaacgc
    21301 gggcaaaacg gcatgttgct gcgcgacaac attttcggct ccatcgaaga agacgcccag
    21361 cgccgcgatt tcactatcaa cagcctctat tacagcgtag cggattttac cgtccgtgat
    21421 tacgttggcg gtatgaagga tctgaaggac ggcgttatcc gtctgattgg taacccggaa
    21481 acgcgctacc gtgaagatcc ggtacggatg ctgcgcgcgg tacgttttgc cgccaaattg
    21541 ggtatgcgca tcagcccgga aaccgcagaa ccgatccctc gcctcgctac cctgctgaac
    21601 gatatcccac cggcacgcct gtttgaagaa tcgcttaaac tgctacaagc gggctacggt
    21661 tacgaaacct ataagctgtt gtgtgaatat catctgttcc agccgctgtt cccgaccatt
    21721 acccgctact tcacggaaaa tggcgacagc ccgatggagc ggatcattga acaggtgctg
    21781 aagaataccg atacgcgtat ccataacgat atgcgcgtga acccggcgtt cttgtttgcc
    21841 gccatgttct ggtacccact gctggagacg gcacagaaga tcgcccagga aagcggcctg
    21901 acctatcacg acgctttcgc gctggcgatg aacgacgtgc tggacgaagc ctgccgttca
    21961 ctggcaatcc cgaaacgcct gacgacgtta acccgcgata tctggcagtt gcagttgcgt
    22021 atgtcccgac gtcagggtaa acgcgcatgg aaactgctgg agcatcctaa gttccgtgcg
    22081 gcttatgacc tgttggcctt gcgagctgaa gttgaacgta acgctgaact gcagcgtctg
    22141 gtgaaatggt ggggtgagtt ccaggtttcc gcgccaccag atcaaaaagg gatgcttaac
    22201 gagttggatg aagagccgtc accgcgtcgc cgtactcgtc gtccacgcaa acgcgcaccg
    22261 cgtcgtgagg gtaccgcatg acagtggcgt atattgccat aggcagcaat ctggcctctc
    22321 cgctggagca ggtcaatgct gccctgaaag cattaggcga tatccctgaa agccacattc
    22381 ttgccgtttc ttcgttttac cgcactccac cgctggggcc gcaagatcaa cccgattacc
    22441 tcaacgccgc cgtggcgctt gaaacttcgc tcgccccgga agagttactc aaccatacac
    22501 agcgcataga actgcaacag ggtcgtgttc gtaaagctga acgctgggga ccacgcacgc
    22561 tggatctcga tatcatgctg tttggtaatg aagtgataaa taccgaacgc ctgaccgttc
    22621 cgcactacga tatgaagaat cgtggattta tgctgtggcc gctgtttgag attgcaccgg
    22681 agctggcgtt tcctgatggg gagacgttgc gtgaagtctt acatacaaga gcatttgacg
    22741 aattaagcaa atggtaattt atttatatta cgaatataaa tattttctga aaatcataca
    22801 tcctctcttt ccttaataaa aattttagtt taaacatttt tgcagaaaca taatataaat
    22861 tcccgcagat aaatatcaat cccatcgcca taccctaacc aattgcactt gtgaatttat
    22921 ttttgcttaa ttttgaatta agaaaattta atttgttcaa aagcccttaa agtgcacaat
    22981 aggcgtccta atgaacaatg gttgtaaatt ttggtaacaa atatcattgc tcattaatat
    23041 ctagcatctg cttataacat ttgtgtcatg aatagtcatt cttcaaacca gtagtctgac
    23101 taatggaatt ttattcaagg atttttatgt ctaaaaaatt aggttttgcc ctgtctggtc
    23161 ttatgctggc aatggttgca ggtactgctt ctgctgatat ggatggtggt cagttaaata
    23221 tcagtggtct ggttgttgat aacacctgtg aaacccgcgt tgacggcggt aataaagatg
    23281 gtctgatcct gctgcaaact gcaactgttg gtgaaatcga tgctggcgtg ctgaatgaca
    23341 ccgttggcgc taaagccaaa cctttcagca tcaccgttga ctgcagcaaa gcaaatccga
    23401 atccaggtag tacagctaaa atgaccttcg gttctgtttt ctttggtaat agcaaaggaa
    23461 cgctgaacaa cgacatgtct attaacaacc cgtctgacgg cgtgaatatc gctctgcaca
    23521 atattgatgg ttccactatc aaacaggttc aaattaacaa ccctggcgat gtgtatacta
    23581 aagccctgga tgcaacgaca aaatctgctg tttatgattt taaagcgtct tacgttcgtg
    23641 ccgtcgcaga ccaaacagca actgctggtt atgtaaaaac taacactgca tacaccatta
    23701 cttatcagta agtattcacc aattaaattg attgaatgaa tatacaggga atagtgttaa
    23761 taatttctat ttttatatta ttccctgttt taattaactc tgtcagggat ggtttatgtt
    23821 ttttaacacc aaacatacaa cagctttatg cttcgtaacc tgtatggctt ttagttcatc
    23881 gtctattgcg gacattgtca tttcgggtac tcgcgtaata tataaaagcg atcaaaaaag
    23941 tgtcaacgta cgtctggaaa ataaagggaa taacccgttg cttgtccaga gttggttaga
    24001 tactggcgat aacaacgctg agcctggcag tattacagtc ccttttactg ccacgccgcc
    24061 agtatcgcgt attgatgcca aacgtgggca aacaatcaaa ttaatgtaca cagccagcac
    24121 ctcactgcct aaagacagag agagcgtgtt ctggtttaac gtactggaag ttccaccaaa
    24181 accagatgca gaaaaggtcg cgaatcaaag tctgctgcaa ctggcatttc gcacacgtat
    24241 aaaacttttc tatcgcccgg atggattgaa gggaaatccc tctgaagccc cgttagccct
    24301 gaagtggttc tggtcaggtt cagaaggtaa ggcgtcattg cgagtgacca atccaacccc
    24361 ttactacgtc tcttttagca gtggtgattt agaagctagc ggtaaacgct atccgattga
    24421 tgtgaaaatg attgcaccat ttagtgatga ggtcatgaaa gtcactggcc ttaatggcaa
    24481 agcaagttct gcaaaagtac atttttacgc cattaatgat tttggtggcg ccattgaagg
    24541 taatgccagt ctgtaatcag gcaaggatat aattccgcag gaagcataac gtgactataa
    24601 aaccaactaa aaattatcat aatcatctga cccgtatcgc tacgttttgc gcgctgctgt
    24661 attgcaatag tgctttctgt gctgaacttg ttgaatatga ccataccttc ctgatggggc
    24721 agaatgcctc aaatattgat ctcagccggt atagtgaagg taaccctgct ataccgggta
    24781 tgtacgacgt cagtgtttat gtcaacgatc aacctattat taaccaaagc attacattta
    24841 ttgaaattga aggaaaaaaa acgcacaagc ctgtatcaca cttaagaatt tattgcagtt
    24901 tcatataaat tctcccgata taaataacga aaaagccgtt ctgcttgcca gggatgaaac
    24961 actcggcaat tgccttaatt tgacggaaat tatccctcag gcttctgttc gttatgacgt
    25021 taacgagcaa cgtctggata tagacgttcc tcaggcatgg gtaatgaaaa attaccaaaa
    25081 ctatgttgat ccatcgttat gggaaaatgg cattaatgcg gccatgttgt catacaacct
    25141 caacggatat catagtgaaa ctcctggtcg aagaaatgac agcatttatg ctgcttttaa
    25201 cggtgggatg aatttaggtg catggcgact gcgtgcctca ggcaactaca actggatgac
    25261 cgattctggc agtaattatg attttaagaa tcggtatata cagcgtgata tcgcctcgct
    25321 gcgttctcaa ctcattcttg gtgagtctta tacaacgggc gaaacctttg attctgtcag
    25381 tatccgcggc attcgtttat acagcgacag ccgcatgttg cctccgacat tagcgagttt
    25441 tgcgcctatc attcatggcg ttgctaatac caacgccaaa gtcactatta cgcaaggtgg
    25501 ctataagatt tatgaaacga ctgtgccgcc aggcgctttc gtcattgatg atctgagtcc
    25561 gtcaggttac ggcagcgatc ttattgttac tgtcgaagaa tccgatggct caaagcggac
    25621 attctcgcaa cctttttcat cggtcgttca aatgttacgc cctggcgttg gacgttggga
    25681 tattagcggc ggccaggtct taaaagatga tattcaggat gagcctaatt tatttcaggc
    25741 cagctactac tacggtctga ataactatct aac
//
LOCUS       DAETQN010000053        25647 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.053, whole genome shotgun sequence.
ACCESSION   DAETQN010000053 DAETQN010000000
VERSION     DAETQN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.053"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(76..807)
                     /gene="rlmB"
                     /locus_tag="K0W68_002182"
     CDS             complement(76..807)
                     /gene="rlmB"
                     /locus_tag="K0W68_002182"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBI3073734.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(987..3428)
                     /gene="rnr"
                     /locus_tag="K0W68_002183"
     CDS             complement(987..3428)
                     /gene="rnr"
                     /locus_tag="K0W68_002183"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBI3073735.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="K0W68_002184"
     CDS             complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="K0W68_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBI3073736.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4097..5395)
                     /gene="purA"
                     /locus_tag="K0W68_002185"
     CDS             complement(4097..5395)
                     /gene="purA"
                     /locus_tag="K0W68_002185"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBI3073737.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5499..5696)
                     /locus_tag="K0W68_002186"
     CDS             complement(5499..5696)
                     /locus_tag="K0W68_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBI3073738.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="K0W68_002187"
     CDS             complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="K0W68_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBI3073739.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="K0W68_002188"
     CDS             complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="K0W68_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBI3073740.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="K0W68_002189"
     CDS             complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="K0W68_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBI3073741.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="K0W68_002190"
     CDS             complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="K0W68_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBI3073742.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="K0W68_002191"
     CDS             complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="K0W68_002191"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBI3073743.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="K0W68_002192"
     CDS             complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="K0W68_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBI3073744.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="K0W68_002193"
     CDS             complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="K0W68_002193"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBI3073745.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="K0W68_002194"
     CDS             complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="K0W68_002194"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBI3073746.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="K0W68_002195"
     CDS             complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="K0W68_002195"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBI3073747.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16016..17155
                     /gene="queG"
                     /locus_tag="K0W68_002196"
     CDS             16016..17155
                     /gene="queG"
                     /locus_tag="K0W68_002196"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBI3073748.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17138..17191)
                     /locus_tag="K0W68_002197"
     CDS             complement(17138..17191)
                     /locus_tag="K0W68_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073749.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17425..17500)
                     /locus_tag="K0W68_002198"
     tRNA            complement(17425..17500)
                     /locus_tag="K0W68_002198"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17465..17467),aa:Gly,seq:gcc)
     gene            complement(17536..17611)
                     /locus_tag="K0W68_002199"
     tRNA            complement(17536..17611)
                     /locus_tag="K0W68_002199"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17576..17578),aa:Gly,seq:gcc)
     gene            complement(17769..17844)
                     /locus_tag="K0W68_002200"
     tRNA            complement(17769..17844)
                     /locus_tag="K0W68_002200"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17809..17811),aa:Gly,seq:gcc)
     gene            complement(18055..18600)
                     /gene="orn"
                     /locus_tag="K0W68_002201"
     CDS             complement(18055..18600)
                     /gene="orn"
                     /locus_tag="K0W68_002201"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBI3073750.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18695..19747
                     /gene="rsgA"
                     /locus_tag="K0W68_002202"
     CDS             18695..19747
                     /gene="rsgA"
                     /locus_tag="K0W68_002202"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBI3073751.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19844..20812
                     /gene="psd"
                     /locus_tag="K0W68_002203"
     CDS             19844..20812
                     /gene="psd"
                     /locus_tag="K0W68_002203"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBI3073752.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20834..24157
                     /gene="mscM"
                     /locus_tag="K0W68_002204"
     CDS             20834..24157
                     /gene="mscM"
                     /locus_tag="K0W68_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBI3073753.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24308..>25647)
                     /gene="yjeM"
                     /locus_tag="K0W68_002205"
     CDS             complement(24308..>25647)
                     /gene="yjeM"
                     /locus_tag="K0W68_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBI3073754.1"
                     /translation="MMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWM
                     VSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIA
                     RITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVF
                     AIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLS
                     NGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTL
                     CYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAF
                     FNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVTF
                     ANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5934 a         7194 c         6499 g         6020 t
ORIGIN      
        1 tcaccgtaat tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt
       61 cataattcag acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc
      121 aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca
      181 atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaagcg
      241 tccggtcatt ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca
      301 gatattctct tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg
      361 aacgctttct gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg
      421 atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc
      481 gaggttgtgc ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc
      541 gatcagatcc ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat
      601 gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat
      661 aaccacgccc tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc
      721 ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc
      781 gtggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc
      841 actacgttta tccggcctac atgatctctg caatatattg aatttgcgtg attatgtagg
      901 ccggataagg cgttcacgcc gcatccggca tgaacaaagc gcattttgtc agcaatctaa
      961 ccctcttctt ttaaagaggg tattgatcac tctgccactt ttttcttcgc cgcacgcttc
     1021 gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg
     1081 tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct gtctggctca
     1141 aagtttacct ttttaccgac ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt
     1201 ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga
     1261 ctaaagtcga ttttgcgctc gtccatatta accgcttcga cgcgaacttc cacgcgatcg
     1321 cccaggcgat aagtctggcc gctggattcc cccatcaggc gttgccctac ctggtcaaag
     1381 cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc
     1441 agacggacga agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct
     1501 acctggtcga gcatgaagtc acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg
     1561 gcacgacgtt ccgccatcga acagtgctga cccagttgca acatctcttc catcgaataa
     1621 tggtagccgc cggtttcagt ggtgttgccc tgatgcccct gctctttcgc cagcagatac
     1681 ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca
     1741 taggactgca atgccaggcc aaagtgacca cggttttctg gatcgtaaat cgcctgtttc
     1801 atcgagcgca gcagcatggt ttgcagcatt tctgcatcag gacggtcggc aaccgactcc
     1861 agcagttccg cgtagtcacg cggttccggc ttgttaccac ccggcagctc cagccccagc
     1921 tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg
     1981 aacagtgccg gttctttcgc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc
     2041 atgcactctt caattaattt gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata
     2101 cggcgttcag cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt
     2161 tcttcacggg ctttatccag cactttatag aggttatgca actcttcgag atgcttaacc
     2221 agcgggacgt actgctcgcg cagatcctga tcgccctgca gaatatgcca gactttggtg
     2281 taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg
     2341 cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc
     2401 gaacacaggc cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac
     2461 accgacgtgc cacggttacg cgcttctctg tccagcggcg ttggcggacg cacatagtag
     2521 ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa
     2581 actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc
     2641 aaatcgacac ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc cgcaacctgt
     2701 tgctcaacag cctgcggcca gatatacgga atttcatggg tacgcagagc gatatcaacc
     2761 gccatgccgg tgcccatatt gtcgcccagc acttcgacga ttttacccac cgctttggtg
     2821 cggcgagtcg gacgctgagt cagttcgact acgaccacaa agcccatccg cgcgcccatg
     2881 atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg aaccacaaag
     2941 ccgacgcccg cttcggtaaa gtagcgacca acaatctggc tggtttttgg caccagtacg
     3001 cggacaatac gcgcttcacg acgaccttta cggtcagcac ccagcggctg cgccagcacc
     3061 tgatcgccat gaatacaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc
     3121 ccttcaaccc gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg
     3181 tcgaggcgtt ccggcagcgc atagcactga cgacgagtga agaccagttg accatcgcgc
     3241 tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt
     3301 tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg ttcgaggata
     3361 aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct
     3421 tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca
     3481 ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt
     3541 ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa
     3601 tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat
     3661 caccaatacg tatcgcactc gccggtttac ccaggcgaat gccgccattt tttccacgaa
     3721 cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg
     3781 agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg
     3841 ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct
     3901 caaaagggaa atcgggaaaa aatacattta tattgatgat aaaccagcca caagctgtgt
     3961 cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag
     4021 cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct
     4081 gccaggcgta ccagaattac gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat
     4141 ccggaccggt agagatgata tcgatcggca caccggtcag ctcttcaata cgcttgatgt
     4201 agttcagcgc cgcctgcggc aggccgctac gatctttcac gccgaaagtg gattcagacc
     4261 agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag
     4321 tggtagccac ttcgcgacca tccggcatac ggtaagccac gcagagttta acctctttca
     4381 ggccatccag aacgtccagc ttggtcaggc aaaagccaga cagggagttc agctgtaccg
     4441 cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc gtagttgcgc
     4501 cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga
     4561 acggacctgc acctacacga gttgagtaag ctttgaggat acccagaaca taatcaacat
     4621 aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta
     4681 cgtacggata agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga
     4741 cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc accatagaag
     4801 tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag
     4861 ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag
     4921 cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgct actttatctt
     4981 cataagcagg cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct
     5041 cacgcgcgtt atccagcgca acgtgataat caaggatcag cggacatgct tcagacagca
     5101 gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg
     5161 cggccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac
     5221 ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt
     5281 tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga tcgacgatct
     5341 tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc
     5401 aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt
     5461 ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac
     5521 atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta
     5581 tcgggcaaat tggtcatcgc agagatcatc ttcttccatg ccttcgggta aagcatcggc
     5641 cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga attcattcat
     5701 tatccttata gaaaaagaaa accaccgaca tccctgagga tgcggtggct ttattgacct
     5761 gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa
     5821 tcgctatccg ggctcatgac catcacgtcc tgattgccag agaagctgtt ctcataagca
     5881 cgcaggctac ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc agcaaacagt
     5941 ttggctgctt cggcatcgcc ttcaccacgc atgatgcggc cctgacgctc agcttctgcc
     6001 agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct
     6061 tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt gtagatcgct
     6121 tcagacactt cggtcggcag gttgatctgc ttgatacgca catcgacaac ttcaataccc
     6181 agcgccgcca tactgttcgg gttgatgacc ggaactttgc ccttcgtctc agccgttacg
     6241 cgctctgccg cttcggcaat ggcgttatct gccgccgggg tagtaacttc atcttctgta
     6301 cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc acgggaatcg
     6361 gtgacgatgt ctttcacgtc caggcgacca atttcagaac gcagacggtc agagaactta
     6421 cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag gtagtaacgg
     6481 ctgaaatcgc tgatgcgcca tttgatgtaa gaatcgacga tcaggtcttt cttctctttg
     6541 gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag cattttcacc
     6601 gtttcaatga acggtatctt gaaatgcaga cccggctcat aaaccagagg tttgttgtca
     6661 tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc tttgacgaca
     6721 aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac tgacttacgc
     6781 atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca
     6841 taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg gaggaagagg
     6901 ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca ggggcgttac
     6961 cgcctttcag catctggtct aacggcagaa ccatcaggtt gccaccttta tcgttaacca
     7021 gcactttgcg ggtgttaccc aacacttttt ccatcgtctc gatatacaga cgctcgcgag
     7081 taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc acttcaccct
     7141 gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct
     7201 gaccgttcgc acgcggctga acttcgttgg tatacgcttc tgcttcacga atgtattgct
     7261 gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct tccggcggac
     7321 gagctgcctg gaagttgacg tccagcagag tgatacccat gtcatacgga cggatagtct
     7381 cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc agaatgcggt
     7441 ccatggtgta tttaccgata actccacgca gggcgctgtc ggtagcctga cgcaggctgt
     7501 catccgggct ggtcacgcta tacagatatt tttccggatt ggtgacgcgg tactgcacgt
     7561 tcatctcaac gcgcactacg ttctcgtccg acgtcagcat cacaccagaa gcggccagtt
     7621 cacgcacggc ttccacgttc accggtttga cttcgtcgat aaacgtcggt ttccagttca
     7681 gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgccg cgttcggctt
     7741 ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc gctgcgatgg
     7801 taacgacacg accgccaagc tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc
     7861 cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg aagatatcat
     7921 ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca gagttgccgc
     7981 caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc tgattccacg
     8041 ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga cgccagagga
     8101 tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa cgccggttct
     8161 tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact tacgctgccg
     8221 tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact tctcagacgt
     8281 ccttcctgcg gcggcaaacg caatgtatgc tgcgccacct cgccggaaag ccgctccgtc
     8341 aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg cggaaagcca gacacggatc
     8401 ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat cttccagcat atcgatcttg
     8461 ttcatcacca gcagggttgg gatctcgtga gcgtcgatct cttcaagaac cgtattcacc
     8521 gcttcgatgt tttcttgtac acgtacatcc gccgcatcaa tgacgtgcag cagtaatgtg
     8581 gcttgccgcg tctcttgtaa cgtggcttta aatgctgcca ccagatcgtg cggcaggtgg
     8641 cgaataaacc ctacggtatc tgcaagtacg gtttcaccga catccgcgac gtcaatacgc
     8701 cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg
     8761 atgcgattga aaagggtaga tttaccggcg ttggtatatc ccaccagcga aacagtagga
     8821 acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac tctttctagg
     8881 cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc gagctgggtt
     8941 tcacccggac cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt ccagccacgc
     9001 accaggcgcg tagccagatg gcgcagctgc gctagctcaa cctgcaactt accctcatgg
     9061 gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat aacacgacac
     9121 tcgcacaaac gctccaggtt acgctcttgc gccgggctca gggcatggtc aaaaagaacg
     9181 accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa ctgctttacc ttcacctaca
     9241 aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc ttcgacaccg
     9301 gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa
     9361 tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa gcgtataacc
     9421 ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc
     9481 gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc
     9541 atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg
     9601 agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa
     9661 caggatcacg aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa
     9721 ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga
     9781 ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat
     9841 tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa
     9901 caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc
     9961 cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac
    10021 ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat
    10081 aacccacgca acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc
    10141 ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat
    10201 ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt
    10261 ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg
    10321 cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct
    10381 cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg
    10441 cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat
    10501 acaacatcgt accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt
    10561 ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat
    10621 ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat
    10681 ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac
    10741 gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct
    10801 taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa
    10861 tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc
    10921 acgtccgcca gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga
    10981 tttcgtgcaa tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag
    11041 cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc
    11101 ctgatggtca catgctgtgc atctgactgg aaatcaatac ccaattccgc cagggcagac
    11161 tgcgcttttt ctaatgccga tttttcttcg ccagaaactt ttagccgcaa cggaataagc
    11221 agcggctgag cgcaaactgg cgcttcaccc ggcgtcagtt gtacctgacg cagccaacgt
    11281 tctgccactg gcaaggctaa aagtgaaatg ttgccgtcgc gctccagcaa cgcacagtcg
    11341 gaatggacga tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc
    11401 tgcggttccg gcgcttttaa tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac
    11461 acttcacctt gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga
    11521 ctgcctgatg ctggcgcagg agtgtagcgc ggagctaccg gctcacgagc tgccggttct
    11581 gcaaagtgat tgcgccccgc cgccacgcgg ttttccggaa tggaacgcgg tgcaggttgg
    11641 ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc
    11701 tgatagataa aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg
    11761 tgcacgttga cgtccacctg atgcggatcg atctccagat acagcacaaa tgccggttgc
    11821 tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg
    11881 cgcatcatgc gaccgttcac gtagcaatac tgaatttctg ccagtgcggg cgtggtgtga
    11941 tttggatcgg ccacccagcc gcgtagcgtg agatcgccgt gttgccattc aatcgccagc
    12001 gcttgttcaa gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct
    12061 tccggcactg cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg
    12121 tcgaaacgcg ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa ttcggttttc
    12181 tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc
    12241 gtcgtcccca caggatgcgc cgccggtttt accgtcacgt tcatatcgcg cccttcggca
    12301 taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc
    12361 gaactgatac tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg
    12421 tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt
    12481 ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca
    12541 atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac
    12601 gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc
    12661 tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg
    12721 gcagtttgtg ccgttgcccc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg
    12781 cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa
    12841 cgttcttcgc tgttgttgct gataaaaccg gtttcgacca gtactgacgg gatatccggc
    12901 gaacgcagaa cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt
    12961 tgcaactgac tgatcatact ggtcgctaca tcatacccta cccgctggga atgaccgaac
    13021 tgtaaatcca gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatcg
    13081 cccgccccac ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttcg
    13141 ctgttggcgc gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt
    13201 gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcgctg
    13261 cgccccatca ccgagataaa gtaatccccg tcacgggtta aaacgccttt aaacatcgga
    13321 tcgtcattga gcaaagtgcg caatttacgc gcgatggcga tggtgacatt tttctcccgc
    13381 gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg
    13441 ataattttat cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg
    13501 ctgataacac ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg
    13561 acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggtggagga
    13621 ggcggtggcg gcacatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc
    13681 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
    13741 gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac
    13801 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
    13861 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta
    13921 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc
    13981 agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
    14041 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
    14101 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
    14161 ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc
    14221 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa
    14281 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
    14341 cccagagctt gtaaaaaacc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
    14401 agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
    14461 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
    14521 gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa
    14581 acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac aggcggcatc
    14641 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
    14701 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
    14761 ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg
    14821 tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
    14881 ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
    14941 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
    15001 tgcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
    15061 ttccaggctt tcggtaagag agtccatcgt cagttcatgc accatcaatt ccggtcgtgc
    15121 agtcagcagc ggcgcaatgt tttcactgcg ggtcagtact cggactaaac cagcaccagc
    15181 acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat
    15241 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg
    15301 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
    15361 cagccccagt gagtcaaaat gcagttgtcc tgtaacatcc cgcgcttttc cagtaagcaa
    15421 gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt
    15481 agcgccggtt tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg
    15541 ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
    15601 accgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
    15661 gatctcgcca cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
    15721 tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc
    15781 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
    15841 gtcaggatac gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc
    15901 atagagtgtg agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
    15961 gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
    16021 agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg
    16081 ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc
    16141 atggctggac aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg
    16201 cgctcgcccc catgagttat taccaggtac gctgcgtgta atcagcgtgc ggatgaatta
    16261 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
    16321 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg
    16381 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
    16441 tattctcgag cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact
    16501 tatcctcaat cgcgaggccg gttctttctt ctttttaggc gaattgctgg tcgatattcc
    16561 gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcatagcct gtatgacgat
    16621 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
    16681 tctcaccatc gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg
    16741 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
    16801 tacagaagac gatttcagcc cgcgtaagcc gctacacgca caggaactca ttgagttatt
    16861 tgcctggagc gaagagaagt ttttaaaagt cacggaagga tcggcgattc gccgtatagg
    16921 tcacctgcgc tggctgcgta atatcgccgt ggcattaggc aatgcgccct gggatgaaac
    16981 gattctggcg gcgctggaga gtcgcaaagg tgagcaccca cttcttgatg agcacatagc
    17041 gtgggcgatt gcgcagcaaa tcgagagacg aaatgcgtgc atagtcgaag tgcaattgcc
    17101 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
    17161 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgcataa aatcgtcaag
    17221 tgatcgactc gacaaaacgg aatcttgatt tttaacttat ttatcaatag gttaaaaaaa
    17281 taccattcac cgtgcgaagt ttctttgaaa ttaagattac agcctgagga tgtggataac
    17341 tctgtttaca agagtttgtc agagacaaca aaaaccagca ataacgcatc gcgtcgctgt
    17401 ggatatttta ttgagagaag aatttggagc gggaaacgag actcgaactc gcgaccccga
    17461 ccttggcaag gtcgtgctct accaactgag ctattcccgc ttgggtggtc tgtgccttac
    17521 agcactttca aattttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa
    17581 ggtcgtgctc taccaactga gctattcccg cttaatcttc gtctttcgaa ccgctactgc
    17641 cttggctgcc ttcactcacc ttagtcacat acttttgtaa gttcccaagg attcgttcag
    17701 ttgccgcctt gtcgcaattc gaaatactgc gattgggtgg tctgtgcttt gcagcacttt
    17761 tcaaattttg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg
    17821 ctctaccaac tgagctattc ccgcgtacta cttaattttg ctttcgtaat tttcgcattt
    17881 ctgcgtcgtt acgggaggcg cattatacga gaaatctttc cctctgcaac cccccaaaaa
    17941 agtttttttt gcgtttttat ctcaagtgct gaaattatca gcaagacgtt caatttagcg
    18001 tcaaaatcac gccgccgcaa gtggattccg gcgcttacgt gaccaggaaa aattttacag
    18061 cttgataaaa tgctcgcggt agtaagccag ctccgccacc gattcacgga tatcatccat
    18121 tgcctgatgc gtcccctgct tggtaaaacc atccagaatt tccggcttcc agcggcgcgc
    18181 cagctctttc agggtgctga catcgagata acggtagtgg aagtaggctt ccagctccgg
    18241 catgtattta aacaggaaac gacggtcctg tccgatgctg ttaccgcaaa tcggcgattt
    18301 tcccgcaggc acccactgtt ttaagaattc gagcgttgcc agttcagctt cccgatcgcc
    18361 catcgtgctc gctttcacgc gctctaccag cccgctggcg gtatgggtgc gcacgttcca
    18421 gtcatccatc agcgccagct gttcatcgga ctgatgtact gcaatggtcg gcccttctgc
    18481 cagaatattc agattggcat cggtcaccag cgtggcaatc tcaataatgc gatcgcgctc
    18541 gggatccaga ccggtcatct caagatcgat ccaaatcagg ttgttttcat tggcactcat
    18601 gctattttcc acccattcac agtgactata ttgatccaca attgcgtgta tcatagatgt
    18661 tttgcccatc aggggcgacc aggagtcagt acgattgagt aaaaataaac tctccaaagg
    18721 ccagcagcgc cgcgtgaacg ccaatcacca gcgtcgtctt aaaacgtcta aggagaagcc
    18781 cgactacgac gacaatctgt ttggcgagcc tgatgaaggt atcgtcatca gccgctttgg
    18841 tatgcacgct gatgtggaat ccgccgatgg cgacgttcac cgctgcaata ttcgccgtac
    18901 catccgttcg ctggtaaccg gcgaccgcgt agtctggcgt ccgggtaaac cggcggcgga
    18961 aggcgtaaat gtcaaaggga tcgtggaagc ggtgcatgag cgtacctcgg tgttgacgcg
    19021 tccggatttc tacgacggcg taaaacctat tgccgccaac atcgaccaga ttgtcattgt
    19081 ctccgccatt ttgccggagc tgtcgctcaa tattatcgac cgttacctag tggcctgcga
    19141 aaccttgcag attgagccga ttattgtgct caacaagata gacctgctgg acgacgaagg
    19201 catggcgttc gtcaacgagc agatggatat ctaccgcaat atcggttatc gcgtattgat
    19261 ggtttccagc catactcagg atgggctaaa accgctggaa gaggcgttga ccgggcgcat
    19321 cagcattttt gccgggcagt ctggcgtcgg caaatccagc ctgctgaacg cgttgctggg
    19381 gctgcaaaaa gagatcctga ccaacgatgt ctctgacaac tcggggcttg gtcagcacac
    19441 cacgaccgcc gctcggctgt atcacttccc gcacggcggt gatgtgattg attcccctgg
    19501 tgtgcgtgag ttcggcctct ggcacctgga gccggaacaa atcactcagg gctttgtcga
    19561 attccatgac tatttaggtc tgtgtaaata tcgcgattgc aaacacgata ccgatccggg
    19621 ctgcgctatc cgggaagcgg ttgaggaagg gaaaatcgcg gaaacccgtt tcgaaaacta
    19681 tcaccgtatt ctggaaagca tggcgcaggt aaaaacgcgt aaaaactttt ctgatacgga
    19741 tgactgacaa ttaagctaac cgtcgttaga atcgtcccct ttttttcagg atccggcatg
    19801 taggccgaat caggaacgac aaaacaatgg cctggaggct accttgttaa attcatttaa
    19861 actttcgcta cagtacattc tgccgaaact atggcttact cgcctggcgg gttggggcgc
    19921 aagcaagcgg gcaggatggt tgacaaaact ggttatcgat ctgttcgtta aatactacaa
    19981 ggtcgacatg aaagaggcgc aaaagccgga caccgccagc taccgcacct ttaacgaatt
    20041 ctttgtccgt ccgctgcgtg acgaagtacg cccaatcgat accgatccga atgtactggt
    20101 catgcctgcc gatggcgtta tcagccagtt gggtaaaatc gaagaagata aaatcctgca
    20161 agccaaaggc cacaactaca gcctcgaagc cctgctggca ggcaactatc tgatggcgga
    20221 cctgttccgc aacggtacgt ttgtgaccac ttacctctcc ccgcgtgact accaccgcgt
    20281 acacatgccg tgcaacggta ttctgcgtga gatgatctac gtgccgggcg atctcttctc
    20341 cgttaaccat ctcacggctc agaacgtgcc gaatctgttt gcccgtaacg aacgcgtgat
    20401 ttgccttttc gataccgaat ttggcccaat ggcgcagatt ctggtcggag cgacgattgt
    20461 tggcagcatt gagacggtct gggcgggcac cattacgccg ccgcgcgaag gtatcatcaa
    20521 gcgctggacc tggcctgccg gggaaaacga cggttctgtg gcactgctga aaggccagga
    20581 aatgggtcgc tttaaactcg gttccaccgt tatcaacctg tttgcaccgg gtaaagtgaa
    20641 tctggttgag caactggaaa gcctgtctgt cacgaaaatt tgccagccgc tggcagtatc
    20701 taccgaaacc tttgttacgc cagacgctga acctgccccg cttcctgctg aagagatcga
    20761 agcagaacac gacgccagcc cattggttga cgacaaaaaa gaccaggtct aatccatcaa
    20821 aggaaacgct gacgtgcgcc tgattatcac ttttctgatg gcctggtgcc tcagttgggg
    20881 ggcgtacgcc gcgacggccc ccgatagcaa acaaatcact caggaactgg agcaggcaaa
    20941 agcggcgaaa cccgcacagc cggaagtcgt agaggcgctt cagtctgcct taaatgcgct
    21001 tgaggaacga aaaggttccc ttgagcgcat caaacaatat cagcaagtta tcgataatta
    21061 tccgaaactc tccgctactc tgcgcgcaca attaaacaac atgcgtgaca agccgcgcag
    21121 cgtgtcgccg ggaatgtcta ccgacgcgct gaatcaggaa attctccagg tcagcagcca
    21181 gttgctggat aaaagccgtc aggcccagca agagcaggag cgcgcccgcg agattgccga
    21241 ttcgctgaat caactgccgc aacagcaaac cgacgcccgc cgtcagttaa atgagatcga
    21301 gcgccgcctg ggaacgctta ccggcaatac tccgctcaat caggcacaaa atttcgcgtt
    21361 gcagtctgac tctgcacgtc ttaaggcgct cgttgatgaa ctggagctgg cgcagctgtc
    21421 tgccaataac cgccaagaat tagcgcgctt acgctcagag cttgcggaaa aagagagcca
    21481 gcaactggat gcgtatttgc aggccttacg taatcagttg aatagccaac gtcagcttga
    21541 ggcggagcgg gcgctggaaa gtaccgaatt actggcagaa aacagtgccg atttgccgaa
    21601 agatatcgtc gcgcaattca aaattaaccg cgaactatcg gcagcgctga atcaacaggc
    21661 gcagcggatg gatctcgttg cctcgcaaca gcgtcaggct gccagccaga cgttacaggt
    21721 ccggcaggcg ttgaatacgc tgcgtgaaca gtcgcaatgg ctgggatcgt ccaatctgct
    21781 cggtgaagcg ttgcgagcgc aggtggcacg gctaccagaa atgccaaaac cacaacagct
    21841 tgataccgaa atggcgcagt tgcgtgtgca acggttacgt tatgaggatc tgcttaataa
    21901 acagccgctg ctacggcaaa tccatcaggc cgacggtcag ccgctgaccg ccgagcaaaa
    21961 ccgtattctg gaagcacaac tgcgcactca gcgtgagttg ctgaactcat tgttgcaggg
    22021 tggcgacacg ctactattgg aactgaccaa gctgaaagtc tccaacgggc aactggagga
    22081 tgcgctgaaa gaggtgaacg aagcaacgca ccgctatctg ttctggacct ctgacgtgcg
    22141 tccgatgacc atcgcctggc cgctggaaat cgcccaggat ctgcgtcgtc tcatttcgct
    22201 ggacaccttc agtcagttgg gcaaagccag tgtgatgatg ctgaccagca aagagacaat
    22261 tttgccgctg tttggcgcgt tgattctggt cggttgcagt atttactcgc gccgctattt
    22321 cacccgtttt cttgaacgtt cggcggcgaa agttggcaaa gtgactcagg atcacttctg
    22381 gctgacgttg cgcactcttt tctggtcgat tctcgtcgcg tcaccgttac cggtgctgtg
    22441 gatgacgctg ggttacggct tgcgcgaggc gtggccttat ccgctggcgg tcgcgattgg
    22501 cgatggtgta acggccaccg tgccgctgct gtgggtagtg atgatttgcg ccacctttgc
    22561 ccgcccgaac ggcttgttta tcgctcattt tggctggccg cgcgaacgcg tatctcgtgg
    22621 gatgcgctac tacctgatga gcatcgggct tattgtgccg ctgattatgg cgctgatgat
    22681 gttcgataac ctcgacgacc gtgaattctc cggttcgctg ggaaggcttt gctttatcct
    22741 catttgcggt gcgctggcgg tggtcaccct cagcctgaaa aaggccggga ttccgctgta
    22801 tctcaacaaa gaaggcagcg gcgacaacat taccaaccat atgctgtgga acatgatgat
    22861 tggcgcgccg ttggttgcca ttctggcgtc ggctgtgggt tatctggcaa cggcacaggc
    22921 gctgttagcg aggcttgaaa cctcggttgc catctggttc ctgctactgg tggtttatca
    22981 cgttatccgc cgctggatgc tgatccagcg ccgcaggctg gcgtttgacc gggcgaagca
    23041 tcgccgggca gagatgttag cgcaacgtgc gcgtggcgaa gaggaagcgc atcatcacag
    23101 tagcccggaa ggagcaattg aagtcgatga aagcgaagtc gatctcgatg ccatcagtgc
    23161 gcaatccttg cggctggtgc gctcaatttt gatgttgatc gccctgcttt ctgtcattgt
    23221 gctgtggtca gaaatccatt ccgctttcgg cttcctcgaa aatatttcgc tgtgggatgt
    23281 cacctccacg gtacagggcg tagaaagtct ggagccaatt accctcggtg cggtgctgat
    23341 tgccattctg gtgtttatca tcaccacgca gctggtgcgc aacctgcccg cgctgctgga
    23401 actggcgatt ttgcagcacc tggatttaac gccgggtacg ggctacgcca tcaccaccat
    23461 caccaaatat ctgctgatgc tgattggcgg gctggtcggc ttctcgatga ttggtattga
    23521 gtggtcgaaa ttgcagtggc tggtcgccgc gctcggtgtt ggtctcggtt ttggtttgca
    23581 ggaaattttc gccaacttta tctctggtct gattatcctg ttcgaaaaac cgattcgcat
    23641 tggcgatacg gtgacaattc gcgatctcac cggtagcgtg acgaaaatta acacccgcgc
    23701 caccaccatc agcgactggg accgtaaaga gataatcgtg ccgaacaagg cgtttattac
    23761 cgagcagttt atcaactggt cgctctctga ctcggtcacg cgcgtagtgt tgacgattcc
    23821 ggcccctgct gatgccaaca gtgaagaagt gacggaaatc ctgctcaccg cagcgcgtcg
    23881 ctgctcgctg gtgatcgaca acccggcacc ggaagtcttc ctggtggatc tgcaacaagg
    23941 gattcagatt ttcgagctgc gtatttatgc cgctgagatg ggtcaccgta tgccgctacg
    24001 ccatgagatc caccagctga ttctggctgg cttccatgcc cacggtatcg atatgccatt
    24061 cccgcccttc cagatgcgtc tggaaagcct caacggtaaa caaacgggga gaacgctgac
    24121 gtctgcgggc aaaggtcgtc aggcgggaag tttgtaatgc atgaagctgc caaatgatgt
    24181 caacgtggct catttagcag cttaacatca tcttcagtta tcaccggccc tggagagtat
    24241 catccatacg cattacttcc tgacaactca tcgccggaga gacgatgagt tgcaggattt
    24301 catatgatca gtcgagagct aactcaggtt tattcgccat gcgactgcaa tagttctggt
    24361 aaatcgccat cgctaacagc gagaagaaga cagggccgcc aatcatccac aatgtgctgt
    24421 cccagtctcc ggcttcaacc acaggctgaa taatggtgaa gacgttcgca aatgtcacca
    24481 ccagtacgac aaccaccatc gcaatcattg ccgacatacg cgttttgaaa atcacaaatg
    24541 gtctgtcgag atcctgacgt gctttaaaga atgggaaagc cagcgcgagg aacaggtaag
    24601 gaagcgtcat agacacgttc gccatcagcg tcagcttgtt aaagaacgcg gatgcggtac
    24661 cgccaccaaa cgaaaccagc aggatgaaga cagtaaccaa cccgcactgc atccacatgg
    24721 cgatagacgg catccccatc gcattcaggc gcgtcatcgg ttccggccac aacgctttcg
    24781 gcgtcccctg gatgatggct ttcagcggtg aatagcaaag cgtaaagaac gcaccggtat
    24841 aagcgaggaa catcgaaagt ccggtaatac gcgcaaacca tacgcccagc gacaatgacg
    24901 cttcaggtga caaatgcagt gcgttaccca gcgtcacccc gaggctcttc atcagcacat
    24961 aggtaatatt gccaaggtta acggaaccat tacttaatac ctgctgccag tttgtgctga
    25021 cgccccataa aaatattgcc agcgaataac cgattgaaat aacaatagcg gcaaaaacaa
    25081 tacctttggc aaagttcttt tctggatttt ccgttttatc gaccagacca ccaaccgctt
    25141 caattccgcc ataggcaaaa atagcaaata ccacaaacga tagcattgcc agaccggact
    25201 gataacccgg gttcggtgat gcaaggaaat taatatcctg cgcgaaatgc ccaccattta
    25261 ataacaaaat agtaatgctt actaacagca atactaaatt cagacacatt actgcaatac
    25321 cgcccaccgc agtaatgcgg gcaattttat taatcccctt tgaagcaacg acggtgacca
    25381 gaatcatcca tgctactgcc agcagaccaa ccacctgcgt aggctccagt ccggcaatac
    25441 gccagtgctg ggtcatgtcg ctaccataga ggaatgttga gaacggtacc caaactttcg
    25501 cggaggtact caccatccag atgatataag aggaaaacca cataaacgta ccaatgaagg
    25561 caaaacgtgg tccgacacta ttattcatcc aggaatagat accaccttct tctttgcgat
    25621 aagcagctcc catttcagcc atcatta
//
LOCUS       DAETQN010000054        25602 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.054, whole genome shotgun sequence.
ACCESSION   DAETQN010000054 DAETQN010000000
VERSION     DAETQN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25602
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.054"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1843)
                     /gene="traD"
                     /locus_tag="K0W68_002206"
     CDS             complement(<1..1843)
                     /gene="traD"
                     /locus_tag="K0W68_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HBI3073755.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIAEFAAGEIGEKEHLKASLQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVT"
     gene            complement(1894..2631)
                     /locus_tag="K0W68_002207"
     CDS             complement(1894..2631)
                     /locus_tag="K0W68_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073756.1"
                     /translation="MTSDNIAEYIGENNQFTEKPATEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(2834..3565)
                     /locus_tag="K0W68_002208"
     CDS             complement(2834..3565)
                     /locus_tag="K0W68_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HBI3073757.1"
                     /translation="MKTKKMMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDAAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3579..4088)
                     /gene="traS"
                     /locus_tag="K0W68_002209"
     CDS             complement(3579..4088)
                     /gene="traS"
                     /locus_tag="K0W68_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HBI3073758.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITLFVIISFIYTIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            complement(4085..6910)
                     /gene="traG"
                     /locus_tag="K0W68_002210"
     CDS             complement(4085..6910)
                     /gene="traG"
                     /locus_tag="K0W68_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HBI3073759.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAERRRAMAWFFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            complement(6907..8280)
                     /locus_tag="K0W68_002211"
     CDS             complement(6907..8280)
                     /locus_tag="K0W68_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HBI3073760.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMNYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            complement(8267..8692)
                     /gene="trbF"
                     /locus_tag="K0W68_002212"
     CDS             complement(8267..8692)
                     /gene="trbF"
                     /locus_tag="K0W68_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HBI3073761.1"
                     /translation="MNHQKYQRELMMKEKINDTEPGIKQIEREIERGCDNAKKYFWLF
                     VVFFAAGLIVRNVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLA
                     AASLSPLCEIIFGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            complement(8640..8987)
                     /gene="trbJ"
                     /locus_tag="K0W68_002213"
     CDS             complement(8640..8987)
                     /gene="trbJ"
                     /locus_tag="K0W68_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HBI3073762.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            complement(8917..9462)
                     /gene="trbB"
                     /locus_tag="K0W68_002214"
     CDS             complement(8917..9462)
                     /gene="trbB"
                     /locus_tag="K0W68_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HBI3073763.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            complement(9449..9733)
                     /gene="traQ"
                     /locus_tag="K0W68_002215"
     CDS             complement(9449..9733)
                     /gene="traQ"
                     /locus_tag="K0W68_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HBI3073764.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            9814..10149
                     /locus_tag="K0W68_002216"
     CDS             9814..10149
                     /locus_tag="K0W68_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HBI3073765.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYIYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            complement(10130..10471)
                     /gene="trbA"
                     /locus_tag="K0W68_002217"
     CDS             complement(10130..10471)
                     /gene="trbA"
                     /locus_tag="K0W68_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HBI3073766.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(10485..11228)
                     /gene="traF"
                     /locus_tag="K0W68_002218"
     CDS             complement(10485..11228)
                     /gene="traF"
                     /locus_tag="K0W68_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HBI3073767.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(11221..11478)
                     /gene="trbE"
                     /locus_tag="K0W68_002219"
     CDS             complement(11221..11478)
                     /gene="trbE"
                     /locus_tag="K0W68_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HBI3073768.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGIL
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(11505..13334)
                     /gene="traN"
                     /locus_tag="K0W68_002220"
     CDS             complement(11505..13334)
                     /gene="traN"
                     /locus_tag="K0W68_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HBI3073769.1"
                     /translation="MVFFLSFSVFADSNSDYRAGSDFAHQIKGQGSSSIQGFKPQESI
                     PGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKDILSPDAP
                     FIQTGRDVVNRADSIVGNTGQQCSAQEINRSEFTNYTCERDTMVEEYCTRTASITGDW
                     KYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFLNSRYTFM
                     NTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDRKVYESLV
                     SGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKTGVMEGKP
                     WNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLHEYATYSC
                     ESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDFGEAVSQLAALAAAGKDVAALNGVDV
                     RAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLTVSVGEFC
                     SRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGITVDELQKI
                     QFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(13645..14025)
                     /locus_tag="K0W68_002221"
     CDS             complement(13645..14025)
                     /locus_tag="K0W68_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073770.1"
                     /translation="MTLPAGYYQIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLAWGWSVKGAFNSKFQLCFRLQPDTPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            complement(14022..14660)
                     /gene="trbC"
                     /locus_tag="K0W68_002222"
     CDS             complement(14022..14660)
                     /gene="trbC"
                     /locus_tag="K0W68_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HBI3073771.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVARALLNNPGDKQK"
     gene            complement(14669..14974)
                     /locus_tag="K0W68_002223"
     CDS             complement(14669..14974)
                     /locus_tag="K0W68_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073772.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(15004..15996)
                     /locus_tag="K0W68_002224"
     CDS             complement(15004..15996)
                     /locus_tag="K0W68_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HBI3073773.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(15993..16625)
                     /gene="traW"
                     /locus_tag="K0W68_002225"
     CDS             complement(15993..16625)
                     /gene="traW"
                     /locus_tag="K0W68_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HBI3073774.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(16622..17008)
                     /gene="trbI"
                     /locus_tag="K0W68_002226"
     CDS             complement(16622..17008)
                     /gene="trbI"
                     /locus_tag="K0W68_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HBI3073775.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(17005..19635)
                     /gene="traC"
                     /locus_tag="K0W68_002227"
     CDS             complement(17005..19635)
                     /gene="traC"
                     /locus_tag="K0W68_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HBI3073776.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(19761..20108)
                     /locus_tag="K0W68_002228"
     CDS             complement(19761..20108)
                     /locus_tag="K0W68_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073777.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(20136..20354)
                     /locus_tag="K0W68_002229"
     CDS             complement(20136..20354)
                     /locus_tag="K0W68_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073778.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPFNSVMETLNGGGQ"
     gene            complement(20453..20926)
                     /locus_tag="K0W68_002230"
     CDS             complement(20453..20926)
                     /locus_tag="K0W68_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073779.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKVC"
     gene            complement(20919..21332)
                     /locus_tag="K0W68_002231"
     CDS             complement(20919..21332)
                     /locus_tag="K0W68_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073780.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(21325..21546)
                     /gene="traR"
                     /locus_tag="K0W68_002232"
     CDS             complement(21325..21546)
                     /gene="traR"
                     /locus_tag="K0W68_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HBI3073781.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(21681..22196)
                     /gene="traV"
                     /locus_tag="K0W68_002233"
     CDS             complement(21681..22196)
                     /gene="traV"
                     /locus_tag="K0W68_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HBI3073782.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(22193..22561)
                     /locus_tag="K0W68_002234"
     CDS             complement(22193..22561)
                     /locus_tag="K0W68_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HBI3073783.1"
                     /translation="MFISLMTEQTEATEIKMNMRNINVITARVVPDKNVSDDFMHAVL
                     SNCTTKIVLPAPKVFSSESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKT
                     NLTAELISAVVGSNKKGEGQ"
     gene            complement(22500..23090)
                     /locus_tag="K0W68_002235"
     CDS             complement(22500..23090)
                     /locus_tag="K0W68_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HBI3073784.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(23080..24507)
                     /locus_tag="K0W68_002236"
     CDS             complement(23080..24507)
                     /locus_tag="K0W68_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HBI3073785.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGTSKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(24507..25211)
                     /gene="traK"
                     /locus_tag="K0W68_002237"
     CDS             complement(24507..25211)
                     /gene="traK"
                     /locus_tag="K0W68_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HBI3073786.1"
                     /translation="MFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVPVTKETLQ
                     APAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMFSQPASQLL
                     AGARMDVYVIRDGEGN"
     gene            complement(25222..>25602)
                     /gene="traE"
                     /locus_tag="K0W68_002238"
     CDS             complement(25222..>25602)
                     /gene="traE"
                     /locus_tag="K0W68_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HBI3073787.1"
                     /translation="NSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRPGAQN
                     QMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTDIKHY
                     ILILKRENGVTWLDNFGETDDEKK"
BASE COUNT         6244 a         6947 c         5951 g         6460 t
ORIGIN      
        1 gagtcacgtc ttctccggag gcaacctccg gttcgaagag gctggccatc tggcgacctt
       61 ctgcttccct tgcagcaagt acggcactca gacggttctc catttccggg ttgatgtcac
      121 gcggaataaa ctccggagcg accttcggtc gtgtctgata tttcagtgag agttttactg
      181 ccggatacgg tccgggcaga gtgacataac aggtcagatc cggcagagac tgaatgtcgg
      241 aataactgac cagcgtctgg cgctccatct ccttaccggt cgataccccg tcacgcacgg
      301 ggtcagcacc gtaggaatac tgcagactgg ctttcaggtg ctctttctca ccaatttcac
      361 cggcagcaaa ctctgcaatc tgatggctcg gggagcggaa aaaggcacgg gtattcagca
      421 catcaaacag cgtggccgcc gctttctcac cgtagatatc ttccagctgg gcataggact
      481 ggataccaaa cacataacag cctccgaact tacgggcttc cggcaggatc tccaccaggt
      541 ccggcagttt gtgtaacgtg ggtaactcat cacagaaaaa ccacacacga cggttacggt
      601 tttctcccat tgccagcaga ccacgaatgg caatggacag ccacatggaa accaccggct
      661 tcagggaggc atgagtgtcg gcatttgacg agataaacag ccagccgttt ttctgatctt
      721 cccggacacc ccgcatccag tcacgtatgg tgaagggttc accgttatgc tcaatcccct
      781 gcaggtaacg gatggctttc acgtagttgg tcagcacagc acggatggaa atcgccgttt
      841 tctcaatttt ctcttccacc aggttggccg ccggtgaatt acgcaggaag gtacgcagtt
      901 tttcgatttt gatggaaagc agtgtgtcca ccagtttgct gtagctgcgg ttggggtcat
      961 tacgcatcag atacgccgct tccgcaaaaa tggtacgtcc tgatccctgc cagaacgggt
     1021 cctctttcgt tcccatcggg atcagcgtgt tcgcgacatt atcaaaatcc ggctgtgtca
     1081 ggcactcctt ccacaaatcc caggcggcac agcgggcatc cagcggattc aggatcttat
     1141 cgatggaggg atcatagtaa cttttaacaa actcacacga acggtcataa atcaccacca
     1201 tgtcgccacg tttacgggca tagttggcaa gacgtcgaat aacctctgac ttacccgtcg
     1261 acactgttcc gtgcagacag aagttctgga tttcggaatc ccggataatc ggcagatcgc
     1321 caatccggat atcggagtcc ttgccgtctt ttttcagcat ccgggcaacg tctttcggat
     1381 tgtctgtcag ctgacgacca cctgtgactt cattttcgct ctgttgttta ccctgacgcc
     1441 ccagaatcca ggagacaaca aagaaggtaa tcaggcaaat aaccagtgcc acaactgtag
     1501 ccagaacgaa tgcggaccat agctgctctc cgcaccagat catatattta tcatgcagta
     1561 cctgagcagc gttcatccgg aaggttttgc cgtaatactg gatctcatag accggctggg
     1621 attttattaa atcccgcatg ccttccagtg acgtacacca ccagtaaata cagccgttga
     1681 taaacgtctg ccagcttatt tttacccata agaccagacc aatgaggatc cagaaaaaaa
     1741 taaacaggca gtaaagcatg atattggcga tctggctgaa catgcggata cgcatggacg
     1801 caatctgacc gccctgggtc atatcctttg cgttaaaact catgatgaaa ctccgggaaa
     1861 atctggaaca gggcagaaaa ggatgatgtg gggttaattc acagaaacag accaggtgta
     1921 ccctgtatgc cggtggtgat gcccgcctgc agtcctgccg gaagagacct tctctgtaac
     1981 aatgtcagtc aggcgcaata cttcagacca gtcactccca tgcggaaact ggatctgaat
     2041 aaaaaggcgt acccgttcac ggtgatccag aactgtgtcc ggttcataaa aacggccact
     2101 gtcaggagaa acacaggacg ttttattgat gagatcatac ttatcggcag gagactgcga
     2161 tgaaatcgcc caggtgatat tctccgcctg gtggccatca atacgactgc tccccggcgt
     2221 ccatgcaatt tcctgcgcac tcgccacgat gatctccctg gaaacttcag ccagctttct
     2281 gggtgtacag aataccggtg ccagcacaca cagggacaac aaattacagc tgaccgcctc
     2341 cggtttaagc aaattgctcc cggatttttc aggaaaagat atttttgtca gtatttcagt
     2401 taattcctta tgcctgagag aattaataaa gtacatgata agcatcatga gaaacaatat
     2461 aacaataagc cctccgataa ccactaaaag gtcagtatca atattattgc tgtacagata
     2521 gtctttagtg gtcactgtat tatcctgtat caccgggggg tattcagttg caggcttctc
     2581 agtaaattga ttattctcac caatatattc agcgatattg tctgatgtca taatgacttt
     2641 ctccggaaga tcccggcttc gccggcaaca cttcacggtc gcacaaagaa tttgcacaac
     2701 cgctcagtgt tttcatattt atttcggaaa cgccgggagg tgacaaaatt tattttgtta
     2761 catcccgggg ttggacaacg agcaacgcga ggcgtcagca aggaggccgg tcagatcagc
     2821 ctcctgacga ggttcagaga atatttgcga ttgatttggc cagctggtct tcgagaacag
     2881 gcttcgcctc ttcaaatttc aggttaacct tgttcgcatt tgagaccaca cgggtctggt
     2941 atttatgctg gttacctgtt tcagtactgg tctgaatttt cgcaccggat gtgccctgac
     3001 gcagggcggc aacattatcc gttgtcaccg ttgccttagt acgctctgca atctgcacat
     3061 ccgtgatcat ggtatagttc acatcttcca ccatcgcatc tgcagccata cccaccagac
     3121 cagcagcaag gcccacaccg agtgtggcac cggcagaatt tgagttatag ccggtaatac
     3181 cggcacctaa cgctgcacca acagctgcgc cttcataacc acggttcagc catccctgag
     3241 actcccgcag atccatctta tcggccttca gcacattcgc ctgaatccag tagtaggctg
     3301 catccggaga agtcaccacc tgatatcctt ttgctttcac agcatcagca attttgccct
     3361 gcagcccact catgtcttta tcagacgtgt ttttgatctg cagaaatacc gtgcgttcgc
     3421 tggcgggttt aagccagatg gtctcactca tctgagtctt cacctcaagg ttacgcttct
     3481 tgattgctgt gctcatcgca ccacaccctg aaagggccag agtggaactg accagtgcaa
     3541 ccatcatcat tttttttgtt ttcatccaga atatcctttt aactaacaga ctcttttttg
     3601 aacagaccta ttactgagac tattgcagag agattataac gatttatatc tatagtataa
     3661 ataaaggaaa taattacaaa cagagttatt gataaaaacc caaataagaa cgtatgagca
     3721 aaggaaacta ttgaaaaaac aatcacaaac gacaagatat aagttttgtt cttatcatac
     3781 ataaaagata taactttcga ctcatctctg aattttttag ggatggcaag atacaacatc
     3841 attccattag taatggcaac aaaaaggata aaacccatta cgccactaaa acatatatca
     3901 aatccagcat ctcggatatc aactttatct gccagcaaac gaagaaatgt cggccataat
     3961 aaaacccata aaaacacata cacccctggt tttatgactt gtaataatgg cggaatatta
     4021 actccctcta cagagagtac atcctttagt ttctgtgtct ccgtttttat atcatagtga
     4081 gtgatcataa tggccccttc agtctatcct ttttatcatc aaccattttt ttcacagcct
     4141 cttctgatgg ctcaggtatt atcttttgac tttctcgctc ttcattatat ttattattct
     4201 gagataatgc ctctgtttta tgatgatttt gcagttcaga atactgccct cttacaatat
     4261 tttcctcgcc acggatactg ttctgagtat ctccgatatt gccacgatat tctgtgacca
     4321 tattatcaac ctgatgtttt acatcattac ggatattact gtcctgcgtt ctttgctcaa
     4381 taatggcctg atgtccctga tggtcagcaa taatatcctg actgccacca cccgaaggta
     4441 cgctctccat tcctttacca atatcgccac gggattcccg ccaggcatta tcaacaccag
     4501 gctgtacctg ttcctgcaca aaaaaccagg ccatcgcccg acgacgttct gcaatctccg
     4561 gcgaactggt atttgtcaga atagcttctg catcctgcgg tgcgcgtttc atcacatact
     4621 gtgcaaactg ttgcgacagg tcttcactca tctgcccgct catgctttca gtacgggagg
     4681 ccatttccgc atattcatgg ctgcgggtca tattcgtcgt gtactggtcg taactctgtt
     4741 tcgctgagtt cagggctgca gacaactggt ccacccggga gtcagcatta ttgtcagtat
     4801 ggctgccgga ctcactgacc ttgcgactgg taaagtaatc gctggcttct ttaaagtcct
     4861 tactggcttt agcgtcaata tcatggcgag catcatgggt agctctgctg ccactacttg
     4921 cagaatgtga atcatcatcc gttcgatcaa gacttccctt aaacccgcct ctgacaccaa
     4981 ccccaccacc aacaccaaga attccaggtt taatccccca ttcagcatga gcatctgcat
     5041 atccgccata cgagaactta gaacctgttg atgccagttc ctgagtcgcc tgctcattac
     5101 tgatattatg cgccttcgca tagctttcca ctgcactgcg catacggctg gccatcatgg
     5161 agtcctgtgc actcatggtg ctgtcagcgc caccggtgac agaatcactg ctcccccggt
     5221 tagaaccaaa ctgactgagc gtgttccagg cactggcaat actgctgctg aaaccatgca
     5281 gggcactttc tgctctgttc gacgcctccc gggccatttc ctgttgcgcc gctgcaatct
     5341 gacgcgttgc attgataccg accggtaaac gggacatcgc tccgcttgca tccatcacca
     5401 tattaccgtc acgggtctgt gtggcagttg cgccactgcc ggtctggtac atcatctgac
     5461 caaacgacgt ggtgctgttg gtgctccagc tgaagccgtt cacgttttcc gtctgcatgt
     5521 tgccgtagga gtaattaccg tcaaccacac tgcctgctgc actggccgtc ggactgatag
     5581 aggaagaggc gaagtggctg tacacgctgg aaaaacctgc gccaaggcct tttaccatca
     5641 tccatgacaa cggggggatc atggctgaaa gatacccggc agtagaggcc aggtcagagt
     5701 atttcagctg tatctgagag agttcagaca gcacgaccgg cgcaccattc atcttcgcat
     5761 agaatgtcat ggcactgttc agaatggcat acaacaacgg ccaggtctgc agccacatca
     5821 gggcaaacac atagcctttc aggacggaca gtgtcagttt gttgaagaca gctgccagta
     5881 ccagaagcgg gaaaatacca atggcaattc ccgtcaggat agtctgtacc attggcaggt
     5941 ttcgcattgt cacatgaccg atagagacat gcgccagacg ttgcttctcc atcgatgacg
     6001 tggtggccag attcaccagg ctggcggtgt caccattacg ggcagcatta ctggtgatac
     6061 cctctttcag ggcatttatt gtgacgttct ggcgcataat ctggctggca gactgactgg
     6121 agccgtagaa ataactgtaa ctgtcactga ccagttgtct gaataacagg tccgggtccg
     6181 gtctgccgcc aaatatctgc tgcacataat aatgccaggt cttgcctccc gtctttgtat
     6241 cgagattcag cctgtctttc agcgtgaccg acgcatcctt acaggttatg aaattattat
     6301 tgttgtcata aacgccccgt agcgggctgg gtcgggaaaa aatcagcgtg taggggtcag
     6361 cggaggccat cagatcttcc agcgtgtatt tatgattcag gtaaatatca cccagcacgc
     6421 agttctggac gtagtcctgg aacagattga tgatttccgg attccgggac aggaaatcgg
     6481 tgcttttcac aatcagattc gccccgaaca gcatgcctgt cttgctgtag gtgacgctgt
     6541 ccggttgggt gaatatcatc tcgtagctga ccaccatcgc atgcccgata cgggtcgtaa
     6601 gagaaagtgg catcgccaga ccgaccggaa cattatccac ccgatggact ttgaccaggt
     6661 cactgttatc aataatctgc accgatgtgc ggacattaac cagcaggctg ataagcacaa
     6721 acacggccac ccagcccagc aaatccatca cgttatgccg ctgtacccac ataaaagcca
     6781 cagcaacaac agacagggtg agcgcgattt tttcaattga atcccaggtc cgggtactca
     6841 taaatgcagc aatggcgttc aggttattcc gcaaccactc accaccggca atcacataaa
     6901 cttcattcac agtgaattcc ctccgaagtg atagttgttc tggtaacggc tgagcatacg
     6961 ggcggaaagc tgctgacgca tgtagttcat ctgacgttcg acaaccagca gcgcatcctg
     7021 ctggacctgt acctgactct ggaaaatggc aatctgttgc tgtgcctgat tcagattctc
     7081 cagaacattg tccatcacag cctgcggata gttcccggtg gcaatcatgg cccgggcctg
     7141 ctgaagcagt tcctgaatgt actggagcat gatgtcgtag ccgatatagt ccgtcagctg
     7201 gtaaacgacg ctgttggaca caccgagcat ctgcgggtca atcagatatt taaaaacggg
     7261 aatggtggtg ctggaaataa acccctgttc ccgggcgtca agcggagtgt cactcactgc
     7321 tttattctga atactggtga gcagttttgt aatctgtgac ttcagggctt tatcccgggc
     7381 gatggtcaca tttgcatccg ccaccacctt cagacacttg tccgaatcgt tgcagtggta
     7441 aatttttgcc gttcccccct ccataatcgc ccggaggata tcgcggtcag tggtacgcgc
     7501 cggcaggggg gtaatctcgc tgtcttcccc aaaaatcagc gtaccggtga gcgtcatgat
     7561 aaattctttc aactctttgt tgccgtcaaa cattctgttt tttttcagcg cctcccatat
     7621 gatattctgg tttttcagca cccgctcctt gtccttgtcg ctggctttgt cacgaacaga
     7681 tgaggactgc cctccgaccg tgcacccctg acgagatgcg gcccagtcgg cgaagatatt
     7741 gctttcactg ccaatgtcct gacaaacttt ctgctgagat tcctgcgtct gagggaacag
     7801 cccccccaca agcccctgtg ccacctgaca ggaactgagg ttcatactgt taatgtcact
     7861 tgccattttc tgcaggaagt cttttgcggt ttttatttcc ggcaccgttg tctgcagggc
     7921 aaggtcaaaa aagtaaccgg cagcattgct cataatctgc ttaacaaaac gctgcaactg
     7981 atcgccatta ataaaactga acgagccgag ataggcgtcg atgccgccac atccggcatt
     8041 gatatccggc agcgtcatgg aaatcagctg gatatttttg acctgggtgc gggcatacag
     8101 ggagccacca taggcatacc cggcggcctg cccctgccag acgcccggct gtgtggtatt
     8161 ggaggcgaag cccagcttgt taaagaactg attcatatcg ctgttcacat ccgctgacgc
     8221 tgcgggtgtg accgtcagta aagcggcaca aagaaccaga agaggtttaa tgcgaagcat
     8281 tattgctgcc ctcccttaat gtgttctccc ggcgcatacg gcgtttcagc aggaatatcc
     8341 ggacaccccc aaatattatt tcgcacaacg gcgacagaga agccgcagca agaaaaccag
     8401 ctgcaagcgc atataacata atcggaatga catacatcag ggtgttccac atataacgaa
     8461 aattatttaa ttccgggtca gctttccacg aatcaatacc ggcactgaaa aaatcatgca
     8521 taacgttcct tacaatcaat ccggcagcaa agaaaaccac aaaaagccag aagtattttt
     8581 ttgcattatc acaaccacgt tcaatctccc tttcaatttg tttgattccc ggttctgtat
     8641 catttatttt ttctttcatc ataagttccc tctgatattt ctgatgattc atgcagacag
     8701 gtcatgcggc tgtaatatat ttcagacacc gaaatccatc ccacaattgc ccccacaaaa
     8761 aacacaacag aaacagaaaa ggtaagacac agcgaaacga caatttccac agggctggcg
     8821 ggataaaaaa gcccctgacg gataaagggg atattcaggc tgacgattat tcccgtcgcg
     8881 atgccggata ttccggcaat gagtaacacc acctgcttat ttcgcacctt tttcctctcc
     8941 gtatatctgc agaacggtat ccatccgcgc cataaaactg gcggcatccg ttgccccctg
     9001 caaaagcggt aatgcctcaa gtgtgttgac gttgaccagg aaggtggtcg gtgtggccac
     9061 cgggatattc gggaagaagg tctgcatcac gtccggtggc accggtaatg cttccggaaa
     9121 ggcagtatcc ccctgaccat ccagggtgta gggaaaaacg gaaaagccgt actgctgtgc
     9181 cagctgcttc agtaccgggt caaactggtg acagtaaggg caatgcccct gcataaacag
     9241 caccaccttc cagtcggcca ggttaacctg cctgccgttg ctgaggcgga accagcgggg
     9301 agccggcttt tctgttctgg ctgatgtgtc tgctgccggt tgtgcaggct gcacggccat
     9361 tccctgcgga ttccagagtc gttcaatttc atcccgggtg gaggcctgca cacctgtggc
     9421 catcatcaac aaaatgaccg gcagtaattt agtgagagac atgtccgccc tcctgctgcc
     9481 cttccatcat ggcctgttgc ctggcttcca gtgcctcacg ctcttccagg ctgacacgtg
     9541 caatccatgc atccagcacc tggtatgcgc cgaacagcac aaggatggcc gcaatcaact
     9601 ccgccagaaa aaaagccatc cagggtttgc tgtaaacaag acgggcgacg atatgaaacc
     9661 agacacccag ggaaaatacc cacattcccg taatatcaag ccggggtaaa gagaatctgc
     9721 gtttacttat catgttaaac tccggttatt atttacatga gagtattcat gtctgtaata
     9781 ttcgcgtttg tgtgatatta atcagaggta caaatgaaaa agttatcttt taaagaaaaa
     9841 ctggaaatag tgagaaacat cataagagag tcattatcag gtaatgcggc ttttgcttta
     9901 ctggtgtatt cattattgca ctccctcccc attaaaacct ttgctgattt atttgtgacg
     9961 atactgttat ttatagcgat tatcgctgtt gtgatatggc tatttctaat catctatatt
    10021 tatttttgtg agttattcag aagtcactgg atagcagtct ggtttatcct ctggtctgtg
    10081 gtaatcaacc tcactattgg atttggcatt tatgacaaat tagcaccagt catttcacta
    10141 atgaaataat aaaaaaccac atcactccag accatatggc tgacaacata atacaaacaa
    10201 acatccttct gaaactgtca aacttcgtga taaccggtat ggtactgact gccattgaaa
    10261 caaccggtac ggcacactga ttcataaccg caccacaaag aaacaagaaa ttatctggca
    10321 gcatacatac acttatgaca tttactacaa agaagacaaa cgagctcagc agataaactc
    10381 ctttattatt tggtcctgta aagaaataac caacaacccc cacgataaac aggctggcac
    10441 ctgtaaaaaa acacaaatcc cctgtatgca tatataacct ttggttaaaa attcggttta
    10501 aaatcttcag aaacgttcag gaactgtttt gccaggtcgt cctgtgaaat aaagccgtat
    10561 gataacgggc gaacactgcc ctgtttcggg tcaaccagca tcatggccgg gaaatatttc
    10621 acaccgaggc gctgcgcctg cccctggtca gtccgggaat ccggcaacag cggattaatc
    10681 acgccatcca cggaaacggg aataacactc agaccatacg tatcccggaa gccattaatg
    10741 acctgcgcca gttgcccgtc gatggggtcc tgcccccggt aaaaaaacat gatgccgtag
    10801 tgttcagcca gtttcgcaat ggcctgtcgc tgctgcgcct ggtctgccgc cagctggttc
    10861 cgtaccgtgc cgttgtaatg gctgtactgc aggttatagt ccagttcggg atgtgccagc
    10921 atggcctttt tagcactcat ggtgaaaagc ccggcctgct gagtccagta attctgcagc
    10981 cggaaatatt taacaaaatt atccacgccg ggatacagaa tggcttcgta cagcgcccgc
    11041 tttgttgccg tctgcagcgc ggccagtttc tgcataatat ccggctcctg acggggggct
    11101 gcaggtacag gtttattttc tttttccttc ggatttattt tctcgttata ccactgccag
    11161 cctgcatctt tccctgacgc cggaaaaata aagcagcaga gtaacagtgg cagtaatgct
    11221 ttattcatct tcattccttt catcattttt tctttcatca tctttctgca ggccagtcag
    11281 ggcagaaaaa caaaaataca gaataaacag gagcacaccg gagtaaagga taacgaaagc
    11341 agcagcaagt atcccgtctg ccgtagtttc ttttaccgta tcccacgacc agataattac
    11401 cggagaaatg acaatcgctg taatcgaaag ccggataaga aaatcaacaa gacgattaga
    11461 agtgaatata actttcatca tttcccccgg ttattttcca taccttactg tcctgcctgt
    11521 ttcagctggt cagcaatctg ctctttcact ttctgcgtca gaacgccact gtccgggatt
    11581 ttctggttat tcatcaggtc ttcgtagaag ttagtgaaat ccagtctgtc gaactgaatt
    11641 ttctgcagct catcaaccgt aatcccccga cagtcaggat gcttcgcact gccaaacccg
    11701 atacgcaact gcccgttacg cccctgctgc tggactatct gcgccagctt cgaatcaaac
    11761 tggcagtagc tgcgcttttt ctgcagacag acacccagca ctttccggga gcagaactcg
    11821 cccacactca ccgtcagttt gttgtccttt gcctttccca gcgccttttc ttcactgttg
    11881 cacttcgcca gccccacatc ctgaccccag ccgccatcct tacagcagtt actgaagccg
    11941 gctgcggcct tacggcagaa tttcgcctca cccgtgaaag ccctgacatc cacaccattc
    12001 agggcggcaa catccttacc tgcggcggca agcgcagcca gctgtgatac tgcttcacca
    12061 aaatcactgt tttttccgct ctgtgcctta tcgcattcac cgtcaaggca gaagacatca
    12121 ccaccacaga ccatcacttt tcctgatgtt ctggactcgc aggagtacgt ggcgtactca
    12181 tgcagacagg ttccctcatc ggaataaaac gcgcactgac gtgaggccag cgtacaggcc
    12241 ggattatcga catatttctg acaggtgccg ttatctgcgg actgagtgac atatttatcc
    12301 cggtatgccc agcatgcctg tgtgatgttc cagggtttac cttccatcac tccggttttc
    12361 gtaccacccg gctctgaaca ctcagtgcct gtcagcacgc catccgcttt attaaacgga
    12421 caactctcca cccactccac ccggggaacc cattctttat cacggacctt catcctgagt
    12481 ttcagcgtaa aggtggaagc accactgaca agcgattcat agactttcct gtcaccattc
    12541 ccgtgcggga ggcaattacc gttagcagta cagccactac cggtcagaac ctgcccctgt
    12601 gtcacctgca aacctgtcgc cccggacagg gggtaagtat ccgactgccc gtttgccagg
    12661 ccgacattaa aggttgtatt catgaaggta taacgggagt tcaggaagaa aaaagcggcg
    12721 tatacagaca atgatgcact ttccactgta ccggttaccg gagccgtgac actgaacacc
    12781 agtttcagcc cgttcatact gaaccggaac tgtgagtgcg gtatcgtcac ttcacgatat
    12841 tcatccgtgt atttccagtc cccggtgata ctggcagtcc gtgtgcagta ctcctccacc
    12901 atcgtgtccc gctcacaggt atagtttgtg aattcactgc ggtttatctc ctgcgcactg
    12961 cactgctgtc cggtatttcc cacaatgctg tcagccctgt tcaccacatc cctgccggtc
    13021 tgaatgaacg gtgcatccgg tgacagaatg tctttcggct tgttcatgaa ggactctgtg
    13081 atggtttttc cggtttcacc ggtcgcccat tccgtggtgc cgtcattttt caggccgcca
    13141 tccccgccgg cagtcacgcc accgtaatat tttgtctcgt ccggattcgc gttatagccg
    13201 gggatactct cctgtggctt aaagccctga atactgctgc ttccctgccc tttgatctgg
    13261 tgagcaaaat cagaaccggc ccggtaatca ctgttgctgt cagcaaacac agaaaagctc
    13321 agaaaaaaaa caattgcaca aagattgcac ggctttacgg aataattaaa attcagagat
    13381 ctgaaaaagg tatttatctt ttctttcata acctgaacca tactggagaa tgaagtgatg
    13441 tcatctcaac ttcagaactg tgttgattgt ggtaaagcat taagtcgaac agccacaacg
    13501 tgcgggaatt gcagctcaac ggaccctttt ggaaaaaaac ggcgcagcga tcgcttttac
    13561 cagtggtttg tagggctgtt tattacggct attattattg ctggcggatt atggtacatg
    13621 gggcttcttg acgtaccggg attactactc agataacgac ttcagtaacg atatcagtgt
    13681 tctgaaatcg gcacataacg agtgacgaca ataccggtta taccagtaat gaggggtgtc
    13741 gggctgaagc ctgaaacaga gctgaaactt actgttaaaa gcccccttca ccgaccatcc
    13801 ccatgccaga cgtggtattg ctgattcagc atcctgccgc agtcgctgct caagaagggc
    13861 agccattttt accggacagg caaaggcaat ataaggaggc agctttgtaa cagggaaacc
    13921 atgtccctga caggaagcat atgtgcggaa gccgtgaatg ttcatcgcgg ccaccagggc
    13981 acggatctcc ggatcaatct gataataccc ggcaggtaaa gtcatttttg tttatctccc
    14041 gggttattca gcaacgcccg agccacctgc cggcagtccc ccgtggcggc cactttctcc
    14101 agcgcctggc cgacccgcag gttacctcgg ataatgtcgt atccctgact gcagaacacc
    14161 accagtgccg gtacactgcg aatgccgtac tgtgaaaaca gcgtcgggtc gatctgaaca
    14221 ccatcagtcg cgccgtcttt caccagggac agcacggctt cagcggtggt cttcaggtca
    14281 ttattcacca tgccccgcag tgtggcgggt ataccgtagt gccgggtttc acccagcatc
    14341 cgtttcagcc cctcttcggg aatggaaaac gacacaaaat acagggcacc ctgccggggt
    14401 ttcccgtcct gcgaagcctg ctgtttacga accagctcat ccagaaaatg gttatctgaa
    14461 cgctgaaggg ggttttccag cacctgtttc tccgcccagg ctttcagctg atggtcaggt
    14521 ttctctcgca gttgtctgct taaattttcc tgctgcttca ggaactggcg gttttcaggt
    14581 gtgttcacat tttctgaagc atgaacagca agatgcatcc ctgctgtcag agcaaaaagt
    14641 gcaaatattt tccggttcat gatatttctc actttctgct atttacaggc atgaactcat
    14701 cagaaaaaag ataattgaga aaaacagcca taaacgcaca tgtaaatgtc accttcagat
    14761 ataatctgac aagtgtcact tgttctgata tatctgcctt cttccattca tgaataattt
    14821 cagcgagaaa actgtcaggt gcaaaataaa ataatataac gggcaccatt acaagaccaa
    14881 aaggaaataa ttttaatgtt ctgcttatca gcctgagcag cacagcactt attttcctga
    14941 ggtgctgaat atatttactg tccattgttg ccattttcat ttcctcttat tcattaaaat
    15001 gatttacagg aatacacagt tacgtttacg ccacatcagg tagccgaagt ttttctttgt
    15061 gttgggcgga ttttttcccg tctcccagcg catcacactg cgcccgaacg gatggcactg
    15121 cccgctgtcc ggatacatat tcaccatctg ataacgccag cgttctttgg gcaggattgg
    15181 ggacggatat tcattacaga cggcgttatt tttcccgatg gtttccataa tcatgccctg
    15241 acggtgtagt ttgaacgcca tgcgttcact gaccagcagg gaggactgca acggactgga
    15301 ctcattactc acccagccat tgaacgggta catacttccc tgcgaaccgg cacaccagaa
    15361 cagaacatcg agaggcatat taaaggcgct ggcaatggca tctgctgcac aggccccctg
    15421 tgctatcgga ttggcaaaga tgacagcttc cggattgaga atggtggtca ggctgctgtc
    15481 cgtccaggtg gggtcaattt cagaaagata agcgatatcc aggtcaccac cttccagaca
    15541 gcccagcgat gtgatgatgt tcagccagta cgtcagcggg tatttgtacc agtgaacgtg
    15601 atagaacgcc ccgttcacct gcttctcatc ctttttcgcc gttccctgtg ccgttttacc
    15661 aaaagccggc aggctgaagc ccaggttaac catgcatccc ggtgaccggg tgacgtccgt
    15721 cagcgccatc ggctcccagt aaccaatggc cagcccgata cgcctgaaca gcggcggcgg
    15781 tgccggacaa atctgaatgg gcatcgacgg gttcgccgtg tcggggacct taccctgact
    15841 gactttgaca ctgcccagcg agagcgggaa aatacagctc cagcagatat ccgtgatcgg
    15901 gtttacaaaa cgcccctcac aggcagaatc cgcagacgct gcagggacat gaccggcgag
    15961 aaggaataac atcagcagcc acaggcttcg cttcattttc tgccctcctc tgccggaata
    16021 aattccacct tcaggaagcg atcgccggga acggcgctca cccgtgccgg gacctgatca
    16081 atgccgaggc gctggcagag cacaccgttc tggtcaaagt aaacacggct gtccagagac
    16141 ttctgcatct ccgggatact gccctgcacg aggataattt tgctctccag tgtgggcggc
    16201 gtctggcgtt tcatccaggc cacctgcgcc ggatcatcgc cattgatgaa atacagcgtc
    16261 tggttaaaag gcacatactg cagcggattc atcacttcac cctgacgggc aaacacccgc
    16321 ccttcgttat cccggatatc cgcagccagt ctgacggacg ggtcaaacaa ccggctgccg
    16381 tatttctccg tgcgtccgat accgggcacg gcagggggcc gcagactgtt gcggatcacc
    16441 cgctccttaa aggcatccat tttccggccc atctcaccgg actgctccag tgccgtcagg
    16501 cgctgcatga tcaccgtcag catgtccggc tcctttaccg gccacagatc gccccaggtg
    16561 ccaagatcgg cggcggccac actctgcccc catatcagca gggcaatcag cccccggcat
    16621 ctcatggttc cgctctcatt cgccgggcaa tatcctgctg gatttcccgg gtgatatccg
    16681 gagccccctg taccacggca ggcgacacca gaatgaccgc gtggtgtttc tgctgccacg
    16741 cctgcaggct ggcttccagt gccgtgttaa aacgcgctga aagggctttt gactgtgctt
    16801 ctgacagttg tttctgactg gcgctgtcaa aaaatgcatc cacagtctgt ttcatgttga
    16861 aggcggcagt caccggtgcg ttcagtctga caatgccgta actgacggct gcgttcagta
    16921 acaccattgc caggcatccc ggcaccgtcc accaccagtg gctgcgtcgg ggggctgtaa
    16981 catcagtcgt tttttgcgtt gtggtcatgc aacactcctg tatttctcat gttcttccag
    17041 ccaggcttcc agcgaagcca tctccggccc tgacttcttc cacgccagct gccacaccgc
    17101 ctcatgaata ctcaggccct ccctgcgttt ctgctgcaca aactcaaaat ccgggccgtc
    17161 agagctgtac atggcgcggc ttaacgggtc cacaaacaga cggtgccagg aggaatggtt
    17221 ttccacctgc agcaggaagg aactgaacca ctggtctttg gccgcaccaa acttgccaat
    17281 catgtcgcgc tgcagtggct ggaactgatc cgggaataac tggttatatt tcgcgaactc
    17341 cttcgcactc tgtttgagga taattttgta ggaggagtta ccccatgccg cacgggccgc
    17401 actggaggcc ttgtcagagt caaagtcgac gatgttctgt gtgatggtga tataggcacc
    17461 ggtatggcgg cgacaggtgc ggtaaccttt ttgaataaac tcaccgacct tgcgattttt
    17521 gaagtccagc agacgccagc cttcatcaat gacgttcagt ttcttgagag tacgcggcgt
    17581 gcggtacatc ctgttctcga tgtagataat cagggagaac atcaccgcaa ccagcagtga
    17641 cggacggtct tccagtccgc ccagctccag caccaccatt ttcgcatcat cccgcaggga
    17701 cggctcatcg gagttaaaat actggccgta agtgccgttg gcagtgtact ggtcaagcag
    17761 cacaatcatt tcgtccagac ggctgcggat ggtcggtgac ccggcatact gctcgctgtc
    17821 actggcgttt ttcaggaaat ccaccacgtc atcaatacgt gccctgttct ctttggccag
    17881 ccaggaggcc ctgaccgcct gcagcagcag accttcatgc acttcatcca ggttaccgtt
    17941 ggggctggcc atcactgaca actggtcacg gacacgctcc gctgactggt caatatcggt
    18001 gatgttcgca aacgggttaa aacgcagggt ttcaccgtcc agatatacgc cccccatgtt
    18061 ctcacacagg gacttgtagc catcccccat gtcgaacacc acggcaaagc ccccggagtc
    18121 cagcacgctg cggataagtg gctgtatcag tccggtttta ccggccccgg aggtgccaca
    18181 gaccgccatg ttgtagttgg tgttattcat ccccttaaag aagatatcga taaacgccag
    18241 ctggttacgg taggtgggcg ccagcagacc tgccggtgtc agggggttat ccgccactaa
    18301 cggcatcagg ttggccacat taaagctctc tgcgcgctga accacaccgg cctctttcag
    18361 ctgtttaaac agccctttcc cggccataaa gggcaggcag gtcaggaaat tgcgcatgtg
    18421 gttaaagcgc ggtgaaatca gctcaaaacc gtttttacgg aagctgttca ggatgtcctg
    18481 ctccacttcc agtgccgttt cattattgtc cttgcagaag gctgtgatgt tgaggaagta
    18541 ggacacgaca gaagactggc cggagcccag ccgctggcgc agttcccccc actccttcgc
    18601 ctctttctcc acggacggaa accatttggc atatgaggtc ttcgacttct tctccaggtc
    18661 catgtacttc aggttggctt cgctgtgggt tttcacctgg tcttccacca ccagagtcag
    18721 cgtcaggatg aacgggcagg aaatggacat ttccgggttc agcagattgc tgtagttgtc
    18781 ggccatgttc cacaggaagg cgatttccgg gttacgggcc agatggaaat tcaggatgcg
    18841 ggccgtgctg ttcctgccgt tctcacgcag gcccagcgtc aggtaatcag cccggacatt
    18901 caggtcaaaa ctgtcctcca cacactgata attcagatca gaatacgggt ccagctgacg
    18961 tcttttcggg tacagagagt ccggattatg gttaatcatc tccccgacaa tctcgatgaa
    19021 agcctgtgca tccactgtct gtgtggtgat ttttgcgccg tgaaaagagg cacggatgat
    19081 tttcaccaga ttttccattt ccagaatgtc agcccggctt tttttcttcg agggagaaca
    19141 gtacgagata aacacccggt aatgacgcag ggtcaggggc agattcatcc cctccggcag
    19201 cgggaactgt gtcgctgccg ctttcatata ataggcacgg gtaatggcgt taaaccgttc
    19261 agcctgttca cctgaccagg agaactcacg cagcccgtat tcaatcctgt caccaaccag
    19321 ctgactggac atcagatgga tacacaacgg aataccgcgc ggcagtttgg tgcgcagcat
    19381 gtgatcgagt gcctccacaa tggacttatt cgccccattg atgggaatgg cttccagcat
    19441 aaagcccatc gtggtgtcat tcatgaacag accggattcc tggttgtaat cacggtacgg
    19501 cagcagacgg ctgaactgcg ggaagctcat ttcgcccaga acttcattgg cctttgcgga
    19561 ttcgtcaggc agtttcagtg ctgtgacgag ggagttaacc gcctgagtga cggcctcaag
    19621 tgggttatta ctcatatgag tattccttag tgcgtttaaa aaaggaaaat atcagtctgt
    19681 ggccgtggat atatcacgca gtgccattcc tgaaaccaca gcacaaataa gagatggagg
    19741 cttttaacag gaaaccagga ttaacgttcc tttactgaat gagcatcatt taatgcctga
    19801 atttgttttt cgcgaatctt attgcgctca ttttctctta cccgtttcac ctctgccatt
    19861 tcgtttcttt ttctgcattc tgatgcgatt ttatttgcct taccaagtgt taaaggttca
    19921 gactgataat taagtgtctc ctgaaatgct tctgctgcac agggagtttc ctgtaaaatc
    19981 tctgtcagtt gttctggtgt tgcaggttgt tgcgcaaggt aaacgatgta aaaaggaata
    20041 catacaatca tagccataac gccaacaaat ataaaagcca gaacagattt taaatatatc
    20101 gacttcattt cattttcctt cgaagcagta agcactcatt gcccaccacc atttagtgtt
    20161 tccatcacgc tgttgaaagg ttcatttgtt ttaacttcaa tacactcccc tgtatttcca
    20221 aagcccctgc cgccatgtgc ttcgattgtc cgaagaacct gcacgcatac gtccgtattt
    20281 ggcatgggtg taatattcca gcttccgcca ccgttaatat tttttatcag cacaagaaaa
    20341 ataaaggtat gcattcttta ttatctccgc ttgtatatta atcactgaat atcccttcag
    20401 ttaacttatg aggtcataaa acactgcaga ttttcattca gatttactct cttcagcaaa
    20461 ctttactctc ttcagcaaac ttccggatgg tatcctgttc acgctgacgg gtggcagact
    20521 cctgcattgc atccactttc tcctgccaga gacgccggat atccttttca tcccgaccag
    20581 tcagtttttt accggacagc agacggttaa gcagttcctg tctcgcatcc ggtacgtctt
    20641 cagtcagacg cataagctga agcaggctct gacggctgag aaccatgagt gattccggca
    20701 ggagggtgaa gaagaaaata aaaaagcata ccgcaccaca gcaggcaagg accgcaaaca
    20761 ggattttcag atatatacca tcaacgccga acgcagcaat aatacaggga atccatgcaa
    20821 tacacgcaca atacgccagg atgttatcta ttcggttatg tctgcgatat atctgttgat
    20881 gttcatccag aatagaatct attttttcag cttctgattt atgcatagtg tttcctctgc
    20941 gtttcctgcc agagacgccg gatatcctgt tcatcccgac cagtcagttt tttaccggac
    21001 aacaaacggt taagcagttc ctgtctcaca tctggtacgt cttcagtcag acgcataagc
    21061 cggagtaatt cctgacgact gagtaccatg cgtgattcct ccggtacgct gtaataatta
    21121 aaaatacgac tgaatacccc acaacagcag gcaacggaca ggcacaggga tttcagatac
    21181 aggccagaaa cacagaatgt ggcaacacca caaagaatag ctataactaa aaatccctta
    21241 cccagtgcca tacacaaaga accctgtttt ctggatatcc gctcatgctc atccagaata
    21301 aaatctattt tttcattttc tgatttatgc atagtgtttc ctctgccttt cctgatacgc
    21361 ctgacattca acgcacaacg tcacaccggg aaatattttc cgccgggctt ccggaatagg
    21421 atttccgcat gcttcacaga gataaacagg aataccatga acatttattt cctggcgtat
    21481 ccggtttatt cctgtcatgg tcagttgttc tgtcactgaa tatgcttcat cggcttcatc
    21541 actcacggaa tattcctccc gacatgttct gacacgctac cgcccatccc ttttcacgca
    21601 cagggcactc gttttacagg cggattttga atacagaaag cccgccggca ttgccggctg
    21661 atatacaact tcagggccag ttaattaata cgtggttttc cccacgcaga cggtttaata
    21721 acaaaaaaca cgctggatgg ctgatgataa acatcctggt tatcaatata aggcgctatc
    21781 cataatgctg ccgtctgctc acccgttctt agcggacgag gaggtgttac cggcgtaact
    21841 gaactgaccg gaacaaccgt tttcacttcc cgggcagcgg taaacagcgg gcgtggtgcc
    21901 agcagtttct gctccggatg tgctgtcacc gcaggtctgc tgccggacgg agtggtcgcg
    21961 gtgacggtct gcaccggcat tgtccggaag ttcccttcag ccaggcgcgg cagtgatgcc
    22021 gcaaccggct ttgcttctga ggactgctcc agttttttgg ccttctcatt ggcctgctcc
    22081 atcgtcatac aggtatcgga tgtggtggcg ttacactcaa attccgtatt tgttcctgcg
    22141 cagccggaaa gtaataaggt tcccagcaga ggaataaata aagaaatctg tttcattgcc
    22201 cttctccttt tttattactc cccacaacag cagaaattaa ctctgctgtc agatttgttt
    22261 tccctgcaca gggatgtccg gagaccactg tatttcccgg agaagaaaat aagctcatcg
    22321 acattccctt cttcatcaca ccgacagcag ccatattaaa tttgtgcggc aatgattcag
    22381 aactgaacac cttcggagca ggaagaacta tttttgtggt gcagttactg agaacagcat
    22441 gcataaaatc atctgatacg tttttatccg gcacaacgcg ggctgtaata acattaatat
    22501 tacgcatgtt catttttatc tccgttgctt ctgtttgttc ggtcatcagg gaaataaaca
    22561 tggcatttcc tgatttttcc cggcagggaa gcgtcttccg tataaacgtc cttcagatat
    22621 tcatctgcgg ttaccacccg gatacatttc atttttccgg gcagcaccgg atatttcccg
    22681 gtgatcgctg ccagcgagac atacatcagt gccagtgttg cccataatgc ggtgaccagt
    22741 tgcatcagtg accggtcagt ctccctgaca taatctgcag catcagccgc gcggtaatgg
    22801 cagatatcct gtatttctga tctgcctgaa gaatcagagg aagaaaacca gcattcactg
    22861 accgggaact gtccggcagg tgctgtttgt cttacgaagt taatctcctt aaccagtaat
    22921 ttccccggcg tggtgttgtc cttccataaa acaaacagag ccatcagggc cattgtggcc
    22981 agtggcagaa tgacggtgta cttcagacac cagaacagtc ctcgcagaac ccgggcgaca
    23041 acataccggg cggcatggca cgcctgacgg gacgacatat tatttgccat cgttgcctcc
    23101 tccctggctg ccggacataa actgctgcgg attcaggttg cccagctgct tcagcatctg
    23161 gtcagtatta aagccgttaa gatgactttc agctgatggc ggaacaggaa ccggactttc
    23221 cggattatct tcttccgctc tgttctgcgt gcgctccagc gccatctctt ccacggtttt
    23281 cagctggaag ccgtcctgga acaccacggt cacttcgttg cccgcaccaa tcggtatcac
    23341 cgggtgatac tgttcggcac gtttgatgta gtagtcactg agcgtctgtg cggctttcga
    23401 tgtgccgcca ccgataccca ttttcaggac atcaccagcc ccgtaagcgg ctgtggcacc
    23461 cagcccgaca gccggctggg aggcgcgctc cattccctga ccgataccgt caacaaatcc
    23521 cgcgccccat gcccagccga ggattttgcc gttacgcatc accacttcgc ccttgatacc
    23581 gtttttaccc cggaagctga catgcccctt aatcggcata tcaatagttt tgccgtcctt
    23641 caggcaactg atattgcggg tgcgtacaat ggcacgctca ctggacacat ccccccaggc
    23701 ttccagcccc acaaaacatc ccgttgcgtc atacgtcttg ctgttcggca tttccaccag
    23761 gccggtgata cgcaactgca tcggcaccgt ggattcatta ccggtgactg aggcattggc
    23821 atccgcccct tcaatcagca tggctttcgc aaaacttcct gacggaatgt acggcagcga
    23881 tggtccctgt tttccctcat tacgggtaaa caccttacgc tgtatccggt taggcaccgg
    23941 cacagactgg tacgtcacct gtggtggtgg cgtgacacca ttcccgggat aaaacgccgt
    24001 cggcggtgga acagcaacgc tgccctgcgg cgggaaggat acgggggtgt ttcctggctg
    24061 tggttcgcct tccggcccgg gcggtgaggc aggcatctga ggtacaggct caccggtcgc
    24121 cgtgacggga ttagcaccca gggcttttac ctgctctgcc agggcggcat tgtcctgtcc
    24181 cagcttttca atacgacgct ggtcatcacc gcgctgtttg ttcaggacat ccagttcacg
    24241 acggatttcc tcatactgct tctgcatctg cgctgccgtc acctgcatct ctgtggtggc
    24301 atgctgacgc accttgtcat caaaggtcgt atccaccaca cctgtcatat ccggcacagg
    24361 ctcctgttca gccaccgcct caccgttacc ggacatgtcc acatcagaca gatacagtac
    24421 cccaccaatc gcggaggctg caccgacaac cacaatcccc agccacaggt actgcttgcg
    24481 tttcacaatg gtattgatac tggccatcag ttgccctccc cgtcacggat gacatacaca
    24541 tccatgcgcg cacctgccag taactggctg gcaggctggc tgaacatcac ggcacgggtt
    24601 cccggctgcc agaagtcact ttcccggata ttcagggcag acagcgtttt attttccacg
    24661 gcaaagcgga ccatcttcag gtgattcccc gtccatacgg catctgccac tgaagacagc
    24721 cccgccgggg cctgcagggt ttcctttgtc acaggaacct gataccagcc tgcaggtaat
    24781 tttccgccac ggacagcctg gctgatggtc acaagcaggg attcgtaagg cgtggacgtt
    24841 tcccacgctc cggcttcttc tccggttccg cgcaggtcac tgaccagctg aatggtacgc
    24901 cccgcgcctt cacggggaac agcctgaatg gaaaggttca gaccacgctc tgtttccaga
    24961 atgaacgtaa agggcttttt gttgacggtg gccaccacca caccgccgga ggcggtctgc
    25021 tcattattgg tcagggcacc atccagactg ttcacggcga taatccggtc accgggaacg
    25081 gtaaacagat tcgggctggt attgctgatg gcaatactgg cctgaccacc gttcaccatt
    25141 ggcaccacag tgggggccag cgtaccgttt gcggccatca cgctgcagga aaaaaacagg
    25201 ccgccgaata ttattgccgt attatttttt ctcatcgtct gtttccccga aattatccag
    25261 ccaggtcacc ccgttttccc gcttcagaat aaggatgtaa tgtttgatat cagtaaaagg
    25321 ttcggagtta ccaatccatg ttttccgaat tccacggatt tccacacgac catactgagg
    25381 ccagacacgg acactggtct ggaaaaaagc agaattcacg ttatcttttt taatgagttt
    25441 cgcttcttca gccagaataa ctttcatctg gttctgtgca cccgggcgga tatattgcag
    25501 aagcgcctga tgtgaagcat cgacgatttc cggtgagaca ttcagacgga gggcaataaa
    25561 tgacagcgcc atctgctgca aataagaggc atcggcactg tt
//
LOCUS       DAETQN010000055        25503 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.055, whole genome shotgun sequence.
ACCESSION   DAETQN010000055 DAETQN010000000
VERSION     DAETQN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.055"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            15..485
                     /locus_tag="K0W68_002239"
     CDS             15..485
                     /locus_tag="K0W68_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBI3073788.1"
                     /translation="MVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTH
                     KWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPL
                     LVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            487..906
                     /gene="gspB"
                     /locus_tag="K0W68_002240"
     CDS             487..906
                     /gene="gspB"
                     /locus_tag="K0W68_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HBI3073789.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1144..1455
                     /gene="rpsJ"
                     /locus_tag="K0W68_002241"
     CDS             1144..1455
                     /gene="rpsJ"
                     /locus_tag="K0W68_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBI3073790.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1488..2117
                     /gene="rplC"
                     /locus_tag="K0W68_002242"
     CDS             1488..2117
                     /gene="rplC"
                     /locus_tag="K0W68_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBI3073791.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2128..2733
                     /gene="rplD"
                     /locus_tag="K0W68_002243"
     CDS             2128..2733
                     /gene="rplD"
                     /locus_tag="K0W68_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBI3073792.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2730..3032
                     /gene="rplW"
                     /locus_tag="K0W68_002244"
     CDS             2730..3032
                     /gene="rplW"
                     /locus_tag="K0W68_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBI3073793.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3050..3871
                     /gene="rplB"
                     /locus_tag="K0W68_002245"
     CDS             3050..3871
                     /gene="rplB"
                     /locus_tag="K0W68_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBI3073794.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3888..4166
                     /gene="rpsS"
                     /locus_tag="K0W68_002246"
     CDS             3888..4166
                     /gene="rpsS"
                     /locus_tag="K0W68_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBI3073795.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4181..4513
                     /gene="rplV"
                     /locus_tag="K0W68_002247"
     CDS             4181..4513
                     /gene="rplV"
                     /locus_tag="K0W68_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBI3073796.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4531..5232
                     /gene="rpsC"
                     /locus_tag="K0W68_002248"
     CDS             4531..5232
                     /gene="rpsC"
                     /locus_tag="K0W68_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBI3073797.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5245..5655
                     /gene="rplP"
                     /locus_tag="K0W68_002249"
     CDS             5245..5655
                     /gene="rplP"
                     /locus_tag="K0W68_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBI3073798.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5655..5846
                     /gene="rpmC"
                     /locus_tag="K0W68_002250"
     CDS             5655..5846
                     /gene="rpmC"
                     /locus_tag="K0W68_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBI3073799.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5846..6100
                     /gene="rpsQ"
                     /locus_tag="K0W68_002251"
     CDS             5846..6100
                     /gene="rpsQ"
                     /locus_tag="K0W68_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBI3073800.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6265..6636
                     /gene="rplN"
                     /locus_tag="K0W68_002252"
     CDS             6265..6636
                     /gene="rplN"
                     /locus_tag="K0W68_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBI3073801.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6647..6961
                     /gene="rplX"
                     /locus_tag="K0W68_002253"
     CDS             6647..6961
                     /gene="rplX"
                     /locus_tag="K0W68_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBI3073802.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6976..7515
                     /gene="rplE"
                     /locus_tag="K0W68_002254"
     CDS             6976..7515
                     /gene="rplE"
                     /locus_tag="K0W68_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBI3073803.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7530..7835
                     /gene="rpsN"
                     /locus_tag="K0W68_002255"
     CDS             7530..7835
                     /gene="rpsN"
                     /locus_tag="K0W68_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBI3073804.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7869..8261
                     /gene="rpsH"
                     /locus_tag="K0W68_002256"
     CDS             7869..8261
                     /gene="rpsH"
                     /locus_tag="K0W68_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBI3073805.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8274..8807
                     /gene="rplF"
                     /locus_tag="K0W68_002257"
     CDS             8274..8807
                     /gene="rplF"
                     /locus_tag="K0W68_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBI3073806.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8817..9170
                     /gene="rplR"
                     /locus_tag="K0W68_002258"
     CDS             8817..9170
                     /gene="rplR"
                     /locus_tag="K0W68_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBI3073807.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9185..9688
                     /gene="rpsE"
                     /locus_tag="K0W68_002259"
     CDS             9185..9688
                     /gene="rpsE"
                     /locus_tag="K0W68_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBI3073808.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9692..9871
                     /gene="rpmD"
                     /locus_tag="K0W68_002260"
     CDS             9692..9871
                     /gene="rpmD"
                     /locus_tag="K0W68_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBI3073809.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9875..10309
                     /gene="rplO"
                     /locus_tag="K0W68_002261"
     CDS             9875..10309
                     /gene="rplO"
                     /locus_tag="K0W68_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBI3073810.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10317..11648
                     /gene="secY"
                     /locus_tag="K0W68_002262"
     CDS             10317..11648
                     /gene="secY"
                     /locus_tag="K0W68_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBI3073811.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11680..11796
                     /gene="rpmJ"
                     /locus_tag="K0W68_002263"
     CDS             11680..11796
                     /gene="rpmJ"
                     /locus_tag="K0W68_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBI3073812.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11943..12299
                     /gene="rpsM"
                     /locus_tag="K0W68_002264"
     CDS             11943..12299
                     /gene="rpsM"
                     /locus_tag="K0W68_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBI3073813.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12316..12705
                     /gene="rpsK"
                     /locus_tag="K0W68_002265"
     CDS             12316..12705
                     /gene="rpsK"
                     /locus_tag="K0W68_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBI3073814.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12739..13359
                     /gene="rpsD"
                     /locus_tag="K0W68_002266"
     CDS             12739..13359
                     /gene="rpsD"
                     /locus_tag="K0W68_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBI3073815.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13385..14374
                     /gene="rpoA"
                     /locus_tag="K0W68_002267"
     CDS             13385..14374
                     /gene="rpoA"
                     /locus_tag="K0W68_002267"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBI3073816.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14415..14798
                     /gene="rplQ"
                     /locus_tag="K0W68_002268"
     CDS             14415..14798
                     /gene="rplQ"
                     /locus_tag="K0W68_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBI3073817.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14905..15273
                     /locus_tag="K0W68_002269"
     CDS             14905..15273
                     /locus_tag="K0W68_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HBI3073818.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15284..15709
                     /gene="zntR"
                     /locus_tag="K0W68_002270"
     CDS             15284..15709
                     /gene="zntR"
                     /locus_tag="K0W68_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HBI3073819.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15765..15983
                     /gene="arfA"
                     /locus_tag="K0W68_002271"
     CDS             15765..15983
                     /gene="arfA"
                     /locus_tag="K0W68_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HBI3073820.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="K0W68_002272"
     CDS             complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="K0W68_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBI3073821.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="K0W68_002273"
     CDS             complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="K0W68_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBI3073822.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="K0W68_002274"
     CDS             complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="K0W68_002274"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBI3073823.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="K0W68_002275"
     CDS             complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="K0W68_002275"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBI3073824.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20215..20724)
                     /gene="def"
                     /locus_tag="K0W68_002276"
     CDS             complement(20215..20724)
                     /gene="def"
                     /locus_tag="K0W68_002276"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBI3073825.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20854..21978
                     /gene="dprA"
                     /locus_tag="K0W68_002277"
     CDS             20854..21978
                     /gene="dprA"
                     /locus_tag="K0W68_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBI3073826.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21950..22423
                     /locus_tag="K0W68_002278"
     CDS             21950..22423
                     /locus_tag="K0W68_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBI3073827.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22452..22994
                     /locus_tag="K0W68_002279"
     CDS             22452..22994
                     /locus_tag="K0W68_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HBI3073828.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22999..23571
                     /gene="tsaC"
                     /locus_tag="K0W68_002280"
     CDS             22999..23571
                     /gene="tsaC"
                     /locus_tag="K0W68_002280"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBI3073829.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23576..24394
                     /gene="aroE"
                     /locus_tag="K0W68_002281"
     CDS             23576..24394
                     /gene="aroE"
                     /locus_tag="K0W68_002281"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBI3073830.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24391..24648
                     /locus_tag="K0W68_002282"
     CDS             24391..24648
                     /locus_tag="K0W68_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBI3073831.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24624..25178)
                     /locus_tag="K0W68_002283"
     CDS             complement(24624..25178)
                     /locus_tag="K0W68_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBI3073832.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6492 a         6028 c         6682 g         6301 t
ORIGIN      
        1 cttctttaca aacattggta gatcagaatt tgccctggag cgcatcgctt aaagtcgggg
       61 acaaaaaatt gcctgttgtg gtcgttcgcg tcggtgaggc tagcgttgat gtcctcgtag
      121 gtcagcaaac gtggacgctg acacataaat ggtttgagtc ggtatggacg ggtgattatc
      181 ttctgttgtg gaaaatgtcg ccggaagggg agagtaccat tacgcgtgat agcagcgagg
      241 aagagattct ctggctggaa acgatgttaa accgtgcatt gcatatttcg acagaacctt
      301 cggcggagtg gcgtcccttg ttggtagaaa aaataaagca atttcaaaaa agccaccact
      361 tgaaaactga cggtgttgtt ggcttttcaa cgttagtaca tctctggcag gtggcagggg
      421 agagtgccta cttatatcgg gatgaagcaa atatttcccc ggaaacaaca gtgaagggga
      481 aatgacatgt ttgaattcta tatcgccgcg cgtgagcaga aagaaacagg tcaccctggt
      541 atcttctccc gacaaaaaca cagcaccata atatatgtca tttgtttatt gctgatttgc
      601 ctgtggtttg caggaatggt gttggttggt gggtatgcca ggcagctatg ggcgctttgg
      661 atagtaaaag cagaagtcac tgtagaggct gaaacgcccg cttttaaaca atcaacacaa
      721 cactatttct tcaaaaaaca gccactcccc gtcgtcgaat ccgtcgagga ggaagatgac
      781 ccagacgtcg cggttgagaa tgctccctct tcttctgaag acgaagaaaa tactgtaaaa
      841 gaaagcgacg agaaagccgg tttaagagag cgtgtcaaaa atgcgctgaa cgaacttgag
      901 agataacccg aaggctgttt actttactag caatacgctt gcgttcggtg gttaagtatg
      961 tataatgcgc gggcttgtcg tagttgacag caggttcaat ctgaacctca gttagccgaa
     1021 tttggctacc taacaatgct cccaatcggg gagctacgta agaacggtta cactctccca
     1081 tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat aaaataattg gagctctggt
     1141 ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat
     1201 caagcaaccg cggaaatcgt cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg
     1261 atcccgctgc cgacacgcaa agagcgcttc actgttctga tctccccgca cgtcaacaaa
     1321 gacgcgcgcg atcagtacga aatccgtact cacttgcgtc tggttgacat cgttgagcca
     1381 accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag
     1441 atcagcctgg gttaatcagg tcattgagcg attgagaggt tgaaacaatg attggtttag
     1501 tcggtaaaaa agtgggtatg acccgtatct tcacagaaga cggcgtttct atcccagtaa
     1561 ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa agacctggct aacgatggct
     1621 accgtgctat tcaggtgacc accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag
     1681 ctggccactt cgctaaagct ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg
     1741 ctgaaggcga agagttcact gtaggtcaga gcattagcgt tgaactgttt gctgaggtta
     1801 aaaaagttga cgtaactggc acctctaaag gtaaaggttt cgcaggtacc gttaagcgct
     1861 ggaacttccg tacccaggac gctactcacg gtaactcctt gtctcaccgc gttccgggtt
     1921 ctatcggtca gaaccagact ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga
     1981 tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt acgcgttgac gctgagcgca
     2041 acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac
     2101 cagctgtgaa ggcgtaagga gatagcaatg gaattagtat tgaaagacgc gcagagcgcg
     2161 ctgactgttt ccgaaactac cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt
     2221 gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa
     2281 gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca ccggccgtgc gcgttctggt
     2341 tctatcaaga gcccgatctg gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac
     2401 cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa
     2461 ctggtacgtc aggatcgtct gatcgttgtc gagaagttct ctgtagaagc gccgaaaact
     2521 aagctgctgg cacagaaact gaaagacatg gctctggaag atgtgctgat catcaccggt
     2581 gagctggacg aaaacctgtt cctggctgcg cgcaacctgc acaaggttga cgtacgcgat
     2641 gcaactggta tcgacccggt tagcctgatc gccttcgaca aagtcgtaat gactgctgat
     2701 gctgttaagc aagttgagga gatgctggca tgattcgtga agaacgtctg ctgaaggtgc
     2761 tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg
     2821 tactcaaagt tgctaaagac gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt
     2881 ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa agggaaagtt aaacgtcacg
     2941 gacagcgtat cggtcgtcgt agcgactgga aaaaagctta cgtcaccctg aaagaaggcc
     3001 agaatctgga cttcgttggc ggcgctgagt aagtcggagg agtaatacaa tggcagttgt
     3061 taaatgtaaa ccgacatctc cgggtcgtcg ccacgtagtt aaagtggtta accctgagct
     3121 gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa
     3181 caacaatggc cgtatcacca ctcgtcatat cggtggtggc cacaagcagg cttaccgtat
     3241 tgttgacttc aaacgcaaca aagacggtat cccggcagtt gttgaacgtc ttgagtacga
     3301 tccgaaccgt tccgcgaaca tcgcgctggt tctgtacaaa gacggtgaac gccgttacat
     3361 cctggcccct aaaggcctga aagctggcga ccagattcag tctggcgttg atgctgcaat
     3421 caaaccaggt aacaccctgc cgatgcgcaa catcccggtt ggttctactg ttcataacgt
     3481 agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc gctggtactt acgttcagat
     3541 cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt
     3601 agaagcagac tgccgtgcaa ctctgggcga agttggcaat gctgagcata tgctgcgcgt
     3661 tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc
     3721 gatgaacccg gtagaccacc cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc
     3781 ggtaactccg tggggcgttc agaccaaagg taagaagacc cgcagcaaca agcgtactga
     3841 taaattcatc gtacgtcgcc gtagcaaata attttagagg ataagccatg ccacgttctc
     3901 tcaagaaagg tccttttatt gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa
     3961 gcggagacaa gaagcccctg cgcacttggt cccgtcgttc aacgatcttt cctaacatga
     4021 tcggtttgac catcgctgtc cataatggtc gtcagcacgt tccggtattt gtgaccgacg
     4081 aaatggtcgg tcacaaactg ggtgaattcg caccgactcg tacttatcgc ggccacgctg
     4141 ctgataaaaa agcgaagaag aaataaggta ggaggaagag atggaaacta tcgctaaaca
     4201 tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa
     4261 gaaagtgtcg caggctctgg atattttgac ctacaccaac aagaaagcgg ctgtactggt
     4321 caagaaagtt ctggaatctg ccattgctaa cgctgaacac aacgatggcg ctgacattga
     4381 cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc
     4441 gcgtgcaaaa ggtcgtgcag atcgcatcct gaagcgcacc agccacatca ctgtggttgt
     4501 gtccgatcgc tgagactctg gagactagca atgggtcaga aagtacatcc taatggtatt
     4561 cgcttgggta ttgtaaaacc atggaactct acctggtttg cgaacaccaa agaattcgct
     4621 gacaacctgg acagcgattt taaagtacgt cagtacctga ctaaggaact ggctaaagcg
     4681 tctgtatctc gtatcgttat cgagcgtccg gctaagagca tccgtgtaac cattcacact
     4741 gctcgcccgg gtatcgttat cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc
     4801 gtagcggaca tcgctggcgt tcctgcacag atcaacatcg ccgaagttcg taagcctgaa
     4861 ctggacgcaa aactggttgc tgacagcatc acttctcagc tggaacgtcg cgttatgttc
     4921 cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa
     4981 gttgaagtta gcggccgtct gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa
     5041 ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact acaacacctc tgaagcgcac
     5101 accacttacg gtgtaatcgg cgttaaagtg tggatcttca aaggcgagat cctgggtggt
     5161 atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa
     5221 ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg
     5281 cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg ttagcttcgg cagcttcggt
     5341 ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct
     5401 atgacccgtg cagttaagcg tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg
     5461 atcactgaaa agccgctggc agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg
     5521 gttgccttga ttcagccggg taaagtcctg tatgaaatgg acggtgttcc ggaagagctg
     5581 gcccgtgaag cattcaagct ggcagcagcg aaactgccga ttaaaaccac ctttgtaact
     5641 aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca
     5701 ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc
     5761 agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt
     5821 tactgaacga gaaggcgggt gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt
     5881 tgttagcgac aaaatggaga aatccattgt tgttgctatc gaacgttttg tgaaacaccc
     5941 gatctacggt aaattcatca agcgtacgac caaactgcac gtacatgacg agaacaacga
     6001 atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc
     6061 ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa tacagtacac tctctcgata
     6121 cgaataaacg gctcagaaat gagccgttta ttttttctac ccatatcctt gaagcggtgt
     6181 tataatgccg cgccctcgat atggggattt ttaacgacct gattttcggg tctcagtagt
     6241 agttgacatt agcggagcac taaaatgatc caagaacaga ctatgctgaa cgtcgccgac
     6301 aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac
     6361 gcaggcgtag gcgacatcat caagatcacc atcaaagaag caattccgcg tggtaaggtc
     6421 aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg
     6481 gacggttctg tcattcgctt cgatggtaat gcttgtgttc ttctgaacaa caacagcgag
     6541 cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg agcttcgtag tgagaagttc
     6601 atgaaaatta tctctctggc accagaagta ctctaaggag cgaatcatgg cagcgaaaat
     6661 ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt
     6721 taagaatgtc ctgtcttccg gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca
     6781 tcagaagccg gttccggccc tgaaccaacc gggtggcatc gttgaaaaag aagccgctat
     6841 tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt
     6901 tagattcgaa gacggcaaaa aagtccgttt cttcaagtct aacagcgaaa ctatcaagta
     6961 atttggagta gtacgatggc gaaactgcat gattactaca aagacgaagt agttaaaaaa
     7021 ctcatgactg agtttaacta caattctgtc atgcaagtcc ctcgggtcga gaagatcacc
     7081 ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac tgctggataa cgcagcagca
     7141 gacctggcag caatctccgg tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca
     7201 ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag taactctgcg tggcgaacgc
     7261 atgtgggagt tctttgagcg cctgatcact attgctgtac ctcgtattcg tgacttccgt
     7321 ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag
     7381 atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc
     7441 attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc
     7501 ccgttccgca agtaaggtag ggttactaaa tggctaagca atcaatgaaa gcacgcgaag
     7561 taaaacgcgt agctttagct gataaatact tcgcgaaacg cgctgaactg aaagcgatca
     7621 tctctgatgt gaacgcttcc gacgaagatc gttggaacgc tgttctcaag ctgcagactc
     7681 tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgcccgc
     7741 atggtttcct gcggaagttc gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg
     7801 gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc accaattgaa tcacgggagt
     7861 aaagacagat gagcatgcaa gatccgatcg cggatatgct gacccgtatc cgtaacggtc
     7921 aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca
     7981 acgtgctgaa ggaagaaggt tttattgaag attttaaagt tgaaggcgac accaagcctg
     8041 aactggaact tactctgaag tatttccagg gcaaagctgt tgtagaaagc attcagcgtg
     8101 tcagccgccc aggtctgcgc atctataaac gtaaagatga gctgccgaaa gttatggcgg
     8161 gtctgggtat cgcagttgtt tctacctcta aaggtgttat gactgatcgt gcagcgcgcc
     8221 aggctggtct tggtggcgaa attatctgct acgtagccta atcggaggaa aaaatgtctc
     8281 gtgttgctaa agcaccggtc gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg
     8341 ttattacgat caaaggtaaa aacggcgagc tgactcgtac tctcaacgat gctgttgaag
     8401 ttaaacatgc agataatacc ctgaccttcg gtccgcgtga tggttacgca gacggttggg
     8461 cacaggctgg taccgcgcgt gccctgctga actcaatggt tatcggtgtt accgaaggct
     8521 tcactaagaa gctgcagctg gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga
     8581 ttaacctgtc tctgggtttc tctcatcctg ttgaccatca gctgcctgcg ggtatcactg
     8641 ctgaatgtcc gactcagact gaaatcgtgc tgaaaggcgt tgataagcag gtgatcggcc
     8701 aggttgcagc ggatctgcgc gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc
     8761 gttacgccga cgaagtcgtg cgtaccaaag aggctaagaa gaagtaaggt aacactatgg
     8821 ataagaaatt tgctcgtatc cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg
     8881 gcgcaactcg cctggtggta catcgtaccc cgcgtcacat ttacgcacag gtaattgcac
     8941 cgaacggttc tgaagttctg gtagctgctt ctactgtaga aaaagctatc gctgaacaac
     9001 tgaagtacac cggtaacaaa gacgcggctg cagctgtggg taaagctgtc gctgaacgcg
     9061 ctctggaaaa aggcatcaaa gatgtatcct ttgaccgttc cgggttccaa tatcatggtc
     9121 gtgtccaggc actggcagat gctgcccgtg aagctggcct tcagttctaa ggtagaggtg
     9181 taagatggct cacatcgaaa aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa
     9241 ccgcgtatct aaaaccgtta aaggtggtcg tattttctcc ttcacagctc tgactgtagt
     9301 tggcgatggt aacggtcgcg ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc
     9361 gatccagaaa gcgatggaaa aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg
     9421 cactctgcaa caccctgtta aaggtgttca cacgggttct cgcgtattca tgcagccggc
     9481 ttccgaaggt accggtatca tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg
     9541 ggttcataac gttctggcta aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc
     9601 aactattgat ggcctggaaa atatgaattc tccagaaatg gtcgctgcca agcgtggtaa
     9661 atccgttgaa gaaattctgg ggaaataaac catggcaaag actattaaaa ttactcaaac
     9721 ccgcagtgca atcggtcgtc tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg
     9781 tcgtattggt cacaccgtag agcgcgagga tactcctgct attcgcggta tgatcaacgc
     9841 ggtttccttc atggttaaag ttgaggagta agagatgcgt ttaaatactc tgtctccggc
     9901 cgaaggctcc aaaaaggcgg gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa
     9961 aaccggtggt cgtggtcaca aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg
    10021 tttcgagggt ggtcagatgc ctctgtaccg tcgtctgccg aaattcggct tcacttctcg
    10081 taaagcagcg attacagccg aagttcgtct gtctgacctg gctaaagtag aaggcggtgt
    10141 tgtagacctg aacacgctga aagcggctaa cattatcggt atccagatcg agttcgcgaa
    10201 agtgatcctg gctggcgaag taacgactcc ggtaactgtt cgtggcctgc gtgttactaa
    10261 aggcgctcgt gctgctatcg aagctgctgg cggtaaaatc gaggaataag tagcagatgg
    10321 ctaaacaacc gggattagat tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca
    10381 gactgctgtt tgttatcggt gcgctgattg tgttccgtat tggctctttt attccgatcc
    10441 ctggtattga tgccgctgta cttgccaaac tgcttgagca acagcgaggc accatcattg
    10501 agatgtttaa catgttctct ggtggtgctc tcagccgtgc ttctatcttt gctctgggga
    10561 tcatgccgta tatttcggcg tcgatcatta tccagctgct gacggtggtt cacccaacgt
    10621 tggcggaaat taagaaagaa ggggagtctg gtcgtcgtaa gatcagccag tacacccgct
    10681 acggtactct ggtgctggca atattccagt cgatcggtat tgctactggt ctgccgaata
    10741 tgcctggtat gcaaggcctg gtgattaacc cgggctttgc attctacttc accgctgttg
    10801 taagtctggt cacaggaacg atgttcctga tgtggttggg cgaacagatt actgaacgag
    10861 gtatcggcaa cggtatttca atcattatct tcgtcggtat tgtcgcggga ctcccgccag
    10921 ccattgccca tactatcgag caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt
    10981 tggttgcagt attagtattt gcagtgacgt tctttgttgt atttgttgag cgtggtcaac
    11041 gccgcattgt ggtaaactac gcgaaacgtc agcaaggtcg tcgtgtttat gctgcacaga
    11101 gcacacattt accgctgaaa gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca
    11161 gtattattct gttcccggcg accatcgcgt catggttcgg gggcggtact ggttggaact
    11221 ggctgacaac aatttcgctg tatttgcagc ctgggcaacc gctttatgtg ttactctatg
    11281 cgtctgcaat catcttcttc tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa
    11341 cagcagataa cctgaagaag tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa
    11401 cggcgaagta tatcgataaa gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta
    11461 cctttatctg cctgatcccg gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg
    11521 gtgggacctc actgcttatc gttgttgtcg tgattatgga ctttatggct caagtgcaaa
    11581 ctctgatgat gtccagtcag tatgagtctg cattgaagaa ggcgaacctg aaaggctacg
    11641 gccgataatt ggtcgcccga gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc
    11701 aagaaattat gccgtaactg caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc
    11761 agtgccgagc cgaagcataa acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa
    11821 agttgggttg agctggctag attagtcagc caatcttttg tatgtctgtg cgtttccatt
    11881 tgagtatcct gaaaacgggc ttttcagcat ggaacgtaca tattaaatag taggagtgca
    11941 tagtggcccg tatagcaggc attaacattc ctgatcataa gcatgccgta atcgcattaa
    12001 cttcgattta tggcgtcggc aagacccgtt ctaaagccat cctggctgca gcgggtatcg
    12061 ctgaagatgt taagatcagt gagctgtctg aaggacaaat cgacacgctg cgtgacgaag
    12121 ttgccaaatt tgtcgttgaa ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc
    12181 tgatggatct tggttgctat cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc
    12241 agcgtaccaa gaccaacgca cgtacccgta agggtccgcg caaaccgatc aagaaataat
    12301 cggggtgatt gaataatggc aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa
    12361 gtctctgacg gcgtggctca tatccatgct tctttcaaca acaccatcgt gactatcact
    12421 gatcgtcagg gtaacgcgtt gggttgggca acagccggtg gttccggttt ccgtggttct
    12481 cgcaaatcca ctccgtttgc agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa
    12541 gaatacggca tcaagaatct ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct
    12601 actattcgtg ctctgaacgc cgcaggtttc cgcatcacta acattactga tgtgactccg
    12661 atccctcata acggttgtcg tccgccgaaa aaacgtcgcg tataacgcct cgttttccag
    12721 gtttgttgga gaaagaaaat ggcaagatat ttgggtccta agctcaagct gagccgtcgt
    12781 gagggcaccg acttattcct taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt
    12841 gaacaagctc ctggccagca cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag
    12901 ttgcgtgaaa agcaaaaagt tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac
    12961 tactacaaag aagcagcacg tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg
    13021 gaaggtcgtc tggacaacgt tgtataccgt atgggcttcg gtgccactcg tgcagaagca
    13081 cgtcagctgg ttagccataa agcaattatg gtaaacggtc gtgttgttaa catcgcttct
    13141 tatcaggtta gtccgaatga cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc
    13201 gtgaaagccg ctctggagct ggctgagcag cgtgaaaagc caacctggct ggaagttgat
    13261 gctggcaaga tggaaggtac gtttaagcgt aagccggagc gttctgatct gtctgcggac
    13321 attaacgaac acctgatcgt cgagctttac tccaagtaaa gcttagtacc aaagagagga
    13381 cacaatgcag ggttctgtga cagagtttct aaaaccgcgc ctggttgata tcgagcaagt
    13441 gagttcgacg cacgccaagg tgacccttga gcctttagag cgtggctttg gccatactct
    13501 gggtaacgca ctgcgccgta ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt
    13561 tgagattgat ggtgtactac atgagtacag caccaaagaa ggcgttcagg aagatatcct
    13621 ggaaatcctg ctcaacctga aagggctggc ggtgagagtt cagggcaaag atgaagttat
    13681 tcttaccttg aataaatctg gcattggccc tgtgactgca gccgatatca cccacgacgg
    13741 tgatgtcgaa atcgtcaagc cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc
    13801 tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat
    13861 tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc
    13921 tgtggagcgt attgcctaca atgttgaagc agcgcgtgta gaacagcgta ccgacctgga
    13981 caagctggtc atcgaaatgg aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg
    14041 tgcggcaacc attctggctg aacaactgga agctttcgtt gacttacgtg atgtacgtca
    14101 gcctgaagtg aaagaagaga aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga
    14161 tctggaattg actgtccgct ctgctaactg ccttaaagca gaagctatcc actatatcgg
    14221 tgatctggta cagcgtaccg aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc
    14281 tcttactgag attaaagacg tgctggcttc ccgtggactg tctctgggca tgcgcctgga
    14341 aaactggcca ccggcaagca tcgctgacga gtaaccggat cacaggttaa ggttttactg
    14401 agaaggataa ggtcatgcgc catcgtaaga gtggtcgtca actgaaccgc aacagcagcc
    14461 atcgccaggc tatgttccgc aatatggcag gttcactggt tcgtcatgaa atcatcaaga
    14521 cgactctgcc taaagcgaaa gagctgcgcc gcgtagttga gccgctgatt actcttgcca
    14581 agactgatag cgttgctaat cgtcgtctgg catttgcccg tactcgtgat aacgagatcg
    14641 tggcaaaact gtttaacgaa ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc
    14701 gtattctgaa gtgtggcttc cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg
    14761 ttgatcgttc agagaaagca gaagctgctg cagagtaatc tgaagcaatg taaaaaaacc
    14821 cgccccggcg ggttttttta tacccgtagt atccccactt atctacaata gctgtactct
    14881 ttttgttcat cccctggagt atttatgtgg ttacttgacc agtgggcaga gcgccatata
    14941 gcagaagcgc aagcgaaagg tgagtttgat aacctggcag gtagcggcga accattgata
    15001 ctggatgatg attctcacgt gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat
    15061 gccggttgct taccgccaga acttgagcaa cggagagaag caattcagct tctggatatt
    15121 ctcaaaggta tccgtcacga tgatccgcaa tatcaagagg ttagccgtcg attgtcatta
    15181 ctggaattga agctgcgaca agctggattg agtaccgatt ttttacgcgg cgattatgct
    15241 gacaagttgt tggacaaaat caacgataac tagtggagta tgtatgtatc gcattggtga
    15301 gctggcaaaa atggcggaag taacacctga cacgattcgt tattacgaaa aacagcagat
    15361 gatggagcat gaagtgcgta ctgaaggcgg gtttcgcctg tataccgaaa gcgatctcca
    15421 gcgattgaaa tttatccgcc atgccagaca actaggtttc agtctggagt cgatccgcga
    15481 gttgctgtcg atccgcatcg atcctgaaca ccatacctgt caggagtcaa aaggcattgt
    15541 gcaggaaaga ttgcaggaag tcgaagcacg gatagccgag ttgcagagta tgcagcgttc
    15601 cttgcaacgc cttaacgatg cctgttgtgg gaccgcccat agcagtgttt attgctcgat
    15661 tcttgaagct cttgaacaag gggcgagtgg cgttaagagt ggctgttgat tttttgcact
    15721 gacaggatta cactcgcgcc gttaaataac caactggagt ttttatgagc cgatatcagc
    15781 atactaaagg gcagataaag gataatgcga tagaagcatt actacatgat cccttattcc
    15841 gacagcgagt agagaaaaat aagaaaggga aaggcagtta catgcgaaaa ggaaaacatg
    15901 gcaatcgggg gaactgggag gccagtggca agaaagtaaa tcactttttt accactggtc
    15961 ttctgctttc aggcgcttgt taagagcggt tattctgctc tttcagcaaa tcacgaattt
    16021 ctgccagtaa tacttcttct ttagttggtg caggtgcggc agctggttct tcttttttcc
    16081 gattcagttt gttgattagc ttaatcgcca taaagatggc aaaggccaca atcagaaaat
    16141 caaagacgtt ttgaatgaag acaccgtaat gcatcacaac agcagggata tccccctgcg
    16201 catcgcgtag cgtgacagca aactgtttaa agtcgatccc gccaattaat aagcccagag
    16261 gtggcatgat gatatcggca accagtgaag atacaatctt cccgaatgcc gcaccgataa
    16321 tgacacccac cgccaaatcc accacgttcc cgcgcatcgc aaattcgcga aattctttaa
    16381 taatgctcat gttattctcc ctatgaagcc gacaaccata agtctaacaa atgttaagcc
    16441 attttcctgc caggaatgaa tttaattaat ccttgataat caaagggaaa taataaggcg
    16501 tcattagacg ccttattaat tacaagaaga aagggcttgg ctggaagagt ctttcgacgt
    16561 cggtaataaa ctttttatct gtgaggaaca taattacgtg atcgccttgc tcaatgcgca
    16621 gattgtcatt ggcaatcatc acgtcgtttc cacgtaccac cgctccaata atcgttcctg
    16681 gcggtagctt gatttcgtca atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt
    16741 gagcaacggc ttcaatagct tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat
    16801 ctgcttttcg cacatggcta agcaacgcag aaatagttgc ttgttgtggt gaaattgcaa
    16861 tatcgatcac gctcccctga accagatcca cataagcgcg acgctggatc aataccatca
    16921 cctttttcgc acccatacgt ttggcaagca tggcggacat gatattggcc tcgtcatcgt
    16981 tggtgacggc aataaacaga tcaacttgat cgatatgttc ttcggccagc agttcttgat
    17041 ccgacgcatc accaaaaaag acgatcgtat tctgcaactt ttccgccagt tcggcagcgc
    17101 gctgctgatt acgttcgatg agtttaacgc tgtaatcttt ttccagacga cgcgccagcc
    17161 ctgcaccgat attaccgcca ccaaccagca tgatccgctt atacggtttt tccagtcgct
    17221 gtaattcact catcaccgcg cggatatgct gtgaagcggc aataaagaac acttcatcac
    17281 cagcttcaac aatggtcgaa ccttgtggac gaatggggcg atcgtggcgg aaaattgctg
    17341 ccacacgagt atcgatatgt ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca
    17401 gcgggccgcc ataataggct ttaactacag cgaggctgac tttaccctca gcgaagttca
    17461 ccacctgcaa tgcgcctgga tactcaatca gtcggtaaat attatcgata accaactgct
    17521 ctggtgcgat cagatgatca atcggtacag catctgaatg aaatagctta tcggcatcgc
    17581 gtacgtagtc tggtgagcgg atgcgagcga tgcgattagg ggtgttgaaa agtgagtagg
    17641 ctacctggca ggcaaccata ttggtttcat ctgaactggt tacagcaacc agcatatcgg
    17701 cgtcgtcggc acctgcctcc cgcaatacgc gtggatgaga gccatgcccc tgcacgactc
    17761 gcaggtcaaa tttatcctgc aaggtccgca gacgctcacc gttggtatcg acaacagtaa
    17821 tatcgttgtt ctcgccaacc aggttttccg ccagtgtgcc gccaacctgg ccggcaccca
    17881 gaatgataat tttcatcagt ctcgacccgt tatctcatca ctttttgatt agcttagcgt
    17941 aaaagaagcc gtcgccctct tcggcaccag gcagattttg tttacccggt tgctctggtg
    18001 ttcctgtttc gcaaagttcg gcatcagcgg tacgttgcaa aaaagcttta atctgcaggc
    18061 tattctcttc cggtaacacc gaacaggtgg catagaccag ggttccaccg gattttaaat
    18121 gcggccaaat ggcgtcgaga atttcagact gcaattgcgc gagttccggg atatcgcgat
    18181 cgcgacgtaa ccatttaata tccggatggc gacgaatcac ccccgttgct gaacaaggcg
    18241 catctaataa aatgcgatca aactgttgct cgccacacca ttgggaaggg taacggccat
    18301 caccttgttt cacggtcgcc ttcataccaa ggcgttttaa attgtcgtga acgcgagaga
    18361 ggcgctgttc gtcaatatca accgcaacaa cctgcgcttc tggtgccacc tcaaggatat
    18421 gcgttgtttt accgccgggg gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg
    18481 caagccaggt catgcaacct tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa
    18541 aaccaggtag cgcatgaaca ggtgcaggtg tttccagccg tacagcatca gggtaatccg
    18601 catgcgggaa acctttcatt cctgcttcat ccaacaatgc aagccagctg tcgcgggaat
    18661 gatgcgtacg attaacgcgc agccacattg gcggacgctg gttattggct tcgacgatgg
    18721 attgccactg ctctggatac gctttttgca gacgcttcag caaccaggaa ggatgcagat
    18781 aacgtgcatc actggtatta aactcggcta ataactcttc ttgctgacgc tggaactggc
    18841 gtaatacacc gttaatcaac cctttaagtt gcggacgctt aattgcgata gcgccttcaa
    18901 ccgtttcagc cagcgcagca tgaggtggaa tgcgggtata aagcagttga tacaaaccaa
    18961 ccataatcag gtaatgcaca gtgcgctgtt tgccggtcat cggacgggcc attaacttat
    19021 taatcagcca gtctaactgc gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa
    19081 gtgctttgtc tttatcagaa actttttgct ggagcggtgg cagaatgttg cttaatgatt
    19141 gcccttgctc gacgacttgt tcaacggcct gggccgccat gctacgtaaa ttacgttgtt
    19201 ttttcataag tataaaaatg cccggtaaga ccgggcttag aagagtggac tatcaggcca
    19261 gacggttgcc cggaacaaac cattcccgac gagaattcag gaggtcttgc gcgctcatcg
    19321 ctttcttacc cgcaggttgt aacgagagca ggttcaggat gccatcacca gtcgcaacct
    19381 gaatgccttg tttgttggct tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa
    19441 tgaccgacgc tttccagact ttaaccggtt gtccttcaat ttccagccag ctcattggcc
    19501 atgggttgaa agcgcgaatg cagcgttcaa gctgcgctgc cgaaagcgac cagtcaatac
    19561 gcgcttcttc tttactcaac ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg
    19621 gtttcgccgt gccgtctgct agttgtttca acgtggtgat tagcccttgt gggccaagct
    19681 ctgccagctt gtcgtacagc gtaccactgg tatcttctgc agtaatcggg caggagagct
    19741 tatagagcat atcaccggtg tctaaaccga catccatttg cataatggtc acaccagttt
    19801 ctgcatcacc cgcccatagt gagcgttgga ttggtgcagc accgcgccag cgtggcagca
    19861 gtgaaccatg aacgttgata cagccaagac gcggcatctc cagcactgct ttaggcagaa
    19921 ttaagccata ggcgacgacg accataacat cagcctgcag atcggcgacc agttgctggc
    19981 tttcttgtgg acgcagggaa acaggttgaa aaacgggcag acctttttcc tcagccagaa
    20041 ctttaaccgg gctgggcatc agttttttac cgcgtcctgc cggtcggtct ggctgggtga
    20101 acacgccaac gacgttatga ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt
    20161 caggtgtacc cgcaaaaata atacgtagtg attctgacac gttagttctt atccttaagc
    20221 ccgggctttc agacgatcta gtttttcaac tttctgacga atacgttgtt gtttcagcgg
    20281 tgacagataa tccataaaca gtttgccgac caggtgatcc atctcatgct gaatacagat
    20341 ggctaacagc ccctctgctt ccagttcaaa tggtttaccg tcgcggtcaa gggcgcgaat
    20401 tttaactttc tctgcgcgcg gcactaaagc acgttgttca gggatcgaca ggcaaccttc
    20461 ttcaatgcct gtttcgccgc ttttttctaa aagctccgga ttgattaaca ccagccgttc
    20521 gtcacggttt tccgaaacat caataacaat gatacgttga tggatatcaa cctgggttgc
    20581 cgccaggcca ataccttctt ctgcgtacat cgtctcgaac atatcatcga cgatacgctg
    20641 tatttctgca ttcacttctt ctaccggttt agcaactttg cgaagccgct cgtccggaat
    20701 atgtaacact tgcaaaactg acataaatct ccagagatgt gttcaggggt tagaaagatt
    20761 atttcttcta ttctagacaa atccccctct gattgacagc atcactgacc aatcgcaaag
    20821 attgctaagg ctgcttatgg cagggagata aggatggtcg atacagaaat ttggctgcgt
    20881 ttaatgagta tcagcagctt gtacggcgat gatatggtcc gtatagctca ctggctggca
    20941 aaacagtcgc aaattgatgc ggttggattg cagcaaacag ggcttacatt gcggcaggca
    21001 caacgctttc tttcatttcc gcgaaagagt atcgaaagct cactttgttg gttggagcaa
    21061 cccaaccatc atttaatccc tgcggacagc gaattttatc ctcctcaact tctggtgacg
    21121 acagattacc ccggcgcact gtttgttgaa ggagaactgc acgcgctgca ttcatttcag
    21181 cttgccgtag tggggagtcg ggcgcattca tggtatggcg agcgatgggg acggttattt
    21241 tgcgaaactc tggcgacgcg tggagtgaca attacgagtg gactggcgcg tggaatcgat
    21301 ggtgtggcgc ataaagctgc cttacaggta aatggcgtca gcattgctgt attggggaat
    21361 ggacttaata ccattcatcc ccgccgccat gcccgactgg ctgccagtct gcttgaacat
    21421 ggcggagctc tcgtctcgga atttcccctc gatgttccac cccttgctta caatttccca
    21481 cgaagaaatc gcattatcag tggtctaagt aaaggtgtac tggtggtgga agcggctttg
    21541 cgcagtggtt cgctggtgac agcacgttgt gcgcttgagc agaggcgtga agtttttgcc
    21601 ttgccaggtc caatagggaa tccgggaagc gaagggcctc actggttaat aaaacaaggt
    21661 gcgattcttg tgacggaacc ggaagaaatt ctggaaaact tgcaatttgg attgcactgg
    21721 ttgccagacg cccctgaaaa ttcattttat tcaccagatc agcaagacgt ggcattgcca
    21781 tttcctgagc tcctggctaa cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa
    21841 cgtgccggcc aacctgtgcc agaggtagtt actcaactac tcgaactgga gttagcagga
    21901 tggatcgcag ctgtacccgg cggctatgtc cgattgagga gggcatgcca tgttcgacgt
    21961 actaatgtat ttgtttgaaa cctatattca cactgaagct gagttgcgtg tggatcaaga
    22021 caaacttgaa caggatctta ccgacgcagg ttttgagcga gaagatatct acaatgccct
    22081 gctatggctg gaaaaacttg ctgattatca ggaagggttg gcagaaccga tgcaactggc
    22141 ctcggatcct ctctccatgc gtatttatac accggaagag tgtgaacgac tggatgccag
    22201 ctgccgtggg tttctgcttt tccttgagca gattcaggtg ctcaaccttg aaactcgtga
    22261 aatggtgata gagcgagtgc tcgcgctgga taacgcagag ttcgaactgg atgatctgaa
    22321 atgggtgatc ctgatggtgt tgttcaacat cccggggtgc gaaaatgcgt accagcaaat
    22381 ggaagaatta ctctttgaag tgaatgaagg tatgctgcat taatcatttc tttaattcag
    22441 cataagttgt tatggcaaaa tcagcactgt tcacggtgcg taataatgag tcctgcccaa
    22501 agtgcggggc tgaactggtt attcgatccg ggaaacacgg tccgtttctt ggatgctcac
    22561 agtatccggc gtgtgactac gtccgtcttc tgaaatcttc agcggatgga catattgtca
    22621 aagttctgga ggggcaggtt tgccctgcat gtggcgcaaa tctggtatta cgccagggac
    22681 gctttggtat gtttattggt tgcagtaact accctgaatg cgaacatacc gaacttatcg
    22741 ataaaccgga cgaaacagca attacatgcc cccaatgtcg gacgggccat ctggtccagc
    22801 gccgctcccg ttatggcaaa acatttcact cctgtgatcg ctacccggag tgtcaatttg
    22861 ccattaactt caaaccaata gctggagaat gccctgagtg tcattatccg ctactcatcg
    22921 aaaagaaaac cgcgcagggt gtaaaacact tttgtgccag taaacaatgt ggaaagccgg
    22981 tttcggcgga ataataacgt gaataataac ctgcaaggag acgctatcgc agctgcgata
    23041 gatgtcctca atgaagaacg tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg
    23101 tgcgatcctg atagcgaaac agcagtgatg agactgttgg agttaaaaca gcgtccggtt
    23161 gataaggggc tgattttaat cgcggcaaat tacgagcagc ttaaacccta tattgatgac
    23221 accatgctga ctgatgcgca gcgtgaaacc attttttccc gctggccagg tcctgtcacc
    23281 tttgtctttc ccgcgcctgc gacaacaccg cgctggttga cgggccgctt tgattcgctt
    23341 gctgtacgag tcaccgacca tccgttggtg gttgctttgt gccaggctta tggtaaaccg
    23401 ctggtttcta ccagtgccaa cttgagtgga ttgccacctt gtcgaacagt agacgaagtt
    23461 cgcgcacaat ttggcgcggc gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat
    23521 ccttcagaaa tccgcgatgc cctgacgggt gaactgtttc gacaggggta acataatgga
    23581 aacctatgct gtttttggta atccgatagc ccacagcaaa tcgccattca ttcatcagca
    23641 atttgctcag caactgaata ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga
    23701 tttcatcaac acactgaacg ctttctttcg tgctggtggt aaaggtgcga atgtgacggt
    23761 gccttttaaa gaagaggctt ttgccagagc ggatgagctt actgaacggg cagcgttggc
    23821 tggtgcagtt aataccctca agcggttaga agatggacgc ctgctgggtg acaataccga
    23881 tggtgtaggc ttgttaagcg atctggaacg tctgtctttt atccgccctg gtttacgtat
    23941 tctgcttatc ggcgctggtg gagcatctcg cggcgtacta ctgccacttc tttccctaga
    24001 ctgtgcggtg acaatcacta atcggacggt atcccgcgcg gaagagttgg ctaaattgtt
    24061 tgcgcacact ggcagtattc aggcgttggg tatggacgaa ctggaaggtc atgagtttga
    24121 tctcattatt aatgcaacat ccagtggcat cagtggtgat attccggcga tcccgtcatc
    24181 gctcattcat ccaggcattt attgctatga catgttctat cagaaaggaa aaactccttt
    24241 tctggcatgg tgtgagcagc gaggctcaaa gcgtaatgct gatggtttag gaatgctggt
    24301 ggcacaggcg gctcatgcct tccttctttg gcatggcgtt ctgcctgatg tagaaccggt
    24361 catcaaacta ttgcaacagg aattgtcagc gtgaatcagg ccatccagtt tccggatagg
    24421 gaagagtggg acgagaataa aagatgtgta tgttttcctg ctctcgtgaa tggtatgcaa
    24481 ctgacatgtg cgatctctgg cgagagtctg gcgtatcgct ttactggagg tacgccagaa
    24541 cagtggttag cgagttttcg tcagcatcgc tgggacctgg aagaagaagc ggaaaactta
    24601 attcaggaac aaagtgaaga tgatcaaggt tgggtctggt taccttgatc cagatattcg
    24661 tccttccatt tcacgtagtt atttgcggaa tagcgtaacc cagccttctc ttcatcactt
    24721 aacgggcgga tctgtttgac gggactaccg agatacagat atccgctctc cagccgttta
    24781 ttttgtggga ccaggctacc cgcaccaatc atcacatcat cttctactat tgcgccatca
    24841 agtaaaattg agcccatccc aaccaaaact cgattgccaa tggtgcagcc gtggagcatc
    24901 accttgtgac caacagtgac atcttcgcca atggttaatg ggttgccatc tgggttgtac
    24961 gaggatttat gagtgacatg caacatactg ccatcctgga tattggtgcg tgctccgatc
    25021 tgtacataat gtacatctcc acgaatcaca acgagcggcc agatccccac atcatcagcc
    25081 agacgaacgt caccaatcac gacgctgcta tcgtcgatca ttacgcgctg gccgatttgt
    25141 ggaaaaagat cgcggtatgg gcgtaaaaca tcagacatac tcacctcaac aataaatgat
    25201 ttcctaatga ctttgggtgc attattagcc ttgtgcaagt cttttagtat gcaaaaaagc
    25261 accgttttgt gtgcgaatgc agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc
    25321 aaaaaagtac ttgtgcaaaa aattgggatc cctataatgc gcctccgttg agacgacaac
    25381 gtgaaacact tcacaggatg gtcggaacaa cgaagagaaa aaattctgaa attcagggtt
    25441 gactctgaaa gaggaaagcg taatatacgc cacctcgcga cagtgagctg aaagccgcgt
    25501 cgc
//
LOCUS       DAETQN010000056        25431 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.056, whole genome shotgun sequence.
ACCESSION   DAETQN010000056 DAETQN010000000
VERSION     DAETQN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.056"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            124..447
                     /gene="soxS"
                     /locus_tag="K0W68_002284"
     CDS             124..447
                     /gene="soxS"
                     /locus_tag="K0W68_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBI3073833.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(450..2036)
                     /gene="pdeC"
                     /locus_tag="K0W68_002285"
     CDS             complement(450..2036)
                     /gene="pdeC"
                     /locus_tag="K0W68_002285"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HBI3073834.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2466..2746
                     /locus_tag="K0W68_002286"
                     /pseudo
     CDS             2466..2746
                     /locus_tag="K0W68_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2845..3381)
                     /gene="ssb1"
                     /locus_tag="K0W68_002287"
     CDS             complement(2845..3381)
                     /gene="ssb1"
                     /locus_tag="K0W68_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBI3073835.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3635..6457
                     /gene="uvrA"
                     /locus_tag="K0W68_002288"
     CDS             3635..6457
                     /gene="uvrA"
                     /locus_tag="K0W68_002288"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBI3073836.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6492..6848)
                     /locus_tag="K0W68_002289"
     CDS             complement(6492..6848)
                     /locus_tag="K0W68_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBI3073837.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6852..7268)
                     /locus_tag="K0W68_002290"
     CDS             complement(6852..7268)
                     /locus_tag="K0W68_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBI3073838.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7379..8092)
                     /gene="aphA"
                     /locus_tag="K0W68_002291"
     CDS             complement(7379..8092)
                     /gene="aphA"
                     /locus_tag="K0W68_002291"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBI3073839.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8494..8716
                     /locus_tag="K0W68_002292"
                     /pseudo
     CDS             8494..8716
                     /locus_tag="K0W68_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9220..10413)
                     /gene="tyrB"
                     /locus_tag="K0W68_002293"
     CDS             complement(9220..10413)
                     /gene="tyrB"
                     /locus_tag="K0W68_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBI3073840.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10666..11745)
                     /gene="alr"
                     /locus_tag="K0W68_002294"
     CDS             complement(10666..11745)
                     /gene="alr"
                     /locus_tag="K0W68_002294"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBI3073841.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(11798..13213)
                     /gene="dnaB"
                     /locus_tag="K0W68_002295"
     CDS             complement(11798..13213)
                     /gene="dnaB"
                     /locus_tag="K0W68_002295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBI3073842.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13296..14279
                     /locus_tag="K0W68_002296"
     CDS             13296..14279
                     /locus_tag="K0W68_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBI3073843.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(14445..14687)
                     /gene="pspG"
                     /locus_tag="K0W68_002297"
     CDS             complement(14445..14687)
                     /gene="pspG"
                     /locus_tag="K0W68_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBI3073844.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(14821..15858)
                     /gene="dusA"
                     /locus_tag="K0W68_002298"
     CDS             complement(14821..15858)
                     /gene="dusA"
                     /locus_tag="K0W68_002298"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBI3073845.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(16221..17206)
                     /locus_tag="K0W68_002299"
                     /pseudo
     CDS             complement(16221..17206)
                     /locus_tag="K0W68_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            17524..18039
                     /gene="zur"
                     /locus_tag="K0W68_002300"
     CDS             17524..18039
                     /gene="zur"
                     /locus_tag="K0W68_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBI3073846.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(18081..18290)
                     /locus_tag="K0W68_002301"
     CDS             complement(18081..18290)
                     /locus_tag="K0W68_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBI3073847.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(18406..19731)
                     /gene="dinF"
                     /locus_tag="K0W68_002302"
     CDS             complement(18406..19731)
                     /gene="dinF"
                     /locus_tag="K0W68_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBI3073848.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(19804..20412)
                     /gene="lexA"
                     /locus_tag="K0W68_002303"
     CDS             complement(19804..20412)
                     /gene="lexA"
                     /locus_tag="K0W68_002303"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBI3073849.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(20522..20890)
                     /gene="dgkA"
                     /locus_tag="K0W68_002304"
     CDS             complement(20522..20890)
                     /gene="dgkA"
                     /locus_tag="K0W68_002304"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBI3073850.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            21061..23484
                     /gene="plsB"
                     /locus_tag="K0W68_002305"
     CDS             21061..23484
                     /gene="plsB"
                     /locus_tag="K0W68_002305"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBI3073851.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(23639..24511)
                     /gene="ubiA"
                     /locus_tag="K0W68_002306"
     CDS             complement(23639..24511)
                     /gene="ubiA"
                     /locus_tag="K0W68_002306"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBI3073852.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(24524..25021)
                     /gene="ubiC"
                     /locus_tag="K0W68_002307"
     CDS             complement(24524..25021)
                     /gene="ubiC"
                     /locus_tag="K0W68_002307"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBI3073853.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(25244..>25431)
                     /locus_tag="K0W68_002308"
     CDS             complement(25244..>25431)
                     /locus_tag="K0W68_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073854.1"
                     /translation="MLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALF
                     PIFTASWRATRIKNIGI"
BASE COUNT         6578 a         6543 c         6090 g         6220 t
ORIGIN      
        1 gttagcagcg ctttaatgcg gggtaatttc ttttccataa atcgctttac ctcaagttaa
       61 cttgaggaat tatactcccc aacagatgaa ttaacgaact gaacactgaa aagaggcaga
      121 tttatgtccc atcagaaaat tattcaggat cttatcgcat ggattgacga gcatattgac
      181 cagccgctta acattgatgt agtcgcaaaa aaatcaggct attcaaagtg gtacttgcaa
      241 cgaatgttcc gcacggtgac gcatcagacg cttggcgatt acattcgcca acgtcgcctg
      301 ttactggccg ccgttgagtt gcgcaccacc gagcgtccga tttttgatat cgcaatggac
      361 ctgggttatg tctcgcagca gaccttctcc cgcgttttcc gtcggcagtt tgatcgcact
      421 cccagcgatt atcgccaccg cctgtagttt tattgcccgc gcgttaactc ccgcgcgggt
      481 aattgctcca tccattgcat aaacacctgc ggcggcatcg ccttcgcaaa aaaccatccc
      541 tggcaatagc gcacgccgcg tttgcgcagc cagttaacct gctcctcagt ttcgacgcct
      601 tcagcgatcg tttttaaccc caggctgtgc gccagctcga tgatgtgttc cgcaatcaaa
      661 tgactggttt tgtgggtggt cagcgtttca acaaacgatt tatcgatttt caaaatatcg
      721 acattcaatg atttaaggtt atgcaagtta gagtagccaa taccaaaatc atcaattgcc
      781 acttcgtaac cagcctggcg gaaagccaga ataatcggcg tcattttttc gacatcaaga
      841 aatgcatgct cagtcacttc aaatttaatt tgctgcggac gcaccgcgta ttgctctgtt
      901 ttctgattga ttcgcgctat caaccgtgac gtatgaaaat cggaggccga caggttaata
      961 gaaacatagc gatcggcatg tgttgccaag tacgcgccca gatcgcggaa gacattatca
     1021 ataacataat cagttatctg ttctatcatc ccctcctttt ctgccagcgg aataaactct
     1081 gccggattca ttatttgccc ctgctcacca agccaacgta acaacgcttc agcgccgata
     1141 catttttctg ttttgatatc gattattggc tggtaataaa gacaaagttg atgtttttcg
     1201 agggcgcgtt gcaatttacg tttgggagat aaatagtttt gtcgaatacg tagccagagt
     1261 aatagcagaa caagactccc caaaataccg gcgggcaacg caaaaataag atgattataa
     1321 aaatgggtta taagacgttg atatgaagtc gcaacaatgg ctgcaattgg gcgttttgtc
     1381 gaataaactg tcgcatataa atagccattt ctttgtacgg ttaaatcctt caaatgaatc
     1441 agcggagaaa acgttgctgc cgaggcctct ttgcttaacg agaaaaaggt tttcgtcacc
     1501 gtatcataca caccccattg caatgtcggg tcatcagaca tcacttcact ccagaagaga
     1561 gggttgataa ccgccacata atttccccgc tgcatatagg tcattttata gccagagaaa
     1621 aaaggcgtat cgcggtaata atagatagaa acgttaggtt cacgcttata atcggccggt
     1681 gcaatcgtat agccgtttac aggcgctatc agcgatgagc ataaaaaatg gttatcacgg
     1741 gcatagatca attcattaat ataaagatag ccacgaataa tattcaacat tcgcttttga
     1801 tgggctggag tgcaaacttg cccctgatag cgttcagctg catcgctcac taaatctgcc
     1861 tggcgaatga ccagctcgga tttatccagt gccagttgag caaaggttcg gagctgatta
     1921 ttcacttctg attttgccca aaggaaggca atccatagcg aaagaatgat gggaaagaga
     1981 actaaaaaga taatgcccgg caacgccagt aattggtgtc gtgcacgatg actcattgct
     2041 atcaatttcc tgcctgttta tacccggcca ttgcaaggtg aaaaccctgg gtaccgacgt
     2101 ctttagataa atcttacggt ataaatagca cttttgcctg atttgaaatt gatacctttc
     2161 taatcgtcac ctggatgaaa ataattcatt gatattcaaa attaatttcc tacaggaatg
     2221 cagaggcggc gggaagatat gcccttaatg ccactgaaaa accacataat ctgtacagca
     2281 ataaaagtca cggcctaata tggctaacaa acaatcaatt gtgatatagc tcacaaaatt
     2341 aatgaaacaa acagagtgtt tcatttttgt gttctgcaag ccccgcgata gccagagtgt
     2401 accacgcctc ccgtgaacaa cgccgcgctg tccagggcat cggctctttt cagaggaatt
     2461 gtttgatggc taccctcacc actggcgtgg ttcttcttcg ctggcagctt cttagtgccg
     2521 taatgatgtt tctggccagc acatcaacat ccgttttcgt cggtctgatt atgtcgggct
     2581 tgcggtgatc agcagcggtc tgggcgttgt ttccgcctgt tggttcgcaa tggggttgct
     2641 tggcatcaca atggcggaca tcaccgctat ctggcacaac atcgagtcgg tgatgataga
     2701 agagatgaat cagacaccgc cacaatggcc aatgattttg acctgatacg tagaagcctc
     2761 cataacggag gcttcttttt tatggctggc agatgcttta atcatccacc ttaaaacaat
     2821 ataacctatt gttttaatga caaatcagaa cggaatgtca tcatcaaagt ccatcggcgg
     2881 ctcgttagac ggcgctgccg gagcggactg ctgcgggcga gactgcgcgc cgccgctgaa
     2941 ctgattgcca ccctgcggct gctgaggctg cccccaaccg ccctgcggct gaccaccacc
     3001 gatattacca cctgccggag cgccaccacc ctgacgacca cccagcatct gcatggtgcc
     3061 gccaacgttc accacgactt ctgtggtgta gcgatcctga ccggattgat cggtccattt
     3121 acgggtacgc agctgacctt cgatataaac ctgagaacct ttacgcagat attcgctggc
     3181 cacttctgcc agtttgccga acagcacaac gcggtgccat tcagtctgtt ctttcatctc
     3241 gccggtcgct ttatcacgcc aggattcgga agtagccagc gtaatgttgg caactgcgcc
     3301 accatttggc atgtagcgta cttccgggtc ctgacccaga ttaccaacga gaataacctt
     3361 gtttacgcct ctgctggcca tgttcgtgtc tcctgaaaaa aatcgttctg aataagtgta
     3421 aacgcgcgat tgtaccatta ccaatagcgc ttttactatg ttgtgacctc ggttccggga
     3481 aacaaacctg gccagacatt gttacacaac actccggata atgcattcca atactgtata
     3541 ttcattcagg tcaatttgtg tcataattaa ccgtttgtga tcgccggtag caccatgcca
     3601 ccgggcaaaa aagcgtttaa tccgggaaag gtgaatggat aagatcgaag ttcggggcgc
     3661 ccgcacccat aatctcaaaa acatcaacct cgttatcccc cgcgacaagc tcattgtcgt
     3721 gaccgggctt tcgggttctg gcaaatcctc gctcgctttc gacaccttat atgccgaagg
     3781 gcagcgccgt tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt cactgatgga
     3841 aaagccggac gtcgatcata ttgaggggct ttctcctgcc atctcaattg agcagaaatc
     3901 gacgtctcat aacccgcgtt ctacggtggg gacaatcacc gaaatccacg actatttgcg
     3961 tttgttgttc gcccgcgtcg gcgaaccgcg ctgtccggac cacgacgtcc cgctggcggc
     4021 gcaaaccgtc agccagatgg tggataacgt gctgtcgcag ccggaaggca agcgtctgat
     4081 gctgctcgcg ccaatcatta aagagcgcaa aggcgaacac accaaaacgc tggagaacct
     4141 ggcaagccag ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc tttccgatcc
     4201 gccgaaactg gaactgcaaa agaaacacac tattgaagtg gtggttgatc gcttcaaggt
     4261 gcgtgacgat cttacccaac gtcttgcgga gtcgtttgaa accgcgctgg agctttccgg
     4321 tggtacagcg gtagtggcgg atatggacga cccgaaagcg gaagagctgc tgttctccgc
     4381 caacttcgcc tgcccaattt gcggctacag tatgcgtgaa ctggagccgc gactgttttc
     4441 gtttaacaac ccggcaggtg cctgcccgac ctgtgacggc cttggcgtac agcaatattt
     4501 cgatcctgac cgcgtgatcc aaaaccccga gctgtcactg gctggcggtg cgatccgtgg
     4561 ctgggatcgc cgcaacttct attatttcca gatgctgaaa tcgctggcag atcactataa
     4621 gttcgacgtc gaagcgccgt ggggcagcct gagcgcgaac gtgcataaag tggtgttgta
     4681 cggttctggc aaagaaaaca ttgaattcaa atacatgaac gatcgtggcg atacctccat
     4741 tcgtcgtcat ccgttcgaag gcgtgctgca caatatggag cgccgttata aagagacgga
     4801 atccagtgcg gtacgtgaag aattagccaa gtttatcagc aatcgcccat gcgccagctg
     4861 cgaaggaacg cgtctgcgtc gggaagcgcg ccacgtgtat gtcgagaata cgccgctgcc
     4921 tgctatctcc gacatgagca tcggtcatgc gatggaattc ttcaacaatc tcaaactcgc
     4981 aggtcagcgg gcgaagattg cagaaaaaat ccttaaagag atcggcgatc gtctgaaatt
     5041 cctcgttaac gtcggcctga attacctgac actttcccgc tcggcagaaa cactttccgg
     5101 cggtgaagcc cagcgtatcc gtctggcgag ccagattggt gcgggcctgg ttggcgttat
     5161 gtatgtactg gacgagccgt ctatcggcct gcaccagcgc gataacgagc gcctgttggg
     5221 tacgcttatc catctgcgcg atctcggtaa taccgtgatt gtggtggagc atgacgaaga
     5281 cgcaattcgc gccgctgacc atgtgatcga tatcggtcct ggtgcgggtg ttcacggcgg
     5341 tgaagtggtc gcagaaggtc cgctggaagc gattatggcg gtgcctgaat cgttgaccgg
     5401 gcagtacatg agcggtaaac gcaagattga agtgccgaag aaacgcgttc cggcgaatcc
     5461 agaaaaagtg ctgaagctga caggtgcacg cggcaacaac ctgaaggacg tgacgctcac
     5521 gctgccagtc ggtctgttta cctgcatcac aggggtttca ggttccggta aatcgacgct
     5581 gattaacgac acactgttcc cgattgccca acgccagttg aatggtgcga ccatcgccga
     5641 accggcaccg tatcgcgata ttcaggggct ggagcatttc gataaagtga tcgatatcga
     5701 ccaaagccca attggtcgta ctccacgttc taacccggcg acctataccg gcgtgtttac
     5761 acctgtgcgc gaactgtttg cgggcgtacc ggaatcccgt gcgcgtggtt atacgccagg
     5821 acgtttcagc tttaacgtcc gtggcgggcg ctgcgaagcc tgtcagggcg acggtgtgat
     5881 caaagtggag atgcacttcc tgccggatat ctacgtgccg tgcgatcagt gcaaaggtaa
     5941 acgctataac cgtgaaacgc tggaaattaa gtacaaaggc aaaaccatcc acgaagtgct
     6001 ggatatgacc atcgaagagg cgcgtgagtt ctttgatgcg gtgccagctc tggcgcgtaa
     6061 gctgcaaacg ttgatggacg ttggtctgac gtacattcgc ctggggcagt ccgcaaccac
     6121 actttctggt ggtgaagccc agcgcgtgaa gctggcgcgt gagctgtcaa aacgcggcac
     6181 cgggcagacg ctgtatattc tcgacgagcc gaccaccggt ctgcacttcg ccgatattca
     6241 gcaactcctc gacgtgctgc ataaactgcg cgatcagggc aataccattg tggtgattga
     6301 gcacaatctc gacgtgatca aaaccgctga ctggattgtc gacctgggac cagaaggcgg
     6361 cagtggcggc ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcgg agtgcgaagc
     6421 ttcgcatacg gcacgcttcc tcaagccgat gctgtaatcg ttaaggccgc tttctgagcg
     6481 gccttttcct ttcagagttg taccagcaat ttacgctttt cttccggtag taaattcact
     6541 gcctgctgat aagacgcatc caccagataa tagatttgcg aatccggcag cgaaccgtcg
     6601 agatacacgg tgctccagtg cgctttattc agatggcggc taggacgcac atcgctgtgc
     6661 tgctgacgta gcagctccgc cagctccggg ctggttttca gcgaaacagc tgggcgattt
     6721 tctacttctt tcaccatcgc aaacagtaca tcttccactt tgatctgcgt cgctttccag
     6781 tcgttatgca cgctctgttc tgcgcctggt tttgccatgc aatattgtag caactccgaa
     6841 atggtcattt tttactcccc ttgtagtgtc gcgatgatgc gacgcgatcc gccgtggatg
     6901 cgatgttccc ccagccagat gccttgccag gtgccggtct gaatacgccc tttatgtaac
     6961 ggcaatacaa gcgatgttcc cagcattgag gatttgatat gagaaggcat atcgtctgct
     7021 ccctcatagt catgctcata atttccgttg tcgggaacgg tgcggaggaa aaaacgctcc
     7081 atgtcgtggc gtacggtggg atcgcagttc tcattaagtg tcagagaggc ggaggtatgt
     7141 tgcagcaaca gatgcagtaa gccgatgtta acgcgcggca tatcagccag ctgattcaga
     7201 atttcatccg ttaccagatg aaacccacga gatttggcgc taagcgtgag cgtcttttga
     7261 taccacatgt gctgctcctt gataaaactc tcttaatcag tttgcagcaa gacagcgaaa
     7321 ggataaaggt gtgattaaaa aaacagcatt cgggagagca tcacgctctc ccgctctgtc
     7381 agtattctga attgacgatc acctcttcac caaacgcacc cgcttgtggc aagggtttgt
     7441 aggtagagtt ggaggcgcgc agaatgcgga taccacgagc gccgacatcg cgtgcggcgg
     7501 taatatcgtt atcggaatcg ccataaaaaa ttcggatatt tttatcctgc agccattgcg
     7561 attttgtatt ttgccctggt ttatcgcccg caaagattac cggattcatg ttggtggcag
     7621 gaatatgaaa attatccgcc agcgtttttg aaaccgtttc tgttttcgtc gggctacgac
     7681 cagtcacaaa gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc tggcgagcga
     7741 cctcttttgg aatgctgaat tcatcccagc cattattcat tttttcccag aacacaggat
     7801 ttttcagata atcttcgctt tctggcgaga aggttttttt gccgcgccag aaccccggac
     7861 tggaaaaaag taccgtgtca tcgatatcaa accccaccgc cattggcgga cgccctgcga
     7921 ggctattttc aatttgtgcg accgaaaccc aatgaattgg tgcctgttca gcaagcctgg
     7981 caacgttagt cccagggtta agcggtgaag gagatgaggc cagggcaaca gcggaactgt
     8041 ttagagcgaa caataagcaa acggcactga ttgcctgtgt gatcttgcgc atatttttcc
     8101 ctaaatagtc agtatgttga aactttttat tgtgagattt gttgcaaaaa ccatctgacc
     8161 ataacgccag ctgccgggga aaggaaggat tatctgcggt ttttgtgatg aaaagcagga
     8221 tgattatatt taacccagta aatacgttcc tgtctttttt tgtataaaaa atgtttatta
     8281 ctcgatgtaa ggatacatta attttacgtt tattatgatg aatcctctgc aaatgagcga
     8341 aatttgaaag aacatttatc tgctgtacga tttaatgaac ttaatgatac agcattacaa
     8401 aaacaatcga agtttataaa gatgattcct gattgaccag aaccctatcc tacaatggtg
     8461 actaatttta ttactcccga tggagatgat gatatgaata tatcatatgt gaatagcaac
     8521 aaaacgacat ctttacctgt tgagctagat gcactaaata ataaagacat ctcttatgcc
     8581 aaagattttt cttatgctaa agatttcttt ttatacatag aaacacagtt aaaaattgca
     8641 aaagattttt gtagacctgg agaagaagta tcaagttcta ttgcaagtaa agtttttcac
     8701 gcatttatcg atttagttaa caaaatcagg ggtaagaaag actttatgta tatttgcacg
     8761 ctttgctgtt ttgccgagga ggttaaaggt gattattctc attacaggac ctttttattt
     8821 gatattggta atcaatataa ggttaaactt acacaaagcg gaaaaaaaga gttctcttta
     8881 acattagaat ttaacgatac tataattgaa tctcagaaag tcacaggcaa taaggcaaag
     8941 tatattcttg aggatataga aatattctat cgtaacaaac ccgatactta ttattaaatt
     9001 taataatggt tagatgtgca gaaattatta ctccacacga aaaaataggg aaaggaattc
     9061 cagataattg ggataaactt aacaataaaa gaactgacaa cgttttatta tctctcacgt
     9121 agaacaacgg catcaaaaaa atcatctaca aagaaatagt gaaagcgacg acagtttaat
     9181 ttcactgcag gctgggtaac tccagcctgc tttcctgcat tacatcaccg cagcaaacgc
     9241 ctttgccaca cgttgtacat ttgccgtatt taacccggcg acacacatgc gaccgctggc
     9301 gatgagatag acaccaaatt cttcacgtag tcggtcaacc tgagcggcac ttaaaccggt
     9361 ataactgaac atgccgcgct gattaagcag ataatcgaaa ttgcgttctg gcatctctgt
     9421 gcttaatacc ttcaccaatt cctgacgcat tgccagaatg cgagtacgca tctcttctac
     9481 ttccgccagc cagctggctt tcaatgcctc gtcattcagc actgcagcca ccacctgcgc
     9541 accaaaattc ggcgggctgg agtagttgcg gcgaactgtt gctttcaatt gccccagtac
     9601 gcggccagcg gcttcggcat cttcacacag aacagaaagt ccgccgacgc gctcgccgta
     9661 aagggagaaa attttcgaga acgaattgct caccagagcg ggtaatccag cgctggcaat
     9721 ggcgcgaata gcgtaggcat cctcttccat accggcacta aatccttgat aggcaatatc
     9781 gaggaatggg ataagctcgc gggctttgag aatttcaatc accgcatccc actgatcatt
     9841 agtgagatcg gcacccgttg ggttgtggca acatggatgc agcaacacaa tactgcgggc
     9901 aggtaatgtt ttcagcgtcg ccaacaggtc attaaagcgc acgctgttag tcgcttcgtc
     9961 ataccagggg taagtactca cttcgaatcc agccccggcg aatattgcta cgtggttttc
    10021 ccaggtagga tcgctgaccc agacgcctga ttccgggaag tagcgtttca ggaaatccgc
    10081 gcccactttc aatgcccctg agccgccaag ggtttgaatg gttgctacgc gctgttgttg
    10141 cagtaccgga tggtccgcac caaacagcag cggcgcaatg gcatggcgat agctgttaag
    10201 cccttccatc ggtaaataaa gcgaagcgcc atggggctgc gcattcaggc gcgcttccgc
    10261 ctccgccacg gctttcagtt gtggaataat tccgtcttcg ttgtagtaca gaccgatact
    10321 taaattcact ttgtcgctgc gagggtcttc tttaaaacgc tccataagcg taagaatcgg
    10381 gtcgccagcg taggcgtcaa ctttttgaaa cacgcgatgg ttctccaggt ttacgggcag
    10441 gtggttaaaa cacaataaac cggaagaagg cgaagatcga gtggatgttc aggagcgaac
    10501 ggcaattagc aacagagtga gactcatgac aaacgaacat ccgccagagg cacgaccttt
    10561 ataagagcat gaaagaacat caacttattg aatttttagg attttattgg ccggataagg
    10621 cattcacgcc gcatccggca cagacaatca aatattacag aacgattaat ccacgtattt
    10681 catcgcgacc cttgaagtca ggcgcgtaat aagttcgtaa gcgctcactt ttgtcatttc
    10741 agcgatacgt tctacgggca aaccttcgcc ccataaaatg accggatccc cggctttgtc
    10801 ctgcgcctgt ggacctaagt ctacgcagat catatccatc gccacgcgtc cgacaatcgg
    10861 tacttcgcga ccgttcacca gcactggcgt accggatgga gcggcgcgcg gataaccatc
    10921 gccatagccc atcgcgacta cgccaagacg ggtatcacgt tcgcttaccc aggttccacc
    10981 ataaccaaca ggctctccgg ctttatgctc acgcacggca atcaggctgg aggttagtga
    11041 catcactggc tgacagccaa aatcggcacc gatggagcga tcttccagcg gcgagacgcc
    11101 ataaagaatg atgcccgggc gcacccagtc aaaatgcgat tgtggccaca gcagaatgcc
    11161 acccgacgcg gcaatggaac gttgaccagg tttgccttcg caaaaggtat taaagatagc
    11221 gagttgtttc tcggttgcgc cacattttgg ttcatccgcg cgcgcaaaat ggctgacgat
    11281 attcaccggc tgacgaacgt ttttgcactg tgtcaggcga tgataaaacg cctcagcctg
    11341 ttccggcctt acgcccagac ggtgcatacc ggtatcgagt ttcatccaga cggtaaccgg
    11401 ctcgtccagg ctagcctctt ccagcgcagc cagctgttct tcgttatgca cggcggtatg
    11461 aaaatgttgc gcagaaatcg tcggcagatc tctggcatca aaaaagcctt cgagtaacag
    11521 tacaggtttg gtgattcccc ccgcacgcag tcgcagagct tcttcgagac gggctacgcc
    11581 aaaggcgtca gcatcgggga gcgttcgcgc ggtctcaaga agaccgtgac cataagcgtt
    11641 cgctttcacc accgcaacca ttttactggc aggggccagt tcacgaagac gttgcaggtt
    11701 gtgtcgcaga gcgcggcggt taatcacaac agttgccgct tgcatttgtg ttccttgata
    11761 agtgtttgct ttaattacct aattcataaa ataattatta ttcgtcgtcg tactgcggcc
    11821 ccgcatagtt gtcgaagcgc gaccattgac cgttaaaggt caggcgtacc gtcccgattg
    11881 ggccgttacg ttgtttaccg ataataattt ccgcgatgcc ttttaaatca ctgttttcgt
    11941 gatacacctc atcacgatag ataaacataa tcaagtccgc atcctgctcg atagagccag
    12001 attcacgcag gtcggagttg accgggcgtt tgtcggcacg ttgttccaga gaacggttca
    12061 actgggacag cgccaccacc ggcacgttca gttctttcgc cagtgctttc agcgagcgag
    12121 agatttctgc aatttccagc gtacggttat cggaaagcgc cggtacgcgc atcagttgca
    12181 ggtagtcgat catgataagc ccgatgccgc cgtgttcacg ggcaatacgg cgtgcgcggg
    12241 aacgcacttc cgttggcgtc aggccggagg agtcatcgat atagatattg cgtttttcga
    12301 gcaaaatacc catggtgccg gaaatgcgcg cccagtcttc atcatcgagc tgcccggtac
    12361 ggattttagt ctggtcaacg cgcgacagcg acgccagaga acgcatcata atctgttctg
    12421 aaggcatctc cagcgagaag ataagtactg gtttatcctg caacatcgcc gcgttttcga
    12481 cgaggttcat cgcaaatgtt gttttaccca tcgacggacg cgcggcgacg atgatcaaat
    12541 ccgacggctg caagccagcg gtttttttgt tgagatcgtc ataaccggtg tttactccgg
    12601 taacgccatc gtgtggctgc tgaaacaact gctcaatacg cgccacggtt gcgtcgagca
    12661 catcggcgat gttcttcggc ccttcgtctt tgtttgcacg actttcggca attttaaaga
    12721 cgcgggattc agcaaggtcc agcagatctt cgctggtacg cccctgcgga tcaaaaccgg
    12781 cttcagcaat ctcattcgca accgagatca tctcacggac aacggcacgt tcacgcacga
    12841 tgtcagcata agcactgatg ttcgccgcac ttggcgtatt ttttgacagc tctgccagat
    12901 aagcaaaacc accgacgcta tcgagttgcc cctggcgttc cagcgattcc gcaagggtaa
    12961 tcagatcgat aggactaccg ctttcctgca aacgcgccat ttcagtaaag atatggcggt
    13021 gtgggcgggt gtaaaaatcg tctgccacca cacgctcggc tacatcgtcc cagcgttcgt
    13081 tatccagcat taaaccgccc aacaccgact gctccgcttc gatcgagtgc ggaggcactt
    13141 tcagcccggc aacttgtgga tcgcgttcgc ggggttcagc ctgctgtttg ttgaagggtt
    13201 tatttcctgc catagtgaat ggagttaccg agataaagaa tgggtcgaaa ctttaccata
    13261 tgaagcagac cctgacgata cgttctggag gacacatggc aacacgaatt gaatttcaca
    13321 agcacggtgg cccggaagta cttcaagccg tagagttcac tcctgccgat ccggcagaga
    13381 atgaaatcca ggtcgaaaat aaagccatcg gcatcaattt tatcgacacg tatatccgca
    13441 gcggccttta cccgccgcca tcactaccca gcggattagg taccgaagcg gcaggcatcg
    13501 tgagtaaagt cggcagtggt gtaaagcata ttaaggcagg cgatcgtgta gtctatgcgc
    13561 agtcggcgtt aggcgcttac agctctgtgc ataacattaa tgcggataaa gcggcgattc
    13621 tgcctgcggc aatttctttt gagcaagctg cggcatcctt cctgaaaggc ttaacggttt
    13681 attatctgct gcgcaaaacc tatgaaatta aacccgatga acagttcctg ttccacgcag
    13741 cggctggcgg cgttggctta attgcctgcc agtgggcaaa agccctgggc gcgaaactta
    13801 tcggcaccgt aggaaccgcg caaaaagcgc agagcgcgct aaaagcgggc gcgtggcagg
    13861 ttattaacta tcgtgaagag gatctggtcg agcggttaaa agagatcacc ggcggtaaga
    13921 aagtgcgcgt ggtgtacgat tccgtgggca gagacacctg ggaacggtcg ctggattgcc
    13981 tgcaacgccg cggcttaatg gtcagttttg gcaactcatc aggtgcggtt accggtgtga
    14041 acttaggcat tctcaatcaa aaaggctcgt tgtatgtgac acgcccttcc ctgcaaggct
    14101 atatcaccac gcgggaggaa ttaaccgagg ccagtaatga actgttctct ttgattgcca
    14161 gcggtgtgat taaggtcgat gtcgccgagc agcagaaata tccgctgaag gatgcgcagc
    14221 gtgcgcatga gattctggaa agccgggcga cgcaaggttc cagcctgttg attccataaa
    14281 agaaataggg cttccacctg ggaagccctt tctttttata gttcggctgt atgtagggta
    14341 cagcacgatg aatctgttag aggcgcaata gtgacagatt tgattatcaa ttcctatttt
    14401 gttctaagga taaaacctta ggttgtgatc atccgcacaa tcccttagta acgccagcgg
    14461 tcataacgct gatatttcgg cacttttggt gctttaatcg ccttaataac ccacaccacc
    14521 gcaatcgcca gtagtaacca cggcagcaac ttaatcatca atgccagcat accgccgagg
    14581 aacataatgg ccgtcgccac aaccagcgcg gcgataatgc ccagcaacga aacgccggtg
    14641 accatcagca tgacaaaaaa gccaatcaca aaaagtagtt ccagcatgat gctctcccaa
    14701 atatgaaatc tcttgctggc attacaagaa tcatgccaaa aataatctat tgatttaaca
    14761 gcaaaacgcc ccgcgacggt gcgcagggcg tggtgaattt gactactttt tggtgaaaag
    14821 ttaacgctta tccgccacca gtttgagcgc gtgttccagc acattaatgt ctgcacccgc
    14881 tttatgggca ttttcactta aataacgccg ccactgccgc gcgccaggaa taccctggaa
    14941 caagcccaac atatgccggg taatatggcc gagatacgtc ccctggctga gttcacgctc
    15001 aatgtacgga tacatggcgc gcactaccgc caccggatcg gcatcggtat ccgaggaacc
    15061 aaagatctcc cggtctaccg ccgccagaat acccggattc tgatacgcct cgcgcccgac
    15121 catcacgcca tccatatgtt gcaggtgtgc tttggcctct tccagcgact tgataccacc
    15181 gttaatcgac attgtcagat gcggaaagtc acgcttcagt tgatacacac gcggataatc
    15241 gagcggcggg atttcacggt tttctttcgg gcttaacccc gaaagccagg ctttacgtgc
    15301 gtggatgata aacatctcac actcgccttt gccggaaacg gtgttgatga aatcgcagag
    15361 aaattcatag ctgtcctggt catcgatgcc aatacgcgtt ttcaccgtca ctggaatcga
    15421 caccacatcg cgcatcgctt tcacgcagtc ggcaaccagc tgcgcattac ccatcagaca
    15481 cgcaccaaac atgccgttct gcacccggtc agacgggcag ccgacattca ggttgatctc
    15541 atcatatcca cgcgcttctg ccagcttcgc acactgtgcc agcgccgccg gatcgctacc
    15601 gccgagttgc aacgctaccg gatgttcttc ttcactgtac gccaggtaat cacctttacc
    15661 gtgaataatc gcccctgtgg tcaccatttc ggtatacagc aacgtattgc gggaaagcag
    15721 acgcaagaaa tagcggcaat gtctgtccgt ccagtcgagc attggtgcaa cgctaaaccg
    15781 accactccag tgaacgtcag ttttttcagg cattgcgctg gtttggttga ttttttgcat
    15841 ttcagaatta ccgtgcattt tcaaatgtag agattatttt atcgatatat catggggtta
    15901 tgttattcag catcactatt caggaggctc aatagcgggg tactatacca taacaacagg
    15961 aagcgcctgt ctcattgcaa aagaaaattg agatcctctc aaggcatgaa gctctcacga
    16021 agtgatggaa ataatcttat tagccgttaa ctttgttaag gccaatgata aacaatccag
    16081 gttcgacgat aaataaaaaa tcacacatta aactctggtg atatatctcc ctgctaatag
    16141 cattgataga gaaaaaagaa cccaataaat attgggtcct tttatataat gccttccata
    16201 ctatcgaaga acttcacata ttatttctcc gatttaaccc cgaacaaatc ataaattaat
    16261 ttagaagtgt ctgtaagtat tttaatctct tcctttgagg ttgggtcaaa cgacttcgcg
    16321 aagctaatta attgtggtgc agcatctcgc attttgctta aaatatcagg atcgagcgtt
    16381 ccttcattca ccagatgtga catcttatcc agatagccat caaaattcat ttctccccca
    16441 tcagccagac gcttcatctc tcccaggtac ttcttcatat cacgttgaga taatttttca
    16501 aactgagctt tcaggtagct ctcattattt tcattaatat acactgtttc tgataaatct
    16561 gcgtaggcac tagaatatga aagtgaaatt aacagtgaag caaataattt taagctgttg
    16621 tcatcatgtt cctggcaggc attaacaaac gttaaaaaac cagaacctat tgcttgaaaa
    16681 tgtatattcg ctaaaggttc attatcctta gattcctcat aaaatagctg aaccgtggag
    16741 ggaaggccat tcttagtttc tacatcaaaa gtacattgtt caatgggagt cgcatcttta
    16801 ttatccccag gattacatat actattaatc gcgaaatgaa aaatagcccg gtcaacaact
    16861 gatgcaataa agtcacgatc gtttaaatcc tcatcagtgc aatcgtcaat atacttattg
    16921 acccacattt tgagatcatt tatttgctca attaaaaaca cattttttta cgtgcatgcc
    16981 ataaattatg cgtgattcgt tgaataaaca aatctatttt tgcaataatt atatcccgat
    17041 gacgcagcgc tgcataatca atagatttac catatatatt atttaattta cctttgattg
    17101 aacctctatt atctaattta tctttgattg catcttcatt atcgaattga tttgaattga
    17161 gtgttttatt aaagtatcca gttgcaccgg gtattatctt caacatgatt catctacctt
    17221 ttttgtcgta ccgtgtcctc cttataattc ttttgccatc tggtcactat caaaaatcgc
    17281 tcataatttc tgaactacag gtatgcgttt aaacccagtt aaataaatag cgagttaact
    17341 tgaagcaatt tatttaaaaa tgaattatta attattatcg tgttgataat ggtacgacaa
    17401 ggcaacataa caccttacct acaaaacaga aaatcaacat tacggcaaca ataagggttc
    17461 tcggtgtttt tcctggaaca tggtaaagta aggacattct taacccccac tttgaggtgc
    17521 ccgatggaaa agaccacaac gcaggagtta ttagcgcagg ctgaaaaaat ctgcgcgcag
    17581 cgtaatgtgc gcctgacccc acagcgcctg gaagtgttgc gcctgatgag tctgcaggat
    17641 ggcgctatca gcgcttatga tctgcttgat ttactgcgcg aagctgaacc gcaagccaag
    17701 ccgccaacgg tttatcgcgc gctggatttt ctgcttgagc aaggttttgt gcataaggtg
    17761 gaatccacca acagttatgt gctctgtcat ctgttcgacc agcccacgca tacgtcagcc
    17821 atgtttatat gcgatcgctg cggcgcggtg aaagaagagt gtgcagaagg cgtggaagac
    17881 attatgcata cgctggcggc aaaaatgggg tttgccctgc ggcataatgt gattgaagca
    17941 catgggctct gtgcggcatg cgtggaagtg gaagcgtgtc gtcatcctga acagtgccag
    18001 catgatcact ctgtgcaggt gaaaaagaaa ccgcgttaag agggcgtaca tccttgtaca
    18061 cgtcgggcag gagggattaa ttaccagcga tattcattgc gggtttccca atccacgacc
    18121 tctttttctg cctgatcttt ctgataacca taccgttcct ggattttacc gaccagttga
    18181 tcacgtttac cttcaatgat cgtcatatca tcatcggtca gtttgcccca ttgctctttc
    18241 actttacctt taaactgttt ccagttaccg ccggcttcat ctttattcat aatcaagacc
    18301 tcatcgttag gttgtgaatg agagtacgtt cacttttctt ctgaacgtga gattaagtgt
    18361 agtcagcaat ttggcttagg ataatttatt cagaattttt aaccgtcacg ttgcggcaaa
    18421 ccaggtaccg ttacgccagt gacgccgcca gatagccgcc agagaaagcc cgcgcaacgc
    18481 cagaaagacg gttagtgcca gccacaaacc atgattaccc agccacggca gcgtaaggag
    18541 cgtcagcgca aaacctgcgg cggccaccgc catactgtta cgcatttcgg cggcacgcgt
    18601 tgcgcctata aacatgccgt ccagcagata acaccagacg ccaaccaacg gcaaaatcac
    18661 ctgccagata agatagcggt cagccagctg ctgaatctgg gttaacgacg tcagcaacgc
    18721 aatgatgtgt tcccctgcca gcaaataaac caccgaaaat agtaacgcta cgatccccga
    18781 ctggcggcac gctgcccgcc agacatccag caactggcta ccgtcgcgcg caccgtatgc
    18841 ctgaccggaa tgtgcttcaa ccgcgtaggc aaaaccatcc agcgcatagg cggtaaaggt
    18901 gagtagcgtc atcagaaccg cgtttacagc gataatgtca ctccccagtc gcgcgccaag
    18961 tacggtgatc gcgccgaaac agagttgcaa caacagcgaa cgcagcatga tatcgcggtt
    19021 aagcgccagc aagcgacgga agtttcctcg ccaggcagtt ttcgacattt cgccggaaat
    19081 tccgcgtagt ttgaggattt tacgcaccat tagcagacca atcagcaatg ttgcatattc
    19141 cgcaataacc gtcgccagag ccgcgccctg cacgttcata tgcagcccca tcactagcca
    19201 gacatccagc acaatgttga ggatattgcc gaccactaac aaaattactg gcgcacgggc
    19261 atattgcacg ccgagtaacc aaccgagtaa taccagattc gccagcgacg ccggtgcgct
    19321 taaccagcgg atttcaagaa agcgccgcgc ctgttccagg actgcttcac taccgccaac
    19381 aatatgcaga gccagatcga taatcggtgt acgcagcagc gcaattaacg ccccagcccc
    19441 caacgccaac agcaacggtt gcaccagcgc acgggctaat gcctgaggat ttttggcacc
    19501 ataagcctgc gcagtcagcc cggtggtgct catgcgtaaa aacagcaaca gcataaagag
    19561 aaagctggtc gccgttgcac caaccgccac gccgcccaaa taaaccgggc tatcaagatg
    19621 accaattacc gccgtatcga ccagtcccag caacggaacg gtgatattgg agaaaatcat
    19681 gggtaaggcg agatgccaga gtgctttatc agatgaagtg aggaatgcca tgcagacaag
    19741 cctgatgaag agagatgaaa aacaaaccgc gacgcctggc ggcatcgcgg tctcagagat
    19801 atgttacagc cagtcgccgt tgcgaataac cccaaccgcc agcccttcaa tggtgaagct
    19861 ctgctgacga aggtcaacga caattggttt aaactcgcta ttttctggca acagttcgac
    19921 tttattaccc tgttttttca ggcgcttaac ggtaacttcg tcatcaatac gtgcgacaac
    19981 gacctgaccg ttacgtacat cctgagtttt atgcactgcc agcaagtcac catccataat
    20041 gccgatatct ttcatcgaca tcccgctgac gcgcagcagg aaatcagcat tcggcttgaa
    20101 caaggaagga tcgacctgat aatgaccttc aatatgctgc tgcgccagaa gcggttcacc
    20161 ggcagccaca cgacctacca gcggcaaccc ttcttcctct tcctgcaaca gacgaatccc
    20221 gcgtgatgcg ccggaaacaa tttcaataac gcctttgcgt gccagcgcct tcagatgttc
    20281 ttcagccgcg tttggggaac ggaaccccaa acgctgcgcg atttccgcac gcgtcggcgg
    20341 catacctgtc tggctgatgt gatcacggat gagatcaaac acctcttgtt gcctggccgt
    20401 taacgctttc attccgcccc ctgggtgtat atacagttat gctgtgagta tatacagcaa
    20461 aaggcgattt tggaaccata aactgcacaa taaaccagag atttatcgaa atctggaagg
    20521 gttatccaaa atgcgaccat aacaggatgc accaggttat cacggcgacg ataatcgcaa
    20581 tcagcaccgc ggcggatccc atatctttag cgcgtccgga aagctcatgg tattcagagc
    20641 caattcggtc aaccactgct tcgatggcgc tattgagaat ttccacaatc atcaccagca
    20701 tcacggagct gataagcagc acgcgggtaa ttgcgtccac atccagccag caggcgatga
    20761 ccaccgccaa caataccgct acgccttcct gacggaatgc cgcttcgttg atccatgcag
    20821 cgcgtaaacc tttccaggaa tagccagcag ctttgataat tcgggtgaat ccagtggtat
    20881 tattggccat tgaaagaacc tttttacatt atgagcgtca atatcagtgt accgttaaat
    20941 ccgatgattc gggtttgcgt aacgccgcgc gaaacatgag cggataccac agaatttccc
    21001 atgactttct gctatccttg ccgcgcattt gcattattaa ccagaggctt tacatcgttt
    21061 atgtccggct ggccacgaat ttactacaaa ttactgaatt taccattaag catcctggta
    21121 aaaagcaagt ctattccggc agatcctgcc ccggaactgg ggctggatac ctctcgtcca
    21181 attatgtacg ttttaccgta caactcgaaa gcagatttgc tgacgttacg cgcccagtgt
    21241 ctggctcatg acttgcctga cccgttagag ccgctagaaa tcgacggcac gctactgccg
    21301 cgctatgtgt tcattcacgg cgggccgcgt gtattcacct attacacgcc gaaagaagag
    21361 tctattaagc tgttccacga ctatctcgat ttgcaccgta gcaacccaaa tctggatgtg
    21421 cagatggtgc cagtatcggt gatgtttggt cgcgcgccgg ggcgtgaaaa aggcgaagta
    21481 aacccgccgc tgcgtatgct taacggcgta cagaaatttt tcgccgtact gtggctcggt
    21541 cgcgacagtt ttgtgcgttt ctcgccgtca gtttcgctgc gccgtatggc ggatgaacac
    21601 ggcacggata aaactatcgc acagaaactg gcgcgcgtgg cgcgtatgca ctttgcccgt
    21661 caacgtctgg ctgccgtagg cccacgtctg cctgctcgtc aggatctgtt taataagctg
    21721 ctcgcctccc gcgccattgc caaagcggta gaagatgaag cgcgcagcaa aaaaatctcc
    21781 catgaaaaag cgcagcagaa cgcgattgcg ctgatggaag agattgcggc gaatttctct
    21841 tacgagatga tccgcctgac tgaccgcatt ctgggcttca cctggaaccg actttatcag
    21901 ggcatcaacg tccataacgc cgagcgcgtt cgccagctgg cccacgacgg tcatgagctg
    21961 gtgtatgtgc cttgccaccg cagtcacatg gactacctgc tactttctta cgtgctgtac
    22021 caccaggggc tggtgccgcc gcatatcgcc gccgggatca acctgaactt ctggccggcc
    22081 gggccgattt tccgccgtct gggggcgttc tttattcgcc gtacttttaa aggcaataaa
    22141 ctttattcca ccgttttccg tgagtatctc ggcgaactgt tcagccgtgg ttattccgtc
    22201 gagtacttcg tggaaggcgg tcgttcccgc acggggcgtt tgctggatcc gaaaaccggt
    22261 acgctgtcga tgaccattca ggcgatgctg cgtggcggca cgcgtccgat tacgctgatt
    22321 ccgatctata tcggttatga gcacgtcatg gaagtgggta cttacgccaa agaactgcgc
    22381 ggcgcgacga aagagaaaga gagcctgccg cagatgctgc gcggtttaag caagctgcgt
    22441 aatctcggtc agggttacgt caacttcggt gaaccaatgc cgttgatgac ctaccttaac
    22501 cagcacgtac cagactggcg tgaatctatc gatcccatcg aagcggtgcg tccggcctgg
    22561 ttaacgccga cggtcaataa tattgctgcc gatctgatgg tacgcattaa caacgcaggc
    22621 gcggcaaacg ccatgaacct gtgttgtact gcgcttctgg catcacgtca gcgctcactc
    22681 acccgcgagc agttaaccga gcaactcaac tgctacctgg atctgatgcg caacgtacct
    22741 tactccacgg actctaccgt tccttcagcc agcgccagcg agcttatcga tcacgcgctg
    22801 caaatgaaca agtttgaagt cgagaaagac actatcggcg acatcatcat tctgccgcgc
    22861 gagcaagcgg tgctgatgac ctactatcgc aacaacattg cgcatatgtt ggtgctgcct
    22921 tcgctgatgg cggcaattgt cacccagcat cgccacatct cccgcgacgt attgatggag
    22981 cacgtcaatg tgctttaccc aatgctgaaa gcggagctgt tcctgcgctg ggatcgcgac
    23041 gagttgccgg acgttattga tgcgctggca aatgagatgc aacgtcaggg gctgattacc
    23101 ctgcaagatg atgagttgca tatcaacccg gcgcattctc gccccctaca gctgctggcc
    23161 gcaggcgcgc gcgaaacgct gcaacgttat gccatcacct tctggttgtt gagtgccaac
    23221 ccgtcgatca accgcggtac gctggagaaa gagagccgca ccgttgcgca acgtctctcc
    23281 gtgctgcacg gcatcaacgc gccggagttc ttcgacaagg cggtgttcag ttctctggtg
    23341 ctgacgctgc gtgatgaagg gtatatcagc gatagcggcg acgccgaacc ggcagaaacg
    23401 atgaaggttt atcagttgct ggcggagttg attacatcag acgtgcgttt gacgattgag
    23461 agtgcgacgc agggcgaagg gtaatcagag agaattgccg gatgcggcga aaacgcctta
    23521 tccggccttc cataatctgc aaattcaata aattgcgatg tgttgtgtag gcctgagaag
    23581 cgcagcgcat caggcaaccc agaagaaagc cggatgatca tccggctttt ttacatcatc
    23641 agaaatgcca gtaactcatt gccagtccta aaaacagtac cagaccaaca tagttattat
    23701 tcataaatgc tttaaagcag gcttcacgct cgcggttggc aatcagtttt tgttgataaa
    23761 caaacagcgc gccagccacc agaattgacc agtaatatcc ccagcctaag ccatttaact
    23821 caccgatgat cgccatcagt gccagtacgc caatctgcaa aataccaata atcaatttat
    23881 cgtattggcc gaacaggatt gcggtggatt taataccaat cttcacatca tcgtcacggt
    23941 caaccattgc atactgcgtg tcgtaagcca ccgcccagag aatattggcg aggaacatta
    24001 accagcaact caatggcacc gactcactca cagcggcaaa agccattgga atcgaccagc
    24061 caaacgccgc gcccagcacc acttgcggga gatgggtata ccgcttcata aacgggtaca
    24121 cccacgccaa cgccagagcg gcaatcgaca acagaatggt catcgtattc agcgtcagca
    24181 ccagtaaaaa tgaaatcaat accagcacta caaacagcgc gcgcgcctct ttctccgtta
    24241 ccgcgccgct gggaagcggt cggttcgccg tgcgcttaac atgaccatca aacttgcggt
    24301 cagcataatc attcaccaca catccggcag cgcgcatcag ccagacaccc gcgacaaaca
    24361 ccgccaggat ccagagctgg ggaacgcccg gtgtcgccac ccacaacgcc cataatgttg
    24421 gccagagcag cagtaacgcg ccaattggct tatccgtacg cattaagcga tgaaacgcca
    24481 gcagcttatt ctgcgtcaga ctccactcca tatttttttc ctcttagtac aacggtgacg
    24541 ccggtaaaaa cagttctgtt agcaacagcg gtttaccgct taatcgcagg cgggaacgtc
    24601 gcccccacag tccggcatca cggcctatct caataaagtc ccgggttaat gtcgatgatg
    24661 tgaacagata gcgtcctagc ggcgttttac ccaatttttg taacgccagc tccggcccgc
    24721 ttaacgttga cacaggaacg acggtacgac cggcaagcca cggttcacca tcggcacata
    24781 acaaaatttc acgtaaccag taacgagact ctttcggcag cagcggcagt tcttcgggga
    24841 tttcattctg ctcgacaaac ccttcgcgga tcatcgtcac gctcaccgtt tttccctgct
    24901 gttcaaaacg ttttgtcatg gaatcttcca gcaacagcca gtcgagcagt tgcgaatcca
    24961 gggcagggat ctctttaaaa tagcgcagcg cacgcagttg cgttaacgcg gggtgtgaca
    25021 tgccgaactc tccgttacat aacgtaaagg cattgtatcg cagaaaagtg tttacgggag
    25081 atgtaaagca aattaagtga tctaatgcgc aacaaaaacg caacaatcgc atgaagtggt
    25141 aaaacgctgt tgaaccttac gctttgccga tgcccgtgct ggcgagggca accgtagggg
    25201 catcaccgcc catgatgcca ggaacggtga tgttttcctt tacttaaata cctatattct
    25261 ttatacgagt agctcgccaa ctcgcagtaa aaattgggaa aagcgcatat ttaccatgag
    25321 atttggagct aaaaagtgtt tcctgagtaa gattaatagg caacatctta tgtctggatt
    25381 tatagataca cgtggaattc caattggtat cttttgtagg attcaacata t
//
LOCUS       DAETQN010000057        25129 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.057, whole genome shotgun sequence.
ACCESSION   DAETQN010000057 DAETQN010000000
VERSION     DAETQN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25129
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.057"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(166..576)
                     /locus_tag="K0W68_002309"
     CDS             complement(166..576)
                     /locus_tag="K0W68_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HBI3073855.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(634..867)
                     /locus_tag="K0W68_002310"
     CDS             complement(634..867)
                     /locus_tag="K0W68_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HBI3073856.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1256..1801
                     /locus_tag="K0W68_002311"
     CDS             1256..1801
                     /locus_tag="K0W68_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HBI3073857.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1776..3701
                     /locus_tag="K0W68_002312"
     CDS             1776..3701
                     /locus_tag="K0W68_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBI3073858.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3698..3904
                     /locus_tag="K0W68_002313"
     CDS             3698..3904
                     /locus_tag="K0W68_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HBI3073859.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3901..5502
                     /locus_tag="K0W68_002314"
     CDS             3901..5502
                     /locus_tag="K0W68_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBI3073860.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5483..6802
                     /locus_tag="K0W68_002315"
     CDS             5483..6802
                     /locus_tag="K0W68_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBI3073861.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6812..7144
                     /locus_tag="K0W68_002316"
     CDS             6812..7144
                     /locus_tag="K0W68_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBI3073862.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            7200..8225
                     /locus_tag="K0W68_002317"
     CDS             7200..8225
                     /locus_tag="K0W68_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBI3073863.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8267..8665
                     /locus_tag="K0W68_002318"
     CDS             8267..8665
                     /locus_tag="K0W68_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBI3073864.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8677..9030
                     /locus_tag="K0W68_002319"
     CDS             8677..9030
                     /locus_tag="K0W68_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HBI3073865.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9042..9620
                     /locus_tag="K0W68_002320"
     CDS             9042..9620
                     /locus_tag="K0W68_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3073866.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9617..10012
                     /locus_tag="K0W68_002321"
     CDS             9617..10012
                     /locus_tag="K0W68_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3073867.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            10020..10760
                     /locus_tag="K0W68_002322"
     CDS             10020..10760
                     /locus_tag="K0W68_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3073868.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10776..11198
                     /locus_tag="K0W68_002323"
     CDS             10776..11198
                     /locus_tag="K0W68_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBI3073869.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            11180..11614
                     /locus_tag="K0W68_002324"
     CDS             11180..11614
                     /locus_tag="K0W68_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBI3073870.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11607..14168
                     /locus_tag="K0W68_002325"
     CDS             11607..14168
                     /locus_tag="K0W68_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBI3073871.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14165..14494
                     /locus_tag="K0W68_002326"
     CDS             14165..14494
                     /locus_tag="K0W68_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3073872.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14494..15192
                     /locus_tag="K0W68_002327"
     CDS             14494..15192
                     /locus_tag="K0W68_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBI3073873.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15198..15941
                     /locus_tag="K0W68_002328"
     CDS             15198..15941
                     /locus_tag="K0W68_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3073874.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15878..16510
                     /locus_tag="K0W68_002329"
     CDS             15878..16510
                     /locus_tag="K0W68_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBI3073875.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRAMATQLPAFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVMPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16571..20068
                     /locus_tag="K0W68_002330"
     CDS             16571..20068
                     /locus_tag="K0W68_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBI3073876.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            20139..20655
                     /locus_tag="K0W68_002331"
                     /pseudo
     CDS             20139..20655
                     /locus_tag="K0W68_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20720..22124
                     /locus_tag="K0W68_002332"
                     /pseudo
     CDS             20720..22124
                     /locus_tag="K0W68_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            22073..22225
                     /locus_tag="K0W68_002333"
                     /pseudo
     CDS             22073..22225
                     /locus_tag="K0W68_002333"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            22202..22381
                     /locus_tag="K0W68_002334"
                     /pseudo
     CDS             22202..22381
                     /locus_tag="K0W68_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            23096..23803
                     /locus_tag="K0W68_002335"
                     /pseudo
     CDS             23096..23803
                     /locus_tag="K0W68_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            23803..24384
                     /locus_tag="K0W68_002336"
     CDS             23803..24384
                     /locus_tag="K0W68_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBI3073877.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT         6040 a         6197 c         7555 g         5337 t
ORIGIN      
        1 gtcgccgtat tcccgtatta acagagaccg tagcccgacg gggaactcct tctgcgcgag
       61 tgtgcgggaa taatcaaaaa cgatgcacac cggggttacc gggtacacat atttcatcat
      121 gccagcgagt ccggttctgg cacggaagaa accggacgtt atgatttagt gcggaaatat
      181 ttgtgtagtg ttctgtatgt tctcagtaaa gagtaatgaa ttatcaaagg tatagtaata
      241 ccttttgttt tcgtggatat ttgtaatcca tctgaaaacc cctgctgtag caagattttt
      301 cctgtattcg taaaatgata actctcctga tttgaatcct tttaaggtgg cttctataag
      361 gcatttattt tttgaaaatc ttacatttac aaccttaccc tgtcctttta ttaaaaccgt
      421 attatcgttt tcaagaacaa gatgaatatt ctctgtggct aaatagtaaa tgtaatgtga
      481 gacattgtga cgttttagtt cagaataaaa ccagtgatag tttaaattat ttcgcacttt
      541 atcgaatatt tgtttaaaaa tggcaacctg agccattgta gtaccttcca tgtgatatga
      601 gggggcgtag tctgcacgat tatctaaatt gcttcaatct ggtctaacct gttttctgag
      661 caattcagta atgtcactct tttctttgtt tgcttcaggc gaaactcttt tttctgagca
      721 cagtctccgg cggcaggctt caatgaccca ggctgagaaa ttcccggacc ctttttgaac
      781 aagagcgatg ttaatttgtt caatcatttg gttaggaaag cggatgttgc gggttgttgt
      841 tctgcgggtt ctgttcttcg ttgacatgag gttgccctgt attcagtgtc gctgatttgt
      901 attgtctgaa gttgttttta cgttaagttg atgcagatca attaatacga tacctgcgtc
      961 ataattgatt atttgacgtg gtttgatggc gtagatgcac gttgtgacat gtagatgata
     1021 attattatca ttttgcgggt cctttccggc gatccgacag gttacggggc ggaaggcgcg
     1081 cgggttttcg ctatttatga caattttccg gtttaaggcg tttccgttct tcttcgccgt
     1141 aacctaatgt ttttatttaa aacaccccct gaaaagaaag gaaacgacag gtgctgaaaa
     1201 cgggcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat gaacaatgga
     1261 agtcaacaaa aagcagctgg ctgacatttt cggtgcgagt atccgtacca ttcagaactg
     1321 gcaggaacag ggaatgcccg ttctgcgagg cggtggcaag ggtaatgagg tgctttatga
     1381 ctctgccgcc gttataagat ggtatgccga aagggatgct gaaattgaga acgaaaagct
     1441 gcgccgggaa gttgaagaac tgcggcaggc cagcgagaca gatctccagc cagggactat
     1501 tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac gcacaggagc tgaaaaatgc
     1561 cagagactcc gctgaagtgg tggaaaccgc attctgtact ttcgtgctgt cgcggatcgc
     1621 aggtgaaatt gccagtattc tcgacgggat ccccctgtcg gtgcagcggc gttttccgga
     1681 actggaaaac cgacatgttg atttcctgaa acgggatatc atcaaagcca tgaacaaagc
     1741 agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac agtcaggtta
     1801 acaggctgcg gcattttgtc cgcgccgggc ttcgctcact gttcaggccg gagccacaga
     1861 ccgccgttga atgggcggat gctaattact atctcccgaa agaatccgca taccaggaag
     1921 ggcgctggga aacactgccc tttcagcggg ccatcatgaa tgcgatgggc agcgactaca
     1981 tccgtgaggt gaatgtggtg aagtctgccc gtgtcggtta ttccaaaatg ctgctgggtg
     2041 tttatgccta ctttatagag cataagcagc gcaacacact tatctggttg ccgacggatg
     2101 gtgatgccga gaactttatg aaaacccacg ttgagccgac catccgcgat attccgtcgc
     2161 tgctggcgct ggctccgtgg tatggcaaaa agcaccggga taacacgctc actatgaagc
     2221 gtttttccaa tggtcgtggc ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg
     2281 aaaagtcggt ggatgtggcg ggttatgatg aacttgctgc ctttgatgag gatattgaac
     2341 aggaaggctc tccgacgttc cttggcgaca aacgtattga aggctcggtc tggccaaagt
     2401 ccatccgtgg ctccacgccc aaagtgagag gcacctgcca gattgagcgt gcagccagtg
     2461 aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtatc
     2521 ttaaatttgg cgacaaagag acgccgtttg gcctcaaatg gacgccggat gacccctcca
     2581 gcgtgtttta tctctgcgag cataatgcct gcgtcatccg ccagcaggag ctggacttta
     2641 ctgatgcccg ttatatctgc gaaaagaccg ggatctggac ccgtgatggc attctctggt
     2701 tttcgtcatc cggtgaagag attgagccac ctgacagtgt gacctttcac atctggacag
     2761 cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaaa acgaaagggg
     2821 atacgggaaa acgtaaaacc ttcgtaaaca ccacgctcgg tgagacgtgg gaggcgaaaa
     2881 ttggcgaacg tccggatgct gaagtgatgg cagagcggaa agagcattat tcagcgcccg
     2941 ttcctgaccg tgtggcttac ctgaccgccg gtatcgactc ccagcgggat cgctacgaaa
     3001 tgcgcgtatg gggatggggg ccgggtgagg aaagctggct gattgatcgg cagattatta
     3061 tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc aataaaacct
     3121 atacccgccg gaatggtgca gaaatgtcga tatcccgtat ctgctgggat attggcggga
     3181 ttgacccgac cattgtgtat gaacgctcga aaaagcatgg gctgttccgg gtgatcccca
     3241 ttaaaggggc atccgtctac ggtaagccgg tggccagcat gcctcgtaag cgaaacaaaa
     3301 acggggttta ccttaccgaa atcggtacgg ataccgcgaa agagcagatt tataaccgct
     3361 tcacactgac gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc ccgaataacc
     3421 cggatatttt tgatctgacc gaagcgcagc agctgactgc tgaagagcag gtcgaaaaat
     3481 gggtggatgg caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat gaggcgctcg
     3541 actgcttcgt ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg cagctggatc
     3601 tcagtgcact gctggcgagc ctgcaggaag aggatggtgc agcaaccaac aagaaaacac
     3661 tggcagatta cgcccgtgcc ttatccggag aggatgaatg acgcgacagg aagaacttgc
     3721 cgctgcccgt gcggcactgc atgacctgat gacaggtaaa cgggtggcaa cggtacagaa
     3781 agacggacgg cgagtggagt ttacggccac ttccgtgtct gacctgaaaa aatacattgc
     3841 ggagctggag gtgcagaccg gcatgacaca gcgacgcagg ggacctgcag gattttatgt
     3901 atgaaaacgc ccaccattcc cacccttctg ggaccggacg gcatgacatc gctgcgtgaa
     3961 tatgccggtt atcacggcgg tggcagcgga tttggtgggc agttgcgggc gtggaaccca
     4021 ccgggtgaaa gtgtggatgc agccctgctg cccaacttta cccgtggcaa tgcccgcgca
     4081 gacgatctgg tacgcaataa cggctatgct gccaacgcca tccagctgca tcaggatcat
     4141 atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg
     4201 gaggaagacg cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag
     4261 gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa
     4321 ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt
     4381 tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg
     4441 aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg
     4501 gcgctgggat attacgtcag cgaggacggc tatcctggct ggatgccgca gaaatggaca
     4561 tggatacccc gtgagttacc cggcgggcgc gcctcgttca ttcacgtttt tgaacccgtg
     4621 gaggacgggc agacccgcgg tgcaaatgtg ttttacagcg taatggagca gatgaagatg
     4681 ctcgacacgc tgcagaacac gcagctgcag agcgccattg tgaaggcgat gtatgccgcc
     4741 accattgaaa gtgagctgga tacgcagtca gcgatggatt ttattctggg cgcgaacagt
     4801 aaggagcagc gggaaaggct gacgggctgg attggtgaaa ttgccgcgta ttacgccgca
     4861 gcaccggtcc gtctgggagg cgcaaaagtg ccgcacctga tgccggggga ctcactgaac
     4921 ctgcagacgg ctcaggacac ggataacggc tactccgtgt ttgagcagtc actgttgcgg
     4981 tatatcgctg ccgggctggg tgtctcgtat gagcaacttt cccggaatta cgcccagatg
     5041 agctactcca cggcacgggc cagtgcgaac gagtcgtggg cgtactttat ggggcggcga
     5101 aaattcgtcg catcccgtca ggcgagccag atgtttctgt gctggctgga agaggccatc
     5161 gttcgccgcg tggtgacgtt accttcaaaa gcgcgcttca gctttcagga agcccgcagt
     5221 gcctggggga actgcgactg gataggctcc ggtcgtatgg ccatcgatgg tctgaaagaa
     5281 gttcaggaag cggtgatgct gatagaagcc ggactgagca cctacgagaa agagtgcgcg
     5341 aaacgcggtg acgactatca ggaaattttt gcccagcagg tccgtgaaac gatggagcgc
     5401 cgcgcagctg gtcttaaacc gcccgcctgg gcggctgcgg catttgaatc cgggctgcga
     5461 caatcaacag aggaggagaa gagtgacagc agagctgcgt aatctcccgc atattgccag
     5521 catggctttt aatgagccgc tgatgcttga acccgcctat gcgcgggttt tcttttgtgc
     5581 gcttgcaggc cagcttggga tcagctgcct gacggatgca gtatccggcg acagcctgac
     5641 tgccggagag gcacccgcgg cgctggcgtt atccggtgat gatgacggac cacgacaggc
     5701 ccgcagttat caggtaatga acggcatcgc cgtgctgccg gtgtccggca cgctggtcag
     5761 ccggacgcgg gcgctgcagc cgtactcggg gatgaccggt tacaacggca ttatcgcccg
     5821 tctgcaacag gctgccagcg acccgatggt ggacggcatt ctgctcgata tggacacacc
     5881 gggcggaatg gtggcgggag catttgactg cgctgacatc atcgcccgtg tgcgtgacat
     5941 aaagccggta tgggcgctgg ccaatgacat gaactgcagc gcaggtcagc tgcttgccag
     6001 tgccgcctcc cggcgtctgg tcacgcagac cgcccggaca ggctccatcg gcgtcatgat
     6061 ggctcacagt aattacggcg ctgcactgga gaaacagggc gtggaaatca cgctgattta
     6121 cagcggcagc cataaggtgg atggcaaccc ctacagccat ctaccggatg atgtccggga
     6181 aacattgcag tcccggatgg atgcaacccg ccggatgttt gcacagaagg tgtcggcata
     6241 taccggcctg tccgtgcagg ctgtgctgga taccgaggct gcagtgtaca gcggtcagga
     6301 ggccattgat gccggactgg ctgatgaact tgttaacagt accgatgcga tcaccgtcat
     6361 gcgtgatgca ctggatgcac gtaaatcccg tctctcagga gggcgaatga ccaaagagac
     6421 tcaatcaaca actgtttcag ccactgcttc gcaggctgac gttactggcg tggtgcaagc
     6481 gacggagggc gagaacgcca gcgcggcgca gccggacgtg aacgcgcaga tcaccgcagc
     6541 ggttgcggca gaaaacagtc gcattatggg gatcctcaac tgtgaggagg ctcacggacg
     6601 cgaagaacag gcacgcgtgc tggccgaaac ccccggtatg accgtggaaa cggcccgccg
     6661 tattctggcc gcagcaccac agagtgcaca gacgcgcagt gacactgcgc tggatcgtct
     6721 gatgcagggg gcaccggcac cgctggctgc aggtaacccg gcatctgatg ccgttaacga
     6781 tttgctgaac acaccagtgt aagggatgtt tatgacgagc aaagaaacct ttacccatta
     6841 ccagccgctg ggcaatagtg acccggctca taccgcaacc gcgcccggcg gattgagtgc
     6901 gaaagcgcct gcaatgaccc cgctgatgct ggacacctcc acccgtaagc tggttgcgtg
     6961 ggatggcacc accgacggtg ctgccgttgg cattcttgcg gttgctgctg accagaccag
     7021 caccacactg acgttctaca agtccggcac gttccgttat gaggatgtgc tctggccgga
     7081 ggctgccagc gacgagacga aaaaacggac cgtgtttgcc ggaacggcaa tcagcatcgt
     7141 ttaactttac ccttcatcac taaaggccgc ctgtgtggct ttttttacgg gattttttta
     7201 tgtcgatgta cacaaccgcc cagctgctgg cggcaaatga gcagaaattt aagtttgatc
     7261 cgctgtttct gcgtctcttt ttccgtgaga gctatccctt cactacggag aaagtctatc
     7321 tctcacaaat tccgggactg gtaaacatgg cgctgtacgt ttcgccgatt gtttccggtg
     7381 aggttatccg ttcccgtggc ggctccacct ctgaatttac gccgggatat gtcaaaccca
     7441 agcatgaggt gaatccgcag atgaccctgc gtcgcctgcc ggatgaagat ccgcagaatc
     7501 tggcggaccc ggcttaccgc cgccgtcgca tcatcatgca gaacatgcgt gacgaagagc
     7561 tggccattgc tcaggtcgaa gagatgcagg cagtttctgc cgtgctcaag ggcaaataca
     7621 ccatgaccgg tgaagccttc gatccggttg aggtggatat gggccgcagt gcggcgaaca
     7681 acatcacaca gtccggcgtc acggagtgga gcaagcgtga caagtccacg tatgacccga
     7741 ccgacgatat cgaagcctac gcgctgaacg ccagcggtgt ggtgaatatc atcgtgtttg
     7801 acccgaaagg ctgggcgctg ttccgttcct tcaaagccgt caaggagaag ctggataccc
     7861 gtcgcggctc tcattccgag ctggagacag cggtaaaaga cctgggcaaa gcggtgtcct
     7921 ataagggaat gtatggcgat gtggccatcg tcgtgtattc cggacagtac gtggaaaacg
     7981 gcgtcaaaaa gaacttcctg ccggacaaca cgatggtgct ggggaacact caggctcgcg
     8041 gtctgcgcac ctatggctgc attcaggatg cggacgcaca gcgcgaaggc attaacgctt
     8101 ctgcccgtta cccgaaaaac tgggtgacca ccggcgatcc ggcgcgtgag ttcaccatga
     8161 ttcagtcagc accgctgatg ctgctggctg atcctgatga gttcgtgtcc gttcaactgg
     8221 cgtaatcatg gcccttcggg gccattttct ctctgtggag gagttcatga cgaaagatga
     8281 actgattgcc cgtcttcagg agctgggtga gcaattgaac cgcgatgtca gtttgacggg
     8341 aacgaaagaa gaactggcgc ttcgtgtggc agagctggaa gaagagcttg atgacacgga
     8401 tgaaactgcc ggtcaggaca cccctctcag ccgggaaaat gtgctgaccg ggcatgaaaa
     8461 tgaggtggta tcagcgcagc cggataccgt gattcaggat acggctgaac tggtcacggt
     8521 cgtggcactg gtgacgctgc atactgatgc acttcacgcc acgcgggata aacctgtggc
     8581 atttgtgctg ccgggaacgg cgtttcgtgt ctctgccggt gtggcagccg aaatgacaga
     8641 gcgcggcctg gccagaatgc aataacggga ggcgctgtgg ctgatttcga taacctgttc
     8701 gatgctgcca ttgcccgcgc cgatgaaacg atacgcgggt acatgggaac gtcagccacc
     8761 atgacatccg gtgagcagtc aggtgcggtg atacgtggtg tttttgatga ccctgaaaat
     8821 atcagctatg ccggacaggg cgtgcgcgtt gaaggctcca gcccgtccct gtttgtccgg
     8881 actgatgagg tgcggcagct gcggcgtgga gacacgctga ccatcggtga ggaaaatttc
     8941 tgggtagatc gggtttcgcc ggatgatggc ggaagttgtc atctctggct tggacggggc
     9001 gtaccgcctg ccgttaaccg tcgccgctga aagggggatg tatggccata aaaggtcttg
     9061 agcaggccgt tgaaaacctc agccgtatca gcaaaacggc ggtgcccggt gccgccgcaa
     9121 tggccattaa ccgcgttgct tcatccgcga tatcgcagtc tgcgtcacag gttgcccgtg
     9181 agacaaaggt acgccggaaa ctggtaaagg aaagggccag gctgaaaagg gccacggtca
     9241 aaaatccgca ggccagaatc aaagttaacc ggggggattt gcccgtaatc aagctgggta
     9301 atgcgcgggt tgtcctgtcc cgccgcaggc gtcataaaaa ggggcagcgt tcatccctga
     9361 aaggtggcgg cagcgtgctt gtggtgggta accgtcgtat tcccggcgcg tttattcagc
     9421 aactgaaaaa cggccggtgg catgttatgc agcgtgtggc cgggaaaaac cgttacccca
     9481 ttgatgtggt gaaaatcccg atggcggtgc cgctgaccac ggcgtttaaa cagaatattg
     9541 aacggatacg gcgtgaacgt cttccgaaag agctgggcta tgcgctgcag catcaactga
     9601 gaatggtaat aaagcgatga aacatactga actccgtgca gccgtactgg atgcactgga
     9661 gaagcatgac accggggcga cgctttttga tggtcgcccc gctgtttttg atgaggaaga
     9721 ttttccggca attgccgttt atctcaccgg cgctgaatac acgggcgaag agctggacag
     9781 cgatacctgg caggcggagc tgcatattga agttttcctg cctgctcagg tgccggattc
     9841 agagctggat tcgtggatgg agtcccggat ttatccggtg atgagcgata tcccggcact
     9901 gtcagatttg atcaccagta tggtggccag cggctatgac taccggcgcg acgatgatgc
     9961 gggcctgtgg agttcagccg atctgactta tgtcattatc tatgaaatgt gaggacgcta
    10021 tgcctgtacc aaatcctaca atgccggtga aaggtgccgg gaccaccctg tgggtttata
    10081 aggggagcgg tgacccttat gcgaacccgc tttcagacgt tgactggtcg cgtctggcaa
    10141 aagttaaaga cctgacgccc ggcgaactga ccgctgagtc ctatgacgac agctatctcg
    10201 atgatgaaga tgcagactgg actgcgaccg ggcaggggca gaaatctgcc ggagatacca
    10261 gcttcacgct ggcgtggatg cccggagagc aggggcagca ggcgctgctg gcgtggttta
    10321 atgaaggtga tacccgtgcc tataaaatcc gcttcccgaa cggcacggtc gatgtgttcc
    10381 gtggctgggt cagcagtatc ggtaaggcgg tgacggcgaa ggaggtgatt acccgcacgg
    10441 tgaaggtcac caatgtggga cgtccgtcga tggcagaaga tcgcagcacg gtgacggcga
    10501 cgaccggcat gacggtgaca cccgccagtg cttccgtagt gaaagggcag agcaccacgc
    10561 tgaccgtggc attccagccg gatggcgcaa ccgacaagag cttccgtgcg gtgtctgcgg
    10621 ataaaacaaa agccaccgtg tcggtcagtg gtatgaccat caccgtgaaa ggtgttgctg
    10681 caggcaaggt caacattccg gtcgtatctg gtaatggtga gtttgctgcg gttgcagaaa
    10741 tcaacgtcac cgccagttaa tccggagagt cagcgatgtt cctgaaaacc gaatcatttg
    10801 aacataacgg tgtgaccgtc acgctttctg aactgtcagc cctgcagcgt attgagcatc
    10861 tcgacctgat gaaacggcag gcagaacagg cggagtcaga cagcaaccgg aagtttactg
    10921 tggaagacgc catcagaacc ggcgcttttg tggttgcgat gtccctgtgg cataaccatc
    10981 cgaagaagac gcagatgccg tccatgaatg aagccgttaa acagattgag caggaagtgc
    11041 ttaccacctg gcccacagag gcaatttctc atgctgaaaa cgtggtgtac cggctgtccg
    11101 gtatgtatga gtttgtggtg aatgatgctc ctgaacaggc agaggacgcc gggcctgcag
    11161 agcctgtttc tgcgggaaag tgttcgacgg tgagctgagt tttgccctga aactggcgcg
    11221 tgagatgggg cgacccgact ggcgcgccat gcttgccggg atgtcatcca cggagtatgc
    11281 cgactggcac cgcttttaca gtacccatta ttttcatgat gttctgctgg atatgcactt
    11341 ttccgggctg acgtacaccg tgctcagcct gtttttcagc gatccggaga tgcatccgct
    11401 ggatttcagt ctgctgaacc ggcgcgaggc tgacgaagag cctgaagatg atgtgctgat
    11461 gcagaaagcg gcagggcttg ccggaggtgt ccgctttggc ccggacggga atgaagttat
    11521 ccccgcttcc ccggatgtgg cggacatgac ggaggatgac gtaatgctga tgacagtatc
    11581 agaagggatc gcaggaggag tccggtatgg ctgaaccggt aggcgatctg gtcgttgatt
    11641 tgagtctgga tgcggccaga tttgacgagc agatggccag agtcaggcgt catttttccg
    11701 gtacggaaag tgatgcgaaa aaaacagcgg cagtcgttga acagtcgctg agccgacagg
    11761 cgctggctgc acagaaagcg gggatttccg tcgggcagta taaagccgcc atgcgtatgc
    11821 tgcctgcaca gttcaccgac gtggccacgc agcttgcagg ggggcagaat ccctggctga
    11881 tcctgctgca acagggtggt caggttaagg actccttcgg cgggatgatc cccatgttca
    11941 gggggcttgc cggcgcgatc accctgccga tggtgggggc cacctcgctg gcggtggcga
    12001 ccggtgcgct ggcgtatgcc tggtatcagg gcaactcaac cctgtccgat ttcaacaaaa
    12061 cgctggtcct ttccggtaat caggcgggac tgacggcaga tcgtatgctg gtcctgtcca
    12121 gagccgggca ggcggcaggg ctgacgttta accagaccag cgagtcactc agcgcactgg
    12181 ttaaggcggg ggtaagcggt gaggctcaga ttgcgtccat cagccagagt gtggcgcgtt
    12241 tctcctatgc atccggcgtg gaggtggaca aggtcgctga agccttcggg aagctgacca
    12301 cagacccgac gtcggggctg acggcgatgg cacgccagtt ccataacgtg acggcggagc
    12361 agattgcgta tgttgctcag ttgcagcgtt ccggcgatga agccggggca ttgcaggcgg
    12421 cgaacgaggc cgcaacgaaa gggtttgatg accagacccg ccgcctgaaa gagaacatgg
    12481 gcacgctgga gacctgggca gacaggactg cacgtgcatt caaatccatg tgggatgcgg
    12541 tgctggatat tggtcgtcct gataccgcgc aggagatgct gattaaggca gaggctgcgt
    12601 ttaagaaagc agacgacatc tggaatctgc gcaaggatga ttatttcgtt aacgatgaag
    12661 cacgggcgcg ttactgggat gatcgtgaaa aaaaacgcct ggagcgggat gcagcccaaa
    12721 agagggtaga tcaacagcgt caacaggaca aaaatgcgca gcagcagagc gataccgaag
    12781 cgtcacggct gaaatatacc gaagaggcgc agaaggctta cgaacggctg cagacgccgc
    12841 tggagaaata taccgcccgt caggaagaac tgaacaaggc actgaaagac ggaaaaatcc
    12901 tgcaggcaga ttacaacacg ctgatggcgg cggcgaaaaa ggattatgaa gcgacgctga
    12961 aaaagccgaa acagtccggc gtgaaggtgt atgcgggcga tcgtcaggaa gacagtgctc
    13021 atgcggccct gctgacgctt caggcagaac tccggacgct ggagaagcat gccggagcga
    13081 atgagaaaat cagccagcag cgccgggatt tgtggaaggc agaaagtcag ttcgcggtac
    13141 tggaggaggc ggcacaacgt cgccagctgt ccgcacagga gaaatccctg ctggcgcata
    13201 aagacgagac gctggagtac aaacgccagc tggctgcact tggtgacaag gttacgtatc
    13261 aggagcacct gaatgcgctg gcgcagcagg cggataaatt cgcacagcag caacgggcaa
    13321 aacgggcagc cattgatgcg aaaaaccggg ggctgactga ccggcaggca gcgcgggaag
    13381 ccacggaaca gcgcctgaag gaacagtatg gcgataatcc tctggcgctg aataacgtca
    13441 tgtcagagca gaaaaagacc tgggcggctg aagaccagct tcgcgggagc tggatggcag
    13501 gcctgaagtc cggctggagt gagtgggaag agagcgccac ggacagtatg tcacaggtta
    13561 aaagtgcagc cacgcagacc tttgatggta ttgcacagaa tatggcggcg atgctgaccg
    13621 gcagtgaaca gaactggcgc agcttcaccc gctccgtgct gtccatgatg acagaaattc
    13681 tgcttaagca ggcaatggtg gggattgtcg ggagtatcgg cagcgccatt ggcggggctg
    13741 ttggtggcgg cgcatccgcg tcaggtggta cagccattca ggccgctgcg gtgaaattcc
    13801 attttgcaac cgggggattt acgggaaccg gcggcaaata tgagccagcg gggattgttc
    13861 accgtggtga atttgtcttc acgaaggagg caaccagccg gattggcgtg gggaatctct
    13921 accggctgat gcgcggctat gcggaaggtg gttatgtggg cggtgccgga agtccggcgc
    13981 agatgcggcg ggctgaaggc attaatttta atcagaacaa tcacgtggtg attcagaacg
    14041 acggtacgaa tggtctgcca ggtccacaga tgatgaaggc cgtgtatgac atggcccgca
    14101 agggtgcccg tgatgaaatt cagacacaga tgcgtgatgg tggcctgttc tccggaggtg
    14161 gacgatgaaa accttccgct ggaaagtgaa acccggtatg gatgtggctt cggccccttc
    14221 tgtaagaaag gtgcgctttg gtgatggcta ttcccagcga gcgcctgccg ggctgaatgc
    14281 caacctgaaa acgtacagcg tgacgctttc tgtcccccgt gaggaggcca tggtactgga
    14341 gtcgtttctg gaagagcacg ggggctggaa agcctttctg tggacgccgc cttatgagtg
    14401 gcggcagata aaggtgacct gcgcaaaatg gtcgtcgcgg gtcagtatgc tgcgtgttga
    14461 gttcagcgca gagtttgaac aggtggtgaa ctgatgcagg atatccggca ggaaacactg
    14521 aatgaatgca cccgtgcgga gcagtcggcc agcgtggtgc tctgggaaat cgatctgaca
    14581 gaggtcggtg gagaacgtta ttttttctgt aatgagcaga acgaaaaagg tgagccggtc
    14641 acctggcagg ggcgacagta tcagccgtat cccattcagg ggagtggttt tgaactgaat
    14701 ggcaaaggca ccagtacgcg ccccacgctg atggtttcta acctgtacgg tatggtcacc
    14761 gggatggcgg aagatctaca gagtctggtc ggcggaacgg tggtccggcg taaggtttac
    14821 gcccgttttc tggatgcggt gaacttcgtc aacggaaaca gtgacgccga tccggagcag
    14881 gaggtgatca gccgctggcg cattgagcag tgcagcgaac tgagcgcggt gagtgcctct
    14941 tttgtactgt ccacgccgac ggaaacagat ggcgctgttt ttccgggacg tatcatgctg
    15001 gccaacacct gcacctggac ctatcgcggc gatgagtgcg gttatcacgg tccggcggtc
    15061 gcggatgaat atgaccagcc aacgtccgac atcacgaagg ataaatgcag caaatgcctg
    15121 agtggctgta agtttcgcaa taacgtcggc aactttggcg gcttcctttc cattaacaaa
    15181 ctttcgcagt aaatcccatg acagagacag aatcagcgat tctggcgcac gcccggcgat
    15241 gtgctccagc ggagtcgtgc ggcttcgtgg taagaacgcc ggagggggaa agatatttcc
    15301 cctgcgtgaa tatctccggt gagccggagg cgtatttccg tatgtcgccg gaagactggc
    15361 tgcaggcaga gatgcagggt gagattgtgg cactggtcca cagccacccc ggtggtctgc
    15421 cctggctgag tgaggccgac cggcggctgc aggtgcagag tgatttgccg tggtggctgg
    15481 tctgccgggg ggcgattcac aagttccgct gtgtgccaca tcttaccggg cggcgctttg
    15541 agcacggggt gacggactgt tacacgctgt tccgggatgc ttaccatctg gcggggattg
    15601 agatgccgga ttttcatcgt gaggatgact ggtggcgtca cggtcagaat ctctatctgg
    15661 ataatctgga ggccacaggg ctgtatcagg tgccgttgtc agcggcgcag ccgggcgatg
    15721 tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac tgtggtgacg
    15781 gcgagctgct gcaccatatt cctgaacaac tgagcaaacg agagaggtat accgacaaat
    15841 ggcagcgacg cacacactcc ctctggcgtc accgggcatg gcacgcatct gcctttacgg
    15901 ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg aaaacgggtg ccgaagccat
    15961 ccgggcgatg gccacacagc tccctgcgtt tcgtcagaag ctgagcgacg gctggtatca
    16021 ggtgcgtatt gccgggcagg atgttagcac gtccggatta acggcacagt tacatgaggc
    16081 tctgcctgac ggcgctgtga ttcatattgt tcccagagtc gccggggcca agtcaggtgg
    16141 tgtattccag attgtcctgg gagcagccgc cattgccgga tcattcttta ccgccggagc
    16201 cacccttgca gcatgggggg ctgccattgg tgccggtggt atgaccggca ttctgttttc
    16261 tctcggtgcc agtatggtgc tcggcggtgt ggctcagatg ctggcaccga aagccagaac
    16321 tccccgcaca cagacaacgg ataacggtaa gcagaacacc tatttctcct cactggataa
    16381 catggttgcc cagggcaatg ttatgcctgt tctgtatggt gaaatgcgcg tggggtcacg
    16441 tgtggtatct caggagatca gcacggcaga cgaaggggac ggtggtcagg ttgtggtgat
    16501 tggtcgctga tgcaaaatgt tttatgtgaa atcgcctccg ggcggtttta tcgtttatgg
    16561 agcatgacga atgggtaaag gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa
    16621 cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc agcgaagggc cggttgaagg
    16681 tccggtggat ggattaaaaa gcgtgctgct gaacagtacg ccggtgctgg acagtgaggg
    16741 gaataccaac atctccggcg tcacggtggt gttccgggca ggtgagcagg agcagtcacc
    16801 gccggaggga tttgaatcct ccggctccga gacggtgctg ggtacggaag tgaaatatga
    16861 cacgccgatc acccggacca tcacgtcggc aaacattgac cgactgcgtt ttaccttcgg
    16921 tgtacaggca ctggtggaaa ccacctcaaa gggagacagg aatccgtctg aagttcgcct
    16981 gctggttcag atacagcgta acggtggctg ggtgacggaa aaagacatca ccattaaggg
    17041 caaaaccacc tcgcagtatc tggcctcggt ggtggtggat aacctgccgc cgcgcccgtt
    17101 taatatccgg atgcgcagaa tgacgccgga cagcaccaca gaccagctgc agaacaaaac
    17161 gctctggtcg tcatacaccg aaatcatcga tgtgaaacag tgctacccga acacggcact
    17221 ggtcggcgta caggtggatt cggagcagtt cggcagccag caggtgagcc gtaattatca
    17281 tcttcgcggg cgcattctgc aggtgccgtc gaactataat ccgcagacgc ggcaatacag
    17341 cggtatctgg gacggaacgt taaaaccggc atacagcaac aacatggcct ggtgtctgtg
    17401 ggatatgctg acccatccgc gctatggcat ggggaaacgt cttggtgcgg cagatgtgga
    17461 taaatgggcg ctgtatgtca tcggccagta ctgtgaccag tcggtgccgg acggctttgg
    17521 cagcacggag ccgcgcatca cctgtaatgc ctggctgacc acacagcgca aggcgtggga
    17581 tgtgctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct
    17641 gacgttcgtg caggaccgac agtcggataa ggtgtgggcc tataaccgca gtaatgtggt
    17701 gatgccggat gatggcgcgc cgttccgcta cagtttcagc gccctgaaag accgccataa
    17761 tgccgttgag gtgaactgga ttgacccgga taacggctgg gagacggcga cagagcttgt
    17821 ggaggatacg caggccattg tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg
    17881 ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa cggaactgct
    17941 ggagacgcag accgtggatt tcagcgtggg tgctgaaggg cttcgtcatg taccgggcga
    18001 tgtcattgaa atctgcgatg atgactatgc cggtatccgc accggcgggc gcgtgctggc
    18061 ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg
    18121 caccacgctg ataagcctgg ttgacgggca ggggaatccg gtcagcgtgg aggtccagtc
    18181 cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg ctgaatacag
    18241 cgtgtggggg ctgaagctgc cgacgctgca ccagcgattg ttccgctgcg tgagtatccg
    18301 tgagaacgac gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaagc
    18361 catcgtggat aacggggcgc actttgacgg cgaccagagc ggcacggtga atggtgtcac
    18421 gccgccagcg gtgcagcacc tgactgccga agtcaccgca gacagcgggg aatatcaggt
    18481 gctggcgcgc tgggacacgc cgaaggtggt gaagggcgtg agcttcctgc tccgtctgac
    18541 cgtaacagcg gatgacggca gtgagcggct ggtcagcacg gcccggacga cggaaaccac
    18601 ttaccgcttc acacaactgg cgctggggaa ctacaggctg acagtccggg cagtaaatgc
    18661 gtgggggcag cagggcgatc cggcgtcggt atcgttccgg attgccgcac cggcagcgct
    18721 gtcgcggatt gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgcggt
    18781 ttatgatccg acggtacagt ttgagttctg gttctcggaa aagcggattg cggatatcag
    18841 gcaggttgaa accacagcac gctatcttgg cacggcgctg tactggatag ccgccagtat
    18901 caatatcaaa ccgggccatg attattactt ttatatccgc agtgtgaaca ccgttggcaa
    18961 atcggcattc gtggaggctg ttggtcagcc gagtgatgat gcatccggtt atctggattt
    19021 tttcaaaggc gagataggga aaacccatct ggctcaggag ctgtggacgc agattgataa
    19081 cggtcagctt gcgcctgacc tggctgaaat caggacgtcc attacggatg tcagcaatga
    19141 aatcacgcag accgtcaata agaaactgga agaccagagt gcggcaattc agcagataca
    19201 gaaggttcag gttgatacaa ataataacct gaacagcatg tgggctgtga agctgcagca
    19261 gatgcaggac ggacgccttt atatcgcggg tattggtgcc ggtattgaga acacccctga
    19321 cggcatgcag agtcaggtgc tgctggcggc ggacaggatt gcgatggtta atcctgcgaa
    19381 tggcaacaca aaaccgatgt ttgttggtca gggcgatcag atattcatga acgacgtgtt
    19441 cctgaaacgc ctgacggccc ccaccattac cagcggtgga aatccatcgg cattttccct
    19501 gactccggac ggaaagctga ccgctaaaaa tgcggatatc agtggcagtg tgaatgcgaa
    19561 cgccgggacg ctcaacaatg tcacggtaaa tgaaaactgt acgattaagg gcatgctgga
    19621 ggcgactcag gtcagaggtg acttcgttaa agctgtatcc aaatcatttc cgaaacaggc
    19681 tggtacgtgg ggtaacacgg aaacaccaaa cgggacggtt acagtcacca taagcgatga
    19741 tcataacttt gaccgccaga ttattattcc gcccattatt tttaacggta tagcgtatga
    19801 cgatccgggg agcggaaata acccaggagg cacgcgatac acgggttatg gttttgaagt
    19861 tcgcaaaaac ggcgtattaa tcgcatccag agaaactaaa ggtgccattc ccggtagtta
    19921 cagtgcagtt attgatatgc ctagtggtgg tggtagcgtc actctggagt ttaagatttt
    19981 ccagaaaggc aatcaggggg caggcaatat caccgactgt acggtgattg tgaccaaaaa
    20041 agcggcttcc ggcatcagta ttcgttgaaa tatttataac cccaataaat ggcgtcagga
    20101 atgacgcctt ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca
    20161 ttttgtccgc agccatttgc ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc
    20221 agtccacgct gagcgcgggg tatcttcatg cccggaccaa cgttcccggc agtgatgatc
    20281 tgaacgggat taacgtgaaa taccgttatg agtttacgga tacgctgggg ctggtgacgt
    20341 cattcagcta tgcaggagac aagaatcgcc agctgacccg ttacagcgat acccgctggc
    20401 atgaagattc cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga
    20461 atgaatggtt cagcgcgtat gcgatggcgg gtgtggctta cagccgtgtg tcgacttttt
    20521 ccggggatta tatccgcgta actgacaata aggggaaaac gcacgatgtg ctgaccggaa
    20581 gtgatgacgg tcgccacagc aacacgtctc tggcgcactg acggtttcat cgtgggtgtc
    20641 ggttataaat tctgattagc caggtaacac agtgttatga cagcccgccg gttcaggcgg
    20701 gcttttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag acggcacagg
    20761 aaaaccggta cagaactgca caatccagct gaaagcaaaa cgtaacagca ccacggtggt
    20821 ggtgaacacg ctggcctcag aaaatccgga tgaagccggg cgttacagca tggacgttga
    20881 gtacggtcag tacagcgtta ttctgttggt ggaaggattc ccgccgtcac atgccgggac
    20941 catcaccgtg tatgaagatt cccgacccgg tacgctgaat gattttctcg gtgccatgac
    21001 ggaggatgat gcccgtccgg aggcactgcg ccgttttgag ctgatggtgg aagaggtggc
    21061 gcgtaacgcg tccgcggtgg cacagaacac ggcagccgcg aagaaatcag ccagcgatgc
    21121 cagcacatca gcccgtgagg cggcaaccca tgcgactgat gctgcagatt cagcacgtgc
    21181 agccagcacg tcagccggac aggccgcttc gtcggctcag tcagcgtctt ccagcgcagg
    21241 aacggcatca acaaaggcca ctgaagcctc aaaaagtgct gccgctgcag agtcctcaaa
    21301 aagcgcggcg gctaccagtg ccggtgcggc gaaaacgtta gaaacgaatg cggcagcgtc
    21361 acaacaatca gcagccactt ctgcatccac cgcgaccacg aaagcgtcag aagctgccac
    21421 ctcagcccgg gatgcgtcgg cttcaaaaga ggcggcaaaa tcatcagaaa cgaacgcatc
    21481 atcaagcgcc agtagcgcag cttcctcggc gacggcggct gcaaattctg cgaaggcggc
    21541 caaaacgtcc gagacgaacg ccaggtcttc tgaaacggca gcgggacaga gtgcctcagc
    21601 tgcggcaggc tcaaaaacag cggctgcgtc gtctgccagc gccgcgtcaa caagtgccgg
    21661 gcaggcctca gccagtgcca ccgccgccgg aaaatcggca gaaagcgccg catcatccgc
    21721 ttcaacagcc acaacgaagg ctggcgaagc cactgagcag gccacggcag cagcgaggtc
    21781 agcttccgca gcgaagacat ccgaaacgaa cgcgaaagcg tcggaaaccc gtgcagaatc
    21841 ctcaaaaacg gctgccgcat cgtccgccag ttcggcggcg tcatcggcat catcggcgtc
    21901 tgcttcaaaa gatgaggcga ccagacaggc gtcagcagcg aagggcagcg ccacgacggc
    21961 atccacgaag gcgacagagg ctgctggtag tgcgacggca gcagctcaga gcaaaagtac
    22021 ggcggaatct gcagcaacgc gcgctgagac agcggcaaaa cgggcagagg atattgcatc
    22081 cgccgtggcg cttgaggatg cgagcacgac gaaaaagggg atagtacagc tcagtagtgc
    22141 gactaacagc acttccgagt cactggcggc aacaccaaaa gccgttaagg ccgcgtatga
    22201 cctggctaac gggaaataca ccgcacagga tgcaacgaca gcacagaaag ggataatcca
    22261 gctaagcagc gcgaccaaca gcacgtctga aacgctggcg gcaacgccaa aggcagtaaa
    22321 agcagccaat gacaatgctg agaaacgtct gcagaaagat cagaacggtg cggatatccc
    22381 tggcaaagac acctttacga aaaatattgg tgcctgccgt gccttcggtg ggtcagtaag
    22441 cacaacaaca ggaaactgga cgactgcaca gtttatcgag tggctggatt ctcagggagc
    22501 atttaaccat ccatactgga tgtgcaaggg ttcctggtct tatggcaata ataaaatcat
    22561 tactgatact ggctgcggta atattcatct cgccggagct gtcattgaag taatggggat
    22621 aaagtcagcg atgacgatcc gcattaccac tccgaccaca tccagcggtg gcggaacaac
    22681 caacgcccag tttacctata ttaatcacgg aacagattat tcacctggct ggcgaaggga
    22741 ctataactcc agaaataagc caacggcatc agagatcggg gcgctaccgt caggtggaac
    22801 agcagtatca tcagttaatc tggcttcaaa aggtcgggta gccgcgctga cagacaatac
    22861 gcagggggca gcaggtcttg agttatacga ggtgtataac aacggatatc caacagcgta
    22921 tggaaatatc attcacctga aagggatgac agccgttggc gaaggtgagt tactcatcgg
    22981 ctggagtggt acaagcggtg ctcatgctcc ggcatttatt cgttcacgac gggatacgac
    23041 cgacgcaaac tggtcgccgt gggcgcagct ttacacctcg gctcatcctc ctgcagagtt
    23101 ttatccagtc ggtgcaccaa tcccgtggcc atcagatacc gttccgtctg gttatgccct
    23161 gatgcagggg cagacttttg acaaatctgc ttacccgaaa cttgcagccg cttatccgtc
    23221 aggcgtgatc cctgatatgc gtggctggac gattaagggc aagcccgcca gtggtcgtgc
    23281 cgtattgtct caggaacagg atggcattaa atcgcacacc cacagcgcca gcgcatccag
    23341 tacggatttg gggacgaaaa ccacatcgtc gtttgattac ggcactaaat ccacgaataa
    23401 caccggggcg catacgcaca gtctgagcgg ctctacaagt tcagctgggg cccaccaaca
    23461 tagtcagact gggcccagaa cgaatagcgg gagtcaacct actggaatgt ttccggcagg
    23521 gagtactcaa gttagcggga ctaatcaggt tggtatcagt ggctcattaa ccagtggcac
    23581 ctcccaatgg gtaggtaaat catcttcaga aggaaaccat acacactcac tgtccggcac
    23641 agcagcatct gcgggtgcac acgcgcatac tgtcggtatt ggtgctcata cgcactctgt
    23701 tgcgattggt tcacatggac acaccatcac cgttaacgct gctggtaacg cggaaaacac
    23761 cgtcaaaaac atcgcattta actatattgt gaggctcgca taatggcatt cagaatgagt
    23821 gaacaagcac ggaccataaa aatttataac ctgctggccg gaactaatga atttattggt
    23881 gaaggtgacg catatattcc gcctcataca ggtctgcctg caaacagtac cgatattgca
    23941 ccgccagata ttccggctgg tttcgtggct gttttcaaca gtgatgagtc atcgtggcat
    24001 ctcgttgaag atcatcgggg taaaacggtt tatgacgtgg cttccggcga cgcgttattt
    24061 atttctgaac tcggctcatt accggaaaat gtcacctggt tatccccgga aggggagttt
    24121 cagaagtgga acggcacagc ctgggtgaag gatacggaag cagaaaaact gttccggatc
    24181 cgggaggcgg aagaaacaaa aaacagcctg atgcaggtag ccagtgagca tatagcgccg
    24241 cttcaggatg ctgtggatct ggagatcgca acggagaaag aaaccttgtt gctggaagct
    24301 tggaaaaagt atcgggtgtt gctgaaccgt gttgatacat caactgcacc ggatattgaa
    24361 tggccagtag cacctatagg gtaaattcgt aatgattacc taaatacgtt attcttttgt
    24421 taaaaaagtg attttgctat aggtagggag tatgttcttg gattaagaat aaaaaatcgc
    24481 catgaaagag cttcatggcg agtacataaa cgtacaaaat gtgatgaaag tagagtggcg
    24541 taagccacta tattaagata tgacaatagc gaagaaaagt aaataacatt ttatggacgt
    24601 ttaattgtta attataaaaa ctagagtgcc cgatattgtt tatcttgctt taaaataatg
    24661 tactcaaacc aaatttttga gctaaaagct caaataatat aaatgcaata ataatgttta
    24721 ataagacaat tagcttgctt tgtattttca tttcggaata ctgttaagta actttgatga
    24781 ccctaacaat agatcggaat tttcttagag tcaatggtca ctgtttttgc ttgcaaagca
    24841 aatgttttgg tggttataag tcgtcttaat atagtcacgc atgcaacttg ttccatgcgg
    24901 agattaaaga ttggaatata acgcgaagat ggaattaaat tacatttgca ataattaatt
    24961 tattattact caataattac cgatattatt attgtaaatt aatcattaga atgcaaaaag
    25021 aaatgtaacg gcaaaataag aggacgatag ctgagaaaaa agcgtgcttt cccaatctgg
    25081 ggcaaatcac gtctgaatga cacctgaaaa caggtggtag cctcaagaa
//
LOCUS       DAETQN010000058        25104 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.058, whole genome shotgun sequence.
ACCESSION   DAETQN010000058 DAETQN010000000
VERSION     DAETQN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.058"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            194..382
                     /locus_tag="K0W68_002337"
     CDS             194..382
                     /locus_tag="K0W68_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HBI3073878.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            394..1146
                     /gene="bcsQ"
                     /locus_tag="K0W68_002338"
     CDS             394..1146
                     /gene="bcsQ"
                     /locus_tag="K0W68_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBI3073879.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1143..1307
                     /locus_tag="K0W68_002339"
     CDS             1143..1307
                     /locus_tag="K0W68_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HBI3073880.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1500..3761
                     /gene="bcsA"
                     /locus_tag="K0W68_002340"
     CDS             1500..3761
                     /gene="bcsA"
                     /locus_tag="K0W68_002340"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HBI3073881.1"
                     /translation="MNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTST
                     LNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFV
                     PTYNENLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHE
                     HAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSP
                     DPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSYAVIRRKPLDEIGGIAVET
                     VTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPL
                     TGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPH
                     MIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVE
                     EEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGA
                     VAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQ
                     KVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQD
                     SYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRHPERS
                     ETAQPSDQALAQQ"
     gene            3772..6111
                     /gene="bcsB"
                     /locus_tag="K0W68_002341"
     CDS             3772..6111
                     /gene="bcsB"
                     /locus_tag="K0W68_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBI3073882.1"
                     /translation="MKRKLFWICAVAMRMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6118..7224
                     /gene="bcsZ"
                     /locus_tag="K0W68_002342"
     CDS             6118..7224
                     /gene="bcsZ"
                     /locus_tag="K0W68_002342"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBI3073883.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7206..10698
                     /gene="bcsC"
                     /locus_tag="K0W68_002343"
                     /pseudo
     CDS             7206..10698
                     /gene="bcsC"
                     /locus_tag="K0W68_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10780..12768
                     /gene="hmsP"
                     /locus_tag="K0W68_002344"
     CDS             10780..12768
                     /gene="hmsP"
                     /locus_tag="K0W68_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBI3073884.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12951..14237
                     /gene="dctA"
                     /locus_tag="K0W68_002345"
     CDS             12951..14237
                     /gene="dctA"
                     /locus_tag="K0W68_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HBI3073885.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14457..15953
                     /locus_tag="K0W68_002346"
     CDS             14457..15953
                     /locus_tag="K0W68_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBI3073886.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="K0W68_002347"
     CDS             complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="K0W68_002347"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HBI3073887.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17210..17977
                     /gene="pdeH"
                     /locus_tag="K0W68_002348"
     CDS             17210..17977
                     /gene="pdeH"
                     /locus_tag="K0W68_002348"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBI3073888.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18047..20107
                     /locus_tag="K0W68_002349"
     CDS             18047..20107
                     /locus_tag="K0W68_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBI3073889.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20289..21611)
                     /locus_tag="K0W68_002350"
     CDS             complement(20289..21611)
                     /locus_tag="K0W68_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBI3073890.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="K0W68_002351"
     CDS             complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="K0W68_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBI3073891.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23084..23983)
                     /locus_tag="K0W68_002352"
     CDS             complement(23084..23983)
                     /locus_tag="K0W68_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3073892.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24503..>25104
                     /locus_tag="K0W68_002353"
     CDS             24503..>25104
                     /locus_tag="K0W68_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI3073893.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5894 a         6365 c         6991 g         5854 t
ORIGIN      
        1 ttagcttgtg aatcaattta gaaggtcaca agacgtgtga ttttagtctt ttcgttttta
       61 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
      121 aataaaccgg cgaaatgctc acggttagaa ttgtttcatg aacggtacgg ttatttcata
      181 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
      241 tatcttccag aatgatattg tggcgttaaa gcaggcattt tcactgcctg atattgatta
      301 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
      361 agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg
      421 cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg
      481 ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct gtcatttaat
      541 gttgatttta cccaccgtca gggctgggcc agagcgatgc tggatggcca ggactggcgt
      601 gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt
      661 gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta
      721 cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc
      781 tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat cgtcaatgtg
      841 gatgccaact gccatatccg actgcatcag caagcgctgc cggatggcgc acatattttg
      901 attaatgact tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa
      961 agccagcgcc gattactgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg
     1021 gcggctaagc aaccagtagg tgaatatcgc agtgatgcgc tggcggctga agagatactg
     1081 acgctggcga actggtgcct gttgaactac tccgggctga aaacgccagt cgggagtgca
     1141 tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc
     1201 gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc ggctgtttct
     1261 ggatgatcct ggcctggatt tttattccgc tggagcaccc gcgctagcag cgtattcgcg
     1321 cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg ctggacccgg
     1381 tccgttatct cattcaaaca tgctggttac tgatcggtac atcgcgcaaa gaaacgccga
     1441 aaccgcgcag gcgggcattt tcaggtctgc agaatattcg tggacgttac catcaataga
     1501 tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag aaaaaagagc
     1561 tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc
     1621 tgattctggc gttaatctgc gttactcaac cgtttaaccc gctggcgcag tttatcttcc
     1681 tgatgctgct gtggggtgta gcgctgatcg tacggcggat gccggggcgc ttctcggcgc
     1741 taatgttgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg cgttacacct
     1801 ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg
     1861 aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc
     1921 gtcagccggt gccattgccg aaggatatgt cgctgtggcc gtcagtggat atctttgtcc
     1981 cgacttacaa cgaaaatctc aatgtggtga aaaataccat ttacgcctcg ctgggtatcg
     2041 actggccgaa agacaaactg aacatctgga tcctcgatga cggcggcagg gaagagtttc
     2101 gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg caccactcat gaacatgcga
     2161 aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg
     2221 actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag
     2281 agaaacagct ggcgatgatg cagactccac accacttctt ctcgccggac ccgtttgaac
     2341 gcaacctggg acgttttcgt aaaacgccga acgaaggcac gctgttctat ggtctggtgc
     2401 aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctatgcg gtgattcgtc
     2461 gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata
     2521 cttctctgcg tttgcaccgt cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg
     2581 cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg
     2641 ggatggtaca aatcttccgt ctcgataacc cgctcaccgg taaaggactg aagtttgctc
     2701 agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca cggctgatct
     2761 tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat gcgccagcgt
     2821 tgatgatcgc cctattcgtg ctgccgcata tgatccatgc cagcctgacc aactcgaaga
     2881 tccagggcaa atatcgccac tctttttgga gtgaaatcta cgaaacggtg ctggcgtggt
     2941 atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa tttaacgtca
     3001 ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg cggccctaca
     3061 tcttccttgt tctactcaac ctggtgggcg ttgcggtagg catctggcgc tacttctatg
     3121 gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtgttc tacaacctga
     3181 ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc
     3241 gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta
     3301 ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag gcgcagattc
     3361 tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac gtcttcccga
     3421 cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc
     3481 agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc
     3541 aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg aagctcggct
     3601 tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc atattccgtg
     3661 tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccacccg gagcggagcg
     3721 aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc gatgaaaaga
     3781 aaactattct ggatttgtgc agtggctatg aggatgagtg cgttcccctc tttcatgacg
     3841 caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc cgcccagacg
     3901 gaacaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgctga tgcgccagtc
     3961 gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat tgcgccgccg
     4021 ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt tggtatgcgc
     4081 agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc gccatcgtta
     4141 ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg cgtgctgcca
     4201 gtgaccaaag aacagttggg taaaaaaacg ctggcgcaaa tgcccattaa cccactgttt
     4261 attaccgact tcaaccgtgt gcggctggag tttgtcggcc attatcagga cgtgtgcgaa
     4321 aacccggcca gcaccacgct ttggctggat gttgggcgga gcagtggact ggatctgacc
     4381 tatcagaccc tgaatgtgaa gagtgacctg tcacacttcc cggtgccatt ctttgacccg
     4441 cgcgataacc gtactaacac cttgccgatg gtctttgcgg gtgcgccgga tgttgggctg
     4501 caacaagcat ctgccattgt cgcctcgtgg tttggttcgc gttctggctg gcgtgggcag
     4561 aacttcccgg tgctctataa ccaattgccg gatcgcaatg ccattgtctt tgcaaccaac
     4621 gacaaacggc cggacttcct gcgcgatcat ccggcggtaa aagccccggt gattgagatg
     4681 attaaccatc cgcagaatcc ttacgtcaaa ctgctggtgg tgtttggtcg tgacgacaaa
     4741 gacctgttgc aggcagcgaa aggtatcgct cagggtaaca ttctgttccg tggtgaaagc
     4801 gtggtagtga atgaagtgaa accgctgcta ccgcgtaagc cgtacgatgc gccgaactgg
     4861 gtacgtaccg atcgtccggt cacatttggc gaactgaaaa cctatgaaga acagttacaa
     4921 tccagcggtc ttgagccagc agcgattaac gtttcgctaa acctgccgcc ggatctctac
     4981 ctgatgcgca gtaccggcat tgatatggat attaattacc gctacaccat gccgccggtg
     5041 aaagacagtt cgcggatgga tatcagcctg aataaccagt tcctgcaatc cttcaacctg
     5101 agcagtaaac aggaggcgaa ccgcctgctg ctgcggattc cggtattaca aggtttgctg
     5161 gatggcaaaa cggatgtctc tattccggcg ctgaaactgg gcgcgaccaa ccagctgcgc
     5221 ttcgactttg agtatatgaa cccgatgccg ggcggttcgg tggataactg tattaccttc
     5281 cagccggtgc agaatcatgt ggtgattggt gacgattcca ccatcgactt ctcgaagtat
     5341 taccacttca tcccgatgcc ggatctacgc gcctttgcta acgcgggctt cccgttcagc
     5401 cggatggcgg atctgtcgca aaccatcacc gtgatgccga aaacgcctaa cgaagcacag
     5461 atggaaacgt tgctgaatac tgttggtttt atcggcgcac agacgggctt cccggcgatt
     5521 aatctgacgg tgaccgatga tggcagcacc attcagggca aagatgccga catcatgatc
     5581 atcggtggta tcccggacaa attaaaagac gataagcaga tcgacctgtt ggtgcaggcg
     5641 accgaaagct gggtgaaaac accgatgcgc cagaccccgt tccccggcat tgtgccggac
     5701 gagagcgatc gtgcggcaga aacccagtca acgctgacct cttccggtgc gatggcggcg
     5761 gtgattggct tccagtcgcc gtataacgac cagcgcagcg tgattgcgct gctggcagat
     5821 agcccacgcg gttatgaaat gcttaacgat gcggtgaacg atagcggcaa acgcgccacc
     5881 atgttcggtt cggtcgcggt gatccgcgag tccggtatca acagcctacg tgttggcgac
     5941 gtttattacg taggtcatct gccgtggttc gagcgcttgt ggtatgcgct ggcaaaccat
     6001 ccgattctgc tggcggtgct ggcggcaatc agtgtgatat tgctggcatg ggtactgtgg
     6061 cgtctgctgc gaattattag tcgtcgtcgt cttaacccgg ataacgagta attgaagatg
     6121 aatgtgttgc gtagtggaat cgtgacgatg ctgctgttgg ctgcctttag tgttcaggca
     6181 gcctgtacct ggcctgcctg ggagcagttt aaaaaggatt acatcagtca ggaagggcgc
     6241 gtcatcgccc ccagcgacgc gcgcaaaatc accacctccg aagggcaaag ttacggtatg
     6301 ttctttgccc tggcggctaa cgaccgtgta gctttcgata atattctcga ctggacgcag
     6361 aacaatctcg ctcagggctc tttaaaagaa cgtttgcccg cctggctgtg gggcaagaaa
     6421 gagaacagta agtgggaagt gctggacagc aattcggcct ccgatggtga tgtctggatg
     6481 gcctggtcgt tgctggaggc ggggcgtttg tggaaagagc agcgttatac cgacatcggc
     6541 agcgcgttgc taaaacgtat cgcgcgggag gaagtggtga cggtgcctgg gctgggttcc
     6601 atgttgttac cgggcaaagt gggttttgct gaggataaca gctggcgttt taaccccagc
     6661 tacctgccgc cgacgctggc gcagtatttc acccgctttg gcgcgccgtg gaccacgctg
     6721 cgcgaaacca atcaacgttt attgctggaa accgccccga aaggtttttc gccagactgg
     6781 gtgcgctatg agaaagacaa aggctggcag ctaaaagccg aaaaaacatt gatcagcagc
     6841 tacgacgcta tccgcgttta catgtgggta ggcatgatgc ctgacagcga tccgcaaaaa
     6901 gcgcggatgc tcaaccggtt taaaccgatg gcgacattca ctgagaaaaa cggttatccg
     6961 ccggaaaaag tggatgtggc tacggggaaa gcgcagggta aaggaccgat cggtttttct
     7021 gccgccatgc tgcccttttt acaaaaccgc gatgcgcagg ccgttcagcg ccagcgcgtg
     7081 gccgataact ttcccggcag cgatgcctat tacaactatg tgctgaccct gtttggacaa
     7141 ggctgggatc aacaccgttt ccgcttctcg acaaaaggtg agttattacc tgactggggc
     7201 caggaatgcg caaattcaca ctaaacatat tcacgctttc cctcggtctg gccgtcatgc
     7261 cgatggtcga ggcagcacca accgctcagc aacagttgct ggagcaagtt cggttaggcg
     7321 aagcgaccca tcgtgaagat ctggtgcaac agtcgttata tcggctggaa cttattgatc
     7381 cgaataaccc ggacgtcgtt gccgcccgtt tccgttcttt gttacgtcag ggcgatattg
     7441 atggcgcgca aaaacagctc gatcggctgt cgcagttagc gccgagttca aatgcgtata
     7501 aatcgtcgcg gactacgatg ctactttcca cgccggatgg tcgtcaggca ctgcaacagg
     7561 cacgattgca ggcgacgacc ggtcatgcag aagaagctgt ggcgagttac aacaaactat
     7621 tcaacggtgc gccgccggaa ggtgacattg ctgtcgagta ctggagtacg gtggcgaaaa
     7681 ttccggctcg ccgtggcgaa gcgattaatc agttaaaacg catcaatgcg gatgcaccgg
     7741 gcaatacggg cctgcaaaac aatctggcgc tattgctgtt tagtagcgat cgccgtgacg
     7801 aaggttttgc cgtcctggaa cagatggcaa aatcgaacgc cgggcgcgaa ggggcctcta
     7861 aaatctggta cgggcagatt aaagacatgc ccgtcagtga tgccagtgtg tcggcgctga
     7921 aaaaatatct ctcgatcttt agtgatggcg atagcgtggc ggctgcgcaa tcgcaactgg
     7981 cagaacagca aaaacagctg gccgatcctg ctttccgcgc tcgtgcgcaa ggtttagcgg
     8041 cggtggactc tggtatggcg ggtaaagcca ttcccgaact acaacaggcg gtgcgggcga
     8101 acccgaaaga cagtgaagct ctgggggcgc tgggccaggc gtattctcag aaaggcgatc
     8161 gcgccaatgc agtggcgaat ctggaaaaag ccctcgcact ggacccgcac agcagcaaca
     8221 acgacaaatg gaacagtctg ctgaaagtaa accgctactg gctggcgatc cagcagggcg
     8281 atgctgcgct gaaagccaat aatcctgacc gggcagaacg cctgttccag caggcgcgta
     8341 atgtcgataa caccgacagt tatgcagtgc tggggctggg cgatgtggcg atggcgcgaa
     8401 aagattatcc cgccgccgaa cgttattatc agcagacctt gcgtatggac agcggcaaca
     8461 ctaacgccgt gcgcgggctg gcaaatattt accgccagca atcgccagaa aaagctgaag
     8521 cgtttatcgc ctcgctctct gccagtcagc ggcgtagcat tgatgatatc gaacgcagcc
     8581 tgcaaaacga ccgtctggca cagcaggcag aggcactgga aaaccagggc aaatgggcgc
     8641 aggcggcagc acttcagcgg caacgactgg cgctggaccc cggcagcgta tggattactt
     8701 accgactttc gcaggatctc tggcaggccg gacaacgcag ccaggccgat acgttaatgc
     8761 gcaatctggc gcagcagaag tcgaacgacc cagagcaggt ttacgcttac gggctgtacc
     8821 tacgcttacg ggctgtacct ctctggtcat gaccaggaca gagcggcgct ggcgcatatc
     8881 aacagcctgc cgcgtgcgca gtggaacagc aatattcagg agctggttaa tcgactgcaa
     8941 agcgatcagg tgctggaaac cgctaaccgc ctgcgagaaa gcggcaaaga ggcagaagcg
     9001 gaagcgatgc tgcgccagca accaccttcc acgcgtattg acctcacgct ggctgactgg
     9061 gcgcaacaac gacgtgatta caccgccgcc cgcgctgcat atcagaatgt cctgacgcgg
     9121 gagccagcta acgccgacgc cattcttggt ctgacggaag tggatattgc tgccggtgac
     9181 aaagcggcgg cacgtagcca gctggcgaaa ctgcccgcta ccgataacgc ctcgctgaac
     9241 acacagcggc gcgtggcgct ggcacaggcg cagcttggcg ataccgcagc agcgcagcgg
     9301 acgtttaata agttgatccc gcaggcaaaa tctcagccac cgtcgatgga aagcgcgatg
     9361 gtgctgcgtg atggtgcgaa gtttgaagcg caggcgggcg atccaacgca ggcgctggaa
     9421 acctacaaag acgccatggt cgcatccggt gtgactacga cgcgtccgca ggataacgac
     9481 acctttaccc gactgacccg taacgacgag aaagatgact ggctgaaacg tggcgtgcgc
     9541 agcgatgcgg cggacctcta tcgccagcag gatcttaacg tcacccttga gcacgattac
     9601 tggggttcga gcggcaccgg tggttactcc gatctgaaag cgcacactac catgttgcag
     9661 gtggatgcgc cgtattctga cgggcggatg ttctttcgca gtgatttcgt caatatgaac
     9721 gtcggcagtt tctccactaa tgccgatggc aaatgggatg acaactgggg cacctgtaca
     9781 ttacaggact gtagcggcaa ccgcagccag tcggattccg gtgccagcgt ggcggtcggc
     9841 tggcgaaatg acgtctggag ctgggatatc ggtaccacgc cgatgggctt caacgtggtg
     9901 gatgtggtcg gcggcatcag ttacagcgat gatatcgggc cgctgggtta caccgttaac
     9961 gcccaccgtc ggcccatctc cagttctttg ctggcctttg gtgggcaaaa agactccccg
    10021 agcaataccg ggaaaaaatg gggtggcgta cgtgccgacg gtgtggggct aagtctgagc
    10081 tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta gtggcgacca gttaaccggt
    10141 aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg gctattacta taaggtcatt
    10201 aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca tgatctggca ttacgacaaa
    10261 gatctgagtg gctactcact cggtcagggc ggttactaca gtccgcagga atacctgtcg
    10321 tttgccatac cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga gctgggggcg
    10381 tctggctcgt ggtcgcattc acgcaccaaa accatgccgc gttatccgct gatgaatctg
    10441 atcccgaccg actggcagga agaagctgcg cggcaatcca acgatggcgg cagcagtcag
    10501 ggcttcggct acacggcgcg ggcattactt gaacgacgtg ttacttccaa ctggtttgtt
    10561 ggcacggcaa ttgatatcca gcaggcgaaa gattacgcac ccagccattt cctgctctac
    10621 gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca gccgctgata
    10681 ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg cgatcgggta
    10741 tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag tcgctcgtta
    10801 acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt tattttttgc
    10861 accgttttgc tgttccatct ggtccagcag aatcgctata acacggctac gcaactggaa
    10921 agcattgctc gctctgtccg cgaaccctta tcttcagcta ttttgaaagg cgatattccc
    10981 gaagcggaag ctattcttgc cagcattaaa ccggcaggcg tggtcagccg tgccgatgta
    11041 gtgctgccta accagttcca ggcgctgcgt aaaagtttta ttccagagcg cccggtgccg
    11101 ataatggtta ctcgcctgtt tgagctaccg gttcaaatct cgctgggcgt ttactcgctc
    11161 gaacgtccgg caaacccgca gccaattgcc tatctggtac tacaggcgga ttccttccgt
    11221 atgtataagt tcgtgatgag caccctctca acgttagtga ccatttactt acttttgtcg
    11281 cttatcctga ccgtcgccat cagctggtgc attaaccgcc tgattttgca tccgttacgc
    11341 aatattgctc gcgaacttaa cgccatccca gcccaggagc ttgttggtca ccaactggca
    11401 ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta caacctcaac
    11461 cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg cttcccggtg
    11521 tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggttgt cgcgcgtaaa
    11581 caaaccaccg cgctgatgat catcacctgt gaaaccctgc gtgatactgc gggcgtgctg
    11641 aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa atcggtactg
    11701 tcgccacgta tgatcctcgc gcagattagc ggttatgact ttgctgtcat tgccaacggt
    11761 gtacaggaac cgtggcacgc aatcacctta ggtcagcaag tgctcaccat catgagcgag
    11821 cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattggcgt ggcgatgttc
    11881 tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc atttaccgct
    11941 cgccataaag gcaagaatca gattcagttc tttgatccgc agcagatgga agccgcccag
    12001 aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca gtttgctatt
    12061 tggttacagc cacaggtcga gatgaccagc ggtaaactgg tcagtgcgga agtgttactg
    12121 cgtatccagc aaccggatgg cagttgggac ctgccggatg gcttaatcga tcgcattgag
    12181 tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg tcgattgctt
    12241 gcagcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc tgcgctgcaa
    12301 ctgatgcacc caaatatggt ggcggatatg ctggaactgt taacccgcta tcgcattcag
    12361 ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgaccc tcatgctgcg
    12421 gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga tgatttcggc
    12481 atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat cgacgtactg
    12541 aaaatcgaca aaatgtttgt tgaaggcttg ccggaagata gcagcatgat tgctgcaatt
    12601 atcatgctgg cgcagagcct gaacttacaa atgattgccg aaggcgtgga gactgaagca
    12661 caacgcgact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct ttttgctcac
    12721 ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct accccaaact
    12781 gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt ttaacctttt
    12841 tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat tttaaggccg
    12901 caggtacccc ataaccttac aagacctgtg gttttactaa aggacaccct atgaaaacct
    12961 ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt attctccttg
    13021 gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc ttcgttaagc
    13081 tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc attgcgggca
    13141 tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac tttgaaattg
    13201 tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag cctggtgccg
    13261 gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac gccgatcagg
    13321 cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc gtcattggcg
    13381 catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt ggttttgcgc
    13441 tccaccgtct gggcagcaaa ggccaactga tttttaacgt catcgaaagt ttctcgcagg
    13501 tcatcttcgg catcatcaat atgatcatgc gtctggcacc tattggtgcg ttcggggcaa
    13561 tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg cagctgatta
    13621 tctgtttcta tattacctgt atcctgtttg tggtgctggt attgggttca atcgctaaag
    13681 cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg ctgattgtac
    13741 tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg gagaaactcg
    13801 gctgccgcaa atcggtggtg gggctggtca tcccgacagg ctactcgttt aaccttgatg
    13861 gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact aacagtcaga
    13921 tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct aaaggggcgg
    13981 caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg gtgggccatt
    14041 tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca gaagctcgtg
    14101 cgctgactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag tgggtgaaag
    14161 aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat ggcaaaacgc
    14221 acgaattatc ctcttaatct caccacttat gcccgcactc ccttccccga gtgcgggtgt
    14281 ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag gtttatttca
    14341 cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt aacgtcattt
    14401 agactgactg acaacgggca gttttttatt accaggatag ttgatcaggg gttcacatgc
    14461 agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    14521 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    14581 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    14641 tggttaatat cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    14701 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    14761 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    14821 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc
    14881 tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa
    14941 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    15001 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    15061 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg
    15121 tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg tttggcgaac
    15181 tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag
    15241 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    15301 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    15361 gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagc aaagacatcg
    15421 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    15481 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    15541 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    15601 tgcagaaact gtttgccgcc tatgcgcggg ctgataccga tattctgatg ggtcagcgga
    15661 tgcgttcgct gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    15721 aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt cagcagttgt
    15781 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    15841 cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgctgctgcc gccacctctg
    15901 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    15961 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac
    16021 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    16081 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg
    16141 cactgccgcc cgtcaaacat accgccagat aaccggcgct gaaagagtcg ccagctgcgg
    16201 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatccact aacccttcgc
    16261 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    16321 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcat
    16381 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac acctgctgtg
    16441 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    16501 cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    16561 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    16621 ccgcagactg ctcactctcc agccagaatt tggcggcggc ttcgttccgc cagtagtaga
    16681 acgtacgctc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga cggttttcca
    16741 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atttgctggc
    16801 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    16861 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    16921 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    16981 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    17041 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    17101 ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgcccttg cgcgccgata
    17161 atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca
    17221 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    17281 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcggacggtt
    17341 aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc
    17401 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    17461 gattgatttg ctggcgcaaa aagccgactt ctttgtagag cacgacctgc tggcatcggt
    17521 caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccaaat
    17581 tgagcgtctt ccctggctgc gtttcgaact ggtggaacac atccgcctgc cgaaagattc
    17641 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gcaccgggat
    17701 ggcaaatttc tctgcgctaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagagct
    17761 gttcgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc ttttacatct
    17821 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg aagagtggcg
    17881 tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    17941 gatggaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgctgggct
    18001 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    18061 caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt
    18121 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    18181 gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaaaca
    18241 agaaactggc tggcgcagct gggtgccgtg gcctcatgta catgcagaag acatcattct
    18301 tggtaatcca ccggatattc ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac
    18361 gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggttca agctcgaaaa
    18421 gcccgacgcg cgcctgattc gcctctccga aaagaacaat aactggacgt ttaatcttgc
    18481 caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    18541 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    18601 aatttttgtt gatccgttag gcaagccgct gccgttcagc gaagttactg gatcgaaagg
    18661 taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc agggacgtta
    18721 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    18781 agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga
    18841 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    18901 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    18961 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    19021 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    19081 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    19141 cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga
    19201 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    19261 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    19321 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    19381 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcga cgaatattca
    19441 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    19501 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    19561 tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca acagtaacgg
    19621 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    19681 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    19741 ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga tttttgcttt
    19801 cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt cagaacagct
    19861 ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct
    19921 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac cgctgattgc
    19981 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccgacggcgg cgttactggc
    20041 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    20101 gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt agatttttgg
    20161 caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct
    20221 tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca tcaggctttt
    20281 cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc
    20341 aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt agtctgcaac
    20401 caggctgcga tatacggtgc aacggaagcc ccgagaatcg acgctacgtt gtaagagaat
    20461 gatgctccgg tgtaacgcac ttctgttggg aacagctctg gtaacagcgc gcccatcggc
    20521 ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg
    20581 ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg
    20641 ataattacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc gactggcacc
    20701 atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt acgcggcagg
    20761 ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt catgatgtaa
    20821 aacagcgtat aggttgccag catgatgaac gtacccagta cggttacgcg aacatgtttg
    20881 gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac
    20941 accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag taccgccgag
    21001 aagataaatg gcacacgcca gccccagctc ataaactgct cgtcggtcag tagccaggaa
    21061 agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcccag ctgtgggaag
    21121 gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg
    21181 ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagcggg
    21241 gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt tgaaatcccc
    21301 atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca
    21361 aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt
    21421 agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac aataacggcc
    21481 gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca
    21541 aggactttat tacgcgagtt gatcggcgtg tattcttgct cgtggtcgag tgttgtggct
    21601 gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa
    21661 aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga
    21721 taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg
    21781 gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg
    21841 agttggaatg aaattgtttg ctcccaaata gatggctgaa gaggctctgg ggaagagttt
    21901 caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat ccggcctacg
    21961 gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca tccggcctat
    22021 tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc
    22081 acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc agccccagca
    22141 cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg taggtcatca
    22201 cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc agggcttttt
    22261 tgcgcggacg gtggcgcggt aaccgccaga agatccagaa gaaaagcaga tagttggcga
    22321 agatggaaat cgccaggcca atcaaccgcc acgtcggttt cagccactca atgctgttca
    22381 ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg
    22441 tcacaatcag cgcaatcaac aaaccaatca gcgaaataaa atcacgtaga tattttaccc
    22501 agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg gcgcgaatcg
    22561 cttcacgcag gttacccatc cagttgatgc cggaataaag cgccacagcc aggccgacaa
    22621 gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac gtggcggcta
    22681 gcgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc aacatcggat
    22741 gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg atcatcgaca
    22801 aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg ttaaagcgtt
    22861 ctgtcgcgcg aatcaggtgc gcaataaccg gttggcgttg gactttttcg gcagtggtgg
    22921 tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc aactgcttaa
    22981 tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc gtcatccgct
    23041 cttttgtcct tctgttgtgg tcattcttaa aaagtatagt cggtcagtcc aggtactctt
    23101 tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc tcccgacgct
    23161 gcggataaac cagcgaaagg gagagcggct cggcacggta gcctggcaac acttcaataa
    23221 gctgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata atccccagac
    23281 cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg ccgccagact
    23341 taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt acacccaggt
    23401 gcggtgtata acgcaccatt gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa
    23461 agcgcgtcag atagtgcgga ctggcacagt tgaccatggt cagtttgccg aggggacggg
    23521 cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa
    23581 gaatatctac cggacggtta tggctactca gttccagctc aattcccgga tgctgataga
    23641 gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc
    23701 gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt tcgctgagat
    23761 tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg agtttgaccc
    23821 gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc tgtatctggc
    23881 gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct
    23941 ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg accgattgtt
    24001 gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct aacatctaat
    24061 gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga aaagggcgga
    24121 atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt gattcccttt
    24181 aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg ccatttgtta
    24241 ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc accaatataa
    24301 ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg cgtttagctg
    24361 taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg ccaccgccaa
    24421 atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag tctttttaac
    24481 gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt caatatttat
    24541 tcatggaatg aaaatcagtt tacagcagcg tattccagga gtgagtattc agggggccag
    24601 tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag ttatgctcga
    24661 tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc
    24721 tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac aggaagttat
    24781 tcattttaat gtgctggcca ttgtgcctcg tgattcaacc gtcgagacat ttgcgttggc
    24841 ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga cgacgccgga
    24901 aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt taaccatgtt
    24961 agcggcgggc gaatcaaata aggagatcgg cagagcgctg aatatcagta ccggaacggt
    25021 aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc
    25081 aatgatgtta aatatctcct cctg
//
LOCUS       DAETQN010000059        24837 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.059, whole genome shotgun sequence.
ACCESSION   DAETQN010000059 DAETQN010000000
VERSION     DAETQN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.059"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(29..829)
                     /locus_tag="K0W68_002354"
                     /pseudo
     CDS             complement(29..829)
                     /locus_tag="K0W68_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            1396..1761
                     /locus_tag="K0W68_002355"
     CDS             1396..1761
                     /locus_tag="K0W68_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBI3073894.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1801..2457
                     /locus_tag="K0W68_002356"
     CDS             1801..2457
                     /locus_tag="K0W68_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBI3073895.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            2524..3942
                     /gene="dcm"
                     /locus_tag="K0W68_002357"
     CDS             2524..3942
                     /gene="dcm"
                     /locus_tag="K0W68_002357"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBI3073896.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3923..4393
                     /gene="vsr"
                     /locus_tag="K0W68_002358"
     CDS             3923..4393
                     /gene="vsr"
                     /locus_tag="K0W68_002358"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBI3073897.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(4382..5303)
                     /gene="yedA"
                     /locus_tag="K0W68_002359"
                     /pseudo
     CDS             complement(4382..5303)
                     /gene="yedA"
                     /locus_tag="K0W68_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            5476..6393
                     /locus_tag="K0W68_002360"
     CDS             5476..6393
                     /locus_tag="K0W68_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBI3073898.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6472..6654
                     /locus_tag="K0W68_002361"
     CDS             6472..6654
                     /locus_tag="K0W68_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBI3073899.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6825..8519
                     /gene="yedQ"
                     /locus_tag="K0W68_002362"
     CDS             6825..8519
                     /gene="yedQ"
                     /locus_tag="K0W68_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HBI3073900.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(8516..9331)
                     /locus_tag="K0W68_002363"
     CDS             complement(8516..9331)
                     /locus_tag="K0W68_002363"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HBI3073901.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(9629..9856)
                     /gene="yodD"
                     /locus_tag="K0W68_002364"
     CDS             complement(9629..9856)
                     /gene="yodD"
                     /locus_tag="K0W68_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBI3073902.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            10019..10207
                     /gene="dsrB"
                     /locus_tag="K0W68_002365"
     CDS             10019..10207
                     /gene="dsrB"
                     /locus_tag="K0W68_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBI3073903.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(10251..10874)
                     /gene="rcsA"
                     /locus_tag="K0W68_002366"
     CDS             complement(10251..10874)
                     /gene="rcsA"
                     /locus_tag="K0W68_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBI3073904.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(11159..11944)
                     /gene="fliR"
                     /locus_tag="K0W68_002367"
     CDS             complement(11159..11944)
                     /gene="fliR"
                     /locus_tag="K0W68_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBI3073905.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11953..12222)
                     /gene="fliQ"
                     /locus_tag="K0W68_002368"
     CDS             complement(11953..12222)
                     /gene="fliQ"
                     /locus_tag="K0W68_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBI3073906.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(12232..12969)
                     /gene="fliP"
                     /locus_tag="K0W68_002369"
     CDS             complement(12232..12969)
                     /gene="fliP"
                     /locus_tag="K0W68_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBI3073907.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12969..13334)
                     /gene="fliO"
                     /locus_tag="K0W68_002370"
     CDS             complement(12969..13334)
                     /gene="fliO"
                     /locus_tag="K0W68_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBI3073908.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(13337..13750)
                     /gene="fliN"
                     /locus_tag="K0W68_002371"
     CDS             complement(13337..13750)
                     /gene="fliN"
                     /locus_tag="K0W68_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBI3073909.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(13747..14751)
                     /gene="fliM"
                     /locus_tag="K0W68_002372"
     CDS             complement(13747..14751)
                     /gene="fliM"
                     /locus_tag="K0W68_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBI3073910.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14756..15220)
                     /gene="fliL"
                     /locus_tag="K0W68_002373"
     CDS             complement(14756..15220)
                     /gene="fliL"
                     /locus_tag="K0W68_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBI3073911.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            complement(15325..16452)
                     /gene="fliK"
                     /locus_tag="K0W68_002374"
     CDS             complement(15325..16452)
                     /gene="fliK"
                     /locus_tag="K0W68_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBI3073912.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(16449..16892)
                     /gene="fliJ"
                     /locus_tag="K0W68_002375"
     CDS             complement(16449..16892)
                     /gene="fliJ"
                     /locus_tag="K0W68_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HBI3073913.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16911..18284)
                     /gene="fliI"
                     /locus_tag="K0W68_002376"
     CDS             complement(16911..18284)
                     /gene="fliI"
                     /locus_tag="K0W68_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBI3073914.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(18284..18970)
                     /gene="fliH"
                     /locus_tag="K0W68_002377"
     CDS             complement(18284..18970)
                     /gene="fliH"
                     /locus_tag="K0W68_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBI3073915.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18963..19958)
                     /gene="fliG"
                     /locus_tag="K0W68_002378"
     CDS             complement(18963..19958)
                     /gene="fliG"
                     /locus_tag="K0W68_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBI3073916.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(19951..21609)
                     /gene="fliF"
                     /locus_tag="K0W68_002379"
     CDS             complement(19951..21609)
                     /gene="fliF"
                     /locus_tag="K0W68_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBI3073917.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            21825..22139
                     /gene="fliE"
                     /locus_tag="K0W68_002380"
     CDS             21825..22139
                     /gene="fliE"
                     /locus_tag="K0W68_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBI3073918.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            22473..22805
                     /gene="emrE"
                     /locus_tag="K0W68_002381"
     CDS             22473..22805
                     /gene="emrE"
                     /locus_tag="K0W68_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBI3073919.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            22974..23525
                     /gene="ybcL"
                     /locus_tag="K0W68_002382"
     CDS             22974..23525
                     /gene="ybcL"
                     /locus_tag="K0W68_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBI3073920.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            23535..24332
                     /locus_tag="K0W68_002383"
     CDS             23535..24332
                     /locus_tag="K0W68_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3073921.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT         6112 a         6136 c         6351 g         6238 t
ORIGIN      
        1 actaaatcag taagttggca gcatcaccca ttgcaagata aacattgttg gcgtcgtatt
       61 ttgcaccaaa tgtccatgct tcagctgtat caccaccagc aaatttgctg tattcattgt
      121 agtgataccc gccaagagct acttgagaat tggtacggtc agagtttgag taagcggcac
      181 ctaagctcaa accgaagtca aagtcatatg aagtggaaag accaaaacca tcaccgttct
      241 ggaagcgaac gtcttcgtga ccatttttag taccttcttg atcttctcct gcattgctgc
      301 cttcgttgtt accttgatac tgcaatgcaa agttcagacc ttccaccaga ccgaagaaat
      361 cactattgcg gtaagtagct accccatttg cacggcctgc catgaagtta tcagtccagg
      421 tataggagtc gccgccgaat acaggaagca tatctgtcca ggcttcaacg tcgtaaatca
      481 caccatagtt acgaccatag tcaaaggagc ccatctcgcc gaacttcaga cctgcaaaag
      541 caagtttgac ataagtatta tcagaacctt cagtgtcgct agcctttgtt ttggtcagtg
      601 cttgaccata accagttaac tgatctgtaa tttgggtctc acctttgaag cctaactcga
      661 tgatagtttc atcgccgtca tcgccagggt tatcggaaaa agtatgacga gcatcaactt
      721 ttccgtacag atccagttta ttgccatttt tattataaat ttcagccgca tttgctgcgc
      781 cagcaactaa taacgctggg accagcattg ccagaacttt tcttttcatt atgtattccc
      841 ttgtgattat aatcttcatg aatatatcaa taagtgccgt tatccaaaaa aaaagcacat
      901 ttggatacta ttctatgaag ttcattttat tttaaagatt gcgtgtaaca aatgtattta
      961 aaagatttca aaatgtttct tatctatttg atataattca gaattatgaa ttctaaatat
     1021 aaacatccat gaaaattata aatgtatatc aatatgttat gagttgaatg gaaagatagg
     1081 gagtatcaat gattgttgcg ccaaacggca ggcgtgatca cgcgcacatt aatgaaggta
     1141 taaaaaaagc cggcatcatg ccggcccaaa aaatataaac aagacgataa aaggctaata
     1201 acggggttat catgacacag aaatcaaaaa aggtataatt gtattcagtg ctaatatatt
     1261 gtttgggtga attaataaaa ctattaccaa ggatataaat aaatctttat tgctgttttt
     1321 ccagtaatta ttgccgttgc gcaaaattta tctatttgtt caaaaaatga ttgagtcttg
     1381 actggctcat ccaatgtgga aaaatgtgac ttttatcaca taatagtact aagtctgaat
     1441 tttccgggtt atctcaaaat ggaatacggt tcgacaaaga tggaagagag actctctcgc
     1501 agccctggcg gcaaattggc tttgtgggct ttctatacat ggtgtggcta tttcgtctgg
     1561 gcaatggcgc gttacatctg ggtgatgagc cggatccctg atgccccggt gtcgggattt
     1621 gaaagcgatt taggctctac cgcgggaaag tggttagggg cactggtcgg atttttattt
     1681 atggctctgg tcggggcatt gttaggcagc attgcctggt acacccgacc tcgtcccgca
     1741 cgtagcagac gctatgaata aaaactgcta aggtggcgac tctttggcaa cggagcgcgt
     1801 atggacttac aacactggca ggcacagttc gaaaactggt tgaaaaacca tcatcagcac
     1861 caggacgcgg cacagaagct ggcggcagat gacgatgtcg atatgctggt gattttaacc
     1921 gcctgttatt ttcacgatat tgtgagcctg gcgaaaaatc atccgcaaag gcagcgttct
     1981 tcaatcctgg cggcagaaga gacgcgtcgc ctgctccgtg aagagttcga gcaatttccg
     2041 gcggagaaaa tcgaggccgt ttgtcatgcc attgccgctc acagtttcag cgcgcaaatc
     2101 gcccccttaa caacggaagc taaaatcgtc caggatgcgg atcggctgga agccctgggg
     2161 gcgattggcc tggcgcgcgt gtttgccgtt tcaggggcat tgggcgtggc gctgtttgat
     2221 ggtgaagatc cgttcgcaca gcatcgcccg cttgatgata aacgctacgc gctggatcat
     2281 ttccagacta agttgctgaa gctaccgcaa accatgcaaa ccgcaagagg caagcagctg
     2341 gcgcagcaca acgcgcattt tttagtcgag tttatggcga agctcagtgc cgaactggcg
     2401 ggggagaatg aaggtgtcga tcacaaggtg atagatgcgt tttcacccgc gggataagcg
     2461 cgtggcccta aatggctgta attatgttaa cctgtcggcc atctcagatg gccggttaaa
     2521 tctatgcagg aaaatatatc agtaaccgat tcatacagca ccgggaatgc cgcacaggca
     2581 atgctggaga aactgctgca aatttatgat gttaaaacgc tggtggcgca gcttaatggt
     2641 gtgggtgaga atcactggag cgcggcaatt ttaaaacgtg cgctggcgaa tgactcggca
     2701 tggcaccgtt taagtgagaa agagttcgcc catctgcaaa cgttgttacc caaaccatcg
     2761 gcacatcatc cgcattatgc gtttcgcttt atcgatctat ttgccggaat tggcggcatc
     2821 cgtcgcggtt ttgaatcgat tggcggacaa tgcgtgttta ccagcgaatg gaacaaacat
     2881 gcggtacgca cttataaagc caaccattat tgcgatccgg cgacgcatca ttttaatgaa
     2941 gatatccgcg acatcaccct cagccataaa gaaggcgtga gtgatgaggc ggcggcggaa
     3001 catattcgtc aacacattcc tgaacacgat gttttactgg ccggtttccc ttgtcagcca
     3061 ttttcgctgg ctggcgtatc gaaaaagaac tcgctcgggc gggcgcacgg ttttgcctgc
     3121 gatacccagg gcacgctgtt ttttgatgtg gtacgcatta tcgacgcgcg tcgtccggcg
     3181 atgtttgtgc tcgaaaacgt caaaaacctg aaaagtcacg accagggtaa aacgttccgc
     3241 atcatcatgc agacgctgga cgaactgggc tatgacgtgg ctgatgcaga agataacggg
     3301 ccggacgatc cgaaaatcat cgatggtaaa cattttctgc cgcagcaccg tgaacgcatc
     3361 gtgctggtgg gttttcgtcg cgatcttaat ctgaaagccg attttactct gcgtgatatc
     3421 agcgaatgtt tccctgcaca gcgagtgacg ctggcgcagc tgttggaccc gatggtcgag
     3481 gcgaaatata tcctgacgcc ggtgctgtgg aagtacctct atcgatatgc gaaaaaacat
     3541 caggcgcgcg gtaacggctt cggttatgga atggtttatc cgaacaatcc gcaaagcgtc
     3601 acgcgtacgc tgtctgcgcg ttattacaaa gatggcgcgg aaattttaat cgatcgcggc
     3661 tgggatatgg ccacgggtga gaaagatttt gacgatccgc tgaatcagca acatcgtcca
     3721 cgtcggttaa cgcctcggga atgcgcgcgc ttaatgggtt ttgaagcgcc gggagaagcg
     3781 aaattccgca ttccggtttc ggacactcag gcctatcgcc agttcggtaa ctcggtggtc
     3841 gtgccggtct ttgccgcggt ggcaaaactg cttgagccaa aaatcaaaca ggcggtggcg
     3901 ttgcgtcagc aagaggcaca acatggccga cgttcacgat aaggccactc gcagcaaaaa
     3961 tatgcgcgcg attgccacgc gtgatacggc gattgagaag cgcctcgcca gtctgttaac
     4021 cgggcagggc ctggcgtttc gcgttcagga cgccagtctg cccggaagcc cggattttgt
     4081 cgttgatgaa tatcgctgcg tgatatttac ccatggctgc ttctggcatc atcatcactg
     4141 ctatctgttt aaagtgcctg cgactcgaac cgagttctgg ctggagaaga taggtaaaaa
     4201 tgttgaacgc gatcgccgcg atatcagtcg tttgcaggaa ctcggctggc gcgtactgat
     4261 tgtctgggag tgtgcattac gtgggcgcga gaagctgacg gatgaagcgc ttaccgagcg
     4321 tctggaagag tggatctgcg gcgaaggtgc cagcgcgcag atcgacacgc aggggattca
     4381 tttactcgct tgatgcgtcc tgaataactg gcgcaactac gggttttgcc gggaagagat
     4441 attttcccaa cgtgaccagt accaccgcga agacaattac gccgagcgcc agccattcaa
     4501 tcttcgacag cgtctctccg cccagtcccg tacccagcaa gaccgcgacc accgggttaa
     4561 cgtaggcgta gctggtggcg agagccggac tgacattacg gattaaatac atataagcgt
     4621 tgatggcgat aatcgaacca aacagcgcca gatagccgac cgcaaggaag cctgaaaggg
     4681 aagggagcgc cgtcagtttt tcacctgcaa tcatcgacgc gatcattaac accacgcctg
     4741 ccgccagcat ctcaatcgca cccgccatca tccctacagg taaggtaatg cgcgagccat
     4801 aaaccgagcc aaacgcccag ctaatcgagc cgattaaaat cagaatcgcg ccccacggat
     4861 tgccgcttaa atttccaccg ctattgagca tgatgattcc ggcaagccca atggcgatac
     4921 ccacccattc cagtttgcgc gttttaatgc caaacaggcg gctgaagcac cagggtaaag
     4981 aggggcacgg ttgcaaccac aacggcggcg atgccggaag gaacattttg atgctcggca
     5041 accgtcacca tgccattacc gacagccagc aataacaggc caatcagcgc ggcattgagc
     5101 agcggacgta gcggggggag cttgtgtccg cgcagtagca aaaatgccat caataaaata
     5161 ccggctgcca ggaatcgaac gcctgccatc attaacggag gccagctttc cacgccaatc
     5221 cgaatgacaa aataggttga gccccaaatg atatacaacg caaacagcgc gccaaaaagc
     5281 ggtaacaact ggcggaaacg cataatccct cacggtggaa ataaaaaggt ggttcatagt
     5341 aaacgtgaaa atcattctgc tggcgagaga tataattgca cttgattgta aaaaaaatgt
     5401 tgacctgtga agcaagtatc agagctgccg tttttgcttc atacttacac ccttcaacaa
     5461 taaaaatgag agggaatgct tttggccggg agtagtttac tgacgttgct cgatgatatc
     5521 gccacactac tggacgatat ctccgtgatg ggcaaactgg cggcgaagaa aaccgccggt
     5581 gtattagggg atgacttatc gctcaatgcg caacaagttt caggcgtgcg ggccaaccgg
     5641 gaacttcccg tggtctgggg cgtggcgaaa ggatcgctga ttaataaagt gattctggtg
     5701 ccgctggcgc tgatcatcag tgcgtttatc ccgtgggcga ttacgcctct gttgatgatt
     5761 ggtggcgcgt ttctctgctt tgaaggagta gagaaagtgc tgcatatgct ggaggcgcgt
     5821 aaacataaag aagatccggc gcagagccag cagcgtctgg agaagctggc ggcgcaggat
     5881 ccgctgaagt ttgaaaagga caaaataaaa ggggcgattc gtaccgattt tatattgtct
     5941 gccgaaatcg tcgccatcac gctggggatt gtggccgaag cgccgttgct taatcaggtg
     6001 ctggtgcttt caggcatcgc gctggtagtg accgtgggcg tctacggtct ggtaggggtt
     6061 atcgttaaga ttgatgacct ggggtattgg ctggcggaaa aatccagcgc gctgatgcag
     6121 gcattaggta aagggttatt gatcattgcg ccctggttga tgaaggcgtt atcgattgtc
     6181 ggcacgctgg cgatgttcct cgtcggcggc ggtattgtgg tacatggtat tgcgccgctg
     6241 catcacgcca ttgaacattt cgccgggcag caaagtgcag tggtggcgat gatattaccg
     6301 actgttttaa atctgattct tggatttatc atcggcggca tcgtggtgct gggagtgaaa
     6361 gccgtagcga aaatgcgcgg tcaggcacat taatactcgt acaggctatt atgcaaaatt
     6421 cgccatcttc aactaaggtg aacaagtttc actcgaaaaa aggaggcgag tatgagcttt
     6481 atggttagtg aggaagttac ggttaaagag ggcggcccgc ggatgattgt cactggatac
     6541 tccagcggta tggttgagtg tcgttggtat gacggttacg gggtcaagcg ggaagctttt
     6601 catgaaaccg agcttgttcc gggggagggg agtcgttctg cggaagaagt ttgaaagggt
     6661 atcattaaca ccaggccgga tcacttccgg cctggtgtgc caatgcctgc aatgaatgat
     6721 agtcgcgtct tatcatgcca ccattataaa attatcgctg ttttgcggta cgctatcgcc
     6781 aggttatgcc agaatcataa aaaagcaggt tgggagtcgt cagggtgcag cacgagacaa
     6841 aaatggaaaa ccagagctgg ttgaaaaaac tcgcacgccg cctggggcct ggtcatgtcg
     6901 ttaatctctg ctttatcgtg gtattgcttt tttccacctt gctgacctgg cgtgaagtgg
     6961 tagtgctgga agatgcctat atctccagcc agcgtaatca tctggaaaac gtagccaacg
     7021 cgctcgataa gcatttgcag tataacgtcg acaaactgat ctttttgcgt aatggcatgc
     7081 gcgaagctct cgtagcgcca ctggatttca cttcactgcg taatgctgta accgagttcg
     7141 aacagcatcg cgacgagcac gcctggcaaa ttgaactcaa ccgacgacgc accctgtcag
     7201 tcaatggcgt atcggatgca ttagtcagcg aggggaatct cctgtctcgc gaaaatgaaa
     7261 gcctcgacaa tgaaattacc gctgcactgg aagttggtta cttgctgcga ctggcgcaca
     7321 acacctcgtc gatggttgaa caggcgatgt atgtctcgcg tgccggattt tacgtttcga
     7381 cgcagccgac cttgtttacg cgcaatgtac caacgcgtta ttacggctat gtcacccaac
     7441 cctggtttat cggccattcg caacgagaaa atcgtcaccg tgcggtacgc tggtttactt
     7501 cgcaaccgga acacgccagc aatactgaac cgcaggttac cgtcagtgtt ccggtagaca
     7561 gtaataacta ctggtatggc gtgctgggga tgagtattcc cgtgcgtacc atgcagcaat
     7621 ttttaagaaa cgccatcgat aaaaacctcg atggtgagta tcagctctat gacagtaagc
     7681 tgagattttt gacctcttcc aatcctgacc atccaacagg gaatattttt gatcctcgtg
     7741 aactggcctt gctggcgcag gcgatggaac atgacacgcg gggcggcatt cgtatggaca
     7801 gtcgctatgt tagctgggaa cgtctggacc atttcgacgg tgtgctggtg cgcgtccata
     7861 cgctaagcga aggcgtgcgc ggcgatttcg gcagtatcag cattgcatta accctgctgt
     7921 gggcgctctt taccaccatg ttactcatct cctggtatgt gattcgccgg atggttagca
     7981 acatgtatgt tctgcaaagc tcgttgcagt ggcaggcgtg gcacgacacc ttaacccgtt
     8041 tatataatcg tggcgcactg tttgaaaaag cccgtccgct cgcgaaattg tgtcagacgc
     8101 accaacatcc tttttctgtc attcaggtcg accttgacca ttttaaagcg attaatgacc
     8161 gctttggtca tcaggcgggc gaccgtgttc tttctcatgc tgccggatta attagcagtt
     8221 ccttgcgtgc gcaggacgtt gccgggcggg tcggtggtga ggagttttgt gtgattctgc
     8281 caggcgcgag tctgacggag gctgcggaag tcgcagaacg tattcgcctg aagttaaatg
     8341 aaaaagagat gttgattgct aagagtacga cgatacgcat cagtgcctcg ttgggggtaa
     8401 gtagcagcga ggaaaccggt gattatgatt ttgaacaact ccagtcactg gctgaccgtc
     8461 ggctttatct cgctaaacag gccgggcgta atcgggtatg cgcgagcgat aacgcttaac
     8521 gagcggagaa aaaatggtcc agcccttcac gccagccttc cggtccttca cgctgcgttc
     8581 gccagacgcg ggtcggatcc tcatcatgca gatgcacccc ttcacggttt agccctttca
     8641 caatcaccgc gtaatccatt acctccagta agggcgcatc gtttggccca tcgcccaggc
     8701 caagtgtggt tgggcgtttg cctgataatt gttgataggt cgcgataatc cagttggcgg
     8761 cctgatcttt tccggcagag gcatccagga cgtgccagaa gcgcgcaccc tgcataaact
     8821 gtaagcccag ttcgttcaga cgagcggtaa attgtgccat acgctcgtca ctgtcgcgcc
     8881 agattagcgt taccgacgcc tcatgcagct gcgtcagcgc cgcctggcta cggcttaatc
     8941 ccgtccattc ggcgatggtt gcatcgtcga catcatcgaa agtcgtgaat ttaaaatgtt
     9001 ctttctcgcg tagcgtattt aaaacctggc tgatttcgcc atggctaata cctgagatga
     9061 tgcgtggaaa accgtctata tcctgccatt gttcagcaag ctggatcact gcgccatttt
     9121 cggcaatcag tggtaaccct tgtagcccca acgttttttg caagtacagc atttccgctg
     9181 aagttttact gctacagaga atgacgggaa cattcgcttc gcgtaaacgg ctgagccaga
     9241 gggcagtcgg ttgccagtca taactatgac tgtccagcag ggtgccatca agatcgctaa
     9301 aaaccagtag tggttgttga attgaaaaca tggtcgtgca gttctcccct taccaggatg
     9361 attctcaaaa cagcacaaat aatcaaagat gattttttcg ggagcgatcc cgttgacgcc
     9421 agagccacca aggggtgacg cacgtcacat ttctattcat aagtagcgtt aattattcat
     9481 atcgcgaata ttttcttgtc agcgaaaaaa attgcggata aggtgatgaa cacatcagat
     9541 ttcctggtgt aacgaatttt ttaagtgctt cttgcttaag caagtttcat cccgaccccc
     9601 tcagggtcgg gattttttta ttgtgcattc aacgattcac ttcagagacg ctgaagtcat
     9661 gaatatccag ttcgaaggca tccgccaatt cacgccatgt atgatattca cgtccatcga
     9721 cgctaacgtg ttcagaatcg ttctggctgc gatgaacttc gctttcgcta atttcattga
     9781 tcgccgccag cagggcatcg acatcgacgc tgacctcacg ttttgcggta tcgctgtact
     9841 cttttgcggt tttcatcatc tttcctcgca accgttactc tgttgttgcc gccagtcgtt
     9901 aagtccgcct ggcatctctt aagtatagac cgtcgagaaa atcagcactc gcgcgcgcct
     9961 ggagctgcca gaaaaacgaa attgttctac actggcacaa agccacagga ggaaaacgat
    10021 gaaggtgaat gatcgggtaa cagtcaaaac ggatggcggt ccgcgtcgtc ctggcgtggt
    10081 actggcagtt gaggagttta gtgaaggcac aatgtacctg gtttcgctgg aagactaccc
    10141 gctcggcatc tggttcttta atgaagcagg gcatcaggac ggtatctttg tggagaaagc
    10201 agagtaatct tattcagcct gactggtggg aaaccaccag tcagaatgtg ttagcgcatg
    10261 ttgacaaaaa taccattagt cacattatcc gtcagtcgga cgacatggta gataacctgt
    10321 ttattatgtg ttttgatctt acgtttaata ttacctttat gcgatgaaac ggtcttggct
    10381 ttgatattca tttggtcaga gatttgaatg gttccctgac ctgccatcca cattcgcaac
    10441 atactcgatt cggttcggct caatgataac gtcggcatat ttaaaaaaga ggttatcgtt
    10501 gtctcttttt tcagaatatc gccaaggata tcgtcgagag attccggttt aatcgattta
    10561 gaacttatca ataatttttt tctgaccaat agatattcat caaaatgaac attggcaatt
    10621 gccataaaaa cgataaataa cgtattggga tgttgattaa tgatgtgctt gatgtgctga
    10681 ctgttagaag catcatggat gaaacagtcc tcattaataa acaccactga agggcgctgt
    10741 gaatcacaag ctatggcaag gtcatcaacg gtttcaatgt cgttgatttc tcttttttta
    10801 acccctctac tcaacagata cccggttaaa cctagtcggg tgtaactaca taaatccata
    10861 ataatcgttg acatggcata ccctcactca atgcgtaacg ataattcccc ttacctgaat
    10921 atttcatcat gactaaacgg aacaacatgg gtcacctaat gcgccactct cgcgattttt
    10981 caggcggact tactatcccg taaagtgttg tataatttgc ctggaattgt cttaaagtaa
    11041 atgttgcgat atgtgagtga gcttaaaaca aatatttcgc cgcaggagta tcctggaaga
    11101 tgttcgtagg tattattttt cggataatcc ttaggataac atgataaacg ttacggaatt
    11161 atattaatgg caattcacta ataatatcag ccagcaaatt aaacatctca ctgaataaat
    11221 gttcgcaaaa aggtgcaatt aacggcatta atgccgccat taaagagata ccgacagtca
    11281 gagttaatgg aaatccaata acaaaaatgg ataattgcgg ggccatacga ttcagtaaac
    11341 ctaatgccag attcagggtc agcagcagag taatgagcgg taacgccagc atcagcccgt
    11401 taaggaaaat caaactcccc gctttggtga gtgccagaaa cgcattgctg ttcaacggtt
    11461 cgccaccaat tggcagggtg tgaaaggtat cgaccagcag tgaaatcaac cataaatgac
    11521 cgttaaatgt caggaacagc agtaacgcca gcatatccat gatacgcgct aaaacgggca
    11581 tattgagatg gctggccgga tcgacaaacg tcgcaaatga cagccccatt tgcagaccga
    11641 taatttcgcc agcggttcgc acagcggcaa aggcaaattg catggtaaaa ccaagcgcaa
    11701 tgccgatcag gatctgctgc acggccagcc acagagcaaa gaacgaaaaa acaggaacat
    11761 cgttggcagg taatgatggg gcaatgacga acgtgatcat cattgccagc cccagtttta
    11821 cccgtttcgg cacgctgcgt tcgctcagaa ttggtgcggt agaaatgagc gccagcacgc
    11881 gcagtaacgg ccagaagtac aggcttaacc aggaaagcca ttgatcgctt gtcacctgca
    11941 tcattgtacg gcctacccga tgatatacgg caggttagtg aacaaggtgc ggacgtaatc
    12001 cagcaacaga ttgagcatcc acggtccggc aataataatg gcaataaata cggcgatgat
    12061 ttttggaata aacgacagcg tcatttcgtt gatctgcgtg gcggcctgca aaatactgat
    12121 gataaggccc gtgaccagcg ccaccagcaa cagcggggca gccagtgcca gcgcgacttt
    12181 catcgcttca gtccccatca tcatgaccga ttcaggtgtc attttgcctc tctagctgta
    12241 aaagctctgc gccagcgaac cgaccagcaa ttgccagcca tccaccagta caaacagcat
    12301 cagtttaaag ggcagagcaa tggtggcggg ggggaccatc atcatcccca atgccatcaa
    12361 cacgctggca atcaccaggt cgataatcaa aaaaggaatg aaaatcgtga agcctatctg
    12421 aaatgcggtt ttcaattcgc tggtcacgta ggccgggagc aaaatgcgca tcggcacggc
    12481 ttcaggtccc tgcaacgggc cggtattcgc tagtctggca aacaacccta aatctgcctc
    12541 acgggtctga cgcagcataa actcacgtag cggctgcgcc cctttttcca gcgcctcctg
    12601 cattgatatt ttctcttcgc tgaatggctg gtacgcatcg acataaattt tgtcgatcac
    12661 cgatgacata ataaaaaagg tcaaaaatag tgccagcccc agcaatacct ggttaggtgg
    12721 cgcggagggc gttcccagcg cgttacgcaa taaaccaaaa acaatgatga tgcgggtgaa
    12781 actggtcatc atcagtaaaa ttgccggaat aaacgtcaac gaggtgatga acaccagcgt
    12841 ctgcaccggg agcgaccagc tttgtccacc gccaggcagc ggctggctgg tgatacccgg
    12901 cagttgcgcg aaggcgaggg gcgtaaccag ccagagaagg acaggtgcga cagacaacaa
    12961 acgacgcatc aggatctccc actacgctta agcaaatttt tcatgaccga ctgaaaatcg
    13021 gtctgcggta tctcttctgt tggtgctgaa gggggaagtt tatgcagcag attgatttgc
    13081 cctgcggtaa cgccgagcac cagccgtgca tcttccacat cgaccaccac aacccgttca
    13141 cgcgcgccca gcgaagcact ggcgctaatt ttcagaccgt taacgccagt gcgtttcggg
    13201 gcaaatccca accgttttac cagccaggca gcagcgagga tcagggcaat aatggcgatg
    13261 agtgcgccgc tgacctgcag cagtggcgca gcagaaaccg gcgtggaaga ttgcacagta
    13321 gcgtggttat tcatcactaa cggctcaggc gacgcattcg ctcagacgga gtaatgatat
    13381 cggtgatccg cacgccatat ttatcggcaa cgaccaccac ttcgccctgg gagattaaat
    13441 aaccgttgat cagaatatcc agtggttcgc ccgccagacc gtccagcgcc acgacggacc
    13501 cttgcgtcag acgcaacagc tctttgatgg tcatccgcgt acggcccagc tcgacggtca
    13561 gcttgaccgg aatatccatt atcaggtcga tatcctgcaa cgttccgctg acatcaccac
    13621 cgccaaattg ctggaacacc gcgtcggcag cgcttttgct gctggttgat ttttgttcgc
    13681 tcaacgcttc agcccacaga tcgtccattg cgccgttgtt gtcatcggcc ggattattca
    13741 tgtcactcat ttgggctgtt cctcgttcag agaatttaaa atcgggttaa tcaaatgttc
    13801 tatccgtaac gcatactgac cgttgagagt gccgtactga ctggtcagca ccgggacgcc
    13861 gtcaacatgg gcgatgatgc catcgggttt ttctatcggc aggacgtcgc cgggtttcag
    13921 ttttaaaatc tgcgacaggc gtagcgagat atcggcaaag ttggcgacca gctccagctg
    13981 tgaatgctgc acctggcgca ccaggttatc gcgccagttc tgatcttcat tacgcgagtt
    14041 ttccagcggc gggttaacca acaattcccg tagcggctcg atcatgctga atggcagaca
    14101 gatattaaat tcgccggtca ggttgccaat ctccacatgg aacggcgtgt taaccacaat
    14161 gtcgttcggc gaggtggtga tattggtaaa tttcacctgc atttccgaac gcacgtactc
    14221 aacttccagc ggattaatcg ccttccaggc gtcgctatag ccttcaagcg ccagctttaa
    14281 catgcggttg atgacgcgct gttcggtatg ggtaaactcg cgaccttcca ctttggtcgg
    14341 gaagcgtcca ttgccgccaa acaggttatc cacggcgata aacaccagac tcggtgaaaa
    14401 caccaccagc cctgtgccgc gcagcggttt cagatggata aggttcaggt tggtcggcac
    14461 cggcaggttg cgggcaaatt catggtacgg ctgaatgcgg atggccccga cggttatatc
    14521 cgggctacga cgcagcaggt tgaacagccc catacgaaaa tggcgggcaa agcgctcatt
    14581 aatgatttcc agcgcctgca aacgttcgcg cacaacccgt cgttgggtat tcggatcgta
    14641 cggacgaatg tcactttcgc cgctaacact ggctgtcggt tcgtctttga cttcgctgtc
    14701 accattcaac agcgcatcaa tttcagcttg agaaagaata ctatcgccca tgtcgttatc
    14761 gcagaataaa agcggtatac atcacgtcgg tgacatcctg tttcggttgc ccggcaacaa
    14821 gcggggtgga aagcgtggtt ttaatctcgg caatcaggtt tttcttgcct tcttctgtcg
    14881 ccagtacggc agcatcctga cgcgaaaaca gtaacagcaa acggctacgg acttccggca
    14941 aatactcact cagccgcgag cgggtagctt catctttgag tcgcagggtt ataccgatat
    15001 aaagcacgcg atccgcatcg cccaaattga ccgtaaaggt atccagcgca tagaagaccg
    15061 gtgagggcac gacgcgatgc tgcgctttgt cttcagcggc aacctgatgc gaatgccagt
    15121 agctgtaacc tgcgctggca caggccgcga gggtaatgaa taccagaatc gggagccaaa
    15181 gcgatcgctt gcttttcttg cttatcgcgt aatcagtcat gtgttgcggt cttcctgtgt
    15241 cgctactgct tatcggctaa ttatcctgcg tcttgagcgc aacaaagcgg ggaaaagacg
    15301 cggattacgg tgctacctct gacgttaggc gaaaatatca acgccgctgt tgcctgttac
    15361 gcgcccttgt aaagagacgg gaaccggaag cgtatcgtct tcttcccccg ccagaggttc
    15421 atggtttact gtgcgttggc tttgctgttg ttgggaagcg gcctgctgct gaccactaaa
    15481 gctttcgcca ctgatgttgc tttgccctaa ctgaatgcca ctctcggcca gctgcgtacg
    15541 cagtaccggc agcgctgctt ccagggcggc gcgtacgtgc tgatgcggcg aaaccatctg
    15601 gatttgcgcc tggttatcat ccactttgag ggagatttgc acttcaccta aatcctgcgg
    15661 gtgcagacgc aactctgcac tttgttgtcc ctggcgggtg aacagcgaaa tatgctggct
    15721 taatgattgt tgccattcgt gagaacccag cggtgcactc aagacaggcg cggcgacggt
    15781 gagcagtggc tgtgtctggt gtggagtgat tagcgggctg gcggcagcgg tcaccggtga
    15841 aggtgtgctg atgacttccg ctttactctg ggcttctgct accagcggtg ttaatggctg
    15901 agctggcgtg ccgggggcat catcaggctg tgctgttgtg agttgcgcag aagtcagttt
    15961 tgtgaagagc gttggtttct ctgccggtaa cacggttgac ggcgcatcag tcactttggg
    16021 cgtattgtca aaacccggca acatcgcaaa aagggcgctc aggcttgcgg tgaggtcttc
    16081 attcagatca tccgcttttt cgtcttttgt cgtatttttg tcagccaccg cagccaacgt
    16141 catcgtatga gcggtggtta acggtgtcga tgtggattgt tcgacgttga tgacaggcag
    16201 tgtttcatcc acagggatca gtaaatccgc ttgttgcgcg tcggaaacaa tatcgctgat
    16261 cagcagctcg ccttttgtcg tgggcttatc tgttgccacc agcacctggg gggccgcttt
    16321 gtcggtagtt gtctcgcttg ctaatgcttc gctcaacaac gcgagaaaat cttgtgcagc
    16381 atcgctggct ttgccgccag gcaatgtggt ggtgtcaacg tcggcggtaa tcaagggcgc
    16441 taagcgaatc attcaggttt cctcatggcg gcgcgctggg cgaactcatc catctttttc
    16501 tgatcgaggc ggttttctgc aagcagtgcc gccgtggatt gccgttcctg cagtgtctgc
    16561 caggcctgca aacgttgttt cttttctcgc cagctgttca gggcaatgtc aactttctgc
    16621 gtccactgat taagttgctg gcgatgctga gtaatggctt tttccagcgt ctggataaac
    16681 tgctgatagt tgatccagcg gttgctggtc atcccggcac tcatatcgct gttgaggtta
    16741 ttgcgatatt cattctgata atcgatcagc attttgagct gttcttccgc ctgctgacac
    16801 ccgcgacgca tttcacccag caggcgcgcg gcatcctcta cctctttttc tgccagatct
    16861 ttcagggtcg ccagcgcacc atgttctgcc atctaccgtt atctcctggg ttatgacact
    16921 gtcgggaaaa tacgctccag cccctggaga gacgcttccc agtccgcacg ttcaaaaatg
    16981 ccttgttgta aatagccctc cagctgcggc cacagggcga tggctttatc gagcatcgga
    17041 tcgctgcctt tggcatacgc gccgacgcta accagatcgc ggttacgctg aaaactcgac
    17101 aacagctgtt tgaaggtgcg cactcgcgcg taatgttgct cactgatcaa cgccgtcatt
    17161 gcgcggctga tcgacgcttc aatatcgata gccggatagt gcccggcttc cgccagtcgg
    17221 cgagacagca caatgtgacc gtcgaggatc gcccgtgcgg agtctgcaat ggggtcctgc
    17281 tggtcatcgc cttcagtaag cacggtataa aacgcggtaa tcgagccgcc gccgctaatg
    17341 ccatttccgg cacgctcgac cagtgccggt aatttggcaa acaccgacgg tggataacct
    17401 ttggtggcgg gtggttcgcc aatcgccagc gcaatttcac gctgggccat cgcgtagcgg
    17461 gtgagggagt ccataatcag caacacatgc tgaccacgat cgcgaaaatc ttcggcaatg
    17521 cgcgtggcat aggcggcacc ttgcattcgc agaagcggag agacatccgc cggagcggca
    17581 atcaccacgg agcgtgcacg cccttcagca ccgaggatat tctcaataaa atcttttact
    17641 tcgcgcccac gttcaccaat caaacccacg acaatgacat cggcgcgggt gtaacgtgcc
    17701 atcatcccca gcagcacact tttaccaacg ccggacccgg caaacagccc catacgctgc
    17761 ccacgcccaa cggtaagcag ggcattgatt gggcgcacgc cggtgtccag cacatgttca
    17821 atcggtgtac gttgcaacgg gttaaatggc ggggtaatca gcgcaccggt ttccgtcgta
    17881 tcgggggagg gcaggccatc gagcggctta ccgctgccgt ccagaacgcg acctaataac
    17941 gccggaccga gcggcaactg cttgccgctt tgcagtcctt cagccgaaat gtttttggca
    18001 taaacccgcg cgccgggcag gacaccttcc acttcctcca gcggcattaa aaacagccgt
    18061 tgaccgttaa agccaacgac ttcgctttct acttcgtgcg tttcgctgcc gttctggcgt
    18121 tcaatgacac aggttgcgcc gagcggcaat tgtaatccgg tggcttccag caccagcccg
    18181 gtagcgcggg ttaatcgccc atagcgacgt accgcaggca actgcgccat tttggcttca
    18241 aagttatcca gcgtggttag ccagcgagtc aggcgcgtgg tcattacacc actcctggtg
    18301 ctgccagacg gcagagttct tgccagcgag tggcgacact ggcgtcgaga tcgccttcat
    18361 cggcggagac tttacagccg ccaggatgga gtgtgggatc gccccgcaag cgccagccat
    18421 gcaaacttaa ggttgcgccg agcatatcat ccacacgttg cagatcatcc gggtgcacgc
    18481 gcagctgtgg tttaccgctg aataacggtt cttgctgcaa caactgttgg atctgtttga
    18541 tcagtgccga gttatccact gttggcgtct gaccgatcac ctgacgtgcc gcctccagcg
    18601 ccatctgcat caggcgcgaa gcaatcacac tatcaagcgc atcaagggta gtttgaaatt
    18661 cgctgaccag ttgctgcatc cgggcatgaa ttggcgcttg ttgagacttc gcctctgcca
    18721 gaccttgctc cagcccctgg gccagtcctt cctgatagcc ctgctcatga ccttgctggc
    18781 gaccttcggc aatccccgcc tgataacctt gctcatgggc ctgcatttgc agttgcgcca
    18841 gttgctgctc aaggctaggt tcggcctctt caatgatggt ttcttccggc tcgaccatgg
    18901 gcacaaactc tgcctgtggt ggcgcgagat cgtccggcgt ccaggttttc cacggcagat
    18961 tatcagacat aggtatcctc gccgctgcca attaccatct cgccagtttc ggcaaggcgg
    19021 cgcacaatca gcagaatcgc tttctgttcg ttttccacct gcgacagacg caccggacca
    19081 cggttggcga gatcgtcgcg cagaatatcg gcggcacgct gcgacatatt gcgcaggaat
    19141 ttctcgcgca gtggctgctc ggcccctttc agcgcgatca acagcgattc ggaatccact
    19201 tcctgcaaca gacgctgaat gctgcgatcg tcgacatcca ccagattttc gaacaggaac
    19261 atctcgtcga taattttttg cgccagctcg ccgtcgaatt cgcgcacggc ggtaataacg
    19321 gcttcttcct gctgagtttt catcaggttg ataatttcgg ctgccgttct cacgccgccc
    19381 attttgctgc gcttgagatt ctgaccgtcg agcaagccat tcagtacttc ggtcagttcc
    19441 gccagcgcgg ctggctgcac gccgccaaag gtggcgatac gcaacatcac gtcgtggcgc
    19501 agacgttcat cgaacaacgc cagaatatcg gcggcctgcg cgcgcttcag atgcaccaga
    19561 atggtggcga taatttgcgg atgctcatcg cgaatcagat cggcggcact ctgcggctcc
    19621 ataaagttga gcgtttcaat accgctggcg gtatcgcgag tttcgagaat atcttccagc
    19681 aggctggcgg cacgttcctc acccagagct ttgaccaata ccgaacgcag ataatcgttg
    19741 gcgttgatat tcagtgcggc aaactgttca gcttcttgct caaactccgc cagcacatag
    19801 gttagctgct tgttggagat ctgcgtgacg ttcgccattg cagcgctcag ggtttgcact
    19861 tcgcgctggg agaggtgctt gaacacctct gccgcccggt cttcgccaat ggtcatcagc
    19921 aggatgacgc ttttatcggt gcctgtcagg ttactcatga tcgttattta tccactggcg
    19981 aatgaccagc gccaccacgc gcggatcgtt atcagacatt tcacggatac gctggctcat
    20041 gacttctgcc cccagacgtt ggttagcgcg tcgttgttgg agttgttcgt ctttgctcag
    20101 gcggacttcc accgcatctt ccacttcctc acgggcctgc gcctgttgct gtacagcttt
    20161 catcgcctcg gcgcgacgtg ttaactgcgg gcgtaccgct ttccgccaca gcagccacgc
    20221 caccagcagt accagcaacc agcgaccggc agccagcaac tgatcgataa acgcttgctg
    20281 ttgccagaac ggcagttctc cgccgctttc gtcactgcta ttgaacggcg agttaacgac
    20341 attgagcgag tcaccgcgtt tttcagaaaa gcccatcgct tcgcgggtca gatcttcaat
    20401 ttgcttcatc tgttcgttgc tgagaggcaa tggtttaccg tctggcaagg ttttgtaatt
    20461 caccactacc gcgactgaca gacgttgcac atcgcccacg ttcattttgg tatgacgaat
    20521 ggtgcgatcg acttcgtagt tactggtttc attccgctgt gtgctacgcg gcccgctatt
    20581 gctggtggtg ctcgcctgct gctggcggtt attttgattt gccggaggcg tgctgattgg
    20641 cgcgttattc gcaggtgccg gttgattcga caacgccccc ggtacgccgc ccggataacc
    20701 ggaaccgctt tgctcgctct cattaagctg gcgtgaacga agcgccgcat gagattcatc
    20761 accgttaggg cgatactgtt cttccgtttg ttctttactg gcgaagtcca gttgcgccgt
    20821 gacctgggcg tgaatattac cgttaccaac aataggcgac aggattgctt caatacgccg
    20881 ctgaatacgg ccttcgacat cgctggcata tttcaactga gcgtcattaa gatcgcgccc
    20941 gctggtattg gactgggtta acagatgtcc gccctgatcc accagcgtga cgtttcccgg
    21001 cggcagacca gcaacggcgc tggaaaccag gtgcacaatg gcgctaattt gcccttcatc
    21061 gagtgcgcgg ccgggtaaca gatttaccgt caccgatgca gaaggggatt tttgttcacg
    21121 gacgaataaa gacggtttcg gcattgccag atgtacgcgc gcccctttta ccgggccgat
    21181 agtttcgatg gtacgagaaa gctcgccttc cagcgcccgc tgataattca cctgttcgct
    21241 gaactggctg ataccaaact tttcctgatc aagcagttcg aaaccgaccg cgccgccttt
    21301 gggcaaacct tgttgtgcca ggcgcagacg cagttcgtga actttatctg ccggaacttc
    21361 aatagcgccg ctggcttcgc tgaagcagta aggaatattc atttgcgtca gttggctgac
    21421 aattgcgcca ccatcctgat cggaaagatt gctgaataat gtgcggtagt cgggggcttt
    21481 cgcccacagg atcagtgcga ccatgaccgc cactgccgcg gaaccggcaa caatcaatgg
    21541 aattttcgga ttcgcacgca ggcgattaag ccactcaaga gattttgtct gggctgcagt
    21601 cgcattcatc gcgcacctcg tggctgatta tggacggcgt tattggcaaa gtggaacaag
    21661 actcactccc gggtcagcaa actcgaaaaa ttgacgctgc cattatttgt gctttcgttt
    21721 ttttctatcc gccaataaac ccgttttttt tgttgctatt tagcgccttt gtcttattga
    21781 cttactggta agctttgcta ccagaaatta tccgggagat gaaaatgtca gcgatacagg
    21841 ggattgaagg ggttatcagc cagttacagg ctacggcgat gagtgcgcgt gcgcaggaat
    21901 cactgccaca accgaccatt agttttgccg ggcagctgca cgccgcgctc gatcgcataa
    21961 gcgatacaca aacagccgcc cgcacgcagg cagaaaaatt cactctcggt gaacccggcg
    22021 tggcgttaaa cgatgtgatg accgatatgc aaaaagcctc agtttctatg caaatgggga
    22081 ttcaggtgcg taataagctg gtggcggctt atcaggaagt gatgtccatg caggtgtagg
    22141 ttttgtaacc tgttgttaat tacatccgat ctcaccgcgt ggggcatgga tggggcaaac
    22201 tcactcaatt tctggtttaa aatggatacc tgatcctgaa agttttctgc catccacgat
    22261 ccgtaaacgc gataacatgt tttgtcttcg ctttgctcat aggaagagtg aactcttttg
    22321 ttagcctggg gttccttctg atcattattg ttcctgcctt tatctcttgc cacgccagag
    22381 ccccctggat acacatacac caggatgaaa acggtgagtt gtgttcaaac tgtagtacaa
    22441 ttctctccag tatgaacagg aaagaatatg ctatgaaccc ttatatttat cttggtggcg
    22501 caatacttgc agaggtcatt ggtacaacct taatgaagtt ttcagagggg tttacacggt
    22561 tatggccctc tgttggcaca attatttgct attgtgcatc attttggtta ttagctcaga
    22621 cactggctta tatacctaca gggattgctt atgctatctg gtcaggagtc ggtatcgttc
    22681 tgattagttt gctttcctgg ggattttttg gccaacgact ggacctgcca gccatcatag
    22741 gcatgatgtt gatttgtgcc ggtgtgttag tcattaactt gttgtcacga agcgcaccgc
    22801 attaaaaaat aatcatcctt gagataacag gaatctgatt tgtgcatatt atggctaatg
    22861 ttcggggcaa ctttataatt atgattgcat ttgcgcgatc attgcgcctt tttgcataat
    22921 gagatcaatg cacatcaaat aaaataacga taggtcacaa caaagaggtt tttatgaaaa
    22981 aacttatcgt ttcaagtgta ttggcattca taacattttc tgcgcaggcc gcagcatttc
    23041 aggtcactag taatgaaata aaaacaggag agcaacttac aacgtctcat gtattttctg
    23101 gagttgggtg tgaggggggt aatacatcgc cctcattaac ctggtctggt gctccagaag
    23161 gcaccaaaag ctttgccgta actgtgtatg atccagatgc gcctacaggc agcggatggt
    23221 ggcactggac tgttgctaat attccagcaa cggtaacata tttgcccgct gatgcaggaa
    23281 gacgtgatgg tacaaaactt ccgactggtg ctgtccaagg ccgaaatgat tttggctatg
    23341 cgggatttgg tggagcatgt cctcccaaag gagataaacc ccatcattac cagtttaaaa
    23401 tatgggctct aaaaactgat aagattcctg tggattctaa ttccagcgga gcgttagttg
    23461 gttatatgct taatgccaat aaaattgcaa ccgctgagat aatacccgtt tatgaggtaa
    23521 agtaggataa gagtatgctg gcaaaagata ggactaacct aaagattgaa gaaatacgga
    23581 tgcataaaca tcatgagatt cacagggtta agcctcttat gccagcttca tgtcgtatcc
    23641 gtcagggaaa aaaagttatc aattgggaga cgcatagtct aactgttgat aataatcaga
    23701 taatattatt tccctgtgga tatgaatttt atattgcgaa ttacccagaa gcaggtcttt
    23761 atcttgcaga aatgctttat tatcccattg atctaattga aaagttccaa aatctttatg
    23821 cgataaccga tcaaattcgt aacacgacag gtttctgctt acctcagaat tccgagttaa
    23881 tatattgttg ggagcaacta aaaacatcta tttcccgagg cttctcaacg caaattcagg
    23941 aacacttagc aatgggcgtt ctactttcat taggagcaca tcatgttaat tgtttacttt
    24001 tatcagatag taaacaatca ttaataagtc gttgttataa cctaatgcta tccgaacctg
    24061 gaacaaaatg gacagcaaac aaggtagcga gatatctcta catttctgtt tccacattgc
    24121 atcgccgtct ggcaagcgag ggagtaagtt ttcaaagtat attggacgac gtgaggttaa
    24181 ataatgcgtt gtctgctata caaacgacag taaaacccat cagcgagatt gccagggaaa
    24241 atggttacaa gtgtccttct cgttttactg aaaggttcca taatcgtttt aagataacac
    24301 caagagagct aagaaaagcg tccagagagt aaaagtattt tatgaaggag caacagcatc
    24361 gatttttatt ttggaaaata aatacggcat actcaaccta ttggagcctg aacaatccgg
    24421 tgacttctgc gctaatcggg gacgtttata agcacatata attgatacta tcttcctcca
    24481 tcacaatttc agaaatgcac gtgtgatttt tttgcatccg gcgtttggac tatgtggagt
    24541 tcaagagtgg gaaagttaca agaggttgag gggataatta aagggatgct tatgacatta
    24601 gatatccgga taggtacgga tatcgttgtt attaaatcta gttcgttatt attaaatcta
    24661 gtttatacgt taagatgcag ctacagcaga aattgccact gttaattttt tcatcgtgag
    24721 cccttttatt tgaactatta tttaaaaatg atgtcactgc actacaaata ttcatccaat
    24781 caatgagatt atttcaagat gtaagtttta gtttctcatt taaattgtga agtagat
//
LOCUS       DAETQN010000060        24447 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.060, whole genome shotgun sequence.
ACCESSION   DAETQN010000060 DAETQN010000000
VERSION     DAETQN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.060"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..395
                     /locus_tag="K0W68_002384"
     CDS             <1..395
                     /locus_tag="K0W68_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI3073922.1"
                     /translation="YIIHRKNLLITGPTGCGKSWIANALGEQACRQKYSVRYCRTGRL
                     LEQLAQGRVDGSWLKYLKQLQKIQVLISSTIVVSQFPVDKWHGLMENPTTADAILDRL
                     VHNSHRVVLQGESLRKNPPTVESSEKTS"
     gene            complement(534..896)
                     /locus_tag="K0W68_002385"
     CDS             complement(534..896)
                     /locus_tag="K0W68_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBI3073923.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            1433..3346
                     /gene="mtlA"
                     /locus_tag="K0W68_002386"
     CDS             1433..3346
                     /gene="mtlA"
                     /locus_tag="K0W68_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBI3073924.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3576..4724
                     /gene="mtlD"
                     /locus_tag="K0W68_002387"
     CDS             3576..4724
                     /gene="mtlD"
                     /locus_tag="K0W68_002387"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBI3073925.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4724..5311
                     /gene="mtlR"
                     /locus_tag="K0W68_002388"
     CDS             4724..5311
                     /gene="mtlR"
                     /locus_tag="K0W68_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBI3073926.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(5323..5532)
                     /locus_tag="K0W68_002389"
     CDS             complement(5323..5532)
                     /locus_tag="K0W68_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3073927.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5817..6179
                     /locus_tag="K0W68_002390"
     CDS             5817..6179
                     /locus_tag="K0W68_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBI3073928.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            6722..7400
                     /locus_tag="K0W68_002391"
                     /pseudo
     CDS             6722..7400
                     /locus_tag="K0W68_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            7444..12294
                     /locus_tag="K0W68_002392"
     CDS             7444..12294
                     /locus_tag="K0W68_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HBI3073929.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            12662..14317
                     /gene="lldP"
                     /locus_tag="K0W68_002393"
     CDS             12662..14317
                     /gene="lldP"
                     /locus_tag="K0W68_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBI3073930.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            14317..15093
                     /gene="lldR"
                     /locus_tag="K0W68_002394"
     CDS             14317..15093
                     /gene="lldR"
                     /locus_tag="K0W68_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBI3073931.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            15090..16280
                     /gene="lldD"
                     /locus_tag="K0W68_002395"
     CDS             15090..16280
                     /gene="lldD"
                     /locus_tag="K0W68_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBI3073932.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            16476..16949
                     /gene="trmL"
                     /locus_tag="K0W68_002396"
     CDS             16476..16949
                     /gene="trmL"
                     /locus_tag="K0W68_002396"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBI3073933.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(17002..17823)
                     /gene="cysE"
                     /locus_tag="K0W68_002397"
     CDS             complement(17002..17823)
                     /gene="cysE"
                     /locus_tag="K0W68_002397"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBI3073934.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(17903..18922)
                     /gene="gpsA"
                     /locus_tag="K0W68_002398"
     CDS             complement(17903..18922)
                     /gene="gpsA"
                     /locus_tag="K0W68_002398"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBI3073935.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(18922..19389)
                     /gene="secB"
                     /locus_tag="K0W68_002399"
     CDS             complement(18922..19389)
                     /gene="secB"
                     /locus_tag="K0W68_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBI3073936.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(19452..19703)
                     /gene="grxC"
                     /locus_tag="K0W68_002400"
     CDS             complement(19452..19703)
                     /gene="grxC"
                     /locus_tag="K0W68_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBI3073937.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(19845..20276)
                     /locus_tag="K0W68_002401"
     CDS             complement(19845..20276)
                     /locus_tag="K0W68_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBI3073938.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            20521..22065
                     /gene="gpmM"
                     /locus_tag="K0W68_002402"
                     /gene_synonym="pgmI"
     CDS             20521..22065
                     /gene="gpmM"
                     /locus_tag="K0W68_002402"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBI3073939.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            22075..23358
                     /gene="envC"
                     /locus_tag="K0W68_002403"
     CDS             22075..23358
                     /gene="envC"
                     /locus_tag="K0W68_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBI3073940.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            23362..24321
                     /locus_tag="K0W68_002404"
     CDS             23362..24321
                     /locus_tag="K0W68_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBI3073941.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
BASE COUNT         6207 a         6214 c         6347 g         5679 t
ORIGIN      
        1 actacataat ccacaggaaa aacctactga taaccggtcc gacaggatgc ggtaagagct
       61 ggatagccaa tgcgctgggc gaacaggcat gtcgacagaa atacagcgtc cggtactgta
      121 gaaccgggcg attactggaa cagttggctc agggacgtgt tgatggcagt tggctgaaat
      181 atctgaaaca gttgcaaaaa atacaggttc taataagcag cacgatagtg gtaagccagt
      241 tcccggtaga taaatggcat ggtctgatgg agaacccaac aacagcagat gccatcctgg
      301 acagactggt acataactca cacagagtgg tccttcaggg agagtccctg aggaaaaatc
      361 ccccgacagt ggaaagcagc gaaaaaacga gttaaaaagc ggtagaaaaa gttaagttcc
      421 cggttgatcg aatggatgcg aatcgctgat cgaacttcga tgccaatccg tgatcgaata
      481 gagcggaata cgcaccagca ccacgtaagt taaaacaatc aatagatcca taattagatc
      541 tccgggaacg tggaatagtc agtctttttc tcggcggcga gttgatgctc tagccgggaa
      601 attcggtcag aaagtgcggc gatttccggg tcagtcgctt tctcctgcga cgcaacatgt
      661 gactgcatgc cccacccgcg ctccggttga tagagcgtcg cccagatcca caggaacggc
      721 cagataacat gcagggtaaa caggctcacc cagcccgccg tatgaatagc gtcggcatgg
      781 ggatggttgc gctttttggc aatcagataa gggatgtcat gtatcgcgat gattccataa
      841 aaaatcacca gaaagacgaa gatcagcact cccaacgcga aatagtttag aaacatatct
      901 gcctcggatt cacgtttatc agtgttgttt ttgggctggc agccagaagg gagtcaggct
      961 gatattttga caataatccg ggttcgcgat tctcgccata acaccaaaga ataattttta
     1021 gaggtgatga gttgcttagt tacataacaa ttgtatgacg aaggcataac atgctgtaga
     1081 tcacatcagg tgaacgccgt aagaaaatat cttgtgattc agatcacaaa gattcaacaa
     1141 accatcaaaa caaaaatgtg acactactca catttaaatg ccatttttag cgaaaatcgc
     1201 cgccttgttg cttttttaca caagcgtttt gtgatgaacg tcacgttaat tacctctcta
     1261 ccccctatat ttatgtgatt gatatcacac aaaaggccgt cgactggaca gttaaccgat
     1321 tcagtgccag atttcgcagt atctacaagg tccggctacc tctgccgcca cattaacaaa
     1381 aaacctcggg cttccagcct gcgcgacagc aaacataaga aggggtgttt ttatgtcatc
     1441 cgatattaag atcaaagtgc aaagctttgg tcgtttcctc agcaacatgg tgatgccaaa
     1501 tatcggcgcg tttatcgcgt ggggtatcat caccgcgtta tttattccaa cagggtggtt
     1561 accgaacgag acgctggcga agctggtcgg gccgatgatc acttatctcc tgccgctgct
     1621 gatcggttat accggtggta agctggtagg cggcgaacgt ggcggcgtag tcggtgccat
     1681 caccaccatg ggcgttatcg tcggcgcaga catgccgatg ttcctcggtt ctatgattgc
     1741 aggtccgctg ggcggctggt gcattaagca cttcgaccgc tgggtagacg gtaagatcaa
     1801 atccggtttt gagatgctgg tgaataactt ctccgcaggc atcatcggga tgatcctcgc
     1861 tattctggca ttcctcggca ttggcccgat tgttgaagcc ctgtccaaaa tgctggctgc
     1921 gggcgttaac ttcatggttg tccatgacat gctgccgctg gcgtctatct ttgttgaacc
     1981 ggcgaaaatc ctgttcctca acaacgccat taaccacggt atcttctcgc cgctgggtat
     2041 tcagcagtcc catgaactgg gtaaatcaat cttcttcctg attgaagcta acccaggtcc
     2101 aggtatgggc gtgctgctgg cgtacatgtt ctttggtcgt ggtagcgcta aacagtctgc
     2161 gggcggtgcg gcaatcatcc acttcctggg tggtatccac gaaatctact tcccgtatgt
     2221 gctgatgaat ccgcgtctga tcctcgcagt catcctcggc ggtatgactg gcgtgttcac
     2281 gctgactatc ctgggcggtg gtctggtttc tccggcatct ccgggttcta tccttgctgt
     2341 actggcgatg acaccaaaag gtgcttattt cgctaacatc gcgggtgtgt gtgcggcgat
     2401 ggctgtctcc ttcgttgtct ctgctatttt gctgaaaacc agcaaagtga aagaagaaga
     2461 tgatattgaa gcagcaactc gtcgtatgca ggacatgaaa gctgagtcta aaggcacatc
     2521 tccgctgtct gcgggcgatg tgactaacga cctgagccac gtacgtaaaa tcatcgttgc
     2581 ctgtgacgcc ggtatgggtt ccagtgcgat gggcgcaggc gtgctgcgta agaaaattca
     2641 ggatgcaggt ctgtcacaga tttcggtcac taacagcgcg atcaacaacc tgccgccaga
     2701 tgtggacctc gtcatcactc accgtgacct gaccgaacgc gctatgcgcc aggttccgca
     2761 ggcgcagcat atttcgctga ccaacttcct cgacagcggc ctgtacacca gcctaaccga
     2821 acgtctggtt gctgcccagc gccatactga aaacgaagtg aaagtgaaag acagtctgaa
     2881 agacagcttt gacgattcca gtgctaacct gttcaaactg ggcgcggaga acatcttcct
     2941 cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt gctggcgagc agctggtgaa
     3001 aggcggttac gttgagccgg aatacgttca ggcgatgctg gatcgtgaaa aactgacccc
     3061 gacttatctg ggtgagtcta tcgcggtgcc acacggtacg gttgaagcga aagatcgcgt
     3121 actgaaaacg ggcgtggtgt tctgccagta cccggaaggc gtgcgcttcg gtgaagaaga
     3181 agatgacatt gcccgtctgg tgattggtat tgcagcccgt aacaacgagc acattcaggt
     3241 tatcaccagc ctgaccaatg cactggatga tgagtccgtc atcgagcgtc tggcacacac
     3301 caccagcgtg gatgaagtgc tggaactgtt ggcaggtcgt aagtaatcca atcccaccct
     3361 ctccacatgg agaaggtggg gttaattgcc tgatgcgcta cgcttatcag gcctacagga
     3421 tgcatcacga tttgttgaat ttgcacgttc ttgtaggccg gataaggcgc ttacgccgca
     3481 tccggcgctg cccctctcct cacggagagg gtttgggtga gggaaaagcc tcaccccagc
     3541 cctctcgggt aaaaacattg atgaaggtta atactatgaa agcattacat tttggcgcag
     3601 gtaatatcgg tcgtggcttt atcggtaaac tgctggcaga cgcgggtatc caactgacgt
     3661 ttgccgatgt caatcaggtg gtacttgatg ccctgaatgc ccgtcatagc tatcaggttc
     3721 atgtggttgg tgaaaccgag caggtagata ccgtttccgg cgtcaatgct gtcagcagca
     3781 ttggtgatga tgtcgttgat ctgattgctc aggtcgattt agtcactacc gccgttggcc
     3841 cggttgtgct ggaacgtatt gctccggcta tcgccaaagg gctggtgaaa cgcaaagaac
     3901 aaggtaatga atccccgctg aacatcatcg cctgtgaaaa catggtacgc ggtaccacgc
     3961 agctgaaagg ccatgtgatg aacgccctgc cggaagacgc caaagcgtgg gtagaagaac
     4021 acgttggctt tgtcgattcc gccgttgacc gcatcgtacc gccttcggct tcggcaacta
     4081 acgatccgct ggaagtgacg gtagaaactt tcagcgaatg gattgtcgat aaaacgcagt
     4141 tcaaaggcgc actgccgaac atcccaggca tggagttaac cgacaacctg atggcatttg
     4201 tcgaacgtaa actcttcacc ctgaacacgg gtcatgctat aaccgcgtac ctcggaaaac
     4261 tggccggtca tcagaccatt cgtgacgcta ttctcgacga gaaaatccgc gcggtggtaa
     4321 aaggtgcgat ggaagaaagt ggtgcggtac tgatcaagcg ctacggcttt gacgcagaca
     4381 aacatgcggc gtacatccag aaaatcctcg gtcgttttga gaacccgtat ctgaaagatg
     4441 atgtagagcg cgtaggccgt cagccgctgc gtaaactgag tgctggcgac cgtctgatca
     4501 agccattgct cggtacgctg gaatacagcc ttccgcacaa gaatctgatt caggggattg
     4561 ctggtgcaat gcacttccgc agtgaagatg atccgcaggc tcaggaactg gcagcactga
     4621 tcgctgacaa aggtccgcag gcggcgctgg cacagatttc cgatcttgat gccaacagcg
     4681 aggttgtatc cgaggcggta accgcttata aagcaatgca ataatggtgg accaggcgca
     4741 ggacaccctg cgcccgaata acagattgtc agatatgcag gcaacaatgg aacaaaccca
     4801 ggcctttgaa aaccgtgtgc ttgagcgtct gaatgctggc aaaaccgtgc gaagctttct
     4861 gatcaccgcc gtcgagcttt tgaccgaggc ggtaaatctt ctggtgcttc aggtattccg
     4921 caaagacgat tacgcggtga agtatgctgt agaaccgtta ctcgacggcg acggtccgct
     4981 gggcgatctt tctgtgcgtt taaaactcat ttacgggttg ggcgtcatta accgccagga
     5041 atacgaagat gcggagctgc tgatggcatt gcgtgaagaa ctaaatcacg acggcaacga
     5101 gtacgccttt accgacgacg agatccttgg accctttggt gaactgcact gcgtggcggc
     5161 gttaccaccg ccgccacagt ttgaaccagc agactccagt ttgtatgcaa tgcaaattca
     5221 gcgctatcaa caggctgtgc gatcaacaat ggtcctttca ctgactgagc tgatttccag
     5281 aatcagctta aaaaaagcct ttcaaaagta agcaacgtct gcttactgcc cctctacctg
     5341 cttcggccga taaagccgac gataatactc cagacgttgt agatataacg gcacgctttc
     5401 atcgggtatt ccggacggaa tcgcgttacg gggaggaagt tttttcagat actcccggaa
     5461 cgcctggctt gatgccatga aatctacggc tttatcgata agaagcggaa cgttttcacc
     5521 taatttgccc atgatgatat ctccgtatta ccccgcgccg ggaatgcgcg gccgccaatt
     5581 ttagtttagg ttcccggtaa agatgacatt aaggaaaacg tgctgaatcc tcaataatca
     5641 tccgctgctg tttattatct tttttctata gatttcttaa ttaatcaata aattgcgtag
     5701 tttttaagca tgttaatgat tacgttacag aactattgca atgtcatcag ttgtgaacaa
     5761 agcacctggt cgcgcatact aggggctata aatttatctt tatcagaagc catcacatga
     5821 aagaagtcga aaaaaacgaa atcaaacgtc tcagcgatcg tctggacgcc atccgccacc
     5881 agcaggccga tctgtcgctg gttgaagccg cagacaaata tgccgagctg gaaaaagaga
     5941 aagccacgct ggaagcagaa attgctcgcc tgcgtgaagt tcatagccaa aaactgagta
     6001 aagaagcaca gaagctgatg aagatgccgt ttcagcgcgc aattaccaaa aaagagcagg
     6061 ctgatatggg caagctgaag aaaagtgttc gcggactggt ggttgtgcac ccaatgaccg
     6121 cgctgggccg tgaaatgggc ctgcaggaga tgactggatt ctctaagact gcgttttaag
     6181 cgcctcagac gactggttcg atcatatcaa cccgcatctg gcttgcctta gctggtcaat
     6241 gcggggactc tcctgtctac tcgcctaatt accaaacaat tcactccctt tacgtcgatt
     6301 taccgctggt tgagattctg aaaatgcatt tttaccgaaa tagccaccta taaacaataa
     6361 aaatatatag aagatatcat ttattaatta acaagcagtt acgtctcttt ttttcggatt
     6421 taattgtgaa attttaatca tcatattcat acaataaatt atctgtaaat tttttcccag
     6481 acatggtttc tttcatattt gacatttatt aataatcatc attagaatcc acgaaaataa
     6541 tatattcata caaacacaga catcttttga aacgaatgat acatttttat gatgaatgct
     6601 taaaacaata tttaatcgac acacatcact gtcattaatg taaagcaaca tagatcacca
     6661 ataatacaat ggacaactct accggcattg atcgctggtc ggaggaaata aggattttta
     6721 catgaacttg aagaagattt ttttcagtgc tgtcactgtt tcagttctct gcgcattaac
     6781 gggatgtgac tacattgaag aaggcaagcc agaaagtagt ttattgaaac aacaagaaga
     6841 acattaaaat tgatctactt gaaaaacaac aagcacagct aaagagtcaa ctggaaacta
     6901 ttcaaaaaca acaaactggc ataataagca gtacaaagac tttaactcac gtaattaaat
     6961 ccgttaaaga tcaacaaaac acttttattt ttaccgagtt taacccagca aaaacgaagt
     7021 atttcatcct gaacaatggt tcggtggctt tagcgggtcg agtgttatcc attgacgcca
     7081 cagagaatgg cagtgttatt catatttcac tggtcaactt attaagtaca cctatctcaa
     7141 atattggttt taatgcgaca tggggtggtg aaaaacctgt cgatgccaaa gagtttgccc
     7201 ggtggcaaca attacttttc aacacatcaa tgaaatccac attgaaatta ttaccaggtc
     7261 aatggcaaga cattaatttg accctgaagg gtgtatcgcc caataacctg ggatatctga
     7321 agttagccat caacatggaa aatattcagt tcgacaatct tccctctgct gaaaatcggc
     7381 agaaaagaag caaaaaataa atgcagttag tacgcttata acaaggaata gcgagcatca
     7441 ttaatgaaca aaatatttaa agttatctgg aaccctgcga cagggaatta tactgttacc
     7501 agcgaaacgg caaaaagccg tggcaagaaa tctgggcgca gtaagctgtt aatttctgcg
     7561 ctggttgcgg gtggaatgtt gtcgtcgttt ggggcattgg cgaatgccgg gaatgacaac
     7621 ggccagggtg ttgattacgg tagtggatca gctggcgaca gctgggttgc tataggcaaa
     7681 ggggcgaaag caaatacttt tatgaacacc agtggttcca gtactgctgt gggttatgac
     7741 gctatagctg aaggccaata tagctctgcc atcgggtcaa aaacccatgc gattggtggt
     7801 gcatcaatgg cctttggggt tagtgcaata tcagaaggcg atagaagtat agcactgggt
     7861 gcctcttcgt attcattggg ccaatactca atggccctcg gccgttattc aaaagcattg
     7921 ggtaaattgt ctattgctat gggggactct tccaaagcgg aaggagcaaa cgccattgcc
     7981 ctgggaaatg ccactaaagc tactgagatt atgagtattg ctcttggcga caccgccaat
     8041 gcgtcaaaag cgtattcaat ggcgctggga gcaagtagcg tcgcatctga agaaaacgca
     8101 attgccctgg ggcgtagcag tgtagctagc ggtactgaca gcctcgcatt tggcagacaa
     8161 tcacttgcca gcgcagcgaa cgctattgcg ataggtgctg agaccgaagc cgctgaaaat
     8221 gcaactgcta ttggcaataa tgcgaaggca aaagggacta atagcatggc aatggggttc
     8281 ggaagccttg ccgataaagt caatactatc gcattaggaa atggcagcca ggctctggca
     8341 gataatgcaa tcgccatagg ccagggcaac aaagctgatg gcgtggatgc catcgctctg
     8401 ggtaatggta gccagtcgag aggcttaaac accattgcct taggcacagc cagtaatgca
     8461 actggtgata agagtcttgc gcttggtagt aatagcagtg ccaacggtat taactctgtc
     8521 gcgctgggcg cagattccat tgcggattta gacaataccg tctctgtcgg caatagttca
     8581 ttaaaacgca agatcgttaa tgtgaaaaat ggcgcgatca agtctgacag ttacgatgcc
     8641 attaatggtt cacagcttta tgccattagc gactcggtag caaaaaggct tggaggaggg
     8701 gctgcagtag atgttgatga cggtactgtt acagcaccaa cctacaattt aaaaaatggt
     8761 agcaaaaata acgtaggggc tgcgctcgct gtacttgatg aaaacaccct gcaatgggac
     8821 caaaccaaag gcaaatacag cgctgctcat ggtactagta gcccaactgc cagcgtaatc
     8881 accgatgttg cggatggcac gatttcagcc tccagtaagg atgcggttaa cggttcccaa
     8941 ctgaaagcta ccaatgacga tgtcgaagcc aacaccgcca atatcgctac taataccagc
     9001 aacattgcca cgaatacagc aagtattgcc accaatacca ccaatatcac caacctgacg
     9061 gattccgttg gtgaccttca ggctgatgcc ctgctctgga acgaaactaa aaaggcattc
     9121 agtgcagctc acggccagga taccaccagc aaaatcacca acgttaaaga tgccgacctg
     9181 acggctgaca gcactgatgc tgttaacggc tctcagctga aaaccaccaa cgatgctgtg
     9241 gcgacgaata ccaccaatat cgccaataac acttccaata ttgccactaa caccaccaac
     9301 atctctaatc tgactgagac ggtgactaat cttggtgagg atgcgctgaa atgggataag
     9361 gacaatggtg tattcacggc agctcatggc accgagacca ccagcaaaat caccaacgtt
     9421 aaagatggcg acctgacgac tggcagcacc gatgccgtta acggctctca gctgaaaacc
     9481 accaacgatg ccgtggcgac gaataccacc aatatcgcca ctaacaccac caacatctct
     9541 aatctgactg agacggtgac taatcttggt gaggatgcgc tgaaatggga taaggacaat
     9601 ggtgtcttca ctgcagctca tggcaacaat accgccagca aaatcaccaa tatcctggac
     9661 ggcacagtca ctgcaaccag ttccgatgcc attaacggta gccagcttta tgacttaagc
     9721 agcaatatcg ccacctactt cggcggcaat gcttctgtga atactgacgg tgtgtttacc
     9781 ggtccaacct acaaaatcgg tgaaacaaat tattataacg tcggcgatgc actggctgcg
     9841 attaactcct catttagcac gtctctcggc gatgctctgc tttgggatgc caccgcaggt
     9901 aaattcagtg ccaaacacgg tactaatggt gacgcaagcg tgatcactga tgtcgcagat
     9961 ggtgaaattt cagactccag ttctgacgca gtaaacggct cacaactcca cggcgtgagc
    10021 agttatgttg ttgatgcgct ggggggtggt gccgaagtca atgcagacgg caccatcact
    10081 gcgccgacgt acaccattgc taatgctgat tacgataatg tcggtgatgc cctgaatgct
    10141 atcgatacca ctcttgacga cgctctgctc tgggatgcgg acgccggtga aaatggtgca
    10201 tttagcgccg ctcacggaaa agataaaact gccagtgtaa tcactaacgt cgctaacggt
    10261 gcaatctctg ctgccagcag cgacgcgatt aacggctcac aactctatac caccaataag
    10321 tacatcgctg atgcgctggg tggtgacgca gaagtcaacg ctgacggcac catcaccgca
    10381 ccgacttaca ccattgcgaa cgccgagtac aacaacgtcg gtgacgccct ggatgcgctt
    10441 gatgataacg ccctgctgtg ggatgagact gccaatggcg gtgctggagc ctacaatgcc
    10501 agccatgacg gtaaagccag catcatcact aatgtcgcta atggcagtat tagtgaggac
    10561 agtaccgatg cagtgaacgg ttctcagttg aatgcgacga atatgatgat tgagcagaac
    10621 acccaaatta tcaatcagct cgctggtaac accgacgcaa cctatatcga agaaaatggc
    10681 gctggtatca actacgttcg tactaacgac aacgatttag cctttaacga tgcaagcgcc
    10741 tctggtgtcg gcgctacagc tgtaggttat aacgctgtcg cgtctggtgc cagcagcgta
    10801 gccattggtc agaacagcag cagcaccgtt gataccggta ttgcgctggg tagcagctcc
    10861 gtttccagcc gtgtgattgc caaaggttct cgtgacacta gcgtaacgga aaatggcgtg
    10921 gtgattggtt atgacaccac tgacggcgaa ctgctaggtg cattgtcaat tggtgatgac
    10981 ggtaaatacc gccaaatcat caacgtagct gatggttcag aagcccatga cgccgttacg
    11041 gttcgccagt tgcagaacgc tattggagcg gtcgccacta cgccaaccaa gtactttcac
    11101 gccaactcaa cggcagaaga ctcactggcc gttggtgaag actcgctggc aatgggtgcg
    11161 aaaactgtcg ttaatggtaa tgcaggtatt ggtatcggtt tgaacactct ggttctagct
    11221 gatgcgatca acggcattgc tatcggcagt aacgcacgcg caaaccatgc aaacagtatc
    11281 gcaatgggta acggttctca gaccacccgt ggtgcacaga ctggctacac cgcctacaac
    11341 atggacgcac cgcagaactc tgtaggtgag ttttctgtcg gtagtgaaga cggtcaacgt
    11401 cagatcacaa acgtcgcagc tggttcagcg gataccgatg cggttaacgt gggtcagttg
    11461 aaagtcactg atgagcgcgt agcgcaaaat acccagagca ttactaacct gaacaatcag
    11521 gtcactaatc tggatactcg cgttactaat atcgaaaacg gtattggcga cattgtcacc
    11581 accggtagca ccaagtactt caagaccaac accgatggcg tagatgccaa cgcccagggt
    11641 aaagatagcg ttgctattgg ttctggttcc attgctgccg ctgacaacag cgtcgcactg
    11701 ggtaccggtt ccgttgcaga ggaagaaaat acaatctctg taggttcttc tactaaccaa
    11761 cgccgtatca ccaacgtagc tgccggtgtt aatgccaccg atgcggttaa cgtgtcgcag
    11821 ctgaaatctt ctgaagcagg cggcgtgcgc tacgacacca aagctgatgg ttctatcgac
    11881 tatagcaata tcaccctcgg tggtggcaac ggcagtacga ctcgtatcag caacgtctcc
    11941 gctggcgtca acaacaacga cgcggtgaac tacgcgcagt tgaagcaaag cgtgcaggaa
    12001 acgaagcaat acaccgatca gcggatggtt gagatggata acaaactgtc taaaactgaa
    12061 agcaagttga gcggtggtat cgcttctgca atggcaatga ccggtctgcc gcaggcttat
    12121 acaccgggtg ccagcatggc ttctattggt ggcggtactt acaacggtga atcggcagtt
    12181 gctttaggtg tatcgatggt gagcgccaat ggtcgttggg tctacaaatt acaaggtagt
    12241 accaatagcc agggtgaata ctccgccgca ctcggtgccg gtattcagtg gtaatgatcc
    12301 attaagttag tgtgactaag ggcaaacgcc aaattgcctg atgcgttacg cgcatcaggc
    12361 aagataaggc gccccgggtc aacaatctaa gggcaattct ctgatgagga ttgccctttt
    12421 ctttaccaga catctccccc cacaagaatt ggccctacca attcttcgct tatctgacct
    12481 ctggttcaca attccccaat taaaactcac atcaatattg ccaatacata acatttagtt
    12541 aaccattcat tgtcattatc cctacacaac acaattggca gtgccacttt tacacaacgt
    12601 gtgacaagga gatgagcaac agactcatta cacgatgtgc gtggactcca ggagacctgc
    12661 aatgaatctc tggcaacaaa actacgatcc cgccgggaat atctggcttt ccagtctgat
    12721 agcatcgctt cccatcctgt ttttcttctt tgcgctgatt aagctcaaac tgaaaggata
    12781 cgtcgccgcc tcatggacgg tagcaatcgc ccttgccgtg gctttgctgt tctataaaat
    12841 gccggtcgct aacgcgctgg cctcggtggt ttatggcttc ttctatggtt tgtggcccat
    12901 cgcgtggatc attattgcag cagtgttcgt ctataagatc tcggtgaaaa ccgggcagtt
    12961 tgacatcatt cgctcgtcta ttctttcgat aacccctgac cagcgtctgc aaatgctgat
    13021 cgtcggtttc tgtttcggcg ctttccttga aggagccgca ggctttggcg caccggtagc
    13081 aattaccgcc gcattgctgg tcggcctggg ttttaaaccg ctgtacgccg ccggactgtg
    13141 cctgattgtt aacaccgcgc cagtggcatt tggcgcgatg ggcattccaa tcctggttgc
    13201 cggacaagta acaggcatcg acagctttga gattggccag atggtggggc ggcagctacc
    13261 gtttatgacc attatcgtgc tgttctggat catggcgatt atggacggct ggcgcggtat
    13321 caaggagacg tggcctgcgg tcgtggttgc gggcggctcg tttgccatcg ctcagtacct
    13381 tagctctaac ttcattgggc cggagctgcc ggacattatc tcttcgctgg tatcactgct
    13441 ctgcctgacg ctgttcctca aacgctggca gccagtgcgc gtattccgtt ttggtgattt
    13501 gggggcgtca aagattgata tgacgctggc ccacaccggt tacactgcgg gtcaggtgtt
    13561 acgtgcctgg acaccgttcc tgttcctgac agctaccgta acactgtgga gcatcccgcc
    13621 gtttaaagcc ctgttcgcat cgggtggtgc gctgtatgag tgggtgatca acattccggt
    13681 gccgtacctc gataaactgg ttgcccgtat gccaccagtg gtcagcgagg ctacagccta
    13741 tgccgccgtg tttaagtttg actggttctc tgccaccggc accgccattc tgtttgctgc
    13801 actgctctcg attgtctggc tgaagatgaa accgtctgac gctatcagca ccttcggcag
    13861 cacgctgaaa gaactggctc tacccatcta ctccatcggc atggtgctgg cgttcgcctt
    13921 tatctcgaac tattccggac tgtcatcaac actggcactg gcactggcgc acaccggtca
    13981 tgcattcacc ttcttctcgc cgttcctcgg ctggctgggg gtattcctga ccgggtcgga
    14041 tacctcatct aacgccctgt tcgccgcgct gcaagccacc gcagcacaac aaattggcgt
    14101 ctctgatctg ttgctggttg ccgccaatac caccggtggc gtcaccggta agatgatctc
    14161 cccgcaatct atcgctatcg cctgtgcggc ggtaggcctg gtgggcaaag agtctgattt
    14221 gttccgcttt actgtcaaac acagcctgat cttcacctgt atggtgggcg tgatcaccac
    14281 gcttcaggct tatgtcttaa cgtggatgat tccttaatga ttgttttacc cagacgcctg
    14341 tcagacgagg ttgccgatcg tgtgcgggcg ctgattgatg aaaaaaacct ggaagcgggc
    14401 atgaagttgc ccgctgagcg ccaactggcg atgcaactcg gcgtatcacg taattcactg
    14461 cgcgaggcgc tggcaaaact ggtgagcgaa ggcgtgctgc tcagtcgacg cggcggcggg
    14521 acgtttattc gctggcgtca tgacacatgg tcggagcaaa acatcgtcca gccgctaaaa
    14581 acgctgatgg ccgatgatcc ggattacagt ttcgatattc tggaagcccg ctacgccatt
    14641 gaagccagca ccgcatggca tgcggcaatg cgcgccacac ctggcgacaa agaaaagatt
    14701 cagctttgct ttgaagcaac gctaagtgaa gacccggata tcgcctcaca agcggacgtt
    14761 cgttttcatc tggcgattgc cgaagcctca cataacatcg tgctgctgca aaccatgcgc
    14821 ggtttcttcg atgtcctgca atcctcagtg aagcatagcc gtcagcggat gtatctggtg
    14881 ccaccggttt tttcacaact gaccgaacaa catcaggctg tcattgacgc catttttgct
    14941 ggtgatgccg acggggcgcg taaagcaatg atggcgcacc ttagttttgt tcacaccacc
    15001 atgaaacgat tcgatgaaga tcaggctcgc cacgcacgga ttacccgcct gcccggtgag
    15061 cataatgagc attcgaggga gaaaaacgca tgattatttc cgcagccagc gattatcgcg
    15121 ccgcagcgca acgcattctg ccgccgttcc tgttccacta tatggatggt ggtgcatatt
    15181 ctgaatacac gctgcgccgc aacgtggaag atttgtcaga agtggcactg cgccagcgta
    15241 ttctgaaaaa catgtccgac ttaagcctgg aaacgacgct gtttaatgag aaattgtcga
    15301 tgccggtagc actggcaccg gtgggtttgt gtggcatgta tgcgcgtcgt ggcgaagttc
    15361 aggcagccaa agcggcggac gcgcatggca ttccgtttac tctctcgacg gtttccgttt
    15421 gcccgattga agaagtcgcg ccagccatca agcgcccaat gtggttccag ctttatgtac
    15481 tgcgcgatcg cggctttatg cgtaacgcgc tggagcgagc aaaagcagcg ggttgttcga
    15541 cgctggtttt caccgtggat atgccgacac cgggcgcacg ctaccgtgat gcgcattcag
    15601 gtatgagcgg cccaaatgcg gcaatgcgcc gctacttgca ggcggtgacg catccgcaat
    15661 gggcgtggga tgtgggcctg aacggtcgtc cacatgattt aggtaatatc tcagcttatc
    15721 tcggcaaacc gaccggactg gaagattaca tcggctggct ggggaataac ttcgatccgt
    15781 ccatctcatg gaaagacctt gagtggatcc gcgatttctg ggatggcccg atggtgatca
    15841 aagggatcct cgatccggaa gatgcgcgcg atgcagtacg ttttggtgct gatggaattg
    15901 tggtttctaa ccacggtggc cgccagctgg acggtgtact ctcttccgct cgtgcgttgc
    15961 ccgctattgc agatgcggtg aaaggtgata tcgccattct ggcggatagc ggaattcgta
    16021 acgggcttga tgtcgtgcgt atgattgcgc tcggtgccga caccgtactg ctgggtcgtg
    16081 ctttcctgta tgcgctggca acagcgggcc aggcgggtgt agctaacctg ctaaatctga
    16141 tcgaaaaaga gatgaaagtg gcgatgacgc tgactggcgc gaaatcgatc agtgaaatta
    16201 cgcaagattc gctggtgcag gggctgggta aagagttgcc tacggcactg gctccgatgg
    16261 cgaaagggaa tgcggcatag tcgtttgcgc cccctcaccc taaccctctc cctcagggag
    16321 aggggaccgt tcggcgctgt atgtactccc tcactctgaa acgacaccgc actctttttt
    16381 tctccctcgc ccctccgggg agggccgggg tgaggggaaa aggccgcacg gttcaccaat
    16441 tttctgctat cctgcccccg cactaagggg gcagtatgct aaacatcgta ctttacgaac
    16501 cagaaattcc gccaaatact ggcaacatca tccgtctttg cgctaatacc ggctttcgtc
    16561 tgcatatcat cgaaccgatg ggatttgcct gggacgataa gcgcctgcgc cgcgcggggc
    16621 tggactatca cgagtttacc gccgttacgc gtcatcatga ctatcgcgcg ttcctcgaag
    16681 cagaaaatcc ccagcgcctg tttgccctca ccacgaaagg tacgcctgct cacagcgccg
    16741 taagctatca ggatggcgat tatctgatgt tcggcccgga aacacgcggc ctgccagcga
    16801 gcattcttga tgccctgccc gctgaacaaa aaattcgcat tccgatggtg ccggacagcc
    16861 gcagcatgaa tctgtccaat gcggtgtcgg tagtggtgta tgaagcctgg cggcagctgg
    16921 gatatccggg agcgttgttg agagattagt tactgtatgc cggatagatg attacatcgc
    16981 atccggcacg atcacaggac attagatccc atccccatac tcaaatgtat ggttaatacc
    17041 gttgaaatgc tggtccatat ccattgatgg cttatcgctg tctggtttac cgacaatacg
    17101 agccggaacg ccagcggcgg ttgtatgcgg cggcaccggt tgcagcacca cggaacctgc
    17161 gccaatcttc gcgccgcgac caacttcaat attgccgagg attttcgcgc ccgcgccaat
    17221 catcacacct tcacgaattt tcgggtgacg gtcaccacca gatttacccg taccgccaag
    17281 cgtcacagat tgcagaatcg atacgtcgtt ttcaattacc gccgtttcac ccacaacgat
    17341 gcccgtggcg tggtcgagca taatgccacg gccaattttt gctgccgggt gaatatcgac
    17401 ctggaacgtc acagaaacct ggttttgcag aaagattgcc aatgcgcgac gcccctgatt
    17461 ccacaaccag tgaccgatgc gataggcctg caaggcatga aaacccttca ggtataacaa
    17521 cggggttgag tatttatcga ctgccgggtc gcgggtacgc accgcctgaa tatcacaggc
    17581 cgcagaggcg atcatttccg ggtcagcggc gtaggcttct tccaccactt cacggatagc
    17641 aatagcaggc ataattggcg atgacagctt gttcgccagc atgtagctca gtgcactacc
    17701 aaggttttcg tgcttgagta gcgtcgcgtg gtaaaaactg gccagcattg gctcacagtc
    17761 cgccagcgtt ctggcttcgg ctttaatatt gttccagaca atttccagtt cttcacacga
    17821 cattgcttac tccacacgat gagataatga ccggcccgtt ctgcgcgggc cgggtcatag
    17881 cggtaacaaa ggttccctgg ggttagtggc tgctgcgctc gtccttgcgt gcacgaccta
    17941 ataacgtcaa tgctgcctcg cgcgcgtttt ttccgcaata taatacttga taaatttcct
    18001 cggttattgg catttctacg ccgaagcgat gcgccagttc gcggacttct ttcgtattgc
    18061 ggtagccttc caccacctga ccaatcttct cctgcgcgct ttgtacatcc atgccctgac
    18121 cgagcatcat gccaaaacgg cggttacgcg actggttgtc ggtacaggta agcaccagat
    18181 cgcccagccc cgccatgccc ataaaggtgg caggatcggc acccagcgcc gcgccaagac
    18241 gcgacatttc agccagccca cgggtgatca gtgccgtacg cgcattcgca ccaaaaccga
    18301 taccgtcgga catccccgcg ccaatggcaa tgacgttttt caccgcaccg ccaagctgca
    18361 caccgatgaa atccgggttg ctgtaaacgc ggaaactttt gccacagtgc agcaattgtt
    18421 ggagatcatc ggcaaaggtc tggtcggtag aggccaacga aatagccgtc ggtaaacctg
    18481 ccgccagttc tttcgcaaac gttgggccag agataaccgc cagcggaatt tgatcgccta
    18541 aggcctcacg cgccacgtcc tgtaacagac gtccggtttc cgcttccagc cctttggtcg
    18601 cccacaccag acgcgcatca ggacgcatca gcggtttaat ctggcgcagc acttcaccaa
    18661 agacatggct gggtacgacg acgagaatat tacggctggc tgccagcgca gtggcgagat
    18721 cgctttcaag atggagcgta tcgggaaaag gcacatcggg gagaaacgcg gcgttacagc
    18781 ggtcgcgttc aagcgttgcg atatgttcag ggtcatggcc ccagaggaca acctcgtggc
    18841 catttcttgc cagggtaatg gcaagagcgg tgccgtacga gccggcaccg atcacagtca
    18901 ttgaagcatt acgttggttc atcaggcatc ctgatgttct tcagtacctt cgccagcctg
    18961 ctgctgcaaa tagttcatga acagcgcatc gaagttaatc ggcgcaaggt tcagttgcgg
    19021 gaatgtaccg cgggatacca cgctggtgat gcactcacga gcatacggga acagaatgtt
    19081 cgggcagtat gcgcccaggc aatgtgccat ctgggtgcct tcgatacccg cgatggagaa
    19141 aataccgccc tgctgaactt cacacaggaa cgcggtttct tcgcccaggg aggccgttac
    19201 ggtaacacgc agtaccactt cgtatacgtc atctgccagt tgggaagatg ccgtatccag
    19261 atcaagttta acttctggtt gccaatcttt ctggaaaacg tgcggcgcgt tcggcgcttc
    19321 gaaagagata tccttggtat aaatacgttg gatctggaaa gtcatttcag tgttgttttg
    19381 ttctgacatg tgtagaaaac ccttaagtgt tgtccttaaa tactgcgcaa tgtaatgcca
    19441 acgttcacac gttatttcag caggggatcc agtccaccac gtgcatccaa tgcatacaag
    19501 tcatcacagc cgccaatgtg ctgtgcgtca ataaaaatct ggggaaccgt ggtgcgaccg
    19561 ctgcgtttga tcatctcttc acgcttggcg gcattgccat cgatcggcaa ctcctgaaaa
    19621 ctcacgccct tgctgctcag cagtgctttt gcacgatggc aatacgggca ggtttctttg
    19681 gtatagattt caacattggc cataatttag ctcctgtttt tttaccctga aatttcatgt
    19741 tgcagggcgg ctgcaaaatg gagtgtcccc agaagcttat aacaataagt cgctgggggc
    19801 ctaattgcag ctaacggcct gcatcatgaa agacgacagg taaattattt gccgcgcacc
    19861 agaggcaagt tttcgccagc ccagccagca acgccctctt tcagcacgaa tacttgcgca
    19921 aaaccagctt tcgtcagcgc gtttgcaggc tcctggcact gcatgccaga accgtctacc
    19981 acgataaccg gtttgtcttt gtgcttctca agctcaccaa cattgttggc tttgatttcg
    20041 ctcggcaaca gattgataga acctgcgata tggcctttac ggaagtcatc acgctgacgt
    20101 aaatccacaa ccacagcgtc ttctttgttg atcagacgcg tagcttcacc acgagtaatc
    20161 accttcactt tcgaggtcag gctcttaaac gtagtcacaa gaaccgccac cagtaacgcg
    20221 atccaggcga tactcagtat gggatgacgg ccaacaaatt gcataatttc ttgcatgggg
    20281 ggtaacaact cccgacgtag tgattaaaaa aaccaggaaa ggagtatacc tgcgcggtgc
    20341 ggcaaataca gccagcgcgt taactggaat gcaattttgc ggggcacgac gaaaaaaaag
    20401 cgcaaatctg gcatcggctt gccgcgagcg gtcgtatatt ttgatcttca gaggctattt
    20461 tatcgattca gctgtagtaa aattacgcaa attttgactc ttgagtatga ggttgtcgca
    20521 atgtcggttt ctaaaaaacc tatggtactg gtgattctgg atggatatgg ctatcgtgaa
    20581 gaacagcagg ataacgccat ttttagtgct aaaaccccgg taatggatgc actgtgggcc
    20641 aatcgtccgc ataccctgat cgacgcttcc ggtctggaag tcggtctgcc tgaccgtcag
    20701 atgggtaact ccgaagtagg ccacgttaac ctgggtgccg gccgcatcgt gtatcaggac
    20761 ctgactcgtc tggacgttga aatcaaagat cgcgctttct ttgctaatcc agtgctgact
    20821 ggcgcggtag ataaagcgaa aaacgcaggt aaagcagtac acattatggg tctgctctcc
    20881 gcaggcggcg tacacagcca cgaagatcac atcatggcga tggtagaact ggcagctgaa
    20941 cgcggcgcag aaaaaatcta cctgcacgca ttccttgacg gtcgcgacac cccgccgcgc
    21001 agtgctgaat cctcgctgaa aaaatttgaa gagaaatttg ctgcgctggg taaaggccgc
    21061 gtagcgtcca tcattggtcg ttactacgcg atggaccgcg ataaccgttg ggatcgcgtt
    21121 gaaaaagcct atgacctgct gactctggcg cagggtgagt ttcaggccga tactgccgtt
    21181 gctggtttgc aggcagctta tgctcgcgac gaaaacgacg agttcgtgaa agcgaccgtt
    21241 attcgtgcag aaggtcagcc agatgcggca atggaagacg gcgatgcgct gattttcatg
    21301 aacttccgtg ctgaccgcgc gcgtgaaatc actcgtgcat tcgtgaacgc tgatttcgat
    21361 ggcttcgcgc gtaagaaagt ggttaacgtc gatttcgtga tgctgaccga atacgccgct
    21421 gacatcaaaa ctgcggttgc ttacccaccc gcttccctgg ttaacacctt cggcgagtgg
    21481 atggcgaaaa acgacaaaac tcagttgcgt atttccgaaa ccgaaaaata tgcccacgtt
    21541 actttcttct tcaacggtgg cgtagaagag tcgttcaaag gcgaagatcg cattctgatc
    21601 aactcaccga aagtggctac ctacgatctg caaccggaaa tgagctccgc agagctgacc
    21661 gaaaaactgg ttgcggccat caagagcggc aaatacgaca ccattatctg taactatccg
    21721 aacggcgaca tggtaggtca caccggggtg atggaagcgg cggttaaagc ggttgaagca
    21781 ctggatcact gcgtggaaga agtcgcgaaa gcggttgagt ccgtgggtgg acaactgctg
    21841 atcaccgctg accacggtaa cgctgaacag atgcgcgatc cggcaacggg tcaggcacac
    21901 acggcacaca ccaacctgcc agttccgctg atttacgttg gtgataagaa cgtgaaagcg
    21961 gttgcaggcg gcaaactttc tgacatcgcg ccgaccatgt tgtcgctgat gggtatggaa
    22021 atcccgcaag agatgactgg taagccgctg ttcatcgtgg aataatccct ccccatgagg
    22081 ggaaaggcga ttaataccat gacacgggcc gtgaaaccgc gcaggtttgc aatcaggccc
    22141 atcatctacg ccagcgttct tagcgctggc gtattgttgt gcgccttttc cgcccacgcg
    22201 gatgagcgtg accaactcaa atctattcag gccgatatcg ccacaaaaga gcgcgcggta
    22261 cgccaaaagc aacaacaacg cgcaagcctg ctcgcacaat tgaaaaagca ggaagaagcg
    22321 atctctgaag ccacccgtaa gctgcgcgaa acgcaaaaca cgctcaatca actcaataaa
    22381 cagattgatg agatgaacgc gtcgattgcc aaactggagc agcaaaaagc cgcccaggag
    22441 cgcagcctcg ccgcacaact ggatgccgca ttccgtcagg gcgagcatac cggtattcag
    22501 ctaattctca gcggtgaaga aagccagcgt ggacagcgtt tacaggctta tttcggctat
    22561 ctcaaccagg cgcgacaaga aaccattgcc cagttgaagc aaacgcgtga agaagtcgcc
    22621 atgcagcgtg ctgaactgga agagaaacaa agcgagcaac aaacgctgtt atatgaacag
    22681 cgcgcccaac aggcgaaact gactcaggct ctgaacgagc gtaaaaagac gctggcgggg
    22741 ctggaatctt ccatccagca aggtcagcag cagctaagtg aactgcgtgc caacgaatcc
    22801 cgtctgcgta acagtattgc ccgtgcggaa gccgcggcga aagcgcgtgc tgaacgagaa
    22861 gcgcgtgagg ctcaggcagt tcgcgacagg cagaaagaag cgacgcgcaa aggcaccacc
    22921 tacaaaccga ccgaaagcga aaaatcgctg atgtcccgaa ctggtggcct gggtgcgccg
    22981 cgtggtcagg cattctggcc agttcgtggc cctacgctgc atcgctatgg cgagcagcta
    23041 cagggtgaat tacgctggaa aggaatggtt atcggtgcct ctgaaggtac tgaagttaaa
    23101 gcaattgccg acggtcgggt gattctggct gactggctgc aaggctacgg tctggtggtg
    23161 gtggttgagc acggtaaagg cgatatgagt ctttacggct ataatcaaag cgcgctggtg
    23221 agcgttggtt cgcaggttcg tgcgggccag ccaattgcac tggtgggcag cagtggcggt
    23281 cagggcagac cttcactcta tttcgaaatt cgccgccagg gtcaggccgt caatccacag
    23341 ccgtggttgg gaagataagt tttgtttcca tttcgtcgta acgttcttgc atttgccgct
    23401 ctgttggcgc tctcctcccc tgtaattgct ggcaaacttg ccatcgtcat tgatgatttt
    23461 gggtatcgcc cgaacaacga aaaccaggtg ctggcgatgc cttccgctat ctccgtcgct
    23521 gtattacccg attcaccgca cgccagagag atggcgacca aagcgcataa cagcggtcac
    23581 gaagtgttga ttcatctccc gatggcaccg ttgagtaaac agccgctgga gaaaaatacg
    23641 ctgcgcccgg agatgagcag cgacgaaatt gagcgcatta ttcgtagtgc ggtcaataac
    23701 gtgccctatg ccgtggggat caataaccac atgggcagca agatgacctc taacctgttt
    23761 ggtatgcaga aagtgatgca ggcgctggag cgttataatc tttacttcct cgacagcgta
    23821 accattggta atacccaggc gatgcgcgcc gcgcaaggca ctggcgtgaa ggtgatcaaa
    23881 cggaaggtgt tcctcgacga ttcacagaac gaagcggaca tccgtgtgca attcaatcgc
    23941 gcaattgacc tagcgcgtcg caacggatca accattgcca ttgggcatcc tcacccttca
    24001 acggtacgcg tgttgcaaca gatggtttat aacctgccgc cagacattac gctggtgaaa
    24061 gccagcagct tgctgaatga accgcaggtt gatacgtcta caccgccgaa aaacactgtg
    24121 cccgacacac cgcgtaatcc gttccgtggc gtgaagctgt gcaaaccgaa gaaaccgata
    24181 gaacctgttt atgctaatcg cttctttgaa gtattaagcg aaagcatcag ccagagcacg
    24241 ctgatcgttt acttccagca tcagtggcaa ggctggggca aacagcctga agcggcgaag
    24301 cttaacgcta gcgtaaatta aaggcgctac gcgtaatgcg atggtgcgac cgcgttttgt
    24361 cgcgccattg ccataagcgg aaagactaca gtaacgcctg ataacccacc tttacgctgc
    24421 gcacattggt aatcaggcgc ttataca
//
LOCUS       DAETQN010000061        23480 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.061, whole genome shotgun sequence.
ACCESSION   DAETQN010000061 DAETQN010000000
VERSION     DAETQN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.061"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..157)
                     /gene="cas6e"
                     /locus_tag="K0W68_002405"
     CDS             complement(<1..157)
                     /gene="cas6e"
                     /locus_tag="K0W68_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HBI3073942.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREEL"
     gene            complement(139..885)
                     /gene="cas5e"
                     /locus_tag="K0W68_002406"
     CDS             complement(139..885)
                     /gene="cas5e"
                     /locus_tag="K0W68_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HBI3073943.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="K0W68_002407"
     CDS             complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="K0W68_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HBI3073944.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1963..2499)
                     /gene="casB"
                     /locus_tag="K0W68_002408"
     CDS             complement(1963..2499)
                     /gene="casB"
                     /locus_tag="K0W68_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HBI3073945.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2496..4057)
                     /gene="casA"
                     /locus_tag="K0W68_002409"
                     /pseudo
     CDS             complement(2496..4057)
                     /gene="casA"
                     /locus_tag="K0W68_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            complement(4155..6854)
                     /locus_tag="K0W68_002410"
     CDS             complement(4155..6854)
                     /locus_tag="K0W68_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBI3073946.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGWPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            complement(7049..7201)
                     /locus_tag="K0W68_002411"
     CDS             complement(7049..7201)
                     /locus_tag="K0W68_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBI3073947.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(7466..8200)
                     /gene="cysH"
                     /locus_tag="K0W68_002412"
     CDS             complement(7466..8200)
                     /gene="cysH"
                     /locus_tag="K0W68_002412"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBI3073948.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(8275..9987)
                     /gene="cysI"
                     /locus_tag="K0W68_002413"
     CDS             complement(8275..9987)
                     /gene="cysI"
                     /locus_tag="K0W68_002413"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBI3073949.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(9987..11786)
                     /gene="cysJ"
                     /locus_tag="K0W68_002414"
     CDS             complement(9987..11786)
                     /gene="cysJ"
                     /locus_tag="K0W68_002414"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBI3073950.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            12105..12467
                     /gene="queD"
                     /locus_tag="K0W68_002415"
     CDS             12105..12467
                     /gene="queD"
                     /locus_tag="K0W68_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBI3073951.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            12545..13816
                     /locus_tag="K0W68_002416"
     CDS             12545..13816
                     /locus_tag="K0W68_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI3073952.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            13807..14067
                     /locus_tag="K0W68_002417"
     CDS             13807..14067
                     /locus_tag="K0W68_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBI3073953.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            14084..14659
                     /locus_tag="K0W68_002418"
     CDS             14084..14659
                     /locus_tag="K0W68_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HBI3073954.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(14807..15667)
                     /locus_tag="K0W68_002419"
     CDS             complement(14807..15667)
                     /locus_tag="K0W68_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBI3073955.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(15664..16443)
                     /locus_tag="K0W68_002420"
     CDS             complement(15664..16443)
                     /locus_tag="K0W68_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBI3073956.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(16421..17830)
                     /locus_tag="K0W68_002421"
     CDS             complement(16421..17830)
                     /locus_tag="K0W68_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3073957.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(17852..19306)
                     /locus_tag="K0W68_002422"
     CDS             complement(17852..19306)
                     /locus_tag="K0W68_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBI3073958.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(19376..20192)
                     /locus_tag="K0W68_002423"
                     /pseudo
     CDS             complement(19376..20192)
                     /locus_tag="K0W68_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            20510..21787
                     /locus_tag="K0W68_002424"
     CDS             20510..21787
                     /locus_tag="K0W68_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3073959.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            21814..23299
                     /locus_tag="K0W68_002425"
                     /pseudo
     CDS             21814..23299
                     /locus_tag="K0W68_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         5632 a         6227 c         5791 g         5830 t
ORIGIN      
        1 ggagttcttc ccgacgataa agaaattgcc tttctttgcc gccagggaaa agatcccaca
       61 gccactgatg catcacatat tcaccgcgat ccaccagatg cagcaactgc gcaggcgaaa
      121 gctggccggt atgtaaggtt attcttgaga ggtacatggt tcctccttgc tgagccacgg
      181 cccctgattg acggtgcgtt ccccaaacag ccactgctga cgatttaaag gaacatcccg
      241 gcggcgtaat attttgctcg cgaccaggcc gtcgtgtttc ccttcccacc agcattcatc
      301 ctgaagtttc gggagtgaga ctttcagttt atggaaatga tcctgatact gttggtatgc
      361 attacataag gcatcacacg cgttgccttc gagcagtaac ggcgcaagag gtaatgccag
      421 cggatgactt tttcgcccca gataaagcgg aaaaactgga tggcgtaaac cgtcctgcaa
      481 ctgttcgagg ctgtaaggcg catcggctgt tgttgccacc gccaccatcc accaggcatc
      541 ggtgtagtag tcgcgccggg agataatcgc gctcaaaagc tcagggtcgc tcaactcttc
      601 acgacggctg aaataacgtg ctttacgcac ctcttttggc atctggaccg tgtgataatc
      661 ccgtgcccag cgcgggttac ggctggcgca aaccaccagt gaatagtggc gattaaacgc
      721 gtttaagcgt tcggtatcat cacgccgaat ccctaccccg gcagccagta gccccagcaa
      781 tgctgagcgc gaaggaagtt catgggtatg acgaacttca cctggagcat cgacacccca
      841 ggacgccatc ggcccatgaa gctgaaaaat cagatattgg ctcataagcc gccccttacg
      901 cgcagataaa gtccagcacg tccttcatgc ttccctgttt gttcatcacg tcaaaacttg
      961 cgcattcggt cttctgttcg tagaccgtat tcatgttttc gcgaagcgtt gtaatacgct
     1021 gcaccgccac atctaactga cgtgtgccat taatgggttc atagaaagcc gccgccagag
     1081 agcgtggttg atcggtgcct ttttctgcca gcgcccagga agcataggca cggctggcaa
     1141 agctattttg tttaccggtt ggggaaactt taagcgcggc ttccgtaaaa gcgcgtatgg
     1201 tctgattagc taacgcttcg tcaccgccga ggttttctac cagcagatct ttatcaatgc
     1261 agatataggt gtagaaaagt gctgaaccga atccggtttc tccgagatgc ccggcaccgg
     1321 catcttcaga ggcctggcgc aaatcatcaa cggcggtgaa aaaatcatct tcgacaatcg
     1381 tttcactgac accaaacgca tgcgcgacct ggcaggcggc ttcaacatta aactcaggtt
     1441 tattcgccag catacgacca aacatagcga tatctaccgc tatacggtct ttacgtaaca
     1501 acgcgagatc ttcctctttt ggcgcgcgct tttcttctgc cagttgatga gcaagcgctt
     1561 ttacggcgtc aaattctgcc gggctgatat gaactaattg ttcagtttcg gcgttagtga
     1621 gcggatcttt tggttttttg tcatttttag ctttcccaag ataatcagca atttttgccg
     1681 cccattcgat ggctttttta tcttcaatcc ctttctcaat caggatagtt gccgcctcac
     1741 gcgcaatacg cccactgcga ataccaatat ggcccgccag tgcctgttca aaaagtgcag
     1801 aagtgcgcca cgcacgtttc agactttgcg aggaaacgcg cagacgcgtt gctccaccca
     1861 gcaccacagt ttttggcgca ccggtgtcat cacggttaag gttggcagca gggtaagcgg
     1921 ttaacacatg aagctgaata aatgttgtca tgagatagtc ctttattgat tttgttcgtt
     1981 atcggcgtcg ccagcctggt aatattccag cgcccagcgg atacggataa attcggttgg
     2041 ccgctgctgg cggcggtgat gattgagcag gtcgtcgctc tcctggcacc agcggaaaat
     2101 gccatctgcc agggagtcaa gattgacgga accgtttaat aatctgacgg cgcggcgtaa
     2161 ctggcgcagt aattcatccg gtgtttttac tgctgacagg cgagtaaaac gcccctttga
     2221 cattaccgcc gccagttgcg cggcaaaagg ctgccgttca tcaatggctt taacattggc
     2281 gctaactgcg gctatcagcg ccagtgcggt gatacgccac tcaggttcat cctgccactt
     2341 tatttgtctg ttcttcagaa acaggcgaaa tccatccgtc agacaaacat cattaaccgt
     2401 cgtactacgc cgcagactgg cacgttcgcc gcgtttctcc tgcaattcct cgtgccattt
     2461 gcgcagcgtg gctttgtgct cctcttttac aatactcatt cagcagcctc ctgctttttc
     2521 tccctggcgg ctttagcact ttgcttctcc gccgatgttg taaaatattt cttgcgcgcg
     2581 gtcatgatgc gttccaaatc aacgggctca taaggattgg tgaatacacg ctcgtcaaaa
     2641 tcctgacgcg cgaataacca aatttcctta ttccatttgc tgagtaattc atccgcatcc
     2701 tgaccttctt caatttggcg cactaacctc aggaagcgat gctgagtttt gttccagaag
     2761 tcgatatcca caaaactaaa atcacccctt gcaccttttg gatcggagaa ccatgcttct
     2821 ttcaatgcac tccgtaacag acttagaatc cgtgaagccg tttgcgcagc cagccgcagt
     2881 ttcggtatct ggccttcttt ttattgagca gcagcgggaa atgatgttcg taccaacagc
     2941 gcgctttcat gttgtcgaaa tcatagccaa atccccacag gcccactttt gcctgcttga
     3001 gactgctggc attaaagagt ttcaccacca gcgcgggaag ttccgtattg ttttctgact
     3061 tacccgtttc gataaggcct aaccagtcgc gccagattaa accgcccggt tgtggtttga
     3121 ccgagtaaaa ctcgccgccc tctttaagta gtacacggta aggcgttaag ggatgctgcc
     3181 acatggcata gttagcgccg taatttttgg tcgtcatcag gctcagaagc gcgtcgctct
     3241 gctcaccgca aatatcgcag ttgccgactg tggtggtatt aaaatcaata cgaatacggc
     3301 gaggcattcc ccagtacgcc tggagtttat tgacctgctc atcggttacc accgcaccgg
     3361 ccagttcgct gatgcgcgtc gggccaagcc aggggaaaac cagatcgtca aattttttgg
     3421 gtagcggtaa atcggcttca tcctgcggca tgacattgat ccacagtttg cgccacaagg
     3481 gagtttgttg attaccctga tattcctgta attcaatcag agtggtcatc ggcccaccgc
     3541 cgcgtaaacc ggtgcggtag cctttgccac ctgacggcgc attcaactgt agggagaaca
     3601 gagctaacgc agagcaatgt gggcacaggt attccgttac accacgcttg ataaagtggt
     3661 ctttattaaa cttcgttgtt tgagcgccgg gaatctcagg cagtagcgaa gcgaccggaa
     3721 ctttatctcc ggtgagcgcc tcgaaatcct gtataaatga aggggaatct ggcccaaact
     3781 ggaaagcatg ttctaatgac agcaatgctt cccgtagctt ttcagcttcc agtccgtctt
     3841 cccagatatc atcccaacga cgatgatctt ttggcgcgaa gctggtttgt agtagcccca
     3901 gcaaaaactg ccataccgca ccctggagat ctgcccgcgg cgcagaaata tcgacaacat
     3961 tttcatccgc cagatcgact ggcgccagtt tgcctgtcgt tccgtcttta aaacgaacgg
     4021 gcaaccacgg ggttgtcaga agtgaaaacg agttcatacg catcctctcg taaaaaagac
     4081 catcccaggt cggttgtcca tctttccatc cttttcggtg tatcaactat tcagagagtc
     4141 ctgtcgcaga catctcaacc aaccttgcca atcaatcctt ccgtagccga atacccacaa
     4201 ctttcgtcat cgttgactaa aataacgttt gccatctccg gatcttgtcg ctgttcaatg
     4261 caccacagcc tgaacgcctc tccctccagt aaagaaaact catcccgatg ttttttccac
     4321 cagcttcggc gcactctgac aacgctcatt tcccatgcgt gagcaccggt ggcataaggc
     4381 ttcaccacac cgtcaataca ggtagccagc cacagggaaa cagactcctc agccagacgt
     4441 gtcgacagtt tttctgggag gtaatcgttg atattggcgg catagccggg cctgaagttc
     4501 agcacaaact ttttagccat tgcgcgatca cagtaatatt tgcccacttg ctcctgctcg
     4561 ctgcgggcaa atccttccgg cattaccacg tcctcaccgt agacggattc aataagaagc
     4621 cgggctgcgt gtggcatctg aatagcacct tgctcacgca gcacacgctg cgtcagccag
     4681 attcgcccat gatcgggata gacaaatgca ctgttacgca tggcactgcc aaaccattcg
     4741 tcgccaggag agtcgtccca gacgggagcc agaatcagca attcaggggg ggaacgctcg
     4801 tcctttccgt cacgctttaa ctgaccatta atatcgcgga tatgccgctg taatcgtcct
     4861 gctcgctgaa tcagcaaatc aacaggggcc aggtcggaga tcatttcgtc caggtcacaa
     4921 tcgacactct gctctaatac ctgagtacaa ataaggactt ttccggcacg ctgtgagcta
     4981 tcttctttac caaagcgtgc cagtgtctcc gtttcaattc gctggcgatc gctaaaagca
     5041 aagcggctat gaaaaagtga aaggctggaa gcaggaatga cgccgcgggc aagcagctga
     5101 cgatgaacct taatagcgtc atcgacagaa ttccggatcc aggcgatgca tttcccctga
     5161 cttaccgccg attcgatacg cgcaatacac tcttgttcac tatgaagcca acccacgctg
     5221 acgctacgct caacgtcttt gcgcgtcgct acccggtgtg agttcacatc ggatttcgtg
     5281 acatgcgtca gccaggggta atcatccttt tccaggaacg gagcttcttg caggccctct
     5341 gtaccccgcg caaaggcagc gacgagtttg tcgcgctgct gttgagataa cgtggcagaa
     5401 agcaaaataa cgctgtttcc gccacgcgcc tgccgctcga tcaacccttc aagaatgcac
     5461 gacatgtaag cgtcacaggc gtggatctca tcagccagca ggattttgtt actcagcccc
     5521 agaagccgca gattattatg tttgaacggc atcaccgcca tcatcgcctg atccagcgtg
     5581 ccgacgccaa tttcagccag caacgccttc ttgttactgt cggcaaacca ggccgcacat
     5641 ccctgactga acgtttgttc atccggttct tctgacccga ctaaatcacc agaccagagt
     5701 gattcattaa agcggtccat taatgtgcgg gcactatgtg ccagcaccag gctggggcgg
     5761 gattctggcg aatagaaagc aagccaggtt ttgaccagcc gatcgtacat ggcattggcc
     5821 gttgccattg ttggcagacc aaaaaacagc ccctgagcct tccccgcagc tatcaacctg
     5881 tgcgccagga taagcgccgc ttctgtttta cctgcgccag tcacatcttc cagaataaat
     5941 aactgcggtc cttgctggct gatatccaga tccagcacct tttgctgtaa cggtgtcgga
     6001 ttctcaataa aaggaaacag cgtattaatt ccgttgaaag gcgcgacttt tgcttttaga
     6061 ggaaagacgg ttaaggcgtt ttgagcctga actaaagttt tctgccagta acctttaata
     6121 tccattgggt gtgcgacgcg tggaaaaaat cgcgttgacg aacccgtcca gtctgcgagc
     6181 acgacggttg cagagatata ccaggaaagg tgttttataa gttcaatgcc ctcatcatca
     6241 tcccagaatg cggggatctc tatgagcgga aacagtggtt tgatttcaag gagaaaatct
     6301 cgtgctgcag ctttgtcttc aggcagaaaa ttatccaatt catccatacg atcagggggc
     6361 caaccatgat gccctgtcgt cacgggcatc cacatttcta tcactcgcct aagtttgcgc
     6421 ggagagagcg aagatgaagg aagtaactcc tgacattcac ttaaataatg attccacaac
     6481 cagtagccca gcgttgaatg agagattttt tcgtaatttt ttctggcacc ttcctgaatc
     6541 ttgagttcag ggggcaggta aagttgttga aaagagcggg caaattttcc aatatcgtgc
     6601 cagcacagca accaggcgaa aaattgagcc gcctgttcct tgtcagaaat ccctaattga
     6661 cgaaaataat cagccagccc gaagcaattt cttttaacca ttaaatagcc catcgcggct
     6721 acatccagcg aatgccagca aagaaggtga tagtcgtcgc ccccgtcttt ctcgccgcgt
     6781 cgggtttttc cccagaaatc aaaaaaagtc acaatatttt tatccttcag taaacttaaa
     6841 ggatatttac gcatatatta aaaattatct gtgatatata tcattcaata aaatatttac
     6901 attatgaata atcaaataat tttcatccgt aatatatatg aataataaac ataggcataa
     6961 tattcacata aataatataa attaaaaaag ggtgcaggag accaaaccat cacaattcag
     7021 tacgcggggt tttacccccg cgtggtggct acttcttcga ttcgtaagcg agaactgcct
     7081 taaactccat accacgttct ctgatactca actcacacaa cgagtcgcct accatcagcg
     7141 tgaatcccag tactgtaaaa cacagcacta tgattgccac aagggcatat tttgtcagca
     7201 tgtcttgcct ccctgcaaag aagagactaa cattcgaatt gtttgggttc gaacgctggc
     7261 ctcaggttga tagaaatatc gcctggggct tttgtccatc tggaacctcg cgaatgctta
     7321 acgccagaca gcctcaagca cccgacgcca ttctatacct gataattctt ccagcggcgt
     7381 tcacgtttaa aatcaggaat actgctcgca aaataaaaac gcccccatca tttctgacag
     7441 aggcgtttaa tttatccggc aatatttacc cttcgtgcaa tccacattcc ctttttaagc
     7501 caaagaaacg tgtttcttct tccgacatgc caggttccca tttacgggtt gtatgggtat
     7561 caccgaccga taaatatcct tcatcccata atgggtgata tttcaggcca tgtttttgca
     7621 ggtactgata aatagttcgg ttatcccagt cgataatcgg cagcacttta aatacgccac
     7681 gctgaattgc cagcaccggt aaattggcgc gactgccgga ttgttcacgg cgcaggccag
     7741 caaaccaggt ttgcgcgtta agttctttca gagcccggtt catcggttcg actttgttga
     7801 tgtcattgta cttttcaatg ccttcaacgc cctgctccca cagttttccg tagcgtgctt
     7861 cctgccaggc tgcgctttcg gtagcacggt acactttcag gttgagcttg agtttgtccg
     7921 ttaactcgtc aataaagcgg taggtttccg ggaacaagta acccgtatcg gtgaggatca
     7981 ccggaatatc cgggcgaatt tgattcacca gatgcaggct caccgccgcc tgaatgccaa
     8041 agctggaaga aagtacatat tcaccgggca gattatccag cgcccaggct acgcggcctt
     8101 cagcgtccag tttttccagt tcggcgttag tttccgccag cgccagaata cgatctacct
     8161 tcggcagttc gttcagggcg tttagatcga gtttggacat agattcctca ctgtttgcct
     8221 tgcctgatgc gacgctcacg cgttcttatc aggcctacaa gaccgggctg atggttaatc
     8281 ccacaaatcg cgcgccggat cgagcaccgg gcgaatgatg cccgcacgca ccgtaaagtc
     8341 gccgaagcct tcacccgctt cgcgctcttt cgcccagcgc cctatcagtt catcaagcga
     8401 cgccaggatt tccggctcgg tgatgttttc tttatacatc cgtgggatac gtgtcccaat
     8461 gcggttgccg ccaagatgca ggttgtagcg acccggcgct ttacccacca ggcccacttc
     8521 cgccagcatc gcgcgaccac aaccgttcgg gcagcctgtt acacgcatca cgatatgctc
     8581 atcgctgaca ccatgtttcg ccattaaatt atcgatgttg tcgataaaag acggcaggaa
     8641 acgctctgct tccgccatcg ccagcgggca agtcgggaat gacacgcagg ccatcgagtt
     8701 ttcacgctgc ggcgtgacgg cattcattaa cccgctctct ttggcgatct tctcgatctt
     8761 cgctttctcg ctttccggta caccggtgat gatcagattc tggttcgccg taatgcggaa
     8821 atcgcctttg tggatcttcg cgatctccag caggccggtt ttcagcggac gccccggata
     8881 atcaaggatg cgaccatttt cgataaacag cgtcaggtgc cagttatcat caatgccctt
     8941 aacccagcca atacgatcgc ctcgtccggt gaactcatat ggacggatcg gttcaaattt
     9001 gatccccgcg cgacgctcca cttccgcttt aaacgtctca acccccacgc gctccagcgt
     9061 gtatttggtt ttggcatttt tacgatcggt tcggttaccc cagtcacgct gagttgtcac
     9121 gacggcttcg gccaccgcca gcgtatgctc cagcggcaga tagccaaact cgctcgccgt
     9181 gcgggcgtag gttttcttgt tgccgtgttc gatagaaagc ccaccgccca ccagcaggtt
     9241 gaagcctacc agcttgccgt tttcggcgat tgccacgaag ttcatgtcgt tggcatgcag
     9301 atcgatatcg ttctgcggcg ggatcactac cgtggttttg aacttacgcg gcaggtaggt
     9361 ctgaccaagg atcggttctt catcagtagt ggcgactttt tcctggtcga gccagatctc
     9421 cgcatacgcg cgggtacgag gcaacagatg ctcagaaatc ttcttcgccc actcgtacgc
     9481 ttccgcgtgc agctgcgact cgtaagggtt cgaggtgcag agtacgttac ggttcatgtc
     9541 gttagctgtc gccagcgcat caagaccgac cgagtgcagc atctggtgca ccggtttgac
     9601 gttctttttc agaatgccgt ggaactgaaa cgtctggcgg ttggtcaggc gaatgctgcc
     9661 atagatggtg ttttcaccgg caaatttgtc gatcgcctgc cactgtttag tggtaatcac
     9721 cccacccggc agacgacagc gaagcagcat cgcgtggcgc ggctccagct tctgttcagc
     9781 acgttcggca cggatgtcgc ggtcatcctg ctgatacatg ccgtggaagc ggatcagcag
     9841 gaagttgtcg cccttaaagc cgccggtcag accgtcgttt aaatcttccg caatggtgcc
     9901 gcgcaggtag ttgctttcaa gcttcatgcg ctcggcgtct gtcagttttc cttcgaccac
     9961 taaaggccct ggatgttttt cgctcattag tagacatctc gctgataacg gcgctctacg
    10021 cgcagctcac ttaaaaattc atccgccgct tcggtgtcca tgccaccaaa ttcggcaatc
    10081 acttccagaa gtgcctgctc aacgtctttc gccatgcgat tagcgtcgcc gcagacataa
    10141 atgtgggcac cgtcattgat ccagcgccac agctccgcgc cctgttcgcg cagtttgtct
    10201 tgtacataaa ctttttcttt ttggtcgcgc gaccaggcaa gatcgatacg cgtcagcacg
    10261 ccctctttga cgtagcgctg ccactccacc tggtagagga aatcttccgt aaagtgcggg
    10321 ttgccaaaga acagccagtt tttacccggc gcttcgtcgg cggcgcgttg ctgcataaag
    10381 gcacggaacg gcgcgatgcc ggtaccgggg ccaatcataa tcactggcgt ttgcgggtta
    10441 gcgggcaggc ggaaattgtc gttatgttcg ataaatacgc ggacttcgcc ctcttcttcc
    10501 acgcgatccg cgaggaagct ggaggcacca ccggcacggg cgcggccttc cacgtcgtaa
    10561 cgcaccacac caacggtgac gtgtacttcg ttctcgactt ccgcctgcga ggaggcgatg
    10621 gaatacaggc gcggggtcag cgggcgcagc aggttaatca gcgcttcggc atccagttgc
    10681 gccggagaga aacgcaccat gtcgacaatc ggcgtggtcg cggcgtaatg ctgtaacttc
    10741 gctttatcgc ccaccagcgg cagcagcgtt tcgctgcggg taagcgtggc atagttctca
    10801 acaatgttgg cggtgttgac ggtcagttcg aagtgccact gtagcgcttc gttcagaggc
    10861 aacgttttgc cctcgacggt gacaggttca tcgcctttca gccacagcag ttcgacaagt
    10921 tctttcacca gtgccgggtc gttctgatac cagacaccca gcgcgtcacc cggctggtaa
    10981 cgcaggcctg aatcgcctaa gtcaatttcg atatgacgaa cgtctttttc agagttacgc
    11041 ccggtaattt tctggttaac cgaaaggctc gccaccagcg gcgcgtcttt gctgtacggg
    11101 ctggtgtgga tttcatttac cgcgccagta gcgacggatt gcgaaggtgc cgcgacaggc
    11161 gcacgcgatt taagcgcatc aaccacgcgg gcgcgccact cgctggcagc agcctggtat
    11221 tcaacatcgg catcgacacg gtcgagcagg cgttcaccac ccagttccgc cagcttgctg
    11281 tcgaaatctt tcccggactg gcagaaaaat tcataagagc tatcgccgag gctaaacacg
    11341 gcaaacgcgg tgttttccag ctttggcgct tttttggaga acaggaactt atgcagcgcg
    11401 acggcttctt ccggcggttc cccttcccct tgcgttgacg tcactacgat gagcagtttt
    11461 tcgctggcga tttgtttgaa tttatagtcg cccgcgttca ccagcttaac gttcagtttt
    11521 gctgctaata aatcatcgcg taatgcttca gcaacccggc gcgcattgcc ggtttgcgag
    11581 gcggagataa tagttatacc cggcatttct gcggctggcg ctggcgtcgc tgcaagcgca
    11641 gcaggctgct gattgagtac gccccagaaa tagccagaaa cccaggcaag ctgggtaggc
    11701 gttaaatcgg tcgtggccgc ctgaaggcgt gccagttgct ccgggttcaa cggaagcaac
    11761 gcggaaggtg ggacctgtgt cgtcatgcgt cgttatgttc cagtaagcaa agctgtttct
    11821 gcgccctgtc agcgcccata aaacagaaga gaagataagg ttaacggggt gaacggtgtg
    11881 gattaaagac gggatagcga taactaataa ccaaatcgac taacctgttt tagcaatagt
    11941 ctttaacaac aaaatagatt aaccaaccca atgaaaaaca aatgaattta gccaatcatt
    12001 aagataaatc agcgattttg cgcaacaaag tcgttttaga taatgcgaaa aaacagcctt
    12061 tccggtactc tacggcggtt ttatcgtcct gtagagaaat tatgatgtcc accacgttat
    12121 ttaaagattt caccttcgaa gccgctcacc gcttaccaca cgtcccggaa gggcataaat
    12181 gtggtcgcct gcacgggcat tcctttatgg tgcgactgga aattaccggg gaagtcgatc
    12241 cgcatacggg ctggattatc gatttcgctg aactgaaagc ggcgtttaag ccaacttatg
    12301 agcgcctcga tcaccattat ctcaatgata ttccaggtct ggaaaaccca accagcgaag
    12361 ttttagcaaa atggatttgg gatcaggtta aacccgttgt gccgctgtta agtgcggtga
    12421 tggtaaaaga aacctgcacc gcagggtgta tctatcgcgg cgaatgataa gagtgtgtcg
    12481 gcggtcaatt tcccttaagt aacgctatgt tagggtgttg tgttctggat atctggggca
    12541 tgacatggaa gacgactgcg acattattat tattggtgcc ggtattgcag gcaccgcttg
    12601 cgcgttacgc tgcgcgcgag cgggtttatc cgttttgtta ctggaacgcg ctgaaatccc
    12661 cggcagcaaa aatctttccg gcgggcggtt atatacccat gcactcgcgg aactcctccc
    12721 gcaatttcat ctgaccgcgc ctcttgaacg acgcatcact cacgaaagcc tttccctgtt
    12781 aacgcccgat ggcgtaacga cgttttccag cttacagccc ggcggtgaat cctggagtgt
    12841 attacgtgca cggttcgatc cgtggctggt tgccgaagcc gaaaaagaag gtgtcgaatg
    12901 catccccgga gcgacggtgg atgcactgta tgaagaaaac ggcagagtct gtggcgttat
    12961 ttgtggtgac gatattctcc gcgcccgtta tgtggtgctg gcagaaggtg ccaacagcgt
    13021 cctggctgaa cgtcacgggt tagtgactcg tcctgctggc gaagcgatgg cgttggggat
    13081 caaagaagtg ctgtcgctgg aaacatccgc tattgaagaa cgttttcatc tggagaataa
    13141 cgaaggcgca gcgttgctgt tcagcggcgg aatctgtgat gacttacccg gcggcgcatt
    13201 tctttatact aatcaacaaa cgctctcgtt agggattgtt tgcccgctct cttcccttac
    13261 gcaaagtcgt gttccggcaa gcgagctgct gactcgcttt aaagcgcatc cggcagtgcg
    13321 cccgcttatc aaaaacacgg aatcactgga gtatggtgcg catctggagc cagaaggtgg
    13381 cttgcacagt atgccggtgc aatacgccgg taacggctgg ctgctggtgg gcgatgcgtt
    13441 gcgcagttgc gtcaataccg gaatttccgt gcgcggcatg gatatggcgc tgactggcgc
    13501 acaggcggcg gcacaaacgc taataagcgc ctgccagcac cgcgagccgc aaaatctgtt
    13561 tgcgctttat catcacaacg tagagcgcag cctgctatgg gatgttctac aacgttatca
    13621 gcatgttccg gcgcttttgc aacgccctgg ctggtatcgg gcgtggcctg cgttaatgca
    13681 ggatatttcc cgcgatttat gggatcaggg tgataaacct gttccaccgc tgcgccagtt
    13741 attctggcgt catttacgtc gtcatggcct gtggcatctg gcgggcgatg ttatcaggag
    13801 tctgcgatgt ctgtagccca taatctctgg cgcgttgctg atgcgccgca cattgttccg
    13861 gctgactccg ttgagcgtca gatggtacaa cggttgatta acgcctgtcc ggcaggtctt
    13921 ttttcgctca caccggaagg tgacttacgc gttgattatc acggctgcct ggagtgtggc
    13981 acctgccgtt tgctgtgcga cgaatccaca ctacaacagt ggcgctatcc gccttccgga
    14041 ttcggcatca cctaccgctt tggataaata ataaggataa tttatgcccc tcttacacct
    14101 actccgccag aatccggtga ttgctgccgt taaagataat gccagcctgc aactggctat
    14161 cgattctgaa tgccagttta tttccgtgtt gtacggcaat atctgcacca tcagtaatat
    14221 cgttaaaaag attaagaacg ccgggaaata tgcttttatt catgttgatt tgctggaagg
    14281 cgcgtcaaat aaagaagtcg ttattcagtt tttgaaactg gtgaccgagg cggacggcat
    14341 tatcagtacc aaagcctcaa tgttgaaagc ggcaagagcg gaaggttttt tctgtattca
    14401 tcgcctgttt attgttgatt caatttcgtt tcacaacatt gataagcaag ttgcgcaatc
    14461 gaatccggat tgtattgaga tcctgccagg cggtatgccc aaagtgctgg gctgggtgac
    14521 agagaaaatc cgccaaccgc tgattgccgg tgggctggtg tgcgatgaag aagatgcgcg
    14581 taatgcgatt aacgcgggtg tcgtggcgct ttccaccacg aataccgggg tctggacgtt
    14641 agcgaaaaaa ttactttgac gggataatcg taaccaattg aatttggttt gatttgtagg
    14701 ccgcacgcca catccgacat tcagcgcctg atgcgacgct tgacgcgtct tatcaggcct
    14761 acaagtcccc aggccagaac cgtaggcagg atcatccggc aacacgttat tggcaatctg
    14821 cgtgaatgtt tgtcaccagt gcctccagca ccactttcca gtcatcaacc acgccaacat
    14881 ctgcctgtga aaataccgca gcgctggcgt catgattaat cgccaccaca aacttgctat
    14941 tgcgtacgcc cgccattaac gctgctgcgc cagaagcgcc aacaacaata cacacctcag
    15001 gtgccagcag atgtccggaa ataccaatca ctttttcagc atcaacgcca ccgttcatca
    15061 ctcgcgcccg actgtagccc acttcggctc ccagcttttc tgccagcata gcgatttcct
    15121 ggttatctgc ctctccgccc tgccccacca ccagcacccg tctggcctca gctaatgggt
    15181 cgcgagtaac atttttcaga tcctctgtgc taaccagcca gtccggaagc gcacccggga
    15241 caatgttcag ttgttgcatt ccagagggca acgtggcgtt tttcgctgct cccgcctgac
    15301 gcgccaggga aaggcatagc gggcgttttt ctgtttgcag cgtcgccgtt agcgcatttc
    15361 cccagtggga tttgcgcacg cttacggttg gaatatctaa cgaagtcacc tggcagatgc
    15421 ttgcaccatg taaacgccag gccagccggg tcgacagttc atcgccaaac gttcccggcg
    15481 gaaagagaac aacgtccgca ggcgttcgct gccactgttc caccaacgca tccagcacct
    15541 gttccgccac tacaggctga ggttcaatct gccagtgcgc cagggtacaa ccgctgaaat
    15601 cctgcgccgc cagccagctt gctattgcgg cattctcctg atttattgtc acaattgcga
    15661 tattcatggc tgcatccttt ggcgtaaata gtcctgccat agcttttgag ctttttctgc
    15721 taccgtttgc ccatcaatca gcgtcgctcc gcgtcgttgc tctgctcttg ccagttgcag
    15781 acactgcatc gctggcattt cggcagcaac agttttacgg ataatttctg ctttccccgc
    15841 agccattcgc tggcgcattc ccggtactgg caaggccact tcaccgcact gacgcacggc
    15901 aatcaccgca ggcaagcgaa cccggcaaca acgcaaccca tgttcagtac gctgttcgag
    15961 ggtgataaac agcgcgtcga gagtgaaacg ctccacctgg gtgaagcagg gccagcccag
    16021 catttccgcc agcaaaaatg gcgtttgccc attctgccct tcgctgcttt ggcagccggt
    16081 aattatcaga tccagcggat tctgatgctg ccattcggca atatggcgag cgacaaattc
    16141 cggcgcaaag cgcagatctg ccgccgtctc cagcaataca gcttcctcaa acccaagagc
    16201 catgagatag cgcagccagt gcaatgcccg ttcatccccc atgctcaacg cggttaaaga
    16261 catcggcgta ccgttttttc tctgcgccaa cagcagcgcg gcagcggcct gttcatcagc
    16321 accgagtaaa cttcgcagta gcgaaatatc cggtccgctt ttaccctgag ccgccgcctg
    16381 ccactctttt tccgccagca ttccggcatc cggttcggct ttaaacgcta acagaatgtt
    16441 cattcgcacc tcctacattt cccgccgcca ccagcgggag ggctttagtt tctggtgccc
    16501 atagccaggt cacaacaaaa tcaaccaaca acactgtcgc caggagcaat aaggtgactt
    16561 gcattcccca ctgcgccagc acccacggca gcaggccagt acttaccgcc gcgccaagtc
    16621 gactcatggc ggtggcaaaa ccgacgccca gcgagcgaat gtcagtagga aaactttccg
    16681 caggcaaaat gcccaccaga ttactgactg ccgaaatggt ggtgctgaag agaacaaaaa
    16741 gcagcagcgt taatgaactg ccggaaggca aacaggccat cactaccagc gttgccgcca
    16801 gcagcaaaaa acttcccagc aaaaatttgc gatgtgccag caggtgcgtc agaactaatc
    16861 ccagcagcgc gcccacaatt aacaacgcat taagcatcag gctggcagtc agcgcatctt
    16921 ccagaccaat agtctgggcg atagttggca gccaggtata aatcacaaac catgggatta
    16981 cgaggcagac aaagaagacg ctgttaaacg ccgtgcgccg ccagtaacgc gaagagaaca
    17041 aggttttgat gtgtttatgg gtcgccgtta ccacttcatc gcccagtaaa acatggggac
    17101 caaaatagcg atgcacgata gcgtgagctt ctgcaaaacg cccctggcgc agtagccagc
    17161 gtggtgattc tggcgttccc cagcgtaata acgtaatcaa caacgcgggc agagctgccg
    17221 atgccagtag ccagcgccag gcctccgggt tttcggaaat aaagtgatgt ccggcaatac
    17281 ttgccagcac atagcctacg gtccacacca cgctgaatgc gcccagcaaa ataccgcgat
    17341 ggcggcgcgg ggaaaattca gccagcaagg tgtgacctac tgaataatcg cctcccagac
    17401 caatgccaat caaaatgcgc agtccaataa gatgctctgg cgtggtggca aaaaattgta
    17461 aaaacgaagc aagcgtaatc agcaaaaagc tgaaggtgaa gattttttgc cgaccaatat
    17521 ggtcggagat ccacccaaga accaggctgc caaggaacaa accaaggagc gccgagccgc
    17581 cgatcattcc cgccataaac ggcgtcagtt gcatggcggg cgtaagctga ataatggcgt
    17641 aaccaatgac gccgagaaca taaccgtcgg tcaggtgtgc gccgaaagtg agcgcagcaa
    17701 tgcggcagtg aaaacggtta agcggtaaat catccattcg caccggtgaa gtgttcatct
    17761 ttaccccttc attgtccggt aataagaacg gcttctcgcc tgagaagccg aaaaggcaac
    17821 gccccgtcat cgcggaacgc tgcctgacac attatttttc aatcggatag atagtacccg
    17881 tattcataat gccattagga tcgaactgct ttttcagccc ttccagcaac gcccacgcgc
    17941 tgccgtgttc cagtttgctc cagtgaacgc gatgtttacc gataccgtgg tggtgcacca
    18001 tcgaaccgcc gaggcgaatg gtttcttcac agatgatctt gttgagcgga ttgtggtact
    18061 tgtcgatttc ctcttccggc ttacagtcaa cgacgttgta atcgtagacg aagtacatgt
    18121 tggtgccgtt ctgatagcta tgagaggaat gaccgcccag catggtgatg tcgtcggcgt
    18181 gcgggaactc agtacgaata cggttaataa cgctttcgta gatttcgtgg atgcagctcc
    18241 agcagccgga cacttcagtg gtaaagccca tgttgccggt tttgaggatc tgcacacgtt
    18301 cggcagccac tttatccggt ccccagttca ggttgttaaa ccaggtttcg atcagcttgc
    18361 tgtccacgcg ctggcattgc gggtagcggg caacgatttc cgcaatccct tcgcccgtcg
    18421 ccttcgcaat gcgagggtta ccttcagcca taaagatcag cacgcatttt ccgtctgcaa
    18481 aatgggtgaa gtgttgggtg ccatcttcag cgtcatacaa acgagcgatc gacggacgat
    18541 acccttccac catgacttca cgcaggatgt tgaagtcggt tttcatgtct tccaggatat
    18601 agccgtagaa gaggttgttt tccggggtga atttaaagat tttcactgtt acttcagtga
    18661 tatagcaaag tgcaccttcg ttaccgatga tgatgtggcg aatgtccggg ccagccgcgc
    18721 ggcgtggcac gtttttaatg cgtgtgacgg tgccatcagc caatactgct tccagaccaa
    18781 cgaccatatc ttcgattgcg ccgtagagtg tggagaactg cccgatactg cgggttgcta
    18841 ccaagccgcc catctgcgcc agcggctttg actgcggaga atgccccgtg gtgtaacctt
    18901 tttcacgcaa cgcattttcc agcacctcca gcggaacccc acattgcgcc gtcgcctgca
    18961 tattctcaat atcaatatta atgatttgat tcatggcgga gccgtcaagc accaccgagt
    19021 tttccacaac ggtttccagc ccaccttcag tggcggaagc gccggtacgc ggcacaccgt
    19081 taattttgtg cgcattcata aaattcagca cacgggatac ttgctctgtg gaaccgagtt
    19141 ttacgaccgc tgccggaatc ggcaaagtat aaatgccatg aatatccgga aatttacgaa
    19201 aacggtcaat actgttcttc tttaatactg tttcatcggt aattacgcga tctgcaccaa
    19261 caatttcctt tagttggtcg acaatcgctg cgcgagataa agacatagta attccttcct
    19321 gataataaaa atggaataat cattaataag agaaaacaga aaccaccgtt atggattagc
    19381 gcactaaata accaccattg accactaata aatgcccatt gacataattt gaagccggac
    19441 ttgcgaggaa tacagctgcg cccattaaat cctgagtatc gccccaacgg tttgccggaa
    19501 tatgatcaag aacgcgctga ttggtttctg gattactgcg tgtcgccagc gtaatatctg
    19561 ttgcataata gccaggagca ataccattta cctgaatatt atattgacct agttcatcac
    19621 aataagcttt ggtgaatccg gcaagagcat gtttagtggc agaatatgca ggtgaccatt
    19681 gtccacctaa gtaagagaac aatgaacaga tattaataat tttgccgctt ttctgcggga
    19741 tcataatttt tgcagcttca tagcttaact cgaatgcggc ggtcaggttc acatcaatca
    19801 tcggatccca gtcggcacga ccgaagtcca gcaccttatt cagcttacaa ataccggcat
    19861 tgtttaccag aatatcaact gtaccgaaac gctcacagca agcagcgata atcttctgcg
    19921 gcgcgccttc tgcggtgata tccacctgca tgaagtccac ctcaacaccc tgtttttcaa
    19981 tcatttcctt tgtttcgccg ttatctttca atcatttcct ttgtttcgcc gttatctttg
    20041 acgaaactag gaataaagat atttgcgcca gctttggcca acgccatggc aaatgcctgg
    20101 cctaaaccgc tattcccacc ggtaacaatt gcggttttac ctttcagtga gaaaaaatcc
    20161 attgagaacg cattgagaga ttcgattgac atagttagct ccaatttccg taaggcaaaa
    20221 aaaagaaagg taacttcccc cgcgagaggg aagttacctt tctcattatc tctggtaact
    20281 ggagataatt tttagcataa cggcataact gctaatgtga tcgtaatcac agtgtgatat
    20341 tcgtattgtc aaatagtttt aaaaagcaga agcgtgatta ctctcacaca acacctctgc
    20401 tttccatgtt atttcttatt gcaattacta gagacctgag aaaagtaatc cacctgaaag
    20461 ggcggcttac ttttctcttt tttttgtcta ttacaaatgg gaatacgata tgcaacacaa
    20521 ctcatatcgc cgttggataa ccctcgcgat aattagtttt agcggcggcg ttagttttga
    20581 cctggcttat ttacgttata tttttcaaat tcccatggcg aaatttatgg gattcagcaa
    20641 taccgagata ggtttaataa tgagtacctt tggtattgcg gctattattc tttatgcccc
    20701 cagcggcgtt attgccgata aattttcaca ccgcaaaatg attacttccg cgatgatcat
    20761 taccggatta ctgggtctgt taatggcaac gtatccaccg ctgtgggtaa tgctctgtat
    20821 tcaggtcgcc tttgcgataa cgacgatttt aatgctgtgg tcggtgtcga ttaaagccgc
    20881 ctcgttgctg ggcgatcata gtgagcaagg gaaaattatg ggctggatgg aagggctgcg
    20941 cggcgtcggt gtaatgtcgc tggcggtgtt taccatgtgg gtcttttctc gctttgcacc
    21001 ggatgacagc gccagcctga aaacagtcat tatcatctac agtgtggttt acatcttgtt
    21061 ggggattctg tgctggtttt ttgttagcga taacaacaac ctgcgcagtg ccaataacga
    21121 agaaaaacag tcattccagc ttagcgacat ccttgccgtt ttgcgtatca gcaccacctg
    21181 gtattgcagc atggtgattt ttggtgtctt cacaatctac gccattctga gttactccac
    21241 caactatctg accgaaatgt acggcatgtc gctggtggca gcgagctaca tggggattgt
    21301 gatcaacaaa atcttccgcg cgctgtgcgg cccacttgga ggcattatca ccacctacag
    21361 taaagtgaaa tcccctaccc gcgtgattca aatcctttcc atcatcggtt tgctggcgtt
    21421 gacagccctg ctcgtcacga actctaaccc ccaatcggtc gcgatgggga ttggcctgat
    21481 tttactgctg ggattcacct gttacgcctc acgcgggctg tactgggcct gccccggcga
    21541 agcgagaaca ccgtcttaca ttatgggcac cacggtaggt atttgttcgg tgattggatt
    21601 cctgccggat gtcttcgttt acccgattat cggccactgg caagacaccc tgcccgctgc
    21661 agaagcctac cgcaatatgt ggctgatggg catggcggcg ctcggcatgg tgattgtctt
    21721 tacctttttg ctgttccaaa aaattcgtac tgctgatagc gcccccgcaa tagctagcag
    21781 caagtaagcc aggaaaatta caggagaaaa atcatgtcga aaaaatacat catagggatt
    21841 gatggcggaa gtcagagcac aaaagtggtg atgtacgatc tggaaggtaa tgtggtttgc
    21901 gaaggtaaag gcttattaca gccgatgcac acgcctgatg ccgatactgc agaacatcct
    21961 gacgacgatt tatgggcatc attatgtttt gcaggtcacg atttgatgag ccagtttgcc
    22021 gggaataaag aagatattgt tggcatcggc ctgggctcca tccgttgctg ccgtgcgtta
    22081 ttgaaagccg acggcacgcc agctgcgccg ttgattagct ggcaggatgc acgcgttaca
    22141 cgcccttacg aacatacgaa tcctgacgtg gcgtatgtaa cctctttttc gggttatctg
    22201 acgcatcgct taaccggcga atttaaagat aatatcgcca actattttgg tcagtggccg
    22261 gtggattata agacctgggc atggagcgaa gatgccgcag tcatggagaa gtttaatatc
    22321 ccccgtcata tgctgtttga tgtgcaaatg ccaggcactg ttcttgggca tatcacacct
    22381 caagccgcgc tggcgacgca tttcccggca ggactgccgg ttgtttgtac caccagtgat
    22441 aaaccggtag aagctctggg agccggatta ctggatgatg aaactgcggt aatttctctt
    22501 ggcacttaca tcgcattgat gatgaacggc aaagccttgc cgaaagatcc ggtggcgtac
    22561 tggccgatta tgtcttctat tccgcaaaca ttgctgtatg aaggttacgg tattcgcaaa
    22621 ggcatgtgga cggtgagctg gctgcgcgac atgttaggcg agtcgttaat tcaggatgcc
    22681 agggcgcagg atctttcacc ggaagattta ctcaacaaga aagcctctag tgtgccacct
    22741 ggctgtaatg ggctgatgac ggtgctggac tggctgacca atccgtggga accgtacaaa
    22801 cgcgggatta tgatcggctt tgattccagc atggattacg catggatata tcgttcgata
    22861 ttggaaagcg tggcgctgac gctgaagaac aattacgaca atatgtgtaa tgaaatgaat
    22921 cactttgcga aacatgtaat cattactggc ggcggttcga acaggatctg tttatgcaga
    22981 tttttgccga cgtgttcaac cttccggcac gccgtaacgc cattaacggt tgtgcaagcc
    23041 tgggggcagc gattaataca ggggtaggtc tggggctata cccggattac gcaacggctg
    23101 tcgataaaat ggttcgcgtg aaagatatct ttataccgat tgagagcaat gccaaacgct
    23161 acgacgcgat gaataaaggc attttcaaag acctaaccaa acatactgat gtgatcctga
    23221 aaaaatcgta tgaagtgatg catggggaat tggggaattg gggaatgcgg attcgatcca
    23281 gagctggtcg aatgcgtgaa taaggtaggc cgggcggaaa actgcccggc ctgaacatac
    23341 ctgaatggtt atccccgctg gcgcggggaa cataagttcg acaacttaaa aaatgataac
    23401 aagtccggtt tatccccgct gatgcaggga acataataaa aactttaact agttcggttt
    23461 atccctgttt ttacaaggaa
//
LOCUS       DAETQN010000062        23236 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.062, whole genome shotgun sequence.
ACCESSION   DAETQN010000062 DAETQN010000000
VERSION     DAETQN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.062"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(38..238)
                     /gene="glgS"
                     /locus_tag="K0W68_002426"
     CDS             complement(38..238)
                     /gene="glgS"
                     /locus_tag="K0W68_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBI3073960.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            507..1136
                     /locus_tag="K0W68_002427"
     CDS             507..1136
                     /locus_tag="K0W68_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HBI3073961.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1163..2824
                     /locus_tag="K0W68_002428"
     CDS             1163..2824
                     /locus_tag="K0W68_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBI3073962.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3063..3122
                     /locus_tag="K0W68_002429"
     CDS             3063..3122
                     /locus_tag="K0W68_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBI3073963.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3439..3498
                     /gene="ibsE"
                     /locus_tag="K0W68_002430"
     CDS             3439..3498
                     /gene="ibsE"
                     /locus_tag="K0W68_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HBI3073964.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="K0W68_002431"
     CDS             complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="K0W68_002431"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBI3073965.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="K0W68_002432"
     CDS             complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="K0W68_002432"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBI3073966.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="K0W68_002433"
     CDS             complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="K0W68_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBI3073967.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9505..10125
                     /locus_tag="K0W68_002434"
     CDS             9505..10125
                     /locus_tag="K0W68_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBI3073968.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10189..11427
                     /gene="cca"
                     /locus_tag="K0W68_002435"
     CDS             10189..11427
                     /gene="cca"
                     /locus_tag="K0W68_002435"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBI3073969.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="K0W68_002436"
     CDS             complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="K0W68_002436"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBI3073970.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12502..12870)
                     /gene="folB"
                     /locus_tag="K0W68_002437"
     CDS             complement(12502..12870)
                     /gene="folB"
                     /locus_tag="K0W68_002437"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBI3073971.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12975..13592
                     /gene="plsY"
                     /locus_tag="K0W68_002438"
     CDS             12975..13592
                     /gene="plsY"
                     /locus_tag="K0W68_002438"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBI3073972.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWSVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="K0W68_002439"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="K0W68_002439"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            14744..15655
                     /gene="ttdA"
                     /locus_tag="K0W68_002440"
     CDS             14744..15655
                     /gene="ttdA"
                     /locus_tag="K0W68_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HBI3073973.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15652..16257
                     /gene="ttdB"
                     /locus_tag="K0W68_002441"
     CDS             15652..16257
                     /gene="ttdB"
                     /locus_tag="K0W68_002441"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBI3073974.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16306..17769
                     /gene="ttdT"
                     /locus_tag="K0W68_002442"
     CDS             16306..17769
                     /gene="ttdT"
                     /locus_tag="K0W68_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBI3073975.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="K0W68_002443"
     CDS             complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="K0W68_002443"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBI3073976.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19062..19277
                     /gene="rpsU"
                     /locus_tag="K0W68_002444"
     CDS             19062..19277
                     /gene="rpsU"
                     /locus_tag="K0W68_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBI3073977.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19388..21133
                     /gene="dnaG"
                     /locus_tag="K0W68_002445"
     CDS             19388..21133
                     /gene="dnaG"
                     /locus_tag="K0W68_002445"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBI3073978.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21328..23169
                     /gene="rpoD"
                     /locus_tag="K0W68_002446"
     CDS             21328..23169
                     /gene="rpoD"
                     /locus_tag="K0W68_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBI3073979.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5652 a         6331 c         5907 g         5346 t
ORIGIN      
        1 aatacaaaca ctaaaacggg ccatcaggcc cgttatatca gtgctctaac tccagctctc
       61 ttgcctgcat catctgttgg caataccagg cataacgtgc gcaaaaccag gttctatgtt
      121 cttcatccat gttaccagta acggcaagaa tatcgaccgg gcgaccttct gcgtgcattc
      181 tggcaaaact acgcgccagg aaatcgaaat tatttaaaga attaagacta tgatccataa
      241 gcacatcctc cggttatcgc atacctttta aggccatatg ctttttctga tttcagtatt
      301 ccccaatcag tgtagtgcct ttaaacataa cgtgcgtaaa tatattgtcc ccgatcacac
      361 tttttagtga aaaataaaaa actatattaa aaaactttct cttccagaac ttctctaaca
      421 tgtgttatta ttaacaaaga taaatgtaag caaggaaact atcccaaagt aaatattgcg
      481 tcactaaatg gacattggag tgacatatga ttttattcgc cgactataat accccttatc
      541 ttttcgctat ttcctttgta ctgttgatcg gccttttaga aattttagcg cttatctgcg
      601 gtcacatgct ctccggtgct cttgatgcac atcttgacca ttataattcc ataaccacag
      661 gtcatattag ccaggcactt cattatctca atatcggaag attgccagct ctcgtcgttc
      721 tctgcttgct ggctggtttt tttggtctta ttggcatcct gttgcagcat gcctgcatca
      781 tggtctggca atcaccactg tcgaacctgt tcgtggttcc tgtcagttta ctgtttacga
      841 ttattgcggt gcattatacc ggtaaaatag ttgcgccctg gattccacgc gaccatagtt
      901 cagctattac agaagaagaa tatattggta gcatggcatt aattaccggt catcaagcca
      961 cttcaggtaa tccctgcgaa ggaaaactca ccgatcaatt tggccaaata cattatttac
     1021 tactggagcc tgaagaagga aaaattttca ctaaaggagt caaagtgtta attatttgcc
     1081 gactttctgc aacgcgatat ttggcggaaa acaatccctg gccacaaata ttgtaattta
     1141 ttaatcaaaa ggaaatttaa taatggatga tattgttaat tctgtgccct cctggatgtt
     1201 taccgcgatt attgccgtat gcattctgtt tattattgga attattttcg ccaggctcta
     1261 tcgtcgcgct tcggcagagc aagcttttgt tcgtactggt ttaggtgggc aaaaagtggt
     1321 gatgagcggt ggcgcaatcg tgatgccgat ctttcatgaa ataatcccca tcaatatgaa
     1381 tactctgaag ctggaagtca gccgctcaac cattgatagc ctgattacga aagatcgtat
     1441 gcgtgtcgat gtagtagtcg ctttctttgt acgggtaaaa ccgtctgtag aaggcatcgc
     1501 cactgcggcg cagacgttgg ggcaacgcac gctatcacca gaagacttac gtatgttggt
     1561 tgaagataaa tttgtcgatg ccctccgtgc aacagctgca caaatgacca tgcatgagtt
     1621 acaggatacc cgcgagaact tcgtgcaggg agtacaaaat actgtcgcag aagacctatc
     1681 gaaaaacggt cttgagctgg aaagcgtttc acttaccaac tttaaccaga cctcgaaaga
     1741 acatttcaat ccgaacaatg cctttgacgc cgaaggttta accaaactga ctcaggagac
     1801 ggagcgccgt cgtcgcgaac gtaacgaagt tgaacaggat gtagaagttg cggtgcgtga
     1861 gaaaaaccgt gatgcacttt cgcgcaagtt ggagattgaa caacaagaag cgtttatgac
     1921 gcttgagcag gagcagcagg ttaaaacccg tactgccgaa cagaatgcac gtattgcggc
     1981 ttttgaagct gaacgtcgtc gtgaagcaga gcagacgcga attctggctg aacgacaaat
     2041 tcaggaaacg gagatcgagc gcgagcaggc cgtccgctca agaaaggttg aggctgaacg
     2101 tgaagttcgt attaaagaga tcgaacagca gcaggtcacc gaaatcgcca accagacgaa
     2161 atcgatcgct attgccgcca aatcggaaca acagtcccag gcagaagcgc gtgctaacct
     2221 tgcactcgca gaagcagtaa gtgcgcaaca aaacgtagaa accactcgcc agaccgcaga
     2281 agccgatcgt gctaaacaag ttgccctaat cgctgccgcg caggatgcag aaaccaaagc
     2341 ggttgaactg accgtgcggg cgaaagcaga aaaagaagcc gcagagatgc aggcggcggc
     2401 tatcgttgag ttagccgaag ctacacgtaa aaagggtctg gcggaagcag aagcacaacg
     2461 tgcgctgaac gatgctatca acgtactttc tgatgaacaa accagcctta aattcaaact
     2521 ggccttgttg caggcgctac ctgcggtaat agagaaatcc gttgagccga tgaagtccat
     2581 tgacggtatc aagattattc aggtcgatgg tctgaatcgt ggcagcgctg cgggtgatgc
     2641 aaacacgggt aatgtggggg gcggaaacct agcggaacaa gcattatcag ccgctctctc
     2701 ttaccgcaca caggcaccgc tgattgactc cttgctcaat gaaattggcg tttcaggcgg
     2761 ctcactggcg gcattgactt cacccttaac ctcaacaact cccgtcgccg aaaacgtaga
     2821 ataaattttc agctatgaac gtccctggca caaaaagccg gggatttttt atatctgcgt
     2881 tccgctaaaa actgcaaatg atcaggccgt tgcaacgttt tgcgtgaccg ctcggggaag
     2941 gcaaaactgc ctctgggaaa gcgttgcgcg aggtccggcg ctcatcaaca atcggggagt
     3001 cagcaaaggg ctgaaacggg aaagcccctc ccgaggaagg ggccttacag aaggaaaggg
     3061 ttatgatgaa gctcgtcatc atactgattg tgttgttact cgtaagtttc gcagcttatt
     3121 aacagccaat cagagggggg agaaatcctc cctcaccctt gttcctttac tttaggttga
     3181 aaaaaaatca gcgtcaagaa ggtttcatta acacatatcc ctggcccaaa aagccgggga
     3241 ttttttatat ctgcgttccg ctaaaaggtg caaatgctca ggacgttgca gcgttttgcg
     3301 tgatcgctcg gggaaggcaa aactgcttca gggaaagcgt tgcgcggggt ccggcgctca
     3361 tcaacaatcg gggagtcagc aaggggctga aacgggaaag cccctcccga ggaaggggcc
     3421 ttatagaagg aaagggttat gatgaagttc gtcatcatac tggttgtgtt gttactgtta
     3481 agtttcccga cttactaaca actgatcaga gggggaagaa atcctccctt acccttgttc
     3541 ctttacttta ggttgaaaaa acaacagcgt caataggcct gcctgctaca aagcgagatc
     3601 tgtgaaccgc tttccggtta gcctttttta tcctgttgga tcttcttgat gatgttagtg
     3661 gtcgagcaac cgtcttcgaa gttgagcacc agcacttcgc cgccgttggc ccagacttct
     3721 ttactcccgg cgatctcttc tggtttatag tcgccgccct tcaccagtaa atctggcagg
     3781 atcccggcga tcaagcgctg cggcgtgtct tcttcaaacg acaccaccca gtcgaccgct
     3841 tccagtgcgc ccagcacaat catacgctgt tcgagcgggt taacggggcg ggaatcccct
     3901 ttcagccgtt tggtggaggc atcgctgttc acagcgacaa tcagacggtc accctgtttg
     3961 cgagcatttg ccagatagga gacgtgcccg gcgtgcagga tgtcaaagac accgttggtc
     4021 attaccacct tttcgccgcg tttacgcgct gccgctacgg ccagcttcag ttcctcttcg
     4081 gtcatcacgc caaagccggt atctgcgcgc ccacgcactg cattttccag ctcgatcggc
     4141 gaaaccgtgg aggttcccag tttgccgacc accacgccag ccgccgcatt ggcaaagaag
     4201 caggcttctt ccagcgaatt acccgctgcc agcgttgccg ccaggacgcc aatcaccgtg
     4261 tcgcccgcac cggtaacgtc atacacttcc tgcgcctggg ttggcatatg cagcggcgct
     4321 ttacccggtt gcagcagcga cataccctgt tcggaacggg tcactaacag agccgagagt
     4381 tcgtaatcgg caatcagttt catgccgcgc tcaacaatct cttcttcggt cttacattta
     4441 ccgacaacag cttcaaattc cgagagattc ggcgttaaca gcgtagcgcc gcggtagcgc
     4501 tcaaaatcgg taccttttgg atcaatcagc accggaacgc ccgctttacg cgccagttgg
     4561 atcatctgct gtacgcttgc cagcgcacct ttggcgtagt cagaaagcac cagcgcgcca
     4621 atcgaactca gcgcctgatt aatccgctcg tgcagcggct gcggatcaac gccttcgaaa
     4681 ccttcttcga aatccagacg gatcagctgt tggttgcggg aaagtacccg taatttggtg
     4741 atggtcggat gcgtcggtac agaaacgaag tcgcatttga cgttgacgtc ggccagagat
     4801 ttactcagcg cgcgcgctgc atcgtcaatg cccgtcaacc cgaccaggcg tgcattagca
     4861 ccgagagaag cgatattcat cgccacgtta gccgcgccgc ccggacgttc ttcgatggta
     4921 ttcactttaa ccacgggcac cggcgcttcc ggcgagatac ggctggaggg accgtaccag
     4981 taacgatcca gcattacatc accaaccacc atcactcctg cacgttcaaa ctctggcagc
     5041 gttactttca ttcctgtctc ctgagagatt caaaatttgc gcgcgataat accatacttc
     5101 attcttccac cagccacttc tgccagcttg cccgcaccag atcacgctct gcggtgaagc
     5161 aatcccccga cacatggccc ggcaattcct gtaatgccag atgatgaagt tcatcgcgca
     5221 gcgtcgtgta agcacgggtc agcgccatcg cttcctgctc ttccataatg tcgttttgcg
     5281 ccagcagctc cagaatacgc acgttgtctg accagcgcgt taacttcggt ttttcatgag
     5341 cgtagcgcaa caccagatat tgggtaataa attcgatatc ggtaattccg ccttcatcag
     5401 ctttgatatc aaagcgatcg cgatgtttat tgccgagatg agcgcgcatt ttctcgcgca
     5461 tttcccgcac ttccgtttgc agagttctac cttcacgcgg cagcgtcata atctcgcgac
     5521 gcactgcgtc aaagtgcgcg gtgagctgcg gatcgccgta cactacccgc gcacgcacca
     5581 gcgcctgatg ttcccacgtc caggcctcgt ttttctgata atcggcaaat gcttctgtgg
     5641 aggtaaccag cattcccgcc gccccggacg ggcgcagccg cgcatccact tcgtacaaaa
     5701 tgccggaaga ggttcgcgtg ctgaacaggt gcataatgcg ttgcgccaga cgaagataaa
     5761 actgtcgtcc gtcgatttcc cgctcgccgt cagtcatcgc atccattggg caatcatgga
     5821 ggaagatcag gtcaagatcg gaactgtagc ctaactccca gccgcccagc ttgccgtagc
     5881 cgaccactgc aaaaccacgc ccttcgcgat cgttgaggtg attcggctta ccgtagcggg
     5941 caaccatttg aacccacgcc tgctgaacga cggcatctat catggcttcc gccagccagg
     6001 ttaagtgatc gctcactttc atcaccggta gcgtaccagc gatatccgct gcggcgatgc
     6061 gtaacagctg cgcctgtttg aactgacgca gcgcctcaag ctgttgctct tcgtcatctt
     6121 ccggcacgcg cagcaaatac tggcgcaact catcgcggta ggcatcggtc gccgtcggct
     6181 ggtaaagagt gtttggatcg agcaattcat ccagcaataa tggataacgc gccagctggc
     6241 tggcaatcat cggcgatgcg gcacacagag aaatcaaatg gttaagcgcc gcagggaatt
     6301 cactgagcaa ttctaaatag gtggtgcggg taacaatccc caccagcaag gcggtaatgc
     6361 gcgacagcgt gacagcagcg tcttcacgcg cacagacatc acttagcaga tgcggcatca
     6421 gatggtcgag cacctgacgt cctcgcggcc cgatggtgcg cttatccagc tctttgcgga
     6481 aatcggaaat cagcgttagc acctgtttgc gatcatcctc gctaagatgc gccagcactg
     6541 gcgtagtgtc atcttcctgc aacgcatcct gccacagctc acgccactgt tccgacagcg
     6601 actcttcctg agtttcactt tcgtcgtcgc caatcaattc attaaacacc cggcgcacat
     6661 tgttcatatg tgcggtcagc gccccggtca gttgcggcca gtcagcaaag tccatcgccc
     6721 acgccagccg cgcacggtta agctcatcag aaggaagcgt ctgggtttgt tcgtcgttaa
     6781 tgctttgcag caggttttcc agacgccgca ggaacagata cgccactcgc aattgttcag
     6841 catcgttttc agaaagcaga tgcagcgcag caatggcgct gagcgttggc agtaaagagc
     6901 acgattgcag cgacggttcg cgcccgccgc gaatgagctg gaatacctgg acgataaatt
     6961 caatttcgcg aataccaccc gcgccaagtt tgatgttgtc ggtcaaaccg cgccgacgca
     7021 cttcacgggc aatcattcct ttcatgttgc gcagcgactg aatcacgctg aaatcgatgt
     7081 aacgacggaa aacaaacggg cgtagcatcg cacgcaactc gttagcatag acgccttcgc
     7141 tatcgcccat aatccgcgct ttgaccatcg cgtaacgctc ccagtcgcgc ccctgctcct
     7201 ggtaataatc ctccaacgcg gcaaagctca gcaccagcgg gccactttcg ccaaacggac
     7261 gcagtcgcat atccacgcga tagacgaagc catcctgcgt tggttgatcc agcactttaa
     7321 tcagccgctg ccccatgcgg gtaaaaaact gcgcgttatc cagttcccgg cgtccgccct
     7381 gcgtacaacc gtgttccggc caggcaaaaa tcagatcgat atcagaggag aaattcagtt
     7441 ccccgccgcc cagcttaccc atgcctaaga tcagcagcgg ttgcgcttcg ccctgcgcat
     7501 tgcacggcgt tccccactcg cggcagcagg cgtcatacag ccagtcacgc gccgcaacaa
     7561 tcagcgtctc cgccagataa ctgagctgct gcaatatgct ctcttcagta accagtgcca
     7621 gcgtttgcgc ccatgcgatg cgcaccataa tgcgccgccg gaatagccgc agctcgcgca
     7681 ttaacccggc ttcgtcactc acattactga gcgcctcctg caaccatgca gcgtaatgct
     7741 gccattcatc ggcctgcggc ggttggcttt ccagttccgt caaccactct cgatgcgcaa
     7801 ttatgctgtc ctgcacaaaa tcactgaaag taagtactga cttcgcctgt gcacttagtg
     7861 gttcctcggc taaaggctct ggcagccgct caacaacggt ctgcctgtac tgctgtaacg
     7921 gtgaagagag cggcttcata agcgatttta tccttgcctg attaacgttt tccgctgtgc
     7981 aaccagaacg gttcctgatt gtttgcctca ttacggaaat gctcaatttc aatgcgttgc
     8041 ccggtcgcaa tagcgtgacg cagcccctgc cagttctcca gccaggcatg cgcgacgaca
     8101 ggatcatagt aacccgccag caacagtatt gagtcaatgt cacgcgtcag gcgtggcaac
     8161 tggtcacggt agagatcgcc taacggctgg cagaaaacgc ttttcagttc agcggcatgg
     8221 tgggaaagat ggatatcggc aaagcgtttg aaggagtcgc tgattttgcc ctgcgctttg
     8281 gcatctaaaa atggctgcca tgctttgctt accaaccatt cggtcaacgc cagcttcgcc
     8341 attgccgttt cggtagagta gaccgccgtc acggcagaaa ccgcagaagc aatggtcgct
     8401 tcgcattggg tcagcaaatc acgtaagtga gtgctcgctt tacgcggcac aataccaccg
     8461 aacagcatca gcgtatgacg gaccaggcta atggctgcca gcacctgttc tttcgccgca
     8521 tcgttaccgc gtacccacag ttcttcatga tactgccatt gcgctaacgc cagctcgagc
     8581 gccgcttcca gcccctgttc tacatcggct tttgccgcaa catgcaaaat ggtggtcggt
     8641 ttgatttcac gcgccggatt gccctgcgcc agatgatagc cacgcgccgc tttgctcagg
     8701 ctgccctggc gtaatccggt ttgcgatacc agttggttcg ccagtttcag caccgcgcgc
     8761 gtgtcgccgc taagcagttc cagttccagc tcacagatag gttcagcaaa ttcccccgct
     8821 ttcacctccc cctgatcgag agcgatttca attcggctgc catcgaccgc caccagccat
     8881 ttttcgcgat aaaaatcggt gctgaacagc ggttgtacgc gggaagcgag atcggcaggc
     8941 aattcaccgt tcggccagac ttccgaggga aactgcgcca ggtcgagcgt cggttcgctc
     9001 aacgccacgt tatattccgg gcgctggtgt aagccgcctg tcactcttcc tgcaactttc
     9061 atggtcatct catagcgacc gttttcgcca cgaatacgta agcccatatc gtgcctacgc
     9121 agccagttat ccggcgtttc gtagtaaata ttcagcaact gcacggggtc atggtgctcg
     9181 ccgcccagcg tattgagatg gtcacgcaac gcctcaacgg cactgtgatt aacaataaac
     9241 tttaattcga tttcctgagc catggccttt acttatcggt tatgtcacat aagtgacgat
     9301 gaacggcgaa cttaatgcga tcttttttgt cagtagatag tattttgcgc caaattgcca
     9361 tgcaacgggc aatttgacgg gcgtaaaagt ttgaagcagt ggcaagacct gacacagagg
     9421 attccgattg atgtcgaatg ctttgcgtag cgacactgat gccactacta tcgttccact
     9481 ttccatgaca ataacgacag cctgatgcca aaattacgcc tgatcggatt aactttactc
     9541 gcacttagcg cgactgccgt ctcacacgct gaagaaacgc gctatgtttc tgacgaactg
     9601 aatacctggg tccgtagcgg tccgggagat cattatcgcc tcgtgggcac ggttaacgcc
     9661 ggcgaggaag tgaccttatt acaaactgac gccaacacca attatgccca ggtgaaagac
     9721 agctctggcc gtaccgcctg gatcccgttg aaacaactta gcactgagcc aagcctgcgc
     9781 tcccgtgtgc cagatctgga aaatcaggtc aaaaccctga ccgataaact caccaatatc
     9841 gataacacct ggaatcagcg caccgcagaa atgcagcaaa aagtggcgca gagcgacagc
     9901 gtgatcaacg ggttaaaaga agaaaatcag aaactgaaaa acgagctgat tgtcgcgcag
     9961 aaaaaggtcg atgccgccag cgtacagctg gatgacaaac agcgcaccat catcatgcaa
    10021 tggtttatgt atggcggtgg cgtgctgggg cttggcttgc tgctcggtct ggtactgccg
    10081 cacctgatcc caagccgcaa acgcaaagat cgctggatga actaaatcgc cttcttcgtt
    10141 gcactgtctt attattagga attgtgtatt tgacgaaggg atgatggcgt gaagatttat
    10201 ctggtcggtg gtgctattcg ggatgcattg ttagggctac cggtcaaaga cagagattgg
    10261 gtggtagtcg gcagtacgcc acaggagatg ctcgacgcgg gctaccagca ggtaggccgc
    10321 gattttcctg tgtttctaca tccgcaaacg catgaagagt atgcgctggc acgtaccgaa
    10381 cggaaatccg gttccggtta caccgatttt acttgctatg ccgcaccgga tgtcacgctg
    10441 gaagatgatc ttaagcgtcg cgatctgacc attaatgcgc tggcccagga cgataacggc
    10501 gagattatcg acccgtacaa cggtctgggc gatctgcaaa atcgtctgtt gcgccatgtt
    10561 tcccccgctt ttggcgaaga tccgttacgc gtactgcgcg tggcgcgttt tgctgcgcgt
    10621 tatgcccacc tcggttttcg tattgccgat gaaactctga cgctgatgcg cgagatgacc
    10681 catgcgggtg aactggaaca cctgacgcct gaacgggtat ggaaagagac ggaaagcgcc
    10741 ctcaccaccc gcaatccaca ggtgttcttc caggtactgc gcgattgcgg cgcactgcgc
    10801 gttttattcc cggaaattga cgcactgttt ggcgttccgg cccctgccag gtggcatccg
    10861 gaaatcgaca cgggtattca taccttaatg acgctctcaa tggcggcgat gctgagtccg
    10921 caggtcgatg tccgtttcgc aactttatgc cacgatctcg gtaaaggact gacgccgcca
    10981 gaactttggc cgcgtcatca tggtcacggt ccggcgggtg tgaaattagt ggaacaactc
    11041 tgccagcgtc tgcgcgtgcc aaatgaaatt cgcgatttag ccagactggt ggctgagttt
    11101 cacgatctca tccacacctt cccaatgctg aacccaaaaa ctatcgtcaa attgtttgat
    11161 tccatcgacg cctggcgtaa accgcagcgt gtcgagcaac tggcgctgac cagcgaggct
    11221 gacgtacgcg gcagaaccgg ttttgaatca gcggactacc cgcaaggccg ttggttgcgc
    11281 gaagcctggg aagtggcgca gtcagtgccg acaaaagcgg tcgttgaagc gggatttaaa
    11341 ggtgtggaga ttcgcgagga gctgacccga cggcggattg cggcggtagc cagttggaag
    11401 gaacaacgtt gcccaaagcc tgactgagta taaaactaaa cgttaacacg aaccatcccc
    11461 tcgccccttc ggggagaggg ttagggtgag gggaacagac cagcaccggt gcgaacaatc
    11521 acccccaccc cggccctctc cctacagagc gagggagaaa accaggcctg ctcccgaacc
    11581 ttttctcaat caaaagaaca cgacgtacac cgcagccgcc acaataaagc gataaatggc
    11641 gaacgggata aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg ctatcagcgc
    11701 caccacaaaa gcggtgataa acccaacggc aaacatcgag atatcgccgg ttgtcaggaa
    11761 gccccagctt ttgtagagat cgagcgccgt tgcgcccatc atcatcggta ccgccagcag
    11821 gaacgagaac tcggaagcag cgtaacggct cacgcccatc agcatcccac ctgaaatggt
    11881 ggccccggaa cgggagaaac ccggccacag cgccagacac tggaaacagc caatcataaa
    11941 cgcctgacga taggtcatat catcaagacc cggcgcacgc ggctctttcg gcttcaggca
    12001 ttcggcggca atcagcaaca aaccgccaac gaccagtgca tacatcacat ttatcgggtt
    12061 aaacaatgac ttaatcgtgt cgtggaacaa cagccccagt accaccgccg gaatcatccc
    12121 cagcaaaatg tggatcagcg ttaaacgacc tttgctttct ccttcgtgct gcaacgggcg
    12181 gccaaagtgg atgccaatca ggccaaacag acgccgccag aacatcacta ctaccgccag
    12241 aattgatcct aactggatca caacttcaaa ggttttcgcc gtgtcgccct cgaaccccaa
    12301 caagtgaccg acaataatca tatggcccgt gctggatacc ggcagaaatt ctgtcaatcc
    12361 ttcgaccaca cccaatattg ccgctatcag cagcgagtgc atatcgctca tcaataaacc
    12421 cctaaattat taaaatgtac cgcttgtccg gactactgca gtaagaccag gttataaccg
    12481 tttggtttaa cagctgtaaa attaattatt ttctttcaga ttattgccac gctcaatgat
    12541 tacaccaaca ttcgccgccc gcgccattgc gcctggcttg ctgagtttga tgcgcaccca
    12601 cggcgagttg aagcgtgcta acagcagctc cgccacctct tcagccacgc gttccaccag
    12661 cgcaaaacgc gccccctcga cgtggctgac caccgtttct gcaatgtcag cgtaactgag
    12721 gcaatccgcc acatcatcac ttttcgccgc tttacggtta tcccatgcca tttcgatatc
    12781 gaacactaac ttctgttcga tggtctgttc ccagtcgtaa acaccaatag tggtgattac
    12841 cgaaagttgc tctataaata caatatccat cacgtcctgc ctgctttttg gctaaccgga
    12901 taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgttgaaca cgaattttca
    12961 aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg
    13021 gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg gctgcccgat ccgcgaacca
    13081 gcggctccgg caatccaggc gcaaccaatg tgttacgtat cggtggcaag ggagcagccg
    13141 tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc gcgtatgaat
    13201 taggtgtcag ccccttctgg ttaggcttaa ttgccatcgc cgcctgtctt ggacacatct
    13261 ggtccgtttt cttcggattt aaaggaggaa aaggcgttgc taccgctttt ggtgccatcg
    13321 cacccattgg ctgggatctc accggagtaa tggcgggaac ctggttactg accgtgctat
    13381 tgagcggata ctcgtcgctg ggagcgattg tcagtgcact gattgctccg ttttatgtct
    13441 ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg atcctgctgc
    13501 gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat
    13561 tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat gacctgatag
    13621 ccttcatcgg gcttgcccag ccgttgctgg caccatgccg ccagaaattc cacgcagaca
    13681 cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt ggcgctttgt
    13741 gcatactccg gcaatacttg caccagtttg ccactctcca gaaacggcaa cacatcccac
    13801 tcggaacgca gcataatccc tttgccctcc agcgcccatt gcaacactat ctcgccgcta
    13861 ttggaggaaa gatgcccgct aaccttcacc gattttttct cctgaccgtt ccccaactcc
    13921 catattccgt gggtcatatc gcgctctttg gtcaccagac agtcatgacg acttaattcc
    13981 tgtaatgatt gtggctgcgg atatttttgc agatactcag gtgctgcgca taatattctt
    14041 ttatttttcg ttaacagatg cgcaatataa taatcaggaa tttcgtcatt aatacgaata
    14101 tccagatcaa tattatcctg cactaaatca atttgccgat cgaacaattc aaaatgcacc
    14161 tgtaactcag gataattgcg cataagttcg gtaatggctg gcgcaatatg gctgcgccca
    14221 aaaccaaaac tgcaaccaat acgaatcatc ccctccgggc gcgttttgat ttacgtgacg
    14281 tcatcgacca gtcgctgata ttgggtgagg atttccagcg catgttcgta acaacgttgg
    14341 ccactttcag tcagcgccac gccgcgagcg gagcggttca gcagcgttgt tgccagcgta
    14401 ttttcaagga tttggatccg cttggtgaca aatgcaggcg tctgcccaag cgtcgccgct
    14461 gcggcgctaa aactcccgct gtggacgatt tctaccagta cctgcaagtc tttggctaaa
    14521 ggccagctat tcagcatcgt taattatccg cagttgtgat aagcgcagtg tatttcgcaa
    14581 aacattgcca ttccctgctc tggcgcaata ttcattctta ttttcgcgtt gattcacgaa
    14641 ttgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag tggtagtttg
    14701 tggcggtgaa tttttccaga caaatacaaa aactggagtt gccatgatga gcgaaagtaa
    14761 taagcaacag gcagtgaata agttgacgga gattgtcgct aactttaccg ccatgatttc
    14821 tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc
    14881 gtcgatgggg aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct
    14941 gaatcgtcct gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg
    15001 cttcccactg cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt
    15061 caaagcgcca ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa
    15121 taccggtagc ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcgga
    15181 aatcgaagtt tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat
    15241 gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc
    15301 cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc
    15361 cgccgtactc tcgcgtaaag ccattttgcg cccgattggc agccgccatc ccaatccaaa
    15421 agcggcagaa ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca
    15481 agggctgact ggcaacagtt cagtgatggg cgtacatatc gaatctgccg cccgccatcc
    15541 gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg catcgtcgcg gcacactgct
    15601 ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag
    15661 atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcga cgatgtgatc
    15721 tacctgaccg gtacgctggt gacctgccgt gacgtttgtc accgccgctt gatcgaactg
    15781 aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg ccccatcgtg
    15841 cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc cgacaaccag tatgcgtatg
    15901 gaaagttttg aacgtgaatt tatcgagcag accggcgtga aactggtggt tggcaaaggc
    15961 ggaatggggc cgctgaccga agaaggctgc cagaaattca aggcgctaca tgtgattttc
    16021 ccggcaggct gcgcggtgct ggcggcaacc caggtggaag agattgaaga agtgcactgg
    16081 acagagctcg gaatgccgga gtcgctgtgg gtctgtcggg tcaaagagtt cggcccgctg
    16141 attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc
    16201 gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat caaataaccc
    16261 tcccggagag gctcgccccc tctccttttt cgcaggcata acacgatgaa accttccact
    16321 gaatggtggc gataccttgc gccgctggcg gtcatcgcca ttattgctct aattccggtt
    16381 cccgcagggc tggagagtca tacctggctc tactttgccg tttttactgg cgtgatcgtt
    16441 ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat atccatcatc
    16501 gccatactct ctccctggct gctgttcagc ccggagcagc tcgctcagcc aggctttaaa
    16561 ttcactgcaa aatccctctc gtgggccgtt tccggttttt ctaattcggt tatctggctg
    16621 attttcgccg cctttatgtt tggcacaggc tatgaaaaaa ccgggcttgg acgccgcatt
    16681 gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt ttctcggcta tgcggtgatg
    16741 ttctccgagc tgatcctcgc acctgtaaca ccgtccaact cggcgcgtgg tgcggggatt
    16801 atctatccca tcatccgtaa cctgccaccg ctctatcaat cacaaccaaa cgacagcagt
    16861 tcgcgcagca ttggctcgta catcatgtgg atggggattg ttgccgactg cgtgaccagc
    16921 gccattttct tgacggcgat ggcaccaaac ttgctgttaa ttggactgat gaaaagcgca
    16981 tctcacgcca cgctgagttg gggcgactgg ttcctcggga tgttgccgct cagtattttg
    17041 ctggtgctgc tggttccctg gctggcttat gtgctgtacc cgccggtact gaagtctggc
    17101 gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt
    17161 gaaaaacgga tgctggggct gatagtaggc gcgctggtgc tgtggatttt cggcggtgat
    17221 tatatcgatg ccgcgatggt cggttacagc gtggtggcac tgatgctgct tctgcgcatt
    17281 atcagttggg acgacattgt cagtaataaa gcggcgtgga acgttttctt ctggctggcc
    17341 tcgcttatca ccctcgctac cggactcaac aacaccggtt ttattagctg gtttggcaaa
    17401 ctgttagcag gcagcttaag cggttattcg ccaacgatgg tgatggtggc gttgattgtg
    17461 gtgttttatc tgctgcgcta ctttttcgcc agcgccacgg cgtatacctc cgccctcgca
    17521 ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc cgctgccggt gttctgcctg
    17581 atggttggcg cggcaattgg tctggggagc attcttacac catacgccac cggacccagt
    17641 ccgatttact acggtagtgg ttatctgcca acggcggatt actggcgact gggggcgatt
    17701 tttgggctga tattcctcgt gttgctggtg attaccggct tactgtggat gcccgtggtg
    17761 ttgctttaag tgaggacggt gccggaaatt gctctccggc acccaatagt tttacgcagc
    17821 cggtaactcc gccagcggcc agcgcggacg cacgctaacg ccgagatccg ccgtcgcgcc
    17881 tgctttaaac cgcaccattc cggcataggc gatcatcgcg ccgttatcag tacaaaactc
    17941 cggacgcgcg tagaacactt cgccgcggcg ttttttcatc atttcagcca gcttcgcccg
    18001 taacgtgcgg ttagcactca cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc
    18061 caacgctcgc ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc
    18121 gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt
    18181 tttcagacca gaaaagctga aatccagccc cggacggtcg gtcatcggac gcgggaaaac
    18241 aaagcgcccg gcagtaccct gagccgccat tttcgacagt aacggtccgc caggataatc
    18301 cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg gcggcatcat cgatagactc
    18361 gccgagcagc tcgtactgac caatgccagt cacgctgatt aactgcgtat gaccgccgga
    18421 caccagcagc gcgacaaacg gaaattccgg cgggttatct tccagcatcg gcgctaacag
    18481 atgcccttcc atatggtgta ccgggattgc cggaacgttc caggcaaacg ccagagaacg
    18541 ccccacggtt gcgccaacca gcagtgcgcc gactaatcca gggcctgcgg tataggccac
    18601 agcatcaatg tcttttgccg ttaagccaga ctcctttagc gccgcctgga tcaacggtac
    18661 ggttttgcgc acgtgatcgc gggaggccag ttcaggcacg acgccgccat agtcagcgtg
    18721 caatttcacc tgactataca attggttggc taacaaacct ttttcatcgt cgtaaatggc
    18781 gatgccggtt tcatcgcagg aagtttcaat acccagtaca cgcatgactt gttttacctc
    18841 gctttattac cgcgcagtgt aggaccaatg cgggttgatg taaaactttg ttcgcccctg
    18901 gagaaagcct cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa ggccgcggtg
    18961 ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt
    19021 gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta attaaagtac
    19081 gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag
    19141 gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc
    19201 gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc
    19261 gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga gttgtagttg
    19321 taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc
    19381 ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg cacgcactga
    19441 catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc
    19501 gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg agaaacagtt
    19561 ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga tgaactacga
    19621 caagctcgag ttcgttgaaa cggtcgaaga gctggcagca atgcacaatc ttgaagtgcc
    19681 atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa cgctttatca
    19741 gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg ttgccacgtc
    19801 tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc gctttgcgat
    19861 tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca atccagaaaa
    19921 tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac gcagttacga
    19981 tcgtttccgc gagcgggtga tgttccccat tcgcgataaa cgcggtcggg tgattggttt
    20041 tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg aaacagacat
    20101 tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata acgctgaacc
    20161 caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc aatacggcat
    20221 taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac aactgttgtt
    20281 ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc
    20341 atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc tacgctttat
    20401 gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta aagaagcgtt
    20461 tgaagcgcgg atggagcagg cgatgccgct ttccgcattt ctgtttaaca gcctgatgcc
    20521 gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg cactgccatt
    20581 gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat taggcaacaa
    20641 attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg cagagagcgg
    20701 cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta tagggttgct
    20761 ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg atgaaaataa
    20821 gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc agccaggtct
    20881 gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca cccttgaaaa
    20941 actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct tcaccgactc
    21001 actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa tcgctcgtga
    21061 gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa accaggagct
    21121 ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc cccgacagcc
    21181 gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt ggcggtaaac
    21241 aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc aacaccaacc
    21301 tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca gctgaaactt
    21361 cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa tgaccatctg
    21421 ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat caacgacatg
    21481 ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc tgaaaacacc
    21541 gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga atctgaaatc
    21601 gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt tgaactgttg
    21661 acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa ccaggttcaa
    21721 tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta cgatcgtgtt
    21781 gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc gaacgcagaa
    21841 gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga agatctggac
    21901 gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga tgacaacagc
    21961 atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta cgttgtaacg
    22021 cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga gatcctgaaa
    22081 ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta cctggtcaac
    22141 agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat gaagctctgc
    22201 gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg caacgaaacc
    22261 agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga aaaactgcac
    22321 gatgtctctg aagaagtgca tcgcgccctg cagaaactgc agcagattga agaagaaacc
    22381 ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg tgaagcgaaa
    22441 gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat ttctatcgcc
    22501 aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg caacatcggt
    22561 ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc cacctacgca
    22621 acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg caccatccgt
    22681 attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg ccagatgctg
    22741 caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct gatgccggaa
    22801 gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga aacgccgatc
    22861 ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct cgagctgccg
    22921 ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct ggctggcctg
    22981 accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta tcgatatgaa caccgaccac
    23041 acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg tcagatcgaa
    23101 gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg tagcttcctg
    23161 gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag tccctctgca
    23221 caaacgccac cttttc
//
LOCUS       DAETQN010000063        23156 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.063, whole genome shotgun sequence.
ACCESSION   DAETQN010000063 DAETQN010000000
VERSION     DAETQN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23156
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.063"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            230..1663
                     /gene="cscA"
                     /locus_tag="K0W68_002447"
     CDS             230..1663
                     /gene="cscA"
                     /locus_tag="K0W68_002447"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBI3073980.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1671..2729)
                     /locus_tag="K0W68_002448"
     CDS             complement(1671..2729)
                     /locus_tag="K0W68_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI3073981.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2897..3115
                     /locus_tag="K0W68_002449"
                     /pseudo
     CDS             2897..3115
                     /locus_tag="K0W68_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3133..4461
                     /gene="dsdA"
                     /locus_tag="K0W68_002450"
     CDS             3133..4461
                     /gene="dsdA"
                     /locus_tag="K0W68_002450"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBI3073982.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="K0W68_002451"
     CDS             complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="K0W68_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBI3073983.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6107..7270)
                     /gene="emrK"
                     /locus_tag="K0W68_002452"
     CDS             complement(6107..7270)
                     /gene="emrK"
                     /locus_tag="K0W68_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBI3073984.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7686..8300
                     /gene="evgA"
                     /locus_tag="K0W68_002453"
     CDS             7686..8300
                     /gene="evgA"
                     /locus_tag="K0W68_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBI3073985.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8305..11898
                     /gene="evgS"
                     /locus_tag="K0W68_002454"
     CDS             8305..11898
                     /gene="evgS"
                     /locus_tag="K0W68_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBI3073986.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11954..13099)
                     /gene="yfdE"
                     /locus_tag="K0W68_002455"
     CDS             complement(11954..13099)
                     /gene="yfdE"
                     /locus_tag="K0W68_002455"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBI3073987.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="K0W68_002456"
     CDS             complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="K0W68_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBI3073988.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="K0W68_002457"
     CDS             complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="K0W68_002457"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBI3073989.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15935..17185)
                     /gene="frc"
                     /locus_tag="K0W68_002458"
     CDS             complement(15935..17185)
                     /gene="frc"
                     /locus_tag="K0W68_002458"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBI3073990.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17698..18321)
                     /locus_tag="K0W68_002459"
     CDS             complement(17698..18321)
                     /locus_tag="K0W68_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBI3073991.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18618..18893
                     /gene="ypdI"
                     /locus_tag="K0W68_002460"
     CDS             18618..18893
                     /gene="ypdI"
                     /locus_tag="K0W68_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HBI3073992.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18970..19212)
                     /locus_tag="K0W68_002461"
     CDS             complement(18970..19212)
                     /locus_tag="K0W68_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBI3073993.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19565..20485
                     /gene="lpxP"
                     /locus_tag="K0W68_002462"
     CDS             19565..20485
                     /gene="lpxP"
                     /locus_tag="K0W68_002462"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBI3073994.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="K0W68_002463"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="K0W68_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBI3073995.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="K0W68_002464"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="K0W68_002464"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBI3073996.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22592..>23156
                     /gene="ypdA"
                     /locus_tag="K0W68_002465"
     CDS             22592..>23156
                     /gene="ypdA"
                     /locus_tag="K0W68_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HBI3073997.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIV"
BASE COUNT         6486 a         5420 c         5044 g         6206 t
ORIGIN      
        1 actttggctg acattttttt cccttaaatt catctgagtt acgcatagtg ataaacctct
       61 ttctcgcaaa atcgtcatgg atttactaaa acatgcatat tcgatcacaa aacgtcatag
      121 ttaacgttaa catttgtgat attcatcgca tttatgaaag taagggacta tatttttata
      181 aaagttaacg ttaacaattc accaaatttg cttaaccagg atgattaaaa tgacgcaatc
      241 tcgattgcat gcggcgcaaa acgcactagc aaaacttcat gagcaccggg gtaacacttt
      301 ctatccccat tttcacctcg cgccacctgc cggatggatg aacgatccaa acggcctgat
      361 ctggtttaac gatcgttatc acgcgtttta tcaacatcac ccgatgagcg aacactgggg
      421 accaatgcac tggggacatg ccaccagcga cgatatgatc cactggcagc atgagcctat
      481 tgcgctagcg ccaggagacg ataatgacaa agacgggtgt ttttcaggta gtgctgtcga
      541 tgacaatggt gtcctctcac ttatctacac cggacacgtc tggctcgatt gtgcaggtaa
      601 tgacgatgca attcgcgaag tacaatgtct ggctaccagt cgggatggta ttcatttcga
      661 gaaacagggt gtgatcctca ctccaccaga aggaatcatg cacttccgcg atcctaaagt
      721 gtggcgtgaa gccgacacat ggtggatggt agtcggggcg aaagatccag gcaacacggg
      781 gcagatcctg ctttatcgcg gcagttcatt gcgtgaatgg accttcgatc gcgtactggc
      841 ccacgctgat gcgggtgaaa gctatatgtg ggaatgtccg gactttttca gccttggcga
      901 tcagcattat ctgatgtttt ccccgcaggg aatgaatgcc gagggataca gttaccgaaa
      961 tcgctttcaa agtggcgtaa tacccggaat gtggtcgcca ggacgacttt ttgcacaatc
     1021 cgggcatttt actgaacttg ataacgggca tgacttttat gcaccacaaa gctttttagc
     1081 gaaggatggt cggcgtattg ttatcggctg gatggatatg tgggaatcgc caatgccctc
     1141 aaaacgtgaa ggatgggcag gctgcatgac gctggcgcgc gagctatcag agagcaatgg
     1201 caaacttcta caacgcccgg ttcacgaagc tgagtcgtta cgccagcagc atcaatctgt
     1261 ctctccccgc acaatcagca ataaatatgt tttgcaggaa aacgcgcaag cagttgagat
     1321 tcagttgcag tgggcgctga agaacagtga tgccgaacat tacggattac agctcggcac
     1381 tggaatgcgg ctgtatattg ataaccaatc tgagcgactt gttttgtggc ggtattaccc
     1441 acacgagaat ttagacggct accgtagtat tcccctcccg cagcgtgaca cgctcgccct
     1501 aaggatattt atcgatacat catccgtgga agtatttatt aacgacgggg aagcggtgat
     1561 gagtagtcga atctatccgc agccagaaga acgggaactg tcgctttatg cctcccacgg
     1621 agtggctgtg ctgcaacatg gagcactctg gctactgggt taacataata tcaggtggaa
     1681 caacggatca acagcggaca agggatccgc gtcactcttc ccccttcacg accttcaata
     1741 atatgcaatg cagcttcccg cccgataatg tcatgtggaa gctgaattgt ggtcagcggc
     1801 ggtaaaaaca gatgcccgac gccaaccaga ttatcaaagc ccattacggc gacatcctgc
     1861 gggatacgta cccccttcgc caggagaacc tgataagcga caaaggctgc acgatcgtta
     1921 ccacatatca gaacatcaaa atctggtttg cccgatttga agtgggcatt gagtaaactt
     1981 gcgagatcgg tgtagtgatc atcacctgtt gccatgtgaa attgtttcac ctcagccaga
     2041 tctcgtccag catcacgcca ggcctgctca aatccctgcc gacgataccc tgttgccaac
     2101 gcactttccg gtagccagaa gcataacggt tgacgatagc ccgccgcgag caaatgctgt
     2161 gttgattcat attgtgcagt gtaatcatca gggatataac tgggtaacgc tgggtcatcc
     2221 gccacacagt tcgccaatac aatattttca ccatacagag actcaggcag cgtgatatgt
     2281 cgcagcccca ttgtagtata gataatgcca tccggacggt gggcaagcag ctgacgtgcc
     2341 gcgcgggcag cgtcatcttc agaaaaaata ttgattaaaa aactattcca gccgaactcg
     2401 ctggcggttt gctcaatggc aagcagaata tcaacagaga aaggagtggt agccgtgtcc
     2461 tgcgccagca cggcgagagt cgacggctta cgtccttgag cgcgcatctt acgggcggaa
     2521 agatcaggaa cataattcag ggtctggatt gcctgcaata cgcggtcacg cgttgcagga
     2581 cgcacagatt ctgcattatg catcacccgg gagactgtca tcatcgacac tcccgccagg
     2641 cgtgcgacat cctttaatga agccataccc aagccgtttg ccgtaaaacg ggcactgtag
     2701 cagaaacaga cgtcactggc gagatccaac gccctatcac ctgacacagc aatacaataa
     2761 aaaataacaa taattcccgg acaattgtcc ccaattccgc ctctgttctc gccaacgagt
     2821 aaccatatag gcaagtaagt aaaacatgcc aactggcatc gccaacctgg agaagaaaat
     2881 ataaatatgg atcattgtgc tgccgctgta tcccgacatc agcccggaaa ttattgcgat
     2941 tgctatcggt tcaggtgcaa ttggctgcac gatcgttacg gactcgcttt tctggctagt
     3001 gaagcaatat tgcggcgcta cgctcaatga aacatttaaa tactatacga cagcgacatt
     3061 tatcgcttca gtcgtcgctc tggcgggcac attcctgctg tcatttatca tctaagcgca
     3121 aagagacgta ctatggaaaa cgctaaaatg aactcgctca tcgcccagta tccgttggta
     3181 aaggatctgg ttgctcttaa agaaaccacc tggtttaatc ctggcacgac ctcattggct
     3241 gaaggtttac cttatgttgg cctgaccgaa caggatgttc aggacgccca tgcgcgctta
     3301 tcccgttttg caccctatct ggcaaaagca tttgctgaaa ctgctgccac tggggggatt
     3361 attgaatcag aactggttgc cattccagct atgcaaaaac ggctggaaaa agaatatcag
     3421 caaccgatca gcgggcaact gttactgaaa aaagatagcc atttgcccat ttccggctcc
     3481 ataaaagcac gcggcgggat ttatgaagtc ctggcacacg cagaaaaact ggctctggaa
     3541 gcggggttgc tgacgcttga tgatgactac agcaaactgc tttctccgga gtttaaacag
     3601 ttctttagcc aatacagcat tgctgtgggc tcaaccggaa atctggggtt atcaatcggc
     3661 attatgagcg cccgcattgg ctttaaggtg acagttcata tgtctgctga tgcccgggca
     3721 tggaaaaaag cgaaactgcg cagccatggc gttacggtcg tggaatatga gcaagattat
     3781 ggtgttgccg tcgaggaagg acgtaaagca gcgcagtctg acccgaactg tttctttatt
     3841 gatgacgaaa attcccgcac gttgttcctt gggtattccg tcgctggcca gcgtcttaaa
     3901 gcgcaatttg cccagcaagg ccgtatcgtc gatgctgata accctctgtt tgtctatctg
     3961 ccgtgtggtg ttggcggtgg tcctggtggc gtcgcattcg ggcttaaact ggcgtttggc
     4021 gatcatgttc actgcttttt tgccgaacca acgcactccc cttgtatgtt gttaggcgtc
     4081 catacaggat tacacgatca gatttctgtt caggatattg gtatcgacaa ccttaccgca
     4141 gcggatggcc ttgcagttgg tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg
     4201 ctggatggct tctataccct tagcgatcaa accatgtatg acatgcttgg ctggctggcg
     4261 caggaagaag gtattcgtct tgaaccttcg gcactggcgg gtatggccgg acctcagcgc
     4321 gtatgtgcat cagtaagtta ccaacagatg cacggtttca gcgcagaaca actgcgtaat
     4381 accactcatc tggtgtgggc gacgggaggt ggaatggtgc cggaagaaga gatgaatcaa
     4441 tatctggcaa aaggccgtta ataacgtttc aacgcagcat cgcaatcctt tccctgggtg
     4501 agcgatgctg ccgatggcgc agacttaaga tccccggtct tacccgctat aaccccctcc
     4561 ttttatcatc acccaacgcc tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc
     4621 gtaagtaaga taaaagcaat agccgccata cggaaaatct catttgcaga aatagaaagt
     4681 gactgctggg ttatttcatt attaatttca ttaagaactc ctgaaagcga accataatat
     4741 ttatccataa tttgtgacga tgaattaaac acggggttaa attgatcgat ggttgctgtc
     4801 aactgactat ggtgtaacga ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca
     4861 actgatcctg acaaggtacg aaaaaaatta ctcatactcg aggcattggc aaatttatta
     4921 tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat
     4981 ccctgaaaaa actgcggcaa aatgatgcct gtaaaatcaa tcgttggcat aaatgtcaca
     5041 gaacgccagt aatagcaaac cgcatacatc aaaaaactaa atgtcactaa caaccgcatg
     5101 tctattttgt tgccataacg tcctatcaaa ggtgaaatta atagtggcat gatgccgatg
     5161 ggcgcataag caagtccggc ccatatcgca ttatacccca tcgtttcctg gagtaactgc
     5221 ggcataagga cgatcgctcc agagtaaaat aaatacgcgc atgtgatact cacaatacca
     5281 atggtgaagt tacgggactt aaacaaactg agatcaagaa tcgggttctc tgaggtcgac
     5341 tcccaaatga ctaaagagat cagagaaata actgatacta ctgttaatat tattattgta
     5401 ctcgagttga accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca
     5461 ccgagcacta acagggtcag tcctggtaga ttcattttga ccggtgaagt ctcagtttct
     5521 cttcctttaa gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt
     5581 aaaaatatcc aaccccagct aaagttatca caaatataac cgcccaatat cggcccacat
     5641 atcggagcga taatcacggt cattgaccat aatgccagag caaatgttct tttttctggc
     5701 ggataattcc ttaataataa actctgtgac agtggaatta acggccccgc cattaacccc
     5761 tgaacgactc taaaaaatat cagcacatca agattggtcg ataggctaca cattaatgaa
     5821 gacagcgaaa aaaaggttac tgaaagtaaa aataatctta attcgcctat tctttgtgcc
     5881 aacctgccag taacagggat cgcaatggca tttgctacac caaacgaggt gataacccag
     5941 gtgccttcgt ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa
     6001 atagtggaat ccaacatttg cataaatgtc gctaatgaca atgcaatagt gacgcaccat
     6061 aacgtcccac cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat
     6121 tatgcgaaat aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct
     6181 tactggtata agccggcatg gaggtcacgg ttgaagccag ctcaggcatc tcagcaatgt
     6241 cttcgtcctt cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca
     6301 tgagttcttt tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt
     6361 tccctgtcgc attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc
     6421 ctgtcacccg accatgaaac acaacatttt caccataaag atcgctgata atattgactg
     6481 attgaccaat ccgtacatcc gtgagttgtg tttctttaaa gttggcatta acccacattt
     6541 gacgtgccgg tacgacagcc attaacgatt gtccgggact cactgtttcg ccgacctgaa
     6601 cacttcgctg ggcaatatag ccagtaaccg gactccttat atccgtacgt ttaagcgcca
     6661 accaggcttc tttggttgca tccgccgctt caactacttg tggctgacgg tttaatggtg
     6721 tgttcattac taaagcttta ttcgctttat aagcctggat agcggcattc agtgccgctt
     6781 tgctacttat taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct
     6841 tcgctaaagg cacacgacgg ttataatctt ctaaggattg ttgatactga atacgtgctg
     6901 aagcgacttc ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa
     6961 tatttgccag attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg
     7021 aaactaaaat gtcaccctgt cgaacgtagt tcgtatcttt atgattaact acagtgacac
     7081 tacctgagac ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac
     7141 taatcatgtc ttctaattcc attgaccaat aggcataggc acctgaaaac gcaataaata
     7201 aaactatcac caataaagaa aagtattttt ttctgttaga ctgtttttta tttgaattaa
     7261 tctgttccac tattatctct catttcgcat agatgaaatt tatgaattga atgaaaagag
     7321 tattcattct tcatatcaaa cacagtgcat gtgttgcaca ttatcccagg aacgttggag
     7381 gcctaattta aatgaggtgc aaaaaaaaca acagaggact aaaccgtggc ttttgcaata
     7441 caattcttac gcctgtagga ttagtgagaa gacttatagt gctaacttga aactataaat
     7501 catcggtata atccctgatt ttattgttga catttcattt atgccgacta tttatatggt
     7561 atacttgtcg aattatctta aaggaagctc agattttctt atttttattg agaaaatgag
     7621 atgacgcctt atgtctgtat tactacaggg agaagggaga tgcttcattg caaagggaat
     7681 aatctatgaa cgcaataatt attgatgacc atcctcttgc tatcgcagca attcgtaatt
     7741 tattgatcaa aaacgatatt gaaatcttag cggagttaac tgaaggcgga agcgccgttc
     7801 agcgggtgga aacacttaag cctgatatcg tcatcattga tgtcgatatc cccggagtta
     7861 acggtatcct ggtgttagaa acgctgagga agcgccaata tagcggaatt attattatcg
     7921 tctccgctaa aaatgaccat ttttacggga aacattgtgc tgatgctggc gctaatggtt
     7981 tcgtgagtaa aaaagaaggc atgaacaata tcattgcggc tattgaagct gcaaaaaatg
     8041 gctactgcta tttccccttc tctctcaacc ggtttgttgg aagtttaacg tccgaccagc
     8101 aaaaactcga ctccttatcg aaacaagaaa ttagtgtcat gcggtatatt cttgatggca
     8161 aggataataa tgacattgcg gaaaaaatgt tcatcagcaa caaaactgtc agcacttata
     8221 aaagtcgcct gatggaaaaa ttagaatgta aatcactgat ggatctttac acattcgcac
     8281 aacgtaacaa aatcggctaa ccacatgaag tttttaccct atatttttct tctctgttgt
     8341 ggtctttggt cgaccataag tttcgcagac gaagattaca tcgaatatcg tggcatcagt
     8401 agtaacaacc gtgtcatact tgatccagta cgtctgagca acaaggaatt acgttggtta
     8461 gcgagcaaaa aaaatcttgt gattgcagta cataagtccc aaacggctac gttgttgcat
     8521 accgattcgc agcaacgagt tcgtggtatt aatgctgatt atttaaatct tttaaaaaga
     8581 acgttaaata tcaaattaac actccgggaa tacgccgatc atcaaaaagc aatggacgcg
     8641 ctggaagaag gtgaagtcga tatagtgtta tcacatttag tagcttcgcc gcctcttaat
     8701 gatgacattg ctgcaaccaa accactgata attacctttc cggcgctggt aaccaccctt
     8761 cacgattcaa tgcgaccgct tacctcatca aaaccagtaa atattgctcg agtagcaaat
     8821 tatccccctg acgaggtaat tcatcaatca tttccaaaag caacaattat ctcttttaca
     8881 aatttatatc aggcattagc atccgtctca gccggacaga atgattactt tattggtagt
     8941 aacatcatta ccagcagtat gatttcccgc tatttcactc actccttaaa tgtagtgaaa
     9001 tattataact caccgcgtca atataatttt ttcttgacca gaaaagaatc tgtcgttctt
     9061 aatgaagtac tcaatcgatt tgttgatgct ttgacaaatg aagttcgcta tgaagtatca
     9121 caaaattggc ttgatacagg aaacctggcc tttctgaaca aaccattaga actcactgaa
     9181 catgaaaaac aatggattaa gcagcatccc gatttaaagg tgctggaaaa tccttactcc
     9241 cctccctatt ctatgacgga tgaaaatggc tcggttcggg gcgttatggg ggatattctt
     9301 aatattatta ctttgcaaac aggtttaaat ttttctccga tcaccgtttc acacaatatc
     9361 catgctggaa cacagctgaa tcccggcgga tgggatatat tacccggtgc tatttatagt
     9421 gaagatagag aaaataatgt tttatttgct gaagccttca taacaacgcc ttacgttttt
     9481 gtcatgcaaa aagcgcctga cagtgaacaa acattaaaaa aaggaatgaa agttgccatt
     9541 ccatattatt atgagcttca ttcgcaatta aaagagatgt atccggaggt agagtggata
     9601 aaagtcgata acgccagcgc tgcatttcac aaggtcaagg aaggcgaact tgatgctctg
     9661 gtcgcgacac agttaaattc acgttacatg atcgaccatt actatcctaa tgaactttat
     9721 cattttctta ttcccggcgt tccgaatgca tcgctttcgt tcgcttttcc tcgcggagaa
     9781 ccggaactta aggatattat taataaagca ctgaatgcaa tccccccaag cgaagttctg
     9841 cgcctgaccg aaaaatggat taaaatgccc aatgtgacca ttgacacatg ggacctctat
     9901 agcgagcaat tttatattgt tacgacatta tccgttttat tagttggcag tagcctttta
     9961 tggggatttt acctgttacg ctcagttcgt cgtcgtaaag tcattcaggg tgatttagaa
    10021 aaccaaatat cattccgaaa agcactctcg gattccttac cgaatccaac ttatgttgta
    10081 aactggcaag gtaatgtcat tagtcataat agtgcttttg aacattattt cactgcggat
    10141 tactacaaaa atgcaatgtt accattagaa aacagtgact ccccctttaa agatgttttt
    10201 tctaatgcgc atgaagtcac agcagaaacg aaagaaaatc gaacaatata cacacaggtt
    10261 tttgaaattg ataatggcat cgagaaaaga tgcattaatc actggcatac attatgcaat
    10321 ctgccagcca gcgatcatgc tgtttatatt tgtggttggc aagatattac agagacgcgt
    10381 gatttaattc atgcactcga agtagagaga aataaagcga tcaatgcaac tgtcgcaaaa
    10441 agtcagtttc tggcaacaat gagtcacgaa ataagaacac caataagctc cattatgggc
    10501 ttcctggaac ttctgtcggg ttctggtctt agcaaggagc aacgggtgga ggcgatttca
    10561 cttgcctact ccaccggaca atcactcctc ggcttaattg gtgaaatcct tgatgtcgac
    10621 aaaattgaat cgggtaacta tcaacttcaa ccacaatggg tcgatatccc tactttagtc
    10681 cagaacactt gtcactcttt cggtgcgatt gctgcaagca aatcgatcgc attaatttgc
    10741 agcagtacat ttcctgatca ttacctggtt aagatcgacc ctcaggcgtt taagcaggtc
    10801 ttatcaaatt tactgagcaa tgctctcaaa tttaccaccg agggggcagt aaaaattacg
    10861 acctccctgg ttcacattga tgacaaccac gctgttatca aaatgacgat tatggattct
    10921 ggaagtggat tatcgcagga agaacaacaa caactcttta aacgctacag ccaaacaagt
    10981 gcaggtcgcc agcaaacagg atctggctta ggcttaatga tctgcaaaga attaattaaa
    11041 aatatgcagg gcgatttgtc attagaaagt catccaggca taggaacaac atttacgatc
    11101 acaatcccgg tagaaattag ccagcaagtg gcgactgtcg aggcaaaagc agaacacccc
    11161 atcacactac ctgaaaagtt gagcatatta atcgcggatg atcatccgac caacaggcta
    11221 ttactcaaac gccagctaaa tctattagga tatgatgttg atgaagccac tgatggtgtg
    11281 caagcgctac acaaagtcag tatgcaacat tatgatctgc ttattactga cgttaatatg
    11341 ccgaatatgg atggttttga gttgactcgc aaactccgtg agcaaaattc ttccttaccc
    11401 atctgggggc ttacagccaa cgcacaggct aacgaacgtg aaaaagggtt aagttgcggc
    11461 atgaacttat gtttgttcaa accgttgacc ctggatgtac tgaaaacaca tttaagtcag
    11521 ttacaccagg ttgcgcatat tgcacctcag tatcgccacc ttgatatcga agccctgaaa
    11581 aataatacgg cgaacgatct acaactgatg caggagattc tcatgacttt ccagcatgaa
    11641 acgcataaag atctacccgc tgcgtttcaa gcactagaag ctggcgataa cagaactttc
    11701 catcagtgta ttcatcgcat ccacggtgcg gctaacatcc tgaatttgca aaagttgatt
    11761 aatattagcc atcagttaga aataacacct gtttcagatg acagtaagcc tgaaattctt
    11821 cagttgctga actctgtaaa agaacacatt gcagagctgg accaggagat tgctgttttc
    11881 tgtcagaaaa atgactaaat agcggctccc acaatgttct aatgtgggag ctatttacca
    11941 gcacatctta catttatgat gagaactcct ggcgaatttg ttccccatgc tggtcgagcg
    12001 ttgccgctcc cggcataaca tgcgggtccg cgcagccgct gatttttatc ggattacccg
    12061 gcatcattat tcctccggct tcaatcaaca tatttctcgc ctgagtttgt ggcaaattaa
    12121 tggcctcagc cacacttaat aacggcgcga cgggcacacc aacttcatgt attttggcta
    12181 accaaacttc agctgcctgc gtttttaacg ttcgctcaat atattgttta agaatagcct
    12241 ggttttgtac gcgtaaaata ttgctgctaa atcggggatc attaaccagt tccgtaagct
    12301 ccagtgcctg gcataacgca gaaaaaagct tgtcattacc acaacaaatc gtaatcggct
    12361 tatcctgagt atcgaaaaca tcaaaaggtg ccatgtaggg atggcgattt cccaggcgtt
    12421 gtggtgactt ccctgtcgca atatatgcca tcagaccatg ctccagaaaa ctcagcgtgg
    12481 catcaaacat cgctatatcg acatgcgccc ctctctggct cttttcgcgg ccataaagtg
    12541 cactcactat tccgctgaat aaatagacac cgccgcatag atccgcaaga gaggtaccaa
    12601 cacgcactgg cggagcatca gggtaccctg tttccatcat tatcccactc attgcctgaa
    12661 tgatggtatc gtaggcagga gcatctttta gcggaccggt atgtccgaaa cctgacgatg
    12721 aagcatatat gaggcgcggg ttgatttctt gtaacctttc ccatgaaaac cccagttttt
    12781 ccattgtacc tgggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa
    12841 atatactttt atcgtgatca ttctttaaat caagaaccac actctctttg ccatgattaa
    12901 taaaactgta atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac
    12961 catgacctgg tggctcaact ttaattaccc ttgcgcccat attacaaaga agttgagttc
    13021 cgaaaggtcc attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt
    13081 tgctttcatt atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt
    13141 cggcatttat gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc
    13201 cacaagggag ccgtgacgac aaagcccagt acgctcacag ccaatgacgc tgtaccagta
    13261 cgggtgtaga cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg
    13321 cctgccagta ccatcatctg caaatgttcg ctgttcaaat gacatgccat accaacgaga
    13381 agcagcgcca gtggcatcag aatcaacttc aggaaggtgt tataagcaat ttcggcactg
    13441 aactcgaatt tatgtgcagc cagagtcaac cccgcagcga ataccgctac ccctgagtta
    13501 gccttcgcaa tcagattaaa ggtcgggtcc catgctgccg gaatttttac cccaaccagc
    13561 accaggatcg ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat
    13621 gcgctcagat tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga
    13681 ccaataggaa tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt
    13741 gatacggaat caccataaat aggatcgaga actgcaaacc ccaggaatcc aatggtaggt
    13801 gaacccgcaa ttaatgcaca tacagcggct tctgcatggg tacgtttaaa aaatttgtag
    13861 caaccaaacc aagagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga
    13921 cgagtgtccg caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca
    13981 ggaagagcgt agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt
    14041 tctcgtctgc cgctaaaata acccaacagc atgattacga taataggcaa taaatcgcca
    14101 ataaaaaatg ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag
    14161 ttggcaagag gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg
    14221 gtaatatggc cactttcagt tcctgcagca gggtcgatga ccacattaat aatggtcggt
    14281 ttgcgcgact gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta
    14341 tagccaacgc cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc
    14401 gttggtgatg gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca
    14461 ttattaaaaa taacgatcgt caccggcagg ttatatcgac aaatcgtttc aatttccatc
    14521 ccactgaaac caaaagcact atcaccttca atggcgacaa ccggagaacc agaagtcacg
    14581 ctagcaccga tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga
    14641 cgacgacgtg gtttatacat atcaataata tttcgtgcat tatccagggt atttgcacct
    14701 tcattaacta aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca
    14761 ttaaagtaat ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt
    14821 tgctgcttgt ggatatttaa aatatcgcgc cataccagtg gagtcgtaaa tgtgttttgt
    14881 ttcagttctg ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca
    14941 atggggcggt tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct
    15001 gccgcccatc cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg
    15061 acaacgtcag catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta
    15121 tcttcaagga tccctttcgc catagacatt ggcaggaatg gaatctgagc actttcaata
    15181 aattcacgaa gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat
    15241 ggccgttcag cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat
    15301 aatgctggcg acggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc
    15361 gtcgcggcca ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca
    15421 gagacccgga tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat
    15481 gctgctttgg catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc
    15541 tgtaggtcga cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt
    15601 accgttgcgt tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg
    15661 cagatccccg gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc
    15721 tcatgacgaa aaccaatata acgaatgcct tccgcctggg catggcgtgc catatccgtc
    15781 acaggaatac ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg
    15841 atgatatgca taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt
    15901 tttataaatt gggagccaga agcccccgtt aatatcagat ggcgtggttt tgcttcattg
    15961 cagcaatttc atcgtcgcta taacccagct cctgcaatac agcagcggta tgttcaccta
    16021 atagcggcgc agctttaata tccggcgtaa aggcagagaa tttcattgga caaccaacgg
    16081 tcagatattt tccacgcaac ggttgttcca cttcaacaac actgccactt tggcgcaaag
    16141 agggatcaag tgaaatttct ttcatactta aaaccggtgc acaaggaata tcaaactgag
    16201 tcaaataggc caccgcttca tgtttatcaa tagtgacagt gtatttttcg atttcagcaa
    16261 aaatatcgaa aatatgtggc tgtcgggcat gggctgtact gtatgccgaa tcggtaatcc
    16321 attctggttt gccgatggct ttacaggtgt tttcccagtt ttgctcctga atagtgaaat
    16381 aaatataggc gttaggatcg gtttcccagc ctttacattt caggatccag ccaggctgac
    16441 cgccaccacc tgcattacca ccgcggggaa ctgcatcacc aaatgtacca ttcggatact
    16501 gcgggtattc ttccagataa cccaatttat cgagacgctg ctggtcacgt aatttcacgc
    16561 ggcaaaggtt caatacggca tcctgcattg acatggtgac tcgttgccca cgccccgttt
    16621 tttcgcgatg cagcaaagca gcaagtaaac cgatcagcaa atgcattccg gtgttgctgt
    16681 cacccaacgc tgcagcgctt accagcggcg gaccatccca aaaacccgta gtggatgccg
    16741 cgccacccgc tgcctgagca acgttttcat aggcttttac attcacataa ggcgaacact
    16801 catcaaaccc tttgatcgaa ccaaaaatca gacgtggatt gatttcttga atatgctccc
    16861 aggtgaagcc catgtgatca atggcccctg gatgaaagtt ctcgactaag atatcagctt
    16921 cgcggatcag cttttccatt acctctttgc cttccgctgt tttggtattt aactcaatag
    16981 aacgtttgtt actgttaagc atggtgaagt aaagcgcatc gatatcagga atatctcgca
    17041 gttggtgacg cgttacgtca ccaacgccgg gacgttcaat tttaataacg tcagcgccaa
    17101 accaggccag catttgagta caagatgggc cagattgcac accggtgaaa tcgagaactt
    17161 taattccttg aagtggagtt gacatagcaa tacctattag ttaattaaat atgtgttaat
    17221 ttagcgttta tttaaggcat aacgccctct ataaataatc tgcaaaatat cttcgtagcg
    17281 gcactcgata tttttaaatt cctgtatcaa gtactgaaaa ttatttcatc gccacttcca
    17341 attcctttct gcatggtaag tagtgaggac gaaaagaaga atatgataaa tatttattcg
    17401 tggtaatcag agagagtcgc gacatttgtg atgttattct tacaactgta agaagcgccg
    17461 gtgatttaca agtaagagta tgattacgct ttttaaagcg taccataaag cataacataa
    17521 taaatgattt gtgatgacac tggattattg cggtttactt tgaggaaaat aaaatgcgtg
    17581 tttatagaac cgcgagcgcc aaataatttt atgaccacaa cctgtcgtat tgtggtcata
    17641 aaaaattgtc ggctggaggg agttaaatcc tttacctcaa tatactgcct gtcagcatta
    17701 gttcacaaac agtgcttcgc tatcgacgat gattccatct tccgcacctt taagcgccag
    17761 gtttgcctcg tagtattgct ttttatcaag cagtttctgc gcatcagcaa gtgcattacg
    17821 agtctgttta agcggcatca gatattggtt ttccattacg ccaacaccgg ccaggcgaag
    17881 ctcttccatt gcgccctttt tatccccttt cgccattttt tcattggcaa tttttatcgc
    17941 ggcttctttt tcgggtgttg ctacataact ctcagaaatt ccgacagatg cattaatgac
    18001 aatatattgg tcatcattga gattggtctt tttacctggc ttagcgaact ttgcccattc
    18061 ggtactgtca tctgacagca aagcggaagc ttcattggtc agttcttttg ccttttccgg
    18121 atcgccatga aacagagcca ggcgcgctac ctgcacgtca cgcatcgcat ataaaccttg
    18181 ttcagagata cgctctgctg ctgccgtttt ctgggtttgc tgagtttgct gttgtgccgc
    18241 taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt
    18301 agccataatt aaacgtttca tacttacctc attctttaac agttttagaa aacgccgcca
    18361 tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg aaagggttat
    18421 cttacaattg tagttattat cgcaaagcgg catttagtct tacagttgta ggaatatgct
    18481 tcctgtcgca gagaactatt cttatatacc acaaagagat gtcatttatt atattgatgt
    18541 aaatattgat taattatatg taaatacagc cataacatcg tatggttctc agtgaaccat
    18601 tttcaggata actttctatg aaagtaaact taatactttt cagcttattt ttattggtct
    18661 ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct
    18721 ataaagagac tgaaaaaaca tcagcgatga caaccacaca ctctacaaaa cttcagccat
    18781 cacaggcgat tttgtttaag atgagagaag atgcgccacc attaaacctc acagaagaaa
    18841 tcacgcctac ttatccgaca aaggcgaatt atcttattca tcctgtgcga tagcgtctgt
    18901 cacatggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat
    18961 cctgacccac tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg
    19021 ggcaagaaac ataaatatcc ccgtcaccag ttcattaccg ccagttaacc acatggtgaa
    19081 gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac
    19141 gttcagtacc tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca
    19201 taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta
    19261 atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcacgt
    19321 taaatgcaac gctattgcgt gcggaaaatt ttttactttg cacgattaat aatcagtagc
    19381 tgaaagcagt cagccagtga taaagtagag ccaccgcatt catgctcctc gtggttatgt
    19441 cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt
    19501 gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt
    19561 aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac
    19621 atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt
    19681 tctcggcacg cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc
    19741 ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat
    19801 tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg
    19861 gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc ctaaacgcgc
    19921 acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg
    19981 cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca
    20041 gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgatcggcag
    20101 aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc
    20161 cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt
    20221 cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt
    20281 aacgatggta agaaaagcgg attacagcgg atatcgtttg tttatcaccc cagagatgga
    20341 aggctacccg acagatgaaa atcaagccgc tgcctatatg aacaagatta tcgagaaaga
    20401 gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt
    20461 gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc
    20521 cataataacc tcatggtttt tatggctttt ttcttaaggt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattaa cgccgtcttg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacggcg
    21301 gcgtttgtac tgttcggtaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag cgtttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atccatggct ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaattacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cgacgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgcgt cagtattctc ggcggcatgt tgtgtgagac
    23041 gttgaccatg attctggtaa ttgtctgggc accaactacc gcgttgggga tcgatatcgt
    23101 ctctaaaatc ggcattccaa tgatcctcgg tagcgtctgt atcggcttta ttgtgc
//
LOCUS       DAETQN010000064        22595 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.064, whole genome shotgun sequence.
ACCESSION   DAETQN010000064 DAETQN010000000
VERSION     DAETQN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22595
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.064"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            238..1344
                     /gene="ompC"
                     /locus_tag="K0W68_002466"
     CDS             238..1344
                     /gene="ompC"
                     /locus_tag="K0W68_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBI3073998.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1456..2511
                     /gene="apbE"
                     /locus_tag="K0W68_002467"
     CDS             1456..2511
                     /gene="apbE"
                     /locus_tag="K0W68_002467"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBI3073999.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2585..3649
                     /gene="ada"
                     /locus_tag="K0W68_002468"
     CDS             2585..3649
                     /gene="ada"
                     /locus_tag="K0W68_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBI3074000.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3649..4299
                     /gene="alkB"
                     /locus_tag="K0W68_002469"
     CDS             3649..4299
                     /gene="alkB"
                     /locus_tag="K0W68_002469"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBI3074001.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4375..6018
                     /gene="yojI"
                     /locus_tag="K0W68_002470"
     CDS             4375..6018
                     /gene="yojI"
                     /locus_tag="K0W68_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBI3074002.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6236..7882
                     /gene="mqo"
                     /locus_tag="K0W68_002471"
     CDS             6236..7882
                     /gene="mqo"
                     /locus_tag="K0W68_002471"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HBI3074003.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8191..8679)
                     /gene="eco"
                     /locus_tag="K0W68_002472"
     CDS             complement(8191..8679)
                     /gene="eco"
                     /locus_tag="K0W68_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBI3074004.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(8814..8978)
                     /locus_tag="K0W68_002473"
     CDS             complement(8814..8978)
                     /locus_tag="K0W68_002473"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074005.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            9087..9581
                     /gene="napF"
                     /locus_tag="K0W68_002474"
     CDS             9087..9581
                     /gene="napF"
                     /locus_tag="K0W68_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBI3074006.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9571..9834
                     /gene="napD"
                     /locus_tag="K0W68_002475"
     CDS             9571..9834
                     /gene="napD"
                     /locus_tag="K0W68_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBI3074007.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9831..12317
                     /gene="napA"
                     /locus_tag="K0W68_002476"
     CDS             9831..12317
                     /gene="napA"
                     /locus_tag="K0W68_002476"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBI3074008.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12324..13019
                     /gene="napG"
                     /locus_tag="K0W68_002477"
     CDS             12324..13019
                     /gene="napG"
                     /locus_tag="K0W68_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBI3074009.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            13006..13869
                     /gene="napH"
                     /locus_tag="K0W68_002478"
     CDS             13006..13869
                     /gene="napH"
                     /locus_tag="K0W68_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBI3074010.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13866..14315
                     /gene="napB"
                     /locus_tag="K0W68_002479"
     CDS             13866..14315
                     /gene="napB"
                     /locus_tag="K0W68_002479"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBI3074011.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14325..14927
                     /gene="napC"
                     /locus_tag="K0W68_002480"
     CDS             14325..14927
                     /gene="napC"
                     /locus_tag="K0W68_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBI3074012.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14946..15563
                     /gene="ccmA"
                     /locus_tag="K0W68_002481"
     CDS             14946..15563
                     /gene="ccmA"
                     /locus_tag="K0W68_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBI3074013.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15560..16222
                     /gene="ccmB"
                     /locus_tag="K0W68_002482"
     CDS             15560..16222
                     /gene="ccmB"
                     /locus_tag="K0W68_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBI3074014.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            16264..17001
                     /gene="ccmC"
                     /locus_tag="K0W68_002483"
     CDS             16264..17001
                     /gene="ccmC"
                     /locus_tag="K0W68_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBI3074015.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16998..17207
                     /gene="ccmD"
                     /locus_tag="K0W68_002484"
     CDS             16998..17207
                     /gene="ccmD"
                     /locus_tag="K0W68_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBI3074016.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            17204..17683
                     /gene="ccmE"
                     /locus_tag="K0W68_002485"
     CDS             17204..17683
                     /gene="ccmE"
                     /locus_tag="K0W68_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBI3074017.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17680..19623
                     /gene="ccmF"
                     /locus_tag="K0W68_002486"
     CDS             17680..19623
                     /gene="ccmF"
                     /locus_tag="K0W68_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBI3074018.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19620..20177
                     /gene="dsbE"
                     /locus_tag="K0W68_002487"
     CDS             19620..20177
                     /gene="dsbE"
                     /locus_tag="K0W68_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBI3074019.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            20174..21226
                     /gene="ccmH"
                     /locus_tag="K0W68_002488"
     CDS             20174..21226
                     /gene="ccmH"
                     /locus_tag="K0W68_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBI3074020.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21261..21908)
                     /gene="narP"
                     /locus_tag="K0W68_002489"
     CDS             complement(21261..21908)
                     /gene="narP"
                     /locus_tag="K0W68_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBI3074021.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            22228..>22595
                     /locus_tag="K0W68_002490"
     CDS             22228..>22595
                     /locus_tag="K0W68_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI3074022.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         5058 a         5668 c         6428 g         5441 t
ORIGIN      
        1 gcttgatgtt aggtgcttat ttcgccattc cgcaataatc ttaaaaagtt cccttgcatt
       61 tacattttga aatatctata gcgataaatg aaacatctta aaagttttag tatcatattc
      121 gtgttggatt attctgcatt tttggggaga atggacttgc cgactgatta atgagggtta
      181 atcagtatgc agtggcataa aaaagcaaat aaaggcatat aacagagggt taataacatg
      241 aaagttaaag tactgtccct cctggtccca gctctgctgg tagcaggcgc agcaaacgct
      301 gctgaagttt acaacaaaga cggcaacaaa ttagatctgt acggtaaagt agacggcctg
      361 cactatttct ctgacaacaa gtcagaagac ggcgaccaga cctatgtacg tcttggtttc
      421 aaaggcgtaa ctcaggttac tgaccagctg accggttacg gccagtggga atatcagatc
      481 cagggcaata cctctgaaga caacaaagaa aactcctgga cccgtgtggc attcgcaggt
      541 ctgaaattcc aggatgtggg ttctttcgac tacggtcgta actacggcgt tgtttacgac
      601 gtaacttctt ggaccgacgt actgccagaa ttcggtggcg acacctacgg ttctgacaac
      661 ttcatgcagc agcgtggtaa cggcttcgcg acctaccgta acactgactt cttcggtctg
      721 gttgacggcc tgaactttgc tgttcagtac cagggtaaaa acggtagcgt aagcggcgaa
      781 ggcatgacca acaacggtcg tggtgctctg cgtcagaacg gcgacggcgt tggcggatct
      841 atcacttatg attacgaagg cttcggtatc ggtggtgcga tctccagctc caaacgtact
      901 gatgatcaga acagcccgct gtacatcggt aacggcgacc gtgctgaaac ctacaccggt
      961 ggtctgaaat acgacgctaa caacatctac ctggctgctc agtacaccca gacctataac
     1021 gcaactcgcg taggttccct gggttgggcg aacaaagcac agaactttga agctgttgct
     1081 cagtaccagt tcgacttcgg tctgcgtccg tctgtagcat acctgcagtc taaaggtaaa
     1141 aacctgggta ccatcgctgg tcgtaactac gacgacgaag atatcctgaa atatgttgat
     1201 gttggtgcta cctactactt caacaaaaac atgtccacct acgttgacta caaaatcaac
     1261 ctgctggacg acaaccagtt cactcgtgac gctggcatca acactgataa catcgtagct
     1321 ctgggtctgg tttaccagtt ctaatctcga ttgatatcga acaaagggcc tgcgggccct
     1381 tttttcattg ttttcagcgt acaaactcat ttttttggtg tactcttgcg accgttcgca
     1441 tgaggataat cacgtatgga aataagcttt acccgcgtgg cactgctggc tgccgcgctc
     1501 ttctttgttg gttgcgatca aaaaccacaa cccgccaaaa cccacgctac tgaagttacc
     1561 gttctagaag gcaaaaccat gggtaccttc tggcgtgcca gcatcccggg cattgatgcc
     1621 aaacgcagtg ccgaacttaa agaaaagatt cagacccagc tggacgccga cgatcagctg
     1681 ctttcgacct ataaaaaaga ttccgcgctg atgcgcttta acgattcgca aagtttatcg
     1741 ccgtggccgg taagtgaagc gatggccgat atcgtcacca cctcgctgcg cattggcacg
     1801 aagaccgatg gcgcgatgga tataaccgtc gggccgctgg tgaatctgtg gggctttggc
     1861 ccggaacaac agtcggttca aattccgagc caggaacaga tcgatgcgat gaaagccaaa
     1921 accggcttac agcacctgac ggtcattaat cagtcgcatc agcaatatct gcaaaaagac
     1981 ctgccggatt taaatgtcga tctctccacc gtcggtgaag gttatgcggc ggatcacctg
     2041 gcacgcttga tggagcagga agggatttcc cgctatctgg tgtcggtggg cggcgcgctg
     2101 aacagccgtg gtatgaacgg tgaaggcctg ccgtggcggg tagcgattca aaaaccaacc
     2161 gataaagaaa acgcggttca ggctgtggtg gatatcaacg gtcatgggat tagcacctct
     2221 ggcagctacc gtaactatta cgaactggac ggcaaacgtc tttcccatgt tatcgatccg
     2281 caaaccgggc gtcccatcga acacaatctg gtatccgtga cggtgattgc tccgacggcg
     2341 ctggaagccg atgcctggga tactggcttg atggtactcg ggccggagaa agccaaagag
     2401 gttgttcgcc gggaagggct ggcggtttat atgatcacca aagaaggcga tagctttaaa
     2461 acctggatgt caccacagtt taaaagcttc cttatcagcg aaaaaaatta aagcgcaaga
     2521 ttgttggttt ttgcgtgatg gtgaccgggc agactaaagg ctatccttaa ccagggagct
     2581 gattatgaaa aacgccacat gcttaactga cgatcaacgc tggcaatctg tcttagcccg
     2641 cgacccgaat gccgacggcg aattcgtttt cgccgtgcgc accacgggta tcttttgccg
     2701 tccgtcttgc cgcgccagac atgccttgcg ggaaaatgtc tccttctacg caaatgccag
     2761 cgaggcactc gccgccggat ttcgcccctg caaacgttgt cagccagaca aagccaatcc
     2821 ccggcaacat cggttggata aaatcaccca cgcgtgtcga ctgctggaac aggaaacgcc
     2881 tgtaacgctg gaagccttag ccgaccaggt ggcgatgagt ccatttcatc tacatcggtt
     2941 gtttaaagcg actaccggaa tgacgcctaa agcctggcaa caggcctggc gcgctcgccg
     3001 tttgcgcgaa tcgctggcga aaggggagag cgtgacgacg tctattctta acgccggatt
     3061 ccccgacagc agcagttact accgcaaagc cgatgaaacg ctgggcatga cggctaaaca
     3121 attccgtcac ggtggcgaaa atctggcggt gcgttacgcg ctggctgatt gtgagctggg
     3181 tcgttgcctg gtggcagaaa gcgagcgggg gatttgcgcg atattgctgg gcgatgatga
     3241 cgcgacatta atcagcgagt tgcagcagat gtttcccgct gccgacaacg cgcctgccga
     3301 tctgacgttt cagcaacatg tgcgtgaagt gatcgccagc ctcaatcaac gcgatacgcc
     3361 gctgacgtta ccgctggaca ttcgcggcac tgcttttcag caacaagtct ggcaggcact
     3421 gcgcacgata ccttgtggtg aaaccgtcag ttatcagcaa ctggctaacg ccatcggcaa
     3481 accgaaagcg gtacgggccg ttgccagcgc ctgtgccgcc aacaagctgg ctatcgtaat
     3541 accctgtcat cgagtggtcc gtggtgatgg cacactttcc ggttaccgct ggggcgtgtc
     3601 gcgtaaagcg caactgctgc gccgcgaagc tgaaaatgag gagaggtaat gttggatctg
     3661 tttgccgatg ctgaaccgtg gcaagagcca ctggcggctg gtgcggtaat tttacggcgt
     3721 tttgctttta acgctgcgga gcaactgatc cgcgatatta atgacgttgc cagccagtcg
     3781 ccgtttcgcc agatggtcac ccccggggga tataccatgt cggtggcgat gaccaactgt
     3841 gggcatctgg gctggacgac ccatcggcaa ggttatctct attcgcccat tgatccgcaa
     3901 acaaataaac cgtggcccgc catgccacaa agctttcatg atttatgtca acgtgcggct
     3961 acggcggcgg gctatccaga tttccagcca gatgcttgtc ttatcaaccg ctacgctcct
     4021 ggcgcgaaac tgtcgctgca tcaggataaa gacgaaccgg atctgcgcgc gccgattgtt
     4081 tctgtttctc ttggcttacc cgcgattttt caatttggtg gcctgaaacg taatgatccg
     4141 ctcaaacgtt tgttgttgga acatggcgat gtggtggtat ggggcggtga atcgcggctg
     4201 ttttatcacg gcattcaacc gctgaaagcg gggtttcatc cactcaccgg cgactgccgt
     4261 tacaacctga cattccgtca ggcaggtaaa aaagaataaa aataagaatt attattgctg
     4321 tgcgcgcgaa gatgtttaaa ctgcgggctg taattcattg tccgggtttt ctgcatggaa
     4381 cttcttgtac ttgtctggcg gcagtatcgc tggccattta tcagtgtgat ggagctaagc
     4441 ctcgccagtg cggctttagg cattggctta attgctttta tcaatcagcg ccttatcgaa
     4501 acggcggata ccagtctgct ggtgttgccg gagtttctgg gattattgct gctgttgatg
     4561 gcaatcactc tcggatcgca actggcgctc accactttgg ggcatcactt cgtttaccga
     4621 ctgcgtagtg aatttatcaa gcggattctc gatacccacg tcgagcgcat tgaacaactc
     4681 ggtagcgcct cgttgctggc gggattaacc agcgatgtgc gcaatatcac cattgctttt
     4741 gtgcgtctgc cggaactggt gcaggggatc attctcacta tcggttcagc ggcgtatctg
     4801 tggatgctgt cgggcaaaat gttgctggta acggctatct ggatggcggt caccatctgg
     4861 ggcggttttg tgctggtggc gcgggtgtac aaacatatgg cgaccctgcg tgaaaccgaa
     4921 gataagctgt acacggattt tcaaaccgta ctcgaagggc gcaaagagct gactctgaac
     4981 cgtgaacgcg ccgagtatgt gtttaacaac ctctacattc ctgatgcgca agagtatcgc
     5041 catcatatta tccgcgcaga caccttccat cttagtgccg tgaactggtc aaacatcatg
     5101 atgctgggcg ctatcggcct ggtgttctgg atggcgaaca gcctcggttg ggctgatacc
     5161 aacgttgctg cgacctattc gttgacgctg ttattcctgc gtacgccgct gctttcggcg
     5221 gttggcgcat tgccgacgct gctgacggcg caggtggcgt ttaacaagct gaacaaattc
     5281 gcgctcgcgc ctttcaaagc agagtttccg cgcccgcagg cgtttcccaa ctggcaaacg
     5341 ctggagctgc gtaatgtgac gtttagctat caggataacg cgttttccgt tgggccgatt
     5401 aacctcacca ttaaacgtgg cgagctgctg tttctgattg gtggcaacgg tagtggaaaa
     5461 tcgacgctgg cgatgttgct gacgggcttg taccagccac aaagcggcga aatcttgctg
     5521 gatggcaaac ctgtcagcgg cgaacaaccg gaagattatc gcaaactgtt ttcggcagtg
     5581 tttaccgatg tctggctgtt tgatcaactg ctggggccgg aggggcaacc cgctaacccg
     5641 caactggttg agaagtggct ggcgcagctg aaaatggctc ataagcttga gttaagcaac
     5701 gggcgtattg ttaacctgaa gttatcaaaa gggcagaaaa aacgcgtggc gctgttgctg
     5761 gcgctggcag aagaacgcga tattatcctg ctggatgaat gggcggcgga tcaggatcca
     5821 cacttccgtc gtgagtttta tcaggtgttg ctgccgctga tgcaggagat gggtaaaact
     5881 attttcgcca tcagtcatga tgatcattac tttatccacg ccgaccgcct gctggaaatg
     5941 cgcaatgggc aacttagcga gctgacgggc gaagagcgcg atgccgcttc gcgtgatgcc
     6001 gttgcccgga cggcataata ttacgacgct gacctggcgg actgctgccg ccaggtcaat
     6061 attctaaaaa tcccttcatt tctaataacc ctataattaa tcaacgaaat tataatgttt
     6121 ctaaaattag aatataattt ataaacatta tttaaatgtt gttactttag tgttaccgtt
     6181 gatgccgcgc aaatctcacc tgcaataaag cgactaaaag taaggcatta acaagatgaa
     6241 aaaagtgact gccatgctct tctcgatggc cgtggggctt aatgccgttt cgatggcggc
     6301 aaaagcgaaa gcgtccgagg agcaggaaac tgatgtactg ttgattggcg gcggcattat
     6361 gagcgccacg ttggggacct atttacgcga gctggagcct gaatggtcga tgaccatggt
     6421 ggagcgcctg gagggtgtcg cgcaggagag ttcgaacggc tggaacaacg ccggaaccgg
     6481 gcattctgca ctgatggaac tgaactacac cccgcaaaac gccgatggca gtatcagtat
     6541 tgaaaaagca gtcgccatta acgaagcatt tcagatttcc cgccagttct gggcgcacca
     6601 ggtcgagcgc ggcgtgctgc gtactccgcg ttcatttatc aataccgttc cgcatatgag
     6661 ctttgtctgg ggcgaggata acgttaattt cctgcgcgcc cgttacgccg cgttgcaaca
     6721 aagctcgctg tttcgcggta tgcgttactc tgaagatcac gcgcagatca aagagtgggc
     6781 accgttagtg atggaagggc gcgatccgca acagaaagtg gcagccacgc gtacggaaat
     6841 tggtaccgat gtgaactacg gcgagatcac ccgccagtta attgcttcct tgcagaagaa
     6901 atctaacttc tcgctgcaac tcagcagcga agtccgcgcc ctaaagcgta atgacgataa
     6961 cacctggacc gttaccgttg ccgatctgaa aaatggcact gcacagaaca ttcgtgccaa
     7021 atttgtcttt atcggcgcgg gcggtgcggc gctgaagctg ttacaggaat cggggattcc
     7081 ggaagcgaaa gactacgccg gtttcccggt gggcggacag ttccttgttt cggaaaaccc
     7141 ggacgtggtt aatcaccatc tggcgaaggt ttacggtaaa gcatccgttg gcgcaccacc
     7201 gatgtcggtt ccgcatatcg atacccgcgt tctggacggt aaacgcgtag tgctgtttgg
     7261 gccatttgcc accttctcaa ccaaattcct caaaaacggt tcattgtggg atctgatgag
     7321 ttccaccacc acctctaacg tgatgccgat gatgcacgtc gggctggata atttcgatct
     7381 ggtgaaatac ttggtgagtc aggtgatgtt gagtgaagag gatcgttttg aagcgttgaa
     7441 agagtactat ccgcaggcga aaaaagagga ctggcgtttg tggcaagcgg ggcagcgtgt
     7501 gcagattatc aagcgtgatg ccgataaagg tggtgtactg cgtctgggta ctgaagtggt
     7561 cagtgaccag caagggacca ttgccgcgct gctgggggca tcgccagggg cgtcaaccgc
     7621 tgcgccgatt atgttggatc tgctggaaaa agtatttggc gatcgcgttt ccagcccgca
     7681 atggcaggct acgttgaaag cgatcgttcc atcttatgga cgcaagctga acggtgatgt
     7741 agcggcaaca gaacgcgagt tgcagtacac cagcgaagtg ctgggcctga aatacgacaa
     7801 gccgcaggca gcagatagta cgccgaaacc gcagttgaaa ccgcaacccg ttcaaaaaga
     7861 agtggcggat attgcgttgt aatgatgtgc cacatccggc atggtatgcc ggatgtggcg
     7921 taaccgcctt atccgaccta cggggtggag cacttcacag cggtgtaggc ctgataagac
     7981 gcgtcagcgt cgcatcaggc aaccggctcg ggcgttagat gtcggatgcg gcgtaaccgc
     8041 cttatccaac ctacggggtg gagcacttca cagcggtgta ggcctgataa gacgcgccag
     8101 cgtcgcatca ggcaaccggc ttgggcgtta gatgtcggat gcggcgtgac cgccttatcc
     8161 gacctacggg gtgacgcact tcacagcagt ttagcgaact accgcgttgt caattttctc
     8221 ttccgccttc cagacgcggt acttcacatc tacattgtct ggcgtataca ccacgatcgg
     8281 cagcttgctg ttgtaacgca gcattccagc atcgcccaga tacgcggtga caaatttctt
     8341 ctctttcttg ccatccgggc aggccatcat cgtggaaacc ggcgagctaa ctttatcaaa
     8401 gacataataa tcatagcccc agccttccag cgttttgttt tccagcttcc cgccgagacg
     8461 atgcaaattg caatcgactt ccagcgtctg accggttaac agttctactt tcagggtaga
     8521 ttcatcttct tgcggggtta actgaatcac ctgacgcttc atcccttttt cagcttgtgg
     8581 ataaggcgcg attttttcca gtggctggac gctttctgcc gcccaggcgg aagtggtggc
     8641 gaaagcggca aacaatactg caggtagaat ggtcttcatt tttttgcttt ccttcttcat
     8701 ttataggttt tcaccgatga tgtcgctgaa ggttaacata ttcatcagat ttattaattt
     8761 gctttatgaa taacttaaag aacattcaga caattttacc tgctctttta gtgttaaatt
     8821 ctgatgaggg agcaaaccag cagaaatatc tctggtgaat taatcgctat ataaatatgt
     8881 ttataaccat ttgaaatgtg agcaaaagcc cgtttttgcc acgcttcccg ccactctttt
     8941 gatcctgcta caggttttac cccgatcggg gtatgcatct ttgacacatc ctttaatatc
     9001 ttagcggcta taaaaatggc ttattaatta tgcggtttat ttggtcgctc tcaattttca
     9061 gagcgcgtta atgatggaag gtcaatgtga agattgatgc atcccgtcgg ggcatactca
     9121 ctggtcgctg gcgcaaagcc agtaacggta tccgtccgcc ctggtccggt gatgaatctc
     9181 attttctgac ccattgcacc cgttgcgacg cctgtattaa tgcttgtgaa aacaacattc
     9241 tgcaacgcgg cgcgggcggc tatccgagcg ttaatttcaa aaataacgag tgcagcttct
     9301 gttacgcctg cgcgcaggct tgccccgaat cgctattttc tccgcgccac accagggcct
     9361 gggatttgca gttcaccatt ggggacgcgt gcctggcgta tcagtcagtt gaatgccgcc
     9421 gctgtcagga tagctgtgag ccaatggcaa ttatctttcg cccgacgctc tccgggatct
     9481 accagccgca acttaatagc caactttgca acggatgtgg agcatgtgca gccagctgtc
     9541 cggtatcagc cataactgcg gagtatctcc atgcacacta actggcaagt ttgcagcctg
     9601 gtcgtgcagg ccaaaagcga acgaatttca gacatcagca cccaactgaa cgcctttccc
     9661 ggctgtgaag ttgctgtcag cgacgcgccg agcggtcagt tgattgtggt ggtggaagca
     9721 gaagacagcg aaacgctgat ccaaaccatt gagtcagtac gcaacgtaga gggcgtgctg
     9781 gcggtgtcgc tggtttatca ccagcaggaa gagcaaggtg aggaaacacc atgaaactca
     9841 gtcgtcgtag ctttatgaaa gctaacgccg ttgcggccgc tgcggcggct gccggtctca
     9901 gcgtgccggg cgttgcccgc gccgttgttg gtcagcagga agccattaaa tgggataaag
     9961 cgccgtgccg tttctgcggt actggttgcg gcgttctggt cggaacgcag caggggcgtg
    10021 tggtggcctg tcagggcgac ccggacgcac cggttaaccg tggcctgaac tgcattaagg
    10081 gctatttcct gcccaagatc atgtacggta aagaccgttt gacgcagccg ctgctgcgta
    10141 tgaaaaacgg taaatatgac aaagaaggcg aatttacccc aatcacctgg gatcaggcct
    10201 tcgatgtgat ggaagagaaa ttcaaaaccg ccctgaaaga aaaagggccg gaatcgatcg
    10261 gtatgttcgg ttctggtcag tggactatct gggaaggtta tgccgcgtcc aagctgttta
    10321 aagcgggctt ccgttcgaac aacatcgacc cgaacgcgcg tcactgtatg gcgtcggcag
    10381 tagttggctt tatgcgtacc tttggtatgg atgagccgat gggctgctat gacgacatcg
    10441 agcaggctga cgcgtttgtg ctgtggggcg caaacatggc ggagatgcac ccgatcctct
    10501 ggtcgcgcat cactaaccgt cgtctctcta atcagaacgt caccgtggcg gtgctttcta
    10561 cctaccagca tcgtagcttc gagctggcgg ataacggcat catctttacg ccacagtctg
    10621 acctggtgat cctgaactac atcgccaact atatcattca aaacaatgcg ataaatcagg
    10681 acttcttcag caaacacgtt aacctgcgca aaggggcgac ggacatcggc tacggtttac
    10741 gtccgaccca tccgctggaa aaagcagcga agaatccagg ttctgacgcc tccgaaccga
    10801 tgagctttga agattacaaa gccttcgttg ccgagtatac gctggaaaaa accgctgaaa
    10861 tgaccggcgt gccgaaagac cagttagaac aactggcgca gctgtatgcc gatccgaaca
    10921 agaaagtcat ctcctactgg acgatgggct tcaaccagca tactcgtggc gtgtgggcta
    10981 acaacctggt ctacaacctg cacctgctga ccggcaaaat ttcccagccg ggttgcggtc
    11041 cgttctccct gaccgggcaa ccttctgcgt gtggtactgc gcgtgaagtg ggcacctttg
    11101 ctcaccgtct gcctgcggac atggtggtga ccaacgagaa acaccgtgat atctgcgaga
    11161 agaagtggaa tatcccgagc ggcaccattc cggcgaaaat cggtctgcac gcggtggcac
    11221 aagaccgtgc gctgaaagac ggcaaactga atgtttactg gaccatgtgt accaacaaca
    11281 tgcaggccgg gccgaacatc aatgaagagc gtatgccggg ctggcgcgat ccgcgcaact
    11341 tcatcatcgt ctccgatccg tatccgacag tcagtgcgct ggccgccgac ttgatcctgc
    11401 cgaccgcaat gtgggtagag aaagagggtg cttacggtaa cgccgaacgc cgtactcagt
    11461 tctggcgtca gcaggtacag gcaccgggcg aagcgaaatc ggatctctgg cagttagtcc
    11521 agttctcccg ccgcttcaaa actgaagaag tgtggccgga agagctgcta gcgaagaaac
    11581 cggaactgcg tggcaaaacg ctgtacgaag tgctttatgc cacaccggaa gtgagcaaat
    11641 tcccggtatc cgaactggcg gaagatcagc tgaacgatga atcccgcgag ctgggcttct
    11701 atctgcaaaa agggctgttc gaagagtacg catggtttgg tcgcggtcac ggtcacgatc
    11761 tcgcgccgtt cgatgactac cacaaagcgc gcggtctgcg ctggccggtg gtgaacggta
    11821 aagaaacgca gtggcgttac agcgaaggta acgacccgta cgtgaaagcg ggcgaaggct
    11881 ataagttcta cggtaaaccg gatggtaaag cggtgatctt cgcgctgccg ttcgaaccgg
    11941 cggcggaagc accggatgaa gagtacgacc tgtggctctc taccggacgc gttctggagc
    12001 actggcacac tggcagtatg actcgccgtg tgccggaact gcaccgcgcc ttcccggaag
    12061 cggtcttgtt tattcacccg ctggatgcga aagcgcgcga tctgcgccgt ggcgacaaag
    12121 tgaaagtggt ttctcgccgt ggcgaagtga tctcgattgt tgaaacgcgc ggtcgtaacc
    12181 gtccgccaca gggcctggtg tacatgccgt tcttcgacgc cgcacagctg gttaacaaac
    12241 taacgctgga cgcgaccgat ccgctctcga aagagacgga cttcaagaag tgcgcagtca
    12301 aactggagaa ggtgtaagcc attatgtccc ggtcagcgaa acctcaaaat ggtcgccgcc
    12361 gctttctgcg cgatgttgtt cgcacagcag gcgggctggc tgccgtgggt gtggcgctgg
    12421 ggttacaaca gcaaaccgca cgcgcatctg gcgtgcggtt gcgcccgccc ggagccataa
    12481 acgagaacgc ctttgccagt gcctgtgtgc gttgcggtca gtgtgttcag gcttgccctt
    12541 acgacacctt aaaactggcg acgctggctt ctggcctgtc ggcgggcacg ccatattttg
    12601 tcgcacggga tattccttgc gaaatgtgtg aggacattcc gtgcgccaaa gtgtgcccga
    12661 gtggtgcgct ggatcgtgag atcgaatcga tcgacgacgc gcggatgggg ctggcggtgc
    12721 tggtggacca ggaaaactgt ctcaactttc aggggctgcg ctgcgatgtt tgttatcgcg
    12781 aatgcccgaa aattgatgag gccatcaccc tggagctgga gcgcaacacg cgtaccggta
    12841 agcacgcccg ctttctgccg acggttcaca gcgacgcctg taccggctgc ggtaagtgcg
    12901 aaaaagtgtg cgtgctggaa caaccggcaa tcaaggtgtt accgctgtca ctggcgaaag
    12961 gggagttagg tcaccattac cgcttcggct ggctggaggg gaacaatggc aaatcgtaaa
    13021 cgtgacgccg ggcgcgaggc gctggtgaaa aaaggctggt ggcgcagtca ccgttggctg
    13081 gtgttacgtc gcctttgtca gttcttcgtg ctggggatgt ttttgagcgg tccgtggttt
    13141 ggtgtgtgga tcttacacgg caactacagc tgtagcctgt tattcgacac cgttccgctg
    13201 accgatccgc tgatgacgct gcaaagtctt gccagtggtc atctgcctgc cacggtggcg
    13261 ttgaccggtg cggtgataat cactgtgctc tacgccctgg caggaaagcg attattttgc
    13321 agctgggtct gcccgctgaa cccgattacc gacttagcga actggttacg caggcgcttt
    13381 gacctcaatc agtctgcgac gatccctcgc cacattcgct acgtgctgtt ggtggtcatt
    13441 ctgctgggat cagccttaac cggcacgctc atctgggaat ggatcaaccc cgtttccctg
    13501 atggggcgta gcctggtaat gggcttcggc agcggcgcgc tgcttattct cgcgctgttt
    13561 ttatttgatc tactggtcgt tgaacacggc tggtgcgggc acatttgtcc ggttggcgcg
    13621 ctgtatggcg tgctgggtag caaaggtgtg attaccgttg cggccagcga tcggcagaaa
    13681 tgtaaccgct gtatggattg ttttcatgtt tgcccggaac cgcatgtgct acgtgccccg
    13741 gtgctggatg aacaaagccc ggtgcaggtc accagccgcg attgcatgac gtgcggtcgc
    13801 tgcgtggatg tctgttctga ggatgtattt acaataacta cacgatggag ttcgggagcg
    13861 aaatcatgaa aagccatgac ctgaagaaag cgctgtgtca atggacggcg atgctggccc
    13921 tggtggtaag cggcgcggtt tgggcggcta atggcgtcga ttttagccag tcgccggaag
    13981 tatccgggac gcaggaaggg gccattcgga tgccaaaaga gcaggatcgg atgccgctga
    14041 actatgtgaa tcagccgcca atgatcccgc atagcgttga aggttatcag gtaacgacca
    14101 ataccaaccg ctgcttgcag tgccacggtg tcgaaagcta tcgcaccact ggcgcgccgc
    14161 gtatcagtcc tactcacttt atggacagcg acggcaaagt gggcgcggaa gtggctccgc
    14221 gtcgttattt ctgtctgcaa tgtcacgtac cgcaggccga taccgcgcca atcgtgggga
    14281 atacctttac cccatcaaaa ggttacggga aataagaggt tattatggga aattctgacc
    14341 gtaagcctgg tctgattaag cgcctgtgga aatggtggcg tacccccagc cgtctggcgc
    14401 tgggaacgct gctgttgatc ggttttgtcg gcggcatcgt cttctggggc ggctttaaca
    14461 ccggaatgga aaaagccaat accgaagagt tctgcattag ctgccacgaa atgcgtaaca
    14521 cggtgtatca ggaatacatg gattccgtgc actacaacaa ccgtagcggc gtccgtgcga
    14581 cctgtccgga ttgtcacgtt ccgcacgagt ttgtgccgaa gatgatacgc aagctcaaag
    14641 caagtaaaga gctgtatggt aaaatttttg gcgttattga cacgccgcag aaatttgaag
    14701 ctcatcgtct gacaatggca cagaatgagt ggcggcgcat gaaggacaat aactcgcagg
    14761 agtgccgtaa ctgtcacaac ttcgagtata tggatacaac cgcccagaaa tcggttgccg
    14821 cgaagatgca tgaccaggcg gtgaaagatg ggcaaacctg tattgattgc cataaaggga
    14881 tagcgcacaa gctgcccgat atgcgtgaag tcgagccagg tttttaacag ggttattgcg
    14941 tgggtatgct tgaagccaga gagttacttt gtgagcggga tgaacgaacc ttatttagtg
    15001 gcttgtcatt tacgctgaac gcaggagagt gggtacaaat caccggtagc aacggcgcgg
    15061 ggaagacaac gcttctccgt ttgctgacgg ggttgtctcg ccctgacgca ggcgaggttc
    15121 tctggcaagg gcagcccttg catcaggtac gcgacagcta ccatcaaaac ctgttatgga
    15181 taggccatca gccggggatc aaaacccggc tgacggcgtt agaaaatctg cacttttatc
    15241 atcgcgatgg cgataccgca caatgtctgg aagccctggc gcaggccggg cttgccggat
    15301 tcgacgatat tccggtaaat caactttctg ccgggcaaca acgccgcgtc gctttagcgc
    15361 gtctgtggct gacccgtgcc acgttatgga tcctcgacga gccttttacc gcgattgacg
    15421 ttaacggcgt cgatcgtctg acccagcgta tggcgcagca tacggagcag ggggggattg
    15481 tgattctgac tacccaccag ccgctcaacg ttgctgaaag taaaattcgc cgcatttcac
    15541 tgacgcaaac gagggccgca tgatgttctg gcgcattttc cgtcttgagc tgcgtgtagc
    15601 gtttcgccat agcgccgaaa tcgccaaccc gctgtggttc ttcctgattg taattaccct
    15661 ttttccgctc agtatcggtc cggagccgca actgctggcg cgtattgcac cgggcattat
    15721 ctgggttgct gcgctgcttt catccttgct ggcgctggaa cgactgttcc gtgacgattt
    15781 gcaggacggc agtcttgaac aattgatgtt gttgccgtta cccttgcccg ccgttgtgct
    15841 ggcgaaggtg atggcgcact ggatggtaac cggtctgccg ttactcatcc tttcgccact
    15901 ggtagcaatg ctactgggaa tggatgttta tggctggcaa gtgatggcgc tgacgctgct
    15961 gctgggaacg cctacgcttg gctttctcgg tgcaccgggt gtggcgttga cagtgggact
    16021 taagcgcggt ggtgtgctgc tcagcatact ggtgttaccg ctgactatcc cattactcat
    16081 ctttgccacc gccgcgatgg acgcggcttc catgcatttg cccgttgacg ggtatctggc
    16141 aattttaggc gcgttgctgg caggcaccgc gacattaagt ccttttgcga cggcggcagc
    16201 gttacgaatc agcattcaat aacgctggat tactgatttt tgttcgagtc tggtatcgaa
    16261 actatgtgga aaacactgca tcaactggcg atcccaccac ggctgtatca aatctgtggc
    16321 tggtttatac cgtggctggc aattgccagt gtggtcgtgc ttaccgtcgg ctggatctgg
    16381 ggattcggct ttgctccggc tgattatcag cagggaaata gctaccgcat tatctacctg
    16441 catgtgcctg cggcgatctg gtcgatgggc atttatgcat caatggcagt ggcagcgttt
    16501 attggccttg tctggcagat gaaaatggcc aacctggcgg tggcggcgat ggcccccatt
    16561 ggtgccgtgt ttacctttat tgccctggtt accggctctg catggggaaa accgatgtgg
    16621 ggcacctggt gggtatggga tgcacgtctg acttctgaac tggtgctgct gtttttgtat
    16681 gtgggtgtga ttgccctgtg gcacgccttc gacgaccgcc gtctggcggg ccgtgcggca
    16741 ggtatcctgg tgctgattgg cgtggtgaat ctgccgatta ttcattactc cgtggagtgg
    16801 tggaacaccc tgcatcaggg atcaacgcgg atgcagcaaa gtatcgatcc ggcgatgcgt
    16861 tcgccgctgc gctggtcgat ttttggcttc ctgctcctgt ctgccacgct gacgctgatg
    16921 cggatgcgta atttgatttt gctgatggaa aaacgccgtc cgtgggtgag tgaactgata
    16981 ctgaaaagag gccgtaaatg acccctgcat ttgcttcctg gaatgaattt ttcgcaatgg
    17041 gcggttacgc cttttttgtc tggctggcgg tggtgatgac cgttattccg ctggtggttt
    17101 tggtcgtgca ctcggtgatg caacatcgcg caattctgcg tggtgtggcg caacagcggg
    17161 cgcgtgaggc gcgtttacgt gctgcgcaac agcaggaggc tgcatgaata ttcgccgtaa
    17221 aaaccgcttg tggattgcct gtgccgtgtt ggcagggctg gcgctgacta tcggtctggt
    17281 gctatatgcg ctgcgctcga atatcgatct cttttatacg ccgggggaaa ttctctacgg
    17341 caagcgtgaa actcagcaaa tgccggaagt cggtcagcgt ttgcgcgttg gcgggatggt
    17401 gatgctgggt agtgtgcagc gcgatcccaa ttcgctgaaa gtgaccttca ctatttacga
    17461 tgctgaaggc tcagtggatg tctcttacga aggcattttg ccggatctgt tccgtgaagg
    17521 gcagggcgtt gtggtgcagg gcgagctgga aaaaggcaat catatcctcg cgaaagaagt
    17581 gctggcgaaa cacgacgaaa actacacgcc gccagaagtt gagaaagcga tggaagctaa
    17641 ccaccgtcgc ccggcgagtg tttataagga cccagcatca tgatgccgga aattggaaac
    17701 gggctgctgt gtctggcgtt agggattgcg ctgctgctgt ccgtgtatcc gctatggggc
    17761 gtggcgcgcg gagatgcgcg catgatcgcg tcttcccgct tgtttgcctg gctgctgttt
    17821 atgtctgtgg ctggcgcatt tctggtgctg gtcaatgctt tcgtggtcaa cgactttacc
    17881 gtcacctatg ttgccagcta ctccaatacc cagcttccgg tgtggtatcg cgtggcggct
    17941 acctggggcg cgcatgaagg ctcgctactg ctgtgggtgc tgctgatgag cggctggacc
    18001 tttgcggtgg cgatttttag tcagcgtatt ccgctggata ttgtggcccg cgtactggcg
    18061 ataatgggga tggtcagcgt cggctttttg ctgttcattc tctttacctc taacccattc
    18121 tcacgcacgc tgccgaactt cccgattgaa gggcgcgatc ttaacccgct gttacaggac
    18181 ccggggctga tcttccatcc gcctctgctt tatatggggt acgtgggttt ctcggtggcg
    18241 tttgcttttg ccattgcttc tttgctgagc gggcgtctgg acagcactta tgcgcgtttt
    18301 actcgcccgt ggacgctggc ggcgtggatt ttcctgacgc tcggcattgt gctcggttcc
    18361 gcatgggcct attacgaact cggctggggg ggctggtggt tctgggatcc ggtagaaaac
    18421 gcctcgttta tgccgtggct ggtggggact gcgctgatgc actcactggc ggtcactgaa
    18481 caacgcgcca gcttcaaagc gtggacatta ctgctggcaa tcagtgcctt ctcgttgtgt
    18541 ctgttgggga ctttcctcgt gcgttccggc gtgctggtat cggtacacgc gtttgcgtct
    18601 gatccggcgc gcggtatgtt tatcctcgcc tttatggtgc tggtgattgg aggttcgctg
    18661 ctgctgtttg ccgcgcgtgg acacaaagtt cgctcacgcg taaacaatgc gctgtggtcg
    18721 cgggaatctc tgctgttagc gaacaacgtt ttgctggtcg ccgcgatgct ggtggtgttg
    18781 ctggggacgc tgctgccgct ggtgcacaag caactgggac tgggcagtat ttcgattggc
    18841 gaaccgttct tcaacaccat gtttacctgg ctgatggtgc cgtttgcgct actgctgggt
    18901 gtcggtcctc tggtgcgctg ggggcgcgat cgcccacgta aaatccgcaa tttattgatt
    18961 atcgccttca tctcaacgct ggtgctgtct ctgctgttgc cgtggctgtt tgagagcaaa
    19021 gttgtggcga tgacggtgct cggcctggca atggcctgct ggattgcggt gctggcaatt
    19081 gcggaagctg cgctgcgtat ttcacgcggc acgaaaacca ccttcagtta ttgggggatg
    19141 gtggctgctc acctggggct ggcagtgaca attgttggca ttgcttttag ccagaactat
    19201 agcgttgagc gtgatgtgcg catgaagtcc ggcgatagca tcgatattca tgaatatcgc
    19261 ttcaccttcc gtgatgtcaa agaggtgact ggcccgaact ggcgtggcgg tgtggcgact
    19321 atcggcgtaa cgcgagaggg taaaccggaa acagtgctgt atgcggaaaa acgttattac
    19381 aacactgccg ggtcgatgat gaccgaagcg gcgattgacg gtggcatcac gcgtgacctg
    19441 tacgccgcgc tcggtgaaga gctggaaaac ggcgcgtggg ctgtgcgtct ttactacaaa
    19501 ccatttgttc gctggatttg ggcgggcggg ctgatgatgg cgttgggcgg actgctgtgt
    19561 ctgtttgatc ctcgctatcg taagcgcgtg agtccgcaaa aaactgcgcc ggaggctgta
    19621 tgaagcgcaa agtattgtta attccgttga ttatcttcct ggcgattgcc gcggcgctgc
    19681 tgtggcagct ggcgcgtaat gccgaagggg atgatccgac caatctggaa tcggcgctca
    19741 ttggcaagcc agtgccgaag tttcgcctcg aatcactgga caatccgggg cagttttatc
    19801 aggcggatgt gctgactcag ggcaaaccag tgctgcttaa cgtctgggcg acctggtgtc
    19861 cgacctgccg tgcggaacat caatatctga atcagctttc tgcgcagggc atccgcgtag
    19921 tcggcatgaa ctataaagac gatcgccaga aggcaatcag ctggctgaaa gagctgggca
    19981 atccttacgc gctaagcctg tttgatggcg atggcatgtt agggctggat ctcggtgtct
    20041 atggcgcgcc agaaacgttc cttattgacg gcaacggcat cattcgctat cgccatgcgg
    20101 gcgatctcaa tcctcgcgtc tgggaagaag agatcaagcc gctgtgggag aaatacagta
    20161 aggaggccgc acaatgaggt ttttattggg catgctgatg ctgatgatct ccggctcagc
    20221 gctggcgacc atagacgtgt tgcagtttaa agatgaagca caggaacaac agttccgtca
    20281 gctcactgaa gaactgcgct gcccgaaatg ccagaacaac agcattgccg attccaactc
    20341 gatgattgcc accgacctgc gccagaaagt gtatgaactg atgcaggaag gtaaaagtaa
    20401 aaaagagatt gtcgattata tggtggcgcg ttacggcaac ttcgtcactt acgatccgcc
    20461 gttaacgccg ctgaccgtgc tgctgtgggt gctgccagta gtagctattg gcattggcgg
    20521 ttgggtcata tacgcccgct cgcggcgtcg ggtacgcgtg gtgccggaag cgtttcctga
    20581 acaaagcgtg ccggaaggta agcgtgccgg atatgtcgct tatttgccgg gcattgtggt
    20641 ggcgttaatt gtggctggcg tcagctacta ccagaccgga aattatcaac aggtgaaaat
    20701 ctggcagcag gccacggcac aggctccggc gttgctggac agggcgctgg atccgaaagc
    20761 tgatccgctc aacgaagaag agatgtcgcg ccttgcgctg gggatgcgta ctcaactgca
    20821 aaaaaatccg ggagatatag acggctggat tatgttgggc cgcgttggca tggcgctggg
    20881 taacgccagt atcgccaccg atgcatacgc tactgcgtat cgcctcgatc cgaaaaacag
    20941 tgatgccgca ctgggttatg ctgaagcgtt gacacgttca tctgatccca acgacaaccg
    21001 cctcggcggc gaactgctac gtcagctggt gagaacggac catagcaata tccgtgtgct
    21061 aagcatgtat gcgtttaatg cctttgagca gcagcgattt ggcgaagccg ttgccgcgtg
    21121 ggagatgatg ttgaaactct tacctgccaa cgatactcgc cgtgcggtga ttgaacgtag
    21181 tatcgcgcag gcgatgcaac atttgtcgcc gcaggagagt aaataaaaag aggcccgatg
    21241 gtgatgccat cgggctattt ttattgtgcc ccgcgttgtt gcaggaacag aatggtggcc
    21301 gccacgcgtg agcggacatt gagtttacgc agcagatttc gaatatgaac ttttactgtc
    21361 tgctcggaaa tattcaacac agaggcaatc tgtttatttg acagcccctg tgccagctcg
    21421 tgcagaacat ccagctcgcg ctccgtcagc acgctgaagg gatcttcttc cgcgccaaac
    21481 atttcacgtt cacgtaagta ctgattgacg cgttcgctaa agactttgct gcctttcgct
    21541 ccggcacgaa tcgcttccag caacacttcc gggtcgctgt ctttcagcag ataaccgtct
    21601 gcgcctgcgt ctatcagcgc aaagacatcg ctggaggcat cggatacggt caggataata
    21661 atttgcgcgg taacgccatc cctgcgcaag gcattgaggg tatccaggcc actcatacct
    21721 ttcatattga gatccagcaa gatcacgtcg atatccagtc tattcgccag atcgatcgcg
    21781 ctcgcgccgt cgcccgcttc ggcgaccact tcaaagccag gatcaagctc cagtaactga
    21841 cgaacaccgc gtcgcataag tggatgatca tccacaatca tcacctgaaa aggcgttgct
    21901 tcaggcatag tagtctcctg aggttttatt agaatcatta tgtttgagcg tagcgttggt
    21961 aacgctacca ggcgcgtgaa tgttacccca ttttatgtat ggacatgtag tgaaaactac
    22021 aaccttgccg ttcgctttgg catcattttc attataaaga cggaattttg catcagcttc
    22081 tcatcaagaa aaacaactaa aaaatcaatc acaatacttg tgcatttctt taaacagttt
    22141 ttaaaaactg actccgggta tggagctatg ggtattttct gtacccaatg cttttaacag
    22201 caattaattt cataggatga aagctcaatg catcaatctg gttctgtttc tctttgtcgt
    22261 tccgcaatat ctgttctggt ggctacagcg ttatattcac ccatagcatt ggcatcaaca
    22321 gttgagtatg gtgagacagt tgatggtgtt ggcctggaaa aagatatcca gctggtttat
    22381 gggaccgcca ataatacgaa aatcaatcct ggcggagaac agcatataaa agaatttggt
    22441 gtaagtagta atactgaaat taacggcggg tatcagtaca ttgaaatgaa tggcactgca
    22501 gaatactcag tattaaatga tggttatcaa attgttcaaa tgggtggcgc ggtaaaccag
    22561 actacgctca ataatggagt gctacaggtt tatgg
//
LOCUS       DAETQN010000065        22139 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.065, whole genome shotgun sequence.
ACCESSION   DAETQN010000065 DAETQN010000000
VERSION     DAETQN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22139
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.065"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..524)
                     /locus_tag="K0W68_002491"
     CDS             complement(<1..524)
                     /locus_tag="K0W68_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBI3074023.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQR"
     gene            820..1596
                     /gene="exuR"
                     /locus_tag="K0W68_002492"
     CDS             820..1596
                     /gene="exuR"
                     /locus_tag="K0W68_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBI3074024.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1941..2603
                     /locus_tag="K0W68_002493"
     CDS             1941..2603
                     /locus_tag="K0W68_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3074025.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            2607..2990
                     /gene="mzrA"
                     /locus_tag="K0W68_002494"
     CDS             2607..2990
                     /gene="mzrA"
                     /locus_tag="K0W68_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBI3074026.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3137..3505
                     /locus_tag="K0W68_002495"
     CDS             3137..3505
                     /locus_tag="K0W68_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HBI3074027.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            3543..3848
                     /locus_tag="K0W68_002496"
     CDS             3543..3848
                     /locus_tag="K0W68_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBI3074028.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3851..4255
                     /locus_tag="K0W68_002497"
     CDS             3851..4255
                     /locus_tag="K0W68_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBI3074029.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4245..4544
                     /locus_tag="K0W68_002498"
     CDS             4245..4544
                     /locus_tag="K0W68_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBI3074030.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4730..5122
                     /locus_tag="K0W68_002499"
     CDS             4730..5122
                     /locus_tag="K0W68_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBI3074031.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5192..6205
                     /gene="yqjG"
                     /locus_tag="K0W68_002500"
     CDS             5192..6205
                     /gene="yqjG"
                     /locus_tag="K0W68_002500"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBI3074032.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESTEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPQDEMFASVKKRHLT
                     KCL"
     gene            6470..6835
                     /locus_tag="K0W68_002501"
     CDS             6470..6835
                     /locus_tag="K0W68_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBI3074033.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7077..7433
                     /locus_tag="K0W68_002502"
     CDS             7077..7433
                     /locus_tag="K0W68_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBI3074034.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(7484..8380)
                     /gene="yhaJ"
                     /locus_tag="K0W68_002503"
                     /pseudo
     CDS             complement(7484..8380)
                     /gene="yhaJ"
                     /locus_tag="K0W68_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            8485..9186
                     /locus_tag="K0W68_002504"
     CDS             8485..9186
                     /locus_tag="K0W68_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBI3074035.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9209..9373
                     /locus_tag="K0W68_002505"
     CDS             9209..9373
                     /locus_tag="K0W68_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074036.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(9507..10817)
                     /locus_tag="K0W68_002506"
     CDS             complement(9507..10817)
                     /locus_tag="K0W68_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBI3074037.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10845..12176)
                     /locus_tag="K0W68_002507"
     CDS             complement(10845..12176)
                     /locus_tag="K0W68_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBI3074038.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(12451..13815)
                     /gene="tdcG"
                     /locus_tag="K0W68_002508"
     CDS             complement(12451..13815)
                     /gene="tdcG"
                     /locus_tag="K0W68_002508"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBI3074039.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13887..14276)
                     /locus_tag="K0W68_002509"
     CDS             complement(13887..14276)
                     /locus_tag="K0W68_002509"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBI3074040.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(14290..16584)
                     /gene="tdcE"
                     /locus_tag="K0W68_002510"
     CDS             complement(14290..16584)
                     /gene="tdcE"
                     /locus_tag="K0W68_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBI3074041.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(16618..17826)
                     /gene="tdcD"
                     /locus_tag="K0W68_002511"
     CDS             complement(16618..17826)
                     /gene="tdcD"
                     /locus_tag="K0W68_002511"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBI3074042.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17852..19183)
                     /gene="tdcC"
                     /locus_tag="K0W68_002512"
     CDS             complement(17852..19183)
                     /gene="tdcC"
                     /locus_tag="K0W68_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBI3074043.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(19205..20194)
                     /gene="tdcB"
                     /locus_tag="K0W68_002513"
     CDS             complement(19205..20194)
                     /gene="tdcB"
                     /locus_tag="K0W68_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBI3074044.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(20293..21231)
                     /gene="tdcA"
                     /locus_tag="K0W68_002514"
     CDS             complement(20293..21231)
                     /gene="tdcA"
                     /locus_tag="K0W68_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBI3074045.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            21420..21764
                     /gene="tdcR"
                     /locus_tag="K0W68_002515"
     CDS             21420..21764
                     /gene="tdcR"
                     /locus_tag="K0W68_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HBI3074046.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         5805 a         5650 c         5169 g         5515 t
ORIGIN      
        1 cgttgattcc ctgcattaac taatttttgg gcagcattta cttctaactt agcaataggt
       61 tgctgaggtg gaacactaac aacaatcccc caaattaagt taactgttaa ctgcatagag
      121 agatcttttt tatctgtggc aacgtcatca atactgggaa ccgcttcaaa tttaacgcga
      181 taattttttt ctttttgctc tggttccatt gcaacaaagc gcacagtttt gctactaccg
      241 gctggtaaag cgaatttagg tggcataaca accagatgat ttgcatcacc ggatttaatc
      301 tcaacttcat tttcctgagg cgtcgatgga ttatcgatac ggaatactgt cgccttgatg
      361 tactgaactt cattactttt agatattacg cgaacgttgc cttcaccctg actattaatg
      421 gaaacagcca taggataaac agtcatattt gactgtgcca aaaaaggagc gaacacgaat
      481 aaaagcggcg taagccgtga taagagatta attttcgcca ccatataaat ttgatcttac
      541 aatattgttt ccaaaaagtt ggtgtattag agatgtagat ttattgtaag tcaatataaa
      601 aaagcatttt tagttaatat taaatttaaa ggctgggaat taacttactg aaatttagta
      661 gctttcacaa tgacgatact tattttttag gaatttataa atattcacaa tattaattca
      721 atacattaat tcaatcacca aatttatata tgaccaatgg catacccatg aataaagtgg
      781 tatgataact tcgtccgcat tccactccgg gagacgcata tggaaatcac tgaaccacgc
      841 cgtttgtatc aacaacttgc cgctgacctg aaagagcgca ttgaacaggg cgtctatctg
      901 gtgggtgata aactgcctgc cgaacgcttt attgccgatg aaaagaacgt cagccgaacg
      961 gtagttcgtg aagccatcat catgctggaa gttgaaggct atgtggaggt gcgtaaaggt
     1021 tcaggtattc atgtggtttc caaccagccg cgccatcagc aggcggctga caataatatg
     1081 gaattcgcca attacggtcc gtttgagttg cttcaggctc gccagctcat cgaaagtaat
     1141 attgccgagt tcgcggcaac tcaggtaacg aaacaagaca tcatgaaact gatggccatt
     1201 caggaacagg cgcgcggcga acaatgcttc cgtgattccg agtgggattt acagttccac
     1261 attcaggtag ccctggcgac gcagaactcc gccctggcgg ccatcgttga aaaaatgtgg
     1321 acccagcgta gccataaccc gtactggaaa aaactacacg aacacattga ttcccgtacc
     1381 gttgataact ggtgtgatga ccacgatcaa attctcaagg cgttaattcg caaagatccc
     1441 catgctgcta agctggcaat gtggcagcat ctggaaaaca ccaaaatcat gttatttaac
     1501 gaaaccagcg acgatttcga gttcaatgcc gaccgctatc tattcgccga aaacccggta
     1561 gtgcatctcg atacggccac cagtggcagt aaatgaaact cctgtccgac aggcgctgcg
     1621 acgcgcctgt tcgcttcgtc ggtaagcgaa aagtataaag tgtcagcctg tgtaaatcct
     1681 ctcgccaccc tcccctgcat tcagcaaaat cagactccac ggacatgtaa ttttgataac
     1741 gaacaacgtt gacctttgtt acaattagat tcaatttgaa tttatgtttt tgaatgcttt
     1801 cttatctcac gatttaacag ggaatagttc aggctgtgtt gatgtatcaa acccgcagaa
     1861 cataccaaaa cagcaataac attgcggtag tgcatctttt aaaaccagcg tggcgttaac
     1921 cgattcacca ggaataatga atggaacttt tgacccaatt gctgcaagcc ctgtgggcgc
     1981 aggattttga aaccctggcc aatccatcga tgattggcat gttgtatttt gtcttgtttg
     2041 taattttgtt ccttgaaaac ggcttgcttc cagcggcctt tttaccgggc gacagtttac
     2101 tggtattggt cggcgtgttg attgcgaaag gcgcgatggg ttacccgcaa acgattctgc
     2161 tgctgaccgt tgccgccagc ctcggctgct gggtcagcta tattcagggg cgatggctgg
     2221 gcaatacccg caccgtacaa aactggctat ctcatttacc cgcgcattat catcaacgcg
     2281 cacaccatct ttttcataaa cacggtttat cggcgctgtt aattggtcgc tttattgcgt
     2341 ttgtcagaac actgctgccg acgattgccg ggttatcagg gctgaataac gcgcgctttc
     2401 agtttttcaa ctggatgagc ggtctgctgt gggtattgat cctgacaact ctgggttaca
     2461 tgctcggcaa aacgccgata tttttaaagt acgaggacca gctgatgtca tgcctgatgc
     2521 tgctcccggt ggtgctgctg gtgtttggcc tggcaggttc tctggtcgtg ttatggaaaa
     2581 agaaatatgg aaatcggggg taagggatgc aaatacctcg catgtcgctt cgccagctag
     2641 cctggtccgg cactgtttta cttctggtcg gcacgctgtt gctggcctgg tccgcggttc
     2701 gccagcaaga gtctacgctg gcgattcgtg ccgttcatca aggcacaacg atgccagacg
     2761 gtttttcgat ctggcatcac cttgacgctc atggcattcc tttcaaaagt atcaccccca
     2821 aaaacgacac tctgttaatt acatttgact ccagcgacca gagcgccgcc gcaaaagcgg
     2881 tcctcgacag aacattgccc catggctaca tcattgcgca gcaggacaat aacagtcagg
     2941 ctatgcagtg gctgacccgg ttacgggata actctcatcg cttcggataa cttcctgtta
     3001 tccgaaacat atcactcact ttggtgattt caccgtaact gtctatgatt aatgaggcgg
     3061 tggttaacac taccgcttcg ttgtctggac aatcgttcct ttgtaatagg tccgaatcac
     3121 aatggaaggt tcaagaatga aataccgcat cgctttagct gtttctctct ttgctcttag
     3181 tgccggtagt tatgccacta ccctgtgtca ggaaaaggag caaaatatcc ttaaggagat
     3241 cagctatgcc gaaaaacacc aaaaccagaa tcgtattgac ggtctgaata aagccctgag
     3301 tgaagtccgg gccaactgtt cagatagcca gctgcgtgcc gatcatcaca agaaaatcgc
     3361 aaagcagaaa gatgaggtgg cggaacgcca gcaagattta gccgaggcga agcaaaaagg
     3421 cgatgccgat aagattgcca aacgcgaacg gaaactggca gaagcgcagg aagagctgaa
     3481 aaagctggaa gcgcgcgact actaactcac aatagtcact acttactcac ctggagaaaa
     3541 ctatgtcgaa agaacacact acggaacatc tgcgtgctga gttgaaatcc ctttccgata
     3601 cgctggaaga ggtgcttagc tcatctggcg agaagtcgaa agaagagttg agtaagattc
     3661 gtagcaaagc ggagcaggca ctgaaacaga gccgttatcg cctgggtgaa accggtgatg
     3721 ccatcgccaa acaaacccgt gtcgcggcgg cgcgtgctga tgagtatgtg cgtgaaaatc
     3781 cgtggaccgg cgtgggaatt ggcgctgcaa tcggtgtagt gctcggcgtt ctgctgtcgc
     3841 gtcgttaatt atggcggaca ctcatcacgc gcaagggccc ggtaaaagcg ttctgggcat
     3901 cgggcagcga attgtttcca tcatggttga aatggtggaa acacgtctgc ggctggcggt
     3961 ggtggagctg gaagaggaaa aagctaatct ctttcaactt ttactgatgc tgggcctgac
     4021 gatgcttttc gctgcatttg gtcttatgag cctgatggtg ctaattattt gggcggttga
     4081 tccgcaatat cgcctgaatg cgatgattgc caccaccgtg gtgttgctgc tactggcact
     4141 gattggcggt atctggacgt tacgtaaatc gcgtaaatcc accttgctgc gccatacacg
     4201 ccatgagctg gcaaacgatc ggcaactgct cgaggaggag tcccgtgagc agtaaagtcg
     4261 aacgtgaacg acgtaaggcg caactgctta gccagatcca gcaacaacgg ctggatcttt
     4321 ccgccagtcg tcgtgaatgg ctggaggcaa caggcgctta cgatcgtcgc tggaatatgc
     4381 tgctaagtct gcgctcctgg gcgctggttg gcagtagcgt gatggcgatc tggacgattc
     4441 gccatcctaa tatgctggtc cgctgggcca gacgcggttt tggcgtatgg agcgcctggc
     4501 gtctggttaa aacgaccctc aagcagcaac agcttcgcgg ttaacttccc tctggccgga
     4561 gcaattccgg ccttatccct caaatttttt gaagattttt gacagttttc cttgctaaca
     4621 atcatcattc accacgttta tgattctctc catcgacagc aacgacgcta ataccgcgcc
     4681 cttgcacaaa aaaacaatca gcagcctgag tggcgcagtg gagagtatga tgaaaaaatt
     4741 agaagatgtt ggtgtactgg tagcgcgcat tttaatgccg attctgttta ttaccgcagg
     4801 ctggggaaaa atcaccgctt actctggtac ccaacaatat atggaagcaa tgggtgtccc
     4861 gggttttatg ctgccgctgg tgattctgct tgagttcggt ggtggtctgg caatcctgtt
     4921 cggtttcctg actcgcacca cagccctgtt tactgcgggc tttacgctgc tgacggcatt
     4981 tttatttcac agcaactttg ctgaaggcgt caactcgctg atgttcatga aaaacctgac
     5041 aatttctggc ggattcctgc tgctggcaat taccggtccg ggcgcgtata gcatcgaccg
     5101 cctgctgaat aaaaagtggt aagcacgcgc tatacttaac gataaaaaac gaggaggaag
     5161 ctcctcgttt ttgctattgg aggagagaaa aatgggtcaa ctgattgacg gcgtctggca
     5221 tgacacctgg tacgatacca aatctaccgg cggtaaattt caacgttcag cttccgcatt
     5281 tcgtaactgg ctcactgccg atggcgcacc tggccccact ggcacaggcg gttttatcgc
     5341 agagaaagat cgttatcatc tctatatttc actcgcctgc ccgtgggcgc accgcacgct
     5401 gatcatgcgc aaactcaaag gactggaacc gtttatttcc gtttccgtag tgaacccgct
     5461 gatgctggaa aacggctgga cctttgatga cagttttccg ggagcaaccg gagatacgct
     5521 ctatcaacat gaatttctgt atcagcttta tctccacgcc gatccacact acagcggacg
     5581 agttactgtt cccgtactgt gggacaaaaa gaaccacacc atcgtcagca acgaatcaac
     5641 agaaatcatc cgcatgttca ataccgcgtt tgatgcgctg ggcgcgaaag cgggtgatta
     5701 ctacccacca gccctgcaaa cgaaaattga cgaacttaac ggctggattt atgacaccgt
     5761 taacaacggc gtgtataaag ctggttttgc caccagccag caagcctacg acgaggcggt
     5821 ggcgaaagtg tttgaatcgc tggcgcgact ggagcagatt ttaggccagc atcgttacct
     5881 gaccggcaac cagctaaccg aagccgatat tcgcctgtgg actacgctgg tgcgttttga
     5941 tccagtgtat gtgacccact tcaagtgtga taagcaccgc atcagcgatt acctgaatct
     6001 gtatggcttc ctgcgcgata tctaccagat gccggggatc gccgaaactg tcaatttcga
     6061 tcacatccgt aatcattact tccgcagcca taagaccatc aaccctacgg ggattatttc
     6121 aattggtccg tggcaggatc tcgatgaacc gcaggacgag atgttcgctt cggttaaaaa
     6181 aaggcacctt acgaagtgcc tctgataatt agctaagtgc atgttgttca tatcggatgc
     6241 ggcgtgaacg ccttatcaaa cctacaaaac atttatattc aatatgttgc aatgaccgcg
     6301 caggccagat aagcgtagcg catctggcga ttttgcgctt gtcatcagtc ttagcgcgcc
     6361 ttcacagcac tttattaatt ccttgaacga atatttactg ccatttcatt cacgtctatt
     6421 cttaatttgc tgctttaaaa acaagtgatt gagcaacatt gaggcaaaaa tggactggta
     6481 tctgaaagta ataaaaaatt atgtcggttt ccggggtcgt gcgcggcgca aagagtactg
     6541 gatgtttatt ctggtcaaca tcatctttac gttcgtgctg gggctgctgg ataaaatgtt
     6601 aggctggcaa cgcgctggtg gcgaaggcat cctgacgaca atctacggta ttctggtgtt
     6661 tttaccgtgg tgggcagttc agttccgccg cctgcacgac accgaccgct cggcgtggtg
     6721 ggcactgcta ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct ttaactgcca
     6781 ggcgggtacg ccaggcgaaa accgctttgg acctgatcca aaactggaac cttaaaacaa
     6841 atctgctcgc cggatgtctg actcatccgg cttttatcat catgttgatg aatgaataat
     6901 ttactaatgt ttatttaaaa tatttcaatg tcgttatata aattacctat aaaaaataac
     6961 catgtattta tcttaaatat caaaaccttt ataacatccg aaaaagaaag atacgaatag
     7021 catttacaca atacccggaa tagactttcg tcacttttaa ttaaagggat gtttttatgc
     7081 agtggtattt atccgtatta aaaaattatg ttggtttctc tggtcgcgca cgtcgtaaag
     7141 agtactggat gtttactctg attaacgcca tcgtcggcgc tattatcaat gtcattcaat
     7201 tgattttagg tctggagctt ccatatctgt ctatgcttta tttgctggca accttccttc
     7261 ccgtccttgc gcttgctatc cgtcgcttac acgataccga ccgttcaggt gcctgggcct
     7321 tgctcttttt tgttccgttt attggctggc tcgtgcttct ggtttttttc tgcacagaag
     7381 gtacttctgg cagcaatcgt tacggaaacg atccgaagtt tggttcaaat taatcttaga
     7441 attggggcga tatttcgccc ctttttatta acaataataa atattatttt ccgctaaaaa
     7501 gtttgggaat ttcccgcaga caccaggatt tcgcttcccc catactgtca cgacgccagg
     7561 ccataataat atcgatctca ctggtcgatt ccgggctgac gacacgcaac cgcccttccg
     7621 caatatcttt ttcgaccatc ggatacggca tcgtcgccac gccaagcccc gccagtaatg
     7681 cctgacgttt atcttcaatc gtgctcaccg ttaagcgcgg ctgtttgtcc agcagttgta
     7741 cggtcaacac cgggcgctca cgagcggtat ccgctaccgc aattccacga tatttcacgc
     7801 gcgtcacttc agataacggt tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt
     7861 agacgttcat taacgtatag agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca
     7921 tatccggcgc gataacaata tccgcccgcc cctgctccag tcgttcctac gcccccgcca
     7981 gcacttcggt gatgattgcc agttgggtat tggcttttgc cgccagtttg tcgattaacg
     8041 ggaaaaaggc aggtgtcggt accagcgctt cggtcacaat ggtgagatgc gtttcccaac
     8101 cgcgcgcgag ggcttccgca tcggtagtca gtttatctgc ggcttccagc aaaacgcgcc
     8161 ctcgctccag caacatcctc ccgacattgg tgaatttggt gcgatggccc gagcggtcaa
     8221 acagcaccac atccagctct tcttccagtt tttgcatggt gtagctaagt gcggaaggca
     8281 cgcgtcccag ctcatccgcc gccgccgcaa aactgccccg gcgatcgatc gcatccataa
     8341 cccgtagtgc ttccagcgtt aatgcccttt ctttggccat ttcgttctca ttcaggaaat
     8401 ttgaacatac cgggcagaat atctggctaa caatgtagcg tccagcccct taccataaaa
     8461 ggaagtaaag agaggtcaag aattatgatt actacccgaa ctgccaggca gtgtggacaa
     8521 gcagactacg gatggttgca ggcccggtat actttttcct ttggacacta cttcgacccg
     8581 aaattgttag gctatgcctc cctgcgtgtg cttaaccagg aagtgctggc cccaggtgcc
     8641 gcctttcagc cgcgaaccta tcccaaagtc gatattttaa atgtgattct ggatggagaa
     8701 gcagagtatc gcgacagcga aggcaatcat gttcaggcca gcgccggtga ggcgttgctg
     8761 ctctctaccc agccgggtgt cagctacagc gaacacaatc tcagcaaaga caaaccgtta
     8821 acacgaatgc agctttggct ggacgcctgc ccgcagcgag agaatccgct gattcaaaaa
     8881 ctggcgctta atatgggcaa gcagcaatta atcgcctcgc cagagggtac gatgggaagc
     8941 ctgcaattac gccagcaagt gtggctgcac catatcgtgc tcgacaaagg cgaaagtgcg
     9001 aatttccagt tgcatgggcc acgcgcgtat ttgcaatcga ttcacgggaa atttcatgcg
     9061 cttacgcatc atgaagagaa agcggcgctg acctgcggtg atggggcgtt tattcgtgac
     9121 gaggctaaca ttacgctggt tgccgattcc ccactgcgcg ctttgctgat agatttgcct
     9181 gtctagttgt ttttacaggg agatgatgat gagtaaaaaa tcggccaaaa agcgccagcc
     9241 agtgaagccc gtggtggcga aagaacctgc tcgcaccgcc aaaaattttg gctatgaaga
     9301 gatgttgagc gagctggaag ccatcgtcgc ggatgctgaa acgcgtttag ccgaggatga
     9361 agctaccgcg taaagacagg ttgccggatg cggcgaaaac gccttatccg gcctacaaaa
     9421 tccaacaaca ttaacgaatt aaacaacccg taggccggat aaggtgcgcc agcatcgcat
     9481 ccggcacaat actcaaaacc tggcgtttat ctggccttgc tcgccataat ctcgataatc
     9541 tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc aatcgactgc
     9601 tcaacatcat gcgccacgat cccttcattg ccggtcacgg cggtatcatc cagcgccatt
     9661 aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca gctgttcgac
     9721 gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc catcgagata
     9781 gtttcataac gcccatccac cagccatgcc atcccggcgg cggcccccat tgctgcggtc
     9841 gttgcggcac aaagtgcaga caaacgcggt aactggttat ggatgtaaat tgcgctcaaa
     9901 tgagaaagca tcagcgcacg cgccagtcgt tcatcatccg ctccgaagtg ttctgctaca
     9961 accaccacag gcattgttgc ggtgatcccc tggttacccg agccggagtt actcatagcc
    10021 ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat cacaatggat
    10081 gaagagagat ctttcgccag caaaccgcgc tcgcactgtt tttccagcgt cgcgccaata
    10141 tgcagccccc acttaccgct caaaccttcc tgcgataacg cacaatttag cttcgcggaa
    10201 tcgagaataa agcggatcgc cgcaaacggg acttcattga cgaacttcag gatctcagcc
    10261 agcgtcgttc tggaaagcac cgtcagcgga gactcttgct cgccctctgc cacacacgcc
    10321 tgctgggtaa acaccacacc attgtgcgtc tcaatacgca caatgttggt atgcccgccg
    10381 acgatggtga cacacgccca cttctcaccg ttccagactt tggcgcgtga gaagaggatt
    10441 tcattgcaag gttcctggat cttaacggag actttccccg ccgccagcag tgctttggca
    10501 tcggcaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt ggcatttcca
    10561 cctaacgccc ccagcgccgc cgcaatcggc agccccacca ttcccgtgcc gggaacggtg
    10621 acgcccagac cgttcttcat cagatttggc gaaacccagg cttctacacg ttcaaccgga
    10681 ccttccagtt ctgccgcagc aaccgccgcc gccagcgcca gtgaaatcgg ttcagtacat
    10741 cccagcgccg gttttacttc ctcctgaacg gcgaggatgt aacgctgcca taacggattt
    10801 aaagtcgaat caaacatatt aaaaacctta aaatttcagg taaatcaaga aaatgccagg
    10861 aacggagaaa cacaaagcaa caaaccagtg acgataatca ggtacagaga catccctttg
    10921 tatttgtgca atgccggtac tttgtaaacc agccacgccg ggatcaggca ccctaccatg
    10981 ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg gtgcgttcag tacgatagcg
    11041 ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct gaacaagatt ttcgttaatc
    11101 ttctcggcag gcatcttgcg acgcaggatg ttcattacga tcccttgcgt tgcttcgcga
    11161 aagcctaaat agacgccaaa gaatgcagtc atgacagcaa agatattgag aatgacgctg
    11221 accactttca cccacgctgc gccgtcaccg ctaataaact gtgcggcaat cgccagcgcg
    11281 gaaatattct gctcgtaggc ttttaccgct tcgtcatgcc ccatcgccag cgtgaacgac
    11341 acggcgtaga aaaagacggt gacaaacaaa atgccaaacg cgatattcat tgcccgcaat
    11401 gctttatgcc gcgccacttc aatagatttt tcccgcgagc gataagagat caccatcggg
    11461 cttaacgtct ggataaacaa aatcgacgtc agggtaaacg gcagcgtaat aatggcgttt
    11521 ttcaccagca gccccagcgg cggtagcgaa ccgacgttat acagatgcca cattcccacc
    11581 atcgacacgc ccagcgccgc gaccaccagc agcttggtca gcaccatgcc ggtcgaaatt
    11641 ttgaataaca atttctcgcc gcgtgaagag atcgccacca gaatgcaaat cagtaccaga
    11701 ccataaaagg gactgtctga cagcaaccct tccgtcacgc cgaaggtatg cagataggaa
    11761 gcactatcgt tggtgatggc ggtggaatag acgaacatcc aaatcaccag cattacgaaa
    11821 tagagcgcac ctaacaggat gccccagttt ttacctaaat aaccgctaat gacactcggg
    11881 taatctttac attctggtga ttctgccagc gtattaataa acaaccgctg aaacagatac
    11941 attgccgggt aaccaatcac cgatgagagc aaaaataccc acaatcccat caaaccgacc
    12001 tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc cgatactcat aatcacccag
    12061 ccggtgtcag tactgtcgaa tttgatcgct tctcgccact cgctctcact cattccagca
    12121 cgacccgccg gggtcgaagc gtctgcaatg acgcctttat tcgatgcgat ttccataatt
    12181 tctcgctcaa tattttgtag ggcttattat ttttttccga gccgcatcaa ggcgatatgc
    12241 ggtctacgtt tttgcaggcg agttattaga ataaagaaat gatacgcgcg aggtcagaga
    12301 aaatcttcac aatcaagact ttgaaataac ggagatggat aagaattttc tactaaatta
    12361 atcgcaagag aatattcatt ctctatttgc ggcagatcac aaaaaaaagg tgcacatttg
    12421 tgcacccaag gatgaaagct gacagcaatg tcagccgcag accactttaa tggccagtcc
    12481 tccgcgtgat gtttcgcggt atttatcgtt catatctttg ccggtttcat acatcgtctc
    12541 gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca tccacgcggc
    12601 gtttactgct ttcacggcat taatggcatt acgttcaatg cacgggattt gtacctgtcc
    12661 ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc gcgatttccg ccgcattgca
    12721 tacctgcgcc ggactgccgc ccagtagttc agttaaccct gccgccgcca ttgaacaggc
    12781 cacgccaatc tccccctgac agccgacctc cgcgccagag atggaggcgt tcattttata
    12841 cagcgcgcca atagcccccg ctgccagaaa atagcgggca attgaccgct cgtttaccgg
    12901 acgacggaac ttatcgtaat aagccagcac tgccggaata atgccgcacg caccgttagt
    12961 cggtgccgtt accacgcgcc cgccagctgc gttttcttca ctaaccgcca gcgcgtacat
    13021 gttgatccag tcgatgacat tcatcggatc gttagagatg ttatcgctag aaaccagctg
    13081 acgacgcagc gctacggcac gacgcggcac attgagcgga ccaggcagca cgccttcagt
    13141 gttcatcccg cgttcaatac cgtcatgcat cacttgccag atacgggcaa atcctgcgtc
    13201 aatttccgct ttgctgcgca gcgccagctc gttgtgcatc atcaggccag atatagacag
    13261 gccgttgtaa tcacacattt tcagcagttc acctgctgag tggaaatcgt aaggtacgga
    13321 cgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc cgccgccgac
    13381 agagtaatag gttttactta atagcgcttc ctgtcccttc caggcagtga tccgcattcc
    13441 gttctcatgg cgaggcaaca tttcgggatg gaagataatg ttctttgcta caggaaaatc
    13501 aacaatatgc gcaccagatg ccactggcag ccgcccgctg cgcgttacta actctataaa
    13561 tgcagggatc tcatcaatga caacatcctg cggactgttt cctgccagtc ccatgataat
    13621 ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac gacccataca gatcgaccac
    13681 aatatggctc gtcgcggtta ataagccgct actttccagc cgatcaataa aactttttcc
    13741 ggcattcatt ggccccacgg tatgcgaact ggagggacca atcccaattt tgaaaatatc
    13801 gaatgcacta atcatatcca caccctcgga ttgccgttca gtgaagtgga gcggaacgac
    13861 cttgcgaccg tcccgctcac gaggctttac gcactacgta ctgcgatagc ttcaatttcc
    13921 agcttcacat ctttcggcaa acgcgcaacc tgcacacagc tccgggtcgg gtaggtcgcc
    13981 tgatgctcat cgaagaactg cttatagact tcattgatgg tggcaaaatc attcagatcg
    14041 gtgatgaaca cggtcatctt gatgatatcg cccacgctca gcccggcagc aaccacgatc
    14101 gctttgacgt tttcgaggct taaacacgcc tgatcttgca catcagccgg gatctcaccg
    14161 gtctgcgggc aaaccggaat ttgcccggag gtgaagacca tgctgcctaa atcaacgccc
    14221 tgaacataag ggccgattgc gcctggggca cgttgcgttt cgataatctt tttcatacat
    14281 cctccggcgt cagagcgcct gggtaaaagt acgtgaaata acatcctgtt gctgttcacg
    14341 ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg attgtcaggt taggatattt
    14401 ttccgggtgc tcgatggcat ccagcagcat ttcccgattc attacgttga cgttgaggtg
    14461 ttgaccgcct tcgacatccg cttcgtggtg gaaataccca tccagcaggc cgacaaggtt
    14521 ggttttacgt actggatctt ctttgcccag cgccgcagga acgattgaga aggtgtacga
    14581 gatcccatct ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg aggccacggc
    14641 acctttgcgg tcacgaccat gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg
    14701 acgaccgtcc ggcgtattac cggttttctg gccgtacacc acgttagaag tgatagtcag
    14761 aatcgactgg gtagggacgg cgttgcgata ggttggcagc gctttaattt tcttcataaa
    14821 gcgttcaacc aggtcgcagg caatgctgtc tacgcgctcg tcgttgttgc cgtactgcgg
    14881 atattcaccg tcgatttcaa agtccaccgc caggccgttt tcgtcacgga ttggtttcac
    14941 gcgggcatat ttgatggcag acagggagtc cgtcgccacc gacaggcccg cgatgccgca
    15001 tgccatagtg cgatagacat cacgatcgtg cagcgccatc agcgaagctt cgtagctgta
    15061 cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg tactgcaccg ccagccagtc
    15121 catgaagtga tcgaggctgt ccatcacttt gtcgtaatcc agcacgtcgt ccatcagcgg
    15181 tgctgttttc ggcccgacct gaatcttcag cttctcgtcc accccgccgt taattgcgta
    15241 cagcagtgtt ttcgccaggt tagcgcgtgc accaaagaac tgcatttgct taccaatcac
    15301 cattgggctg acgcaacaag caattgcgta atcgtcgctg ttgaagtcag tacgcatcag
    15361 atcgtcgttt tcatactgca aggaagaggt gacgatcgac acctgcgcgg catatttttt
    15421 gaaggcaatc ggtaattctt ccgaccaaag aatggtcagg ttaggttccg gtgccggccc
    15481 catagtgtgc agggtgtgca aatagcggaa ggagtttttg gtcaccagcg tacgaccgtc
    15541 cagccccatc ccgccgatca cttccgtcgc ccagattggg tcgccggaga acagcgaatc
    15601 aaattccggc gtacgcaaga agcgcaccat acggatcttc ataatgaagt gatcgatcag
    15661 ttcctgtgcc tgctgctcat tgagtacgcc tgctttaaag tcgcgctcaa tgtagatatc
    15721 gaggaacgac gccgtgcgcc ccagcgacat cgcgccgccg ttctgtgatt tcactgccgc
    15781 cagataagcg aagtagagcc actgcaccgc ttcctgcgca ttctgcgccg ggcgagagat
    15841 atcaaagcca tatttcgccg ccatttcctg aatctgcaac agcgcatgac gatgctctgc
    15901 cagctcctca cgcagacgga tggtggcttc cagatcttcg cctttttcca gacgggactg
    15961 gagatcggca aattgcagtt cgcgttcacg caccagataa ctgatgccat acagcgctac
    16021 gcggcgatag tcaccgataa tgcgcccacg gccatagcca tctggtaaac cggtcagcac
    16081 gccagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc cctggttatg
    16141 ggttttacgc agatcggtaa acagatattc aaattcactg tccatttcac ggccataggc
    16201 gtggaatgaa cttttaatca tattaatgcc accgaacgga tgcagcgcgc gctttaatgg
    16261 cgcatccgtt tgcagaccaa caattttttc cagcggctgg ttaatatatc ccgcatcatg
    16321 agcggtaatt gtggtggcaa tattggtatc gaaatcaacc ggcgcgtggg ttgcgttttc
    16381 gatacggatg ccttccatta ctttttccca caattccgtg gtggcaggcg tcgcttcggc
    16441 gaggaaagat tcatcgcctt catacggtgt atagttatgt tgaataaaat cgcggacatt
    16501 aatttcgttt ttccagtccg tacctttaaa gccaagccat gcgtcggcgt acagcttatc
    16561 gctggtatca atatctatct tcatgaaaaa taatctctct acaatacttc aactaaatta
    16621 tgcaaattct gcgggcgcgt taactttgcc taaatgaatg gcatccaaag caatcatttt
    16681 ttcttcgtta gtcggaataa cggcacaaat gacacgcgca ttttcactgg aaacaattcg
    16741 ctctccaaaa gagttagagc gattattcat ttctgtatca atcactacgc ctaatacagc
    16801 caaatgttcc atgaccagac gacgaattaa acttgaattc tctcctattc cgccggtgaa
    16861 tataattcca tccaggcgac gtaatgaagc tgcgtgtccg gcaatatgac gggcaattcg
    16921 gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc
    16981 cagaacacgt aaatccgaag aaagaccgga aatacctaat aatcccgact ctttattcac
    17041 tacgcgttcc aggtcaccca ggctctggtt ggtttggctg gcgacccagg acatcgcacc
    17101 aaagtcgaca tcgccactgc gggttcccat catcaagcct tccagcggcg tcattcccat
    17161 tgaagtatca acactctgac cgttgcgaac cgcgcagatt gacgcgccat tgccaagatg
    17221 cgccacaacc aggccggagt catcttccgc cagattcagc agagaatgtg cgcgctggga
    17281 aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt acacctaact cttcataata
    17341 tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga aactggtatc
    17401 aaataccgcc acctgagtta cgcccggaaa taattgctgc gccgattcaa taccacttaa
    17461 attggcgtaa ttatgcaggg gtgccagtgg agaaacgcga cggatattat caatgacttc
    17521 atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc ggtggccaat
    17581 taaggccaca ctgtcattta aattccgttt ttccagttca aatgcaattg ccttcaatgc
    17641 accttcgtag ctgtggtgag ccagcggtgc tggctctccc ccatttacgg ataagaatgc
    17701 attttccgag ttaataccgt cggcaatacc tgacattaat acttcacagt cgctggcatc
    17761 gagcacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa ccggaaattc
    17821 attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg atgttcagga
    17881 tggtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg tatttcgcca
    17941 gagacggcgc tttacggatg gcatacatcg gcaacaggca cagcagggat gcgataatcg
    18001 gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca acaacccagg
    18061 tggagcccat gatgaagatc atgctgatag tgttcagttt acccagcgac actttagttt
    18121 tgtcgccttt ataaccaaac ttcaggacca ggccattcag accttccagc gttcccagat
    18181 agtgaccgaa gaaagatttg aagatagcca cgagtgcgat gatggaagcc gcatattcca
    18241 gtgtaatcgc gaacgttgtt ttggtaccgg tcatggacgc aaagtggtta gccagataag
    18301 aaagcactgg aatattctgc gctttggctt ccgccatgtt ggccggagac agcgtaaata
    18361 ggcagctaaa ggcaaagaac atcaccactg caaccatcag catgctggca cgagaaatga
    18421 tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac tcttcacgtt
    18481 tagaaaccac gaaggaagag acgattggcg agaaattaaa ggagaaaacc atgatggaaa
    18541 tccccagcca gacggtgatc aggataccgt catgaccggt taacgacagc gaaccgaggt
    18601 caacctggtc gataactgca gagttccagt aagggatcag cgacagagaa atcagcacca
    18661 ggctggcgat aaacggccat accaggtagc tcatcacttt aaccatcaga tccttaccaa
    18721 accagatgac gaaagccatc agcagcagca ggaacagcgc cacaaagccg cgattcagcg
    18781 gtgcaaagcc gagctggttt tcccagaacg tcataaaggt attggtaata gtaacgccat
    18841 aaatccacag cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc acgccgccag
    18901 ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta gagccagaaa
    18961 gacatagacg cgccagcgcc cggtggcaat aaaatgcgat ggggtatgcc aataccaaca
    19021 tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac agcaccccgg
    19081 cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta cgccaggacg
    19141 attgttttgt ctggctggat acaatgctat ctgaagtact catatcctat cctcaacgaa
    19201 ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg atattgccgc
    19261 cggaaataat actgacggtt tttctgtttt gaatatattg gtctaattta ccgcttaata
    19321 atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc tgaattaagg
    19381 caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg actaattcac
    19441 gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc gccagggtgc
    19501 cggtagttcg gtgcgtggtt atttctccgg agtggaaaga agccgccatg ccgtgaacgt
    19561 tttcagactg tacgccaata acacgaatgg tcgggttaat agatttaatt gccaccgcaa
    19621 taccagcaat taaaccgcca ccaccaattg gcacaatcac gttatcgaca tcatagagat
    19681 cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga tcatcgtaag
    19741 gtgggataaa aatacggcct tccatttcga caatttcgct cactttagcg atagtgtcgt
    19801 tgaagttatc accatgcaga acgacttctg cggagtagtc gcacgttgcc gctactttgg
    19861 attttggcgc accttttggc atcaccactt taccgtcgat acccagcatc gcgcaggaga
    19921 gcgaaacccc ttgcgcatgg ttgcccgcag aacaggccac cacgcctttg cgtttttccg
    19981 catcggtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa cccgtacgct
    20041 gcatattttc aaacttcagg aatatttcac ctttgcaacg ttcactaaaa tagttggagc
    20101 gaggcatgcc tgttttataa attcgcccag ccagtcgttg tttcgcttca ataatatcat
    20161 caatagcaat cggcagatcg tatgtaatat gcattataaa acctcttcgc ctgaattaaa
    20221 tatgtaggta accgtaaccg acacctgcaa aacagacagg tggattattt atgtttaaat
    20281 aaaaacaaat aactaaccaa cttctattaa ttgccttcgt ctacacccat tataagatga
    20341 atactctttg gctaattcca ccaaaaccga tgctgctttt ttaatacgat aatttttcga
    20401 ccataccgcg gcatattgtg ccacaggtaa tgtttcttca accggaatag taataaattg
    20461 attagaacca aaaggtgacg tcatatcaca aggaattaca gttaagaaat cagcattgag
    20521 aacaagatta taaattgtca cgactgagtc ggttttaacg atgttttcaa tactgatgcc
    20581 atttctttgt aacgtagtaa gcagttcgct gtagtacccc atattagttt gtggcaacac
    20641 ccactgttcg ttcttcaacg actccagcgt ggtggtgccg gtgcatgttc gggacttact
    20701 ggctaccagc acaaactcgg actcgaacag cggctcaaca tgtaaatcct gaagcttcat
    20761 ttctgcactt aatgtaccaa tcgcaaaatc cagtcgaccg tcgcggattg ccggtaagaa
    20821 cgaagacagt tgcgcttcat acatagaaac ctgcgctttc gggaacacct ctttgaactt
    20881 gttgatcatc cctgacataa aagtaaaacc aatcaatgaa ggaaaaccaa atgagacttc
    20941 caccaccgcc tcagaagaca taccgcttat ctcattaacc atatttttca tttcacgggt
    21001 aatggattcg gaacgggaga gtaacaattg accggcaggt gttaatgtta caccggtatt
    21061 tttccgcacc actaattcca caccaaaata atcttcaata tcgttaatga ttttactgac
    21121 ggccggttga gttaacccta attcttttgc agccgagccg atagagccac ttctaatgac
    21181 ttcctgaaag actaccaggt gctgcgtttt cggaagaaga atagtgctca taacgaccta
    21241 cgttaattac ctcattgacg gcatgaagtg tatcaaaatg aaatgaacag gatatgtgcg
    21301 accactcaca aattaacttt caatactttc cagggtatcg tcattacaat taaatacctt
    21361 taaatatcaa caagttaaag cataaaaatc agcataaacc ctgatttttg tcaaaaaaaa
    21421 tgacggggat aaccatattt tatggcgata acatcattcg ttatgtggtt aatacaaaaa
    21481 aggggctgag accatatttc aagcaattac cggataatta ccaggcaaaa tttgagttaa
    21541 atttaatgag caagttctca aattttatta taaataaacc attttcagcg ataaatactg
    21601 cggcacgtca cattttttca cgctatttgt tggagaacaa acatttattt tatcaatatt
    21661 ttaaaatttc gaatacaggt attgatcatc tcgaacaatt gattaacgtc aactttttct
    21721 cttctgacag gacgtcattt tgtgaatgca atcgttttcc ataaattctt ctcccctcat
    21781 aggcgacgaa tagcattttg tgttgaggat cacaaaacga ataattgctg atcgccgcga
    21841 taaggtcaga caaagacaac aagggaaatt ttcacagagc ttttgatcgt cgtaggccac
    21901 agaatgttgc ttcgtttact gtaacgccgg gtaaatgagc gttttttgat agtgcgaaag
    21961 aaccctgcga ggaaaataag catctattat tgtttgtcat gacgacaagg caggcatcaa
    22021 tgaaaggctt cccaattgca catatttttc acccttcaat cccgccaatg cacgcagtgg
    22081 ttaacaatca caatagaaat attgattatt ggacggtaaa aagaaagttt gcagaaatt
//
LOCUS       DAETQN010000066        21995 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.066, whole genome shotgun sequence.
ACCESSION   DAETQN010000066 DAETQN010000000
VERSION     DAETQN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.066"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..986)
                     /gene="cmtA"
                     /locus_tag="K0W68_002516"
     CDS             complement(<1..986)
                     /gene="cmtA"
                     /locus_tag="K0W68_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBI3074047.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1014..1457)
                     /gene="cmtB"
                     /locus_tag="K0W68_002517"
     CDS             complement(1014..1457)
                     /gene="cmtB"
                     /locus_tag="K0W68_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HBI3074048.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1771..3762)
                     /gene="tkt"
                     /locus_tag="K0W68_002518"
     CDS             complement(1771..3762)
                     /gene="tkt"
                     /locus_tag="K0W68_002518"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBI3074049.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4040..4798
                     /gene="loiP"
                     /locus_tag="K0W68_002519"
                     /gene_synonym="yggG"
     CDS             4040..4798
                     /gene="loiP"
                     /locus_tag="K0W68_002519"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBI3074050.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4941..5861)
                     /gene="speB"
                     /locus_tag="K0W68_002520"
     CDS             complement(4941..5861)
                     /gene="speB"
                     /locus_tag="K0W68_002520"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBI3074051.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5999..7975)
                     /gene="speA"
                     /locus_tag="K0W68_002521"
     CDS             complement(5999..7975)
                     /gene="speA"
                     /locus_tag="K0W68_002521"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBI3074052.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7984..8115)
                     /gene="yqgB"
                     /locus_tag="K0W68_002522"
     CDS             complement(7984..8115)
                     /gene="yqgB"
                     /locus_tag="K0W68_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBI3074053.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8251..8466
                     /locus_tag="K0W68_002523"
     CDS             8251..8466
                     /locus_tag="K0W68_002523"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074054.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8770..9924
                     /gene="metK"
                     /locus_tag="K0W68_002524"
     CDS             8770..9924
                     /gene="metK"
                     /locus_tag="K0W68_002524"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBI3074055.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10360..11754
                     /gene="galP"
                     /locus_tag="K0W68_002525"
     CDS             10360..11754
                     /gene="galP"
                     /locus_tag="K0W68_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBI3074056.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11831..12328
                     /locus_tag="K0W68_002526"
     CDS             11831..12328
                     /locus_tag="K0W68_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBI3074057.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12423..13130
                     /gene="endA"
                     /locus_tag="K0W68_002527"
     CDS             12423..13130
                     /gene="endA"
                     /locus_tag="K0W68_002527"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBI3074058.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13210..13941
                     /gene="rsmE"
                     /locus_tag="K0W68_002528"
     CDS             13210..13941
                     /gene="rsmE"
                     /locus_tag="K0W68_002528"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBI3074059.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13954..14904
                     /gene="gshB"
                     /locus_tag="K0W68_002529"
     CDS             13954..14904
                     /gene="gshB"
                     /locus_tag="K0W68_002529"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBI3074060.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15013..15576
                     /locus_tag="K0W68_002530"
     CDS             15013..15576
                     /locus_tag="K0W68_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBI3074061.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15576..15992
                     /gene="ruvX"
                     /locus_tag="K0W68_002531"
     CDS             15576..15992
                     /gene="ruvX"
                     /locus_tag="K0W68_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBI3074062.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16167..17147)
                     /locus_tag="K0W68_002532"
     CDS             complement(16167..17147)
                     /locus_tag="K0W68_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBI3074063.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17165..17869
                     /gene="yggS"
                     /locus_tag="K0W68_002533"
     CDS             17165..17869
                     /gene="yggS"
                     /locus_tag="K0W68_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBI3074064.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17887..18453
                     /gene="yggT"
                     /locus_tag="K0W68_002534"
     CDS             17887..18453
                     /gene="yggT"
                     /locus_tag="K0W68_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBI3074065.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18450..18740
                     /locus_tag="K0W68_002535"
     CDS             18450..18740
                     /locus_tag="K0W68_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBI3074066.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIVALIN"
     gene            18748..19341
                     /locus_tag="K0W68_002536"
     CDS             18748..19341
                     /locus_tag="K0W68_002536"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBI3074067.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19334..20470
                     /gene="hemW"
                     /locus_tag="K0W68_002537"
     CDS             19334..20470
                     /gene="hemW"
                     /locus_tag="K0W68_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBI3074068.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20781..21767
                     /locus_tag="K0W68_002538"
     CDS             20781..21767
                     /locus_tag="K0W68_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBI3074069.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            21812..>21995
                     /locus_tag="K0W68_002539"
     CDS             21812..>21995
                     /locus_tag="K0W68_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HBI3074070.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLG"
BASE COUNT         5442 a         5715 c         5597 g         5241 t
ORIGIN      
        1 gcaaaggaca ccagggtagc taccgtaaca ccggcaattg tcgccaggaa cgagcctttc
       61 ggcgtcagtg ccaggtaagc aaagatagac cccggacttg ggccagccac cagaccaccg
      121 tccagtaagt taaacaccca ggtgccagac ataccgcccg caatcatggc aataatggtc
      181 agcggcttca tcagcacata cgggaaatac agttcgtgga tcccaccgag gaagtgaata
      241 atcatcgccc cgggcgcaga acgtttactc atccctttac caaacaaggt aaacgccagc
      301 agcagcccta agccaggccc aggattggac gccaccataa agaagatgga tttaccgtta
      361 accgaagcct gttgcattcc cagcggataa tagacgccct gatcgatcgc attattgagg
      421 aaaagcactt tcgccggttc attgatgact gacagcagag gcagataacc cgcatgtacc
      481 agcgcctcaa tacactcttt gacgagagta ttggcaatta acaccgccgg gccgataact
      541 tcaaaaccca gcagacaaag gagcatcccc gcgataccta atgagaagtt attgataacc
      601 atctcaaaac cggcaggtat gcgtttttcc agtgccttat caacatattt tatgaccagc
      661 ccaccgagcg gccccataat cattgagcca aggaacatcg ggatctctgc gccgacgatc
      721 acaccaatag tacctattcc gcccatgacc gcgccgcgtt taccgccgac cagatgacca
      781 ccggtagaac caatcatcac gggcaataaa taggtaatca tcgggccgac aattttggcg
      841 aaatgttcgt taggcagcca accggtggga ataaataacg cagtaataaa accccaggca
      901 ataaaagcac caatattggg gatgaccatt gccgtcaaaa agcccccaaa agcctggacc
      961 tttgcacgag cagacttgtt ttccataata ttatcctgta gaggagagag taattatccg
     1021 cggctgatta tgtccgctaa ttgtttttct gatgctgctg ttaagagttg ttcgagtatt
     1081 tcttcttcgc acagtaattc acttaacgcc tgaatagcgc caatgtgcga atcggcatcg
     1141 gcagcagaaa ggccgatgag taattttatt ggttcgtcat tccccggaaa ataaacacct
     1201 tgttcaagta atgtcaaaga catcccggtt ttaagcgccc cacattccgg tcgcgcatga
     1261 ggcattgcca cgcctggtgc gagaatataa taagggccat tgttaatggt ggaatcttta
     1321 attgcctgaa tgtaattctc gctgatatag tttttatcca gcaatgatac catcgagaaa
     1381 tcgatagctt cctgccaatc ttttgctgaa tgtataactg agattgatga ttctggaaaa
     1441 tagtcactaa gccgcataaa ttatccttat tttattttta gggtaccgtc cggcggtaaa
     1501 aataaaatac tcgtgcggtt taatgaaagc aattgagcag ggaaaccggt gttttgaaaa
     1561 cagtgaatta ttaatttctt atataacatt gagttataga tataacaaca aatgtcaata
     1621 cgcatatcgt gatgcatatt acataattga tatttattgc atataaatat ttgtgatcta
     1681 caacacgctt tatctattgc ttgtccgcaa acggacatat caaggtaata aaaaaggtcg
     1741 ccgaagcgac ctttttaccc gaaatgctaa ttacagcagt tcttttgctt tcgcaacgac
     1801 gttgtcgacg gtgaagccga actcttcgaa cagcaactct gccggagctg actcaccgaa
     1861 ggtggtcata ccgatgatag cgccattcag gccaacatac ttgtaccagt agtcagcaat
     1921 acccgcttct accgcaacgc gtgcagtaac cgctttcggc agtacggatt cacggtaagc
     1981 cgcatcctgc ttgtcgaatg catcggtaga cggcatggaa accacgcgcg ccttcacgcc
     2041 ttcggcagtc agtttttcgt aggcggcaac agccagttca acttccgaac cggtcgcgat
     2101 gaagatcagt tccggctgac cagcacagtc tttcagcaca taaccaccgc gcgcgatgtt
     2161 tgccagttgc tcttcagtac gttcctgctg cgccagattc tgacgggaga ggataagcgc
     2221 ggtcggaccg tcctgacgct caacaccgta tttccacgcg accgcggatt caacctggtc
     2281 acacggacgc catgtagaca tgttcggggt cacgcgcaga gaagcgacct gctcaaccgg
     2341 ctggtgagtc gggccgtctt cgcccagacc gatggagtcg tgggtgtaaa ccatcacctg
     2401 acgctgtttc atcagcgcag ccatacgtac agcgttacgt gcgtattcga caaacatcag
     2461 gaaggtggag gtgtacggca ggaagccacc gtgcagggag ataccgttag caatcgcggt
     2521 cataccgaac tcgcgaacac cgtagtggat gtagttaccc gcagcatctt cgttgattgc
     2581 tttagaacca gaccacaggg tcaggttaga cggtgccagg tcagcggagc cgccgaggaa
     2641 ttccggcaac agcgggccga acgcttcaat agcattctga gacgctttac ggctggcgat
     2701 tttcgccgga ttagcctgca gtttagcgat aaactctttc gctttggcgt cgaagtcaga
     2761 cggcatttcg cctttcatac ggcgggtaaa ttcagccgct tcctgcggat aagctttcgc
     2821 gtaagccgca aacttctcat tccatgcaga ttctttcgcc tggcctgctt ctttcgcatc
     2881 ccactgcgca tagatttcag acgggatttc gaacggtgcg tatttccagc ccagctgttc
     2941 gcgggtcaga gcgatttctg catcacccag cggcgcaccg tgggagtcgt gagtaccggc
     3001 tttgttcggg gaaccgaaac cgatgatggt tttgcacatc agcagggacg gtttgtcagt
     3061 cactgcgcgc gcttcttcta ctgcgcgttt gatggatgct gcgtcatgac cgtcgatgtc
     3121 gcgaataacg tgccagccgt aagcttcgaa acgcattgcg gtgtcgtcgg tgaaccagcc
     3181 ttcaacgtga ccatcaatag agataccgtt gtcatcgtag aacgcaatca gtttacccag
     3241 cttcagcgta cccgccagag agcaaacttc gtgggagatg ccttccatca tgcagccgtc
     3301 gcccatgaag gcgtaggtgt agtggtcgac aatgtcgtgg cccggacggt taaactgcgc
     3361 cgccagcgtt ttttctgcaa tcgccatacc gactgcgttg gcaataccct gacccagcgg
     3421 accggtggtg gtttccacac cagcggtata acctacttcc gggtggcccg gagttttaga
     3481 gtgcagctga cggaagtttt tcagttcttc catcggcaga tcgtaaccgg tgaggtgcag
     3541 caggctgtag atcagcatgg agccgtggcc gttggacagc acgaagcggt cacggtcagc
     3601 ccaggacgga ttctgcgggt tgtgtttcag gaaatcacgc cacaggactt cggcaatgtc
     3661 agccataccc ataggggcac ccgggtgacc ggatttggct ttctgtactg cgtccatgct
     3721 cagcgcacga atagcattgg caagctcttt acgtgaggac attttgactc cagatcggat
     3781 gatgaagggc acgcccttaa cgacttgacg acagcgcgtt ttgggctacg ccggaaaatt
     3841 tgccaacaat ttaccgcaag ccgcgcgtca tgtacatgga acatcctttt gccgcttcag
     3901 aaatctctgg atcatgctcg catgttgcgc aatctactcg cccgtccgct gcgcttttcc
     3961 ttatactgag actgagcgtc gattcacctg caaacggcgc atttttagaa taatcctgac
     4021 cttgtgcgga agagaaaaca tgaaaattcg cgccttattg gtagcaatga gcgtggcaac
     4081 ggtactgact ggttgccaga atatggactc caacggactg ctctcatcag gagcggaagc
     4141 ttttcaggct tacagtttga gtgatgcgca ggtgaaaacc ctgagcgatc aggcatgtca
     4201 ggagatggac agcaaggcga cgattgcgcc agccaatagc gaatacgcta aacgtctgac
     4261 aactattgcc aatgcgctag gcaacaatat caacggtcag ccggtgaatt acaaagtgta
     4321 tatggcgaag gatgtgaacg cctttgcaat ggctaacggc tgtatccgcg tctatagcgg
     4381 gctgatggat atgatgacag ataacgaagt cgaagcggtg atcggtcacg agatggggca
     4441 cgtggcgtta ggccatgtga agaaagggat gcaggtggcg cttggtacaa atgccgtgcg
     4501 agtagctgcg gcctctgcgg gcgggattgt cggcagttta tctcaatcac aacttggtga
     4561 tctgggcgag aaattagtca attcgcaatt ctcccagcgc caggaagcag aagccgatga
     4621 ttattcttac gatcttctgc gccaacgcgg catcagcccg gcaggtcttg ccaccagctt
     4681 tgaaaaactg gcgaaactgg aagaaggccg ccaaagctca atgtttgacg accatccggc
     4741 atccgccgaa cgcgcccagc atattcgcga tcgcatgagc gcggatggga ttaagtaaag
     4801 cctggtggtg tcgaacgtag gtcagataag gcgttcacgc cgcatctgac atcaatggca
     4861 catttgttac cctgtgcgca ttgccggatg cgatgctgac tgcatcttat ccggcctacg
     4921 ggtggcatcg catctggtgc ttactcgccc tttttcgccg cttggatata cagcatctcc
     4981 agcgccagcg tcgccgcagc cagagcggtg atttccgact gatcgtatgc cggagccact
     5041 tccactacgt ccatcccaac aatgttgaga tctttcaggc cgcgtaccag tttaatagcg
     5101 cgatcggagg tcaggccgcc aatcactggc gtaccggtgc ctggtgcaaa agcaggatcc
     5161 aggcagtcga tatcaaaagt caggtaaacc ggcatatcac ccacaatctg tttcacttgg
     5221 gcgataacgt catccacgct gcgatcgttc acctggcagg cgtccagcac ggtaaagccg
     5281 ttgtctttat caaactcggt acgaatacca atctgcacgg aatgattcgg gtcgatcaga
     5341 ccttctttcg gcgcggtata gaacatagtg ccgtggtcaa attcacaacc gttcgcatag
     5401 gtatcggtgt gggcgtcaaa gtgtaccagc gccattttgc cgaaatgctt cgcatgagca
     5461 cgcagcagcg gcagcgtaac aaagtggtca ccaccgaagg tgagcatacg cttaccggca
     5521 gccagcagct tctcggcgtg cgcctgcagc ttttcgctca tctcacgggc atcgccaaag
     5581 gcatatacca gatcgccgca gtccacgacg ttcagacgct cacgcatgtc gaaattccac
     5641 gggaagcggt tgtgttccca ggccagattc gtcgaaacct gacggatcgc tgccggaccg
     5701 tggcgaccac ccgcacgacc agaagtggcc atatcgaacg gcacgccagt aatcacccag
     5761 tctgcatcgc tgtcatacgg ctggaagttc atcggcaggc gtaaaaaacc aaaggcattg
     5821 gaaaccagtg agttatcgta ttgatgacct aaggtgctca tggatggact cctattacaa
     5881 agtcgatata aaaaaaccct tccgcgtcgt taggcccgac gaggaagggt tggatttgtc
     5941 acaataaatt gtggcggatt atcaccgcta aattaagcgg attcaagtaa cacaggactt
     6001 actcatcttc aagataagta taaccgtaca aacctgcctc gaactcttca aggaactgtt
     6061 gttgcagttc agcatcaaga tcggttttct tcacttgatc gcggaactgg gttaacagcg
     6121 ttttcggatc gagctgtaca tattgcagca tgtccgccac ggtatcgcct tcgtcagaca
     6181 gttctacttc tacgctaccg tcagggaaca cgaacacgtc aaccgcttcg gtatcaccga
     6241 acaggttgtg catattgcca aggatctcct gatatgcgcc gaccataaag aaaccgagca
     6301 tcggcggatt ctctggatcg tactccggca ttggcattgt cgtggcaata ccatcaccat
     6361 caatatagtg gtcgatagca ccgtcagagt cacaggtaat atccagcagc acagcgcgac
     6421 gttccggcac ttgatccagc ccttccagcg gcagaaccgg gaacaactgg tcgatccccc
     6481 atgcgtccgg cattgactgg aacagcgaga aattgacgta cattttgtcc gccatacgtt
     6541 cctgcagttc gtcgattatc ggacgatgcg cacggttttg cggatccagt tgcttctgca
     6601 cttcatggca catgctcaga tagagttgtt ctgcccatgc acgttcttgc aggctaaagg
     6661 tgccggaaga gtagccgata tgaatgtcgt gcagatccat ctggctgtcg tgtaaccatt
     6721 cacgcaaaga acggcgagtt cccggttcgt gcatttcctg ccaggtttcc cacatgcttt
     6781 gcagcgcgcg cggcgcatct tctgcgggcg cggtcggcac cgtatattcg ttacgttcca
     6841 cgccgatgat attcgacacc agcacggtat ggtgcgcagt caccgcacgg cccgattcgg
     6901 tgattaccgt cggatgcggc agaccatttt cttcacacgc atcgccaatc gcccagatga
     6961 tgttgttggc gtattcattg aggccatagt ttacggagca gtcagactgc gaacgggtgc
     7021 cttcatagtc cacgcccaga ccgccgccga cgtcgaagca ctgaatattg acgcccagct
     7081 tgtgcagttc cacatagaaa cgcgcggatt cacgaacgcc cgtggcgata tcgcgaatat
     7141 tcgccatctg cgaaccgagg tggaagtgca gtagttgcag gctgtcgaga cgcccggctt
     7201 cacgcagggt ttcaaccagt tgcagtacct gagtcgcagc caggccgaac ttcgattttt
     7261 ccccgccgga ggactgccat ttacccgagc cctgcgaagc cagacgtgca cgcacgccca
     7321 gacgaggaac gacattcaga cgttctgctt catccagcac aatggcgatt tctgacatct
     7381 tctcaatgac cagatagacc ttgtgcccca tcttctcgcc aattaatgcc aggcggatat
     7441 attcgcggtc tttataaccg ttgcagacga tgacgctacg ggtcatgcca gcatgtgcca
     7501 gtactgccat caactcggct ttggaaccgg cttccagacc cagcggttcg cccgaatgaa
     7561 tcagggactc aatcacgcgg cggtgctggt taactttgat cggataaaca aggaagtaat
     7621 cgccgttata gccgtaggat tccctcgcac gtttgaacgc ggcgttaatg gaacgcaaac
     7681 ggtgctgcag gatctgtggg aaacagaaca gtgcaggcag acgctgaccc tgtgcttcac
     7741 gagttttcac taactgcgcg agatcgacgc gagcttccgg gacgtccggg tccgggcaca
     7801 cgctgatgtg gcccagctcg ttaacgtcat agtagttatt gccccaccag gcaatattgt
     7861 aagtacgcag catcttgctg gcttcctggg agctcattgc aacctcctgc atggagcgta
     7921 gtacaccgtg ttcgcccgct gacgaaggca aacccataga catgtcgtca gacatagcga
     7981 acctcaaatt attttattaa gtgtaaaaca gttaacgact atcgcagcct ggaactgcga
     8041 taacaaccca taagcacatg gattttccag cagtgaatgc tgacgctcca actgcgcaac
     8101 cggtttcttt ttcataacat tattaagcac ataaccgaac gtaagtgtga cagttcggcg
     8161 aaaccacgag aaaactcttg tttttacaag agcgcccttg ttcagtcctc agtaactgta
     8221 accagctctt gaatcctgag aagcgccgag atgggtataa catcggcagg tatgcaaagc
     8281 agagatgcag agtgcgggga acgaatcttc accagaacgg tgagacaggt taagcagcag
     8341 acaacggttc attatttcgt atcacctcca cggccgcctg ttaagacgaa cccacaagcc
     8401 aaaactctga tttcaacccg gctggaagtg gcaacacgaa agaaacgtcg tgtgcttttt
     8461 atttaagccg cgcgccgcgt tttatacccc acaatggcag aaaattgcaa aagataaata
     8521 cgcagaatgc cggcattgtc aggaaaaatt tccagccacg tttttaacag aatgagacac
     8581 gattcaaaaa aaagtggaaa tcgggtgaag aattgaccta aaatagccat ccagatgtta
     8641 atccatccat accgattaac actcagactg ccagtgtttt taacctgcag agtcgtggta
     8701 ggatccgcta ccacagaaaa tccacacaac agtttgagct aaccaaattc tctttaggtg
     8761 atattaaata tggcaaaaca cctttttacg tccgagtccg tctctgaagg gcatcctgac
     8821 aaaattgctg accaaatctc tgatgccgtt ttagacgcga tcctcgaaca ggatccgaaa
     8881 gcacgcgttg cttgcgaaac ctacgtaaaa accggcatgg ttttagttgg cggcgaaatc
     8941 accaccagcg cctgggtaga catcgaagag atcacccgta acaccgttcg cgaaattggc
     9001 tatgtgcatt ccgacatggg ctttgacgct aactcctgtg cggttctgag cgctatcggc
     9061 aaacagtctc ctgacatcaa ccagggcgtt gaccgtgccg atccgctgga acagggcgcg
     9121 ggtgaccagg gtctgatgtt tggctacgca actaatgaaa ccgacgtgct gatgccagca
     9181 cctatcacct atgcacaccg tctggtacag cgtcaggctg aagtgcgtaa aaacggcact
     9241 ctgccgtggc tgcgcccgga cgcgaaaagc caggtgactt tccagtatga cgacggcaaa
     9301 atcgttggta tcgatgctgt cgtgctttcc actcagcact ctgaagagat cgaccagaaa
     9361 tcgctgcaag aagcggtaat ggaagagatc atcaagccga ttctgcccgc tgaatggctg
     9421 acttctgcca ccaaattctt catcaacccg accggtcgtt ttgttatcgg tggcccgatg
     9481 ggtgactgcg gtctgactgg tcgtaaaatt atcgttgata cctacggcgg catggcacgt
     9541 cacggtggcg gagcattctc tggtaaagat ccatcaaaag tggaccgttc cgcagcctac
     9601 gcagcacgtt atgtcgcgaa aaacatcgtt gctgctggcc tggccgatcg ttgtgaaatt
     9661 caggtttcct acgcaatcgg cgtggctgaa ccgacctcca ttatggtaga aactttcggt
     9721 actgagaaag tgccttctga acaactgact ctgctggtac gtgagttctt cgacctgcgc
     9781 ccatacggtc tgattcagat gctggatctg ctgcacccga tctacaaaga aaccgcagca
     9841 tacggtcact ttggtcgtga acatttcccg tgggaaaaaa ccgacaaagc gcagctgctg
     9901 cgcgatgctg ccggtctgaa gtaatctttc ttcacctgct ttcaaaggcc agcctcgcgc
     9961 tggccttttt cttttacctc accgcggtgg ataggcattc acgccgcatc cggcaaaaag
    10021 ctgcccgcac aataacatca ttcttcctca acacgtttta tcgcaaatta tcatcactac
    10081 tgaaaccgat tacaccaata acaccagcca aatatttgta atattttcat attattattc
    10141 agttttcaca gttgttacat ttcttttcag taaagtctta attgcagata acagcgttta
    10201 atctatgatg atataactca attattttca tgcacttaaa ccataactaa gataaatgtt
    10261 agtgtaagcg attacactga tgtgatttgc ttcacatctt tttacgtcgt actcacctat
    10321 cttaattcac aataaaaaat aaccatattg gagggcatca tgcctgacgc taaaaaacag
    10381 gggcggtcaa acaaggcaat gacgtttttc gtctgcttcc ttgccgctct ggcgggatta
    10441 ctctttggcc tggatatcgg tgtaattgct ggcgcactgc cgtttattgc agatgaattc
    10501 cagattactt cgcacacgca agaatgggtc gtaagctcca tgatgttcgg tgcggcagtc
    10561 ggtgcggtgg gcagcggctg gctctccttt aaactcgggc gcaaaaagag cctgatgatc
    10621 ggcgcaattt tgtttgttgc cggttcgctg ttctctgcgg ctgcgccaaa cgttgaagta
    10681 ctgattcttt cccgcgttct actggggctg gcggtgggtg tggcctctta taccgcaccg
    10741 ctgtacctct ctgaaattgc gccggaaaaa attcgtggca gtatgatctc gatgtatcag
    10801 ttgatgatca ctatcgggat cctcggtgct tatctttctg ataccgcctt cagctacacc
    10861 ggtgcatggc gctggatgct gggtgtgatt atcatcccgg caattttgct gctgattggt
    10921 gtcttcttcc tgccagacag cccacgttgg tttgccgcca aacgccgttt tgttgatgcc
    10981 gaacgcgtgc tgctacgcct gcgtgacacc agcgcggaag cgaaacgcga actggatgaa
    11041 atccgtgaaa gtttgcaggt taaacagagt ggctgggcgc tgtttaaaga gaacagcaac
    11101 ttccgccgcg cggtgttcct tggcgtactg ttgcaggtaa tgcagcaatt caccgggatg
    11161 aacgtcatca tgtattacgc gccgaaaatc ttcgaactgg cgggttatac caacaccaca
    11221 gagcaaatgt gggggacagt gattgtcggc ctgaccaacg tacttgccac ctttatcgca
    11281 atcggccttg ttgaccgctg gggacgaaaa ccaacgctaa cgctgggctt cctggtgatg
    11341 gctgctggca tgggcgtact cggtacaatg atgcatatcg gtatccactc tccgtcggcg
    11401 cagtatttcg ccatcgccat gctgctgatg tttattgtcg gttttgccat gagtgccggt
    11461 ccgctgattt gggtactgtg ctccgaaatt cagccgctga aaggccgcga ttttggcatc
    11521 acctgctcca ctgccaccaa ctggattgcc aacatgatcg ttggcgcaac gttcctgacc
    11581 atgctcaaca cgctgggtaa cgccaacacc ttctgggtgt atgcggctct gaacgtactg
    11641 tttatcctgc tgacattgtg gctggtaccg gaaaccaaac acgtttcgct ggaacatatt
    11701 gaacgtaacc tgatgaaagg tcgtaaactg cgcgaaatcg gcgctcacga ttaatctcct
    11761 caggcttcct cccgtcgcgg aggaagccgc ctcttgcagt catcttttct tcgctctatc
    11821 ctctgccgct atgaaaacat cccgtctccc tatcgccatc caacaggccg ttatgcgcgg
    11881 cctgcgggaa aaactcgccc aggccaacct gaagctaggg cgtaactacc cggagccaaa
    11941 actctcttac acccagcgcg gaacctccgc cggaacagcc tggctggaaa gctatgaaat
    12001 ccgcctcaat cccgttttgc tgttggaaaa cagtgaagct tttattgaag aagtggtacc
    12061 gcacgaactg gcacatttgc tggtatggaa acatttcggc cgcgtagcgc cacatggcaa
    12121 agagtggaag tggatgatgg aaagcgtgct gggtgttccc gcccgtcgta cgcatcagtt
    12181 cgaactgcaa tccgtgcgtc gcaacacctt cccctaccgc tgcaagtgcc aggagcatca
    12241 gcttaccgta cgccgccata atcgcgtagt tcgtggcgag gccgtctatc gctgtgttca
    12301 ctgcggtgaa cagctggttg cgaaataacc atctgaacta tcaggaactt tcctgatctg
    12361 gctgattgca taccaaaaca actttcgcta cgttgctggc tcgttttaac acggagtaag
    12421 tgatgtaccg ttatttgtct attgctgcgg tggtactgag cgcagcattt tccggcccgg
    12481 cgttggccga aggtatcaat agtttttctc aggcgaaagc cgcggcggta aaagtccacg
    12541 ctgacgcgcc cggtacgttt tattgcggat gtaaaattaa ctggcagggc aaaaaaggcg
    12601 ttgttgatct gcaatcgtgc ggctatcagg tgcgcaaaaa tgaaaaccga gccagccgcg
    12661 tagagtggga acacgtcgtt cccgcctggc agttcggtca ccagcgccag tgctggcagg
    12721 acggtggacg taaaaactgc gctaaagatc cggtctatcg caagatggaa agcgatatgt
    12781 ataacctgca gccgtcagtc ggtgaggtga atggcgatcg cggcaacttt atgtacagcc
    12841 agtggaatgg cggcgaaggc cagtacggtc aatgcgccat gaaggtcgat ttcaaagaaa
    12901 aagctgccga accaccagcg cgtgcacgcg gtgccattgc gcgcacctac ttctatatgc
    12961 gcgaccaata caacctgaca ctctctcgcc agcaaacgca gctgttcaac gcatggaaca
    13021 agatgtatcc ggttaccgac tgggagtgcg agcgcgatga acgcatcgcg aaggtgcaag
    13081 gcaatcataa cccgtatgtg caacgcgctt gccaggcgcg aaagagctaa cctacactag
    13141 cgggattctt tttgttaacc cctaccccac gcgtacaacc gcgtggggag acgacgcgga
    13201 tttttaacta tgcgtatccc ccgcatttat catcctgaac cactgaccag ccattctcac
    13261 atcgcgcttt gcgaagatgc cgccaaccat atcgggcgcg tactgcgcat ggggccgggg
    13321 caggcgttgc aattgtttga cggtagcaac caggtctttg acgccgaaat taccagcgcc
    13381 agcaaaaaaa gcgtggaagt gaaggtgctg gaaggcaaga tcgacgatcg cgaatctccg
    13441 ctgcatattc acctcggtca ggtgatgtcg cgtggtgaaa aaatggaatt tacgatccag
    13501 aaatcgatcg aactcggtgt aagcctcatt acgccacttt tttctgagcg ctgcggcgtt
    13561 aaactggata gtgaacgtct gaacaagaag ctccagcagt ggcagaagat tgcaattgct
    13621 gcctgtgagc agtgtggtcg taaccgggtg ccggaaatcc gtccagcgat ggatctggaa
    13681 gcctggtgtg cagagcagga tgaaggactg aaactgaatc ttcacccgcg cgccagtaac
    13741 agcatcaata cgttgccgtt accggttgaa cgcgtccgcc tgctgattgg cccggaaggc
    13801 ggtttatcgg cagatgaaat tgccatgact gcccgctatc aatttactga tatcctgttg
    13861 ggacctcgcg ttttgcgtac agagacaact gcgctcaccg ccattaccgc gctacaagta
    13921 cgatttggcg atttgggcta acggagaaga ataatgatca agctcggcat cgtgatggac
    13981 cccatcgcaa acatcaacat caagaaagat tccagtttcg ctatgttgct ggaagcacag
    14041 cgtcgtggtt acgaacttca ctatatggag atgggcgatc tgtatctgat caatggcgaa
    14101 gcccgcgccc atacccgcac gctgaacgtg aagcagaact acgaagagtg gttttcgttc
    14161 gtcggtgaac aggatctgcc gctggccgat ctcgatgtga tcctgatgcg taaagacccg
    14221 ccgtttgata ccgagtttat ctacgcgacc tatattctgg aacgtgccga agagaaaggg
    14281 acgctgatcg ttaacaagcc gcagagcctg cgcgactgta acgagaaact gtttaccgcc
    14341 tggttctctg acttaacgcc agaaacgctg gttacgcgca ataaagcgca gctaaaagcg
    14401 ttctgggaga aacacagcga catcattctt aagccgctgg acggtatggg cggcgcgtcg
    14461 attttccgcg tgaaagaagg cgatccaaac ctcggcgtga ttgccgaaac cctgactgag
    14521 aatggcactc gctactgcat ggcacaaaat tacctgccag ccattaaaga tggcgacaaa
    14581 cgcgtgctgg tggtggatgg cgagccggta ccgtactgcc tggcgcgtat tccgcagggg
    14641 ggcgaaaccc gtggcaatct ggctgccggt ggtcgcggtg aacctcgtcc gctgacggaa
    14701 agtgactgga aaatcgcccg tcagatcggg ccgacgctga aagaaaaagg gctgattttt
    14761 gttggtctgg atatcatcgg cgaccgtctg actgaaatta acgtcaccag cccaacctgt
    14821 attcgtgaga ttgaagcaga gtttccggtg tcgatcaccg gaatgttaat ggatgccatc
    14881 gaagcacgtt tacagcagca gtaacccacc ttagcgagaa ggatctcgtt gagactctga
    14941 gtgacagcgc ccttctttcc acgcatactg ggcgctgttg cttttttgaa ccaggaaaca
    15001 gaacctctga caatgaattt acagcatcac tttcttattg ccatgcctgc tctccaggat
    15061 ccgattttcc gtcgttccgt ggtctacatt tgcgaacata ataccaatgg tgcaatgggg
    15121 atcatcgtca acaagccgct ggaaaatctc aaaattgaag ggattctgga aaagctgaag
    15181 atcacgccgg agccgcgtga tgaatcaatc cgtctggata aaccggttat gctcggcggt
    15241 ccgctggctg aagatcgcgg gtttattttg catactccgc cctccaattt tgcttccagc
    15301 attcgcattt cagacaacac agtgatgacc acttcccgcg atgtgctgga aacgctcggc
    15361 accgataaac aaccgtctga cgtattggtg gctctaggtt atgcctcctg ggaaaaaggt
    15421 caactggaac aagaaattct cgataacgcg tggctaacgg ccccggcgga tctgaatatt
    15481 ctgttcaaaa cgccgattgc cgaccgctgg cgcgaggcgg caaaactgat tggtgtggat
    15541 attctcacca tgcctggtgt ggcagggcac gcctgatgag tggaacctta ctcgccttcg
    15601 acttcggcac caaaagcatt ggcgtagcgg tcggccaacg cattaccggc actgctcgcc
    15661 ctttgcctgc aattaaagca caggacggta cgccggactg gaacctcatc gaacgtttac
    15721 tgaaagagtg gcagccggac gaaatcatcg tcggtttgcc gctgaatatg gacggcaccg
    15781 agcaaccatt gactgccaga gcgcgtaaat ttgccaaccg tattcatggt cgtttcggtg
    15841 ttgaagtaaa gctccatgac gaacgtctta gcaccgtgga agcccgttcc ggtctgtttg
    15901 aacagggcgg ctatcgggcg ctcaacaaag gcaaagtcga ctctgcctct gcggttatta
    15961 ttctcgaaag ctatttcgag cagggatatt aaggcgattt aaaagcctgg cggatagtcg
    16021 ttaccgccat cttgccggat gcggcgtgaa cgcctatccg gcctacatgt ggcagcgatt
    16081 gtaggtctga taagacgcgc aaacgtctca tcagacgttt attgcctttg taggcccgat
    16141 aagcttgcac atcgggcatg gcaacgtcac aaacgccctt cccccactcg ctgctgaaaa
    16201 ctctgctgaa acgttatcat ccccacctgc tgcccggttt gaataacatg cggtaactgg
    16261 tgggttttcc cttcgcgaat caaattcccc accgcgggag tgttaatcaa taattcaaac
    16321 agcgccacgc gtccttcctg tttatccact tccagctttt gtgacaacac ggcccgtaaa
    16381 ctacctgcca gttgattacg tacgggatct ttttcctgcg ccggaaatga atccaccagt
    16441 cgctcaactg cctgcgcggc accacgcgta tgtaatgttg ccagcaccaa atgcccggtt
    16501 tctgccgccg tcagtgccag acggattgtc tcgctgtcac gcagctctcc gagcaaaatc
    16561 acatcaggat cttcccgcaa tgcggcccgc aatcccgatg cgaacgtcat acagtgcaaa
    16621 ccaatttccc gcttctggat caaacatcgc tggctggcat agatatattc aacaggatct
    16681 tccagcgtca gaatatgcgc atcggcatgt tgattgagat agccaaccat cgccgccagc
    16741 gtggtagatt tgctgctccc cgtcgccccc gtcaccagaa tcaggccatt ctcactcttg
    16801 agtaattccg gcaataccgt tggtgcgcca agctgttcga gctgcgggca gtgcgaaggt
    16861 aacagccgta acgccagcga aatgccatgc cgttgcgcaa atgcgctgcc acgcaatcgc
    16921 tggttttccg ccagcgacac ggcaaaatcc agctggccat tttccagcaa tattgcccgc
    16981 tgatcgtcat ccagccactc ccgcagtagc tcttcgacgt ccggcgcctc aaacggcgca
    17041 gcttccatta gcccgcgaat gcgccatcgt gcgggccagg cgctgcacag gtgtagatcc
    17101 gagacgttat gctttacact aagggccaca atttcttcca tattcatact aagatcctcg
    17161 gaaaatgaac gatattgcgc ataacctggc acaggtccgg gacaaaatct cagcggctgc
    17221 aacgcgttgc ggccgttctc cagaagaaat tacgctgctt gcagtcagta aaacaaaacc
    17281 tgcgagcgcc atcgcagaag ccattgatgc cgggcagcgt caatttggtg aaaactacgt
    17341 tcaggaaggg gtagataaaa ttcgccactt tcaggaactg ggcgtaacag gattagaatg
    17401 gcattttatt ggcccgttgc agtctaataa aagccgcctg gtggcagagc atttcgactg
    17461 gtgtcatacc atcgaccgtt tgcgcattgc tacccgcctc aacgatcagc gcccggcaga
    17521 acttccccct cttaacgtac tgattcaaat taatattagt gatgaaaaca gtaagtccgg
    17581 gattcaactg gcagaactgg acgagctggc agcggcggtc gctgaactac cgcgtttacg
    17641 tctgcgcggg ctgatggcaa tccctgcgcc tgagtcagaa tatgtaaggc agtttgaagt
    17701 tgcacgccaa atggctgtag catttgccgg actgaaaacg cgctacccgc atatcgacac
    17761 gctctctctg ggaatgtcgg acgatatgga agccgccatt gcggcaggta gcacgatggt
    17821 tcgtatcggc actgcaattt ttggtgcgcg tgattactct aaaaaataag gaattaaagg
    17881 aacgccatga atacgttgac tttcctgctt tcaacggtca ttgagctgta taccatggtg
    17941 ctgttattac gcatctggat gcagtgggct cattgtgatt tttacaaccc cttctcacag
    18001 tttgtagtga aggtaacgca gccaattatc gggccactgc gccgcgttat tccggcaatg
    18061 ggccccattg acagcgcctc gctgctggtt gcctatattc tcagttttat caaagccatc
    18121 gtgctgttta aagtggtgac cttcctgcca atcatctgga ttgctggttt actgattctg
    18181 ctgaaaacca tcggcctgct gattttctgg gtcctgctgg tgatggcgat tatgagctgg
    18241 gtaagccagg gtcgtagtcc gattgaatac gtgctgattc agctggccga tccgctgctg
    18301 cgcccgattc gccgcctgct accggcaatg ggtgggattg atttctcgcc gatgatcctc
    18361 gttctgctgc tgtatgtcat caatatgggt gtcgcagaag tattacaggc aaccggaaac
    18421 atgctgctgc cggggctgtg gatggcgtta tgagtgccgt aacagttaat gatgacggtc
    18481 tggttttacg gctctatatt cagccgaaag ccagccgtga ttctattgtc ggtttacatg
    18541 gcgacgaagt taaagtcgcc attaccgcgc cgccggttga cggccagggc aacagtcatc
    18601 tggtgaagtt tctcggtaag caattccggg ttgccaaaag ccaggtggtg attgaaaaag
    18661 gcgaacttgg ccgccacaaa caaattaaaa tcattaatcc gcaacaaatc ccgccagaaa
    18721 tcgtggcgtt aattaattag gtatcctatg caaaaagttg tcctcgcaac cggcaatgcc
    18781 ggtaaagtgc gtgagctggc gtcgctgctt agcgacttcg gtcttgatat cgtggcccaa
    18841 acagacctcg gcgtagattc cgctgaagaa accggcctga cctttatcga aaacgcgatt
    18901 ctgaaagcgc gctatgcggc aaaagtgacc ggtttaccgg caattgccga cgactctggt
    18961 ctggcggtag atgcgctggg tggcgcgccg ggtatttact ccgcgcgtta ttccggtgaa
    19021 gacgcgaccg atcaaaagaa tctgcaaaaa ctgttggaaa cactgaaaga cgtaccggac
    19081 gaccaacgtc aggcgcgttt ccactgcgtg ctggtatatc tgcgtcacgc ggaagatccg
    19141 actccgctgg tatgccacgg cagctggccg ggcgtgatta ctcgcgaacc agcgggcact
    19201 ggtggctttg gttatgatcc aatcttcttc gtaccttccg aagggaaaac cgccgccgaa
    19261 ctgacccgcg aagaaaagag cgccatttcc caccgtggtc aggcgttgaa actgctgctg
    19321 gacgctttac gtaatggtta aattaccgcc gctgagtctc tacattcaca tcccgtggtg
    19381 cgtgcagaaa tgcccgtact gcgatttcaa ctctcacgcg ttgaaaggag aagtgccgca
    19441 cgacgattat gttcagcatc tgctttgcga tctggacaat gatgtggctt acgctcaggg
    19501 ccgtgaagta aagacaatct ttattggcgg tggtacgccg agcctgcttt ccggcccggc
    19561 gatgcaaacg ctgctggacg gcgtgcgtgc gcgtttgccg ctggcagcgg atgcagaaat
    19621 tactatggaa gcgaaccctg gaacggtaga agccgatcgc tttgtcgatt atcagcgtgc
    19681 tggtgtgaac cgcatctcta ttggtgtgca gagttttagc gaagaaaagc tgaaacgact
    19741 tgggcgtatt catggcccgc aagaagcgaa acgcgcggca aagctggcga gcgggctggg
    19801 gctgcgtagc tttaacctcg atttgatgca tggactaccg gatcaatcac tggaagaggc
    19861 gcttggcgat ctacgccagg ccattgaact gaatccgccg catctttcct ggtatcaact
    19921 gaccatcgaa cccaatacgc tgtttggttc gcgaccaccg gtgctgccgg acgatgacgc
    19981 gctgtgggat attttcgaac aggggcatca gttattaacc gcagcgggtt atcagcaata
    20041 tgaaacttcc gcttacgcca aacccggtta tcagtgccag cacaatctca actactggcg
    20101 atttggtgac tacatcggta ttggctgcgg cgcgcacggc aaagtcacct tcccggatgg
    20161 gcgcattctg cgtaccacta aaacgcgtca tcctcgtggt tttatgcaag gaaggtatct
    20221 ggaaagccag cgtgatgtcg aagccgcaga taagccgttt gagttcttta tgaatcgctt
    20281 ccgtctgctg gaggccgcgc cgcgcgtgga gtttattgcg tataccgggc tttgcgaaga
    20341 tgtgattcgc ccacagttag acgaggcgat tgcccagggt tatctcaccg aatgtgcgga
    20401 ttactggcag ataaccgagc acggtaaatt attcttaaac tcacttcttg aattattcct
    20461 cgctgagtaa tcattaatgc gcctgcgggc gcattaatca cttctttccc ccccgttaat
    20521 taacttcggc aaaatatatt tcttactgaa taaacatcga tgatatttat tagatatatc
    20581 tagtgaatat ttactcatca tttattgatt ataaacatgt acaacctggc gcacaaattt
    20641 aatatttaat ttctgacttt atttcaagtt gtgataaaca tagacaagtt gtcagatagg
    20701 ttaaccaatt tgtcgcacaa agcactgata agcacaatta gatatcaacc aatttccatc
    20761 cttcaacagg gagttttttt atgaaaatga ggtcatttac ccgctcatta gtctgcgcat
    20821 ccttgctggc tctggttagc actggcgtaa acgccgcaga aaaagtgacg ttaaagttag
    20881 cacacaacct ggaaagaagt catgttgtac atcagagttt tgaagagctg gcgaaagagg
    20941 taaaacagct gtcagaaggt aatatggtga tccgtattta tcccagcagc cagatgggta
    21001 atgcgcgaga aactatggaa ttgttgcaaa atggcgcact ggatatgacc aaaggttccg
    21061 ccagtgatct ggagtctttt gataatattt atgctatcta taatttgccg ttcttattta
    21121 aagatcaggc tcacttcaac aaagttgttt ttggtgaagt tggtaaagaa attatggact
    21181 caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtaca cgcagtttct
    21241 atgcgaaaaa accgattact aagccagaag atcttaaagg gctgaaaatt cgtgttcagc
    21301 caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact ccaatttcat
    21361 ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa aataacgtgc
    21421 cttcctgggt gcagactcga catattgaaa ttgccaaagt tttttctgaa gatgaacacg
    21481 cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta accccagaac
    21541 agaaacaaat cctcgaaaca gcagcgaaga aatccgaagc ttatcaacaa aaattgtggg
    21601 aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa attgtaaaag
    21661 tggataaagc accattccgt gccgcagttc agcctctgtt tgacgacttc aaaaaagatc
    21721 caaaacaagc tgctttgtta gagaagttcg acaacgctgc tcaataatat tccgggccag
    21781 tctctggccc tgattttcca agatgcttac aatgatattt aaccgcctga aacttgcggt
    21841 agaccgcgtc attgccgcgt tttctgtcgc cgtcatgctt gctctggttg tatgcgtagt
    21901 ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg atgagctggc
    21961 acgatttttg atgatctggg tagggttatt aggtg
//
LOCUS       DAETQN010000067        21684 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.067, whole genome shotgun sequence.
ACCESSION   DAETQN010000067 DAETQN010000000
VERSION     DAETQN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.067"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..182)
                     /locus_tag="K0W68_002540"
     rRNA            complement(<1..182)
                     /locus_tag="K0W68_002540"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(560..1105)
                     /gene="hemG"
                     /locus_tag="K0W68_002541"
     CDS             complement(560..1105)
                     /gene="hemG"
                     /locus_tag="K0W68_002541"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBI3074071.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(1117..2568)
                     /gene="trkH"
                     /locus_tag="K0W68_002542"
     CDS             complement(1117..2568)
                     /gene="trkH"
                     /locus_tag="K0W68_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBI3074072.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2607..3221)
                     /locus_tag="K0W68_002543"
     CDS             complement(2607..3221)
                     /locus_tag="K0W68_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBI3074073.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(3221..4552)
                     /gene="pepQ"
                     /locus_tag="K0W68_002544"
     CDS             complement(3221..4552)
                     /gene="pepQ"
                     /locus_tag="K0W68_002544"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBI3074074.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4742..6931
                     /gene="fadB"
                     /locus_tag="K0W68_002545"
     CDS             4742..6931
                     /gene="fadB"
                     /locus_tag="K0W68_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBI3074075.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6941..8104
                     /gene="fadA"
                     /locus_tag="K0W68_002546"
     CDS             6941..8104
                     /gene="fadA"
                     /locus_tag="K0W68_002546"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBI3074076.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(8294..8995)
                     /gene="fre"
                     /locus_tag="K0W68_002547"
     CDS             complement(8294..8995)
                     /gene="fre"
                     /locus_tag="K0W68_002547"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBI3074077.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(9042..10535)
                     /gene="ubiD"
                     /locus_tag="K0W68_002548"
     CDS             complement(9042..10535)
                     /gene="ubiD"
                     /locus_tag="K0W68_002548"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBI3074078.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10702..11190
                     /gene="rfaH"
                     /locus_tag="K0W68_002549"
     CDS             10702..11190
                     /gene="rfaH"
                     /locus_tag="K0W68_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBI3074079.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(11187..11969)
                     /gene="tatD"
                     /locus_tag="K0W68_002550"
     CDS             complement(11187..11969)
                     /gene="tatD"
                     /locus_tag="K0W68_002550"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBI3074080.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(12011..12787)
                     /gene="tatC"
                     /locus_tag="K0W68_002551"
     CDS             complement(12011..12787)
                     /gene="tatC"
                     /locus_tag="K0W68_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBI3074081.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12790..13305)
                     /gene="tatB"
                     /locus_tag="K0W68_002552"
     CDS             complement(12790..13305)
                     /gene="tatB"
                     /locus_tag="K0W68_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBI3074082.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13309..13578)
                     /gene="tatA"
                     /locus_tag="K0W68_002553"
     CDS             complement(13309..13578)
                     /gene="tatA"
                     /locus_tag="K0W68_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBI3074083.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13657..15297)
                     /gene="ubiB"
                     /locus_tag="K0W68_002554"
     CDS             complement(13657..15297)
                     /gene="ubiB"
                     /locus_tag="K0W68_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBI3074084.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15294..15899)
                     /gene="ubiJ"
                     /locus_tag="K0W68_002555"
     CDS             complement(15294..15899)
                     /gene="ubiJ"
                     /locus_tag="K0W68_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBI3074085.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15913..16668)
                     /gene="ubiE"
                     /locus_tag="K0W68_002556"
     CDS             complement(15913..16668)
                     /gene="ubiE"
                     /locus_tag="K0W68_002556"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBI3074086.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16763..18190)
                     /gene="rmuC"
                     /locus_tag="K0W68_002557"
     CDS             complement(16763..18190)
                     /gene="rmuC"
                     /locus_tag="K0W68_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBI3074087.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18331..19092)
                     /gene="udp"
                     /locus_tag="K0W68_002558"
     CDS             complement(18331..19092)
                     /gene="udp"
                     /locus_tag="K0W68_002558"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBI3074088.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            19354..20166
                     /locus_tag="K0W68_002559"
     CDS             19354..20166
                     /locus_tag="K0W68_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBI3074089.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20560..21621
                     /gene="hcp"
                     /locus_tag="K0W68_002560"
                     /pseudo
     CDS             20560..21621
                     /gene="hcp"
                     /locus_tag="K0W68_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5318 a         5804 c         5421 g         5141 t
ORIGIN      
        1 ttatgcggta ttagctaccg tttccagtag ttatccccct ccatcaggca gtttcccaga
       61 cattactcac ccgtccgcca ctcgtcagcg aagcagcaag ctgcttcctg ttaccgttcg
      121 acttgcatgt gttaggcctg ccgccagcgt tcaatctgag ccatgatcaa actcttcaat
      181 ttaaaagttt gatgctcaaa gaattaaact tcgtaatgaa ttacgtgtcc actcttgaga
      241 cttggtattc atttttcgtc ttgcgacgtt aagaatccgt atcttcgagt gcccacacag
      301 attgtctgat aaattgttaa agagcagtgc cgcttcgctt tttctcagcg gcgcggggtg
      361 tgcataatac gctttcccgc tacagagtca agcatttatt tttgctttct ctgtcggaat
      421 tctcaggaga accccgctga cccggcggcg tgtttgccgt tgttccgtgt cagtggtggc
      481 gcattatagg gagttattcc ggcctgacaa gaggaaattt aaaataattt tctgaccgcg
      541 cattttttat tctttatgct tatttcagcg tcggtttgtt ggttaactgg gcgatttctc
      601 gggcgaaatt cgccacctgc tcccaatcgg tatagacaac ttctttgcgc gtatccgttt
      661 caccgcctga catcttcata attagcttga tcataaaacg atcgtaccag cgataacgtg
      721 ggtaacgcag cgccccggca atgaccgcgc agcgatcggg acgccattgc gagttcatca
      781 gaaacttccg cgcgtagctg ttggtctgtg gagtacgctt ctccggtttg cgcgccacca
      841 ggtttacgga ataaaaggcg ctcggcatcg aattcagtcg cgtcgcatgt tttttgacaa
      901 attcctggaa cgcagagtgg tagtgaccat agcgaataga agcaccaatg accacacggt
      961 cataattttc ccattgtggt tcttcaatgc ggtgcacatt ggcgacatcc gtctggatcc
     1021 ccagttcttt cagttccgaa gccaggtagg aggcaatctc gcgcgtttgt ccgtcccttg
     1081 ttgagaaaag aattaatgtt ttcacgtatt actccatcat tcacgccaga aagtcggggt
     1141 aaagagcacc agcaatgtaa agacctcgag acgaccaaac agcatgttgg caatcaggat
     1201 ccatttagcc accgggttca tactggtaaa gttatcagca accacgccaa gccctggccc
     1261 caggttattc aatgtcgcaa caactgaagc aaaggcagaa aagtcatcca cgcccgtggc
     1321 gataatcgcc agcatactga caataaacac caatgcatag gcggagaaaa atccccaaac
     1381 ggcttcgagg atacgttccg gcagtgcgcg attccccagc ttaatgctat agacggcgtt
     1441 cggatgcacc agtcgtttca gctcacggtt cccctgctta aacagcagca ggatgcggat
     1501 cactttcagg ccaccgcccg ttgacccggc gcaaccgccg ataaaagctg aacataaaag
     1561 cagtaccggc aaaaagagcg gccagcgggc aatgctgtca gtcgtaaacc cggcggttgt
     1621 cgccatcgat accacctgga aaaacgcctg gttaattgtc atcagcgccg aactgtagac
     1681 attatgaaac cacagtacga gtgtacaaat aaccaccagc gtaaactgta cgccgataaa
     1741 catgcgaaat tccggatcgc gccaataaac cttcagacta cgcccactta acagtgaaaa
     1801 gtgcagaccg tagttacagc cggagatcag caggaagata gcaatgatag tgttaatagt
     1861 cgggctgtcg aaataaccga tactggcatc atgtgtcgag aagccgccaa tagcgatagt
     1921 cgcaaagcta tggccgatgg catcaaaggc atccattccg gcaaaccaca acgccagcgc
     1981 acaggcgacg gtcagcaaga cataaatcaa ccacagggtt ttcgccgttt ccgcaattcg
     2041 cgggcgcatt ttgttatctt tcagcgggcc gggcatttct gcgcgataga gctgcatccc
     2101 acccacgccg aggataggca gtatcgcaac cgctaacacg atgatcccca tcccgccaaa
     2161 ccattgcagc atctggcgat aaaagaggat ggcatgaggg agcgaatcca gccccaccag
     2221 cgtagtggcc ccggtggtgg tcaggccaga gaaagattca aaaaacgcat cggtaatcgt
     2281 gaggttcggg ctttccgaga agataaaagg gagcgcaccg acactaccca gcacggtcca
     2341 gaacagcacc actatcagaa acccctcacg ggatttcagt tcgcccttct ctttacggtt
     2401 cggccaccac agcatagaac caatggcgag ggcgacaaaa aaggtctggg taaaagcgcg
     2461 gcccgctcca tcccggtaga tgagtgctac cagcccaggg ataatcatgg tccctgaaaa
     2521 taagatgacc agtagtccaa cgattcgggt aatggcgcga aaatgcatct ctgccgcttc
     2581 cttagttctg caaaacgaag tggggattat tcttcaatcg ctaacaattg caatgaacca
     2641 cggctaaaat ccgccagctt tgcggaaaat tcagccactt tcgccgccgg aagcgccacc
     2701 cgcagcagaa cgaatgcctg ataatcactg ttgataattt tgccgtcaca ctgccccagc
     2761 aacgcttcaa tgccggttaa ctgatgatat tcacattgca aagtatattc ggttaatggc
     2821 gtcttgcgtt gggtcgttag ctggcgcagc gcctgattca cgccgccgcc atacgctttc
     2881 actaacccac cggtgccaag caatatgccg ccgtagtagc gcactaccac agaggtaatt
     2941 tccccgacgc cgctgcccat tagctgggcg agcatcggtt tacctgccgt tcctgccggc
     3001 tccccgtcgt cagagaaacc cagctgttga gaatcatccg gcgcacccgc cacccacgcc
     3061 acgcaatggt ggcgggcatc ggggtgttct gcccgcaccg attcaacaaa cgctttcgcc
     3121 gcctcaacgc catcggtatg cgccaacatc gttatgaagc ggcttttttt gatctcttca
     3181 acgaccgtga ccggtgccgc aggaattaac cagctttcca tcacgccagt ttcagatccc
     3241 gggtcatgtt ttccacgttg ttttcgtgga tcaccacgtt gtcttcgata cgaatgccgc
     3301 cgaacggttt cagtgcttca attttctgcc agttgaagtg cttgctgaac tgcccttcac
     3361 gccacggtgc cagtagcgat tcaatgaagt agatacccgg ttcgatggtt aacaccatac
     3421 ccggctggag aatacgggtg cagcgcaggt acggatattt tgccggtgcc gcgaggtgtg
     3481 taccgctatc atcctgcata aaaccggcta cgtcatgcac ctgcaggccc agcggatgac
     3541 cgataccatg cggcataaac ggtccggtga gatcgttttc gaccatcgct tcttcactca
     3601 tatcggtgat gatttgatgt ttacgcagca gtttggcgat gcgctgatgg aactggatgt
     3661 ggtaatccac atagctaacg cctgctttca tggtggcgat cagcgccagt tgttcatcat
     3721 ttacgtcttt caccagctgt gcgtagtcgt tgtcgctttt tgccgaccag gtacgagtca
     3781 ggtcagccgc atagccgtta tattcggccc cggcatccag caggaagctg cgcatctctt
     3841 ccggtgcctg atgatccagt ttggtgtaat gcagcaccga agcgtgttcg ttaagcgcca
     3901 caatgttgct gtaaggtacg tcggtatcac gatgaccggt cgcggtcaga taggcgatgt
     3961 tgatatcgaa ctcgctcatg ccagaacgga acgcttcttc tgccgcacga tgaccgttga
     4021 ccgccatttt ctgcgcttca cgcatacagg ccagttcgta ctcggttttg aaggagcggt
     4081 agtaatgcag gtagtcgatc acccctttcg ggttgatgtt gctggcctca atacccagtt
     4141 gcagcgcacg ttccggcacc ggaccgatat aaccgatatt gccgcgcgca gcgggcagca
     4201 ggctaccaat gccatcggct ttcggcagtg cgatcacttc cacatcttca gtccagaagg
     4261 aggtcggcag cggttcgacg ttgtgccagt aatcaaccgg cagatagaac cacagtttcg
     4321 gcttgttcac gccatccacc agcaaccagc agtttggcac ctgagttacc ggcacccacg
     4381 ctttgaattg cgggttcact ttaaacggat agggatgatc gtcgagaaaa acgttgaaca
     4441 gctcgccgga gtgaataagt aatgcatcca gcttgaagcg cgccagcgca tcgcgagtcc
     4501 gttcttgtaa ggtagctata tgatttttat agagcgaggc cagtgattcc attttttacc
     4561 cttctgtttt tttgacctta agtctccgca tcttagcaca tcgttcatcc agagcgtgat
     4621 ttctgccgag cgtgatcaga tcggcatttc tttaatcttt tatttgcata tttttaacac
     4681 aaaatacaca cttcgatgca tctggtacga ccagatcacc ttgcggattc aggagactga
     4741 catgctttac aaaggcgaca ccctgtacct tgactggctg gaagatggca ttgccgaact
     4801 ggtatttgat gccccaggtt cagttaataa actcgacact gcgaccgtcg ccagcctcgg
     4861 cgaggccatc ggcgtgctgg aacagcaatc agatctaaaa gggctgctgc tgcgttcgaa
     4921 caaagcagcc tttatcgtcg gtgctgatat caccgaattt ttgtccctgt tcctcgttcc
     4981 tgaagaacag ttaagtcagt ggctgcactt tgccaatagc gtgtttaatc gcctggaaga
     5041 tctgccggtg ccgaccattg ctgccgtcaa cggctatgcg ctgggtggtg gctgcgaatg
     5101 cgtgctggcg accgattatc gtctggcgac gccggatctg cgcatcggtc tgccggaaac
     5161 caaactgggc atcatgccgg gctttggcgg ttctgtacgt atgccacgta tgctgggcgc
     5221 tgacagtgcg ctggaaatca ttgccgccgg taaagatgtc ggcgcggatc aggcgctgaa
     5281 aatcggtctg gtggatgggg tagtcaaagc agaaaaactg gttgaaggcg caaaggcggt
     5341 tttacgccag gccattaacg gcgacctcga ctggaaagcg aaacgccagc cgaagctgga
     5401 accgctaaaa ctgagcaaga ttgaagccac catgagcttc accatcgcta aaggaatggt
     5461 cgcgcaaacg gcggggaaac attatccggc ccccatcacc gcagtaaaaa ccattgaagc
     5521 tgcggcccgt tttggtcgtg aagaagcctt aaatctggaa aacaaaagtt ttgtcccgct
     5581 ggcgcatacc aacgaagctc gcgcactggt cggcattttc cttaacgatc aatatgtaaa
     5641 aggcaaagcg aagaaactca ccaaagacgt tgaaaccccg aaacaggctg ctgtgctggg
     5701 cgcaggcatc atgggtggcg gtatcgctta ccagtccgcg tggaaaggcg tgccggttgt
     5761 catgaaagat atcaacgaca agtcgttaac cctcggcatg accgaagccg cgaaactgct
     5821 gaacaagcag cttgagcgcg gcaagatcga tggtctgaaa ctggctggcg tgatctccac
     5881 gatccaccca acgctcgact acgccggatt tgaccgcgtg gatattgtgg tagaagcggt
     5941 tgttgaaaac ccgaaagtga aaaaagccgt actggcagaa accgaacaaa aagtacgcca
     6001 ggataccgtg ctggcgtcta acacttcaac cattcctatc agcgaactgg ccaacgcgct
     6061 ggaacgcccg gaaaacttct gcgggatgca cttctttaac ccggtccacc gaatgccgtt
     6121 ggtagaaatt attcgcggcg agaaaagctc cgacgaaacc atcgcgaaag ttgtcgcctg
     6181 ggcgagcaag atgggcaaga cgccgattgt ggttaacgac tgccccggct tctttgttaa
     6241 ccgcgtgctg ttcccgtatt tcgccggttt cagccagctg ctgcgcgacg gcgcggattt
     6301 ccgcaagatc gacaaagtga tggaaaaaca gtttggctgg ccgatgggcc cggcatatct
     6361 gctggacgtt gtgggcattg ataccgcgca tcacgctcag gctgtcatgg cagcaggctt
     6421 cccgcagcgg atgcaaaaag attaccgcga tgccatcgac gcgctgtttg atgccaaccg
     6481 ctttggtcag aagaacggcc tcggtttctg gcgttataaa gaagacagca aaggtaagcc
     6541 gaagaaagaa gaagacgccg ccgttgaaga cctgctggca gaagtgagcc agccgaagcg
     6601 cgatttcagc gaagaagaga ttatcgcccg catgatgatc ccgatggtca acgaagtggt
     6661 gcgctgtctg gaagagggca ttatcgccac tccggcggaa gcggatatgg cgctggtcta
     6721 cggcctgggc ttccctccgt tccacggcgg cgcgttccgc tggctggaca ccctcggtag
     6781 cgcaaaatac ctcgatatgg cacagcaata tcagcacctc ggcccgctgt atgaagtgcc
     6841 ggaaggtctg cgtaataaag cgcgtcataa cgaaccgtac tatcctccgg ttgagccagc
     6901 ccgtccggtt ggcgacctga aaacggctta aggagtcaca atggaacagg ttgtcattgt
     6961 cgatgcaatt cgcaccccga tgggccgttc gaagggcggt gcttttcgta acgtgcgtgc
     7021 agaagatctc tccgctcatt taatgcgtag cctgctggcg cgtaacccgg cgctggaagc
     7081 ggcggccctc gacgatattt actggggttg tgtgcagcag acgctggagc agggttttaa
     7141 tatcgcccgt aacgcggcgc tgctggcaga agtgccgcac tctgtcccgg cggttaccgt
     7201 caatcgcttg tgtggttcat ccatgcaggc actgcatgac gcagcacgaa tgatcatgac
     7261 tggcgatgcg caggcaggtc tggttggcgg cgtggagcat atgggccatg tgccgatgag
     7321 tcacggcgtc gattttcacc ccggcctgag ccgcaatgtc gccaaagcgg cgggcatgat
     7381 gggcttaacg gcagaaatgc tggcgcgtat gcacggtatc agccgtgaaa tgcaggatgc
     7441 ctttgccgcg cggtcacacg cccgcgcctg ggccgccacg cagtcgggcg catttaaaaa
     7501 tgaaatcatc ccgaccggtg gtcacgatgc cgacggcgtc ctgaagcagt ttaattacga
     7561 cgaagtgatt cgcccggaaa ccaccgtgga agccctcgcc acgctgcgtc cggcgtttga
     7621 tccagtaagc ggtacggtaa cggcgggtac atcttctgca ctttctgatg gtgccgcggc
     7681 catgctggtg atgagtgaaa gccgcgcccg tgaattaggt cttaagccgc gcgcccgtgt
     7741 gcgttcgatg gcggtcgttg gttgtgatcc gtcgattatg ggttacggcc cggttcctgc
     7801 ctcgaagctg gcgctgaaaa aagcggggct ttctgccagc gatatcggcg tgtttgagat
     7861 gaacgaagcc tttgccgcgc agatcctgcc gtgcattaaa gatctcgggc taatggagca
     7921 gattgacgag aagatcaacc tcaatggtgg tgcgatcgcg ctgggtcatc cgctgggttg
     7981 ttccggtgcg cgtatcagca ccacgttgct gaatctgatg gaacacaaag acgttcagtt
     8041 tggtctggcg acgatgtgta tcggtctggg tcagggtatt gcgacggtgt ttgagcgggt
     8101 ttaaccactt gccggatgcg gcgtgaacgc cttatccggt ctaccgatcc tgcaccaatg
     8161 taggcctgat aagacgcgcc agcgtcgcat caggcatcgt gcaccgattg ccggatgcga
     8221 catagatcta aatgccactg aataaagttt tagttgccgt tcttcccgcc tgtcaggggc
     8281 gggtttttat atctcagata aatgcaaacg catcgccaaa cagacgatct tcccgcgcat
     8341 tacgctcact gcaaaacagg tcacgagcaa ttttcgccat ctcaaaacgt ccggcaatat
     8401 agatatcatg ctctgccagc gtaccatgat cctgcaagac cgccgttaac acggtgccag
     8461 tacgcccacg ccagcccgct tccggttgtt caaccaccgg caccacttgc agaccaggat
     8521 gcttcagcga aagcgcctca agctcgcaga gatcatacag atgttgctct tcacgcccac
     8581 cccagtaaat ggtgatatcg cgattgggat tacgcgccaa cgccgtcaat aaaatcgagc
     8641 gcgcatagga gaacccggtg ccgcccgcaa tcaaaatcat cggacgctct tcatcatcgc
     8701 gcagccacgc ttctccgtgg ggaatatcga ccacgatttg atgatctttg aggatgcggt
     8761 ccatgactgc tttcgcgtaa aggttgattt cagaagcgcc aatatgcagc tcgataaacc
     8821 ctttttcatc cggcgtcgaa gccattgaaa acggacgttt gtcgcgctca tccatcacta
     8881 ccatcaaata ctgaccagca cgaaaagaaa aggccgcgtc tggtacgatt cggacacggt
     8941 atacagtatc cgtgatagct tctaccgagg tcactttaca gcttaaggtt gtcatgcgct
     9001 ttctctgtcg gatcaaataa atagggcaaa actaacgcgc atcaggcgct tttaccgttg
     9061 ttaaaaatag ccagttcatc ccagatggcg tcaatatgcg cgacgacatc aggatctttt
     9121 ttgatgggac gtccccattc acgctgggtt tcccccggcc atttattcgt ggcatccagc
     9181 cccatttttg aacccagccc ggagacaggc gaggcaaaat ccagataatc aataggcgta
     9241 ttttctacca gaacagtatc ccgcgccggg tccatacggg tggtaatcgc ccaaatcaca
     9301 tcgttccagt cgcgtgcgtt gacgtcatca tcgcaaacga tcacaaattt agtgtacata
     9361 aactggcgta agaacgacca gacgcccatc atgacgcgct tcgcgtgtcc ggcgtactgt
     9421 tttttgattg tcactaccgc cagacgataa gaacagcctt ccggtggcag gtaaaaatcg
     9481 acaatttccg ggaactgttt ttgcagaatc ggcacaaaca cttcgttcag tgcgacaccc
     9541 agcaccgcgg gttcatccgg cggacgacca gtataggtgg agtggtaaat cgcatcttca
     9601 cgctgggtaa tatgcgtcac ggtaaatacc gggaaactat cgacttcgtt atagtaaccg
     9661 gtgtggtcgc catacggccc ttccggcgca gtttcgcctt gttcgatata cccttccagc
     9721 acaatctccg cactggcggg cacttcaagg tcattggaga tacacttcac cacttcggtc
     9781 ttggtaccgc gcagcaatcc ggcaaacgca tactctgaaa gtgtatccgg aacgggggtg
     9841 actgcaccga gaatcgtggc gggatcggca cccagcgcca cagaaaccgg gaaacgttcg
     9901 cccggatgcg ccgcacacca ctcctgataa tccagcgcgc cgccgcgatg cgacagccag
     9961 cgcataatca gtttgttttt accaatcagc tgctggcgat aaatgcccag attctgccgc
    10021 tctttatgcg ggccgcgcgt tacggtcagc ccccaggtaa tcagcggcgc ggcatcttcc
    10081 ggccagcagg tcataatggg aatgcgattg agatcgacgt catcgccaga gacgattttt
    10141 tgttggcagg gcgcaccacg cagccgcttt gtcggcatgt tcaatacttg cttaaactgc
    10201 ggcagtttat caaacaggtc gcggaaacct tttggcggct cgggctcttt cagaaacgcc
    10261 aataatttac caacttcacg cagcgccgaa acatcttcct gccccatgcc catcgccacg
    10321 cgctttggcg taccgaacag gttgcacagc accggcattg agtagccttt agggttttcg
    10381 aacaacagcg caggcccacc ggcacgcaaa gtgcggtcag caatttcagt gatttccaga
    10441 tgcggatcca ccgggagcgt gatacgtttt agctcaccct gctgttcaag cagcgtcaag
    10501 aagtcgcgta aatcgttata tttcatggcg tccattgtag cctcttaatc tacgcccatt
    10561 atacggcgtt catctttgcg atgctgtaaa tttgttaaat tagcgtgaac tctgacggta
    10621 taacgcaaac cggggaatat aattaactta gcgtaaagct tttgctatcc ttgcgccccg
    10681 attaaacgga taagagtcat tatgcaatct tggtatttac tgtactgcaa gcgcgggcaa
    10741 cttcaacgtg cccaggaaca ccttgagaga caggctgtga attgcctggc accgatgatc
    10801 accctggaaa aaatcgtgcg tggaaaacgt actgcagtca gtgagccatt gtttcccaac
    10861 tacctgtttg tcgaatttga tccagaagtg attcatacca cgactatcaa cgcgacccgc
    10921 ggtgtcagcc acttcgtgcg ctttggcgcg tcgctagcga tagtcccatc ggcggtgatt
    10981 catcagctat cggtatataa accgaaagac attgtcgatc cggcaacccc ttatccggga
    11041 gataaggtga ttattaccga aggcgcgttc gaaggctttc aggccatttt caccgaaccc
    11101 gatggtgagg ctcgctccat gctattgctt aatcttatta ataaagagat taagcacagt
    11161 gtgaagaaca ccgagttccg caaactctaa aacgcaatcc caaaccgtgt tttgacatta
    11221 gcatccgtgg tggcagccag ccatgcggca tcttctccac gccagtgcgc aatacgttgc
    11281 aaaatatggg gcagatgggc tggctcgttg cgccgggatg atggctttgg cgtgagatcg
    11341 cgagggagca gatacggcgc atcagtttcg atcagtaatt tttccgccgg aatcaacggc
    11401 aaaagttccc gcagctccag tccgcgtcgt tcatcgtaaa cccaaccggt aatgccgata
    11461 taaattccat gcgccacgca cgcctgcatc tcttcgcgtg tgccggtaaa gcaatgaaga
    11521 accgcaccag gcagtttatc cagccacggc tccagcaatg tcataaaccg ctcgtgggca
    11581 tcgcgacagt gcataaatac cggcatgttt aaatctgcgg caatgcgtag ctgggcaaca
    11641 aaagcgcgtt cctgctcttc cggcgtcgaa aagttgcggt taaagtcgag accacattca
    11701 ccaatcgcca ccacttctgg ctgcgcggcc agctcaataa tcgcttcttc agtcgcagct
    11761 tgccactggc tgctgtcgtg aggatgtacg cccgccgttg accaacagga cgaatactga
    11821 cgcgccagct tttgcgcctg ctggctttca cgcaggttag tgccggtgat gagtagccca
    11881 ttaactcccg cgtcaaaagc gcgcgctaca acatcatcac ggtctttcgc aaattgcgaa
    11941 ctggtcaaat taacgccgat atcaaacatc ctgtactcca tatgacaacc gccctgacgg
    12001 gcggttgaat ttattcttca gttttttcgc tttctgcttc agcgtcgttt tcctcttccc
    12061 gatttcgccc tttaccaacg taaaagcgtg agaagaagac accgatttca aacagacagt
    12121 acatcgggat cgccaatagc gtttgcgaga agacatccgg cggcgtcagc aacatcccga
    12181 caacgaatgc accaaccagc acatacgggc gttttttgcg taagtcttct ggcgaggtaa
    12241 tccccatcca gcacagcagc acaattgcca ccggcacttc aaaggagaca ccaaacgcca
    12301 taaacagcgc cataacgaag cttaaatagc tggcgatgtc ggtggatacc tgcacccctt
    12361 ccggcgcggt attggcaagg aagccaaatg ccagcggaaa gaccacaaag taggcgaatg
    12421 ccatgccgat ataaaacagc agagagctgg aaaccagcag cggcaccacc aggcgacgtt
    12481 catgcttata cagcgctggg gcgataaatg cccacacctg atagagaatc accggcgctg
    12541 acagaatcag cgacaccata aaggtcagct tgatcggcgt aaagaacggc gaggccacgt
    12601 cggtggcgat catcgttgaa ccttgcggca actgcttgat caatggcgcg gataccaggt
    12661 gatagatgtc attggcgaaa tagaccagac acaggaatat cacgatcacc gcgataatgc
    12721 agttcagcag acgcttacgc agctcaatta gatgcgtgat aagcggttga gtatcttcta
    12781 cagacatgtt tacggtttat cactcgacga aggggaaggt gcagcggttt tcggttcagc
    12841 gtccgcagca ggttttacca ccggctctgg cgtggtttct ggcttctgtt ccggcgaact
    12901 ggcctgcgtt tgtgcagcgg caggcgttac gccctcatgc gcagcttcat tatctttcac
    12961 caccgggtta tggatggtgt gcgcttcatc gctcgccttt tcaggatcgt ttgcaacgta
    13021 ggaacgcttc atcgactccg cggcctggcg tagttcatcc atcgacgctt tcagttcggg
    13081 cgtcaggtta gtgaggctcg ccttttcaac ctttttcaga ctgtcctgaa actcctggag
    13141 ttttaactcc tgggtcagtt cgttctgcac cgttgtcgcc agtgaacgca acgcgcgaat
    13201 ccagcccgct accgttttta ccgccacagg cagtcgttgc ggccccagaa cgacgaggcc
    13261 gatgatgaac accaatagca gttcgctaaa accgatatca aacacggatt acacctgctc
    13321 tttatcgtgg cgcttcgcgt cttctgtttt agcctgttcc tgattcgtat ccgcctgctt
    13381 atcggcgata gttttcgcag taaaatcagc atcctgactg gttttatcct gctttggttc
    13441 atcatcgctc attgcttttt taaagccttt gatcgacgca ccaagatcgg aaccgatgga
    13501 gccgagcttt ttggtgccaa aaagcagtac aacgatgacg gcaataatca ataactgcca
    13561 aatactgata ccacccatac atgttcctct gtggtagatg atgattaaac aaggccgcat
    13621 tatacgttac acgacccgcc ttgagcgatg aaaaaatcag cgtgttttgc gccaaccgac
    13681 aaaccaggcg atcagaccac ctgccattaa ccagccgggc atcagccccc attcaggtcg
    13741 gctgaccaac aagaatgtgc cacttaatac taacgtagcg ccaattccga gaaaataacg
    13801 cgattgtccc tgacgtacat gatttgactg aagctcgcgg gcaatcttat caacactgtg
    13861 ctgtaaatac ttgccctggc gcaaactgtc gtaaaccaat tcaggcagtt ctggcatttt
    13921 ttcgacccag aacggcgctt tttctttaaa tgctctcacc agggcaggaa taccgacctg
    13981 atctttaatc cacgactcca ggaaaggctt cgctgttttc cataaatcga gctgcggata
    14041 aagctggcgt cctacccctt cgacatagag cagggttttc tggagtaaca ccagttgcgg
    14101 ctgcacttcc atattgaagc gacgcgccgt attaaacaga tttaacagta catgtccaaa
    14161 cgaaatttcg gccagcggtt tctcaaagat aggttcacag accgtacgaa tggcaaattc
    14221 gaactcttca acgttggtat ctggtggcac ccagccagaa tcgacgtgta gctctgccac
    14281 tttgcgatag tcgcgattaa agaaggcgat aaagttttct gccagatagc gtttatcttc
    14341 tttgtttagc gagccaacaa tcccgcaatc aatgccgata tatttcgggt tttccgggtg
    14401 ttcatagctt acgaaaatgt tgccagggtg catatcggca tggaaaaagc tgtcgcgaaa
    14461 gacctgagtg aagaacacct gcacgccacg ttccgccagc aatttcatgt tggtgccatt
    14521 tttctccagc gtcgcaacat cagacaccgg aatgccgtaa atgcgctcca tcaccatcat
    14581 cccttcacta caatagtcag ggtaaacttc cgggatgtag agcatcgggc tgtcttcaaa
    14641 atttcgccga agctgaatgg cgttggcaga ttcccgcagc aaattcagtt catcaatcaa
    14701 ggtcttttcg tactcgcgca ccacttcggt tgggcgcaga cggcgaccat ccggcagcaa
    14761 acgcggcacc cagcgagcca gacggtagat aagtttcaga tccgctttaa taaccggcaa
    14821 aatatccggg cggatgactt taatcaccac ctctttacca ttcgatttca atcgcgcggt
    14881 atgaacctgg gcgatagaag cagaagccag tggcttgatt tcaaaatcgt caaaccacgc
    14941 ttctaccggc aagccgccca ttgcagcttc aatctgctgc ttcgccagct tgccatcaaa
    15001 cggagcaact ttgtcctgca ataacgccag ctgatcggca atatgcggtg gaaaaagatc
    15061 gcggcgggtt gataacattt gcccgaactt gatccaaacc ggccccagtt cttgcagggc
    15121 cagtcgtagt cgctcaccta aaagtttgtc tttatgccga tttggcatcc agaataatga
    15181 gtatcgccat agccgtagcg gcagggtgat acgcattttg gggatcagtt catcaagtcc
    15241 gtagcttaaa aaagtgcgaa tgatgaaata taggcgccgt acttcacctg gcgtcattta
    15301 gcctccagtt tttccagccg tttggtcagg gcatcaacag cacgctcgac ggcagccgtc
    15361 tcttccgcaa accaggccac ttcaagcgga ccgggtgcca tacgccactc ttcagtaatg
    15421 gcttccgcca cataacgttg ctggcgctta atgccgtgat gcaggaactt tgcgcctccg
    15481 cgcatggctt tgctgattcc ttcagcggcg atatcaccgg tataaggggc cagcagttcc
    15541 gcagggtcga actctgccag atctgccagc gcaacgaagt tttgcaccac ctgaatatcg
    15601 ccctgcactt ccagctcacc actgcgaatc agtgcggtaa gctgctggcg atcgcgaagt
    15661 ttcggcaaca cactggcgta ggcgataacg gtgcagtcag catcgcctgc ccattcgccc
    15721 agtacatcaa cctggcgttc gctgaacacc agaatcaatg acgtcgaaaa gccttttacc
    15781 tccacgcgca atactttacc cagcagacgc gagcgggctg ttttcagcgc gggtgagcga
    15841 tacaggaagg tgttgagcag actttcaatt cctgccgtca ctaaaggttt aaaaggcatt
    15901 tccggtctcc tgtcagaact tataaccacg atgcagcgcc acaacccctg ccgtcagatt
    15961 gtagtagtcg acactttcga atccggcatc ctgcatcatg gctttcaggg tatcctgatc
    16021 gggatgcata cggatggatt ctgccagata acggtagctg tcggcgtcgt tcgcgaccag
    16081 tgagccaata cgcggcagca catggaagga gtatgcatca taggctttgc tcagcggctc
    16141 gataattggc ttcgagaact caagcaccag caggcggccg ccgggtttca gcacgcgata
    16201 cattgaacgc agtgctttat ctttgtcggt gacgttacgc agaccaaacg aaatggtgat
    16261 gcaatcaaag gtgttatccg ggaacggcag cgcctcagcg ttcgcctgaa catactcaac
    16321 gttgccaatc acaccgatat tacgcagctt ctcgcgtccc attttgagca tggattcatt
    16381 gatatcagca aggaccactt tgccagtttc tccgaccagg cgggagaatt tcgctgtcag
    16441 gtcgccggtg ccaccagcca gatccagcac ggtctgccca cggcgtacgc cgctgcaatc
    16501 aatcgtgaat cgcttccaca aacgatgaat accaaatgac atcaaatcat tcatgacatc
    16561 gtattttgat gccacggaat ggaaaacgtg ggcgaccata tccgcttttt gttccttcgc
    16621 gacggtctga aaaccaaagt gcgtcgtttc ttgtgactta tccaccatct caatgcctgc
    16681 tcatcaaaaa attgttccag aagtgtaaca gattgggcgt cgatgcccta gatttctacc
    16741 cggcttaact acccctaatg ggctagcgcg actgctgatt atattcatca tcgcgttgat
    16801 aagcttcatc attcggctgc tccggaaccg accgaagtcg atactcttca ttctggctca
    16861 ccgcctgttc agccaaatcc ggattaatct cgcgtttaat ttctactcct aaaccgcgaa
    16921 acgcttctgc ctgcgccagc acatttccgc gacctgaaga gagttttttc attgcctggc
    16981 ggtagttatc ctgcgctttg tcgagacttt gaccaatcgc ggacatgtca tcgatgaaca
    17041 aacgcatctt gtcgtacagc ttgctggcac gatcggcgat ttgctgggcg ttgcggcttt
    17101 gatgctcata acgccacagg ttggcaatag tacgcaacgc caccagcagc gtagtcgggc
    17161 taaccagcat gatattgttt ttcaacgctt cggtgatcag ctccggctgg cggtcaagcg
    17221 ccagtaaaaa agcgggttca acgggaataa acatcagcac gtaatccaga gttcgcagcc
    17281 ccggcagctg ttgataatct ttgcgtccca gcaaacggat atggttacgc accgacgcga
    17341 tatgttcctg tagcgcgctt tcgcgggtgt agtcgtcttc ggcgttaaaa tagcgttcat
    17401 aggcgaccag cgtcattttg gcgtcgatca ccacatcttt tccctgcggc agccgcacga
    17461 tgacatccgg ctgcatccgc gagcgggcgt cattttcgat gctgacctgg gtttcatatt
    17521 catacccttc acgcagaccg gaagcctcca gcacccgcgt caatactacc tcgccccagt
    17581 tgccctgggt tttattgtcg cctttcagcg cgcgcgtcag gttgatcgct tcctgggcca
    17641 tttgcgcgtt gagttgctgg agattgcgaa tttcgtgggt cagggtatgg cgttcttgtg
    17701 cttctttacc gaagctgtcc tgaacctgac ggcggaaacc gtccagttgt tcacgtagcg
    17761 gcgacaacag gctgttcaga ctctgacggt tttgctcatc aacccggcga ttgctgtgct
    17821 caaaaatacg gttggcgagg ttttcaaact gctcactgag gcgctgctcg ctgttaatca
    17881 tctggcgaat tttatcgtca gcatgttgct gtgcggcttc catccgcgtg gttacttcac
    17941 gcagatcggc ctccagagag gtgttaatac tttgcaggct gcgcacttcg ttattgagta
    18001 actcgcactc tgcacgccag tgctcgcttt gggtaatttg ttgttttgcc gcgcttaact
    18061 ccgcgaccat ctcttcacgt tcagctaatt gctcggcttt ttgctgcgca tgttgataac
    18121 tggcaaacag ccagccaatt gccacaccca ccaacgcaat aactgcgtaa accatgattg
    18181 agaaatccac aacgcctcct gacgcaacaa cttacccgca caaaatagaa ttgtgctgta
    18241 caaacgtcca gttggaaagg catttcctgc gaggcgctac gcaaaaatac aaaaggccga
    18301 acgcgtcggc cttcagacag gagaagagaa ttacagcaga cgacgcgccg cttccaccac
    18361 gattttcacc gcttggcttt cagtttgttt catcgtctca gcattcggga tctcttgctg
    18421 ggtgcggtta acgataacac ccgctaccat accggcacgc aggccctgac ttgcacacat
    18481 ggtcagcagg gttgcagatt ccatttcata gttcattatg cccatcgcct gccactcttc
    18541 catagaacct ttaaagtgac gaactacgcg accagagtaa gtatcgtaac gttcctgacc
    18601 tgggtagaag gtatcagaag aagctgtcac gccaacgtga gttgtcgcgc caatggattt
    18661 cgcagcttca accagcgcag tcgtacattc gaaatcagcg acagccggga attccagcgg
    18721 tgcgaagtgc aggctcgcgc catccagacg gacagacgcc gtggtaacca ggacatcacc
    18781 cacattaata tgcggctgaa tagcgcccgt tgtaccgata cgcaggaagg tgcgaatgcc
    18841 cagctgtgcc agctcttcaa cagcaataga ggtagacggg ccgccgatac cggtagagca
    18901 gacgataaca ggtttaccat ccagctctgc acgccaggta gtgaattcgc ggtgagatgc
    18961 cagcttaacc ggcttatcca tcagcgcggc gatcttttcc acacgatccg ggtcgccagg
    19021 gacgatggca agcgtagccc cttgtaaatc gtttttagtg aggccgagat gaaaaacatc
    19081 agacttggac atatacaact cctctgtgaa tcggtttagt cagaagaagc aaaaagacac
    19141 tttaccgaag ggtttaacat tttttcgtga tactcatcac catgacgcaa atgcgttgca
    19201 taaatttacc caccaaaagt gattcaaatc acataaatca ctcttcattc tgcaggactg
    19261 cataaaagat gagccgattc aggcaatgtg atttgttgca tgctgactat agttaacatt
    19321 gcgcaacatt tttataaggg tacggagaat accatggcaa caacacaaca atctggattt
    19381 gcacctgctg catcgcctct cgcttcgacc atcgttcaga ccctggacga cgcgattgtg
    19441 gcgggcttca cctctatccc ttcacaaggg gataacatgc ctgcttacca tgccagacca
    19501 aagcaaagcg atggcccact gccagtggtc attgtagtgc aggaaatttt tggcgtgcat
    19561 gaacatatcc gcgacatttg tcgccgtctg gcgctggagg ggtatctggc tatcgcacct
    19621 gaactttact tccgcgaagg cgatccgaat gattttgccg atatccccac gttgcttagc
    19681 ggtctggtag caaaagtgcc tgactcgcag gtgctggccg atctcgatca tgccgccagt
    19741 tgggcttccc gcaacggcgg cgatgttcat cgtttaatga tcaccggatt ctgctggggt
    19801 ggacgtatca cctggctgta tgccgcgcat aatccacagc taaaagctgc agtggcgtgg
    19861 tacggcaaac tgacgggcga caagtcgctg aattcaccga aacaacctgt tgatatcgca
    19921 accgatctta acgcgccgat tctcggctta tatggcggtc aggataacag cattccgcag
    19981 gagagcgttg aaaccatgcg ccaggcgctg cgggctgcta atgcgaaagc agagattatc
    20041 gtgtacccgg atgccgggca tgcattcaac gctgattatc gcccgagcta tcatgccgaa
    20101 tctgcgaaag atggctggca gcgtatgctg gaatggttta agcagtatgg agagaagcgg
    20161 agttagtttc ctcttagtct atatttgaga tggtgagtaa acgagtgcgt tttgctgcca
    20221 tctctgtccc ccaaaaaata atcagtatga attgattgga gattacgact ccgctatttg
    20281 accttaagaa agtcggcaag tcatagtcat aaatgagtct cttcaatgga agacacctta
    20341 gagatttacc atcaactccc attcacaaac aaacattaat tattatcaac acattcacat
    20401 taattataaa tcatctcaga ccttcctgat aaaaagatat taaagtgaaa cattttgttt
    20461 ttcacatttt agttcatcac ttttaggcgt aaataaaggt aaaaaaaaca acaatactat
    20521 ttctctttca caaaagaatt aacaaaaggt ttttcaagga tgagtgatat tatttacctg
    20581 accataaccg gagaacaaca aggatgtatt tcctctcgtt gtggaacgag cgcatcaata
    20641 ggtaatcgct ggcagattgg ccatgatgat gaaatcttcg cgttctcgct ctctaatagt
    20701 attacgaata caggcaaagg gtcataattg catggcctta gtttctgtaa attgattgac
    20761 aaaagctccc cgttacttat taacgctatt aacaataacg agcagttgtt catggagttt
    20821 gacttctatc ggatcaatag gtttggtcga tgggagaaat attacaacat tcaactccgc
    20881 ggcgcgctct tatcagcaat caatcacctg tttactgaga acaatctgga tacagaagcc
    20941 atcactgtca gttattaata tatactctcc aggcatctga ttgctaatac tgaatttagt
    21001 tattgggcat ttcccgataa ctacaaccgt ctgtttatac cacggccaaa aactaaagct
    21061 ggtaatggtt tcaaaacact taatagtaaa gcggttggca gactgcttgc tgctgtcggc
    21121 atatacaatg ggaatatcga aggattcagg gaaacagcag acaagttggg gggagatgca
    21181 cctgctggtt atgatcaatt tatggataac aaagggttac tcatcgctgg tgcttcaata
    21241 gttgctggag cagttctggg gagaatgaga ctccctgatt tggaatcact tgagcatttc
    21301 ggagccagag gagcagtatc aggaagacca tttaatcccg agcttgccgg tggtccaata
    21361 gaaaacctga cgacagatgg cgtaacaatc aacagggaag gtatcgctat tgttgaaaaa
    21421 catatagcca gatttggtca tgatcctgta aatgaagtga tgatcaatag actgaaagac
    21481 attgagaaag gaaaaatccc cccagaacaa gtagatctta acttctatac tcatgagtgt
    21541 cgggaatatc agcgttactg taatcttggt tgggaaacag gacaacctga tggtgacgca
    21601 ggctatgcac tctggaatca tactggattt gcccctatat ttccagacat ctgttatcac
    21661 ttaacccatt acaagcccgc tgcc
//
LOCUS       DAETQN010000068        21644 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.068, whole genome shotgun sequence.
ACCESSION   DAETQN010000068 DAETQN010000000
VERSION     DAETQN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.068"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(19..378)
                     /gene="traA"
                     /locus_tag="K0W68_002561"
     CDS             complement(19..378)
                     /gene="traA"
                     /locus_tag="K0W68_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HBI3074090.1"
                     /translation="MNTVLSVQGASAPVENKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(412..651)
                     /gene="traY"
                     /locus_tag="K0W68_002562"
     CDS             complement(412..651)
                     /gene="traY"
                     /locus_tag="K0W68_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HBI3074091.1"
                     /translation="MGGRVIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNE
                     ALVRLQDHLQRYPDFYNEEIFREVIEENESTFKEL"
     gene            complement(759..1406)
                     /locus_tag="K0W68_002563"
     CDS             complement(759..1406)
                     /locus_tag="K0W68_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HBI3074092.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFGCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
     gene            complement(1598..1981)
                     /locus_tag="K0W68_002564"
     CDS             complement(1598..1981)
                     /locus_tag="K0W68_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HBI3074093.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
     gene            2314..2904
                     /locus_tag="K0W68_002565"
     CDS             2314..2904
                     /locus_tag="K0W68_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBI3074094.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINGIC
                     EAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(3201..4022)
                     /locus_tag="K0W68_002566"
     CDS             complement(3201..4022)
                     /locus_tag="K0W68_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBI3074095.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(4133..4429)
                     /locus_tag="K0W68_002567"
     CDS             complement(4133..4429)
                     /locus_tag="K0W68_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074096.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            4729..5106
                     /locus_tag="K0W68_002568"
                     /pseudo
     CDS             4729..5106
                     /locus_tag="K0W68_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5344..5502)
                     /locus_tag="K0W68_002569"
     CDS             complement(5344..5502)
                     /locus_tag="K0W68_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBI3074097.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
     gene            complement(5782..6501)
                     /locus_tag="K0W68_002570"
     CDS             complement(5782..6501)
                     /locus_tag="K0W68_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBI3074098.1"
                     /translation="MSARSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGSRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            complement(6498..6932)
                     /gene="psiB"
                     /locus_tag="K0W68_002571"
     CDS             complement(6498..6932)
                     /gene="psiB"
                     /locus_tag="K0W68_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBI3074099.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            complement(6987..8945)
                     /locus_tag="K0W68_002572"
     CDS             complement(6987..8945)
                     /locus_tag="K0W68_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBI3074100.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            complement(9009..9242)
                     /locus_tag="K0W68_002573"
     CDS             complement(9009..9242)
                     /locus_tag="K0W68_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HBI3074101.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            complement(9298..9819)
                     /locus_tag="K0W68_002574"
     CDS             complement(9298..9819)
                     /locus_tag="K0W68_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBI3074102.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDKGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            complement(10647..11210)
                     /locus_tag="K0W68_002575"
     CDS             complement(10647..11210)
                     /locus_tag="K0W68_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI3074103.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREVLPCGTFAYTRVPTMIIRLRA"
     gene            complement(11257..12618)
                     /locus_tag="K0W68_002576"
     CDS             complement(11257..12618)
                     /locus_tag="K0W68_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HBI3074104.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(12670..12900)
                     /locus_tag="K0W68_002577"
     CDS             complement(12670..12900)
                     /locus_tag="K0W68_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074105.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPTPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(13938..14129)
                     /locus_tag="K0W68_002578"
     CDS             complement(13938..14129)
                     /locus_tag="K0W68_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074106.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(14126..14548)
                     /locus_tag="K0W68_002579"
     CDS             complement(14126..14548)
                     /locus_tag="K0W68_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBI3074107.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(14595..15020)
                     /locus_tag="K0W68_002580"
     CDS             complement(14595..15020)
                     /locus_tag="K0W68_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBI3074108.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(15438..16214)
                     /locus_tag="K0W68_002581"
     CDS             complement(15438..16214)
                     /locus_tag="K0W68_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074109.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(16265..16699)
                     /locus_tag="K0W68_002582"
     CDS             complement(16265..16699)
                     /locus_tag="K0W68_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBI3074110.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(16713..16934)
                     /locus_tag="K0W68_002583"
     CDS             complement(16713..16934)
                     /locus_tag="K0W68_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074111.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(16935..17618)
                     /locus_tag="K0W68_002584"
     CDS             complement(16935..17618)
                     /locus_tag="K0W68_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBI3074112.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(17695..17988)
                     /locus_tag="K0W68_002585"
     CDS             complement(17695..17988)
                     /locus_tag="K0W68_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074113.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            18135..19097
                     /gene="parM/stbA"
                     /locus_tag="K0W68_002586"
     CDS             18135..19097
                     /gene="parM/stbA"
                     /locus_tag="K0W68_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBI3074114.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            19100..19450
                     /locus_tag="K0W68_002587"
     CDS             19100..19450
                     /locus_tag="K0W68_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBI3074115.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(19573..19854)
                     /locus_tag="K0W68_002588"
     CDS             complement(19573..19854)
                     /locus_tag="K0W68_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074116.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(19982..20216)
                     /locus_tag="K0W68_002589"
                     /pseudo
     CDS             complement(19982..20216)
                     /locus_tag="K0W68_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421253.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(21152..>21644)
                     /locus_tag="K0W68_002590"
     CDS             complement(21152..>21644)
                     /locus_tag="K0W68_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074117.1"
                     /translation="GLFILSGENKIDEECFINIVQTAVAINNIGRESNGYILVGVSDT
                     KATADRVKTLYGVTPIECNGYYINGIDHEAVIQSKNIDNYFLFIKQKIESFNFNEALK
                     QQILKDISLCSYEGLHVLKIEIKSVGEVCHFENRYYIRQGTATHLITDGEAISSLHRL
                     YYS"
BASE COUNT         5296 a         5884 c         5220 g         5244 t
ORIGIN      
        1 acatcgtttt atttcctgtc agaggccaac gacgctcata cccacagcaa taaatacaga
       61 gatgatggca aagccgacca ggaacttgac gtttttggtc atcatgtaca tgaccgcacc
      121 gaccagcacc tcagccagaa caacccattt aacgatactg gagtccttac cgaaggtcgc
      181 cttaaccgta tcgttacctt tagccatcag gtcgccttta ccagcagcca tagctaactc
      241 agggaacaac atcatcagaa cagcaaccgg gatcactgca cgaaccaggc gaagcatatt
      301 cagacgagtg aatttagaaa aaaacgattt attttcaacg ggcgcagaag caccctgaac
      361 acttaaaaca gtattcatat gattcctctg attaatattg acagatgaaa actaaagttc
      421 tttaaatgtt gactcgttct cttcgatcac ctccctgaat atttcttcat tataaaaatc
      481 tggataacgt tgcaaatgat cctgcaacct caccaatgct tcgtttgttt tcgttcttcc
      541 actacgctct ctggctccaa gtaataattt attcgtttcc tcatcaaggg taagtaaaac
      601 ttttctactt ggcgcagatc ttaaagctcc tttaatcacc ctccctccca taaataaaag
      661 agatttagtc aaaatattca cataagacgc acacaaaaat aaacaacaaa atagtaaatt
      721 acgttaacaa aaaccggcac ttttagtgcc gggaatgctt acatttcttt acagtgaagg
      781 ctgttaatat tactagcgaa gtattgatat agataaaata acacatcaga gattatagca
      841 tatcgcaacc actcatccct gtctgagaaa ccatagtgtt ttttaaaatt cataaatatt
      901 tttttagatt ggtcaacaga gatatccatt ctctgagaag aatactcatg actataaccc
      961 aaaagatacg gtattatctt attaaagcga tatttgtcag gttcacaacc aaactccttg
     1021 ccctttataa aaaagccact aactacgtat ctttcaaaga atctccagac aaacaaattc
     1081 aagttattca cttccgtact ttcaaccacc acatcccatg atacatcatt caatattaca
     1141 ccattaataa tactcactcc attcgaggac gagaaagcat caacttcagc agtatgaaat
     1201 aaaatttgcg tatcaatatc aataccagaa aaccatattt cagaatcact attctgacac
     1261 tttattaagt gtaaaaaaac agagtttgag tctatgaatt ttcctgactc tgtgcggaca
     1321 cagactggat attttgcgga taaaataatt tccggcaggc ctgattgacg tatgagtgct
     1381 ctttctctag tatccatcgc acacatagga acctcctcaa aggattctat ggacagtcga
     1441 tgcagggagg gacaagctcc ctgcatcggc gattttcaag ttcaaatttt aacgtggcat
     1501 taaccacgta attaatatac taattattct gattattgtc atcagtttga accattaaaa
     1561 agtctgatat ttgaacaaag tcaaaattta tttcccttta ttcatcatca ttttttggaa
     1621 agaacctttc catctcagcc gacaccttct ctctgatatc ttcaaccata ttggcatatt
     1681 caaactttgg atttcctgag acatgaggac tgagagactc aatacccaaa attttcgcta
     1741 ctgatgactg tgtttttaca acgcattcaa gaagtagttt attaaactcg gcctgattaa
     1801 atgcagactc tttgcgctcc atctgagcct catatacacg aagacccagt tcaagaagca
     1861 ttgaagccgt tgctgaaaaa ctgacatctt tttctctcgc cccctcctgt cgacgttttt
     1921 caataatcgc gtttattttt tcataagcat cattgctgat atacagattc accttagcca
     1981 ttagatatta ccttttttaa acataaaaca aagttaattt cgaaacacta gaattcatag
     2041 tgcaaacagg gacgcaccgc tagcagcgcc cctgacggta tcctataaaa aaacacaccg
     2101 cgccgctagc agcaccccta atataaaata atgtttttta taaaaatagt cagtaccacc
     2161 cctacaaagc ggtgtcggcg cgttgctgta gctgcgttaa cgacgctgct ttaaaaaaat
     2221 cagatttaaa caatataaat ccacaaatac aactcaatga tattaaagat aaatcagcaa
     2281 aaacttgttt ttgcgtagtg tgtggtgctt ttggtggtga gaaccaccaa cctgttgagc
     2341 ctttttgtgg agtgggttaa attatttacg gataaagtca ccagaggtgg aaaaatgaaa
     2401 aaatggatgt tagcaatctg cctgatgttt ataaatggga tctgcgaagc cgccgattgc
     2461 tttgatcttg caggtcggga ttacaaaata gacccggatt tactgagagc gatatcatgg
     2521 aaagaatccc gttaccgggt taatgccatc ggtattaatc cggtaacggg atatggcagc
     2581 ggactgatgc aggtagattc ccagcatttt aacgaactgg cccgctatgg aattaagccg
     2641 gaacatctga caacagatcc ctgtatgaac atttataccg gtgcttatta tctggcaata
     2701 gcctttaaaa aatggggcgt ctcctgggag gccgttggtg catacaatgc cggattcagg
     2761 aagaccgaac gccagaacca gagacgtctt gcctacgcat cagaggttta ccggatttat
     2821 acctggataa agagcagtaa aggcatccgg gttccgacca cgaagaaatc actttcccaa
     2881 attaacagtg tgcagaaaaa ttaatattgt atacattgtc aaataccggt tatgccgcga
     2941 cagcggcatg gaaggccaca cacaaaaata tcatatgagt gataatgctg actggccgaa
     3001 gccccgcaaa gcgggatccc atcttatata tatccggtaa ctgcgcagcc tcctgccaga
     3061 ccatttttcg cgggcacaat atttacagac taccgaaaag ccagttgatg aaattgaggc
     3121 atatcagacg acacagcaat gccgcaggat gataatataa gtaaagcccc gaaaaatttt
     3181 tcggggcttt acttatatta tcatgacttc cactccctga actgttcggc aatcatccat
     3241 aatgctttgt tgattcggat atcaccgtca atgcctgtaa tggctcttgt tctggtattt
     3301 ttaccggagg cacttcgccc cgataatcca ccttttatca tattctcctg aacccgctgc
     3361 caggttgtcc agagatcgtt ctgtttatcc tcccaacggc ggggagtaat tatttgttct
     3421 ggcgtcactg gcgttttatt ttcatcttcg tacctggcca tcaatgcagc tctgccaaat
     3481 aaatgttgtt cgtcactgtt aagatgtatc tctttcattg tctccatatt ttcagtgact
     3541 ttatcaaaga caccgagcac ttcatacgct ccctcaataa cctgcccgac aatatcacct
     3601 ttatgtggaa cgcggatttc gccaaaatta ttcccacaca ccaggccatt tgtgcagaca
     3661 aaacgaaaaa ttcctgggat catctgataa ctggatgaac catcatgtga attaagcagg
     3721 ataatttcag gaacttcttt cccgttaata tgcccttccc tgcgaagacg taacatatgt
     3781 ttactgtatt cgcggcgtcc caaatcacga acccgactct gacaggcaaa gaatggctgg
     3841 aaaccttcat cacgtaactt attgatgata ttgatggttg ggatatatgt ataacgctca
     3901 ctccgggatt catgtttatc accagaaaag acagatggaa caaactgcat taattcgtca
     3961 tccgttaacg ggcgctcacg gcgaatagaa ttatatcgac caaaacggga agctaatcgc
     4021 ataacagtaa ctcctttatg aaggcataaa attaataaaa gttttttcgc ttaatatcat
     4081 cccccctcgg ttatcgttaa caaaaagaca acaattccga ggggaaggac catcaaatca
     4141 ggtcagttcg ggactgacca acctgccgag tgaaaagcag taacagcgcg atcaactcat
     4201 cagcgttttt tctgacaaga ggagtatgat tctccaggct atccagtaag tcatgcagct
     4261 cattattgac ctggtggagc accttaaaga gtgcaccaga atcccggcag ctctgatatg
     4321 caagatttgc ctgaacagca cgattctgca tgatcccgat ttgtgcgatt gcgtctttct
     4381 gctgatcgcc tgttggctga taagtggtgt taaaagaaaa tgcgctcata attcctcctg
     4441 tatctgatgt tttcagcccc catcatctgt tgagtttcgg gagccgcggt tttgacgttt
     4501 tgcggggacc tgccttttgt tccctccggt attcaccatc acctgtttga gaaccttcgc
     4561 cgcaaggggc atcactgcca cggcctgacg cacctgtcct gggcagaacg caaaattttt
     4621 taccgggttt tgggtaaaaa attttgtggg ctgttcattt accctggaca gtcagtgcgg
     4681 caggttgtgg ctgtctatgc ccccggcgac aggggcttaa acaggtgaat ggcaccggac
     4741 agaacaacgg gcagggaccc gccgcaacga cgtgaagcgc ggttcgcgaa acggcgttgc
     4801 tccttacatc ccggcgtctt ttgccgggat gccgggagcg caggccacgg cgcgacaagg
     4861 gtggcgcgac cgttccggtc gctgcaaacc cgggtttttg cgtcagggat ggaagcccgc
     4921 cagggcggag acagcttcgc tggctccgtt cacgacagcc cgccccgcca gggggacgcc
     4981 cggaaaaggc ataagcagga gaacaacaaa acagacaata atcagggcag cagtaatcag
     5041 agtcagagca gtaatcagag tcagagcagg aggaaaaaga gaataatcaa agggaaggtg
     5101 atgtgatttc tggtaagtca taaacagaga aggcggggga aaagaacggg cgccgcaggc
     5161 gcacgttcat ccccgttccc ggagggaaac ggccttcctg gcagtctggt tgttcatgcc
     5221 cgtacaatgc cagcaaggag aaaggggcca ccggcgaacc agcagcccct ttataaaggc
     5281 gcttcagtgc agtcagacca gcatcagccc tgaaaaggcg ggcctgcgcc cgcctccagg
     5341 ttgctactta ccggattcgt aagccatgaa agccgccacc tccctgtgtc cgtctctgta
     5401 acgaatctcg cacagcgatt ttcgtgtcag ataagtgaat atcaacagtg tgagacacac
     5461 gatcaacaca caccagacaa gggaacttcg tggtagtttc atggccttct tctccttgcg
     5521 caaagcgcgg taagaggcta tcctgatgtg gactagacat agggatgcct cgtggtggtt
     5581 aatgaaaatt aacttactac ggggctatct tctttctgcc acacaacacg gtaacaaacc
     5641 accttcacgt catgaggcag aaagcctcaa gcgccgggca catcatagcc catatccctg
     5701 cacgctgacc acactcactt ttcctgaaaa taacccgctc attcaggctg tttacgggta
     5761 atctgtgtga ttgttgccgc atcacgccgc ctcccggagt ttgttcggca ccatccaccg
     5821 ctccatctca cggacagcgt gaccggcctc tcttgctgca agactggctt ccgcaataac
     5881 ctgccagaac ggcatatcct gccactgcca gcgttccatt ccggccagtg acggaaaacg
     5941 ctcaccccac tgccagaaca gggtttccag gtcatgctct gccacccggt cactccagcg
     6001 tgcataccag cttccgactg tttccggcga atggaatgcc agttcgattt cagcctgagc
     6061 gaccagcgcc tggtacgcac gggctttctg ctctgcctcc ctcgcctgct ggcggctgta
     6121 cttctgcatg accagcgcgg atttatggcg actgcgttcc ctgacacgaa accgcaccga
     6181 cgggaacaag cgttctgctg catcgcctgg aagaggaagg aggcagacct cgccctgtgt
     6241 ttcaatcagc acatcgatgg catacagcca ctgaagacgg ctgccgtgca gttcttctgc
     6301 ggtcagggac ggcatgaaaa atcgcaggtc agacagcgat atcctgcggc ttccgttcag
     6361 acagcggaaa aacgcccccg catacggata ttccgccagc gtgtttccct ggtggcggcg
     6421 tttttctgtc tccgccaccg cccgccatgc ggcctgttgc tctgcgctga ggggaaccag
     6481 tgcctgtgaa cgtgcgctca tgcctgacct ccttccgcca tcaggatatg gatgatgtca
     6541 ctgacgctgt acccctgcgc gtccagtgac gccgccagtg ccagtgaatg gctgacggct
     6601 tccggtgcaa accgccgctg tacctgaacc accgcaaacg gctcaccacc ggcattcacc
     6661 agaaccagca cccagacctg agagacatcg cccggtgaac ataacgccag gtgaaaacgt
     6721 tcgtgggcag gcgtgaaacg gacctggacg gggaaaaatc cgccaaactc gctgccgtac
     6781 tcgccgttca tcgtccagtt gtccggcgca tccagttccc gcatcacggc gtgtgtcagc
     6841 tcacgacgtt catcacttcc ggcagcccgg atatcttcat attcctgtgc gctcatggtc
     6901 tgtaatacat tcagggtcag ttcagttttc atcacggtat ctcccttgct gccgccccgt
     6961 ctccggcggg gcggtgtggt tgttcatcag gcggcatcag ccaggttatc ggtgcgttca
     7021 gtggtatctg tctgtggatg tggtgcacac atccagccag gcacccagcg gttgtctttc
     7081 atatggtgct ctgcatgttc agccgcatca cctttcttca tcttctccgc gtcccgtgcg
     7141 gcaccggaca gccctgcctc attcagggct gcgataatct gcggcttttt caggtgtccg
     7201 aagaagtttg cttttgtcgg ctgccaccag tcgcgcatgt gaaagccgat ggcgctttcc
     7261 agcgtgtcaa gcggactgcg tgacgtgtga ccacactcac gggtctggac accgttaagg
     7321 ctgcatgcgg tgcagaaact gagcagggat aacagcacct cctgactgag tgacaggaat
     7381 gtcgtcatgt cccgtgacca tccctccggt aacagggcag caagacgggc tttttctgcc
     7441 atcagcgcca tgaatgcggc accttccttc cccgatggcg catcgctggt cagcgaataa
     7501 tgtttacatt ccaggctgat ttgtgctggt ttactgtacg ctccgctgcc aaacacattc
     7561 aggcagagcg tccatgccag cagtgtcagg gatttgtccg gttgctgcat cagtgctgcc
     7621 tggactgcca gcgtgcgttc ggaagacatt ttcgtcagca gtggcagact gatttcctcc
     7681 actgacgctt tctcctgcac ctgttccgta cggtcagcgt cgtcagccac gtcatcctca
     7741 ctgcgcaact gcacaccacg ctggacacac acgttgccat aacgccagga caccaccact
     7801 ccacctccgg cacgcatctc cggcgtccac gccctgacct tcgccatgca gcgcatcagc
     7861 ttcatttctg cttccagcag gtcagattcc tcacactggt tttccagcgc gtcgtaacgc
     7921 gtcatcagtt cgttcagacg ttcttcttcc gcctccgtca gcaccgcttc cggctccggc
     7981 agacagcggt atgttccggc atcctcacgg cactcaccga caggctccat gcgtccggcg
     8041 caccattccc agccttcggc ttcccgaagg aactctgcga cagcctggag tttttccagc
     8101 agggcggcat cgagcgccac gcagtccaca tagccacctt cgtcgtcgct gaacaaatcg
     8161 gtgcgcagtt cgtctggcga gaaggcatca gcccccacga agcggaattt actgttcccc
     8221 gccaccgcca cttcactttc ggtcaccaga cgacgaatgg tctgtacttc cggtttaccg
     8281 ccccatcccg actgacaggc ggcttcaaac acctgcacct gacgcgcggt gtcgttctcc
     8341 agcgccagcg cctgacagtg ttcggtggtg atgcggtctt ctgccagcgc atcgaggatg
     8401 acaggcgcaa ggtcagccag tttcagcatt cgctgaacgt ggcggggtga atagcccagc
     8461 aaatcaccga tttgtgcagg tgttttgcct tcctgcgcca ttgcgcggaa tccggcaatc
     8521 tgttcggcag ggtgcatatc ccgacgctga ccgttttcag tcattgatgc agccgtcgcc
     8581 agctcctgcg gaataacctt cacgcgtaca ggccagtcag ccggaaggat gccacgctct
     8641 gccagcatgt tgagtgctgc cagtcggcga ccacctgcgg cgacaccgtg gcggtcacca
     8701 ggcagggcat gaacaaccag attctgcaac aggccaacgc ccttaatgga ctccgccagt
     8761 tcgctgacgg actctgcgga atacggcacc gtgcgcacat tcagcggtga tttaatcagc
     8821 gaggccagcg acacgctcac ttcctccgtc tgctccagca gggcagacag gactgtttcc
     8881 tgcgtttttg caggtttacg gctggattta cgctccgttt ttgtcttaga ctctgtaact
     8941 gacatatatt tctcctttga ccgggatggt tgtatgtctg ccggacgcgt tcccgtccgg
     9001 cacctctctt acttaagaat gccgaaattc cggatgacat ggttcatcca gtacccggca
     9061 ccgtcctcaa aattccaggt acgccagacc aggccactgt tattgcggac aaccaggcga
     9121 aaatgcgttc cctgatcatc ctcgataaag acattccggt actgtgcggc aacggcttca
     9181 gcctgttcgc gggtaaagga gccgtcaggc agcccctgaa atattctgaa atattcgctc
     9241 atcctcatat ctccggtgat gccccgcact ggacggggcg gttgtcactg tctttcgtta
     9301 gaacggaatg tcgtcatcga agtcatacgc gggttcagac ggctgctgag gctccggctg
     9361 cgcggcctta cgtccacggc cttttgtttt cgcgctccct tttttcgtcg tgtcagcact
     9421 ctgtggctga ccattctgct gaggctgcgg ttgcgcctga gcgttctgct gtggtgcact
     9481 ccccagcatc tgcatggtgc ccgtggtttt cacaaggatt tcagtgacgt aacgggtaat
     9541 acctttatct tcccagctac gggttcggag ttgcccctcg atgtagacct gcgcgccctt
     9601 gcgcagatat tcacctgcca cttccgcgag cttgccgaac agcaccacgc gatgccattc
     9661 tgtctgctcc cgcatctccc ccgtctgttt gtcacgccag ctttctgacg tggccacctg
     9721 caggtttgcc actgcgcccc cgttggggat gtaacggact tccggatctt ttcccagacg
     9781 accgacaaga atgaccttgt taatgccacg aactgccatg atgtgtctcc ttctgttgat
     9841 gttttcagcc cccatcatct gttgagtttc gggagccgcg gttttgacgt tttgcgggga
     9901 cctgctcttt gttccctccg gtattcacca tcacctgttt gagaaccttc gccgcatggg
     9961 gcatcactgc cacggcctga cgcacctgtc ctgggcagaa cgcaaaattt tttaccgggt
    10021 tttgggtaaa aaattttgtg ggctgttcat ttaccctgga caggtgcggc aggttgtggc
    10081 tgtctatgcc cccggcgaca ggggcttaaa caggtgaatg gcaccggaca gaacaacggg
    10141 cagggacccg ccgcaacgac gtgaagcgcg gtttgcgaaa cggcgttgct ccttacatcc
    10201 cggcgtcttt tgccgggatg ccgggagcgc aggccacggc gcgacaaggg tggcgcgacc
    10261 gttccggtcg ctgcaaaccc gggtttttgc gtcagggatg gaagcccgcc agggcggaga
    10321 cagctccgct ggctccgttc acgacagccc gccccgccag ggggacgccc ggaaaaggca
    10381 taagcaggag aacaacaaaa cagacagtaa tcagagcagc agaaaaaaga agaacagtag
    10441 cagtagcagt agcagtagca gtagcaggaa caacaacatc atcagtaaca ggcaaatgac
    10501 aggtctgaaa agacagaagc ccacagcaga acgtccgcgt aatgaaaggg gcggcgcgta
    10561 agcgcggccc ctttcagcgc caccggcgcg cggtcttttc ccggcgcggg cagacgggca
    10621 tttatcctcc cgcctgcccg gattcatcag gcacgcagac gaataatcat cgtcggaaca
    10681 cgggtataag caaatgtgcc gcacggcagc acctcgcgga catcagaaaa cggtaacagc
    10741 ttctcctgct ggcgcggccc gttcagacag acggcaacca gaacgccccc cggacgcaac
    10801 agggaaaaag cccgcaggat atgccggata tcctgcccgt gactgaacgg cggattcata
    10861 atgatccggc tgtaatactg caccggctgc cattccatga agtcgccaca ctgtaccctg
    10921 acaccgttaa agttttcccg cagataccgg accagcccgc tgttaatctc caccgcatca
    10981 cacatggcct ccggcgcggt atcccgaatc gcccgcagta tggccccggt gccggcagag
    11041 ggttccagaa tgtgatcacg gttgctgatt tccgccagcg tcaccaggcg atcacagacc
    11101 ggggcaggag tcacaaaaag ctgagattca gcccggattt cagcaaccgg cgcggcgtta
    11161 tccagccttg cgcgcatcag ccggatacgt tctcttaact gactgtgcat gtcattatct
    11221 ccgtcatccc ccggggattt cccgggggga tataccttat ttctgcggga tttccgtgat
    11281 tttcatgtcg gtgatataca cattcaccag acggaaatta ttgtccattg tgcggcggta
    11341 gcggtatgcc ccgtggtctt ctgcttcttc cacactgcgc acggccttac agtcccgggg
    11401 cagggcggcc cactgcgcct ttgtcatttc ccggaaacct tcccccggat agttgactac
    11461 cggcggacgc ttcgcggcct gactggcgac ggcagcctct tctgccgatg gtgctttgta
    11521 gtccgtgatg cgatcgggcg tcactttcat cgtgccgctg tacccgagaa aactgtaatt
    11581 tggcgttgtg actgaactca ccgccccgtt gcttttgttc acgcggatga tagtcagcca
    11641 ctcgccccgg ctgaaaacct gtccgcccgg ctcaaaatcc tgtgtccggg taaccacgcc
    11701 gccgctttcg tccagcatgg cacgctcata gatcaggcgg ttctgatagt ggttaaccca
    11761 gcgttgctga tgctgaatct gccgttcatg acagcggatc gcaatttcgc gggcctgctc
    11821 cgtggtgatg atgtcatcat ccagggccga ccataacgac cggctccctt catactggct
    11881 tttttctgcc gggcgcggat acgtatccag cgggaaacag gcgctgatat ggtcatgact
    11941 gctgatgagt tttgccatat tcagatccag cgattcagcc cgccacatcg tcagatattt
    12001 ctgcgactgt gcgatggtct tttcagcctt gcgcaaatcc gcttcgattt ttttgatacg
    12061 acgccagcga acgtccgggc gttctttata cttcgcgtga agcagtgccg accgcgcccg
    12121 ctcctcccag tattccgcac gttcaaagag catcacggca cgtttcatgc cgttttcaat
    12181 acgctgcgca tcacgacggg cgcggcgttc gctgtgatgc cccacaagaa tgggctgacc
    12241 gggcgggatc atcgcggcca gtctttccac ttcatcaagt gcctgtgcgg attcactggc
    12301 ccgctttccg ctgtatccgg taaaccgctc cgcccgtgct tcctgacgtt cagcgagcgt
    12361 gctgtcttca tcctcaatct ctccggcaag tgagaggagc acgtcttccc ggcccggtgt
    12421 ccaggccggc gcgacaaaca gcgcctgttt gggtgcccag cgaaaaccgg catcatgcac
    12481 ttttttatac gtctccgggt caagacgtga cgcggcatac aggcgcagtt tgttgtcatc
    12541 cggtgagtag gtcgcgcggt acatctgcac agggctggcg gcttccgggg cggtcatggt
    12601 gattgctgct aatgtcataa aatgttctcc ttccgtctgt tgccgcccct cgcggggcgg
    12661 ctcacctcat cagttaaaag cgcgtttcag ttcgagtgtg cggagcacct gcccgaaagc
    12721 cgtgtttttc atggcgggga tgtgtacacc acgcccccgg tgaaaactcc gggctatgtg
    12781 tcgcccgaag gctttcaaca cggggtcaat ttccggcgtg gggatgtctt cgcgtctgaa
    12841 acggggatga cggcggggct ttacagccgc tgcggaaggt gaaacaatgc ttacagtcat
    12901 aaatggattc cttatgacaa attggttaac aatgccgtca aaattggtcg cacaattctt
    12961 acggcgtgga ttaagcaggt gcctgacctg cttttttcat atctgcatct gacgctttcc
    13021 ggtgttttcc tccttctgtt cctgcgttct cattacctgt cagccggatg actgacagaa
    13081 caaataaatg cgtattgccg gagcctgaaa taaatcctga tttttaaaga acatcacccg
    13141 ttgagtttcg ggagccgcgg ttttgacgtt tcgcgggaac ctgctgctgt attcactgtg
    13201 gtatgaactt cacccgtttg agaaccttcg ccgcaagggg catcactgcc acggcctgac
    13261 gcacctgtcc tgggcagaac gcaaaatttt ttaccgggtt ttgggtaaaa aattttgtgg
    13321 gctgttcatt taccctggac agtcagtgcg gcaggttgtg gctgtatcag cccccggcga
    13381 cagggactca tacgggtgtg tggcacccgg caggcaatga gcaggggccc gccgcaacga
    13441 cggagagcgc gtgcgcgaaa cggcgttgcc ccttacatcc cggcggcctt ttgccgggat
    13501 gccgggagcg caggccacgg cgcgacagag gcggcgcgac cggaacggtc gctgcaagcc
    13561 aggggttttc acgcatcgga ccgtagcggc agcccgaaac cgcgccagcg ggttcggcgg
    13621 agactggcag gtgcgccagc agctgcctgt cggagtcggg cctgacgacc ggatgccgcc
    13681 aggcagccgg gagccgaagg tgcgcccggc agacagaaca acactgcagt agtcagaatc
    13741 agagcagtag ataaaggaac aacaacatca ggaacagttc cataacataa gaaagaaaca
    13801 ctcctgagtc agacacatca cagacgggaa gggtaacggg cgacgcgcca gcgtggcccg
    13861 cttcccttcc ggcaggcgtc agcctgccag gggttttcgc gcgggctgac aggcggcctc
    13921 caccgcctgc ccctgtatca cctgtttatg ctgccctgaa ggatgtagtg accgccgagg
    13981 aatacagcct gctgattcgc cacaaaatcg cagatttcat caacaatctg cgcagtggtg
    14041 agcggtttct gccccatttc acgacgccgc gcattaatca cggctttgta attgcgcaga
    14101 atttcgcggg tttctgtgct gatattcatg acatgtgctc cccgtccagc gcctgtaaca
    14161 caacgtccat tgcttcgcgt tcttccctga cgaatgcgtc accgtcgccc ccgttctcgc
    14221 tcccgacggc atacaggcgc ttcatttcac tgcctgccag cgccatcact ttttccagca
    14281 tcaccgccgg aactgtcacc tgacggctgg cgcgcttttc ttccatcagc tcacgaacaa
    14341 cgtcgtgaac aatgctgaca tccgcaccat gcgcaaaggc atcgtccagt cgctgcatga
    14401 cggtttccag ctcatcgtct gtcagactcc agtcctgggc tatgtgacgc acatccccac
    14461 gggtcagcac cacggcaaaa acgcattcac tgtccggcac gtctgtgttc agcacttcgc
    14521 ggataatttc tttaacagta cagtacatgt tctcgtctcc tttacagggg cggcattgcc
    14581 gccccgtccg ttaatcagtc cagtgccgcg taaatcgcgt tgcattcggg gtgaaattcg
    14641 atgtgacgcc acaactgcgc atcgcgcatc cggtaaagct gggtcagtcc tgcatccccg
    14701 ctgtcgtggt acagccacaa ctggcggtta atcacaaggg aagtcagaat gatgcctgcg
    14761 gcatcagcac tcacggtacg gtcaaaccag tttgcaccgt tcaccatgtg gaaacggtcg
    14821 ccatccggca tcatgtaacc gccaccgtcc ggcaggcgga tgaagtgcca gaagcctccc
    14881 tgatattccg gcatcatttt ttgcgccagc gcgtacacat acgcctcggc atacagaaaa
    14941 tcgttgccaa acagatacgg tagaaaggag agacggtcgc actcttcaac gttcagagtg
    15001 acaggtttcg catattgcat gatgttttcc tccgttgttc acatattccg cgacaccggg
    15061 cggtgccgtg cctccataaa agccgggcga taaggcgggg atgcacaggg cgcaagggaa
    15121 gccgcagcct gacgcacacg gctggcggga gttggagggt gagaccgcga aatctgagcg
    15181 ggtgaacgac cggaaaatca cggcagacgg gaaggaggga ccgggtgacc gcagcggcga
    15241 agcgtaccct tgcatccctg ccagcccggc taagatggag gcacggcaca gcccgcacac
    15301 gacgctgagg ccacataact gaaaaaccac aaggccatgc cctgcatggc cgggtaaagc
    15361 ggagccggcg gcgacttcct ttgtgctctg tccggggacc ggcaggaaaa aggcggggat
    15421 ttaccccgcc aacgtcatca tttccggatt tttgccaggg catccgccat gctgcggatt
    15481 tcggcccggg cttcccggac cacctcccgc agaacaccac ggtcaaacca gcggcggggc
    15541 ggttcatccg gtatacgcag cacatcgcag gccagcccac tggacaggat atgctcaata
    15601 caatcgctga ccaggtacat ttccaccgac gaaaaacgtg ctccccggtt cacgatggca
    15661 tcgagcacca cgggcaggtt ttcgtcatct tcatcgagcc agcaggggtg atcgctttcc
    15721 ttcacaacca gacgaaagcg gcgcgtctgg cgagtcagca acgtctgacc ggtggtggtc
    15781 agcgccgccc ggatacgccg cgcatcttct gccacccggc aactgacact gcgtaccgca
    15841 tcgcgcaccg ccgcaagtac agccctgatt tcgtcttcgg tcagccattc tccggcatac
    15901 gggaaagaca cctgcgttcc gtctgccgga agtaccggca gcgactgcca ctgactgccg
    15961 gacagtacca gttccgcccc ggttccggtc agtttctgaa tggcggaggc caggagcggc
    16021 ggcagtacgc cgccgtgacg gttgataagt aacgtcgcca gccggggcgc atccagcaga
    16081 acataaatgc tgatccagcc cggatacttc gggttacgct ggtcaacatc cgtctcttcc
    16141 ggccagccct gcgccagcag caggttgcgg gcgtgacgac aaatcagtgc gttcagcgtg
    16201 ctgttttcgt tcatgattaa gcgttctccc tgtatcgccg cccgcagggc aggcggcgat
    16261 aacatcagtc gttttcgtgt gtgttgtttt tcagcagggt gcgggcctga ttaaccacct
    16321 cctgacggcg ggtgacgcat tccgggacgg cgaggccatg atcccgggcg gcccattcac
    16381 gtttccacaa tgcctgttca gcggtctgaa tcagggacgc cagcagttca gcagggacgg
    16441 tcaccaggcg gttttcgctc tcgttgcgga taatctccat caccgcagcg gaagaaatac
    16501 cggactcaat ccgctggtct tccgggatgt cctccaggcg tgccaggacg gtcctgattt
    16561 catgaccggt cagagaaatt tccataccgt cagccagtgc ctgaatctct tccggggacc
    16621 agataaccag catcagcggc atgtctcccg gatactttac tgccagctcc cggcatagcg
    16681 tttcgcatgt tccgtacatt ctcgtctcct tctcaatctt taaagcacgc ttccagttcc
    16741 agaatttcgg tatatgcctc catcaggagg cggtttgcgc gaaacagcgt ctgacgcacc
    16801 agacgttccg gcagggtgtc ggcattaaag ctgtaccgct gctccatctc attcatggtt
    16861 gtacgcaggt cgcacatggc attggccacc tccgccactt cggcgcgcag tttttcgtgt
    16921 cctgcatagt tcatttacgc agcctccggc ataaaccagt catcattcgc ggccccctgc
    16981 tgcatggccc gttgcacggc ggcaaggcgt tgctgcccgg cacggtgata ctgctcaagc
    17041 agctcgatac cgatataccg gcgtccggac tggagggcgg cgacgcaggt tgagccgctg
    17101 cctgcaaacg ggtccagcac aattgcgttc gggtgtgtga agctctcaat cagcggttgc
    17161 aggctggtaa cgggcttttc cgtcgggtga tggcgattgc ccgaatattt ccagcccagc
    17221 acgtccggca gcgggttttg tggcagacgt ggacggcctt ttgccaggat gtaggcacat
    17281 tcgtggcgat agcccacata tgcggccttc gatgtgtagt ttttggtgaa gaccaggtga
    17341 ccaacaacgc tgaatcccgc atttttccag gcggccataa agcgatcgac gcggttccag
    17401 ccatagaagc tcaccattaa cgcgtctttt ttgagtacgc ggtacatttc attacaggct
    17461 ggttgcagcc attcatcggt tttatcgccg gcgatggtgc gcccggaacg gtcacggaaa
    17521 ccgacgagat acggcgggtc ggtgaggatg aaatcgacag cattgcccgg aaacgtggcc
    17581 ataacacgca cgcagtcacc caggataaaa cgggacataa cagcactcct tctcttacag
    17641 agggcaccgg gagggcaccc ctgtggtgcc cgttccccgc tgcctgtggg ctgttcaccg
    17701 cgtccggtaa cgggtcaggc cgaccgtgga ataccgcagc cgtcgtcttt ttgacggcga
    17761 ggatatgccg cgaaagcggc ttgaagggcg ttcatcagaa aagggcgacg ggctggcgta
    17821 cactgtcacc aacgccagcc gggagcggta aaagcgcccg tcccgtgccc ggccggacgc
    17881 gggacactga ccacaggcag cgttcctgcc gcgccgcgtc agccggcgca gtaaaaagag
    17941 gagcccggcg acatcttttg ttacccgcca aacaaaaccc aaaaacaacc catacccaac
    18001 ccaataaaac accaaaacaa gacaaataat cattgattga tggttgaaat ggggtaaact
    18061 tgacaaacaa acccacttaa aacccaaaac atacccaaac acacaccaaa aaaacaccat
    18121 aaggagtttt ataaatgttg gtattcattg atgacggttc aacaaacatc aaactacagt
    18181 ggcaggaaag cgacggaaca attaaacagc acattagccc gaacagcttc aaacgcgagt
    18241 gggcagtctc ttttggtgat aaaaaggtct ttaactacac actgaacggc gaacagtatt
    18301 catttgatcc aatcagcccg gatgctgtag tcacaaccaa tatcgcatgg caatacagcg
    18361 acgttaatgt cgttgcagtg catcacgcct tactgaccag tggtctgccg gtaagcgaag
    18421 tggatattgt ttgcacactt cctctgacag agtattacga cagaaataac caacccaata
    18481 cggaaaatat tgagcgtaag aaagcaaact tccggaaaaa aattacatta aatggcgggg
    18541 atacattcac aataaaagat gtaaaagtca tgcctgaatc tataccggca ggttatgaag
    18601 ttctacaaga actggatgag ttagattctt tattaattat agatctcggg ggcaccacat
    18661 tagatatttc tcaggtaatg gggaaattat cggggatcag taaaatatac ggagactcat
    18721 ctcttggtgt ctctctggtt acatctgcag taaaagatgc cctttctctt gcgagaacaa
    18781 aaggaagtag ctatcttgct gacgatataa tcattcacag aaaagataat aactatctga
    18841 agcaacgaat taatgatgag aacaaaatat caatagtcac cgaagcaatg aatgaagcac
    18901 ttcgtaaact tgagcaacgt gtattaaata cgctcaatga attttctggt tatactcatg
    18961 ttatggttat aggcggtggc gcagaattaa tatgcgatgc agtaaaaaaa cacacacaga
    19021 ttcgtgatga acgttttttc aaaaccaata actctcaata tgatttagtt aacggtatgt
    19081 atctcatagg taattaatga tggacaagcg cagaaccatt gccttcaaac taaatccaga
    19141 tgtaaatcaa acagataaaa ttgtttgtga tacactggac agtatcccgc aaggggaacg
    19201 aagccgcctt aaccgggccg cactgacggc aggtctggcc ttatacagac aagatccccg
    19261 gacccctttc cttttatgtg agctgctgac gaaagaaacc acattttcag atatcgtgaa
    19321 tatattgaga tcgctatttc caaaagagat ggccgatttt aattcttcaa tagtcactca
    19381 atcctcttca caacaagagc aaaaaagtga tgaagagacc aaaaaaaatg cgatgaagct
    19441 aataaattaa ttcaattatt attgagttcc ctttatccac tatcaggctg gataaaggga
    19501 actcaatcaa gttattttct taccagtcat tacataatcg ttattatgaa ataatcgttt
    19561 gcactgtctc tgttattcag gcaatttcaa taaaggcact tgctcacgct ctgtcatttt
    19621 ctgaaactct tcatgctgca tttcgatttt tcttctaaaa tcataacttg catacctgtt
    19681 ctttttatgt atatccagat ctatttttgt gacgagttca aaccacaatg atagcactcc
    19741 ccatgcagcg ttatgaaaca tttccgcaac agatttagag tctgtattaa ccgcttcagt
    19801 catgtagtcg ctaatacgtt ctgcagtgat tttcgttatt tcatcgtaat tcattttatc
    19861 ccctacaaga ctatttaatg gaatgaaaaa tatttggtct gataacaatg ctcctgaatt
    19921 aacacctgca ggctatgtta aagttgccct cacacacaat ctacctgttt tctcgtgtgt
    19981 gtcacaggcg caatacataa atagtctggc cattaagttc gtaacggatt tcgtactcgc
    20041 cagcaaaaat ccgtctgaca tccctggttt caaactgaaa caactgttca tcccatacgt
    20101 ggatgagtta acagaatgac cggcgcctgt gtcagggact acaccgtttt ggctgcagct
    20161 ggtttactga ccaacgccag aaaatcatat aactgcgtca atgtcagcgt atccagcgcg
    20221 actgtattat ttttctctgc aaccagaata ttgcattagg ttattaccag gtgttctaat
    20281 gtacaggtaa agacgttctt tataatctga aatctctact tttaatccct cagactttgc
    20341 ctgctgaaag ccctcgtcaa ggtgcgcttt tattaggtta atgtctggat gtcttgtatc
    20401 gggggcaatt ctgtattggt gtaacgatgg ggtggatgtt ccctgcaata agacctccag
    20461 atagagattg gcacaaccat tcccattatt tctaatagtt tattggtaat cacaaactgt
    20521 atatttagcc attatattga atcctgtgaa gtaaacggcg acggctcaaa attttatcaa
    20581 acctaagata attctaccat caaagttaat aatttgaatt tatatgacgg ctctatgggg
    20641 gggtaaaaat ccccgtgcag agaggcgatt ttcagagttg aaaacgtcgt cgttaagaac
    20701 accaaagtca gtaccaccaa cccggaaagt agcttcatgc accgggacaa cagcagtttt
    20761 ggtgtccgaa atcgtacaat tctgtacatc tacgcaagga aatgtacgga agtaagccaa
    20821 atggttatcg gacggtcgga acatttcgta cagcctttac gttcagtgag ttagctggcc
    20881 tagatgtaac cactcgttca actcaattct cttagcgtgg gaaaatttcc gttttccaag
    20941 cggccccctc aagcggaccc cttatagtac aataattttc gatatccaaa ctgcccccaa
    21001 ttacatctcc agtctctggc agcagagatc atatatttcc attgccgccg tagccacgat
    21061 attctggcta ttcacaaaaa caatttagcc ccaaagcgca gtaaaaaagt acactatgaa
    21121 ggtgtatgat accttcatag tgcttaagta attatgagta atataatctg tgtaaactac
    21181 taattgcttc accgtcagtg attaaatgcg tagctgttcc ttgtcttata taatacctat
    21241 tttcaaaatg acagacttct ccgacacttt ttatttcgat ttttaaaaca tgcagtcctt
    21301 catatgaaca taatgaaata tcttttagta tttgttgttt caaagcctca ttaaaattaa
    21361 atgattctat tttttgtttt atgaacagaa agtaattatc tatgtttttt gattgaatta
    21421 cagcttcatg atcaattcca ttgatataat agccattgca ctcaataggg gtaacaccat
    21481 ataaagtctt aactctgtct gccgtagctt tggtatcgga aacaccaact aaaatataac
    21541 cattagattc tctaccgatg ttatttatgg cgacagccgt ttggactata ttaatgaaac
    21601 attcttcgtc tattttattc tcgcctgaga gaataaataa tccc
//
LOCUS       DAETQN010000069        21643 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.069, whole genome shotgun sequence.
ACCESSION   DAETQN010000069 DAETQN010000000
VERSION     DAETQN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.069"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="K0W68_002591"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="K0W68_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBI3074118.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPRSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="K0W68_002592"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="K0W68_002592"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBI3074119.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="K0W68_002593"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="K0W68_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBI3074120.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="K0W68_002594"
     CDS             complement(3717..4511)
                     /locus_tag="K0W68_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBI3074121.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="K0W68_002595"
     CDS             4649..4990
                     /locus_tag="K0W68_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBI3074122.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="K0W68_002596"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="K0W68_002596"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBI3074123.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="K0W68_002597"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="K0W68_002597"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBI3074124.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="K0W68_002598"
     CDS             8906..10198
                     /locus_tag="K0W68_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI3074125.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="K0W68_002599"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="K0W68_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBI3074126.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="K0W68_002600"
     CDS             complement(10731..11189)
                     /locus_tag="K0W68_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBI3074127.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="K0W68_002601"
     CDS             complement(11186..12103)
                     /locus_tag="K0W68_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBI3074128.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="K0W68_002602"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="K0W68_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBI3074129.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="K0W68_002603"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="K0W68_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBI3074130.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="K0W68_002604"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="K0W68_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBI3074131.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="K0W68_002605"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="K0W68_002605"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBI3074132.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="K0W68_002606"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="K0W68_002606"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HBI3074133.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /locus_tag="K0W68_002607"
     CDS             16063..16749
                     /locus_tag="K0W68_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3074134.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /locus_tag="K0W68_002608"
     CDS             16746..19160
                     /locus_tag="K0W68_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3074135.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="K0W68_002609"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="K0W68_002609"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBI3074136.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..21483)
                     /gene="allS"
                     /locus_tag="K0W68_002610"
                     /pseudo
     CDS             complement(20557..21483)
                     /gene="allS"
                     /locus_tag="K0W68_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5015 a         5684 c         5799 g         5145 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccgcgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgaccaggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccaatg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccagtttt atcgaacacg atggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttt aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
    17581 cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    21181 caggttgttg atgacaatat tcacctagcg ttccacgcca tcattcacct gttgcagctc
    21241 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    21301 agccgctgtc aacgtcacgc tgcgagtcgt gcggaaaaac agcgccactc cggtattctc
    21361 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    21421 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    21481 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    21541 tcagtatttt tacatgataa ccctgttcaa tttgtggact aaatctagtt ttggaaaaat
    21601 attccaactt ttgtattgat gttgttctct taaggtttta gat
//
LOCUS       DAETQN010000070        21044 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.070, whole genome shotgun sequence.
ACCESSION   DAETQN010000070 DAETQN010000000
VERSION     DAETQN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.070"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..856
                     /gene="espL1"
                     /locus_tag="K0W68_002611"
                     /pseudo
     CDS             1..856
                     /gene="espL1"
                     /locus_tag="K0W68_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1028..1135
                     /locus_tag="K0W68_002612"
     CDS             1028..1135
                     /locus_tag="K0W68_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074137.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1188..1946)
                     /locus_tag="K0W68_002613"
     CDS             complement(1188..1946)
                     /locus_tag="K0W68_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBI3074138.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2233..3162
                     /gene="pfkB"
                     /locus_tag="K0W68_002614"
     CDS             2233..3162
                     /gene="pfkB"
                     /locus_tag="K0W68_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBI3074139.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3263..3553
                     /gene="ghoS"
                     /locus_tag="K0W68_002615"
     CDS             3263..3553
                     /gene="ghoS"
                     /locus_tag="K0W68_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBI3074140.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3659..4519
                     /locus_tag="K0W68_002616"
     CDS             3659..4519
                     /locus_tag="K0W68_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBI3074141.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4560..5096)
                     /locus_tag="K0W68_002617"
     CDS             complement(4560..5096)
                     /locus_tag="K0W68_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBI3074142.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5243..5911
                     /gene="hxpB"
                     /locus_tag="K0W68_002618"
     CDS             5243..5911
                     /gene="hxpB"
                     /locus_tag="K0W68_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBI3074143.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6074..6664
                     /locus_tag="K0W68_002619"
     CDS             6074..6664
                     /locus_tag="K0W68_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBI3074144.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6797..8188
                     /gene="tcyP"
                     /locus_tag="K0W68_002620"
     CDS             6797..8188
                     /gene="tcyP"
                     /locus_tag="K0W68_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HBI3074145.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(8192..9008)
                     /locus_tag="K0W68_002621"
                     /pseudo
     CDS             complement(8192..9008)
                     /locus_tag="K0W68_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9285..9548)
                     /gene="cedA"
                     /locus_tag="K0W68_002622"
     CDS             complement(9285..9548)
                     /gene="cedA"
                     /locus_tag="K0W68_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBI3074146.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9731..11992
                     /gene="katE"
                     /locus_tag="K0W68_002623"
     CDS             9731..11992
                     /gene="katE"
                     /locus_tag="K0W68_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBI3074147.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(12250..13008)
                     /gene="chbG"
                     /locus_tag="K0W68_002624"
     CDS             complement(12250..13008)
                     /gene="chbG"
                     /locus_tag="K0W68_002624"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBI3074148.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(13021..14373)
                     /gene="celF"
                     /locus_tag="K0W68_002625"
                     /gene_synonym="chbF"
     CDS             complement(13021..14373)
                     /gene="celF"
                     /locus_tag="K0W68_002625"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBI3074149.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14478..15320)
                     /gene="chbR"
                     /locus_tag="K0W68_002626"
     CDS             complement(14478..15320)
                     /gene="chbR"
                     /locus_tag="K0W68_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HBI3074150.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(15328..15678)
                     /gene="chbA"
                     /locus_tag="K0W68_002627"
     CDS             complement(15328..15678)
                     /gene="chbA"
                     /locus_tag="K0W68_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBI3074151.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15729..17087)
                     /gene="chbC"
                     /locus_tag="K0W68_002628"
     CDS             complement(15729..17087)
                     /gene="chbC"
                     /locus_tag="K0W68_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBI3074152.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(17172..17492)
                     /gene="chbB"
                     /locus_tag="K0W68_002629"
     CDS             complement(17172..17492)
                     /gene="chbB"
                     /locus_tag="K0W68_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBI3074153.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17791..18129)
                     /gene="osmE"
                     /locus_tag="K0W68_002630"
     CDS             complement(17791..18129)
                     /gene="osmE"
                     /locus_tag="K0W68_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBI3074154.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18331..19158
                     /gene="nadE"
                     /locus_tag="K0W68_002631"
     CDS             18331..19158
                     /gene="nadE"
                     /locus_tag="K0W68_002631"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBI3074155.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            19388..20275
                     /gene="cho"
                     /locus_tag="K0W68_002632"
     CDS             19388..20275
                     /gene="cho"
                     /locus_tag="K0W68_002632"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBI3074156.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20235..20810)
                     /gene="ves"
                     /locus_tag="K0W68_002633"
     CDS             complement(20235..20810)
                     /gene="ves"
                     /locus_tag="K0W68_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBI3074157.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         5611 a         5196 c         4823 g         5414 t
ORIGIN      
        1 acacgaatgc catttgcccc tatacatcca tcggcaatgt tgttaataat ggccacaggc
       61 cagactcaat agcttattac actattcaaa cagttgccca tactccctga aaaagaaatc
      121 attgaaataa taactgcgca gaatagcgtt ggtacacctg ctttatttct ggctatgatg
      181 aacggacaaa ctgacaacgt aaaaatattt atgcaagaaa ttcagtcact ggtatacaat
      241 catatcattc atgaagataa tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca
      301 cctggactct atatctccat gttgtatgga ttcgatgaaa taatcgatat ctttctgaat
      361 gcattaacca ctccaatagc acaagaactt ttaaacaaaa aaaatggtga tgagtatttt
      421 agccatgaaa atacatgatg gtgagccagg attatacgcc gcaatggaaa ataatcaccc
      481 tttgtgtgtc acacggttcc tctctaaaat taatggcatc gcctttaaat acaagttgag
      541 caaagctaac atcatggatt tattaaaagg agctacagca cagggaaccc ctgcattata
      601 catcgccatg agcaagggta atgaagacgt cgtgttatct tatatatcga cgctgggtgc
      661 ttttgcaaaa aaacattctt ttagtcaaca tcagttattt acactattgg ccgctaaaaa
      721 tcatgacaac atgtcagctg ttcatatagc cattcatcat aagcattata aaactgtaga
      781 aacatattat gctgctataa atgtcatcag ccaaagcctg agttttagtg ctgatgaaat
      841 aaagacgtat ttataaccag ctatttacga tatatatcga tacataattt tatttttttc
      901 ataaaacgca gaaacagtga ccatgtgcac aacgccaccc gacggctgta ataacaaaaa
      961 atcccttgat gcctatccct tttgttacac tccgttatca cgcacaagag atatgcagga
     1021 cactggtatg ccgactaaac gctttgataa aaaacactgg aagatggtgg tggtgctact
     1081 ggcaatctgt ggcgctatgt tgttgctacg ttgggcagca atgatttggg gctgagcagt
     1141 gacaaacgaa gacagccaga ccgcataaca cggtccggca gataatttta catgctgtag
     1201 cccagcgata aggttgtccg acgatcggta tgttccggtg cagactctgg aggttcagag
     1261 ttccatgtga cgttgtaagc caccttcagt ccaaaatgtt cattaatagc aacatttaat
     1321 gcgctttcgg agttcagcgt tgtatcttcc gcgccaaaga ccgaaacacc ctgcgtaaat
     1381 ttagcgttgt cagtcaactg ccatgcataa gccccggagg catagcccag cggctgggtt
     1441 tcactggcat tgtcggtata tttgtcgtaa cgcacacctg ggccgaattc aaagcggaaa
     1501 ctgtgtaccg ggccattcaa aaactggcga ccataacccg cggtcaacac atcgcgctct
     1561 cgatagccgt tataacggtc tgtcagccag cttgcctgac caaataaata gtcataatca
     1621 gttaaattaa aacggctacg cccgcccgcc gcatattttt ctgaagaacg ctcatcatta
     1681 gaagaagtat tactggcgtt cccccacagc gaccaggcag tggtttgtcc ataccaggtc
     1741 atggtggtgt cagccgtaag tgaggagctt ttcgtattgc ctgattgtgc aagatatcct
     1801 gcgttcagat taccttcgaa aggtttttta gcgctggcag gatcgtccat gacagtaaaa
     1861 acggaatcat cggcagctgc attcagtgac gcaaacatgc cccccgccaa cataacgatg
     1921 gcaggaactg tcttcaaaag cttcatttat caagagtccg tacaacaaaa aaagagacca
     1981 tcgcggtccc ggaaactttc ttaaggatca aagattagcg tccctggaaa ggtaacgaat
     2041 tataaaaagg cgcgaataac ttagcaatgt attcttattt cattttttga ataagcatgt
     2101 ggcgaaaaca gatttttatt tatatatatt tatctgcaaa attttaaata aagctccaat
     2161 aaatcatatt gttaatttct tcactttccg ctgattcggt gccagactga aatcagccta
     2221 taggaggaaa tgatggtacg tatctatacg ttgacacttg cgccctctct cgatagcgca
     2281 acaattaccc cgcaaattta tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa
     2341 cccgggggcg gcggcatcaa cgtcgcccgc gccattgccc atcttggagg cagtgccaca
     2401 gcgatcttcc cggcgggtgg cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa
     2461 aatgtccccg tcgctactgt agaagccaaa gactggaccc ggcagaattt acatgtacat
     2521 gtggaagcaa gcggtgagca gtatcgtttt gttatgccag gcgcggcatt aaatgaagat
     2581 gagtttcgcc agcttgaaga gcaagttctg gaaattgaat ccggggccat cctggtcata
     2641 agcggaagcc tgccgccagg tgtgaagctg gaaaaattaa cccaactgat ttccgcttcg
     2701 caaaaacaag ggatccgctg catcgtcgac agttctggcg aagcgttaag tgcagcactg
     2761 gcaattggta acatcgagtt ggttaagcct aaccaaaaag aactcagtgc gctggtgaat
     2821 cgcgaactca cccagccgga cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc
     2881 aaggccaaac gggttgtcgt ttcccttggt ccacaaggag cgctgggtgt tgatagtgaa
     2941 aactgcattc aggtggtgcc accaccggtg aaaagccaga gtaccgttgg cgctggtgac
     3001 agcatggtcg gcgcgatgac actgaaactg gcagaaaatg cctctcttga agagatggtc
     3061 cgttttggcg tagctgcggg gagtgcagcc acactcaatc agggaacacg tctgtgctcc
     3121 catgacgata cgcaaaaaat ttacgcttac ctttcccgct aacaaaaaca ttcccccagc
     3181 attgggggaa tcaccaccaa cctgtcggca acgcgtttct ccgactttgc tcaaaagtca
     3241 tgtgataaca aaggggtgaa ctatggccag tggcgatctt gtccgttatg tcataaccgt
     3301 catgttgcat gaggatacat tgactgaaat taacgagttg aataattacc tgactcgcga
     3361 cggttttttg ctcaccatga cggatgatga gggaaatatc catgagctgg ggactaacac
     3421 ttttggactt atcagtaccc aaagtgaaga agaaattaga gaactggttt cggggcttac
     3481 ccaaagtgca accggcaaag atcctgaaat caccatcacg acttgggagg aatggaatag
     3541 caacagaaaa taaatggttt ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt
     3601 tttacaccgc attcttgcgc taaccttatg atctggcaga caacatggga gagacatcat
     3661 gtggcaggca atcagtcgtc ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg
     3721 taatgaactg cctggcggag aagtccatgc cgcatggcat ttgcgctatg caggacatga
     3781 ctttttcgtc aaatgtgatg aaagggaact gcttcccggt tttaccgccg aagccgacca
     3841 actggagtta ctgtcgcgta gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc
     3901 tgaccgtgac tacagttttc tggtgatgga ttatctccca cctcgtccgc tggatgcgca
     3961 tagcgcattt attcttggtc agcaaattgc gcgtttacat caatggagtg accaaccaca
     4021 atttggcctc gattttgata actcgctctc cacaactcca cagcccaaca cctggcaacg
     4081 tcgctggtca acgttttttg ctgaacaacg gattggctgg cagttggaac tggcagcaga
     4141 gaaagggatc gctttcggca atatcgacgc catcgtcgag catattcagc agcgtctggc
     4201 ctcgcatcaa ctgcagcctt ctctgttgca cggcgattta tggtccggca actgtgcgct
     4261 gggtccggat ggcccgtaca ttttcgaccc tgcctgctac tggggtgacc gagagtgcga
     4321 cctggcgatg ttaccgctgc atactgaaca gccgccacaa atctatgacg gctatcagtc
     4381 agtatccccg ctacctgccg atttccttga acgtcaaccg gtttaccaac tctacacgct
     4441 gttaaatcgt gcaagattat ttggcggtca gcatttggtt attgctcagc agtcattgga
     4501 tagattatta gcagcatgat atgggttgag gataatggcc gctccgtgcg gccttttgat
     4561 taaataaacc caagcagaga aaagaacaca taacctgcag caatacaaat aaccggcagg
     4621 atatatagtg agaaaaactg caggaaaata gtatggtgcg gaacaacaat acgggactca
     4681 atttgttcac gcgttagccc ctcttcccct ttggcttttt ccagaatgag ttgatcttca
     4741 acaccttccc gcaagaagcg cgcctggcga ctcatccggg caccggaatc ctgcaacgcc
     4801 agtccgacaa aaatcaggat gaaaatcacc cagaacataa cgttcacgcc accattaaaa
     4861 ttaggggtcg gggagttata ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata
     4921 tcgatcatga catgagtaaa gtcgagcatg accgcattaa tgccttcctg gttttcctgc
     4981 cgggtattca taaatttcag caaagaaatc aatgtggaga tcaatgcagg tataaaaatc
     5041 acccaaccca aaatcctttt caaaacagca atgcgtccag cttgttgata cgtcatgagt
     5101 tctccttgat taagacgcgt cgtttcactt agtttacctg tagatatctg ttttcgccca
     5161 ttctttaaag gcgatatgat aggcgcttaa tcataagcac ggcttaatac cttacacata
     5221 atgctctaaa ggagaggttg taatgtcaac cccgcgtcag attcttgctg caatttttga
     5281 tatggatgga ttacttatcg actcagaacc tttacgggat cgagccgaac tggatgtgat
     5341 ggcaagcctg ggggtggata tctcccgtcg taacgagctg ccggacacct taggtttacg
     5401 catcgatatg gtggtcgatc tttggtacgc ccggcaaccg tggaatgggc caagccgtca
     5461 ggaagtagta gaacgggtta ttgcccgtgc catttcactg gttgaagaga cacgtccatt
     5521 attaccaggc gtgcgcgaag ctgttgcgtt atgcaaagaa caaggtttat tggtgggact
     5581 ggcctccgcg tcaccactac atatgctgga aaaagtgttg accatgtttg acttacgcga
     5641 cagtttcgat gccctcgcct cggctgaaaa actgccttac agcaagccgc atccgcaagt
     5701 atatctcgac tgcgcagcaa aactgggcgt tgacccactg acctgcgtgg cgctggaaga
     5761 ctcggtaaat ggcatgatcg cttctaaagc agcccgcatg cgttccatcg tcgttcctgc
     5821 gccagaagcg caaaatgatc cacgttttgt attagcaaac gtcaaactct catcgctgac
     5881 agaactcacc gcaaaagacc ttctcggtta atgaccaggg gcagtgatca cgctgcccct
     5941 ggttctttat ctgaatttgc attcaactga cggattaatc gtcaatttaa gagaaagagt
     6001 tacaccgtca ccacttccgt gcactgtata aaaatcctat actgtacgta tcgacagttt
     6061 agtgagtttt atcatgacgg cggaaggtca ccttctcttt tctattgctt gtgcggtatt
     6121 tgccaaaaat gccgagctga cgcccgtgct ggcacagggt gactggtggc atattgtccc
     6181 ttccgcaatc ctgacgtgtt tgttaccgga catcgatcac ccaaagtcgt ttcttgggca
     6241 gcgattaaaa tggatatcaa aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca
     6301 cagtctgctg gcggtatttg cgctgctggc aaccttttac cttaaggttc cggaaggctg
     6361 gttcattccg gctgatgcgc tacaaggaat ggtgctgggt tatttgagcc acatacttgc
     6421 cgatatgctg acacccgccg gtgttcccct gctctggcca tgccgctggc gtttccgctt
     6481 gcctatcctg gttccccaaa agggcaacca actggaacgt tttatctgca tggcattatt
     6541 tgtctggtcg gtatggatgc cccattcatt acccgagaac agcgctgttc gttggtcatc
     6601 gcaaatgatc aataccttgc agatccagtt tcatcggctt attaagcatc aggttgaata
     6661 ctaaaaaggc aaaaatcacc tttctggaat aagcaattcc atttgaatat aagagccagc
     6721 tcacagttct gttaatcttg cgccaacact atgactgcta cgcagtgata gaaataataa
     6781 gatcaggaga acggggatga actttccatt aattgcgaac atcgtggtgt tcgttgtact
     6841 gctgtttgcg ctggctcaga cccgccataa acagtggagt ctggcgaaaa aagtgctggt
     6901 gggtctggtg atgggtgtgg tttttggcct tgccctgcat accatttatg gttctgacag
     6961 ccaggtactt aaagattctg tacagtggtt taacatcgtt ggtaacggct atgttcaact
     7021 gctgcaaatg atcgttatgc cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct
     7081 gcataacgca tctcagttag gcaaaatcag ttttctgacc atcggtacgc ttttgtttac
     7141 cacgctgatt gcggcgctgg tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga
     7201 aggtctggtt cagggtggtg cagaaactgc acgtctgaac gccattgaaa gtaactatgt
     7261 tggtaaagtc tctgatctga gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc
     7321 gtttgccgat cttaccggag ccaatccgac gtcaattatc agcgtggtaa tttttgccgc
     7381 attcctcggc gtagctgcat tgaaactgct gaaggatgat gcgccgaaag gtgaacgcgt
     7441 cttagccgct atcgataccc tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca
     7501 gttgacccct tacggcgttc tggctctaat gaccaaagtg gttgcaggtt ctaacttgca
     7561 agacatcatc aaactgggaa gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt
     7621 tgcagtgcat ggcattctgc tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa
     7681 ggtatggcct gtgctgacgt ttgcctttac cagccgttcc agtgctgcgt ctatcccact
     7741 gaatgtggaa gcacaaacgc gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc
     7801 ctctttcggt gcaaccattg gtcagaacgg ctgcgccggt ttgtatccgg caatgctggc
     7861 ggtgatggtt gcgcctacgg ttggcattaa cccgctggac ccgatgtgga ttgcgacgct
     7921 ggtcggtatt gttaccgtta gttccgcagg cgttgccggt gtcggtggtg gtgcaacttt
     7981 cgccgcactg attgtgctgc ctgcgatggg cctgccagta accctggtgg cgctgttaat
     8041 ctccgttgaa ccgcttatcg acatgggccg tacggcgcta aacgttagtg gctcgatgac
     8101 agctggcacg ctgaccagcc agtggctgaa gcaaaccgat aaagccattc tggatagcga
     8161 agacgacgcc gaactggcac accgttaatt cttatgctgg caacggtccg ttttgtatag
     8221 gggccgttgc tttacttttt aaatgttcca tcgcggcgat gcatactgac tcatatttaa
     8281 acagatcgtc agcgaaaaaa atgtccaact tatcaataca ttcctggctc aatgaatgtg
     8341 cctgtagtga gaagcttaaa acctcatacc cgagacgttt tctaagtgaa ctatctggaa
     8401 tactcataat atcttcaagc acaccacttc ccattcccgg aagatttagt ggattaaaaa
     8461 gaagattgat attaagcatt gatattatcg aaggaaactt taggagactc tcattttttt
     8521 tgaatcgcag caaaaatgct cccggcagga tgtatataat ttttttcata gtatatagtc
     8581 cttaaataat gaataaccaa cctatttttt atattgatca aattcgtcat tttttcgggt
     8641 cggaattcac aggtaaaatc catcccaaca aaattctcat taatatctct tatccgaaac
     8701 tcactgccat aaagattatt aattatcact ctatatttaa cattgtcctg cagatacgta
     8761 tcaaaactaa gtgctggagc tcctggcttt gaggcatcat agttaatgtt agacatatag
     8821 gcaatcatac gattgaagtt cgactctgct atttcatcgc agtcacattg acccgttgat
     8881 ataaagaaag tgagtgccgc catggtagac tcaagccttt ctttaaaaga gtcgaaagcc
     8941 aggcttttaa tatttaaatc accataatta ctctgtatta agtttgtaga aaacatctcc
     9001 cgccccatat tgttaacaaa attattatct catttaaatc taagtcactt acaatataag
     9061 tttaagagcg acgccacagg atgaactatc aaaaatagct catcatgatt agcaaaactt
     9121 aaccatttta aaataaataa acaattaaag aaaaaagatc acttatttat agcaatagct
     9181 cgtcaaaggc agctttttgt tacaggtggt ttgaatgaat gtagcaacga aatgcagaat
     9241 ttcaggtcat gtaactcccg gcaaaaccgg gaagtatgta atccttactc agtcacttcc
     9301 ccttcctggc ggatctgatt tgcccaacgt tgggcagatt caggcacagt aaacgccggt
     9361 gagcgcagaa atgactctcc catcagtaca aacgcaacat atttgccacg cagcatccag
     9421 acatcacgaa acgaatccat ctttatcgca tgttctggcg gcgcgggttc cgtgcgtggg
     9481 acatagctaa taatctggcg gttttgctgg cggagcggtt tcttcattac tggcttcact
     9541 aaacgcatat taaaaattag aaaaactgta gtttagccga tttagcccct gtacgtcccg
     9601 ctttgcgtgt atttcataac accgtttcca gaatagtctc cgaagcggga tctggctggt
     9661 ggtctatagt tagagagttt tttgaccaaa acagcggccc tttcagtaat aaattaagga
     9721 gacgagttca atgtcgcaac ataacgaaaa gaacctacat cagcaccagt caccactaca
     9781 cgattccagc gaagcgaaac cggggatgga ctcactggca cctgaggacg gatctcatcg
     9841 tccagcggct gaaccaacac cgccaggtgc acaacctacc gcccctggga gcctgaaagc
     9901 ccctgataca cgtaacgaaa aacttaattc tctggaagac gtacgcaaag gcagtgaaaa
     9961 ttatgcgctg accactaatc agggcgtgcg catcgccgat gatcaaaact cactgcgtgc
    10021 cggtaaccgt ggtccaacgc tgctggaaga ttttattctg cgcgagaaaa tcacccactt
    10081 tgaccatgag cgcatcccgg aacgtattgt tcatgcacgc ggatcagcag ctcacggtta
    10141 tttccagcca tataaaagct taagcgatat caccaaagcg gatttcctct cagatccgaa
    10201 caaaatcacc ccagtatttg ttaggttctc taccgttcag ggtggcgctg gctctgcaga
    10261 taccgtgcgt gatatccgtg gttttgccac caagttctat actgaagagg gtatttttga
    10321 cctcgttggc aataacacgc caatcttctt tatccaggat gcgcataaat tccccgattt
    10381 tgttcatgcg gtaaaaccag aaccgcactg ggcaattcca caagggcaaa gtgcccacga
    10441 tactttctgg gattatgttt ctctgcaacc tgaaactctg cacaacgtga tgtgggcgat
    10501 gtcggatcgc ggcatccccc gcagttaccg caccatggaa ggcttcggta ttcacacctt
    10561 ccgcctgatt aatgccgaag ggaaggcaac gtttgtacgt ttccactgga aaccactggc
    10621 aggtaaagcc tcactcgttt gggatgaagc acaaaaactc accggacgtg acccggactt
    10681 ccaccgccgc gagttgtggg aagccattga agcaggcgat tttccggaat acgaactggg
    10741 cttccagttg attcctgaag aagatgaatt caagttcgac ttcgatcttc tcgatccaac
    10801 caaactgatc ccggaagaac tggtgcccgt tcagcgtgtc ggcaaaatgg tgctcaatcg
    10861 caatccggat aacttctttg ccgaaaacga acaagcagca ttccatccag gtcatattgt
    10921 tcctggtctg gacttcacca acgatccgct gttgcaggga cgtttgttct cctataccga
    10981 tacacaaatc agtcgtcttg gtgggccgaa tttccatgag attccgatta accgtccgac
    11041 ctgcccttac cacaacttcc agcgtgatgg catgcatcgt atggggatcg acactaaccc
    11101 ggcgaattac gaaccgaact cgatcaacga taactggccg cgcgaaacac cgccgggacc
    11161 gaaacgcggc ggttttgaat catatcagga acgcgtggaa ggaaataaag ttcgtgagcg
    11221 cagtccatcg tttggcgaat attattccca tccgcgtctg ttctggctaa gtcagacgcc
    11281 attcgagcag cgccatattg tcgatggttt cagttttgag ttaagcaaag tcgttcgtcc
    11341 gtatattcgt gagcgcgttg ttgaccagct ggcgcatatt gatctcactc tggcccaggc
    11401 ggtggcgaaa aatctcggta tcgaactgac tgacgaccag ctgaatatca ccccacctcc
    11461 ggacgtcaac ggtctgaaaa aggatccatc cttaagtttg tacgccattc ctgacggtga
    11521 tgtgaaaggt cgcgtggtag cgattttact taatgatgaa gtgagatcgg cagaccttct
    11581 ggccattctc aaggccctga aggccaaagg cgttcatgcc aaactgctct actcccgaat
    11641 gggtgaagtg actgcggatg acggtacggt gttgcctata gccgctacct ttgccggtgc
    11701 accttcgctg acggtcgatg cggtcattgt cccttgcggc aatatcgcgg atatcgctga
    11761 caacggcgat gccaactact acctgatgga agcctacaaa caccttaaac cgattgcgct
    11821 ggcgggagac gcgcgcaagt ttaaagcaac aatcaaggtc gctgaccagg gtgaagaagg
    11881 gattgtggaa gctgacagcg ctgacggtag ttttatggat gaactgctaa cgctgatggc
    11941 agcacaccgc gtgtggtcac gcattcctaa gattgacaaa attcctgcct gatgggaggc
    12001 ggcgcaattg cgccgcctca aatgatttac atagtgcgct ttgtttatgc cggatgcggc
    12061 gtgaacgcct tatccggcct acaaaactgt gcaaattcaa tatattgcag gaaacacgta
    12121 ggcctgataa gcgaagcgca tcaggcagtt ttgcgtttgt cagcagtctc aagcggcgca
    12181 gttacgccgc ctttgtagga attaatcgcc ggatgcaagg ttcacgccgc atctggcaaa
    12241 catcctcact tacacatccc gataactccc caaccgataa ccacgctgag cgatagcacc
    12301 tttcaacgac gctgatgtca acacatccag ctccgttaag cgtgggaaac agtaagcact
    12361 ctgacggata gtattatcga taaacgccgg atgacacatc acctccagcg aacgatcacc
    12421 ccgatggctt gcgtcatcca gcacttgcag gaacagtgcc tcgctaatct cttcaccata
    12481 aaacgcgcta ctgaatccct gagaactgcg cagattagcc ggtaaatcac cagagttaga
    12541 tagcggctga cggtcgatgc gcaacgcaat cccctcttca gccgcaaacc tggcaacaat
    12601 cgggaaaatc tgcgggaaca tatgcacatg atgatggcta tcaagatgcg taggtttgcg
    12661 tccaaatagc tcaatgaaac gcaaatactg actggcaagc tcctgagtaa tttcttccag
    12721 cggtaaagca tcttcttctg ccaactgcca gatccatttt cccagcacac catcgcgggt
    12781 taaccccggc atagctgtca gtggcttgcc catagtaagg acaaagtaca tccctatggc
    12841 cagactcggt tcatcacgac tcaactgcac cgcatggtca atagcctgcc cattcaccag
    12901 cgccgtcgtc gatgtgacaa tcccattgcg acaggcctcg ataatgccgt agttctgacc
    12961 tttgcttaag ccaaaatcat cggcattaac aatcagtaag cgttccataa tcagcctcgg
    13021 ttaatgtgct tttttaagct ctgcgatgca gtcggcaaaa tttggcagcc atttctcgtg
    13081 cgccagaatc atctcgcgtg ccagcagctc agcatcgcga tcggaatgca ccaacggact
    13141 aaggtttagc gccagtaaca catcgttaaa ttctccgcta agtgcggcgt tgctggcagc
    13201 aatctcgaag cctttaatgg tgtgaatcag ccccatcact ttatcatcga aatgcgtaat
    13261 gcgcggatgt ggcgtcgcgc catcgcgccc cagcttacag gtcatttcta ccgcccagtc
    13321 tgccggaata ttatcaatat gcccatgatg cgggatatta acgtaatgtt cagcttgctt
    13381 gtcgttgtag atagcgttga tcacttcgca cgctgcatca gagtaataag ccccaccgcg
    13441 ctgttccagt tctttcggct taactttcaa ctccgggttt ttatacagct caaaaagttg
    13501 tttctcgact ttctgtacta cctgcgctcg tgcgccgcct ttgtagtatt cgcccatttc
    13561 aatagccagc atctcttttt gcttgaagta ataaagcaaa taagaacacg gcaacagatt
    13621 cagagaacga attaagcctt cgctaaatgg cagatcgaaa atatttttaa cgccagatgc
    13681 ttttaactgc ccggatgcca caccatcaag caattcggca aagcgcgact tgccatttac
    13741 cagcacatcc ttaatgaaca ccatatggtt gaggccgaac aaatcgatag ataaatcatc
    13801 gctgtctttc agcatcagaa catcgtgaat aaacatcttc atgccgatcg gaatattaca
    13861 cacgccgata aagcgtttaa atccggtatg acgataaacg gcttcagtaa ccattcccgc
    13921 cgggttagtg aagttaatca cccatgcatt cggacaaagt tcttcgacat cttttacgat
    13981 gtcaaaaatc accggaatgg tacgcagacc tttaaacaga ccgcccgcgc cgttggtttc
    14041 ctggccaaga taaccatgac ttaatggaat acgttcatcc agttcgcgcg ccggtaattg
    14101 gcctacgcgc agttgggtag taacgaaatc agcatctttc aatgcttcgc ggcgatccag
    14161 cgttttataa agcttcatcg ggacgccagc gttatcaatc atccgttggc agagttcaaa
    14221 aatgatatcc agtttctctt taccatcttc gacatccacc agccataatt cgctgaccgg
    14281 caattcgtga taacgcttaa taaatccttc cagtaactcc ggggtatagc tgctcccgcc
    14341 accaatagtg acgactttta atttctggct cataatttct cccttcagta cagaatactg
    14401 atatctggca tatctgcccc cccggacata aataatccag caacaggaca gatatgtgaa
    14461 ttgtcaggta taacgactta ctgattaaat tcagtcaatt tcttacgata gctcttaggc
    14521 gtaaaggacg ttaatttctt aaacgtttta ataaacaaac tcgggctact ataaccggcc
    14581 tcaaacgcaa tatccgtcac ggaatagttg gtcatttcca gttgtttttt ggcaaaatta
    14641 atacggatct cattaataat ctgcattggt gttttgccat aatatcgttg agtcgctcgc
    14701 gtcaaatatt cctgtgattt ggctgacaac gtcaccatat tctccagcgc cgattcacta
    14761 aactgctctt tatcatgcat cttttctacc gtacttttca gccactgcgg tacatcatca
    14821 atcacctgtt cttcgcggta atgacgtaaa cggttaatga cataaaaagt aaccatctca
    14881 acaaactctt ccagccctgt ttcgcggaaa ttcaatgaag aaatcactgt ttccacatag
    14941 gtgagaaaag cgttattggt ccggtatacc tgcgaagcga caaagcaata aggcaacaac
    15001 ggcaggtaat gctgctcaaa aaagcgttta ctgatcccaa cgttcaatat acgcgtggca
    15061 ccaaactcat aaaaactttg atggtgcgaa cctaacggaa taaaaacaaa atcgccccgt
    15121 tccagtaaca cgcgcttacc gttaatctcc tggaaataac gcccggttaa taccagagta
    15181 aattcataat agtcgtgctg atgcagcccg ctgatactct cagttttgtt atagataaac
    15241 acatggaagt ttttgccatt aaacaactgc tgttctcggg cagtggcaat ttccggcgcg
    15301 ttaatcactg gctgcatcat cgactcctta tgccttcagt ttttcatgaa gctcaattaa
    15361 ttcagtaatc agttcacgcg caagcatgga cgtcattaaa tgatcctgag cgtggaccag
    15421 caccagactc actttcatct taccttcgcc cgcatcgcct tcaatcagtt tcgtctgtac
    15481 cagatgcgct tcattcaatg ccatccgtga ctgatccatc atggcttttg ctgcagcaaa
    15541 atcgccctgc ttcgcctgtt tcagtgccgc atacgccagg ctgcgcgctt gtccggagtt
    15601 gatgataagc cccatcacca cttcttccag ctcttcagct tccgtttgcg tatcgggaat
    15661 gttatcgaga tccatcatac atcgttcctc tctattctta ccggcacgat tacccgtacc
    15721 ggcatcgatt aaaatttcag ggcgttagcg atatcttctt cgcgctcttc tttatcaatc
    15781 gcgttttgtg ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatacca
    15841 aggttgaaga gtgcaaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc
    15901 agaccggttg gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag
    15961 tacgctgcga gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc
    16021 accgggttca tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg
    16081 gacggcagcg ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata
    16141 gccaggatca ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc
    16201 ggcttggccc agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat
    16261 tgctgatagg tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg
    16321 gtcagcgcca gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag
    16381 gcccagccca ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc
    16441 tgatggaagt tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga
    16501 ataataaagc cgggaattaa tgccgagaag gaacgcgata ctgaagcagg tacgctatcg
    16561 ggcaatttaa tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg
    16621 ccaataataa tcccggagat gatattcgct ccacctaacc agtttgcgcc aaccacatag
    16681 gcctcaccga cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc
    16741 gccagcgcat cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt
    16801 aaagacatta tccctaatgt tccgttatat acgttgccgc caataccttt caggccatta
    16861 agtgtttcaa tggttgaggc gtcgaggcga atacctaagg aataaaaaaa cgccccctcc
    16921 ccaaagctta gaaaaacgtt gttaattaat acaaacatgg cccccgcaag ggttaacggc
    16981 attaagcgaa taaagccatt tttgattgca ttaacgtgtg gctgctttcc tattttaact
    17041 gcaaaaggga ggagtacctt ttcaagcgat gcaataacat tactcataga aaaataccct
    17101 taaaaaccgc aatttaaata ttgcggtatt gatttatgaa ataactcttt gacgggaaaa
    17161 tttaaaataa attaatttgc tgcggctttt ttaatcgctg caaccgcagc cttaagcacg
    17221 cctaaaccat cgactttgcc ataaagcagc gagtcaatta cttcaaccgg tttgttgggt
    17281 aacaaacgct ggatttcggg caacatataa gcaatctgcg gccctaataa cacgacatcg
    17341 gcattctgac ctttttcacc agccagtgtt tccggaaatg cttcaataat gaccggaact
    17401 tcatattttt ctgcctgtgc gcgcattttt gataccagta aagaggtaga catgcccgca
    17461 gaacaaaaca gataaatgtg tttcttttcc ataaaactgc cctcatcgac gattatctgt
    17521 cagccagaca ctccgcaagc cttaacctgc ttccatgctc tgggtaactt gcgaaaccaa
    17581 catgactttt tttgtggctg aaaggagtat acggtataga cctgagcaat agtatttctt
    17641 tgaccttata aattgtttct ccttgacctg tggttatgac cctcttcaca tttcgggcaa
    17701 ataattcgcg aagataatta agaactcagg ccaaaaaaaa ccggcgcaat ggccggtttc
    17761 cgttgttact caagctttca gacgaattga ttacttcgca gcctgtggat cagtgtcgta
    17821 ttcagcacag gtctgataac cggagttgat gacatgtccg gtatcatcta acgcgacaaa
    17881 gtaggtttct gctttaccat cacgttgacc caggatgtag gtctggcaag taccacgagc
    17941 atggatcatg ctcacttcag acgaaggttt acccgcaatt tgtgcaacct gcgcccggct
    18001 catgcctttt ttgacgtctt tcaccacagg ctgtacaaac tggtctttag tacgatcata
    18061 agccgtacaa cccgccagca tggttaatac cgccgctgca ctcagaattc ctgccatatt
    18121 cttgttcata ttccgtcctc ttgtttatca gcgtgttaga taagcctgga atacattggg
    18181 cgctttttca agcccgcgaa cgaaacggct ccgctttcag aggattcctg tatgacgttt
    18241 taaccaccat tcagcccgct gtcgcttgtc gtttcagtgg caacgggtta gctttaagga
    18301 agttttgtct tttctgtctg gaggggttca atgacattgc aacaacaaat aataaaggcg
    18361 ctgggcgcaa aaccgcagat taatgctgaa gaggaaattc gtcgtagtat cgattttctg
    18421 aaaagctacc tgcaaactta tccgttcatt aaatcactgg tgctcgggat cagcggcggt
    18481 caggactcca cgcttgccgg aaagctgtgc cagatggcga ttaatgagct gcgcctggaa
    18541 accggcaacg aatcactgca atttattgcc gtacgcctgc cctatggtgt tcaggccgac
    18601 gaacaagatt gccaggatgc cattgccttt attcaaccgg atcgcgtatt aaccgttaat
    18661 atcaagggcg cggtattggc cagcgaacag gcattgcggg aagcaggcat tgaactgagc
    18721 gattttgtcc gtggcaatga aaaagcgcgt gagcggatga aagcacaata tagcattgcg
    18781 ggtatgacca gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat taccggattc
    18841 ttcactaaat atggtgacgg cggtacagac attaatccgc tgtatcgtct caacaaacgt
    18901 cagggtaaac agttactggc ggcattaggt tgcccggaac acctttataa gaaagcgcca
    18961 acggccgatc tggaagatga tcgcccttct ctgccagatg aagtggcact cggcgtgacc
    19021 tatgacaata tcgacgacta tctggaaggg aaaaacgtac ctgaacaggt cgccagaaca
    19081 atagagaact ggtatctgaa aaccgaacat aaacgccgtc cgccaattac cgttttcgat
    19141 gatttctgga aaaagtaata atttgcacat aatggtttgt gcgaaaaaga gtaatttgtt
    19201 cacgccggat gcggcgtgaa cgccttattc gacctacaaa actatgcaaa ttcaatatat
    19261 tgcaggagtc gaataggcct gacaggcgta gcacgtcaga cggtgtaacc tttgtcatcg
    19321 acccgcctct tttttaatcg ctccccgcct gttacactgg atagataacc agcattcgga
    19381 gtcaacagtg gtacggcgtt taacttctcc gcggctcgaa tttgaagctg cggcaattta
    19441 tgaatatccc gaacatttac gttcattcct taatgactta cccacccgac caggggtgta
    19501 tctgtttcat ggcgaaagtg acaccatgcc gctctatatc ggcaaaagcg ttaatatccg
    19561 cagtcgggtg ctttctcatt tacgcacccc ggatgaagcc gccatgctac ggcaatcccg
    19621 gcgaattagc tggatctgta ccgcaggcga aatcggcgct ctgctccttg aagcgcgatt
    19681 aatcaaagaa caacagccgc tgtttaataa acggttgcgc cgcaatcgcc agctctgtgc
    19741 cctgcaatta aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg atttttcacg
    19801 agcccccaac ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag cattgcagac
    19861 catcgctgat gaacaaaaac tttgttatgg cctgctggga ctggaaccgt taagtcgcgg
    19921 tcgtgcatgt tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg gtaaagagag
    19981 ccatgaggaa catgcgctac gcttgcgcca gtctctggag cgtttgcggg tggtgtgttg
    20041 gccttggcaa ggtgcggtgg cactgaaaga acagcacccg gaaatgactc aatatcatat
    20101 tattcaaaac tggctgtggc tgggggcggt taattcgctg gaagaagcga caacgttaat
    20161 tcggacaccc gccgggtttg atcacgacgg ttataaaatt ctttgtaagc cgctgctttc
    20221 cggtaactat gaaattactg aacttgatcc ggcgaatgac cagcgagcca gttgatttca
    20281 ctgaacaaca gtttaccttg cggttgtaaa agccgcaggg tgtgcctgcc atcgaaccag
    20341 cacgcgccct ggtcggtagt gagcaattta tcgcccagtt gccacgcccc gttaatcaca
    20401 aacaccaccc cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat tctgactttc
    20461 gccttacaga catccaggcg tgtcatgatg ttgaaatcca tcgacatctg cccttccgtc
    20521 agtttcgctt ttaccacctg gtccgctgca aaggcaaaag gctgtaacgg ttttaaggta
    20581 tggttaaagc ggtctgcgct ttcaaggagc atctcgccgc cttccagcaa cgtcactatc
    20641 ctttccatgc cgggaaacaa agaaaactcc ccattcgccg cgatggatgc aatgctggca
    20701 cgccagtaaa aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt ttcaccggca
    20761 gcatttcgcc acagattcac cgacatttta cgcatatcaa agtattccat actcgctccc
    20821 ggcccgttct tttcattgtt atcgttcagg taaccgtgga tggttatcgg caactcgggt
    20881 gatttacgtt agtggtgatc aggctttttt tacaactgtt tgagaagaga aaagaaaacc
    20941 gccgatcctg tccaccgcat tactgcaagg tagtggacaa gaccggcggt cttaagtttt
    21001 ttggctgaaa gattattcag cagttgcagg cattttacct tttg
//
LOCUS       DAETQN010000071        21003 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.071, whole genome shotgun sequence.
ACCESSION   DAETQN010000071 DAETQN010000000
VERSION     DAETQN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.071"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..170)
                     /locus_tag="K0W68_002634"
     CDS             complement(<1..170)
                     /locus_tag="K0W68_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074158.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="K0W68_002635"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="K0W68_002635"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBI3074159.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="K0W68_002636"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="K0W68_002636"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBI3074160.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="K0W68_002637"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="K0W68_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBI3074161.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="K0W68_002638"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="K0W68_002638"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBI3074162.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="K0W68_002639"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="K0W68_002639"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBI3074163.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="K0W68_002640"
     CDS             4288..5151
                     /locus_tag="K0W68_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBI3074164.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="K0W68_002641"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="K0W68_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBI3074165.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="K0W68_002642"
     CDS             6489..7106
                     /locus_tag="K0W68_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBI3074166.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="K0W68_002643"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="K0W68_002643"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBI3074167.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="K0W68_002644"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="K0W68_002644"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBI3074168.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="K0W68_002645"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="K0W68_002645"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBI3074169.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="K0W68_002646"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="K0W68_002646"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBI3074170.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="K0W68_002647"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="K0W68_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBI3074171.1"
                     /translation="MNPTRYARICEMLARWQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="K0W68_002648"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="K0W68_002648"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBI3074172.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="K0W68_002649"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="K0W68_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBI3074173.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16560..17951
                     /gene="xanP"
                     /locus_tag="K0W68_002650"
     CDS             16560..17951
                     /gene="xanP"
                     /locus_tag="K0W68_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBI3074174.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTTLKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18072..19781
                     /locus_tag="K0W68_002651"
     CDS             18072..19781
                     /locus_tag="K0W68_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBI3074175.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="K0W68_002652"
     CDS             complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="K0W68_002652"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBI3074176.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5083 a         5712 c         5385 g         4823 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 tctacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgatgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggtt
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttacgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaattt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcctg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggtggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggta tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg
    16501 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    16561 tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg
    16621 aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc
    16681 agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc
    16741 tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg
    16801 tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg ttgtctattc
    16861 agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccacg ctgaaaaccg
    16921 gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt
    16981 gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg
    17041 tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca
    17101 ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc
    17161 tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac
    17221 gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg tttatgggca
    17281 tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct
    17341 attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca
    17401 cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccagtgt
    17461 ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt
    17521 ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac aacggagtga
    17581 tccagttgac cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg
    17641 tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa ccggttctgg
    17701 gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc
    17761 gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg
    17821 gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct
    17881 cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag
    17941 aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttaac
    18001 cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg
    18061 gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag
    18121 tggcgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa catatcagcg
    18181 catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggtt caccgttttt
    18241 ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc cagcccgcga
    18301 ccctggtggc caaaagtgtc gacattgcgc taagtagtcg gcaactgacc gaaccacgcc
    18361 atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc
    18421 taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg
    18481 aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg
    18541 gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg
    18601 atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga
    18661 ctaacctggg tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag cgtaacgccg
    18721 acggcagctg gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat
    18781 cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg
    18841 aagtgatcga tgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct
    18901 tgcgcaacat gaccttcagt aaagatgact ggcagacaca agaaggcaaa ctgtcgatga
    18961 acgctagcga gttcatttat ggttcgctgc atttatttga cccgattata aacgcggaat
    19021 tttctccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca
    19081 gaacgtcagg gaactggctg cgtgacggga aaacgttgat ccttgatgat gcggcaattg
    19141 ccggactgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa acgacacccg
    19201 gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata
    19261 tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc
    19321 tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat gccgccgccg
    19381 cgacgtttaa tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca
    19441 cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca
    19501 gtgtttcaca aacgccacaa cgtcagacac atatcagcct gaatggacgc ggtgtgccgg
    19561 tgaatatttt gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc
    19621 agcttaccgc cagtggcgat attcaggcca atgtcccgtt gaaacctacg gttagcgggc
    19681 aattgcatgc cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat gcgggcgtcg
    19741 tttccagcgg tgaagttaca tcggcagagc tggtgcagta aacatcaatc tcgccgggta
    19801 cgactcagac gtacccggca ttcttcaata gattacaacg taattgtcag cgcattccct
    19861 tgaggcgtaa ccaccagccc ctgctcactt tcagcctgcg aaccgccttg cagaccattt
    19921 actttcacaa tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc accagtcaca
    19981 gtaatcgtgt taccagtacg tgttgctttt agagtaaaga tcaccgaacc gtcagcagcg
    20041 ggcacttcac atacagcttc atgcccgtct tgcagattaa agagatggaa tgccgtgcct
    20101 tcgtgccacg cgtaatcggg acgttgatcg ttgttgccca gcgccaatag ggtgttatca
    20161 cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg
    20221 agctcattgt tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa ctgcacctcg
    20281 cccgcctcag tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta ttgacggtca
    20341 aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta
    20401 ccccgcgctt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt
    20461 tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg cacacgataa
    20521 gatttgctgc catgtaaacg actatggctg gagagcaaac caaacgcgca ccagcgtttg
    20581 taaacgtgtg ccggcgcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa
    20641 cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg
    20701 taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca
    20761 aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg
    20821 tagatgtacg cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca
    20881 tcagttggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga
    20941 cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc
    21001 aga
//
LOCUS       DAETQN010000072        20711 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.072, whole genome shotgun sequence.
ACCESSION   DAETQN010000072 DAETQN010000000
VERSION     DAETQN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.072"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="K0W68_002653"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="K0W68_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBI3074177.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="K0W68_002654"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="K0W68_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBI3074178.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="K0W68_002655"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="K0W68_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBI3074179.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="K0W68_002656"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="K0W68_002656"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBI3074180.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="K0W68_002657"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="K0W68_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBI3074181.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="K0W68_002658"
     CDS             complement(8214..9107)
                     /locus_tag="K0W68_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBI3074182.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="K0W68_002659"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="K0W68_002659"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBI3074183.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="K0W68_002660"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="K0W68_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBI3074184.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="K0W68_002661"
     CDS             complement(10238..11470)
                     /locus_tag="K0W68_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBI3074185.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="K0W68_002662"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="K0W68_002662"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBI3074186.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="K0W68_002663"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="K0W68_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBI3074187.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16539)
                     /locus_tag="K0W68_002664"
     CDS             complement(14275..16539)
                     /locus_tag="K0W68_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HBI3074188.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="K0W68_002665"
     CDS             complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="K0W68_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBI3074189.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="K0W68_002666"
     CDS             complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="K0W68_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBI3074190.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="K0W68_002667"
     CDS             complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="K0W68_002667"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBI3074191.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="K0W68_002668"
     CDS             complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="K0W68_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBI3074192.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4971 a         5732 c         5044 g         4964 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttcctg
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaagcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacacc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgct atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    15181 ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc agaaaagtaa
    15241 agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg gcccgcttaa
    15301 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15361 taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcacggg
    15421 cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15481 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15541 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15601 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15661 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15721 ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc agtggatcca
    15781 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acaccgcaaa
    15841 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15901 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15961 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    16021 attcggttca ataagacttg cctgcagaaa gcggccggtg agcggctgat gctgggcaat
    16081 ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg cacgaacttt
    16141 gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt cgccatacag
    16201 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16261 agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16321 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16381 cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc aggcgaagag
    16441 agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca acggaaaaat
    16501 tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc tgtttatgga
    16561 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16621 cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16681 ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16741 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16801 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16861 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16921 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16981 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    17041 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    17101 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    17161 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    17221 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17281 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17341 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt agcgtcttca
    17401 acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17461 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17521 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17581 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17641 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17701 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17761 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17821 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17881 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17941 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    18001 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    18061 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    18121 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    18181 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18241 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18301 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18361 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18421 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18481 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18541 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18601 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18661 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18721 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18781 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18841 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18901 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18961 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    19021 tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggat gccggaacca
    19081 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    19141 gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    19201 cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc accgttccca
    19261 tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19321 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc acttcctgag
    19381 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggttg
    19441 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct gacgcaacat
    19501 caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc gaatccagca
    19561 gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19621 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19681 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19741 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19801 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat g
//
LOCUS       DAETQN010000073        20399 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.073, whole genome shotgun sequence.
ACCESSION   DAETQN010000073 DAETQN010000000
VERSION     DAETQN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.073"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(130..1779)
                     /gene="actP"
                     /locus_tag="K0W68_002669"
     CDS             complement(130..1779)
                     /gene="actP"
                     /locus_tag="K0W68_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBI3074193.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1776..2089)
                     /locus_tag="K0W68_002670"
                     /pseudo
     CDS             complement(1776..2089)
                     /locus_tag="K0W68_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2289..4247)
                     /gene="acs"
                     /locus_tag="K0W68_002671"
     CDS             complement(2289..4247)
                     /gene="acs"
                     /locus_tag="K0W68_002671"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBI3074194.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4641..6077
                     /gene="nrfA"
                     /locus_tag="K0W68_002672"
     CDS             4641..6077
                     /gene="nrfA"
                     /locus_tag="K0W68_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBI3074195.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6122..6688
                     /gene="nrfB"
                     /locus_tag="K0W68_002673"
     CDS             6122..6688
                     /gene="nrfB"
                     /locus_tag="K0W68_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBI3074196.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6685..7356
                     /gene="nrfC"
                     /locus_tag="K0W68_002674"
     CDS             6685..7356
                     /gene="nrfC"
                     /locus_tag="K0W68_002674"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBI3074197.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7353..8309
                     /gene="nrfD"
                     /locus_tag="K0W68_002675"
     CDS             7353..8309
                     /gene="nrfD"
                     /locus_tag="K0W68_002675"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBI3074198.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8329..10047
                     /locus_tag="K0W68_002676"
     CDS             8329..10047
                     /locus_tag="K0W68_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBI3074199.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSHSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10040..10423
                     /gene="nrfF"
                     /locus_tag="K0W68_002677"
     CDS             10040..10423
                     /gene="nrfF"
                     /locus_tag="K0W68_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBI3074200.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10420..11016
                     /gene="nrfG"
                     /locus_tag="K0W68_002678"
     CDS             10420..11016
                     /gene="nrfG"
                     /locus_tag="K0W68_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBI3074201.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11358..12671
                     /gene="gltP"
                     /locus_tag="K0W68_002679"
     CDS             11358..12671
                     /gene="gltP"
                     /locus_tag="K0W68_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBI3074202.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12750..13439)
                     /locus_tag="K0W68_002680"
     CDS             complement(12750..13439)
                     /locus_tag="K0W68_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBI3074203.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13533..15680)
                     /gene="fdhF"
                     /locus_tag="K0W68_002681"
     CDS             complement(13533..15680)
                     /gene="fdhF"
                     /locus_tag="K0W68_002681"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15261..15263),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBI3074204.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15878..17344)
                     /gene="mdtP"
                     /locus_tag="K0W68_002682"
     CDS             complement(15878..17344)
                     /gene="mdtP"
                     /locus_tag="K0W68_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBI3074205.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17341..19392)
                     /gene="mdtO"
                     /locus_tag="K0W68_002683"
     CDS             complement(17341..19392)
                     /gene="mdtO"
                     /locus_tag="K0W68_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBI3074206.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19392..>20399)
                     /gene="mdtN"
                     /locus_tag="K0W68_002684"
     CDS             complement(19392..>20399)
                     /gene="mdtN"
                     /locus_tag="K0W68_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBI3074207.1"
                     /translation="PRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVV
                     PEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSVD
                     AQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADL
                     NAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSV
                     GQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSI
                     GYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEP
                     Q"
BASE COUNT         4555 a         5435 c         5601 g         4808 t
ORIGIN      
        1 atcattttct acgactgtca aaaatcgctc attttttaat gagtttattt gtttaatatt
       61 ttgggaaaag gtgatgcatg ctggagagga agagtattcc ccggtcaggc gaccggggaa
      121 ggggagagat taatgtgcgc ggccttgctc aacgccaaag ccggtctggg agcggataaa
      181 ctgcgcgcgg aacagttcac gctcgcgcgc cccttccgct gagttatcgg ttgccgagaa
      241 gaaccagatg ccgaggaatg ccacggtgat agagaacagc gccgggtatt catacgggaa
      301 gatggctttt tcgtgaccaa ggatctgtac ccaaatcgtc gggccgagga tcatcagtac
      361 tactgcggta atcagcccca gccagccacc catcatcgcg ccacgcgtgg tcagtttcga
      421 ccagtacata gaaagcagaa tgatcgggaa gttacagctc gccgcgatgg caaacgccag
      481 ccccaccata aaggcgatgt tctgattctc aaacagcacg ccgaggataa tcgcaatcac
      541 gccgaggatc agtacggtga tttttgatac ccgcagctct tcacgttcgg tcgcgccttt
      601 tttgaagacg ttagcgtaca agtcatgcga aaccgcggat gcgcccgcca gcgtcagacc
      661 cgcaaccacc gcgaggatag tggcgaaagc aaccgctgaa ataaaaccga ggaacaggtt
      721 gccgcccact gcattcgcca ggtgaacggc cgccatgttg ttaccaccaa tcagatggcc
      781 cgccgcgtct ttatattccg gattcgcacc aaccagcatg atcgcgccga agccgataat
      841 aaaggtcaga atatagaagt agcccataaa cccggtggcg tagaacacgc tcttacgtgc
      901 ttcgcgggca tcgctgactg taaagaagcg catcagaatg tgcggcaagc ccgccgtacc
      961 aaacatcagt cccagaccca gagagagtgc ggagatcgga tctttcacca gcccgcccgg
     1021 cttcatgatg tcgacacctt tcgggtgtac cgccatcgct tcactgaaca gattgttgaa
     1081 gctaaagccg acgtgtttca tcaccataaa ggccataaag ctggcaccga acagcaacag
     1141 cacggctttg ataatctgta cccaagtggt cgccagcatg ccgccgaaca ggacgtacat
     1201 catcatcagc acgccgacca gcaccaccgc aatgtgatag ttaaggccaa acagcagctc
     1261 gatcagttta cctgcgccca ccatctgggc gataaggtaa agcgccacca ccaccagaga
     1321 accacaggcc gaaagaatac gaatcggccc ttgtttcaga cggtaagagg ccacatcggc
     1381 aaaggtgtag cgccccaggt tacgcagacg ttcggcaatc aggaacaaaa tgatcggcca
     1441 gcccaccagg aagcccagcg agtaaattaa gccgtcatag ccggaggtaa acaccagcgc
     1501 ggagatcccc aagaatgagg cggcggacat atagtccccg gcaatcgcca gcccgttctg
     1561 gaagccagtg atattgccgc ctgcggtgta gtagtcgcta cgagaacgta cgagttttga
     1621 tgcccagtag gtaatgccga gcgtaaacac gacgaaaatc aggaacataa taatcgcctg
     1681 ccagttcgtt ggctggcgct ctacggcccc gctaatagca tccgcggcgt tagctgcgaa
     1741 agggagtgtg gcggcaagcg ccgtcagaac tcttttcatg atgcttgtac ctcatgcagg
     1801 acttcattat taagacggtc gaattcgccg ttcgcccgcc agatgtagat accggtgaga
     1861 acaaaggaga tcacaatcac tccaacacca attggaatac cgcgtgtgac gctggtgttc
     1921 ggattcagcg gcgtgccagc cagccgggcg cgaaggcgat cagtaaaata aagccgatat
     1981 aaactgccag cataataatc gacaggatgg tggcaaaccg ttgccgtttt tcgactaact
     2041 ccctgaaatg cgcattgtct tctatccgct gataaatagt gccattcatc acagattctc
     2101 cagaggtaat gtagggattg ttttaattcc cgctccctta tgggagaagg ttaacgctcg
     2161 ggtaaccctt gccgaatgta ggccggataa ggcgtttacg ccgcatccgg caatcaatgc
     2221 ctgatgcgac gctgtcgcgt cttatcaggc ctacaaaccg ttaccgactc gcatccggca
     2281 attgtgggtt acgatggcat cgcgatagcc tgcttctctt caagcagctt ctcgactacg
     2341 ccaggatcgg caagcgtcga ggtatcgccc aggttgctgg tatcgcccgc cgcaattttg
     2401 cgcagaatac ggcgcataat tttgccggag cgggttttag gcagggagtc ggtccagtgc
     2461 agcacgtctg gcgtcgccag cgggccaatc tctttacgca cccagttgcg gacttctgcg
     2521 tacagttctg gtgacggttc ctccccgtga ttaagcgtga cgtaggcgta gatcgcctga
     2581 cctttaatat tgtgcggaat acctactacg gcggcttcgg caatcttcgg atgcgccacc
     2641 agcgccgact caatctctgc cgtccccaga cggtgaccgg agacgttcag cacgtcgtcc
     2701 acacgcccgg tgatccagta atagccatct tcatcgcgac gcgcgccgtc gccgctgaaa
     2761 tacatatttt tgaaggtgga gaagtaggtc tgttcaaaac gttcgtgatc gccaaacagc
     2821 gtacgcgcct gacccggcca ggagtcggtg atcaccaggc tgccttcggt agcgccttcc
     2881 agcgggttac cttcgttatc gaccagcgcc ggttgcacgc cgaagaacgg acgtgttgcc
     2941 gaaccggctt tcagctcggt agcgccaggc agcggcgtga tcatgaaacc gccagtttcg
     3001 gtctgccacc aggtatcgac caccggacat ttctcgttgc cgattttttt ccagtaccac
     3061 tcccacgctt ccgggttaat tggctcgccc acggaaccga gaatgcgcag cgacgaacgg
     3121 tcggtaccct cgatcgcttt atcgccttcc gccatcagcg cgcggatcgc cgtgggtgcg
     3181 gtatagagaa tattgacctg atgcttgtcc accacctgcg ccatacgggc aggcgtcggc
     3241 cagttgggta cgccttcaaa catcagcgtg gtcgcaccgc aggccagcgg gccgtacagc
     3301 aagtaactgt gtccggtcac ccagcccaca tcggcggtgc accagtagat atcacccgga
     3361 tgataatcaa agacatattt aaaggtcagc gccgcgtaca ccagataacc gccggtagta
     3421 tgcagcacac cttttggctt accggtagaa ccggaggtgt agagaataaa cagcggatct
     3481 tcggcgttca tcttttccgc ctggtgctga tcgctcgctt gctcaaccag gtcgtgccac
     3541 cacaggtcgc gcccttcctg ccagtcaatt ttcccgccag tacgcttcag taccaccaca
     3601 tgctctacgc tggtgacgtt cgggtttttc agcgcgtcat caacgttttt cttcagcgga
     3661 atactgcgcc cggcacgcac accttcgtcg gaagtgatca ccagtcgtga gttggaatca
     3721 ataatgcgcc cggcaacggc ttccggcgag aagccgccga aaatcaccga atgcaccgcg
     3781 ccaatgcggg cgcaggccag catcgcaacc gcggcttccg gcaccatcgg catataaatc
     3841 gccaccacat cacctttttt aatgcccagc tcgagcaggg tattggcgaa gcggcagacg
     3901 tcgcggtgca gctctttata gctgatatgt ttgctctggc tggcgtcgtc gccttcccag
     3961 atgatggcgg tacgatcgcc gttttcttgc agatggcggt caaggcagtt tgccgccaga
     4021 ttcagcgtgc cgtcctcgta ccatttaatg gacacattac cgggggcaaa ggaggtgttt
     4081 ttcaccttct ggtaaggttt gatccagtca agaatttttc cctgttcgcc ccagaaggta
     4141 tcaggtacgt taatagattg ttgatacatc gcctcgtact gctgagggtt tatcaggcaa
     4201 cggtctgcga tgttggcagg aatggtgtgt ttgtgaattt ggctcatgct tttgttctcc
     4261 ttgtagggtg ttaataatat gtggcataag cgttaaatgt aggggtattg gcagttttgt
     4321 ttagtatttg ggcgacagat cacgcaaaag tagaattgtg caaataaacg gcagggtaat
     4381 ttttgaaggt cagcaacaaa agttgattaa ttctttgagg aacatgcagt tatgcattgc
     4441 tgttgaaaaa gaggaagata ctaactaact ctaaagtggt attttacatg cacttacaat
     4501 tgattaaaga caacattcac agtgtggtta tttgttacac ataggggcga gcaatgtcat
     4561 gacagtgtag gtgcggttac tgtcgtgaaa aacaataaaa accgccattg taacaatggc
     4621 gcaattcgga tgaagcccct atgacaagga taaaaataaa cgcacgccgt atcttcagct
     4681 tattgattcc ttttttcttt ttcacttctg ttcacgctga acaaacggca gctcccgcaa
     4741 aacctgtaac tgtggaagcg aagaatgaaa cctttgcccc gcagcatccc gatcaatatc
     4801 tctcctggaa agccacctcg gaacagtcag agcgtgttga cgccctggcg gaagatccac
     4861 ggctggtgat cctgtgggcg gggtatccct tctcgcgcga ttacaacaag ccgcgtggac
     4921 atgcctttgc tgtgaccgat gtgcgtgaaa ccctgcgtac cggtgcgccg aaaaacgctg
     4981 aagatggtcc gctaccgatg gcgtgctgga gttgtaaaag cccggatgtg gcgcgtctga
     5041 tccagaaaga cggcgaagat ggctacttcc acggtaagtg ggcgcgcggc ggcccggaaa
     5101 tcgtcaacaa cttaggttgt gccgactgcc ataacaccgc ctcaccagag ttcgccaaag
     5161 gcaaaccgga gttaaccctt tcccgtccgt atgcggctcg cgcgatggaa gccattggta
     5221 aaccttttga gaaagccgga cgtttcgacc agcaatcgat ggtttgcggt cagtgccatg
     5281 tggagtatta cttcgacggc aaaaacaaag cggttaaatt cccgtgggat gacggcatga
     5341 aagtcgaaaa tatggagcag tattacgaca aaattgcctt ctctgactgg actaactccc
     5401 tgtcgaaaac gccaatgctg aaagcgcagc acccggaata tgaaacctgg acagcgggca
     5461 ttcacggtaa aaacaacgtg acctgtatcg actgccatat gccaaaagtg cagaacgccg
     5521 aaggcaaact ctataccgac cataaaattg gtaatccgtt tgataacttc gcccagactt
     5581 gtgcgaactg ccatacccag gacaaagctg ccttgcaaaa agtggtcgcg gaacgtaagc
     5641 agtcgattaa cgacctgaaa atcaaggttg aagatcaact ggttcacgct cacttcgaag
     5701 cgaaagcagc gctggatgca ggcgcgacgg aagctgaaat gaagccaatt caggacgata
     5761 tccgtcatgc ccagtggcgc tgggatctgg cgatcgcttc ccacggcatt catatgcacg
     5821 caccggaaga aggtttacgg atgctcggta cggcgatgga taaagcggcg gatgcacgca
     5881 ccaaactggc gcgcctgctg gcgaccaaag gcatcaccca tgaaatccag atcccggata
     5941 tctcaaccaa agagaaagcc cagcaggcca ttggcctgaa catggaacaa atcaaggccg
     6001 agaagcagga cttcatcaaa acggtgatcc cgcagtggga agaacaggca cgtaaaaacg
     6061 gtctgttaag ccaataaccc cgttccgcct cgcaaggggc ggaaaacaca atggagtgaa
     6121 tatgagcgta ttacgttcgt tattaactgc cggggtgctg gcgtcgggcc tgttgtggag
     6181 cctgaacgga attaccgcta cccctgcggc gcaggcatct gatgatcgct acgaagttac
     6241 ccagcagcgt aacccggatg ccgcctgtct ggactgtcat aaaccagata ccgaaggtat
     6301 gcatggcaaa catgcttccg ttatcaaccc gaataacaaa ctgccggtca cctgcaccaa
     6361 ctgtcacggc cagccatcac cgcaacaccg cgaaggggtg aaagatgtga tgcgctttaa
     6421 cgagccgatg tacaaggttg gagagcagaa cagcgtctgt atgtcctgtc atctgccgga
     6481 acagttgcaa aaagcgttct ggccgcacga tgtccacgta accaaagtgg cgtgcgccag
     6541 ctgccattcc ctgcatccac agcaagatac gatgcagacg ttaagtgaca aaggacggat
     6601 taagatttgc gtcgattgcc acagcgatca gcgcaccaat ccgaacttta acccggcgtc
     6661 tgttccgttg cttaaggagc agccatgacc tggtctcgtc gccagtttct caccggcgtc
     6721 ggcgtgctgg cagccgtcag cggcaccgca gggcgcgtgg tggcgaaaac gttgaatatc
     6781 aatggggtgc gttacggcat ggtgcatgac gagtcgttat gcatcggctg taccgcctgt
     6841 atggatgctt gtcgggaagt gaacaaggtg ccggaaggcg tctcgcgcct gacgattatt
     6901 cgtagcgagc cgcagggcga atttcctgat gtgaaatatc gcttcttccg taagtcttgc
     6961 cagcactgcg atcatgcgcc gtgcgttgac gtctgcccga ccggtgcgtc ttttcgtgat
     7021 gccgctagcg ggattgtcga tgttaatcct gatctctgcg tcggttgtca gtactgcatc
     7081 gccgcctgtc cgtaccgcgt gcgctttatc catccggtca cgaagacggc ggacaaatgc
     7141 gatttctgcc gtaaaaccaa tttgcaggcc ggtaagctgc ccgcgtgtgt tgaggcttgc
     7201 ccgacgaagg cgctgacgtt tggcaatctg gacgatccta acagtgagat ttcgcaactg
     7261 ctgcgccaga agcctactta ccgctacaag ctggcgctgg gaaccaaacc gaagctgtac
     7321 cgcgtaccgt ttaaatacgg ggaggtgagc caatgacgca gacttccgca tttcattttg
     7381 aatcgctggt gtgggactgg ccgattgcca tctacctgtt tttgattggt atttctgccg
     7441 gtctggtgac gctggccgtg ctgttacgtc gcttctaccc gcaggcgggc ggtgcagaca
     7501 gtacgttgct gcgcaccacg ctgattgtcg ggccgggcgc ggtgatcctc ggtctgttga
     7561 tcctcgtctt ccacctgaca agaccgtgga ccttctggaa gctgatgttc cactacagtt
     7621 ttacctcggt gatgtcgatg ggggtgatgc tgtttcagct ctacatggtg gtgctggtgc
     7681 tgtggctggc gaaaatcttt gaacatgatt tgcttgccct gcaacaacgc tggttgccga
     7741 agctggggat cgtgcaaaag gttctgagcc tgctgacgcc cgttcatcgc ggactggaaa
     7801 cgctgatgct ggtgttggcg gtgctgctgg gggcttatac cggctttctg ctgtcggcgc
     7861 tgaaatcgta tccgttcctc aataacccga tcctgccggt gctgttcctc ttctccggca
     7921 tctcgtccgg tgcggcggtg gcgctgatcg ccatggcgat acgccaacgc agtaacccgc
     7981 attccacgga agcgcacttt gttcaccgga tggaaatccc cgtggtatgg ggtgaaatct
     8041 tcctgctggt ggcgtttttt gtcggtctgg cgctgggcga tgacggtaaa gttcgtgcgc
     8101 tggtggcggc attaggtggc ggtttctgga cgtggtggtt ctggcttggt gtcgccgggc
     8161 tggggctgat tgtgccaatg ttgctcaaac cgtgggtcaa tcgcagttcc ggcattcctg
     8221 ccgtgctggc ggcgtgtggg gccagtctgg tcggcgtgtt gatgctgcgc tttttcattc
     8281 tctacgccgg acagttgacg gtggcgtaag ccagaaaaga ggtggtttct ggacgtattc
     8341 cttcctgaag tcggttttct ggcgttgttg ttaagtctcg gggtcaacgt gttgaccccg
     8401 ttgacggcct ttgcgggagt gcggttgcgc tggcctgcca tgatgcgact cacttgcatc
     8461 ggcattctgg cgcagttcgc gctcctgctg ctcgcctttg gcgtactgac gtattgtttt
     8521 ctcatcagcg atttctcggt catttatgtc gcgcaacata gccatagcct gctgtcgtgg
     8581 gaattcaagc tggcagcggt gtggggcggc catgaaggtt cgctgctgct ttgggtgctg
     8641 ctgctttccg cctggagcgc gctgtttgcc tggcattatc ggcagcaaac cgatccgcta
     8701 tttccgctga cgttagccgt tttatctctc atgctcgccg cactgctact gtttgtggtg
     8761 ctgtggtccg atcccttcgt gcggatattt ccaccagcaa tcgaaggccg cgatctcaat
     8821 ccgatgctgc aacatcccgg tcttatcttt catccaccgc tgctttacct tggctatggc
     8881 ggtttgatgg tagcggcgag cgtggcgctg gcgagtctac tgcgcggcga gtttgatgcg
     8941 gccagcgccc gaatttgctg gcgctgggcg ttacctggct ggagtgcatt aacggcgggg
     9001 atcatcctcg gttcctggtg ggcctactgc gaactcggct ggggcggctg gtggttctgg
     9061 gatccggtgg aaaatgcctc tttattaccc tggctttctg ccactgcgct gctgcacagt
     9121 ttatccctga cacgccagcg ggggattttt tgccactggt cgctgttgct ggcgatagtt
     9181 actctgatgc tgtcgctgct gggcacctta attgtccgtt ctggcattct ggtttcggtt
     9241 catgcgttcg cactggataa cgtccgcgcc gtgccgttgt tcagcctgtt tgcactgatt
     9301 agccttgcgt ctttggctct gtatggctgg cgagcgcggg acggtggccc ggcggtgcgt
     9361 ttttcggggt tatcgcggga aatgttaatc ctcgctacgc tgttgctgtt ttgcgcagtg
     9421 ctactgatcg tgctggtggg aacgctttat ccgatgattt acggtctgct gggctgggga
     9481 cgcctctccg ttggcgcgcc gtattttaac cgcgcgacgt taccgtttgg tctgttgatg
     9541 ctggtggtga ttgtgctggc gacgtttgtc tctggcaaac gcgcgcagct tccggcgctg
     9601 gtcgctcatg cgggcgtgct gttatttgcc gcagggatcg tggtttccag cgtcagccgt
     9661 caggagatca gcctgaattt acagccgggt cagcaggtga cgctggcagg atacaccttc
     9721 cgttttgagc gcctcgatct gcaagccaaa ggcaattaca ccagcgaaaa agcgatagtg
     9781 gcgctgtttg accatcagca acgcattggt gaattaacgc cggagcggcg tttttatgaa
     9841 gcacgccgtc agcaaatgat ggaaccgtca attcgctgga acggcatcca tgactggtat
     9901 gcggtcatgg gtgaaaaaac cggagcggat cgttacgctt ttcgtttgta tgtacaaagc
     9961 ggtgtgcgct ggatctgggg tggaggattg ttgatgattg cgggcgcatt gttaagcgga
    10021 tggcggggga agaagcgcga tgaataaagg gcttctcacg ctactgctgt tatttacctg
    10081 ttttgcgcgc gctcaggtcg tagacacctg gcaattcgcc aatccgcaac aacagcaaca
    10141 ggcgttaaat attgccagcc agttacgttg tccgcagtgc cagaatcaaa acttactcga
    10201 atccaatgcg ccggtggcgg tcagtatgcg ccatcaggtt tatagcatgg tgtcggaggg
    10261 gaagaacgaa gtcgaaatca ttggctggat gaccgaacgc tacggagatt ttgttcgcta
    10321 taacccaccg ttaacgggtc agacgctggt gttgtgggcg ctgccagtgg tgttgttatt
    10381 gctgatggca ctgatcctct ggcgagtgag ggctaagcga tgaaacagcc tcaaataccg
    10441 gtgaaaatgc tgacaacact cacgatattg atggtattcc tctgtgtcgg cagttatctg
    10501 ttaagcccaa aatggcaggc ggtacgtgcg gagtatcagc gtcagcgcga tccgctacat
    10561 cagtttgcca gccagcaaac cccggaagcg cagcttcagg cattgcagga taaaatccgc
    10621 gctaatccac aaaacagcga acaatgggcg ttactgggcg agtactatct gtggcaaaac
    10681 gattacagca attcgctgct ggcgtatcgt caggcgttgc aactacgtgg tgagaacgct
    10741 gaactgtatg cggcgctggc gacggtgctt tattaccagg ccagccagca tatgaccgcc
    10801 cagacttgcg caatgatcga caaagccctc gcgctggact ctaacgaaat caccgctctg
    10861 atgctgctgg cttccgatgc gtttatgcag gcgaattacg cgcaagccat cgaactatgg
    10921 caaaaagtga tggatctcaa ctcaccccgg atcaaccgga cacagctggt cgagtcgatt
    10981 aatatggcaa aactgttgca gcggagatcg gattaatcat ctggtgatga agattatgtc
    11041 attgagctgc ataaaaaata atcgaatgaa catatgccaa aaataatcac taatcagtat
    11101 tattgcagat taaacaaata aaaatctttc cataacaaat ggttattcat taatcctgcc
    11161 ataaactcgc gttattatgc attaatgcag cgaaaagctc tgttgttaaa gggttgcgca
    11221 acataccgcg caaatgatac tgatcataag cgttaaaaaa atctacaaac caacgcaaca
    11281 caattcatgc cctggcagta tgtcacgttc tcgcgtttct gaacggggaa cggcgctcca
    11341 ttgaggaagt tattctgatg aaaaatataa aattcagcct ggcctggcag attctgtttg
    11401 ctatggtgct gggcattctc ctgggaagct acctgcacta ccatagcgac agccgcgact
    11461 ggctggtcgt caatttgctc tctccggcgg gtgatatctt catccatctg atcaaaatga
    11521 ttgttgtgcc gattgtgatc tccacgctgg tggtgggtat cgcgggtgtt ggtgatgcca
    11581 aacagctcgg gcgtattggc gcgaaaacca ttatttactt cgaggtgatc accaccgtcg
    11641 ccatcatttt ggggatcact ctggcgaacg tcttccagcc cggtgccggg gtggatatgt
    11701 cgcagctggc gaccgtcgat atctcgaaat atcagagcac tacggaagcg gtacaaagca
    11761 gttcccacgg cattatgggc acgattttgt cgctggtgcc gacgaacatt gtggcgtcga
    11821 tggcgaaagg cgaaatgctg ccgatcatct ttttctcggt gctgtttggt ctggggcttt
    11881 cttccctgcc cgcgacgcat cgtgaaccgc tggtgaccgt gttccgctcc atctctgaaa
    11941 ccatgtttaa agtgactcac atggtgatgc gttatgcacc ggtgggtgtg tttgcgctga
    12001 ttgcggtgac ggtggctaac tttggtttct cttctctgtg gccactggcg aaactggtgc
    12061 tgctggtgca tttcgccatt ctgttcttcg cgctggtagt gctgggaatt gtggcgcgcc
    12121 tgtgcgggtt aagcgtctgg atcctgattc gtattctgaa agatgagctg attctggcgt
    12181 actccactgc cagctctgaa agcgtactgc cgcgaattat tgagaagatg gaagcctacg
    12241 gagcaccggc gtcgatcacc agcttcgtgg tgccgaccgg ttactctttt aaccttgatg
    12301 gttcgacgct gtatcaaagt attgccgcta ttttcatcgc tcagctgtat ggcattgacc
    12361 tgtccatctg gcaggaaatc attctggtgc tgacgctgat ggtgacctcg aaagggattg
    12421 ctggcgtgcc gggcgtgtcg tttgtggtgc tgctggcaac gctgggtagc gtaggtattc
    12481 cgctggaagg tctggcgttt attgctggtg ttgaccgtat cctcgacatg gcgcgtactg
    12541 cgctgaacgt ggtgggtaat gcgctggcgg tgctggtgat tgccaagtgg gaacacaaat
    12601 ttgaccgcaa aaaggcgctg gcttatgagc gtgaagtgct gggcaaattt gataaaactg
    12661 cggatcaata attgaagatt gccggggata tccacccggc aatgtgtgaa tgcctgatgc
    12721 gacgcttgcc gcgtcttatc aggcctacct taaccgttgg ttaatttttc aaactcttca
    12781 cacccggtat caaacccttc catacagctc aggttcaacc agtgcagcgc cttttgcttg
    12841 ttcttctcga taaaaccttc ttcaccgttt aagaacatca ttcccgccca gtactcggaa
    12901 taaccggttc gggaaattgc agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg
    12961 gccttaatgc ccacgccgtt ggcgtaaatc aaaccaagca gcatttgggc atcgacggca
    13021 gagtcgttct ccagatcttc cgaggcgttt tctaacagcg aaatagcctt tggataatcc
    13081 ggtttacccg cttgagtatt taccagaata tgcgccagcg ttacttcacc ttctttactg
    13141 cccgcttgcg ccgctttctc tgcaagaact tttgcttgtg gatagtccag actgaccgga
    13201 ttggtgattt tgatctgcgc cagcagcgcg caggcatcag catcaccgct gtcggcggct
    13261 ttctgtgccc aatattcggc tttgctcaaa tcgccggagc taaaccagct gtcggcaaga
    13321 aaatattgtg cgcgtcggtc cccggcctct gctgccttta aatactggct gccaggctcg
    13381 gagtcgttgg cgtgggcaaa gaatgtcaaa aacaacatca atgcgataat ttttttcatt
    13441 ttgataatac gattgggtag ggagtaacca gtataaagag ttcgaatgga taaaaaaaca
    13501 gcctccgaaa ggaggctgta gaaaggactg tattacgcca gtgccgcttc gcgcaggcga
    13561 gttttcaact tgttgtactc gtcaatcacg tactgctcgg cggcgcgctg atcggcgatc
    13621 ggctcgacgc gcacggcgca gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt
    13681 tcggtaacca gctcgttaca ggcaccaatc caccactggt aggtcatgta aatcgcccct
    13741 ttgttcggac gatcgctgac ctgcgcacgg gtgataattt tgcctttacg cgagtgcacc
    13801 caaaccaatg cctcatcttc aatacccaga cgtttggcgt cttcggtatt gatttgtgcg
    13861 tagccaggtt catcagccag cgccgccagt gccgcacagt taccggtcat cgaacggcaa
    13921 gagtagtgac caacttcacg caccgttgac agtaccatcg ggtactcgtc ggtgagtttg
    13981 tcgattggcg ctacccagtc gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac
    14041 ttctctttaa acagataaga agtcccctga tcggcatctg aagtatcgcg gcaaggccac
    14101 tgaatgaagc ccagttcgcc cattttctcg taagtcgcac cgtagaaatc cgggcacaga
    14161 tgacgcaact catcccagat ctcctgggtg ttgttgtagt gcatcggata acccatacgg
    14221 gtggcgattt cactgatgat ttgccagtcc gttttcagat cccatttcgg ttcaactgct
    14281 ttgaagaaac gctggaagcc acggtcagcc gcagtaaaca cgccttcatg ctcgccccac
    14341 gacgttgacg gcaaaatgac atccgccgcc gacgcggttt tggtcataaa gatgtcctga
    14401 acgataacca gttccagatc ttcaaaggct ttacgtactg ccgacagctc cgcgtcagtt
    14461 tgcagcggat cttcgcccat aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc
    14521 ggcagctcgc tgatgcgata accggtatga gcaggcaggc tttccacgcc ccaggctttg
    14581 gcgaatttct cgcggttagc cggatctttc acgtactggt atcccggata cgtatccggc
    14641 agcgcgccca tatcgcaggc accctgcacg ttgttctgac cacgaaccgg gtttacaccc
    14701 gcatgcggct taccgaggtt accagtcagc atcgcgaggc tggtcagaga acgcacggtt
    14761 tccacgccct ggtagaactg ggttacaccc atgccccaca ggatggcggc gcttttcgcc
    14821 tgggcataca tccgtgccgc ctgacgaatc tcactggcgc tgacgccggt gatatcttca
    14881 accgactccg gcgtgtagcc ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg
    14941 gaagcgacga acgctttgtc gtacagattt tcttcaataa tgacatggcc catcgcattc
    15001 aacagcgcga tgttcgagcc gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt
    15061 tcaattttgc gcggatcgca gacgataatt ttcgccccgt tacgtttagc gttaattacg
    15121 tgattcgcca cgattgggtg ggaatcagcc gggttgtacc cgaaaacgaa cactaaatcg
    15181 gtattatcaa tttcgttaat agcattgctc attgcgccat taccgaccga ttggtgcaga
    15241 cctgcaaccg atgggccgtg tcagacacga gcgcagcagt caacgttatt ggtaccaata
    15301 acggcgcgcg caaatttttg cattacatag ttggtttcgt tacccgtacc acgcgaggag
    15361 ccggtcgtct ggatggcatc cggaccgtac ttctctttga tggcgctcag gcgctcggta
    15421 acgtaattca gtgcctcatc ccaggaaaca ggttcgagtt tgccgccacg ctggcgacgg
    15481 atcatggggg ttttcaggcg cggggtcagg atctgggtat cgttaatgaa atcccagcca
    15541 taataaccct tcagacacag ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg
    15601 acgattttgc cgttatcgac gaccaggttg attttgcaac ctgatgcgca ataggggcaa
    15661 accgtgacga cttttttcat cggtctcgct ccagttaatc aaatcacgca tacgcgctct
    15721 cgactacagt atgcatcttt tatgccacat tttatttggg gtcattccct gatattacgg
    15781 gcactattta ttcaaaactc tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa
    15841 cgaaataccg cgtgacagcc atcacgcggc agacatttta ttttttctcg acgacgggag
    15901 ccgcctgata cccgccgccc agcgatttca tcaactgaat gctttggatc acccggcggc
    15961 tgtccagcat cagtaatgac atctcttcgg caagtactgg caaccgggct tcagtggcct
    16021 gtaagcggct ggttaagccg cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg
    16081 ttgcttccac gcgttcagcc tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc
    16141 cgttgacggc aacgtcacgc accgcgttca gtactgactg gttgtaacgt tcaatcatca
    16201 tgttgctggc ggcgcgcgtg ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg
    16261 gcaatttcag acccgggatg aagttgaact ggcgactggt ttttttgaat aaggtatcca
    16321 ggtggatggc atcaaggccg aaaaacgctt tgatatcaaa gctcgggtag aacagcgccc
    16381 gcgcggaatc cacctgatct aatgacgcct gaacatacca gcgcatggct tgcagatccg
    16441 ggcgtctggc gagcaactca taagagagtg tcgccggaat gccggtctgg actcgtggta
    16501 atgccaccgg tttgatctcc ggcatatcgc tggcccccgc gccaatcaat gcacgcagag
    16561 attctcgcgt ttcggtgatt tgccctttga cggcagcaat ttgtttatcg accgccagaa
    16621 tctgtgcccg cgcgccgtgg aaaggcactt gtgcttccag accgtgcgcc actttactct
    16681 ggtgcgcttt caccgcgtaa tcaatcacat cgcgagtttg ttctaacaga tcgagcatct
    16741 gatagctggc ctgcatactg taataaagct gcgctacgcc cgtggtcagc gatagctcta
    16801 ctgctgcggt ttctgccagc gcggcattat gcgcgccaat ggcggcggca accgctgagc
    16861 gatgcacacc ccacaaatca agatccagtc cggcaaacaa acctactgtg gcttccgtat
    16921 agtacggccc gtccataccc agtgcgggcg catccatcgc ataagggctt aaaaagccgt
    16981 tcgccgagac gcgttggcgg ttgagcatcc ctaacgctgc gacctgtaat tgtgaaccgg
    17041 catctaacaa atcggcctgg gactgcgctt tttcttcccg cagtttcgct tcggcgaggg
    17101 tgtgtgaacc acttagcgtc cgttggatca gcgcatccag ctgcgggtca ttgagttgtt
    17161 tccaccactg cgcctgcggc cagccggagc tggcaagatg aatatcatcg gccagtttga
    17221 tttgttccgg tttgagctgt tgatgaggcg cagaatcctt gcgtaccaag gcacagccgg
    17281 aaatcagcgt cgtgctgccg agaatgctgc acaacagcag acgtgaaagt tgacgattga
    17341 tcattgcgtg gctccttgtg cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc
    17401 tggcaagcgg gcgaaaagct ggcagacgtg ttgctcctgt tttaataagg tgggcaggat
    17461 ggcctgcgag gcgggtgtct cttcaagcgt tatttgagga gaacggctga gtgcggttgc
    17521 cagaccggca gcgtattttt ccagggcgtc ggcaaactga cctgctctgt ccggctgtag
    17581 tgcgttatcc agcgcctgcg ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg
    17641 gccctgacga ataaccgttt gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag
    17701 ttgacgctcc agcgccacgc gttggcacat ctcttcacag gcattaaacg ccgcatgcag
    17761 accgatacga atttgcagat aagtgcgcag agccgtgact tcctgctggc gtgggatccg
    17821 cattacttta cttaacatac ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc
    17881 actttcaggc cagacaaagg tgtaaatcac cgcggacacc acggtaccaa tgatgatacc
    17941 cagggcgcga tcgcgaattt ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt
    18001 ggcgagcgcg aaggtgacca ccatctgtgt gccgatataa gaagagcgtt cagagctggt
    18061 ggcaatccat gcgcccaaca ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt
    18121 gtccagccag ggcatgacca gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc
    18181 cccgccaaaa cgcagcacca tcttctggta cgacgaaccg acatttggat tagagacgat
    18241 cacgcaggtc agcatacagg tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta
    18301 acagatcaaa caggcgagca atgtttttac cgcgtagcgt atatagtctg gattggtaaa
    18361 agcatcggcg accattgatg gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat
    18421 ctggcccagt tgtaacaacg tctggcagat attctccagg ttacattccc gtgccgccac
    18481 cgcttcactt tcactgatcc gccagtcgct ttgccagcac tgaccttcag caacggcatg
    18541 ctgcagcttg ttgatttctg aggctaattt ttgccgaaat tcaataattg cctgagaatc
    18601 agcaaaagag gtgggatcgt agcgattcag cgtcgagtaa atgtaggtta ccgttgccac
    18661 gcagctttgc caccatgcgc tttgagttcg ccagttggca tcgtccgcga gacaaaagac
    18721 attcagtttt tgcagcgcca gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag
    18781 gctgtctgtc aggtgactaa tggcatcatc aagccgatca ttaagcgcct gatgcatttg
    18841 cgaaatggca cgactgggaa accacagcac gccgattaac gtcatcagca aggttggata
    18901 gaggccaaca acgatacacc acagcgttaa gcgcacgacc acttccggat agtcgagcat
    18961 ggcggggaag gtttgcccgt aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag
    19021 ccgatgggtg cgcatcaaaa acatgcggcc catcaggatc ggcccggcga tgatcaatcg
    19081 gatcaacggt tcgccgtatg accatttgta gatcaaaaac aggctgccga tctccagcac
    19141 cgtggcaacc acaaacaaga tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc
    19201 gtaaaacagc actgccagcg ataacgccac aaaagggatc tcaaaagtca tcgagatcag
    19261 aatcaccagc aggcagccta cccagagttg cacgatctgc ggcacgcggc cgggtcgccg
    19321 ctcgcttaac tcttcatgaa agaacgccag cagcctgacc atcggtaatg gcagggagtt
    19381 gagcgcgctc attattgcgg ctcaagatta gcgactgccg aagcgccgat gcggaacatt
    19441 tccgggtcag gtttatcgac catgattttg accggaaaac gctgggcaac gcggacccag
    19501 ttaatagagc gagaaacttt cggcaggccg cccagcacca ggccgccgtc atccggtagc
    19561 acgccgtagc caatcgaatc cactttaccc tcgaaggttt tgccgctgtc actcatcagg
    19621 cgaatcgttg cgggtgtacc tgagcgaata tttttcagat cggtttcgcg gaagttggcg
    19681 atcacgtacc agtgacgagt gtcgattagg gtaaaaatag ggcgcatggc agaagcaaat
    19741 tgcccgacgg aggttttgag ggaaatgacc cggccatcaa acggcgcgcg aacggtcgcc
    19801 atttccagat gcagtttggt cagggcaata tccgcttcga ccgccgcacg ttgcgccacc
    19861 agcgcatcga cgccgctgac ggcgctggcg gctgactgcg cctgtaacaa tacggcatta
    19921 agatcggctt ctgcggcgcg ctgcgcagtt cttgcacggt caacgtcttc cgctgagaca
    19981 aaaccctctt tcagtaatgg ctcggtacgg cgtaatgtat ctgtggcctg tttcgcggcg
    20041 gcacgggctt tttctaccgt ggcattaatc gagtcggcac caaactgttg cgcatcaacg
    20101 ctacgctggg tgagcataat ttgcttatcc agcgccgcga gggaggcttc agctttcgcc
    20161 agattggctt cgtacgggcg cgggtcaatg cggaacagca aatcgccctg tttgactgcc
    20221 tggttgtcgg tgaccgccag ttctacaatg cggccgctga cttccggcac cacatcaatg
    20281 gtatctgctg acgcgtaagc gtcattagtt gatggcgcac tgtctacgcg ccagataacg
    20341 aaaacaaggg caaccagcgc caatgcaacc actaacagag cagggaattt actgcgagg
//
LOCUS       DAETQN010000074        20299 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.074, whole genome shotgun sequence.
ACCESSION   DAETQN010000074 DAETQN010000000
VERSION     DAETQN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.074"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..69
                     /locus_tag="K0W68_002685"
     CDS             <1..69
                     /locus_tag="K0W68_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBI3074208.1"
                     /translation="ILKTTDYLRIAQPELLNFRRTA"
     gene            complement(70..174)
                     /locus_tag="K0W68_002686"
     CDS             complement(70..174)
                     /locus_tag="K0W68_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074209.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            344..790
                     /locus_tag="K0W68_002687"
     CDS             344..790
                     /locus_tag="K0W68_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBI3074210.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(747..1568)
                     /gene="nimR"
                     /locus_tag="K0W68_002688"
     CDS             complement(747..1568)
                     /gene="nimR"
                     /locus_tag="K0W68_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HBI3074211.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            1665..2846
                     /gene="nimT"
                     /locus_tag="K0W68_002689"
     CDS             1665..2846
                     /gene="nimT"
                     /locus_tag="K0W68_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HBI3074212.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            2901..3248
                     /locus_tag="K0W68_002690"
     CDS             2901..3248
                     /locus_tag="K0W68_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBI3074213.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3270..3524)
                     /gene="yoaF"
                     /locus_tag="K0W68_002691"
     CDS             complement(3270..3524)
                     /gene="yoaF"
                     /locus_tag="K0W68_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HBI3074214.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            3707..4732
                     /gene="dgcP"
                     /locus_tag="K0W68_002692"
     CDS             3707..4732
                     /gene="dgcP"
                     /locus_tag="K0W68_002692"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HBI3074215.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            4765..4863
                     /locus_tag="K0W68_002693"
     CDS             4765..4863
                     /locus_tag="K0W68_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HBI3074216.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            4866..4940
                     /gene="yoaJ"
                     /locus_tag="K0W68_002694"
     CDS             4866..4940
                     /gene="yoaJ"
                     /locus_tag="K0W68_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HBI3074217.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(4999..5247)
                     /locus_tag="K0W68_002695"
     CDS             complement(4999..5247)
                     /locus_tag="K0W68_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBI3074218.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(5395..5577)
                     /locus_tag="K0W68_002696"
     CDS             complement(5395..5577)
                     /locus_tag="K0W68_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HBI3074219.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(5581..5940)
                     /gene="yeaR"
                     /locus_tag="K0W68_002697"
     CDS             complement(5581..5940)
                     /gene="yeaR"
                     /locus_tag="K0W68_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HBI3074220.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(6113..6751)
                     /gene="leuE"
                     /locus_tag="K0W68_002698"
     CDS             complement(6113..6751)
                     /gene="leuE"
                     /locus_tag="K0W68_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HBI3074221.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(6878..7801)
                     /gene="dmlR"
                     /locus_tag="K0W68_002699"
     CDS             complement(6878..7801)
                     /gene="dmlR"
                     /locus_tag="K0W68_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HBI3074222.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            7904..8989
                     /gene="dmlA"
                     /locus_tag="K0W68_002700"
     CDS             7904..8989
                     /gene="dmlA"
                     /locus_tag="K0W68_002700"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HBI3074223.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVAIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            9240..10850
                     /gene="yeaV"
                     /locus_tag="K0W68_002701"
     CDS             9240..10850
                     /gene="yeaV"
                     /locus_tag="K0W68_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HBI3074224.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGGAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            10882..12006
                     /gene="yeaW"
                     /locus_tag="K0W68_002702"
     CDS             10882..12006
                     /gene="yeaW"
                     /locus_tag="K0W68_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HBI3074225.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            12062..13027
                     /gene="yeaX"
                     /locus_tag="K0W68_002703"
     CDS             12062..13027
                     /gene="yeaX"
                     /locus_tag="K0W68_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBI3074226.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(13081..14196)
                     /gene="rnd"
                     /locus_tag="K0W68_002704"
     CDS             complement(13081..14196)
                     /gene="rnd"
                     /locus_tag="K0W68_002704"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBI3074227.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(14278..15963)
                     /gene="fadD"
                     /locus_tag="K0W68_002705"
     CDS             complement(14278..15963)
                     /gene="fadD"
                     /locus_tag="K0W68_002705"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBI3074228.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(16168..16749)
                     /gene="yeaY"
                     /locus_tag="K0W68_002706"
     CDS             complement(16168..16749)
                     /gene="yeaY"
                     /locus_tag="K0W68_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBI3074229.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(16789..17484)
                     /gene="tsaB"
                     /locus_tag="K0W68_002707"
     CDS             complement(16789..17484)
                     /gene="tsaB"
                     /locus_tag="K0W68_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBI3074230.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(17542..19452)
                     /locus_tag="K0W68_002708"
     CDS             complement(17542..19452)
                     /locus_tag="K0W68_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBI3074231.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            19584..19928
                     /locus_tag="K0W68_002709"
     CDS             19584..19928
                     /locus_tag="K0W68_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBI3074232.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         4935 a         5248 c         4949 g         5167 t
ORIGIN      
        1 attttgaaaa ccacggatta tttgcgcatt gcgcaaccag aactgctcaa tttccgccgc
       61 actgcgtaac tagcctgtcc gttcaataag cagaacggac aaaaccagca caacagcaat
      121 ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac atttgatcgt tcatagcatg
      181 cccccgggag atagctgttt tcacgttatt gttagcgtgc acaaatggca gtttgatgac
      241 aattcgcctg attttttatg caaaaaacgt aaagattttc tacttccttc ctgcagcaaa
      301 cgtaaagtaa gcaggcttat tattttttgg caaggaaacc acgatgtttg atgtcacttt
      361 gctgatcctg ctcgggttag ctgcgctggg ctttatcagt cataacacca ctgtcgccgt
      421 ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta agcacctttt tcccgtggat
      481 tgaaaaacag gggctaagta tcgggataat cattctgacg attggcgtta tggcccccat
      541 cgccagcggg acgctaccgc cctcaacctt gattcactcg tttctgaact ggaaatcgct
      601 ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt gggcgcggcg tgaccttaat
      661 gggcagccag ccgcaactgg tcgccgggct gctggtaggc actgttttag gcgtggcgct
      721 gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt ctggtttcgc tgattgtggg
      781 gaaacagtag ttaatcccgc aatatatcgg cctggcgttt gccccaggcc tttcttaaac
      841 atagtaataa aggccgttgt tgaatcatac cccagcgtat gagcaacttt ctgtacagta
      901 tcgcctttca ctaatccctg aagtgccata atcagctgta actgctgacg ccactggcga
      961 aaactcaacc ccgtctcttt tactatcagt cgcgcaagat tgcgttcact catcgcaaaa
     1021 aagccagccc attgccctaa tgacccccac tcgacgggcc ctttcgccat catctccacc
     1081 atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca gctgttgttg tggctgctga
     1141 gggagttcat caaacaggac ctgaattaat ctgcgggtca tcggctcagc acgctgagtc
     1201 gtcgtcctgt ttgccagggt taaaatcaat tcccggcata agggcgatat tttcaatgtg
     1261 caacatgttg tcggcattgt cactgcgctg ggttcgataa acagaaaaca gagttcagcg
     1321 ttagctgtca cttgattgct atgttccacc ccgcccggta tccaaaccgc atattgcggc
     1381 ggcaccatcc ataaagcatt ttccaccgta caggtaattg caccatgtaa tgctagtatc
     1441 agctgtcctt tgcggtgctg atgtacagga ctggttaact catccgttcc ggcgtgaata
     1501 caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat agccattgag attcaacctg
     1561 tgcatcattt tgtccgaatt tagcgataat ttgtcatttt agcttgattc aacataacaa
     1621 taaaaacggt aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt
     1681 cattaagcgg caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat
     1741 tacgcgtcac ctttatcggc gcagcaccgt tactggatac gattcgttcc gcttactcgc
     1801 tgacgacagc gcaaaccggc ttattgacca ccctgccatt attggccttt gcgctaatct
     1861 cacctttggc caccccggta gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt
     1921 tacttttgat ctgtgctggt atcgcaattc gctctctccc ttcgccttac ttattatttg
     1981 gcggtacagc ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa
     2041 ttaaacgcga tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg
     2101 gagctgcagc ggcactggga tcggctatga tagtgccgct ggctttgaac ggttttggct
     2161 ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc
     2221 cacagtggcg aagccaacaa catgcaaatt tgagtacctc gcgcgcctta catactcggg
     2281 gtatctggcg ttcgccgctt gcctggcagg taacattgtt tcttgggatc aactcactga
     2341 tctattacgt gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag
     2401 cacaggcggg ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga
     2461 tccctctttt cttacatcat gtgaaagatc agcgtggtat tgcagcgttc gttgccttga
     2521 tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc gcacgcgatc acctggactc
     2581 tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc
     2641 gggcaagttc tgcgcatcag gcggcggcac tctcggggat ggcacaatcc gtcgggtatt
     2701 tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca cgatgctaac ggtgactggt
     2761 ccgtaccact gatgggtgtt gcaatacttt cactactgat ggcgattttc ggactttgcg
     2821 ccgggagaga caaagaaatt cgctaatatc cggtgctata gtgacgtgac aaatcatgcg
     2881 tgaaagggag aacaaacacg atgaatattc agtgcaaacg cgtttatgat ccggctgaac
     2941 agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc gcgcggtatc aaaaaaaccg
     3001 atttagccct tgatgagtgg gataaagaaa tcacgccgtc aacggaactg cgcaaagcct
     3061 ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca atatcttgca gaactggcgc
     3121 aacacgagca agaaggaaag cggctggcgg acatcgccaa aaaacagccg ctgaccctgc
     3181 tctacgcagc aaaaaacacc acgcagaacc atgcgctggt gctggccgac tggctacgta
     3241 gcttgtgatt ttagtacagc atccggcggt tatttttcac cagccggatg gtcccgtcgc
     3301 cacaatgccc attcatcaat cgtttcaccg cccggtaatt tgcaattgtt gctgacccct
     3361 tgcgctgtcg acactggaat gagcgtcccg cccttctgct ggcaatagac cgacgccgga
     3421 tttgccatac caatctgcgg cggtttaggt acttctggct gagaagaggt tgaacaacca
     3481 gccaggacca gcaagcaagg caaagcaaaa ctgataattt tcatttattg atctcactca
     3541 tttatctacg cttagagtat cgcggtatcg ttttgttttg cagcactatt tttattacat
     3601 tcactcaaaa catattacgt cttgtttcat ctttgttggt gatgttttat catgcctgca
     3661 aagattaaat aatcagcatt tacccgccgt atcctggagt tgtttcgtgt cagatcagat
     3721 tatcgcccgc gtctcgcaat cccttgccaa agaacagtca ctggaaagtc tggtccgaca
     3781 gcttctggag atgctggaaa tggtcactga tatggaatca acctacctga ccaaagtgga
     3841 tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac agccagaaaa tgcacatccc
     3901 ggagaatttt accgtctcgt gggattactc gttatgcaaa cgcgccattg atgaaaactg
     3961 ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt attgcggcac gcaatcttgg
     4021 catctccaca tttctgagca caccaattca cttaccggat ggatcattct atggcacgct
     4081 ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc gcggaacagg ttttacagtt
     4141 attcgccgga ctgattgcac aatatattca aaaagaggca ctggttgaac agttgcgcga
     4201 agccaatgct gcgctgattg cgcaatcgta taccgactcg ttaaccgggt tgccgaatcc
     4261 gcgggcgatt tttgaaaatc tgacgacgct gttttctctc gcccggcatc ttaaccataa
     4321 gataatgatc gcgtttatcg atctggataa cttcaaatta atcaatgatc gttttggtca
     4381 taatagtggc gatctgtttc tcattcaggt tggcgagcgc cttaatacgc tccagcaaaa
     4441 tggcgaagtt attggtcgtc tcggcggtga tgagtttttg gttgtttcac taaacaacga
     4501 gaatgcggat atttcgtcgc tgcgagaacg cattcagcag caaatacgtg gagaatatca
     4561 cttaggtgat gttgatttgt attatcccgg tgccagtctt ggcatagtag aagtcgatcc
     4621 tgaaacaacc gatgcagaca gtgccctgca tgctgccgat attgcgatgt atcaggagaa
     4681 aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg ccacattgct gaggcgtatt
     4741 cacatccttt tgattggtga taacatgcga atcggtatta tttttccggt tgtaatcttt
     4801 attacagcgg tcgtattttt agcatggttt tttattggcg gctatgctgc cccgggggca
     4861 taaagatgaa aaaaacaacg attattatga tgggtgtggc gattattgtc gtactcggca
     4921 ctgagctggg atggtggtaa cgtcacctct aaaaaatagc aaaggctgcc tgtgtgcagc
     4981 ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag ataaatagca cgacaatcgc
     5041 accaataacg gcaaccacga agctgccaaa attgaagcca tcgactttac caaagccaaa
     5101 cagcgtgctg atccatccgc cgactacggc accgactatc cccagcagga tagtcataaa
     5161 gaatccacca ccatctttac ctggcatgat ccacttcgcc agaataccgg caataagccc
     5221 aaaaataatc catgacagaa tgcccattgt ttcctcactt atctgttttg cattagcggg
     5281 ttagtcgctg ataaaaagca tagcacaaca tcgggagggc aagatttgtg acgagcatca
     5341 cggaggattt ttttgcgatg gcgcagaaat tgcgccatca acgatcagtg ataattacca
     5401 accacaaaca tcatgttcgt tttccgtgtc ataagaacgc acggtattca ccagatcttt
     5461 tatcacttca gccgccactt ctggcaccag caaagtcatc ggcgtctctg tttcataatc
     5521 gacagaaacg ccattactgt tattggtgac ggtcacggta tacgttgctt tgcccatgat
     5581 tcatttcccg ttatgaatga ctttccgttg ttgcgcacct tccatcagga cttcaggagc
     5641 cacgaagaag tcaatgttga aataagtatc gtcagtcatg gcttcaatgt tgtgccactt
     5701 ttctggaggg aacacctcaa actgccccgc ttcgataagg atcacctgat caggctctgc
     5761 actgtgttca tcagcgtagc cgagatattt gaccgcccca tgcataacgg aaaggcgtgg
     5821 gtaaaccccc gggcgcgttc ctttatcaag atgacgttcg aatattccgg caggcgcagt
     5881 ttgtttattc cagaaaggcg ttgagcgcgt atgaatataa ttctgtggga tttgcagcat
     5941 ccttttccct ccttcggtga atgcgctgaa aacggtttat tccagccgtt tcagggtacg
     6001 cctgataatt tgcattttaa ataccattta ttggttactt tttagcacca tatcagagaa
     6061 aaatcaggga ggattacaag tgggaagccc gcgacaacgc gggctgaatg tatcaggatt
     6121 gcagcgtcgc cagtcgggcg gcgaaaccca cgaacatcaa accaatcagt gagttgccaa
     6181 ctttagccag tttctttttg gtacgtatgt actgcgtgac aaaagcacca gaaataatca
     6241 ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt cgccagaata aagaatgaaa
     6301 ttcccgtatg tggggcatta acatcgataa actgtacgaa aaacgacaca tagaacaaaa
     6361 tggctttcgg attagtcagg ctcaaaatta acgcgcgttt aaaaatggcc ccgtattggg
     6421 gctcatcgga tttggcctcg ttatttttac ccttcagggt cgcgtagaga attttactcc
     6481 ccagatagag caaataaaac gcaccaagat aacgtacaat gttgaataat atcggggtgg
     6541 tcttaattaa tgtcgccact ccagcccatg ccagaaacat caatactgca tcgccaataa
     6601 atacaccgca ggccgcaaga taaccgcctt tcataccgct actgacgcta tttttgagta
     6661 caaacagggt atttggccct ggcaccaaca caataaaaat ggccccaacc agataggtcc
     6721 agtaattcag aaccccgtat tcagcgaaca cattaacctc tttaattatc tttcgatcat
     6781 gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc ttagaaagga gaaaatcagg
     6841 ttttaacccg atatcaaccc gataattgaa taattaatca ggcatgctcc agtgaaaaat
     6901 tcgggtagtg ctcggcaaaa tactggcgta aaaattctat cgttatccgc actttcgctg
     6961 acgtcgccag ccttgaaaca taaacggccc agacgttcgc tggctggtaa tattccggta
     7021 gcacttgcac taaatgacca ctggcaatgt tttcgctaac atcccaccag gagcgcagag
     7081 caatcccctg cccatcgaga caccactgat ggacaatttc tccatgatta gacgacagtg
     7141 ggccggtgac tttaatcgca tggtggcctt ctttattgcg taattgccag acaccaaaag
     7201 gatgatcgcg ctctttaatg accaggcacg gcaatgcgga aagatcggtt aaatgtttcg
     7261 gcgcgccatg ttgcgcaata aattccggcg acgcgcagag aatgcggtaa ttagtcgcca
     7321 gtttgcgggc gattaaatta ggggcgatgt catcgccaat gcgaatatcg agatcaacac
     7381 cttcattgac caaatcgacc aaccggtctt ccacatcaaa acgtaattcc agttgcggat
     7441 acgccttagc cagcgctgat aacgccggag ccaccacctg tcgtccaaaa ccaaagctgc
     7501 tgataatacg cagcatcccc tgcggcacct gacgcacgtc agaaagttcg tccatcatct
     7561 gaccgacatc ctgcaaaatc cgctgcgccc attcataaat ccgctctcct tcttcggtaa
     7621 tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt tagcgtttgc tccagcaagg
     7681 cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc ttccgccacg gcggcaaaac
     7741 cagcccggcg agccaccagc ataaagacgc gcaaatcgtt cagcagcggt aaattattca
     7801 tgattcgtgt tttatgtttc accagttacg gggattaatt ccttttcagt caattatagg
     7861 atggtgatgt tgtcaatttt gatggtcagg aagtgagaac ccaatgatga aaacgatgcg
     7921 tattgctgcg atcccgggag atgggattgg caaagaagtc cttcctgaag ggattcgcgt
     7981 attacaggct gccgctgagc gctggggctt cgacttgagt tttgagcaaa tggagtgggc
     8041 gagctgcgag tattacagcc atcacggtaa aatgatgccg gacgactggc atgagcaact
     8101 tagccgtttc gacgccatct attttggtgc cgtcggctgg ccggataccg ttccggacca
     8161 tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa ttcgaccagt acgtcaacct
     8221 gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg gcgggaaaac agcctggcga
     8281 catcgatttt tacgtggtca gggaaaacac cgaaggcgaa tattcctcgc tcggcggtag
     8341 agtgaatgaa ggtacagagc atgaagtcgc cattcaggaa tcggtattta cgcgtcgtgg
     8401 tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa agccgcccac gtaagacgct
     8461 aacttctgcc actaaatcaa acggtttagc catcagcatg ccgtactggg atgagcgagt
     8521 ggaagcaatg gccgagaatt acccggagat ccgctgggac aagcagcata ttgatattct
     8581 ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg gtggtggcgt ccaatttgtt
     8641 tggcgatatc ctttccgatc ttggcccggc ctgcaccggc accattggca ttgccccatc
     8701 cgccaacctg aatccggaac gcactttccc gtcgctcttc gagcctgtcc acggttccgc
     8761 gccggatatc tacgggaaaa atattgctaa ccctatcgcc acaatttggg ccggtgcgat
     8821 gatgctcgat tttctcggca atggcgatga gcgtttccag caagcgcata acggtattct
     8881 ggcagcgatt gaagaagtga ttgctcacgg gccgaaaaca ccggatatga aaggcagtgc
     8941 caccacgcca caggttgccg acgcgatttg caaaattatt ttgcgttaag gtcaaaccag
     9001 tttatttgaa ccgcgtcact gacgcggttt ttttattcgt tctttgcagt taataacctg
     9061 cgtcatttca ccttttattg tttccgtttc gtgttttatg gctttccgta ttcttaattg
     9121 tttaatttat gtaacatgca aataatcaat ttgttaaaca caacatacaa ctcattaccc
     9181 tgcaaataat tacaactggc aaaagccaga gacgggcttt tatgcccagg agatgactca
     9241 tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag catcagcctg gtggccattc
     9301 ttttcatcgc agctgcatta agccttttcc cacaacaatc ggccgacgcg gccaacacaa
     9361 tatacacttt tgttacgcgt acgttaggtt ccgccgtaca ggtattggtt ttgctggcaa
     9421 tgggactggt gatttattta gccaccagta aatacggcaa tattcgtctt ggcgaaggaa
     9481 aaccggaata cagcacgctc tcctggctgt ttatgtttat ttgtgccggt ttaggttctt
     9541 ctacgcttta ttgggggggt gctgaatggg cctattatta tcaaacgcct ggattaaata
     9601 tcgcaccacg ttcacaacag gcactcgaat ttagcgtccc ttactccttc ttccactggg
     9661 gcatcagcac ctgggcaact tatacgctgg cctcattaat catggcttat cactttcatg
     9721 tgcgtaaaaa caaaggtctg agtctttccg gcattattgc cgcgattacc ggcgttcgcc
     9781 cgcaaggccc atggggaaaa ctggttgatt tgatgtttct gatcgccact gtcggcgcac
     9841 tgactatttc ccttgttgtc accgcagcaa cctttactcg tggactttcc gcgctgaccg
     9901 gtttacccga taacttcacc gtgcaggcat ttgtgatcct gctttccggc ggcatttttt
     9961 gcctaagctc gtggattggt atcaacaacg gtttgcaacg tctgagcaaa atggttggct
    10021 ggggcgcgtt cctgctgcca ttactggtgc tgattgtcgg tccaaccgaa tttattacca
    10081 acagcatcat caatgccatc ggcctgacca cgcaaaactt cctgcaaatg agcttattca
    10141 ccgatccgct tggcgatggt tcatttaccc gcaactggac cgttttctac tggctgtggt
    10201 ggatctcata cccccctggc gtagcaatgt ttgtcacccg cgtttcccgc ggtcgtaaga
    10261 ttaaagaagt tatctgggga ctgatcctcg gcagcaccgt cggttgctgg ttcttcttcg
    10321 gcgtaatgga aagctatgcc attcatcagt ttatcaatgg cgtaatcaac gtcccacagg
    10381 tgctggaaac actgggcggc gaaacagctg tacagcaagt tctgatgtcg ttgccagccg
    10441 gtaaattgtt cctcgccgca tacctgggcg tgatgattat tttccttgcc tcgcatatgg
    10501 atgcagtggc ctacaccatg gcggcgacca gtacgcgtaa tctccaggaa ggtgacgatc
    10561 ctgaccgtgg gctgcgtctt ttctggtgcg tggtgatcac tctgatcccg ctttccatct
    10621 tgtttaccgg tgcttcgctg gaaacgatga aaaccaccgt cgtgctcaca gcccttccct
    10681 tcctcgtcat tttactggtg aaagtcggcg gatttattcg ctggctgaaa caggattatg
    10741 ccgacattcc ggctcatcaa gttgaacatt atctcccgca gacaccggtt gaagccctgg
    10801 aaaaaacacc agtgctccct gcgggaaccg tattcaaagg cgacaactga gcgccatcat
    10861 cctaacgata aaggtatccc tatgagcaat ctgagccctg actttgtact acccgaaaat
    10921 ttttgcgcta acccgcaaga ggcgtggacc attcctgccc gtttttatac cgatcagaac
    10981 gcgtttgaac acgaaaaaga gaacgtcttc gccaaaagct ggatttgcgt cgctcacagc
    11041 agcgaactgg cgaatgccaa tgattatgtg acgcgtgaga tcattggcga aagcatcgtg
    11101 ctggtacgcg gtcgtgataa ggttttgcgc gcgttctata acgtgtgtcc gcaccgtggt
    11161 catcagttgt tgagcggtga aggaaaagca aaaaatgtga ttacctgccc atatcacgca
    11221 tgggcattca aactcgatgg caatctggcc catgcacgta actgcgaaaa cgtcgccaat
    11281 ttcgatagcg acaaagcgca actggttccg gtgcgtctgg aggaatatgc cggattcgtc
    11341 ttcatcaaca tggaccccaa cgccaccagc gtagaagatc agttacccgg tctgggcgcg
    11401 aaagtgctgg aagcctgccc ggaagtccac gatctgaaac tggcggcccg ctttaccacc
    11461 cgcacgcctg ccaactggaa gaacattgtc gataactatc tcgagtgcta tcactgtggt
    11521 ccggcgcatc caggtttctc cgactccgta caggttgatc gctactggca caccatgcac
    11581 ggtaactgga cgctgcaata cggtttcgcc aaaccgtccg aacagtcgtt taaatttgaa
    11641 gagggtacgg atgcggcatt ccacggtttc tggctgtggc cgtgcacgat gctgaacgtc
    11701 accccgatca aagggatgat gacggtcatt tatgaattcc cggtggattc tgaaactacc
    11761 ctgcaaaact acgatattta cttcaccaat gaagagttaa ccgacgagca aaaatcgctg
    11821 attgagtggt atcgcgatgt gttccgtccg gaagatttac gtctggttga aagcgtacag
    11881 aaagggctga aatcgcgtgg ctatcgtggt caggggcgca tcatggccga cagtagcggt
    11941 agtggcattt ccgaacatgg tatcgcccat ttccataatc tgctggcgca ggtgtttaag
    12001 gactaatgac atcggcggcg gtattttccg ccgctgggct gatttttgat ggagtacagc
    12061 aatgtcagac tatcaaatgt ttgaagtaca ggtgagtcag gtggaaccgc ttaccgaaca
    12121 ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg ctgcctgcgt tcaccggagg
    12181 aagtcacatc attgtgcaga tgagcgatgg tgataaccag tacagcaatg cgtattcact
    12241 actgagttcg ccgcatgaca cctcttgtta tcagattgcc gttcggctgg aggaaaactc
    12301 gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg ggcgatcggt taacgatttc
    12361 aacgcctaat aacctgtttg cgctaattcc ctcagccaga aagcatctgt ttatcgcggg
    12421 cggtattggt atcacccctt tcctgtcgca catggcagag ttgcaacaca gcgacgtcga
    12481 ctggcagcta cattactgct cgcgaaatcc agaaagttgc gcatttcgtg atgagctagt
    12541 ccagcatccg caggctgaga aagtccattt gcatcattcg tcaaccggaa cacgactgga
    12601 attagcgcga ttattggcgg atatcgaacc tggcacacac gtttatacct gtggccccga
    12661 ggcgctaaat gaagcggtaa gaagtgaagc tgcgcgtctg gacatcgccg ccgatacgct
    12721 gcactttgag caatttgcta tcgaagacaa aaccggcgat gcctttaccc tggtgcttgc
    12781 ccgttccgga aaagagtttg tggtgccgga agagatgact attttgcagg ttattgaaaa
    12841 taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg gtatgcggaa cctgcgaaac
    12901 agcaatactg gaaggtgaag ctgaccatcg ggatcagtat tttagcgatg aagagcgtgc
    12961 cagccagcaa agtatgttga tctgttgttc gcgtgcgaag ggtaaacgcc tggtgttgga
    13021 tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc cagtccggct tcggaagatt
    13081 ttactgcgga tattcctgca ataaattgtg taatgcttcc gccatcagct caccacgcca
    13141 gccggaaatc agctccggca aattgttctg cggtttcagt ttccagtgcc agttcagcag
    13201 ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg atcttatgcg tttcgctcac
    13261 gtcagtaatc agcgacttaa tcgctttaaa cgctttacga taacccggca tgtccatcag
    13321 gttaagcatc ggctgcggta aggcctcttc cggcaatgtc tgcgcttttt ccaccagcgc
    13381 cagcagcgtt ttaccgtgaa agcggatttc gctaccggat aaacccaggc tgtccagttc
    13441 gcctaaactt cccggcatat aacgcgcgac cgaccacaaa tgctcttcac gcacgacaaa
    13501 gttcaccgcc agatcacgct cacgagcctt gcgcagtcgc cagtcggcta acagttgcag
    13561 acaggccagt tggcgtgtgc gtaattgcca ggcattggtg atatcacgcc aggcatcttc
    13621 cggcgcaacg acttcctgac gacgcatttg catcaggcgg cattcatcca gcgccgcagg
    13681 tagccagccg gaggcctccg tttctaccat aagcttggcg gtgatcggta acagatacca
    13741 gacatccgcc gctgcgtatt cacactgacg ttcggtcagc ggtctggcca gccagtcggt
    13801 gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct tccaccatgg aagcgaaacc
    13861 ccatgacatc gggcgtccgc agaaggcagc aaggatttgc gtgtcaatca ggggttgtgg
    13921 taattcgcca aagacattga ggaacacttc cagatcttca ctgcctgcat ggagaaattt
    13981 tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac cagtcggtga tcccgagagg
    14041 atcaattagc gccagatgct caccatcgaa aagttgaatc aaccccagct gcgggtaata
    14101 agtgcgcgta cgaacaaatt cagtatcctg ggctatcgcc ggaaagacac ggacggcttc
    14161 acacaaagaa gccagcgcat cgtccgtggt aatcatttgg taattcaaat tgtattctct
    14221 ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct gacgactgac ttaacgctca
    14281 ggctttattg tccactttgc cgcgcgcttc gtcacgtaat tctcgtcgca aaatttttcc
    14341 gacgttagat ttcggtaact catcacgaaa ctccaccagc ttcggtactt tgtagcccgt
    14401 gagctgacgg cagcaaaagg tcaccagtga ctcttcggta agcgatggat cttttttcac
    14461 tacgaagatt ttcaccgctt caccactgga gccggaaggt acgccaacag ccgcgacttc
    14521 ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg ttgggataga cgttaaaacc
    14581 ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc aggaaccctt cttcatccat
    14641 caccgcgatg tcgccggtgt gtaaccagcc atttttgatg atctcatcgg tagcatcggg
    14701 acgctgccag taacccagca tcacctgcgg tcctttgaca caaagctcac ccggttgacc
    14761 cggtggtact tcattatcgt cgtcatccac cagtttggct tccgtcgacg gcaccggcaa
    14821 accgatgcta ccactatgat aatcaatatc atatgggtta acgctgacca gcggcgcaca
    14881 ctcggtaaga ccatagcctt ccagcagata ctgtcctgtc agtttcaccc aacgctctgc
    14941 caccacttgc tgcactggca tcccaccgcc tgcggaaaga tgcagactgg agaaatccag
    15001 ctgctggaac tctttattgt tcagcaacgc attgaacaag gtgttaacgc cagtgatagc
    15061 ggtaaacgga tatttcgcta actcttttac caaccctgga atatcgcgcg ggttagtgat
    15121 aagcaggttc tgcccaccca gttcgataaa cagcaggcag ttaatggtca gggcaaaaat
    15181 gtgatacagc ggcagtgccg ttactaccag ttctttacct ggatgcagca acggcccata
    15241 ggtggcgttg acctgttcca ggttcgccag catattgcgg tgagtcagca tcgcgccttt
    15301 cgccacacct gtggtgccgc cggtgtattg caggaaggct aaatcttccg gcaccagttc
    15361 gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg ctacgaaatg aaatggcatc
    15421 tggcagatgg tatttcggca ccaaacgctt gatgtattta acaacgaaat tgactaccgt
    15481 gccttttgcc gtagatagct gatcgcccat acgggtcaga attacgtgct gaacggcggt
    15541 tttatcaacc actttttcca gtgtgtgagc aaagttagac acgataacaa tcgccgatgc
    15601 gccgctatcg ttaagctgat gctcaagctc acgcggggta tacaacgggt taacgtttac
    15661 gacgatcatc ccggcacgca aaatgccaaa cagcgccacc ggatattgca ataaattagg
    15721 catcatcaac gcaacgcgat cgcctttctt cagccccaac ccttgttgca aataagcggc
    15781 aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatt acctccccca tattcacaaa
    15841 cgcaggctga tcggcgtagc ggacgaccga ctgctcaaac atatctatca gagattgata
    15901 acggtcaggg ttgatctccg tcggaacatc cgcgggataa cggttaagcc aaaccttctt
    15961 caattcttca cctctaaaat gcgtgttcgt cgtcatcgca accccaagtg ataaacatgt
    16021 tgttaacata acattaactc atcttaccag cttgataatt agacaacgaa aaggttgcga
    16081 agcgcgtcac tatttatttt tatagttgcc gtatgaacgc agaaacagcg gaccagccgc
    16141 tgtttctttt tctttaaaaa caacttgtta ctcggttaca attgtttcaa ctcttgcagg
    16201 gccaggatta taccaacccc atccgccgta accatagggc cagccgcgtc caccataaaa
    16261 ccatggatca attggctgcg gcggcataat cacctgttga gtgatatgcc agcgtttgta
    16321 acctgttacc tgcataacca taaatttata tggtgcatta tttactttgc catccactgt
    16381 gccagtgatt ggcccgacaa ccgtcaccag ttgtccacga aaatcgaccg gatcaagaaa
    16441 accgttcaca tcggcataaa tgcgcccgcg tgaaggttct cccaaggttg gtcttgctcc
    16501 gctgtccagc ggtacggtag caatttccag gcgggttttc ccttgctggt tttgtaccac
    16561 aaccactttg ccgccaaagc gcgcctcctg accaacatat aaatctggcg cattcatcac
    16621 gcgtaccaga tcttgctgtg gtgttggact gctgccttta atggcgtccg gcacagtgac
    16681 acaaccgctc agcattagcg caaacgtacc tgccagtatg cctttgataa cattcttttg
    16741 aaccgccatg atgcgactcc tttttttctc agggcatact cttaagattc attctttgcc
    16801 cggaagtttc ttccatgcga cgttgttacg taaataaacc ggttcggcat gttccaccgc
    16861 caccgtttta ccctcggcaa acatctgaca cgcaatcggc agcatatctt ccgccgctgg
    16921 cagtaaaact tctccgtcgc gcaaaaccag cccgctctct ttacggagat ccggccaggc
    16981 ttgccagccc gtccctaccg ttacccattc gccggaaagc tgttgcattc gttcatgaac
    17041 gagttcgggt ttgagtacgg cttcggtttc ttcaccgcgc cagataccgt tttcatcacg
    17101 ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca atggctgcca gcacgcgggt
    17161 tgcgccgttt ttgcgccacg ccccctgcgc catcgtcatt agcgtagaga caccaatcat
    17221 cggtaactcc gcgccaagcg ccagcccttg cgcaatacca ataccaatgc gcacgccagt
    17281 aaagctaccg gggccgcgcc cgtaagccag agcgtttatg tcagtcaggg aagttccgct
    17341 ggtggtcagg atatcctgca ccatcggtaa gattcgttga gtatgttcac gagggcaaag
    17401 ctcaaaatga gcgttgacat taccgtcgtt ccacagagcg acagagcaga cctctgtcgc
    17461 ggtatcgata gccagaattc gcatgggtct tcgtgcttag atcaataaaa aggcgcgcat
    17521 cataccatac tccgtaacaa attacctgga ggatggtatc gcaaggaaac gaaccgcacg
    17581 ggcaatgtca cgggtgcgtg gcgcgggcgg cagactggcg agaaacgtcg cgccgtaagg
    17641 acgcatcacc agccgattgt cacaaattac cagcacgccg cgatcgtcgg cgtcgcgaat
    17701 cagtcgccct actccctgct tgagagtaat gaccgcatcc ggcagctgca cttcatcgaa
    17761 cgggtcgcca ccgcgcaaac gacaatcttc catgcgcgct tttaacagta gatcatccgg
    17821 cgaggtaaac ggcaatttgt cgataattac caatgacaat gtatcgccac gcacgtccac
    17881 cccttcccag aaactactgg ttgccacaag aagcgcgtta ccggcgctga caaattgctg
    17941 caacagttgc cctttgctgg tttccccctg caacaaaacg ggaagcgtca tggtagcgcg
    18001 gaactgctcg gccagatcgc gcatcatggc atgcgaggta caaagcataa aacaacgacc
    18061 gttgttagct tcgatgatcg gtcgcagcat tgccgccagt tgccgtgcac aacccggttg
    18121 attggtttgc ggcaggttgc gcggcacaca gagtaacgcc tggcggctgt aatcaaacgg
    18181 gcttggcaat agcaacgact ccgcctgttc tatcccaagc cgcgaggtga aatgatgcag
    18241 atcgtcgttc accgacagcg ttgctgaggt gaagatccag ctaccgggct tttgcgccat
    18301 taactctttg aatttatccg ccacgctgag cggcgtgaga gccagagtaa aatggcgcga
    18361 agtgcattcg taccagtagc tgtagcccgg ctgattgatc tcttttagcc gcttcagccg
    18421 tgtgcgatac aacgtggcgc gctcaaatgc cgcatccagc aaggcggaac gccccagtga
    18481 cagtttcgcc acgtcataac aaagttccag ggtgtcatcg agcagtaaaa atgcccgctg
    18541 aatttgcgga ttagctaaca gctcacgcag gttaccgcga taacctggct caccgagttg
    18601 cagacgaaaa tcctgtgcac tctgggcaag acgatcagcg cacttttgta actgctgggt
    18661 gtcttttaat tcggtgcggt aggcgatggt gatgtctttt gccaggtcga gcagttgtcg
    18721 actggagagt gactgaccaa aatactggct ggcaatgtcc ggtagctggt gggcttcgtc
    18781 gaagatcatg acgtccgctt cagggatcaa ctcgccaaat ccactctctt taaccaccat
    18841 atccgccaga aagagatgat ggtttaccac caccacatcg gcgtccatcg cttttttacg
    18901 tgctttgacc acaaagcaat ctttatacat cgggcagtcg ctgccaagac agttgtcgtt
    18961 ggtgctggtg accagcggcc acgcctgtga atcttccgcc acgctgacgc aggtgctgat
    19021 atcaccatcg actgtttgat tagaccagga gcgcagcagg atcacatcgc ttaagatttg
    19081 taccggcaga tcgccccccg ccagcgcctg ctgttcgaga cgttcgaggc agaggtagtt
    19141 tgagcgccct ttcagcagcg ccacgttgcc cgtatatttc aatgcctttg agactgttgg
    19201 caaatcgcgg ctgtagagct gatcctgcaa cgcttttgag ccggtcgaga taatgacttt
    19261 ctttttcgcc cgcagcgcag gagccaggta agcgtaggtt ttgcccgtac cggttcctgc
    19321 ttccaccacc agcggctggc ctttttctat cgcctgggtg acggctaccg ccatctgtcg
    19381 ctgtggttct cgcggcttaa agcctggtat cgctttcgcc agctgaccgt ctggtgcaaa
    19441 atcgtccgtc acactacccc ctgttgattt gaacagggat tatgtcagga tgagggcgct
    19501 ttcgccagtt gaagtggtga cggcaacctc acattgtggc agtctttgca gcacgaaaat
    19561 ggaagtataa tgaggacaaa atgatgacta tcgttcgtat cgatgctgaa gcccgctggt
    19621 ctgatgtagt aatccacaac aacacgctct actacactgg tgtaccggaa aacctcgacg
    19681 ccgatgcctt tgagcaaacc gccaacacgc tggcacagat tgacgccgtg ctggaaaaac
    19741 agggcagcaa taaatcgagc attctggatg ccaccatttt cctggccgat aaaaacgact
    19801 tcgcggcgat gaataaagcg tgggatgcct gggttgtcgc gggtcatgcg ccagtgcgct
    19861 gcacggtaca agcgggtttg atgaacccga agtataaagt tgaaattaag attgtggctg
    19921 cggtgtaagc tttatcgaag caaaataagt cagacgataa tttatcgata atactggtcg
    19981 gttttacata aatcgaccag agaataagat attactgttg atgcttctgc taattcatta
    20041 tttatattta taatttcaat tttatctata aaactaacta tataaataaa cataaccatg
    20101 ccgttgtgtt cgccctttga ttagcaactc tggttttctt tcttagctga acaatctggt
    20161 tatagcgtgc ggactgaccg ttaattttct ccttcttact ggataacaca ccatgagctg
    20221 ctacctttaa aacacctgaa tgtcaacagg ttaactcgtg ccatatcgtt ataatccctt
    20281 tgatattaga tgcaaatta
//
LOCUS       DAETQN010000075        20231 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.075, whole genome shotgun sequence.
ACCESSION   DAETQN010000075 DAETQN010000000
VERSION     DAETQN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.075"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            690..1460
                     /locus_tag="K0W68_002710"
     CDS             690..1460
                     /locus_tag="K0W68_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBI3074233.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1451..1924
                     /locus_tag="K0W68_002711"
     CDS             1451..1924
                     /locus_tag="K0W68_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBI3074234.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2032..2571
                     /gene="dnaT"
                     /locus_tag="K0W68_002712"
     CDS             2032..2571
                     /gene="dnaT"
                     /locus_tag="K0W68_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBI3074235.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2574..3311
                     /gene="dnaC"
                     /locus_tag="K0W68_002713"
     CDS             2574..3311
                     /gene="dnaC"
                     /locus_tag="K0W68_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBI3074236.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3360..3854
                     /locus_tag="K0W68_002714"
     CDS             3360..3854
                     /locus_tag="K0W68_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBI3074237.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4108..6399
                     /gene="opgB"
                     /locus_tag="K0W68_002715"
     CDS             4108..6399
                     /gene="opgB"
                     /locus_tag="K0W68_002715"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBI3074238.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6538..7560)
                     /gene="lgoD"
                     /locus_tag="K0W68_002716"
     CDS             complement(6538..7560)
                     /gene="lgoD"
                     /locus_tag="K0W68_002716"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HBI3074239.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7699..8613
                     /locus_tag="K0W68_002717"
     CDS             7699..8613
                     /locus_tag="K0W68_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI3074240.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8828..10179
                     /locus_tag="K0W68_002718"
                     /pseudo
     CDS             8828..10179
                     /locus_tag="K0W68_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10228..11883)
                     /gene="tsr"
                     /locus_tag="K0W68_002719"
                     /pseudo
     CDS             complement(10228..11883)
                     /gene="tsr"
                     /locus_tag="K0W68_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12002..12448)
                     /gene="hpaR"
                     /locus_tag="K0W68_002720"
     CDS             complement(12002..12448)
                     /gene="hpaR"
                     /locus_tag="K0W68_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBI3074241.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12720..14009
                     /locus_tag="K0W68_002721"
     CDS             12720..14009
                     /locus_tag="K0W68_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HBI3074242.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            14006..15472
                     /gene="hpaE"
                     /locus_tag="K0W68_002722"
     CDS             14006..15472
                     /gene="hpaE"
                     /locus_tag="K0W68_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBI3074243.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15474..16325
                     /gene="hpaD"
                     /locus_tag="K0W68_002723"
     CDS             15474..16325
                     /gene="hpaD"
                     /locus_tag="K0W68_002723"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBI3074244.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16335..16715
                     /locus_tag="K0W68_002724"
     CDS             16335..16715
                     /locus_tag="K0W68_002724"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBI3074245.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16826..17629
                     /gene="hpaH"
                     /locus_tag="K0W68_002725"
     CDS             16826..17629
                     /gene="hpaH"
                     /locus_tag="K0W68_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBI3074246.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17640..18428
                     /gene="hpaI"
                     /locus_tag="K0W68_002726"
     CDS             17640..18428
                     /gene="hpaI"
                     /locus_tag="K0W68_002726"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBI3074247.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18603..19979
                     /gene="hpaX"
                     /locus_tag="K0W68_002727"
     CDS             18603..19979
                     /gene="hpaX"
                     /locus_tag="K0W68_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBI3074248.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19989..>20231
                     /locus_tag="K0W68_002728"
     CDS             19989..>20231
                     /locus_tag="K0W68_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBI3074249.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4870 a         5429 c         5168 g         4764 t
ORIGIN      
        1 tcttttaacc ctgcttgcat aacaggtatt ttactgataa caattccatt tttgcagcat
       61 cctggcaaca tatttcctcc acatccactg tctaaaaatc atgatggttt attcaggata
      121 ttcattcctt aaatgtttcc agcaaattaa tagattaagc gaataaatta tccttagcat
      181 tcactttcat atcagcatac tcctgctgca caacaactcc actgcatgtg tcagaaggtt
      241 agcatcaaat gataacctgg tgaaatagcc tggagacatt taaaatatct acattaagtt
      301 gttaggaata cttaatgaaa aacagaaatc acattcattt aatttataat taaatgatat
      361 cccttcccgc agaatgaaaa atcacacaac caatatttat tcaatgaaat ctgatggttt
      421 taacttgtta acattaaaaa acgtgcccgg ttatctatta tcctgattcg taaaggccaa
      481 acaataaatt cattatagtg ataatattca tgtgatagtt tgtttgcggc gagagataat
      541 tcgcttttta tcaccgagct tgtcccgata accaggatta atcagcgcaa ttttgatctt
      601 aacctaccgc aaaacaaccg tgcttcatga tacaaatgga ttctcattat ctgaacaata
      661 cgcagcacgt ctatgacaaa gggcgagtta tgcaaactga gcaacagcga gccgtaacac
      721 ggctttgtat ccagtgtgga ttatttcttt tgcaacatgg tgcggaaagc gcgttggttg
      781 atgagctttc ctcacgactg ggtcgggcgt tggggatgga cagcgtcgaa agttctatct
      841 cttcgaacgc catagtgctg acaaccatta aagatgggca atgcctgaca tcgacacgta
      901 aaaatcacga tcgcggcatt aatatgcatg tggtgactga agtccagcac attgtgattc
      961 tggcggagca ccatctgctg gattacaaag gcgtagagaa acgatttagc caaattcagc
     1021 cattacgtta cccaagatgg ctggtagcct taatggttgg cctttcttgc gcctgtttct
     1081 gcaaactcaa taaaggtggc tgggatggtg ccgtcatcac cttctttgcc agtacggccg
     1141 cgatgtatat ccgccagctg ctggcacaac gtcatcttca tccgcagatc aacttttgcc
     1201 tcaccgcttt cgccgccacc accatttccg gattgctttt gcaacttccc actttcagca
     1261 atacacccac tatcgcgatg gccgccagcg ttctgctgct ggtgccggga tttccgttga
     1321 ttaatgccgt cgccgatatg tttaaaggcc acatcaatac cggactggca cgctgggcga
     1381 tcgccagtct gctgacactg gctacctgcg tcggcgtggt gatggcactg acgatttggg
     1441 ggctacgcgg atgggtgtga tcgagtttct gtttgcattg gcgcaggata tgatcctcgc
     1501 cgccattcca gcggtcggct ttgcgatggt gttcaacgtt cccgttcggg cattacgctg
     1561 gtgtgcgctg cttggcgcga ttggtcatgg ttcacgaatg atcttaatga ccagcgggct
     1621 gaatattgag tggtcaacct ttatggcctc catgctggtc ggcaccattg gtattcaatg
     1681 gtcgcgctgg tatctggcgc atccgaaagt gtttaccgtg gcggccgtta tccctatgtt
     1741 cccgggcata tcggcttata ccgcaatgat ttcggcggta aaaatcagcc agttaggtta
     1801 cagcgaaccg ttgatgatta ccctgttaac caactttctt acagcttcat cgattgttgg
     1861 tgcgttatcc atcggtcttt ccattcctgg attatggttg taccgcaagc gccctcgcgt
     1921 ataaaattgc ctcgcttaaa gcacacggat gagagacagc ctcctctcct ccgtgtgtta
     1981 ctataaaagt tatctccctt ctcgttcatc gttccatatt tgagaaacag tatgtcttcc
     2041 agagttttga ccccggacgt cgttggtatt gacgccctgg tacacgatca ccaaaccgtt
     2101 ctggcaaaag ctgaaggcgg tgtggttgcc gtatttgcta acaatgcccc ggcgttttat
     2161 gccgtcacgc ctgcacgcct ggctgaactg ctggcgctgg aagaaaagct ggcgcgtccg
     2221 ggaagcgatg tcgctctgga cgatcaactc tatcaggaac cgcaagccgc tcccgttgct
     2281 gtacccatgg ggaaattcgc catgtatccg gactggcaac ccgatgccga ttttatccgc
     2341 ctggcggcgc tatggggtgt ggcactaaga gagccggtga ccaccgaaga actggcctca
     2401 ttcattgcct actggcaggc ggaaggtaaa gtctttcacc atgtgcagtg gcaacaaaaa
     2461 ctggcgcgca gcctgcaaat cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg
     2521 aatacggtca gcgaacctga cagtcaaatt ccaccaggat tcagagggta acgatgaaaa
     2581 acgttggcga cctgatgcaa cgcctgcaaa aaatgatgcc tgcccatatc aaacctgcat
     2641 tcaaaacggg tgaagaacta ctggcctggc agaaagaaca aggagcgatc cgctccgccg
     2701 ctctcgaacg tgaaaatcgg gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc
     2761 ggccactgca tcagaactgc tcctttgaga actatcgcgt tgagtgtgaa gggcagatga
     2821 atgcgttaag caaagcgcgc cagtacgtcg aagagtttga cggcaacatc gccagcttca
     2881 tcttttctgg taagccagga accggcaaaa accatctggc ggcggcaatc tgcaacgagc
     2941 tgctgctacg cggtaaatcc gtattgatca tcaccgtggc cgatattatg tcggcgatga
     3001 aagatacctt caggaatagc ggtaccagtg aagaacaact gcttaacgat ctgagcaacg
     3061 ttgatctgct ggtgatcgat gagatcggcg tgcagaccga atcgaaatac gaaaaagtga
     3121 tcatcaacca gatcgtcgat cgccgctctt cttccaaacg cccaaccggg atgctgacca
     3181 acagtaatat ggaagagatg accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc
     3241 tgggtaacag tttgtgggtg atcttcaact gggatagcta ccgtagccgg gtaacaggta
     3301 aagagtatta atccccgttt ctggcggtat actgcgacac aattccacct tgagtaatga
     3361 tgatgaaaac tgttaaacat cttctgtgct gtgccattgc cgccagcgca ttaatttcca
     3421 ccggggtgca tgctgcgtcc tggaaagatg cgctctccag cgccgccagc gaacttggca
     3481 accaaaacag cacgacacag gaaggcggtt ggtcgctcgc gtcattaact aacttgctta
     3541 gcagcgggaa ccaggcctta agcgcagata acatgaacaa cgccgcaggc attctgcaat
     3601 actgcgcgaa gcaaaagctg gcgtcggtaa ccgatgccga aaacatcaag aaccaggtgc
     3661 tggaaaagct gggcctgaac agtgaagagc aaaaagaaga caccaactat ctggatggca
     3721 ttcagggttt gctgaaaacc aaagatggtc agcaactcaa tctggataac atcggaacga
     3781 ctccgctggc agaaaaggtg aaaaccaaag cctgcgatct ggtgttaaag caggggctga
     3841 acttcatttc ctgaaccgcg aaacgccgcg ttttagctgt tacccggctg acgcggctct
     3901 ctcctgccct tttccctgct cctggacggt tttccccctg ttacacggga agcgaccgcg
     3961 atcaaaaaag tctgtttata aactaattcc taacaacatc gcattttttt gacactagaa
     4021 ttgcagcaat atggcgacgc cattacattg tgcttctcaa aaagcttaaa cgttggctaa
     4081 acggcccggt ttagtgagga tcgcccgttg tcagaactac tctctttcgc cctttttctc
     4141 gcctctgtgc tgatttacgc atggaaagcg ggacgtaaca cctggtggtt tgcagccaca
     4201 ttaacggtgc tggggctatt tgtcgtttta aatatcaccc tgtttgccag cgactatttt
     4261 actggcgatg gtattaacga cgcggttctc tataccttaa ccaacagcct gaccggtgct
     4321 ggcgtcagca aatacatcct gccgggtatc ggcattgtgc tggggctgac agcggtgttc
     4381 ggtgcgctgg gctggatcct gcgccgtcgt cgccatcatc cgcaccattt tggttacagc
     4441 ctgctggcgc tcttactggc gctgggttcg gtggacgcca gcccggcatt tcgtcagata
     4501 acggaactgg tgaaatccca gtcacgcgat ggcgacccgg actttgcggc ttattataaa
     4561 gagccgtcga aaactatccc tgacccgaaa ctcaacctgg tttatatcta cggcgaaagt
     4621 ctcgagcgga cctattttga taacgaagct ttcccggatc tcacgcctga actgggcgcg
     4681 ttgaaaaatg aaggcctgga tttcagccac acgcagcagc tgccaggaac ggattacacg
     4741 attgcgggca tggtggcttc tcagtgcggc ataccgctgt ttgccccctt tgaaggcaac
     4801 gcctccgcct ctgtctccag cttcttcccg cagaacatct gtctgggcga tatcctgaaa
     4861 aactcgggtt atcagaacta tttcgtgcag ggcgcgaatc tgcgttttgc cggtaaagat
     4921 gtgttcctga agtcgcatgg cttcgaccac ttatacggct cagaagagct gaaaagcgtg
     4981 gtggccgacc cgcactatcg caacgactgg ggattctacg acgataccgt tctcgatgaa
     5041 gcgtggaaaa agtttgaaga gctttcccgc tcaggtcagc gattctcact gtttaccctg
     5101 acagtcgata cccatcaccc ggatggtttt atctcgcgta cctgtaaccg caaaaaatat
     5161 gattttgacg gtaagccgaa ccagtcattc agcgcggtaa gttgcagcca ggagaatatc
     5221 gcggcgttta tcaacaaaat caaagcgtca ccgtggttta aagataccgt catcgtcgtc
     5281 tcttctgacc atttagcgat gaacaacacg gcgtggaaat acctcaataa gcaggatcgc
     5341 aataacctgt tttttgtcat tcgtggcgac aagccgcagc aagagacgct ggcagtgaag
     5401 cgtaacacga tggataacgg cgcgacggtg ctggacattc tcggtggcga taactatctc
     5461 ggacttggtc gtagcagttt atccgggcag tcgatgtcgg aaatcttcct caatatcaaa
     5521 gagaaaacat tggcgtggaa gccggatatc atccgcctgt ggaaattccc taaagagatg
     5581 aaagagttca ccatcgacca gcagaaaaac atgattgcct tctcgggtag ccatttccgt
     5641 ttgccgctgc tgttgcgggt ttcagacaaa cgcgtggaac cgctgccgga aagcgaatac
     5701 tcagcaccgc tgcgtttcca gctggccgat ttcgctccac gcgacaattt cgtctgggtt
     5761 gaccgttgct ataagatggc acaactctgg gctccggaac tggcactctc caccgactgg
     5821 tgtgtctcgc aagggcagct tggcggtcag caaattgttc agcatgttga caaaacaaca
     5881 tggcagggca aaacggcatt taaagatacg gtcatcgaca tggcgcgtta taaaggcaat
     5941 gtcgatacgc tgaaaattgt tgataacgat attcgctaca aagccgacag tttcatcttc
     6001 aacgtcgccg gtgcgccaga agaggtgaaa cagtttagtg ggatttcccg tccagagtcg
     6061 tggggccgct ggtccaacgc gcagctgggc gatgaagtaa aaatcgagta caagcatccg
     6121 ctgccaaaga aatttgacct ggtgattacc gccaaagcat acggcaataa cgccagccgt
     6181 cctattccgg tacgcgtagg caatgaagaa caaacccttg tgctgggcaa tgaagtgacc
     6241 accaccacac tgcatttcga taacccaacc gatgccgaca cactggtaat tgtgccgccg
     6301 gaacctgtct caaccaacga agggaatatc ctcggacact cgccgcgtaa gctcgggatc
     6361 ggcatggtgg aaattaaagt ggtggaacgt gaaggataat tcctgactga tgtgtaggtc
     6421 gcataaggtg cgacctacac accttctgcg gcctacaaaa catcattgag aaacagaaaa
     6481 aaacagcgcc caacacttcc ccggacgctg catggcaact ttaataatct cgccttatca
     6541 gaaagtaatt acacccttaa tcaactcacg attgttaatc acatcgcgct cgtaggtttc
     6601 tgccagcgtg gcgaacggat agcgatgggt taacatcatg tcagcagtga tttttccttc
     6661 cgccatcagt cgaccgactt tagcaaaatc ttccggcgtg gcgttgcggc tacccatcat
     6721 cgtcgtttct tttttatgga attccggatc ggagaactgc aactcacctt taaacaggcc
     6781 gacaaatacc accgtgccgc cgtgacgaat caaattcacg gtgttattca tcgcatgttg
     6841 attacctgtc gcgtcgatca ctttctgcgc cagcgaacca ccaaactgcg cccgtagctg
     6901 cgcgtcaaaa tcttcggctg acgggtccag taaaggtaat tccagacgcg ttgccacatg
     6961 ttcacggcgc gccggactgg tatccgccac caccacctgt gcgccatcgg ctttagcgat
     7021 tgccgccgcg cccagaccga ttggccccgc cccgaccacc agcacctgct cgccgggagc
     7081 aatggctgcg cgacgcaccg catgagcgct aatagcgaaa ggttcaatca atgctgccgc
     7141 ctgcgggtca ataccgtctg cgggcaaaat gttcgccacc ggcacgctca aatactcact
     7201 aaaaccgcca tcctgatgca cgccaatgac tgaaattttt tcacagcaat tggtacgccc
     7261 gcttttacac gccgggcatt gctgacaggc aacataaggg atcacagcaa cttgctgacc
     7321 atttttaaga tcagcaatat ttttacccag cccaacaatc tccccacata tttcatggcc
     7381 taaaacacgt ggataactaa aaaatggttg atttccaccc caggcatgaa tatcggtacc
     7441 gcaaatcccg acagacttaa tttttattaa tgcttcattg tcacccggaa taggtatctc
     7501 gcgttgtttc cagactaatt ctttcggctg ctggcaaatt aaaacattca tcgtagacat
     7561 gattcggctc tccatttttt gttgccttag atatcaatga aaatgaaaaa tggcagcgga
     7621 acggaaaatc tgttttgtga aaacacacgc ataaaaatgt tttaaatcgg gttttaatgg
     7681 actcacaagg aggaaaatat gagtcgttca caaaatttac gccacaatgt gattaaccag
     7741 gtcattgatg atatggcgcg gggtcatatc ccttccccac ttccatcgca aagtgcgctg
     7801 gcggaaatgt acaacatcag ccgcaccaca gtgcgtcata ttctcagcca cttacgcgaa
     7861 tgcggcgtct tgacgcaggt tggaaacgac tatgttattg cgcgtaaacc tgaccatgat
     7921 gacggtttcg cctgtactac cgcttcaatg agtgaacaaa acaaagtgtt tgagcaggct
     7981 tttttcacga tgataaacca gcgccagtta cgcccagggg aaacgttttc tgaactgcaa
     8041 ctggctcggg ccgcgggtgt cagcccggtt gtagtaagag aatatctttt aaaattcggc
     8101 cgttataatc ttattcacag cgaaaagcgc ggccagtgga gcatgaagca attcgatcaa
     8161 tcctacgccg agcagctgtt tgagctacgc gaaatgctcg aaacacattc acttcagcac
     8221 tttctcaacc tgcctgatca tgatccccgc tggctgcagg cgaaaaccat gctcgaacgt
     8281 catcggctac tgcgtgacaa catcggcaat agctttcgca tgttctcgca acttgacaga
     8341 gattttcact ccttactgct ttcagctgcc gacaatatct tttttgatca atcgcttgag
     8401 atcatctcag tcatctttca ttttcactat caatgggacg aaagcgatct taaacagcgc
     8461 aatatcattg ccgtcgatga gcatatgacc attctcagcg ctctgatttg ccgcagtgac
     8521 cttgatgcca cccttgcgct ccgtaaccat ttgaattcag ctaaacaatc aatgattcga
     8581 tccattaatg agaacacgcg ttatgctcat taagtacgga ttaaaaacag caaactatta
     8641 gcgggtgcac ggttttatca aaagataaat accgggaaat tcccgcttac ctatgctcac
     8701 aatcagtcga ctaaaaatgt ttttgtgagc ggtagtaaag tcctaaaact ttaacctgcc
     8761 gttaatgtca accttgcgta attcacaatt acgtaaacaa ttaaaattat aaatacagga
     8821 gtccggcgtg gaaaaagaaa atatcaccct cgatccgcgt tcttcattta ctccatcttc
     8881 gtcggcagat attcccgtgc caccagatgg attagttcaa cgcagtaccc gaattaaacg
     8941 cattcaaacc accgccatgt tgttattatt ttttgcggcg gtaatcaatt atctcgaccg
     9001 cagttcgctg tcggtagcaa atttaacgat tcgtgaagaa ttgggattaa gtgccaccga
     9061 aatcggcgct ttgctctccg tgttttcact cgcttacggg attgcgcaac ttccttgcgg
     9121 cccactattg gatcgtaaag gcccacgcct gatgctggga ctggggatgt tcttctggtc
     9181 actgttccag gcaatgtctg gcatggtgca caactttacg cagttcgtgt tggtgcgtat
     9241 cggtatgggg attggtgaag cgccgatgaa cccatgcggt gtaaaagtca ttaacgactg
     9301 gttcaacatc aaagagcgcg gacgcccgat gggcttcttc aacgcagctt ctaccattgg
     9361 cgttgccgta agcccaccga ttctggcggc gatgatgctg gtgatgggct ggcgcgggat
     9421 gtttattacc attggtgtac tggggatttt tctcgccatc ggctggtata tgctctatcg
     9481 caaccgcgag cacgtagaac tgactgccgt tgaacaagct tatctcaatg caggtagcgt
     9541 caatgcccgc cgagatccgc tcagttttgc cgaatggcgc agcctgttcc gtaaccgtac
     9601 aatgtgggga atgatgctcg gattcagtgg catcaactac actgcgtggc tgtatctggc
     9661 ctggcttcct ggttacctgc aaacagccta taacctggat ttaaaaagca cagggttgat
     9721 ggcggctatc cctttcctgt ttggggctgc cgggatgctg gtcaacggtt acgttactga
     9781 ctggctggtc aaagggggaa tggctccgat taaaagccgt aagatctgca ttattgccgg
     9841 gatgttctgt tctgccgcct ttacgctggt cgtaccgcaa gcgacaacat ccatgacagc
     9901 ggttctgctg attggcatgg cactgttctg tattcaacat cctgctgggg cttgatccac
     9961 gtcgcagttg cttctcgcat gactgcgtcg gtgggcagta tccagaactt tgccagcttc
    10021 atctgcgcct cttttgcgcc gatcattact ggttttattg ttgataccac ccactcattc
    10081 cgtctggcac taatcatctg cggttgcgtc accgcagcgg gggcactggc gtacatcttc
    10141 ctggttcgtc agccgatcaa cgacccacgt aaagattaat ctccttatgc ccgataacat
    10201 tttgcttatc gggcattttc atggcgatta aaatgtttcc cagttctcct cgctatctgc
    10261 cacggccatt ttacgcggcg cagctggcgt cacggttttt accacagccg atgtttcacg
    10321 ctgctgttgc tgaatccgga acactgccac tgcttcggtc aggcgactgg cctgctcttc
    10381 cagcgcggcg gcggcagcgg cagactcttc caccagcgcg gcgttctgtt gagttacccg
    10441 gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct catcagaagc
    10501 agaagcaatt tcgcccataa tgtccgtcac gcgggtcacg gcgctgacaa tctccgccat
    10561 tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca ccgagtcttc
    10621 aatcaggctt ttaatttcac gagccgcctg ggcgctgcgc tgggccagat tacgcacttc
    10681 tcccgcgacc accgcaaaac cgcgcccttg ctcacccgca cgcgcagcct caaccgccgc
    10741 gttcaaagcc agaatattgg tctggaaggc aatgccgtca attacgctga taatatcggc
    10801 gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat ctaccacttt
    10861 gccgccgcgt tgcgccgttt cagaagcact taacgccaga tggctggcct ggcgcgcatt
    10921 ttcggcgttc tgcttcaccg ttgcggtcag ttactccatg ctggctgccg tctcttccag
    10981 cgaagcggcc tgttgctcgg tgcgcgaaga gagatcgtta ttgccggtgg cgatttcgct
    11041 ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac gcatcagctc
    11101 tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc catccacctc
    11161 aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca ggcgattcat
    11221 tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg ccagtacgac
    11281 gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt tatcgctgac
    11341 ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct tctcgaaacc
    11401 gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg cacctaacag
    11461 ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga tctctgccgc
    11521 tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc agtttttttc
    11581 cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg aaccgctacc
    11641 aatattgttc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt tacgcgtctg
    11701 caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg tttgtaaaac
    11761 agtgaaattt tctttgtcat tctttaaggc attaaagaac agaccgcctg atgtcagttg
    11821 taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga tacgttttaa
    11881 catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc aaaaacttta
    11941 tgaaaaggtg taacccacca tccatggaaa aaccaggcgc tggttatcag atactaaaaa
    12001 gttattcatt atcgcccggg atggcttctt gtcgggaatt gcccagagca ataaattcct
    12061 ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca atctgccgat
    12121 aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct ttggttagcg
    12181 agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa ccgtcgcgtt
    12241 ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga tacgccagat
    12301 cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac tgttgctcag
    12361 tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct cgcgcctgga
    12421 gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca aaatcattaa
    12481 tatagaaaca gttaatcgtc acgttaccgg gactgcgctg cccctgcaat tcctgatttt
    12541 atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta tctttaaatg
    12601 taaatagttt gttaattaga tcacatttac atcacttact tttgccaaac tacgcaatcg
    12661 ggaataaaag caatcattaa catattaatg attaagatga tcgaatccga ggagtggtaa
    12721 tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat gcatggcagg
    12781 aagcgttcca gcaatccccc tacaaagccc cgcctaaaac tgcggtctgg tttattaaac
    12841 cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt gaaaaggtac
    12901 tgagcggtgc gactgttgcg ctgattgtgg gaaaaacggc gacgaaagta cgtgaagaag
    12961 atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg ccggaagaga
    13021 gcttttaccg cccggcaatc aaagcaaaat gtcgtgatgg attctgcccc attggcgaaa
    13081 ccgtggctct cagcaatgtc gataatctga ccatctatac cgagatcaac gggcgtcctg
    13141 ccgatcactg gaataccgcc gatttacaac gtaacgccgc gcagttgctg agcgccctga
    13201 gcgaatttgc cacgctgaat ccaggcgacg ccattctgct cggcacgcca caggcgcgcg
    13261 tggaaataca gccaggcgat cgcgttcgtg ttctcgcaga aggtttcccg ccgctggaaa
    13321 atccggtagt ggacgaacgt gaagtgacca cgcacaagag cttcccaacg cagccacacc
    13381 cgcacggcac gctgtttgcc ctcggcctga actacgccga ccacgccagc gaactggaat
    13441 ttaagccacc ggaagaaccg ctggtgttcc tgaaagcgcc aaataccctc actggcgata
    13501 accagacctc cgtgcgtcca aacaatattg aatacatgca ctatgaagcg gagctggtgg
    13561 tagttattgg caaacaggcg cgtaacgtca gcgaagccga tgccatggat tatgtcgcgg
    13621 gctacaccgt gtgtaacgac tacgccattc gtgactatct ggaaaactac taccgcccta
    13681 acctgcgggt aaaaagccgc gacggactga cgccgatgct ttcaaccatc gtgccgaaag
    13741 aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc gagttacgcc
    13801 agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc tacttaagtg
    13861 aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacacccaaa ggcttatctg
    13921 acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc ctggtgaacc
    13981 gaattgtgag tgaggataca gcgaaatgaa aaaagtaaat cattggatca acggcaaaaa
    14041 tgttgcaggt aacgactact tccagaccac caatccggca acgggtgaag tgctggcgga
    14101 tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga aagaggcgtt
    14161 cccgaaatgg gccaatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc gtctgggcga
    14221 tctgatcgac cagaacgtgc cagagatcgc cgcgatggaa accgcggaca ccggcctgcc
    14281 gatccatcag accaaaaatg tgttgatccc acgcgcttcc cacaactttg aatttttcgc
    14341 ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga tgctcaacta
    14401 cacgctggtg cagccggtgg gcgtttgtgc gctggtatcg ccgtggaacg taccgtttat
    14461 gaccgccaca tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg tactgaaaat
    14521 gtcggaactc tccccgctga ccgctgacag cctgggtgag ctggcgctgg aagccgatat
    14581 tccggcaggc gtgctgaacg tggtacaggg ctacggcgca accgcagggg atgcgctggt
    14641 tcgtcatcat gacgtacgtg ccgtgtcgtt caccggcggt acggccaccg ggcgcaacat
    14701 catgaaaaac gccgggctga aaaaatactc catggaactg ggcggtaaat cgccggtgct
    14761 gatttttgaa gatgccgata ttgaacgcgc gctggacgcc gccctgttca ccatcttctc
    14821 gatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcagcaaa gcatctaccc
    14881 ggaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg atccgaacga
    14941 tccgaatacc caggttggcg cgcttattag ccagcagcac tgggaaaaag tctccggcta
    15001 tatccgtctc ggcattgaag aaggcgcaac cctgctggcg ggcggcccgg ataaaccgtc
    15061 cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaaccgtgc tggcagacgt
    15121 tgataaccgt atgcgagtcg cccaggaaga gattttcggg ccggtcgcct gcctgctgcc
    15181 gtttaaagac gaagccgaag gcttacgtct ggcaaacgac gtggagtacg gcctcgcgtc
    15241 gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca ttgaagctgg
    15301 catggtgttc gtcaacaccc agaacgtgcg tgacctgcgc cagccatttg gcggcgtaaa
    15361 agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg cggaaatgaa
    15421 gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct gatatgggta
    15481 agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatcactct gaactgccag
    15541 ggaaaaacca cggttgccgc cagggcgcga tcgacgggca taaagagatc agcaagcgtt
    15601 gccgggaaat gggcgtcgat accattatcg ttttcgatac ccactggctg gtcaacagtg
    15661 cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac gagctgccgc
    15721 attttatccg cgatatgacc tacaactacg agggcaaccc ggagttgggg cagcttattg
    15781 ccgatgaagc cttaaagctc ggcgtgcggg caaaagcgca caacattccc agcctgaaac
    15841 tggaatacgg cacgctggtg ccgatgcgct acatgaatga agacaagcac ttcaaagtgg
    15901 tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag ctgggcgaag
    15961 cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc agcggttcgt
    16021 tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc tacacccgcg
    16081 agttcgaccg ccagatggac gagcgcgtgg tgaagttgtg gcgcgaaggc cagttcaaag
    16141 agttctgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc aatatgcacg
    16201 acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag gtggagttta
    16261 tcaccgagct attcccaagc tctggcaccg gtcaggttaa cgctgttttc ccgcttcccg
    16321 cgtaaggacg ttttatgccg cactttatcg ttaaatgcag tgacaacatc cgcgaagaag
    16381 ccgacctgcc ggggttgttc gccaaagtga atccgacgct ggcagccacg ggtatttttc
    16441 cgctggcggg tattcgcagc cgcgtgcatt gggtcgatac ctggcagatg gccgacgggc
    16501 agcgtgatta tgccttcgtg catatgacgt tgaaaatcgg cgcaggtcgc agcctggaaa
    16561 gccgccagca ggcgggtgaa atgctgtttg aactgattaa aacgcacttc gccgccctga
    16621 tggagagccg cctgctggcg ttgtcgtttg agattgaaga gctgcatccg acgctgaatt
    16681 ttaaacaaaa caacgtgcac gcattgttta agtagcgcgc agattgcccg gtggcgctgc
    16741 gcttaccggg cctacaaaac cccaaaccgt acaccgtagg ccggataagg cgcagccgca
    16801 tccggcaatg ccacaggata tcgctatgtt cgataaacac acccacaccc tgatcgccca
    16861 gcgtctggat caggcagaaa aacagcgcga acagatccgc gcgatctcgc tggattaccc
    16921 ggagatcacc atcgaagacg cttacgcggt gcagcgtgaa tgggttcgcc tgaaaatcgc
    16981 cgaaggtcgc acgctgaaag gccacaaaat cggcctgacc tcgaaagcga tgcaggcaag
    17041 ctcgcagatc agcgaaccgg attacggcgc gctgctggat gacatgttct tccacgatgg
    17101 cagcgatatc ccgaccgatc gctttatcgt gccgcgcatt gaagtggagc tggctttcgt
    17161 gctggcaaaa ccgctgcgtg ggccaaactg cacgctgttc gacgtctaca acgccacgga
    17221 ctacgtgatc ccggcgctgg agctgatcga cgctcgctgc cacaacatcg atccggaaac
    17281 ccagcgcccg cgcaaagtgt tcgacaccat ttctgataac gccgccaacg ccggggtgat
    17341 cctcggcggt cgtccgatca aaccagacga actggatctg cgttggatct ccgccctgat
    17401 gtatcgcaat ggcgtgattg aagaaaccgg cgtcgccgct ggcgtgctga atcatccggc
    17461 aaacggcgtg gcctggctgg cgaacaaact cgcaccgtat gacgtacaac tggaagccgg
    17521 gcaaatcatt ctcggtggtt cgttcacccg cccggttccg gcgcgtaagg gcgacacctt
    17581 ccacgtcgat tacggcaaca tgggttccat tagctgccgc tttgtttaag gagagaacga
    17641 tggaaaacag ctttaaagcg gcgctgaaag caggccgtcc gcagatcgga ttatggctgg
    17701 ggctgagtag cagctacagc gcagagttac tggccggagc aggattcgac tggttgttga
    17761 tcgacggtga gcacacgccg aataacgtgc aaaccgtgct cacccagcta caggcgattg
    17821 cgccctaccc cagccagccg gtagtacgtc cgtcgtggaa cgatccggtg caaatcaaac
    17881 aactgctgga cgtcggcaca caaaccttgc tggtgccgat ggtacaaaac gctgacgaag
    17941 cccgtgaagc ggtacgcgcc acccgttatc cccccgccgg tattcgcggt gtgggcagtg
    18001 cgctggcccg cgcctcgcgc tggaatcgta ttcctgatta cctgcaaaaa gccaacgatc
    18061 aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat gaagaactta ccgcagattc
    18121 tggacgtgga aggcgtcgac ggcgtgttta tcggcccggc ggatctgagc gccgatatgg
    18181 gttatgccgg taatccgcag cacccggaag tacaggccgc cattgagcag gcgatcgtgc
    18241 agatccgcga agcgggcaaa gcgccgggga tcctgatcgc caatgagcaa ctggcaaaac
    18301 gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt tgacaccacc ctgctcgccc
    18361 gcgccgccga agcgctggca gcacggtttg gtgcgcaggc caccgccgtg aagcccggcg
    18421 tgtattaatg ccggatgcgc tgcgcttatc cggcctacac tcgcaccaaa ccgtaggccg
    18481 gataaggcgt ttacgccgca tccggcaaaa agccgtacca aatcgcggac cggataaggc
    18541 gttacgccgc atccgccata aaccttgtcg taccctacaa aaatcccatt agaggaagaa
    18601 aaatgagcga cacctcacct gccataccgg agagtatcga tccggcgaat cagcataaag
    18661 cgctgactgc cggacaacag gcggttatta agaagctatt tcgccgcctg agcgtctttc
    18721 tgttcgtgct gtttatcttc tcgttccttg atcgcatcaa catcggcttt gccggactca
    18781 cgatgggacg cgacctcggt ctgagcgcca ccatgtttgg cctcgctacc accctgttct
    18841 atgccgctta tgtcatcttc ggcattccca gcaacattat gctgagtatt gtcggtgcgc
    18901 ggcgctggat cgccaccatc atggtgctct ggggcatcgc ctctactgcc accatgtttg
    18961 ccactggccc caccagcttg tacgtactgc gtatactggt tggcattacc gaagccggct
    19021 ttctgcctgg tattctgctg tatttaacct tctggtttcc ggcctatttc cgcgcccgtg
    19081 ccaacgcctt gtttatggta gcaatgccgg taacgacagc gttgggctcg atcgtttccg
    19141 gctacatttt gtcgctggat ggcgtcatgg cattaaaagg ctggcagtgg ctgtttttgc
    19201 tggaaggctt cccgtcggta ttactcggcg tcatggtgtg gttctggctt gatgactcac
    19261 cggacaaagc taagtggctg acgaaagaag acaaaaaatg cctgcaagag atgatggata
    19321 acgatcgtct gacgctggtt cagccagagg gagccatcag ccaccacgcc atgcaacaac
    19381 gcagtatgtg gcgggagatc ttcactccgg tggtgatgat gtataccctg gcgtatttct
    19441 gcctgaccaa cacacttagt gcgatcagca tctggacacc gcagatcctg caaagtttta
    19501 atcagggcag cagtaatatc accatcggca tgctggccgc cgtaccgcag atttgtacca
    19561 ttctcgggat ggtctactgg agccgtcact cagatcgccg ccaggaacga aggcatcaca
    19621 ccgcccttcc ttatttgttc gctgccgcag gttggttact ggcttcggca actgatcaca
    19681 acatgatcca gatgctgggg atcattatgg cttcgaccgg atcattcagc gcaatggcga
    19741 ttttctggac aacaccggat cagtccatca gcctgcgggc acgagcgatc ggtattgcgg
    19801 tgatcaacgc cactggcaac attggttcag cgttaagtcc gtttatgatc ggctggttga
    19861 aagatttgac cggcagcttt aacagtggat tgtggtttgt tgccgcgctg ctggtgattg
    19921 gtgcggggat tatctgggcg attccaatgc agtcctcccg tccgcgagcg accctgtaag
    19981 gaacgacgat gtgtgaccgt cagattgcca atattgatat cagcaaagag tacgatgaaa
    20041 gcctgggcac ggacgatgtg cattatcagt cgttcgcccg catggcggca ttttttggcc
    20101 gccatatgct gccacatcgc cacgaacagt actttcagat gcatttcctc aatagcggac
    20161 agattgagct acagcttgac gatcatcgct actcggtgga agcgcccctg tttgtcctga
    20221 cgccgccgtc a
//
LOCUS       DAETQN010000076        20147 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.076, whole genome shotgun sequence.
ACCESSION   DAETQN010000076 DAETQN010000000
VERSION     DAETQN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.076"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="K0W68_002729"
     CDS             complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="K0W68_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HBI3074250.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQ"
     gene            complement(2109..2687)
                     /gene="mocA"
                     /locus_tag="K0W68_002730"
     CDS             complement(2109..2687)
                     /gene="mocA"
                     /locus_tag="K0W68_002730"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBI3074251.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            2790..3560
                     /gene="yqeC"
                     /locus_tag="K0W68_002731"
     CDS             2790..3560
                     /gene="yqeC"
                     /locus_tag="K0W68_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBI3074252.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            3608..5233
                     /locus_tag="K0W68_002732"
     CDS             3608..5233
                     /locus_tag="K0W68_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBI3074253.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(5274..6206)
                     /gene="arcC"
                     /locus_tag="K0W68_002733"
     CDS             complement(5274..6206)
                     /gene="arcC"
                     /locus_tag="K0W68_002733"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBI3074254.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFVRSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(6254..7638)
                     /gene="hyuA"
                     /locus_tag="K0W68_002734"
                     /pseudo
     CDS             complement(6254..7638)
                     /gene="hyuA"
                     /locus_tag="K0W68_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            complement(7691..8902)
                     /locus_tag="K0W68_002735"
     CDS             complement(7691..8902)
                     /locus_tag="K0W68_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBI3074255.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(8960..10156)
                     /gene="dpaL"
                     /locus_tag="K0W68_002736"
     CDS             complement(8960..10156)
                     /gene="dpaL"
                     /locus_tag="K0W68_002736"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBI3074256.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(10214..11401)
                     /gene="ygeW"
                     /locus_tag="K0W68_002737"
     CDS             complement(10214..11401)
                     /gene="ygeW"
                     /locus_tag="K0W68_002737"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HBI3074257.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            11880..13658
                     /locus_tag="K0W68_002738"
     CDS             11880..13658
                     /locus_tag="K0W68_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HBI3074258.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(13698..14177)
                     /gene="xdhC"
                     /locus_tag="K0W68_002739"
     CDS             complement(13698..14177)
                     /gene="xdhC"
                     /locus_tag="K0W68_002739"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBI3074259.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(14174..15052)
                     /gene="xdhB"
                     /locus_tag="K0W68_002740"
     CDS             complement(14174..15052)
                     /gene="xdhB"
                     /locus_tag="K0W68_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBI3074260.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(15063..17360)
                     /gene="xdhA"
                     /locus_tag="K0W68_002741"
     CDS             complement(15063..17360)
                     /gene="xdhA"
                     /locus_tag="K0W68_002741"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBI3074261.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            17775..18530
                     /locus_tag="K0W68_002742"
     CDS             17775..18530
                     /locus_tag="K0W68_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBI3074262.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            18609..18678
                     /locus_tag="K0W68_002743"
     tRNA            18609..18678
                     /locus_tag="K0W68_002743"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18641..18643,aa:Gly,seq:ccc)
     gene            18871..>20147
                     /locus_tag="K0W68_002744"
     CDS             18871..>20147
                     /locus_tag="K0W68_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074263.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STLDQIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT         4980 a         4765 c         5179 g         5223 t
ORIGIN      
        1 aactgaggaa tgatattttt caccacgccg ccagcattgg cttcgcgttc aaacagcgta
       61 accggatggc ccgctctggc aaggaagtaa cctgctgcca gacccgccgg acctgcacca
      121 atcacggcaa ccggatggcg tgaaccagaa ccggcgggtt tgtgccagcg ttgcttatat
      181 tcatcccaac ctttttccag cgcgactttt ttcagttcgc ggatattcag cgcactgtcg
      241 taatccaggc gggtacagtt gtattggcac tggtgatcgc aaatatgacc ggtaatggca
      301 ggtagggcgt tgcgttggta gatgagttcc agcgcgtcgg catagcggtg ttcgccaagc
      361 agacggatgt attccggaat atcttgcttg atagcgcagg cagtaacaca gggggcaacg
      421 tagcaatcgg tcagcggcag gtcttctgcc acttcaatac gctcttctgg cttccagtgt
      481 ttctgggtgt attccatggt taacgcgtct gctgccagtc tgttcagtcg ttcgacgtca
      541 acatggtcaa gtccccaggc gtcggagcct tccagctcgc gcatgcaggc acttaagcgc
      601 agatagccgc caggtttcag caggtcggtt gccatagtaa tagggcgaat acctgtatca
      661 aaaatatcgc ggatagtcag ctgactggca ccaccggaat aagaaatggg cagtttgccg
      721 tcaaaggcgc gagagagaac tgctgcaaca ttgatggaga gcgggaacag cgcacggcct
      781 gacatataca tctcttcacc aggcagtgcg cctttattgt tgatggtgcc gagagtgtta
      841 gtcagtttta cgccaaagcc gagtgatttt tcttttgcca gtaccatcag gcgctccagc
      901 atttccagtg cttgcgtcag cttgaggtcg tgatcaaatg actcttcttt taagcctatg
      961 taaccgaaac cgcagacatc gaggatctca cgaacacgcg cgtaccccag taaggtcggg
     1021 ttaagtttga caaaggtgtt gagccctttt tcttccagca tgtagcggca aatggcttcg
     1081 atttcatgcg gaggacagcc gtgcatggtg gagaggggga cgccctgcac catactggtg
     1141 gggatgcgag cgggtaaggc ttgcaagttt tcgcgttttt cctgcaatcc gtggcgggct
     1201 aaaaatgcgt catcctggag taatttattc agcgtatcgc gatattgagc gaatttcgga
     1261 tggtcagatg cgtccatcat attgtcgatg aactgttgca tcggcggttg cttaatacct
     1321 tcgaggttgt aaccgatgct catattaaag atgaacgatt taccggaatc agaaggctgt
     1381 aacatcgctt cgagaaggtg cagggcaaac caggctttga ggtattcatc ccaggcttta
     1441 agcaaggtaa attcggtaga ccattcggtg ttaaagcatt cgtcttcggc atcaatacag
     1501 ggcttttcca gctccaggcg gtcaagaatt tggacggttt ttagttcgat gaagcgtccg
     1561 ccagtcagcc aggaagtgac aatattttgc gcgagctgcg tgtgcgggcc agcggcaggg
     1621 ccgacgggag tggcacaggt ttcaccgaag acgctaacag ttttaccttt tacaggtgag
     1681 taaaactgtt gctcgggaat accaaagatt gagcgttgtt gttggtattc atcaaatatg
     1741 cgcgtcaaaa gttcctcaaa cggaatggga cgcataatat cccccataac cctctcctta
     1801 gcttatacag atatcattga gtgaccggtg ttgagaaatc cggtggttta gggggataga
     1861 gcaacaatcg caccactctt gccaggtttg gcgggaaata taaaaaaatg tgaggtggct
     1921 cgaaacttaa aggctttttg tgaatgaact cacaggtgcc aaatgagaat taaaatcact
     1981 cctgaaaatt attattgtca gcccatttat cactttttct cttttctcaa aatcagtgat
     2041 tccttatcat tatgataaag agtgctggag agattaaata tttgaacgca atcgtttaac
     2101 tacattttct aaattttagt ataccttttt tgcgctgtaa taaagtcgtc cggagtatca
     2161 atatctaaaa ttatttctgc attttctatt tcgacggaat aatgatctcc cataaggagt
     2221 gcctgacgca tattggtgac attgggtcgc tggattgcct gcatcaggca tggttttgac
     2281 actaaaatcg gatggccggg gatgccatta tggagtggca gtattgcgcc atcatttcgt
     2341 aacgaccaga tttttctaaa aatatcgatg gtgagggttg gcatgtcacc gtgggtgaga
     2401 aaacaatgtt ctgtttgcac tgcgggtact gcggccttga cggatgttag taaaccctgc
     2461 gcataatctg ggttgtgaat aatagtgata ttgctctggt tcgcataacg ttcgtgcagt
     2521 tcattaccac gatagccggt gactaaaata attcggctac aaaactgcaa cgcatttttg
     2581 atacttgtat caagaattgt tccctgttcc caggggaaca tcattttcca ttgccccatt
     2641 cttgatgata atccggcagc ggtaattata cagtcgatgg ctgacatagt ggttccatta
     2701 tttatagggg ctaataacgc ctgataattg cgttacttcc tgatggatta agttggggat
     2761 aatttatccc agagaggtca taaagactcg tgaaaagtat agttgaccca tcggcattag
     2821 tcattgattt aggtgcgcag aaacgtccca ctgtgatttc tgttgttggt gctgggggga
     2881 aaaccagctt gcttttttgg ctggcagaac tgctccaggc tagcggcagg cgtgtgttaa
     2941 tcacgacgac gacacatatg tttatgccaa cgtctcactg gcccgtggtt ttctgtcgtg
     3001 atcccgccat gcttcctcat gcgtctctta catcccccat ttcattttgt tttcacagct
     3061 ggaaagcgaa ccagggaaaa gtgcagggat ttacgccaga agcgattgat gcactggtgc
     3121 aacgaccaga gtgtgacgta attctcattg aagcagatgg ctcgcgtgga atgccgttaa
     3181 aagcgcctga tgagcacgaa ccttgcatac ccaaaagcag ttgctgcgtg attgctgtga
     3241 tgggaggaca tactttgggc gcgaaagtga gcacagaaaa tgtccatcgc tggtcgcagt
     3301 ttgctgatat taccgggtta acacctgatg caaccttgca actgagcgat ctcgttgcgc
     3361 tggttcgcca ccctcagggg gcgtttaaaa acgtaccgca aggttgtcgg cgagtctggt
     3421 tcattaaccg tttttctcaa tgtgagaatg cgattgcaca aagcgagctc cttcaaccgc
     3481 tgcaacaaca cgacgtagag gcaatctggc tgggcgatat acaagaacat cctgcaatcg
     3541 cgcgcagatt tgtgaattag cggcccgggt attccgggtt actgaaagga tatccattga
     3601 ggttggtatg aatattttca cagaggctgc aaaactcgaa gagcaaaatt gtccgtttgc
     3661 gatggcgcaa attgttgata gccgaggctc gactccccgc cattctgcac aaatgttagt
     3721 gcgcgccgat ggttctatcg tcggtacaat tggtggcgga atggttgagc ggaaggtgat
     3781 tgaagagtca cttcaggcat tgcaggaacg taagccgcga ttattccatg gacgtatggc
     3841 tcgtaacggt gcggatgctg tcgggtcaga ttgtggaggt gcaatgtcag tatttatcag
     3901 cgtccatggt atgcgtccac gtctggtgtt gattggcgcg gggcatgtca accgggcgat
     3961 agcccagagt gcggcgctat taggatttga tattgccgtt gccgatattt atcgcgaaag
     4021 cctcaatcct gaactattcc caccatcaac cacgcttctc catgctgagt cgtttggtgc
     4081 ggcagtggaa gaactggata ttcgccctga taattttgtc ctgattgcca cgaataatca
     4141 ggatcgtgaa gccctcgaca aactcattga acagcccatt gcatggttgg ggttgctggc
     4201 aagtcgccgc aaggttcagc ttttcctgcg tcaattgcgt gagaaaggcg tggctgaaga
     4261 acatattgcc cgtttacatg cgcccgttgg ttacaacata ggtgcggaaa cgccgcagga
     4321 gatcgccatc agcgtgctgg cagaaatatt atgggtgaaa aataacgcgc cgggtgggct
     4381 gatgatgaaa ccttctcatc cttccggaca ccagctggtg gtgattcgcg gtgcgggaga
     4441 tatcgccagt ggtgtggcgc tacgtctgta tcatgcgggt tttaaagtga tcatgctgga
     4501 agtggaaaaa ccgacagtga ttcgttgtac cgtggcgttt gcccaggccg tgttcgatgg
     4561 cgaaatgacg gtcgaaggcg tcactgctcg cctggcaacc agctctgcgg aagcgatgaa
     4621 acttaccgaa cgcggattca tccctgtgat ggtagatccc gcctgttcat tgcttgatga
     4681 actgaaaccg ctttgcgtgg tggacgctat tctggcgaaa cagaatttgg gaacgcgggc
     4741 agatatggca ccagtaacaa tcgcgcttgg gccgggcttt actgcaggga aggattgtca
     4801 tgcggtaatt gaaacaaatc gcgggcactg gctcggtcag gtgatttact ctggttgtgc
     4861 gcaggagaat accgatgttc ctggcaatat tatggggcat accacccgac gggtgatccg
     4921 tgctcctgct gcaggcatta tgcgatccaa cgtgaaatta ggcgatctgg tgaaagaggg
     4981 cgatgtgatt gcctggattg gtgagcatga aattaaagca ccgttgacgg ggatggtgcg
     5041 tggcttgttg aacgacggcc tggcagtggt tggtggtttt aaaattggtg atatcgatcc
     5101 tcgtggtgaa accgctgatt tcaccagcgt ttctgataaa gcccgggcga ttggcggcgg
     5161 cgtacttgag gcgttaatga tgttgatgca tcagggcgtg aaagcgacaa aagaagtgct
     5221 ggaagtggct taaatgaaaa tggccggatg gtattaccca cccggcctta aaattactga
     5281 caaatcaccg ttccggtttt gccttcaatc ccttctttcg ctttgctcaa tacggtaatc
     5341 agtgcttcac gacctgcgcg agagcgaaca aacgaggcgg cagcttccac tttaggcagc
     5401 atagaacctt tcgcaaaatg accttcttcg ataaagcgtt cagcatcact taatgacagg
     5461 cgatcgagcc actgttcatt ctctttaccg aagttaatgg ccactttttc taccgcagtc
     5521 agaatgatca gcatatcggc atcaatcatt tctgctaaac gggcgctggc ccagtcttta
     5581 tcgataaccg cgctggcacc acgcagatgg ttaccttcac gaataacagg gataccacca
     5641 ccgccaacgg cgatcaccac ctggcctgca tctaccagag ctttaaccgt ttctttttca
     5701 atgatatcaa ccggttttgg cgaggcaaca acacggcgat agccgcgacc agcatcttct
     5761 ttcagagtgt aaccctgttt tgtcagttgc tctgcttctt gctcggtaaa gaacgaaccg
     5821 atcggcttgg tcgggttgag gaatgccgga tcgttagcgt cgacttcgac ctgagtaacc
     5881 agcgttgcta caggtttatt aatgccccga gaaagcagtt cttctcttaa cgcgttttgt
     5941 agatcgtaac caatataacc ctggcttaag gcaacacaga cagacatcgg cagcattggc
     6001 gagtgtgctt cagtttttgc ggcagcttca aaggcttggt taatcatgcc cacctgcggc
     6061 ccgttaccat gagtgacgat aacttcatgt ccttgggcaa ttaaatcaac aattgcctga
     6121 gaagtaattt ttaccgcttt catttgtccg gccaggtcat cgcccagcgc attcccgccc
     6181 agggcgagaa caattttctt actcattttt cctcactcat cattcttgat tgtgtataga
     6241 tccctgacag tggttagagc acgggaggga caaacggttt gcgtcgcagg aaacgacctc
     6301 gcccggcttt gcctgtaaag gtgccgtcac agaaaatcgt ttcaccacgg gataaggttc
     6361 tgacaatcgc gccctgacag gtaaaaccct cccatggcga gtagtcggcg ttgtcgtgga
     6421 gatggcgatg ctgaatttgt tggctctgac gtgggtcgat aatcaccacg tcgccatcgg
     6481 aaccgggcgc taataatcct ttttgcggcc acaggccaaa cagcctggcg ggcattgcgc
     6541 tggttaattc aacaaagcgt tccggtgtta tacgtcccgt catcacgcca ctggaaaaca
     6601 gtaactgcat gcggttttcc acaccgggta agccatttgg gcagcgactg aaatcgcctt
     6661 tagaaatttg caggcgttga gccatcgaga aggtgcagtg atcggtcgcc accacgtcaa
     6721 tcgcaccatc gctgatgcca caccacagtt tgtcctgctc gcgtacgtta cgcagcggtg
     6781 ggctaagaat gaacttcatg ccatcttctg tatcgtaact gcgttcgtcc aacaggagat
     6841 attgtgggca ggtttcaacc cagactggct ggtgattcgc acgggcaaga cgcagataat
     6901 ccagacctaa gccgttagac aggtgcacga tatagagcgg ggcgttaccg gcaatttgtg
     6961 ccaggttaat catgcgggcg atggcttccg cttcgcattc cagagggcga ctcaaggcat
     7021 gatagcgcgg cgcggttaac cctgcggcga taaattccgc ccgcttgctg gcgatagccg
     7081 catcattttc cgggtgcacg gtggtcagcg cgccggattc atgcaagcgg cgtaatgcct
     7141 gcaaaacctc gtcatcgttg agtttgtatt gataggttaa atagagttta aaactgctca
     7201 gtccttcctc gaccatcatc gggatttcgt cgaggattgc gtgattaatg tgctggatca
     7261 caccgtgaaa gctgtaatcg atgaccgctt tatgggcggc ataaccacga taaacctcca
     7321 gttgatggcg taaccgacag ccgtttgggc caaatcccat atggtcaata atggttgttg
     7381 taccgccaca cgcagctgcg cgggtaccgg taaaaaatca tcacaactgc gcgcgatgcc
     7441 gacatcaata ttgaaatgcg tatggacatc cacgccgcca gggaaaacgt aacagccagt
     7501 ggcatcaatt tcttcatacg ggagctgcgg cgaaatattg ttgcccaact ggcgaacaat
     7561 cccgctttca atcagcaaat cctgtttggc ttgtccatct gcgttaacga cagtgccgtt
     7621 tttgatcaat acgcgcatag caaactccaa aaaccctccg gcgaaccgga ggggaataag
     7681 tgaagagtaa ttactctgtt gccagccagc ttaacgggat tgcagcgtac atcgcggcac
     7741 aggtcaccag gtgagatttc caggtttttt cgttaggtgc atgcgcttca ggttctttac
     7801 ccgggccaaa gccgatcacc ggaatgccgt gacggcccat gatagatacg ccgttagttg
     7861 agaaggtcca cttatcaaca accggcgctt tgccaaacag cccttcgtag gcattcacca
     7921 gtgctttaac ggtgaagtga tcttcttcca ctttccaggt cgggaagtag cattcggttg
     7981 ggtaaaccag gccagtccag gacggacggt cgtagttgta catagaaaca accgcgttag
     8041 ctttctgtac tgcaggcagg gcgcggattt cgtccagcgc gccttcccag gtttcgcccc
     8101 aggtcagacg gcggtcaata gagacggcgc agctatctgc tacagcgcaa cggcttgggg
     8161 atgtgaagaa gatttcagaa acggtgaggg tgcctttgcc gaggaattcg tcataaccca
     8221 gacgttggga gagttcttgt aattcgccaa gaatcggacc cattttgaaa atggcgttgt
     8281 caccgcgttc tggcgcagaa ccgtggcagc taacaccctg aacatcaata cgaatttcca
     8341 tacgaccgcg ctgaccacgg tatatctggc agtcggttgg ttcggtactg accacaaatt
     8401 ccgggcgaat gccggattgt tcaataatgt actgccagca cagaccgtcg cagtcttctt
     8461 cctgcacagt accggtaacc agcagggtat attcatcttc cagaccgagg tctttaatga
     8521 ttttaccggc ataaaccata gatgccatgc cgccttcctg gtcggaagta ccgcgaccac
     8581 cgattagctc atcagtttcc atgccttcgt acggatcgaa gtcccagttt ttgatgttgc
     8641 caatgccgac ggtatcgata tgagcgtcca ttgccaccag acgcgggccg tggccgatat
     8701 aaccgagaac gttgcccatc gggtcgattt caactttatc gaagccgact ttttccatct
     8761 cttctttaat acgatgtact acgcgttttt cgtcgcagct ttcactggga atagcaacca
     8821 tgtcgcgcag gaagcgagtc atgtccgcct ggtaatcttt tgctttttca agaatcagtt
     8881 tgaatggaat attcttagcc attatttttc tccagatttt ttactccccg ttttgcaggg
     8941 agtagttcca atagggtgat taaggtgcta cagcgtgttt gccttcccag acaacttcgc
     9001 ggtagtgctt cacgtcggtg tcgccttcag tgctgataac cagcactacg gcatctttgt
     9061 ccagcgccag tttttccatc aggctttgat gttgcgggtg ataatgaacc gctgcgagaa
     9121 cgcccaaacc gacagcgccg gattcaccgg agatgatgcg cgggtcgttg ccgtacggat
     9181 tacccagcac gcgcatacct aatgcggcaa cgctgtcctg gcaggagatg aattgggtgg
     9241 cgcagttacg taggatttcc cagcccagcg ggttaggttc gccacaggcc aggcccgcca
     9301 tgatggtggc catatcaccg ccaacgttga cgatgtcgcc tttgacgccg gagcgataaa
     9361 tacagtcagc tttgtcaggt tcaacaataa tgctgtgcag attttgcggg ctgtagacgt
     9421 cgaccagata acccagcaca ccaccggcca ttgctccgac accggcttgc agcagaacgt
     9481 gcgtcggggt tacgcccatt tcacgcattt gctcgacggc ttcatctgcc agggttgcgt
     9541 agccttgcat gatccaggtt gggattttgg tgtaaccttc ccatgccgtg tcctgtacca
     9601 cttcccagcc gtgctgctgc gcgtgttgca tggtcaggcg aacggtatcg tcatagttca
     9661 tatccgtgac gatgcactcg gcaccgaggt tcagaatggc gtcaacgcgt tcctgagcag
     9721 aacctttcgg catgtaaatc accgcattct gtccgagttg ctgtgctgcc cacgccacac
     9781 cgcgcccgtg gttgccgtcg gtggtggtcg cgaaagtcat tttttcgccg atggcatttt
     9841 tcaggtgctc aaatgacagc gtttcgatat caagatgata tttttcacac aataattgag
     9901 cgatggcgta cgcaccgcca agcattttga aggcgttcag accgaatcgt tttgattcgt
     9961 ctttgacgag aattttcttc acaccaaaaa ggtttgcgag atcgtccagc gcacaaagcg
    10021 gtgttgggcg ataaccagct attttctggt ggaactggcg cgccagtttg gcctggcttt
    10081 gcgaaaagag cggtgatgtt tcgccgttga aaaatttgtt atcggcgata tcaatcttca
    10141 atgagaaaac ggacatatcc tatccttttg gcctctggaa atagagggcc gaccagcaga
    10201 tcggcccgag gggttatttc acgcgttctt gcgcttcttt cagcagttgc tcgagcagtg
    10261 cacctggttt ggcgtattta cgggacagga tcatcgcggc gatgatataa ggcttccagc
    10321 tggcttcttt gtaggtagcg atacggtatt tttcgaatac gccttcagtc acttcacctt
    10381 ctttgcagga tacgccgctg atatcagctg gcaggcagtg catgtacagg gcttcgccat
    10441 cacgggtcag ttccatcatc tcttcagtac aatgccagtc tttgtgttgc gcgttctgtg
    10501 ccagacactg tttttccagt gcttttaagc cttcgtggtc gttcgcacgc agcaattcag
    10561 tacgctcttc catcactttg taaggtgccc atgacttcgg ataaacgatg tctgcgtctt
    10621 tgaaggcttc ttccatgctg gtgacctgac ggaagctacc accggaggct ttagcattgt
    10681 ttttcgccac ttcaaccaca tccgggatca ggtcgtagcc ttccggatgg gccaaggtga
    10741 catccatacc gaagcgagtc atcagaccga tgatgccttg tggtacagag agcggtttgc
    10801 catagcttgg agagtaggcc caggtcatgg cgattttttt acctttcagg ttttccagtg
    10861 aaccaaagtg ttcacgtaac cacgcgaggt cagccattga ctgagtcggg tggtcaatat
    10921 cgcattgcag gttcactaaa gccggacgct gtggcagtac accctgcttg taaccgtcat
    10981 caagtgcagc gccaacttca cgcatatagg cgttgcctgc gcccagatac atatcgtcgc
    11041 gaataccaat agcgtcggcg cagaaggaga tcatattggc ggtttcacgc acggtttcgc
    11101 cgtgagcgat ttgtgatttg ccttcgtcga gatcttgttg tgcaaggccg agcaggttaa
    11161 gcgcggaagc ataagagaag cgggtacggg tggagttgtc gcggaatacg gaaataccta
    11221 atccactatt aaagactttg gttgagatgt tttcagcacg cagtgctttt aatgctgcgg
    11281 caacgtccag tacttgtttc agttcatctg gcgtctgttc ccacgttaac aaaaaatctt
    11341 tctcgtgaag gtgagaggtc agcgaattga tatccttaat cagctcatta acagttttca
    11401 tcatacagtc ccggtagtgg aatgacaggc aaagtggttt atttcactgt gacttgccaa
    11461 agtgattggt gttttcattg ctatacctgg gactaacaat aagtgtgcca gaattatccg
    11521 cgcggctgca aaagactaat ttgacggata atgtgtgggg ttgttcacaa attgatgcca
    11581 gcttctggtt gtttgtaaaa tacgaaaacg tttggctttt tttatcgcgt tatttatttt
    11641 ctgctaagta tcagaaataa gataaagggg caggctatct ctcttcagtt tgataaactc
    11701 accgccgtaa ttctcatatt gatatagaaa tgtgatatct cttccataat tgcattagaa
    11761 aatgataaaa atcttatgtt tttatcaaag ccaatttcgc ctgagttaat atcatcatga
    11821 tctgtgaacc atcaacgtct tcaatgatcg acttttaagt aagtctggaa ggtaaagcta
    11881 tggagcttgc tactacgcag tcagtattga tgcaaattca accgacaatt cagcgttttg
    11941 ccagaatgct tgccagcgtt ttgcagtttg aggttgagat cgttgatgaa aacttgtgtc
    12001 gcgtcgccgg aacgggcgcg tatgggaagt ttcttggtcg ccagttgagc ggcaactcac
    12061 gcctgctccg ccacgtcctg gaaacgaaaa ctgaaaaagt tgtgacacag tctcgcttcg
    12121 atcccctttg cgaaggttgc gatagtaaag aaaattgccg cgaaaaagca tttctgggta
    12181 cgcctgtcat tttacaggat cgttgtgttg gggtgataag tttgattgcc gttacccacg
    12241 agcaacaaga gcatatcagt gataatttac gcgaattttc tgattatgtt cgccatatat
    12301 ccaccatttt tgtttcgaaa cttctggagg atcaggggcc aggagataac atcagtaaaa
    12361 tattcgcgac catgatcgat aatatggatc agggcgtatt agttgttgat gatgaaagtc
    12421 gggttcagtt tgttaatcag actgccttaa aaacacttgg tgttgtacaa aataatatta
    12481 ttgggaaacc tatccgtttc agaccattaa catttgagag taattttact catggacata
    12541 tgcagcatat tgtttcgtgg gacgataaaa gtgaattaat cattggtcaa ttgcataaca
    12601 ttcagggccg acaattattt ttaatggcat ttcaccaatc gcataccagt ttttctgtag
    12661 caaatgcacc tgatgaacca catattgaac aattggttgg cgagtgccgt gttatgcggc
    12721 aattaaaacg actcattagc cgtattgcac ccagcccatc cagcgttatg gtggttggtg
    12781 aaagcggcac gggtaaagaa gtcgtcgccc gagcaatcca taagttgagc ggaagacgga
    12841 ataaaccctt tattgctatc aactgtgccg cgattccgga gcagcttctg gaaagcgaac
    12901 tgttcggtta tgttaaaggc gcatttactg gtgcttctgc caacggtaaa acagggttga
    12961 ttcaggcggc gaatacgggc acgctgtttc tcgatgaaat aggtgatatg ccattaatgt
    13021 tgcaggctaa attactgcgc gctattgagg cgcgtgaaat tctgccgatt ggtgccagta
    13081 gcccaataca agtcgacatt cgcatcattt ctgcaactaa tcagaatttg gcccagttca
    13141 ttgccgaagg taaattccgc gaagatctct tctaccgact taatgttatc ccgataactc
    13201 tgccaccgct gcgtgaacgt caggaagata ttgaactatt ggtgcattac tttttacatc
    13261 tgcatacccg tcgtctggga tcggtttatc ctggcattgc tcccgatgtc gtcgaaatat
    13321 tgcgtaagca tcgttggccc ggaaacctgc gcgagttaag caatttgatg gaatatctgg
    13381 ttaacgtggt tccttcaggt gaagttatcg acagcacgct attgccgcca aatctgctga
    13441 ataatggcac aacggagcaa agtgatgtaa cagaggtcag tgaggcgcac ctgtcactcg
    13501 atgatgcggg cggcacggcg ctggaggaga tggaaaagca aatgatccgc gaggcgcttt
    13561 cacgtcataa cagcaagaag caagttgctg atgaactggg catcggcatt gctacgctct
    13621 atcgcaagat taagaaatat gagttgttaa acacataacc tcagtgaatc cggcctgaat
    13681 tcaggccgga tatcctttta cttcgttttc tcgcaatcca gaactgtatt tacaatcatc
    13741 tgataccccg tgcagcgaca aagatttccc gccagtccgc gacgaatttc cgtaatggtt
    13801 aatggcttct cgcgtggttt cgccagcatt gccgtggtag ccataatcag gccaggcgta
    13861 caaaacccgc actgcactgc gccggatttc gcataagcct gctgaacatg agaaagtttt
    13921 ccgcctttcg cttcaccttc cagcgtgcgg atctcctttc cttcagccca ggcggcaagg
    13981 tataagcaac tgtctattgc tgtgccgtcg accaacaccg tacaggcacc acattcaccc
    14041 acgcagcacc cttgtttgac acttagcagt ccttgttcgc ggagtaattc cgagagcggc
    14101 gtgcctggtg cggcgtgaag ctgaaaaggc atcccgttaa tggtgcattc gatggtaatt
    14161 gtttcgctgt gattcattgc aattttcccc ccgccgcggc gacggcttcg ctaatcactt
    14221 ttttggtcat cgtctggatg agatgcagac gaaactcttt actggcccgc catgaagaac
    14281 gcggggcgac atcttgcagg acagattcgc tgatagcttc cagcgtttgc aggtttaatg
    14341 gcgcattttg tgcagtctgt tcggcatgtt ggcagcgaat cggcgttggc gcggcaacac
    14401 caaatgccag gcgtaattcg ctgaaattgc cgttatccag tcggcaatgt gcggcgcagc
    14461 caatcgttga aatatccatt gcgtcgcgca tggcatattt aaaatgcgcg ctgcccgcgt
    14521 gttctttcgg ctgtggcgga aaatgaaagg cgacgaggat ttcgtcatgc tcaagagaca
    14581 ctttgcccgg cccggtgtga aagccattaa tcgggacgaa acgaacaccg cgcggggagt
    14641 ggatctccag tttcgcgtca taaattagcg ttggcgtggc agaatctgcg ctggtggcac
    14701 cgttgcaaat atttccaccg taggtagcga cgttacggat ctgcggtcca gcaatggacg
    14761 tggccgcagc acataacgcc gggagatgac gttgagttat aggagcttct attagctggg
    14821 taaatgtcgt tgcagagccg atacgtagcg agccatcttc cgccagcgta attccccgca
    14881 gctccgccag attatgaata tcaacaatat ggcgataacg gtcattgtga tggtggagct
    14941 gaatcagtac gtcagtgcca tcggcgagca gtttggcctg cgggttgtca gccagcaggt
    15001 tgatggcatc ggcaagggtt gctgcgcgat ggtaagaagc aaaatcaaac atgatgttat
    15061 cctcaaatca atcctgccag atggaactct tcatataacc gttttggcgt cagcggcagt
    15121 gtattgattg caacaccggt agccatcttc accgcgttac gaatagcagc ggcaacagga
    15181 attattggtg gctcacccag tgacttatgt ccgtatgcgg attgcggctc attgatttcg
    15241 acgaacgcgc tttccagttg tggcagatcc ggcatggtcg gcattttgta atccagcaga
    15301 ttggggttac ggaccacgcc gcttttagca tcgatgatca tctcttcaaa tagcgcccag
    15361 ccaatgccca ttcccattcc gccgtgtacc tgaccttctg ccagcagtgg attaagaata
    15421 tgccctgaat catgaacgtt gaggatgcgg ttgatggtga ctttgcacag cgcaatatcg
    15481 accgtcagat caacaaaggt acagccaaac gccgatgggt tagtggtggt tttgatggag
    15541 ctttcagcag agagctgccc gccgcgttca gggtggtaga aagcgtccat cgccaaatct
    15601 tttaacgaca ttaacggctc ttccggtcgt tcaaccagca cgatatggcc ttttatcagg
    15661 gtcagattca tcgctgactg atgtagcatg actgcggcgt gagcgatgat tttctctttt
    15721 aataatagtg ccgcactgcg cagcgcaggc gcggcaacat agctctggcg tgaggcaaat
    15781 gcgccgggag cgaacggcgt aacgtcggta tcttgagttg aaataacgcg aacgtcgctg
    15841 accggaaccc ccacggtttc tgccaccatt tgcgagaaga cggtgtcggc accctgaccg
    15901 atttccgtcg cgccgctttg cacgttgatg gttccatcct gattcatcag aaggcgcgcg
    15961 cctgctattt ctacgccgac aggccaggtg ttagaggtat agctaaaaca ggcgacgcca
    16021 acgccgcggc gcaaattgcc ttgctggttc tggcattctg cacggcgttt ttcccattca
    16081 aagattttcc ggcctttttc aagacactcc ggcaaccctg cgctgtaaat acgtttgccc
    16141 gtgagcggat tagcatctcc ttcgcgggcg gcgttgcgta aacgaatttc aacaggatca
    16201 atacctaacg ctgtcgcggc gtcatcaagc atagactcaa cggcaaatac gacttgtggc
    16261 gcgccataac cacgcatcgc accagccgag gggaggttgg tatagcaggt ctttgaactg
    16321 taagcgtagg cacaacgagg ataaaggtaa gcgactttat tcctccccgc agaagcgatg
    16381 gagtgcccgt gagatgcata agcgccggtg ttagacagaa catccagact ataacctttc
    16441 aatgttccgt cgcggttcac gcccatttgc ccgtcaatgg taaaagcgtg gcgggtacgg
    16501 gttgcgagga aacactcttc acggctaagg gaaactttca ccggaatgcc gccaagcttg
    16561 ctggtcagga atgctgccat tggctcttcc agtacatcct gtttattacc aaaaccgcca
    16621 ccgacaaacg gtttgatgac tcgtacgcat gaccagggaa tatccagcgc ctgaccaacc
    16681 acgcggcgaa caatgtgcgg gatctgggtg ctggaaacga tggtaattcg cgagtcatcc
    16741 tccatccacg ccagcgatgt tacgctttcc atgtgacaat gttgaataac gggggtctga
    16801 tagtgcccct gtacctggta gtcggcggca tcgattgttt gttggacatt acccgtcgac
    16861 atcgtgcttt gtttcagtaa attgccaccg ttatggattg gtgcagcgtc ttctgccagc
    16921 gccgcttctg gcgtggtgat aacgggtaat tcttgccact caatgctgac caattgcgcc
    16981 gctttttctg ccgtgagttc atcgcgggcc acgacgatgg caacggcgtc accatgatga
    17041 cgaacatggc gagttagcag tgcgcgatcg gcggtatcgc gcttgttttc gtcaagtgtc
    17101 caggcatgcc cagctgtagc gaatggaata tcaggcacat cttcccaggt aaaaatcgcc
    17161 agtacgcccg gcaaacttct ggcttgttca tcattaatac ttacggcata accatgtgcg
    17221 atagggctac gtacatattt cgcataacac atgcccgcca taacataatc gtcagtatat
    17281 cgtgcccgcc cggtgacctt agcaatggca tcgacgcgca tgcatgattc acccgtagcg
    17341 gttgcttccc gcgcttccat agaaatcccc tcgatttgaa catttggttc ttatggcaaa
    17401 gatttagtct aagcaggcaa gatttacgcc agaaacgacg ataagcgtta ggggaggtag
    17461 gaagtgtgat gacgttaaaa aaacacagat atgatacgaa acaggactct gaataggggc
    17521 agtgatacgt atcaatatga gtggcgataa aatcaatgtg ataaattggg attattacgc
    17581 atcaggatgg gaaatagggt aaaaagtcag gaagttcgat acctctacag cgaatatttt
    17641 gctgatgata cttccctttg ccaaatgaaa ttaacctctt tctctattac cacgtttttc
    17701 cagaagcaag agattgggta tcaagaggct ggctgctatg ataaggcgtc ttgtttttta
    17761 agcgaggaaa gattttgagt gcgggacgcc tgaataaaaa atctctgggt atcgtgatgt
    17821 tgttatcggt tggactgctt ttggcgggct gttcgggtag caaatcatcc gatacaggaa
    17881 cgtattccgg ctccgtttac accgtgaaac ggggggatac gctatatcgt atttcgcgca
    17941 ccacgggaac cagcgtaaaa gaactggcgc gactgaacgg catttccccc ccttacacca
    18001 ttgaagttgg tcagaaacta aaactgggtg gggcgaaaag tagcagtagt acacgtaaat
    18061 caaccgccaa atcaacgacc aaaaccgcat cggttacacc gtcatcagcg gtaccgaaat
    18121 catcctggcc gccagtaggg caacgttgtt ggttatggcc aacgacaggg aaagttatca
    18181 tgccgtattc gacagcagat ggcggcaata aagggattga tatctcagct ccacggggta
    18241 cacctattta cgccgcgggt gcaggaaagg tggtgtatgt gggcaaccag ctgcgtggct
    18301 acggtaatct catcatgatt aaacacagtg aagattacat tacggcttac gcccataatg
    18361 acacgatgct ggtaaataat gggcaaagcg tgaaggctgg gcaaaaaatc gccactatgg
    18421 ggagcacgga tgcggcatct gttcgcctgc atttccagat tcgttaccgt gcaacggcaa
    18481 ttgatccgct acgttacttg ccgccgcagg gcagcaagcc aaaatgctga tggcgaatta
    18541 atcagcagtc agcagcgcgg cacttgctaa ggagagcgta aggtttataa tgccttacgc
    18601 atctcgaagc gggcgtagtt caatggtaga acgagagctt cccaagctct atacgagggt
    18661 tcgattccct tcgccgcttt tgctgtatct gattttctat attatcttaa ttcaatcgct
    18721 tcagactttt cgcaatcgcc gtaatatttc cccattttgt tgtaaataaa aagcgtttac
    18781 gcaatgcaat ccttttgtaa caacccaggt aaaacacctt attttcaagt tgttaaataa
    18841 gtggttattt acgcttttaa caaacgattg atggaatatt ttatgaaagg gaaatctgca
    18901 ttaactcttc tgctcgctgg tatattttcg tgcggcacat gccaggcgac aggggcagaa
    18961 gtgacgagtg aatctgtatt taacattctt aattccacgg gggcagcaac agataaaagt
    19021 tatttatcgt taaatccaga taagtaccct aattatcgtc ttctgattca ctcagccaaa
    19081 ttaaaaaatg aaataaagag ccactatacc aaagacgaaa ttcaagggtt gttaacatta
    19141 acagaaaaca cacgaaagtt aacgttgacg gagaaaccct ggggaacatt tattcttgct
    19201 tctactttcg aagatgacaa aacagcggca gaaacccact atgatgctgt ctggctgagg
    19261 gatagtttat ggggctatat ggcattagta tcagatcagg gaaatagtgt agccgcaaaa
    19321 aaggttctgc tcaccctgtg ggattatatg tctacgctcg accagattaa gcggatgcag
    19381 gacgtcatta gcaaccccaa acgactggat ggagttcccg ggcaaatgaa tgctgtgcat
    19441 atccgatttg acagcaactc ccccgtgatg gcagatgtgc aggaagaagg caagccgcag
    19501 ctgtggaacc ataaacaaaa tgatgcgtta ggtctttatc ttgatctcct tatacaagca
    19561 atcgacactg gtacaattaa cgctgaagat tggcaaaaag gtgaccgatt aaaatcggtt
    19621 gcgctgctga ttgcttatct ggataaagcc aatttctatg ttatggaaga ttccggagca
    19681 tgggaagaag atgcccgatt aaacacatct tccgttgcac tggtgacctc tggactggaa
    19741 cgtttgtcga acttactctc gaagaaagac tctgtttttg tctctgattt actacgcgaa
    19801 gctaaagcca atgaactgga tgaacctctt tcaacaacga ggttgaacca cctcatagat
    19861 aaaggttatg aaagaataac gttacagctt gatttagggg gagaatcccc aggttatctg
    19921 gaaaaagata aacattacag agaagccgat gcggctttac ttaatgttat ttatcctgca
    19981 aatcttgcaa agataaacac cagaagaaaa gagcaggttc taaaaatagt taaaaaacta
    20041 gcaggtccgt atggtataaa aagatacgag aaggataatt atcagtcggc aaatttttgg
    20101 ttcaatgata ttaaaaccga taccgatcaa aattcacatg cgaaaag
//
LOCUS       DAETQN010000077        20077 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.077, whole genome shotgun sequence.
ACCESSION   DAETQN010000077 DAETQN010000000
VERSION     DAETQN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.077"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            59..568
                     /gene="radC"
                     /locus_tag="K0W68_002745"
                     /pseudo
     CDS             59..568
                     /gene="radC"
                     /locus_tag="K0W68_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            785..1021
                     /gene="rpmB"
                     /locus_tag="K0W68_002746"
     CDS             785..1021
                     /gene="rpmB"
                     /locus_tag="K0W68_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBI3074264.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            1042..1209
                     /gene="rpmG"
                     /locus_tag="K0W68_002747"
     CDS             1042..1209
                     /gene="rpmG"
                     /locus_tag="K0W68_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBI3074265.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1307..2116
                     /gene="mutM"
                     /locus_tag="K0W68_002748"
     CDS             1307..2116
                     /gene="mutM"
                     /locus_tag="K0W68_002748"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBI3074266.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="K0W68_002749"
     CDS             complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="K0W68_002749"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBI3074267.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="K0W68_002750"
     CDS             complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="K0W68_002750"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBI3074268.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4332..5390
                     /gene="rfaQ"
                     /locus_tag="K0W68_002751"
     CDS             4332..5390
                     /gene="rfaQ"
                     /locus_tag="K0W68_002751"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBI3074269.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5387..6511
                     /locus_tag="K0W68_002752"
     CDS             5387..6511
                     /locus_tag="K0W68_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBI3074270.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6504..7301
                     /gene="rfaP"
                     /locus_tag="K0W68_002753"
     CDS             6504..7301
                     /gene="rfaP"
                     /locus_tag="K0W68_002753"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBI3074271.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7317..8333
                     /gene="waaO"
                     /locus_tag="K0W68_002754"
                     /gene_synonym="rfaI"
     CDS             7317..8333
                     /gene="waaO"
                     /locus_tag="K0W68_002754"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HBI3074272.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNFKQNEPINGIMNYIYYFYL
                     KIIK"
     gene            8350..9345
                     /locus_tag="K0W68_002755"
     CDS             8350..9345
                     /locus_tag="K0W68_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HBI3074273.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNIKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            9355..10047
                     /gene="rfaY"
                     /locus_tag="K0W68_002756"
     CDS             9355..10047
                     /gene="rfaY"
                     /locus_tag="K0W68_002756"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBI3074274.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYCRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            10073..11098
                     /locus_tag="K0W68_002757"
     CDS             10073..11098
                     /locus_tag="K0W68_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HBI3074275.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            11183..12166
                     /locus_tag="K0W68_002758"
     CDS             11183..12166
                     /locus_tag="K0W68_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBI3074276.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            12212..13465
                     /locus_tag="K0W68_002759"
     CDS             12212..13465
                     /locus_tag="K0W68_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBI3074277.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNNLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(13535..14506)
                     /gene="rfaC"
                     /locus_tag="K0W68_002760"
     CDS             complement(13535..14506)
                     /gene="rfaC"
                     /locus_tag="K0W68_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBI3074278.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(14510..15556)
                     /gene="rfaF"
                     /locus_tag="K0W68_002761"
     CDS             complement(14510..15556)
                     /gene="rfaF"
                     /locus_tag="K0W68_002761"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBI3074279.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(15566..16498)
                     /gene="rfaD"
                     /locus_tag="K0W68_002762"
     CDS             complement(15566..16498)
                     /gene="rfaD"
                     /locus_tag="K0W68_002762"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBI3074280.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            16712..17908
                     /gene="kbl"
                     /locus_tag="K0W68_002763"
     CDS             16712..17908
                     /gene="kbl"
                     /locus_tag="K0W68_002763"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBI3074281.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            17918..18943
                     /gene="tdh"
                     /locus_tag="K0W68_002764"
     CDS             17918..18943
                     /gene="tdh"
                     /locus_tag="K0W68_002764"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBI3074282.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            19182..>20077
                     /gene="waaH"
                     /locus_tag="K0W68_002765"
     CDS             19182..>20077
                     /gene="waaH"
                     /locus_tag="K0W68_002765"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HBI3074283.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         5422 a         4377 c         4738 g         5540 t
ORIGIN      
        1 ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc
       61 attacctggc ttgttaacct ctgaatatga acaatttagt ggcgttcatg gaattggcgt
      121 agcgaaattt gcccagttaa aggggattgc tgaactggcg cggcgttact acaacgtgcg
      181 gatgcgtgaa gaaagccctt tactcagccc ggagatgacg cgggaatttt tacaaagcca
      241 gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactcac aacaccgggt
      301 tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga
      361 aattatccgg gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc
      421 ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag
      481 ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt
      541 ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc
      601 tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg
      661 ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta
      721 aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat
      781 agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc
      841 ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg
      901 ggttgagagc gaaaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat
      961 cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta
     1021 agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct
     1081 tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa
     1141 ctggaactga aaaaattcga tccagttgtt cgccagcacg tgatctacaa agaagcgaaa
     1201 atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat
     1261 ctgcttgccc ccatattgat tgcatctgtt cattcctgga gatgctatgc ctgaattacc
     1321 cgaagttgaa accagccgtc gcggcataga accgcatctc gttggtgcaa ccattcttca
     1381 tgcggtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag
     1441 cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ctgctgctgg agctgcctga
     1501 gggctggatt atcattcatt tggggatgtc tggcagcctg cgcatccttc cagaagaact
     1561 tccccctgaa aagcatgacc atgtggattt ggtgatgagc aacggcaaag tgctgcgcta
     1621 caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcacaacgt
     1681 gctggctcat cttggaccgg agccgcttag cgacgatttc aatggtgagt atctgcatca
     1741 gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt
     1801 aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg
     1861 gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt
     1921 gttgctgcgt tcgattgagc agggtggtac aacgctgaaa gattttctgc aaagtgatgg
     1981 taaaccgggc tatttcgcgc aggaattgca ggtttacgga cgaaaaggtg agccgtgtcg
     2041 ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg
     2101 gcagtgccag aagtaattca tacgcgccgg atggcatacc atccggcata aacgctacgc
     2161 taacttcgcc accagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg
     2221 atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat
     2281 cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc
     2341 atattcaaaa tccgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg
     2401 ggcgaagttc gccatcaaat cactaaaccc aaccacttcc acgttcccca gatgcgcggt
     2461 tgcctgctgt gtcagcgcca cacgctcttc cagggtaaac atcggttttt tactggggct
     2521 ggcggcaatc gccagaataa cgtgatcgaa catttgcgtg gcgcgcgtca cgatatcgat
     2581 atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac
     2641 ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc
     2701 cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt
     2761 cggtgagtaa agaggaaacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc
     2821 cgcttgcctg ctccagccgc gcgcaaatgt ctttgaaatt aaaagtatgc ggccccatca
     2881 ataccggaat agcgtgtgcg gcagcttcca gaggattatg ccctccacgt tcaaccagtg
     2941 aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat
     3001 cgccaaccac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc
     3061 ttagtccagc ctggcggaca aggttaatcg catccgggaa gcgttctgga tgacggggta
     3121 ccaggatgag caataaattc gggaattgct gtaataatgc ctgatgtgcg gcgatcacca
     3181 cactctcttc accttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact
     3241 ggcggcgcag cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca
     3301 ggctaccggt aacggttacc tggttatttt ttgcgcccag cgccacaaaa cgtgcaccat
     3361 cttcttcatt ttgcgcagca atcagcgtaa tacgacgcag taagcggcgg acgaatttac
     3421 ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg
     3481 gaattttacg tttatgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca
     3541 acaccagttt ggggtcgact ttattcagga aacggttgag cgcatcgggc aggtcatacg
     3601 gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg
     3661 gcgtcatggt tgtaacggta atcggtaaat caggataacg atggcgcagc gcgcgcacca
     3721 acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt
     3781 ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct
     3841 tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg
     3901 tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta
     3961 tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc
     4021 aattcttgct gaacatctat actttttttg tatttttatg cgattcattg atttctgctg
     4081 tacgtgcagg aaggaatcgc agagcaacag tgaaagaata tggttgctaa tgattttatt
     4141 tgattgattt ctttattgaa aatcaacaat gtgcttaaaa agcattcggg tcggattgcc
     4201 gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg
     4261 ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa
     4321 aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg
     4381 gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa
     4441 aaatcgatgt gctgctttat caggacacca ccccgatcct gtctgaaaat ccagagatta
     4501 acgcgctcta cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt
     4561 ttcatctcat caaggtatta cgtgccaata agtatgacct tatcgtcaat cttaccgatc
     4621 aatggatggt tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt
     4681 atcatcatcg gcagtctgct ttttggcgta aaagtttcac ccatttagtg ccgttgcagg
     4741 gtggaaatgt ggtggaaagt aacttatccg tgctgacacc attgggactt gattcgttgg
     4801 tgaagcagac aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg
     4861 atcacgctgg tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca
     4921 aatgctggga caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg
     4981 aagtcgttct gacgtccggc ccagataaag acgatctggc ctgcgtcaat gaaattgcgc
     5041 agggatgcca gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg
     5101 gtgcgttaat cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcgcatattg
     5161 ccgctgcagt taatacgccg ctgatatcgc tgtttggtgc gacagaccat attttctggc
     5221 gtccttggtc aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc
     5281 gcgatcagcg tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg
     5341 ctgctgtcga taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt
     5401 ttgtttatat aaatattttc cctttggcgg tttgcagcgc gattttatgc gtattgctca
     5461 gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg
     5521 ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa
     5581 tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt
     5641 tggattcaac aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga
     5701 gaaagtcgcg caggaaaaag gctttttcta tcacctgacg tcacgttatc gccattatgc
     5761 cgcctttgag cgggcaacct tcgaacaggg caagccgaca cagctgctga tgctgacaga
     5821 taagcaaatc gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct
     5881 gccaccgggg atttatcctg atcgtaaata tagccagcag ccagctaata gccgtgaaat
     5941 cttccgtaag aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga
     6001 cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct
     6061 gcgccacaac acattgctat atgttgttgg acaggataag ccgcgaaaat ttgaggcgct
     6121 ggcagaaaaa cgcggcgtgc gcagtaatgt tcacttcttc tcggggcgca acgatgtctc
     6181 ggaattaatg gcggcggcgg atttattact gcatcctgcc taccaggaag cagcgggaat
     6241 tgtgctactg gaagcgatta ctgcaggatt accggtacta acaacagccg tttgtggcta
     6301 tgcgcattat attgtcgacg ccaattgcgg cgaggctatt gctgagccat tccgccagga
     6361 aacattgaat gagattttac gcaaagcgtt aacgcaatct tcattgcgcc aggcctgggc
     6421 ggaaaatgcg cgacattatg ctgatacaca agatttatac agtctgccag agaaagcggc
     6481 ggatatcata acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc
     6541 gcggtaaaga tccttttgag gaagttaaaa ccttgcaggg tgaggttttt cgtgaactgg
     6601 aaactcgccg cactctgcga tttgaaatgg cgggcaaaag ctattttctc aaatggcatc
     6661 gcggcacgac cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag
     6721 gcgcagaccg cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgt
     6781 atggggtggc atttggcgaa aaaggtatta atccgctgag cagaacctcg tttattatta
     6841 ccgaagatct gaccccaacc atcagtctgg aagattactg tgctgactgg gcgaccaacc
     6901 caccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcgcgata
     6961 tgcatgctgt gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc
     7021 ctttttccgg taaggaagag gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc
     7081 ttcgcacgcg cgttccacgt cgttggcgcg ataaagatct tattgggctt tatttttctt
     7141 cgatgaatat cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgtcg
     7201 ccccgcttaa agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag
     7261 ccacaaaaat cagggaaaga acgattcgaa aatcgttgta atttccggag agaaatatga
     7321 gtgcccacta ttttaatcca caagagatga tcaataagac aatcatcttc gatgaaaggc
     7381 cagcggcgtc agtggcatca tcattccatg ttgcttatgg cattgataaa aactttcttt
     7441 ttggttgtgg tgtttcaatc acgtcagttt tgttacataa caacgacgtg agttttgttt
     7501 tccacgtttt tattgatgat atccctgaag ccgatatcca gcgtttagcc caattggcga
     7561 aaagctatcg tacctgtatc cagatccatc tggtaaattg tgaacggctt aaggcattac
     7621 cgacgaccaa aaattggtct attgccatgt atttccgttt tgtaattgca gattacttta
     7681 ttgatcaaca agataagatc ctttacctgg atgctgatat cgcctgtcag ggaaacttaa
     7741 agccgctgat aacaatggat cttgccaata acgttgctgc tgttgttact gaacgcgatg
     7801 ctaactggtg gtcgttacgg ggtcaaagtc tgcagtgtaa tgaacttgaa aagggttact
     7861 ttaattcagg tgtcctgtta attaatacac tagcgtgggc gcaggagtcc gtttctgcta
     7921 aagcgatgtc gatgcttgct gataaagccg tcgtttcccg tttaacctat atggatcaag
     7981 atatacttaa tcttatcctg tcagggaaag ttaaattcat tgatgctaaa tacaatactc
     8041 aatttagttt aaattatgaa ttaaaaaaat catttgtttg tccaattaat gatgaaaccg
     8101 tattaattca ttatgtcggc ccgacaaaac cctggcatta ctgggccggt tatccaagtg
     8161 cgcaaccttt tatcaacgcc aaagaagcat cgccctggaa aaatgaaccg tttatgcggc
     8221 caattaattc aaactatgct cgttattgcg ccaagcataa ttttaaacaa aatgaaccaa
     8281 ttaacgggat aatgaattat atttattatt tttatttaaa gataataaaa tgatattgag
     8341 aaaagttaaa tgaatgaatt tataaaagaa cggttttcgt atttagcaga taataaaaaa
     8401 gaaaacgccc cagaactaaa tgtttcctac ggtatcgata agaatttttt gtatggtgct
     8461 ggcgtttcaa tttcttccgt tttgattaat aattcagata ttaattttgt ctttcatgtt
     8521 ttcactgatt atgtggatga tgattattta aagtcattta atgaaacagc aaaacaattt
     8581 aatacctcaa ttattgtata tttaattgac ccaaaatact ttgctgatct gccgacgtca
     8641 cagttttggt cgtacgcgac atacttcagg gtattgtcct ttgaatatct gagtgaaagt
     8701 atttccacac tgctgtatct ggatgccgat gtcgtttgta aaggaagtct gaagcctctc
     8761 acagaaatta tatttaaaga tgagtttgct gcggtcattc ctgacaatgt tagtactcag
     8821 gcggcatgtg caaaacgcct caacattccc gagatgaatg gacgttattt caatgcaggc
     8881 gttatctatg tcaatattaa aaaatggcat gaagcaaatt tgacaccgta tttactcacg
     8941 cttttacgag gggaaactaa atatggctct cttaaatatt tagatcagga tgcgttgaat
     9001 atcgcattta atatgaataa tatctacctc gcgaaggatt ttgatactat ttataccctg
     9061 aaaaacgaac ttcatgatcg tagtcatcga aagtatcagc aaaccattac cgataaaaca
     9121 gtattgattc actatacagg gataactaaa ccatggcata gctgggctgg ttatccgtct
     9181 gcatcatact ttaatatcgc gcgtgaacaa tctccctgga agaaatatcc tcttaaagag
     9241 gcgcggactg ttgcagaaat gcagaaacaa tataagcatc tgtttgccca tggtgagtat
     9301 attaaaggca taacttcatt aattaagtac aagcttaaga aataaattac tcttatgatt
     9361 acaagtatac gctatcgcgg cttctcattt tattacaaag ataatgataa taaatataaa
     9421 gaaatctttg atgaaatact ggcctataat tttaaaacag taaaagtatt acgtaatatt
     9481 aatgatacca aagtatcatt gattgatacg aaatattgtc gctatgtttt taaagtcttt
     9541 gccccgaaaa cgaaaaggaa tgaacgtttt ttaaaatctt ttgtaaaagg tgactactat
     9601 cagaatttga tcgttgaaac cgatcgtgtc agaagtgcag gtcttacttt tcctaatgat
     9661 ttttattttc tggctgagcg taaaattttc aactatgcca gtgtttttat catgcttatc
     9721 gagtatgtcg agggcgtaga gctcaatgat atgccgatca ttccagaaga tgttaaagca
     9781 gaaattaagg cttcaatgga aaaattgcat gccttaaata tgctatcggg cgacccacac
     9841 cggggtaact ttatcgttag taaagatggt gtcaggataa ttgacctatc cggtaaaagc
     9901 tgtacggcag agcgtaaagc tcgtgatcgt ctggccatgg aacgtcacct gggtattgcc
     9961 aacgaaataa aagattatgg ctactattca gtaatctaca gaaccaaact acgcaaattt
    10021 attaaaaaat taaaaggcaa agcgtaaacc acacagtcaa aacggaacca acatggattt
    10081 attagctgag agtattactg aagtcgctgt ctctggggaa attgctaaca ccgatcgtgt
    10141 gttaaatatc gcttacggta ttgaccgtaa ctttttattt ggtgcggcag tatctatgca
    10201 atcagttgtt atgcataatc cggaccttgc ggttaagttt catctcttta ccgactacat
    10261 tgatgaagat tatctacaac gtgttaatgc ttttaccagc aaaaatgcta acgttgaagt
    10321 aagaatttat aaagtctcca gtgcctttat tgatatcttc cccagcctga aacagtggtc
    10381 ttatgcaaca ttcttccgtt tagttgcgtt ccagtatctg agtgaaacta ttgaaaatct
    10441 gttatatatc gatgctgatg tcatctgtaa aggctcatta gctggattgc ttgatattaa
    10501 ttttgatggg gataagtttg cagctgttat taaagatgtg ccttttatgc aggaaaaacc
    10561 agcgaagcgt ctggctatag agggacttcc agggaattat ttcaacgccg gtgtagtata
    10621 tctgcagctt gaagcatggg cgaaaaatga ttttatgaat aaagccattg ctatgctggc
    10681 aagtgacccg cagcacacga agtataaatg ccttgatcag gatattttaa atattctgtt
    10741 ctttggtcat tgtattttta ttagcggcga ttatgattgc ttttatggca tcgattatga
    10801 gttaaaaaat aaaagcgatg aagattataa aaagaccatt accgatgata ctaagctgat
    10861 tcattatgtt ggcgtaacga agccctggaa cgactggacg aattatccct gccagaagta
    10921 ttttaatgag gcttatcagg cttcttgctg gaatgatgtg gcgtttattc cagccacgaa
    10981 tgaaaagcag tatcaagtga aatatcaaca tgcaaagaaa aatggtgata cgtttaacgc
    11041 ttttatttac tttattaaat ttaaattaaa taagtataaa agaaaactat ttggataata
    11101 ataaagatca tggatgtctg agttacaata ataattaagg atgagtacta tcatatttta
    11161 ttagttcagg caagagaaca cgatgagcaa tgattaccct ttagtatcca taataatacc
    11221 gacgtataat tcatctgatt acattactga aactctaacg aaattagaaa aacaaactta
    11281 cccaaatttt gaaattgtta ttgttaatga tggttctaaa gataacacat caaacgtttt
    11341 gagagagtat gggttaaccc actctcgatt aattattatc aataaagaaa atggcggtgt
    11401 ttcgtctgcc aggaatacag gtatccgcaa ggcgcaagga cagtttatat gttttatgga
    11461 tgatgatgat gagatagatc ctaactatct gctgaagatg tataccagac aacatgagac
    11521 gggaggggat gcgatttatt gtgggcttta tggccattat ataaaagatg gtgttactta
    11581 ctcacctata aatacagagt ttaatgaagg atctttactt ttcgactttt tttataaaaa
    11641 ggttagattc catatagggt gcttgtttat aagaaaacaa cttctggaag agaataatct
    11701 tttttttgat gaagatttac gactaggaga agatctggat tttatctatc gactgctaat
    11761 tacatgcgat atgtatgcgg ttccatatta tatgtataag cataactata gagaaaattc
    11821 cttaatgaac tcatgtagaa ccatcactca ttatcgacat gagtcatttg cgcacgaaag
    11881 aatctactct tctgtgatgc agttatacaa aggtaacagg aaagaagaaa ttcatacatt
    11941 attgagtcaa aatagagctt atcataaaac tcgttattta tggaatgttc tacttaatgg
    12001 tgattttgaa ctattaaatc aattagttga aagcaatgaa aaagaattga atgattgtaa
    12061 tctttctggc aagagagata aaagacgagc aaaaatatta gcatcaaaaa attatattct
    12121 ctggaggatg gtaagactgg taaataggaa aaagaataaa cgttagatct tgcattaaat
    12181 aatccgtcct cgtagcatag gttgaaacta tatgtcattt tgttggaatg aaattaattc
    12241 tggtataaag tctttaattc tcatattatg tattttttct ttgatgactt tgtctttatg
    12301 ggatgatgtt gcaacaaagt ttcttcatgc agctggaatt atatctgcat tgtattttct
    12361 aataacacca aaaaaaacaa taactaacaa tcctactttg ttaattttca tctcattatg
    12421 tcttttggga atcataaata tcatctggta ttcacattat aaagtttcag gctctgttta
    12481 taccaatgca tatcgtggcc caatggaaac tgggaaaatt gccttgtgta gtgcttttat
    12541 tttcttggtt ctttttgcta aaaatgagtt gagaacaaaa ataaaatttg ggaaactaat
    12601 tctgttcgca tccctggcaa cgcagttact tttttttgcg catgccatgt ggcaacattt
    12661 ctatttaaac gttgaccgtg ttgcattatc agcttcccac gctacaacag caggctacat
    12721 catccttttc ccttctttac tggcatcaat tctcatttta aaatccgact ttagacataa
    12781 aacaacatta tatacaatta acttcatgct tagcttatgt gctgtcatag taactgaaac
    12841 gcgtgcagcc atattagtgt ttccattctt tgcgttacta ttaatcgtaa tggatagtta
    12901 tattaataag cgaattaatt ataagttata ttgttttatt gcgattgcat tattagcagg
    12961 tgtattttct tttaaagata cattgcttat gagaatgaat aacttaaata acgatttagt
    13021 taattattcg catgataaca ccagaacttc agtcggtgcc cgtctggcaa tgtatgaagt
    13081 tggcttaaaa acatattctc caataggaca atcactggaa aaacgagcgg aaaaaataca
    13141 tgaactagaa gaaaaagagc ctagattgag tggcgcttta ccctatatag attctcattt
    13201 gcataacgat ctcatagata cgttatcaac gcgtggtatt cctggagttg tattaacaat
    13261 tttagccttt tcagcaatac tcatatatgc cttaagaact gctaaagaac cttatatttt
    13321 aatcttgctt ttttcactac tggtagtagg cctaagtgat gtaatactct tttctaaacc
    13381 ggttccgact gctgtgtttg tcaccataat attgctttgt gcttatttta aagcacaatc
    13441 agaccaatgt ttattagata aataatgaat atgtgaaata aaatcagcaa ggaggctgat
    13501 tttatttcaa aattatctat taaggggttg taatttaaat catagcaacg ttttcttcaa
    13561 tgaatcgctt aacagcattt gctgtcaatt gagacaactc attccccgga gccctacaaa
    13621 ccatctgatt cttcccatat ccaccaatta atcccggatc ggttggtcca taaaccgtga
    13681 tattgggtct atccagtgcc gccgttaaat ggcttaaccc cgtatccacc gacactacaa
    13741 atttagcccc ggccagcacg cgggcaacgc cttccagact catcttcggc aatacttcaa
    13801 cataagcaaa tccttccgcc agtcgtttcg cccgctcttc ctcatgcggc gcgccccacg
    13861 gaagtttaat ccgtattcct gaatcagcca gtaaaccaat caattctcgc cagtgttctt
    13921 ccggccagtg tttatcatca cgggtcgtcg catgaagaaa tacggcatat tcgccagcat
    13981 ctgtaggcag gttcgtcagg aaatgctgtg caatagcata atcgccctgg gtttgcggtt
    14041 tgctatagcc cagacttttg gcaaacagtt cgcgggtgcg ttctacggcg tgctgctgtt
    14101 ttgcaatatg atgcttacga ttgtaaaaca gactggctaa aggttcgcga gcggtttgcc
    14161 agtccatgcc atgctttacg ccatgcgcca gacgcgtcac cagcgccgcg ctttttacca
    14221 gcccctgagc gtcgataact gcgtcatagt tctctgcttg tagcgcttca cgaaacgctt
    14281 tgcgttccgc ttttatgggg gccgagaacc aggctttacg ccagcgacgt attgccacag
    14341 gaataactcg ctcaacggcg gcgtgccagg aaggaatctg tgcgaaccct tcttccacca
    14401 cccagtcaaa cttaatccct gggattgcct gctgggcatc agtgagtgcg ggcaacgtat
    14461 ggagaacatc gcccatcgac gatgttttaa cgatcaaaac ccgcatccgt caggcttcct
    14521 cttgtaacaa tagcgcgttg agttcttcca gtacgcgctg gggagtaatg tcgattaagc
    14581 tctggtgata accctccgca gcgtcacctt tacgcacttt gtgatagccg gtaatcaggc
    14641 ggatcacgcg cgctttatgg gatagcggcg gtgtgaagtc cgggctactc ggaccataca
    14701 gggcaaccag cggacgattg agcgccgccg caacgtgcat caggccagaa tcgttagtga
    14761 caatggcttt acaggctgca atcaggataa ccgcttgatc aagctgtgtt tcccccgcca
    14821 ggttccgaca ccatgcctgc tgctcggtat tcaaagcggc aagaatctca ttgcccgctt
    14881 catgatcttt cgccgagcca aacagaacca cctgataacc ttcatcaatc agctgctttg
    14941 ccagttccgc atagtggtag tgtggccagc gttttgccgg accaaactcc gcacccgggc
    15001 aaaaaccaat catcggacgt tctgatgaaa gcgaaaattg attacaggta tatgattttt
    15061 caccttcgct cacctgcaat tgcggccata acaacggttt tggtagatct tgtgcggtac
    15121 gcatcacgcc tttgtcatag gccagcgcga catagcgttc caccattagc ggccaggctt
    15181 ctttatcgag cacgcgtaaa tcgttgagta aaccgtagcg catctcgccg cgccagccgg
    15241 tgcgatgagg aatacccgcg aagaaaggca ctaatgcaga tttgaaggag ttgggtaaga
    15301 cgtaggcgcg gtcgtagcgc ttttcacgca ggctatgacc cagtttgcgg cgttcgccga
    15361 tttccagcgc tccgtgaccg agaggcatag gaatagcttc gttaacttcc ggcatccgcg
    15421 ataataatgg acggcaccat gccggtgcca tcacatcgat tatcgcctgg ggatagcgcg
    15481 cctggagcgt gcgatagaga ctttgcgaca tcatcatgtc gccaacccaa gacgggccga
    15541 tcaccagtat tttcatgcag agctcttatg cgtcgcgatt cagccaggcc atgtattccg
    15601 ttacaccttc agcaacggtt ttgaacggtt tgtcgtaacc cgccgcgcgc agatttgtca
    15661 gatctgcctg agtgaacgcc tggtagcggc ctttcagctt atccgggaac ggaatgtatt
    15721 cgatctggcc tttcttgtga taagccagcg tagcatcagc tacagcctgg aaggattccg
    15781 cacgaccagt accgaggttg aagatgccgg aaacgccatt ttccaggaac cacagattca
    15841 catcagccac gtcgcccaca tagacgaagt cgcgtttgaa gttctcgcta ccttcaaaca
    15901 gcttcggtga ttcaccgttg ttaagctggg tgttgagatg gaaagcgacg ctcgccatgc
    15961 tgcctttatg gccttcacgc ggtccataaa cgttgaaata gcggaagcca acaatctgcg
    16021 agttagcttc aggcaggatt tggcgaacat attcatcaaa caggaatttt gagtaaccgt
    16081 agacgttcaa cggtttttcg tactcgcggg attcaataaa gtcggaggtg cgtccgccgt
    16141 aggtggctgc ggaagaggca tacaggaacg ggatttcgcg ctccagacag tagtgcagca
    16201 gctctttgga gtattgatag ttgttatcca tcatatactt gccatcccac tcggtggtgg
    16261 aagagcacgc gccttcgtgg aaaatcgctt cgacatcgcc gaactcttcg ccagccataa
    16321 tctggatcag gaagtcttcc ttatccatat agtccgcgat atccagatcc accaggttca
    16381 caaacttggt gccgtctttc aggttgtcca ccaccagaat atcggtgatg cctttatcat
    16441 tcagggcctt aacgatgttg ctgccgataa agcccgcgcc gccggtaacg atgatcataa
    16501 ctgtaacctt cgaattatgg agtcagagac aatctcagac acgaatactt ctatcatatc
    16561 actacacggc gttggcttca gccattcgcc aaaagtgaga ttgatggacg ttatttatcc
    16621 tgaattttgc agaagtgtta acgcgtcatc tcgtcgcgac ctataagttt gggtaatatg
    16681 tgctggaatt tgccctgtct ggagaatcgc aatgcgtgga gaattttatc agcagttaac
    16741 caacgatctg gaaaccgcac gggcggaagg gttgtttaaa gaagagcgca ttattacgtc
    16801 tgcgcagcaa gcagatatca ctgtggctga tggaagccac gtcattaact tttgtgccaa
    16861 taactatctc gggctggcga attatccgga tctgattgcg gcggcaaagg cgggaatgga
    16921 ttctcacggt ttcggcatgg cttcggtgcg ttttatttgc ggcactcagg acagccataa
    16981 agagcttgaa caaaaactgg cggcattcct ggggatggaa gatgcgattc tctactcttc
    17041 ctgctttgat gctaacggtg gcctgtttga aacgctgctg ggcgcggaag acgccattat
    17101 ctccgacgca ctgaaccacg cgtctattat tgatggtgtg cgtctgtgta aagctaaacg
    17161 ctatcgctat gccaacaacg atatgcagga gctggaagca cgtctgaaag aagcgcgtga
    17221 agccggtgcg cgtcatgtgc tgattgccac cgatggtgtg ttctcgatgg acggcgtgat
    17281 tgccaacctg aagggcgttt gcgatctcgc agataaatat gatgccctgg tgatggtaga
    17341 cgactcccac gcggtcggtt ttgtcggtga aaatggtcgt ggttcccatg aatactgcga
    17401 tgtgatgggc cgggtcgata ttatcaccgg cacgctcggt aaagcgctgg gcggggcttc
    17461 tggtggttat accgcggcgc gcaaagaagt ggttgagtgg ctgcgccagc gttctcgtcc
    17521 gtacctgttt tccaactcgc tggcaccggc cattgttgcc gcatccatca aagtactgga
    17581 gatggtcgaa gcgggtagcg agctgcgtga ccgtctgtgg gcgaacgcgc gtcagttccg
    17641 tgagcaaatg tcggcggcgg gctttaccct ggcgggagcc gatcacgcca ttattccggt
    17701 catgcttggt gatgcggtag tggcgcagaa atttgcccgt gagctgcaaa aagagggcat
    17761 ttacgtcacc ggtttcttct atccggtcgt tccgaaaggt caggcgcgta ttcgtaccca
    17821 gatgtctgcg gcgcataccc ctgagcaaat tacgcgtgca gtagaagcat tcacgcgtat
    17881 tggtaaacaa ctgggcgtta ttgcctgagg atgtgagatg aaagcgttat ccaaactgaa
    17941 agcggaagag ggcatctgga tgaccgacgt tcctgtaccg gaactcgggc ataacgatct
    18001 gctgattaaa atccgtaaaa cagccatctg cgggactgac gttcacatct ataactggga
    18061 tgagtggtcg caaaaaacca tcccggtgcc gatggtcgtg ggccatgaat atgtcggtga
    18121 agtggtaggt attggtcagg aagtgaaggg cttcaagatt ggcgatcgcg tttctggcga
    18181 aggccatatc acctgcggtc actgtcgcaa ctgtcgtggc ggtcgtaccc atctgtgccg
    18241 caacacgatc ggcgtcggcg ttaaccgccc gggctgcttc gccgaatatc tggtgattcc
    18301 ggcgttcaac gccttcaaaa ttcccgacaa tatctccgac gacctggctt ccatttttga
    18361 tcccttcggt aacgccgtgc ataccgcgct gtcgttcgat ctggttggcg aggatgtgct
    18421 ggtctctggt gcaggcccga ttggtattat ggcagcggcg gtggcgaaac acgttggtgc
    18481 acgcaatgtg gtgatcactg atgttaacga atatcgtctc gaactggcgc gcaaaatggg
    18541 tatcacccgt gcggtcaacg tcgctaaaga aaaccttaat gatgtgatgg cagaactggg
    18601 catggccgaa ggctttgata tcggtctgga aatgtccggt gcgccgccag cgtttcgtac
    18661 catgctcgac accatgaacc acggcggtcg cattgcgatg ctgggtattc caccgtccga
    18721 tatgtctatc gactggacca aagtaatctt taaaggcttg ttcattaaag gtatttacgg
    18781 tcgtgagatg tttgaaacct ggtacaagat ggcggcgctg attcagtcag gtctggatct
    18841 ctcgccgatc attacccatc gtttctctat cgatgatttc cagaagggct ttgacgctat
    18901 gcgttcgggc cagtccggga aagtaattct gagctgggat taacacgaac aagggctggt
    18961 attccagccc ttttgcctga ggataatccg ttaaatatgt aaaatcctgt cagtgtaata
    19021 aagatatcgt aattgcgctg atctcttata tagctgctct cattatctct ctaccctgaa
    19081 gtgactctct cacctgtaaa aataatatct cacaggctta atagtttctt aatacaaagc
    19141 ctgtaaaacg tcaggataac ttcagaggtc gtcggtaatt tatgatgaac agcaccaata
    19201 aacttagtgt tattattccg ttatataatg cgggcgatga tttccgcact tgtatggaat
    19261 ctttaattac gcaaacctgg actgctctgg aaatcattat tattaacgat ggttcaacgg
    19321 ataattctgt tgaaatagca aagcattacg cagaaaacta tccgcacgtt cgtttgttgc
    19381 atcaggcgaa tgctggcgca tcggtggcgc gtaatcgtgg gattgaagtg gcaacgggca
    19441 aatatgtcgc ttttgtcgat gctgacgatg aagtctatcc caccatgtac gaaacgctga
    19501 tgaccatggc gttagaggac gacctcgacg tggcgcagtg caacgctgac tggtgttttc
    19561 gtgaaacggg agaaacctgg aaatccatcc ccagcgatcg ccttcgctca accggcgtat
    19621 taaccggccc ggactggctg cggatggggc tttcttcgcg ccgttggact cacgttgtct
    19681 ggatgggggt ttatcgccgc gatgttattg ttaaaaataa cattaaattt attgccggat
    19741 tacatcatca ggatattgtc tggacaacag aatttatgtt taatgcgctg cgtgcgcgat
    19801 ataccgagca atcgttatat aaatattatc tgcataatac gtcagtgagt cggttgcata
    19861 gacaagggaa caaaaacctt aattatcaac gtcactatat taagattact cgtctgctag
    19921 agaaattaaa tcgaaattat gccgacaaaa ttacgattta tccggaattt catcagcaaa
    19981 taacttacga agcattgcgt gtttgccatg cggtgcgcaa agagccggat attattaccc
    20041 gccaacggat gattgccgag atatttactt ccggtat
//
LOCUS       DAETQN010000078        19739 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.078, whole genome shotgun sequence.
ACCESSION   DAETQN010000078 DAETQN010000000
VERSION     DAETQN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.078"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..510
                     /gene="csgD"
                     /locus_tag="K0W68_002766"
     CDS             <1..510
                     /gene="csgD"
                     /locus_tag="K0W68_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBI3074284.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
     gene            515..904
                     /gene="csgE"
                     /locus_tag="K0W68_002767"
     CDS             515..904
                     /gene="csgE"
                     /locus_tag="K0W68_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBI3074285.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            929..1345
                     /gene="csgF"
                     /locus_tag="K0W68_002768"
     CDS             929..1345
                     /gene="csgF"
                     /locus_tag="K0W68_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBI3074286.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1372..2205
                     /gene="csgG"
                     /locus_tag="K0W68_002769"
     CDS             1372..2205
                     /gene="csgG"
                     /locus_tag="K0W68_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HBI3074287.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2270..2761)
                     /locus_tag="K0W68_002770"
     CDS             complement(2270..2761)
                     /locus_tag="K0W68_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBI3074288.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="K0W68_002771"
     CDS             complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="K0W68_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBI3074289.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="K0W68_002772"
     CDS             complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="K0W68_002772"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBI3074290.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="K0W68_002773"
     CDS             complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="K0W68_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HBI3074291.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5405..5492
                     /locus_tag="K0W68_002774"
     tRNA            5405..5492
                     /locus_tag="K0W68_002774"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5439..5441,aa:Ser,seq:gga)
     gene            5537..5673
                     /locus_tag="K0W68_002775"
     ncRNA           5537..5673
                     /ncRNA_class="other"
                     /locus_tag="K0W68_002775"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5764..6450)
                     /locus_tag="K0W68_002776"
     CDS             complement(5764..6450)
                     /locus_tag="K0W68_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBI3074292.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHEWADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="K0W68_002777"
     CDS             complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="K0W68_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBI3074293.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="K0W68_002778"
     CDS             complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="K0W68_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBI3074294.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="K0W68_002779"
     CDS             complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="K0W68_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBI3074295.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10902..11732)
                     /locus_tag="K0W68_002780"
     CDS             complement(10902..11732)
                     /locus_tag="K0W68_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBI3074296.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(12398..13906)
                     /gene="putP"
                     /locus_tag="K0W68_002781"
     CDS             complement(12398..13906)
                     /gene="putP"
                     /locus_tag="K0W68_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HBI3074297.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            14328..18290
                     /gene="putA"
                     /locus_tag="K0W68_002782"
     CDS             14328..18290
                     /gene="putA"
                     /locus_tag="K0W68_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HBI3074298.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(18330..18968)
                     /gene="rutR"
                     /locus_tag="K0W68_002783"
     CDS             complement(18330..18968)
                     /gene="rutR"
                     /locus_tag="K0W68_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBI3074299.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            19256..>19739
                     /locus_tag="K0W68_002784"
     CDS             19256..>19739
                     /locus_tag="K0W68_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBI3074300.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         5013 a         5256 c         4806 g         4664 t
ORIGIN      
        1 gacgatatat cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
       61 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      121 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      181 ttttatgcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
      241 gaatgctact ttacgcaaaa gcttgccagc tacttgatta cgcattcagg taactatcgt
      301 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataaactg
      361 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
      421 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
      481 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
      541 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
      601 attgctaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
      661 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
      721 gggaagctgg atcactataa cggtcaatca ggacgttatt ttccagactt ttttatttcc
      781 gttgaaaaga gacttcgaga aaactgtcgt ctttgcactg attcaaactg aagaagcact
      841 aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt
      901 ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
      961 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
     1021 tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc aaaactctta
     1081 taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt
     1141 tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa
     1201 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
     1261 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
     1321 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
     1381 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
     1441 gccgccagac cgacattaat gcctcgagct cagagctaca aagatttgac ccatctgcca
     1501 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
     1561 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg
     1621 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgtcagag tttacaaaac
     1681 ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa acggcacagt tgccattaat
     1741 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatca tggttgaagg ttcgattatc
     1801 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     1861 gacacgcaat accagctcga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc
     1921 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     1981 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     2041 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     2101 aatgatggta tcgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaatgac
     2161 attctggtta aataccgcca tatgtcggtt ccaccagaat cctgacagat aaaaatgccg
     2221 gaattaataa tccggcattt tactgtttaa gcgaaagcgt gagtgcccct cacgctttat
     2281 ttttgatttc ctgctcacgg tgagccgtct ttgcactacg cgccgccagc cacaggccgc
     2341 tgtttttcat tgcgtaacca aataccagcc caagcgccag cgaaggtaac accagtttcc
     2401 agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga acaaatgaaa
     2461 gcagcagctg tttggcctga atacacatca gaaacgccac aataccggtt atgacataac
     2521 cgagaatttc cagatgtggt gccagtgcac taccgtaaat aatgaccatc gcccacacca
     2581 cgccactaag cagggttgca gcggagatcg ccagcccttt cagcccacct tgcgggcagg
     2641 caaagtaggc cgtacagccc aagaagcccg cccagcttag taagcccagg gaaacagcca
     2701 cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga agtatgttca
     2761 tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca ttttatgcaa
     2821 caacaaatta tctgttgcag ttatgagtga cgatgatcac atttactctt cagaatcttc
     2881 ttccagctcg tcccacattg cactaatggc atcacgggtt agcggtgcca tggtgcgcca
     2941 gaaaggcgtg gttgcatggg cctccacttt gccgaggaac gcaccacacc agggtaacag
     3001 atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact gatcttccag
     3061 ccaggaagcc gcaagcagca atgtgccgat gtgatcggct ggcgtatccg ccagtggcat
     3121 ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt acgtcctcaa cccatgcgct
     3181 acgatatggc ggcactgcac attcatcgcc gataaacaac gcattgtaat cggcagagac
     3241 ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac tcatcctgct ccagtggcca
     3301 gttcgcagcc agtttcccct cacgaatcag ggtaaacagc ggcaccagta aaggatcttg
     3361 cggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact cgttcattaa
     3421 tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc cataccgcga
     3481 gattcaagga agttcagtaa gcggcgcgga gaaacattca aaatgcgctc tggcggaaaa
     3541 tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa cgcggtgtga
     3601 gaatccgagc ctaatgccac ccaacctcca gcatcacgta ccgctgcggc taccgcacga
     3661 cagttgtctt cactgccctt acgtgagtgt aaaaatgaag aattattgat ttccagcgcc
     3721 acctgatgtt tcgcggctgc ttcagcaacg gctttcacat ctatttcata tttgggattt
     3781 ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat cgcttgtgta
     3841 ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa aaccggcaat aatcagatcc
     3901 agcgagtcaa acattttacc gctgcaatca atttcaccat caacattttt aatgttagct
     3961 tcgatgccgc gcaggatccc taccccatca accactcgcg gccagatacg catgttaatg
     4021 aagtgccagt gatgcggagc atcttccata tccgggccat gatcggtgat cgcaaaaagt
     4081 ttaatgccct tttgtttggc ctgggcaatg taatcactca atgtgctata tgcatgtgtg
     4141 ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg tattcaattc
     4201 ccgccaagga tagcaggaat cctgatgctt tattagtagc cgcgtgcgcg gtcgacttgc
     4261 ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc gagaaatgta ctccacagct
     4321 tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag
     4381 agcggacttt caggcggtaa gggttcacga ttaaaaacat ccaacattgc gcctttaact
     4441 ttgccgctat ccagcgccgc gagcaggtca tcttccacaa catgaacacc acgcgccagg
     4501 ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat aatgccgacg
     4561 gtttcagggg tattcggtaa caaattaatc aatacccgac attggctcag aaatgcagac
     4621 agttcttccc gtccggcaaa gctttgcacg ccaggccacg atttacgggt tcgactccag
     4681 caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa ctttactgcc cagtacgcct
     4741 gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc
     4801 gaactatttt gctggatgcg ataatcgtca aaacgtcgaa accaatgcag cacctgactg
     4861 acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca
     4921 gaagggttca gcatttcagg gtgtgcctgt agcttgctca aaatagaatc aacaccggcc
     4981 ccgagtgcga acaccgcttt aagatcgcgc cctgccagca tttcaacagg aggatgccag
     5041 actaaagcat aatcagcaga gtcattatct ccgcttttcc atgctctgac tcttgcctga
     5101 ggaatagctt tgcgcagtgc ctcaatccac cattgggtat cgaacgttgg gtgataaaag
     5161 atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat ttattcgcac ttatcgttat
     5221 gatctacaaa ggccaccagc ataacaaatc cgtggtcggt ggcaaaaaaa gcagatttcg
     5281 cttattaaaa ccacacattg attgaagttt gaataaacgc gcgatttttt caaaaagttt
     5341 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     5401 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg
     5461 tatcgggggt tcgaatcccc ccctcaccgc catatttaaa gaagagctcg tacgaaagta
     5521 cgggcttttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccggggttcg
     5581 acaactggcg acagccagtt ggacagaccg tgaacgcagt gagcgggctg cccgcagggc
     5641 gagcgaagcg agtcaatccc cccctcaccg ccatctttaa agaagagctc gtacgaaggt
     5701 acgggctttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccgggatact
     5761 tagttaatgc accggttcac tctttgtcgc cgcgactaat gggcgaattt tgtcacgagt
     5821 ctcccagacc ggaggaattt cctccaattt agtgacagaa gctcgtacaa aaggcacccg
     5881 tgcaagagtc ataattgtat ccaaccaacg gtcacgaata atgcgatccg cccattcatg
     5941 actcgggtca tccagctcaa taacacagac tattttattg ttcttctttg aaaccagaac
     6001 ataatccatt gccatgccat tataaattgc aaagaaacgc tttctttctt cttcgctaaa
     6061 ctcaggttgt tctggaaagt tgatcaagcg ccccagagag acctggcaat ggatatgaca
     6121 ataatgtcca aaccattttt ccagattatc gaaatacatt ttttcacaac gcgtgattaa
     6181 ccactctttt ctttccaata aaataccttc ggcaaccatt ccctgaaata actcatctcg
     6241 ctcttgccaa cgagagtcac gataggtttt ctgcggtata tagctccttg tcgacgcctt
     6301 agcggcaggg gaagttgcag atgaagggac gggttgcgat ttagcgatga ttaccgatga
     6361 ggcaactttt cggagtgtca attttctggc aacagcaaaa ccggcgataa atgcaattgt
     6421 caaacaagtt acaaaagcca taaagctcat aattaatttt cacctatcca tgttgattat
     6481 atttcattac caatgtaact cgctgcagcg tgttacatgc caacaatgca acgatcaata
     6541 tatagttagt atcatgtttt gattattaca accatcccta tttagccatt gcggcttgcc
     6601 ttccacaaaa aaataacaac acacaatatt agcaaagaat taattaagat taaccttaca
     6661 tttataatat tatccctaaa gaataaatca gaggaatata ttcggtgtta aacagtcagt
     6721 caaagtatag cccattgttg attaaacctc ataagcaaaa ttggcaccag atctcctgta
     6781 caggttgaat ggccgaaaaa agataggcag atgctcattt ttttggcttc tggaagaaaa
     6841 actctccgcg ctggcagtat tacactggtc tgatgctatg acgaaaaatg cctggtaaga
     6901 gtatctaaac cagactccag ttcagcttat caacacgctc aaagtcgccc ccctgaacgc
     6961 taacctgaag tgaaaatctg ttgaaataaa acaaagcccg gttcgcccgg gctctgcacc
     7021 gataacacac ttaactgtag gcatgcagcg tacgttggca aagtgccgaa cgtacgcagt
     7081 cctctttacc gaaccggacg atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt
     7141 cacttaatcc ggagcacacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga
     7201 taaccgtcac gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat
     7261 tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga
     7321 acggcgcaat ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc
     7381 ctaagcgccg gaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga
     7441 tatctccagg taagaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa
     7501 tcctgtcgac atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaagttt
     7561 ttccgccccc ggcttcgccc gtggcgaata tcagctgctt actctcaata gccttcagat
     7621 agtgcaattg cgcttcattt cgcgcgagga tgggcgaagt atcgcgactg tcgcgggcca
     7681 taccaatggc ttctacgccg cccatctgca caagcgaggt gaccgattct tcttcacgct
     7741 gcttatggct gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca
     7801 ctgctttttg tcttcccata gagagcacct tgagttgttt gtattcatca cacgcgccgt
     7861 tggcagcgcg attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg
     7921 cttttggata aaatgccgaa aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg
     7981 aaaagaaagg atgaggttga aaatgcaaag tgacgagatg actaccggag gagaaaactc
     8041 cgcgagtggt ggcgcgttga ttatctaaaa catgtccagt acaggacgtt accatccgcg
     8101 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     8161 agatctcgca tttattgcaa caatatattt acttatattt aactataaaa cactatttca
     8221 gtgacattag tttctactgg aaagatgaca gagtgatgac agtgatgaaa aaagctgtgt
     8281 gctttcagca ggatttgcat caggcatcag tgcacgatcg ccggatgcgg cgtgaacgcc
     8341 ttatccagcc taccaaaccc gcactgattg taggcatgat aagacgcgca agcgtcgcat
     8401 caggcatctg cgcacaaatg ccttatccgc ctaaaaacat taaacccgca ataacgcgct
     8461 tccgagataa tcgttcgcgt ccttcactcc cggcagcgaa aaaaaataac cgccgccgat
     8521 aggtttaacg tattcctcca gcgcttcgcc attgagcctt ttttgtactg tcaggaagcc
     8581 tttttccaga tcgtgttggt agcagacaaa cagcaacccc atatccagtt gtccggagtt
     8641 ggtgaccccc aacgaataac tgtagccgcg acgcagcatc aggctggact cactctccgc
     8701 cgtgcgggga ttcgccagcc ggatatggct gtccagcgca atcaccttcc cttccgggtc
     8761 gctggcgtaa tcaggcacat catgctcatg ctgcattccc agcggcgcac cggtttgctt
     8821 atcacggcca aaaatcgtct gctgttcttt cagcggcgtt ctgtcccaaa attccactcg
     8881 aaactgaatc aagcgtactg cctgatagct gccaccgatt gtccacgcag gctcctgctg
     8941 atctgccgtt acccacacca ctttttgcat caacttatca ttctggctat cgggattggc
     9001 agtgccgtct ttgaaaccca gcaaattaat cggcgtctct ttgcctttac tacgcgacac
     9061 gtgatcggaa ataaaccctt cccgcttcca gcgcacactg agcaaatccg gcgtgtgttt
     9121 gatgatatcg cgcagcgcat ggataaccgt gtcctgggtg ttggcgcaaa tctgtagcaa
     9181 cacatcacca tgacataacg ccgcatccag cgagtcgttg gggaaacgcg tcatcttctg
     9241 cagctttttt ggcatctgtg gcgcaaggcc aaagcgctca tcaaacaatg agtgacccac
     9301 cgataacgtg atggtgagat tatcgggcgc aatgtagccg ccaagaatgc cggaatcgag
     9361 tggtggcagg cgcggatttg gcgtttctgg tgctgctccg ccctgagtca gaaaagcaaa
     9421 acgctgagtc aacaagcgaa acaaccgctc aagatcggct ttatcgctgg caagcacatc
     9481 aaacgccacc agcatcattg cggcctgttg tggcgtcagg atccctgcct gatgctcacc
     9541 ataaaacggc tgtttctcat tgcgtgcatc cggtgaaagc gtgcccggtg cactttgcgt
     9601 tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc agcgcaccaa tccctttcag
     9661 taaacgtcgg cgtgacggtt cattcacgcc gttttcatct ttatactgca taacgcttaa
     9721 tccagtccca gcacaccgcg aagttgcgcc agatcttccg ccagcgcagt aatcggtcct
     9781 ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta
     9841 cggtatttcg ccagaatggt atcgaccttt ttaaagttgg catcgacttt tgccagcagt
     9901 tccgggttgg ctttttgcag ttgtggacgc agcaaatcga caattttctg cgagccttca
     9961 acgttagcct ggaaatccca cagatcggtg tggctgtagc gatcttcttc accgctaatt
    10021 ttgctggctg ccacttcctc aatcagtccg gctgcgccgc cgaccacttt tgaaggtggg
    10081 aaagccagtt cactgatgcg tttttgcaaa tcgaccacat ctgtataaag ctgctcagcg
    10141 tactgatcca tccctttggt ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa
    10201 ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat cttcacgggc gtcaatgctg
    10261 ccatccagat cggagaacag ttcagcaatc ggttcaatgc gctcatagtg ctggcgcgtc
    10321 ggcgcataca gtgctttcgc tttttcgata tcgcctgctt taatcgcgtc ggtaaaggct
    10381 ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat acgctttata tgcagtaatt
    10441 gcaccaccaa gacttaacag cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg
    10501 acgatcaact tccctttcgg gttagtcagc agaccgcagg tcatatcgta ttcgccaggc
    10561 tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga tattttcccg ctcttccacc
    10621 accatcacgc ctttgaggat ctcccactcc agcgccttct ggctatggtt ctgaataatg
    10681 aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt cgcactgctt atccgtcacg
    10741 gtcactttga cctgcggcac atcagcggcg ttagccataa aagcagaaga aaacagcgca
    10801 gccacgctca actgcaatgc gttacggcgg aagttaatgg tcatgacgag actatccctt
    10861 taaagtatgt tgtaactaag caagagtttg cgtcgtattt gttacgcgga gcgagacgct
    10921 gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg ccgggatgag ataaataaac
    10981 cagacggcga cttcgctgac gctcggtgct tcctgatagc caaaaatccc ttccatcagc
    11041 gtgccaaaca gcgagtgagt tgagagcact gcgctcatat cgaaggcgat ttcctgaaag
    11101 tggttccaca atccggcttc atgaaatgcg cgaatggcac cagcagccag ccctgcggcg
    11161 acgaagagaa taaacaggct ggtccattta aaaaatgcac caagattgag gcgaataccg
    11221 ccccagtaga gcaggaagcc tagcaccacg gcagtagcaa gaccgagcat tgcacccagc
    11281 ggcggccaga tcccgacatc ttgttgaaat gccgccagca ggaaaaagac cgactccagc
    11341 ccttcccttg caacggcaaa aaagaccatc atcaccagcg cccagccatg atgatttcca
    11401 cgctgcaatg cgctatcgac tgcctgctcc agttgtactt tgacgttgcg cgacactttg
    11461 cgcatccaga aaaccatcca ggtaaggatc accacggcga tcaccgccac gataccttca
    11521 aacagttcct gttctttttg cggaaattcg ccggtggttt cgttaatgaa gatacccaag
    11581 cccaggcaca acgcagcggc aagcaacacg ccaatccaca tcacgccaat ccatcggcct
    11641 cgctgggtac gcttaagata gctggcaatc aaactgacaa tcagcgcggc ttcaagtcct
    11701 tcgcgcaaca taatgagaaa cggaacaaac atgccaccca cccttaaacg tttttctgag
    11761 tcaatccatg caaagaaaag taaatcgcag tgatagcgat tatcattacg gcaaagaaaa
    11821 atacaagcgg aatgtttata aaatgatgtt ggaatgtaaa caaagagagg taataccagg
    11881 ggatataaac gattattcca ggtacaaaga tgggattgtt tacaacgagc catgtgttct
    11941 tgccagatgc cgcgtaaacg ccttatccag ccgacaaaac tgtccagact caatgcatca
    12001 cagcttccat gtaaaactga taagcgggtg tatcaagcaa tttcgtccat ccgcgattat
    12061 caaatctaac tttttgcccg gaacataata acagcgcggg tgttggtggc gtcaaaatcg
    12121 ctgaaacgtt ccccgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtctta
    12181 caggacgtta cctcgcgccc gacccggtag ccgaagggga taagctgaag gtaccgcgca
    12241 gcggcgattt tgttcgccag agcccggggg tgcagggggc ggcggcgact ggccgccccc
    12301 tgcgcgctcc ctgcaccatt caatttatat ggcgaggcac attaagggag cgcaacccgc
    12361 tgctgaagcc aaacaaaacc gccgcaggct aagtccctta gttttcctgc aaccgtgacg
    12421 gcggagccga atgatagtgc gcatcggcct cggcaaagcg tttttgcatc gccgctgacg
    12481 gcgctttacc cagcaaacta aacactacaa tcccgatact gccgaagatg aagcccggaa
    12541 taatttcgta cagtcccagc cagccgaact gcttccagac gataactgtc agcgcaccga
    12601 tgatcatccc cgccagcgca ccgttgcgcg tcatgcgtga ccacatcacc gagaacagca
    12661 ccaccggacc aaacgccgcg ccaaagcctg cccacgcgta gctcactaag cccagcacgc
    12721 ggttttccgg gtttgccgcc agcgcaatcg ccaccagcgc caccaccagc accatcacac
    12781 gtcctaccca caccagctct ttctggctgg cctgtttacg cagaaacgct ttgtataaat
    12841 cttcggtaat cgcactggag cacaccagca gctggcaact taaggttgac attaccgccg
    12901 ccagaatcgc cgacagcaga atcccggcaa tccacgggtt aaacagaatt tgcgccagtt
    12961 cgataaacac gcgttcagcg ttctggttta ccgcacctgc taccgccgga tgctcgttaa
    13021 agtaagcgat accaaagaag ccaacagcca ccgcccctgc caggcaaagg atcatccagg
    13081 tcatactgat acgacgcgca tggacaatgc tgtggtgaga atctgccgcc ataaaacgcg
    13141 ccaggatgtg cggctgcccg aagtaaccca gcccccaacc catcaatgag ataatggcaa
    13201 caaagttcag ccctttgagc atatccacgt tttcgatgct cttttgtttg atcacttcca
    13261 gcgagtcacc aaagccaccg acactgataa tgacgataac cggcgtcagg atcagggcaa
    13321 aaatcatcag gctggcctgt acagtgtcag tccagctcac cgcgaggaaa ccgccaataa
    13381 aggtgtaaag gatcgtcgac gcagctcccg ccaacagagc cgtttcgtaa ctcatgccaa
    13441 aggtactttc aaacagacgc gcgcctgcca caatgcccga agcgcaataa atggtgaaga
    13501 acagcaaaat aaccagcgcg gagataatgc gcaaaatgcg gcttttatct tcaaaacgcc
    13561 cggtgaaata atccggcaat gttaaggcgt tattattgta ttcggtatgc acacgcaacc
    13621 gcccggccac cagcttccag ttgatccacg cgcctaatgt caggccaatg gcgatccagc
    13681 tttcggaaat cccggaaaga aaaaccgcgc caggcaaccc cattaacagc cagccgctca
    13741 tatccgacgc acccgccgat aatgccgtca cgaatggccc aagactacga ccgcccagaa
    13801 tatagtcgtc aaagtttttc gttgatcgcc aggcgataaa cccaatcaat atcatgccaa
    13861 agatatagac acaaaatgtc accaacatcg gtgtgctaat agccatctaa agtctccaaa
    13921 aaattattat cggcaatgtc gaaacttgcc gttatatctg ccaccggaac ggggtaacag
    13981 agtttatgtt ttaccagggc gaccgtatcc tgccggaagc gctggttatt cacaatcgat
    14041 ttaacacacc atttacatta aattttagtg ctcagcgaca ctatttttca tcaggttgca
    14101 ctctctcaca ttttttgcgg ttgcaccttt caaaaaggtt aactgccgca gagaaaaagt
    14161 ctgagttatt ttttaatcac tgtcatatcg atttctttta ttaacatttc attcattttt
    14221 aagcttgcta cgcaagtcac atttaacatg gttgcacaaa gttgcaacat catggatatt
    14281 tcacgataac gttaagttgc acctttcaga acaacaggag taatggcatg ggaaccacca
    14341 ccatgggggt taagctggac gacgcgacgc gtgagcgtat taagtctgcc gcgacacgta
    14401 tcgatcgcac accacactgg ttaattaagc aggcgatttt ttcttatctc gaacaactgg
    14461 aaaacagcga tactctgccg gagctacctg cgctgctttc tggcgcggcc aatgagagcg
    14521 atgaagcacc gactccggca gaggaaccac accagccatt cctcgacttt gccgagcaaa
    14581 tattgcccca gtcggtttcc cgcgccgcga tcaccgcggc ctatcgccgc ccggaaaccg
    14641 aagcggtttc tatgctgctg gaacaagccc gcctgccgca gccagttgct gaacaggcgc
    14701 acaaactggc gtatcaactg gccgataaac tgcgtaatca aaaaaatgcc agtggtcgtg
    14761 caggaatggt ccaggggtta ttgcaggagt tttcgctgtc atcgcaggaa ggcgtggcgc
    14821 tgatgtgtct ggcagaagcg ttgttgcgta ttcccgacaa agccacccgc gacgcgttaa
    14881 ttcgcgacaa aatcagcaac ggtaactggc agtcacacat tggtcgtagc ccgtcactgt
    14941 ttgttaatgc cgctacctgg gggctgctgt ttactggcaa actggtttcc acccataacg
    15001 aagccagcct ctcccgctcg ctgaaccgca ttatcggtaa aagcggtgaa ccgctgatcc
    15061 gcaaaggtgt ggatatggcg atgcgcctga tgggcgagca gttcgtcacg ggtgaaacca
    15121 tcgcggaagc gttagccaat gcccgcaagc tggaagagaa aggtttccgt tactcttacg
    15181 atatgctggg cgaagctgca ctgactgccg cagacgcaca ggcgtatatg gtttcctatc
    15241 agcaggcgat tcacgccatt ggtaaagcgt ctaacggtcg tggcatctat gaaggtccgg
    15301 gcatttcaat caaactgtcg gcgctgcatc cgcgctacag ccgcgcccag tatgaccggg
    15361 taatggaaga gctttacccg cgtctgaaat cactcaccct gctggcgcgt cagtatgata
    15421 ttggtattaa catcgatgcc gaagaggccg atcgtctgga gatctccctc gatctgctgg
    15481 aaaatctctg tttcgagccg gaactggcag gctggaacgg catcggtttt gttattcagg
    15541 cttatcaaaa acgctgcccg ttggtgatcg attacctggt tgatctcgcc acccgcagcc
    15601 gtcgccgtct gatgattcgc ctggtgaaag gcgcgtactg ggatagtgaa atcaagcgtg
    15661 cgcagatgga cggccttgaa ggctatccgg tttatacccg caaggtgtat accgacgttt
    15721 cttatctcgc ctgtgcgaaa aagctgctgg cggtgccgaa tctaatctac ccgcagttcg
    15781 cgacgcacaa cgcccatacg ctggcagcga tttatcaact ggcaggtcag aactactatc
    15841 cgggtcagta cgagttccag tgcctgcatg gtatgggcga gccactgtac gagcaggtca
    15901 ccgggaaagt tgccgacggt aaacttaacc gaccgtgtcg tatttatgcc ccggttggca
    15961 cacatgaaac gctgctggcg tatctggtgc gtcgcctgct ggaaaacggt gctaacacct
    16021 cgtttgttaa ccgtattgcc gacacctctt tgccactgga tgaactggtc gccgatccgg
    16081 tcactgctgt agaaaaactg gcgcaacagg aagggcaaac tggattaccg catccgaaaa
    16141 ttcccctgcc gcgcgatctt tacggtcacg ggcgcgacaa ctcggcaggg ctggatctcg
    16201 ctaacgaaca ccgtctggcc tcgctctcct ctgccctgct caatagtgca ctgcaaaaat
    16261 ggcaggcctt gccaatgctg gaacaaccgg tagcggcagg tgagatgtcg cccgttatta
    16321 accctgcgga accgaaagat attgtgggct ttgtgcgtga agccacgcca cgtgaagtag
    16381 aacaggcgct ggaaagtgcg gttaataacg cgccaatctg gtttgccacg cctccggctg
    16441 aacgcgcggc gattttgcat cgcgctgccg tgctaatgga aagccagatg cagcaactga
    16501 ttggtattct ggtgcgtgaa gccggaaaaa ccttcagtaa cgccattgcc gaagtgcgcg
    16561 aagcggtcga ttttctccac tactacgccg gacaggtgcg ggatgatttc gctaacgaaa
    16621 cccaccgtcc attagggcct gtggtgtgta tcagtccgtg gaacttcccg ctggctattt
    16681 tcaccgggca gatcgctgcc gcactggcgg caggtaacag cgtgctggca aaaccggcag
    16741 aacaaacgcc gctgattgcc gcgcaaggga tcgccatttt gctggaagcg ggtgtacccc
    16801 ctggcgtggt gcaattgctg ccaggtcagg gggaaaccgt gggcgcgcaa ctgacgggtg
    16861 atgatcgcgt gcgcggggtg atgtttaccg gttcaaccga agtcgctacg ttactgcagc
    16921 gcaatatcgc cagccgcctg gacgctcagg gtcgccctat tccgctcatc gctgaaaccg
    16981 gcggcatgaa cgcgatgatt gtcgattctt cagcactgac cgaacaagtc gtcgtggatg
    17041 tgctggcctc ggcgttcgac agtgcgggtc agcgttgttc ggcactgcgc gtgctgtgcc
    17101 tgcaagatga gattgccgac cacacgttga aaatgctgcg cggtgcaatg gccgaatgcc
    17161 ggatgggtaa tccgggtcgc ctgaccaccg atatcggtcc ggtgattgat agcgaagcga
    17221 aagccaatat tgagcgccat attcagacca tgcgtagcaa aggccgtccg gtgttccagg
    17281 cggtgcggga aaacagcgaa gatgcccgtg aatggcaaag cggcaccttt gtcgccccga
    17341 cgctgatcga actggatgac tttgccgaat tgcacaaaga ggtctttggt ccggtgctgc
    17401 atgtggtgcg ttacaaccgt aaccagctac cagagctgat cgagcagatt aacgcttccg
    17461 gctatggtct gacgcttggt gtccatacgc gtattgatga aaccatcgcc caggtcactg
    17521 gctcggccca tgttggtaac ctgtatgtta accgtaatat ggtgggcgca gtggttggtg
    17581 tgcagccgtt cggcggcgaa gggttgtccg gtaccgggcc gaaagcaggc ggtccgctct
    17641 atctctaccg tctgctggcg aatcgcccgg aaagtgcgct ggcagtgacg ctcgcgcgtc
    17701 aggatgcaga gtatccggtc gatgcacagt tgaaagccgc attgactcag ccgctaaatg
    17761 cactgcggga atgggcagca aatcgtccag aattgcaggc gttatgtacg caatatggcg
    17821 agctggcgca ggcaggaaca caacgattgc tgccggggcc gacgggtgaa cgcaacacct
    17881 ggacgctgct gccgcgtgag cgcgtgttgt gtattgccga tgatgagcag gatgcgctga
    17941 ctcagctcgc cgccgtgctg gcggtgggca gccaggtact gtggccggat gacgcgctgc
    18001 atcgtcagct agtaaaggca ttgccatcgg cagtcagcga acgtattcaa ctggcgaaag
    18061 cggaaaatat aaccgctcaa ccctttgatg cggtgatctt ccacggtgat tcggatcagc
    18121 ttcgcgcatt gtgtgaagcc gttgctgcgc gggatggcgc aattgtttcg gtgcagggtt
    18181 ttgcccgtgg cgaaagcaat atccttctgg aacggctgta tatcgaacgt tcgctgagtg
    18241 tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa acaggtagcc
    18301 ggaggatgtt acaacctcct ccggcatctt taacgtggtc gaatcccctc aataataatc
    18361 cgctgcacgt tttcaaccgt ttgattgaaa aatatctcat cgcgcaacgt cgcgcctgtc
    18421 accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca aatcatgaaa
    18481 atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc ggcaatcagc
    18541 gcagatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat taacagcggc
    18601 gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc gcgggagact
    18661 tccagcttca gacggatgta ctcttttatc gccgccagcg gagcgaaatc ttcacgaaac
    18721 gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat atacagcgcc
    18781 tcttttgacg gaaagtaata cagcagattg gtttttgaaa cacccgccaa ctctgcgatc
    18841 tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc tgcgctaaga
    18901 atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt cactgcgcct
    18961 tgcgtcatgc gctctcccct cttgtagatt ggcaacagga tagcaaaacc cccgcccggc
    19021 ctcgaccttc tgcactctca tcgcgcacag atgcatgttt tatgtgcaac tgttttgacc
    19081 gtttagtcca ctttttatca gatattttag ataacgtaat attaacatat taataacatt
    19141 aactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg aaaaggatat
    19201 gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt tgatgatgaa
    19261 aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc acgcgccgca
    19321 gtacatgccg acctttgaac tgaataaagc cattgtgcag aaagcggagc attaccattt
    19381 cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg agttctggga
    19441 tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct cgcgcattca
    19501 gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc gtatggccgc
    19561 aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg gctggcaaaa
    19621 gcccgtgtat gagcagatgg gtatctggcc tggcgatgac tatttctccc gtcgttacga
    19681 ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga aaagcgatt
//
LOCUS       DAETQN010000079        19576 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.079, whole genome shotgun sequence.
ACCESSION   DAETQN010000079 DAETQN010000000
VERSION     DAETQN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19576
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.079"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..110)
                     /locus_tag="K0W68_002785"
     CDS             complement(<1..110)
                     /locus_tag="K0W68_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3074301.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCR"
     gene            168..413
                     /locus_tag="K0W68_002786"
                     /pseudo
     CDS             168..413
                     /locus_tag="K0W68_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
     gene            complement(464..1231)
                     /locus_tag="K0W68_002787"
     CDS             complement(464..1231)
                     /locus_tag="K0W68_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI3074302.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1427..2335
                     /locus_tag="K0W68_002788"
     CDS             1427..2335
                     /locus_tag="K0W68_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBI3074303.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2332..3594
                     /locus_tag="K0W68_002789"
     CDS             2332..3594
                     /locus_tag="K0W68_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBI3074304.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3591..4229
                     /locus_tag="K0W68_002790"
     CDS             3591..4229
                     /locus_tag="K0W68_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBI3074305.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4234..5010
                     /locus_tag="K0W68_002791"
     CDS             4234..5010
                     /locus_tag="K0W68_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBI3074306.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            5099..6463
                     /locus_tag="K0W68_002792"
     CDS             5099..6463
                     /locus_tag="K0W68_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HBI3074307.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALCASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6557..7549)
                     /gene="rpoS"
                     /locus_tag="K0W68_002793"
     CDS             complement(6557..7549)
                     /gene="rpoS"
                     /locus_tag="K0W68_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBI3074308.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7612..8751)
                     /gene="nlpD"
                     /locus_tag="K0W68_002794"
     CDS             complement(7612..8751)
                     /gene="nlpD"
                     /locus_tag="K0W68_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBI3074309.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8891..9517)
                     /gene="pcm"
                     /locus_tag="K0W68_002795"
     CDS             complement(8891..9517)
                     /gene="pcm"
                     /locus_tag="K0W68_002795"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBI3074310.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9511..10272)
                     /gene="surE"
                     /locus_tag="K0W68_002796"
     CDS             complement(9511..10272)
                     /gene="surE"
                     /locus_tag="K0W68_002796"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBI3074311.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10253..11302)
                     /gene="truD"
                     /locus_tag="K0W68_002797"
     CDS             complement(10253..11302)
                     /gene="truD"
                     /locus_tag="K0W68_002797"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBI3074312.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11299..11778)
                     /gene="ispF"
                     /locus_tag="K0W68_002798"
     CDS             complement(11299..11778)
                     /gene="ispF"
                     /locus_tag="K0W68_002798"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBI3074313.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11778..12488)
                     /gene="ispD"
                     /locus_tag="K0W68_002799"
     CDS             complement(11778..12488)
                     /gene="ispD"
                     /locus_tag="K0W68_002799"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBI3074314.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12507..12818)
                     /gene="ftsB"
                     /locus_tag="K0W68_002800"
     CDS             complement(12507..12818)
                     /gene="ftsB"
                     /locus_tag="K0W68_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBI3074315.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(13012..13335)
                     /locus_tag="K0W68_002801"
     CDS             complement(13012..13335)
                     /locus_tag="K0W68_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBI3074316.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13385..13990)
                     /gene="cysC"
                     /locus_tag="K0W68_002802"
     CDS             complement(13385..13990)
                     /gene="cysC"
                     /locus_tag="K0W68_002802"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBI3074317.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13990..15417)
                     /gene="cysN"
                     /locus_tag="K0W68_002803"
     CDS             complement(13990..15417)
                     /gene="cysN"
                     /locus_tag="K0W68_002803"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBI3074318.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15419..16327)
                     /gene="cysD"
                     /locus_tag="K0W68_002804"
     CDS             complement(15419..16327)
                     /gene="cysD"
                     /locus_tag="K0W68_002804"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBI3074319.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16579..17616
                     /gene="iap"
                     /locus_tag="K0W68_002805"
     CDS             16579..17616
                     /gene="iap"
                     /locus_tag="K0W68_002805"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBI3074320.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(17884..18177)
                     /gene="cas2"
                     /locus_tag="K0W68_002806"
     CDS             complement(17884..18177)
                     /gene="cas2"
                     /locus_tag="K0W68_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HBI3074321.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18174..19097)
                     /gene="cas1e"
                     /locus_tag="K0W68_002807"
                     /pseudo
     CDS             complement(18174..19097)
                     /gene="cas1e"
                     /locus_tag="K0W68_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
     gene            complement(19094..>19576)
                     /gene="cas6e"
                     /locus_tag="K0W68_002808"
     CDS             complement(19094..>19576)
                     /gene="cas6e"
                     /locus_tag="K0W68_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HBI3074322.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         4644 a         5276 c         4889 g         4767 t
ORIGIN      
        1 ttacggcagt gagagcagag atagcgctga tgtccggcag tgcttttgcc gttacgcacc
       61 accccgtcag tagctgaaca ggagggacag ctgatagaaa cagaagccac tggagcacct
      121 caaaaacacc atcatacact aaatcagtaa gttggcagca tcaccccatc gcacatgcag
      181 attatccggg tgatgaatat cagtttggta aagaaatagc agaaagcgaa ttactctggc
      241 ctgttgatcg tgtgcagaaa tcgcttaatg gcgagttaca acaaataaac ggcgctgatt
      301 attttatatt tggacatatg atgtttgata acattcagac gttcgctaac cagatttata
      361 ttgataccgg cacgccgaaa agcgggcggc tgtcatttta taaaataaag tagtctcatg
      421 cttcttctgt gaagcatgag taaccaaatg ttattgcagg ccattattca tttttcgcta
      481 ccagcaaaga gagatcctgc ttcgccagcg cgcgactggc actctccggc aaaccgtcgt
      541 ctgtgataat ctgatcaaac tcgcttaatg gtaacgccag ccatgtcgcc acctgaccat
      601 atttcgtcgc atcacagacc aaaactcgct ggcggctggc actggcaatc gcccgtttca
      661 ccgtgacttt atcttccgct ggcgtagaaa tcccccgcac actccatgac gatgcagaaa
      721 taaaagcctg atcaatcatc aggctgcgca gcatggtcgc agcggcttcc ccgacacagg
      781 aacggttttc ccgacacact gcaccgccag tgtgaataat tgtgcaatta ctgttgtcga
      841 gcaagtagtt cgcaataacg aaatcgtttg tgaccacagt cagtgactcc atgtgaatca
      901 gatgctgtgc tatcgctaac gtggtcgttc ccgcatccag atagatacaa cttcccggct
      961 gaacaagact tgccgccagc ttgccaatag ccgctttttg cgtcattgcc agcgcagttt
     1021 ttacctgatg agaaggttca tgcgccacgc gtcccggaga ctggacgcct ccggacacca
     1081 gcacaacggc tccctgctgc tccagttttt gtaaatcccg acgaatggtc atatgtgaca
     1141 cattcattct gtccgttagt tcagcaatac tgacaatgcc tttttcagct accatctcaa
     1201 ggatgatttg gcgacgctct acgggtatca acttttgctc cttcctttgt cctgctgaca
     1261 ttctacgcta tttgcctgcg aaacgtgcgc ggcgcaacta acgcttagtt cacataaaat
     1321 aacacacaat gttaatttat gtgaatcagc tcaccatatc gttatcttcc agcgcttata
     1381 ttcacaatat caaacaaaat atcacttaaa ttaacaatga gagcagatga aaacgggatc
     1441 tgagtttcat gtcggtatcg ttggcttagg gtcaatggga atgggagcag cactgtcatg
     1501 tgtccgcgca ggtctttcta cctggggcgc agacctgaac agcaatgcct gcgctacgtt
     1561 gaaagaggca ggtgcttgcg gggtttctga taacgccgcg acgtttgccg aaaaactgga
     1621 cgctctgctg gtgctggtgg ccaatgcggc ccaggttaaa caggtgctgt ttggtgaaac
     1681 aggcgttgca caacatctga aacccggtac ggcagtaatg gtttcttcca ctatcgctag
     1741 tgctgatgcg caagaaattg ctaccgctct ggctggattc gatctggaaa tgctggatgc
     1801 gccagtttct ggtggtgcag taaaagccgc taacggtgaa atgactgtca tggcctccgg
     1861 tagcgatatt gcctttgaac gactggcacc cgtgctggaa gccgttgccg gaaaagttta
     1921 tcgcataggt gcagaaccgg gactaggttc gaccgtaaaa attattcacc agttgttagc
     1981 gggcgtacat attgctgccg gagccgaagc gatggcactt gcagcccgtg cggggatccc
     2041 gctggatgtg atgtatgacg tcgtgaccaa tgccgccgga aattcctgga tgttcgaaaa
     2101 ccggatgcgt catgtggtgg atggcgatta caccccgcat tcagccgtcg atatttttgt
     2161 taaggatctt ggtctggttg ccgatacagc caaagccctg cacttcccgc tgccattggc
     2221 ctcaacagca ttgaatatgt tcaccagcgc cagtaacgcg ggttacggga aagaagacga
     2281 tagcgcagtt atcaagattt tctctggcat cactctaccg ggagcgaaat catgatcaag
     2341 attggcgtta tcgccgatga ttttaccggc gcgacggata tcgccagttt tctggtggaa
     2401 aacggtctac caacggtaca aattaacggt gttccaacag gtaaaatgcc ggaagcaatc
     2461 gacgcactgg tgatcagcct gaaaacgcgc tcctgtccgg tggttgaagc cacacagcaa
     2521 tcgctggcgg ctctgagctg gttgcaacag caaggttgca aacagatcta tttcaaatac
     2581 tgctctactt tcgacagtac ggcgaaaggt aatattggcc cggttaccga tgccttaatg
     2641 gatgctctcg acacgccgtt tacggtcttc tctccggccc tgtcggtcaa cggacgtacg
     2701 gtttatcagg ggtatttgtt cgtaatgaat caactgctgg ccgaatccgg gatgcgccat
     2761 cacccggtaa atcccatgac cgacagctat cttccccgtc tggttgaagc gcaatccaca
     2821 gggcgctgcg gcgtcgtttc ggcacatgtt ttcgaacaag gcgtggatgc cgttcgtcaa
     2881 gagctggctc gcttacagca agagggctac cgctacgcgg tgcttgatgc gctgaccgaa
     2941 caccatctgg aaattcaggg agaagccttg cgcgatgccc cactggtaac gggcggttct
     3001 ggtctggcga ttggcctggc ccggcagtgg gcgcaagaaa acggtaacca ggctcgcgaa
     3061 gcagggcgtc cgctcgctgg gcgcggcgta gtgctctccg gttcatgctc tcaaatgacc
     3121 aaccgccagg tggcacatta ccgtcaaatt gcaccagccc gtgaagttga tgtggcacgc
     3181 tgcctctcaa ccgaaactct ggccgcttat gcacacgaac tggcagagtg ggttctgggc
     3241 caggaaagtg tacttgctcc actggttttt gccaccgcca gcactgacgc attggcagca
     3301 attcaacagc aatacggtgc acaaaaagcc agtcaggcag tagaaactct gttttctcaa
     3361 ctagcggcgc ggttagcagc ggaaggcgtg acacgcttta ttgtcgcagg cggtgagacc
     3421 tccggcgtag tcacacagag cctgggcata aaagggtttc atattggccc aaccatttcc
     3481 cccggcgtgc cgtgggtaaa cgcactggat aagcctgtct cactcgccct taaatctggc
     3541 aacttcggtg atgaagcctt tttttcacga gcccaaagag agtttttatc atgagcgatt
     3601 tcgcaaaagt agagcagtct ttgcgagagg agatgacgcg gattgccagt tcattctttc
     3661 agcgcggcta tgcaaccggt tcggctggca atctgtcgct gcttttacct gacgggaatt
     3721 tactggcgac accgacaggt tcatgcctgg gcaatctcga tccgcagcgg ctttccaaag
     3781 tcgccgcgga tggcgaatgg ttaagtggtg acaaaccctc gaaagaggtg ctctttcatc
     3841 tggcgctgta tcgcaacaat ccgcgctgta aagcggtggt gcatttgcac agcacatggt
     3901 cgacggcgct ttcctgcctg caagggctgg acagcagcaa cgttattcgt ccgttcacac
     3961 catacgtggt gatgcggatg ggaaatgtcc cgctggtgcc ttattaccga ccgggcgata
     4021 aacgcatcgc acaggatctg gcggaactgg cagcagacaa tcaggctttt ttactggcaa
     4081 atcatggccc agtggtttgc ggtgaaagcc tgcaagaagc cgccaacaat atggaagagc
     4141 tggaggaaac ggcaaagctg atttttattc tcggtgaccg cccgatccgt tatctgaccg
     4201 caggtgaaat tgcggaatta aggagttaat gcaatgcctc gttttgcagc taatttatcc
     4261 atgatgttca ccgaagtgcc ttttattgaa cgcttcgccg cagcgcgaaa agccgggttc
     4321 gatgctgtgg aatttctgtt tccctatgac tactccaccc tgcaaatcca aaagcaactg
     4381 gagcaaaacc atctgacact ggcgctgttc aataccgctc ccggagatat taatgcgggg
     4441 gaatggggat tatccgccct tcccggacgt gagcacgaag cacacgcaga catcgacctg
     4501 gcactggaat atgcattggc gctcaattgt gaacaagtcc atgtgatggc aggcgtcgtg
     4561 cccgctggcg aagacgctga acggtaccgg gcagtattta tcgataatct ccgctacgct
     4621 gccgaccgct ttgcgctaca cggcaagcga atattagttg aagcactcag ccccgacgtt
     4681 aagcctcatt atctcttctc cagccagtat caggcactgg ctattgttga agaggttgcg
     4741 cgagataacg tgtttattca actagatact tttcatgcgc aaaaagtaga tggcaacctg
     4801 acgcatttaa tccgcgacta cgctgggaaa tatgcgcacg tacaaattgc cggactacct
     4861 gatcgacatg aaccggacga tggagaaatc aactatccgt ggctgttccg cctgttcgat
     4921 gaggtgggat atcagggctg gatcggttat gaatataaac ctcgtggcct caccgaagaa
     4981 gggcttggct ggtttgacgc ctggcgctaa ttcgttaaat ccctaattac aacgtaccca
     5041 tacatccccc catctacgga tggggtaggt taactataac tttcagacag ggtttcccat
     5101 gtccacaatt acattgttat gcattgcgtt agctggcgta atcatgctgt tgctgctggt
     5161 catcaaggca aaggtacaac cattcgttgc tctgctcctc gtcagcctgt tagtcgcact
     5221 tgcggcgggt ataccggcgg gcgaagtggg taaagtgatg atcgccggga tgggcggtgt
     5281 gcttggttcc gtcactatta ttattggtct gggcgctatg ctggggcgta tgatcgaaca
     5341 ctctggtggt gcagagtcac tggctaatta tttcagtcgc aagttaggtg acaaacgaac
     5401 tatcgctgcg ctgactctgg cagcgttctt cctcggtatt cccgtcttct ttgatgtcgg
     5461 ctttattatt cttgctccaa tcatttacgg ttttgccaag gttgccaaaa tatcgccact
     5521 caaatttggc ctgcctgtcg ctgggatcat gctaactgta catgtggcgg taccgccgca
     5581 tccaggccct gtcgccgcag cggggttact ccacgcagac atcggctggc taaccatcat
     5641 cggtattgcg atttctattc ccgtagggat tgttggctac tttgcagcga aaataatcaa
     5701 taagcaccaa tatgcgatgt cggtggaagt actggaacag atgcaactgg ctccggccag
     5761 tgaggaaggc gcgacaaaat taagcgataa aataaatcca ccgggcgtcg cgctggtcac
     5821 ctcgctaatt gttattccta tcgcgattat catggcgggt acggtttccg caacactgat
     5881 gccgccttcg catcccctgc ttggtacgct acagctgatc ggctcaccaa tggtagctct
     5941 aatgattgcg ctggtgctgg cattctggtt attggcttta cgtcgcggct ggagcttaca
     6001 acacaccagc gacattatgg gctcagccct tcccactgcg gcggtagtga ttttggttac
     6061 tggtgctgga ggggtatttg gcaaagtgct ggtggaatcg ggcgtcggca aagcccttgc
     6121 caacatgcta caaatgattg acctgcctct gttaccagcc gcatttatta tttcactggc
     6181 gctgtgtgca tcgcaggggt cagcaaccgt agcaatcctg acaaccggcg ggttactctc
     6241 agaagcggtg atgggattga atccgattca gtgcgtattg gtgacgctgg cagcctgctt
     6301 tggtgggctt ggggcgtcac atattaatga ctcagggttc tggattgtga ccaaatatct
     6361 ggggttgtcg gtagcagacg gtctgaaaac ctggacagtg ttaacgacca ttctcggttt
     6421 taccggattc ttaattacct ggtgcgtatg ggcggtaatt tgaccttaat aaaaaggtcc
     6481 gatgagcatc ggacctttta ttgtgcacag aaaaggccag cctcgcttga gactggcctt
     6541 tctgacagat gcttacttac tcgcggaaca gcgcttcgat attcagcccc tgcgtttgca
     6601 ggatttcacg caaacggcgc aggccttcaa cctgaatctg gcgaacacgt tcacgggtga
     6661 ggccaatttc acgacctaca tcttccagtg ttgccgcttc gtaccccagc aaaccgaatc
     6721 gacgtgccag tacttcacgc tgtttggcgt tcagctcgaa cagccatttg acgatgctct
     6781 gcttcatatc gtcatcttgc gtggtatctt ccggaccgtt ctctttttca tcggccagga
     6841 tgtccagcaa cgctttttcg gaatcaccac ccagcggggt gtctaccgag gtaatgcgct
     6901 cgttaagacg aagcatacgg ctgacgtcat caactggctt atccagttgc tctgcgatct
     6961 cttccgcact tggttcatgg tccagcttat gggacaactc acgtgcggtt cgcaggtaaa
     7021 cgttcagctc ctttacgatg tgaatcggca aacgaatagt acgggtttgg ttcataatcg
     7081 cccgttcaat cgtctggcga atccaccaag ttgcgtatgt tgagaagcgg aaaccacgtt
     7141 ccgggtcaaa cttctctacc gcgcggatca gccccaggtt gccctcttcg ataaggtcca
     7201 gcaacgccag accacgattg ccataacggc gggcaatttt taccaccaga cgcaagttac
     7261 tctcgatcat ccggcggcga gaggcgacat ctccacgcag tgcgcgacgc gcaaaataaa
     7321 cttcttcttc ggccgttaac agtggtgaat aaccaatctc accaaggtaa agctgagtcg
     7381 cgtccaacac acgctgtgtg gctccctgcg ataacagttc ctcttcggcc aaatcgttat
     7441 cactgggttc ctcttctact aaggcctttt cgtcaaaaac ctcaactccg ttctcatcaa
     7501 attccgcatc ttcatttaaa tcatgaactt tcagcgtatt ctgactcata aggtggctcc
     7561 tacccgtgat cccttgacgg aacattcaag caaaagcctg gttccgccga tttatcgctg
     7621 cggcaaataa cgcagcgggt ttacggattt ccccttgtaa cgaatttcaa aatgcaagcg
     7681 tgttgaactg gttccggtgc tacccatggt tgctattttt tgccccgcct tcacttcttg
     7741 ttgttcccgg accagcattg tgtcgttatg ggcgtaggca ctcaggtaat catcattatg
     7801 tttgatgata atcagattac cgtagccgcg cagcgcgtta ccggcataaa caacgcggcc
     7861 atctgcggtc gcgataattg cctgtccttt gctgcctgcg atatcaatcc ccttgttgcc
     7921 cccctcagaa gcgccaaagg tttcgatcac tttgccctca gtcggccagc gccaggtgga
     7981 gataggcgta ctggttgatg tactgctgac agtcggctcg gttgtgcttg ctgttggtac
     8041 cgttacaggc gctgtgactg tggtcgcagt tggcttgttg ttcggcaaca ttttgttagc
     8101 actctgttca cccgaagact cagaatacgt aattgtcggt tgcgacgcaa cagcaacggt
     8161 ggaattttgt gcaggcttga tcacaactcc ttgctctgct gcgtcggcct gggtaatggc
     8221 atttccgcca gtgattggcg taccggaagc attacccacc tgcaaggtct gaccaacgtt
     8281 cagcgcgtat ggtgcctgaa tattgttgcg ctgagcaagg tcacggaaat cgttgccagt
     8341 aatccaggcg atatagaaaa gtgtgtcgcc ttttttcacg gtataggtac tgccgctata
     8401 actgcctttc ggaatgttcc catactgacg gttatagacg atgcgtccgt tttccatctg
     8461 taccggctgc tgagctactg gctgcactgg ctggatttgc ggttgttgag tagcctgaat
     8521 ttgtggctgc tgtaccggct gaatttgcgg ttgctgggct gtagacgtcg tccccatttt
     8581 cggcggcggc gtaatcaaca taccagaatt agtatttgca ggcgcattgc cattaacgga
     8641 gctgaccggt gccggtggat ttgaagtgtc agaacagcct gccagccata gcgaaaccag
     8701 tgacaaagca gcaatgcggc gaacggtgaa ttttgggctt cccgcgctca tttatccccc
     8761 aggaaaaatt ggttaataac cagtgacata attaccgtgc aaggcaccct actgaacact
     8821 ggaaaagatg ttcacgatac gctgacctgc ggcaaaataa ccaggaaaaa tccaggtatt
     8881 tcctcacgtt ttaagccagc tcacccttca ctaaagggac aaagcgcacg gcctccacgg
     8941 tatcgataat aaattcgcct ccccgacgac gcacccgttt caaatactgg tgctcctccc
     9001 ctacgggtaa gacgagaatc ccgccttcgt ccagctgcgt cattagcgca gttggaattt
     9061 ccggcggtgc cgccgtaaca atgatagcgt caaacggcgc acgtgcctgc caaccttgcc
     9121 atccatcgcc atgacgggtt gaaacattat gtaaatcaag atttttcagg cggcgacgtg
     9181 cctgccactg caagccttta atccgttcaa ccgagcaaac atgctggaca agatgcgcta
     9241 ggattgccgt ttgatatccc gaaccagtgc caatttccag cacccgcgac tgcggcgtca
     9301 gctcgagtaa ttcggtcatt cgcgccacca tatacggctg cgaaattgtc tgcccctgac
     9361 ctatcggcaa ggcgatattg tcccaggctt tttgttcaaa cgcttcatca acgaattttt
     9421 cacgcggcac ggcggcaagt gcattcagca cctgctcatc ctgaatacct tgcgcacgta
     9481 attgctccag aagtgcttgt acgcgtctgc ttaccattgc gtgccaactc ccacgctgtt
     9541 taaccagtct gaaaccacat cttgcgcgct atgcgcagtt aaatccacat gcagcggcgt
     9601 gatggagaca tagccctcat ctaccgcagc aaaatcggtc cccggaccgg catcacattt
     9661 accgcccggc gggccaatcc agtacagcgt attgccgcgc ggatcttgct gcgggatcac
     9721 ctgatctgcc ggatgtcgtg taccgcagcg cgtcacgcga atacctttga tttgatccaa
     9781 gggtaaatcc ggaacgttaa tattaagaat acgtccggtg cgcagcggct ctttacacag
     9841 tgcgcgcaaa attgaacagg ttaccgccgc ggcagtgtcg taatgtttat gcccgtcaag
     9901 cgagacggcc agcgccggaa aacctaaatg acggccttcc atcgcggcgg ctaccgtacc
     9961 ggaataaata acgtcatccc ccagattcgg cccggcgtta atgccggaca caacaatgtc
    10021 cgggcgcgga cgcatcagag cattcacgcc aagatagacg caatcggtcg gggttcccat
    10081 ttgcacagca atatcaccat tttcaaaggt aaacgtgcgc agggaggatt ccagtgtcag
    10141 agaatttgaa gcgccgctgc ggttacgatc gggggcgacc acctgaacgt cagcaaactc
    10201 acgcaaggct ttcgccagcg tttgtatacc gggtgcatgt accccgtcat cattactcag
    10261 caatatgcgc ataatcacct gttgtgttga taagttccct gacaacgctg gttgcaaaac
    10321 tacccgccgg aagccagaaa cggatctcta cggtgacgtc atcccaccaa ttccagctta
    10381 attgttgcgg atacagcaac atcgctctgc gcgcggcttc aactttttcg cgcaccagta
    10441 aagcttgtaa ttcagtttct gcggcgacag ctgccttttc gaatgccagc gcttcacgct
    10501 gagttcccca ttcgccacta cccggcaatg cggcggttat catcagctct ttatcgttga
    10561 cgcgacgctg taattccgcc agttcttcgg tggttgcgac aaaccagcta ccacgtccgg
    10621 ctaattgtag cgcatcgccg tcaacaactt gattaacgtc tgcttttttg aggcgctcag
    10681 caacaatctg attaaacaac gcactgcggg ctgccgacaa ccaaaaactc cgtttattgc
    10741 gatcgcgcac cggagtattg gtttgcgccc agcgctgcgc cccctgcaag ttgctaccgc
    10801 caatcccaaa acgttgggca ccgaagtagt tcggtacacc ttttacgcaa atatcgatca
    10861 gacgttgttc aacgtcatcg cgattgctca cttcgcgcag aaccagggta aaggcgttac
    10921 ctttcagcgc gcctaaacgc agcttgcgct tgtgccgcgc atactccagc acctggcagc
    10981 cttccagttg aaaggcgctc agatcgggca tttccttgcc tggcacgcga gcgcataacc
    11041 actgttccgt aacagcatgt ttgtcttttt gcccagcgaa gctgacttca cgggcatgaa
    11101 ttttcaggaa tttcgccagt gcatccgcca caaaacgggt attgcagccg tttttgagga
    11161 ttctaaccag aatatgctca ccttcaccat caggctcaaa gcccaaatct tccaccacca
    11221 caaagtcttc cggattggct ttcagcagcc cggtgccttg cggtttaccg tggaggtaag
    11281 tgagattatc aaactcaatc attttgttgc cttaatgagt agcgccaccg cttcacaggc
    11341 aatcccttcc ccacgtccgg taaatccaag tttttccgta gtagtggctt tcacgttaac
    11401 atcatccata tggcagccga gatcttcggc aataaacacg cgcatttgtg gaatgtgcgg
    11461 caacatcttc ggtgcctgag cgataatagt gacatcgacg ttgcccaggg tataaccctt
    11521 cgcctgaata cgacgccagg cttcgcgtag cagctcgcgg ctgtcggcac ctttaaatgc
    11581 cggatcggta tccgggaaca gcttgccgat atcccccagc gccgccgcgc caagcaatgc
    11641 atcggtcaac gcatggagcg ccacgtcgcc atcagaatgc gccagcaatc ctttttcgta
    11701 aggaatgcgt acgccaccaa tgataattgg gccttcaccg ccaaaggcat gtacgtcaaa
    11761 accgtgtcca attcgcatta tgtattctcc tgatggatgg ttcgggtgag gtaaaactcg
    11821 gccagtgcca aatcttccgg gcgcgtgact ttaatgttat ccgcacggcc ttcgaccaac
    11881 tgaggatgga atccgcaata ttccagcgcc gaggcttcgt cggtaatagt cgcgccttca
    11941 tttagagcgc gcgtcagaca gtcatgtaac agctcacgag ggaaaaattg cggcgtcagc
    12001 gcgtgccata agccgttgcg atcaacggta tgagcaatgg catttttgcc cggttcggca
    12061 cgtttcatcg tatcgcgcac tggtgcggct aggatccctc ccgtgcggct ggtttcgctc
    12121 aacgccaaca atcgcgcgag gtcatcctga tgcagacaag gacgagcggc gtcatgcacc
    12181 aatacccact gcgcgtcgcc agcggctttc aaacctgcca gcacggaatc ggcacgctca
    12241 tcaccgccat ctacaacggt gatttgcgga tgattcgcca gaggaagttg tgcaaaacgg
    12301 ctatcgccag gacttatggc aatgacgaca cgtttcaccc ggggatgcgc cagcagcgca
    12361 tgcaccgagt gttcaagaat ggtttgatta ccgattgaga gatattgctt aggacattcc
    12421 gtttgcattc gacggccaaa tccggccgcc ggaaccacgg cgcaaacatc caaatgagtg
    12481 gttgccatgt taattcccgg gctgatttat cgattgtttt gccccgcaga ctgtgcgcgc
    12541 ttcgacgcgt caggcaccag acgataaaaa gtttcgcccg gcctggtcat gctgagttca
    12601 ttacgcgcac gctcttcgag cgcctcctgg ccgccattga gatcgtcaat ttcggcaaaa
    12661 agttgatcgt ttcgcgcttt aagtttcgcg tttgtagctt gctgtgccgc cacatcatca
    12721 ttgacgcggg tatagtcatg tataccgttc ttaccgaacc acagcgaata ctgtagccag
    12781 accagaatag ccagcaacag cagcgttagt ttacccatcc tgccccctga aaaacggcat
    12841 catcatccca tgcatccgaa gacgactcta catcctctgt tggggatacc gcgacaacgc
    12901 gggcaaatgt accacatttg tccattgtta cgtataccca gggcgtgcag aacataatct
    12961 cattattagt tacggtttga attatgaaca gaggagacaa gaaagtacaa attagcccag
    13021 tagccacata aacagtgcgc caaacataat gcctactgtc accagggtga aaacaatact
    13081 gtagcgtagc tttccgtcca tcaatgaatg cagcgcaatc cccaccacta ccgcgacggg
    13141 catcagcgcc agaaagaaag gccaggtgta gataaagaag aacagcgtgt tagagccata
    13201 aatcaacatc ggcatcgcca gcgcaaataa ccaggagata aaaccgacca cggcaccagg
    13261 cagtgaccat gtggtttctt catcctcagt aaggctgtcg ttatttgtta gtgtaatgtt
    13321 atggctatta cgcatatttg atcctgttac tttgacgaac cgggcatgga aacccggtgg
    13381 tgtctcagga tctgataata tcgttctgtc tcaacagatc taataattgc tgtaccaaat
    13441 ttgttactaa ttgttcacca ttgagatgaa tttctgccga ttcaggcgct tcgtaaacgg
    13501 aatctattcc cgtaaagttg cgcagttcac cggcacgcgc tttcttatat aagccttttg
    13561 gatcgcgggc ttcgcaaatc gccagcggcg tatcgacaaa cacttcgata aagcgccctt
    13621 ctcctacgcg ttcacgaacc atctggcgtt cggcgcggtg tggcgagata aatgcggtca
    13681 gcaccaccag tccggcttca accatcaaat tcgccacttc accgacgcga cggatattct
    13741 ctttacgatc ggcatcgcta aaaccgagat cgctgcataa tccgtggcga acattatcgc
    13801 catccagcag atacgtactg acgccgagtt tatgtaacgc ctcctccagc gccccggcga
    13861 ccgttgattt accggacccg gagaggccgg taaaccacag cactacacca cgatgaccgt
    13921 ggtgtagctc gcgttgttgc acagtgaccg gatggctatg ccagacgacg ttttcgtcat
    13981 gcagcgccat tatttatccc ccagcaaatc gcgcgcgccc cagtgcggaa agtggcgacg
    14041 aaccagagca ttcaattcca gttcgaatgc actgaattca gatggcgcag cagttgcctg
    14101 gctaactggc tcgtgcacca taccggcacc cacggtcaca ttgctcaggc gatcgataaa
    14161 aatcagccca cccgtcaccg gattttgttg ataacgatct aacaccagcg gctcgtcaaa
    14221 agtgagatcc acgaggccga tcccattcag tggcaggttt tcaacttcac gctgggtaag
    14281 gttattaata tcaacctgat agcgaatgcc atcaacacgc gcgcgcgtct tcttaccggc
    14341 aattttgatg tcgtaactct gccctggaga aagcggctgt tccgccatcc ataccacatc
    14401 caccgacgcg ctctgcaccg ccggtaacgc ttcgtctgcc gccagcagca gatcgccacg
    14461 gctgatgtcg atctcatccg tcagcaccag ggtgatcgct tctccggcaa aggcttcttc
    14521 gcgatcacca tcaaaagtca cgatccgcgc gacgtttgat tccacaccag agggcagcac
    14581 ttttacacgt tgcccgactt ccacgcgacc ggatgccagc gttccggcgt agccacgaaa
    14641 atcgagattc gggcgattaa cgtactgcac cgggaagcgc attggctgag catccaccac
    14701 tcgctggatc tccacggttt ccagcacttc gagcagtgtc ggaccgctgt accacggcat
    14761 actttcactt tgcgatgcca cgttgtcgcc ttccagtgca gagagcggca caaagcggat
    14821 atccagatta cccggcagct gcccggcaaa ggtcagataa tcttcacgaa tacgggtgaa
    14881 cgtctcttca ctgtaatcca ccagatccat tttgttgatc gccacgacca gatgtttgat
    14941 ccccaacagt gtggagataa aactgtgacg acgggtttga tcgagcacgc ctttacgggc
    15001 atcgatcagt aagatcgcca gttcacatgt cgatgcgcca gtcgccatat tgcgagtgta
    15061 ctgctcgtgc cctggggtgt cggcgataat aaatttacgc ttctcggtag agaaatagcg
    15121 gtaggccaca tcaatagtga tgccctgttc gcgctcagct tgcaggccgt ccaccagcag
    15181 agccagatcc agcttttcgc cctgggtacc gtgacgctta ctgtcgttat gcagcgatga
    15241 gagctgatct tcatagattt ggcgagtatc gtgcagcagg cgaccaatca gagtgctttt
    15301 gccgtcatcg acgctgccac aggtcagaaa acgcagcagg cttttatgtt gttgcgcaat
    15361 catccaggct tcgacgccgc cttcattggc gatttgttgt gcaagtgcgg tgttcatctt
    15421 aaaaataccc ctgacgtttt ttcagctcca tagatcccgc ctggtcgcgg tcaatcacgc
    15481 ggccctgacg ttcactggtg gtggaaacca gcatctcttc gatgatttcc ggcagtgttt
    15541 gtgcatttga ctccaccgca ccggtcagcg gccagcagcc cagcgtacgg aaacgcacca
    15601 tccgtttttt aatcacttcg cccggttgca ggtcgatacg gttgtcatca atcatcatca
    15661 acataccgtc gcgttccaga accggacgtt ccgcagcgag atacagcggc acaatgtcga
    15721 tattttccag ccagatgtat tgccagatat cctgctcggt ccagttagag agtgggaaga
    15781 cgcggatgct ttcgcctttg ttaatttgcc cgttgtagtt gtgccacagc tccgggcgct
    15841 gatttttcgg atcccagcga tggaagcggt cacggaaaga gtagattcgc tctttagcgc
    15901 gggatttctc ttcgtcacgg cgcgcaccac cgaaggcggc atcaaaaccg tatttgttca
    15961 gcgcctgttt caggccttca gttttcataa tatcggtatg tttcgcgctg ccgtgcacga
    16021 atggattaat ccccatcgcc acgccttccg ggtttttatg caccagcagc tcgcagccgt
    16081 aggctttcgc cgtacgatcg cggaactcat acatctcgcg gaatttccag ccggtatcga
    16141 catgcagcaa cgggaaaggc agcgtacctg gataaaacgc cttgcgcgcc aaatgcagca
    16201 tgacgctgga atctttaccg atagagtaga gcatcaccgg atttgagaat tctgccgcca
    16261 cctcgcgaat aatgtggatg ctttccgcct ccagttgccg caggtgagta agtcgtattt
    16321 gatccataac cgttcctttg caataccgct attttcttgc catcagatgt ttcgactata
    16381 gggagcgtaa gagaacgaat gaaattacca attagaatga gtagttcctt aacggaataa
    16441 cgatttggca aagctaatat caaaaagtgc ttaaggcacc ggatttctgg cgtttaggaa
    16501 gatttgaaat tgttttagcg cagcggaagt ttcatactat ggcggtaaaa aaatttgcat
    16561 ggtatttaag gactcactat gttttccgca ttgcgccacc gtaccgctgc cctggcgctc
    16621 ggcgtatgct ttattctccc cgtacacgcc tcgtcaccta aacctggcga ttttgctaat
    16681 actcaggcac gacatattgc tactttcttt ccgggacgca tgaccggaac tcctgcagaa
    16741 atgttatctg ccgattatat tcgccaacag tttcagcaaa tgggttatcg cagtgatatt
    16801 cggacattta atagtcggta tatttatact gcccgcgata atcgtaagag ctggcataac
    16861 gtgacgggaa gtacggtgat tgccgctcat gaaggcaaag cgccgcagca gatcatcatt
    16921 atggcgcatc tggatactta cgccccgctg agcgatgctg acgccgatgc caatctcggc
    16981 gggctgacgt tacaaggaat ggatgataac gccgcaggtt taggtgtcat gctggaactg
    17041 gcagaacgcc tgaaaaatac gcctaccgag tatggtattc gatttgtggc gaccagcggc
    17101 gaagaggaag gaaaattagg cgctgagaat ttactcaagc ggatgagtga caccgaaaag
    17161 aaaaatacgc tgctggtgat taatctcgat aacttaattg ttggcgataa attgtatttc
    17221 aacagcggtg taaaaacccc tgaagcagta aggaaattaa cgcgcgacag ggcgctggca
    17281 attgcgcgca gtcacggaat agccgcaacg accaatccgg gtttgaataa aaattatccg
    17341 aaaggcactg ggtgttgtaa tgacgcagaa atattcgaca aagcgggcat tgctgtactt
    17401 tcggtggaag cgactaactg gaatcttggg aataaggatg gttatcagca acgcgcaaaa
    17461 acagccgcat tccctgcggg aaatagctgg catgacgtaa gactggataa tcaacagcat
    17521 attgataagg cacttcctgg aagaatagaa cgtcgctgcc gtgacgttat gcggataatg
    17581 ctacctctgg tgaaggagtt ggcgaaggcg tcttgatggg tttgaaaatg ggagctgggt
    17641 gttctaccgc aggggcgggg aattctaagt gatatccatc atcgcatcca gtgcgcccgg
    17701 tttatccccg ctggcgcggg gaactcttac tgcttggtat gcggaatcac accctgaacg
    17761 gtttatcccc gctggcgcgg ggaacacact aagcatacat aactgttttt aaacaaattt
    17821 attccgcatc aacaatctac caactaaatt aaaacatttc cttattttta aagaacggat
    17881 aacttattga ttatcaacag gaagaaaaga aaccaaacgt aacccatcca aatccaccgg
    17941 aatacgtctg ttttctcccc aggtctgaaa ttcaaaaccc gactcgatat tggtcgccca
    18001 ggccatcacc acatttccgc aaccagccag ttgggtaatt tgctgccaga tcatctcccg
    18061 aatacgtttt gatgtatcac caacatacac accggcacgc acttccagta gccagattgc
    18121 gagccgtcca cgtaagcgcg gagggacatt ttctgtaaca accacgacca tgctcatccg
    18181 ccgcgccccc ggtgaccact atcacccagc gtttcaggtt cagggatggc aggcggtaac
    18241 atatccggcg cgggttgtgg tggttcaatt tcacctgcag caaggacttc ctcaattaac
    18301 ggtattaatt tgcccgttaa cttagtgcta cggaaaatat cgcgacaggc taatctgact
    18361 tctttatcag gttctgcggg ttgcctcgct gctatttcaa atgcctttgg cacaaccgaa
    18421 tcaaatttaa tgatatcggc tatgtcataa acaaatgaaa gcggtttgcc actatggata
    18481 aacccaatag cgggcgcata tcccgcggct aatactgccg cttcagatat accgtacaga
    18541 catgatgtag cagcactgat gcagcgattc acaacatcgc ctttttccca gtctttagga
    18601 tcgtatttgc gaccattcca tttcacacca tattgtttcg ccagtaatgc atacgtctgg
    18661 cgaacgcgag atccctcaat tccacgtaac tgctccactg aacggcgagc tggcggtggt
    18721 tcacgaaaac gtaattcata cattttgcgc accaccttca accgtagatc ttccgttaaa
    18781 gccagctttg cctagtagag taatttatct gcccgcgccc ctccgggttg tccggaggag
    18841 taaacgcgaa cgcccgcttc accgacccag accagcagtg ttcccaccgt ggcggccaga
    18901 tggactgccg cgtgggaaac tcttgttccc ggttcgagca taatgcaggc gaccgatccc
    18961 actggaatat gcgtgcggat cccggttttg tcgatcagca cgaaagcgcc gtccagtacg
    19021 tcgatttgac cgtactggag gaagatcatg gaagtgcgat cttttaatgg gatcgggctc
    19081 agcggtacaa acgtcacgcc tctgctccgg gtttgatcag catcagacca caaccgaacg
    19141 cccggctttt gccatacccc tgactgagac gttgcagaaa taacccagga gcggtgaccg
    19201 taagcatccc cgtataatcc acgctactga actggatcag ttgccgggag ttttcccgcc
    19261 gcagttgctg ttgtcggtag gcatcaaccg aagtatccag cagtgtaaaa ccgcttctct
    19321 ccccctgcgc tgccagccag tccagcgcag cctgttgttg atgcaaccag acatcacttc
    19381 cttccacctg ccccctcacc tgccgtttcg cctccatcag caagtcgtgg cgtttgcccg
    19441 ctttacaaat tgttggattc gcccgcaggt tgaaacaaag ggattgtccg gtacgcaact
    19501 ctggcgcaaa tggtcggcat tcgatggtga aaatatcact ttccgccggg cgttcctgcg
    19561 acaggacaaa aaagcg
//
LOCUS       DAETQN010000080        18844 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.080, whole genome shotgun sequence.
ACCESSION   DAETQN010000080 DAETQN010000000
VERSION     DAETQN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.080"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(254..2125)
                     /gene="ppiD"
                     /locus_tag="K0W68_002809"
     CDS             complement(254..2125)
                     /gene="ppiD"
                     /locus_tag="K0W68_002809"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBI3074323.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2317..2589)
                     /gene="hupB"
                     /locus_tag="K0W68_002810"
     CDS             complement(2317..2589)
                     /gene="hupB"
                     /locus_tag="K0W68_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBI3074324.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2798..5152)
                     /gene="lon"
                     /locus_tag="K0W68_002811"
     CDS             complement(2798..5152)
                     /gene="lon"
                     /locus_tag="K0W68_002811"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBI3074325.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5340..6614)
                     /gene="clpX"
                     /locus_tag="K0W68_002812"
     CDS             complement(5340..6614)
                     /gene="clpX"
                     /locus_tag="K0W68_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBI3074326.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6740..7363)
                     /gene="clpP"
                     /locus_tag="K0W68_002813"
     CDS             complement(6740..7363)
                     /gene="clpP"
                     /locus_tag="K0W68_002813"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBI3074327.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7609..8907)
                     /gene="tig"
                     /locus_tag="K0W68_002814"
     CDS             complement(7609..8907)
                     /gene="tig"
                     /locus_tag="K0W68_002814"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBI3074328.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9251..9568)
                     /gene="bolA"
                     /locus_tag="K0W68_002815"
     CDS             complement(9251..9568)
                     /gene="bolA"
                     /locus_tag="K0W68_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBI3074329.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9873..10451
                     /locus_tag="K0W68_002816"
     CDS             9873..10451
                     /locus_tag="K0W68_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI3074330.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10495..11970
                     /gene="ampG"
                     /locus_tag="K0W68_002817"
     CDS             10495..11970
                     /gene="ampG"
                     /locus_tag="K0W68_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBI3074331.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12431..13378
                     /gene="cyoA"
                     /locus_tag="K0W68_002818"
     CDS             12431..13378
                     /gene="cyoA"
                     /locus_tag="K0W68_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HBI3074332.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13400..15391
                     /gene="cyoB"
                     /locus_tag="K0W68_002819"
     CDS             13400..15391
                     /gene="cyoB"
                     /locus_tag="K0W68_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HBI3074333.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15381..15995
                     /locus_tag="K0W68_002820"
     CDS             15381..15995
                     /locus_tag="K0W68_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBI3074334.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15995..16324
                     /locus_tag="K0W68_002821"
     CDS             15995..16324
                     /locus_tag="K0W68_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBI3074335.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16336..17226
                     /gene="cyoE"
                     /locus_tag="K0W68_002822"
     CDS             16336..17226
                     /gene="cyoE"
                     /locus_tag="K0W68_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBI3074336.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17411..17722
                     /locus_tag="K0W68_002823"
     CDS             17411..17722
                     /locus_tag="K0W68_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBI3074337.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17706..18248
                     /locus_tag="K0W68_002824"
     CDS             17706..18248
                     /locus_tag="K0W68_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3074338.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18304..>18844)
                     /locus_tag="K0W68_002825"
     CDS             complement(18304..>18844)
                     /locus_tag="K0W68_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBI3074339.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4216 a         4955 c         4580 g         5093 t
ORIGIN      
        1 ccgccgtcgg tttcgccaca gaagctgccg ccagcgcgct atgagacatt ccggcacagg
       61 ccagggacag ggtaatgagc agtgctttaa ttccgtgttt catactgttt tctccttgtt
      121 tgttaacagt ggaatcacct taacgggagt gaaaacagta aacaaatggc aatgttcaga
      181 aatggaaaag gccgcgaaag cgaccttttc ttgttacagc tgcaacgcat ttttttgcga
      241 ggcggcttca ggattattgc tgttccagcg catcgccaat tttgattttc gcctctttac
      301 gcaggttact catcagagct tcaaagacga tttgtgcgtt gttctgggtg ataccctgca
      361 ccatcgcttt tttctgatct tccggcattg aaccttgttt cacttcatcc agcgccagca
      421 gaaccacatt accttgcata tcggtcgcca taccgtagct cggtttgtct ttcgctggca
      481 gtggcagtgc aaacgccgcc tggctaatcg ggtcacgacc ggaacggctt aaggttttcg
      541 gctcgccaaa tttcagaccg gcagcctgca tagcttccgc ccctttgccg gctttcaaat
      601 caaccagcag tttctcagca tccactttcg cctgttgttc agctttgttg tgctgaacca
      661 gtgccttaac ttgttcctga acatctgcca acggtttcac cgcttccggt ttgtgctcgc
      721 tgatgcgcag cacgaatgcg cggtcgccgt ctacggtgat gatgtcagag ttgatgcccg
      781 gcgcgccgtt ttcacctacc agaccgccgt taaagatagc gtcggcaacc ggcttgaagt
      841 tcaactcttc cggcaggtta tctttgctga accaacccgt ctgagtggct ttaacgccgg
      901 cagcttgctc tgcaccggcc agagactcgg tgtcgttgct tgccgcatcg ctcactttct
      961 gctgcagcgc atagtacgca tcgagggctt tttcgtgttt cactttcgcc gcaatgtcgt
     1021 cacgtacttc gtctaacgat ttcactttcg ctggctgaat gtcgtccaga cgtacaatca
     1081 ggaaaccgac cgaagatttg atgacaccag acagttggcc tttttctttc agaccagcat
     1141 ttttcagttc gtcggggata gtggcatctt ctaaccaacc catatcgccg ccgttacggg
     1201 cagagataat atcagcagat ttttctttgg ctaatgcagc aaaatcaccg cctttattca
     1261 gctcatcaag taccgctttc gcttcatctt cagttttggt ctggatgatg ctgtagcggg
     1321 tacgctgcgg ctgggtgaat tgatcctgat gctgatcgta gtagctctgg atatccgcat
     1381 cgctaaccgg ttgctgcatc gttgcggcat ccagcttgat gtaactcacg cggaattgtt
     1441 ccggcgtcat gaaattgttt ttgttttgtt cgtagtagct ggcaatctcc tgttcggtca
     1501 caggctgctt cgccgccagc gcgttaacat cgatagtcgc ctcacgcacc acgcgttgtt
     1561 gcgcgaccag tgccgccagc tcgtcggttt cacctttcag cataaaatcg gtaccggcaa
     1621 caccgttaat cagctgttgg gtagtgagct ggttacgcag cgcctgggcg tactgatcgg
     1681 cggtcatccc catctggttg aggataccgt tatagcggct gttatcaaat ttgccgtcaa
     1741 cctggaaggc tggggtcgcg aaaatcgcct gtttaacctg ctcatcactg atacccagtt
     1801 tcagctcacg agcgtactga tccaacagcg cctcgtcgat cagacgattc agcacctgtt
     1861 gacgcagggt tttcatatag ccttcgttcg ctgccagctc ggagtattga tcgcccagct
     1921 gttgctgcat gcgattacgc tcgctgttaa aggcgttctc gaactgccca cggctgattt
     1981 cctggtcatt cacttttgcg gcgtagttat tgcctccgcc aatcaggtaa ccactcacgc
     2041 cggtcaatat gaacgacacg ataatgatac cgaaaataat cttgagcacg agactgtttg
     2101 cagccgtgcg taagctgtcc atcatggtgt aacaacactc cgctgtagat gactgtgtac
     2161 ctcacgcaac gtatcgatgc gcgcaaaggc ctattgtgac aagaaacggg ggcaattgtc
     2221 agcccacaag acacagaaag tcaccgaata caaataaaaa aggcacatca gtagatgcgc
     2281 ccttgaactt cgtcacatcc ccactgggga caacgcttag tttaccgcgt ctttcagtgc
     2341 tttacctgca cggaagctcg gtactttagc ggcagcgatg gtgatctctt taccggtctg
     2401 cgggttgcgg ccagtacggg cagcacgctc tttaacggca aaagtaccaa aacctaccag
     2461 tgctacatca tccccttctt tcagagattc agttacggaa gcaataatag catctaacgc
     2521 acggccagcc gcagctttag agatatcagc ccctgcagca atcttgtcga tcaattgaga
     2581 tttattcact cttctcttcc tctttataat ttatatcgca cttgaatcct tcaaggtacg
     2641 aacgcgcagc agttatatca ggccagccat ccccttacaa caccccttaa taagggcaag
     2701 cccgatccgc catctaactt agcgagacaa aaaaaggctg gcaagcccga attagcctgc
     2761 cagccctgtt tttattagtg cattttgcgc gaggtcacta ttttgcagtc acaacctgca
     2821 tgccagacgg ttcattttgc agcgccagag tcagaacttc ctcaatgcgc ttcacaggat
     2881 gaatgtccag atcggcaatt acgttgtcag gaatctcttc cagatcgcgt ttattttcga
     2941 acggaattag cactgtttta atcccgccgc gatgcgctgc caggagtttt tctttcaaac
     3001 caccgatcgg cagtacctga ccacgcagag tgatctcacc ggtcattgcc acatcggcac
     3061 gaaccgggtt accggtcagg caagaaacca gcgcggtgca catagcaata ccggcactcg
     3121 gaccatcttt cggcgtcgca ccttccggta cgtggacgtg gatgtcacgt ttttcgtaaa
     3181 aatcagggtt gatccccagt ttttccgcac gcgcacgaac caccgttaac gccgcctgaa
     3241 tagactcctg catcacttcg ccgagcgaac cggtataggt cagtttgcct ttacccggaa
     3301 cacatgcggt ttcaatggtc agcaagtcac cgcccacttc cgtccacgcc agaccggtta
     3361 cctgaccgac acggttttcg ttatcagcgc gaccatagtc gaaacgctga acaccgaggt
     3421 agtcatgcag gttatcgccg ttaatttcga tatgttttaa tgacttatcg agcagtaact
     3481 gcttaaccgc tttgcggcac agtttggaga tttcacgctc cagaccacgc acgcccgcct
     3541 cacgggtgta gtaacgaata atgccgataa tggcgctatc gtcgacggtc agctcacctt
     3601 ttttcagtgc attacgttca atctgcttcg gcagcaggtg acgtttggcg atgttcagtt
     3661 tttcatcttc ggtataaccg gagaggcgaa tcacttccat acgatccagc agcggtgccg
     3721 gaatgttcat ggagttcgac gtcgcgacaa acatcacgtc gctgagatcg taatccactt
     3781 ccaggtagtg gtcgctgaac gctacgttct gctctggatc cagcacttca agcagtgcag
     3841 aggccggatc gcctcgcatg tcagaagaca ttttgtcgat ctcatcgagc aggaacagcg
     3901 ggtttttcac gcccactttc gccattttct ggatcagttt acccggcata gaaccgatgt
     3961 aagtacggcg gtgaccacgg atttccgctt catcacgcac gccgcccagc gccatacgga
     4021 catatttacg cccggtggct ttggcaatgg actgaccaag agaggtttta cctacccccg
     4081 gcggccctac caggcagagg atcggtccct tgattttgtt gacacggctt tgaaccgcaa
     4141 ggtactcaag gatgcgatct ttcacgcgct ccagaccata atggtcggta tcaaggattt
     4201 cctgcgcctg acgcaggtct tttttgacct tgctgcgcgc attccacggt acctgtacca
     4261 tccagtcgat ataaccacgc actacggtcg cttctgccga catcggagac atcattttca
     4321 gcttctgcaa ctctgcttcc gctttctctt ttgcctcttt cggcatcttc gccgcgtcga
     4381 ttttgcgctt cagggcttcg ttttcgtccg gcgcgtcgtc catttcaccg agttctttct
     4441 gaatagcttt catttgctcg ttcagatagt actcacgctg ggatttctcc atctgctttt
     4501 taacgcggtt gcgaatgcgt ttctcaacct gcagcagatc gatttccgat tccatcattg
     4561 ccatcagata ttccagacgt tcgttaacgt cggacatctc cagaacggac tgtttgtcag
     4621 ccagtttcag cggcatatgt gcagcaatgg tatccgccag acgcgccgga tcgtcgatgc
     4681 tattcagcga cgtcagcact tctggtggga tttttttgtt cagcttgatg tagccttcga
     4741 actggctgat tgcagtacgc accagcactt cctgttcccg ctcatcaatg gtcggcgact
     4801 ccagatactc cgccttcgca gaaaagtgtt cgccattgtc agagagcgca gaaatacgcg
     4861 cgcgctgtaa cccctcgacc agcactttga cggtgccgtc aggcagtttc agcatctgca
     4921 atatagaggc cacggtcccg acggtgaaaa gatcgtttac acccggctca tccgttgaag
     4981 cttctttctg cgcgaccagc ataatttttt tatcatggtc catcgccgct tccagacaac
     5041 ggatagattt ttcccgcccg acaaataagg ggatgaccat gtgcggataa accaccacat
     5101 cgcgcagcgg caatacgggg atttcaatgc gttcagaacg ctcaggattc atagagctct
     5161 ctcttagttt aatttccgcc aggtaatcag atgacacgac tgtgcttcac gccatctatt
     5221 aacatgtacg tcagtatatg gggatgtttc ccccacattc aacgccgaga atagaggaaa
     5281 aattaaaggg gagataaaat cccccctttt tggttaacta attgtatggg aatggttaat
     5341 tattcaccag atgcctgttg cgcttccggc ttaccataaa tcagcaacgg tttgctttga
     5401 ccatcaatta ccgactcgtc gataaccact ttttcgacgt cttccatgga cggcagatcg
     5461 tacatggtat cgagcagtgc ggcttctacg atggaacgca ggccacgggc accggtttta
     5521 cgcgccatcg ctttcttagc gatagcatcc agcgcctcgt cacggaattc cagatccacg
     5581 ccttccagat taaacagcgc ctgatactgc ttggtcaggg cgtttttcgg ctctttgagg
     5641 atctgaatca gagcttcttc gctcagttca ttcaacgttg ctacaaccgg cagacgacca
     5701 atgaactcag ggataagacc aaacttgatc agatcttccg gttcaacctg cgccagcagc
     5761 tcgccttcgc ttgctttgtc ggacttcgct tttaccgtcg cgccaaaacc aatgccggag
     5821 ccggtttcta cacggtggga aatcacttta tccagaccgg caaacgcacc gccacaaata
     5881 aacaggatct tagaggtatc aacctgcaag aattcctgct gcggatgttt acgcccacct
     5941 tgcggtggaa cagcagctac cgtaccttcg atcagtttca acagtgcctg ctgtacgcct
     6001 tcaccggaaa cgtctcgggt aatggacggg ttgtctgact tacgagaaat cttgtcgatt
     6061 tcatcgatgt agacaatccc gcgctgcgct ttctgtacgt cgtaatcgca cttttgcaac
     6121 agcttctgaa tgatgttttc aacgtcttcg cccacataac cggcttcggt cagcgtggtt
     6181 gcgtcggcca tggtgaacgg gacgtccagc aggcgcgcca gcgtttcagc cagcagcgtt
     6241 ttaccggaac cggtcggacc gatcagcaga atgttacttt tgcccaactc gacaccattg
     6301 ctggtatcgc cgttgcgcag acgtttgtaa tggttgtata ccgcgaccgc cagcactttt
     6361 ttcgcctgtt cctggccgat aacgtaatcg tccaggtggt tgcgaatttc atgcggcgtc
     6421 ggtagcgcac tgcgttcacg atgcggtgca acttctttaa tctcttcgcg aatgatgtcg
     6481 ttacataaat caacacattc gtcgcagata tacacggatg gaccggcaat cagcttgcgc
     6541 acttcatgct ggcttttgcc gcaaaaagag caatacagca atttgcctga gccatctttg
     6601 cgtttatctg tcatgggtca aaacctcttc tttgttcttt gtgccgcaca cgacgacgca
     6661 aatgccattc tcaggcgcaa gccgcttaca gcgttgtgcc gccctggata agtatagcgg
     6721 cacagttgcg cctctggcat caattacgat gggtcagaat cgaatcgacc agaccgtatt
     6781 ccaccgcttc aggggcggaa aggaagcgat cgcgctcggt atcacgttca atctgttcta
     6841 atgattgacc cgtatgaagc gccataagtt cattcatgcg ccctttaact ttcagaattt
     6901 cacgggcatg aatttcgata tcggtcgcct ggccctggta gccgcccaac ggttggtgaa
     6961 tcatcacgcg cgaattcggc aggcaaaaac gtttaccttt tgcccctgcg gtcagcaaga
     7021 aagcgcccat cgaggccgcc tggcccatac agatggtgct gacatcaggc ttgataaact
     7081 gcatggtgtc atagatagac atcccggcag tgatcacccc gcctggggag ttaatgtaca
     7141 gatagatatc tttttctggg ttttccgctt ctaggaacag catctgcgcc acaatcaggt
     7201 tcgccatgtg gtcttcaacc tggccagtca gaaaaatgac gcgttcctta agtagacgag
     7261 aatagatatc aaaagagcgc tcaccgcgtg aggtctgttc aatgaccatc ggcaccagcg
     7321 ccatatgggg tgcaaagtta tctcgttcgc cgctgtatga catttccgtc tcctggataa
     7381 aattgaaaaa acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg
     7441 tgttatgggt acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca
     7501 ccgaaagagg cttttcgcac ggttccatgc aaaattcatg acaaaaaaaa gcccaccacc
     7561 tggcggtgac gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg
     7621 gttcatcagc tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc
     7681 ttcaacagcc tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt
     7741 gctgtagaac tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag
     7801 gcctttcacg cgctcttcgt cagctttcag ttcgttggtg cggataactt cgcccagcag
     7861 caggccaaca actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag
     7921 agcttgtttt tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc
     7981 gctgtcgatc agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc
     8041 ctgagactta acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac
     8101 ttcagcgcgc agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc
     8161 agtcagttcc ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc
     8221 tttacctttc agattttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc
     8281 ttcgccagct ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc
     8341 catcgccagt acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc
     8401 ggtgaagtcg atggttacgc ggtcttctgc ttcaacggcg ccgtcttttt ctttccaggt
     8461 cgcctgctgt ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac
     8521 gatcggtttt tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc
     8581 aaactctaca gagtaggtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg
     8641 tgcgccagcc ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag
     8701 gtcacccaga acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg
     8761 cactttgcct ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct
     8821 tttaacagcg gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc
     8881 ttgagtggtt tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca
     8941 actcttaccc cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa
     9001 tcacattccc tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc
     9061 ggcatcactt ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt
     9121 cccgggcaat tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg
     9181 gcccgcaggc cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc
     9241 aaatgcgttt ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt
     9301 cctgcaaccc ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag
     9361 tagagagttc ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac
     9421 ccgtaaaacg atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat
     9481 tgtgacgata gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt
     9541 tttcttctat ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca
     9601 tccatccctt taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt
     9661 taggtttcgt cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac
     9721 acaggcctcg catcagcgtt tcttttacca ggggtgatta tttcgccaac atgatgaatt
     9781 tacatgcctt acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc
     9841 aaacacgctt aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag
     9901 ttgctctgtt tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga
     9961 cgattacact gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg
    10021 ccgatcagcg taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc
    10081 tgaccgcctc ccgagatctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg
    10141 cgcgtggtta catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac
    10201 tgtatgctga cgtgtcccag ggcaacgtgc gctacaacat cgcgaccaaa gcggatatcg
    10261 ccatcatcgc taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca
    10321 acgttgaagg tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc
    10381 tgaccgatac catcgctgat atgtctcagg acaccagcat ccacgaattc atcaagcaga
    10441 acgcgcgtta atttctgccc tctggcccgg tgcaaaccgg gcctgtagac gcccatgtcc
    10501 agtcaatatt tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt
    10561 gcttccgggc taccgctcgc cctgacatcc ggtaccttac aggcctggat gacggtcgag
    10621 aatattgatc tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa
    10681 ttcctctggt caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt
    10741 tggctgctcg ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa
    10801 cctggcaccc aactccgctg gatggcggca ctggcggtgg tgatcgcttt ttgctcggcc
    10861 tcccaggata ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt
    10921 gcgggcgcgg caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggctta
    10981 gccctgtggc tggcagataa atggctgggc tggcagggca tgtactggtt gatggcggcg
    11041 ctgttgatcc cttgtattat cgcgacgttg cttgcaccag aaccgaccga caccattcct
    11101 gtgccaaaaa cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat
    11161 aatgcctggc ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg
    11221 agcctgacaa ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg
    11281 gttaacaaaa cgcttggctt actggcgacc attgtgggcg cattgtacgg tgggattttg
    11341 atgcagcgcc tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct
    11401 aacgctggtt actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc
    11461 gtctttttcg aaaacctctg tggcgggatg ggcacatcag cctttgtcgc actgttaatg
    11521 acgctgtgta ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct
    11581 gtaggacgag tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg
    11641 acattctatc tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc
    11701 cgccagacgc ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg
    11761 acaggttatg cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg
    11821 ttactgctgt tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg
    11881 ctggaagtcg gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctggattat
    11941 ctggctctac gaaaaacgca tctgacgtaa tctgtaaata ttatttagct gtagtaatca
    12001 ctcgccgtaa ttattacggc taaataaaca tcagtatcgc ttaataatat ttgttctttt
    12061 gtgcggctta gcgtttggaa attattgacg caatttatga attctatttt ttcttacacg
    12121 attcagctaa tgagtcttta ttttcccccc acccaattgt caccataatg ataattattt
    12181 gttaaacaat tgttttattc aagattggtt ataccaattg cccgtccaaa ctccttctgc
    12241 actccccttt cgttataacg cccttttgca acagcttctt aaaatcaact tgatatgttt
    12301 tgcaacatat gtaacctgac agccaaatcc aagtaacagg aatttaatca tgtttacagt
    12361 aatgtaacct ccccgtaaaa tgcccacaca ctttaaacgc caccagatcc cgtggaattg
    12421 aggtcgttaa atgagactca ggaaatacaa taaaagtttg ggatggttgt cattatttgc
    12481 aggcactgta ttgctcagtg gctgtaattc tgcgctgtta gatcccaaag gacagattgg
    12541 tctggagcaa cgttcactga tactgacggc atttggcctg atgttgattg tcgttattcc
    12601 cgcaatcttg atggctgttg gtttcgcctg gaagtatcgt gcgagcaata aagatgctaa
    12661 gtacagcccg aactggtcac actccaataa agtggaagct gtggtctgga cggtacctat
    12721 cttaatcatc atcttccttg cggtactgac ctggaaaacc actcacgctc ttgagcctag
    12781 caagccgctg gcacacgacg agaagcccat taccatcgaa gtggtttcca tggactggaa
    12841 atggttcttc atctacccgg aacagggcat tgcaaccgtg aatgaaatcg ctttcccggc
    12901 gaacactccg gtgtacttca aagtgacctc caactccgtg atgaactcct tcttcattcc
    12961 gcgtctgggt agccagattt atgccatggc cggtatgcag actcgcctgc atctgatcgc
    13021 caacgaaccc ggtacttatg acggtatctc cgccagctac agcgggccgg gcttctcagg
    13081 catgaagttc aaagctattg caacaccgga tcgcgccgaa ttcgaccaat gggtcgcaaa
    13141 agcgaaacag tcgccgaaca ccatgtctga catggctgcg ttcgaaaaac tggccgcgcc
    13201 tagcgaatac aaccaggtgg aatatttctc caacgtgaaa ccagacttgt ttgccgatgt
    13261 gattaacaag tttatggctc acggtaagag catggacatg acccagccag aaggtgagca
    13321 cagtgcacac gaaggtatgg aaggcatgga catgagccac gcggaatccg cccattaaag
    13381 gggttgagga agaataaaga tgttcggaaa attatcactt gatgcagtcc cgttccatga
    13441 acctatcgtc atggttacga tcgctggcat tattttggga ggtctggcgc tcgttggcct
    13501 gatcacttac ttcggtaagt ggacctacct gtggaaagag tggctgacct ccgtcgacca
    13561 taaacgcctc ggtatcatgt atatcatcgt ggcgattgtg atgttgctgc gtggttttgc
    13621 tgacgccatt atgatgcgta gccagcaggc tcttgcctcg gcgggcgaag cgggcttcct
    13681 gccacctcac cactacgatc agatcttcac cgcgcacggc gtgattatga tcttcttcgt
    13741 ggcgatgcct ttcgttatcg gtctgatgaa cctggtggtt ccgctgcaga tcggcgcgcg
    13801 tgacgttgcg ttcccgttcc tcaacaactt aagcttctgg tttactgttg ttggtgtgat
    13861 tctggttaac gtttctctcg gcgtgggcga atttgcgcag accggctggc tggcctatcc
    13921 accgctatcg ggaatagagt acagtccggg agtcggtgtc gattactgga tatggagtct
    13981 ccagctatcc ggtataggta cgacgcttac cggtatcaac ttcttcgtta ccattctgaa
    14041 gatgcgcgca ccgggcatga ccatgttcaa gatgccagta tttacctggg catcactgtg
    14101 cgcgaacgta ctgattattg cttccttccc aattctgacg gttaccgtcg cgttgttgac
    14161 cctggatcgc tatctgggca cccatttctt taccaacgat atgggtggca acatgatgat
    14221 gtacatcaac ctgatttggg cctggggcca cccggaagtt tacatcctga tcctgcctgt
    14281 tttcggtgtg ttctccgaaa ttgcggcaac cttctcgcgt aaacgtctgt ttggttatac
    14341 ctcgctggta tgggcaaccg tctgtatcac cgtgctgtcg ttcatcgttt ggctgcacca
    14401 cttctttacg atgggtgcgg gcgcgaacgt aaacgccttc tttggtatca ccacaatgat
    14461 tatcgccatc ccgaccgggg tgaagatctt caactggctg ttcaccatgt atcagggccg
    14521 catcgtgttc cattctgcga tgctgtggac catcggtttt atcgtcactt tctcggtggg
    14581 cgggatgact ggcgtgctgc tggcagtacc gggcgcggac ttcgttctgc ataacagcct
    14641 gttcctgatt gcgcacttcc ataacgttat catcggcggc gtggtcttcg gctgcttcgc
    14701 agggatgacc tactggtggc ctaaagcgtt cggtttcaaa ctgaatgaaa cctggggtaa
    14761 acgcgcgttc tggttctgga tcatcggctt cttcgttgcc tttatgccgc tgtatgcgct
    14821 gggcttcatg ggcatgaccc gtcgtttgag ccagcagatt gacccgcagt tccacaccat
    14881 gctgatgatt gcagctagcg gtgcagtact gattgcgctg ggtattctct gcctcgttat
    14941 tcagatgtac gtttctattc gcgaccgcga ccagaaccgt gacctgactg gcgacccgtg
    15001 gggtggccgt acgctggagt gggcaacctc ttccccgcct ccgttctata actttgccgt
    15061 tgtgccgcac gttcacgagc gtgatgcatt ctgggaaatg aaagagaaag gcgaagcgta
    15121 taaaaagcct gaccactatg aagaaattca tatgccgaaa aacagcggtg caggtatcgt
    15181 cattgcagct ttctccacca tcttcggttt cgccatgatc tggcatatct ggtggctggc
    15241 gattgttggc ttcgcaggca tgatcatcac ctggatcgtg aaaagcttcg acgaggacgt
    15301 ggattactac gtgccggtgg cagaaatcga aaaactggaa aaccagcatt tcgatgagat
    15361 tactaaggca gggctgaaaa atggcaactg atactttgac gcacgcgact gcccacgcgc
    15421 acgaacacgg gcaccacgat gcaggcggaa ccaaaatctt cggattttgg atctacctga
    15481 tgagcgactg cattctgttc tcaatcttgt ttgctaccta tgccgttctg gtgaacggca
    15541 ccgcaggcgg cccgacaggt aaggacattt tcgaactgcc gttcgttctg gttgaaactt
    15601 tcttgctgtt gttcagctcc atcacctacg gcatggcggc tatcgccatg tacaaaaaca
    15661 acaaaagcca ggttatctcc tggctggcgt tgacctggtt gtttggtgcc ggatttatcg
    15721 ggatggaaat ctacgaattc catcacctga ttgttaacgg catgggtccg gatcgcagcg
    15781 gcttcctgtc agcgttcttt gcgctggtcg gcacgcacgg tctgcacgtc acttccggtc
    15841 ttatctggat ggcggtgctg atggtacaaa tcgctcgtcg cggcctgacc agcactaacc
    15901 gtacccgcat catgtgcctg agcctgttct ggcacttcct ggatgtggtt tggatctgtg
    15961 tgttcactgt tgtttatctg atgggggcga tgtaatgagt cattctaccg atcacagcgg
    16021 cgcgtcccat ggcagcgtaa aaacctacat gacaggcttt atcctgtcga tcattctgac
    16081 ggtgattccg ttctggatgg tgatgacagg agctgcctct ccggccgtaa ttctgggaac
    16141 aatcctggca atggcagtgg tacaggttct ggtgcatctg gtgtgcttcc tgcacatgaa
    16201 taccaaatca gatgaaggct ggaacatgac ggcgtttgtc ttcaccgtgc taatcatcgc
    16261 tatcctggtt gtaggctcca tctggattat gtggaacctc aactacaaca tgatgatgca
    16321 ctaagagcgg cggttatgat gtttaagcaa tacctgcaag taacgaaacc aggcatcatc
    16381 tttggcaacc tgatctcggt gattggggga ttcctgctgg cctcaaaggg cagcattgat
    16441 tatcccctgt ttatctacac gctggttggg gtgtcactgg ttgtggcgtc gggttgtgtg
    16501 tttaacaact acatcgacag ggatatcgac agaaagatgg aaaggacgaa gaatcgggtg
    16561 ctggttaaag gcctgatctc tcctgctgtc tcgctggtgt acgccacctt gctaggcttt
    16621 gctggcttta tgctgctgtg gtttggcgcg aatccgctgg cctgctggct gggggtgatg
    16681 ggctttgtgg tttatgtcgg cgtttatagc ctgtacatga aacgccactc ggtctacggc
    16741 acattgattg gttcgctctc cggcgctgcg ccgccggtga ttggctactg tgcagtaacc
    16801 ggcgagttcg atagcggcgc agcgatcctg ctggctatct tcagcctgtg gcagatgcct
    16861 cactcctatg ccatcgccat tttccgcttt aaggattatc aggcggcaaa cattccggta
    16921 ttgccagtgg taaaaggcat ttcggtggcg aagaatcaca tcacgctgta tatcatcgcc
    16981 tttgccgttg ccacgctgat gctctctctt ggcggttacg ctgggtataa atatctggtg
    17041 gtcgccgcgg cggtgagcgt ctggtggtta ggtatggccc tgcgcggtta taaagttgct
    17101 gatgacagga tctgggcgcg caagctgttc ggcttctcta tcatcgccat cacagccctc
    17161 tcggtgatga tgtccgttga ttttatggtg ccggactcgc atacgctgct ggctgctgtt
    17221 tggtaacaca acctctctat taaaaaggtg ctacggcacc ttttttattg tctgagatca
    17281 gagatacggc taataaccgt atgtcttgac agagtgagca gatgaacata aattaatcat
    17341 acggttgata gccgtattat gtatgagatc gactccctac gatttcattc agttaatcaa
    17401 agaggtcata atgcctggaa aaacagccac tctacccgat gtcgataaaa cgctgaaaaa
    17461 tgcgcgggtc gaactcaaaa ctagtcccga cgccaaaaac aaacttcgtg aagctgcgca
    17521 agccgttggg gtagatttaa gcgcttttat tttgagcgct gctatggaac gcgccgaaag
    17581 tgtgcttgat aaccaacgtc gccgtgagct ttcgaatcaa agctgggaac tgatgaacca
    17641 actcatcgct gaacctgctc aaccgacgct cgccctcaag gcgttaatga aaaggaaaaa
    17701 cagcgatggt cgacaagcat gaagagatta ctctgcccat agtcctctcc tgtaattatc
    17761 agtctgatat tacttatcct gggcaaaaac agtttgattg cggtaaccct gttatcgata
    17821 aatttgtacg cgcatcgcta aagaaaagtg tgcgtaatag cgactgtgcg gctaaagcac
    17881 ttattgacag acaaagtggt gaactgatcg gcatctgtac ttttacggca tattcgctgg
    17941 aaaaacaacg cgtttctggc gtccttcagg gttcacaacc ttcagaaatt ggtgttgtca
    18001 gattagtcat gttgggggta gcacggaagt atcaaaagcg gggctttggt caggacctac
    18061 tatgtgattt ttttgaacat gtaaaaataa ttcaccaggc attaccaatt aaaggggttt
    18121 atcttgatgc tgaccctgcc gccattaatt tttatgctcg tctcggcttt gttcagcttt
    18181 cagcgacacc aaatgctttt ggtgctgtac ctatgttttt ggcgattcag catattctcg
    18241 cggcttagat tattatcgcc acccgccagg gcaagttttg ccttagccgg gggctgagca
    18301 gcgttaccac ttttgttcaa ttgcccccgc tttcagcaac tccacatcct tgccgttaat
    18361 ggttgccgtt gcggcaacga ttgtggtcct tacagttgca caagattttc gctcaaattc
    18421 cggtttataa agaataagag acgaattact ggtaaatgaa ccaaataggt aatcagtaaa
    18481 gctaatattc agccggttta aaccgaaagg cgcgaagcca accgtttggt taatctcctt
    18541 accggatggg cgacgaatgc tgaatgtcac gcgaagattg gccacttcgc ctaatttatt
    18601 ggtggtggcg aagaggctat ccatgcagga aaaaccttta tctaaaaaat tatctcgcat
    18661 cccagaacgg gtaaccgtaa tcacgggtaa cgtatcgtca accgtttttc tgtcaatcat
    18721 gtcttttcga caagtagcca gaaagtcttc cgggccacca taaatgtgga cacctttatc
    18781 aagatattcc ttcgctttct tacaatctac ctcaacatac tgaccattgg cgtacataat
    18841 gccc
//
LOCUS       DAETQN010000081        18523 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.081, whole genome shotgun sequence.
ACCESSION   DAETQN010000081 DAETQN010000000
VERSION     DAETQN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.081"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1710
                     /locus_tag="K0W68_002826"
     CDS             <1..1710
                     /locus_tag="K0W68_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBI3074340.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="K0W68_002827"
     CDS             1711..3360
                     /locus_tag="K0W68_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBI3074341.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="K0W68_002828"
     CDS             3365..4141
                     /locus_tag="K0W68_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBI3074342.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="K0W68_002829"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="K0W68_002829"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HBI3074343.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="K0W68_002830"
     CDS             complement(5430..6752)
                     /locus_tag="K0W68_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HBI3074344.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="K0W68_002831"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="K0W68_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HBI3074345.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="K0W68_002832"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="K0W68_002832"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HBI3074346.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="K0W68_002833"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="K0W68_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HBI3074347.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="K0W68_002834"
     CDS             complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="K0W68_002834"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HBI3074348.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11632..14481
                     /gene="rcsC"
                     /locus_tag="K0W68_002835"
     CDS             11632..14481
                     /gene="rcsC"
                     /locus_tag="K0W68_002835"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBI3074349.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="K0W68_002836"
     CDS             complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="K0W68_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBI3074350.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="K0W68_002837"
     CDS             complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="K0W68_002837"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBI3074351.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4526 a         4535 c         4867 g         4595 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga
     9721 atatcaccct gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct
     9781 gaggctttgg tggtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca
     9841 cagccgttgt cctctatcga aatggcctgt tgtgagtcgc tgtattgcca ggtttgaatg
     9901 cgaattttcc ctcgtgcgct gatagcctgg acggcattga tcaggatatt cagtagtacc
     9961 tgtttgagca gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg
    10021 aagtcgaccc gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat
    10081 gcattgaggc tgacttgttg ccattgactg tggcgtggac gtgaaaattc gagcaattgc
    10141 tgaataactt tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt
    10201 attgggtcac tggtttgttg gcgcaagatc tgtacataac cacgaatagc cgttaacgga
    10261 ttacgtactt catgcgcgac gccagccatc agctcaccca gtgtggcgag gcgttctgct
    10321 tgcgccatgc ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca
    10381 cctatcattt caccgtgcgt gttatgaata cgactggtag tgacactgag ttcaatggtg
    10441 cggtcacgac ctggaaaact gatctccagc gccacatgtt cggtgccatg ttccagcgta
    10501 tccagtactg gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc
    10561 agttcatggc gttgatagcc agtgataact tctgctgctg ggttcatggt ggttacatca
    10621 ccctggcggt caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt
    10681 gtccgcgttt cacgcagtgc ctgggcgagg ttattaacac tctgactgat ttgccccatt
    10741 tcaccgggca attgtggtaa tcgagtggga atattttgtg ccagagtcga gaggccatcg
    10801 gtgatgatat caatattggc gctcaggcga cgggagaaaa ggacaatcaa tagcaggctt
    10861 atcagcaaat cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg
    10921 cgaatatctt cggttaattc attggcccag atatagccga ggatttcacc attacgctca
    10981 atggggagca ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata aaccaaaggg
    11041 gtatttgtac gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gccgacatta
    11101 ttctgatata gcgctgaagg cgcgtaggtg attatcgcat ccagcatttt gttgtaataa
    11161 ccagcaccta tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca
    11221 tttaatgcgc ggatacgctc ctcacgtggt aagtcgatgt agagatcata gcgatcgcct
    11281 agtgcctgat taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact
    11341 gctgaacgtc cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc
    11401 aggattgcca tcaggatcat ttgattgcgt aagcggcgtg gataaatcca cttcatataa
    11461 tgcatgctga aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat
    11521 gatttctcgg cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta
    11581 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
    11641 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
    11701 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
    11761 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
    11821 caacgcacct ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca
    11881 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
    11941 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag
    12001 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctaaac
    12061 cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg
    12121 ccagtggaac gcgataccgc gttgaaagct ttgcatgaac gcatcaataa atatcgaaat
    12181 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
    12241 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
    12301 ggtgtcgagc agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg
    12361 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
    12421 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
    12481 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
    12541 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
    12601 gtgcttgccg gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt
    12661 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
    12721 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
    12781 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
    12841 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
    12901 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
    12961 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
    13021 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
    13081 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
    13141 gtcgatcggc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
    13201 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
    13261 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
    13321 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
    13381 atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc
    13441 ggctgtatag ttttgcatgt ccgcgctgat ggcgattatc tctctatccg tgttcgcgat
    13501 accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
    13561 ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
    13621 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
    13681 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
    13741 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
    13801 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
    13861 gaagatgtgt taattactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg
    13921 accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac
    13981 agtgtggctg ccccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
    14041 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac
    14101 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
    14161 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gtgtcgatgc gcttaatgta
    14221 cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
    14281 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
    14341 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg
    14401 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
    14461 aggaaatcgc gggaatcgta ggacggataa ggtgtttatg ccgcatctgg cattcagtgc
    14521 aaatgccaga tgcgacgctg acgcgtctta tctggcctac tttgatgtaa tctgttgaaa
    14581 taatgggaat cgtaggacgc ataaggcgtt tacgccgcat tcggcaatcg gtgcaaatgc
    14641 cagataagac actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac
    14701 ttaacgtcac tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca
    14761 tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga
    14821 tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga
    14881 gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg
    14941 gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac
    15001 cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt
    15061 tgttgttcat agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga
    15121 ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat
    15181 gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa
    15241 cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt
    15301 catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc
    15361 ttgacataac tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga
    15421 atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat
    15481 acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca
    15541 gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg
    15601 gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc
    15661 agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg
    15721 acgcacaatt gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag
    15781 ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt
    15841 aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata
    15901 ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct
    15961 tcttcctctt caacttccgg gccagcggcg agcattttga tatgcacaga gtagcgtgta
    16021 ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg
    16081 ctcagccaga atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata
    16141 aacgggaagt gcaaattatc catttcatga atacttacgc cttctccggt gtccagaatg
    16201 cggaatgtca ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc
    16261 aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg
    16321 cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct
    16381 ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag
    16441 aacagcaccg tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca
    16501 tcgaccagcc gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg
    16561 agtttagccg cgctctccgc cagggactgt gcaggttctt tcagcgcatc gccaatgttt
    16621 ttcataaagg tcatccggcc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt
    16681 ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc
    16741 gcgacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata
    16801 atcccctgat gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa
    16861 tgatcggcaa ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg
    16921 ccgagcggca gcagtgagac tatcttttca ttgattgccc gtaaaatgcg caattcgtta
    16981 ttgaccgccg tgttgggtag actttctgta ctgcgactgg agaaatggcg gaatgtggta
    17041 tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt
    17101 tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta
    17161 gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc
    17221 gttccttctt tctcattatc attgtctccc gttgccgtcg tgtctggctc aaggcggaaa
    17281 ctgtccagcg gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc
    17341 gttgccagat gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc
    17401 caggccaggc ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc
    17461 aacatttctg cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag
    17521 gtgagatctt tgaggggcag ggttgagatc agtaccagac tgttatcctg accattcagg
    17581 taatacatcg accacggtac attttctgca ccccacaatg tatccagata agtggacatc
    17641 cgctgagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg
    17701 cggcgtggtt tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg
    17761 ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc
    17821 caggtatcga cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct
    17881 ttcagccagg cgttaacggc gctttgtacc attaccccca tcgtcaccag taacacaatg
    17941 atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc
    18001 gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg
    18061 cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct
    18121 cccgtgatat ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta
    18181 caaataacct acaaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat
    18241 tatgctgatg ttaattattt gtgaaatagt tgacaagagt tatagttttt ctgtggtagc
    18301 acagaataat gaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg
    18361 aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat
    18421 agcgggagat attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata
    18481 cttaagaata agttattgat tttaaccttg aattattatt gct
//
LOCUS       DAETQN010000082        18514 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.082, whole genome shotgun sequence.
ACCESSION   DAETQN010000082 DAETQN010000000
VERSION     DAETQN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.082"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            76..288
                     /gene="cspH"
                     /locus_tag="K0W68_002838"
     CDS             76..288
                     /gene="cspH"
                     /locus_tag="K0W68_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HBI3074352.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            735..1040
                     /locus_tag="K0W68_002839"
     CDS             735..1040
                     /locus_tag="K0W68_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074353.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1147..1791
                     /gene="gfcB"
                     /locus_tag="K0W68_002840"
     CDS             1147..1791
                     /gene="gfcB"
                     /locus_tag="K0W68_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HBI3074354.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1788..2534
                     /locus_tag="K0W68_002841"
     CDS             1788..2534
                     /locus_tag="K0W68_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBI3074355.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2534..4630
                     /locus_tag="K0W68_002842"
     CDS             2534..4630
                     /locus_tag="K0W68_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBI3074356.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4676..5815
                     /locus_tag="K0W68_002843"
     CDS             4676..5815
                     /locus_tag="K0W68_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBI3074357.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5803..6249
                     /gene="etp"
                     /locus_tag="K0W68_002844"
                     /gene_synonym="yccY"
     CDS             5803..6249
                     /gene="etp"
                     /locus_tag="K0W68_002844"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBI3074358.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6269..8449
                     /gene="etk"
                     /locus_tag="K0W68_002845"
     CDS             6269..8449
                     /gene="etk"
                     /locus_tag="K0W68_002845"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HBI3074359.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8564..9862)
                     /gene="appA"
                     /locus_tag="K0W68_002846"
     CDS             complement(8564..9862)
                     /gene="appA"
                     /locus_tag="K0W68_002846"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBI3074360.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="K0W68_002847"
     CDS             complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="K0W68_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBI3074361.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10047..11183)
                     /gene="appB"
                     /locus_tag="K0W68_002848"
     CDS             complement(10047..11183)
                     /gene="appB"
                     /locus_tag="K0W68_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBI3074362.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11195..12739)
                     /gene="appC"
                     /locus_tag="K0W68_002849"
     CDS             complement(11195..12739)
                     /gene="appC"
                     /locus_tag="K0W68_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBI3074363.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12873..13730)
                     /locus_tag="K0W68_002850"
     CDS             complement(12873..13730)
                     /locus_tag="K0W68_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBI3074364.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="K0W68_002851"
     CDS             complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="K0W68_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBI3074365.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14122..14709)
                     /locus_tag="K0W68_002852"
     CDS             complement(14122..14709)
                     /locus_tag="K0W68_002852"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBI3074366.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="K0W68_002853"
                     /pseudo
     CDS             complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="K0W68_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="K0W68_002854"
     CDS             complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="K0W68_002854"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBI3074367.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="K0W68_002855"
     CDS             complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="K0W68_002855"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBI3074368.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4544 a         4913 c         4782 g         4275 t
ORIGIN      
        1 gcggtcttaa atatcgctgg ctttatttta ttaaccccgt atagtgcagc gcagtatttt
       61 cagtaaggaa tttgtttgtc ccgtaaaatg acaggaattg tcaaaacctt tgatcgcaaa
      121 agcggcaaag gattcattat cccctccgac ggtcgtaaag aagttcaggt ccatatttcc
      181 gcattcactc cccgcgacgc agaagtgctt ataccaggat tacgtgtgga attttgccgc
      241 gtaaatggcc tgcgaggacc aacagcggca aatgtttatc tctcttaata tgacgctgct
      301 tgtttaaagc tgactggttt tattggaata gtgcaatagt caacatcata cattataata
      361 tcagttactt ttccacaaag aaactaatcc atttaagcga atatctctgc acctaatagt
      421 tataaatgcc atttaacagc cagttatagt accacttgaa actatattac caacaataat
      481 taatagcttt cgtaacaatt tccttatctt taatttgtat tttgctaaga tttatcttat
      541 gctcacaagt cgtatttcca gaggggatcg ttaataatct tatatttccc tctgcgcacc
      601 tttcctaccc gcagtatcag tatatagtgc aagcggtatg aggaaaggga gatttcacaa
      661 ggatgttata ctcaaagtag acaatcctga tctcaagcgt acgtattgtc catgcgatga
      721 ataaaggaaa agttatgaaa cacaagcttt ctgcaatcct tatggccttc atgttgacga
      781 cgccagctgc gtttgctgcc tctgaagcaa caaacgggac cgaggcaacg acgggtacta
      841 ctggcaccac aacgaccact accggcgcaa ccacgactgc tactaccact ggtggcgtgg
      901 ctgctggcgc tgtcggcacg gcgacagttg tcggtgtggc aacggctgtt ggtgtggcga
      961 cgttggcagt tgttgcagca aatgacagtg gcgatggcgg ttctcataac acctcaacca
     1021 ccacgtccac tacacgctaa tactgcttta acgcgtcgaa attaaagtta aatgataccc
     1081 ggttgcgata ttcgtaccgg gctttagaga tttgcatttt tttaattaac ctttcaagga
     1141 ttaaaggtgc gccctcttat tttatcgatt ttcgcactat ttcttgcggg atgtacgcac
     1201 agccagcaaa gtatggtcga tacatttcgc gccagccttt tcgataatca ggatatcacc
     1261 gtagcggatc agcagatcca ggcgttgcct tattccacga tgtatttacg ccttaatgaa
     1321 gggcaacgaa tctttgtggt actgggatat atagaacaag aacaaagcaa atggttatcc
     1381 caggataacg ccatgctggt tacccacaat ggacgtcttt taaaaaccgt caaacttaat
     1441 aataatctgc tggaagtgac aaattccggg caggaccctc tgcgtaacgc gctggcaata
     1501 aaagatggca gccgctggac gcgcgatatt ctctggagtg aagacaacca ttttcgctct
     1561 gcgaccctga gttctacttt ttcctttgct ggattagaga cgctgaatat tgcgggtcgc
     1621 aatgtgctgt gtaatgtctg gcaggaagag gtgacttcca cgcggccaga aaaacagtgg
     1681 caaaacacat tctgggtcga ttcggctact ggccaggttc gtcaaagtcg gcaaatgtta
     1741 ggcgcagggg ttattcccgt agaaatgacg tttcttaaac ccgcaccatg aataaattac
     1801 agtcgtattt cattgccagc gtactttacg taatgacacc ccatgccttt gcgcaaggaa
     1861 cggtgactat ttatctgcct ggcgaacaac agacactttc cgttggcccc atggaaaatg
     1921 ttgtccagtt ggtgacacaa ccgcaactgc gcgatcgcct ctggtggcct ggtgccttgc
     1981 tgaccgactc tgcggcgaaa gctaaagcgc ttaaagacta ccaacatgtc atggcacagc
     2041 ttgcttcctg ggaagcagaa gctgacgacg atgtggcggc aacgattaaa tccgtgcgcc
     2101 agcagttact caatctcaat atcactggtc gcttaccggt caaactggac cctgattttg
     2161 tacgagtgga tgagaacagc aatcccccgt tggtcgggga ctatacgctc tataccgtac
     2221 agcgtccggt gactattact ctgctgggtg cggtttctgg ggccgggcaa ttgccctggc
     2281 aggcaggccg tagcgttacc gattatctgc aagatcaccc ccgccttgca ggcgcggaca
     2341 agaataacgt tatggtgatt acgcctgagg gtgagactgt cgtcgcccct gttgccctgt
     2401 ggaataaaag acatgtggaa ccaccgcctg gcagccagct ttggttgggc ttttctgcgc
     2461 atgtactgcc cgaaaaatat gctgatttaa atgaccagat tgtgtctgtt ttaacgcaac
     2521 gtgttcctga ctgatgaaga agaattctta tcttttaagc tgcctggcca ttgccgtctc
     2581 cagtgcctgt catgctgaag tattaaccta cccggatccg ctgggttcgt cgcaatcaga
     2641 ctttggcggc acaggattgt tgcagatgcc aaatgcgcgc atcgcaccgg aaggtgaatt
     2701 cagcgtcaac taccgggata acgatcaata ccggttctac tccacctccg tggcgctgtt
     2761 cccatggctg gaaggcacca ttcgttatac ggatgtgcgc acacgcaaat atagccagtg
     2821 ggaagatttc agcggcgatc agtcatacaa agacaaatca ttcgatttta aacttcgcct
     2881 gtgggaagaa ggttactggc taccgcaagt ggcgtttggt aaacgtgata ttgctggtac
     2941 gggtctgttt gacggtgagt atctggtggc cagcaagcaa gcggggccat ttgatttcac
     3001 cctcgggatg gcatggggct acgccggtaa tgcgggcaat attaccaacc cgttttgccg
     3061 ggtgagcgat aaatattgtc atcgcgcaga gtctcacgat gcgggcgata tcagctttag
     3121 cgatatcttt cgtggcccgg cttccatctt tggcggcatt gagtatcaaa cgccgtggaa
     3181 tcccctgcgt ctgaaactcg aatacgatgg caacaattac cagaatgatt tcgctggcaa
     3241 actgcctcag gcaagccatt tcaacgtcgg cgcagtttat cgcgctgcca gctgggcaga
     3301 tctcaacctg agttatgaac gcggtaacac gttgatgttt ggcttcacgt tacggaccaa
     3361 tttcaacgat ctgcgccctg ccctgcgcga tacgccaaaa ccggcatatc aacctgcgcc
     3421 tgaatctgaa ggattgcagt acaccacagt agcgaaccaa cttaccgcgc tgaagtacaa
     3481 cgcaggtttt gaagcaccgg aaattcagct gcgcgataag acgctgtata tgtctggtca
     3541 acaatacaaa taccgtgatt ctcgcgaagc ggtcgatcgt gccaaccgga ttctggtgaa
     3601 taacctgccg caaggcgttg agaagattag cgtgacgcaa aagcgcgagc atatggcgat
     3661 ggtgactacc gaaaccgacg tagccagcct gcgcaaacag ctggctggta cagcgcctgg
     3721 tcaatcagag caacttcaac aacaacgtgt tgaagcagaa gatctttctg cctttggtcg
     3781 gggctaccgt attcgtgaag atcgctttag ctactctttc aacccaacac tttcacagtc
     3841 gctgggcggc ccggaagatt tctatatgtt ccagctgggg ctgatgtcca gtgcccgcta
     3901 ctggtttacc gaccacctgc tgcttgatgg cggtattttc accaatattt acaacaacta
     3961 cgacaagttt aagtcttcgc tgttgcccgc ggactctacc ctgccccgcg tgcgcacgca
     4021 tatccgtgat tacgttcgca atgacgttta tctcaacaac ttgcaggcga actactttgc
     4081 cgacttaggc aatggtttct atggccaggt gtatggcggt tatctggaaa cgatgtacgc
     4141 cggtgtcggt tccgagctgc tttatcgccc gctagatgcc agctgggcgc tgggtgtgga
     4201 cgttaactac gtgaagcaac gtgactggga caacatgatg cgcttcaccg attattccac
     4261 gccaactggt ttcgtgacgg cttactggaa cccgccgacg ctcaatggcg tactgatgaa
     4321 acttagcgtt gggcaatatc tggcaaaaga taaaggggca acgatcgacg tcgccaaacg
     4381 ctttgacagc ggcgtggcgg taggggtatg ggcggcaatc agtaacgtat ctaaagatga
     4441 ctacggcgaa ggcggcttta gtaaaggttt ttatatctcg attccgttcg acttgatgac
     4501 cattggacct aaccgcaacc gcgcggtggt ttcgtggaca ccattgacgc gtgatggtgg
     4561 acaaatgctg tcacgcaaat accagctcta tccaatgacg gcagagcgag aagtaccggt
     4621 tggacaataa cctgacgtaa ttcataacat tacattgact gataaatata aaccaatgaa
     4681 aaagaacatt tttaagtttt cggtactgac attggctgtt ctgtctttga cagcatgtac
     4741 ccttgttcct ggtcagaatt tatcgaccag taacaaggat gtcattgaac tacctgacaa
     4801 tcagtatgac ctggacaaaa tggtcaatat ctatcccgtc acgccaggat tgattgatca
     4861 gttgcgcgcc aaaccgatta tgtcgcaggc taacccggag ctggagcagc aaatcgcaaa
     4921 ctatgaatat cgtatcggga ttggtgatgt gttgatggtt acggtctggg atcacccgga
     4981 actaaccacc cccgcagggc aataccgtag cgccagtgat accggcaact gggttaacgc
     5041 cgatggtgcg attttctacc cctatattgg tcgcctgaaa gtggccggga aaacacttac
     5101 ccaggtacgc aacgagatta cagcgcgtct cgattcagtc attgaaagcc cgcaagtcga
     5161 tgtcagcgtc gcggcgttcc gatcgcaaaa agcgtacgtc accggcgaag tcagcaaatc
     5221 gggtcagcag ccgattacca atattcctct gaccatcatg gatgcgatta acgccgcggg
     5281 cggtctgacc gcagatgcag actggcgcaa cgtggtgctc acccagaatg gcgtaaagac
     5341 caaagtgaac ctctatgcgc tgatgcagcg tggcgacttg cgccagaaca aactgctgca
     5401 cccgggcgat attttattta ttccgcgcaa cgatgatctg aaagtcttcg tgatgggtga
     5461 agtgggtaaa cagagcacgc tgaaaatgga ccgcagcggt atgaccctgg cggaagcgct
     5521 gggtaacgct gagggtatga atcaggatgt cgctgatgcg acggggattt ttgttattcg
     5581 tgcaacgcaa aacaagcaga atggcaaaat cgccaatatt tatcagctga atgcgaagga
     5641 cgcatcggca atgatactcg gaacggaatt ccagcttgaa ccttacgaca ttgtttatgt
     5701 cactaccgcg ccgctggctc gttggaaccg tgtgatttct ctgctggtac cgacgatttc
     5761 cggggtgcat gacctgacag agacttcacg gtggattcaa acatggccca actaaaattt
     5821 aactcaatcc tggtggtttg taccggcaac atttgccgtt cgcccattgg cgagcgcttg
     5881 ctgcgtaaac gtctgccggg cgtaaaagtc aaatcggcag gtgttcatgg tctgataaaa
     5941 caccctgcag atgcgacagc ggcagatgtt gccgctaatc atggcgtatc tcttgaagga
     6001 catgccggac gtaagctcac tgctgagatg gcacgaaatt acgatctgat tctggcaatg
     6061 gagtcggaac atattgctca ggttaccgca attgctcccg aggtccgcgg gaagacaatg
     6121 ctgtttgggc aatggctgga acagaaagag atcccggatc cctatcgtaa aagtcaggac
     6181 gcatttgaac atgtctacgg tatgttggag cgcgccagtc aggaatgggc gaagcgcctc
     6241 agccgataat ttgagtttat aaaatacgat gacaactaaa aatatgaata cgccaccagg
     6301 cagcactcag gaaaatgaga tcgatctgct tcgtctggtc ggcgagttat gggatcaccg
     6361 taagtttatt atcagcgtga ccgcgttatt cacgctgatc gctgtcgctt actcgctgtt
     6421 aagcacacca atttatcagg cagatactct ggtccaggtt gagcaaaaac agggcaacgc
     6481 cattctcagc ggcctgagcg atatgatccc taactcatcg cccgagtctg caccggagat
     6541 ccaactgctg caatcgcgca tgattctcgg taaaaccatt gctgaactga atctgcgcga
     6601 catagttgag cagaagtatt ttccgattgt gggtcgcggc tgggcgagat taaccaaaga
     6661 aaaaccaggt gagctggcga tcagctggat gcatattcca caactgaatg gtcaggatca
     6721 gcaactgaca ctcacggttg gggaaaacgg ccactataca ctggaaggtg aagagttcac
     6781 cgtcaatggt atggtcggcc agcgtctgga aaaagatggc gttgcgctga ctatcgcgga
     6841 cattaaggcc aaaccaggaa cacagtttgt cctgagccag cgtaccgaac tggaagcgat
     6901 taacgcattg caggaaacct ttaccgttag cgaacgcagt aaagaaagcg ggatgctgga
     6961 acttaccatg actggtgatg atccccagtt gattactcgt attctgaaca gcatcgctaa
     7021 caactatttg caacagaata tcgctcgcca ggcggcgcag gattcacaaa gccttgaatt
     7081 cttacagcgc cagttacctg aagtgcgcag cgagctggac caggcggaag aaaaactcaa
     7141 cgtttatcgc cagcagcgcg attcggttga ccttaacctg gaagccaaag ccgttcttga
     7201 gcagattgtg aacgttgata atcaactcaa tgagctgacc ttccgcgagg cagagatctc
     7261 ccagctgtat aagaaagatc acccaactta tcgtgcgctg ctggaaaaac gccagacgct
     7321 ggagcaagaa cgcaaacgcc tgaataagcg ggtatcggca atgccttcca cccaacagga
     7381 agtgttgcgt ttaagtcgtg acgtagaagc gggccgtgcg gtatatctgc aattacttaa
     7441 ccgccagcag gagttgagta tttcgaaatc cagtgccatt ggtaacgtgc ggattatcga
     7501 cccggcagtc actcagccgc agccagtgaa accgaaaaaa gcgttgaatg tggtgcttgg
     7561 ttttattctt ggcctgttta tttctgtggg tgccgtgctg gcgcgtgcga tgttgcgtcg
     7621 tggtgtagaa gccccggaac aactggaaga gcacggcatc agcgtttatg ccactatccc
     7681 aatgtccgag tggctggata aacgcacccg tctgcgtaag aaaaatttat tttctaatca
     7741 gcagcgccat cgtactaaaa atatcccctt cctggcggtg gataacccgg cggattctgc
     7801 tgtggaagcc gtacgtgcgc tacgaaccag tctgcatttc gctatgatgg agacggagaa
     7861 taacattctg atgatcaccg gtgcgacgcc agacagtggt aaaacgtttg tcagttcaac
     7921 tctggcagcg gtgatcgccc agtccgatca aaaagtgtta tttattgatg ccgacttacg
     7981 ccgtggttat tcgcataacc tgtttaccgt gagtaatgaa catggcttgt cggaatatct
     8041 ggcaggtaaa gatgagctca acaaagtgat ccagcatttt ggcaaaggag gctttgatgt
     8101 gattactcgc ggtcaggtgc cacctaaccc gtctgaactg ctgatgcgcg atcggatgcg
     8161 tcaattactg gaatgggcga acgaccatta cgatctggtg attgtcgata cgccgccgat
     8221 gctggcggtg agtgatgccg cggtcgtggg gcgttctgtt ggcaccagcc tgctggttgc
     8281 gcgttttggc ttgaacaccg ccaaagaggt gagtttgtca atgcagcgtc tggaacaggc
     8341 aggcgtcaat attaaaggcg ctatcctcaa tggtgtgatt aaacgcgcca gcaccgctta
     8401 cagttacggc tataactatt acggttatag ttactccgag aaagagtaaa actgaaagtg
     8461 gccggatgcg gcgtgaacgc cttttccggt ctacgcatta gacagttctt cgtttgtcat
     8521 cagcctcaga gcattcaggt aactgaatgc tcttttttat gcattacaaa ctgcacgccg
     8581 gtatgcgtgc ttcattcacg atttgcgtaa aacctgccaa cgaacacatg ccctgcgcat
     8641 ttcgctcttc acatcctgcc agggtcagtt tcacctctcc gggcggcgta tttaatgaca
     8701 gcggcgtttt atcacgcatc tgctgtaaag tctggaagac cagcgaaacc tgaatccact
     8761 ggctgttatc gcttagccga cgccagcgtt caaacaccag ttcaccacct ggcggcgtgt
     8821 tatccggctg accgggaagc gtccagttga gctccagtgc gccgccgaga tttgccagat
     8881 tagtatcgtg tccggcgata aacagcactg aagtgggtaa tgtcacacca tacgcctgtt
     8941 tttgcggtgg atggggcgtc aacgctgtct tgatcaaatc taataacggg gtggcgcggc
     9001 tgcgggcaac ctctggcgtg cgttgtagca aataaaattg cgcgttatgc aaacttagca
     9061 aggtgttcca ctggtgtgaa tcggtgatcc ttccccaccc cggctccggc attccctgtg
     9121 cttgttgcag gagaaatatc tccgtcagca ttgatgcgag gcttaccgca ccggttaatg
     9181 agacattgtc ggcgctcacc ttgagttccg atggtaatgc ctgcgttaat gaacagcttt
     9241 cgtcctgttt ctcacgttta aggcacaagt ttgattgcgg aaaattaagc acccgttcca
     9301 gttcgcgaaa cgccgtttgc cgatgcccgg taaagtcagc aattgaccct cctgccctgc
     9361 tgaggatcgc gtcagtcacg ttcgcgttat ccagttggca aacgccagtt tttagaggat
     9421 taaataacgg atcgggactg gacgtatcag cctgggtatg tacggttatt gcacagtcag
     9481 gtgccagccc ggcggcgaag gcttcgcctg ttttacgggt acgctcgtcg acatcagcaa
     9541 taatcgcgac ctgaccagac tgcgggcagc cctttttcgc cagcaatccg tcggctacca
     9601 gacgctggcg ttggtaatgt ccgagatagg cgattagctc accaccgcgc ggtgtcagcc
     9661 aacccagttt taccggccag gttggccatg cgtctggggt gacatcctgc atcagttgcg
     9721 tggccttggt tggagcacgc acaccatgac gactgacaat caccacactt tccagcttca
     9781 gctccggctc actctgagcg aatgcagatt gcggggttaa cggaatcaga agagataaaa
     9841 atgggattaa gatcgctttc atcgatatgt ttccgctttt catatctgac attattgatt
     9901 gcctgatgcg atgctaatgc gccgcatcag gcctacgttc gttaactttt cagacgcggg
     9961 tccagccata ccaacacaag ggtggagagc gaacacatca acaaaatgcc gacgaaccaa
    10021 agtaaatacc acattgttcc tgctccttag tacaactcgt tttcgttacg gcggagagtt
    10081 tctgttgtca tgcgtcccca cattttgtag tagctccaga gagtgtagag caacacaatg
    10141 ggcaaaaata tcagcacgat taccaacata atgctcagcg tcagctgact ggaagtgctg
    10201 tcccacaacg tcaggctgga gattggactc acgcttgacg gcatgacaaa ggggaacagc
    10261 gtgatgcctg ccgtgaaaat cacgccaaat tgcatcaatg acgccattaa aaatccccaa
    10321 cccgggcgac cacgataaat cgccatcacc gtcagcaatg ggcagaagaa ccccagcagc
    10381 gggaagatcc acaaaacggg cgattcgacg aaattattca tccaggcacc aggtagcact
    10441 gccaccagtt tcattaacgg attggaagga ccgttagcat cctgggcgag cagtacaaag
    10501 ccatcaatac cgacccacag ccagtaaccc gccagcaaaa agcacagcat caccaacagt
    10561 gcagcgcgtt tggtggccag ctgtgaacgc agatgaatca caccaacagt tttcagttgt
    10621 aaccagacgc caccttgcaa aatcaccatc ccaaggctga gcaatccgca caataaaggg
    10681 aatggcgtca gcagttgcca gaagctgccg agatactcca cgcgtaattg cggtgtgaag
    10741 gcaaacggca cgccgagcaa caagttgccg aaggcgatac cgaagactac cggcggcacc
    10801 agactgccga tgaccagacc ggcgtcccac attttacgcc agcgtgcatc ggcgattttt
    10861 ccgcgataat caaaggccag cgggcggaag aacagtgagc acagcaccag gatcatcgcc
    10921 acataaaagc cggaaaacgc cgctgcatac actctgggcc aggcggcaaa taatgcccca
    10981 ccagcgagga tcaaccagac ctggttgcct tcccagtgtg caccaacgct gtttatcact
    11041 atccggcgtt catcatcatt acgcgccacc agcggcagca gacagccgat ccccatgtca
    11101 aatccgtcgg agatcataaa gaccaccagg atcacgccaa tcagcagcca ccagatgaag
    11161 cgcaatgttt cataatcaaa catgattttc ccctttaccc ctgttgctgc gtcggttgtt
    11221 cactctgcat cgcgctcggc cccagacggg catatttctg catcaggtag acttcggcga
    11281 ttaagaacag ggtgtaaagc cctacgatca tgatcagtga gaaagccagt tgtcctgtgg
    11341 ttaaagcgga gtgcgcggag tatgtcggta agatgtcctg tatcgcccac ggctgacgac
    11401 caaactcggt cataaaccac ccggcttcaa tcgcaatcca cggcaacggc aaactccaga
    11461 gcgccatttt cagcacccag cgatgctggt cgattttgcc acgcagcgtc tggacaagcg
    11521 caatcagcat caccagtagc ggcagggaac cacagcccac catgatgcgg aaactccaga
    11581 ataccggcgc aacctgaggt atcgcgccac gcatcgccgc ctggtactgt gcggctgtga
    11641 catgattcat atccggcgca tagcgggaga gcaacatgcc gtagcccagg tcaccttcca
    11701 gttcccggaa tgcctgcaac acatgcggct cacgattgcc ttgcgatatt tcctgcatta
    11761 acagccaggc catacgtccg cgttgcaagc gtgggtaggt ttcagccatc aaatttttca
    11821 gacccggcac ggttttatct aatgagtgag tggcgaggat ccctaacagc gcgggaattt
    11881 tgatggcaaa ggcgttacgc tcttgatcct gttccggcca ggcaaccaca tggaacggtg
    11941 caggagcagg ttccgtttgc cactcccctt ccatcgccgc cagttttacc ggttgtactt
    12001 gcgcgacttc atacgcagaa ctgtctccga gttgtagcgt gccgataatc gccagagtac
    12061 cgaagacgga accgatggca aacgagcgta atgcaacatc gcgctcccgt ccgcgcagta
    12121 aataccaggc gctgatcgcc ataataaaca tggccccggt cacgtagccc gccattacgg
    12181 tgtgcacaaa tttcacctgg ctgaccgggt taaagaccag ctcgctgaag ctggtcatct
    12241 ccatacgtag ggtgtcgata tcaaaatgcg caccggtcgg gtattgcatc caaccgttgg
    12301 cattcaatat ccacaatgca gagagatttg aaccgaacgc caccagccac gtcaccagca
    12361 ggtgctggta tttattcagc cgctgccagc cgaagaagaa aagcccgaca aaggtggatt
    12421 cgaggaagaa ggccattaat gcttccatcg ccagcggtgc gccaaaaata tcgcccacat
    12481 agttggaata gaatgaccag ttagtaccaa actgaaactc catggtcagg ccggtagcca
    12541 cgccaagagc aaagttgata ccgaagagct taccccagaa gcgcgtcata tcgcggtaga
    12601 ttgttttgcc ggtgaccacg taaatggttt ccatgatagc cagcaaaaaa atcagcccca
    12661 gggtaagggg tacaaataaa aagtgataca gcgcggtcag agcaaactgc cagcgcgata
    12721 aatcaatgac atcccacatg cgcactcctg taggcgaaga gagaaaaata acccgataat
    12781 gttagatctc cctctttatc ctgaaactct acgaccagag gtattcgccg attgcgatat
    12841 tcataagact gaaagcgata cttaccgtct ttttacgtcg gtgcagcttc cgccagccac
    12901 tgacatacct cgctaagccg ctgcgcagag tcgaccaaat cttctggcgc tgccagcacc
    12961 acttccggta ttgggcagat ttcataactt tccagtaaag gaccttttaa ggtgtccgta
    13021 cagcgtaaat gccagacatg gcgtaacccc gtggcgttga tatagctctc gccatagcca
    13081 atggtacgaa tctgaatatt tcccggccca cagagacgtg agagaaaaag ccgatcagcc
    13141 tcgctgatgg gtaattgcgt cagattaatg ctgtgcggct gcgcgtcggg gttacgtaca
    13201 tgcgccagta actcatgcgc caacggtagg ccattcatca ggccatcaat gggcggcggt
    13261 aacagcgaat ctgtcggcaa gagattttgc gtggccgcct gccacaacgc cagcggcgcg
    13321 cagccggcct ccagtttgtc ctccagcaac ttttcgccgc gacgtttgcg cacccgccat
    13381 aatccgcaga agatcgcctc ctgtatttca ctttcgctgt cgtcagcctg ctgaatgcgc
    13441 actgacactt ccccttcccc gagaagcgta ttgataaagt gacgatcgtc ggcgttgaga
    13501 tttaacagat cgactgccag cacctcgccc aacggcggct ggcgttgact tagttgatgt
    13561 tgtaactcgt ttaacagcgc aatcacctgc gggctgtgag cacattgctc cagggccgcc
    13621 atactcggtt catcattcac ctgacagctg atcggcagtg gattcatgct gaaactgtca
    13681 tcgttcggtt gcgttcctgg ccccagcaga tggaaaaaag tttcgctcat gaggcacgct
    13741 cctgttgtgg ttcgacaagc ccacgcatca ggtttatcag ttccgctcac gggtgaatac
    13801 cattcagcac accgcgatag tttccgccgg taaacactaa tgtggcggga aagcgaaaga
    13861 cgccaaagcg atctccgatg gcttcgctct gttcaaggtc agcaatcgcc acctgccatg
    13921 tgtagccggg aaactcgcgc agtaattcgc caatcattac cggattatcg ctgacctctg
    13981 gcgtgcgttt cgggtcactg cttaataaca ccacgccgtc tggcgcttgc gtaagccagt
    14041 cgtcaagacg gaattcacta accggcgtcc agccgcgcgc cagcattcgt tgccacaacg
    14101 catcaaatgg cgtgtcgttg ctcatccctg ctcctccagt gtcatcctgt actggcgcaa
    14161 gcgaacgcct tcgtaatttt ccatcgacag acagtcataa ttgagacagc gcgattcatt
    14221 agccggttgc ggcacaattc cccatgcagc cagctgcgcc agcgccgcct gttccgctgc
    14281 gggcagttgc tcccgtgcca gttcgctcag gctgccgccg tagtcgtcga gcattgcggg
    14341 ttgcagaccg acgagggcaa tatgtgctgg cagatgtccg cggatatctg ccagcgccag
    14401 cacttcggag aaactgttct ggtgcaggct cattttcttc gcgctgagat aagccggaat
    14461 acgttctccg gcataggttc gcagcgttcc aggttccagc ccgtagtcaa tggcatcgag
    14521 aatcaacaga tggctggcgc tttcgacata ccccagcaag ttcagtccct gagtaccgcc
    14581 atcgacaatc tccacatact cgggccagtg gtaatgggca tacaaccgtt ccgccacccg
    14641 cacgccgaaa ccttcatcgg cccacagcag gttgcccagc cccattacca caacgcgttg
    14701 ctcgctcatg aacgctcctt gttacttatt ttgccaaatt tgtggctacg gtagccgttg
    14761 accatggtgg agatcaccgt gtcgtcggac atgatgtctt cacgcagcgc catatagaca
    14821 tgaccgatca caaacgcgcc aatcagccac atccccagcc gatgccagct gtgaatgtcc
    14881 attgagttgc ctcccgtcca gtagaaaaat tccaccacat aacggaacgg cgcaaaaata
    14941 gcgtactggc tgtgttcgct gtacagcgca aagccggtga tgatcataaa gaccgacatc
    15001 aggaaatagc cgaacatcgc cgcctgggcg atggggttat ggcctatatc ggcactcgga
    15061 cgttttgcca gaacagatac cagcggattt cataccacac gccctgccac cagcttttac
    15121 gccataccgg cacgataaac agctcgcggg agtatcgatt gccaacaaaa gcccagtaga
    15181 tccgcatcag caagaccacg gtaaaaacca tcccggcgct gaagtgaatt aacctgatgt
    15241 agcccatata gaacagatac gtcgcctcgc cgctgacgga aggtagcggc ttgccgataa
    15301 agtatccggt gaccatcaac accgccatgc ataacaccgt cagccagtgc cagatgcgga
    15361 ctggcgcttc aaagacatag tggctgacaa cgttgtcgct tttctgttgc atgatgattc
    15421 tccttcgctg ttaacgcacc tgcacggaga tcagctcgct accgtcgtcg cccagcacgt
    15481 gtgttgaaca ggcgaggcac gggtcaaagc tgtgcagagt acgcaggatc tccagcggtt
    15541 gctcggggat cgccattttg gtgttcatca gcgccgcttc ataagcgcca atctgccctt
    15601 tgggatcgcg cgggctggtg ttccaggtgg tcggcaccac gcactggtag agatcaatct
    15661 tgccatcgcg aatggcggcc cagtggccta acgccccgcg cggcgcttcg gtaaagccga
    15721 cgccacggca ctctgtcggc caggttgcag gttcccattt ttccgtggag gcggtggcaa
    15781 gattgccgtt tttcaggtta gtcatcagct tgttgaagaa atattgcaac ttacctgcgg
    15841 cccactgtgc ttcgtgcgcg cggcacaaaa tgcggcccaa cgttgactgg ataccggaaa
    15901 gcggcaggtt cagcgccgac atcatgcgat cgaccgactc aacggtcgca gcatcgcctt
    15961 tgtgataagc gattaacgtg cgtgccagcg gccccacttc catcgcgtta ccgcgccagc
    16021 gcggcgcttt gatccacgag tagcgttcct gttcattcag ctgctgaatg ttggtatcgc
    16081 tgcctttgac atcgccgggg ttgtaccacg ggtcggtaat gccatcgaat ggatgacgcc
    16141 cgacctgatc gttgggataa cgataccagg cgtggtcgac aaactcctgc acctgctgcg
    16201 gatcaaccaa atccactggc agcacattgt tgaagtcgcc gttaatcacc gcgccgccag
    16261 gcatcagcag acttttctca ccaaagtcgt tggcaatatc cgggaacgca ccgtagctga
    16321 ggacacattt atcagaaaga cccgtgccga tttcgctcca cggtttgttg aactgaccga
    16381 tggctaaggc gtcggggatc atcacgttgt taatgaagtc cgccgtgcgg gtgataatcg
    16441 actgcaccag gttcaggcgt tccatattga ccgccccgac cgcgccgctt tcgtcaatgt
    16501 taatggcgca aggcatcccg ccgacaatcc agtttggatg cgggttttta ccgccaaaga
    16561 ccgcgtggat tttgacaatt tcacgctgga aatcgagagc ttcgagatag tgggcaaagc
    16621 ccatcaggtt agcttctggc ggcagtttgt actgcgggtg cccccagtag ccattgcgga
    16681 agatccccaa ctgcccgcct tcaacaaatt ttttcaggcg gttttgtacg tcgaagaaat
    16741 agccagggga tgatttcggc caagaggaga gactttgcgc cagttcggag gttttccgcg
    16801 ggtcggcttt cagcgcatct aacacatcga tccagtccat cccggcaagc tgatagaagt
    16861 gcaccagatg atcgtggcac cagagcgttg ccagcataat gttgcggatg atattggcgt
    16921 tgtccggcac tttaataccg atagcatctt cgatggcgta aaccgaagcc agggcgtgta
    16981 cgccagtaca gacgccgcag atacgttcaa cgaacgccca cgcatcgcgc gggtcgcgcc
    17041 cttgtaggat gatctccagc ccgcgaaaca tggtgccgca ggagacggca ttggtgatca
    17101 cattctgatc gttaatattc acttcgcagc gcatgtggcc ttcgatgcgc gtaatcgggt
    17161 cgaccaccag gcggcgtccg gcattattga tggtgtatcc ctgagtttcg tactgagtgc
    17221 tcatgcctgt ttatcctcat tgcctggctg atgttcggtt tctgtaggtt gctggttatg
    17281 acgtctgcgc tggtcaacgg cgctggcgac tgcgtgcaca ccaacagccg ctgccaccac
    17341 accaagcgcg gttaaaccga cggtatcggc ggtggaatga gtacccattt gcggaatatc
    17401 gaccacgcgg ctgtagaacg aaccgcaatc ccagaaacca ttttccgcac agcccaggca
    17461 gccgtgacca gactggattg ggaaagaaac gccatcattc cagcgtgtgg aggaacaggc
    17521 gttataggtg gtaggccctt tgcagcccat tttgtacagg cagtaacctt tgcgggcagc
    17581 gtcatcatcc caactctgga cgaactctcc ggcgtcgaag tgggcgcggc gatagcattt
    17641 atcgtggatt cgctgaccat agaacatcag cggacggccc attctgtcga catctggcaa
    17701 gcgatcaaag gtcaccatgt aagtaatgat ggcgctcatc acatccggga tcggcgggca
    17761 gccaggtact ttgataatgg gtttgtcggt gatgactttg tcgataggcg ttgcctgcgt
    17821 cggattgggc cacgcggcct gcacgcagcc ccaggacgcg caggttcccc aggcgataat
    17881 cgcgctggct ccggcagcgg cacgtttgag tttctcaata aacggtcgac cgctgctgat
    17941 acagaacatc ccctgctcgc ccagcggcgg attaccttct actgcgagga tatatttgcc
    18001 attgtattgc gtgatgatgt cttcgaagac ttcttccgcc tgggttccgg cggcagccat
    18061 caaagtatcg tcataatcga gggaaatcag ggaaaggatg acgtccttcg ccagtgggtg
    18121 agcggagcgg ataaaagatt cggtacagca ggtgcattcc agaccgtgga tccataccac
    18181 cggaatgcgg ggtttgttct ccagcgccca ggcaatcttt ggtgccattc ccgcgcctaa
    18241 tcccagcgac gtggcagcca gactacaata tttgagaaag ctgcgccggg taacgccctg
    18301 acgccgcatg gcctggtaaa atgtttcctc gttattcata tcgcacgtct cttctccttg
    18361 cgacaccggc aggacaccgg gcgcttatgg tcggcaagcg acgtccctgt agcaaggcaa
    18421 accatcgctt cacgtgagat gctgaaaacg aaagctcatc cttctgcact ggcgcacgtc
    18481 gccagaaagt attgttaata aagcgtagtg aaac
//
LOCUS       DAETQN010000083        18211 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.083, whole genome shotgun sequence.
ACCESSION   DAETQN010000083 DAETQN010000000
VERSION     DAETQN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.083"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            15..1091
                     /locus_tag="K0W68_002856"
     CDS             15..1091
                     /locus_tag="K0W68_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HBI3074369.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="K0W68_002857"
     CDS             complement(1484..1825)
                     /locus_tag="K0W68_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBI3074370.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="K0W68_002858"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="K0W68_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HBI3074371.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="K0W68_002859"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="K0W68_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBI3074372.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="K0W68_002860"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="K0W68_002860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBI3074373.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /locus_tag="K0W68_002861"
     CDS             3559..4215
                     /locus_tag="K0W68_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBI3074374.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="K0W68_002862"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="K0W68_002862"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBI3074375.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="K0W68_002863"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="K0W68_002863"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBI3074376.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="K0W68_002864"
     CDS             complement(7167..7826)
                     /locus_tag="K0W68_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBI3074377.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /locus_tag="K0W68_002865"
     CDS             complement(8153..8509)
                     /locus_tag="K0W68_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBI3074378.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="K0W68_002866"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="K0W68_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HBI3074379.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="K0W68_002867"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="K0W68_002867"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBI3074380.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="K0W68_002868"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="K0W68_002868"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBI3074381.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="K0W68_002869"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="K0W68_002869"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBI3074382.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="K0W68_002870"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="K0W68_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBI3074383.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="K0W68_002871"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="K0W68_002871"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBI3074384.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="K0W68_002872"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="K0W68_002872"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBI3074385.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="K0W68_002873"
     CDS             complement(17270..18112)
                     /locus_tag="K0W68_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBI3074386.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4669 a         4575 c         4392 g         4575 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcaggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggctgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat a
//
LOCUS       DAETQN010000084        18151 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.084, whole genome shotgun sequence.
ACCESSION   DAETQN010000084 DAETQN010000000
VERSION     DAETQN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18151
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.084"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..842)
                     /gene="adrA"
                     /locus_tag="K0W68_002874"
                     /gene_synonym="dgcC"
     CDS             complement(<1..842)
                     /gene="adrA"
                     /locus_tag="K0W68_002874"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBI3074387.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(944..1264)
                     /gene="psiF"
                     /locus_tag="K0W68_002875"
     CDS             complement(944..1264)
                     /gene="psiF"
                     /locus_tag="K0W68_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBI3074388.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="K0W68_002876"
     CDS             complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="K0W68_002876"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBI3074389.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="K0W68_002877"
     CDS             complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="K0W68_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBI3074390.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(3341..3544)
                     /locus_tag="K0W68_002878"
     CDS             complement(3341..3544)
                     /locus_tag="K0W68_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074391.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            3622..4716
                     /gene="ddlA"
                     /locus_tag="K0W68_002879"
     CDS             3622..4716
                     /gene="ddlA"
                     /locus_tag="K0W68_002879"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBI3074392.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(4740..4952)
                     /locus_tag="K0W68_002880"
     CDS             complement(4740..4952)
                     /locus_tag="K0W68_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBI3074393.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            5212..5520
                     /locus_tag="K0W68_002881"
     CDS             5212..5520
                     /locus_tag="K0W68_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBI3074394.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(5579..6673)
                     /gene="yaiW"
                     /locus_tag="K0W68_002882"
     CDS             complement(5579..6673)
                     /gene="yaiW"
                     /locus_tag="K0W68_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBI3074395.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6686..7906)
                     /gene="sbmA"
                     /locus_tag="K0W68_002883"
     CDS             complement(6686..7906)
                     /gene="sbmA"
                     /locus_tag="K0W68_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBI3074396.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            8258..9415
                     /gene="ampH"
                     /locus_tag="K0W68_002884"
     CDS             8258..9415
                     /gene="ampH"
                     /locus_tag="K0W68_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBI3074397.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(9416..10039)
                     /gene="iprA"
                     /locus_tag="K0W68_002885"
     CDS             complement(9416..10039)
                     /gene="iprA"
                     /locus_tag="K0W68_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HBI3074398.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(10127..13009)
                     /locus_tag="K0W68_002886"
     CDS             complement(10127..13009)
                     /locus_tag="K0W68_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI3074399.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITNGGVLDVNTNMNDISSVFAT
                     DTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMNSD
                     GHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYIDD
                     TKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQ
                     DTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGNNA
                     KWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSA
                     TMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNDMK
                     VDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTE
                     GSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            13535..14509
                     /gene="hemB"
                     /locus_tag="K0W68_002887"
     CDS             13535..14509
                     /gene="hemB"
                     /locus_tag="K0W68_002887"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBI3074400.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(14615..15466)
                     /gene="tauD"
                     /locus_tag="K0W68_002888"
     CDS             complement(14615..15466)
                     /gene="tauD"
                     /locus_tag="K0W68_002888"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HBI3074401.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(15463..16290)
                     /gene="tauC"
                     /locus_tag="K0W68_002889"
     CDS             complement(15463..16290)
                     /gene="tauC"
                     /locus_tag="K0W68_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBI3074402.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(16287..17054)
                     /gene="tauB"
                     /locus_tag="K0W68_002890"
     CDS             complement(16287..17054)
                     /gene="tauB"
                     /locus_tag="K0W68_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBI3074403.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(17067..18029)
                     /gene="tauA"
                     /locus_tag="K0W68_002891"
     CDS             complement(17067..18029)
                     /gene="tauA"
                     /locus_tag="K0W68_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3074404.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         4522 a         4721 c         4389 g         4519 t
ORIGIN      
        1 ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc gcccacatca
       61 tgaccccagg tatcgttgat gctcttgaaa tggtcgatat cgataatcag taacgttgca
      121 tcgcgattat gccgccgaca gttatcaaat tcattgcgta acatagtttc ccaatgacgt
      181 cggttataca cgcccgtcat gccgtcgcga gtactcatga cctgcaacct gcgtttatgt
      241 tccgccagtt tggttgccgt ctggtagctg acccagccaa acagcagagg ataaacgaca
      301 ataatgggaa gggagagcca ccattccagc ggcgcactat tgaacgacac ggtaatgccc
      361 gtaagctcga gggtgacaag gcaggaaacc accatcaaca ccagacccgc gacaaacaga
      421 cgggggccgc ctgcccccat caaattcaga cacataatca tcaacatagc ggtggaaggc
      481 agcacgttta cgcccattac gcctacccac attcccgcta atactgcatc ggtttttaag
      541 ttgtaaattt cccggctaag cggatcgacg gccctgctcg ctatctgcca ggctaaatgc
      601 ggccagacga acgcccagcc gaccaacacc agccaccacc agcccggcgg cgggtgcgaa
      661 accagcgttg aagcaatcgg taagaacatg ccagccaggc caaccgcacg gggtagtctg
      721 acgcgacggg cgaagcgcag cccggaccgc tgatgttcgt tttgaggagt taaaggaggt
      781 tcctccccat gcgccgccgc ttttttgaaa aagttttcat cattcattat ttttgggaac
      841 attcagaaac aattttccca aattatagag acggggctaa aggcagaagt atgaaatttc
      901 gggtgagcca ctttcgtagc tcacccgtta gcactatcag gtatcaggcg gctttcttga
      961 ggcaggcact cataaactta ttacgatcat cacctttcag agattgttgc gttgcttgat
     1021 tattacattc gcgcatcttt tgctgctgtg gcgtcaaact tttttcacca ggcgcagact
     1081 tgctgttctt caggcaatca ctcatgtagg tcttacgagc atcccctttc aacgcctgcg
     1141 ccgtcgcctg ctgattacag gaggtcatac gctgttgttg tggggttaag gttctctcgg
     1201 cagcgccgac ggtggttaaa aaaaccagac caaaaagcaa ggtaaccagt aatgttattt
     1261 tcatagcacc atccctcttc atgttttaac catgagcgtt tgcgcccgtg atctgccatt
     1321 aagtctggtt gctaacagca aaaaaaccac ccggcagcga aaattcactg ccgggcgcgg
     1381 ttttatttca gccccagagc ggctttcatg gtgtagaaga gatcggtctg gtcggtcagt
     1441 ccaacaacat tggcggcatg cggaccatac gccgcaatac gcaactgact gccggtatgt
     1501 tcttgtgaat cctcttcgga attcccgtaa ctcatcacca tcactgcgcc atctttagta
     1561 tttagcgcct gagtgaggcc cggcgctttg gtatccggcg caacaacctg gctggcgtgg
     1621 gcgtgatcag cggtgactat gaccagcgtg ttaccctcct ttttagcgaa ttccagcgcc
     1681 cgttgtacgg cttcatcgag atcgaccgtc tcgccaattt gcccacaagg attcgcagcg
     1741 tgatcctgtt tatcgattga cgcaccttca acttgcagga aaaatccttt ctcattttta
     1801 ctcaacaatt caatggtttt gtcggtcatc tgtgccaggg ttggtacact gtcattacgt
     1861 tgcggattag gcgtacaggt gactgcgggc ttatcgatat tgccgtggta cgttgctttc
     1921 ggccctagcc agcgcactgg catattgccg tcagcaaaca ggccaagcag gggtttttgc
     1981 tgattcgctt ccgtcaccga attcagtgag gcagcatcgt tcaccaactg ataaccacgc
     2041 gcctgtgcct gttcacgcag cgtttttccc tgccattcac cagcggttgc cgtttcagca
     2101 aaggtttttg cgccgccgcc aagcgtaacg tcggcacgag cgttaagcag ctgttcggta
     2161 atcgatcctt ttccgccttt ttccagagcg ttacccggac atttttcact ggtcgcgctc
     2221 ggaccgtagc atttgcgcga agtcacatgt gccaccagcg cagcgggcgt ggcatcctgc
     2281 aactctgcgg tagaaacgtt accggtcgcc agacctgcgg cttttgccat ttccagaatc
     2341 gttgggtgat ctttttcgtg aatatcgacg cccagcgcgc cgttataggt tttgacaccg
     2401 gttgaccagg cggttgctga tgcagccgag tcggtgacgt agtccggttt gccagttttt
     2461 ttattcagcg catagtgagt gtattgcccg gtaagcggta aggcatcgat acctttaaaa
     2521 aagccgcccg caccttcggc ataattacgt gcggcagtaa tttccgaatc ccccatccca
     2581 tcgccaatca gcaaaataat attttttgca ggtttatcgc taagagaatc acgcagagcg
     2641 gcggtctgat cactcgttaa gcggcgagca ccgccgggtg cagtaatatc gccctgagca
     2701 gcccggtttt ccagaacagg catttctggt gtccgggctt ttgtcacagg ggtaaacagt
     2761 aacggtaaga gtgccagtgc aatagtgctt tgtttcactt tattttctcc atgtacaaat
     2821 acattaaaaa ataaaaacaa agcgactata agtctcggcc gtgacaactt tatgacagct
     2881 gttgaaaaga ttaacttttt actgacgagg atgcttcaat aacttcttta cgtaatcgcg
     2941 cagcagctcc gtatcgtcgt caggaatgct ggcatcgggc tttacctcgt acagcgcccc
     3001 ctctacctga tcaatcaacc gctgttggtc attttgcgcc atattgcgaa gcattgcagt
     3061 gacgataatc tccaaagctt ctacctgcgc acacagttct ttcgactctt cttctttttg
     3121 ggcaagctta aataacaact cagcaatgag atttttcatg tctgtatttc cttatccaaa
     3181 gtatggagaa gttaacatta tgtttcacaa cttcatagtg tctggaagta ttatttttat
     3241 gacaaaggaa atactttgcg caaatatttt ttgtttgatt accagcgaaa cgtttctctt
     3301 atttaaataa ttacccacac aaaatatagg cttttaatta tcaaccttta ttataaataa
     3361 aagttatgaa atatattctc ttcgttaact tataattaag agaaaaaaac tcaccgcatt
     3421 gccatttaag ccaggaataa attagcttcc cgttcatcat ttttgtgatc tcgcgcacat
     3481 ctttgagata acgcactttc ttcaccagta cgttgcacgc tttgtcagca gaagattgca
     3541 acaatctttc agaaggccgc gaatcccccg ttttgtcgtg gtaatgtatg cactttgtgt
     3601 tagacgggat aggtttttaa gatggaaaaa ctgcgggtag gaatcgtttt tggtggtaaa
     3661 tcagcggaac atgaagtgtc tctgcaatcg gcaaaaaaca ttgtcgatgc cattgataaa
     3721 agtcgcttcg acgttgtgct gctgggcatt gataaacaag ggcaatggca cgtcagcgat
     3781 gccagcaatt atctgctaaa tgcagacgat cctgcccata ttgcgttgcg cccttcggcg
     3841 accagccttg cgcaggtgcc aggtaaacat gagcatcagc ttatcgacgc gcaaaacggt
     3901 cagccgttgc cgacggtgga tgtcattttc ccgattgtcc acggtacgct gggcgaagat
     3961 ggttccttgc agggaatgct gcgggtcgcc aatttaccgt ttgtaggttc tgatgttctg
     4021 gcttcagcag cctgtatgga taaagatgtc accaaacgtc tgctacgcga tgccgggctg
     4081 aacattgcgc catttattac cctgacgcgc gctaatcgtc acaacatcag ttttgccgaa
     4141 gtggagtcta aactggggtt accactgttt gtaaaaccgg ctaatcaggg ctcttctgtt
     4201 ggtgtcagca aagtaaccag tgaagaacag tacgcaattg ccgtcgatct ggcgttcgag
     4261 ttcgatcata aagtgatcgt tgagcaaggg atcaaaggtc gtgagatcga atgcgcagtt
     4321 ctgggcaacg acaatccgca agccagcacc tgtggcgaga tcgtactcac cagcgatttc
     4381 tatgcctacg acaccaagta cattgacgaa gatggcgcga aagtggtagt tccggcagcc
     4441 attgcaccag agatcaacga taagatccgg gcgattgccg ttcaggctta tcagacgttg
     4501 ggatgcgcag gcatggcgcg tgtagacgtg tttttaaccc cagagaacga agtggtgatc
     4561 aacgagatca acacactgcc tggcttcacc aacatcagta tgtatccgaa gctgtggcaa
     4621 gccagcggtc tgggttacac cgatctgatc acacgtttga ttgagctggc gctgaagcgt
     4681 cacgctgcgg ataacgcatt gaaagccaca atgtaatatt ttaacgcccg ataatattct
     4741 tattgggcgt tttcagtttc ttcatcgcga cgacgaataa tcaaccccgc cagccagaaa
     4801 ctaatcaccc acgtcaccag accgacggca taggtctgcc aaccttttgc ttcaaatccc
     4861 agtaacccca ccacgccatt aagaatgaat ataaggccaa tggcgaacgc atagtagtgc
     4921 cagtcacggc ggatttttac aggcagattc atggcagctc cgttaacgaa aaacaacatc
     4981 cttgcatatt ctgtaacaac cgtctgtggt gtagacaaca ttttcgtaat tgttaataaa
     5041 tttaacttaa ggttgccatt ttgtaactca caacgaagat tcacgcttcg ggaatattcc
     5101 caacatcaat ttaccatgat tctgatattg acaatctaaa tgacctgtca gacttgccat
     5161 aagccgctgg tatcacacca gaagagaagc agatgacttc cggaggtttt tatggctgat
     5221 ttcaccctgt caaaatcgct gtttagcgga aaatatcgca atgcctcttc aacgcctggc
     5281 aacattgcct atgcgttgtt tgtgctgttt tgcttttggg ctggggcgca attgctgaac
     5341 ctgttagtgc atgcgcccgg cgtctatgag cgtttaatgc aggtccagga aacaggtcgc
     5401 ccacgggtgg aaattggttt aggtgtcggc accattttcg ggctgatccc gtttttagta
     5461 ggctgcctca tttttgcagt ggtggcgcta tggctgcact ggcgacatcg ccgccagtaa
     5521 gatttacgtt gccagatacg gcctaaaacg ccttatctgg catgacattc ctgtttgatc
     5581 actgtttcat acaacgcgcc cgccgttcgt ctacgcgctt cgcaaaccag gccgtagtca
     5641 ggttgcgggt gattttcggg ctttccagtt gaatgccagg caacatttcg cggggtagtg
     5701 atttacccgt tttcgcttcg gcaagttgat aaactttctt gtacagcgcc gtctcctcaa
     5761 aagagaagct gtcacctttc tctaactgac ggcgaatttc gctgtcgctc atccccagtt
     5821 ttcccgccag tttgcgcgtt gccagttcgg ttttcccggg ttctttactg tcgtaacgaa
     5881 ttaaatcacc gtccagtgcc agcttcacgc cgctggcctt actgactgcg ttctgaaacg
     5941 cggcattacg gctggcgtac cagccagcgt taaaatcagc aaagcggtat atcggtgcgc
     6001 tatagctggc gggataattc agtaaatggt aagtaccaaa ccacaacccg ccgcggcgac
     6061 tgaagacttc ctgacgaact gtaccgtcca ttttccacgg ataccctttg gtatgctgtt
     6121 cggcaaaagc aatgctgacc tgcatcggcc caccggtgcg caccggatta agcgaaccaa
     6181 acagcgtctg ccccattggt accatgctga tcagatcgtc aaaaatcgcg ctcaattgct
     6241 tttctgtacg caccgaatct aaccgttcgc tataactttt gccgtttggc gatttgattt
     6301 ttagcgcggt atgaaccaga aaagcaggaa tgtgcatccg ttcagcacga cggtcaatct
     6361 cttgccaggc gatcttgctt aagcccggaa cggccggatc tgcctgataa tttgactcct
     6421 gctgcgccac cgccagcacc gagcagacgt tttccagcgt cggtgccagc ccctgacttt
     6481 caaaggtgat ggcgagatct ttcgcccagg catcgcggtc tttcacgctc gcaggcatct
     6541 tctgccgcac gacgctcgct acatctatcg ctttttcgcc ctttttcaac ggttgcggag
     6601 cctgactact acaccccgct aaaaccagaa ccgccaacaa tgacagcgaa gatagatgat
     6661 tgacgcgcga cattacttct cctttttagc tcaaggtatg ggttacttcc tgaattttgt
     6721 caccatccag ctcatgttca aagctgcgca gacgtttgta gatagacatc aactcaacca
     6781 gtgtggtcca tgagttaatc aggtactgga aagcaccgcg aacctgacca aaaacgttgg
     6841 taatctgcgt catcaggccg agcgtaatcg taccggcaac aattgacgga aacagcaaga
     6901 acaaaccgaa aacgttatcg acctgcaaat agagaatgcg ggcgatgttg aaatacatat
     6961 agtgaaaata gaggcgaaaa tagtttttcc gtacggcgct aaacagctcg cgtaccgtag
     7021 gcggcgtcgc gcgcgtggca tcgtcttcac cataaaccag ctctttacgg taggcggcct
     7081 ctactcgctg gtttttaaac tccagccccg gcagtttgat ccctaccact gccagccatc
     7141 cggtccccat cagcgaccag acgatagcgg caatcaccag accatacgga atgtgcccga
     7201 taatcggcag ctccggcaca tgcgcggaga gcgttaccag caccggcagg aaggcgatca
     7261 acgtcatgat ggcgttgata aaactgacgc ccatattctc cagcgttgaa gcaaaacgca
     7321 tggtgtcttc ctgcacacgc tgtgcggcac cttcgatatg acgcagttgt tgccagttcg
     7381 ccatgtaata ttcgttcatc gccgtacgcc agcggaacac atagtgactg acaaagaagt
     7441 tgttcagtac actgatcacc accgcgatca gcgcaatccc cagaaagacg cccacttcgc
     7501 ggtaaaattg ttcgatagtg actttatgcg gcgaacttag cgcggtttga atcagatcat
     7561 agaacggtgc ataccaggcg ttgacggcga ccccgacttc caccaaaaac caggtgacga
     7621 agatgatcag tgcagtaccg agtattgacc agtattgcca acggtgcggg ctgtagataa
     7681 accagaaaaa tgcaaaaagt cctacgcaaa caatgtagta agcgtaaaaa atcaggaaat
     7741 ccaacgacca gaaacgcgcg gcgctaatcg ggatctgccc ggaagcgccg gtgatacgcg
     7801 ccacccagtc tcccccaccg gcttgccaga agataacggc aatcaatgcc caaacaaagg
     7861 ccgagagaaa aaacgctccc ggctttggga aaaaagactt aaacatatgc actcctgcta
     7921 acttcttatc gttttaacaa ctgctgtgta ttaaccgcat gaccactaca aagcgtcagc
     7981 caaatgaaac cgcccattgt gacccatagc aaggcgctta gttcgccagc ctgaccataa
     8041 gaattgtttc gacggatttc acaaaccaaa gattgtttac ataagcggat taacacgcgc
     8101 aggatcacaa atgcaggcat tggtgagcag gaaaaagatc gtgtttacgc gccgttacgt
     8161 ttttaacact attattgcac taattcgccc tttgcaatcc atcaatgagt agtataaata
     8221 cgctcagtta ccttcattca atctatggac accaccgttg aaacgtagtc tgcttttttc
     8281 tgccgtgctg tgtgcggcgt cattgacctc cgtccatgcg gcgcagccga tcactgaacc
     8341 ggagtttgcc tctgatattg tcgatcgtta tgccgatcat attttttacg gcagcggtgc
     8401 aacggggatg gcgctggttg ttatcgacgg taaccagcgc gtctttcgta gttatggcga
     8461 aacgcgacct ggtaataacg ttcgcccgca gctggattcg gtcgtgcgta ttgcttccct
     8521 caccaaacta atgaccagtg aaatgctggt gaaattgctc gaccagggga ccgtaaagtt
     8581 aaacgatccg ctaagtaaat atgctccgcc aggcgcacgc gtgccgacct acaatgggac
     8641 gcccattacc ctagtgaatc tggcaaccca taccagcgcc ctgccccgtg aacagcccgg
     8701 tggcgcggca catcgtccgg tgtttgtctg gccaacgcgc gagcaacgct ggaaatacct
     8761 ttctacggcg aagctgaaag ccgcgccagg cagtcaggcg gcgtactcta accttgcgtt
     8821 cgacctgctg gcagatgcac tggcgaatgc gtccggcaag ccttataccc agttgtttga
     8881 agagcagatt acccgtccat tggggatgaa agacaccacc tacaccccct caccggatca
     8941 gtgccgccgt ttaatggttg ctgagcgcgg tgccagtccg tgcaataaca cgctggcggc
     9001 aattggcagc ggcggcgtct attccacgcc tggcgatatg atgcgctgga tgcagcagta
     9061 tttgtcatct gatttttatc agcgcagtaa ccaggcagat cgcatgcaga cgttgattta
     9121 tcagcgcgcg cagtttacga aagtgattgg catggatgtc cccggcaaag cggatgcgct
     9181 tggtttaggc tgggtatata tggccccgaa agagggtcgt ccggggatta ttcagaagac
     9241 aggcggtggt ggcggtttca ttacctatat ggcgatgatc ccgcagaaga atattggcgc
     9301 gtttgtggtg gtcactcgtt cgccgttgac gcgctttaaa aatatgagcg atggcatcaa
     9361 tgatctggtg accgagctaa gcgggaataa accgttggtt atccccgcat cctgattaat
     9421 actctgaagg caaacggttg atagcgacca gtttgccttt attcatttcg atatagccgc
     9481 ctttacgcaa agcagcgaga acttctgcga cgacagaacg cgatatgcgt gtacgttgat
     9541 ggatatagtt catcacacca atacgtgatc gcaattcttc attccagtca atcattgata
     9601 ataatgttgc gcggatttgt tcgtaggaat tatgcccaat gagctgcacg tcgcgtaatt
     9661 ccagaattct attttgccaa gctaaccagt aaaaagcgtc tcgccagagt tgattttgtt
     9721 caataagcgt aatggtttgt ttggctggta gatgatatcc cgtacaattt ccttctgata
     9781 ttaatttgta tggtatatcg tttttcatta aaccatcagc cagccccata atataagggg
     9841 cctgggtaat accgataagt acgttttctt ctctacgcag agagataact ccctctaaaa
     9901 taacgaaagt atcttcgtta ttgacatcac tggaaaatat aacttgcttt tcattattaa
     9961 actcgaagcg cgtaccgtat ctggacaaac atttatcgag cttaccaaat tcctgaagag
    10021 gtttaactac agataacatt tgcgcgtcct ttgcagtaat gcccgtcaaa tccttgacgg
    10081 gcattattta gattaaatta ccagtatttc ttcggagtga agaatattac caggtatatt
    10141 taacacccac gttcgcggac cagtcttgat ctacgtcacc accaccgagg tagttagcat
    10201 cggtataggc gctgaagttc ttggtgaagc tgaactgagt acccagacca acacgtaccg
    10261 cagacccttc agtaccgtta tcgatggaat caccgttcac atcgttatcg ttgttagagt
    10321 cgtcgtagac gtaagccagt ttgaagtacg gagtcagagc ctgatcttcg ctgtaggtga
    10381 aggtataacc tgcatctaca cccagttcat aacgcatgct gtcgtaagac tgaccgtcaa
    10441 ctttcatgtc gttgctcagc tggtagtcat caccagactg gaacagacca gaaacgctgc
    10501 cgtaaggagt cacgtaacca gcatcaccca gtttgaagtc gtaaccggct ttcaagccga
    10561 agccccaagc gtcggagttg gtgctaccgt caacgtaagt accgttgctc atggttgcag
    10621 acaggtcgtt gttgaagtga gagtagctca agctaccatc aacaaagacg ttgttcgcga
    10681 agtgagcaga agagtagatg taggcagtct ggctgtcttg atctacctga ccagaacggt
    10741 cattcatgtc acctttagcg aagcctgcag ccgcaccgac gatccactta gcgttgttac
    10801 cgtcaacttt ggtatcaaca ccgaccatga tgccgttaac atcctgatca tagttgatgg
    10861 tgccgttgtc gccgttgaag ttaccaccga agtagcttac ccatgcgccg ccgttatcag
    10921 ccaggccatg acgagagttg gtcagacgag taccaacggt gtcttgttcc aggttccaga
    10981 tattggtgtt cgcagacggg atgctcagcg ccatgttagc gtagtcggtc agctccatct
    11041 gttgcagaac aacggtgtta ccgcgctgtt cagcctgata ggtgtatgca cccaggtcag
    11101 ctttgttagc agcagagaag gtcgcgttgg ttttggtatc atcgatatag atgagctgct
    11161 tacctgcgta atcagcaatc gcaccagcgc cagtggagtt gtcgatacga actttgtagt
    11221 taccggttgc agctacaacg ttgtctgcag agctgttgtc aacttcgtcg ccattgttaa
    11281 tgtcgttgtt accattaacg gtcaagtgac catcagagtt catcgcaata acaccataac
    11341 cgtagttagc tttgctggtg tcgttggtac gatcgttgac gaggtctgca ttcaggatgt
    11401 aatcattact atgaatgtca aacacacctg cgtaaacatt accgttgtta tctttaacgt
    11461 tgccactggt cagttccaga gtatcagttg caaatacgct ggagatgtca ttcatgttgg
    11521 tgtttacatc cagcacacca ccgttagtaa tggtattggc atacagcggt gcatatgccc
    11581 aagctgaagc agtatcttca ccgaagttaa cggttgagaa ggtatcaatg gtcaggtgat
    11641 cggtgttaac ttcaccgtta acttccattg aagaaccacc gttcagagtg atggtgtcag
    11701 aagtcagaga agaatctgca ctcacgttaa cctgagaacc gctgttgatc gccagggtat
    11761 cgatcaggga agttttcgtg gtttcccaat cagaaccgtt gttcagaaca acatcgaata
    11821 cgccgctctg gaatgcgtta gcgttatcaa cacgattcca gccatcttca gagtaagaag
    11881 cgtacgggtt caggctgttt ggtgcaacgt cgtagaaggc ttcgtcagca acaacaacgt
    11941 cataatcaat attcgctgaa ccaacccatt tggtgttgtt cagagtcagg ttcaggtgat
    12001 cgacgttctg atcgtcagtt gcccaaccgc catgagtgta agcagtggta gagttgtcaa
    12061 tcaggtgacc cgtcgcgtca aagttagcgt tccaggtact gtcaaataca acgtcaccaa
    12121 gcaactgaga gttgttgaac gtaacgttgt tgaccatacg ataatcagaa gtgtcatcat
    12181 cgatgaaggc taacgcaaca tcatcagcag gagatgcgcc attgccgtag tcacttggtt
    12241 caacagaatt gccgaaatga ccacgatctg attgttcgtc ttcagtccat gaaccagaag
    12301 ttacagtaga gtcattaaca acgatgttgt tgtcccactg atgaccttcg gtattgtagg
    12361 tcgcattacc gaggtcgtgt tgaaccaaag taatacctgc aacatgggag ttgttgctga
    12421 tgttgatgtt actttcaaca tccagagtca cagcggtacc aagaccagcg gttaccaaac
    12481 gatcataatc tgtcttagat gttttaccgt ctgcatccag ataagtatcg gtgaagtaga
    12541 aggcgtcaaa atcatcgtca atagtagaat tagcgatgtt cagagtgaag atgtcgccat
    12601 caacccagtc gtggccagta taatcgctac cgttggtcca gacgtagtcg ccataaccaa
    12661 cctggtaaga tgtaaccata ccatgaacaa ttgagttgga gatgttcagt gtattaccgg
    12721 tatctgtgcc ataatcgtgg ttcagataat aagtagaaat tacaccattt acagtgtcgc
    12781 cgctgaaagt ggtatcaaag taaccatcag ctgcgtggtc atatacataa ccaccgtaag
    12841 tcagataatc gtgatcaatt ttagtgctgt caccccaatt gtagatggtg gctgcgtcat
    12901 cgtgataccc gaaggtgtta taagtagctg cattagcctg agaggtgata gccagagtgc
    12961 aagccaatgc taattgtgat actacaagtt tctttttcca ggagtgcatt tgtcatccct
    13021 cctcagggac gtaataagtt gatccatcaa ttatcaatgc atagaaaaca tattatgttt
    13081 cgcgctaacg attatgctgc gttacagacg aatggttcaa ttattctttc ccctaagtcc
    13141 gaatgtggac atatataatt tatggagaaa aacacgcatc aaattgataa aaggacgata
    13201 tttcaacatg ttgcagataa tgttattttt gatacatacg cgttataaat atcaaaaaca
    13261 caaaccagat aaatatctta tcaatataca acaaagtttg aattattcaa tttatagcag
    13321 cacaaccaaa cgccaaaaaa aataataacc aagagctata aataaagatt aaaaatattt
    13381 tcagtgaata tgaaattatt gctatttcta ttacaaagat gtcctggtct ctctttcatt
    13441 ctcttcttgt caacatcacg acaactttcg taaaacactc ctgccccgct tcaggtatac
    13501 tgtgcacctt aattccacaa acatcaggca gaccatgaca gacttaatcc aacgccctcg
    13561 tcgcctacgc aaatctcctg cgctgcgcgc tatgtttgaa gagacaacac ttagccttaa
    13621 cgacctggtg ttgccgatct ttgttgaaga agaaattgac gactacaaag ccgttgaagc
    13681 catgccaggc gtgatgcgca ttccagagaa acatctggca cgcgaaattg aacgcatcgc
    13741 caacgccggt atccgttccg taatgacttt tggcatctct caccataccg atgaaaccgg
    13801 cagcgacgcc tggcgggaag atggactggt ggcgcgtatg tcgcgcatct gcaagcagac
    13861 tgtgccagaa atgatcgtca tgtcagacac ttgtttctgt gaatacactt ctcacggtca
    13921 ctgcggtgtg ctgtgcgagc atggcgtcga caacgacgcg actctggaaa atttaggcaa
    13981 gcaagccgtg gttgcagctg ctgcaggcgc agacttcatc gccccttccg ccgcgatgga
    14041 cggccaggta caggcgattc gtcaggcgct ggacgctgca ggctttaaag atacggcgat
    14101 tatgtcgtat tcgaccaagt tcgcctcttc cttttatggc ccgttccgtg aagctgccgg
    14161 aagcgcatta aaaggcgacc gcaaaagcta tcagatgaac ccaatgaacc gtcgcgaggc
    14221 gattcgtgaa tcactgctgg atgaagccca gggcgcagac tgcctgatgg ttaaacctgc
    14281 tggagcgtac ctcgacatcg tgcgtgagct gcgtgaacgt actgaattgc cgattggcgc
    14341 gtatcaggtg agcggtgagt acgcgatgat taagttcgcc gcgctggcgg gtgctataga
    14401 tgaagagaaa gtcgtgctcg aaagcttagg ttcaattaag cgtgcgggtg cggatctgat
    14461 tttcagctac tttgcgctgg atttggctga gaagaagatt ctgcgttaat ttttttcctc
    14521 tcagaaaggg ggagttaacc tgttgaaagt acggtgaacg ccccctcacc ctaaccctct
    14581 ccccaagggg gcgagggaac cgtccgcact cgtatcaccc cgcccgataa aacggtttat
    14641 ccccaaggat cgtcgcccga tgcataatcc gccgctgtgg caggtaatcg gcattggcat
    14701 agtgctgagt cacgcggtta tcccaaatcg caatatcatt cggttgccag cgccaacgca
    14761 cctgaaactc cggtttagtg atatgggcga ataaaaagcc taacaaggct tcgctctctt
    14821 tctcgctgac atcgacaatt cgcgtagtaa agccttcatt cacaaacagc gcctgtttac
    14881 cgctcaccgg atgcgttcgc accaccggat gtagcaacgg aggatttttc gcgaccgcct
    14941 cgcgccagcg ttgatgttcc tcctcggttt tgcggtattt gtattccggg aacgatttac
    15001 ggaaatcatg ctccgcacgc agcccgctca gcagctggcg gaagggaaca gagagcgcct
    15061 cataggccgc aataccgctg gtccagagcg tatcaccgcc ggtcgaaggt aactctttag
    15121 ctgccagaat cgcccctgcg ggtggcgttt caataaatgt cacatcggta tgccagttgt
    15181 cgttatccgg cggattatcg ttatgggtat ccagcacgat gatctcgtca accccttcgg
    15241 catgagggta aacagggtga atatgcagtt cgccaaaacg ctgggccagc gcgcgttgct
    15301 gctgcggcgt aatagcttga tcgcgcagaa ataccacctg atagcgcagc accgcatggt
    15361 aaagctgttc aaactgatta tcgcttaacg ggcgcgtcag gtcggcaccc gaaatttgtg
    15421 cgccgatata cggccccagc ggggtaatac tcagacgttc actcattgta cttctccatg
    15481 ccagggcgtc agacggcgct gtaacgcgcg cagacccagt tctaaaagaa aggcgataat
    15541 cgcaatcacc gcgatccccg ccagtaccac gtcagtcgcg agaaattcac ccgctgactg
    15601 aaccataaat cctaaaccgc gtgtcgcggc aatcagctcc gccgctacca gcgtagacca
    15661 gcccacaccc agaccaatgc gtaatccggt gaggatttcc ggcagcgcgc cgggcaaaat
    15721 gacaaaccac agcacctgcg cacggctggc acccagcgac tgggcggcac gaatgcgaac
    15781 ctgctgcgcg cttttcaccc ccgccagcac cgacatcgcc accggtgcaa aaatcgctaa
    15841 atagatcaat aagatcttcg aggtttcacc aataccaaac cagatcacca tcagcggcaa
    15901 ataagccagc ggcggcaccg gacgataaag ttcgattacc ggatccagaa tgccgcgcac
    15961 cgtcgggctt agccccatgg cgatcccgac cggaataccg atcaccaccg ccgcgagtag
    16021 cgccagcaca atgcgcgtca ggctggctgc cagatgctgc cacagcgtgg cgtccataaa
    16081 tccctgcggt ccggcaatgg tgagcagttt tgccagtact tgttgcggcg gcggcaaaaa
    16141 cagcgggcta atcagttgca gcgccgccac cgtccaccat acagtgagta aaaccgctaa
    16201 cgtgccaatg cttaaggtca cctgacgcga gagcggccag cgccatttca gccgccgcga
    16261 atgcagtttt tcattaatga gcacactcat gagaacgcct cccgttgctc aaatacgcgg
    16321 cttaaaacat attcgcgcat ggcgatgaat tgtggatcgg acttgatgct gcggctcgac
    16381 tctcccgcaa caaagcggcg agcaaagttg agcggcagcc gctccagcac acggccaggg
    16441 ccggatgaaa gcagaaccag ttcagtcgcc ataaacaccg cttcttctat atcgtgggta
    16501 atcaacagca cctgcttgcc cgtctcctgc cagagtttca gcagcagagt ttgcatctgg
    16561 tcgcgggtga aggcgtccag cgcaccaaac ggttcgtcga gtaataacag ctggggattc
    16621 gccgccagcg cgcgggcaat ccccacccgc tgacgttgac caccggaaag ctgccagata
    16681 tagcgttttt ctgcgccttc cagccccact tttttcagca tctggtgcgc gatttccagt
    16741 cgctgcattt tctctatacc tgccaattgc aggccgaacg ccacgttgtc ctgtacattg
    16801 cgccacggta gtagcccttc attctgaaaa accaccccgc gctctgctcc cggtccctca
    16861 atacgcttac ccgccagttg aatgctgcca tgctgataag gcacaaaacc ggcaatcaga
    16921 ttcagcaggg tggttttacc gcagccggac ggccccagca ccaccagtag ctcgccgctt
    16981 tccagcgtca ggttgatatc ttccagtgcc ggtttgccgc cataatcggc atagagatga
    17041 gagatttgca gcatctgcgc ctccttttat tgcacgaagc gcgaggtaac gtactggctg
    17101 taatcattcg ctacagccgg gaccttgccc tgctctttca aaaactgcgc ggtgtcgatg
    17161 attgctttgt tcaccggtcc ggtcagttct gccgtttgtt gctgcggcgt cagataggta
    17221 ttccccttca ccagccccgg aacgtcacct tccggcacgc cgcttaaacg cgccagtttg
    17281 ctgatgtttt ccggctgttt cagccacgcg tctgggttag caatgtacgg ttgctgagca
    17341 tcgatggcgc ttttagcgaa cgctttcacg acctcaggat gtttctcggc aaaatctttg
    17401 cgcaccaccc agacgtccag cgttggcgca ccccactgcc cgacctgttc agaatcggtc
    17461 aacaccttgc cgtctttttc cagggcgtta accgccggtg cccagacata agcaccatca
    17521 atatcacccc gctgccatgc ggcgataatc gcgggcggct gcaggttcac aatctccact
    17581 tgccctggtt taatccccca gtgtttcagc gccgccagca ggctgtagtg ggtggtggag
    17641 ataaacggta cggcaatgcg tttgccaatc aaatcttcgg gtttgctgat agttttcttt
    17701 accaccagcg cttcggagtt acccagtttt gacgccagca agaagacttc aatcggcacc
    17761 tgttggctgg ctgcaaccgc taatgggctg gaaccgaggt tgccgatttg cacgtcgcca
    17821 gaagccagcg cccgcacgat gctggctccg ctgtcaaact tacgccagtc cacgctcgcc
    17881 ccgctctctt tagcaaaggt gttgtcggcc tgagccactt tcgccggttc ggctgaggtt
    17941 tgatacgcca cggtgacgtt caccgcctgt gcctgaaaag cgatgaatgc cagtgcggca
    18001 agaagtgtgt tacgcgatga aattgccatg attgtctgct cccctgtctt gttatgggag
    18061 cagtattcag gaataaaaac attcattaaa agaattagtc gttatcgcac agatgatttt
    18121 attcttagca aaaaaacggc aaaggaaaga t
//
LOCUS       DAETQN010000085        17962 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.085, whole genome shotgun sequence.
ACCESSION   DAETQN010000085 DAETQN010000000
VERSION     DAETQN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.085"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="K0W68_002892"
     CDS             complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="K0W68_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBI3074405.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1598..4654
                     /locus_tag="K0W68_002893"
     CDS             1598..4654
                     /locus_tag="K0W68_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBI3074406.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4651..5061
                     /gene="menI"
                     /locus_tag="K0W68_002894"
     CDS             4651..5061
                     /gene="menI"
                     /locus_tag="K0W68_002894"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBI3074407.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5161..5349
                     /locus_tag="K0W68_002895"
     CDS             5161..5349
                     /locus_tag="K0W68_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBI3074408.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5897..6265
                     /gene="sufA"
                     /locus_tag="K0W68_002896"
     CDS             5897..6265
                     /gene="sufA"
                     /locus_tag="K0W68_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBI3074409.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6274..7761
                     /gene="sufB"
                     /locus_tag="K0W68_002897"
     CDS             6274..7761
                     /gene="sufB"
                     /locus_tag="K0W68_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBI3074410.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASELGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7771..8517
                     /gene="sufC"
                     /locus_tag="K0W68_002898"
     CDS             7771..8517
                     /gene="sufC"
                     /locus_tag="K0W68_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBI3074411.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8492..9763
                     /gene="sufD"
                     /locus_tag="K0W68_002899"
     CDS             8492..9763
                     /gene="sufD"
                     /locus_tag="K0W68_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBI3074412.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9760..10980
                     /gene="sufS"
                     /locus_tag="K0W68_002900"
     CDS             9760..10980
                     /gene="sufS"
                     /locus_tag="K0W68_002900"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBI3074413.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10993..11409
                     /gene="sufE"
                     /locus_tag="K0W68_002901"
     CDS             10993..11409
                     /gene="sufE"
                     /locus_tag="K0W68_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBI3074414.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11558..12562
                     /gene="ldtE"
                     /locus_tag="K0W68_002902"
     CDS             11558..12562
                     /gene="ldtE"
                     /locus_tag="K0W68_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBI3074415.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="K0W68_002903"
     CDS             complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="K0W68_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBI3074416.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="K0W68_002904"
     CDS             complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="K0W68_002904"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBI3074417.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            15140..15349
                     /gene="fumD"
                     /locus_tag="K0W68_002905"
     CDS             15140..15349
                     /gene="fumD"
                     /locus_tag="K0W68_002905"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBI3074418.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            15805..16431
                     /locus_tag="K0W68_002906"
     CDS             15805..16431
                     /locus_tag="K0W68_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBI3074419.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            16452..>17962
                     /locus_tag="K0W68_002907"
     CDS             16452..>17962
                     /locus_tag="K0W68_002907"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HBI3074420.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINF"
BASE COUNT         4588 a         4255 c         4724 g         4395 t
ORIGIN      
        1 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcggtg
       61 attaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
      121 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
      181 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
      241 cccgaaagca atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc
      301 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
      361 cactaacaat accgttcccc aggtggtatc gccggtccag tagagccaga taatcgccgg
      421 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
      481 caacgttgca taaggtacgc cggatacggc gaggccgata ctgccaagca ctgcctgtac
      541 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac
      601 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
      661 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
      721 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
      781 gcggactttc gccatgatcg ccgtaccccc catatccagc aagttgtgcc agcctgcata
      841 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
      901 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
      961 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     1021 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     1081 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     1141 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     1201 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     1261 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     1321 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     1381 cgatttactc gtcgtatttc aagttgcatg cgctgcggct acgttcgttc accccagtca
     1441 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     1501 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     1561 cgacctacat cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc
     1621 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     1681 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     1741 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     1801 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac
     1861 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     1921 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     1981 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcaccag aactttcgac
     2041 cagcaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc agggatcgct
     2101 ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga
     2161 tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta aaaccaatac
     2221 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtctgcaac gccagttaat
     2281 catcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     2341 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     2401 ggcctttatt accgaagcgc ggctggatat tacgcccttg cctaaagtgc gccgtctggt
     2461 gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta tggttgaggc
     2521 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     2581 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcgggct
     2641 gaacattgtg gaatttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgcact
     2701 ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt
     2761 gtgccgcgag ctggcggggg ttgaacgtat ctatgcaatg cgcaaaaaag ccgttggtct
     2821 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     2881 gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     2941 aagctacggt atgttcggtc acgtcgacgc aggtgttttg cacgtccgtc cggcactgga
     3001 tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct
     3061 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     3121 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     3181 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     3241 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     3301 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     3361 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     3421 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     3481 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttacggacgt taattgcccg
     3541 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     3601 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc cagtgcccca tcaaaattga
     3661 tgtgccggag tttcgctctc gtttcctgca gctctatcac acccgttatt tacgcccgct
     3721 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa
     3781 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     3841 catggttgat ttgccactgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     3901 ctcggcaaac atgacgctgg aacagcttga agctctcaat gcagagcaga aagcgcgcac
     3961 ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     4021 tgtccgtctg gttgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     4081 caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga aaaagacggc
     4141 ggatttcctc aaccgtatgg ctaagcttgg gatgccaatg gtgggcgtcg atccggcgct
     4201 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa
     4261 cgtcttactt gcgaatgaat ggctggcaag cgcacttgag tcacatccag tggctacagt
     4321 cagcggtgaa tcatggtatt tctttggtca ctgtaccgag gttaccgctt tgccgggcgc
     4381 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     4441 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct
     4501 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     4561 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     4621 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     4681 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     4741 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     4801 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
     4861 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc
     4921 cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt
     4981 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
     5041 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt
     5101 cattatctgc acagtaaacg gtggtatact gtgcacgtct tgtctaaaag cgaggatgct
     5161 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
     5221 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
     5281 ctgcttactc tctcttctac tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
     5341 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
     5401 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
     5461 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     5521 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     5581 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
     5641 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
     5701 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
     5761 aatcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
     5821 gtttgctggg ttgaactgat aatcattatc actagcatgc tgttatacgc tgaaagcgat
     5881 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
     5941 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
     6001 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
     6061 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
     6121 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
     6181 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
     6241 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
     6301 gatgtcaaaa cctggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
     6361 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
     6421 cgtaatgagc cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag
     6481 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagt
     6541 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acttggcgcg
     6601 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
     6661 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt
     6721 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
     6781 ggtgaggcga tccacgatca tccggaactg gtgcgtaaat atctcggcac tgtggtgccg
     6841 ggaaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
     6901 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
     6961 gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
     7021 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcggtggtg
     7081 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
     7141 ggcgataaca acaccggcgg tattctcaat ttcgtcacca agcgtgcttt gtgcgaaggc
     7201 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat
     7261 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
     7321 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
     7381 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
     7441 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
     7501 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
     7561 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
     7621 caacgcggga ttagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
     7681 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
     7741 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
     7801 gtggaagata aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac
     7861 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcga
     7921 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcgctg
     7981 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
     8041 cccggcgtca gtaaccagtt tttcctgcaa acggcactta atgcggtgcg cagctatcgc
     8101 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
     8161 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
     8221 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
     8281 gaatcggact ccgggctgga tattgacgca ttaaaagtgg ttgccgatgg cgtgaactcg
     8341 ctgcgtgacg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
     8401 atcaagcctg attacgtcca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc
     8461 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc
     8521 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
     8581 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
     8641 atatacgccg ctggaagggc tgaccaatag tcagtttgtc agcattgcgg gagatatatc
     8701 cccacagcag cgtgatgcct tagcgttaac gttagacgcc gtgcggctgg tgtttgtcga
     8761 tggacgttac gtgcccgcgc tgagcgatgc aattgaaggc agcggatatg aagtgagcat
     8821 taacgacgac cgtcaggggt tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
     8881 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
     8941 gccattgctg ttaatgcata tcacccaggg cgtggcaggt gaagaggtga acactgccca
     9001 ttaccggcat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgt
     9061 cagccttaat gatgctcgtc attttaccgg ggcacggttc actatcaatg tcgcagcgaa
     9121 cgcccacttg cagcatatca agctggcgtt tgaaaacccg ctcagtcacc actttgctca
     9181 taacgatttg ttgctggctg aggatgccac cgcatttagc catagtttcc tgctgggtgg
     9241 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
     9301 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
     9361 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
     9421 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
     9481 gaccaacaat aatctgctga tgggcaaaat ggcggaagtg gatacaaaac cgcagctgga
     9541 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
     9601 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
     9661 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
     9721 gctggcccga atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa
     9781 gtgcgggccg actttccggt gctttctcgt gaggtgaacg gtttgccgct ggcttatctc
     9841 gatagcgccg ccagtgcgca gaaaccaagc caggtgattg acgccgaggc tgagttttat
     9901 cgtcacggct acgcggcggt gcatcgcggt attcatacct taagcgccca ggcgaccgag
     9961 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    10021 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ttggggcaac
    10081 agcaacgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    10141 gttccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat ccctctcaac
    10201 cccgacggta cgctgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg
    10261 ctggcaatta ctcatgtctc caacgtgctg ggaacagaaa atccgctggc ggaaataatc
    10321 acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    10381 catccggtgg atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg
    10441 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    10501 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg
    10561 accaaagcac catggcggtt tgaagccggt acacccaata ccgggggcat tattggtctt
    10621 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata acatagccga gtatgaacag
    10681 aatctgatgc attatgcgct atcacagctg gaatctgtac cggatctcac tctctatggc
    10741 ccacaaaaca ggcttggcgt tattgctttt aatctcggta aacaccacgc ctatgatgtt
    10801 ggcagttttc tcgataatta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    10861 ttaatggcct attacaacgt ccctgcgatg tgtcgggcgt cgctggccat gtataacacc
    10921 catgaagaag tggatcgtct ggtgaccggc ctgcaacgta ttcaccgttt gctgggataa
    10981 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    11041 tgcgccaact gggaagagaa atatctctac attattgagc tgggccagcg tctgactgaa
    11101 ttacgcgacg aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    11161 attgtcatgc gccagaatgc gcacggaatt attgaattgc agggcgacag cgatgcggcg
    11221 attgtgaaag ggcttattgc ggtagtcttt attctctacg atcagatgac gccgcaggat
    11281 attgtcagtt tcgatgtgcg tccgtggttt gaaaaaatgg cactcaccca acatctcacc
    11341 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    11401 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    11461 tttacactga cctgcacagg agtgttttgg tttcaggtga acataagcgc catccgacgt
    11521 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    11581 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    11641 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga
    11701 taaaaacctt caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    11761 caataacact atcgtcccgg tgccaaaacc tggtacgacg ataactattc cttcacaact
    11821 gttattacct gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    11881 ttattatccg ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg
    11941 gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac
    12001 gcctacggca ggcattcgtc agcgttcgct ggagcgtggc attaaattac cgccagtcgt
    12061 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    12121 cgaatacctc attcatggca ccagtgcgcc ggacagcgtt ggtttgcgcg tcagttcagg
    12181 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    12241 gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta
    12301 tgttgaagta catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata
    12361 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    12421 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    12481 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    12541 tatgttacgc gcgacgcagt agtggtaaat agcagacaaa aaaatggcgc acaatgtgcg
    12601 ccatttttca cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg
    12661 ctggttagca cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac
    12721 gtcgttgctc agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc
    12781 gattttagcg ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag
    12841 taccagttta gtagctttca ttattaatac cctctagatt gagttaatct ccatgtagcg
    12901 ttacaagtat tacacaaagt tttttatgtt gagaatattt ttttgatggg aaggcactta
    12961 tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt ttagataaaa aaactgagag
    13021 aaaacaggat gcttccatcg gattcatctt agataaagtg agattatata gtgaaatccg
    13081 atttgatttt ttattgcttc tggttatcga ttaaataaaa aagcgcccat cagggcgctt
    13141 cgatatacaa attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt
    13201 agtgccgctc ggtaccagtg caccagaaac cataactacg acgtcacctt tgtgtgccag
    13261 accgctctgc agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc
    13321 tttaaccagc tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt
    13381 ggtggtcagt gccagtatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt
    13441 accgccctgg gtagcaacca cgatcagcgg agcatccagt ttttcagcag tttcaacggc
    13501 accacggcat accgcttcgg taatgcgcag tttacggttg tcattgttga actcgagacg
    13561 gctgttcatc acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag
    13621 cgggtattta ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat
    13681 ggcgtttgca acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga tcatggaatc
    13741 cagcatctgg gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat
    13801 cttctgggcg aagataactt cttctaccgg aatttctaca cccaggtcgc cacgcgcaac
    13861 catgatgccg tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc
    13921 gattttggag atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc
    13981 gataacgtca gaacgcttac gaataaagga agcagcaaca aagtctacgc cttgttcgca
    14041 accaaagatc aggtcctgtt tgtctttttc agccagtgct ggcagggcaa tggaaacgcc
    14101 aggcaggttc acacctttgt tttcgcccag gtcgccgttg ttcagcactt tacagataac
    14161 tttgttacct tcaatggcag taacttccat accgatcaga ccatcgtcaa ccagtacggt
    14221 gttgccaaca gacaggtcag tagtgaaacc ttcatacgtt accgcaacca tttcgctgtt
    14281 gccgataaca gatttatcag tggtgaaagt aaaggtctga cccgctttca gagaaacgtc
    14341 gttaccgcct tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc
    14401 ggcggtttta ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc
    14461 tgcatagtca ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt
    14521 agctaacatc tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt
    14581 catgacagtc ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact
    14641 gccggaaagc gaatccggta ttacgaaagt ggtggtgcct tgctctaagg ataggtgaca
    14701 tcgaaaaagc gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgcctgtttt
    14761 tgagtcgttg agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca
    14821 ttatacgctg aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa atatacaact
    14881 tgttagacaa taaattgtta tcaaagattt aacgttccag ggaaaaggtt acgcaaaaac
    14941 ttcgctgttt gcgcaacgtc tggtgccgaa gttgtcagtg tgaggataag tgaaagttca
    15001 tccggtgtat aaatatatca tcgctcaatt gctacagtta aaataattta tcgcgttatt
    15061 tgtctgattt tgtttgattt ttgtttaatt ggttagattg atatatgctg atagttggca
    15121 tgataaatag gtataaagaa tgggaaatcg gacaaaagag gatgaactgt accgagaaat
    15181 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
    15241 tattgttatc gcaggcgtat tacgtactgc attagccaat aaacgcattc agcgcagtga
    15301 gctggaaaag caggcgatgg aaaccgtcat taacgcactg gtaaaatgac cattcaggat
    15361 gaggcggtaa gcttcatcct gatattcttg cttcagttgc ggtccagtta atcattctaa
    15421 ataacaactg ctgaaattat acgaattgcg gtatgtattt taatctataa cgataatatt
    15481 atttttattt tctgcaatga acgtcacaaa taattgtcgc ttaagttaaa tttatttcaa
    15541 ttgataataa atatcagttc acttataatt tcttgctttg taattttaat tgataacgat
    15601 caatgttaag ggctcagaat aatcacatta ttttttctcc taaccataaa ggattggttt
    15661 atcggcgtta aatcttttct catacactat cgggttaggg aaaatatctg gtgttgggga
    15721 cagagtcagc agcgtattag ttgaatgctt aatatcaggc tgctgaattg tcagcactgt
    15781 gtttgcgaag aggatgcgaa ataaatgaac ccggttgatc gtccactatt agatattggc
    15841 ttaacgcgat tagaattctt gcgtatatca ggaaaaggcc ttgctggtct aacaattgcc
    15901 cctgcgttac tgtcactttt gggttgcaaa caggaagata ttgatagcgg cacagtaggg
    15961 ttgataaata cacccaaagg ggtgctggtg acccagcgag cgcgatgcac gggttgccac
    16021 cgctgtgaaa tctcttgtac caacttcaac gatggctcag tagggacatt cttctcccgt
    16081 atcaaaatcc atcgcaatta tttctttggc gacaacgggg ttggctctgg cggcggcctg
    16141 tatggcgatc tcaactatac cgcggacacc tgccgtcaat gcaaagagcc gcaatgcatg
    16201 aacgtctgcc cgattggtgc gattacctgg cagcagaaag aaggctgtat taccgtcgat
    16261 cataaacgct gtattggctg tagcgcctgt actacggcat gcccgtggat gatggccacc
    16321 gtaaataccg aaagtaaaaa atcctcgaaa tgtgtgttat gcggtgaatg cgcaaacgcc
    16381 tgcccaacag gggcgttaaa aattatcgag tggaaagata ttactgtgtg aatcttgcaa
    16441 aggaaaaaat catggctaac ggttggacag gtaatatatt aagagtcaat ctcacgacag
    16501 gaaatattac cctcgaagat tccagtaagt ttaaaagttt tgtcggtggc atgggcttcg
    16561 gctacaaaat tatgtatgac gaagtaccgc caggcacgaa acctttcgat gaagcgaata
    16621 aattagtctt tgctaccggc ccattaactg gatctggtgc cccctgtagt tctcgcgtaa
    16681 atatcacctc actttctact tttaccaaag gaaatttagt cgtcgatgcc catatgggtg
    16741 gcttttttgc agcgcaaatg aaattcgctg gatacgacgt cattattatc gaagggaagg
    16801 cgaaatcacc ggtatggctg aagattaaag atgacaaagt tagcctggaa aaagccgatt
    16861 tcttatgggg aaaagggacg cgcgcaacga cggaagaaat ttgtcgattg accagtccgg
    16921 aaacctgtgt ggcggctatt ggtcaggctg gggaaaacct tgttcctctc tctggcatgt
    16981 tgaatagccg taaccacagc ggcggtgcgg gaactggcgc aataatgggt tcgaaaaacc
    17041 tgaaagcgat tgcggttgaa gggacgaaag gggtcaacat tgccgatcgt caggagatga
    17101 agcgtctcaa cgattacatg atgactgaac ttattggtgc gaataacaac catgtcgtgc
    17161 caagtacgcc gcaatcgtgg gcagagtatt cagatcccaa gtcacgttgg acagcacgta
    17221 aagggctgtt ttggggcgcg gctgaaggtg gtccgattga aacgggggaa attccgccag
    17281 gcaatcagaa cacggtcggc tttcgtacct ataaatccgt ttttgactta ggtccggcgg
    17341 cagagaaata cacagtaaaa atgagtggct gccactcttg cccgatccgt tgtatgaccc
    17401 aaatgaatat tcctcgggtg aaagagtttg gcgtgcccag cacaggtggt aacacttgtg
    17461 tagccaactt tgtccatacc accatcttcc cgaacggtcc gaaagatttt gaagataaag
    17521 acgatggtcg tgtgattggt aacctggtgg gtctgaatct gttcgatgac tacggcctat
    17581 ggtgtaacta cgggcagttg catcgcgact ttacctattg ttacagcaaa ggtgtgttca
    17641 agcgtgttct gcctgctgaa gagtatgcgg aaattcgctg ggatcaactg gaagcgggtg
    17701 acgttaactt cattaaaatt aaagattttt actaccgtct ggcgcatcgt gtgggtgagc
    17761 tgagtcacct ggctgatggt tcatatgcca tcgcagaacg ctggaatttg ggtgaagagt
    17821 actggggcta cgcgaaaaat aaactctggt cgccgtttgg ctatccggtt caccatgcca
    17881 atgaagcgtc agcgcaggtc ggttccattg ttaactgtat gttcaaccgt gactgcatga
    17941 cgcataccca tatcaacttt at
//
LOCUS       DAETQN010000086        17959 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.086, whole genome shotgun sequence.
ACCESSION   DAETQN010000086 DAETQN010000000
VERSION     DAETQN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.086"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(229..1203)
                     /gene="cysB"
                     /locus_tag="K0W68_002908"
     CDS             complement(229..1203)
                     /gene="cysB"
                     /locus_tag="K0W68_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBI3074421.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1413..4010)
                     /gene="topA"
                     /locus_tag="K0W68_002909"
     CDS             complement(1413..4010)
                     /gene="topA"
                     /locus_tag="K0W68_002909"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBI3074422.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4390..4641
                     /locus_tag="K0W68_002910"
     CDS             4390..4641
                     /locus_tag="K0W68_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBI3074423.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4677..5726)
                     /gene="sohB"
                     /locus_tag="K0W68_002911"
     CDS             complement(4677..5726)
                     /gene="sohB"
                     /locus_tag="K0W68_002911"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBI3074424.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5946..6704
                     /locus_tag="K0W68_002912"
     CDS             5946..6704
                     /locus_tag="K0W68_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBI3074425.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6701..7291
                     /gene="cobO"
                     /locus_tag="K0W68_002913"
     CDS             6701..7291
                     /gene="cobO"
                     /locus_tag="K0W68_002913"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBI3074426.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7331..8203)
                     /gene="rluB"
                     /locus_tag="K0W68_002914"
     CDS             complement(7331..8203)
                     /gene="rluB"
                     /locus_tag="K0W68_002914"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBI3074427.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8416..9530)
                     /locus_tag="K0W68_002915"
                     /pseudo
     CDS             complement(8416..9530)
                     /locus_tag="K0W68_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9558..10178)
                     /locus_tag="K0W68_002916"
     CDS             complement(9558..10178)
                     /locus_tag="K0W68_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBI3074428.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10175..11056)
                     /gene="yciV"
                     /locus_tag="K0W68_002917"
     CDS             complement(10175..11056)
                     /gene="yciV"
                     /locus_tag="K0W68_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBI3074429.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11194..11238
                     /gene="trpL"
                     /locus_tag="K0W68_002918"
     CDS             11194..11238
                     /gene="trpL"
                     /locus_tag="K0W68_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBI3074430.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11215..11308
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11330..12892
                     /gene="trpE"
                     /locus_tag="K0W68_002919"
     CDS             11330..12892
                     /gene="trpE"
                     /locus_tag="K0W68_002919"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBI3074431.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12892..14487
                     /gene="trpD"
                     /locus_tag="K0W68_002920"
     CDS             12892..14487
                     /gene="trpD"
                     /locus_tag="K0W68_002920"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBI3074432.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14488..15849
                     /gene="trpCF"
                     /locus_tag="K0W68_002921"
     CDS             14488..15849
                     /gene="trpCF"
                     /locus_tag="K0W68_002921"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBI3074433.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15861..17054
                     /gene="trpB"
                     /locus_tag="K0W68_002922"
     CDS             15861..17054
                     /gene="trpB"
                     /locus_tag="K0W68_002922"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBI3074434.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17054..17860
                     /gene="trpA"
                     /locus_tag="K0W68_002923"
     CDS             17054..17860
                     /gene="trpA"
                     /locus_tag="K0W68_002923"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBI3074435.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4315 a         4750 c         4488 g         4406 t
ORIGIN      
        1 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
       61 tttgaaaact ggagtttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
      121 ataaatatga gggataaaaa tattgtgcac ggtatagaaa taagtaacaa aagagaatat
      181 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
      241 cagttttata tctttaaaca tgacctcaat ttcttcatta gagcgcaatg cgacagccgc
      301 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
      361 gcgcaagaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
      421 agcatcaaca cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
      481 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
      541 gacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
      601 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
      661 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
      721 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagtga aatcagcatt
      781 gcctttagag acggcatcag caatttgtgt cggcgagccc tggtgcatat gcaaagaaac
      841 gcggggataa cgctcaataa agcctttgat aacgtttggt aacgcgtagc gtgcctgggt
      901 atgcgtggtg gcgatataca gtgagccttt atccggccag gtgtgctctc cggcaaccga
      961 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgccctgc
     1021 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
     1081 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
     1141 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
     1201 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
     1261 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
     1321 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
     1381 ctttgtttat aaaaacctga cagaattaaa ggttattttt ttccttcaac ccatttgcca
     1441 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
     1501 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
     1561 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
     1621 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaaaacaccc
     1681 gcagcgccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
     1741 ggcaccggat cttctttcgg tggtgccact tcgccgttac gtaaaatctt acgtgtgttt
     1801 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
     1861 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
     1921 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
     1981 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
     2041 cgcagcgcgt tggtttcagc gtcttcgcct tccagtacgt tcagcacttc gttttccggc
     2101 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcataacc agaacagcca
     2161 aggaataccc cggtgctcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
     2221 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
     2281 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
     2341 tcgtgatttg ccaccaggtc gaggctgttt tccatctgtg cggtgaagtc gtagttcatt
     2401 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgccaatttt ttccgcatag
     2461 aaacgacgat tttctactcg cacgtagcca cgatcctgaa tggtcgaaat gatcgacgca
     2521 taggtagacg gacgaccgat accgcgtttt tccagctctt taaccagcga tgcttcactg
     2581 aaacgggctg gcggcttggt aaagtgctgg gctggtgtaa gttcaacgag cgtcagagca
     2641 tcgcctttat taactgctgg taagatgcga tcttcatcgc ctttacgcaa cgcaggcatc
     2701 acttttgtcc agccatcaaa acgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
     2761 gcaccaatgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggctacgaac
     2821 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
     2881 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
     2941 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
     3001 ccacgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
     3061 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaatcca
     3121 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
     3181 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
     3241 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcgttctg atgagtcacc
     3301 tgtaacgcca acgcttcacc agatggcgtg gtcgtgctgg catcgacttc ccagaactct
     3361 tccggcacga acgctttaat ttcacgctca cgctcgacca ccaggcgaac cgccaccgac
     3421 tgcacacgac cggcagacag accacgagcg atctttttcc atagcagcgg cgaaaccata
     3481 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
     3541 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
     3601 actcggctat agcgcgcatc atcaccccca atcacttccc gcaggtgcca tgcaatggct
     3661 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
     3721 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
     3781 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
     3841 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
     3901 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cactacccag atacttgttg
     3961 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
     4021 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
     4081 taccgatatg gactcgttct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
     4141 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggaattttg
     4201 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
     4261 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
     4321 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
     4381 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
     4441 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
     4501 cagcgtaatg gcgtgctggt atttatcggc gactactttt tagatgaaca aggattgcca
     4561 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
     4621 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
     4681 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
     4741 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
     4801 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
     4861 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
     4921 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
     4981 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
     5041 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
     5101 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
     5161 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
     5221 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
     5281 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
     5341 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
     5401 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
     5461 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
     5521 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
     5581 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
     5641 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt cgccaaaaac
     5701 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
     5761 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
     5821 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
     5881 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
     5941 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
     6001 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
     6061 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
     6121 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
     6181 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
     6241 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
     6301 acgtcatgca ggtcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
     6361 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttggg cgtcagggac
     6421 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
     6481 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
     6541 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
     6601 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
     6661 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
     6721 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
     6781 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
     6841 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
     6901 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
     6961 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
     7021 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
     7081 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
     7141 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
     7201 tcttgaactg gcggatacgg taagtgagct gcgccccatc aaacatgcgt ttgatgccgg
     7261 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
     7321 catactttga ttaaccgtta ttacgcccgc cagaacgacg accgccgctc acctgactgt
     7381 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
     7441 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
     7501 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
     7561 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
     7621 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
     7681 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
     7741 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
     7801 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
     7861 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
     7921 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
     7981 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
     8041 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
     8101 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
     8161 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
     8221 tcgccttccc aggcgtctga tatgcttttg tgcaacagtg agtcagaact tacatctgac
     8281 tcactgatag taaagtctgt ttgaactctt caggccttaa gtaaacgact cgacgtttta
     8341 actggcctgg ctttgaggcg caacatacta cactatcttt atggttaaag cttgtttcga
     8401 ttaacatcag cttaattacc agccaccacc gcctccgcca cccgcgccgc caccagcacc
     8461 accaccggaa taacttcgcc ccaacaaact ggttttggcc cccagactca ggtgcatctg
     8521 taatgaaaac aaaacgccac tacgggcaat ttccggggcg ctaacagccg ctgtgagtga
     8581 tggggtgata cgtgctaccc attctcttcc aaggtttgct gagactgccc aacctagcaa
     8641 cgcgtaatcc agtttccgtg tttccccttt ccgccgacgc ccactgtgtg ttgctgcctc
     8701 tttacgcgca agatagttaa cgatagcttc accgtgggca taacgttgct gcccagtttg
     8761 tgtaaacttt ggcagaaaaa tataccccat gccagtgaga taaccggtca ggaaaacagc
     8821 gctaaaataa cccgctggca tataccagta aaagaataca tctccgacat tgatgtagag
     8881 agaatagaag gccacgcctg aacaaatcag tggtaaacaa ttcttccgtc ctcaaggatg
     8941 cggccatttc gatatgtgtc gccctcttct ccagtaaaga gatcgatttc gctaaatgga
     9001 ttacccgcga tatcagggaa cttgagtgaa aatcgggtct tatagatttc aaacggccag
     9061 tggttaccag tcacgttcca gattaacaga tctgaatctc cctcacgcag gaaagcattt
     9121 ttaacctgat aatgaatttg ataatgatag tcgccttgag gcaagaaacg ttgtttatcg
     9181 cccacgacaa tactcattaa cccgatatgc cagtcatgat gccagggttc aggaatacca
     9241 tcacgggtga cgccaacaat atgatagttc agcagtgcag cgtccccacc aggctgcatc
     9301 cagagtcgtg gtaaatcacg ataaaatccg tgtttaatct gctgatttcg cgcctgaaaa
     9361 tgaatatttt ctttgatgtc gagcacccca tccggttgca gagtggcttc aacatcaaag
     9421 agcgaaatcc cttcttcata agaagggatc tccgtgtttt ctgcataact tagagatgat
     9481 acaaatagcc ctgctatcaa cagcaaaatg cagcgaaact tccctgccat agtcttccat
     9541 ccgtggaacg caatttgtta taagaaaggc ttcacatcac ctacgccttc acgcaccacg
     9601 accggcgtat catcggtaag atcaataacc gttgtcggtt tctgcccgag atagccgccg
     9661 tgaataatca aatctacctg tttttccaga cggtctttaa tttcttcggg atcggattcg
     9721 gtaaattcgc tgcctggcag cattagcgaa gtggaaagca tcggttcgcc cagtgcttca
     9781 agcaacgcct gcgcaatcgg gttagaaggc acacgcatac cgatggtttt gcgtttttcc
     9841 tgcaacagac ggcgtggcac ttccttcgtc cccttcagga tgaaggtata gttgcccggc
     9901 gtgttgtttt tcattaaacg aaacgcaacg ttatcaacaa atgaataggt cgagagttca
     9961 gaaagatcgc gacacatcag ggtaaagttg tgaccgtccg gcagctggcg aatacgacaa
    10021 atacgctcca tcgcgttttt atcttcaatt ttacagccga gcgcatagcc ggaatcagtt
    10081 ggataaacaa tcaccccgcc tttacgcacg atctccaccg cctggttgat cagacgttgc
    10141 tgtgggttat caggatgaat ataaaaaaac tggctcataa ttccctctct gtggtgttct
    10201 gcggctgttc ccatagctgc cagacgcctt caacgcctgc gggcagccag agtttacgac
    10261 ctagttcaat ccacgggcat ggctgatgaa aatcagatcc ttgtgatgcc cataaatgat
    10321 gctgacgcgc aagggccgcc agctgggtac gttcattggg cgattgctgg cactgcgcga
    10381 cttccatcgc gtcaccgtgg tgttcggcaa aatgcgcaac caatcttttc agccatttag
    10441 cactaagatt gtaccgccca ggatgagcta gcaccgcctt accgccagaa tgatgaatga
    10501 catcaatagc ttgttctatt gtacaccact gtggcggaac gtatccggtt ttcccgcgcg
    10561 ccagatattt tttaaagaca tccgccattg aactggcttt gccgcacccc acaaggaaac
    10621 gcgcaaaatg accgcgcgtc accgcaccgc cctgcgccag tcgtcgcgcg ccttccagtg
    10681 cccctggtat ttgcgctttt tcaagtcttt cggcaatcag ttgcgcccgc tgattgcgcc
    10741 gttctgtctg ctgcgcgagg aactcacaca ttagcggatg agtaatatca atattcagcc
    10801 cgacaatatg aatttcatga ttttcccaga ccgtggaaat ttccacgccg ggaataagat
    10861 tcagcgccag tcccgaacgt gaaatttcct ctcttgctgg cacgattgca gctgtgttgt
    10921 catggtcggt gatcgccagg gtaccgacgc gcatctcgac tgcacggtgc accaatactt
    10981 ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcgt
    11041 aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata
    11101 acagttctgg caaatattct gaaatcagct gttgacaatt aatcatcgaa ctagttaact
    11161 agtgcgcaag ttcacgtaaa aagggtatcg acaatgaaag caattttcgt actgaaagct
    11221 tggtggcgca cttcctgaaa cgggcagtgt attcaccatg cgtaaagcaa tcagataccc
    11281 agcccgccta atgagcgggc ttttttttga acaaaattag agaataacaa tgcaaacaca
    11341 aaaaccgact ctcgaactgc taacctgcga aggcgcttat cgcgacaacc cgactgcgct
    11401 ttttcaccag ttgtgtggga atcgtccggc aacgctgctg ctggaatccg cagatatcga
    11461 cagcaaagat gatttaaaaa gcctgctact ggtagacagt gcgctgcgca ttacagcatt
    11521 aggtgacact gtcacaattc aggcactttc cggcaacggc gaagccctgc tggcactact
    11581 ggataacgcc ctgcctgcgg gtgtggaaaa tgaacaatca ccaaactgcc gtgtgctgcg
    11641 cttcccccct gtcagtccac tgctggatga agacgcccgc ttatgctccc tttcggtttt
    11701 tgacgctttc cgtttattgc agaatctgtt gaatgtaccg aaggaagaac gagaagcaat
    11761 gttcttcggc ggcctgttct cttatgacct tgtggcggga tttgaagatt taccgcaact
    11821 gtcagcggaa aataactgcc ctgatttctg tttttatctc gctgaaacgc tgatggtgat
    11881 tgaccatcag aaaaaaaaca cccgcattca ggccagcctg tttgctccga atgaagaaga
    11941 aaaacaacgt ctcactgctc gcctgaacga tcttcgccag cagctgaccg aagccgcgcc
    12001 gccgctgccg gtggtttccg tgccgcatat gcgttgtgaa tgtaatcaga gcgatgaaga
    12061 gttcggtggc gtagtgcgtt tgttgcaaaa agcgattcgc gctggagaaa ttttccaggt
    12121 ggtgccatct cgccgtttct ctctgccctg cccgtcaccg ctggcggcct attacgtgct
    12181 gaaaaagagt aatcccagcc cgtacatgtt ttttatgcag gataatgatt tcaccctgtt
    12241 tggcgcgtcg ccggaaagtt cgctcaagta tgacgccacc agccgccaga ttgagatcta
    12301 cccgattgcc ggaacacgtc cacgcggtcg tcgtgccgat ggctcgctgg acagagacct
    12361 cgacagccgc atcgaactgg aaatgcgtac cgatcataaa gagctttctg aacatctgat
    12421 gctggtggat ctcgcccgta atgatctggc acgcatttgc acccccggca gcagctacgt
    12481 cgccgatctt accaaagttg accgttactc ttacgtgatg cacctggtct cccgcgtggt
    12541 cggtgagctg cgccacgatc tcgacgccct gcacgcttac cgcgcctgta tgaatatggg
    12601 gacgttaagc ggtgcgccga aagtacgcgc catgcagtta attgccgagg cggaaggtcg
    12661 tcgccgcggc agctacggcg gcgcggtagg ttatttcacc gcgcatggcg atctcgacac
    12721 ctgcattgtg atccgctcgg cgctggtgga aaacggtatc gccaccgtgc aagccggtgc
    12781 tggcgtagtc cttgattcta ttccgcagtc ggaagccgac gaaacccgta ataaagcccg
    12841 cgctgtactg cgcgctattg ccaccgcgca tcatgcacag gagactttct gatggctgac
    12901 attctgctgc tcgataatat cgactctttt acgtacaacc tggcagatca gttgcgcagc
    12961 aatgggcata atgtggtgat ttaccgcaac catattccgg cgcagacctt aattgaacgc
    13021 ctggcgacga tgagcaatcc gatactgatg ctttctcctg gccccggtgt gccgagcgaa
    13081 gccggttgta tgccggaact cctcacccgc ttgcgtggca agctgcccat tattggcatt
    13141 tgcctcggac atcaggcaat tgtcgaagct tacgggggct atgtcggtca ggcgggcgaa
    13201 attcttcacg gtaaagcgtc gagcattgaa catgacggtc aggcgatgtt tgccggatta
    13261 acaaacccgc tgccggtggc gcgttatcac tcgctggttg gcagtaacat tccggccggt
    13321 ttaaccatca acgcccattt taatggcatg gtgatggcag tacgtcacga tgcggatcgc
    13381 gtttgtggat tccagttcca tccggaatcc attctcacca cccagggcgc tcgcctgctg
    13441 gaacaaacgc tggcctgggc gcagcagaaa ctagagccag ccaacacgct gcaaccgatt
    13501 ctggaaaaac tgtatcaggc gcagacgctt agccaacaag aaagccacca gctgttttca
    13561 gcggtggtgc gtggcgagct gaagccggaa caactggcgg cggcgctggt gagcatgaaa
    13621 attcgcggtg agcacccgaa cgagatcgcc ggggcagcaa ccgcgctact ggaaaacgca
    13681 gcgccgttcc cgcgcccaga ttatctgttt gctgatatcg tcggtactgg cggtgacggc
    13741 agcaacagta tcaatatttc taccgccagt gcgtttgtcg ccgctgcctg tgggctgaaa
    13801 gtggcgaaac acggcaaccg tagcgtctcc agtaaatctg gttcgtccga tctgctggcg
    13861 gcgttcggta ttaatcttga tatgaacgcc gataaatcgc gccaggcgct ggatgagtta
    13921 ggtgtttgtt tcctctttgc gccgaagtat cacaccggat tccgccacgc gatgccggtt
    13981 cgccagcaac tgaaaacccg caccctgttc aatgtgctgg ggccattgat taacccggcg
    14041 catccgccgc tggcgttaat tggtgtttat agtccggaac tggtgctgcc gattgccgaa
    14101 accttgcgcg tgctggggta tcaacgcgcg gcggtggtgc acagcggcgg gatggatgaa
    14161 gtttcattac acgcgccgac aatcgttgcc gagctgcatg acggcgaaat taagagctat
    14221 caattgaccg ctgaagattt tggcctgact ccctaccacc aggagcaact ggcaggcgga
    14281 acaccggaag aaaaccgtga cattttaaca cgcttgttac aaggtaaagg cgacgccgcc
    14341 catgaagcag ccgtcgctgc gaacgtcgcc atgttaatgc gcctgcatgg ccatgaagat
    14401 ctgcaagcca atgcgcaaac cgttcttgag gtactgcgca gtggttccgc ttacgacaga
    14461 gttaccgcgc tggcggcacg agggtaaatg atgcaaaccg ttttagcgaa aatcgtcgca
    14521 gacaaggcga tttgggtaga aacccgcaaa cagcagcaac cgctggccag ttttcagaat
    14581 gaggttcagc cgagcacgcg acatttttat gatgcgctac agggtgcacg cacggcgttt
    14641 attctggagt gcaagaaagc gtcgccgtca aaaggcgtga tccgtgatga tttcgatccg
    14701 gcacgcattg cctccattta taaacattac gcttcggcaa tttcggtgct gactgatgag
    14761 aaatattttc aggggagctt tgatttcctc cccatcgtca gccaaatcgc cccgcagccg
    14821 attttatgta aagacttcat tatcgaccct taccagatct atctggcgcg ctattaccag
    14881 gccgatgcct gcttattaat gctttcagta ctggatgacg accaatatcg ccagcttgca
    14941 gccgtcgctc acagtctgga gatgggggtg ctgaccgaag tcagtaatga agaggaactg
    15001 gagcgcgcca ttgcattggg ggcaaaggtc gttggcatca acaaccgcga tctgcgtgat
    15061 ttgtcgattg atctcaaccg tacccgcgag cttgcgccga aactggggca caacgtgacg
    15121 gtaatcagcg aatccggcat caatacttac gctcaggtgc gcgagttaag ccacttcgct
    15181 aacggttttc tgattggttc ggcgttgatg gcccatgacg atttgcacgc cgccgtgcgc
    15241 cgggtgttgc tgggtgagaa taaagtatgt ggtctgacgc gtgggcaaga tgctaaagca
    15301 gcttatgacg cgggcgcgat ttacggtggg ttgattttgg ttgcgacatc accgcgttgc
    15361 gtcaacgttg aacaggcgca ggaagtgatg gctgcggcac cgttgcagta tgttggcgtg
    15421 ttccgcaatc acgatattgc cgatgtggtg gacaaagcta aggtgttatc gctggcggca
    15481 gtgcaactgc atggtaatga agatcagctg tatatcgata cgctgcgtga agctctgcca
    15541 gcacacgttg ccatctggaa agcattaagc gtcggtgaaa ccctgcccgc ccgcgagctt
    15601 cagcacgttg ataaatatgt tttagacaac ggccagggtg gaagcgggca acgtttcgac
    15661 tggtcactat taaatggtca atcgcttggc aacgttctgc tggcgggggg cttaggcgca
    15721 gataactgcg tggaagcggc acaaaccggc tgcgccggac ttgattttaa ttctgctgta
    15781 gagtcgcaac cgggcatcaa agacgcacgt cttttggcct cggttttcca gacgctgcgc
    15841 gcatattaag gaaaggaaca atgacaacat tacttaaccc ctattttggt gagtttggcg
    15901 gcatgtacgt gccacaaatc ctgatgcctg ctctgcgcca gctggaagaa gcttttgtca
    15961 gcgcgcaaaa agatcctgaa tttcaggccc agttcaacga cctactgaaa aactatgccg
    16021 ggcgtccaac cgcactgacc aaatgccaga acattacggc tggcacgaac accacgctgt
    16081 atctgaagcg cgaagatttg ctgcacggcg gcgcgcataa aactaaccag gtgctcggtc
    16141 aggctttact ggcgaagcgg atgggtaaaa ccgaaatcat cgccgaaacc ggtgccggtc
    16201 agcatggcgt ggcgtcggcc cttgccagcg ccctgctcgg cctgaaatgc cgtatttata
    16261 tgggtgccaa agacgttgaa cgccagtcgc ctaacgtttt tcgtatgcgc ttaatgggtg
    16321 cggaagtgat cccggtgcat agcggttccg cgacgctgaa agatgcctgt aacgaggcgc
    16381 tgcgcgactg gtccggtagt tacgaaaccg cgcactatat gctgggcacc gcagctggcc
    16441 cgcatcctta tccgaccatt gtgcgtgagt ttcagcggat gattggcgaa gaaaccaaag
    16501 cgcagattct ggaaagagaa ggtcgcctgc cggatgccgt tatcgcctgt gttggcggcg
    16561 gttcgaatgc catcggcatg tttgctgatt tcatcaatga aaccaacgtc ggcctgattg
    16621 gtgtggagcc aggtggtcac ggtatcgaaa ctggcgagca cggcgcaccg ctaaaacatg
    16681 gtcgcgtggg tatctatttc ggtatgaaag cgccgatgat gcaaaccgaa gacgggcaga
    16741 ttgaagaatc ttactccatc tccgccggat tggatttccc gtctgtcggc ccacaacacg
    16801 cgtatcttaa cagcactgga cgcgctgatt acgtgtctat taccgatgat gaagcccttg
    16861 aagccttcaa gacgctgtgc ctacacgaag ggatcatccc ggcgctggaa tcctcccacg
    16921 ccctggccca tgcgctgaaa atgatgcgcg aaaacccgga aaaagagcag ctactggtgg
    16981 ttaacctttc cggtcgcggc gataaagaca tcttcaccgt tcacgatatt ttgaaagcac
    17041 gaggggaaat ctgatggaac gctacgaatc tctgtttgcc cagttgaagg agcgcaaaga
    17101 aggcgcattc gttcctttcg tcacgctcgg ggatccgggc attgagcagt cattgaaaat
    17161 tatcgatacg ctaattgaag ccggtgctga cgcgctggag ttaggcatcc ccttctccga
    17221 cccactggca gatggcccga cgattcaaaa cgccactctg cgcgcctttg cggcaggtgt
    17281 gactccggca caatgttttg aaatgctggc actgattcgc cagaaacacc cgaccattcc
    17341 cattggcctg ttgatgtatg ccaatctggt gtttaacaaa ggcattgatg agttttatgc
    17401 ccagtgcgag aaagtcggcg tcgattcggt gctggttgcc gatgtgccaa ttgaagagtc
    17461 cgcgcccttc cgccaggccg cgttgcgcca taatgtcgca cctatcttca tctgcccgcc
    17521 aaatgccgat gacgacctgc tgcgccagat agcctcttac ggtcgtggtt acacctattt
    17581 gctgtcacga gcgggcgtga ccggcgcaga aaaccgcgcc gcgttacccc tcaatcatct
    17641 ggttgcgaag ctgaaagagt acaacgctgc acctccattg caggggtttg gtatttccac
    17701 cccggatcag gtaaaagcag cgattgatgc aggagctgcg ggcgcgattt ctggttcagc
    17761 cattgttaaa atcatcgagc aacatattaa tgagccagag aaaatgctgg cggcactgaa
    17821 agtttttgta caaccgatga aagtggcgac gcgcagttaa tccccctgcc gccagttccg
    17881 ctggcggcat tttaactttc tttaacgaag ccggaaaaat cctaaattca tttaatattt
    17941 atctttttac cgtttcgct
//
LOCUS       DAETQN010000087        17852 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.087, whole genome shotgun sequence.
ACCESSION   DAETQN010000087 DAETQN010000000
VERSION     DAETQN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.087"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..304)
                     /locus_tag="K0W68_002924"
     CDS             complement(<1..304)
                     /locus_tag="K0W68_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBI3074436.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(514..1032)
                     /locus_tag="K0W68_002925"
     CDS             complement(514..1032)
                     /locus_tag="K0W68_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBI3074437.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1729..2229
                     /gene="tssB"
                     /locus_tag="K0W68_002926"
     CDS             1729..2229
                     /gene="tssB"
                     /locus_tag="K0W68_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HBI3074438.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            2264..2488
                     /locus_tag="K0W68_002927"
     CDS             2264..2488
                     /locus_tag="K0W68_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074439.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            2539..4014
                     /gene="tssC"
                     /locus_tag="K0W68_002928"
                     /pseudo
     CDS             2539..4014
                     /gene="tssC"
                     /locus_tag="K0W68_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            4021..4434
                     /gene="tssE"
                     /locus_tag="K0W68_002929"
     CDS             4021..4434
                     /gene="tssE"
                     /locus_tag="K0W68_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBI3074440.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            4438..6288
                     /gene="tssF"
                     /locus_tag="K0W68_002930"
     CDS             4438..6288
                     /gene="tssF"
                     /locus_tag="K0W68_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBI3074441.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            6252..7334
                     /gene="tssG"
                     /locus_tag="K0W68_002931"
     CDS             6252..7334
                     /gene="tssG"
                     /locus_tag="K0W68_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HBI3074442.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            7359..8639
                     /gene="tagH"
                     /locus_tag="K0W68_002932"
     CDS             7359..8639
                     /gene="tagH"
                     /locus_tag="K0W68_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HBI3074443.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            8636..9160
                     /gene="tssJ"
                     /locus_tag="K0W68_002933"
     CDS             8636..9160
                     /gene="tssJ"
                     /locus_tag="K0W68_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HBI3074444.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            9163..10492
                     /gene="tssK"
                     /locus_tag="K0W68_002934"
                     /pseudo
     CDS             9163..10492
                     /gene="tssK"
                     /locus_tag="K0W68_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            10497..11258
                     /gene="icmH"
                     /locus_tag="K0W68_002935"
     CDS             10497..11258
                     /gene="icmH"
                     /locus_tag="K0W68_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HBI3074445.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            11267..14050
                     /gene="tssH"
                     /locus_tag="K0W68_002936"
                     /pseudo
     CDS             11267..14050
                     /gene="tssH"
                     /locus_tag="K0W68_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            14047..14790
                     /gene="tagO"
                     /locus_tag="K0W68_002937"
     CDS             14047..14790
                     /gene="tagO"
                     /locus_tag="K0W68_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HBI3074446.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            14795..16207
                     /gene="tssA"
                     /locus_tag="K0W68_002938"
     CDS             14795..16207
                     /gene="tssA"
                     /locus_tag="K0W68_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HBI3074447.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            16226..>17852
                     /gene="tssM"
                     /locus_tag="K0W68_002939"
     CDS             16226..>17852
                     /gene="tssM"
                     /locus_tag="K0W68_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HBI3074448.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPY"
BASE COUNT         4155 a         4894 c         4877 g         3926 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 tcgtcaggta ggccattttg tccagtacct gcggaaattc aagggagaga aactgctggc
      181 tgaccagaga gagctcgagg gaaaagagcg aagagagtga ctggttcaga tgaaaggaga
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag cgtgaaacgt aatccggttg
      301 acatagcaac ctccggggtt aaggaaaaac agacagttcg ctgttaaagc ggctgtgaca
      361 gtacagtgtt acgcttgcgt tcaggcacgc taccgccgaa gtttccggca tccgccatct
      421 cccgtgaacg taacccctta ctgacaacct ttctttctac ggcgtaatac aaaaacagca
      481 tccggcctga tagccggatg ctgttggctt aacttatgct tccagtggtg cgcgccagtc
      541 gtcggcaccg gaggtgccag atttgacgtg ttcccattca attttgcggt aggccagtga
      601 aacctcgagc aactgggtga actcgcgttt cgccgggtcc tggcaatgcg gcatatgcag
      661 tttcatatcg acaatcgtcg aatcagtcag gcgggtagtg aaataatgct cctgtttgcc
      721 ctccacagag gtacgccacc agtgaagttc agtggttggc agcatttcgc cggaggccag
      781 tgcgttgtac atcagcggaa ccgctttgtt cagcgccacg gtaaaaatga aaggcttgtg
      841 agcacgctga cccgcaggct gaccggactg aggagtggtc gggacagtaa cgttatgcag
      901 gaattcctgc accagcatct cgtcttcgtg cccctgcaca tagatgttgc cgacagaatc
      961 agcggtaaat gcacctgcag taatatttcc ctgggtctga ccagtgatgg agatataaca
     1021 cggtgttggc atttgtaaac tccttgttaa cgtggttaat tactacttac aatgacctgc
     1081 cgttaatccc agacaggcag atgcagctca cttcacggaa ccgcatcatt tttcagtatt
     1141 ttttacatac aaaccgagct agaatttacg gttgtcgtaa aaactatttt taaaatcatt
     1201 aaatctctta ttcagcaata acttaataat atatatctgg ataaccatac ccaactgttc
     1261 aaagtccgta ttgcctccat aaaatggcca aagctgaatc atgaataaat attgtattta
     1321 tcctttctga attatcattt tgataatttt tgagatattc cttaaaaata cccacttaat
     1381 caatcacaaa acaaatatat aaacaagcaa tcattagatt agcttatttt taatgaaatt
     1441 attatatact tcaataagtt acaaaaaaac tctatattta aattattgcc ataatacacc
     1501 tgaagcgaat tgcacaggtt cacacaatct atttaacgct tgcagaatac ccctctttac
     1561 gatcgataaa aagtcctttt cttatttatt ttgaagcgta aaatgcgggg tgatgaagga
     1621 gtggtgattt tattttcacc gccattaggg cggcattgcc gatatttttt tgcggtacaa
     1681 atatgccgtg ttcatggcct gtacgagata acatgacgga tcacttttat gagcaaaaaa
     1741 tttgaaggca gcgtcgcgcc ccgcgagcgt attaatatat cgtatgtccc aaaaacagat
     1801 ggccagacgg ctgaagtgga acttccgctt aacatgctcg ttgtcggcga taccggaaat
     1861 acgcaggaaa catccccact ggatgaacgt caggcagttt ccgttaacaa gcacaatttc
     1921 ggtgcagtga tggctgaagc agccattggc ctcaatttta ctgtccccgc cacgctgaag
     1981 ggaagcacaa cagacgatga actgaatgtg gcgctgaata tcaaatcact ggatgatttt
     2041 tcaccagata gcgtggcccg gcaggttccg gaagtgaata aattgctgga actgcgtgaa
     2101 gcactgactg cactgaaggg gccgatgggt aaccttccgg cattccgtac ccagctccag
     2161 gcattgctgg aaaatgaaga gtcccgcgag cagttgctca aagaaatcgg tctggtgagc
     2221 aataaataac caccactatt tcattaaaca gggttatcca ggcatgacgg cacacaaaaa
     2281 tatctccggg acataccacc tctctgttgc agacattctg caggtggttt accaggtatg
     2341 tttcagcccg tccgttgaaa tcaaccagga tggtgtggct gcgctgatta caacactgga
     2401 tcgccgtatc agtgatctat tggatgagat tatccatttt tgtgagtttc agcagagcgc
     2461 gtcgcactgg cagcgcgtac ttcactgatg gcgaaaagcc ggtttcagaa acacagctaa
     2521 aacagaagga ttttcagtat gtctttacag gaagaggaac tcgtttccag tcatgccggg
     2581 cagcctgagc aggcatcgtc gctgcttgat caaatcatgg cacagacccg tattcagccc
     2641 ggttcggaag gttatgacgt tgccctccag ggtgtgacag cctttattgc cagcatcctg
     2701 caatccactg catctgccga accggtcaat aaactggccg tggacagcat gattgcagat
     2761 attgatgaac gcatcagccg ccagatggat gtcatcatcc atgcgcctgc ctttcagcag
     2821 gtcgagtcgt tctggcgctc actgaaaacc atggtggatc gcgttgattt ccgtgaaaac
     2881 atcaaggtca acgtgcttca tgtcaccaag caggaacttc tggaagactt tgagtttgcg
     2941 ccagaaatta ttcagtccgg attttataag cacgtttatt cgtccggttt cggacagttt
     3001 ggcggtaaac ccatcgctgc cgtgctgggc gcttatgagt tcaaaaatac cgcgcctgac
     3061 atgaaactgc tgcaatacgt cagtgccgtg ggcgcaatgg cgcacgcacc gttcctgtcc
     3121 tccgtttccc cggagtttat ggggctgaac tcgtggaccg aactgcctaa tatcaaagat
     3181 ctttatgcca tctttgaagg tccggcctac accaaatggc gcgccctgcg tgactcggaa
     3241 gattcgcgct atttagggct tacggcaccc cgcttcctgc ttcgccagcc ctattcacca
     3301 acagataacc ctgttaagaa ctttaactat tacgaagatg tcagccagaa ccacgaagat
     3361 tatctgtggg gtaatacggc ctggatgctg gcatgcaata tcgcagacag ttttgccaaa
     3421 taccgctgat gtccaaacat tatcggtccg caaagcggcg gcgcagtgaa agatctgccg
     3481 atgcatctgt tcgaaacgat ggggcagatt caggccaaaa tcccaaccga ggtgctggtc
     3541 accgatcgcc gcgaatttga actggctgaa gagggtttta tcacccttac catgcgtaaa
     3601 gactctgata atgcagcctt tttctctgca aactcggtac aaaaaccgaa gcactttccg
     3661 ggaaaagatg cagaaaccaa ctataagctg ggtacgcagc ttccttatct cttcatcatc
     3721 aatcggttag cgcactacat caaggtgttg cagcgtgaac aactggggtc atggaaagaa
     3781 aggagcgact tagaaagaga gcttaatacc tggatccgtc agtatgttgc cgaccaggaa
     3841 aacccgcctg ctgacgtgcg cagccgcaaa cctctgcgcg ctgccaaagt tgaggttatg
     3901 gatgtagaag gcgaacccgg ctggtaccag gttgcgctaa gcgtgagacc tcattttaaa
     3961 tttatggggg caaattttga gctttccctg gttggccggt tagacaggga gtaacacacc
     4021 atgataccgg atcgggaaag aaccacaggt tctctttttg agcgtatgga agcctcttcg
     4081 gccagaaacc ggcagggagg cagtattcat tcgctcaggc aatccattcg ccagaaccta
     4141 cgaaacattc ttaatacccg gtccggtagt tgccggggtg cgcctgagct gggcattgat
     4201 gaaccggaag gggctgaaaa cttccgcgaa tccatgagtc gggccatcga gcaatgtata
     4261 gaacgctatg aaccccggat ctcacacgct gaagtgcagg cagtggtttc atccgcctcc
     4321 agcccgctgg atatgacgtt tcatattaca gcctgggtga catttaacga aacgcatgag
     4381 gtgcttgaat ttgatatggc accgaatggc agccagcatt accgggtgga ctgacaaatg
     4441 gaatttgaag aacgctattt ccgggaagaa ctcgattacc tgcgccagct tagcaagctg
     4501 ctggcaacgg aaaaacccca tctggcccgc ttcctggccg aaaaagatgc agatccggat
     4561 attgaacgcc tgctggaagg ggtggctttt cttaccggca atctccgcca gaaaattgag
     4621 gatgaattcc cagaactgac gcacgggctt attaagatgc tatggcctaa ttacctgcgt
     4681 ccggttccgg caatgaccct tattgaatat acgccggata tggataagtc ttctgtaccg
     4741 gtgttaatcc cccgtaatga gcagtttaca accaacgccg gggaaatcag agttgatgaa
     4801 gtgctgccct ctgatgctaa aaaggaggag ccgcctccct gtaccttcac cctctgccgg
     4861 gatatctggc tgctgcccgt tcgcctggag cagattgaaa accgcagtac gacccgtaat
     4921 ggtgttatca acatcacctt ttcggtcgca ccgggaacgg acttccgcac gctggatctg
     4981 aacaaacttc gcttctggct cggcaatgac gacaactata cccgtgacca gctttattta
     5041 tggttctgcg aatacttgca gggtgccgac ctgactgtgg gtgaacagca tattcgcctg
     5101 cctgagttta tgctaaaagc tgtcggtttt gagccgcagg atgccatgct gccctggccg
     5161 aaaaacgtcc acagcggcta ccggatcctt caggagtatt tctgttaccc cgatgcgttt
     5221 ctcttttttg atctttgtgg ttgtccggct ttgcctgacg gattgcaggc ggaattcttt
     5281 accctgcaac tgcgtttttc gcgccctttg cccgtggaca tccggctgcg ccgcgattcc
     5341 ctgcgcctgt attgcgcacc tgccattaat ttatttatcc accatgcaga agccatcacg
     5401 ctggacaacc ggcgggcaga ctatccgctg gttcccagcc gccattaccc acaacattac
     5461 gatgtatttt ccgttaacag tgtggtgagc caggtccagg atatgttcag gaaaaaagat
     5521 ctggggcgtc ctgtttcgac gcaggccgcg cgccagtggc cagcctttga aagtttcagc
     5581 catcagatag aatacagccg gaagcgggaa gtggtgtact ggcatcaccg gaccaaaaca
     5641 tccctgttcc atcgcggctt tgatcatacc cttgccttta tacatgctga tggcagttat
     5701 ccgtcagacg aatctctgct cagtaatgaa gtggtttcgg tatcgctgac ctgtaccaac
     5761 cgtgagcttc cgtcacaaat tcgttccggc gatatcaccg gcacaaccgg aaaaaatgcg
     5821 gctgttgctt catttcgcaa cattacccgc ccgacgcaac cactctggcc ggtcattgat
     5881 ggcagcctgc actggcccct actctccgcc atgaacctga attatctgtc attactggat
     5941 acggacgcgc tgaagcaggt catcgccaac tttgatcgcc acgcaatcca tcatccgcag
     6001 acggcacggc tgtcacaaca aaagctggat gccattgagc gtctggagac ccgccccgtt
     6061 gatcgcctgt ttacgggtat tcccgtccgg gggctggctt ccacgctgta tctgcacccg
     6121 gagccgtttg tctgtgaagg ggaaatgtat ctgctcggta cggtgctttc gcattttctg
     6181 tcgctgtacg ccagcgttaa ctcattccac atgctgaccg ttgtgaacac agaaagccag
     6241 gagacatgga aatggacgga aagaatcggg cagcatcctc ttatctgagt ccgggaaatc
     6301 ccccggcaga taaagagcag aatgatccgc tggcacaggt tttccataat gcctgctctt
     6361 acaatttttt tgcgatggcg gagctgttgc accgcctggc aaagggtgaa aaggggacgc
     6421 cagaattatc cctgcgcgac gatccggcac aggaaaccct gcgtttcagc gccgatgcca
     6481 gccttgcctt cccgtgcagt gatatcagtg cgctgaaacg ggatacgtca ggggcattca
     6541 ggatgaccac cacctttatg gggttacagg ggagccagtc gcctctcccc ggttattacc
     6601 tcgctcacct ggcctggaaa gcggtacatg aacaaagtcc ggtaggtgat tttctggata
     6661 tgttcagcca ccgcctgacc cagtttgtct ggcatatctg gcgtaagtac cgctatcaca
     6721 tcagttttcg taacggcggt gtggatgcgt tctcgcagcg catgtactcc cttgtcgggc
     6781 tgggccaccg ccagcttcgc gataagctgg cgattaatca cagcaaaatg ctggcgtatt
     6841 ccgggatcct ggcaaatccg ggacgatccc cggaaattat ctgtggcctg gtgtcccact
     6901 gctttgattt gtcagaagtg acactgcaaa actggcaacg tcgcaaggtg gatattgaac
     6961 cggatcagca aaatagtctg gggagctatt ctcttaaaaa tggcgaaaaa ctggcgggaa
     7021 gatcggtact gggcaacttt gttctgggta cacgagtgcc cgatctcagc ggaaaattcc
     7081 agctcagtat taccagcctg acccgaaaac agttcctctc ttttctgccg tccggcgaaa
     7141 acttcctgcc actgacgatg ttcgtgtcct tcatcctgcg cgatcagctt gcctgggact
     7201 tacatctggg gctggcgccg gagcaggtgg gcgcaatgcg cctgggcgat aacaaaagtg
     7261 cgctgctggg ctggaccagc ttcctcggca caccggaaga acgaccctca gtcaccatca
     7321 gggtacggtc gtaaattatg ataatcacgg agcagaatat gccggaagag aaactacaga
     7381 cgctttcatt gcaggtcatt aacggcagtg agctggaaag cgggcgggcg gcgcgctgtc
     7441 tgttcacaca gcagggaaat gtcggccatg gccccgaatg ccactggtcg gtacaggatc
     7501 gtcagcagag cattccggcc caggctttta ccgttatcct gcacgatggc acattttgtc
     7561 tgcgcccgca gacggcacaa ctgtggctga atcaggcaaa agtcacagca acatcagacc
     7621 taatacagtt gcgccagggc gatgagatcc agatcggacg gctgatggtg agggttcatc
     7681 tgaaccgggg agatattccc cattacgatg aggaaatggc cactcccgaa accatcgtta
     7741 ccaatcgcga tatgctcacg gataccctgc tatcaacgga gggtgcgcca cactatccgg
     7801 gaatgactca ccggcaccag cttgcagaca ccgtggtaaa cggtttttct gccgatccac
     7861 tccaggctct tcagtccgaa agcctgatta ccacgggcga tccgctttca ggcattgcgg
     7921 ctgtccggcc atcggcaccg ctgtccgatc cggcaagtaa tggggggatc aatactccgt
     7981 ttatggatct gccgcccatt tatgccagcc ccggcgatcg taatgacgac gtttctgcgg
     8041 cagaaatggc acaacgccac gttgcggtca cccccttact gcgcggtctt ggcggctcgc
     8101 ttaccatgag caattccgac gatgcggatg attttctgga ggaggccgga cgaacgttac
     8161 aggccgcaat aaaaggtctg ctcgatttgc agcagcagcg taacagcctc tcagacaaac
     8221 atttgcgccc gctggaagat aacccgctgc gcctgaacat ggattacgcc accgcgctcg
     8281 acgtaatgtt tgccgaaggt aaaagcccgg tacatctggc ggctcccgcc gccgtcagtg
     8341 aaagcctgcg caatgtccgc caccacgaag aagctaaccg ggcagcgatt gtggagtcgc
     8401 ttcgtgtcct gctggatgct ttctcaccac aaaatctgct gcgccgcttt gtgcagtacc
     8461 gccgcagcca tgaactgcgc cagccgctgg atgatgccgg agcatggcaa atgtacagcc
     8521 attattacga agaactggcc tccgatcgcc agcaggggtt tgagatgctg tttaacgagg
     8581 tctacgccca ggtctatgac cgggtgcttc gtgaaaaaca gcgggagccg gaagcatgaa
     8641 caggaaggcg tttctggcct gcgtgctgat gtgcgtcata ttaaccggct gcgaaacggc
     8701 gaaaaaaatc agtgaggtga tcaaaaaccc ggatatccag gtcgggagcc tgaaggagca
     8761 accttcggaa ataaccgtca ccctgctgac cgaaccggac accaatacca atgcggaagg
     8821 tgaatcagcc gccgtggatg tgcaactggt gtacctgact gacgactcaa aattgctggc
     8881 agccgactat gaccagatag ccagcacgcc gctaccggat gtgctgggta aaaactatat
     8941 cgaccaccag gatttcaacc tgttgccgga caccatcaaa accctgccgc cggtaaagct
     9001 ggatgagaaa acgcagttta ttggtgtcgt ggcctatttc tccgatgacc aggccactga
     9061 atggaaacaa atcgagacgg tggaaggaat cggccaccat taccgcctgc tggtgcatgt
     9121 acgtcaaagc agcatcgaaa tgaaaaaaga ggatgaataa ttatggcaac cacccgcaac
     9181 aaggtgatgt ggcaggaagg gatgctgatg cgtccacacc atttccagca gcagcagcgt
     9241 tacaacgact acctggataa ccagcgtttc cgggccatga atgatttatc ctggggattt
     9301 accgaactca ccctcaacaa tgaactgctg gcgcagggta agatcatgat tgacagcgcg
     9361 tcaggcacac tgcccgacgg caccgtcttt tctatccccg accaggacgc actgcccgat
     9421 ccgctgcacc cgcaaaattt tccggacgaa agaagccgca atatctacct cgctctgccg
     9481 gtcgccagtg atgtgagaaa tgaaatcagc gacggacggc gaatcgggcg ttaccggctg
     9541 aattatgccg atgtccggga tttgcattca gaagaaggtg acgcgcgaac attgacgctg
     9601 ggtcaactga cgccgcgcat tatgagcggt gcagaagata tgagcgccta tattacgctc
     9661 ccgctttgcc gtatcagcga tcgccgccga tggctccctg acgttggatg acgattttat
     9721 cccctcctgc cagaatattc aggtcagtaa gaaactgcgt gtttatctca aagaggtaca
     9781 gggggccatt ggcggacggg caagcgatct ggcaaaccgt attggctctc cggcgcagag
     9841 cggcatcgcg gatgtggcgg aatttatgat gttgcagtta cttaaccgta accagacccg
     9901 gtttacccat cacgcccgtc gatcccagct ccacccggaa gatttctacc ttgatcttgc
     9961 cgggttgctg ggtgaactga tgacctttac cgagccgtcg cgcctgccct gcccgcttga
    10021 tgtgtatgat catcatgacc tgaccaaaat atttaaaacg ctgttaccgg aagtcaaacg
    10081 ggcgctgcat accgtactgt cgccaagagc cgtcaatctg ccgctgcatc tgcgtgacgg
    10141 tatctggcag gccgatatcc atgacacaga actgctgcaa tctgccacct ttgtgctggc
    10201 cgtggcggca aacatgccgg tcgatcagat ccagcgccag tttatccagc agtcgaaaat
    10261 ttcctcgccg gaaaaaatcc gcaatatggt cagtgtgcag atccccggta ttccattgcg
    10321 tgcccttatg gtggctcccc gccagcttcc ttaccattcc gggttcagct atttcgaact
    10381 cgacaagagt ggccaggcct ggacagaaat ggctgccgcc ggggccgttg cactgcatgt
    10441 ttccggcagt ttcccggatc tgaacatgca actgtgggcg ataagagggt aagacgatgg
    10501 atgaggggtc actgtctctg ccaccattta ccggacacga cgaaaaaagc cagcgcaact
    10561 accatctcgc gcttcgtggc aacagcctga atccgatgat cgacgcggcc acgccgctac
    10621 tcggtatggt aatgcgcctt tcaaccatga acagccagac gatgccggag catctttttg
    10681 ctcaggtggt gacagatgtt caggccgtgg agcaactgct tcaggagcag ggctacgaac
    10741 cgggcgtgat tatctcgttt cgctacatcc tctgcacgtt tattgatgaa gcggcgctcg
    10801 gtaacggctg gtcaaacaag aacgagtgga tcaagcagtc attgctggtc catttccaca
    10861 acgaagcctg gggtggcgag aaagttttca tcctgctgga gcgcctgatc cgtgaaccca
    10921 aacgttacca ggatctgctg gaatttctct ggatctgttt ttctctggga tttcgcgggc
    10981 gctacaaagt ggcagcacag gatcaggggg aatttgagca gatctaccgc cgtctgtacc
    11041 acgttctgca taaactgcgc ggcgatgccc ctttcccgct gttacaccag gacaaaaaaa
    11101 cccagggcgg gcgttatcag ctcatcagtc gcctgactgt taaacatatt ttttgtggtg
    11161 gcgttgtcgt gctggcgttg ttttacctgt tctatctgtt gcggctggac agccagacgc
    11221 aggacatcct gcaccagcta aacaaattac ttcgataagg acgtttatga tccagattga
    11281 tcttcccacg ctggtaaaac ggctgaacct gttctcccgc caggcgctgg agatggccgc
    11341 ctctgaatgt atgagtcagc aggcagcgga aattaccgtc agccatgtgc tcattcagat
    11401 gctcgccatg ccacgcagtg acctgcgggt tattacccgc cagggcgata ttggcatgga
    11461 agagttgcgc caggcgctga cggtagagaa ctacacaacc gcccgttctg cggacagcta
    11521 cccggcgttt tccccgatgc tggttgagtg gcttaaagag ggctagctgc tggcgtcggc
    11581 tgagatgcag cacagcgaac tgcgcggcgg cgtgttgctg ctggccctgc tgcattcgcc
    11641 gctgcgttat ataccgcctg ctgccgcccg gctgttgacc ggcattaacc gtgaccgtct
    11701 gcaacaggac tttgtgcagt ggacacagga gtcggcggaa tcagtcgtgc cggatgcaga
    11761 cggtaaaggc gcaggcacaa tgacggacgc ctctgacacc ctgcttgccc gctatgccaa
    11821 aaacatgacc gcagacgccc gtaacggcag gcttgacccg gtactgtgcc gcgaccacga
    11881 aatcgacctg atgatcgaca ttctctgccg ccgccgtaaa aacaacccag tggtggtggg
    11941 cgaagcgggc gtgggcaaaa gcgcactgat tgaagggctg gcgctgcgca tcgtggcagg
    12001 ccaggtgccg gacaagctga aaaacaccga tatcatgacc cttgacttgg gcgcattgca
    12061 ggccggggcg tcggtgaagg gtgaattcga aaaacgtttc aaagggctga tggcggaggt
    12121 catttcctcc ccggtgccgg tcattctgtt tatcgacgaa gcacataccc tgattggcgc
    12181 gggcaaccag cagggcgggc tggatatctc caacctgctc aaaccggcgc tggcgcgcgg
    12241 cgagctgaaa accatcgccg ccaccacctg gagcgagtac aaaaaatact tcgaaaaaga
    12301 tgccgccctg tcgcgccgct tccagttggt gaaggtcagc gaacccaacg ctgccgaagc
    12361 caccattatt ctgcgcggtc tgtcggcggt ctatgaacag tctcacggcg tgctgattga
    12421 tgatgacgcc ttgcaggccg ctgcgacatt aagcgagcgt tatctctccg ggcgtcagtt
    12481 accggacaaa gcgattgatg tgctggatac cgcctgcgcc cgtgtggcca tcaacctgtc
    12541 gtcgccgccg aagcaaatct cggcgctgac cactctgagc caccagcagg aggcggaaat
    12601 tcgccagctt gagcgcgagc ttcgcatcgg actgcgtacc gacacatcac ggatgaccga
    12661 ggtgctggtg cagtatgatg aaacgctgac ggcgctggat gaactggaag cggcctggca
    12721 ccagcagcag acgctggtcc gggagattat cgcgctgcgc cagcagttac tgggcgtggc
    12781 agaggacgat gcggcgccgt tgccggacgc agataccgtg gaggatacgc agccagagtc
    12841 agagtcagag tcagagtcag agtcagagca ggataatacc ggtgccgaac cggctgatga
    12901 ggccgacaga gaacaaccgg aagagaccgc tgaaacagtt tccccggtac agcggctggc
    12961 ccagctcact gccgaactgg acgccctgca taacgaccgg ttgctggtct ccccgcacgt
    13021 cgataaaaaa cagattgcgg cggtgattgc cgaatggacc ggcgtgccgc tcaaccgcct
    13081 gtcgcagaat gaaatgtcgg tcatcaccga cctgacgaaa tggctgggtg acaccatcaa
    13141 aggccaggac ctggcgattg ccagcctgca taagcatcta ctgaccgcac gcgccgacct
    13201 gcgtcgtccg ggacgcccgc tcggtgcgtt cctgctggct ggccccagcg gtgtgggtaa
    13261 aaccgaaacc gtcctgcaac tggcagagct gctctacggc ggtcgccagt acctgaccac
    13321 catcaatatg tccgaattcc aggagaaaca caccgtctcg cggctgattg gttcgcctcc
    13381 gggctacgtt ggctacggtg aaggcggcgt actgaccgaa gcgattcgcc agaaacccta
    13441 ctcggtagta ctgctcgatg aagtggaaaa agcgcacccg gatgtgctca acctgttcta
    13501 ccaggcgttc gataagggcg aaatggcaga cggtgaaggc cgcctgattg actgcaaaaa
    13561 tatcgtcttc ttcctgacgt ccaacctcgg ctaccaggta atagtcgagc atgccgatga
    13621 cccggaaacc atgcaggaag tacagtatcc ggtgctggcc gacttcttca aacctgccct
    13681 gctggcgcgt atggaagtgg tgccgtatct gccgctgtcg aaagagacgc tcgccaccat
    13741 tatcgccgga aaactggccc gtctggataa cgtgctgcgc agtcgctttg gtgtagaagt
    13801 ggtcattgaa ccggaagtga cggacgaaat catgagccgc gtcacccgcg cggaaaacgg
    13861 cgcgaggatg ctggaatcgg tcatcgatgg cgacatgcta ccgccgctct cgctgctgct
    13921 gttgcagaaa atggcggcta acacggcgat tgcccggatt cggttgtcgg cagtggacgg
    13981 cgcatttacg gcagacgtgg aagatgctca gaacgacgag tccgtcacaa aggatgaaac
    14041 ggttttatga gcaggttgcg cgttttccgg cagacaggat ggtttatcgc gggcctgatg
    14101 acgggcttac ccgccactgc cgcgcccgca gaagcatcgt caatggccgg tgtggcggtc
    14161 gccgtcgcca ccacgacccc gccggatgcg acagccacgt tacaggccat gcagtcctgt
    14221 cggcgggagt ccgcggcgct ggagcggctt gactgctatg accgcctgct ggcaccgctg
    14281 tccccgtcag ggtttgacgg ggcgctggtc aaagccggtt ttgtgggcga ggcctggacc
    14341 cgcgccacag agcaggaaaa acgccgcgag ggcaacacca cagaactgct ggtgacgcag
    14401 gtgccggggg agcgcccgac ggtggtgata accaccccgg ctatcggtca tgttccgcca
    14461 cgcccggtgc tgatgttcag ttgcgtggac aatatcaccc ggatgcaggt ggcgctgatg
    14521 caccctctgg atgtccatga tatcgccgtc actctgaatg ccgacaaccg ggcattgcgc
    14581 agccactggt ttgtgcgcga gaacggcacc ctgctggagt ccagccgggg gttatccggg
    14641 attgatgaaa ttaagcaact gttcggcgca aaaacgctga cggtggatac cggcgcagac
    14701 aatgccgccg gaaagctgac ctttaacatt gacgggctgg caagggcgat tgctcccctg
    14761 cgcgatgcct gccactgggc gggagaataa aaatatggca atggacttac gcgatccgaa
    14821 tgtctggata tcgcacctgc tggaaaacct gccggaagag aaactggcat cggcgctgaa
    14881 agatgacaac ccgaactggg agtatatcga cggcgaaatc gtcaagctgg gatcgctggc
    14941 ccacagtcaa ctggatatcc cggaactcca gcgccggggg ctggtcattc tggcttcgga
    15001 gagtaaggat tttcgtctgc tggcgcatct gctaagaacc ctgcaacacg ccggtgatcc
    15061 tcatctcgca ttgcgcctgc tggcgctgta tgtggagcat tactgggcgg tggctgcgcc
    15121 gcagaatatg gcgcataaaa aacgctttgc cagccaggtc attaaacgct ttgaaacagg
    15181 tattgaagtt ttttcacaga acgccgccac agcgcagcgc gatgcgctgt caggggaact
    15241 ggcaaaactg gcgcaatgct ggcagtcaca taacgccccg gaactggcac aggctactga
    15301 tgatcttttt gccctgtacc agcgtgcgtt taatcgtgcg gcaccggctc ctgtccccac
    15361 tccggcggcc tccggtagtt caccacagac caccgccacg tctgaaagcg gcgtgacgca
    15421 acccagtgct ccggctcccc aaatcgtcat cgacagtcac gacgacaaag cctggcgcga
    15481 cacgctgtta aaagtggcgg ccattttatg tgaacgccag ccggattcac cgcagggcta
    15541 ccgtctgcgc cgccatgcgc tttggcagaa catcaccagt acgccccagg cggaaagcga
    15601 tagccgtact ccactggctg cggtctccgc cgatatggtg gcagattacc atgctcagct
    15661 cggtagtgcc gatatggcgc tgtggcagca ggtggagaaa agcgtactgc tggctcctta
    15721 ctggctggat ggtcactgcc tttctgcaca gacggcgctg cgtctgggtt acaaacaggt
    15781 ggcagacgcc atccacgatg aggtcatccg cttccttgag cgtctgcccc agcttaccgg
    15841 gctgctgttt aatgaccaca caccgtttat cagtgagcag acgaaacaat ggctggcagc
    15901 ttcgcccgac gccaaagttg caccggttgc gcaaatcggt gaggaatcga aggcagccag
    15961 agcctgtttt gctgagcagg gtctggaggc ggcgctgcga tatctggaca tgctacccga
    16021 aggcgatccc cgcgatcagt ttcaccgcca gtaccttgcc gcacagttga cggaagaggc
    16081 ggggctggta cagcttgcgc agcagcagta ccggatgttg ttcaggatgg gattgcagat
    16141 gatggtggca gactgggagc cgtccctgct tgagcagcta gaacaaaaat tcacggcaga
    16201 acaataaaag agaacaggag tttttgtgtt cagattacca acaccccgac tactcagcgg
    16261 actcaaatca gccctgcgac cggcgatgcc ccggtttaaa gtctctgctt tctggctgct
    16321 gatactggcg tggatttttc tgctggtgtg gatctggtgg aaaggcccga tgtggacgct
    16381 gtatgaagaa cagtggctca aaccactggc gaaccgctgg ctggcaacgg cggcgtgggg
    16441 gattattgcc ctggtgtggc ttaccgtccg ggtgatgaag cgcctgcaac agcttgaaaa
    16501 aatgcaaaag caacagcgcg aggaagccgt tgatccgctc agcgtggaac tgaacgccca
    16561 gcagcgttat cttgaccgct ggctgctgcg cctgcaacgc catctcgaca accgccgttt
    16621 cctgtggcag ttgccgtggt atatggtcat cggcccggcg ggcagtggca aaacgacact
    16681 gctgcgcgag gggtttccgt ccgacattat ttatgccccg gagggcgcac gcggcgcaga
    16741 acaacgcctg tacctcacgc cccatgtcgg taaacaggcg gtgatctttg atatcgacgg
    16801 cacactctgc gctcccgctg atgcggatat cctgcatcgc cgtctgtggg aacatgccct
    16861 cggctggctg aaagaaaagc gcgcgcgcca gccgctgaac gggattattc tgacactcga
    16921 tttacccgat ctgctgaccg cagacaaacg ccgccgcgag catctgttac agacgctgcg
    16981 cagccgctta caggatatac gccagcatct tcactgccag ttaccggttt acgtggtact
    17041 gacccggctt gatttattac agggttttgc cgccctgttc cagtcactca acagacagga
    17101 tcgcgatgcg attctgggcg tcacgttcac ccgccgtgcc catgaaaatg atgactggcg
    17161 aacagagttg aatgctttct ggcagacatg ggtggatcga atgaatctgg cgttgccgga
    17221 tctgatggtc gctcagactc acacccgcgc gtctttattc agtttttccc gccagatgca
    17281 gggaagccgt gaaccgctgg tgtcactgct tgagggtctg cttgatggcg aaaatatgaa
    17341 cgtgatgctg cgtggtgtct atctcacctc ttcgcttcag cgtggacaga tggatgatat
    17401 attcacccag tctgccgccc gccagtaccg gctgggcaat aacccactgg cgtcctggcc
    17461 cctggtggac accgcgcctt atttcacccg cagcctgttc ccgcaggcat tactcgcaga
    17521 gcctaatctg gcaacagaga gccgcgcctg gctgatacgt tcccgtcgcc gcctgacggt
    17581 tttctctgcc acaggcggcg tggcagcact gctgctcatc accggctggc atcactatta
    17641 caacggtaac tatcagtccg gcatcaccgt gcttaagcag gccaaagcct ttatggacgt
    17701 gccgcctccg cagggggaag atgactttgg caacctgcaa ctgccgctcc tgaacccggt
    17761 acgcgatgcc acactggcct atggcgactg gggcgaccgc agccgtctgg ccgatatggg
    17821 actgtaccag ggacgacgta tcgggcctta tg
//
LOCUS       DAETQN010000088        17448 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.088, whole genome shotgun sequence.
ACCESSION   DAETQN010000088 DAETQN010000000
VERSION     DAETQN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.088"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..502)
                     /locus_tag="K0W68_002940"
     CDS             complement(<1..502)
                     /locus_tag="K0W68_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBI3074449.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="K0W68_002941"
     tRNA            complement(688..772)
                     /locus_tag="K0W68_002941"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="K0W68_002942"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="K0W68_002942"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBI3074450.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="K0W68_002943"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="K0W68_002943"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBI3074451.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /gene="idnD"
                     /locus_tag="K0W68_002944"
     CDS             2771..3802
                     /gene="idnD"
                     /locus_tag="K0W68_002944"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HBI3074452.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="K0W68_002945"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="K0W68_002945"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBI3074453.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="K0W68_002946"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="K0W68_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HBI3074454.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="K0W68_002947"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="K0W68_002947"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HBI3074455.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="K0W68_002948"
     CDS             7117..8619
                     /locus_tag="K0W68_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBI3074456.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="K0W68_002949"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="K0W68_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBI3074457.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="K0W68_002950"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="K0W68_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBI3074458.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="K0W68_002951"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="K0W68_002951"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBI3074459.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="K0W68_002952"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="K0W68_002952"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBI3074460.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="K0W68_002953"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="K0W68_002953"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBI3074461.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="K0W68_002954"
     CDS             complement(16186..17382)
                     /locus_tag="K0W68_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HBI3074462.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDATYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4483 a         4337 c         4453 g         4175 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccatttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa tcctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg cgggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac accctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagcatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DAETQN010000089        17293 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.089, whole genome shotgun sequence.
ACCESSION   DAETQN010000089 DAETQN010000000
VERSION     DAETQN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.089"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            92..1132
                     /locus_tag="K0W68_002955"
     CDS             92..1132
                     /locus_tag="K0W68_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBI3074463.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="K0W68_002956"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="K0W68_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBI3074464.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="K0W68_002957"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="K0W68_002957"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBI3074465.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="K0W68_002958"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="K0W68_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBI3074466.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="K0W68_002959"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="K0W68_002959"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBI3074467.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="K0W68_002960"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="K0W68_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBI3074468.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9086)
                     /locus_tag="K0W68_002961"
                     /pseudo
     CDS             complement(8439..9086)
                     /locus_tag="K0W68_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9217..10635)
                     /gene="araE"
                     /locus_tag="K0W68_002962"
     CDS             complement(9217..10635)
                     /gene="araE"
                     /locus_tag="K0W68_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HBI3074469.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="K0W68_002963"
     CDS             complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="K0W68_002963"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBI3074470.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11776..12612)
                     /gene="kduI"
                     /locus_tag="K0W68_002964"
     CDS             complement(11776..12612)
                     /gene="kduI"
                     /locus_tag="K0W68_002964"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBI3074471.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12899..14080)
                     /locus_tag="K0W68_002965"
     CDS             complement(12899..14080)
                     /locus_tag="K0W68_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBI3074472.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14335..15564
                     /locus_tag="K0W68_002966"
     CDS             14335..15564
                     /locus_tag="K0W68_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBI3074473.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16024..16656
                     /locus_tag="K0W68_002967"
     CDS             16024..16656
                     /locus_tag="K0W68_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3074474.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            16990..>17293
                     /locus_tag="K0W68_002968"
     CDS             16990..>17293
                     /locus_tag="K0W68_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3074475.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT         4581 a         4511 c         3975 g         4226 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctag gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acatggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggtacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt attccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgcc
     8821 actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg ctgtaagcca
     8881 agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg ctggcactct
     8941 tctatttcat gaaaatcgac gctatgtagc agcacttgcg cagagtgaag cccaccaagc
     9001 cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc ccagctcatt
     9061 cctcccagca aaccaattgt tttcattcct ctctctcctg atgtgtgtta ccgcaacaag
     9121 gctgaataag gaacgagaca aacgcctcaa cggccaggtg cccaatctct attaacgaaa
     9181 aaagggccgg atgtacagca catccggccc gtgaaatcag acgccgatat ttctcaactt
     9241 ctcgcctgcc atcagtttgc gttcgatatg ttccagcgtg acatttttgg tttccggaat
     9301 gagccagaaa gtgatgccca caaacgcaat gttcagcgca gtgtagagcc agaacgtacc
     9361 ggcagcacca atgctgtcga gcagcgtcag gaaggtcgcg ccgataatca tattcgacac
     9421 ccagttcgtg gtggtcgaac aggtaatacc gaaatcgcgg catttcagcg gctgaatttc
     9481 agagcacagg atccacacca ctggcgcggc gctcatcgca taaccggcaa tacacatcat
     9541 cgtcatgcca acagagagcc aggacaagcc actggaagcc gtaccgttat caaactgcat
     9601 caggcaatag cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc
     9661 cggtttacgc cctgctttat ctaccgtaaa caccgcaata aaggtggcga acataaaggt
     9721 cagccctacg accagagtcg caatcatctg ttgttctgtg gtcgtaaagc ccgccatttt
     9781 gaagatacgc ggcgcgtagt acatgatgat gttcataccg gtaaactgct gcatcgcctg
     9841 caacaacata ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa acagtgccca
     9901 accgccctgt tttaacttca ggctttcacg aatttcgttg agttcttctc gcgctttttc
     9961 cgacgtatcg cgcagcatac gcaacacttc ttccgcctca atatgacgcc ccttttccgc
    10021 cagccagcgc gggctatttg gcaggaagac caccagaata atcagcagga ctgctggtaa
    10081 agcaagaacc cccaacattg cgcgccagtt accgctataa ctgaacgctg tatcggataa
    10141 aaacgccagc acgatgccga gtgtgaccat caactggtac atactgatca tcttaccgcg
    10201 aacgttttca cttgccattt cagaaagata cagaggagcg gtgtaagacg cgatcccgac
    10261 agcaatgccc agcaccaccc gagcggcgat taacatctct acgctggtcg caaaagcgga
    10321 ccctatagaa ccgagtacaa acaggatggc ccccgccatc aggctgtatt tacgccccag
    10381 gcggaacgac agccaaccat taaacagcgc accaattgct gcgccgagca tcatgctact
    10441 aaccacccat tcctgcaaac gactggtcag cacaaagtga tcggtaatga acggcaacgc
    10501 tccggcgatt acgccgatat caagaccaaa taacaatcct gcgaccgcag cagctaccga
    10561 aacaaacata ttcatacgcc gcgtatcccg caaagaacgt ggcgttaaag cagattccgt
    10621 attgatagta accatttttt cctgccagca gagagtaaga catagtgaaa aaatacgtga
    10681 acaactcaca caggtgtcag gtcggaaaca gcataaatat ggattaaatt gctgcgacat
    10741 gtcgttatgt gatggatatt ccaattttca aattaagttg aattattgag attattatta
    10801 accacctaat tttacagcag ataaaattca taaagttcat taattgataa ttgatatgga
    10861 ttatttcata accatgatat gaattatgat gacctacagg tataaaaaac cctgccatga
    10921 ggcagggtca taaaagtaag aggaatgaat taacgagcca gccaaccgcc atctacggca
    10981 atggtataac cattcacata atctgaagcg ctggaggcaa ggaacactat cggccccatc
    11041 aggtcactcg gcagtcccca acgaccagct ggaatgcggt cgagaatttc cgcgctacgt
    11101 tgttcatctg cccgtagttg ttgagtattg ttggtcgcca tgtaacccgg agctatcgca
    11161 ttaacattaa tgttgtgttt agcccattcg ttcgccatca atcgcgtcac gcccatcacg
    11221 ccgctttttg atgcggtata agaaggcaca cggatcccgc cctggaagga gagcattgac
    11281 gtgatattga taatcttgcc gccattgcgt tgcgcgataa agtgtttcgc cgctgcctga
    11341 gacatgaaga atacgctctt gatattcagg ttcatgacat cgtcccagtc tttttcgctg
    11401 aactcgagag catcttcgcg gcgaatcaat ccggcgttat tcaccaggat atcaatatga
    11461 ccaaactcag ctaccgcgcg atccagcagt gctggaatgc catcaatctt tcgcagatcg
    11521 gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct gcttgatggt ttcagtcggt
    11581 tcaacgatgt taatgccaac aatgtcacag cccgcttgcg ccagccccaa cgccatcccc
    11641 tggcccagtc cagtatcaca accagtgacg accgcaactt taccttcgag agaaaatgca
    11701 cttaaaatca taacaatacc ttattcgttt atgccgacaa ctagcgcaaa tctttaacgg
    11761 ccactatgcc gacaactagc gcaaatcttt aacggccaca tggtccatat catcaaagac
    11821 ctggttttca ccaaccatgc cccagataaa ggtataagct ttggttccga cgccggaatg
    11881 gatcgaccag ctgggggaga tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc
    11941 ttgcggctgc cccatcatgt ggaaaacgca ggcgtcatca tccatattga aatagaaata
    12001 aacttccatc cggcgctcgt gagtgtgaca cggcatggtg ttccacaagt tacctggagc
    12061 cagctccgtc agccccatac tcaattggca ggtttccagc acatccggga caaaatattt
    12121 gttaattgtg cgacggttac tggtgaggtt atcgcctaac gtgactggag atacttcgtc
    12181 cggtgtgact tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt
    12241 tgccggagtg gcggtatcaa tactggcaaa gacaacttct tttgcacctt taccaacata
    12301 cagggcgtcg cggtgaccga tttcatagca ttggccatcg acagtaatcg taccggcacc
    12361 gccaatattg ataacaccta actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt
    12421 accaacttcc ccgccaacgg aaaccgtttt agttatcggc ataatgccgc caacaataat
    12481 tcggtcaatg tggctgtaaa ccatggtgta ctcatcggcg acaaatacct tttcaaccaa
    12541 aaattcattg cgcagccctt gggtatccag cgtttttgcg tgcgcactgt ggatgctctg
    12601 tcttacgtcc acatcaacct ccgataatca gtgaagcgtg aaaatagaaa acgatagtga
    12661 aacactgttt cgttttctct ataaagggat gatatgccca tgggctaggg ttttcaatta
    12721 aaaataaaac aatgtttcat ttttaagtta aggattaaaa aaagtgcagt gtcgatcacg
    12781 aatatgtcat gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc
    12841 atcattattc aacagattaa tgacggcaga tatttatcgg taaggaggaa atgcagagct
    12901 actcgtcacg ttcaatggtc aatgccacgc cctggccccc gccgatacaa agcgttgcca
    12961 gccctttgcg ggcattacgt ttcaccattt catgaaccag agaaaccagg attcggcaac
    13021 cggaagcgcc tatcgggtga ccgagtgcga tcgcgccacc attgacattg acccgacgct
    13081 catcccactc aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct
    13141 cgataagatc gacatcagcc aactgccagc ctacacgctc caggcaacgg cgggtcgcat
    13201 acaccggcgc aattcccatc aatgccggat ctacaccaac gctggcaaat gcgcggatgc
    13261 gggccagcac gggtaaattc aacgctcgtg ctttggcttc gctcatcatc attaccgcag
    13321 ctgcgccatc gtttatggat gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg
    13381 aaggatttaa acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat
    13441 caacaaccaa cgtctgaccg ttactttgga tcattaccgg gacgatctca tctttaaatc
    13501 gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca
    13561 actgacggct gatgccatat tcgcgagcca gattttcggc ggtgacacca atatgataat
    13621 cattgaaggc atcccacaac ccatcatgca ccagactgtc aaccaactgg ctattgccaa
    13681 gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg ttttcctggc
    13741 caccggcgat gacaatatca gcctcgccac actgtatcgc ctgagtagcc agatgcagtg
    13801 ctttaagtcc ggaaccgcaa acgtcattaa tcgtgattgc agaaacgcta ttaggaagac
    13861 cacctttaat agccgattgc cttgccggat tctgccctgc ccctgcagtc aacacctgac
    13921 caagaattac ttcatccacc gcatatgcag gaacgccggt acgttctatt aacgctttca
    13981 cgaccagact accaagttcc acggcggaat gacccgctaa cgcaccacga aagcagccga
    14041 taggtgtccg taacgccccg acaatcacaa cgtctttcat cacaacctcg caccaattaa
    14101 cagtgcaata gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa aaagtgattt
    14161 ttatcacaaa ggaaatatgc ctgagcagta gtcagagtca taactggcac gtaaggtttg
    14221 caaccactaa cccaccaata gatgggtaga taaggcgtta atctcccata cttaacctgg
    14281 tttatggtaa attgccctcc attttgttta atttgtagat gatacgttca gataatgtct
    14341 aatatttggt ctaaagaaga aactctgtgg agtttcgcgc tctacggcac agccgttggt
    14401 gcaggcacgc tcttccttcc tattcagtta ggttcggcag gggctgtggt cctgtttatt
    14461 actgctctgg tcgcctggcc tttaacatat tggccacata aagccttatg ccagttcatc
    14521 ctctcatcaa aaacatcagc atgtgaaggg ataacgggcg cggtaacaca ctactatggc
    14581 aagaagattg gtaatctgat taccacgctg tacttcatcg ccttttttgt cgtcgtgttg
    14641 atatatgcag tggcaattac caactcactt acggaacagc tggcaaagca tatggttatt
    14701 gatcttcgca tccgtatgtt ggtgagtctg ggtgttgtat taattctgaa tctcattttt
    14761 ctgatgggac gtcatgccac tattcgggta atgggatttt tggtattccc attgattgcc
    14821 tatttcttat ttctttccat ttaccttgtc ggtagttggc aacctgatct attaacgacc
    14881 caggtagagt tcaatcagaa tacccttcac cagatatgga tatcgattcc cgtgatggtt
    14941 ttcgccttta gccatacgcc cattatttct acgtttgcca tagacagacg tgaaaaatat
    15001 ggcgaacacg ctatggataa atgcaaaaaa attatgaaag tcgcttatct catcatctgc
    15061 ataagtgtac tgttctttgt ctttagctgc ctgctttcta ttccaccttc gtatattgaa
    15121 gcggctaaag aagaaggggt caccatttta tcggcgcttt ctatgctgcc gaacgcccca
    15181 gcatggttgt caatttccgg gattattgtc gcagtagttg cgatgtcgaa atcattcctg
    15241 ggtacgtact ttggcgttat tgaaggtgcc acggaggtcg tcaaaacaac actacagcag
    15301 gttggtgtaa agaaaagtcg tgcatttaac cgcgcactat caattatgtt ggtatcgctg
    15361 attaccttca ttgtttgttg cattaacccg aacgcgattt cgatgattta tgcgatcagc
    15421 ggcccgctca ttgccatgat acttttcatc atgcctacgt tgtcaacgta tctgatcccg
    15481 gcgcttaaac cctggcgctc catcggaaat ctgattacgc tgatcgtggg tatcctgtgc
    15541 gtatcggtaa tgttctttag ctaatatccg ttgtggatgg ttcaaaattg ctacaatttt
    15601 gaaccattcc atttttcata atattcattt gttattattc cgccctatca cttctctcgt
    15661 catatcctag aattatcagt tagttaaaca cacaaagaga tctgaacatc ttaaataaga
    15721 ctatttaaga tgcataactt agattcgcaa gatatcttct ggtcattcaa aaacaatttg
    15781 cgatatagat attctcatac catgagatag ttatctggac cacgttcgac catgcaagat
    15841 taaatcagtg aaacataatc atatttaata acgagaaatg catttttaaa tgtcttaatc
    15901 agaattatag caaatacaga ttaatcgcaa tttttcccag caaaattaca aaatcaagat
    15961 gtattgacat catcaatcac caatgaaatt atgcaattat atacggatag ggaggttctt
    16021 aacatggggc tttgtagtcg ttataaaagt cttacatgca atagttgctc aatgcattgc
    16081 caaataatgc cagaagagtc accgcgtttg cagtattgcg caaactcgtg tttttgtatg
    16141 tggcccgaag aaagttcata ttttaatcgt ggtgtggtgg aaggtatctt aacaaaaaac
    16201 cacaatgcga gattaagcgg atatattttt gtggattttt cagtaagttt tttacgtcta
    16261 ttccttgaaa aagactggat tgactatctt gcaagtactg acatggggat agttttagtc
    16321 agcgaccgca atatgcaatc gttagctaac tactggcgaa aacacaacac cgcaatatct
    16381 gctgtcatat ataatgatga tggtcttgat gttgccaatg aaaagatcag acaactattt
    16441 attggtcgtt atttatcatt taccagaggt aacacattaa ctcagatgga attcaccata
    16501 atggggtata tggtttctgg ttataatcca tatcagattg ctgaagtgct ggatatggat
    16561 atccgtagca tctacgcgta caagcaacga atcgaaaaga gaatgggtgg taaaataaac
    16621 gaattattta ttcgttcaca ttcggtccaa cattgatact caacgaccag ccagaatcat
    16681 actctgttca tacgggcatc tattgaaatg cccgtatttt ataaataagc agtaacccca
    16741 agagtaaaca acatagtatt taaaaccatc acacgaaaaa ttcagaagca ttacgaaata
    16801 tgaatttaca aacaatagtg gcataaatgt taaccatgtt aatttacgta aagttttacg
    16861 ttgcaacatt aaagcctcat ttcaatcatc atgataaata taaaattaat atatatttat
    16921 gccgtaaata ccaatatact tagcaaacta tgtgatctcc atttcgattg atttagtgtt
    16981 tattgacgta tgtactggat tattaacgat aatatcgagt tctggcctga gcaccgaaaa
    17041 ttaatatcgg tacataacgc cgatcttaac gtcgttctga caacgccagc cagtcgatgt
    17101 ttatcacttc tacttgaagc ttttcctgat gtggttgcac aacaagattt tttcaccaga
    17161 gtctgggaag aagaaggtat gcgtgtgcct actaacacgt tatatcagaa catatccatt
    17221 atcagacgcg gatttcgcgc tgttggtgat actacccact cgctaattgc aaccgtgccg
    17281 agagaggatt caa
//
LOCUS       DAETQN010000090        17168 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.090, whole genome shotgun sequence.
ACCESSION   DAETQN010000090 DAETQN010000000
VERSION     DAETQN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17168
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.090"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..234
                     /locus_tag="K0W68_002969"
     CDS             <1..234
                     /locus_tag="K0W68_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HBI3074476.1"
                     /translation="VPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQE
                     NHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
     gene            234..5504
                     /gene="traI"
                     /locus_tag="K0W68_002970"
     CDS             234..5504
                     /gene="traI"
                     /locus_tag="K0W68_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBI3074477.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRTATSEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5524..6270
                     /gene="traX"
                     /locus_tag="K0W68_002971"
     CDS             5524..6270
                     /gene="traX"
                     /locus_tag="K0W68_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HBI3074478.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            6325..6885
                     /locus_tag="K0W68_002972"
     CDS             6325..6885
                     /locus_tag="K0W68_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBI3074479.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGETPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            7017..7229
                     /locus_tag="K0W68_002973"
     CDS             7017..7229
                     /locus_tag="K0W68_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBI3074480.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
     gene            7475..7936
                     /locus_tag="K0W68_002974"
     CDS             7475..7936
                     /locus_tag="K0W68_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBI3074481.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            7982..8191
                     /gene="hha"
                     /locus_tag="K0W68_002975"
     CDS             7982..8191
                     /gene="hha"
                     /locus_tag="K0W68_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBI3074482.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            8229..8819
                     /locus_tag="K0W68_002976"
     CDS             8229..8819
                     /locus_tag="K0W68_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBI3074483.1"
                     /translation="MKLIIFILIVLIIAALLIRIILKSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            8974..9447
                     /locus_tag="K0W68_002977"
     CDS             8974..9447
                     /locus_tag="K0W68_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074484.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNEDAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMMVRKFGK
                     QLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHTKNELTGGKRRYRIKNGCK"
     gene            9694..9843
                     /locus_tag="K0W68_002978"
     CDS             9694..9843
                     /locus_tag="K0W68_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBI3074485.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            10128..10376
                     /gene="repA"
                     /locus_tag="K0W68_002979"
     CDS             10128..10376
                     /gene="repA"
                     /locus_tag="K0W68_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBI3074486.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            10621..10695
                     /gene="tap"
                     /locus_tag="K0W68_002980"
     CDS             10621..10695
                     /gene="tap"
                     /locus_tag="K0W68_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBI3074487.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            10688..11545
                     /gene="repA"
                     /locus_tag="K0W68_002981"
     CDS             10688..11545
                     /gene="repA"
                     /locus_tag="K0W68_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBI3074488.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            12246..12386
                     /locus_tag="K0W68_002982"
     CDS             12246..12386
                     /locus_tag="K0W68_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074489.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSYETTECRLP
                     CR"
     gene            12484..13137
                     /locus_tag="K0W68_002983"
     CDS             12484..13137
                     /locus_tag="K0W68_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBI3074490.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            13230..13487
                     /gene="pemI"
                     /locus_tag="K0W68_002984"
     CDS             13230..13487
                     /gene="pemI"
                     /locus_tag="K0W68_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HBI3074491.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            13420..13821
                     /gene="pemK"
                     /locus_tag="K0W68_002985"
     CDS             13420..13821
                     /gene="pemK"
                     /locus_tag="K0W68_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HBI3074492.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
     gene            14106..15416
                     /locus_tag="K0W68_002986"
     CDS             14106..15416
                     /locus_tag="K0W68_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBI3074493.1"
                     /translation="MANENLDIDSKTAEELYEWYLDDKLIINRRYQRKLVWSLEEKTA
                     LISSMTQQYPIPLLLFVSIDNKREILDGMQRLEALMSFIEQRFSLNGKYFNLDAIALT
                     KQLKDSGKLQQKEPVLSRESSTLIARYRFAISEYSSTEDHIDEVFRRINSNGKILSKQ
                     ELRSAGCVSNFSELVRKISTIIRGDTTHSDIMGLNKIHNISICNDGLDYGINIDNHFY
                     IRNHIISRPSIRDSDDEELVANILGYIFLDDKPTSGSTSLDTFYGEGSTSHAIHTRTQ
                     LENYIQTNGADKIVNNYLFVYEMIQKLFDANNLNFRSHILGNASSSQECPRYYQAVFL
                     ALYELIINENMQLDDEQKFIAQLGDSVQRSMVQTEGGRWAASARQKSVEDLCALIRRY
                     FKESENKFINHAWQTLIRTLLNNSRTEQPNYDFKQGGDAANLLI"
     gene            15445..15543
                     /locus_tag="K0W68_002987"
                     /pseudo
     CDS             15445..15543
                     /locus_tag="K0W68_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(15693..16553)
                     /locus_tag="K0W68_002988"
     CDS             complement(15693..16553)
                     /locus_tag="K0W68_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HBI3074494.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(16736..>17168)
                     /locus_tag="K0W68_002989"
     CDS             complement(16736..>17168)
                     /locus_tag="K0W68_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3074495.1"
                     /translation="TDREGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQ
                     GVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRR
                     TVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
BASE COUNT         4337 a         4036 c         4850 g         3945 t
ORIGIN      
        1 gttccggcag gggggatcga gcaggagctg aaaatgaaac cggaagaaga gatggaacag
       61 caactgccac ccgggatcag tgaatccggt gaagtggtgg atatggccgc ttatgaggca
      121 tggcaacagg aaaatcatcc ggacatccag cagcatatgc agcgtcgtga agaggtgaac
      181 attaatgtgc accgggagcg cggggaggat gttgagccgg gagatgattt ctgatgttga
      241 gtttcagcgt tgttaaatcg gcaggcagtg ccggaaacta ttacaccgac aaggataatt
      301 actatgtgct gggcagcatg ggagaacgct gggccggtca gggggctgaa cagcttgggc
      361 tgcagggcag cgtcgataag gatgttttta cccgtcttct ggagggcagg ctgccggacg
      421 gggcggattt aagccgcatg caggatggca gtaataagca tcgtcccggc tacgacctga
      481 ccttctccgc ccccaaaagt gtctccatga tggccatgct cgggggggat aaacgtctta
      541 ttgatgcaca taaccaggcc gtggattttg ctgtccgtca ggtggaggcg ctggcttcca
      601 cacgggtgat gacggacgga cagtcagaaa cggtgctgac cggtaatctg gtgatggcac
      661 tgtttaacca tgacaccagt cgcgatcagg acccacagtt acacacgcat gtggtggtgg
      721 ctaatgtcac gcagcataac ggcgagtgga agacactgag cagtgacaaa gtggggaaaa
      781 ccggattttc cgagaacgtg ctcgcgaacc ggattgcgtt cgggaaaata tatcagagtg
      841 agttacgtca gcgcgttgag gcgctgggtt atgaaaccga agtggtcggt aagcacggta
      901 tgtgggagat gccgggcgta ccggtggagg ccttttccag tcgttcacag gctatcaggg
      961 aggccgtcgg tgaagacgcc tcgctgaaat cccgggatgt ggcggcactg gatacgcgaa
     1021 aatccaaaca gcacgtcgat ccggaaatca gaatggctga atggatgcag acgctgaagg
     1081 aaaccgggtt cgacatccgg gcatatcgtg acgcggcgga tcagcgtgcg gagatccgca
     1141 cgcaggcgcc cgggcctgct tcacaggacg ggccggatgt gcagcaggcg gtgacacagg
     1201 cgattgccgg attaagtgaa cgaaaagtgc agttcacgta tacggacgtg ctggccagga
     1261 cggtcggcat actgccgccg gaaaatggtg tgattgaacg ggcacgcgcc ggtatcgatg
     1321 aggccatcag ccgtgagcag cttatccccc tcgaccgtga gaaggggctg ttcacatcag
     1381 gaattcatgt gctcgatgag ctgtccgtcc gggcactcag tcgtgacatc atgaaacaga
     1441 accgggtgac cgtacatccg gagaaaagtg tcccccggac ggctggttac agcgatgcgg
     1501 tcagtgttct ggcacaggac cgtccgtcgc tggccattgt gtccgggcag ggcggtgcag
     1561 ccgggcagcg tgagcgggtg gctgaactgg tcatgatggc ccgggaacag gggcgggagg
     1621 tgcagattat cgctgctgac cgtcgctcgc agatgaacct gaagcaggat gaacggctgt
     1681 ccggtgaact gataaccgga cgtcgtcagc tgctggaagg catggccttc acgccgggca
     1741 gtaccgttat cgtcgaccag ggtgaaaaac tctccctgaa agagacgtta accctgctgg
     1801 atggtgccgc acgtcataac gtacaggtcc tgataaccga cagcgggcag cgaaccggta
     1861 caggcagtgc gctgatggcc atgaaggatg ccggggtgaa cacatatcgc tggcagggcg
     1921 gagaacagcg accggccacc atcatcagtg aaccggaccg taatgtccgc tatgcccggc
     1981 tggccggaga ttttgcggcc agcgtgaaag ccggagaaga gagcgtggca caggtcagcg
     2041 gggtacggga acaggccata ctgacacagg ccattcgcag tgagctgaaa acgcagggcg
     2101 tgctcggaca ccctgaggtg accatgactg cactgtcacc ggtctggctg gacagccgga
     2161 gccgttatct gcgggatatg taccgaccgg ggatggtgat ggagcagtgg aacccggaga
     2221 cacgcagtca tgaccgctat gttatcgacc gggtgacggc gcagagtcac agcctgaccc
     2281 tgcgggatgc gcagggcgaa acgcaggtgg tgcgtatttc ctccctggac agcagctggt
     2341 cgctgttccg gccggaaaaa atgccggtgg cagacggcga gcgactgagg gtgacaggga
     2401 aaattcccgg actccgcgtc tccggcggtg accgcctgca ggtggcatcc gtcagtgaag
     2461 atgcgatgac ggttgttgtg ccggggcggg ctgaaccggc caccctgcct gtggctgatt
     2521 caccgttcac ggcactgaag ctggagaacg gctgggtgga aacgcccggg cattccgtca
     2581 gtgacagtgc gacggtgttt gcctccgtca cacagatggc aatggacaac gccaccctga
     2641 acggtctggc ccgcagcggt cgtgatgtcc ggctgtattc ctcactggat gaaacccgta
     2701 ctgcggaaaa actcgcccgc catccgtcct ttacggtggt ttctgagcag ataaaggcgc
     2761 gtgccggtga gacattgctg gaaaccgcta tcagtctgca gaaagccggg cttcacacgc
     2821 cggcacagca ggccattcat ctggcccttc ctgtgctgga aagtaaaaac ctggccttca
     2881 gcatggtgga cctgctgaca gaggcgaagt cgtttgctgc agaaggaacc ggttttactg
     2941 aactgggagg ggaaatcaat gcgcagataa aacgcggtga cttactgtat gtggatgtgg
     3001 caaaaggcta tggcacaggc ctgctggttt cccgtgcgtc gtatgaggca gaaaagagca
     3061 ttcttcgcca tattctcgaa ggcaaggagg cggtcacgcc gctgatggag agagtacccg
     3121 gcgaactcat ggagacgtta acgtcgggac agcgtgccgc cacccgtatg atactggaaa
     3181 cgtccgaccg tttcacggtg gtacaggggt atgccggtgt gggtaagacc actcagttcc
     3241 gggcggtgat gtcagccgtg aacatgctgc cggagagtga gcgtccccgg gttgtggggc
     3301 tggggcccac gcaccgtgcg gtcggtgaga tgcgcagcgc cggcgtggat gcacagacac
     3361 tggcgtcctt tctgcatgac acgcagctgc agcagcgcag cggagaaacg ccggatttca
     3421 gcaacacgct gtttctgctc gatgagagct caatggtggg caataccgac atggcacggg
     3481 catacgccct gattgcggcc ggtggcggtc gtgctgtggc cagcggtgac acggaccagc
     3541 tgcaggccat cgcgcccggt cagcctttcc gtctccagca gacgcgcagt gctgccgatg
     3601 tggccatcat gaaggagatt gtgcgtcaga cgccggaact gcgggaggcg gtatacagcc
     3661 tgattaaccg ggatgtggaa agggcactgt ccgggcttga gagtgtgaaa ccgtctcagg
     3721 tgccacgtct ggagggcgca tgggcaccgg agcactccgt gacggagttc agtcacagcc
     3781 aggaagcgaa actggcagaa gcgcagcaga aggcgatgct gaaaggcgag gctttcccgg
     3841 atatccccat gacactgtat gaggccattg tccgcgacta taccggcagg acaccggaag
     3901 cacgggagca gacgctgatt gtcacgcacc tgaatgagga ccggcgcgta ctgaacagca
     3961 tgattcatga tgcacgggaa aaggccggtg agctgggcaa agagcaggtc atggtgcctg
     4021 tcctgaacac agcgaatata cgtgacggag aactgcgtcg tctctccacc tgggagaaaa
     4081 acccggatgc ccttgccctg gtggatagtg tgtatcaccg gattgccggt atcagcaagg
     4141 atgacgggct gataaccctg caggatgcgg aaggtaacac gcggctgatt tcgccccggg
     4201 aggcggtggc tgaaggtgtc acactgtaca ccccggacaa aatccgggtg gggaccggcg
     4261 accggatgcg cttcacgaag agtgaccggg agcgcggtta tgtggccaac agcgtctgga
     4321 cggtgacagc ggtttccggt gacagtgtca cgctgtcgga cggacagcag acccgggtga
     4381 ttcgccccgg tcaggagcgg gcagagcaac atattgacct ggcctatgcc atcactgccc
     4441 acggtgcgca gggggcgagt gaaacctttg ccatcgcgct tgaaggcacg gaaggtaacc
     4501 ggaaacagat ggccggcttt gagtcagcct acgtggccct gtcgcgtatg aagcagcatg
     4561 tgcaggtgta caccgataac cgtcagggct ggacggatgc cattaacaat gccgtacaga
     4621 aaggaactgc ccacgatgtg ctggagccga aaccggaccg ggaggtcatg aatgcgcagc
     4681 ggctgttcag tacggcacgg gaactgcggg acgtggcggc aggccgtgcc gttctccgtc
     4741 aggcggggct ggccggggga gacagtccgg cacggtttat tgcaccgggg cgcaaatatc
     4801 cgcagccgta tgtggcactg cctgcttttg accgtaacgg caagtccgcc ggtatctggc
     4861 tgaacccgct gaccacggat gacggaaacg ggctgcgggg attcagcggt gaaggacggg
     4921 tgaaaggcag cggggatgcg cagtttgtgg ccctgcaggg cagccgtaac ggcgagagcc
     4981 tgctggctga taatatgcag gatggtgtcc ggattgcccg tgataacccc gacagcggtg
     5041 tggtggtgag aatcgccggt gaaggtcgtc cgtggaatcc cggtgccatc accggtggtc
     5101 gcgtgtgggg ggatatcccg gacaacagcg ttcagccggg agccggaaat ggcgaaccgg
     5161 tcacggcaga ggtactggca cagcggcagg ctgaagaggc catccgccgt gaaacggaac
     5221 gtcgcgcaga tgaaattgtc cgtaaaatgg cagagaacaa acctgacctg ccggacggca
     5281 aaacagagca ggctgtcagg gagattgccg gacaggagcg tgaccggact gccacttctg
     5341 aacgggaagc cgcgctgccg gagagtgtgc tgcgtgaatc acaacgggag cgggaagcgg
     5401 tccgggaggt tgcccgggaa aatctgctgc aggagcgtct gcagcagatg gagcgggata
     5461 tggttcgtga cctgcagaaa gagaaaaccc tgggcggaga ctgatacagg aagataaacg
     5521 ctgatgacaa cggataacac gaacacgaca cgtaacgatt cactggctgc acggaccgat
     5581 acctggttgc agtcatttct ggtctggtca cccggacagc gggatatcat caaaacggtg
     5641 gcactggtgc tgatggtact ggaccacatc aatctgatat tccagctgaa gcaggaatgg
     5701 atgtttctgg ccgggcgagg agcctttccg ctgtttgccc tggtgtgggg gctgaatctg
     5761 tcccgtcatg cgcatatccg gcaaccggcc attaaccggc tgtggggatg ggggattatt
     5821 gcccagttcg catattatct ggcaggcttt ccctggtatg aggggaatat cctgtttgcc
     5881 tttgcagtgg cggcacaggt gctgacgtgg tgtgaaacgc gcagtgggtg gcgtacagca
     5941 gcggccattc ttctgatggc cctgtggggg cctttgtccg gcaccagtta cggcattgcc
     6001 gggctgctga tgctggcagt cagccaccgg ctgtaccggg cggaagacag agcggaacgt
     6061 ctggcgctgg tcgcctgtct gctggctgtt attccggcgc ttaaccttgc caccagtgat
     6121 gcagcggcgg tggctggtct tgtgatgacg gtgctgaccg ttggtctggt gtcgtgtgca
     6181 ggaaaatcat taccccgttt ctggcccggg gattttttcc cggtattcta cgcctgtcat
     6241 ctggctgtgc tgggcgttct ggcgctgtga cgggtgtggt atctttgacc gtaacaggat
     6301 gattcgtcag aggcagaaca cagcatgaca gagcagaagc gaccggtact gacactgaag
     6361 cgaaaaacgg aaggggagac gcctgtccgc aggcggaaaa ccatcatcaa tgtcaccacg
     6421 ccaccaaagt ggaaggtgaa aaagcagaag ctggccgaga aagcagcccg ggaagcggag
     6481 ctggcggcaa aaaaagcgca ggccagacag gcgctgtcca tttatctgaa cctgccctcg
     6541 ctggatgagg ccgtgaacac cctgaaaccc tggtggccgg gattatttga cggtgaaacg
     6601 ccccggcttc tggcctgcgg tatccgggac gtgttactgg aagacgtggc gcagcggaat
     6661 atcccgctct cgcataaaaa actgcgcagg gcgctgaagg ccatcacccg ttcagaaagc
     6721 tatctgtgtg ccatgaaagc cggtgcctgc cggtatgata cggaagggta tgtgacggag
     6781 catatttctc aggaggagga agcgtatgcg gcagcgcgtc tggataaaat ccgccgccag
     6841 aacaggataa aggcagagct tcaggccgtg cttgatgaga aataaaaaaa agcctccatc
     6901 agagaggagg cagggaaata aggcttaaaa ggaaataact tccgtacccg aaaggaagcc
     6961 aattcagtag attatttaac accataatac tgacgggtta aagaggagtg ataaagatga
     7021 atggattcag aaacagttca cggaacggtc aggtctggcg ctggcagcgt gccggaagcc
     7081 gggccgttat tctggaggtc agtggacgct ggatggaagc ggcagaagca tggcgacggg
     7141 ctgccggtgt tgcgccccgg acagactggc agcagtttgc ccgaaaaagg gctgaacact
     7201 gccaccggcg cagccgggga agggtgtaac gaaaaaagca gccttcatta ctccagggat
     7261 tcagaaggct gaataatgca tgagatttgc tttttttaat atccggtcgg gcagtatgac
     7321 cggacagttc tgagtagcac agagtttttt ccgtattaag tcgtgatttt atattttgtg
     7381 attaatttca caaaataagg tgttattcag tgtgtgctgc gatattcagg attacctgaa
     7441 atacaggtgc catttatctg atatggagaa taacatgagg aaatatattc cactggtatt
     7501 atttatcttt tcatggccgg tattgtgtgc agatattcat ggacgggttg ttcgtgtact
     7561 ggacggtgat accatcgagg tcatggactc acggaaggca gtgcggatca ggctggtaaa
     7621 tattgatgca ccggaaaaga aacaggatta tggccggtgg tcaacggaca tgatgaaatc
     7681 actggttgca ggaaaaacgg ttaccgtcac atattttcag cgagatcgtt atggtcgtat
     7741 tctcgggcag gtttatgcac cagacggaat gaatgttaat cagtttatgg tccgggccgg
     7801 ggctgcctgg gtatatgagc agtacaacac agatcccgtg ctgcctgtcc ttcagaacga
     7861 agccagacaa caaaaacgtg ggctctggtc agatgctgat ccggttcccc cctggatatg
     7921 gagacaccgt aaataaagcg atggcaatct tatcattcat ctgttctcag cgggaataaa
     7981 aatggcgaaa acaaaacagg aatggctgta tcagttacgg cgatgttcat cagtcaatac
     8041 gctggaaaaa attattcata aaaacagaga cagtttatta aacagtgaac gtgaatcatt
     8101 taattcagca gcagaccacc ggttagcgga attaatcacc ggaaagttat atgacaggat
     8161 accaaaagag atatggaaat atgtaaggta ataattcaga ataattaatt accaggagca
     8221 gataaacaat gaagctgatt atttttattc tcattgttct gattattgca gcgttactta
     8281 tccggattat cctcaaaagt gtaaatcagc attctccttt gctgatgcag ttgcatgcgg
     8341 caggtatacg gaccggtgat gcagagcgaa tactgtccgg cggtgaatac tggcaacgtc
     8401 agaagacgct gctgacagaa agggaagtca gttttatgaa aggactgttc agaattgtgg
     8461 atatgaagcg gtggtatctg tgtccgcagg tacgggtcgc ggatatcgtc cagctgaacg
     8521 ggaatatccg gccacgatca cgccagtggt ggcagttatt caggatggtg tctcagtggc
     8581 atgttgatgt ggttatcgtt gaacggcgtt cgttcagtat tgttgcagca gtagagctgg
     8641 atgatgccag ccatttacga ccggaacgcc ggcgccggga tattcttctg gaagaggttc
     8701 tgaggcaggc tggtattccg ttgctcagaa gccacgatgc cagaaaactg ctgcagatga
     8761 ccggagaatg gctgaataca acaggggctg atcagcagtc cccggaacat cgtagctgac
     8821 gccttcgcgt tgctcagttg tccaaccccg gaaacgggaa aaagcaagtt ttccccgctc
     8881 ccggcgtttc tgtaataaaa agcgggaaat ggtggtcaca gagtaaattc cggaaccacc
     8941 tgtacccaaa ctttatataa taatggtgaa tatatgaaat atgatgatgt atcagaattg
     9001 atgattaagc gtgaacacaa tattcttatt gttgggaact ctgcctccca cattgataag
     9061 ttttttaagg ggtattctat accaggttct aaatattatg atttcactca gattaacagt
     9121 gattcagatg tagagagaaa tgaggatgct gtctccttta tcagggatgc aatgaatagt
     9181 gaagagcaga caatcatttt taattgtgct gggtggcctg atttaggtgg tggatcagca
     9241 gtatcacagt ttgcaatgat ggtcagaaaa tttggtaaac aacttattgt cgctgtaagt
     9301 gaaaaggatg ccatcaaatt aaaagacaac tttgatatta ttgggatgtt gtcatatggt
     9361 aaagaaaatt ttattgccat gagtcatacg aaaaacgagt taactggagg aaaacgtcgg
     9421 taccggatta aaaatggatg taaataaaaa gcatacttat tggctggagt gacgccttcg
     9481 tgcttctgta gtaaaaagcg ggcaatgcag gtaatagtca gcaacaggga atgtggtatt
     9541 atcgcggcgg gtgtctgagc ctttctggtt caggcaagac gcaggtacca gaaatgcaaa
     9601 gaccccactt gttaatccat taactcgtga ggtctgcatg aagtacattg cactactgat
     9661 tgtagcctct tccttgcaga gaggtcaaag tttatgacga agtatgccct tatcgggttg
     9721 ctcgccgtgt gcgccacggt gttgtgtttt tcactgatat tcagggaacg gttatgtgag
     9781 ctgaatattc acaggggaaa tacagtggtg caggtaactc tggcctacga agcacggaag
     9841 taaactgccg ggcggggacg gaagtccccg ctttccggaa gtgtgaggta tttcaggggc
     9901 agacacccga catgccagaa acagccggtc ccgcccgggg ccggctcccg ggttaaggca
     9961 tttcctgttt tttcagccat ttcgcaacaa aaatcacatt aaacaacagt aatccccgtt
    10021 gatttgtgcg ccaacacaga tcttcgtcac aattctcaag tcgctgattt caaaaaactg
    10081 tagtatcctc tgcgaaacga tccctgtttg agtattgagg aggcgagatg tcgcagacag
    10141 aaaatgcagt gacttcctca tcaggtacaa agcgagcata cagaaaaggt aatcctctga
    10201 cgcttgcaga acgtcagcag gcatccctgg caagaaaacg agctacacat aaagaactca
    10261 gagtgttcat tcctgctgcg ttaaaagcgc aattgcaggt gatgtgtgaa gctgagggcg
    10321 ttacccaggc tgagatgatt gccgaactga taaagcagaa gagtgctttt agctaactaa
    10381 aatgtagcga catcacattc ttgcctgttt ctggtgtgcg tgatagattg ctgatcgttt
    10441 aaggaatttt gtggctggcc acgccgtaag gtggcaggga actggttctg atgtggattt
    10501 acaggagcca gaaaagtgaa aaccccgata atcttcgaca agtttggcga ctgagaagat
    10561 taccggggcc atctaaaaac cgtatagcca acaattcagc tatgcgggga gtatagttat
    10621 atgcccggaa aagttcaaga cttctttctg tgctcactcc ttctgcgcat tgtaagtgca
    10681 ggatggtgtg actgatcttc aacaaacgta ttaccgccag gtaaagaacc cgaatccggt
    10741 gtttacaccc cgtaaaggtg ccggaacgct gaagttctgc gaaaaactga tggaaaaggc
    10801 ggtgggcttc acttcccgtt ttgatttcgc cattcatgtg gcgcatgccc gttcgcgtgg
    10861 tctgcgtcga cgcatgccac cggtgctgcg tcgacgggct attgatgcgc tcctgcaggg
    10921 gctgtgtttc cactatgacc cgctggccaa ccgcgtccag tgctccatca ccacgctggc
    10981 cattgagtgc ggactggcga cggagtctgc tgccggaaaa ctctccatca cccgtgccac
    11041 ccgggccctg acgttcctgt cagagctggg actgattacc taccagacgg aatatgaccc
    11101 gcttatcggg tgctacattc cgaccgatat cacgttcaca tctgcactgt ttgctgccct
    11161 cgatgtatca gaggaggcag cggccgccgc gcgccgcagc cgtgtggtat gggaaaacaa
    11221 acaacgcaaa aagcaggggc tggataccct gggcatggat gaactgatag cgaaagcctg
    11281 gcgttttgtt cgtgagcgtt ttcgcagtta tcagacagag cttaagtccc ggggaataaa
    11341 gcgtgcccgt gcgcgtcgtg atgcggacag ggaacgtcag gatattgtca ccctggtgaa
    11401 acggcagctg acgcgcgaaa tcgcggaagg gcgcttcact gccaatcgtg aggcggtaaa
    11461 acgcgaagtt gagcgtcgtg tgaaggagcg catgattctg tcacgtaacc gtaattacag
    11521 ccggctggcc acagcttccc cctgaaagtg acctcctctg aataatccgg cccgcgccgg
    11581 aggcattcgc acgcctgaag cccgccggtg cacaaaaaaa cagcgtcgca tgcaaaaaac
    11641 aatctcatca tccaccttct ggagcatccg attccccctg tttttaatac aaaatacgcc
    11701 tcagcgacgg ggaattttgc ttatccacat ttaactgcaa gggacttccc cataaggtta
    11761 caaccgttca tgtcataaag cgccagccgc cagtcttaca gggtgcaatg tatcttttaa
    11821 acacctgttt atatctcctt taaactactt aattacattc atttaaaaag aaaacctatt
    11881 cactgcctgt cctgtggaca gacagatatg cacctcccac cgcaagcggc gggccccgac
    11941 cggagccact ttagttacaa cacacaaaaa caacctccag aaaaaccccg gtccagcgca
    12001 gaaccgaaac cacaaagccc ctccctcata actgaaaagc ggccccgccc cggcccaaag
    12061 ggccggaaca gagtcgcttt taattatgaa tgttgtaact acatcttcat cgctgtcagt
    12121 cttctcgctg gaagttctca gtacacgctc gtaagcggcc ctcacggccc gctaacgcgg
    12181 agatacgccc cgacttcggg taaaccctcg tcgggaccac tccgaccgcg cacagaagct
    12241 ctctcatggc tgaaagcggg tatggtctgg cagggctggg gatgggtaag gtgaaatcta
    12301 tcaatcagta ccggcttacg ccgggcttcg gcggttttac tcctgtatca tatgaaacaa
    12361 cagagtgccg ccttccatgc cgctgatgcg gcatatcctg gtaacgatat ctgaattgtt
    12421 atacatgtgt atatacgtgg taatgacaaa aataggacaa gttaaaaatt tacaggcgat
    12481 gcaatgattc aaacacgtaa tcaatatctg cagtttatgc tggttatgct ggctgcctgg
    12541 ggcattagtt ggggagccag atttgtcatg gagcaggccg ttctgcttta tggatcagga
    12601 aaaaactatt tgttcttcag tcatggtact gttctgatgt acctgctgtg tgttttcctg
    12661 gtataccgcc gttggatagc tccgctaccg gtcgttggtc agctgcgcaa cgttggcgta
    12721 ccgtggctgg tcggtgcgat ggccgtggtg tatgtcggtg tatttctgct cggtaaggcg
    12781 ctggctctgc ctgctgagcc atttatgacg aaactttttg ccgataagtc catacctgac
    12841 gtgatcctga cgttgctgac catctttatc cttgccccgt tgaatgagga aacgctgttc
    12901 cgggggatta tgctgaacgt cttccgttca cggtactgct ggacgatgtg gctgggggcg
    12961 ctgataacgt cgttgttgtt cgtcgccgcg cacagccagt atcagaacct gctgacactg
    13021 gcagaactgt tcctggtggg gttgattaca tcagtggcca ggatcagaag tggtggcctg
    13081 ctgctgccgg tattgctgca tatggaagca accacgctgg gtttactgtt tggttgaaag
    13141 ttatattttt attaaacatt gtgcgttaaa gcctggtgtg tttttttagt ggatgttata
    13201 tttaaatata acttttatgg aggtgaagaa tgcataccac ccgactgaag agggttggcg
    13261 gctcagttat gctgaccgtc ccaccggcac tgctgaatgc gctgtctctg ggcacagata
    13321 atgaagttgg catggtcatt gataatggcc ggctgattgt tgagccgtac agacgcccgc
    13381 aatattcact ggctgagcta ctggcacagt gtgatccgaa tgctgaaata tcagctgaag
    13441 aacgagaatg gctggatgca ccggcgactg gtcaggagga aatctgacat ggaaagaggg
    13501 gaaatctggc ttgtctcgct tgatcctacc gcaggtcatg agcagcaggg aacgcggccg
    13561 gtgctgattg tcacaccggc ggcctttaat cgcgtgaccc gcctgcctgt tgttgtgccc
    13621 gtaaccagcg gaggcaattt tgcccgcact gccggctttg cggtgtcgtt ggatggtgtt
    13681 ggcatacgta ccacaggtgt tgtacgttgc gatcaacccc ggacaattga tatgaaagca
    13741 cggggcggaa aacgactcga acgggttccg gagactatca tgaacgaagt tcttggccgc
    13801 ctgtccacta ttctgacttg aacatggggt ttgaggggca actggatgaa aacgtacgat
    13861 ttagggcact aaaaccgctg ttgtcccacc attctggtga ttcccaaacg ttatttggct
    13921 aaaaagtagt tttgatgtgg tttattttcc gaattccaag cgcagcccta ctttcttgtg
    13981 cgtatttgcg tgttttgcgc agttttgaag ttcctacatc agattgactt cggatacaaa
    14041 ttcaatgaac attgatgcaa atactgacct atcagtttct taactaaata ctcgagatac
    14101 atctaatggc taatgaaaac ttagatattg actccaagac agcagaagaa ttatacgagt
    14161 ggtatctgga tgataaatta attataaatc gaagatacca aagaaaatta gtatggtccc
    14221 tcgaagagaa aacagcactg atttcatcaa tgactcagca ataccctatc cctttgctat
    14281 tgtttgtttc cattgacaac aaaagagaaa tccttgatgg aatgcaaaga ctagaagctt
    14341 taatgagttt tattgagcag cgattttcat taaatggtaa atactttaat ttagatgcca
    14401 ttgcattaac aaaacaacta aaagacagtg gtaaacttca acaaaaagag ccagtgctat
    14461 cccgcgaaag ttctacatta attgctcgct atagatttgc tatatctgaa tactcttcaa
    14521 cagaggacca cattgacgaa gtgtttcgac gcataaattc caatggtaaa attttatcaa
    14581 aacaagaact acgaagtgcg gggtgcgtta gtaatttttc agagttagta aggaaaatat
    14641 cgacaattat aagaggagat actacacata gcgatataat gggactgaat aaaatacata
    14701 acatatcaat ttgcaatgat ggactcgatt atggaataaa tatagacaat catttctata
    14761 ttagaaatca tatcattagt aggcctagca ttagagactc tgacgatgaa gagttagttg
    14821 caaatatctt gggctatata tttttagatg ataagccaac atcaggatca acatcgctcg
    14881 atacatttta cggtgaaggt tcgacgagtc atgcaatcca tacacgaact caattagaga
    14941 attacattca gacaaatggt gcagataaaa ttgtcaataa ttacctcttc gtttatgaga
    15001 tgattcaaaa attatttgat gcaaataatt tgaactttag atcacacatt ctgggcaatg
    15061 catcttcatc tcaagaatgt cctcgttact atcaagctgt ttttcttgct ttgtatgaat
    15121 taatcataaa tgaaaacatg caacttgacg atgaacagaa atttatcgct cagttaggag
    15181 acagtgtaca acgttcaatg gtgcaaaccg aagggggccg ttgggcagct agcgctcgtc
    15241 agaaaagtgt cgaagattta tgtgctttaa tcagacgata ttttaaagaa tcagaaaata
    15301 aatttatcaa tcatgcttgg caaacactga taagaactct acttaataac tcaagaacag
    15361 aacaaccaaa ttatgatttt aaacagggag gtgatgctgc caacttactg atttagtgta
    15421 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca
    15481 gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctatctctgc
    15541 tctcaggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    15601 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    15661 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    15721 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    15781 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    15841 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    15901 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    15961 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
    16021 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    16081 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    16141 gtcagaagta agttggcagc agtgttatca ctcatggtta tggcagcact gcataattct
    16201 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    16261 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
    16321 accgcaccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    16381 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    16441 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    16501 caaaatgccg caaaaaaggg aataagggcg acacgaaaat gttgaatact catactcttc
    16561 ctttttcaat attattgaag catttaccag ggttattgtc tcatgagcgg atacatattt
    16621 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    16681 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
    16741 aggccctttc gtcttcaaga attttataaa ccgtggagcg ggcaatactg agctgatgag
    16801 caatttccgt tgcaccagtg cccttctgat gaagcgtcag cacgacgttc ctgtccacgg
    16861 tacgcctgcg gccaaatttg attcctttca gctttgcttc ctgtcggccc tcattcgtgc
    16921 gttctaggat cctccggcgt tcagcctgtg ccacagccga caggatggtg accaccattt
    16981 gccccatatc accgtcggta ctgatcccgt catcaatgaa ccggactgcc acgccctgag
    17041 cgtcaaattc ctttatcagt tggatcatat cggcagtgtc gcggccaaga cggtcgagct
    17101 tcttaaccag aatgacatca ccttcctcca ccttcatcct cagcaaatcc agcccttccc
    17161 ggtctgtt
//
LOCUS       DAETQN010000091        17144 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.091, whole genome shotgun sequence.
ACCESSION   DAETQN010000091 DAETQN010000000
VERSION     DAETQN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17144
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.091"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="K0W68_002990"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="K0W68_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBI3074496.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="K0W68_002991"
     CDS             2219..2767
                     /locus_tag="K0W68_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBI3074497.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="K0W68_002992"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="K0W68_002992"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBI3074498.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="K0W68_002993"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="K0W68_002993"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBI3074499.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="K0W68_002994"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="K0W68_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBI3074500.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="K0W68_002995"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="K0W68_002995"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBI3074501.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8301..9503)
                     /gene="pncB"
                     /locus_tag="K0W68_002996"
     CDS             complement(8301..9503)
                     /gene="pncB"
                     /locus_tag="K0W68_002996"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBI3074502.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9769..12381
                     /gene="pepN"
                     /locus_tag="K0W68_002997"
     CDS             9769..12381
                     /gene="pepN"
                     /locus_tag="K0W68_002997"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBI3074503.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="K0W68_002998"
     CDS             complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="K0W68_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBI3074504.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13352..14143)
                     /gene="ssuC"
                     /locus_tag="K0W68_002999"
     CDS             complement(13352..14143)
                     /gene="ssuC"
                     /locus_tag="K0W68_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBI3074505.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14155..15302)
                     /gene="ssuD"
                     /locus_tag="K0W68_003000"
                     /pseudo
     CDS             complement(14155..15302)
                     /gene="ssuD"
                     /locus_tag="K0W68_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15299..16258)
                     /gene="ssuA"
                     /locus_tag="K0W68_003001"
     CDS             complement(15299..16258)
                     /gene="ssuA"
                     /locus_tag="K0W68_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBI3074506.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            complement(16251..16826)
                     /gene="ssuE"
                     /locus_tag="K0W68_003002"
     CDS             complement(16251..16826)
                     /gene="ssuE"
                     /locus_tag="K0W68_003002"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBI3074507.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
BASE COUNT         4299 a         4421 c         4350 g         4074 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcagtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcggggttg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcctcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tggggggggg gatacctatg ttacctggca tctgcaatca
     8221 gacaagcaga attcgcaaat gcagcgaaag atttcggaat taaaggtaaa aaaagggagc
     8281 cgattagctc cctgatgata ttaactggct tttttaatat gcggaaggtc gaacgctttg
     8341 cgcagcgccc gaacaaacgc tttatcatgg cagatagttt tgccagggct gtcagaaagt
     8401 ttcgccaccg gtttaccgtt acactctacc aacttaatca caatattcag gggttttacc
     8461 tgggggatat cgcaggtcag gcgagtccca ataccaaaac ttaattgcac gcgggaagag
     8521 aagtggcggt atagctcaac cgctttgcgt aaatccagat tgtcagagaa aaccagcgtt
     8581 ttactctgtg gatcaattcc cagcttttca taatgtgcaa tggctttttc accccattca
     8641 accgggtcgc cagagtcatg acgcaggccc tgataccgac tagcgaactc gacaccgaaa
     8701 tcacgcagga aagcatccat agtgatgcag tcggttaatg caatgccaag ttggtcggga
     8761 tactcttcca gccaggcagc aagtgcagct cgctggctgt tggctagatc cgggctgatt
     8821 tgctgatgtg cctggaacca ttcgtgtgcc tgtgttccca tcggcgtgag ggaaagccga
     8881 cgcgccagat cgtagttgct ggtgcccacg aaccaggatt cctgttgcag acgcttaacg
     8941 atggtttctt gtacttcgcg agaaaaacgg cgacgggtgc caaaatccat cagatggaag
     9001 cgcgacatat caagaccggc ggttaacgcc gagaagtcga ctaatttgct ttccagcgtg
     9061 tcgagggctt gcgcaacgtc ggcctgcggt gagcgatagc gatgtaccat ttcactgata
     9121 accgccagca aaggaacttc ccagaggatg acttcacgcc acggaccgct taagcgaata
     9181 tccagcttgc cattatcgtt ggacacggtg acttgttccg ggttaaagcg gaactcgcgt
     9241 aaccagttaa gatagtcggc cttaaagaaa ggcagggcag agagccactg atattcatca
     9301 tcctgcaggc gcaggtgctg catcgcctga atctgttcac gaatagcatc ggcataaata
     9361 cccagcagat cgtcacctcg gcaacgaaac tccgccgcga catgcacatc gtaatagtga
     9421 tgaaacacgg cttgctgcat atgcaactta taagcatctg tatccagcaa cgagtgcaga
     9481 acaggagaag cgaattgtgt cataggtgcg cagtagcgtc ctcgtacagg agcgtttagt
     9541 acaataaaca tcttcaggaa ctgctggagt ataccttgtt tcgcaattta ttgaaccccg
     9601 atcacaccat atgccacctt tctggtcgat ggcattacgc ggtgtatgtt ataaaaatgt
     9661 agcaataaag gcgtttgtac ctgaaaagat gaagattctg catagcgcga tttacgcaac
     9721 aggaatagac tgaacaccag actctataaa agatgctaaa ggttatttat gactcaacag
     9781 ccacaagcca aataccgtca cgattatcgt gcgccagatt accagattac tgatattgac
     9841 ttgacctttg acctcgacgc gcaaaagacg gtcgttaccg cggtcagcca ggctgtccgt
     9901 catggtgcat cagatgctcc acttcgtctc aacggcgaag acctcaaact ggtttctgtt
     9961 catattaatg atgagccgtg gaccgcctgg aaagaagaag agggcgcact ggtcatcagt
    10021 aatttgccgg agcattttac gcttaagatc gttaatgaaa taagcccggc ggcgaatacc
    10081 gcgctggaag ggctttatca gtcaggcgat gcgctttgca cccagtgtga agccgaaggt
    10141 ttccgccata ttacgtatta tctcgaccgc ccggacgtgc tggcgcgttt taccaccaaa
    10201 attattgccg ataaaaccaa atatcccttc ctgctttcca acggtaaccg cgttgcgcaa
    10261 ggtgaactgg aaaacggacg ccattgggta cagtggcagg acccgttccc gaaaccgtgc
    10321 tacctgtttg cgctggtggc aggcgacttt gatgtactgc gcgacacctt taccacgcgt
    10381 tctggtcgcg aagtggcgct ggagctgtac gtcgatcgcg gcaaccttga tcgcgcgccg
    10441 tgggcgatga cctcgctgaa aaactcaatg aaatgggatg aagaacgctt cggcctggag
    10501 tatgacctcg acatctatat gatcgtcgcg gtggatttct tcaatatggg cgcaatggag
    10561 aataagggtc tgaatatctt taactccaaa tatgtgctgg cccgcaccga caccgccacc
    10621 gacaaagatt acctcgatat tgaacgcgtt atcggccatg aatatttcca taactggacc
    10681 ggtaaccgag tgacctgccg cgactggttc cagctcagcc tgaaagaagg tttaaccgtc
    10741 ttccgcgatc aggaattcag ctctgacctt ggttcccgcg cagttaaccg catcaataat
    10801 gtgcgcacca tgcgcggatt gcagtttgcg gaagacgcca gcccgatggc gcacccaatc
    10861 cgcccggaca tggtcattga gatgaacaac ttctacaccc tgaccgttta cgagaagggc
    10921 gcggaagtga tccgtatgat ccacaccctg ctgggcgaag aaaacttcca gaaagggatg
    10981 caactctatt tcgagcgtca tgatggtagc gcagcgacct gtgacgactt tgtgcaggcg
    11041 atggaagatg cgtcgaatgt tgatctctcc catttccgcc gttggtacag ccagtccggt
    11101 acgccgattg tgaccgtcaa agacgactac aatccggaaa ctgagcagta caccctgacc
    11161 atcagccagc gcacgccagc cacgccggat caggcagaaa aacagccgct gcatattccg
    11221 tttgccatcg aattgtatga caacgaaggc aaagtgatcc cgttgcagaa aggcggtcat
    11281 ccggtgaatt ccgtgctgaa cgtcacccag gcggaacaga cctttgtttt tgataatgtc
    11341 tacttccagc cggtgcctgc gctgctgtgc gaattctctg cgccagtgaa actggaatat
    11401 aagtggagtg atcagcaact gaccttcctg atgcgtcatg cgcgtaatga tttctcccgt
    11461 tgggatgcgg cgcaaagttt gctggcaacc tacatcaagc tgaacgtcgc ccgtcatcag
    11521 caagggcagc cgctgtctct gccggtacat gtggctgacg ctttccgcgc ggtgctgctc
    11581 gatgagaaga ttgatccggc gctggcggca gaaatcctga cgctgccttc tgtcaatgaa
    11641 atggctgaac tgttcgatat catcgacccg attgctattg ccgaagtacg cgaagcactc
    11701 actcgtactc tggcgactga actggcggat gagctgctgg ctatttacaa cgcgaattac
    11761 cagagcgagt accgtgttga gcatgaagat attgcaaaac gcactctgcg aaatgcctgc
    11821 ctgcgcttcc ttgcttttgg tgaaacgcat ctggctgacg tgctggtgag caagcaatac
    11881 catgaagcaa acaatatgac cgatgcgctg gcagcgcttt ctgcggcggt tgctgcacag
    11941 ctgccttgcc gtgacgcgct gatgcaggag tacgacgata agtggcatca ggacggtctg
    12001 gtgatggata aatggtttat cctgcaagcc accagcccgg cggcgaatgt gctggagacg
    12061 gtgcgtggtc tgttgcagca tcgctcattt accatgagca acccgaaccg tattcgttcg
    12121 ttgattggtg cgtttgcggg cagcaacccg gcagcgttcc atgccgaaga tggcagcggt
    12181 taccagttcc tggaggaaat gcttaccgac ctcaacagcc gtaacccgca ggtagcttca
    12241 cgtctgattg aaccgctgat tcgcctgaaa cgttatgatg ccaaacgtca ggagaaaatg
    12301 cgcgcggcgc tggaacagtt gaaagggctg gaaaatctct ctggcgatct gtacgagaag
    12361 atcaccaaag cactggcttg atacttttga gccaaaatgc ctgatgcgac gcttgacgcg
    12421 tcttatcagg cctacaactc cgttgaaatt gctggttttg taggctggat aaggcgttca
    12481 cgccgcatcc ggcagccgtg cccaactgcc tgatgcgatg cttgacgtgt cttatcaggc
    12541 ctacaagtcc gttgaaaatg ctggttttgt gcgccacatc cggcatatta accatgacgc
    12601 cgaatcagtt gttcagaatg cccacgccgc attacccgtt gcaatacttc cgcttccagc
    12661 tccgctaatc gcaccgaccc taatcgacgc ggacggggga tatccactgt caaatccaga
    12721 ccaatttttc cctcttcaat taacaacacc cggtcagcca ttgctaccgc ttcgctcaca
    12781 tcatgcgtca ccaacagtac ggtaaagccg tgctgctgcc aaagtgacac aatcaaatcc
    12841 tgcatctcga gtcgcgttaa ggcgtccagt gccccgagcg gttcatcaag caacaataaa
    12901 cccggtcgat gaatcaacgc tcttgccagc gccacacgct gtttctgccc gccagaaagt
    12961 gcggcaggcc attccccggc gcgattctcc agtcctactg cagccaacgc ccgacgcgcg
    13021 gcatcgcgcc actggccttt aaggcctaac ccaacgttat caatcaccga tttccacggc
    13081 agcagacgcg catcctgaaa catcattcgc gtgtcttcct gaatctcagc cagcggtgtg
    13141 gtgcccgcta acacatcgcc tgcggttggc gtttccagac ctgccagcag gcgcagcagg
    13201 gtacttttgc caccaccgct gcggcccacc accgccacaa actgacctgc cggaatatgt
    13261 aaatccagtt ggttcaggac gatattttcc gcgtaatgtt tgcttactgc attgagcaac
    13321 aatggtgtgc cctggttcag acgagcagta ttcataccgt ggcctccttc aaatgataag
    13381 ccgggttcca gcgcaaccag aggcgctcta acagctgcgc gctgacgtca gcaagtttgc
    13441 cgagcagggc gtaaagaata atagcgacta ccaccacgtc cgtttgcaaa aactcccgcg
    13501 cattcatcgc cagataacca atgcctgaat tggcagaaat ggtttcggca acaatcagcg
    13561 tcagccacat caggcccaac gcaaaacgca cgccgaccat aattgagggc agggcaccag
    13621 gcaggatcac atggataaac agcggtatgc cggataatcc atagctacgc gccatttcca
    13681 ccagaccgcg atcgatatta cggatgccat gccaggtgtt gatataaatg gggaatagcg
    13741 tgccgagcgc caccagaaag atttttgcgg attcatcaat gccaaaccac aaaatcacca
    13801 gcgggatcag cgccagatgc ggtacgttgc gcaacatctg aattgaggta tccagcagcc
    13861 gttctcccca gcgtgacagc ccgctaatca gtcccagaat caagcccagc gatccgccaa
    13921 ttgaaaagcc aatcagcgcc cgccaggaac tgatcgccag atgctgccac aattcgccgc
    13981 tggcggagag cgtccagaac gccgtcacca ctccttccgg tgaaggcaaa atacgcgtcg
    14041 acagccagcc aaccgaggag gccagttgcc acaccgccac gatgcccacc ggtaaaaacc
    14101 agggggcaac gcgcaataac cacttcttca ctggcgttgc catcatcgtg ttccttagct
    14161 ttgcgcgact ttacggggga taaaatcatt cgccaccgct tcgccttgcg gattcagcgg
    14221 ctgcggctgg ggaatttccg gaatggcgac atccagatgt gggaacagca actcgccaac
    14281 ccgatacgct tcttccagat gcgggtagcc cgaaagcaca aagctgtcga tgccaagcgc
    14341 ggcgtattcg ttgattcgcg cggcgaccgt aggaccatcg cccaccagcg ccgttccggc
    14401 accgccgcgc actaagccaa cgcccgccca taaattgggg ctgatctcca gattgtcgcg
    14461 cttgccgtta tgtagcgccg ccattcgctg ttgtcctacg gaatccgtcc gggcgaatgc
    14521 ggcctgtgct ttggcgatag tttcatcatc aagatgcgag attaaccgct cggcggcctg
    14581 ccacgcttcg tcgttagttt cacgaacaat cacatgcaga cgaataccga aacgaatttt
    14641 gcgtccatgc gcggcagctt tcgcccgcac ttgttcgatc ttctctttta ccagctctgg
    14701 cggttcgccc caggtgaggt agagatcaac ttgttctgcc gccagctcct gggcgacatc
    14761 tgacgatccg ccaaagtaaa gcggaggata cggctgttga atcggcggga agagcagttt
    14821 tgctccccgc acatgaatat gtttaccgtt gaaatcgacg ctttccccca gcaataagcg
    14881 ccccgccaga cctgggtaaa ttccgccgag gcttcgtagc gctcgctatg atcaaggaac
    14941 acgccgtcgc ctgccaactc ttgtggatcg ctgcctgtga ccagattaaa caacgcacga
    15001 ccatttgaga gacggtcaag cgtggcggcc tggcgagcgg caacggtagg tgaggttacg
    15061 ctgggacgca gggcgacaag aaacttcagc cgctgcgtca ccgggatcat cgacgccgcc
    15121 accagccacg catcttcaca cgatcgcccc gttgggatca gcacaccggt atagcccaga
    15181 cgatccgccg cttgcgcaat ttgttgcaga tagccgtgat caaccgggcg tgaaccttct
    15241 tccgttccaa gataatgccc gtcaccatgg gtcggtaaaa accagaacat attcagactc
    15301 ataattgttt tccttccagg tgagtgggct gccagatgcg ctggcgaata tcaacttttt
    15361 tcgacaccag acggttttca taaaacagat ctgccgtttg ctgctgtaag gcggcaactt
    15421 cagcgctcac cggtttgatg gtggtgggag gacgatgatc cagatacgag gcaatcaccg
    15481 gtgccggtaa gcccatcgtt tttgccagta aagcgatgct ttgctcgcgc tggctgcggg
    15541 ttaacgcatc ggcctcacta aaggttgcca gtacgccctg aataaaagcg ccgttttttt
    15601 ctgcataggg gcgagctgcc agataaaacg atccagtttg attgagatcg gtgccgtctt
    15661 tcagcacccg cacgccgccc tgtaataacg cagcggagta gtagggatcc cagatagccc
    15721 aggcgtcaac gttaccttgc tggaacgcgg cgcgggcatc agctggcgtc agataagtgg
    15781 gctggatgtc agtaaactta agtccggcct gacgcagtgc acgcagtaaa aggttgtgtg
    15841 aactggaacc tttctgaaag gcaactttgt gacctttaag atcggctacg gttttgatcg
    15901 ggctgttttc tgccaccaga atcacttcgg ctttgggctt cggtggctcg acgcccacat
    15961 atatcaaatc agccccggca gcctgggcaa agattggcgg aatatccccg gtactgccga
    16021 gatcaatact gccaacgttt aacgcttcca acatttgcgg acccgcgggg aactccaccc
    16081 aggagatttt tgattgcgga tagcgttttt ccagtaactg gtggcttttt gccaatacca
    16141 taccaatact gcctttctga tagcctatac gtaacgcttc aggcgaggat tctgcagcaa
    16201 ccgcaaaagt agagacgcta agcaatcccg ccagcgccag tttaatgatg ttacgcatgg
    16261 acattacctc gcagagacag aaggtcagga acctgaacat cgcggcggtg caatgcctgc
    16321 cagaaagttt ctagcgcggt atcaagacgg gtttgcagat ttggcgtgaa ctggggtttg
    16381 tgatggtaat caataacttg tgagtcatcg gcaaacacgc cgtgcaggag ctcctgggct
    16441 ttcatcgcac ttaaaactgg cttaagggca taatcgaccg ccagcagatg agccaccgta
    16501 ccgcccgtcg ccagcggtag caccactttg ccttgcaaag cgcgctctgg cagcaggtcg
    16561 agcagggttt tcagcgcgcc ggaataggcg gctttataca caggtgtagc gactatcagt
    16621 ccatcagcct gttgcagctg ttcggtgaag gtcttgagtg ccggactatc gaaacgagcg
    16681 taaagcagat cttccggggc gaagttttgc agattccagt gataaacctc tacatccagg
    16741 ccatttagtt tttcccgcgc atattccagc aaggagctgg agcgagaagg aaagcgagga
    16801 ctacccgcca gggtgatgac acgcatactc tctccttata accaattgtt ctttttttgt
    16861 taacattgat aacaattcgg tcagtctgtc ggagagacaa gaaaatgcca aatataaatt
    16921 ttgtgtatct ttttctgaaa ataaactaaa gaaagggctt tatatatcac gcatatttat
    16981 ttgttggata gtcattagat gttgaatcaa tgaattactc gttcagttaa tatttaaata
    17041 ttcatgaaat ctataaatta aagatttgtc acttattgga tttagtatgc ttgatttaaa
    17101 tggcggtcaa ttattgttat tattaagtct aatgtcattt aaag
//
LOCUS       DAETQN010000092        16593 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.092, whole genome shotgun sequence.
ACCESSION   DAETQN010000092 DAETQN010000000
VERSION     DAETQN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.092"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..586)
                     /gene="araH"
                     /locus_tag="K0W68_003003"
     CDS             complement(<1..586)
                     /gene="araH"
                     /locus_tag="K0W68_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBI3074508.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTT"
     gene            complement(601..2115)
                     /gene="araG"
                     /locus_tag="K0W68_003004"
     CDS             complement(601..2115)
                     /gene="araG"
                     /locus_tag="K0W68_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HBI3074509.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(2185..3174)
                     /gene="araF"
                     /locus_tag="K0W68_003005"
     CDS             complement(2185..3174)
                     /gene="araF"
                     /locus_tag="K0W68_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HBI3074510.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            3971..4474
                     /locus_tag="K0W68_003006"
     CDS             3971..4474
                     /locus_tag="K0W68_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBI3074511.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4553..4804)
                     /locus_tag="K0W68_003007"
     CDS             complement(4553..4804)
                     /locus_tag="K0W68_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBI3074512.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(4919..5005)
                     /gene="azuC"
                     /locus_tag="K0W68_003008"
     CDS             complement(4919..5005)
                     /gene="azuC"
                     /locus_tag="K0W68_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBI3074513.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            5268..5591
                     /locus_tag="K0W68_003009"
     CDS             5268..5591
                     /locus_tag="K0W68_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBI3074514.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            5762..6259
                     /gene="ftnA"
                     /locus_tag="K0W68_003010"
     CDS             5762..6259
                     /gene="ftnA"
                     /locus_tag="K0W68_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBI3074515.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(6297..6536)
                     /locus_tag="K0W68_003011"
     CDS             complement(6297..6536)
                     /locus_tag="K0W68_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBI3074516.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            6727..7938
                     /gene="tyrP"
                     /locus_tag="K0W68_003012"
     CDS             6727..7938
                     /gene="tyrP"
                     /locus_tag="K0W68_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBI3074517.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(8000..8665)
                     /gene="yecA"
                     /locus_tag="K0W68_003013"
     CDS             complement(8000..8665)
                     /gene="yecA"
                     /locus_tag="K0W68_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBI3074518.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(8861..8947)
                     /locus_tag="K0W68_003014"
     tRNA            complement(8861..8947)
                     /locus_tag="K0W68_003014"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8911..8913),aa:Leu,seq:taa)
     gene            complement(8960..9033)
                     /locus_tag="K0W68_003015"
     tRNA            complement(8960..9033)
                     /locus_tag="K0W68_003015"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8999..9001),aa:Cys,seq:gca)
     gene            complement(9088..9163)
                     /locus_tag="K0W68_003016"
     tRNA            complement(9088..9163)
                     /locus_tag="K0W68_003016"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9128..9130),aa:Gly,seq:gcc)
     gene            complement(9315..9863)
                     /gene="pgsA"
                     /locus_tag="K0W68_003017"
     CDS             complement(9315..9863)
                     /gene="pgsA"
                     /locus_tag="K0W68_003017"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBI3074519.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(9920..11752)
                     /gene="uvrC"
                     /locus_tag="K0W68_003018"
     CDS             complement(9920..11752)
                     /gene="uvrC"
                     /locus_tag="K0W68_003018"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBI3074520.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(11749..12405)
                     /gene="uvrY"
                     /locus_tag="K0W68_003019"
     CDS             complement(11749..12405)
                     /gene="uvrY"
                     /locus_tag="K0W68_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBI3074521.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            12701..12877
                     /locus_tag="K0W68_003020"
     CDS             12701..12877
                     /locus_tag="K0W68_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074522.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            12864..13088
                     /locus_tag="K0W68_003021"
     CDS             12864..13088
                     /locus_tag="K0W68_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBI3074523.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(13155..13877)
                     /gene="sdiA"
                     /locus_tag="K0W68_003022"
     CDS             complement(13155..13877)
                     /gene="sdiA"
                     /locus_tag="K0W68_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HBI3074524.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(14107..14859)
                     /gene="yecC"
                     /locus_tag="K0W68_003023"
     CDS             complement(14107..14859)
                     /gene="yecC"
                     /locus_tag="K0W68_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBI3074525.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(14856..15524)
                     /gene="tcyL"
                     /locus_tag="K0W68_003024"
     CDS             complement(14856..15524)
                     /gene="tcyL"
                     /locus_tag="K0W68_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBI3074526.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(15539..16525)
                     /gene="dcyD"
                     /locus_tag="K0W68_003025"
     CDS             complement(15539..16525)
                     /gene="dcyD"
                     /locus_tag="K0W68_003025"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBI3074527.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         4189 a         4277 c         3843 g         4284 t
ORIGIN      
        1 aggtggtttt attcagcaac aaaccaaaga taatcagaca cgcgacggtg agccagattg
       61 gcgcaggcag accgaaccag ttagcgtaac caagggcaaa gaagctttca tcttcgatac
      121 cgaccgcttt accgtctgaa atgatgtacg ccagacctcg aacaatctgc atcgttgcca
      181 gtgttgtgat cagagcattt attttcagtt tggcgataac aaagccattg accaggccac
      241 agagaatgcc aagcagcaac cccgctgcca cgccaatcca caggctttca gtcaggttga
      301 taaccaccgc cgtggtgaca cccgcacagg caattacgga ggcgacagaa aggtcaaagt
      361 caccggaagc aaggcagaac aacatgccac acgccaccat ccccgacatg gaaattgcca
      421 ggcccaaccc tttcatatta atgaaggtgg caaaatttgg gacaaaaatg gcacaggcga
      481 taaagagcac cgcaaacacc accagcatgc cgtactgatc ccagatacgc ccgaagctga
      541 atgacgactt aggtgcgcca gaacccgatg tagaaacaga agacatcata ctctccttac
      601 tcaggcaact gcctggctga ctttaggcat cgcaaggctc agtgcctgac gctcatctgc
      661 ctgctcgtgt aacaattcac cggcgatttc accttcccgc atcaccacaa tccggtcggc
      721 aacgccgagg acttcaggta agtcgctgga ggcaaacagc accgccacac cctgcgccgc
      781 cagcgcataa attacgttat atatttcgtg cttagcacca acatcaatgc tgcgcgtagg
      841 ttcatccagc aaaatgacct tcatctcttc cgataaccag cggcccagaa tggctttttg
      901 ctgatttccg cctgagagat tcatgatcag ttgctcagcg cccggcgttt tgatgttgag
      961 cgaacgaatg tgctgatcgg cattgttttc ttcccaaccg ttgttgatta cacaaccgcc
     1021 gagcacatgt ttgcgtctgg cactgatgtt gatattgtcg cgaacggagt gcacgggaat
     1081 aatgccttcc gctttgcgat cttccgggca gagcatcatg cctgcggcaa tggcgtggct
     1141 cggtttacga atatcgatcg gctgttggtc gatataaacc tgaccggcgg tgatttgcgt
     1201 cccgccaaac aagcctttca ttaattcgct acgccccgcc cctaccagac caaacagccc
     1261 aacaatttca ccactgcgaa ccgccagact tattggcgta cgcacgcctg gtgctttcac
     1321 agcatcaaga cgcaggcgct cctcgccata actacgaggt tgccagccgt agatatcgcc
     1381 aatgtcgcgc ccgaccatcg cctgcaccag cgcgtcgtgg tcaacctgct gcatatcggt
     1441 aaaggttttg acataacgtc catctttaaa gacagtgatg gcatcgctga gggcaaatat
     1501 ttcttccata cggtgagaaa cgtataagat cacccgcccc tcttttcgca gttcacgaat
     1561 aacgcggaaa agattgtcga tttcgcgggc agaaagggag ctggttggct catcaaaggc
     1621 gataatttta gcgttacgcg ccagcgcctt ggcgatttca accatctgcc actgaccaat
     1681 ggagagatat ttcagcggcg tgtccgggtc aatatccata ccaagatgtt taagttgtaa
     1741 acccgcctca taattcagca atgagcgatt cacaatgccg cctttatgcg gcagctggcc
     1801 gagatagatg ttttccgcga cggtcatttc cggcacgaga tgcagttcct ggtaaataat
     1861 cgccacaccc gcgttaagtg ctgcggtcgt gtcggaaaag gacatttcct gcccattaat
     1921 cactacagaa cccgtggttg gcgcatagtt gccgctgagg atttttaaga gagttgattt
     1981 tcctgcgcca ttttcaccca tcaacgcatg aacctgaccg gcatagcagt caaaactaat
     2041 atccgtcagc gccttaacgc cgggaaacgt tttaccgatg ccgcgaaatg agagatacgg
     2101 ggtagactgt tgcataacgt ctccgtgaat cactggtcgt tcacaccccc tctgcatcat
     2161 gcagagggga atttttccgg caaattactt accgcctaaa ccttttttct ccagttcttc
     2221 tttaaagttg tcacgcgtga tcagcaccac gtcggtaact tcggtaaatt ttggcggttc
     2281 aacgtctttt gctacccagt tgtaaagcat ttcgctggat ttatagccat gtacgtccgg
     2341 gcttggcagc agggaaccgt agaagccggt tgcctgtgct ttagacagtt cgctcaacgc
     2401 atccacaccg ttaatgccaa tgccgatgat atcggcagct ttaaagccct gaccttccgt
     2461 cgcgcgtacg ccgcccagca cggtgctgtc gttcataccg acgatcagcc aatgtttaac
     2521 ttccggatgt tgaaccagca ttgagttggc agcgtcaaat gcccccggga tgtcgttaga
     2581 tttggtaggt acctgataaa tttgtttttc cgggaatccg gccgctttca gcgcatccat
     2641 agaacccgtt gtacggcgac gtgccgtatc cagttcgttg gcggtaatcg ccatcaccgc
     2701 gctttctttg acatcccagc cacgtttctg catctcttta tacagttcct gaccctgacg
     2761 ttcgccaatt ttagtcgccg ccatcatcac cagcggaacg gtatccattg gcttaccttt
     2821 ggcgttaaca aactggtcat ccacggcaat gactttcata tcgtagccac gcgctttcgc
     2881 gacgatggca gagccgagtt tggggtccgg agtacaaata acgaaacctt ttgcgccact
     2941 ggcagccagg ctgtcgatcg cgttcaatgt tttttcgcca tccggcacgg caatcttaat
     3001 aacctcaaac cctaaatcct tcccggcttt atcggcaaac ttccattcgg tctggaacca
     3061 cggctcttcc ggttgcttca ccagaaaacc gagcttcagg ttctccgcca tagcggattg
     3121 tgacataacg gctgccagac caatggctgc cagggcttta gtaaatttgt gcatggttct
     3181 ctccagcttt agtgtcgttt tgtgtagggc aaaaacgaat gactttcgtt aaattaatcg
     3241 gaaaacaaag cattaccttt tatctaaaag ataagtgacc gtgttgacat agttttagcg
     3301 agaaattaat tctccatagg agagcaatat cacatcgcag aattacagtg agaacgtgca
     3361 taaatttagc gggaaaagac ataagggaaa gccaatttgt cagacaaatt gtcgaatgca
     3421 cagcagatta atccataaga ttagcctgga aatccttgtt gtctttggta cccatgcggg
     3481 atgtcttctt tttaaccagt caataggccg cattacctgg cgttgagttt ttgaaatggt
     3541 gtaataaccg caactcaaag atgtggaaaa tgcacgtcat tcatttcgcc attaattatc
     3601 actgtgctca ttaattaaca gaacacgtat aatgagagcc atctcgcaaa aatgaaaaaa
     3661 cgttttataa aatcatcact tcatcatgaa ttcaaattca ttgattaata tcaacaagat
     3721 acaaaaagca ctatcattaa aattcattgc agttacattg atttcatcaa tgaaatgtaa
     3781 aaatatataa acttgatgat ttaagcattt tcttataccc gttcagacgt tattcttatt
     3841 tcagatcatc gtcagaattg actccacgat cacatttcgg accggcagaa aggaattatt
     3901 ctgcaaacag taattatggt gttttgattt atcttgcacc tctccacttc tggatataag
     3961 gatattaggt atggcaaccg ctggaatgct tctcaaactc aactctcaaa tgaaccgcga
     4021 gttttacgca tccaatctct accttcacct gagtaactgg tgttctgaac agagtctgaa
     4081 cggcaccgcc actttccttc gcgcccaggc acagagtaat gtgacccaaa tgatgcgcat
     4141 gtttaacttt atgaagagtg tcggcgctac ccccatcgtt aaagccattg atgttcccgg
     4201 tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg atggaagaat acgagcaacg
     4261 ttctagtacg ttggcacagt tagccgatga agcgaaagaa ctgaatgatg attcaaccgt
     4321 caatttcctg cgcgatctgg aaaaagaaca gcagcatgat ggtctgttgc tgcaaaccat
     4381 tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc cctgtgcaga ccgaccaaca
     4441 tgttctgaat gtcgtgtcac accagctgca ttgatcatca tcggcgctaa tgcattgcgc
     4501 cgatgaaggt tttgagaaac cgctgcctca tctgtttgaa gcagcggttt ttttaatggg
     4561 attcaccctg tggggtaaac ttgagttcaa taagcgcgat ggctttttgg attgcccgca
     4621 tggtgaccgg gtctgcggcg gcgggatggt tagtaaagtc gatattcttc agctgactgg
     4681 acattttttc acgaacttca acgggcgcga ttacatcgag aacatccaga atttgtttga
     4741 taaccaactg gcaagcaacc acatcagaaa ccagttcctg atcggcattc agcggctggg
     4801 acatcgtaaa ctcctgatag cattttgaaa gccgttatag tagcggcttc acatcttcag
     4861 cgatagtcac atccaccgtc atcaggacac aaaaaaacct gccggagcag gttttttgtt
     4921 atcggaacat attgcctggc ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac
     4981 ttttcaggat tttgcgcagt ttcatcgcgg cactccgacc atttgttata caggtgttat
     5041 tgtctttgcg cttataatat gacaaccatc acaaaaatca atctttatgt gatacaaatc
     5101 acataaatac ccctttaatg ttataaaaat gataatcaaa aaacagcccc cctatttctg
     5161 acacctacag atggcaagaa atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc
     5221 gctgtaacgc gtaaaatagt gctttctctt actcttctgg ctggaccatg agacttctga
     5281 ttctgactct ttcattaata acgctggcgg ggtgtacggt tactcgccaa gcccatgtga
     5341 gcgaagttga cgcggcaaca ggtattgtac ggttggttta tgaccaggct tttttgcagc
     5401 atgcccatac cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag
     5461 gctataccca cgcggtcccc tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt
     5521 ccttatgttt gaatacggaa ttcactttgt catatcagtg ccatcattcc gccttccctg
     5581 tctttctcta aatattttaa tcagcgaggg gatcttcgct gattaaagaa atagcggtaa
     5641 tgcgttttat tcccattcgt atttttaata attgaagttt atattttacc ttttgcaaat
     5701 aataaaataa caaattatag tgacgccaca gcaacaaata taacctttgt ggagcactat
     5761 catgctgaaa ccagaaatga ttgaaaaact taatgagcag atgaacctgg aactgtactc
     5821 ttcactgctt tatcagcaaa tgagcgcctg gtgcagctat cataccttcg aaggtgctgc
     5881 cgcgttcctg cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc tgtttgatta
     5941 cctgactgat accggcaatt taccgcgtat taataccgtt gaatctccgt ttgctgaata
     6001 ttcctcactt gatgaattat tccaggaaac ctataaacac gaacaattaa tcacccagaa
     6061 aattaacgaa ctggctcatg ctgcaatgac caatcaggac tacccaacat ttaatttcct
     6121 gcagtggtat gtttctgagc agcatgaaga agagaaactg ttcaaatcga ttattgataa
     6181 attaagcctg gcaggcaaaa gcggcgaagg tctgtatttt atcgacaaag aactctctac
     6241 cctcgacaca caaaactaat gctctccggc ggcaggttta ctgccgccgt ttcaaatcag
     6301 tgacggcaaa tcttactctg atcggtcgaa aaaccgtctt tcgaaggtat aaatttgcct
     6361 tttgctgcca gaaacgctac cagctctccc gctgtcatcc cttctgccga gcaggtgtga
     6421 aaacgtgcct gttcaccaaa acgcgcttta atcgcagctt ccagactggc atgcgtatat
     6481 tgctcgcctg attcaatcat catatttaac acttcatgac cgtgaataga gtccatcgtt
     6541 cctcctcaaa aaaagcctag cgtagcgatt gccgcttatg aagactttgc gccagcgcag
     6601 gactgaatgc tttttattgt acatttatat ttacactata tgtaacgtcg gtttgacgaa
     6661 gcagccgtta tgccttaacc tgcgccgcag atatcactca taaagatcgt caggacagaa
     6721 gaaagcgtga aaaacagaac cctgggaagt gtttttatcg tggcgggaac cacaattggc
     6781 gcaggcatgc tggcaatgcc gctggctgcg gccggtgttg gttttagcgt tacgttaatc
     6841 ttgttgattg ggctttgggc gttgatgtgc tacacggcgc tattactgct ggaggtgtac
     6901 cagcatgttc cggcagatac cggtctgggc acgctggcaa aacgctatct gggacgctac
     6961 ggtcaatggc tgacgggctt cagtatgatg ttcttaatgt atgctctgac tgcggcatac
     7021 atcagcggtg tcggtgaatt gttggcctcc agcatcagcg actggacagg tatttcaatg
     7081 tcggcaaccg ctggcgtgct gttgttcact tttgttgccg gtggcgtggt ttgtgttgga
     7141 acatcgctgg tcgatttatt taaccgtttt ctgttcagcg ccaaaattat ttttctggtg
     7201 gtgatgctgg tattgctgct accgcatatt cacaaagtga atcttttaac cctgccgttg
     7261 caacaggggc tggctctgtc agcaatcccg gtgattttta cctcatttgg ttttcacggt
     7321 agcgtgccga gtattgtcag ctatatggat ggcaacgttc gtaagctacg ctgggtgttt
     7381 ataaccggta gtgcgatccc cctggtggca tatattttct ggcaggtggc aacgcttggc
     7441 agcattgatt caacaacctt tatgggattg ctggctaatc atgctggatt aaacgggctg
     7501 ttacaggcgt tacgcgaaat ggtggcctct ccgcatgttg agctggcagt gcatttattt
     7561 gctgatttag ccctcgccac gtcatttctc ggcgttgcgt taggcttatt tgattatctg
     7621 gctgatttat ttcagcgttc aaataccgtt ggtggacggt tgcaaactgg tgcaattacc
     7681 tttctgccgc cgttggcgtt tgcactgttt tatccacgag gatttgtgat ggcgctgggt
     7741 tacgccggtg tggcgctggc ggtactggca ttgattatcc cttcgctgtt gacctggcaa
     7801 agcagaaagc acaatcctca ggcgggttac cgggtcaaag gtggtcgtcc ggcgctggtg
     7861 gtggtgtttc tctgtggtat tgctgtgatt ggcgtgcaat ttttgattgc ggcagggttg
     7921 ttaccagaag tggggtgatc agatagcctc aaattcctta ttgggtgcca gaattaacgc
     7981 tgacacccaa tttggcctct taatgcaggc agcactgctt aaatttctta ccactaccgc
     8041 acgggcaagg atcgttacgc cccggtttct cttctgcttt gatcggttgc tgaacagctt
     8101 tttcctgcgg atgcgccatc cagtacgcat gtagatcaag cgccgccagt cgaatggcat
     8161 ctacgctctc ttcaaacgct tctggcgaca tcttttctac ccgctcgaag ttttcctcag
     8221 taccgtgcag cgcaatcgcc tccagcgctg gttttaacga atcgggcaac gttgaccagt
     8281 cagaaagtgc cacgccccgc atatagccaa agcaccactc ctcaacaatc gtcagctcgc
     8341 tgccatcaac ttctcgcaag ccgaataacg gctcaaactg ctccgggaat tcgttcagac
     8401 gctctgcggt atcggccata tgttgaaaag ccaggttcat aaagcgcgtc atctctttct
     8461 ctgacgccca gcgcggcaca tagtcagccc caccccacac ggcaaccagc cactgttccg
     8521 gttcaatctc ttgcggagaa ctcaacaccg ccgtcaataa accgtccagc tccgccacat
     8581 caaggatggc gtggttagtg ttgtatttgg tcagaatatc gtccagccat tccaactcac
     8641 tttcgtttaa cggtcccgtt ttcatacgct tttccttgtg gatctcaact cgccagcacc
     8701 tatcttacat gccggtccgt atcagagata ctttttgagt ggctttgccg gtgattaaaa
     8761 attaaggagg gtgtaacgac aagttgcagg cacaaaaaaa ccacccgaag gtggtttcac
     8821 gacactgctt attgctttga ttttattctt atctttccca tggtacccgg agcgggactt
     8881 gaacccgcac agcgcgaacg ccgagggatt ttaaatccct tgtgtctacc gattccacca
     8941 tccgggctcg ggaagaaagt ggaggcgcgt tccggagtcg aaccggacta gacggatttg
     9001 caatccgcta cataaccgct ttgttaacgc gccaaattct tcaggccttt cagccagaca
     9061 tccgcttgac gccgatgtct tttaaactgg agcgggaaac gagactcgaa ctcgcgaccc
     9121 cgaccttggc aaggtcgtgc tctaccaact gagctattcc cgcattcatc aagcaatcag
     9181 ttaatcactt gattttatta tcgtctggca atcagtgccg ccgttcgatg cgttgcattc
     9241 tacttacctg gcgcgatgag tcaacgatat ttttcaccac ttttgatcgt ttgctgaaaa
     9301 ttacgccgaa acgatcactg atcaagcaaa tctgcacgcg cagcgctcaa atattgcaac
     9361 attgaccaca gagtcagtac cgcagccaca aagaaaagtg caataccggc gtactcaacc
     9421 caaatgttcg gacgccacag cagccatgcc aacgccacca tctgggcagt ggttttcact
     9481 ttcccaatcc aggagacagc cacgctactg cgtttaccca actccgccat ccattcgcgt
     9541 agcgcagaaa taataatttc acgggcgatc atcgttgctg ccggtaaggt cacccaccag
     9601 ctgtggtaat gctcggttac cagcaccatg gcgatagcca cgagaacttt atctgctaca
     9661 gggtcaagga aagcaccaaa ccgggtactc tggttccagc ggcgtgccag aaaaccatcg
     9721 aaccagtcag tcaccgccgc gacgcagaaa atgagcgcgg cggcaaacgg cgaccaggtg
     9781 acaggcagat aaaagaccaa tacaaagaat gggataagga tgacacggaa cagtgtaagc
     9841 aacgtaggga tattaaattg cataatgacg ggtaactatc tgttgtcagt aagattaccc
     9901 ctatgttgct acagagacat caatgtttca acgaccagaa gatcttttct gccagacctt
     9961 gcgaaatacc cggcactttt gcaatttcct cgacgctggc gttacgtaaa ccttgcaaac
    10021 cgcccatata tttcaacaac atttgccgac gttttggccc gacgccttca atggtttcca
    10081 gggaactggt atttttgacc ttcgcccgtt ttttacggtg cccgccaatc gcgtgatcat
    10141 gtgattcatc gcgaatatgc tggataacat gcagcgcggg agaatctggc ggcaaactga
    10201 atccctcacc ttccggctca aagaacagcg tttccagccc agccttacga tctgctcctt
    10261 tggcaacgcc aagtagcagc ggatgatttt tatcccatga gacatccagt tcggcgaaga
    10321 catttttcgc ctgcgcaagc tggcctttgc cgccgtcgat aaggatcaca tccgggatct
    10381 tactgtcgtc aatggcttta ccataacgcc gacgcagcac ctgattcatc gccgcataat
    10441 catcgcccgg cgtgatgcct gtaatgttat agcgccgata ctccgcacgc agcgggccgt
    10501 tagcatcaaa caccacacag gaagcgacgg tttgttcgcc catggtatgg ctgatgtcaa
    10561 agcactccat ccgcttcact tccggcaatt tcaacacgct ggcaagcgcg gtcagtcgct
    10621 ggtgaacggt agattgctgc gaaagtttgc tggttaaggc cgtcgccgca ttggtgcgcg
    10681 cgagtttcag ataacgcgcc ctatcgccgc gaggtttggt ttgaacatta atcttgcgtc
    10741 ccgccagttc tgaaagggaa tcggcgagca gcgttttatc gctaagatta aaatcgagca
    10801 ggatctcacc cggtaaggtg cgcatctggc tgccttgtaa atagaactgg cctacgaagg
    10861 tttctaccac ctcgctcagt tccgtaccgc caggcacttt cgggaaatag ctgcggctgc
    10921 cgagcacttt gccctgacga atgaacaata cgtggacaca agccatgccc gcatcgaacg
    10981 ccacaccaat aacgtcgagg tcgtcgccgg tattggaaac gaattgtttt tcggtgacgc
    11041 gtcgcaccgc ctgaatttgg tcgcgaatac gtgcagcttc ttcaaactcc agattctggc
    11101 tggcagtttc catacgacta atgagttgcg taagcacctg atcatctttg ccagacaaaa
    11161 acaggcgcac atactcaacc tgctgagcgt attcttcttc actcaccagt ccttcaacgc
    11221 acggtcccag acagcgccct atctgatatt gcagacacgg acgcgagcga ttgcgataaa
    11281 cactattttc gcactggcga atggggaaaa tcttttgcag tagcgccagt gtttcacgta
    11341 cggcatagcc attcgggaac gggccgaaat attcaccttt ggcatgcttc gcaccacgat
    11401 gcatcgccag acgcgggtgg gtatcgccac tcaggaagat aaaaggataa gatttatcat
    11461 cgcgtagcaa aacgttgtaa cgcggctgat agagtttgat gtagttgtgt tccagcaaca
    11521 gcgcttcggt ttctgtatgc gtaaccgtta catcaatttg ctggatctgg gcgaccagcg
    11581 cttcggtttt gcgcgaagcg aggttgctgc ggaaatagct ggaaagccgt tttttcaggt
    11641 ctttcgcttt gccgacatag ataaccgtac caccagcatc gtacatgcga taaacgcctg
    11701 gctggcaggt tacggttttt aaaaacgctt ttgcgtcaaa ctgatcactc actgacttga
    11761 taatgtctcc gcattacaca gaccatggcg aattgccagg tgagtcagct caacatcgcc
    11821 atgaatgttt agtttactga acatacgata gcggtagctg ttcaccgttt tcggactgag
    11881 attgagctgt tctgagatct cattgacctt ctggcccttg gtgatcatca gcataatctg
    11941 caattcacgt tcagacaaac tggcaaatgg gctttctgtt ttttctggtt cgatctggct
    12001 taacgccatt tgttgagcga tatcagaagc aatgtaacgc tgccctgaat agacagaacg
    12061 aatcgcactc acgacttcct gcggagccgc gcctttgctg aggtagcccg cagcaccggc
    12121 ctgcatgact ttcgctggta aagggttttc tgtatggacg gtaagcatga tgattttgac
    12181 atcagctgtg gaacgcgcga ttttacgcgt cgcctcaaga ccgccaatgc ccggcatact
    12241 catgtccatt agcaccacgt caacggcatt tgtccggcac cacttaacgg cgtcttcacc
    12301 gcacgatgcc tcaccgacga cttttatacc ctttatatct tccagaatgc gtcgtatccc
    12361 tgcgcgcacc agttcgtggt catcaacaag tagaacgttg atcaaaggaa tatctccaga
    12421 aatagggata acgctactga tagttagtca ttcgtatatt agcggttttt attgcaactt
    12481 tgaaacgtta aaaatggtgg gtattcgatt tttctctcgt ttttgtaaat tcgattgtcc
    12541 acagttatgg tcacgcccgt caccaacggc ttccgggatc gtttcacagt aagcccggcg
    12601 atgaaatgtg acaaaaattg actttattca gcaaaaatga aaatcagccc gctgaatatg
    12661 taacattaat taacccgaac aatacgggca aaaaacattc atgataaaaa tatttattgg
    12721 tcattatatt aacgcattta atagcattgt tgacgtcacg ctcaaaaaac aaccactgct
    12781 atttttagca aagcttatgg tatactccgc cgccttaaca tttttcaccg caaattttca
    12841 ttgcaacatg acgaggaaaa taaatgagta cgcctgattt ttctactgcc gagaataatc
    12901 aagaactggc aaatgaagtc tcctgcctga aagcgatgct gacgctgatg ctacaggcga
    12961 tgggacaagc tgacgcgggc cgcgtgatgt taaagatgga aaaacagctt gcgctgatcg
    13021 aagacgaaac ccaggctgca gtattttcca aaacggttaa gcaaattaaa caagcctacc
    13081 gtcagtaata taaaaccggc tgagagcgtg cctttcagcc gatttttgca tctggcacgc
    13141 agaaagaaaa gcgatcaaat taagccagta gcggccgcgt aacaggccac ctgggtctta
    13201 tttggtgcat tgattttttt ctgcatgttt ttctgatgga aattgaccgt attctcagag
    13261 attgacaaaa tcatcgctat ctctgctgat gtcttccctt ccgccgtcca tttcagaatt
    13321 tctttttcgc gcttgctgaa attcatctct ggcgtcatca ctatttcatc atttaaacgc
    13381 atcagagcca tcagactttc gcgcaccagt aactgcattt ttaattgcag ttcatcacta
    13441 agaatgggta tttcgcgcgt gctgcaacgg gaaaaggaca aaaagcccag cgcccgattt
    13501 ggcagcatta aatactgagt gacaccgcgg cgtaaaccat gtgcgtgcgc ggcttcccat
    13561 aatggctgtg cttcgctgaa taagtcatca ttccacatta aatggccctg actaaagttt
    13621 tcagggttca gcacaggatc aattgcgaga aaattttttg cctgataata actaacccac
    13681 gactcagggt aattggtgta gaaagccact ttaggtcgcg tgaatggtac cgggtggcga
    13741 acacataacg aatagtaatc gtactccagc tgctgagcct gaagctctat ttcatggtag
    13801 acctcttctg cggcctccat cctctgaaaa cgcaacagca tcgtgcgacg ccagctgaaa
    13861 aaatccgtat cctgcatagt aaaccgcaac gcccctgaga gtgagtatca tttataatga
    13921 taattcaaac tatcacataa ataagattta tatataattt atattattca ggcaatgaat
    13981 tacttttgca agccatcgca ttctcttatg ttattaatga gttatgctga tttgttaagc
    14041 agttttatca cgcttgaaat ggcgtcccgc cccgacaggt gaatcgtcgg ggctgatttt
    14101 ttcttattat tgcagtagaa acttctcgag gaactggcgg gtgcgaggct gctggggatc
    14161 agcaaataac gcttttgctg ccccctgctc gactatccgc ccctggtcca taaaaatcgc
    14221 ccggtccgca acatcccggg caaagctcat ttcgtgcgtc acaatcacca tcgtgcgctt
    14281 ttcctgcgcc agctggcgga ttgtgttcag gacttcgccc accagctctg gatccagcgc
    14341 tgacgttggc tcgtcaaaca aaatcacctc aggacgcatt gccagcgcac gcgcaatcgc
    14401 tacacgctgc tgttgaccgc cagacaaacg acgtggatag ctggtttctt taccggccag
    14461 cccaactttc gccagcagct cgcgagcgcg cgccgtggcc tcttctttcg gttcgccttt
    14521 gacgatcacc ggcccttcaa taatgttttc cagcaccgta cgatgcggaa acaaattaaa
    14581 gttctggaag acaaacccga cgtgctgacg caactggcgg atcagcgatt tttgctgact
    14641 taatgaacgt gcagtatcaa tagtgatatc gccaacggtg atcgttcccg cttccggttg
    14701 ttccagcaga tttatgctgc gtagcaacgt ggttttgccg gaaccactcg gaccgataat
    14761 tgccaccact tcgccaggct ttacctcgag gtcgataccg tgcagcaccg tctgaccgtg
    14821 aaattttttc accaggttct taacttcaat ggcactcatt ttggttctct ctcctggcga
    14881 ttaagttgat tctcaaaatg gttctgcaac gtcgataata ctgtcgccat gatccagtag
    14941 atgagcgaag ccgccaggta catggtgaac acctccagcg tgcgcgaggt aatcaactgc
    15001 gcctgacgga acagctccgg cacctggatt gtcgcggcca gcgacgtatc ctttaccagg
    15061 ctgataaagc tgttcgacag cggcggcaac gccacccgcg ccgcctgtgg caaaatcgcg
    15121 cgacgcatag tctgccatgg cgtcatacca atactggcag ctgcttccca ctgaccttta
    15181 tcaatagaag aaatggcggc gcgcagtgtt tcggcggcat aggcggcagt gttcagcgac
    15241 agaccaatca ttgctgacgg aatgggatct aattcgatac caaactgcgg caggccgtaa
    15301 tagatcataa acagttgtgc gatgagcggt gtaccacgaa aaatagagat ataaaaacgc
    15361 gccagccagc gcacgggcca gattggcgac aggcgcatca gcgcgagaat aaacccgagc
    15421 agtaagccaa aaaacatgcc gccgatactg agttgcagcg tatacccagc ccctttcagc
    15481 aggaacggca aggaatcaat aaccagttgt atactttctt gcatgagcgt tcccggagct
    15541 aaacgtgggg atgataggcg aacagcgcag gcgcaccgcc ggtatgaata aacagaatcg
    15601 gcccttcatc tttgaagcgt ttctgactga tacagccaat cagccccgcc atcgcttttc
    15661 cggtatacac aggatcaagc agaatgcctt ccagccgcgc cagcaatttc actgcttcca
    15721 tgccttcgtc gttcggcacg ccgtagccag gtgcaaaata gtcatcccag agtataattt
    15781 ccgctgatgc ggtcagctcc agttctttcg caatcgcctg ttgtaggtta accactttcg
    15841 gcaactgatc ggcaacggaa cgcgacacgg tcacgccaat cagttcgctt tcaggcatca
    15901 ggtgttccag cccaacagcc agtccggcgt gagttccggc actgcccgat gcgactacca
    15961 ccgacgaaat attaaccgcc ccttcacact gttgcgcgat ttccagcgca ctctccacat
    16021 aacctagcgc gccaagagca ttagaaccgc caaccggaat gacatacggg cgaaagcctt
    16081 gtgcttcgac tcgcgtcgcc agctcttcca gttgggcatt gggatcggtc agtgcttcgc
    16141 acatttcaat ctgggtattg aacagatcca gcaacaagcg attgccgttg gttaaatagt
    16201 tttctgcggt tgtgccaata ggattttcca gcagcgccac acagtgcaga ccgagtttcg
    16261 ccgcaactgc ggcagtctgc cgcacatggt tagactggat caccccggca gtaatcagcg
    16321 tatcggcacc ttcgcgcaga gcatctgccg cgagaaattc tagcttacgt aatttattgc
    16381 cgcccattgc catgggggtg acgtcatccc gtttgatgaa aatttcccgt cctagataat
    16441 cagaaaagcg cggcagatat tcgagcggcg ttggcgcgcc gataaactcc aaccgtggaa
    16501 aacgggttaa attatgcagt ggcataacag cctccgatgt atgttgttgt gattttctta
    16561 ttatgcacgc tgaaaacgcg taaataaaaa agg
//
LOCUS       DAETQN010000093        16555 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.093, whole genome shotgun sequence.
ACCESSION   DAETQN010000093 DAETQN010000000
VERSION     DAETQN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.093"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="K0W68_003026"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="K0W68_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HBI3074528.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="K0W68_003027"
     CDS             433..621
                     /locus_tag="K0W68_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBI3074529.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="K0W68_003028"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="K0W68_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HBI3074530.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="K0W68_003029"
     CDS             816..989
                     /locus_tag="K0W68_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HBI3074531.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="K0W68_003030"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="K0W68_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="K0W68_003031"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="K0W68_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HBI3074532.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="K0W68_003032"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="K0W68_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBI3074533.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="K0W68_003033"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="K0W68_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBI3074534.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="K0W68_003034"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="K0W68_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBI3074535.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="K0W68_003035"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="K0W68_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="K0W68_003036"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="K0W68_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBI3074536.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="K0W68_003037"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="K0W68_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBI3074537.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12531..13677
                     /locus_tag="K0W68_003038"
                     /pseudo
     CDS             12531..13677
                     /locus_tag="K0W68_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13971..15212
                     /gene="agp"
                     /locus_tag="K0W68_003039"
     CDS             13971..15212
                     /gene="agp"
                     /locus_tag="K0W68_003039"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBI3074538.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(15250..15477)
                     /locus_tag="K0W68_003040"
     CDS             complement(15250..15477)
                     /locus_tag="K0W68_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HBI3074539.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(15498..16103)
                     /gene="wrbA"
                     /locus_tag="K0W68_003041"
     CDS             complement(15498..16103)
                     /gene="wrbA"
                     /locus_tag="K0W68_003041"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBI3074540.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            16155..>16555
                     /locus_tag="K0W68_003042"
     CDS             16155..>16555
                     /locus_tag="K0W68_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3074541.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVK"
BASE COUNT         4159 a         4071 c         4226 g         4099 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt aaattttata gacgctatat atgggtagta
    12541 atatacatgg aattagttgc actgcaaata attatttgaa acaggcctgg aacgatataa
    12601 aaaatgagta cgaaaaaaat caaacatatt caatcacgct ttttgaaaac acactggtgt
    12661 gttttatgcg gttatacaat gaactcagac gtaaagtaaa tgaagaggat actccatgtc
    12721 tggaatgtga atcactagaa aaagaatttg aggaaatgca gaatgataat gatctatcat
    12781 tatttatgag aacattgcgt actaatgata cacaaattta ttcaggggtt tcaggaggta
    12841 ttacatatac tatacaatat gttcaaaatg ttgatattgt tagagtgtcc ttgccgggca
    12901 gaggttcaga gtctatcaca gattttaaag gttattattg gtatggcttt atggaatata
    12961 ttgaaaacat tactgcgtgt gatgacattt tttctgagta ttgtttagat gatgaaaata
    13021 tgagtatcca gccagaacag ataaatacgc cgggaatatc tgatttagat actggtttta
    13081 aaagaacatg agtattcatg ggcgctgggc ggccataaga cgcaaaagca agtatacgaa
    13141 gagtttatta aaattgttga taaacaaacg atcgaggctc ttgttgatac ggcattctat
    13201 aatctcaggg aagatgtaaa gacgttattt ggcgttaatc tacaatctga caacaaaatt
    13261 caggggcagg gtggtttttt atcatggagt tttttgtctt ttaaaaaaga atttattgat
    13321 agttgcttga atgacaaaaa atgcatactg catttacccg aatttatatt taatgataac
    13381 aaggcacgcc ttgttttaga caccgaccca gagcagaagg ttaatgaggt gaaaaatttc
    13441 ctcactgctc tttcagatag catttgctca ttatttattg tttacagcaa tgtggcatca
    13501 atctccttag ggaatgaatc cttttcaaca gatgacgacc ttgagtatgg gtatttaata
    13561 aacactggca atcattatga cgtttacctc cctcctgaac tttttgctca ggcttacgag
    13621 ttaaacaata aggaaaggaa tgcgcaaatt gattttttaa ctcgttatgc aatttaatga
    13681 caaaggcatg tgctaaaaac cattgttatt agcctcactt ctcttattat tggtactatt
    13741 gcctctgtat tagtaacgcc gctgatattc tgtttatcca caggtgcaat tatcagcggc
    13801 gtacgcgagg caggggctaa tcaggcatag tttgcgtcaa accttgcctg tttttgaaga
    13861 tgtatataga aaaacaggcg ttcaacaagc cattttgcga acctgttccc ggaaaaaagt
    13921 catatttctg tcacactctt taatgattga taacaaaaga ggtgccagga atgaacaaaa
    13981 cgctaatcgc cgcaactgtg gcagggatag ttttactcgc ttcaaatgct caggcacaaa
    14041 ccgtaccgga aggctatcag ctacagcaag tgctcatgat gagccgccat aacttacgtg
    14101 cgccgctggc gaacaatggc agtgtgctgg agcagtcgac gccgaataaa tggccagaat
    14161 gggacgtccc cggtgggcaa ctcaccacca aaggtggcgt gctcgaagtg tatatgggcc
    14221 attacatgcg tgaatggctg gcagagcagg ggatggtgaa atcgggggaa tgcccgccgc
    14281 cggacaccgt ttatgcctat gccaatagtc tgcaacgtac cgttgcgacc gcacagttct
    14341 ttattaccgg cgcattcccg gggtgtgata ttcctgtgca tcaccaggaa aaaatgggca
    14401 ctatggaccc aacctttaac ccggtgatca ccgatgattc cgccgcattc agtgaacagg
    14461 cggtggcggc aatggagaaa gagctcagca aactccagct taccgacagc taccagctac
    14521 tggaaaaaat cgttaactat aaagattccc ctgcctgtaa agagaaacaa cagtgttcgc
    14581 tggtggatgg caaaaatacc tttagcgcca agtatcaaca agaaccaggt gtttccgggc
    14641 cgctgaaagt cggcaactcg ctggtagatg cgtttacttt gcaatattac gaaggttttc
    14701 cgatggatca ggtggcctgg ggagaaatca aatctgacca gcagtggaag gtgttgtcga
    14761 agcttaaaaa cggctaccag gacagcctgt ttacctcacc ggaagtggcg cgcaatgttg
    14821 cgaaaccgct ggtcagttat atcgacaaag ctctggtcac cgatcgcacc agcgcaccga
    14881 aaattacagt gttggttggg cacgactcca acattgcctc tctgttaacg gcgctggatt
    14941 tcaaaccgta tcagttgcat gaccagaacg aacgcacgcc gattggcggc aaaatcgttt
    15001 tccagcgttg gcatgacagc aaagccaatc gcgatttgat gaaaattgaa tatgtgtatc
    15061 agagtgcgga acagttacgt aatgccgatg cgttaaccct gcaggcacct gctcagcgtg
    15121 tgacgctgga attaagcggt tgcccgatag atgccaacgg tttctgcccg atggataaat
    15181 ttgatagcgt gttgaatgaa gcggtgaaat aacagaaaac tcccccgcga gaagcggggg
    15241 agtcgctggt taaacgtttt tacgttcgat ggtctgttcg ccccaaaaaa gcgaatcttt
    15301 atcggcctta gcaaaggctt tgactaacac ttcatcgcta ccttcttccc aaatcttttc
    15361 tgccattttt tcgtcgtacc cggcgacttc gaaaatggcc tcggctattt ccggcgacgt
    15421 attgcgcaga gatgcccatt caccgacgtg atgagctttc gcttcttgag ttggcatgcg
    15481 tatcctcctg ttgaagatta gccgttaagt ttaactgcca gacctgcgac atattcccct
    15541 tgataacgag caatagacag ttcttcctgg cttggctggc gtgagccgtc accgcctgcg
    15601 atggtggttg cgccgtacgg cgtaccgccg cgaacctgtg aaacgtcaaa taattcctgc
    15661 gctgcgtagc caatggggac aatcaccatg ccgtgatgcg caagggtcgt ccaggttgaa
    15721 gtaatagttt gttcctgacc gccgccagta ccggtggaac taaagacgct cgccagtttt
    15781 ccgtatagtg cgccggaagc ccacaggccg cccgtctggt cgaggaaggt acgcatttga
    15841 ccggacatgt tgccaaagcg ggtaggtgta ccaaaaataa tggcgtcgta atcggccagt
    15901 tcttgcgggg ttgcaaccgg tgcagtttgc gttttaccgc ctgctttttc aaataattgc
    15961 ggcggcatgg tttccggtac acgcttaacg acaacttctg cgccatcgac tttgcttgca
    16021 ccctcagcga ctgcgcgtgc catcgtttca atatgtccgt acatggaata ataaagcact
    16081 agtattcttg ccttaacact catatcattg ttctcctgtt caatgaacca cttagataag
    16141 catagtccga agatatgaag ctgctagtga catacatcag gtggagtact aaagagcaag
    16201 actcaggaga ctccttaaga cggcaaacaa acctcattga tgctttctac tctaagcaca
    16261 aaaacgatta ttaccttctt cctgctcaca gatatgttga taagggtaaa tctggttttc
    16321 accagcaaca taagaatcaa ggttctgact tcagacgtat gtttgaaaat gttatgtctg
    16381 gggctattcc agaaggttcc ttgattgttg tagagaactt tgaccgcttc agtagggctg
    16441 atatcgacac agctatagat gatgtcaggc agatactgag gaagggggtc tctatactga
    16501 ctctgggtga tggtgaactc tacgacaagt cagccttaac agaccctgtg aagtt
//
LOCUS       DAETQN010000094        16469 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.094, whole genome shotgun sequence.
ACCESSION   DAETQN010000094 DAETQN010000000
VERSION     DAETQN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.094"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="K0W68_003043"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="K0W68_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBI3074542.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="K0W68_003044"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="K0W68_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBI3074543.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="K0W68_003045"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="K0W68_003045"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBI3074544.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="K0W68_003046"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="K0W68_003046"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBI3074545.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="K0W68_003047"
     CDS             complement(5254..6294)
                     /locus_tag="K0W68_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBI3074546.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="K0W68_003048"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="K0W68_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBI3074547.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="K0W68_003049"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="K0W68_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBI3074548.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="K0W68_003050"
     CDS             7229..7669
                     /locus_tag="K0W68_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBI3074549.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="K0W68_003051"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="K0W68_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBI3074550.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="K0W68_003052"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="K0W68_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBI3074551.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..11120
                     /gene="rsxC"
                     /locus_tag="K0W68_003053"
     CDS             8898..11120
                     /gene="rsxC"
                     /locus_tag="K0W68_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBI3074552.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQTNAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
     gene            11121..12179
                     /gene="rsxD"
                     /locus_tag="K0W68_003054"
     CDS             11121..12179
                     /gene="rsxD"
                     /locus_tag="K0W68_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBI3074553.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            12183..12803
                     /gene="rsxG"
                     /locus_tag="K0W68_003055"
     CDS             12183..12803
                     /gene="rsxG"
                     /locus_tag="K0W68_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBI3074554.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            12807..13502
                     /gene="rsxE"
                     /locus_tag="K0W68_003056"
     CDS             12807..13502
                     /gene="rsxE"
                     /locus_tag="K0W68_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBI3074555.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            13502..14137
                     /gene="nth"
                     /locus_tag="K0W68_003057"
     CDS             13502..14137
                     /gene="nth"
                     /locus_tag="K0W68_003057"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBI3074556.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            14748..16250
                     /gene="dtpA"
                     /locus_tag="K0W68_003058"
     CDS             14748..16250
                     /gene="dtpA"
                     /locus_tag="K0W68_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBI3074557.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            16355..>16469
                     /locus_tag="K0W68_003059"
     CDS             16355..>16469
                     /locus_tag="K0W68_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBI3074558.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
BASE COUNT         3850 a         4205 c         4205 g         4209 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat taaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcctctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc gcgccaaagc gcgcaagctg gaacagcaac
    10621 aggctaatgc ggaaccagaa gaacaggtcg atccgcgcaa agccgccgtc gaagccgcta
    10681 ttgcccgtgc caaagcgcgc aagctggaac agcaacaggc taatgcggaa ccagaagaac
    10741 aggtcgatcc gcgcaaagcc gccgtcgaag ccgctattgc ccgtgccaaa gcgcgcaagc
    10801 tggaacagca acagactaac gcagaaccag aagaacaggt tgatccgcgc aaagccgccg
    10861 tcgaagccgc tattgcccga gccaaagcgc gcaaactgga acagcaacag gctaatgcgg
    10921 aaccagaaga acaggttgat ccgcgcaaag ccgccgtcga agccgctatt gcccgtgcca
    10981 aagcgcgcaa gctggaacag caacagacta acgcagaacc agaagaacag gttgatccgc
    11041 gcaaagcggc agttgccgcg gctattgccc gcgctcaggc caaaaaagcc gcccagcaga
    11101 aggttgtaaa cgaggactaa atggtattca gaatagctag ctccccttat acccataacc
    11161 agcgccagac atcgcgcatt atgctgttgg tgttgctcgc agccgtgcca ggaatcgcag
    11221 cgcaactgtg gttttttggt tggggtactc tcgttcagat cctgttggca tcggttagta
    11281 ctctgttagc cgaagctctc gtactcaaac tacgcaagca gtcggtagcc gcaacgttga
    11341 aagataactc agcattgctg acaggcttat tgctggcggt aagtattccc cccctcgcgc
    11401 catggtggat ggtcgtgctg ggtacggtgt ttgcggtgat catcgctaaa cagttgtatg
    11461 gcggtctggg gcaaaacccg tttaatccgg caatgattgg ttatgtggtc ttactgatct
    11521 ccttcccggt gcagatgacc agctggttac cgccacatga aattgcggtc aacatccctg
    11581 gttttatcga cgccatacag gttattttca gcgggcatac cgccagtggt ggtgatatga
    11641 acacactacg cttaggtatt gatggcatta gtcaggcgac accgctggat acatttaaaa
    11701 cctctgtccg tgccggtcat tcggttgaac agattatgca atatccgatc tacagcggta
    11761 ttctggcggg cgctggttgg caatgggtaa atctcgcctg gctggctggc ggcctgtggt
    11821 tgctatggca gaaagcgatt cgctggcata ttcccctcag cttcttagta acgctggcgt
    11881 tatgcgcaac gttgggctgg ttgttctcac cagaaacact ggcagcaccg caaattcatc
    11941 tgctgtctgg tgcgaccatg ctcggcgcat tctttatttt gactgacccg gttaccgctt
    12001 ctacgaccaa tcgtggtcgt cttattttcg gcgcgcttgc gggcttatta gtctggttga
    12061 tccgcagttt cggcggctat cctgacggcg tggcttttgc cgtcctgctg gcgaacatca
    12121 cggttcctct gatcgattac tacacgcgtc cgcgcgtcta cggccatcgc aaagggtaaa
    12181 ccatgctgaa aactatccga aaacacggca ttacgttagc gctatttgca gcgggttcaa
    12241 cagggttaac tgcggccatc aaccagatga ccaaaacgac gattgctgaa caggccagtc
    12301 tgcaacaaaa ggcattattt gatcaggtgc tgccagccga acgctataac aatacgctgg
    12361 cacagagttg ctatctggta actgcgccag agttaggtaa aggtgagcat cgggtttaca
    12421 tcgccaaaca ggatgacaaa ccggtagccg ccgttctgga agcaaccgcg ccagatggct
    12481 attccggtgc gattcagctg ctggtgggag ccgattttaa cggcacggta cttggcacgc
    12541 gcgtgacaga gcaccacgaa acgccagggc ttggcgataa aatcgaactg cgcctttctg
    12601 actggatcac ccattttgcg ggtaaaaaaa tcagtggtgc ggatgatgcg cactgggcgg
    12661 tgaagaaaga tggtggtgat ttcgaccagt tcaccggcgc gacgattact ccccgcgcgg
    12721 tggttaatgc ggtaaaacgc gccggattgt acgctcagac gttaccggca caactttctc
    12781 aacttcctgc ctgtggagaa taaagcgtga gcgaaattaa agacgttatt gttcaggggt
    12841 tgtggaaaaa caactctgcg ctggtccagt tgctcggcct ttgtcctctg ttggcggtca
    12901 cgtccactgc cactaacgct ctgggtttag gacttgcgac tacgctggta ctgacgctga
    12961 ccaacctgac catttcgacg ctgcgtcact ggacgccagc cgagatccgc attcccattt
    13021 acgtgatgat catcgcctcg gtggtcagcg ctgtacagat gctgatcaac gcctacgcct
    13081 ttggcctgta tcaatcatta gggattttta ttccgctgat tgtcactaac tgtatcgttg
    13141 tgggccgcgc tgaagccttc gccgccaaaa aaggtccggc gctttcggca ctggacggct
    13201 tttcaattgg tatgggcgca acctgcgcca tgttcgtgct gggttcacta cgcgaaatta
    13261 tcggcaatgg cacattgttt gacggtgcag atgcgctgtt aggtagctgg gcaaaagtat
    13321 tacgcgtgga gattttccac accgactccc ctttcctgct ggcgatgctg ccaccaggtg
    13381 catttattgg cctgggactg atgctggcag gaaaatacct gattgatgaa agaatgaaaa
    13441 agcgccgtac tgaagcagct gccgaacgtg cattgccaaa cggtgaaaca gggaatgtct
    13501 gatgaacaaa gcaaaacgcc tggagatcct cactcgcctg cgtgagaaca atcctcatcc
    13561 caccaccgag cttaatttca gttcgccttt tgaattgctg attgccgtac tgctttccgc
    13621 tcaggcgacc gatgtcagtg ttaataaggc gacggcgaaa ctttacccgg tggcgaatac
    13681 gcctgcagcg atgcttgaac tgggcgttga aggggttaaa acctatatca aaacgattgg
    13741 gctttataac agcaaagcag aaaatatcat caaaacctgc cgtatcttac tggagcagca
    13801 taatggcgag gttccggaag atcgtgctgc gcttgaagcc ctgcccggcg taggtcgtaa
    13861 aacagccaac gtcgtattaa acactgcatt cggctggccg actattgctg tcgacacgca
    13921 cattttccgc gtttgtaatc gtactcaatt tgcgccgggg aaaaacgtcg aacaggtaga
    13981 agaaaagcta ctgaaagtgg ttccggcaga gtttaaagtc gactgccacc attggttgat
    14041 cctgcacggg cgttatacct gcattgcccg caagccccgc tgtggctctt gtattattga
    14101 agatctttgt gaatacaaag agaaagttga catctgaaga aaaggggtaa caccgattac
    14161 cccattgata acctttcttt atcctctttt aaaacatctt tttaacgtga atgatgccat
    14221 tgcttagcgt tatcatcagg taatccgttt gcggataacc aaaaatgcag gttaattgtt
    14281 tttttaatag cgaaattttc tattcattcg tgatcaagat cacgatgctg tcgcaatgta
    14341 aaaacattgc aagaatcagg ttaaaataat ttaaatttac acacgcaaca aatattgacc
    14401 tacaaaacat tacactggct atttttcaga aactggacta tctcactagt aaaaacgcta
    14461 aatagcagaa catatcgccg ttaagccatc acccccctct cagtgcagtg aaaaaatctg
    14521 ccattacgtt ttttgaaaaa tttaacgctg gataacattt cccggaatgg ttgaattccc
    14581 cgcctcagtt atatgtaaca gattattaca aaggacttgt ctgaaagtgc aagatagtga
    14641 acattacctg ccgtttcccc tcccactata acaattgcgc gtatgttttt tagacataac
    14701 gcgagaaagc acccccgtta atatgggatg taaaaaaaga ggtaaaagtg tccactgcaa
    14761 accaaaaacc aactgaaagc gtcagtttga acgctttcaa acaaccgaag gcgttctatc
    14821 tcatcttctc gattgagtta tgggaacgtt ttggttatta cggcctacaa ggaattatgg
    14881 ctgtttacct ggttaaacaa ctgggtatgt ctgaagcgga ttcaatcacc cttttctctt
    14941 cctttagtgc cctggtttat ggtctggtcg ctatcggcgg ctggttaggt gacaaggtac
    15001 tgggtactaa acgcgtaatt atgctcggtg ctattgtgct ggcgattggt tatgctctgg
    15061 ttgcctggtc tggtcacgac gccggtatcg tttatatggg tatggcggct attgcggtcg
    15121 gtaacggcct gtttaaagct aacccatctt ctctgctttc tacatgctat gagaaaaacg
    15181 acccgcgtct ggacggtgca ttcaccatgt actacatgtc cgtcaacatc ggctctttct
    15241 tctctatgat tgctacgccg tggctggccg cgaaatacgg ctggagtgtt gcgtttgcgt
    15301 tgagcgttgt aggcctgctg atcactatcg ttaacttcgc cttctgccaa cgctgggtta
    15361 aacagtacgg ttcaaaacca gacttcgagc ctatcaacta ccgtaacctg ctgctgacca
    15421 ttattggtgt tgtggcactg atcgctatcg ccacctggct gctgcacaat caggaagttg
    15481 cgcgtatggc gctgggcgtt gttgccttcg gtatcgtggt tatcttcggt aaagaagcct
    15541 tcgcgatgaa aggtgctgcg cgtcgtaaaa tgatcgttgc cttcatcctg atgctcgaag
    15601 ccattatctt cttcgtgctg tacagccaga tgccaacgtc actgaacttc tttgcgattc
    15661 gtaacgttga gcactccatt ctgggtctgg ccgtagaacc tgagcagtat caggcactga
    15721 acccgttctg gatcatcatc ggtagtccga ttctggccgc tatctataac aagatgggcg
    15781 ataccctgcc gatgccaacc aagtttgcaa tcggcatggt gatgtgttct ggtgcgttcc
    15841 tgattctgcc gctgggtgcg aaattcgcgt ctgacgctgg tatcgtgtct gtaagctggc
    15901 tggtcgcaag ctatggcctg cagagcatcg gggaactgat gatctctggt ctgggtctgg
    15961 caatggttgc acaactcgtt ccgcagcgtc tgatgggctt cattatgggt agctggttcc
    16021 tgaccactgc cggtgcaaac ctgattggtg gttatgttgc gggtatgatg gctgtgccgg
    16081 ataacgttac cgacccgctg atgtcactgg aagtttatgg tcgcgtattc ttgcagattg
    16141 gtgtcgctac tgccattatt gcagtactga tgctgctgac cgcgcctaaa ctgcaccgta
    16201 tgacgcagga tgacgctgca gacaaagcgg cgaaagctgc tgtagcgtaa aattcaggga
    16261 aactctttta caagccgcta acttttcgtt agcggctttt ttttgttcag caacgcacta
    16321 ccatacttta aaccacagac aaaaggagtt accgatgaaa ttgttctaca aaccgggtgc
    16381 ctgctctctc gcttcccata tcaccctgcg tgagagcgga aaggatttta ccctcgtcag
    16441 tgtggattta atgaaaaaac gtctcgaaa
//
LOCUS       DAETQN010000095        16395 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.095, whole genome shotgun sequence.
ACCESSION   DAETQN010000095 DAETQN010000000
VERSION     DAETQN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.095"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..578
                     /locus_tag="K0W68_003060"
     CDS             <1..578
                     /locus_tag="K0W68_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBI3074559.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            581..1744
                     /locus_tag="K0W68_003061"
     CDS             581..1744
                     /locus_tag="K0W68_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBI3074560.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1828..3453
                     /locus_tag="K0W68_003062"
     CDS             1828..3453
                     /locus_tag="K0W68_003062"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBI3074561.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="K0W68_003063"
     CDS             complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="K0W68_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBI3074562.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="K0W68_003064"
     CDS             complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="K0W68_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBI3074563.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4505..5254
                     /gene="yjfP"
                     /locus_tag="K0W68_003065"
     CDS             4505..5254
                     /gene="yjfP"
                     /locus_tag="K0W68_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBI3074564.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="K0W68_003066"
     CDS             complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="K0W68_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBI3074565.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="K0W68_003067"
     CDS             complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="K0W68_003067"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBI3074566.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7533..8931
                     /gene="ulaA"
                     /locus_tag="K0W68_003068"
                     /pseudo
     CDS             7533..8931
                     /gene="ulaA"
                     /locus_tag="K0W68_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8947..9252
                     /gene="ulaB"
                     /locus_tag="K0W68_003069"
     CDS             8947..9252
                     /gene="ulaB"
                     /locus_tag="K0W68_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBI3074567.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9262..9726
                     /gene="ulaC"
                     /locus_tag="K0W68_003070"
     CDS             9262..9726
                     /gene="ulaC"
                     /locus_tag="K0W68_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBI3074568.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9740..10390
                     /gene="ulaD"
                     /locus_tag="K0W68_003071"
     CDS             9740..10390
                     /gene="ulaD"
                     /locus_tag="K0W68_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBI3074569.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10400..11254
                     /gene="ulaE"
                     /locus_tag="K0W68_003072"
     CDS             10400..11254
                     /gene="ulaE"
                     /locus_tag="K0W68_003072"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBI3074570.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11254..11940
                     /gene="ulaF"
                     /locus_tag="K0W68_003073"
     CDS             11254..11940
                     /gene="ulaF"
                     /locus_tag="K0W68_003073"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBI3074571.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12037..12588
                     /locus_tag="K0W68_003074"
     CDS             12037..12588
                     /locus_tag="K0W68_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HBI3074572.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12663..12978)
                     /locus_tag="K0W68_003075"
                     /pseudo
     CDS             complement(12663..12978)
                     /locus_tag="K0W68_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            13305..13700
                     /gene="rpsF"
                     /locus_tag="K0W68_003076"
     CDS             13305..13700
                     /gene="rpsF"
                     /locus_tag="K0W68_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBI3074573.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13707..14021
                     /gene="priB"
                     /locus_tag="K0W68_003077"
     CDS             13707..14021
                     /gene="priB"
                     /locus_tag="K0W68_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBI3074574.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            14026..14253
                     /gene="rpsR"
                     /locus_tag="K0W68_003078"
     CDS             14026..14253
                     /gene="rpsR"
                     /locus_tag="K0W68_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBI3074575.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14295..14744
                     /gene="rplI"
                     /locus_tag="K0W68_003079"
     CDS             14295..14744
                     /gene="rplI"
                     /locus_tag="K0W68_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBI3074576.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14856..15827)
                     /locus_tag="K0W68_003080"
     CDS             complement(14856..15827)
                     /locus_tag="K0W68_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBI3074577.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15880..16296)
                     /locus_tag="K0W68_003081"
                     /pseudo
     CDS             complement(15880..16296)
                     /locus_tag="K0W68_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            complement(16313..>16395)
                     /locus_tag="K0W68_003082"
     CDS             complement(16313..>16395)
                     /locus_tag="K0W68_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3074578.1"
                     /translation="KSLSFSKSVELHDKVIGYYLNIKHYQ"
BASE COUNT         3852 a         4004 c         4353 g         4186 t
ORIGIN      
        1 ttggtagacc gaataatcct tttgaaccgc gtcgcaatcg ctacgcacaa aaatacttaa
       61 cgttggcgct aatgggcggt gccgcttttt tcgtattgaa aggttgtggc gatagtagcg
      121 atgtcgataa cgacggcgac ggaacgtttt acgcgacagt gcaggattgt attgatgacg
      181 gtaataatgc cgacatttgc gcgcgtggct ggaacaacgc caaaacggca ttttatgccg
      241 atgttccgaa gaatatgact cagcagaact gtcagtctaa gtacgaaaat tcctactacg
      301 acaatgttga acagagttgg atcccggtcg tttctggatt tttgttaagc cgggttattc
      361 gtaaagatcg cgatgagccg ttcgtctata acagcggcgg atcctcattt gcttcccgcc
      421 ccgtctggcg caacacttcc ggtgattact cctggcgctc cggttctggc aaaaaagagt
      481 cttactcttc aggcggcttt accaccaaaa aagcgtcaac cgtttcgcgc ggcggctatg
      541 gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctgagac acaacgttcc
      601 tgtgcggcgg gatctggacc aaattgccgc tgacaacggt ttcgactttc atatcatcga
      661 caatgaaatc tattgggacg agagtcgggc ttaccgtttt actctgcgcc agattgaaga
      721 gcagatcgaa aaaccaactg cggaactgca tcagatgtgc cttgaggtgg tggatcgcgc
      781 ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact gggatgtgat
      841 cgccgaaagc tggcacgcca gcgatccttc gctgtatggc cggatggatt ttgcctggtg
      901 tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgactt cgttgtatga
      961 gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca ttattccgcg
     1021 tgatgccgat cagtacaatg ccattcagga acgcctgatt tcacgcttta gcgagcttta
     1081 tagccgggag ccgttttatt tctgctgctg tcaggacacc gatgaagaca ggactaccgt
     1141 gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta tctacattga
     1201 agatctcggt ttgggcgtcg gcggtgtact gaccgatctt gatgataatg tcatccagcg
     1261 tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc cgctgctgtg
     1321 caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta ataaagggct
     1381 aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat cctggtttga
     1441 gggtgaaaaa tcgcagattg ccgctggcga aagctatgtg cgtaaaccga tctactcgcg
     1501 cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc acgctgatgg
     1561 tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccgc gatttggcga
     1621 tagctacaca ctcatcgata gctggattgt cgatgatgaa gcgtgcggaa tggggatccg
     1681 tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt acattgctgg
     1741 ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtggcc atactaatgg
     1801 tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccgt ttttaatcaa
     1861 cctataccat taaataacag caacttatac ctgtctgatg gcgcgctttg cgaagcggta
     1921 acgcgtgaag gtgctggctg ggatagcgat tttctagcca gtattggtca gcagttagga
     1981 acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga actgttgcgc
     2041 tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg gcacctgctg
     2101 atgcaggtgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc
     2161 ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg
     2221 ttatgtccaa taacgatgac ctttgccgcc acgccattgt tgttacagat gttacccacg
     2281 ccgtttcagg actggaccac gccgctgctg agcgatcgct acgattctca cttattgcca
     2341 ggtgggcaaa aacgcggttt gttgattggc atgggaatga cggaaaagca gggtggttcc
     2401 gatgtcatga gcaacaccac ccgtgcagag cgtttggaag atggctctta tcggctggtg
     2461 gggcataaat ggtttttctc ggttccgcaa agcgatgcgc atctggtgct ggcgcagacc
     2521 acgggcggtc tgtcctgttt ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg
     2581 attcgcctcg aacggctgaa agataagctg ggtaatcgct ctaacgccag ttgtgaagtg
     2641 gagtttcagg atgccattgg ctggttattg gggcaggaag gggaaggaat tcgtctgatc
     2701 ctgaaaatgg gggggatgac gcgttttgat tgcgccctgg gtagccatgc catgatgcgc
     2761 cgtgcgtttt cgctggcgat ttatcatgca catcaacgcc atgtttttgg taatccattg
     2821 atccaacagc cccttatgcg tcatgtctta agtcgcatgg cacttcagct tgaagggcaa
     2881 acggcgttgc tgtttcgtct tgcgcgagcg tgggaccggc gcgccgatgc caaagaagcc
     2941 ctgtgggcgc gtttatttac gcctgcggca aaatttgtga tctgcaaacg aggtatgccg
     3001 tttgtggccg aagcgatgga ggtgctgggc ggcattggtt attgcgagga gagcgagctg
     3061 ccgcggcttt accgggagat gccggtaaac agtatttggg aaggttccgg caatattatg
     3121 tgcctggatg tgttgcgcgt tctcaataag caagcgggcg tatacgactt attgtcggaa
     3181 gcatttgtgg aagtgaaagg gcaggatcgc tattttgatc gcgcggttcg tcgtttacag
     3241 cagcagctgc gtaagccagc tgaagaactg gggcgagaga ttactcatca gctattcctg
     3301 ctgggctgcg gtgcgcaaat gttgaaatat gcttctccgc caatggcgca ggcgtggtgt
     3361 caggtgatgt tagatacgcg cggcggcgta cggttgtcag agcagatcca gaatgattta
     3421 ttgctgcggg cgacgggggg agtgtgtttg taaacgtata cgactgatgc gacgctggtt
     3481 tcgattaact caatgaaata tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc
     3541 cggcattgtt catttgctgt agatgacact catgcataca gtatcgcctg tacgcgccag
     3601 ttatcaggct gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca
     3661 tcggccttta acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact
     3721 gagatctgac ctatttcatt caggcccgtc acgcagtcag cactggcgaa ttctgcagac
     3781 tgtgccgtgg cgctcgcagg ataagtcact aattgcagcg agggtgaggc aagtaattgt
     3841 ttcatcgtca gctccatttt tcgtttcccc acatgttgca gggcattcca tcgcggtaat
     3901 aaacgcccag gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa
     3961 gcaatgtaaa gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga
     4021 ataccactgt cctgtcacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt
     4081 tgcagcgaca gcttttgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat
     4141 cgtgcttacg ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg
     4201 atggtccgtt actggcggtg ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa
     4261 catcaacacc aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac
     4321 catagtgtag tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta
     4381 cgcgattttc gaatgaaaac gtgacgaaag cgtaacccac atctcaacat tatgaactaa
     4441 taccgccagc agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag
     4501 acagatgatt gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc
     4561 tgtcgggcaa aaagataccc cgttaccgtg cgtaattttt tatcacggct ttacttcatc
     4621 cagcctggtg tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat
     4681 gccggatgcg cccgatcacg gtagccgttt tagtggcgac gaagcgcggc ggttaaatca
     4741 attctggcaa atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc
     4801 cgaagaaaac tggctgcttg atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat
     4861 gacggcactg ggaattaccg ctcgccaccc cacggtgaaa tgtaccgcca gcatgatggg
     4921 atcgggctat tttacatcgc tcgcccgttc actgtttcca ccgctgatac ctgaaacggc
     4981 agcacagcag aatgaattca ataacattat cgcgccgctg gcagagtggg aagcgacaaa
     5041 ccacctggaa caacttgggg acagacctct actgctgtgg catggcctcg acgatgacgt
     5101 tgtgcctgcc gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa
     5161 actgctaacc tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga
     5221 tgctgccgtg acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc
     5281 ttccagttgt tgcaggattt ccgggttagc atttttgccg gtgataagca tatcgatttg
     5341 atcggcacgg ctaaaaagca ttcccgcgcg ttcgccaatc ttactgctat caaccagtac
     5401 caccagtttc cctaccacgc tcagcatctt ctgctctgcc attgctgtca gcatatcggt
     5461 tttatacaac ccttctgcgg tcagcccttt tccgctggta aacatccagt gcccggcata
     5521 gagactgttt tcgctgccct gcgggcttaa agtgatggac tgacttttgt tgtactgtcc
     5581 acccataatg atcacgctgt catgttcttg atcgatcagg taatttgcca gcggtagata
     5641 attagtgatg atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga
     5701 gccgcagttg atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcaat
     5761 acgtactttt tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg
     5821 ctgttgggta atagcttctg cgccattgcg cacctttttc agtttgccgc gttcgtcaag
     5881 tttattgata tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc
     5941 aacggtcaca aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc
     6001 agtcatgagc tattccgata aaatttgatt tttttagatg atatttgaaa tagctaggaa
     6061 atactacgcc ggatagcgcg aacttctcca cgctatccgg tataaacgac agattacagg
     6121 aatgacttga acggcagatc cggttcaata gtgaagcaat catcgaaacc gcgcggatag
     6181 tggtactcga agttgtcttt atccagcggc caggtaaatt tgccgccaac ctgccagata
     6241 aacggcttga agccatactt cagacgatct tttttcatct cccacagcac gcggatctct
     6301 tgcggatcgg cctggaagtt tgaccagatg tcgtggtgga acgggataac cactttcgca
     6361 ttcagggctt cacccatacg cagcatatcg gcgctggtca ttttgtcggt aataccgcgc
     6421 gggttctcac catacgatcc taacgccaca tcgatctgat gttcgttacc gtgcttcgca
     6481 taatagttag agtagtggga atcgccactg tgatataggg aaccgccagg ggttttgaac
     6541 aggtagttta ctgcgcgatc gtccatgcca tctggcagta cgccagccgc tttttgatcg
     6601 gcaggcaggg tgatcagtgc agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct
     6661 ttcactttta caacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag
     6721 ccaatccaca aatcaacgca ggttttcggt ccaatgaatg gtacgtcatc agcacagttc
     6781 tgcatcacgg cagcagcgac gttaacgtcg atatgatcgt tgtgatcgtg agtcgccagt
     6841 accgcgtcga tctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgtaggttt
     6901 ggctgcagtt ttttcacgcc agccatgcgc tgcatctggt gaccctgttt cattaacggg
     6961 ttaccgtgac tttgtttgcc agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct
     7021 tctgatttca accagatccc ggtgcagcct agccaccaca tcgcaaatgt gccaggggcg
     7081 acctgctctt gttcaatttc ttcattcagc cagctacccc actccgggaa agtgctcagg
     7141 atccaggatt ctcgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt
     7201 tcaattcgaa tcaatatgtg attggttttg attaatcctg acactatttt ttcaggaagg
     7261 caatgaccat tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga
     7321 tttaatggaa tgtgtagtta aattaactaa atgaatttaa ataaataatt gtttcgttgt
     7381 gtgaatccca ctctatccgt gtggaattat ttgcgggtcg cgtcacattt aatcataaat
     7441 aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga
     7501 atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg
     7561 tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg
     7621 gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa
     7681 ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg
     7741 tggcgaaaat gtccgaagtc tacggcatta aacggcgcaa tttccgatac ttacgcgtcg
     7801 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     7861 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     7921 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     7981 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8041 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8101 ttctccatcg gtcaccagca acagtttgca tcatggactg cctataaagt tgcgccgttc
     8161 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8221 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8281 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8341 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8401 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8461 gttctggcga ttgactgtgc ggctatttat agcttcgcgc cgaacgccgt ggtctggggc
     8521 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8581 tcgagcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     8641 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     8701 atcgaaatct ttggttgcgt ctgggcggtg aaacttaccg gtatgagtgc ctggatgggg
     8761 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     8821 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     8881 ctgcgcgcag aagaagatgc agaaaaacaa ctggcagaac agtctgctta ataaggagtt
     8941 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9001 atgaggatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9061 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9121 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9181 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9241 gatgtgaagt aaggacgcgc catgaaatta cgtgattcgc tggcggagaa taaatccatc
     9301 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9361 gtggcggcgg atgttgtcga gccgcgttac taccaggcga ttctggatgc ggttgaacag
     9421 cacggtccgt actttgtgct cgcaccaggc ctggcaatgc cgcacggtcg cccggaagag
     9481 ggcgttaaga aaaccggttt tgcgctggtg acgctgaaaa agccgctgga gttcaaccac
     9541 gaagataacg acccggtgga tatcctcatc accatggcgg cggtcgatgc caacacgcac
     9601 caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag atgaagaaaa tttcgaccgt
     9661 ttacgtgcct gccgtaccga gcaggaagta ctggatctca ttgaccgcac caacgcggca
     9721 gcttaagaag gaattgaaga tgtcattacc gatgttgcaa gtcgcgctgg acaaccagac
     9781 tatggatagc gcctacgaaa ccactcgcct gattgccgaa gaagtcgaca ttatcgaagt
     9841 gggtaccatt ctgtgcgtgg gcgaaggcgt gcgtgcggtt cgcgatctga aagcgctcta
     9901 cccgcacaaa atcgtgctgg cagacgccaa aattgccgat gcgggcaaaa ttctttctcg
     9961 tatgtgcttc gaagccaacg ctgactgggt gaccgtgatt tgttgtgcgg atatcaacac
    10021 cgccaaaggt gcgctggacg tagcaaaaga gtttaacggc gacgtgcaga tcgaactgac
    10081 cggttactgg acctgggaac aggcgcaaca gtggcgcgac gcaggtattc agcaggtggt
    10141 ttatcaccgc agccgtgacg cgcaggccgc tggcgtggcg tggggtgaag ccgatatcac
    10201 tgcgatcaaa cgtctttccg atatgggctt caaagtcacc gtcaccggtg gcctggcgct
    10261 ggaagatctg ccgctgttca aaggcattcc aattcatgtg tttatcgccg gtcgcagcat
    10321 ccgtgatgcc gcttctccgg ttgaagccgc gcgtcagttc aaacgttcca tcgccgaact
    10381 gtggggctaa ggagcggata tgttgtccaa acaaatcccg cttggcatct atgaaaaagc
    10441 gctccccgcc ggggagtgct ggctggaacg cctgcgactg gcaaaaacgt tgggcttcga
    10501 ttttgtcgag atgtcggtag atgaaactga cgagcgcctg tcgcgcctcg actggagccg
    10561 cgagcagcgt ctggcgctgg tgaatgcgat tgttgaaacc ggcgtgcgtg tgccgtccat
    10621 gtgcctttct gcccatcgtc gtttcccgtt gggcagcgaa gatgacgcgg tgcgggcgca
    10681 ggggctggag attatgcgta aagccatcca gttcgcccag gacgttggca ttcgcgtgat
    10741 ccagctggcg ggctatgacg tttactatca ggaagccaat aacgaaacgc gtcgtcgttt
    10801 ccgtgacggc ctgaaagaga gcgttgagat ggcgagccgc gcgcaggtga cgctggcgat
    10861 ggagatcatg gattatccgt tgatgaactc catcagcaag gcgctgggtt acgcgcacta
    10921 tctcaacaac ccgtggttcc agctctaccc ggatatcggc aacctgtcgg cgtgggacaa
    10981 cgatgtgcag atggagttgc aggccggaat cgggcatatc gtcgcggtgc atgtgaaaga
    11041 caccaaaccg ggcgtcttca aaaacgtgcc atttggcgaa ggtgtggtgg atttcgaacg
    11101 ttgcttcgaa acgctcaaac agagtggcta ttgcgggccg tacctgattg agatgtggag
    11161 cgaaacggcg gaagacccgg cggcagaagt ggtgaaagcg cgtgattggg tgaaagcgcg
    11221 catggccaaa gcaggcacgg tggaggcggc ataatgcaaa agctaaaaca gcaggtgttt
    11281 gaagccaaca tggatctgcc gcgctatggg ctggtgacct ttacctgggg caacgtcagc
    11341 gctatcgacc gcgaacgcgg gctggtggtg atcaagccca gcggcgttgc ctacgaaacc
    11401 atgaaagcag acgatatggt ggtggtcgat atgagcggca aggtggtgga aggggagtat
    11461 cgcccgtctt ccgacaccgc gacgtatctc gaactctacc gtcgttaccc gtcgcttggc
    11521 ggcattgtcc atacccactc cactcatgct actgcatggg cgcaggcggg gctggcgatc
    11581 ccggcgttag gcactacgca cgccgactac ttctttggcg acatcccgtg tacgcgcgga
    11641 ttaagcgaag aagaggtgca gggcgagtat gaactgaaca ccggcaaagt gattatcgaa
    11701 acgctgggtg acgccgagcc gctgcatacg ccggggattg tggtgtatca gcacggtccg
    11761 ttcgcctggg ggaaagatgc ccacgacgcg gtgcataacg cggtggtgat ggaagaagtg
    11821 gcgaaaatgg cgtggattgc gcgcagtatt aacccacaac tgaatcacat cgacagcttc
    11881 ctcatgaata aacacttcat gcgtaagcac ggtcctaacg cctattacgg gcagaagtaa
    11941 gcagtgcgcc ccggaattat tacctcctgc gggatgtaat tggtctgatt ccggggccgc
    12001 ttcagttaaa aaatcgtata aacaacaggt gtttttatgg ctatcaatgg attgtttgtc
    12061 agaacggatc cgcaacgtcg ccgctatggc gttgcgttac ttgtgggtgt actttcagga
    12121 attatttcag catttgttaa atggggagcg gaagtcccct tgccaccgcg cacattttcc
    12181 ggtggtcgcg atgaatttaa cccgccgtat atctttctac gggattattt aggtatcgac
    12241 ccaacccaaa ctgtgtatac attctcggaa catattatca acccggtgat ggtgacgcat
    12301 atcatcttct cactggtttt tgcgataggg tattgtattg tcgcggaaat tttcccgaaa
    12361 gtgaaattat ggcaggggat attagcaggg cttattgtga cagtggcagt ccacggtata
    12421 ttttgcccgg cgctgaattt aacgccgccg ctcactcaat tgccttttga cgaatatgcc
    12481 tctgaaatcc tggggcatat tttctggttc tggataatag aaattatgcg ccgcgactta
    12541 cgtaatcgca ttactcatga gccagatccg gaagtcgcaa tccggtgaat aaccgcataa
    12601 aaaagccccc tgcgaagggg gcaaagcaaa ctatggcaat gtttcgttgg ttatacctgg
    12661 tgctagcgat aaatatccgc gctggcgtgc atattgccgt tactccccgg ttcccgcatc
    12721 agaattacgt ggtagtacgt tactccccgg ttcccgcatc agaattacgt ggtagtacgt
    12781 tgcgccctgc gcatctgtct cttcatttaa tgcctgacgc gcttcgcttt cagtggcgaa
    12841 attatgattg atataaatca cgcctaagct ttgcacatca tccatgtttc tggcctggtg
    12901 gttattaatt tcaatggctg cccatgtatt tgcacttagc aaaagcacag ccagaagggc
    12961 taaaactcga ctgaacatag atacctcctc gacggctgac tttgtgtgct ctcctctgtg
    13021 atgatcttct gatttaattt taatcaatga taaagaagtt gatggtgacc atttctgatg
    13081 cagttgttca aaaaaacacc atgatgaagt gtgatgaact ccaaatcagc ggtttagagg
    13141 ttaattgcga aaggggagat ttatttcggc tctgcccttg agtttagcgg ggcatacaag
    13201 tactataacg cgtcattttt cagccgacct ttaacacgtt ccttgcctcc ccgggattcg
    13261 gctgacccag acaggaggct gaataatccg taaggagcaa ttcgatgcgt cattacgaaa
    13321 tcgtttttat ggtccatcct gaccagagcg aacaggttcc gggcatgatc gagcgctaca
    13381 ctgctgccat cactggtgca gaaggcaaga tccaccgtct ggaagactgg ggccgccgtc
    13441 agctggctta cccgatcaac aaactgcaca aagcacacta cgttctgatg aacgttgaag
    13501 ctccgcagga agtgatcgat gagctggaaa ctaccttccg cttcaacgat gccgttatcc
    13561 gcagcatggt tatgcgtacc aagcacgctg ttaccgaagc atctccgatg gttaaagcga
    13621 aagacgagcg ccgtgagcgt cgcgatgatt tcgcaaacga aaccgctgat gatgctgaag
    13681 ctggggattc tgaagagtaa tttctgatga ccaaccgtct ggtgttgtcc ggcaccgtgt
    13741 gcaggactcc ccttcgaaag gtcagtccat caggaattcc tcactgccag ttcgtgcttg
    13801 agcatcgttc tgtgcaggag gaagccggtt ttcaccggca ggcgtggtgc caaatgcccg
    13861 ttattgttag cggacacgaa aaccaggcca ttactcacag tataacggtc ggcagtcgca
    13921 taaccgttca ggggttcatt tcatgccaca aggcaaagaa cggactgagc aaaatggttt
    13981 tgcatgccga gcagattgaa ttgatagatt ctggagacta gccatatggc acgttatttc
    14041 cgtcgtcgca agttctgccg tttcaccgcg gaaggcgttc aagagatcga ctataaagat
    14101 atcgctacgc tgaaaaacta catcaccgaa agcggtaaga ttgtcccaag ccgtatcacc
    14161 ggtacccgtg caaaatacca gcgtcagctg gctcgcgcta tcaaacgcgc tcgctacctg
    14221 tccctgctgc cgtacactga tcgccatcag taatcggtca cggtccatta atacgacttt
    14281 gagaggataa ggtaatgcaa gttattctgc ttgataaagt agcaaacctg ggtagcctgg
    14341 gtgatcaggt aaacgttaaa gcgggctatg ctcgtaactt cctggtaccg cagggtaaag
    14401 ctgttccagc taccaagaaa aacattgaat tcttcgaagc acgtcgcgct gaactggaag
    14461 ctaaactggc tgaagttctg gcagctgcta atgctcgcgc tgagaaaatc aatgcactgg
    14521 aaactgttac catcgcgtct aaagctggcg acgaaggtaa actgttcggt tccatcggta
    14581 ctcgcgacat cgctgacgct gtaactgcag ctggcgttga agtggctaag agcgaagttc
    14641 gtctgccgaa cggcgttctg cgtaccactg gcgagcacga agtgagcttc caggttcaca
    14701 gcgaagtatt cgcgaaagtg atcgtaaacg tagtagctga ataattcgtt attcaacgag
    14761 acgtaaaaac gccgaccagt ggtcggcgtt ttgcttttgt agcgagcact tgcagatatc
    14821 gataagggcc acaataagca gccctacgaa gataattact tagctgcctg aatatcagcc
    14881 agcattgggg caagggctgg gttgtcctta gcgaagtttg cgtacattgg ctgtactttc
    14941 tcgataaacg gagctttatc tacctggtag actttcacgc ccattttatc cagttttgca
    15001 aattcttgtt tctcttcctc gctccacaag tctttcatgt agctcattga ttctttagct
    15061 gcttttaata taactgcctg attttccggg ctaagtttat caaacgcagc attgctcatc
    15121 agaaccacat cgggaaccat agtgtgttcg tcaaggctga aggattttgc cacttcaccg
    15181 tgacgcatgg tggtcagcgc catcacgctg ttttctgcca tatcgaccac accctgctgt
    15241 aaggctgtat acaactcgcc gtaatccagt ggcgtcgggt taccgcccat gactttgatc
    15301 atttcaactg cgccagggct tggctggaca cggactttca tgcctttcag atcgtctggt
    15361 ttcagtactg gtttattgcc atagaaactg cgggcgcctc catcataaaa agtcagcccg
    15421 aaataacctt tgcttttcgt tgagtcgagg atttttctcc cgatatcgcc ctgcatgacc
    15481 tgataataat gatcacgatc gcggaacaaa taaggcaggc taaataattt ataggaattt
    15541 tcaaatgctt ccatttctgc cgcattggtt tttaccagtg gaatagcgcc agaacgaatc
    15601 agctccattg tttctcgttg agtacccaat gttccatttg gataaatacg aatagtaata
    15661 tcaccgttag agtactcttt gttctttttc gcaaagaact ccatcgcttt atgaacagga
    15721 tgagacttat cctgattatg actcagtttt aatgtcactg cccaggactg gcttgcggat
    15781 aacataagaa cagcaccgat aatgccagcg gatactttta ttttcataga tgttcctttt
    15841 ctaataaata tggcggcagg tgccgccagt gaatgcgcat cagtagccca gtgcttttgg
    15901 caatgccata ctgatttgcg gaatataggt caccattgcc agcaccaata ccatcacggc
    15961 atagaatggc agcataggtt tgataatttt attaatcggt aatttagata cactacaacc
    16021 gacaaataac acactgccta caggcggagt acaaataccg atacacatgt tcagtaccat
    16081 gacaatacca aagtgaatcg ggtccatgcc cagttcggtg acaacaggta aaaatatcgg
    16141 cgtaaaaatc agtaccgccg gtgtcatatc cataaaagtg ccgataatca acaggataat
    16201 attgataata agcaggatta ttattgggtt gtcagaaata gtgttaagca aatcgagaat
    16261 caggaaggga acgtcagcgt ttgacattgc ccatgacggt aatgactcca acttattgat
    16321 agtgttttat gttcagataa tacccgatga ctttgtcatg cagctccacc gattttgaga
    16381 acgacagcga cttcc
//
LOCUS       DAETQN010000096        16393 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.096, whole genome shotgun sequence.
ACCESSION   DAETQN010000096 DAETQN010000000
VERSION     DAETQN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.096"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            79..2394
                     /gene="bisC"
                     /locus_tag="K0W68_003083"
     CDS             79..2394
                     /gene="bisC"
                     /locus_tag="K0W68_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBI3074579.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2363..2803)
                     /locus_tag="K0W68_003084"
     CDS             complement(2363..2803)
                     /locus_tag="K0W68_003084"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI3074580.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2800..3363)
                     /gene="tag"
                     /locus_tag="K0W68_003085"
     CDS             complement(2800..3363)
                     /gene="tag"
                     /locus_tag="K0W68_003085"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBI3074581.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3521..4219
                     /locus_tag="K0W68_003086"
     CDS             3521..4219
                     /locus_tag="K0W68_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI3074582.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4448..5656
                     /locus_tag="K0W68_003087"
     CDS             4448..5656
                     /locus_tag="K0W68_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3074583.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5980..7671
                     /gene="eptB"
                     /locus_tag="K0W68_003088"
     CDS             5980..7671
                     /gene="eptB"
                     /locus_tag="K0W68_003088"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBI3074584.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7763..7839
                     /locus_tag="K0W68_003089"
     tRNA            7763..7839
                     /locus_tag="K0W68_003089"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7797..7799,aa:Pro,seq:cgg)
     gene            7874..8007
                     /locus_tag="K0W68_003090"
     ncRNA           7874..8007
                     /ncRNA_class="other"
                     /locus_tag="K0W68_003090"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8752..10359
                     /gene="dppA"
                     /locus_tag="K0W68_003091"
     CDS             8752..10359
                     /gene="dppA"
                     /locus_tag="K0W68_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBI3074585.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10667..11686
                     /gene="dppB"
                     /locus_tag="K0W68_003092"
     CDS             10667..11686
                     /gene="dppB"
                     /locus_tag="K0W68_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBI3074586.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11696..12598
                     /gene="dppC"
                     /locus_tag="K0W68_003093"
     CDS             11696..12598
                     /gene="dppC"
                     /locus_tag="K0W68_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBI3074587.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12609..13592
                     /gene="dppD"
                     /locus_tag="K0W68_003094"
     CDS             12609..13592
                     /gene="dppD"
                     /locus_tag="K0W68_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBI3074588.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13589..14593
                     /gene="dppF"
                     /locus_tag="K0W68_003095"
     CDS             13589..14593
                     /gene="dppF"
                     /locus_tag="K0W68_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBI3074589.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14623..15894)
                     /locus_tag="K0W68_003096"
     CDS             complement(14623..15894)
                     /locus_tag="K0W68_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBI3074590.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3900 a         4087 c         4407 g         3999 t
ORIGIN      
        1 attattcctg ggaatactcg gaaatttgta agtaatattt aactgctcaa tacatctaac
       61 ctttcaggag tcttcggttt ggccaactca tcctcacgat attccgttct gactgccgcc
      121 cactgggggc ccatgctggt tgaaaccgac ggcgaaaccg tgtttagctc gcgtggcgcg
      181 ttagccacag gaatggaaaa ctccttgcag agcgcggttc gcgaccaggt tcacagcaat
      241 acgcgggtac gatttccaat ggtgcgcaaa ggctttctcg cgtcaccgga aaacccgcaa
      301 ggcattcgtg ggcaggatga atttgttcgc gtgagttggg atgaggcgct ggatcttatt
      361 caccaacaac ataaacgcat tcgtgaggct tatggtccgg catcgatttt tgctggttcc
      421 tacggctggc gttcaaacgg cgtgctgcat aaggcctcga cattattaca acgctatatg
      481 gcgctggcag gcggttatac cgggcatctg ggggattatt cgaccggtgc ggcacaggcg
      541 atcatgccgt atgtcgtagg tggcagcgaa gtttatcaac agcaaaccag ttggccgctg
      601 gtgctggaac atagcgatgt cgtggtgttg tggagtgcta acccactcaa tacgctgaaa
      661 attgcgtgga atgcatccga tgagcagggg ctttcttact tttccgcact gcgtgacagt
      721 gggaaaaagc tgatctgcat tgatccaatg cgatcggaaa ccgtcgattt ctttggcgat
      781 aaaatggagt gggtggcacc gcacatgggc accgatgttg cgctgatgct ggggatcgcc
      841 catacgctgg tggaaaatgg ttggcacgac gaagcgtttc tggcgcgttg caccacaggt
      901 tatgccgtct tcgcctctta tttgctgggc gagagtgacg gaatagcgaa aaccgccgaa
      961 tgggcagcag agatttgtgg tgttggcgca gcgaaaatcc gccagctggc ggctattttc
     1021 caccaaaata ccaccatgct gatggcaggc tggggaatgc agcgccaaca gtttggtgag
     1081 caaaaacact ggatgatcgt cacgctggca gcaatgttgg ggcaaatcgg cacacccggc
     1141 ggcggttttg gtctttctta ccattttacg cggcgttctg cggtgctctc ttccatgcag
     1201 ggcagcttgc cgggtggctg cgatgcggtg gataaaatcc ctgttgcccg cattgttgaa
     1261 gcactggaaa accctggtgg cgcatatcaa cacaacggta tgaaccgaca tttcccggat
     1321 attcgtttta tctggtgggc gggcggtgcc aactttactc atcatcagga taccaatcgt
     1381 ctgatccgtg cctggcaaaa accggagctg gtggtgatct ctgaatgctt ctggacggcg
     1441 gcggcgaaac acgcggatat cgttctgcct gcgactacct cttttgagcg taatgatctc
     1501 accatgaccg gtgattacag taatcagcat ctggtgccga tgaagcaagt ggtgccgcca
     1561 cgctatgaag cgcgtaatga ttttgatgtt tttgccgagt tgagtgaacg ttgggagaag
     1621 ggcggctatg cgcggtttac ggaaggaaaa agtcagctgc aatggctgga aacgttttat
     1681 aacgttgccc ggcagcgcgg ggcaagccag caggttgaat tgccgccatt tgctgagttc
     1741 tggcaagcca accagttaat tgagatgccg gaaaacccgg acagcgagcg gtttattcgc
     1801 ttcgctgatt tttgccgcga tccgctggcg catccgttaa aaaccgccag cggcaagatt
     1861 gaaatcttct cacagcgtat tgccgattac ggttacccgg attgccctgg gcatccaatg
     1921 tggctggagc cggacgaatg gcagggcaat gccgaaccag aacagttgca ggtactttct
     1981 gcccatccgg cgcaccgcct gcacagccag ctgaattaca gttctctgcg cgaattgtac
     2041 gcggtagcaa accgtgagcc tgtcaccatt catcctgacg atgctcaggc gcgcggcata
     2101 accgaagggg atatggtgcg ggtgtggaac tcccgtgggc agatccttgc cggagcggtc
     2161 attagcgagg gaattaaacc tggcgtgatt tgcattcacg aaggggcatg gccggatctg
     2221 gatttaaccg ctgacggtat ttgtaaaaac ggcgcagtga acgtgctgac caaagatctc
     2281 cccagctcgc ggctggggaa tggctgtgcg ggtaatacgg cgctggcatg gctggaaaaa
     2341 tacaacggtc cggaactgac acttacagcg tttgaaccac cggccagctc ataatccagg
     2401 tgggtagttg ggtttcatcc tgccatgcgc aatcgacaat gtgaaaaccc tgcgcacggt
     2461 aaaaatctat cgccggttga tttttttgat aaacctccag catcagatgg ggatagcgct
     2521 gctgcacata ctgcatcagc gccttaccaa taccgcgtct gacggccttc ggtgcgacaa
     2581 acattgctgc cagaaatcgg ccttccataa tgctgacaaa accgagaagc ttaccgtcct
     2641 cttcccagac ccagttttgc gcgttggcaa gataggcatc ccgcaccagc ggaatgcagt
     2701 cacgccagta attcgctttt ataaagggat gcccccaggt tgtactttcc agccacagtt
     2761 cgaggatcgc ggggagttct gaacgttgcg cttcccgaat catggtttat ttcccggata
     2821 gcaacagcag ccaaccacat gatcattcac cagcccacat gcctgcataa aggagtaaca
     2881 gattgtggtg ccgacaaact taaaaccacg ttttttcagt gctttagata gggcgtcgga
     2941 ggcggacgta gatgtgggaa tttcgctcaa cgttgtggct tgtgtcacct gtggctgatg
     3001 atttacaaac gaccagacaa agtcggcaaa cggttcgccg ttctgttcca tttgcaggta
     3061 cgcccgcgca ttaccaataa ttgcctgaat tttccctcga tggcggataa tcccggcgtc
     3121 ctgtaccagt ctttcgacat cctcttcctg cattgctgcg accttcaccg gatcgaactg
     3181 atgaaagcag gcgcgatagt tttcgcgttt tttgaggacg gtgatccacg ataacccagc
     3241 ctgctgccct tcaaggcaga tcatttcgaa cagtttttta ctgtcagttt caggcacgcc
     3301 ccattcatta tcatggtagg caatataaag cgggtcctga ctcacccagc cgcaacgttc
     3361 catactttcc ctcgctatgt acccgatcgt aaaaatttca gccgcttttc ttgacgcatc
     3421 ggtgaaaaag tcaatattta atacagggct actatgcccg atatccttca tacaatgaca
     3481 ataatattgc cgaattcggc tcttccctgg agtcgtgtta atgatcataa aaaaaagcgg
     3541 tggtcgctgg cagctaagcc tgctggcgag cgtggtaatc agtgcctttt ttctcaacac
     3601 agcttacgcc tggcaacaag aatatatcgt tgatacgcaa cccggacatt ccacagagcg
     3661 ttacacctgg gatagtgatc atcaacctaa ttacaacgat attttgtcgc aacgtattca
     3721 aagtagccaa agggcgctgg gactggaagt caatctggcg gaagaaactc ctgtggatgt
     3781 gaccagcagt atgagtatgg gctggaattt tcctttatat gaacaggtta caaccggccc
     3841 ggtcgcggca ttacattacg atggcacaac cacctcgatg tataacgagt ttggcgacag
     3901 tactaccacg cagaccgatc cgttatggca tgccagcgtg agtaccttag gctggcgtgt
     3961 tgactcccgg cttggcgatc tccgaccctg ggcgcaaatc agctataacc agcaatttgg
     4021 cgagaatatc tggaaggcgc aatcaggcct gagccggatg acggcgacaa accagaacgg
     4081 caactggctg gatgtcaccg taggcgctga tatgttgctc aatcaaaata ttgccgccta
     4141 tgccgcgcta actcaggcag aaaataccac taataatagc gactatctgt atacgatggg
     4201 ggttagcgcc agattttaac gtaacagtca caattgaaac cattaaataa caataattgt
     4261 ggcgatagtg ggtgctaact taccaaataa taaatttggt gaataattgt cgcgtcattc
     4321 attcctgaac taaggcattt cattccgttc tgatggcatt tcatgccgtt tttccccagg
     4381 cataaagtgc acttcgttat ggttgtcggc agagattttt cctttttatt actgcaggaa
     4441 tactgccatg acaccttcaa attatcagcg tacccgctgg ctgacactca tcggtactat
     4501 cattacccag tttgcgctgg ggtcggttta tacctggagc ctgtttaatg gcgcgctttc
     4561 cgccaagctg gatgcgccgg taagccaggt cgctttctct ttcggcttgt taagtctggg
     4621 gctggcaatt tcgtcttctg ttgcgggcaa attacaggaa cgtttttgcg ttaaacgcgt
     4681 caccatggct tccggcattt tgctgggatt aggcttcttc ctgaccgcgc attccaacaa
     4741 cctgatgatg ctgtggttaa gcgccggtgt gctggtcggt ctggcggatg gcgcgggtta
     4801 cctgctgacg ctctctaact gcgtgaagtg gttcccggag cgtaaaggtc tgatctccgc
     4861 gttcgctatc ggttcttatg gtctgggtag cctgggtttc aaatttatcg acacgcagct
     4921 gctcgaaacg gtgggtctgg aaaaaacatt tgtgatttgg ggagcgattg cgctggtgat
     4981 gattgttttc ggcgcaacgt taatgaaaga cgcaccaaaa caggaagtga aaaccagcaa
     5041 tggtgtggtg gagaaagatt acacgctggc agagtcgatg cgtaaaccgc agtactggat
     5101 gttagcggta atgttcctga ctgcctgcat gagcggcctg tacgtgattg gggtagcgaa
     5161 agatatcgcc caaagtctgg cacaccttga tgtggtttcc gcagccaatg cagtaacggt
     5221 tatttccatc gctaaccttt caggtcgtct ggtgctgggc attctgtctg acaaaattgc
     5281 ccgtatccgt gttatcacca ttggtcaggt gatttcgctg gtgggtatgg cggccctgct
     5341 gtttgcacca ttaaatgcag tgacgttctt tgcagcgatt gcctgcgtgg catttaactt
     5401 tggcggcact attaccgtct ttccatcact ggtcagtgag ttcttcggcc tcaataacct
     5461 ggcgaaaaac tacggtgtga tttacctcgg tttcggtatc ggtagcattt ttggttcgat
     5521 tatcgcctca ctgtttggcg gtttctatgt gaccttctac gtgattttcg ccctgctgat
     5581 tctgtcattg gcgctttcta cgacgattcg tcagccagag cagaaaatgt tgcgtgaggc
     5641 gcatggctcc ctttaatgct cagccgcgag ctactgggct tcagcttccg tgtgcatcaa
     5701 acgcatcaac gaaatgtata aacaatgact cgtaaaattt gggccagtta actggccctt
     5761 tttactgctt tttgtaacgc taaagtctct tttcaaactt gcatttttgt aaatttgtgc
     5821 ttcatgcaca ctctttcccc acactttttc gctttgctgt ggtctactta ttcgcgcgtg
     5881 tagattttac ttatctgact acctccgcac ttttcccctg ccgggcctga aaagccgtta
     5941 agcagggtgt tatcacctgt ttgtccaggg tttgtttgca tgagatacat caaatcgatt
     6001 acacagcaga agctgagctt tttgcttgca atctatattg gcctttttat gaatggcgcg
     6061 gttttttatc gccgcttcgg cagctatgcg cacgatttta ccgtctggaa aggcgtttct
     6121 gctgttgttg aactggccgc caccgtactg gtgaccttct ttttactacg tcttctatcg
     6181 ctgtttggcc gccgcagctg gcgtattctg gcgtcgctgg tggtgctctt ttccgcaggt
     6241 gccagctatt acatgacctt ccttaatgtg gtcattggtt atggcatcat cgcttccgtc
     6301 atgaccaccg atatcgacct gtcaaaagaa gttgttggtc tgaactttat tctctggtta
     6361 atcgccgtta gtgcattgcc tcttatcctt atctggaata accgctgtcg ctacaccttg
     6421 ctccgacaac tgcgaacccc agggcagcgt attcgcagcc tggcggtcgt cgtactggcg
     6481 ggtattatgg tttgggcacc gattcgtttg ctggatatcc agcagaagaa agtggagagg
     6541 gcgaccggcg ttgatttgcc gagttatggc ggtgtcgtag cgaactctta tctgccatca
     6601 aactggcttt ctgcgttggg gctgtatgcc tgggcgcggg tcgatgaatc ttccgataat
     6661 aattcattgc ttaatccggc gaagaagttt acataccagg ctccacaaaa cgttgatgag
     6721 acttatgtcg tgtttatcat cggtgaaacc acgcgttggg accatatggg tattttcggc
     6781 tatgagcgta ataccacgcc gaaactggcc caggagaaaa atctggcggc gttccgtggt
     6841 tactcctgtg ataccgcaac caaactctca ctgcgttgca tgtttgtacg tcaggggggc
     6901 gcggaagata atccgcagcg cacattaaaa gaacagaaca ttttcgcggt tctgaagcag
     6961 ttaggattca gttctgacct ctacgctatg cagagcgaaa tgtggttcta cagcaacacg
     7021 atggcggaca acattgctta tcgtgagcag attggtgcgg agccacgtaa tcgtggcaag
     7081 ccggtagatg atatgttgct ggtagacgaa atgcagcaat cgctagggcg caacccggat
     7141 ggtaagcatc tgatcattct gcataccaaa ggttcgcatt ttaactacac ccagcgttat
     7201 ccgcgtagct tcgcgcagtg gaagccggaa tgtattggtg ttgatagcgg ctgtaccaaa
     7261 gcgcagatga tcaactccta tgacaactcg gtgacctatg tggatcactt tatctccagc
     7321 gtgattgatc aggttcgcga taagaaagcg attgtgttct acgcagctga ccacggtgag
     7381 tcaattaatg aacgcgagca cctgcacggc acgccgcgtg aactggcacc gccggagcag
     7441 ttccgcgtac cgatgatggt ctggatgtca gataaatatc tggaaaatcc ggccaatgcg
     7501 caggcgtttg cgcagctgaa aaaagaagcc gacatgaaag tgccacgccg tcacgtagag
     7561 ctgtacgaca ccatcatggg ttgtcttggc tatacttcac cggatggtgg aattaacgaa
     7621 aacaacaact ggtgtcacat cccgcagaca aaagaggcag cggctaacta agaggcctgc
     7681 tgactttctc gccgatcaaa aggcattttg ctattaaggg attgacgagg gcgtatctgc
     7741 gcagtaagat gcgccccgca ttcggtgatt ggcgcagctt ggtagcgcac ttcgttcggg
     7801 acgaaggggt cggaggttcg aatcctctat caccgaccaa attcgaaaag cctgctcaac
     7861 gagcaggctt ttttgcattt gtagctcatg aggatgagaa cctccggggg cagggaggtt
     7921 cgactcgagc gaagcgagag aacgttgcgc cagcaacggc ccgcagggcg agccacgaag
     7981 tggcgagtaa tcctctatca ccgaccaaat tcgaaaagcc tgctcaacga gcaggctttt
     8041 ttgcatttgt agctcatgag gatgagaacc tccgggggca gggaggttcg actcgacgta
     8101 ggcctgataa gacgcgccag cgtcgcatca ggcaacggca ccgcatttat tgtcggatgc
     8161 ggcgcgaccg ccttattcga ccaacacacc acacattttc ctgccaactt tgcgccccat
     8221 atctcactat ccattaacgg atttgtgaca gaaaccattg tcttttttta tatttgctta
     8281 aatctttttt tgcgttactt atggctggcg ctggcatttt ccgctgtgcg ttttagcgtt
     8341 catggtatta atcgctcaga tatccacctt ttcctcagat ttttttcatt aaatgcataa
     8401 aacttaatca ccaaatgttg ctaaatatta agctgattgt tctggttatg ttggagtagc
     8461 acaaatttct ctgctgcaaa tagctgtttg aagttttttt aatctttgtt tgtggattct
     8521 caccgttggt gtaaatcccg gttggctgta ttgacgtttt cacattctgt tgacagattg
     8581 taggtcacga ggggcatttt atggaggatc cgcactgtta cactgatgtt aattagtacg
     8641 gcatccccac ctcataacgt tgacccgacc gggcaaaaaa caaaaaaggt caggcagcga
     8701 caacccactg caaagggtta aaacaacaaa catcacaatt ggagcagaat aatgcgtatt
     8761 tccttgaaaa agtcagggat gctgaagctt ggtctcagcc tggtggctat gaccgtcgca
     8821 gcaagtgttc aggctaaaac tctggtttat tgctcagaag gatctccgga agggtttaac
     8881 ccgcagctgt ttacctccgg caccacctat gacgcctctt ccgtaccgct ttataaccgc
     8941 ctggttgaat ttaaaatcgg caccactgaa gtgatcccgg gcctcgctga aaagtgggaa
     9001 gtcagcgaag acggtaaaac ctataccttc catctgcgta aaggtgtgaa gtggcacgac
     9061 aataaagaat tcaaaccgac gcgtgaactg aacgccgatg acgtggtgtt ctcgttcgac
     9121 cgtcagaaaa acgcgcaaaa cccgtaccat aaagtctctg gcggcagcta cgaatacttc
     9181 gaaggcatgg gcttgccgga gctgatcagt gaagtgaaaa aggtggacga caacacagtt
     9241 cagttcgtac tgactcgccc ggaagcgccg ttcctcgctg atctggcaat ggacttcgcc
     9301 tctattctgt caaaagaata tgctgatgcg atgatgaaag ccggtacacc ggaaaaactg
     9361 gacctcaacc caatcggaac cggtccgttc cagttacagc agtaccaaaa agattcccgt
     9421 atccgctaca aagcgtttga tggctactgg ggcaccaaac cgcagatcga tacgctggtt
     9481 ttctctatta cccctgatgc ttccgtgcgt tacgcgaaat tgcagaagaa tgaatgccag
     9541 gtgatgccgt acccgaaccc ggcagacatc gctcgcatga agcaggataa atccatcaac
     9601 ctgatggaaa tgccggggct gaacgtcggc tacctctcct ataacgtgca gaaaaaaccg
     9661 ctggatgacg tgaaagttcg ccaggcactg acctacgcgg tgaacaaaga cgcgatcatc
     9721 aaagcggttt atcagggcgc gggtgtatca gcgaaaaacc tgatcccgcc aaccatgtgg
     9781 ggctataacg acgacgttca ggattacacc tacgatcctg aaaaagcgaa agccttgctg
     9841 aaagaagcgg gtctggaaaa aggtttctcc atcgacctgt gggcgatgcc ggtacaacgt
     9901 ccgtataacc cgaacgctcg ccgcatggcg gagatgattc aggcagactg ggcgaaagtg
     9961 ggcgtgcagg ccaaaatcgt cacctacgaa tggggtgagt acctcaagcg tgcgaaagat
    10021 ggcgaacatc agacggtaat gatgggctgg actggcgata acggggaccc ggataacttc
    10081 ttcgccaccc tgttcagctg cgccgcctct gaacaaggtt ctaactactc aaaatggtgc
    10141 tacaaaccgt ttgaagatct gattcaaccg gcgcgtgcta ccgacgacca taacaaacgc
    10201 gttgaactgt acaagcaagc gcaggtggtg atgcacgatc agactccggc actgatcatc
    10261 gctcactcca ccgtgtttga accggtacgc aaagaagtca aaggctatgt ggttgatcca
    10321 ttaggcaaac atcacttcga aaacgtctct atcgaataat taaaagccat acaagactga
    10381 tggcaaaggc aaaactgcct gatgtgctcc gcttatcagg cctacgaaaa ttctgcaatg
    10441 tattgaattt gcacgatttt gtaggccgga taaggcgtta acgccgcatc cggcataaac
    10501 aaagcgcact ttgtcaacaa tctgtatacc cggtggcgat gtgcctctgc gtgcagcgcc
    10561 atccggcagc aatacttcat tccctgtccg ataagggcgg gaattgattt gtgagcaata
    10621 cagacacgca gttccaggct gcgggtcact atagagaatc cgggttatgt tgcagtttat
    10681 tctccgacgt ttgggactcg tcatccccac gtttatcggt attacccttc tcacatttgc
    10741 ctttgtccac atgatcccgg gcgatccggt gatgatcatg gcaggcgaac gcgggatctc
    10801 ccctgagcgt cacgcgcagc tgctggctga actcggctta gataaaccga tgtggcagca
    10861 gtatctccat tacatttggg gcgtaatgca cggcgatcta ggcatttcaa tgaaaagccg
    10921 aattccagta tgggaagagt tcgtgccgcg ctttcaggcc acgctggaac ttggcgtctg
    10981 cgcgatgatt tttgctacgg cagtcggtat tccggttggt gtgctggctg cggttaaacg
    11041 cggttccatt ttcgatcaca ctgcagttgg cctggcgctg accggctact cgatgccgat
    11101 cttctggtgg ggcatgatgc tgatcatgct ggtttcggtg cactggaacc tgacgcccgt
    11161 ctccggtcgc gtgagcgata tggtgttcct cgatgactcc aatccgttaa ccggttttat
    11221 gctgatcgac accgccatct ggggtgaaga tggcaacttt atcgacgccg ttgcccatat
    11281 gatcttaccg gctattgtgc tgggtactat cccgctggcg gttatcgttc gtatgacgcg
    11341 ctcttcgatg ctggaagtgc tgggtgaaga ttacatccgc accgcgcgcg ccaaagggtt
    11401 gacccgcatg cgggtgatta tcgtccatgc gctgcgtaac gcgatgctgc cggtagtgac
    11461 cgttatcggc ctgcaggtgg gaacattgct ggcgggggcg attctgaccg aaaccatctt
    11521 ctcgtggccc ggtctgggac gctggttgat tgacgcactg caacgccgcg actatccggt
    11581 agtgcagggc ggcgtattgc tggtggcgac gatgattatc ctcgtcaacc tgctggtcga
    11641 tctgctgtac ggcgtggtga acccgcgtat tcgtcataag aagtaagggg acatcatgtc
    11701 acaggttact gaaaataaag tgattagcgc accggtgccg atgaccccgt tacaggagtt
    11761 ctggcactat tttaaacgca acaaaggcgc ggttgtcggg ctggtttaca tcgtcatcgt
    11821 gctgtttatc acgatctttg ccaactggat tgcgccttat aacccggcgg atcagttccg
    11881 cgatgcgctg ctcgccccgc cagcctggca ggaaggcggc agcatggcgc acttgctggg
    11941 caccgatgac gtaggccgtg atgtgctgtc gcggctgatg tacggcgcgc gcttgtcgct
    12001 gctggttggc tgtctggtgg tggtgttatc gctgattatg ggcgttattc tcggcctgat
    12061 cgccggttac ttcggcggcc tggtcgataa catcatcatg cgcgtggtcg atatcatgct
    12121 ggcgctgcca agtctgctgt tggcactggt gctggtggcg attttcggcc cgtcgattgg
    12181 taacgccgcg ctggcactga ccttcgttgc cttgccgcac tatgtgcgct taacccgcgc
    12241 cgccgtgctg gtggaggtga atcgcgatta cgttaccgcg tctcgcgtgg caggtgccgg
    12301 ggcgatgcgt cagatgttta ttaacatctt cccgaactgc ctggcgccgc tgattgttca
    12361 ggcgtcgctc ggtttctcta acgccattct cgatatggcc gcccttggct tcctcggcat
    12421 gggagcgcag ccgccaacac cagagtgggg caccatgctc tccgacgtgt tgcagttcgc
    12481 gcaaagcgcc tggtgggtcg tgaccttccc gggtctggcg atcctgctga cggtgctggc
    12541 atttaacctg atgggtgacg gtctgcgtga cgcgctcgat cccaaactga agcagtaaga
    12601 ggttcgagat ggcgttatta aatgtagata aattatcggt gcatttcggc gacgaaagcg
    12661 caccgttccg cgccgtagac cgcatcagct acagcgtaaa acagggcgaa gtggtcggga
    12721 ttgtgggtga gtctggctcc ggtaagtcgg tcagttcact ggcgattatg gggctgattg
    12781 attatccggg ccgcgtaatg gcggaaaagc tggtgtttaa cggtcaggat ttgcagcgta
    12841 tatcagaaaa agagcgccgc aacctggtgg gtgccgaagt ggcgatgatc ttccaggacc
    12901 cgatgaccag ccttaacccg tgctacaccg tgggtttcca gatcatggaa gcgattaagg
    12961 tgcatcaggg cggtaataag agcactcgcc gtcagcgggc gattgacctg ctgaatcagg
    13021 tcggtattcc cgatccggca tcgcgtctgg atgtttaccc gcatcagctt tccggcggca
    13081 tgagccagcg cgtgatgatc gccatggcga ttgcctgtcg gccaaaactg ctgattgccg
    13141 atgagccgac cactgcgctg gacgtgacca ttcaggcgca aatcatcgaa ctgttgctgg
    13201 agctacagca gaaagagaac atggcgctgg tgttaattac ccatgatctg gcgctggtgg
    13261 cggaagctgc gcataaaatc attgtgatgt atgcaggcca ggtggtggaa acgggcgatg
    13321 cgcacgccat cttccatgcg ccgcgtcacc cgtatactca ggcattgctg cgtgcgctgc
    13381 cagaatttgc tcaggacaaa gaacgtctgg cgtcgttgcc aggtgtcgtt cccggcaagt
    13441 acgaccgccc gaacggctgc ctgcttaacc cgcgctgccc ctatgccact gacagatgtc
    13501 gcgctgaaga accggcgctg aatatgctcg ctgacgggcg ccagtccaaa tgccattacc
    13561 cacttgatga tgccgggagg ccgacactat gagtacgcaa gaggccaccc tgcaacaacc
    13621 gctgttgcag gctatcgacc tgaaaaaaca ttatccggtg aagaaaggca tgttcgcgcc
    13681 ggaacgtctg gttaaagcgc tggatggcgt ttcgtttaac cttgaacgtg gcaaaacgct
    13741 ggcagtagtg ggcgaatctg gctgcggtaa atcgaccctc ggtcggttgc tgacgatgat
    13801 tgaaacgccc accggtggcg agctgtatta ccaggggcag gatctgctta agcacgatcc
    13861 gcaggcgcag aagctgcgtc ggcagaaaat ccagatcgtc ttccagaacc cttacggttc
    13921 gctgaatccg cgtaaaaaag tcgggcaaat tcttgaagag ccgctgctga tcaacaccag
    13981 cttaagcaaa gaacagcgtc gggaaaaagc cctgtcgatg atggcgaaag tcggcctgaa
    14041 aaccgagcac tatgaccgct atccgcatat gttctccggc ggtcagcgtc agcgtatcgc
    14101 catcgcccgt ggtctgatgc tcgacccgga tgtggtgatt gccgatgaac cggtttccgc
    14161 gctggatgtt tcagtgcgcg cgcaggtgct gaatctgatg atggatttgc agcaggagtt
    14221 ggggctgtct tatgtcttta tctcccacga cctgtcggtg gtggagcaca ttgctgatga
    14281 agtgatggtg atgtacctgg gccgctgcgt ggagaaggga acgaaagacc aaatcttcaa
    14341 taacccgcgc catccgtaca ctcaggcgct actttccgcg acgccgcgcc tgaacccgga
    14401 cgatcgccgc gagcgcatca agctcagcgg tgaactacca agcccactga atccaccgcc
    14461 gggttgcgcc ttcaacgccc gctgtcgtcg gcgcttcggc ccctgcaccc agttgcagcc
    14521 gcagctaaaa gactacggcg gtcaactggt agcttgtttt gctgttgatc aggatgaaaa
    14581 tccgcagcgt taactcacaa aaccggggcc atgcgctccg gtttactcaa tcagcttcaa
    14641 cagtggcgac aagcatagca aaatgccgta cagcagaatc agccaggctt taaatccgcg
    14701 aagtttttcc agttgtgcga ctttatagat caggaaaaac ggaatgaggc acgacacaat
    14761 accatataac gggcttccca actgaaagaa caccagcacc gatacacgaa acgaaaccca
    14821 aatcgtcaac gtaatgacga taaaagcgca gatcgccaga gtcagcacgc gtgagttaat
    14881 tttcttggta tcaataattc ggcttaacag attgagaata atgcctttaa tggcctcgtg
    14941 gaaaccgaga taaatgccaa agaatgcggt cagtacggca aagatattaa gcaccgtaga
    15001 ggtgatatga atgatatgcc cagggatcac ctgcgcggcc agcgccagtg ctgagatatt
    15061 ttgttcaaag gcagaaacgg cttcttcgtg gctaattgag aaggtaaacg aaaaggcaaa
    15121 aaacaggatc accgcgatga gcgtgatata actaattcgg tgggtacgca gcgcgagccg
    15181 ggttgccagt actttatccg cttcccgttt acgataggca atattcattg ggttaagtac
    15241 ctgaataaat actgcagaaa agaagcaaaa tggaatggta agcaagacat cgcggaaaaa
    15301 gacggaggct tgcgggaagg cggttatatt ggcgaaattc cagtgcggga tcatcgcaaa
    15361 accgaacacg acaataatcc ctactttgac caccaccatt ggcccggaaa tcttaaacag
    15421 taatcgttca ccaccagacg caatcgctac cagtacggcg aaaatagcga ctttataaag
    15481 tagagattgt gaaagatcgg catcggttaa accgaaggtt ttcaggtacg aggcgctgtc
    15541 gaaaaccacg gagagagagt agataaaaat cccgtggata atcatcaaaa agtagataac
    15601 cccgaggaaa attccccagt tcttccccag gtaatgactg ataatatcgg tgtagtcatt
    15661 acaggaatcg ctttcagaaa gggtttttaa ataaatgtcc tgcactaccc aggtggcagg
    15721 ataagcaatg atcgctgcgg taataaatac ccaaattccc ttcaagccaa tttgtactgg
    15781 catcagcacg gttccggcac caatcgccat gcctatgcat aataaaaccc agccgaagtc
    15841 gtagcgtgta aacggcaatt tctgattgtg tttcgatagt gtgttgtgct gcataaagtc
    15901 ctgtccagaa agaagtcggc ctcgcaaaaa tgaataaacc cagtttctct tgcgcaacag
    15961 gtggagaagt cgaatacgca gtatgtaaaa aatgaggcaa cagattcact ggggaggtcg
    16021 cgaggaggcg caattctaca aaattgcgca tttcactacc agtctggagt cagcccggat
    16081 atgaactgca actattgcaa ataaagaaat taagctggaa ggcatcgcgc aatgaggaag
    16141 attgccgggt gctgacagta aaaagcaatc gtaaaatcag cggcaagccg aaagggttcc
    16201 ggtgaggcgc aatgttgcgg gggctttatc cctggtggca ttggttgctg gagagagaaa
    16261 acccccgcac gttgcaggta tgtacctgac aacataacgg gggctaatct tgaacctaga
    16321 ccactcaaga atagccgcga aacgttgtca ttacaacaca ggcggctata tgacgctcgc
    16381 agagctgggc atg
//
LOCUS       DAETQN010000097        16070 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.097, whole genome shotgun sequence.
ACCESSION   DAETQN010000097 DAETQN010000000
VERSION     DAETQN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16070
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.097"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..563)
                     /locus_tag="K0W68_003097"
                     /pseudo
     CDS             complement(1..563)
                     /locus_tag="K0W68_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(797..2217)
                     /gene="espX1"
                     /locus_tag="K0W68_003098"
                     /pseudo
     CDS             complement(797..2217)
                     /gene="espX1"
                     /locus_tag="K0W68_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            complement(2702..2965)
                     /gene="rpsT"
                     /locus_tag="K0W68_003099"
     CDS             complement(2702..2965)
                     /gene="rpsT"
                     /locus_tag="K0W68_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBI3074591.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            3068..3286
                     /locus_tag="K0W68_003100"
     CDS             3068..3286
                     /locus_tag="K0W68_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBI3074592.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            3294..4235
                     /gene="ribF"
                     /locus_tag="K0W68_003101"
     CDS             3294..4235
                     /gene="ribF"
                     /locus_tag="K0W68_003101"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBI3074593.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQKEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            4278..7094
                     /gene="ileS"
                     /locus_tag="K0W68_003102"
     CDS             4278..7094
                     /gene="ileS"
                     /locus_tag="K0W68_003102"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBI3074594.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            7094..7588
                     /gene="lspA"
                     /locus_tag="K0W68_003103"
     CDS             7094..7588
                     /gene="lspA"
                     /locus_tag="K0W68_003103"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBI3074595.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            7713..8162
                     /gene="fkpB"
                     /locus_tag="K0W68_003104"
     CDS             7713..8162
                     /gene="fkpB"
                     /locus_tag="K0W68_003104"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBI3074596.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            8164..9114
                     /gene="ispH"
                     /locus_tag="K0W68_003105"
                     /gene_synonym="lytB"
     CDS             8164..9114
                     /gene="ispH"
                     /locus_tag="K0W68_003105"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBI3074597.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            9180..10094
                     /gene="rihC"
                     /locus_tag="K0W68_003106"
     CDS             9180..10094
                     /gene="rihC"
                     /locus_tag="K0W68_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBI3074598.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            10261..11082
                     /gene="dapB"
                     /locus_tag="K0W68_003107"
     CDS             10261..11082
                     /gene="dapB"
                     /locus_tag="K0W68_003107"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBI3074599.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
     gene            11538..12686
                     /gene="carA"
                     /locus_tag="K0W68_003108"
     CDS             11538..12686
                     /gene="carA"
                     /locus_tag="K0W68_003108"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBI3074600.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            12704..15925
                     /gene="carB"
                     /locus_tag="K0W68_003109"
     CDS             12704..15925
                     /gene="carB"
                     /locus_tag="K0W68_003109"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBI3074601.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
BASE COUNT         3922 a         3781 c         4253 g         4114 t
ORIGIN      
        1 tccccccagt agaaaggaat ggagataaac agattaaaac gctcctcttc tttattattt
       61 tcatcataag attggctggc agagaaagta taactaatgt gttgccagtt attggagtaa
      121 ctgaattggt aatctttagc atttccactt cgcccccagt aattccgcca aagcgcactt
      181 aatgatacat ttcccagatt attggataaa ggttgcataa tattggcaga aaggctattt
      241 tttctgccaa tatcataaaa atcatcataa tcactctgat gatagtgttt atcattttcg
      301 taaagatggt cgctgaatgt cctgtaatcc tgcgaagcat aacgccaggc ggccacgcta
      361 aatcgagtgg ctgtttgtac tagatattta ttataggcga cctgatagct cgtaccttcg
      421 tgtctggtat cattatttaa cttactactc gatcgggcag cgtaaaaaga gatggcaccc
      481 agcggtgtat tccaaccatt tcccagagtt atagcattat agttatcgga taaaatcgta
      541 cccgattatg tgatcaggtt gttattattt agattaatgt ttaaattttt taatttcttg
      601 atgtgaagtt gatgaaagtt tgaaaattta atattcttaa ttgttccctt ctttataaga
      661 agagcaaggt gccatcatta gagtgacaga gtaggatgtt tcagagcatg cttcagattg
      721 ttttttattt ctctgcagtg tattaataat catcacagat aatttcattc taaaattacc
      781 tctttctttg agtgttttaa tcctgatgaa ctatattttt tataaggagc atgcactcgt
      841 tgatgctctg aaggtatttt ctcatttttg atgatgtttt gtcgtaatcg tgcataaact
      901 tattaagttc tttatacaca tcattattta gctttacatt atcgcgaact aaagtttgta
      961 ccaatggtat caaaatttcg aaacgggacg gtttgtcatc aatacttgcc agggttttga
     1021 atactcctgt gtatctgtaa cctaagtgct tattgacaag ttgagtggtc catcggccag
     1081 gcataatatc ttcacagcgc aaatttttta tcatggtatt agtgaacgat gttccaaata
     1141 tgttgctttt tttaatgaag aggttgttta tttctccatt tgaaaagatg gtgtttgata
     1201 agtcgctttc aaaacatgat atattggtta atacacaaca aaaataatac ccatatgaaa
     1261 ggttgctctt ttctattgtt atatttgata gcaacatttt atgtatatta atattaagag
     1321 cagtcaaata ctggatcttt tgatcgttaa gttcatacat ctccagtgat ttattatagg
     1381 gtaataaaag tataaattcc tttatattat ctccgcagta agaaaagacg acattaaatt
     1441 tattagtggc catgttttca gttgagcagt aagctgattt ttctattaac cgactgggca
     1501 cgcttttaaa taaagataat tttccacata gttttgaata tgccgaattg ctagttttaa
     1561 agttctgcca cattattccc tggttatcat ctatcgatga ggaggggatt ttattgcatt
     1621 gctcattgta tagctcaggg aaaatatttt ttttaataag ctttttgatt tcctggttaa
     1681 gatgactttt tttcttgtct attgcactgt attttgcgtt ttgtgacgtt tcctgatgat
     1741 cataaaatga ctggagattt ctgcaggcct cataaaatat ttcaggagta tatagctgtg
     1801 ggtatatttc ttggttgaag tttgccttta aaaaattaac gaatttttca tggcattctt
     1861 ggtatatctt aatctcttca gaaagacatt cgcttgttct gtcatcaaaa acatccaatc
     1921 taagtaatgt gatacctttg tgtttaaaaa ataaaattga actgtatgtt ctatgagttt
     1981 acatctataa aaatatgcat ctctcagatg agtatattga aaaaaatgat gaaggactgg
     2041 agatggttta ggcaagttat ggcttctaaa aaagtcatca aacaaaatta agaactctgt
     2101 tttatactcg caaaatactt ttactaactc attttgtctt gttttattta ttattacatc
     2161 atctactata tttgatttat aaagacaatg tggcgaagtt gagataccat ttgtcattat
     2221 cagtccttga agttgatgaa tttttattaa tttaatttat gagtgaatgt gactactatc
     2281 aaaaatagct cattaatttt atgaagtaga atataaatag gtatttaacc tgtgttgatt
     2341 gttgttaatt atttacaaga tctaataagg agatatttat gaatggttaa agtgtatagg
     2401 aatttaattc aaaaatagag tggtcttaag tttaatacct caatgtgtat cacaatatcc
     2461 acattctttg taggggagtc tggagattga gtagatattc ttgttcagaa tgtatcagcc
     2521 gatggttcta cgattcttaa gccacgaaga gttcagaaag tgtaacggca tgtctgcaaa
     2581 agccagcatc ttttgactat ctggcaaccg gtagtgtgtt ctctcacgca tcataaaagc
     2641 agcaggcata aaaaaaaccc gcttgcgcgg gctttttcac aaagcttcag caaattggcg
     2701 attaagccag tttgttgatc tgtgcagtca ggttagcctt atgacgtgca gctttgtttt
     2761 tgtggatcag acctttagca gcctgacggt ccacgatcgg ttgcatttcg ttaaatgctt
     2821 tctgtgcagc agctttgtcg ccagcttcga tagctgcgta tactttcttg atgaaagtac
     2881 gcatcataga gcgacggctt gcgttgtgct tacgagcctt ttcagactga atggcgcgct
     2941 tcttagctga tttgatatta gccaaggtcc aactcccaaa tgtgttctat atggacaatt
     3001 caaaggccga ggaatatgcc cttttagcct tcttttgtca atggatttgt gcaaataagc
     3061 gccgttaatg tgccggcact cgttacgtag tgatggcgca ggattctacc agcttgcggg
     3121 gtgtgaatac agcttttccg cgataaaaat tgcagcaggc ggtcactttc ttcccgtgat
     3181 ttgcgccatg gcaatgaaaa gccacttctt tctgatttcg gtactcaatc gccggttaac
     3241 cttgaccgct gtacaaggta tactcggacg attttcactg ttttgagcca gacatgaagc
     3301 tgatacgcgg catacataat ctcagccagg ccccgcaaga agggtgtgtg ctgactattg
     3361 gtaatttcga cggcgtgcat cgcggtcatc gcgcgctgtt acagggcttg cagaaagaag
     3421 ggcgcaagcg caacttaccg gtgatggtga tgctttttga acctcaacca ctggaactgt
     3481 ttgctaccga taaagccccg gcaagactga cccggctgcg ggaaaaactg cgttaccttg
     3541 cagagtgtgg cgttgattac gtgctgtgcg tgcgtttcga caggcgtttc gcggcgttaa
     3601 ccgcgcaaaa tttcatcagc gatcttctgg tgaagcattt gcgcgtaaaa tttcttgccg
     3661 taggggatga tttccgcttt ggcgctggtc gtgaaggcga tttcttgtta ttacagaaag
     3721 ctagcatgga atacggcttc gatatcacta gtacgcaaac tttttgcgaa ggtggcgtgc
     3781 gtattagcag caccgctgtg cgtcaggcgc tggcggatga caatctggct ctggcagaaa
     3841 gtttactggg gcacccgttt gccatctccg ggcgtgtagt ccacggtgat gaattagggc
     3901 gcactatagg tttcccgacg gcgaatgtac cgctacgccg tcaggtttcc ccggtgaaag
     3961 gggtttatgc ggtagaagtg ctgggcctcg gtgaaaagcc gttacccggc gtggcaaaca
     4021 tcggaacacg cccaacggtt gccggtattc gccagcagct ggaagtgcat ttgttagatg
     4081 ttgcaatgga cctttacggt cgccatatac aagtagtgct gcgtaaaaaa atacgcaatg
     4141 agcagcgatt tgcgtcgctg gacgaactga aagcgcagat tgcgcgtgat gaattaaccg
     4201 cccgcgaatt ttttgggcta acaaaaccgg cttaagcctg ttatgtaatc aaaccgaaac
     4261 acggaaccga gaatctgatg agtgactata aatcaaccct gaatttgccg gaaacagggt
     4321 tcccgatgcg tggcgatctc gccaagcgcg aaccgggaat gctggcgcgt tggactgatg
     4381 atgatctgta cggcatcatc cgtgcggcta aaaaaggcaa aaaaaccttc attctgcatg
     4441 atggccctcc ttatgcgaat ggcagcattc atattggtca ctcggttaac aagattctga
     4501 aagacattat cgtgaagtcc aaagggcttt ccggttatga ctcgccgtat gtgcctggct
     4561 gggactgcca cggtctgccg atcgagctga aagtagagca agaatacggt aagccgggtg
     4621 agaaattcac cgccgccgag ttccgcgcca agtgccgcga atacgcggcg acccaggttg
     4681 acggtcaacg caaagacttt atccgtctgg gcgtgctggg cgactggtcg cacccgtacc
     4741 tgaccatgga cttcaaaact gaagccaaca tcatccgcgc gctgggcaaa atcatcggca
     4801 acggtcacct gcacaaaggc gcgaagccag ttcactggtg cgttgactgc cgttctgcgc
     4861 tggcggaagc ggaagttgag tattacgaca aaacttctcc gtccatcgac gttgcttttc
     4921 aggcggtcga tcaggatgca ctgaaagcaa aatttgccgt aagcaacgtt aacggcccaa
     4981 tctcgctggt gatctggacc actacgccgt ggactctgcc tgcgaaccgc gcaatctcta
     5041 ttgcacctga tttcgactat gcgctggtgc agatcgacgg tcaggccgtg attctggcga
     5101 aagatctggt tgaaagcgta atgcagcgta tcggcgtgac cgattacacc attctcggca
     5161 cggtaaaagg tgcggagctt gagttgctgc gctttaccca tccgtttatg ggcttcgacg
     5221 ttccggcaat cctcggcgat cacgttaccc tagatgccgg taccggtgcc gttcacaccg
     5281 cgcctggcca cggcccggac gactatgtga tcggtcagaa atacggcctg gaaaccgcta
     5341 acccggttgg cccggacggc acttatctgc cgggcactta tccgacgctg gatggcgtga
     5401 acgtcttcaa agcgaacgac atcgtcgttg cgctgctgca ggaaaaaggc gcgctgctgc
     5461 acgttgagaa aatgcagcac agctatccgt gctgctggcg tcacaaaacg ccgatcatct
     5521 tccgcgcgac gccgcagtgg ttcgtcagca tggatcagaa aggtctgcgt gcgcagtcac
     5581 tgaaagagat caaaggcgtg cagtggatcc cggactgggg ccaggcgcgt atcgagtcga
     5641 tggttgctaa ccgtcctgac tggtgtatct cccgtcagcg cacctggggc gtaccgatgt
     5701 cactgttcgt gcacaaagac acggaagagc tgcatccgcg tactctcgaa ctaatggaag
     5761 aagtggcaaa acgcgttgaa gttgacggca tccaggcgtg gtgggatctt gatgcgaaag
     5821 agatcctcgg cgatgaagct gatcagtacg tgaaagtgcc ggacacattg gatgtatggt
     5881 ttgactccgg atctacccac tcttctgttg ttgacgtgcg tccggaattt gccggtcacg
     5941 cagcggacat gtatctggaa ggttctgacc agcaccgtgg ttggttcatg tcttccctaa
     6001 tgatctccac cgcgatgaaa ggcaaagcgc cgtatcgtca ggtactgacc cacggcttta
     6061 ccgtggatgg tcagggtcgc aagatgtcta aatccatcgg caataccgtt tcgccgcagg
     6121 atgtgatgaa caaactgggc gcggatattc tgcgtctgtg ggtggcatca accgactaca
     6181 ccggtgaaat ggccgtttct gacgagatcc tgaaacgtgc tgccgatagc tatcgtcgta
     6241 tccgtaacac cgcgcgcttc ctgctggcaa acctgaacgg ttttgatcca gcaaaagata
     6301 tggtgaaacc ggaagagatg gtggtactgg atcgctgggc cgtaggttgt gcgaaagcgg
     6361 cacaggaaga catcctcaag gcgtacgaag catacgattt ccacgaagtg gtacagcgtc
     6421 tgatgcgctt ctgctccgtt gagatgggtt ccttctacct cgacatcatc aaagaccgtc
     6481 agtacaccgc caaagcggac agtgtggcgc gtcgtagctg ccagactgcg ctgtatcaca
     6541 tcgcagaagc gctggtgcgc tggatggcac caatcctctc cttcaccgct gatgaagtgt
     6601 ggggctacct gccgggcgaa cgtgaaaaat acgtcttcac cggtgagtgg tacgaaggcc
     6661 tgtttggcct ggcagacagt gaagcgatga acgatgcgtt ctgggacgag ctgttgaaag
     6721 tgcgtggcga agtgaacaaa gtcattgagc aagcgcgtgc cgacaagaaa gtgggtggct
     6781 cgctggaagc ggcagtaacc ttgtatgcag aaccggaact gtcggcgaaa ctgaccgcgc
     6841 tgggcgatga attacgattt gtcctgttga cctccggcgc taccgttgca gactataacg
     6901 acgcacctgc tgatgctcag cagagcgaag tactcaaagg gctgaaagtc gcgttgagta
     6961 aagccgaagg tgagaagtgc ccacgctgct ggcactacac ccaggatgtc ggcaaggtgg
     7021 cggaacacgc agaaatctgc ggccgctgtg tcagcaacgt cgccggtgac ggtgaaaaac
     7081 gtaagtttgc ctgatgagtc aatcgatctg ttcaacaggg ctacgctggc tgtggctggt
     7141 ggtagtcgtg ctgattatcg atctgggcag caaatacctg atcctccaga actttgctct
     7201 gggggatacg gtcccgctgt tcccgtcgct taatctgcat tatgcgcgta actatggcgc
     7261 ggcgtttagt ttccttgccg atagcggcgg ctggcagcgt tggttctttg ccggtattgc
     7321 gattggtatt agcgtgatcc tggcagtgat gatgtatcgc tcgaaggcca cgcagaagct
     7381 aaacaatatc gcttacgcgc tgattattgg cggcgcgctg ggcaacctgt tcgaccgcct
     7441 gtggcacggc ttcgttgtcg atatgatcga cttctacgtc ggcgactggc acttcgccac
     7501 cttcaacctt gccgatactg ccatctgtgt cggtgcggca ctgattgtgc tggaaggttt
     7561 tttgccttct agagcgaaaa aacaataata aaccctgccg gatgcgatgc tgacgcatct
     7621 tatccggcct acagattgct gcgaaatcgt aggccggata aggcgtttac gccgcatccg
     7681 gcaaaaatcc tcaaatataa gagcaaacct gcatgtctga atctgtacag agcaatagcg
     7741 ccgtcctggt gcacttcacg ctaaaactcg acgatggcac caccgccgag tctacccgca
     7801 acaacggtaa accggcgctg ttccgcctgg gtgatgcttc tctttctgaa gggctggagc
     7861 aacacctgtt ggggctgaaa gtgggcgata aaaccacctt ctcgttggag ccagatgcgg
     7921 cgtttggcgt gccgtcaccg gacctgattc agtacttttc ccgccgtgaa tttatggatg
     7981 caggcgagcc agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc
     8041 ctggcgtgat ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg
     8101 ccgggcagac cgttcatttt gatattgaag tgctggaaat cgatccggca ttggaggcgt
     8161 aacatgcaga tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc
     8221 agcattgttg aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg
     8281 gtacataacc gctatgtggt cgatagcttg cgtgagcgtg gggctatctt tattgagcag
     8341 attagcgaag taccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg
     8401 gtacgtaacg aagcaaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg
     8461 accaaagtgc atatggaagt cgcccgcgcc agccgtcgtg gcgaagagtc gattctcatc
     8521 ggccacgccg ggcatccgga agtggaaggt acaatgggcc agtacagtaa cccggaaggg
     8581 ggaatgtatc tggtcgaatc gccggacgat gtgtggaaac tgacggtcaa aaacgaagag
     8641 aagctctcct ttatgaccca gaccacgctg tcggtggatg acacgtctga tgtgatcgac
     8701 gcgctgcgta aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc
     8761 acgactaacc gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg
     8821 gtcggttcga aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc
     8881 aaacgcgcgt ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt
     8941 aaatgcgtcg gcgtgactgc gggcgcatcg gctccggata ttctggtgca aaatgtggtg
     9001 gcacgtttgc agcagctggg cggtggtgaa gccattccgc tggaaggccg tgaagaaaac
     9061 attgttttcg aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt
     9121 agcagcctaa gttatgcgaa aatgccggtc ttgttaccgg cattttttat ggagaaaaca
     9181 tgcgtttacc tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg
     9241 ccgcgatttt tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct
     9301 cggttgagaa aaccacccgc aacgccctgc aactgctgca tttctggaat gcggagattc
     9361 cgctcgccca gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc
     9421 acggcgaatc gggaatggct ggctacgact ttgttgagca caaccgaaag ccaatcggga
     9481 taccagcgtt tctggcgatt cgtgatgccc tgatgcgtgc accagagcct gttaccctgg
     9541 tggccattgg cccgttaacc aatattgcgc tgttactttc acaatgcccg gaatgtaaac
     9601 cgtatattcg tcgtctggtg atcatgggcg gttcttctgg tcgcggcaac tgtacaccaa
     9661 atgccgagtt taatatcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggaa
     9721 tcgaaatcgt catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc
     9781 tcgctacttt gccggagttg aatcgcaccg gaaaaatgct tcatgcactg tttagccact
     9841 accgtagcgg cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc
     9901 tggtgcgccc ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg
     9961 aatttacctc aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg
    10021 taaaggtggc attggatctg gatgtgaaag gcttccagca gtgggtggct gaggtgctgg
    10081 ctctggcgtc gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt
    10141 tcactgatat ccctccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg
    10201 cgtagcgatg cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct
    10261 atgcatgatg caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag
    10321 ttgattcagg cggcgctggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa
    10381 ggatcttctt tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt
    10441 accgtgcaaa gcagcctcga tgcggtaaaa gatgattttg atgtgtttat cgattttacc
    10501 cgtccggaag gtacgctgaa ccatctcgct ttttgtcgcc agcatggcaa agggatggtg
    10561 atcggcacta cggggtttga cgaagccggt aaacaggcga ttcgtgacgc cgccgccgat
    10621 attgcgattg tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg
    10681 gagaaagcag ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat
    10741 agacataaag ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac
    10801 gcccttgata aagatctgaa agattgcgcg gtctacagtc gtgaaggcca caccggtgaa
    10861 cgtgtgcctg gcaccattgg ttttgccacc gtgcgtgcag gtgacatcgt tggtgaacat
    10921 accgcgatgt ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgt
    10981 atgacatttg ctaacggcgc ggtaagatcg gctttgtggt tgagtggtaa ggaaaatggt
    11041 ctttttgata tgcgagatgt acttaatctc aataatttgt aaccacaaaa tatttgttat
    11101 ggtgcaaaaa taacacattt aatttattga ttataaaggg ctttaatttt tggccctttt
    11161 atttttagtg ttatgttttt aaatattcta taagtgccaa aaattacatg ttttgtcttc
    11221 tatttttgtt gttttaatgt aaattttgac catttggtcc actttttcct gctcgttttt
    11281 atttcatgca atcttcttgc tgcgcaagcg ttttccagaa caggttatat gatctttttg
    11341 tcgcttaatg cctgtaaaat atgcatgagc cacaaaataa tataaaaaat accgccatta
    11401 agttgacttt tagcgcccat atctccagaa tgccgccgtt tgccagaaat tcgtcggtaa
    11461 gcagatttgc attgatttac gtcatcattg tgaattaata tgcaaataaa gtgagtgaat
    11521 attctctgga gggtgttttg attaagtcag cgctattggt tctggaagac ggaacccagt
    11581 ttcacggtcg ggccataggg gcaacaggtt cggcggttgg ggaagtcgtt ttcaatactt
    11641 caatgaccgg ttatcaagaa atcctcactg atccttccta ttctcgtcaa atcgttactc
    11701 ttacttatcc ccatattggc aatgtcggca ctaatgacgc cgatgaagaa tcttctcagg
    11761 tacatgcaca aggtctggtg attcgcgatc tgccgctgat tgccagcaac ttccgtaata
    11821 ccgaagacct ctcttcttac ctgaagcgcc ataacatcgt ggcgattgcc gatatcgata
    11881 cccgtaagct gacgcgttta ctgcgcgaga aaggcgcaca gaatggctgc attatcgcag
    11941 gcgataaccc ggatgcggcg ttggcgttag aaaaagcccg cgcgttccca ggtctgaatg
    12001 gcatggatct ggcaaaagaa gtgaccaccg cagaagccta tagctggaca caagggagct
    12061 ggacgttgac cggcggcctg ccagaagcga aaaaagaaga cgagctgccg ttccacgtcg
    12121 tggcttatga ttttggtgcc aagcgcaaca tcctgcggat gctggtggat agaggctgtc
    12181 gcctgaccat cgttccggcg caaacttctg cggaagatgt gctgaaaatg aatccagacg
    12241 gcatcttcct ctccaacggt cctggcgacc cggccccgtg cgattacgcc attaccgcca
    12301 tccagaaatt cctcgaaacc gatattccgg tattcggcat ctgcctcggt catcagctgc
    12361 tggcgctggc gagcggtgcg aagactgtca aaatgaaatt tggtcaccac ggcggcaacc
    12421 atccggttaa agatgtggag aaaaacgtgg tgatgatcac cgcccagaac cacggttttg
    12481 cggtggatga agcaacatta cctgcaaacc tgcgtgtcac gcataaatcc ctgttcgacg
    12541 gtacgttaca gggcattcat cgcactgata aaccggcgtt cagcttccag ggtcacccgg
    12601 aagccagccc tgggccacac gacgcagcgc cgttgttcga ccactttatc gagttaattg
    12661 agcagtaccg taaaaccgct aagtaatcag gagtaaaaga gccatgccaa aacgtacaga
    12721 tataaaaagt atcctgattc tgggtgcggg cccgattgtt atcggtcagg cgtgtgagtt
    12781 tgactactct ggcgcgcaag catgtaaagc cctgcgcgaa gagggttacc gcgtcattct
    12841 ggtgaactcc aacccggcga ccatcatgac cgacccggaa atggccgatg cgacctacat
    12901 cgagccgatt cactgggaag tagtacgcaa gattattgaa aaagagcgcc cggacgcggt
    12961 gctgccaacg atgggcggtc agacggcgct gaactgcgcg ctggagctgg agcgtcaggg
    13021 cgtgttggaa gagttcggcg tgactatgat tggtgcgacc gccgatgcga ttgataaagc
    13081 agaagaccgc cgtcgtttcg acgtagcgat gaagaaaatt ggtctggaaa ccgcgcgttc
    13141 cggtatcgca cacacgatgg aagaagcgct ggcggttgcc gctgacgtgg gcttcccgtg
    13201 cattattcgc ccatccttta ccatgggcgg tagcggcggc ggtatcgctt ataaccgcga
    13261 agagtttgaa gaaatttgcg cccgcggtct ggatctctcc ccaaccaaag agctgctgat
    13321 tgatgagtcg ctgatcggct ggaaagagta cgagatggaa gtggtgcgtg ataaaaacga
    13381 caactgcatc atcgtctgct ctatcgaaaa ctttgatgcg atgggcatcc ataccggtga
    13441 ctccatcact gtcgcgccag cccaaacgct gaccgacaaa gaatatcaaa tcatgcgtaa
    13501 cgcctcgatg gcggtgctgc gtgaaatcgg cgttgaaacc ggtggttcca atgtccagtt
    13561 tgcggtgaac ccgaaaaacg gtcgcctgat tgttatcgaa atgaacccac gcgtgtcccg
    13621 ttcttcggcg ctggcgtcga aagcgaccgg tttcccgatt gctaaagtgg cggcgaaact
    13681 ggcggtgggt tacaccctcg acgaactgat gaacgacatc actggcggac gtactccggc
    13741 ctccttcgag ccgtccatcg actatgtggt tactaaaatt cctcgcttca acttcgaaaa
    13801 attcgctggt gctaacgacc gtctgaccac tcagatgaaa tcggttggcg aagtgatggc
    13861 gattggtcgc acgcagcagg aatccctgca aaaagcgctg cgcggcctgg aagtcggtgc
    13921 gactggattc gacccgaaag tgagcctcga tgacccggaa gcgttaacca aaatccgtcg
    13981 cgaactgaaa gatgctggcg cagagcgtat ctggtacatc gccgatgcct tccgtgcggg
    14041 cctgtctgtg gacggcgtgt tcaacctgac caatattgac cgctggttcc tggtacagat
    14101 tgaagaactg gtgcgtctgg aagagaaagt agcggaagtg ggcatcactg gcctgaacgc
    14161 tgaattcctg cgccagctga aacgcaaagg ctttgccgat gcgcgtctgg caaaactggc
    14221 gggcgtgcgc gaagcggaaa tccgcaagct gcgcgaccag tatgacctgc acccggttta
    14281 taagcgcgtg gatacctgtg cggcagagtt cgccaccgac accgcttaca tgtactccac
    14341 ttatgaagaa gagtgcgaag cgaatccgtc taccgaccgt gaaaaaatca tggttctcgg
    14401 cggcggcccg aaccgtatcg gtcagggtat cgaattcgac tactgctgtg tacacgcctc
    14461 gctggcgctg cgcgaagacg gttacgaaac cattatggtt aactgtaacc cggaaaccgt
    14521 ctccaccgac tacgacactt ccgatcgcct ctacttcgag ccggtaactc tggaagatgt
    14581 gctggaaatc gtgcgtattg agaagccgaa aggcgttatc gttcagtacg gcggtcagac
    14641 cccgctgaaa ctggcgcgcg cgctggaagc tgctggcgta ccggttatcg gcaccagccc
    14701 ggatgctatc gaccgtgcag aagaccgtga acgcttccag catgcggttg agcgtctgaa
    14761 actgaaacaa ccggcgaacg ccaccgttac cgctattgaa atggcggtag agaaggcgaa
    14821 agagattggc tacccactgg tggtacgtcc gtcttacgtt ctcggcggtc gggcgatgga
    14881 aatcgtctat gacgaagctg acctgcgtcg ctacttccag acggcggtca gcgtgtctaa
    14941 cgatgcgcca gtgttgctgg atcacttcct tgatgacgcg gtagaagttg acgtggatgc
    15001 catctgcgac ggcgaaatgg tgctgattgg cggcatcatg gagcacatcg agcaggcggg
    15061 cgtgcactcc ggtgactccg catgttctct gccagcctac accttaagtc aggaaattca
    15121 ggatgtgatg cgccagcagg tgcagaaact ggccttcgaa ttgcaggtgc gcggtctgat
    15181 gaacgtgcag tttgcggtga aaaacaacga agtctacctg attgaagtta acccgcgtgc
    15241 ggcgcgtacc gttccgttcg tctccaaagc caccggcgta ccgctggcaa aagtggcggc
    15301 gcgtgtgatg gctggcaaat cgctggctga gcagggcgta accaaagaag ttatcccgcc
    15361 gtactactcg gtgaaagaag tggtgctgcc gttcaataaa ttcccgggtg ttgacccgct
    15421 gttagggcca gaaatgcgct ccaccgggga agtgatgggc gtgggccgca ccttcgctga
    15481 agcgtttgcc aaagcgcagc tgggcagcaa ctccaccatg aagaaacacg gtcgtgcgtt
    15541 gctttccgtg cgcgaaggcg ataaagaacg cgtggtggac ctggcggcaa aactgctgaa
    15601 acagggcttc gagctggatg cgacccacgg cacggcgatt gtgctgggcg aagcgggcat
    15661 taatccgcgt ctggtgaaca aggtgcatga aggccgtccg cacattcagg accgcatcaa
    15721 gaatggcgaa tatacctaca tcatcaacac cacttcaggc cgtcgcgcga ttgaagactc
    15781 ccgcgtgatc cgtcgcagtg cgctgcaata taaagtgcat tatgacacca ccctgaacgg
    15841 tggtttcgct accgcgatgg cgctgaatgc cgacgcgacc gaaaaagtaa tttcggtgca
    15901 ggaaatgcac gcgcagatca aataatagcg tgtcatggcg gatatttttc atccgctaat
    15961 ttgatcgaat aagtaatacg gttctctgat gaggaccgtt ttttttgccc attaattaaa
    16021 tcttttgggg aatcgatatt tttgatgaca taagcaggat ttagctcaca
//
LOCUS       DAETQN010000098        15961 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.098, whole genome shotgun sequence.
ACCESSION   DAETQN010000098 DAETQN010000000
VERSION     DAETQN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.098"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="K0W68_003110"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="K0W68_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBI3074602.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="K0W68_003111"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="K0W68_003111"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBI3074603.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="K0W68_003112"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="K0W68_003112"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HBI3074604.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="K0W68_003113"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="K0W68_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="K0W68_003114"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="K0W68_003114"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBI3074605.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="K0W68_003115"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="K0W68_003115"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HBI3074606.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="K0W68_003116"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="K0W68_003116"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBI3074607.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="K0W68_003117"
     CDS             8229..8939
                     /locus_tag="K0W68_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBI3074608.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="K0W68_003118"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="K0W68_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBI3074609.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="K0W68_003119"
     CDS             9636..10343
                     /locus_tag="K0W68_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBI3074610.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="K0W68_003120"
     CDS             10343..10891
                     /locus_tag="K0W68_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBI3074611.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="K0W68_003121"
     CDS             10901..12067
                     /locus_tag="K0W68_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBI3074612.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="K0W68_003122"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="K0W68_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            13576..14229
                     /locus_tag="K0W68_003123"
     CDS             13576..14229
                     /locus_tag="K0W68_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBI3074613.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="K0W68_003124"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="K0W68_003124"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HBI3074614.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15961)
                     /locus_tag="K0W68_003125"
     CDS             complement(15612..>15961)
                     /locus_tag="K0W68_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBI3074615.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3700 a         4345 c         4162 g         3754 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttac catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaaac ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatagc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctggatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag ctcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccagccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 a
//
LOCUS       DAETQN010000099        15926 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.099, whole genome shotgun sequence.
ACCESSION   DAETQN010000099 DAETQN010000000
VERSION     DAETQN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15926
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.099"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..168
                     /locus_tag="K0W68_003126"
     CDS             <1..168
                     /locus_tag="K0W68_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3074616.1"
                     /translation="NRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFG
                     MTPGEYRRKFR"
     gene            complement(340..669)
                     /locus_tag="K0W68_003127"
     CDS             complement(340..669)
                     /locus_tag="K0W68_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBI3074617.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(674..859)
                     /locus_tag="K0W68_003128"
     CDS             complement(674..859)
                     /locus_tag="K0W68_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBI3074618.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(856..3495)
                     /locus_tag="K0W68_003129"
     CDS             complement(856..3495)
                     /locus_tag="K0W68_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBI3074619.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3703..4692
                     /gene="ldhA"
                     /locus_tag="K0W68_003130"
     CDS             3703..4692
                     /gene="ldhA"
                     /locus_tag="K0W68_003130"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBI3074620.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4803..5225
                     /gene="hslJ"
                     /locus_tag="K0W68_003131"
     CDS             4803..5225
                     /gene="hslJ"
                     /locus_tag="K0W68_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBI3074621.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5222..5488)
                     /locus_tag="K0W68_003132"
     CDS             complement(5222..5488)
                     /locus_tag="K0W68_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBI3074622.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5762..9286
                     /gene="nifJ"
                     /locus_tag="K0W68_003133"
     CDS             5762..9286
                     /gene="nifJ"
                     /locus_tag="K0W68_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBI3074623.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9652..10785
                     /gene="ompN"
                     /locus_tag="K0W68_003134"
     CDS             9652..10785
                     /gene="ompN"
                     /locus_tag="K0W68_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HBI3074624.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10926..11360
                     /gene="uspF"
                     /locus_tag="K0W68_003135"
     CDS             10926..11360
                     /gene="uspF"
                     /locus_tag="K0W68_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBI3074625.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11946..12860
                     /gene="sitA"
                     /locus_tag="K0W68_003136"
     CDS             11946..12860
                     /gene="sitA"
                     /locus_tag="K0W68_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBI3074626.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12860..13687
                     /gene="sitB"
                     /locus_tag="K0W68_003137"
     CDS             12860..13687
                     /gene="sitB"
                     /locus_tag="K0W68_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBI3074627.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            13684..14541
                     /gene="sitC"
                     /locus_tag="K0W68_003138"
     CDS             13684..14541
                     /gene="sitC"
                     /locus_tag="K0W68_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBI3074628.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14538..15395
                     /gene="sitD"
                     /locus_tag="K0W68_003139"
     CDS             14538..15395
                     /gene="sitD"
                     /locus_tag="K0W68_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBI3074629.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15596..>15926)
                     /locus_tag="K0W68_003140"
     CDS             complement(15596..>15926)
                     /locus_tag="K0W68_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBI3074630.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKQSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
BASE COUNT         3984 a         4022 c         3829 g         4091 t
ORIGIN      
        1 aaccgtcgtc tcgatttttg tgcagatgcg attcgccatg ccgcagatga tgaaaaactg
       61 gcaggcatcg gctttcattg gggattttct gaccagagtc atttttcaac ggtatttaag
      121 caacgctttg ggatgacgcc aggcgagtat cgacgtaaat tccgttaact aattcgcgtt
      181 gtgtgtactg caacgccaat tagttaaaac agatatttga ctatgcattt ttcagggcaa
      241 aaggcaattt gacaggagtt tttgaatatt caccttgttt caggaaaata gatcaatcgc
      301 tttctcctgt agcgttatcc cgatattgcg gttttgctat taaaattttc gcacccattt
      361 accgtttatc ccttgtacat attcccccgg tttggcccga gctacgagct tttgcccggc
      421 gagtttcgcg atatcatcta ccgacacatt attttgcttt gccagttgtt ggtaactcgc
      481 tttacgttcg gcattaatgt cggttactaa tttctctgtt tcagcatccg ttttcagcgc
      541 aaccagataa ccataaaatg tttcacctac ccgcccctga gttctggctt catccagagt
      601 caatgccatt acattgctgc ttaccagccc aacaagaaac gcacaaagaa gtaatgtttt
      661 cttcatcatc gcctcagaaa agatcgctac gagtttcaag cagctcttcg acatctttgt
      721 ctgccttgat gatgatctca tgctcaattt taacgttcat attgatagtg atcggttcct
      781 tgggcgcagc gacttcaata cgcggcgtac agccaaccag cataaaagat gacgtcaacg
      841 cagccagtaa aattttcatt gtttttcctc acactctttt ccttgcggac agtcatttcc
      901 cggcagacgt gcgttctgct ccaaccatgc ctggagatta tcgccaaagc gtaaactgcg
      961 ccacagcgta aaaatattct cttcatgatg gtaattaagg tttaccgtac cgcttttacc
     1021 atcaacgcga ctggtacctg taatgttggc ctgcatggtt aataaaccga gattatctaa
     1081 gttaattttt gtcgatgacc ggctaatttc catatagcgc aaccagttaa ttgctgaacc
     1141 cgcagtcata ttgtctttca ccaccgcatc tgcggtgtct ttatccagcc gcaaggtcat
     1201 tggcccgcta tttgccagcc aaccatcttt cactatccat ttttcgttgt ttagccataa
     1261 cggcaatgca ccactaaatg ccccggacat ggcgaattgt ttcggattga cggcgctaac
     1321 cagttcgctg gatgagagat tattcagccg caacagcgcc gggtcatgtt gcggcatacg
     1381 taattgtttc atcagtacgt taccgcctaa cacatcgacg ctaacatcag tcaacagcaa
     1441 gggttcttct tcagtccacg gataacgccc ttgcaaatca gccgtaatat ttttcgctgt
     1501 caccagatta atcacttcgg caattcgcaa cgtaacgggg ccgcgagtcc ccagatgcca
     1561 ggctccatcg gcaaaacgga aaggcaggac aaaatcgaca ccgttaacct gattatctgg
     1621 catccaggca ctaccgcctt tcaacacgcc gtgtcctccc gcgcggaatc cttgttcagg
     1681 cgcagcagaa aatgcaacct gagcatacag ttccccatcg cgtaagttca tcttccagtc
     1741 gggtggcacc agcggctgga atacagtcag tgattgtttt ggccaccagg cgttaccgcg
     1801 cagacgaata ccgtcccagc gaccatttac ccgaaccggg ccaatctcac cagcatgtaa
     1861 atcgccttta aagaggaaat acgtcggatc gcgcccatcg acgctaaatt ttaaggttga
     1921 tggcggtaac acactgccgc cagtgaacag cgtttgcccg gcgtccagtg acagcgcgcc
     1981 gctgaaggag ggatgctgtg cgtcacgtac ccagacgacg ggcttgtcga gaattaatcg
     2041 cggcttttct accgtcatcg taccgtattg taactggtcg aaaccggtgg agaggtcggt
     2101 cagcgtaatc gtgctgtcat gccattcacc ttttcctgcg acatcccagg tggcatttat
     2161 cggtgtaaaa ctgccttttc cccagtagcg ccactgccag cggcccgcgt cagggagaaa
     2221 atcattcgcc agcccatcca tatgcagcac gaaatcgccc agttcatatt catgcgcctg
     2281 caaaatggcc tgcaaacgtc cgtcaacacc acgttgggtg acttttacac ccgctaaagg
     2341 ccagcggatt tcatcgatgt ccagcgaatc gatgactctt cccttcgaac gtaataacgc
     2401 gcctggctca aaggccagcg ttgggtcagt cagacttcca cttagctgcg cgggtaagcg
     2461 ggcatataaa atgaggtccg cctgtttcgc ttcaccggtc agctgcagag gcagctggct
     2521 gttatccatg cttaattttc ctgggccaaa atttagcacc gcgttgcctt tacccgcttg
     2581 cccctgggtc agcacactca gtcgtccgct gatcagagcg ttctcaagcc ctgcctgcca
     2641 gttgtcgact ttgacaccca gtcggccact caaaggaaaa cctgcatacg gccaactcca
     2701 gcgaccatcg cttacggtca attgttgacg agtaatttgc cacggcaaat cgagcaacgg
     2761 atcgccgtta tcccgtgcca gcacaatcaa ttgcccgcta ttttcctgcc agtccagctc
     2821 tgcatccacc agtgacggtt cctgcggcaa gtttaacgtc gcagtagcat gcccacttac
     2881 aggaagtcca tccggcacga gcggcatagt aaattccccc accagtttaa ccggcggctg
     2941 attttcaaac gcgatgacat ccagttcgct gactgtaagt tgttgccctt tcagctggcc
     3001 ttgaaattta actttttcgc cctgataacg cagttgctga atatcagagg ttaatgcgag
     3061 agagagtttt ccctgccatt cctgccaggg agaaaaaatc agtttatcga tattgatcca
     3121 ggtgttaggc agcatggact gccactgcgc gagggttttt ggagcagctg gcgattgctc
     3181 cgtctgcggc aatttcgcca gacaagcaga atcaagttct accatgccga cgttcaataa
     3241 ccagcggctg ggatgtgaaa ggctggcgtt ggtgatatgc gcaagctgac aatctcccac
     3301 cagataacgg agatcgggaa tgattaaacc tttacgcgta atgcgtgggc tttcatctaa
     3361 tgcaatacgt gtcccgagcg gtagccagat gcccgccagc gtgggaaccc acagcccgag
     3421 cgtcatcagc agcgtcaacg gcacaagaat aatcagtaat aacagcgcga gaacggcttt
     3481 atatttaccc agcatgggta gttaatatcc tgatttagcg aaaaattaag cattcaatac
     3541 gggtattgtg gcatgtttaa ccgttcagtt gaaggttgcg cctacactaa gcattgttgt
     3601 tgatgaattt ttcaatatcg ccatagcttt caattatatt tgaaattttg taaaatattt
     3661 ttagtagctt aaatgtgatt caacatcact ggagaaagtc ttatgaaact cgccgtttat
     3721 agcacaaaac agtacgacaa gaagtacctg caacaggtga acgagtcctt tggctttgag
     3781 ctggaatttt ttgactttct gctgacggaa aaaaccgcta aaactgccaa tggctgcgaa
     3841 gcggtatgta ttttcgtaaa cgatgacggc agccgcccgg tgctggaaga gctgaaaaag
     3901 cacggcgtta aatatatcgc cctgcgctgt gccggtttca ataacgtcga ccttgacgcg
     3961 gcaaaagaac tggggctgaa agtagtccgt gttccagcct atgatccaga ggccgttgct
     4021 gaacacgcca tcggtatgat gatgacgctg aaccgccgta ttcaccgcgc gtatcagcgt
     4081 acccgtgacg ctaacttctc tctggaaggt ctgaccggct ttactatgta tggcaaaacg
     4141 gcaggcgtta tcggtaccgg taaaatcggt gtggcgatgc tgcgcattct gaaaggtttt
     4201 ggtatgcgtc tgctggcgtt cgatccgtat ccaagtgcag cggcgctgga actcggtgtg
     4261 gagtatgtcg atctgccaac cctgttctct gaatcagacg ttatctctct gcactgcccg
     4321 ctgacaccgg aaaactacca tctgttgaac gaagccgcct tcgatcagat gaaaaatggc
     4381 gtgatgatcg tcaataccag tcgcggtgca ttgattgatt ctcaggcagc aattgaagcg
     4441 ctgaaaaatc agaaaattgg gtcgttgggt atggacgtct atgagaacga acgcgatcta
     4501 ttctttgaag ataaatccaa cgacgtgatc caggatgacg tattccgtcg cctgtctgcc
     4561 tgccacaacg tgctgtttac cgggcaccag gcattcctga cagcagaagc tctgaccagt
     4621 atttctcaga ctacgctgca aaacttaagc aatctggaaa aaggcgaaac ctgcccgaac
     4681 gaactggttt aatcttgccg ctcccctgca ttccagggga gcttattcag ataatcccca
     4741 aagacctttc atcttctatt cttaaaatag ccctgagtca gaaactgtaa ttgagaacca
     4801 caatgaagaa agtagccgcg tttgttgcgc taagcctgct gatggcggga tgtgtaagta
     4861 atgacaaaat tgctgttacg ccagaacagc tacagcatca tcgctttgtg ctggaaagcg
     4921 taaacggtaa gcccgtgacc agcgataaaa atccgccaga aatcagcttt ggtgaaaaaa
     4981 tgatgatttc cggcagcatg tgtaaccgct ttagcggtga aggcaaactg tctaatggtg
     5041 aactgacagc caaagggctg gcaatgaccc gtatgatgtg cgctaacccg cagcttaatg
     5101 aactcgataa caccattagc gaaatgctga aagaaggtgc acaagtggat ctgaccgcga
     5161 accagttaac gctggcgacc gcgaaacaga cattaactta taagctggcg gatttaatga
     5221 attaatagct gccacagctc ccggcggcaa gtgactgttc gctacagcgt ttgccgttgg
     5281 gtaatgcaca catcccaatc gccgtaccat ccagttgacg ggcaacagaa agcgaaccgc
     5341 cgatcattgc acaatttgct tctccactac tggacatcga cgcttttaaa cctggcgcta
     5401 cgtgcgccgc agtcgcctgc tgaacaggtt cactactaca cgccgacaac aataaagcgg
     5461 cacaccctac ccaaaacgct gctcgcatct cttcttcctc tgatcttcaa gccaaacgac
     5521 accgccataa ataataggca gcacagaggg cggcgtcgag agctgtcctg cgcgttgccc
     5581 cgcattttta cttttttatg gctatttttt tgccctctgt ttgatcaaaa cattcattac
     5641 gctgatgtgg gggacacaaa agcgaaaatg cagaagaaag ccatttgcta aaattgaagg
     5701 attactactg ggcgcgcagc aatttcgtgc gcccctcatt tgcgcaatgt aagggtgtca
     5761 tatgattact attgatggta atggcgcggt tgcttcggtg gcgtttcgca ccagtgaagt
     5821 tatcgccatc taccctatta cccccagttc cacgatggca gaacaggctg atgcctgggc
     5881 cggaaacggc ttgaagaacg tttggggaga cacaccatgc gtggttgaaa tgcagtcgga
     5941 agcgggtgct atcgctaccg tgcatggcgc tttgcagacg ggtgcccttt caacatcgtt
     6001 tacgtcatcg cagggtttgc tgctgatgat cccgacactg tacaaactgg caggcgaact
     6061 cacaccgttt gtcctgcatg tagcggcacg taccgttgcc acacatgcac tctctatttt
     6121 tggcgatcat tccgacgtca tggcggtgcg ccagacgggt tgcgcgatgt tgtgtgcggc
     6181 aaacgtccag gaagcgcaag actttgctct catttcgcac atcgcgacgc tgaaaagccg
     6241 cgtgccattt attcatttct ttgatggttt ccgcacgtcc cacgaaatca acaaaattgt
     6301 cccgctggcc gatgacacga ttcttgatct catgccgcag gccgaaattg atgctcatcg
     6361 cgcccgggcg ctcaacccgg aacatccggt gatccgtggt acgtccgcca atcctgacac
     6421 ttatttccag tctcgcgaag ccaccaaccc ctggtacaac gcagtctatg accatgttga
     6481 acaggcgatg aatgatttct ctgccgcgac aggtcgtcag tatcagccgt ttgaatatta
     6541 cgggcatccg caagctgaac gggtgattat cctgatgggc tcagccattg gcacctgtga
     6601 agaagtggtt gatgaattgc taacccgtgg cgaaaaagtc ggcgtgctga aagttcgcct
     6661 gtaccgcccc ttctcagcca aacacttgct acaaactcta ccgggatccg tacgcagcgt
     6721 ggcggtactg gacagaacca aagaacccgg tgcccaggca gaaccgctct atctggatgt
     6781 gatgaccgca ctggcagaag cctttaataa tggcaagcgc gaaactctgc cccgtgtcat
     6841 tggtgggcgc tatggtcttt catccaaaga atttggcccg gactgtgtac tggcggtatt
     6901 tgccgagctc aacgcggcta aaccgaaagc gcgctttacg gttggtattt acgatgatgt
     6961 aaccaatctg tccctgccgt tgccggaaaa cacccagcca aactcggcga aacttgaagc
     7021 cttgttttat ggccttggta gtgatggcag cgtttccgcg accaaaaaca atatcaagat
     7081 tatcggtaat tccacgccgt ggtacgcaca gggctatttt gtttacgact ccaaaaaagc
     7141 gggcggcctg acggtttctc accttcgcgt gagcgaacag ccgattcgtt ccgcttatct
     7201 catttcccag gctgattttg ttggctgcca ccagttgcag tttatcgata aatatcagat
     7261 ggctgagcgt ttaaaacctg gcggcatttt cctgctcaac acgccgtaca gcgcagatga
     7321 agtgtggtcg cgcttgccgc aagaagttca ggccgtgtta aaccagaaaa aagcgcgctt
     7381 ctatgtgatt aacgcggcga aaatcgcccg caaatgtggc ctggcggccc gtattaatac
     7441 cgtcatgcag atggcttttt tccatctgac gcaaattctg cctggcgata gcgccctcgc
     7501 agaattgcag ggtgcgattg ccaaaagtta cagtagcaaa ggccaggatc tggtggaacg
     7561 caactggcag gctctggcgc tggcgcgtga atccgtagaa gaagttccgt tgcaaccggt
     7621 aaatccgcac agcgccaatc gaccgccagt ggtttccgat gccgcccctg atttcgtgaa
     7681 aaccgtaacc gcagcgatgc tcgcagggct tggtgatgcc ctccccgttt cggcgctgcc
     7741 gccagacggc acctggccga tgggcaccac tcgctgggaa aaacgcaata tcgccgaaga
     7801 gatccccatc tggaaagagg aactctgtac ccaatgtaac cactgcgtcg cagcttgccc
     7861 acactcagct attcgcgcaa aagtggtacc acccgaagcg atggaaaacg cccctgccag
     7921 cctgcattcg ctggatgtga aatcgcgtga tatgcgcggg cagaaatatg tcttgcaggt
     7981 ggctccggaa gattgcaccg gttgtaacct gtgcgtcgaa gtttgcccgg cgaaagaccg
     8041 tcagaatcca gagattaaag ccatcaatat gatgtcgcgc ctggaacatg tcgaagaaga
     8101 gaaaatcaat tacgatttct tcctcaacct gccagaaatc gaccgtagca aactggaacg
     8161 tattgatatt cgtacatcgc agctgattac accgctgttt gaatattccg gtgcttgctc
     8221 cggttgtggc gagacgccgt atattaaatt gctgactcag ctctatggcg accggatgtt
     8281 gatcgctaac gccaccggct gttcttcaat ttatggcggt aacctgccct ctacaccgta
     8341 taccaccgat gccaacggtc gtgggccggc atgggcgaac tctctatttg aagataatgc
     8401 cgaatttggc cttggtttcc gcctgacggt cgatcaacac cgtgtccgcg tgctgcgtct
     8461 gctggatcaa tttgccgata aaatcccggc ggaattactg acggcgttga aatcagacgc
     8521 tacgccagag gttcgtcgag aacaggtcgc agctttacgc cagcaactca acgatgttgc
     8581 cgaagcacat gaactgctac gtgatgcaga tgcactggtg gaaaaatcaa tctggctgat
     8641 tggtggtgat ggctgggctt acgatatcgg ctttggcggt ctggatcatg tattgagttt
     8701 gacggaaaac gtcaacattc tggtgctgga tacgcaatgc tattccaaca ccggtggtca
     8761 ggcgtcgaaa gcgacaccgc tgggtgcagt aactaaattt ggcgagcacg gcaaacgtaa
     8821 agcgcgtaaa gatcttggcg tcagtatgat gatgtacggt catgtttatg tggcgcagat
     8881 ttctctcggc gcgcagctga accagacggt gaaagcgatt caggaagcgg aagcgtatcc
     8941 gggaccatcg ctgatcattg cttatagccc gtgtgaagag catggttacg atctggcact
     9001 cagccatgac cagatgcgcc aactcacagc taccggcttc tggccgctat atcgcttcga
     9061 tcctcgtcgt gccgatgaag gcaaactgcc gctggcgctg gattcacgcc cgccgtcaga
     9121 agcactggaa gaaacgttac ttcacgagca acgtttccgt cgtctgaatt cgcagcagcc
     9181 agaagtggca gaacagttat ggaaagatgc tgcagctgat ttgcaaaaac gctatgactt
     9241 cctggcacaa atggccggaa aagcggaaaa aagcaacacc gattaaatgc tctggataag
     9301 gattacccaa ttctaaaaaa aagcccggac gactgttcgg gcttgtcttt ttatatgttg
     9361 gaaaatcagt ggcaatgcaa tggcccaaca gaaaataaaa tctgtgacaa taaaggcata
     9421 taacccgcgc agaataacgt atagaaaatt aaaagtataa tttttatttt gtatgtatta
     9481 tcttcgatac attcaccaga cttattatca tttcagaaga attatttgat taaggtttta
     9541 cttaaggcgt aacaaatgat atttatcggc taactgaact tctcctttag gatgttttca
     9601 ctccccggaa tggggataaa aggcaaataa aataactaaa ggatttattc aatgaaaagc
     9661 aaagtactgg cacttttaat tcctgccctg ctcgccgcag gtgctgcaca tgcagccgaa
     9721 gtttataata aagacggcaa caaattagat ctgtatggca aagttgatgg cctgcattat
     9781 ttttctgata attcagcgaa agatggcgat cagagctatg cgcgtctggg ttttaaaggc
     9841 gaaacccaaa ttaacgatca actcactggc tacggtcaat gggaatacaa tattcaggca
     9901 aacaacactg aatcttcaaa aaaccagtca tggacccgtc tggcatttgc cgggctgaaa
     9961 tttgcagatt acggttcttt cgattacgga cgtaattatg gcgtaatgta cgacatcgaa
    10021 ggctggaccg atatgctgcc tgaatttggc ggtgactctt ataccaatgc agacaacttt
    10081 atgactggtc gtgccaacgg cgtcgcgact tatcgtaata ctgatttctt cggtctggta
    10141 aatggtctga acttcgcggt gcagtatcaa ggtaacaacg aaggtgccag taatggtcag
    10201 gaaggcacca acaacggacg tgatgttcgc catgaaaatg gtgacggctg gggtctttcc
    10261 acaacatatg atttaggcat gggctttagc gctggtgcgg catacacctc atctgaccgc
    10321 accaatgacc aggttaacca tactgcggcg ggtggtgata aagcagacgc gtggactgct
    10381 gggctaaaat acgatgctaa caatatttac ctggcaacca tgtattcaga aacgcgtaat
    10441 atgaccccgt ttggcgacag cgattatgct gttgcaaaca aaacccagaa ctttgaagtc
    10501 actgcacagt accagtttga ttttggtctg cgtccggcag tctctttcct gatgtctaaa
    10561 ggccgtgacc tgcacgctgc cgatggtgca gacaacccgg caggtgttga tgataaagat
    10621 cttgttaaat acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacttat
    10681 gttgactata aaatcaacct gttggatgaa gatgacagtt tctacgctgc caacggcatc
    10741 tctaccgatg atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct
    10801 gttatggcgg gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact
    10861 ccgtcattac acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag
    10921 gttttatgaa cagaacgatt cttgtcccta tcgatatttc cgattcagaa ttaactcaac
    10981 gcgtgattag ccacgttgag gcagaggcaa agattgacga tgcagaggtt catttcctga
    11041 cggtaatacc ttcactgcct tactatgcct ctctgggttt agcgtattcc gcagaattac
    11101 cggcaatgga tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat
    11161 ttaaactgcc aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca
    11221 ttctggaatt ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg
    11281 atatcaccac ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct
    11341 ccgtgctggt tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt
    11401 tcgcaaatgt gctgacattt tcccctctaa tccgtaccat acacgccaca gttttatatc
    11461 agatttctta tgctgggcgt tccggcatga cgcatactct tctgatgcca tataacgaat
    11521 tgagtcgctt ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga
    11581 acaagtaagg gcaaaaatca caactatctg acccataagt attttattta tttttcaata
    11641 tgttaaacat tatcagcgac aacaataagc tacgataacc cactcttttt ttgccccttt
    11701 tttgcccctt ctacagcgtt ttgccccatt tttgccatca acaattttcc tcataaagtc
    11761 atttcaaagc ctaatagctg ctctactttt ttcctcaata tcatgtcgcc agcaggaaac
    11821 aacctaatcc ctgttttagg cctcaaagct gttaatgata atcattttca ttttgtatgg
    11881 gttatgctat aaaacatagc cgttgctatg tttgttctaa ttttgctcac tataggtact
    11941 aaattatgca ctcaataaaa aaagtaacca tgctcttggg gtgcctcgcc ctcacctgct
    12001 cgatcgcatt tcaggcaagt gcaactgaaa aattcaaggt cattacaaca tttaccatca
    12061 tcgcggatat ggccaaaaac gtggctggag atgctgcaga agtctcatcc ataaccaagc
    12121 ctggtgcaga aattcatgag tatcagccta cccctggcga tattaaacgt gcgcaggggg
    12181 cacaactgat tctcgccaat ggtatgaatc tggaattgtg gttccaacgc ttttaccagc
    12241 atctcaatgg ggttccagaa gtaattgtct cttcgggtgt gacgccagta gggatcaccg
    12301 aaggacccta tgagggcaaa cctaatcccc atgcatggat gtcgccagat aatgctctga
    12361 tttacgtcga taatattcgt gatgcgttga taaaatacga tccggcaaat gcacaaacct
    12421 accaacgtaa tgccgatact tataaagcca agattaccca aacccttgcc cccctgcgta
    12481 agcagattac ggaactccct gagaatcagc gatggatggt caccagtgaa ggggcttttt
    12541 cttatctcgc acgcgatttg gggctaaaag agctttatct gtggccgatt aatgccgatc
    12601 aacaaggaac accgcagcag gtacgtaagg ttgttgatat agttaagaaa aatcatatcc
    12661 cggcagtctt tagcgagagt acgatttccg ataaaccagc gcgtcaggtt gcgcgtgaaa
    12721 ccggcgcaca ctacggcggt gtgctctatg tcgattccct gagcacagaa aacggcccgg
    12781 tacctacgta tatcgacctt cttaaggtta ctaccagtac gctggtacag ggaattaaag
    12841 ccgggaaaag ggagaaataa tgatgcaatc tgcaggcatt gtcgttaatg atgttactgt
    12901 cacctggcgt aacgggcaca cagcactgcg cgatgcatcc ttcacagtac caagtggatc
    12961 tattgccgct ctggttgggg taaacggttc cggaaaatcg acgttgttta aagcgattat
    13021 gggatttgtg cgtctgacca gcggaaagat atctgttctg ggtattccca cgcgacaggc
    13081 gctacagaaa aacctggttg cctacgtccc tcagtcagaa gaggttgact ggtcatttcc
    13141 tgtactggtg gaagatgtgg tgatgatggg ccgctatggg catatgggta tgttgcgtat
    13201 agccaaaaag agggatcgtc agattgtcac cgatgcgctg gaacgtgtcg atatggtgga
    13261 ttttcgccat cgtcaaatcg gcgagctttc cggtggacaa aagaaacgcg tctttctggc
    13321 gagagcgatt gcacagcagg gtgatgtgat cctgctggat gaacctttta ccggcgttga
    13381 tgtgaaaacc gaagcgaaaa tcatcagcct gttgagggag ttacgcgcgg aaggcaaaac
    13441 gatgcttgtc tcaacgcaca atctcggctc agtcacgaca ttttgcgatt atacggtcat
    13501 ggtcaaaggc accgtcctgg caagcgggcc gacagacacc acttttacag ccgagaacct
    13561 ggagctggct tttagcggcg tactgcgcca tgtcacactt aacgactcgg aagaaagcat
    13621 tattactgac gatgaacgcc cttttgtggc acatcgaccg tcagcggtgc agagggaagc
    13681 aagatgaacg tgctactgga acccttcagc tatgaatata tgctcaatgc gatgtgggtc
    13741 tcggcaatgg tcggcggtct ctgcgctttc ctgtcatgct atctgatgct aaaaggctgg
    13801 tcgctgattg gcgatgcgct gtcgcactct attgtcccgg gtgttgcggg ggcatatatg
    13861 ctggggcttc cgttttctct gggagccttc ttttccggcg gactggcagc gggcagcatg
    13921 ctgtttctta accaacgcac ccgcttaaaa gaagatgcca tcatcggcct gatcttctcg
    13981 tccttttttg gcctggggct gtttatggtg tcattgaacc cgacatccgt gaacattcag
    14041 accatcgtcc tgggcaatat tctggcaatc gatccggcgg atatcctgca actcactatc
    14101 attggcattc tctcgatcat cgtgcttttt ttcaaatgga aagatcttat ggtgaccttt
    14161 ttcgacgaga atcacgcccg tgctattgga ttacatccgg gcagattaaa aatcctcttc
    14221 ttcaccctgc tgtcggtttc gaccgttgcg gcgctgcaaa cggttggtgc ctttctggtg
    14281 atctgtctgg tggtgactcc cggtgcaaca gcatggttgt taaccgatcg ttttccccgc
    14341 ctgctcataa ttgctgtaac tattggcagt gtaaccagtt tcctcggcgc gtgggtcagt
    14401 tactttctgg acggcgcgac aggtggaatc atcgtggtgg cgcagacact gctgtttctt
    14461 ttagcatttg tatttgcgcc cacccatgga cttttggcaa atcgtcggcg tgcgcataaa
    14521 gcgctggagg atcgctcatg atggcattat tactggagcc actgcagttc acctttatga
    14581 gtcacgcact gctgatttca ctggtagttt ccataccctg tgcgctgctg tcggtctttc
    14641 tggtcctgaa aggttgggca ctgatgggtg acgcgatgag tcatgcggtt tttcctggca
    14701 tcgtgcttac ctggattctg gggttacctc tggctacagg ggcattcgtc gccggagtat
    14761 tctgcgcggt ggcgacggga tacctgaaag acaacagccg aatcaagcaa gatacagtta
    14821 tggggattgt tttttctggc atgtttgccg caggtctgat cttgtatatt gcaatcaaac
    14881 cagacgtaca tcttgaccat attcttttcg gcgacatgct cgggataacc atcggcgata
    14941 taatccagac ggtgattatt gccgggctgg ttacacttgt tattagcgca aaatggcggg
    15001 attttttgct gttcagcttt gattatcaac aggcgcaggt aagcggtttg catacgagat
    15061 ggctgcatta tgggctgctg tgcatggttt ccctgaccat tgtggcgacg ctgaaagcag
    15121 taggtatcat tctctctatt tctctgctca ttgcgcctgg cgccattgca gtactcctga
    15181 cacaacgctt tcatattgcg ctcctactgg cgactggcat atcggtaata gtgtcaatga
    15241 ctggtgtctg gctgtccttt tttatcgaca gcgcaccagc tccgacgatt gtcgtcttat
    15301 tcgcggtcgt gttcatcatg acgtttaccg ttaccagcat caacgcacgc acaaagggaa
    15361 acgcccatac acaggatctg ttatcaccca attagcacaa catccgagga cagcatcgtc
    15421 cgccagccta cagaagtccg gaaagccatg gaaggtcgtt gggaggaggt acgaattcta
    15481 tcatgcaaaa aatacgtaaa atcgataacg cctagaaatc attcaatact cgcactatcg
    15541 aaagtttacc agccaaccgc ggcacgttcc tgcatacgac gtgccgcagc ttctgttatt
    15601 ccacgataaa attcctaacc gaattcaaat ccttcagact attcagctct tccttcagct
    15661 cccgctgacg gcgataaatc tcgtcattgc gctcaacctg tgcctgagcc attgctgccg
    15721 ccagttcttc cagttccggc atcgactgtt tcacctgctg attatcggca tcaccccacg
    15781 tcatcacgtc ccgtacaatg tcggatttcg cagccattac caccggataa aggcggccca
    15841 gtgagttggg gccagcattc cagatacgac cgttccattc aaacgtgaac ggcttcgcct
    15901 cctgttctgt gcgccatgct tcaatt
//
LOCUS       DAETQN010000100        15694 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.100, whole genome shotgun sequence.
ACCESSION   DAETQN010000100 DAETQN010000000
VERSION     DAETQN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15694
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.100"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..1453
                     /locus_tag="K0W68_003141"
                     /pseudo
     CDS             1..1453
                     /locus_tag="K0W68_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="K0W68_003142"
                     /gene_synonym="msbB"
     CDS             complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="K0W68_003142"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBI3074631.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="K0W68_003143"
     CDS             complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="K0W68_003143"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBI3074632.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="K0W68_003144"
     CDS             complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="K0W68_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBI3074633.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4981..5736
                     /gene="znuC"
                     /locus_tag="K0W68_003145"
     CDS             4981..5736
                     /gene="znuC"
                     /locus_tag="K0W68_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBI3074634.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5733..6518
                     /gene="znuB"
                     /locus_tag="K0W68_003146"
     CDS             5733..6518
                     /gene="znuB"
                     /locus_tag="K0W68_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBI3074635.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="K0W68_003147"
     CDS             complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="K0W68_003147"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBI3074636.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="K0W68_003148"
     CDS             complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="K0W68_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBI3074637.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="K0W68_003149"
     CDS             complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="K0W68_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HBI3074638.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8572..9174
                     /locus_tag="K0W68_003150"
     CDS             8572..9174
                     /locus_tag="K0W68_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBI3074639.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="K0W68_003151"
     CDS             complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="K0W68_003151"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBI3074640.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9732..10472)
                     /locus_tag="K0W68_003152"
     CDS             complement(9732..10472)
                     /locus_tag="K0W68_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI3074641.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="K0W68_003153"
     CDS             complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="K0W68_003153"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBI3074642.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="K0W68_003154"
     CDS             complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="K0W68_003154"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBI3074643.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            13153..13719
                     /locus_tag="K0W68_003155"
     CDS             13153..13719
                     /locus_tag="K0W68_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBI3074644.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            14074..14322
                     /locus_tag="K0W68_003156"
     CDS             14074..14322
                     /locus_tag="K0W68_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBI3074645.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            14923..>15694
                     /locus_tag="K0W68_003157"
     CDS             14923..>15694
                     /locus_tag="K0W68_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBI3074646.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSH"
BASE COUNT         3985 a         3974 c         3603 g         4132 t
ORIGIN      
        1 attttttata actattacta ataatatgac atcaattctt cgcagtgagt ttgatgaaat
       61 atggaaagcg atgctgacct tgcaattcca tgacatcctt cccggctcat gtatttcaag
      121 agtttatcat gaaactgaaa aggaatatct taaactagaa gcaaaaaccg agaaaattat
      181 ttctgatgcg cagtctactt tattaagtaa aattgacact tcttcttata aagatccgca
      241 cattttgttt aataccacct gttttgctcg taacgaatgg atcaatataa acaacaactg
      301 gctcaaagca cgagtaaact cttatggtta tgctgtgatt gttccaaaga ataagatcgt
      361 aaatggtctg aaggcagaat ctcaaagtat tgaaaataat tacattaaac ttcttttttc
      421 agaaagcggt gatttaattt cgctatatga taaaagatat ggtaaagaat acattactga
      481 aaatatgcac agtgagatcc gtgcatacca cgaagatgca ggttttttcg cggcctggga
      541 ttttgcttca aattatcgtg atggggaatc atatgtttta ctcgctgaaa aaatgacaac
      601 tgtgatcagc gggcctaaaa ctacgatgac gctgatttat cactacaatt cctcttatct
      661 tcgtttagcc tttaccctga cacaggatag tccacgcgtt gatgtccaaa cgtttattga
      721 ttggcacgaa cctaatgttt ctctcaaagt aaaattcccg gtgtcagtac aaacatctct
      781 tgcacaatgt caaattcagt tcggtgtaat tgacagacca acgcatagcg atgattcgtt
      841 cgcttttgcc aaagatgaaa taccagcaca tcactgggtc gacctttctg atcgagaaac
      901 tggcatagct ttaatagcaa aaaacaaata tggctatcgt gtcaaagacc agacgcttga
      961 attaactttg ctgcgttcgc agcataaacc tggggaagtt gttaagacag acaattatga
     1021 taatttcctg attaataatt ttgcagatat tggtaaacaa aaattcatat tctccttatt
     1081 tcctcatcat ggagattacc gtgtgggtgg tgttattaat caagcctata taatgaatca
     1141 tcccttacag gtcgtccctg tcaaaccgca cccgggtgaa ttaccccctt cactatcatt
     1201 ttttgctatc gatactaaca gcgttatcat cgaaacaata aaattagctg aagacggcga
     1261 cggtataatc gtaagattat atgagagtca tggattagaa atttcagcca tgctttcgtg
     1321 agtatgtaat tatcactacg ttcaatatgt cgatcttcaa gaaaataagt tagatgacag
     1381 tttttattgc aatcaatatt gcaatcttga gttcaaacct tttgaaatta tcacgctacg
     1441 attaactcaa tagcgtacta acctaaaaac tcaaaatagt gggaacactc attgagattt
     1501 tttatcgagt acagcctctc aataatttga gaggcttttt ttatttgata ggataaagat
     1561 ctttacgctt atacggttgg atttcgcccg gtttgcgagt tttcagcaat tttaatatcc
     1621 aggtgtattg ttctggtcgc ggaccaacaa aaatctcgac ttcttcattc atccgccgcg
     1681 caatcgtatg atcatccgcc tctaacagat catccatcgg tgggcgcacc tgaatcgtca
     1741 gacggtgcgt cttgccatca taaatcggaa acagcggaac aacgcgcgca cggcacactt
     1801 tcatcaaacg accaatcgcg ggcaacgtcg ctttataggt ggcaaagaaa tccacaaatt
     1861 cgctgtgctc tgggccatga tcctgatcgg gtaaataata tccccagtac ccctgacgta
     1921 ccgactggat gaatggttta ataccgtcat ttctcgcatg cagacgaccg ccaaagcgac
     1981 gacgcaccgt gttccagaca taatcaaaaa ccgggttgcc ctgattatgg aacatcgctg
     2041 ccattttctg cccttgcgag gccatcagca tggcaggaat atcgacggcc caaccgtgcg
     2101 gcaccagaaa gataactttc tcgttattac gcctcatctc ttcgatgatc tccagccctt
     2161 gccagtcaac gcgcggctga attttctccg gtccgcgtat tgccaactca gccatcattg
     2221 ccatcgcttg cggcgcggtg gcaaacatct catcaacaat cgcttcgcgt tcagcttcac
     2281 tacgttctgg aaagcagagc gacagattga ttaacgcacg acggcgtgag ctttttccca
     2341 gtcgtccggc aaaacgtccc agccgtgcca gaatgggatc acggaacttt ggcggcgtta
     2401 aagcgatacc cgccatcgct gctacaccca gccatgctcc ccagtagcgc gggtggcgaa
     2461 aggatttatc aaactcagga atgtattcgc tattattttt tttcgtttcc atgcttttcc
     2521 agtttcggat aaggcaaaaa tcaatctggt gatagtatag cggcgcaact tgccccgcac
     2581 caaataaaaa aagccgatac tgactgcgta ccggctgcga atggatgtta attaatcaaa
     2641 ccgtagctgc ggcacaatct ctttggcctg cgccaggaat tcgcgacgat cggagccggt
     2701 cagcccttcg gtacgcggca gttttgccgt cagcgggttt acggcctgct ggtttatcca
     2761 tacttcatag tgcagatgcg gcccggttga acgtccggta ttaccggaaa gcgcgatacg
     2821 gtcgccacgt ttcaccttct gtcccggttt caccagaatc ttgcgcaagt gcatataacg
     2881 cgtggtgtag ctgcgaccat gacgaatagc cacataataa cctgctgcgc cactgcgttt
     2941 ggcaaccacc acttcaccgt cacccactga aagcactggc gtaccctgcg gcatggcgaa
     3001 atcaacacct ctgtgtgctg caacgcgacc ggtcaccgga ttagtacgac gcgggttaaa
     3061 gttagacgag atacggaact gtttcgccgt cgggaatcgc aagaatcctt tcgccagacc
     3121 agtaccgtta cgatcgtaga atttgccatc ttcagcgcgg atagcgtaat aatctttacc
     3181 ttctgaacgc aaacgtacgc ccagcagctg gctttgctca cgtttaccat caagcatttc
     3241 tcgagacatt aacaccgcaa attcatcgcc ttttttcagt ttgcggaaat ccatttgcca
     3301 ctgcatggct ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt ttctggcgct
     3361 ggcaacaaag ctccccccga cggtaccttt cagcagattg ttaacccact ctccttgctg
     3421 catttcgctg gtcattttaa aaccgttagc ggcagtacgg tcataggttc gggtttcacg
     3481 acgagacact tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg tccaggagag
     3541 ttgttgaccg attttcaggt tacgcaattc tttgtcggcc gcagccagtt gggtgatatc
     3601 acccatatca ataccatact gattgagaat gctgcttagc gtatcgccag tggaaacaac
     3661 atattcatgc acgcccgctt caccggcgat tttgtcatcc agttcgtcct ggggaatggc
     3721 ttcatcttct tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga gcgaacgaat
     3781 ttcgttctgt tccagctcaa tggttttgac aattggcgtg gcatcgcggt gataaacata
     3841 gggccgccag acagcgacgg ccagagtaag aacggtgagc gaccccaaca taacgcggtg
     3901 tggtcgcggt aaattattaa acgccagggc gacagagcgg gctatctgtt gcacgtaatc
     3961 acttcctcat taatctcctt tcaggcagct cgcatactgg ttggctaatt gactcaggaa
     4021 ttctgaatag cttgttttac ccagtttgat attcgtcccc aggggatcca acgttcccat
     4081 acgaacggat gtccctcgtg cgacgctctc aacgaccgct ggcctgaact gtggctcagc
     4141 aaaaacgcag gttgcttttt gctcaaccaa ctgtgttctt atttcatgta aacgctgcgc
     4201 gccaggttga atctcagggt taacggtaaa atgaccaagc ggtgtcagtc cgaactgttt
     4261 ttcgaaatag ccgtaagcat cgtgaaaaac gaaataacct ttccccttga gcggcgcgag
     4321 ctcgttacca acctgcgttt cggttgaggc taattgtgcc tcaaaatcct tcaggttggc
     4381 gtcaagtttg gctcgacttt gcggcataag ttccactaat tttccatgga ttgcaaccgc
     4441 tgtagcccgc gctatctctg gggaaagcca aagatgcatg ttgaaatcgc cgtgatggtg
     4501 atcttcgtca cttttttccg cgtggtcgtg atcatcatca tcgccgtgaa tacttttcat
     4561 cagtagcggt ttcacatctt caagctgcgc aatcgttacc tgcttcgctc ctggtaattt
     4621 gcttaccggt ttttgcataa acgcttccat ctccgggcca acccaaacga ctaagtccgc
     4681 gttctgtaag cgttttacat ccgatgggcg cagtgaataa tcatgttctg aagcgccgtc
     4741 aggaagtaaa acctctgttt ctgttacccc atcagcaatg gcagaagcga tgaacccaac
     4801 gggtttaagc gaagcgacaa cggcggcatc tgcggcctgt gttgcacctc cccagagagc
     4861 ggcggataat gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac caatcatcgt
     4921 aatgaatatg agaagtgtga tattataaca tttcatgact actgcaagac taaaattaac
     4981 atgacaagtc tggtttcctt ggaaaatgtc tcggtttctt ttggccaacg ccgcgtcctc
     5041 tctgatgtgt cgctggaact taaacctgga aaaattttga ctttacttgg gccaaatggc
     5101 gcaggtaagt cgacactggt acgggtagtg ctcgggctgg taacacccga tgaaggggtt
     5161 atcaagcgca acggaaaact gcgcatcggc tatgtaccgc agaagctgta tctcgacacc
     5221 acgttgccac tgaccgtaaa ccgtttttta cgcttacgcc ctggtacaca taaagaagat
     5281 attttgcctg cactgaaacg tgtccaggcc gggcacctga ttaacgcacc gatgcaaaag
     5341 ctctctggtg gcgaaacgca gcgtgtactg ttagcgcgag cattgttaaa tcgcccgcaa
     5401 ttattagtgc tggatgaacc cactcaaggc gtggatgtaa atggccaggt ggcgttatat
     5461 gaccttattg accaactgcg tcgtgaactg gattgtggcg ttttaatggt ttctcacgat
     5521 ctgcatctgg tgatggcaaa aaccgatgaa gtgctgtgcc tgaatcacca catttgttgt
     5581 tccggcacac cggaagttgt ttccctgcat ccggagttta tttcaatgtt tggtcctcgt
     5641 ggtgctgaac aactgggtat ctatcgccat catcataacc atcgtcacga tttacaggga
     5701 cgaattgttt tgcgtcgggg aaatgatcgc tcatgattga attattattt cccggttggt
     5761 tagccgggat catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta gtctggcgtc
     5821 gtatgtctta tttcggtgat acgctggctc atgcctcatt acttggtgtc gcgtttggtt
     5881 tgttgctgga cgtgaatcca ttctatgcgg tgattgccgt tacgctgctg ctggcgggcg
     5941 gtctggtatg gctggagaag cgtccacagc tggcgatcga cacgttatta gggattatgg
     6001 cgcacagtgc cctgtcgctg ggcctggtgg tcgttagtct gatgtctaat attcgtgttg
     6061 atttgatggc ttacctgttc ggtgatttgc tggcagtgac gccagaagat ctcatctcta
     6121 ttgcgattgg cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc aatttgctgt
     6181 cgatgacgat tagcccggat ctggcgtttg ttgatggtgt gaaattacag cgcgtgaaat
     6241 tgttgttgat gctggtgacg gcattgacga ttggtgtagc gatgaaattt gtcggtgcgt
     6301 tgattattac ttcgttgctg attattcctg cagctactgc gcgtcgcttt gcccgtacgc
     6361 cggaacagat ggctggtgtc gctgttttgg tggggatggt ggcagtaact ggcggtttaa
     6421 ccttttccgc gttttacgat acgccggcgg gtccgtcggt agtgctatgt gctgcactgt
     6481 tatttattat cagtatgatg aaaaagcagg ccagctaatc tgtcgctgaa cacatttgtc
     6541 ggatgcggcg cgagcgcctt atccgaccta cggttcgcta tctctggtag gccggataag
     6601 acgcgaatag cgtcgcatca ggcacactgc caatgtcgga tgcggtgcga gcgaccaatc
     6661 cgacttacgg catttctggc ggcgttatgc caaagtgatt ccacgcccgc gtcgtcgcca
     6721 tacgcccacg cggtgtacgc tgcaaaaagc cttgctgaat cagataaggc tccagcacat
     6781 cctcaatggt ttcacgttct tcgccaatgg ctgccgccag gttatccaga cctaccggcc
     6841 caccaaagaa cttatcgatt accgccagca acaatttgcg gtccatataa tcgaaacctt
     6901 cagcatcgac attcaacata tccagcgcct gagcagcgat atctgccgag atggtgccat
     6961 cgtgcttcac ttcggcgaaa tcacgcactc gacgcagcag acggttggca atacgtggcg
     7021 taccgcgagc gcgacgagca acttccagcg cgccgtcatc actcatctca agccccataa
     7081 agcgtgcgct gcgactgacg atatattgca gatccggcac ctgataaaac tccagacgtt
     7141 gcacaatacc aaaacggtcg cgcaacggtg atgtcagcga acctgcgcgc gtggttgcac
     7201 caatcagggt aaacggcggc aaatcaattt taatggagcg tgccgccgga ccttcaccaa
     7261 tcatgatatc cagttggtag tcttccattg ccgggtacag cacttcttca acaactggcg
     7321 atagacggtg gatctcatca ataaacagca cgtcatgcgg ttcaaggtta gtgagcatcg
     7381 ctgccagatc gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa ttaacgccca
     7441 tttcattggc gacaatgttg gcaagcgtag ttttacccaa ccccggagga ccaaaaatca
     7501 acagatgatc gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgtg
     7561 aacgaacctg cggctgacca acatactctt ccagtaattt agggcgaatg gcgcgatctg
     7621 ccccatcttc cggcaaagtg gtaccggcag aaatcagacg gtctgcttca atcatccttt
     7681 acctcataac gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg cgtcagtgcg
     7741 agcgattttg ctcaccatgc ggcttgcttc ttgtggttta tagcccagcg ccaccagcgc
     7801 ggcaaccgct tcctgttcag catcgtcggt tgccgggctg gcaggcgacg tgagcaccag
     7861 atcggcggct ggcgtaaaga gatcgccgtg caaaccttta aatcggtctt tcatttcgac
     7921 aattaaacgc tcggcggttt ttttaccaat gcccggcagt ttcaccagtg ctcccacttc
     7981 ttcacgttca acggcattaa cgaactgctg cgctgacatt ccggagagga tcgccagcgc
     8041 caacttcggg ccgacgccgt tggttttgat taactcttta aacaacgtgc gctcttgttt
     8101 attgttaaaa ccgtacagca gttgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat
     8161 cgcttcctga cccgcttcag ggagttcata aaaacaggtc atcggcatat gcacttcata
     8221 gcctacgccg cccacttcaa ttaacaccag cgggggttgt ttttcaatga tgatgcctct
     8281 gagtctgcct atcacatgac gctcctgcgt aatgaatcaa agataatgct gtatgataaa
     8341 aaaatgctgg atagatatcc agcgaaggat gaagaaaact tgcgaggtgt ctcgatgaac
     8401 tgaaaaatgg cacagtataa tttattctac aggttatatt ggaagcaaat attttaatat
     8461 tacatattca gtgaataaat gcgtaataaa aatatacatt gcgcctcctg aaaaaaaata
     8521 aattttttat gctattacgt atattcgtat ctatttcaat ggaatgacaa cgtgaatatt
     8581 aattaccctg ctgaatatga aattggagat atcgtcttta catgtatagg tgctgcctta
     8641 tttggtcaaa tatcagctgc atcaaattgc tggagtaatc acgtcgggat cattatcggt
     8701 cataacggtg aagacttcct ggttgcagaa agccgggttc ccctttcaac catcaccacg
     8761 ctatctcgtt ttattaaacg ttcgtctaat caacgctatg ctataaagcg attagacgct
     8821 gggctgacag aacaacaaaa acaacgaatt gttgaacagg ttccctcccg gctacgcaaa
     8881 ctttaccaca ccggttttaa atacgaatct tcgcgccagt tctgttcaaa atttgttttt
     8941 gatatttata aagaagcgct atgcattccg gtgggtgaaa tagaaacgtt tggagaactg
     9001 ttaaatagca atccgaatgc aaaactcact ttctggaaat tctggttctt aggttctatt
     9061 ccgtgggagc gtaaaaccgt cacgccagcc agtttgtggc atcatccggg tctggtgttg
     9121 attcacgcgg agggagttga aacgcctcag cctgaactga ccgaggcggt ataacttaac
     9181 gcagtcgccc tctcgccagg ttcagccgcg attcgctcat ctgcattgca ttctggctga
     9241 cgtgacaatg ggtaatcgcg atagctaacg catcggcggc atccgcctgt ggattagcgg
     9301 gcagtttcag caaggtgcgg accatatgct gcacctggct tttttcggca ctaccaatac
     9361 ctaccaccgt ttgctttacc tgacgtgccg cgtattcaaa taccggcaat tcctgattca
     9421 ccgccgccac aatcgccacg ccgcgcgcct gccccagttt cagagccgag tcggcatttt
     9481 tggccataaa gacttgttca atggcgaaat aatctggctg gaactgggtg atgatttccg
     9541 tcacgcccgc atagatgagc ttcagacgag acggtaaatc atccactttg gtgcgtatgc
     9601 atccgctacc caggtaggac agttgcctgc ctacctggcg gatgacacca tagccgatca
     9661 cgcgcgaacc cgggtcaatg ccgagaataa tagccatcac gcgtctccgt tttgctgttt
     9721 agcaggcccc atcagagagt tgctgcaacc tcatcagaga tttcaccgtt atggtagact
     9781 tcctgcacgt cgtcgcaatc ttccagcata tcgatcagac gcatcagttt cggtgcggtt
     9841 tctgcatcca tatcagcttt ggtggacggg atcatggaaa cttccgcgct gtctgctttc
     9901 agacctgccg cttccagagc gtcgcgcact ttacccattt cttcccatgc agtgtagacg
     9961 tcaatcgcgc cgtcatcata ggtcacaacg tcttcagcac ctgcttccag tgctgcttcc
    10021 ataatggtgt cttcatcgcc tttctcgaag gagatcacgc cttttttgct gaacagatag
    10081 gcaacggaac catcagtacc gaggttaccg ccacatttgc taaatgcatg acgcacttca
    10141 gcaacggtac ggttgcggtt gtcagacaga cattcaatca tgattgccgt gccgccagga
    10201 ccgtaacctt cgtagatgat ggtttccatg tttgcatcat catcaccgcc cacaccacgt
    10261 gcaattgcgc ggttcagtgt gtcacgggtc atgttgttag acagtgcttt atccaccgcc
    10321 gcacgcagac gcgggttagc gtccggatcg ccaccgccca gcttagccgc ggttaccagc
    10381 tcacgaatga ttttagtgaa gattttaccg cgcttagcat cctgcgcagc tttacgatgt
    10441 ctggtgttgg cccatttact atgacctgcc ataaaaatat ctccagatag ccctgcctgt
    10501 tcaggcagcg ttaattacaa actgttcaat cgcctgccgg ttgctccagg acttagtgag
    10561 cgccgcggca gcaggcgcat caagccactt gtaagccaga tgttcagtga agactacctg
    10621 gcgttcgtgc gggagcgcaa gacagaacca tgattccgta ttacgcgtca cgcccggcgc
    10681 atagcgatga cgtaaatgtg aaaaaatttc aaactctacc gtgcgctgac agtcaattaa
    10741 ggtcagttgt tcagcgacaa catcaatggt gacctcttcc tttacttcgc gcatggcagc
    10801 ttgcggcgcg gtttcaccct cttccacgct gccggttacc gactgccaga aatcgggatc
    10861 gtcacgccgc tgcaacatca gcacccgttt cgtatcttgt gcatagatga ccactaagat
    10921 cgaaacggga cgcttataca ctttatcctt cacgctgcct cttcgttgac tgccttcgct
    10981 catcccattc acatagttat ctatgctcat gggagttcac tcagttgccg ccttgatgca
    11041 acgcgaatga tttcgtgtat ttgagtcata tcagttattc tcagccttct tcacaacctg
    11101 aatgctcagc tcagccagta cagtcgggtt agcaaagctc ggtgcttcag tcatcagaca
    11161 cgctgccgcc gtggttttcg ggaaggcgat aacgtcacgg atattgtcgg tgccagtcag
    11221 cagcatggtc agacggtcaa gaccgaatgc cagacctgcg tgcggcggag taccgtattt
    11281 cagggcgtcg agcaggaagc cgaatttctc gcgctgttcc tcttcgttga tacccagaat
    11341 accaaacacc gtctgctgca tatcaccatt atggatacgt actgaaccac cgcccacttc
    11401 gtaaccattg atgaccatat cgtaagcgtt cgccaccgca ttttccggtg cagctttcag
    11461 ttctgcagca gtcatgtctt tcggtgaggt gaacggatgg tgcattgccg tcaggccgcc
    11521 ttcaccgtcg tcttcaaaca tcgggaagtc gataacccac agcggtgccc atttgctttc
    11581 gtcggtcaga ccaaggtctt tacccacttt caggcgcagt gcgcccatcg cgtcggcaac
    11641 aattttcttg ttgtcggcac cgaagaaaat catatcgcca tcttgcgcgg cagtacgatc
    11701 caggatggct tcgatgattt ctgcattaag gaacttcgct accgggctgt tgataccttc
    11761 cagacctttc gcgcgttcgt taactttgat gtaagccaga cctttcgcgc cgtagatttt
    11821 aacgaagtta ccgtattcgt cgatctgctt acgggtcagc gatgcgccac ccggaacacg
    11881 tagagcggca acacggcctt tcggatcgtt cgccggcccc gcaaatactg caaactcaac
    11941 agatttcagc agatcggcaa cgtcggtcag ttccatcggg ttacgcagat ccggtttatc
    12001 agaaccataa cggcgttctg cttccgcaaa ggtcattacc gggaaatcgc ccagatccac
    12061 gcccttcact tccagccaca aatgacgcac cagcgcttcc atcacttcac gcacttgcgg
    12121 cgcggtcatg aaagaagttt ccacatcgat ctgagtaaat tcaggctgac gatcagcgcg
    12181 caggtcttcg tcacggaagc atttaacgat ctgatagtaa cggtcaaaac cggacatcat
    12241 cagcagctgt ttgaacaact gtggggattg cggcagtgcg tagaatttac ctttgtgcac
    12301 acgagaaggc accaagtagt cacgcgcgcc ttccggcgtg gctttggtca gcatcggagt
    12361 ttcgatgtcg aggaagccgt ggtcatccat aaaacggcgc accaggctgg tgattttagc
    12421 gcgggttttc aggcgctgcg ccatttccgg gcgacgcagg tcgaggtagc ggtatttcag
    12481 acgcgcttct tcggtgttga cgtggttaga gtcaagcggc agaacatctg cgcggttgat
    12541 gatagtcagc gaggacgcca gcacttcaat ttcgccggtc gccatatcgc ggttaatatt
    12601 tttttcgtca cgcgcacgta cggtgcccgt aacctgaatg cagaactcat tacgcagttc
    12661 agaggccagc tttaacgcgt ccgcacgatc cggatcgaaa aatacctgca cgataccttc
    12721 gcggtcgcgc atatcgatga agatcaggct accaagatca cgacgacggt tgacccaacc
    12781 acacagagtc acctgctgcc ccacgtggga caaacggagc tgtccacaat attctgtacg
    12841 catgagatat cccttaactt agctgccggc ggatgccccc tgctgcgcag gtgaccaagt
    12901 cgcagcgtta gctgtatgtc acaactgaat gaaaaaaggc ggctattata ctggaaattc
    12961 tgccgcaccg taagagcctg gcccgcgctg taacgcctcg ttaccacttt atatcgggcc
    13021 tgaaatcaga ctctacgcca gtttgctata aaggtgttgc ccgaactcat aaaaattaac
    13081 aaaatttgtc gttccgccat cggctaatcg cattaaggtg tgaggcacga ttttgttttt
    13141 tcaggagtca gcatgcttga acttaatgct aaaaccactg cgttggtggt gatcgattta
    13201 caagaaggca tcttaccttt tgccggtggt ccacatactg ccgatgaggt agttaatcgc
    13261 gccgggaagc tggcggcgaa atttcgcgcc agcggtcagc ccgtgtttct ggtgcgagtt
    13321 ggctggtctg ccgattacgc cgaagcatta aaacagccgg ttgatgcccc ctcccccgct
    13381 aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat taggtgcaac tgacagtgat
    13441 atcgaaatca tcaaacgtca gtggggtgcg ttttacggta cggatctgga gttgcaactg
    13501 cgccgccgag gtatcgatac aatagtgtta tgtgggatct cgaccaatat cggtgttgaa
    13561 tccaccgccc gcaatgcctg ggaacttggc tttaatctgg tgattgccga agacgcctgt
    13621 agtgccgcca gcgccgagca acacaataac agcattaatc atatctaccc gcgcatcgcc
    13681 cgtgtgcgta gcgtggaaga gatccttcac gcgttatgat ttacatcggt ctaccgcaat
    13741 ggtcgcatcc taaatgggtg cggttgggga tcaccagcct tgaagagtat gcccgccact
    13801 ttaactgcgt gatacgggat ttttaaaaat cactaaagaa cgcccaagag catgtgtttt
    13861 ctttagaata atcaaagcgt tggattgcca tgccatatga aaatcggcaa agttcatatg
    13921 taacaaagtg tcccaatcat gccccaaaat gccccacaac aaatattttt gccccatgca
    13981 tgccccaaaa aatcactttc cctcactctt gacactgttt atccatacag ttaaaaataa
    14041 tactgtataa aaacacagtg tagagggatt tttatgcgta ttgaaatctg catagccaaa
    14101 gagaaaatga ctaaaatgcc aaccggtgct gtggatgcgt taaaggaaga attaacccga
    14161 cgcatcagta aacgttatga cgatgtagag gtgatcgtaa aagccaccag caacgatggc
    14221 ctttctgtta cgcgcaccgc cgataaagat tcagctaaaa cttttgttca ggaaactctg
    14281 aaagatacct gggagtctgc tgacgagtgg tttgttcact aattagcacg taaaatcagt
    14341 aacggctgga aatcattcaa tactcgcact atcggaagtt caccagccag ccgcagcacg
    14401 ttcttgcata cgacgtgtct gcggtctcaa ctccgcccgc ccccgccaat catgattggg
    14461 cagtaccagt acagcactag caaagacagg aaattagcag tctcagcaaa acaccgacca
    14521 gacggtgaga agacaaaaag atacgcaaag atgccgcggt tccagaagaa taaaaaaccg
    14581 ctaatggaca gcctccttaa cacagttttt tatatacatg attcactgat accagtggca
    14641 ttaatagcta ttatatagct tcagctcctt ttttcatacc ttaaggaaaa attgccccct
    14701 cccccaaaaa gtatcgtttt tttacattaa accgatccag tttaggatag tgtaaagcag
    14761 aagttcttca atatataaca tatggagtta atatgcttac acaaactata tattcgcctc
    14821 ctccagaagt tcctgtctac tgctgaaggg aaccaataac attgcaaagc agataaaaaa
    14881 gaatccaaca cctctcaaca taaagaatac gccctgatat ttatgctccc gacaaataac
    14941 aatcacagat taatttcaaa ttcgttctcc acttattcaa tcgacactag ccgtgcatat
    15001 gaaaattatc taacccattg gactgaatgg aaaaataacc gcatacaaga agaacaacga
    15061 gacatcgctt ttcagcgact agtatcatgt ctacaaaacc aagagacgaa cctggacttg
    15121 tctgaattag gcctgacaac attacctgaa atccccccgg aaattaaatc aattaatata
    15181 agtaaaaata atttaagctt aatctcccca ttgcctgcgt cccttacaca gcttaatgtc
    15241 agctataaca gacttattga actgcctgct ttgcctcaag gacttaaatt attgaatgcg
    15301 tcccacaatc aactaatcac actacccaca ctccccatat ctttgaagga gcttcatgtc
    15361 tcaaataatc aattatgttc tcttcctgtt ttaccagaac tactggaaac attagatgta
    15421 tcatgtaatg ggctggcagt tttaccacct ttaccatttt ctttacaaga gattagcgca
    15481 atagggaatc ttcttagtga actcccccct ctacctcaca acattcactc catatgggca
    15541 atcgacaata tgttaaccga tattccatac ctgccggaaa atttaaggaa cggttatttt
    15601 gacataaatc agataagtca tatcccggaa agcattctta atctgaggaa tgaatgttca
    15661 atagatatta gtgataaccc attgtcatcc catg
//
LOCUS       DAETQN010000101        15584 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.101, whole genome shotgun sequence.
ACCESSION   DAETQN010000101 DAETQN010000000
VERSION     DAETQN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15584
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.101"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..2067
                     /locus_tag="K0W68_003158"
     CDS             <1..2067
                     /locus_tag="K0W68_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBI3074647.1"
                     /translation="SGYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNS
                     GLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSV
                     RFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITD
                     LQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHS
                     DFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGD
                     VFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYD
                     IADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLR
                     RISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFAS
                     NNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQA
                     GASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMT
                     PYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQP
                     LTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDES
                     RNYICQ"
     gene            2083..3117
                     /locus_tag="K0W68_003159"
     CDS             2083..3117
                     /locus_tag="K0W68_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HBI3074648.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3199..3537)
                     /gene="rcnB"
                     /locus_tag="K0W68_003160"
     CDS             complement(3199..3537)
                     /gene="rcnB"
                     /locus_tag="K0W68_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBI3074649.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3756..4580)
                     /gene="rcnA"
                     /locus_tag="K0W68_003161"
     CDS             complement(3756..4580)
                     /gene="rcnA"
                     /locus_tag="K0W68_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HBI3074650.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4701..4973
                     /gene="rcnR"
                     /locus_tag="K0W68_003162"
                     /pseudo
     CDS             4701..4973
                     /gene="rcnR"
                     /locus_tag="K0W68_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5065..5161
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5196..5984
                     /gene="thiM"
                     /locus_tag="K0W68_003163"
     CDS             5196..5984
                     /gene="thiM"
                     /locus_tag="K0W68_003163"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBI3074651.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5981..6781
                     /gene="thiD"
                     /locus_tag="K0W68_003164"
     CDS             5981..6781
                     /gene="thiD"
                     /locus_tag="K0W68_003164"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBI3074652.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6846..7664
                     /locus_tag="K0W68_003165"
     CDS             6846..7664
                     /locus_tag="K0W68_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBI3074653.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7716..8462
                     /locus_tag="K0W68_003166"
     CDS             7716..8462
                     /locus_tag="K0W68_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI3074654.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8436..9401)
                     /locus_tag="K0W68_003167"
     CDS             complement(8436..9401)
                     /locus_tag="K0W68_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBI3074655.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9398..10402)
                     /locus_tag="K0W68_003168"
     CDS             complement(9398..10402)
                     /locus_tag="K0W68_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBI3074656.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10399..11676)
                     /locus_tag="K0W68_003169"
     CDS             complement(10399..11676)
                     /locus_tag="K0W68_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3074657.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11933..12985
                     /gene="fbaB"
                     /locus_tag="K0W68_003170"
     CDS             11933..12985
                     /gene="fbaB"
                     /locus_tag="K0W68_003170"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBI3074658.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13304..14158
                     /gene="gatY"
                     /locus_tag="K0W68_003171"
     CDS             13304..14158
                     /gene="gatY"
                     /locus_tag="K0W68_003171"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBI3074659.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14187..15444
                     /gene="gatZ"
                     /locus_tag="K0W68_003172"
                     /pseudo
     CDS             14187..15444
                     /gene="gatZ"
                     /locus_tag="K0W68_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15454..>15584
                     /locus_tag="K0W68_003173"
     CDS             15454..>15584
                     /locus_tag="K0W68_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBI3074660.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         4089 a         3728 c         3988 g         3779 t
ORIGIN      
        1 agtggctatg ttccaccgga aaactgggag cggggtatta atgcgtttta tacctcttat
       61 tatgtgagtc agtattacag tgactataaa gcgtcgggta atagtaagag tacatatgta
      121 cgttttaaca gcgggttaaa tttactgggg tggcaactgc attctgatgc cagtttcagt
      181 aaaacaaata acaatccagg ggtgtggaaa agcaataccc tgtatctgga acgtggattt
      241 gcccaacttc tcggcacgct tcgcgtgggt gatatgtaca catcaagcga tatttttgat
      301 tctgttcgct tcagaggtgt gcggttgttt cgtgatatgc agatgttgcc taactcgaaa
      361 caaaatttta cgccacgggt gcaggggatt gctcagagta acgcgctggt aactattgaa
      421 cagaatggtt ttgtggttta tcagaaagag gttcctcctg gcccgtttgc gattacagat
      481 ttgcagttgg ccggtggtgg agcagatctt gatgtcagcg tgaaagaggc ggacggttcg
      541 gtaaccacct atctggtgcc ttatgcagcg gtgccaaata tgctgcaacc cggcgtgtcg
      601 aaatatgatt ttgcggcggg tcgtagccat attgaagggg cgagcaaaca tagtgatttt
      661 gtccaggcgg gttatcagta tggttttaat aacttattga cgttgtatgg tggctcgatg
      721 gttgcgaata attattacgc gttcactttg gggactggct ggaatacacg cattggtgcc
      781 atttccgtcg atgccacgaa gtcgcatagt aaacaagaca acggcgatgt gtttgacggg
      841 caaagttatc aaattgccta caacaaattt gtgagccaaa cgtcgacgcg ttttggtctg
      901 gcggcctggc gttattcgtc gcgtgattac cggacattta acgatcacgt ttgggcaaac
      961 aataaagata attatcgccg tgatgaaaac gatgtctatg acattgccga ttattaccag
     1021 aatgattttg gtcgcaaaaa tagcttttcc gccaatatga gccagtcatt gccagaaggt
     1081 tgggggtctg tgtcattaag tacgttatgg cgagattact gggggcgtag cggcagtagt
     1141 aaggattatc agttgagtta ttccaacaac ctgcgacgga taagctatac cctcgcggca
     1201 agccaggctt atgacgagaa tcatcatgaa gagaaacgtt ttaatatttt tatatcgatt
     1261 ccctttgatt ggggtgatga cgtttcgacg cctcgtcggc aaatatatat gtctaactca
     1321 acgacgtttg atgatcaggg gtttgcctca aataatacgg gattatcagg aacagtaggg
     1381 agtcgggatc agttcaatta tggtgtcaac ctgagtcatc aacatcaggg aaatgaaacg
     1441 acagctggag cgaatttaac ctggaacgcg ccggtggcaa cagtgaatgg cagttatagt
     1501 cagtcgagta cttatcgaca ggctggagcc agtgtttcag ggggcattgt cgcctggtcg
     1561 ggtggcgtta atctggcgaa ccgtctttcc gaaacgtttg ctgtgatgaa tgcgccagga
     1621 attaaagatg cttatgtcaa tgggcaaaaa tatcgcacaa caaaccgtaa tggagtggtg
     1681 gtatacgacg gaatgacacc ttatcgggaa aatcacctga tgctggatgt gtcgcaaagc
     1741 gatagcgaag cagaattacg tggcaaccgg aaaattgccg caccttatcg cggcgcggtt
     1801 gtactggtta attttgatac cgatcagcgc aagccctggt ttataaaagc gttaagagcg
     1861 gatgggcaac cattaacgtt tggttatgaa gtcaatgata tccacggtca taatattggc
     1921 gttgtcggcc agggaagcca gttatttatt cgcaccaatg aaataccgcc atcggttaat
     1981 gtagcaattg ataagcaaca aggactttca tgcacaatca ccttcggtaa agagattgat
     2041 gaaagtagaa attatatttg ccagtaatag tcaggtggtt ctatggaaat tcgcataatg
     2101 ctatttatat taatgatgat ggttatgcct gtgagctatg cggcatgtta tagtgagtta
     2161 tctgttcagc acaatttggt tgttcagggg gattttgcac ttactcaaac acaaatagcg
     2221 acatatgagc ataattttaa tgattcgtca tgcgtgagta caaatactat cacccctatg
     2281 agttcgtcgg atattattgt tgggctttat aacgatacca taaaattaaa tttacatttt
     2341 gaatggacca ataaaaacaa catcacgttg tcaaataatc agaccagttt caccagtggt
     2401 tattcagtta cggtgacacc tgcggccagt aatgcaaaag tgaatgtttc tgcggggggc
     2461 ggcggttcag tgatgattaa tggtgttgcg acattatcca gtgcttcatc atcgacacgc
     2521 gggagtgccg cagtacaatt tctactgtgt ttattaggtg gcaagtcatg ggatgcatgt
     2581 gtaaatagct acagaaatgc attggcacaa aatgcaggtg tctattcctt taatctgaca
     2641 ttgtcataca acccgataac cacaacctgc aaaccggacg atttattaat tactttagac
     2701 agtattcccg tttcacaatt accagccaca ggtaacaaag caacaataaa tagtaaacaa
     2761 ggggatatta ttctgcgttg taaaaattta ttaggtcaac aaaatcaaac atcacggaaa
     2821 atgcaggtgt atttatcaag ttctgatttg ttagccggca gtaacacgat cctgaaaggt
     2881 gcagaaaata atggagtcgg atttatgctt gaaagcaatg gttctccagt cacattttta
     2941 aatatcactg ataacagcaa aggatacaca aatttaaaag aaattgcggc aaagtcaaaa
     3001 cttacagata caacggtttc aattccgata actgccagtt actacgtcta cgatacaaac
     3061 aaagttaaat ctggcgcact ggaggcaacc gcattaatca acgtgaaata cgactaataa
     3121 caagcaagac gagcaagtgg ctaataataa aaaataacaa ggtgtctgca tttccccctc
     3181 atgatgaggg ggcttttttt agcgatgata aaaaatctca ccgtcgtagg ctttaatgat
     3241 tttaccgtcg gtgtcgctga tcaacacgta cgcgccaccc atataggtcc agtgcgtccc
     3301 ggcatcaggc gcgggcaggt tacgtaactg ccactgctta atgttgtact catcggtgcg
     3361 gtacatctct gggacggtat cacctggttt gaaatgctta aagtcagcaa tgaaactact
     3421 taactcatac ttagaaattc ccgaggtatt ggtcgaaccc gcggtggctg gtgcggccca
     3481 ggcggcactg gtaaccagca aaagcacacc cagcaacatc ttatttttaa tggtcataaa
     3541 ctctccactt tggggatctg taatatcagt cgcatatctt tcctgccata caatataact
     3601 gacaagcgat attttgttag tgatcccatg atttcgtaaa aagtgtaaat gaaaggatgc
     3661 cctaaaagca tccttagcga gcagagagtc agcggaaata gctgatttaa gaatcatcct
     3721 gggctgaaga taacatgcaa gcctaaagga ttgagttatc gcattatgcc catgaagcca
     3781 tgtacgccca tatacacacc gactaagcca atcaacagac tggaaaaata gggggcgcgt
     3841 ttagcgagag tgttaaatcc gctccagcgt tttgcgacct gctgaacgct gattgctgcg
     3901 ccaacgccta cggtgacaag cgttaacgcc aggccaatgc tgaaactgac gaccagtgtt
     3961 gcgcccagtg tcagggcttt caactgaatg catatcaaca gcacggtaat tgctgccggg
     4021 caggggataa ggccaccggt taagccaaat agcaaaattt gccagttggt gacctctcta
     4081 ccatcaaagc gtcgtttaat gtcattggca tgggctcgtg catgggcatc ctgatactcg
     4141 ccatgttcgt gatggtgatg atgtccatgg tcgtggtgat cttcgtgatc gtgatgatga
     4201 tgctcataat catactcgtg catattctcc agccagttgc gttcgccgcg ccaggtacgc
     4261 cagaacatcc agaacgcggt gccaataatg atcactgcgg aaatcagctg gagccacggt
     4321 tctgctgatt gagcagtaaa gcgcttgctg atcaccatcc cgccaaaggc aattaaccag
     4381 accactgcgg tatgcgaaat agttgctgcc agtccgagca tcaccgcttg tttaatggtg
     4441 cctttgatgg cgatgataaa cgccgccatc atcgtttttg agtgccctgg ttccaggcca
     4501 tgaagcgcac caagtaagat ggcgctgggg atgaagaacc aggcgtttcc ttgctgaaga
     4561 agagttgtaa attcggtcat gataataatt cttagtatta attcggcaat ctgattctac
     4621 tccccccagt acctgatact accccccagt agattaatag tgctatgata ttttcatatt
     4681 cttgttaacc aggtgttgcc atgtctcata caatccgtga taaacagaaa ctgaaagcgc
     4741 gtgccagtaa gattcagggc taggtcgtgg cgctcaagaa aatgctcgac gagccgcatg
     4801 aatgcgctgc agttttacaa cagattactg ctatccgtgg cgcggtaaac ggtctgatgc
     4861 gggaagtgat taaaggtcat ctgacggaac acatcgttca ccagggggat gagctaaaac
     4921 gtgaagaaga tctggatgtc gttctgaagg tgctggattc atatatcaaa taatttatta
     4981 acgcgattgt aaaactgccg tttttcctcg tttacaacgc gtgcgctgga cattaccatc
     5041 ctcctctgcg atttatcatc gcaaccaaac gactcggggt gcccttctgc gtgaaggctg
     5101 agaaataccc gtatcacctg atctggataa tgccagcgta gggaagtcac ggaccaccag
     5161 gtcattgctt cttcacgtta tggcaggagc aaactatgca agtcgacctg ctgggttcag
     5221 cgcaatctgc gcacgcgtta cacctttttc accaacattc ccctcttgtg cactgcatga
     5281 ccaatgatgt ggtgcaaacc tttaccgcta ataccttgct ggcgctcggt gcatcgccag
     5341 cgatggttat cgaaaccgaa gaggccagtc agtttgcggc tatcgccagt gccttgttga
     5401 ttaacgttgg cacactgacg cagccacgcg ctcaggcgat gcgtgctgcc gttgagcaag
     5461 caaaaagctc tcaaacaccc tggacgcttg atccagtagc ggttggtgcg ctcgattatc
     5521 gccgccattt ttgtcatgaa cttttatctt ttaaaccggc agcgatacgt ggtaatgctt
     5581 cggaaatcat ggcattagct ggcattgcta atggtggacg gggagtggat accactgacg
     5641 ccgcagctaa cgcgataccc gctgcacaaa cactggcacg ggaaactggc gcaatcgtcg
     5701 tggtcactgg cgaggtggat tatgttaccg atggccatcg cgccgttggc attcacggtg
     5761 gcgatccgtt aatgaccaaa gtggtaggaa ctggctgtgc attatcggcg gttgtcgctg
     5821 cctgctgtgc gttaccaggc gatacgctgg aaaatgtcgc atctgcctgt cactggatga
     5881 aacaagccgg agagcgcgca gtcgccagaa gcgaggggct aggcagtttt gttccacatt
     5941 tccttgatgc gctctggcaa ttgacgcagg aggtgcaggc atgaaacgaa ttaacgctct
     6001 gacgattgcc ggtactgatc cgagtggtgg tgcggggatt caggccgatc ttaaaacctt
     6061 ctcggcactt ggcgcttatg gttgctcagt tattactgca ctggtggcgc aaaatacccg
     6121 tggcgtacag tcggtgtatc gcattgagcc tgattttgtc gccgcccagc tcgattcggt
     6181 gttcagcgat gtgcgaatcg ataccactaa aatcggtatg ttggcggaaa ccgatattgt
     6241 tgaagcggtg gcagaacggt tgcaacgtta tcagatccaa aacgtggtac tcgacaccgt
     6301 tatgctggca aaaagcggcg acccactgct ttcaccttcg gcagttgcca cgctacgcag
     6361 tcgattattg ccacaggttt cattaataac gccaaacttg cccgaagcag ccgccttgct
     6421 cgacgcgcca cacgcgcgca ccgaacagga aatgctggaa caagggcgat cgctgttggc
     6481 gatgggctgt ggcgcagtgc taatgaaagg tggtcatctg gatgatgagc aaagcccgga
     6541 ctggctgttt acccgcgagg gtgaacaacg gtttaccgca ccgcgcatta tgaccaaaaa
     6601 cacccacggt actggttgta cactctctgc ggcgttggct gcactacgcc cgcgccatac
     6661 aaactgggct gacaccgtac aggaggcaaa aagctggctt tcatcggcgt tagcccaggc
     6721 cgacacgctg gaagttggtc acggtattgg tccggttcac cacttccacg cctggtggtg
     6781 agcaccgtac tatagctgct ttggttattg tcagcctttg gcactgacat tcagggatga
     6841 aaccaatgca gctaagaatt accagtcgaa aaaaactcac cgctctatta tgcgctcttg
     6901 ggctaatttc catcgttgca atttatccgc gtcagacggt gaattttttc tattcgacag
     6961 cagttcagat tacagactac atccacttct acggttatcg cccggttaaa tctttcgcta
     7021 ttcgtattcc tgccagttac accattcacg gaatagatgt ttcgcgctgg caggagcgga
     7081 tcgactggca gcgtgtggca aaaatgcgcg ataacggtat tcgcttacaa tttgccttta
     7141 ttaaggcgac ggaaggcgaa aagctggtgg acccctattt ttcgcgtaac tggcaactaa
     7201 gccgcgaaaa tggcttgctg cgcggggcat atcattattt ctccccgtcg gtagcagcac
     7261 cggttcaggt gcgattattt ctgcaaacgg tagatttctc acaaggcgat ttccctgcgg
     7321 tactggacgt agaagagcga ggaaaattat cggcaaaaga attacgcaag agggtaagtc
     7381 agtggctaaa aatggtcgaa aaaagtacgg ggaaaaagcc gattatttac tcaggagccg
     7441 ttttttatca cacgaatctg gcgggctatt tcaatgaata tccgtggtgg gtggctcact
     7501 actatcaacg tcgtccggac aatgacggca tggcctggcg cttctggcag cattccgacc
     7561 gtggacaggt agatggcatc aatggtccgg tggattttaa tgtgtttaat ggcacggtgg
     7621 aggagttgca gggattcgtt gatgggatta aagaaacgcc ttaaataaga agcagtgata
     7681 tactcccacc agactccctg gcaggaaaaa gacaaatgga acaagcgcat acccagctga
     7741 ttgcccaact gaacgaacgt attttagcgg cggataacac gccgctttat attaagtttg
     7801 ccgaaacggt aaaaaatgcc gtgcgcagcg gggtgctgga gcatggcaat attttgcccg
     7861 gtgagcgtga tttaagtcag ttaaccggcg tttcgcgcat tacggtgcgc aaggcgatgc
     7921 aggcgctgga agaagagggc gtggtgacgc gttcgcgcgg ttacggcacg cagatcaaca
     7981 acatcttcga atactcgttg aaagaagcgc gaggtttttc tcagcaggtg gtgttgcgcg
     8041 ggaaaaagcc cgatacgcta tgggttaaca agcgtgtcgt gaaatgtccc gaagaagtcg
     8101 cgcagcagct ggcggtcgaa gcaggaagtg atgtcttttt gcttaagcgt attcgctatg
     8161 tcgatgaaga agcggtatcg attgaggaat cgtgggttcc ggcgcattta attcatgatg
     8221 ttgatgccat cgggatttca ctttatgact atttccgcag ccagcacatt tacccacaac
     8281 gtacgcgttc ccgcgttagc gcccggatgc cggatgccga gtttcagtca catattcagt
     8341 tagatagcaa aataccggtg ctggtgatca agcaagtggc gcttgaccaa cagcaacggc
     8401 ctattgagta cagcattagc cactgtcgca gcgatctata cgtttttgtg tgcgaggagt
     8461 agttcctcgc gcgttggcgc acaatcaccg ccccgatgcc cgacaaccca cgacgccact
     8521 gcattgccca gtaatacggc atccgccagt ggcagaccag aggccagccc ggcaagtacg
     8581 ccacctgcat gactgtcgcc cgccccaatg gtgtctacaa cttgcgttgg aaatgccgga
     8641 atgcagccag aagcgtcgtt gctgaaatac catgcgcctt ctttatcgag acgaacgatc
     8701 aacggcgcgg caaatttctc ctgccattgc tcgccaagtg ttgttatctc tgcggataaa
     8761 gcaaaacgtt cggcggcaat ctcagcctct tgacgattga gcgacactaa aggtcgacag
     8821 gccatgatcc gcgccagtaa tgcatccggg atatcgccaa tacgtgggcc aaaatcgata
     8881 aacggcgtca cgtcttgcag ctcttccagc cattccacta acaattcgcc gcagggcgag
     8941 gccagttgat aaccggaaaa atagagcagg ctgccaggcg caacggttaa tcgcgccagc
     9001 cactggcgat tccactgatt ttccacaccg ctgaatgaca taaaagtgcg ttcgccatcc
     9061 ggctcaacca gcgccagaca ccagccgtta tcaccttcgg cgttatcgat caggctgatt
     9121 aagccctctt ttgccatccg gttgcgaata atctccgccc acacgccctg accgagcggc
     9181 aaggcattac ccgcttcgat gccgaggcgc tttaacgcca cggcaatatt cagtgcgcag
     9241 ccgccaacgt taacgctctg ctgtttcagt tcgatatcgc acccacgcca ggggagggca
     9301 taagcgtcgg cgatcacgtc aatgaccgcc gcgccgacga ccatcaccgg ctgacgcgtg
     9361 gttaattcag gcagcagcgt gtgtaatcga gcgccgctca taccgcctcc cgttgttgac
     9421 gatatttcgc cagcgctgtg gcatagcggt tgaaatcaag ctgatttacc gcatccagtt
     9481 ccgcctttaa cgcaggatcg atagcgttaa cgccatgcaa cgcgccgcaa attgccgtcg
     9541 ccatagcacc gatggtgtct gtgtcgccgc caaggttagc gcacaggacg gcgcagcgat
     9601 tcgggtcggt ttgtgctagt tcaaccagcg caatggcgca cggaacggac tcaatagtgc
     9661 tggtacctgc gccaacgacc tggtaaagct gttcgctggc ggattcggtg ccgtcggcat
     9721 tgcgcacaat tttcagcgca atctccagcc gcgccgccag tgaggcgctg aacgtagtga
     9781 tgcgtttttg ctgtgcatgt cgcgcaattg aaggcaggga atcaacaatc gctgaccagc
     9841 tttctccgtc aatggcacga gaaatcgccc atgcgatgac taccgcgcct gcaaccgcca
     9901 gatcggattt atgggtcggg ctggacgcca gcgctacatc atcaataaag gaatcaacat
     9961 cacgcgccgg aagcaaacaa cctaatggcg agacgcgcat cgctgcgccg ttggtcacgc
    10021 cgttgttttc cagttcagca acgggtttac cgtcgcgaat ggcgttaagc gcaatcttcg
    10081 aggtcggacc tagtacgttt ttgttaaagg cgtcgaaacg cagcgcccag tcgagaatat
    10141 tacgcccaat cagatccgga tcgatcttgc cttcacgttc cagtaacgca tccgccagac
    10201 acagcgccat cgaggtatcg tcggtgaatt cggcgcggtt aaaataacag gccgcgttat
    10261 tctcctttgg tccaggaaga aaacggtcaa tccagccaaa gtgtgcttta acgcggctgc
    10321 gtggccaaag ctcggagggc atccccatcg catcccctaa cgcctgccca taaagagcac
    10381 cgagaatacg ttctgttttc atttaacttc cccttgtgtc aacgcaatat cgcgatcatc
    10441 gaccttgata gccgtaattt cgttgtcgga ttcgcggaaa aaaatcatga acagcacggc
    10501 gataatggca atcatcaccg cgccgaaagt ccacatcccg gaccagttga aagtcagtcc
    10561 gtttaccggt tcctgataag cgaacatctt ttccatcatc acaccgccaa gacgatagcc
    10621 gagcaaactg ccgaagccct ggcagcagag cgtgatcagc ccctgcgcag cggtacgcat
    10681 atgcacgggg gcttttttat cgacatagat gtaagcggta acgtagtaaa aatcgtaact
    10741 tacgccgtga agcaaaatac cgaggaacag taacgcgtag gtgaaatatt catccgtact
    10801 accgtaaata aagaagccat agcggatcgc agcggtgacc agaccaagca ataatacctt
    10861 tttgatacca aagcgtttag tgaaaaacgg caatgccagc ataaagaaga tttcagagaa
    10921 ctggccgagc gtcatccagc cggtggcgtt tttcatgcca acttcggtca gataaccgtt
    10981 ggcaaagatg taatagaacg ccagtggcat cgcaaacagg aatgaacaga agaaaaagac
    11041 gaggaagttt ttatcgcgca gcaggatcag cgcatccagg ccgagcatga ctttaatatc
    11101 cattttgccg gtgctttttg gtggcgtgtc gggcaggaaa aacgcaaaca caccgagcag
    11161 agcagaactt ccggcggtaa tcagcagcgg gatgttagtc ggtgagatat cggcataccc
    11221 cagtatttgc ggcaagaaac cacatgccag accggaggca atccagccga tagtgcccat
    11281 cacacgaatg cgcgggaaat cacgctccac atccggcacg ttggcaaaag cgatgctgtt
    11341 agtcagcgca atggtcggca tataggttag agagtaagcc agcagtaacg gaaagaaccc
    11401 ggcaaaagtg gtctgttgcg cagcgaaata catcagcagc gcgcctgcga acatcaatac
    11461 cgccagcact ttttgcgccg agaaaaagcg gtcagtgatg gagccaacca gaattggcga
    11521 caggatcgcc gcaatggcgg tacaggcata cgaccagcca atttctccgg cactaaaacc
    11581 gcttttactt aaccacaacc acaatggcac aaaccacgcg ccccagataa accattcaac
    11641 aaacatcatg aacgacagct ttgctgttgt tttcatcttt taattccttc atgacaggta
    11701 atgtaaggta cgcgatgaca ataccactta acaatacctt ttaaatacct ttgcggtgaa
    11761 aacttgaccg cgctaacatt ttttctgatg aatcgagcca acagaaaacg ctgaaaaaac
    11821 atccaaaaga tggaaaaact cgcccgtaac gaccaggatt atgattgccc gcaaaaggcg
    11881 ggccaggaca atccatagcc gatatccaat cggaatttac gggagcatag taatgacaga
    11941 tattgcgcag ttgcttggca aagacgccga caacctttta cagcaccgtt gtatgacaat
    12001 tccttctgac cagctttatc tccccggaca tgactacgta gaccgcgtaa tgattgacaa
    12061 taatcgcccg ccagcggtgt tacgtaatat gcagacgttg tacaacaccg ggcgtctggc
    12121 tggcacagga tatctttcta ttctgccggt tgaccagggc gttgagcact ctgccggagc
    12181 ttcatttgct gctaacccgc tctactttga cccgaaaaac attgttgaac tggcgatcga
    12241 agcgggctgt aactgtgtgg cgtcaactta cggcgtgttg gcgtcggtat cgcggcgcta
    12301 tgcgcatcgc attccatttc tcgtcaaact taatcacaac gagacgctaa gttacccgaa
    12361 tacctacgat caaacgctgt atgccagcgt ggagcaggcc ttcaacatgg gcgcggtggc
    12421 ggttggtacg actatctatt ttggctcgga agagtcacgt cgccagattg aagaaatttc
    12481 tgcggccttt gaacgcgcgc acgagctggg catggtgacc gtgctgtggg cctatttgcg
    12541 aaactccgcc tttaagaaag atggcgttga ttaccatgtt tccgccgacc tgaccggtca
    12601 ggcaaaccat ctggcggcaa ccatcggtgc agatatcgtc aaacaaaaaa tggcggaaaa
    12661 taacggcggc tataaagcaa ttaattacgg ttacaccgac gatcgtgttt atagcaaatt
    12721 gaccagcgaa aacccgattg atctggtgcg ttatcagtta gctaactgct atatgggccg
    12781 ggctgggttg ataaactccg gcggtgctgc gggcggtgaa actgacctca gtgatgcagt
    12841 gcgtactgcg gttatcaaca aacgcgctgg cggaatgggg ctgattcttg gacgtaaagc
    12901 gttcaagaaa tcgatggctg acggcgtgaa actgattaac gccgtgcagg acgtttatct
    12961 cgatagcaaa attactattg cctgatacgc ctcatacgaa cagcaccacc ccgtaggacg
    13021 gataaggcgt ttacactgca tccggcaacc tatgcctgat gcgacgcgga agcgtcttat
    13081 catgcctaca tagcactgcc acgtatgtcg gaaaaggcgt ttacaccgca tccggcataa
    13141 aaacacgcgc actttgctac ggcttcccta tcgggaggcc gtttttttgc ctttcactcc
    13201 tcgaacagtt ttcatattgt cgttttgtga tcgttatctc gacatttaaa aacaaatcat
    13261 ttcattatat tttgaaatcg aaaacaagca acaggatatg aaaatgtacg tggtatcgac
    13321 aaagcagatg ctgaacaacg cacagcgcgg cggttatgcg gttccggcat tcaatattca
    13381 caatctcgaa acgatgcaag tggtggtgga aaccgctgcc aacctgcatg cgccggtcat
    13441 catcgccgga acgcctggca cattcactta tgctggtaca gaaaatctgt tggcgctggt
    13501 cagcgcgatg gcgaagcaat atcaccatcc actggcaatt catctcgacc atcacacgaa
    13561 atttgacgat atcgctcaga aggttcgttc tggcgtgcgc tcagtcatga ttgacgcctc
    13621 gcatttgcct tttgcgcaaa atatttcacg ggtcaaagag gtggtggatt tttgccatcg
    13681 ctttgatgtc agcgtcgaag cggagctggg gcaacttggc ggccaggaag atgatgtgca
    13741 agtcaatgaa gccgatgttt tttataccaa ccccgctcag gcgcgtgaat ttgccgaggc
    13801 aaccggaatt gattccctgg cggtcgccat cggcacggct catgggatgt atgccagcgc
    13861 accggcgctt gatttttctc gactggagaa cattcgccag tgggtgaact taccgctggt
    13921 gctgcatggc gcgtcagggt tatcgactaa ggatattcag caaaccatca aactggggat
    13981 atgcaaaatc aacgttgcaa cggagctgaa aaatgccttc tcgcaggcgt taaaaaatta
    14041 cctgaccgag caccctgaag cgaccgatcc ccgggattat ttgcagtcgg ctaaatccgc
    14101 aatgcgcgat gtggtgagca aagtgattgc cgattgtggc tgcgagggca gggcataacg
    14161 cacctgccat ttaacaagga aaaaacatga aaacgttaat tgcccggcat aaagctggtg
    14221 aacatatcgg catatgttca gtctgttctg cccatccgtt ggttatcgaa gcggcgctgg
    14281 catttgatcg caacagcacg cgcaaagtgc tgattgaagc aacgtcaaac caggtcaatc
    14341 aatttggcgg ttataccgga atgacaccgg cagactttcg cgaatttgtt tttacgattg
    14401 ccgataaagt cggatttgca cgcgaacgca ttattctcgg cggcgatcat ctggggccaa
    14461 actgctggca gcaagaaaat gcggatgcgg cgatggaaaa atccgtcgag ctggtaaaag
    14521 catatgttcg tgctggcttc agtaaaattc atcttgatgc gtcaatgtcc tgcgcggggg
    14581 atcccatacc gttagcacca gaaacggttg cggaacgagc tgctttgctt tgctgcggaa
    14641 agtgtggcga cagattgcca gcgtgagcaa ctgagctatg tcattggcac tgaagttccg
    14701 gttccgggcg gtgaggccag cgccattcag tcagtacaca tcacccatgt tgaagatgcc
    14761 gccaatactt tacgtacgca tcaaaaggcc tttattgccc gtgggctgac agaggcgtta
    14821 acacgcgtga ttgccatcgt ggtgcagccg ggcgtggaat ttgatcacag caatattatc
    14881 cattatcagc cgcaggaagc gcagccgctg gcgcaatgga tagaaaacac ccgaatggtt
    14941 tatgaagcac attctaccga ttaccagacc cggacggctt attgggaatt agtccgcgat
    15001 cactttgcaa tattgaaagt cggtcccgca ttaacctttg ctttacgcga ggcgatattt
    15061 gcactggcac aaattgagca ggaacttatc gcccctgaaa atcgcagcgg ttgcctggcg
    15121 gtaattgaag aagtgatgct cgacgaaccg caatactgga aaaaatatta tcgtacgggt
    15181 tttaacgatt cattactgga tattcgttac agcctgtcgg atcgtattcg ttattactgg
    15241 ccgcatagtc ggattaaaaa tagcgtcgaa acgatgatgg tgaatcttga aggcgtggac
    15301 atcccactgg gcatgattag tcagtatctt cccaaacaat ttgaacgcat tcagtccggg
    15361 gaattatcag caataccgca tcagctgatt atggataaaa tttatgatgt tttgcgcgcc
    15421 tatcgctacg gctgtgcgga ataaggacgg tatatgacta acctgtttgt tcgtagcgga
    15481 atttcttttg tcgatcgtag cgaagtttta acccatatcg gtaatgagat gctcgccaaa
    15541 ggtgtggttc atgatacctg gccacaggca ttaattgcca gaga
//
LOCUS       DAETQN010000102        15535 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.102, whole genome shotgun sequence.
ACCESSION   DAETQN010000102 DAETQN010000000
VERSION     DAETQN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15535
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.102"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1137
                     /locus_tag="K0W68_003174"
     CDS             <1..1137
                     /locus_tag="K0W68_003174"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBI3074661.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGSDIDMHIDAASGG
                     FLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYL
                     GGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYE
                     FICAGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMR
                     IMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1293..2828
                     /gene="gadC"
                     /locus_tag="K0W68_003175"
     CDS             1293..2828
                     /gene="gadC"
                     /locus_tag="K0W68_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBI3074662.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2959..4278
                     /locus_tag="K0W68_003176"
     CDS             2959..4278
                     /locus_tag="K0W68_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBI3074663.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4650..6032
                     /gene="dosC"
                     /locus_tag="K0W68_003177"
     CDS             4650..6032
                     /gene="dosC"
                     /locus_tag="K0W68_003177"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HBI3074664.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            6057..8456
                     /gene="dosP"
                     /locus_tag="K0W68_003178"
                     /gene_synonym="pdeO"
     CDS             6057..8456
                     /gene="dosP"
                     /locus_tag="K0W68_003178"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HBI3074665.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8715..9296
                     /gene="ddpX"
                     /locus_tag="K0W68_003179"
     CDS             8715..9296
                     /gene="ddpX"
                     /locus_tag="K0W68_003179"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HBI3074666.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            9310..10860
                     /locus_tag="K0W68_003180"
     CDS             9310..10860
                     /locus_tag="K0W68_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBI3074667.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10862..11884
                     /locus_tag="K0W68_003181"
     CDS             10862..11884
                     /locus_tag="K0W68_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3074668.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11881..12777
                     /locus_tag="K0W68_003182"
     CDS             11881..12777
                     /locus_tag="K0W68_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HBI3074669.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12774..13760
                     /locus_tag="K0W68_003183"
     CDS             12774..13760
                     /locus_tag="K0W68_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3074670.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            13789..14679
                     /locus_tag="K0W68_003184"
     CDS             13789..14679
                     /locus_tag="K0W68_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3074671.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(14735..15166)
                     /gene="osmC"
                     /locus_tag="K0W68_003185"
     CDS             complement(14735..15166)
                     /gene="osmC"
                     /locus_tag="K0W68_003185"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBI3074672.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3816 a         3875 c         3953 g         3891 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgc gacgaaaata ccatcggcgt ggtgccgact
      361 ttcggcgtga cctacaccgg taactatgag ttcccgcaac cgctgcacga tgcactggat
      421 aaattccagg ccgacaccgg tagcgacatc gacatgcaca tcgacgccgc cagcggtggc
      481 ttcctggcac cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa
      541 tcgatcagtg cttcaggcca caaattcggt ctggctccgc tgggctgcgg ctgggttatc
      601 tggcgtgacg aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctcggtggt
      661 cagattggta cttttgccat caacttctcc cgcccggcgg gtcaggtgat tgcacagtac
      721 tatgaattcc tgcgccttgg tcgtgaaggc tataccaaag tacagaatgc ttcctaccag
      781 gtcgctgcct atctggcgga tgagatcgcc aaactggggc cgtatgagtt catctgtgcc
      841 ggtcgcccgg acgaaggcat cccggcggtt tgcttcaaac tgaaagaggg tgaagatccg
      901 ggatacaccc tgtacgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc
      961 ttcactctcg gcggtgaagc caccgacatc gtggtgatgc gcattatgtg tcgtcgcggc
     1021 ttcgaaatgg actttgctga actgttgctg gaagactaca aagcctccct gaaatatctc
     1081 agcgatcacc cgaaactgca gggtattgcc cagcagaaca gctttaaaca tacctgataa
     1141 cgtttaacgg taacggtgtc ccgaaacgaa cccgtttcgg gataatttcc aaagtctgtt
     1201 cactggcatt agcaacggaa aatattgttc tgaatacgct tcagaacaaa acaggtgcgg
     1261 ttccgacagg aataccgttt tagggggata atatggctac atcagtacag acaggtaaag
     1321 ctaagcagct cacattactc ggcttttttg ccataacggc atcgatggta atggctgttt
     1381 atgaataccc taccttcgca acatcgggct tttcattagt cttcttcctg ctattaggcg
     1441 ggattttatg gtttattccc gtgggacttt gtgctgcgga aatggccacc gtcgacggct
     1501 gggaagaagg tggtgtcttc gcctgggtat caaatactct ggggccgaga tggggatttg
     1561 cagcgatctc atttggctat ctgcaaatcg ccattggttt tattccgatg ctctatttcg
     1621 tgttaggggc actctcctac atcctgaaat ggccagcgct gaatgaagac cccattacca
     1681 aaactattgc agcactcatc attctttggg cgctggcatt aacgcagttt ggtggcacga
     1741 aatacacggc acgaattgct aaagttggct tcttcgccgg tatcctgtta cctgcattta
     1801 ttttgatcgc attagcggct atttatctgc actccggtgc ccccgttgct atcgaaatgg
     1861 attcaaagac cttcttccct gacttctcta aagtgggcac cctggttgta tttgttgcct
     1921 tcattttgag ttatatgggc gtagaagcat ccgcaaccca cgtcaatgaa atgagcaacc
     1981 cagggcgcga ctatccgttg gctatgttac tgctgatggt agcggcaatc tgcttaagct
     2041 ctgttggtgg tttgtctatt gcgatggtca ttccgggtaa tgaaatcaac ctctccgcag
     2101 gggtaatgca aacctttacc gttctgatgt cccatgtggc accggaaatt gagtggacgg
     2161 ttcgcgtgat ctccgcactg ctgttgctgg gtgttctggc ggaaatcgcc tcctggattg
     2221 ttggtccttc tcgcgggatg tatgtcacag cgcagaaaaa cctgctgcca gcggcattcg
     2281 ctaaaatgaa caaaaatggc gtaccggtaa cgctggtcat ttcacagctg gtgattactt
     2341 ctatcgcgtt gatcatcctc accaataccg gtggcggtaa caacatgtcc ttcctgatcg
     2401 cactggcgct gacggtggtg atttatctgt gtgcttattt catgctgttt attggctaca
     2461 ttgtgttggt tcttaaacat cctgacttaa aacgcacatt taatatccct ggtggtaaag
     2521 gggtgaaact ggtcgtggct attgtcggtc tgctgacttc aattatggcg tttattgttt
     2581 ccttcctgcc gccggataac atccagggtg actctaccga tatgtatgtt gaattactgg
     2641 tcgttagttt cctggtggta cttgccctgc cctttattct ctatgctgtt catgatcgta
     2701 aaggcaaagc gaataccggc gtcactctgg agccaatcaa cagtcagaac gcaccaaaag
     2761 gtcacttctt cctgcacccg cgtgcacgtt caccacacta tattgtgatg aatgacaaga
     2821 aacactaacc atgtctgaat ggttataaga caagggagcg ataattcatc gctccctttt
     2881 tcgtgctcgc tgtcgtattg acttaaccgg gataagtacg agaatgagcg cacatctgtt
     2941 taccggaaac cagcatatat ggatatctgc tcccgaaacg agaaattagc gattagaaga
     3001 ccagcgatac tggttgcact ggcactttta ctgtgtagtt gtaaaagcac gcctccagag
     3061 tccatggtga caccaccagc aggttcaaag ccaccagcca cgacgcaaca atcgtcacaa
     3121 ccgatgcgtg gcatctggct ggccacggtg tctcgcctcg actggccacc ggtttcctcg
     3181 gttaacatta gtaaccccac cagccgggcc cgtgtacaac aacaggcgat gatcgacaaa
     3241 ctggatcatc tgcaacgtct cggtataaac acggtctttt tccaggttaa gccggacggt
     3301 accgccctgt ggccatcgaa aattttgcca tggtccgatc ttatgaccgg taagattggt
     3361 gaaaatccgg gttacgatcc gctgcaattt atgctcgacg aagcccacaa gcgtgggatg
     3421 aaagtacacg cctggtttaa cccctatcgc gtatcggtta atacgaagcc cggtactatc
     3481 agggaactga atagcactct gtctcaacaa ccggcgagcg tctatgtgca acaccgtgac
     3541 tggatcagaa cgtctggcga tcgctttgtc ctcgacccgg gcatccctga ggttcaggac
     3601 tggatcacat caatagtcgc agaagtggtt tcccgctatc cggtagatgg cgtgcagttt
     3661 gacgactatt tctatactga gtcaccgggt tcacggctaa atgataacga aacgtaccgc
     3721 aaatacggag gcgcatttgc gtcaaaagca gactggcggc gcaacaatac tcagcagtta
     3781 attgcaaaag tatcgcacac cattaaaagc attaagccgg gagtcgaatt tggcgttagc
     3841 ccggcaggcg tgtggcgtaa ccgatcacac gatccgctcg gttccgatac ccgaggcgcg
     3901 gcagcctatg acgaatccta cgctgacacc cgtcgatggg tggaacaagg attgctggat
     3961 tacattgctc cccaaattta ctggccgttc tcacggagtg ccgcgcgtta tgacgtgttg
     4021 gcaaaatggt gggcggatgt cgttaaaccg actaggaccc gcctgtatat cggtatcgcc
     4081 ttctataaag tgggtgaacc ttcaaagata gagccagact ggatgattaa cggcggcgta
     4141 ccggaactga aaaagcagct cgatcttaac gatgcggtac cagaaattag cggcaccatc
     4201 ttgttccgtg aggactatct gaataaaccg cagactcaac aagcggtcag ctatctgcaa
     4261 agtcgttggg gcagttaaac tcccatgctt actttgtaac aagccggtgc tttacccacc
     4321 ggcttgttac accttgtgaa atatcccact agtaatcatg cttacataag tcaaattaac
     4381 cacgcaatta accacactta gttaaacagc tataaatccc taataaaaat gtatgaatat
     4441 gaaaaaccat acgatattcg ctgtcaccga aaaataatta cgatgggcta tactcttctg
     4501 aagaacagta gcgtagattg aaactgtcct aagtcgctta tttcttttca gtatatcttc
     4561 atatttcagg agaatatggt gcagttagtc aatctcgcac gttgcgtttt gattccgtct
     4621 gatttcccag ccttataagg gtgtgaatta tggagatgta ttttaaaaga atgaaagatg
     4681 agtggaccgg acttgtcgaa caggcagatc cactcattcg tgctaaagcc gcggaaattg
     4741 ccgttgcgca tgctcattat ctgagtatcg agttttatcg aattgtccgc atcgacccgc
     4801 atgccgaaga attcttgagt aatgaacaag ttgagcggca gttgaaaagt gcgatggaac
     4861 gctggattat taacgtgctt tctgcccagg ttgacgatgt cgaaaggcta atacaaatcc
     4921 agcataccgt cgcggaagtg catgcccgca taggaattcc ggtagaaatt gtcgagatgg
     4981 ggtttcgggt gctgaaaaaa atcctctatc cggtaatctt ctcttcggat tattccgccg
     5041 cagaaaaact tcaggtctat catttctcga ttaacagtat tgatatcgcg atggaagtga
     5101 tgacccgcgc gtttaccttt agtgacagta gtgcctcaaa ggaagatgaa aattatcgta
     5161 tcttctcgtt actggaaaac gccgaagaag aaaaagaacg gcaaatagcc tcaatacttt
     5221 catgggaaat agatattatc tataaaatcc tgctggattc tgatttaggc agtagtttgc
     5281 cattaagcca ggctgatttt ggcctgtggt ttaaccataa aggtcgacat tattttagtg
     5341 gcattgccga agtaggccat atctcccgtc tcattcagga tttcgacggt attttcaatc
     5401 aaaccatgcg taacaccagg aatttgaata acagaagtct gcgggtgaaa tttttattac
     5461 agataagaaa taccgtatcg cagattatta ccttgctgcg tgaattgttt gaagaagtat
     5521 cgcgccacga agtcggtatg gatgtactaa cgaaattact taaccgccgt ttcctgccga
     5581 ctatcttcaa acgcgaaatt gcccatgcca accggaccgg tacaccgctg tcagtgctga
     5641 ttattgacgt tgataaattc aaagagatca acgatacgtg gggccataac actggcgatg
     5701 aaattctgcg taaagtctct caggcctttt atgacaacgt ccacagtagt gattatgttt
     5761 ttcgctacgg gggcgatgaa tttatcattg ttttgactga agcttctgaa aacgaaacgt
     5821 tacgtaccgc agaacgtatt cgcagtcggg tggagaaaac caaactgaaa gccgcaaacg
     5881 gcgaggatat tgccctctca ctttccatcg gtgccgccat gtttaatggt catcctgact
     5941 atgagcgcct cattcaaata gccgatgaag ctctgtatat cgccaaaaga cgaggtagaa
     6001 accgtgttga actctggaaa gccagtcttt agatgcgcca ggatgcagag gtaatcatga
     6061 agctaaccga tgcggaaaat gccgccgatg gcattttttt ccccgccctt gaacaaaata
     6121 tgatgggcgc ggtgttaatt aacgaaaatg atgaagtgat gtttttcaac cccgccgcag
     6181 agaagctctg ggggtacaaa cgtgaagaag tcattggcaa taacattgat atgctgattc
     6241 cacgggattt gcgtcctgcg catcctgaat acattcgtca taaccgtgaa ggtggtaaag
     6301 cgcgcgtcga ggggatgagt cgggagctgc agctggagaa aaaagacggc agtaaaatct
     6361 ggacccgttt tgcgctatcg aaagtgagcg ccgaggggaa agtttattac ctggcgctgg
     6421 tacgggatgc cagcgtagaa atggcgcaaa aagaacagac ccgacaattg attattgccg
     6481 ttgaccatct cgaccgaccg gtgattgtcc tcgatccgga acgccatatt gtgcagtgca
     6541 atcgcgcatt taccgaaatg tttggttact gcattagcga agccagcggt atgcagcccg
     6601 atacactcct gaacattcct gaattccctg ccgataaccg cattcgttta caacagttgc
     6661 tatggaaaac cgcccgcgat caggacgaat ttctgctgtt gacacgcacc ggtgaaaaaa
     6721 tctggattaa agcctctatc agcccggttt atgacgtgct cgcgcatctg cagaacctgg
     6781 taatgacctt ctcggatatc accgaagaac ggcagatccg ccagcttgaa ggcaatattc
     6841 tcgccgccat gtgcagcagc ccgccatttc atgaaatggg ggaaatcatt tgtcgtaaca
     6901 tcgaatctgt tctcaacgaa tcgcatgttt cgctgttcgc actgcgcaac gggatgccga
     6961 tacactgggc gtcatcttcg cacggtgcag aagttcaaaa tgcgcaaagc tggtcagcga
     7021 ccattcgtca gcgtgatggt gcgcctgcgg ggatcctgca aattaaaacc tcgtcaggag
     7081 cagaaaccag cgcctttatc gaacgcgtgg cagatatcag ccagcatatg gccgcgctgg
     7141 cgctggaaca ggaaaaaagc cgtcagcata ttgaacaact catccaattt gatccgatga
     7201 ccggtctgcc aaatcgcaat aacctgcaca attacctcga tgaccttgtc gacaaagccg
     7261 tctctccggt ggtgtatctc atcggtgttg accatattca ggatgtgatt gatagccttg
     7321 gctatgcgtg ggccgatcag gcattgctgg aagtggtcaa tcgctttcgt gaaaaactca
     7381 aaccggatca gtatctctgt cgtatcgaag gtacgcagtt tgtcctcgtg agcctggaaa
     7441 acgacgtcag taacattacc caaatcgccg atgagctacg gaatgtggtc agcaagccga
     7501 taatgattga cgataaacca ttcccgctaa ccttgagtat tggcatcagc tacgacgtgg
     7561 gtaaaaaccg cgattacctg ctctccactg ctcacaatgc aatggattat attcgcaaga
     7621 atggcggtaa cggctggcag ttcttcagcc cggcgatgaa cgaaatggta aaagagcgtt
     7681 tggttttagg cgcagcgctg aaagaagcga ttagcaataa ccaactgaaa ctggtttacc
     7741 agccgcaaat cttcgcagaa acgggtgaac tgtacggcat cgaagccctt gctcgctggc
     7801 acgatcccca gcatggtcat gtaccccctt cacggtttat tcctctcgca gaagagattg
     7861 gtgaaatcga aaatattggg cgctgggtca tcgcggaagc ttgccgtcag ttagcagaat
     7921 ggcgtagcca gaatattcat atcccggcgt tatccgtgaa cttgtcggcg ctgcactttc
     7981 gcagtaatca gctgcctaat caggtgtctg atgcaatgca agcctggggt attgacggcc
     8041 accagctgac ggtagaaatc acggaaagca tgatgatgga acacgatacc gaaatcttta
     8101 agcgcattca gatcctgcgc gatatgggcg tgggcttatc ggtagatgat tttggcacgg
     8161 gcttttccgg attatcccgc ttagtcagtc ttccggtaac ggaaatcaaa attgacaaaa
     8221 gttttgtcga tcgttgtctg acggaaaaac gcatccttgc cttacttgaa gccattacca
     8281 gcattgggca aagcctcaat ttaaccgtcg tggcggaagg cgtcgaaacc aaagagcaat
     8341 ttgagatgct acgcaagatc cactgtcgcg ttattcaggg atatttcttt tcccgccccc
     8401 tacccgccga ggaaattcca ggctggatga gcagcgtgtt accgctgaaa atctgacaaa
     8461 ttcctctcgc ccgcactcgc gggttttcat ttaacgtgac gctgtcactt aaatactgtg
     8521 atttcagcta cgattccggg agattccttc cttaacttcc cctcttgcac caaaactgca
     8581 ctacgctgca caaatgttga tcaaaagtta aaaaatataa ctttaagcca gcattctggc
     8641 gcttatcccg gcatggcatg agatctgcat aagcggaaag cgcagcaatt tttgtcttaa
     8701 tacaggaacc gtttatgtcg gataccaccg aactggttga tttagccgtg atcttccctg
     8761 atctggagat cgaattgaaa tacgcctgcg ctgataacat cacaggtaaa gctatttacc
     8821 agcaagcgcg ttgtctgtta cacaaggatg cgattaccgc gctggcgaaa agtatcagta
     8881 tcgcccagct gtcaggctta caactggtga tttacgatgc gtatcgccca caacaagcac
     8941 aggcgatgtt gtggcaagcc tgcccagacc cgcaatatgt tgttgatgtg acggtcggtt
     9001 ctaatcacag ccgtggcacg gcgatcgacc tgacgcttcg tgatgagcac gggaacatcc
     9061 tggatatggg cgcagggttc gatgaaatgc acgagcgctc ccatgcctat cacccttccg
     9121 tcccgcccgc cgctcagcgc aatcgcctgt tgctgaatgc gataatgact ggtggcggtt
     9181 ttgtcggtat ctccagcgaa tggtggcact tcgaattacc ccaggcagcg agttaccctc
     9241 tgctcgctga tcaattctct tgttttatat cccctggcac acagcacgtc agttaatcag
     9301 gaaactgtca tgaagagatc gatatcgttt cgtcccacat tgctcgcgct cgtccttgcc
     9361 acaactttcc cggttgcgca cgccgccgta ccgaaagata tgctggtgat tggtaaggcc
     9421 gccgatccac aaaccctcga cccggcggta acaatagata ataacgactg gacagtgacc
     9481 tacccgtctt atcagcggct ggttcagtac aaaacggacg gtgataaagg ctcaaccgac
     9541 gttgaaggcg atctggcaag tagctggaaa gcgtctgacg atcaaaaaga gtggacgttc
     9601 accctgaagg acaatgctaa atttgccgat ggcacacctg tcactgccga agcagtaaaa
     9661 ctctcttttg agcggttact aaaaatcggc caggggccag cagaagcatt tcccaaagat
     9721 ttaaagattg atactcccga cgaacatacg gtgaagttta cccttagcca accattcgca
     9781 ccgttcctct acacgctagc gaatgacggt gcatccatta tcaatccggc ggtcttaaag
     9841 gaacatgcag cggatgatgc tcgcggcttc ctcgcgcaaa ataccgccgg ttccggacca
     9901 tttatgctga aaagcgggca aaaaggtcag caattagttc tggtgccaaa tccgcattac
     9961 cccggcaata aaccgaactt taagcgagta tcggtaaaaa ttatcggtga aagtgcctcc
    10021 cgtcgcctgc agctctcccg tggcgacatt gacattgccg atgcgctgcc ggtggatcaa
    10081 ctcaacgccc tgaagcagga aaataaagtc aatgtggcag agtatccgtc actgcgcgtt
    10141 acctatctgt atctgaataa cagcaaagcg cctcttaatc aggcggatct gcgtcgggcc
    10201 atttcctggt ctaccgatta tcagggcatg gttaacggca ttctgagtgg taacggaaaa
    10261 cagatgcgcg gcccgattcc ggaaggcatg tggggctacg atgcgacggc aatgcaatac
    10321 aaccatgacg aaacgaaagc caaagccgaa tgggataaag tgacgagcaa acccaccagc
    10381 ctgacgtttc tctactccga taacgatccg aactgggagc ctattgctct ggcgacacaa
    10441 tccagtctca acaagctggg catcaatgtg aagctggaaa agctggcgaa cgccaccatg
    10501 cgcgacagag tgggtaaagg tgattacgac attgcgattg gcaactggag tccggatttt
    10561 gccgacccgt atatgtttat gaattactgg tttgagtcag acaaaaaagg tctgccgggt
    10621 aaccgctcgt tctatgaaaa cagtgaggtc gataagttac tgcgcaatgc gcttgcgacc
    10681 accgaccaga cgcagcgtac ccgggactac cagcaggcac agaaaatcgt cattgatgac
    10741 gctgcttatg tgtacctgtt ccagaaaaac taccaactgg cgatgaacaa agaggtgaaa
    10801 ggctttgtgt tcaatcccat gctggaacag gtcttcaata tcaataccat gagtaaataa
    10861 cgtgaccttc tggagtattt tacgccaacg ctgctggggg ctggtgctcg tggtggcggg
    10921 cgtctgcgtg attacgttta ttatctcgca tctgatccct ggcgatccgg cgcggttact
    10981 ggcgggtgac cgcgccagcg atgctatcgt ggaaaatatt cgccagcaac tgggactgga
    11041 ccagccactg tatgtacagt tttaccgcta cttcagcgac ctgtttcatg gtgacctggg
    11101 aacatccatt cgtaccgggc gtccggtgct ggaagagttg cgtatatttt tcccggcgac
    11161 gctggaactg gctttttgtg ccctgctgct ggcactcctg attggcatcc cgctgggcat
    11221 actctctgca gtctggcgaa atcgctggct ggatcatctg gtgcgaataa tggccattac
    11281 cggaatctcc acacctgcgt tctggcttgg actgggcgtc attgtgctgt tttatggtca
    11341 tctgcaaatt cttcccggcg gcggaaggct tgatgactgg ctggatccac caacgcacgt
    11401 taccggcttt tatctgctcg atgcgctgct tgaaggcaac ggtgaagtct tcttcaatgc
    11461 gttgcaacat ctcatcttac cggcattaac gctggcgttc gttcacctgg gaattgtcgc
    11521 tcgccagatc cgctcagcga tgctggaaca attgagtgaa gactacattc gtaccgcccg
    11581 ggccagcggc ttgcccggct ggtatatcgt tttatgttat gcgctaccca atgcgttgat
    11641 cccatcgatt accgtattgg gtctggcgct gggcgatttg ttgtatggcg cagtgctcac
    11701 cgaaaccgtt tttgcctggc ccggaatggg tgcatgggta gtaacatcaa tacaggcgct
    11761 cgacttcccg gcagtgatgg gctttgccgt cgtggtttca tttgcttatg tgctggtcaa
    11821 cctggtggtg gatttgctct atttgtggat tgatccgcgt atcggacgtg gaggtggtga
    11881 atgatgctaa gcgaggaaac gtccgccgta cgcccacaaa aacaaacgcg atttaacggt
    11941 gcaaaactgg tatggatgct gaaaggcagt ccgctcaccg tgaccggcgc agtcatcatt
    12001 gtattaatgc tattgatgat gattttttca ccgtggcttg cgacgcatga tcccaacgcc
    12061 attgatttaa ccgcccgcct tttgccgcct tctgcggcgc actggtttgg caccgatgaa
    12121 gtgggacgcg atctgttcag ccgcgtactg gtcggcagtc agcaatcaat tctcgccgga
    12181 ttagtcgtgg ttgccattgc gggtatgatt ggttcgctac tcggatgtct atccggtgtg
    12241 cttggcggac gcgcagacgc catcatcatg cgcatcatgg acattatgct gtcgattcct
    12301 tcgttggtac tgacaatggc actggctgcc gctctcgggc cgagtttatt taacgccatg
    12361 ctggcgattg ctattgtgcg aattcccttt tatgtgcgcc tggcgcgggg gcaagcatta
    12421 gttgtacgcc agtataccta tgttcaggcg gcgaaaacct ttggtgcgtc gcgttggcat
    12481 ctgatcagct ggcatatttt acgtaactcc ctgccgccgc tgatcgtgca ggcatcgctg
    12541 gatatcggta gcgcgatttt aatggccgcc acgttgggat ttattggcct gggtgctcaa
    12601 caaccgagtg ctgaatgggg cgcgatggta gctaatggtc gcaactatgt actcgatcag
    12661 tggtggtatt gcgcatttcc gggggcagcg attttgctta ccgccgtcgg gtttaatctc
    12721 tttggtgatg gtattcgcga tctgcttgac ccgaaagcag gaggaaagca gtcatgaccc
    12781 aacccgttct ggacattcaa cagctgcatt tgagtttccc cggttttaac ggttatgttc
    12841 acgtgctcaa caatgtgtcc ttgcagatta accgcggtga aattgtcggt ctggtgggag
    12901 aatccggctc aggtaaatca gtcaccgcaa tgctgattat gcgtctgcta ccgacgggca
    12961 gttattgcgt acatcggggg cagatttcac tgctgggaga cgatgttctt aacgctcggg
    13021 agaaacagct tcgtcagtgg cgcggcgcac gagtggcgat gatctttcag gaaccgatga
    13081 ccgccctcaa tccgacacgt cgaataggtc ttcagatgat ggacgtgatc cgccatcatc
    13141 aaccaataag tcgtcgggaa gccagagcta aagcgattac cctgctggaa gagatgcaaa
    13201 tcccggatgc cgtggaagtt atgtcgcgct atccgtttga gctttcaggt ggtatgcgcc
    13261 agcgggtaat gattgcgctg gcattctcct gcgagccgca attgattatt gccgacgaac
    13321 cgactacggc gctggacgtc acggtacagt tgcaggtact gcgtttgctt aaacataaag
    13381 ctcgcgccag tggaactgcg gtactgttca tcagccatga tatggccgtg gtgtcgcaac
    13441 tgtgcgatag cgtttacgtg atgtatgccg gaagcgtaat tgaaagcggc gtgacggcag
    13501 acgttatcca tcatccccgg catccgtata ccattggttt gctgcaatgc gcaccagaac
    13561 atggagtacc acgccagcca ttacccgcca ttccagggac ggtaccaaac ctcacccatt
    13621 tgcctgacgg ctgcgctttt cgcgatcgtt gctatgcggc aggtgcacag tgtgaaaacg
    13681 tcccggcgct gacagcgtgt ggtgacaaca accagcgctg cgcctgttgg tatccgcagc
    13741 aggaggtcat aagtatctga cacgttatta acgttacgcg acgtccatat caatttcccg
    13801 gcccgtaaaa gctggcttgg taaaactacg gaacatgttc atgcgattaa tggtattgat
    13861 ttacagatcc gccgtggtga aaccttaggg atcgtcggtg agtcaggctg cggcaaaagc
    13921 accctcgcac agcttttaat gggtatgctg caaccgagcc acgggcaata catccgttca
    13981 ggatcacaac gcattatgca gatggtgttt caggacccgc tctcttcgct taatccgcgc
    14041 ttatcggtgt ggcgcatcat cacagaaccg ctctggatag ctaagcgtag tagtgaacaa
    14101 cagcggcgag cgttggcaaa ggagttggct gtgcaggtgg gtattcgtcc ggagtatctc
    14161 gaccgcctgc ctcatgcgtt ctccggcggg cagcggcaac gcatcgccat tgccagagca
    14221 ctctcttcgc agcctgacgt gattgtgctt gatgagccaa cctcggcgct ggatatctcc
    14281 gtgcaggcgc agatcctcaa tttactggta acgctacagg aaaatcacgg gctgacttat
    14341 gtgctgattt cacacaatgt ctcggtgata cgtcatatga gcgatcgggt ggcggtgatg
    14401 tatctcgggc agattgtaga actgggcgac gcgcagcagg tgctgacggc acctgcacat
    14461 ccatacaccc gattattgct ggattccctc cccgccattg ataaaccgct ggaggaagaa
    14521 tgggcattat gtaaaacgga tctgccggga aaccgcacgt tgccacaagg ctgttttttc
    14581 tacgaacgtt gccctctggc aacccacgga tgtgaagtcc ggcaatcatt agcaataagg
    14641 gaggacggac gtgagctccg gtgctggcgg gcgctgtaga gatgctaaca acgcatcagg
    14701 cattcacgcg cctgacgcgt catccggcaa tgctttacga tttcaactgg taatccagcg
    14761 taatttccgc tttcagtacc tgagaaaccg ggcatcctgc ttttgctttc tggatgatgc
    14821 cgtcaaaggt agaggcatca atacccggca ccgcaacttc actcttcagt gcgattttcg
    14881 taatcgcaaa atcggcatcc actttatcca gcgacacatc ggcggtggta tcaatcgatg
    14941 ttggcgtgaa tcccgcttcc cccagcatta atgaaagcgc cattgagaaa catgcggcat
    15001 gcgctgcgcc aatcagttct tcagggttgg ttcctttttc gccttcaaaa cgcgtgttaa
    15061 atccatacgg ctgttggttc agcacgccac tctgggtgga tactgttccc ttcccgcgtt
    15121 tgatatcgcc ttcccagtgt gcctgacctt tcttatggat tgtcattgtt gctctcctgt
    15181 gggcttaaaa aggaaattat agactacgtg agaaacagca cgaggataag caggatattc
    15241 cgaataaaat acggcgaaaa tattacttct gcacttaaat taaattccca caatagataa
    15301 ccgggaatat atatctcaag agatattgca tcaatatcaa tcaactcacc acaataataa
    15361 tacatatcaa tatcatggag ttaaaatata aaaatcatca accaggacta atcttaacaa
    15421 cgaagcggca aatatttgcc atgctgaatg tgctttataa atctgcgatc cgtagcagac
    15481 accataaata cacagacacg gagaatcact atgtttactt attatcaggc agaaa
//
LOCUS       DAETQN010000103        15407 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.103, whole genome shotgun sequence.
ACCESSION   DAETQN010000103 DAETQN010000000
VERSION     DAETQN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.103"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            11..454
                     /locus_tag="K0W68_003186"
     CDS             11..454
                     /locus_tag="K0W68_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3074673.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            670..759
                     /locus_tag="K0W68_003187"
                     /pseudo
     CDS             670..759
                     /locus_tag="K0W68_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(760..1818)
                     /gene="modC"
                     /locus_tag="K0W68_003188"
     CDS             complement(760..1818)
                     /gene="modC"
                     /locus_tag="K0W68_003188"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBI3074674.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1821..2510)
                     /gene="modB"
                     /locus_tag="K0W68_003189"
     CDS             complement(1821..2510)
                     /gene="modB"
                     /locus_tag="K0W68_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBI3074675.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2510..3283)
                     /gene="modA"
                     /locus_tag="K0W68_003190"
     CDS             complement(2510..3283)
                     /gene="modA"
                     /locus_tag="K0W68_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3074676.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="K0W68_003191"
     CDS             complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="K0W68_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBI3074677.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3727..4515
                     /gene="modE"
                     /locus_tag="K0W68_003192"
     CDS             3727..4515
                     /gene="modE"
                     /locus_tag="K0W68_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBI3074678.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4583..6055
                     /gene="modF"
                     /locus_tag="K0W68_003193"
     CDS             4583..6055
                     /gene="modF"
                     /locus_tag="K0W68_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBI3074679.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6316..7332
                     /gene="galE"
                     /locus_tag="K0W68_003194"
     CDS             6316..7332
                     /gene="galE"
                     /locus_tag="K0W68_003194"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBI3074680.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7342..8388
                     /gene="galT"
                     /locus_tag="K0W68_003195"
     CDS             7342..8388
                     /gene="galT"
                     /locus_tag="K0W68_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBI3074681.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8392..9540
                     /gene="galK"
                     /locus_tag="K0W68_003196"
     CDS             8392..9540
                     /gene="galK"
                     /locus_tag="K0W68_003196"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBI3074682.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9534..10574
                     /gene="galM"
                     /locus_tag="K0W68_003197"
     CDS             9534..10574
                     /gene="galM"
                     /locus_tag="K0W68_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBI3074683.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10777..11529
                     /gene="gpmA"
                     /locus_tag="K0W68_003198"
     CDS             10777..11529
                     /gene="gpmA"
                     /locus_tag="K0W68_003198"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBI3074684.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="K0W68_003199"
     CDS             complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="K0W68_003199"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBI3074685.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13056..13436
                     /locus_tag="K0W68_003200"
     CDS             13056..13436
                     /locus_tag="K0W68_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HBI3074686.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13550..14491
                     /gene="zitB"
                     /locus_tag="K0W68_003201"
     CDS             13550..14491
                     /gene="zitB"
                     /locus_tag="K0W68_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBI3074687.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="K0W68_003202"
     CDS             complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="K0W68_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBI3074688.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15245..>15407)
                     /gene="nadA"
                     /locus_tag="K0W68_003203"
     CDS             complement(15245..>15407)
                     /gene="nadA"
                     /locus_tag="K0W68_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBI3074689.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3916 a         4044 c         3851 g         3596 t
ORIGIN      
        1 cggcctttgc atggatcttg cactggtaac aggacagcgc gaaggagatc tggcggctat
       61 gagatgggag gatataagag atggcaggct atacgtggaa caacaaaaaa caggggcaaa
      121 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      181 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      241 gacaatcgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      301 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      361 cggtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      421 tgacggacga gggagggaat ggaaagacat ttaaaattaa tttcgaatga tttcgaaaca
      481 ccacttgtaa ttaattgatt ataaatgatt aattcctaat gggcatttgc ttcatgaatg
      541 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      601 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      661 aaattcttaa atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
      721 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
      781 tttaatttgc gcgtacagcc acaggccagg tttgatcgcc agttcatccc tggcccacgg
      841 gctgatacgc gcccacagcg ttttaccgcc gacttccagc tccacttcca cctggccgtt
      901 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
      961 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
     1021 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc
     1081 gtaatgcgga tgatgctcaa gtaccgtcac tttcagaatg ctactttgtt gctctttcgg
     1141 cagccacgga ttcatcacgc tactgcccca cacttcctcc agcgcgccaa aggctttcac
     1201 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
     1261 gctgacatac aacatcggaa tgttgatttc ccgtgtcagc cgttgcagat aaggcaacag
     1321 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattccgg
     1381 tgctgtcagc aaagcccgac caatcgccac gcgctgtttt tcgcctccgg acaggctgcc
     1441 tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
     1501 gaccatactt ttcgacatgc cgtagcgcag attgccacgc actttgtaat gcgggaacag
     1561 ccgcgcatcc tgaaaaacat agccaacgcg acgcttttcc ggcgtcaggc agataccttt
     1621 ttcggcatca tttagtaccc gcccattgag gacaatccgc cctttttgcg ggcgcgtcag
     1681 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
     1741 gatgccattg gcgggcagtg tttcattaat ggtcaggcaa tggttgccca acgtctggga
     1801 aaaattcagc tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
     1861 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg
     1921 ccactttccc cgccgggggt ctggatcagg gtatacatgg cagagggaat ggttcgcgtt
     1981 tcgccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca
     2041 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
     2101 aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct gttcgagttt gacatcaacc
     2161 ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg
     2221 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
     2281 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
     2341 aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc
     2401 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
     2461 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
     2521 taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg
     2581 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
     2641 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac
     2701 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
     2761 cttccgccgg agccagtttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
     2821 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat
     2881 tcagcagcga agtccagtta gttgtgctgt cgatgatgaa atctttctgc tcgctggctt
     2941 tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg
     3001 cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag
     3061 gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat
     3121 ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
     3181 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag
     3241 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt
     3301 aacgttatat aggtgacaat gtaacggcta gctcagaatt ttcccagcag ttattgctaa
     3361 cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa
     3421 atgcccgact aagcgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac
     3481 caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg
     3541 ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg
     3601 tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct
     3661 tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac
     3721 attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac
     3781 ccgcgccgga tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg
     3841 aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta
     3901 agcgagcata ttctggttga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg
     3961 acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa
     4021 gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc
     4081 tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc
     4141 gatcatgatg acattcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg
     4201 aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg
     4261 ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac
     4321 gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa
     4381 gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg
     4441 acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc
     4501 gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc
     4561 gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct
     4621 tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc
     4681 gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact
     4741 tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga
     4801 gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc
     4861 tggcgaagat gacaccggac gcactacggc tgaaatcatt caggatgaag taaaggatgc
     4921 accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt
     4981 taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc tgatgtcgga
     5041 gcctgacttg ttgattcttg atgagtcgtt cgatggcctg gatgttgcct cacgtcagca
     5101 gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa
     5161 tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt
     5221 agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcgca
     5281 tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc
     5341 cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg
     5401 ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg
     5461 gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg
     5521 ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga
     5581 tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac
     5641 taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt
     5701 ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg
     5761 tacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg cgctgatcgt
     5821 ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga
     5881 tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac
     5941 gcaattgtta tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct
     6001 tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg
     6061 tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg
     6121 caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt
     6181 ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa
     6241 tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc
     6301 ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat tggaagtcat
     6361 acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac
     6421 agtaagcgca gcgtactgcc tgttatcgag cgcttaggcg gcaaacatcc aacgtttgtt
     6481 gaaggcgata ttcgtaacga agcgttgatg accgagatac tgcacgatca cgctatcgac
     6541 accgtgatcc acttcgccgg gctgaaagcc gtcggcgaat cggtacaaaa accgctggaa
     6601 tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg cgccgctaac
     6661 gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca
     6721 tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag caaattgatg
     6781 gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat tgccctgctg
     6841 cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc
     6901 attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg
     6961 gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac
     7021 gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc
     7081 gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt ggttaatgcc
     7141 ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg cgaaggcgac
     7201 cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg
     7261 cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga
     7321 tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc
     7381 tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg
     7441 cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc
     7501 ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac cgggacttac
     7561 gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac
     7621 gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg
     7681 gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc
     7741 tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac
     7801 aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc
     7861 ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag
     7921 aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt
     7981 gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg
     8041 ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc
     8101 gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc
     8161 ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg
     8221 cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg
     8281 gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag
     8341 cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg
     8401 aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt
     8461 caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga cggtttcgtt
     8521 ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa
     8581 gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc
     8641 attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg
     8701 caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag
     8761 ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag
     8821 ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac
     8881 cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa
     8941 gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc catgccgaaa
     9001 ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac
     9061 aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agctctgcgc
     9121 gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa
     9181 cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag
     9241 caaggcgatc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat
     9301 gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agcagtgatt
     9361 ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgctg
     9421 atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa
     9481 acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga
     9541 acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca
     9601 acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca gctcgtattc
     9661 cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc
     9721 aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata
     9781 gccgttatac ctttgacggt gaaacggtga cgctttcgcc aagtcagggc gttaaccagc
     9841 tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc
     9901 gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg
     9961 gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca
    10021 cagttgataa atcttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg
    10081 agcagtctga cgtgcgcaat cacaagttgc agattctggc gggcgaatat ctgccggttg
    10141 atgaaggcgg cattccgcac gacggcctga aatctgtcgc cggaacctct tttgatttcc
    10201 gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag
    10261 gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg
    10321 tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccc gctcttcaat
    10381 tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct tacgccgact
    10441 ggcaaggact ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc
    10501 cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt
    10561 ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat tgttcaaatc
    10621 accagcaaac accgacatat ttgcaactca atattcacaa caaccttaca ctgcgccact
    10681 attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat ataatgagaa
    10741 ttattatcat aaaaagataa tttgaggagt aagtatatgg ctgtaactaa gctggttctg
    10801 gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg gtacgacgtg
    10861 gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct gaaagaggaa
    10921 ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca taccctgtgg
    10981 aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg gaaactgaac
    11041 gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaaccgccga aaagtatggc
    11101 gacgagcagg tgaaacaatg gcgtcgtggt tttgccgtga ctccgccgga actgactaaa
    11161 gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga gaaagagctg
    11221 ccgctgactg aaagcctggc gctgaccatt gaccgcgtga tcccttactg gaatgaaact
    11281 attctaccgc gtatgaagag cggtgagcgc gtgatcatcg ctgcacacgg taactcttta
    11341 cgtgcgctgg tgaaatatct tgataacatg agtgaagaag agattcttga gctgaatatc
    11401 ccgaccggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct gaaacgctat
    11461 tatctgggta atgctgacga gatcgcagcg aaagcagcgg cggttgctaa ccagggtaaa
    11521 gcgaagtaaa cgtcattcgt ttaaaaatga gaaagccgac tgcaagtgag tcggcttttt
    11581 tgttgctaac aatggagcac attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg
    11641 tgattcatcg gatacgccac tctgacggcg catccgacaa ttaaacctta cccgcgacgc
    11701 gcttttactg cattcgccag ttgacgtaac agagcatcgg tatcttccca gccaatgcag
    11761 gcatcggtga tgctcttacc gtaggccagc ggctccccgc tatcgaggct ctgattgcct
    11821 tccaccagat ggctttccac catcacgcca ataatggcct tttcgccacc ggcaatctgc
    11881 tggcaaacgt cagcacaaac atccatctgc tttttgaatt gtttggacga gttagcatgg
    11941 ctgaaatcga tcatcacctg tgctggcagg cctgctttgt tcagcccttc tttcacttca
    12001 gcaacgtgct tcgcgctgta gttaggctct ttaccgccgc gcagaatgat atggcaatca
    12061 ccgttaccgc tggtattcac aatcgccgaa tgcccccatt tagttacgga caggaagcag
    12121 tgcggcgcac cggcggcatt aatggcatcg atagccactt taatcgtacc gtctgtgcca
    12181 tttttaaaac ctaccggaca agaaagacca gacgccagtt cgcggtgcac ctgcgattcc
    12241 gtggtacgtg caccaattgc gccccagctc atcaggtcag cgagatactg aggagtgatc
    12301 atatcgagaa actcacccgc cgctggcaga ccgctgtcgt taatatcaag cagcaattta
    12361 cgggctatac gcagaccgtc gttgatctgg aagctgttat ccatatgcgg atcgttaatc
    12421 aaccctttcc agcccaccgt ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc
    12481 agctcatctt tcagctcttc acgcagcgcc agcaagcgag tggcatactc tttagccgcg
    12541 acaggatcat gaattgagca cgggccaatc accaccaaca ggcgatcatc attacctttc
    12601 aggatcttat ggatcgcttt tcgggcatgg gcaaccgtat tcgcggcatt ttcagttacg
    12661 gggaattttt ccagcaatgc gacaggagga agtaactctt tgatttcttt gatgcgtaaa
    12721 tcgtcgttct gataattcat gtctgttcca gtgttgccat acttatctta gtgaatgcaa
    12781 tacttccaat ctatatcttc cgtcagaatg tgtaaacggg gttttacact atgaacggat
    12841 taatcctgga attagcaaga aaaacgccag attgtcgcga aaaacgagat ctctcctaca
    12901 atttctaact gtaactcctt tgtttgttaa ttatttcaag attctctgct gcgtttcata
    12961 aactggctga aatcttaaac caatgcctta tattcacctg caaatgcact gttggaagag
    13021 gttatccgac ataacgacca taacaggagc atcctatgaa aatgacaaaa ctggccacat
    13081 tatttctgac tgccactcta agccttgcca gcggtgccgc actggctgcc gatagcggag
    13141 cgcaaactaa taacggccag gcaaacgccg cagctgatgc gggccaggta gcccctgacg
    13201 cccgtgaaaa tgtcgcgcca aataacgtcg acaataacgg ggtaaatacc ggttctggcg
    13261 gcacaatgct gcattcggat ggttcttcaa tgaacaatga cggaatgacc aaagatgaag
    13321 agcacaagaa caccatgtgc aaagatggtc gatgtccgga tatcaataaa aaggtacaaa
    13381 tcggtgatgg catcaataat gatgtcgata ccaaaaccga cggtaccacg cagtaaccct
    13441 gcaaaatagc caataatggg agagcggagt ctctcccttt taatttttca cttctaaaag
    13501 cagtatgatg tcataaagtt aagtgagaat aattaaggga tgactcgtca tggcgcactc
    13561 acactcacac acgtcctcac acctgccaga agataataat gctcgtcgct tgttgtatgc
    13621 tttcggcgtg actgccgggt ttatgctggt agaagtcgtt ggtggttttc tttctggttc
    13681 tctggcattg ctggccgatg cgggtcatat gttgaccgat actgccgccc tgcttttcgc
    13741 cctactcgcc gtgcaatttt cacgtcgtcc tcccaccatt cgccacactt tcggttggct
    13801 aagactgacc acgctcgcgg cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt
    13861 gattgtctgg gaggcgatag aacgcttccg cacgccgcgt ccggtcgagg gcggcatgat
    13921 gatggcaatt gccgtggctg ggttgctggc aaatatactt tctttctggt tacttcatca
    13981 cggcagcgaa gagaaaaacc tcaacgtacg agcagcggca ttgcatgtgc tcggggacct
    14041 gttgggttcg gttggggcta ttattgccgc gttgattatt atctggaccg gctggacacc
    14101 tgctgacccc attctctcga tagtggtgtc gcttctggtt ctgcgtagcg cgtggcgact
    14161 gttgaaagat agcgtgaatg aattacttga aggtgcaccg gtatcgctgg atatcgctga
    14221 actgaagcgt cgtatgtgcc gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt
    14281 atggatggta ggcgagaagc cggtgataac gctgcatgtg caggtgatcc cgccacacga
    14341 tcacgatgcc ttgttggatc agatccaaca ctacctgatg gatcactatc agattgagca
    14401 cgccaccatt cagatggaat atcaaccttg tcatgggcca gactgccacc tgaatgaagg
    14461 ggtgtctggc cattcacatc atcaccatta atgggacagc gcgcgtgagc ctctttcacg
    14521 tgcgctgttg atccacatcc gactgccgtt gagcgcaata aaggtcagga tgatgtactc
    14581 cagagacatg gcgtaaacgc cctgaagtgc aaaaataacg acgctaatca cgttaataat
    14641 cacccacaac agccagtttt ccacatactt acgcgtcatc agaatcattg ccacgataga
    14701 taacaccatc atgcatgaat cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac
    14761 aacctgtaat cctaatgctt gcatgatcat gactgccacg cgggtcaaaa atgcaaacac
    14821 cggattgata aataccgtca tcagaccaat cgaaacaacg caaaccgcca accagctgag
    14881 cgccttcggc aatggtaacc agcgaatttt caactccgcc tcgttctgac tggtttgtcg
    14941 cgaccacgca taccaaccgt aaatattcgc ggcaaagaaa aacacctgta atagcaggct
    15001 ggcatacagc tgaatctgaa agaaaataat gccaaacaag gtgacgttga tcaggccaaa
    15061 gaagtagttg ctgatcttct ccagactggc aaggccaata cacagcaacc cggcgatcgt
    15121 gcctaccgct tcgatccatg agagatcata accgcctgcc cctattggta tatgtaccag
    15181 gatattctgc acactaaaaa aatccatctt tcccccaaag cgtaacgtta cgccttatta
    15241 ttcgttatcc acgtagtgta gccgcaaaat ccagcatacg attgagcggc accagcgccc
    15301 tctctcgcag cctttcatca agaacctcgt gattgcttcc ttcctgttct aatgcctctg
    15361 cgatggactg aaggccattc atggccatcc acggacaatg cgcgcag
//
LOCUS       DAETQN010000104        15337 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.104, whole genome shotgun sequence.
ACCESSION   DAETQN010000104 DAETQN010000000
VERSION     DAETQN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.104"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            99..851
                     /locus_tag="K0W68_003204"
                     /pseudo
     CDS             99..851
                     /locus_tag="K0W68_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
     gene            complement(892..1824)
                     /locus_tag="K0W68_003205"
     CDS             complement(892..1824)
                     /locus_tag="K0W68_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBI3074690.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2188..4974
                     /gene="polA"
                     /locus_tag="K0W68_003206"
     CDS             2188..4974
                     /gene="polA"
                     /locus_tag="K0W68_003206"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBI3074691.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5356..5988)
                     /locus_tag="K0W68_003207"
     CDS             complement(5356..5988)
                     /locus_tag="K0W68_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBI3074692.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6570..7079
                     /gene="yihI"
                     /locus_tag="K0W68_003208"
     CDS             6570..7079
                     /gene="yihI"
                     /locus_tag="K0W68_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBI3074693.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7268..8641
                     /gene="hemN"
                     /locus_tag="K0W68_003209"
     CDS             7268..8641
                     /gene="hemN"
                     /locus_tag="K0W68_003209"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBI3074694.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8831..8941)
                     /locus_tag="K0W68_003210"
     CDS             complement(8831..8941)
                     /locus_tag="K0W68_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBI3074695.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9053..10462)
                     /gene="glnG"
                     /locus_tag="K0W68_003211"
     CDS             complement(9053..10462)
                     /gene="glnG"
                     /locus_tag="K0W68_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBI3074696.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10474..11523)
                     /gene="glnL"
                     /locus_tag="K0W68_003212"
     CDS             complement(10474..11523)
                     /gene="glnL"
                     /locus_tag="K0W68_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBI3074697.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11697..13106)
                     /gene="glnA"
                     /locus_tag="K0W68_003213"
     CDS             complement(11697..13106)
                     /gene="glnA"
                     /locus_tag="K0W68_003213"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBI3074698.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13479..15302
                     /gene="typA"
                     /locus_tag="K0W68_003214"
     CDS             13479..15302
                     /gene="typA"
                     /locus_tag="K0W68_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBI3074699.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         4004 a         3829 c         3937 g         3567 t
ORIGIN      
        1 cagtagctga acaggaggga cagctgatag aaacagaagc cactggagca cctcaaaaac
       61 accatcatac actaaatcag taagttggca gcatcaccag cgagtcgaaa tttaatcaga
      121 ttaacattat caatcacggg gaagacgttg cccaaatcga tgctttcgtt gcaaaaacca
      181 tgctggatcg cgttaaagac aaagattata tcaatgtcaa tctgacctac gaacttgata
      241 agttaacaaa agggaatcag caactcggta gtggtgagtg gtcattgatt gctgaatcta
      301 ttgatccctc agcagtgcgc caatttatca tccagtataa cattgctatg cagaagcagc
      361 ttgctgtaca ccctgagtta gcaaacgatg aagttgctct gcaagaagtg aatgctgcat
      421 tgttcaaaga gtatttaccg ttattacaaa aaagtgagcc gaccattaaa caaccggtaa
      481 gatggaagaa cgcactcggc gaactaaatg ccaatctgga tatcagtatt gccgacccag
      541 ccaaatcttc atcatccaca aacaaagata tcaaattgct caattttgat gtgaagttac
      601 cgcttaatgt cgtcacagaa accgcaaaac agcttaattt atctgaagga atggatgcgg
      661 aaaaagcgca aaagcaggct gataaacaaa tcagcgggat gatgacattg ggtcagatgt
      721 ttcagttaat cacgattgac aacaataccg cctcgctgca gctgcgttat acaccgggta
      781 aagttgtgtt taacggacag gagatgagcg aagaagaatt tatgtctcgt gccggacgtt
      841 ttgttcatta aaatactcac tcgcctctgc tcattcagag gcgagtaaaa cttacttatt
      901 ctgacgctgt gatgacatga gagacgtcag aagccggtct ttttcctgcc acaatgagtt
      961 cagccattgc tgaaaatggc gcttaaagct tttgtcattg atgtagtcgc catgtaactc
     1021 atcggcaata ggctgtaaat ccacatgtac gacaatccgc gtcagtttac cgcttaacat
     1081 atcgaagaac ggctgacggt tattgtccgg ataacacagc gtaacattaa gcagtttatc
     1141 gaattgttta cccagtacat ttagcgccat cgcaatgcct gccgcctttg gtggcaacaa
     1201 gttttgaaaa gtggagtggg tttgctgatg tttttcttgc gtgaaacggg agccttcaac
     1261 gaaattgaca atggtggtgg gatgcaggcg aaacttttca caagaacgcc gagtggtttc
     1321 aacatcttta ccacgtcgct ccggatgacg taataaataa gcgcgggaat aacgcttcat
     1381 aaacggcata tccagcgccc agcacgccag gccaagaaat ggcacccagg ccagctgttg
     1441 cttgaggaaa tatttattca tcggaatgtg cttacgaaac agcacgcaca gtacgacaat
     1501 atctgcccaa ctacggtggt tacaaataag cagataccag ttcttcttac ttaacccttc
     1561 cagcccgtga acttcccatt gcaggtgtgg gttcagatgc agtaataccg ccagaccttc
     1621 acaccagcaa tacatcataa aatcacaaaa acgcgaaacc tttcgccaaa tgactggcac
     1681 aggtagcaat agttttacaa tcccggcaat gatgatcggg acggaacaaa aaatagtgac
     1741 caaaatggtc aatacgatac tcagcaaaag ggttatcgca gcgagtattc tcgtcataat
     1801 gaatttattc aaaaggttag ccatagacag tgcgctaaag aagcgcaagg cgcagatttt
     1861 agcagaaaac gacccaaata acggatgatc cttaaggaga aaaataatcc atatctatcc
     1921 acattagaaa aaatcccatt atctcaatta ttagggatgg ctttattttt aactgcatga
     1981 aaaacaaaga caaacatcat gctgtaaaaa gcatgataat aaattaaaag cgatgtaaat
     2041 aatttatgca caaagttatc cacatgacga tttgcgagcg atccagaaga tctacaaaag
     2101 attttcacga aaagcggtga aaaactcatg ttttcatcct gtctgtggca tcctttaccc
     2161 ataatctgat aaacaggcac ggacattatg gttcagatcc cccaaaatcc acttatcctt
     2221 gtagatggtt catcttatct ttatcgcgca tatcacgcgt ttcccccgct gactaacagc
     2281 gcaggcgagc cgaccggtgc gatgtatggt gtcctcaaca tgctgcgcag tctgatcatg
     2341 caatataaac cgacgcatgc agcggtggtc tttgacgcca agggaaaaac ctttcgtgat
     2401 gaactgtttg aacattacaa atcacatcgc ccgccaatgc cggacgatct gcgagcacaa
     2461 atcgaacccc tgcacgcgat ggttaaagcg atgggactgc cgctgctggc ggtttctggc
     2521 gtagaagcgg acgacgttat cggtactctg gcgcgcgaag ccgaaaaagc cgggcgtccg
     2581 gtgctgatca gcactggcga taaagatatg gcgcagctgg tgacgccaaa tattacgctt
     2641 atcaacacca tgacgaatac catcctcgga ccggaagagg tggtgaataa gtacggcgtg
     2701 ccgccagaac tgatcatcga tttcctggcg ctgatgggtg actcctccga taacattcct
     2761 ggcgtaccgg gcgtcggtga aaaaaccgcg caggcattgc tgcaaggtct tggcgggctg
     2821 gatacgctgt atgccgagcc agaaaaaatt gctgggttga gcttccgtgg cgcgaaaaca
     2881 atggcagcga agctcgagca aaacaaagaa gttgcttatc tctcatacca gctggcgacg
     2941 attaaaaccg acgttgaact ggagctgacc tgtgaacaac tggaagtgca gcaaccggca
     3001 gcggaagagt tgttggggct gttcaaaaag tatgagttca aacgctggac tgctgatgtc
     3061 gaagcgggca aatggttaca ggccaaaggg gcaaaaccag ccgcgaagcc gcaggaaacc
     3121 agtgttgcag acgaagcgcc agaagtgacg gcaacggtga tttcttatga caactacgtc
     3181 accatccttg atgaagaaac actgaaagcg tggattgcga agctggaaaa agcgccggta
     3241 tttgcatttg ataccgaaac cgacagcctt gataacatct ctgctaacct ggtcgggctt
     3301 tcttttgcta tcgagccagg cgtagcggca tatattccgg ttgctcatga ttatcttgat
     3361 gcgcccgatc aaatctctcg cgagcgtgca ctcgagttgc taaaaccgct gctggaagat
     3421 gaaaaggcgc tgaaggtcgg gcaaaacctg aaatacgatc gcggtattct ggcgaattac
     3481 ggcattgagc tgcgtgggat tgcgtttgat accatgctgg agtcctacat tctcaatagc
     3541 gttgccgggc gtcacgatat ggacagcctc gcggaacgtt ggttgaagca caaaactatc
     3601 acttttgaag agattgcggg taaaggcaaa aatcaactga cctttaacca gattgccctc
     3661 gaagaggccg gacgttacgc cgccgaagat gcggatgtca ccttgcagtt gcatctgaaa
     3721 atgtggccag atctgcaaaa acacaaaggg ccgttgaacg tcttcgagaa tatcgaaatg
     3781 ccgctggtgc cggtgctttc acgcattgaa cgtaacggtg tgaagatcga tccgaaagtg
     3841 ctgcacaatc attctgaaga gctcaccctt cgtctggctg agctggaaaa gaaagcgcat
     3901 gaaattgcag gtgaggaatt taacctttct tccaccaagc agttacaaac cattctgttt
     3961 gaaaaacagg gcattaaacc gctgaagaaa acgccgggtg gcgcgccgtc aacgtcggaa
     4021 gaggtactgg aagaactggc gctggactat ccgttgccaa aagtgattct ggagtatcgt
     4081 ggtctggcga agctgaaatc gacctacacc gacaagctgc cgctgatgat caacccgaaa
     4141 accgggcgtg tgcatacctc ttatcaccag gcagtaactg caacgggacg tttatcgtca
     4201 accgatccta acctgcaaaa cattccggtg cgtaacgaag aaggtcgtcg tatccgccag
     4261 gcgtttattg cgccagagga ttatgtgatt gtctcagcgg actactcgca gattgaactg
     4321 cgcattatgg cgcatctttc gcgtgacaaa ggcttgctga ccgcattcgc ggaaggaaaa
     4381 gatatccacc gggcaacggc ggcagaagtg tttggtttgc cactggaaac cgtcaccagc
     4441 gagcaacgcc gtagcgcgaa agcgatcaac tttggtctga tttatggcat gagtgctttc
     4501 ggtctggcgc ggcaattgaa cattccacgt aaagaagcgc agaagtacat ggacctttac
     4561 ttcgaacgct accctggcgt gctggagtat atggaacgca cccgtgctca ggcgaaagag
     4621 cagggctacg ttgaaacgct ggacggacgc cgtctgtatc tgccggatat caaatccagc
     4681 aatggtgctc gtcgtgcagc ggctgaacgt gcagccatta acgcgccaat gcagggaacc
     4741 gccgccgaca ttatcaaacg ggcgatgatt gccgttgatg cgtggttaca ggctgagcaa
     4801 ccgcgtgtac gtatgatcat gcaggtacac gatgaactgg tatttgaagt tcataaagat
     4861 gatgttgatg ccgtcgcgaa gcagattcat caactgatgg aaaactgtac ccgtctggat
     4921 gtgccgttgc tggtggaagt ggggagtggc gaaaactggg atcaggcgca ctaagattcg
     4981 cctgaacatg cccttttttc gtaagtaagc aacataagct gtcacgtttt gtgatggcta
     5041 ttagaaattc ctatgcaaca actgaaaaaa aattacaaaa agtgctttct gaactgaaca
     5101 aaaaagagta aagttagtcg cgtagggtac agaggtaaga tgttctatct ttcagacctt
     5161 ttacttcacg taatcggatt tggctgaata ttttagccgc cccagtcagt aatgactggg
     5221 gcgtttttta ttgggcgaaa gaaaagatcc gtaatgcctg atgcgctatg tttatcaggc
     5281 caacggtaga attgtaatct attgaattta cgggccggat acgccacatc cggcacaagc
     5341 attaaggcaa gaaaattatt cgccgtcctg cgtttcttct acaggctgca tctcgctaaa
     5401 ccaggtatcc agtttctgcc gcagcttgtc cacgccttgt ttcttcaacg aagaaaacgt
     5461 ttcaacctgc acatcaccgt taaacgccag tacagcttca cgcaccatat tcaattgcgc
     5521 tttacgtgcg ccgcttgcca gtttgtccgc tttggtcagc agcaccagaa cggcgatatt
     5581 gctgtctacc gcccactcaa tcatctgctg atccaaatct ttcagcggat ggcgaatatc
     5641 cattagcacc accagacctt gcaggctctg acgtttttcg aggtattcgc cgagcgcacg
     5701 ctgccatttg cgcttcatct cttccgggac ttccgcataa ccgtacccag gcaagtcaac
     5761 cagacgcttg ccgtcagcca cttcaaacag gttgataagc tgggtgcgcc ctggggtttt
     5821 tgaggtacga gccaggcttt tctggttagt cagcgtgttc agcgcgctgg atttacctgc
     5881 gttggaacgg cctgcaaaag ccacttcaat tccggtatcg gaaggtaggt ggcgaatatc
     5941 aggcgcactc atcacaaaat gcgtctgttg ataattcaaa ttagtcaaag cggtcgtctc
     6001 cgtcagtcaa agctttgtgg cgattatacc tgaaccataa taaaaggcgg atttttcggc
     6061 gtgagcgttg taagtaaaag ccatacgctt tgtgagacat tgccgatagt ctttatgcga
     6121 aatagcagag aaaattctgc gatgcatgtc aaataagcta tataaatcag tgaattgctt
     6181 ttatataaaa ctctgaaaat gtgcaaatac tgatggcggt tgattgtttg tttaaagcaa
     6241 aggcgtaaag tagcacccat agagcgagga cgctaacagg aacaatgact caggatgagg
     6301 gtcaggagcg ccaggaggcg aagacagagg attgtcagga agacaaacgt ccggagacgt
     6361 aattaaacgg aaatggaatc aacacggatt gttccggcta aaggaaaaac agggtgtgtt
     6421 ggcggcctgc aaggattgta agacccgtta agggttatga gtcaggaaaa aaggcgacag
     6481 agtaatctgt cgcctttttt ctttgcttgc tttctgttag attccgccgc aaatctatac
     6541 tgaataaaac ggctaaaaga cgaaccatta tgaaaccatc atcttcaaac tcacgcagca
     6601 aaggtcatgc aaaagcgcgt cgaaaaacac gcgaggagct ggatcaggaa gctcgtgatc
     6661 gcaagcgtca gaaaaaacgt cgtggtcatg cgccgggcag ccgcgcagcg ggcggaaaca
     6721 ccacgtcagg cagcaaaggc cagaacgcac caaaagatcc acgtattggc agtaaaaccc
     6781 ctattccatt gggcgtggct gaaaaagtca ccaaacagca caaaccgaag agtgagaaac
     6841 ctatgctttc accgcaggcg gagttggaat tactggaaac ggatgagcgt ctggatgcgc
     6901 tgctggaacg tctggaagca ggcgaaaccc tgagcgccga agagcaatcc tgggtggatg
     6961 ctaaactgga tcgtattgat gagttgatgc agaaactcgg cctctcttat gacgatgacg
     7021 aagaagagga agaagacgag aagcaagaag acatgatgcg tctgctgcgg ggtaactaac
     7081 ggattgccac cgtgggcctt cccgttctgc tgataaccct tccggttata tgttatctgg
     7141 tgtggttatt cgttaaacta cagcggttgt cgcggcgaca aaagtggctg cgcaaccggc
     7201 tgatgacccg aaacgggcat ccgccggtac gccgtagccg ccagagacgc catcggaagg
     7261 agtgagcatg tctgtacagc aaatcgactg ggatctggcc ctgatccaga aatataacta
     7321 ttccgggcca cgatacacct cgtacccgac cgcgctggag ttttcagaag acttcggcga
     7381 acaggcgttt ttacaagccg tggcgcgcta tcctgagcgt ccattatctc tctacgtaca
     7441 tatcccgttc tgccataagc tttgttactt ctgcggttgc aataagattg ttactcgcca
     7501 gcagcacaag gccgatcagt atctggacgc gctggagcaa gaaatcgtcc atcgtgcacc
     7561 gctgttcgcc gggcgtcacg tcagccaatt gcactggggc ggcggaacgc cgacgtatct
     7621 gaataaagcg caaatcagcc gcctgatgaa gctgctgcgc gaaaacttcc agttcaatgc
     7681 cgatgcggag atttcgatcg aagtcgatcc gcgggaaatc gaactggatg tactcgatca
     7741 tttacgcgcc gaaggcttta atcgcctgag catgggcgtg caggacttca acaaagaagt
     7801 gcaacgtctg gttaaccgcg agcaggatga agagttcatc tttgcactgc ttaaccatgc
     7861 gcgtgagatt ggttttacct ccaccaacat cgacctgatt tacggcctgc cgaaacagac
     7921 gccggagagt ttcgccttta ccctgaaacg tgtggcggag ctgaaccccg atcgtctgag
     7981 tgtctttaac tacgcgcatc tgccgaccat ttttgctgct cagcgcaaaa tcaaagatgc
     8041 tgacctgccg agtccgcagc aaaaactcga tatcctgcag gaaaccatcg ccttcctgac
     8101 gcaatcgggc tatcagttta tcggtatgga tcactttgcc cgtccggatg acgagctggc
     8161 ggtggcccag cgtgaaggcg tgctgcatcg taacttccag ggctacacca ctcagggcga
     8221 taccgatctg ctggggatgg gcgtttccgc catcagcatg attggcgact gctacgcgca
     8281 gaaccagaaa gagttgaagc agtactatca gcaagtggat gaacaaggca atgctctgtg
     8341 gcgtggtatt gcgctaacgc gtgatgactg tattcgccgc gatgtgatta agtcgctcat
     8401 ctgcaacttc cgtctggatt acgcccctat tgagaaacag tgggatttgc acttcgctga
     8461 ttactttgcg gaagatctca agctgctcgc cccgttagca aaagatgggc tggtggatgt
     8521 ggatgagaag ggaatacagg tgacggcgaa aggtcgcttg ctgatccgca acatttgcat
     8581 gtgctttgat acctatctgc gccagaaagc gcggatgcag cagttctcac gggtgattta
     8641 aatagtgaat ggcgcttcgt ttacaaagta gaaaacgaaa gcgccatcaa tgtgctgaga
     8701 acaagattgc ctgatgcgct acgcttatca ggcctacaaa acctgttgaa aatattgctt
     8761 ttgtaggccg gataaggcgt tcacgccgca tccggcatga acaaagagca cttcatcagc
     8821 aatctgaaac tcaactaaac agcccaatca ttgcggcaca cagcacggca gcaacggcag
     8881 tttgcggtgt gtggctgtca actgcgccac tcgataccag attaattatt gattccagca
     8941 taatgactct ccccgttttc cgggcaagat catactgaac ttatcggaac agtaaagcgt
     9001 aaaataccag caatttgcgc tcaataatca atctttacac acaagctgtg aatcactcca
     9061 tccccagctc ttttaactta cgcgtcaggg tgttgcggcc ccagccgagt agccgcgccg
     9121 cttcctgttt atgcccctgc gtatgtcgta acgcggtcgt cagtaacgtc cgctccagct
     9181 ctggctgcgc ttcggaaagc agattttgat gaccggaacg cagcgctctg tctgcccact
     9241 gcgcaagaag cgttgcccag ctgtccggtt gcatttgcga agtactctcc gcaaccgttg
     9301 attcaaacag ttcgccgggc aaatcctgaa tcaacacttc ctgcccggcg gccatcaccg
     9361 ttagccagcg gcaggtgttt tccagctggc gcacgttgcc tggccacgcc agacgcgtca
     9421 gagcagcttc ggtttccgga tgcagtaact tcgcttctac gcccagttcg cgcgcggcaa
     9481 cctgtaaaaa atggcgcgcc agacggggaa tatcttcccg acgttcgcgc agcggcggca
     9541 gatgaacgcg gataacgttc aggcggtgga acagatcctc acggaactta ccttcctgca
     9601 ctcgctgttc gagattctgg tgagtggcag cgataatacg cacatccact ttcaccggcg
     9661 catagccgcc aacgcggtaa aactgaccgt ctgccagcac gcgcagcaaa cgcgtctgca
     9721 catccagcgg catatcacca atttcgtcga ggaataatgt accgccatcg gcctgttcaa
     9781 aacgcccctg acgaatggta ttcgcgccag taaacgcgcc tttctcgtgg ccaaacagtt
     9841 ctgattcgat caaatctttt gggatagctg ccatattcag cgcgataaac ggcgctttgg
     9901 cgcgcggact gtggcgatgc agggcatgag cgaccagttc tttaccggtg ccggattcgc
     9961 cgttaatcag cacgctaata gaagaacgcg aaagccgacc gataatacgg aacacgtcct
    10021 gcatggctgg cgcttcgccg atgatatcgg tcgttgggcc gttaagctga atattacgcg
    10081 gctgctgctg ttcctggtaa tgactgatag cgcgctcaac cagcgccact gcttcgtcga
    10141 tatcaaacgg tttgggcaga taatcaaacg ccccttgttg ataggcgctg acggcagcat
    10201 ccagatcgga atgtgcggtc ataatgatga ccggaagcat tggatggcgc tgtttaatct
    10261 gcttgagcag cgccagcccg tccattcccg gcatacggat atctgaaagc agcacatccg
    10321 gcgttttgct cgccagcgcc tccagtactt ccgcgccgtt ctcaaatgtc gtacaggtta
    10381 aacccgctcc agcgagcgca cgttcaagca cccaacggat ggaactatcg tcatcgacta
    10441 cccagactat ccctcgttgc ataaacgtca cctttatttc ctgataggca ggtaaaccga
    10501 gaactcggta tgacctggcc aactggtaaa ttcaattttg cctgaatgct gatcaatcaa
    10561 attacgggcg atggataagc caagcccggt gccaccttcg cggccgctga ccatcgggta
    10621 aaacagcgta tcctgcaaat gaggcggaat gcccggcccg ttatcttcca catcaatccg
    10681 cgccgccagc cggtagcgct cgccgtgtaa ggtcagttga aacgcggtgc gggtacgcag
    10741 aatgatttca ccgccttccg gccccagcgc ctgtagcgca ttgcgcacaa tattcagcaa
    10801 gacctgttca atttgatccg ggtcgtgcgc cagttccggc aggctggggt cgtaatcacg
    10861 aatcaaccgc acgttgtccg gcagttccat cgacaccagc gtcaccacgc gttcagctac
    10921 tttgtgaata ctttcggtaa cgcgcgtacc gggcagctgc ggccccaaca gacggtcgac
    10981 cagatttcgc agccggtccg cctgttcgat aatcactttg gtatattcga gtagtgatgg
    11041 gtcaggtaac gctttgctga gcagctgcgc cgcgccacgt aaacctccaa gcggattttt
    11101 aatctcatgt gccaggccgc gcactaaatc acgggcagca acctgctggg cgtgctgtag
    11161 ctgttcctga cttaagcggc gctggttatc catcggagcc atctccagca ggatcatgcc
    11221 gtccggcata cgctgggccg tcacagaaag gatatgcgag cgcccgtcga tgaccagcgt
    11281 cacttcgtta tcggtaaaac cttgccccgc ctccagactt tcttgcatca gctcgatatt
    11341 taatgagaag tagctcaaca gttccggtaa cggtgtacca aacaatttgc gggagctttg
    11401 ggcgagcagt tgttgcgcgg cggggttggc gtaatggatc gccagattgt catcaattaa
    11461 caaaatactg ttaatcagcg agttgaggat ctgcccagca tcgggctgcg tgcctgttgc
    11521 cataaagcag tctcctgaac aggttgcacc attttagtgc attatagctt tttacggata
    11581 aaaagcgcga agcatcagag aattgacgga gaaaaaagcc catgcagaga tgggctgaaa
    11641 gtttccacgg caactaaatc ccggcgttgt tgcgccgggt agtacaactt caactattag
    11701 acgctgtagt acagctcaaa ctctaccgga tgcggagtca tacgcacgcg gtcatcttct
    11761 tcgcgacgta gagcgatgta cgcatcaatt gcttcgtcag tgaacacgcc accggctttc
    11821 aggaactcgc ggtccagatc cagttcgttc agtgcttctt ccagagagcc tgcaacctgt
    11881 gggatctctt tcgcttcttc tggcggcagg tcatacaggt ttttgtccat ggcttcgccc
    11941 ggatggatct tgttcttgat accatcaaga ccggccatca gcagggcagc aaagcacagg
    12001 tacgggttag ctgccggatc cgggaaacgt acttcgatac gacgtgcttt cggagaagaa
    12061 accaccggaa tacggataga cgcagaacgg ttacgcgcag agtaagccag cattaccggt
    12121 gcttcatagc ccgggaccag acgcttataa gagttggtgg tcgggtttgc cagggcgtta
    12181 atcgctttag cgtgtttgat tacgccgcca atgtagtaca gcgcctgctc agacagacct
    12241 gcgtatttgt cgcctgcgaa caggttaacg ccgtttttag acagagacat gtggcagtgc
    12301 ataccggagc cgttatcacc gaacatcggt tttggcataa aggtcgcggt tttaccgaag
    12361 cggtgcgcta cgttgtgcac aacatatttg tagatctgaa tttcgtcagc ttttttggtc
    12421 atagtattga agcgggtagc cacttcgttc tgaccagcag tcgctacttc gtggtgatgg
    12481 gcttcaacca ccagacccat ctgttccatc accagacaca tttcagaacg aatatcctga
    12541 gccgagtcta ccggtggaac cgggaagtaa ccgcctttca ctgccggacg gtgacctttg
    12601 ttaccacctt cgtattgggt ggaggagttc catgcgcctt cgatatcgtc gatagcaacg
    12661 tgggaaccgg agatagatga tccgaaacgg atgtcatcga acaggaagaa ttcaggttct
    12721 ggcccgaaca gtacggtgtc ggcaatgcca gtggaacgca ggtaatcttc ggcgcgcttc
    12781 gcaatggagc gcgggtcacg gtcatagcct tgcagggtgc caggttcaag gatgtcgcaa
    12841 cggataatca gggtggagtc ggcgaagaac gggtcaatca ctgcggtgga tgcgtctggc
    12901 atcagcacca tgtcggactc gttaatgcct ttccagccgc caatcgagga gccgtcaaac
    12961 attttgcctt cttcgaagaa ttcagcattc acctgatgag cagggatagt gacgtgctgt
    13021 tctttacctt tagtatcggt gaagcgcaaa tcaacaaact tcacttcgtg ctcgttcagc
    13081 atcgtcagta cgtgttcagc ggacatactt taactctcct ggattggtca tggtcgtcgt
    13141 ggtaacgaaa tctgcaatta ttttggccgt gtcgccgtaa aaaagataaa gcgaaatctg
    13201 tgccaacttt taaattgccc ctaaaaggcg ttatcatgcg caccatcgtg caaaagggct
    13261 gcaccacgat gtgaatgttg caccaatata gtgcttcaat ggaaacatta agcaccatgt
    13321 tggtgcaatg acctttggat aacccttttt atgctccgtg aaagcgatca caaagggact
    13381 ctgcaatact tgtttgcgga ggatgtttgt gatcctgttt tgtagtgcga ttaatccgtg
    13441 tacaataacg cgctatttct aatgcctgag gcaaagttgt gatcgaaaaa ttgcgtaata
    13501 tcgccatcat cgcgcacgta gaccatggta aaaccaccct ggtagacaag ctgctccaac
    13561 aatccggtac gttcgactct cgtgccgaaa cccaagagcg cgtgatggac tccaacgatt
    13621 tggagaaaga gcgtgggatt accatcctcg cgaaaaacac cgctatcaaa tggaatgatt
    13681 accgtatcaa catcgttgat accccggggc acgccgactt cggtggtgaa gttgaacgtg
    13741 taatgtccat ggtagactca gtgctgctgg tggttgacgc atttgacggc ccgatgccgc
    13801 aaacgcgctt cgtaaccaaa aaagcgtttg cttacggcct gaagccgatt gttgttatca
    13861 acaaagttga ccgccctggc gcgcgtcctg attgggttgt ggatcaggta ttcgatctgt
    13921 tcgttaacct cgacgcgacc gacgagcagc tggacttccc gatcgtttac gcttctgcgc
    13981 tgaacggtat cgcgggtctg gaccacgaag atatggcgga agacatgacc ccgctgtacc
    14041 aggcgattgt tgaccacgtt cctgcgccgg acgttgacct tgacggtccg ttccagatgc
    14101 agatttctca gctcgattac aacagctatg ttggcgttat cggcattggc cgcatcaagc
    14161 gcggtaaagt gaagccgaac cagcaggtca ctatcatcga tagcgaaggc aaaacccgca
    14221 acgcgaaagt cggtaaagtg ctgggccacc tcggtctgga acgtatcgaa accgatctgg
    14281 cggaagctgg cgatatcgtg gcgatcacgg gccttggcga actgaacatt tctgacaccg
    14341 tttgcgacac gcaaaacgtt gaagcgctgc cggcactctc cgttgatgag ccgaccgttt
    14401 ctatgttctt ctgcgttaac acctcgccgt tctgcggtaa agaaggtaag ttcgtaacgt
    14461 ctcgtcagat cctggatcgt ctgaacaaag aactggtaca caacgttgcg ctgcgcgtag
    14521 aagaaaccga agacgccgat gcgttccgcg tttctggtcg tggcgaactg cacctgtctg
    14581 ttctgatcga aaacatgcgt cgtgaaggtt tcgaactggc ggtatcccgt ccaaaagtaa
    14641 tcttccgtga aatcgacggt cgtaaacaag agccgtatga aaacgtgact ctggacgttg
    14701 aagaacagca tcagggttct gtaatgcagg cgctgggcga acgtaaaggc gacctgaaaa
    14761 acatgaatcc agacggtaaa ggccgcgtac gtctcgacta cgtgatccca agccgtggtc
    14821 tgattggctt ccgttctgag ttcatgacca tgacttccgg tactggtctg ctgtactcca
    14881 ccttcagcca ctacgacgac gtacgtccgg gtgaagtggg tcagcgtcag aacggcgtac
    14941 tgatctctaa cggtcagggt aaagcggtcg cgttcgcact gttcggtctg caggaccgcg
    15001 gtaagctgtt cctcggtcac ggtgcagaag tttacgaagg tcagattatc ggtattcaca
    15061 gccgctctaa cgacctgact gtaaactgcc tgaccggtaa gaaactgacc aacatgcgtg
    15121 cttccggtac tgacgaagct gttgttctgg ttccgcctat ccgcatgact ctggaacaag
    15181 ctctggagtt catcgatgat gacgaactgg tagaagtgac tccgacctct atccgtattc
    15241 gtaaacgtca cctgacggaa aacgatcgtc gtcgcgccaa ccgcgcaccg aaagacgatt
    15301 aatttcgttt tttagttata aaaaacctgc catcgct
//
LOCUS       DAETQN010000105        15084 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.105, whole genome shotgun sequence.
ACCESSION   DAETQN010000105 DAETQN010000000
VERSION     DAETQN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.105"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..781
                     /locus_tag="K0W68_003215"
     CDS             <1..781
                     /locus_tag="K0W68_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074700.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHN
                     LFDHYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVP
                     CQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(984..1337)
                     /locus_tag="K0W68_003216"
     CDS             complement(984..1337)
                     /locus_tag="K0W68_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBI3074701.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1475..3121)
                     /gene="groL"
                     /locus_tag="K0W68_003217"
     CDS             complement(1475..3121)
                     /gene="groL"
                     /locus_tag="K0W68_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBI3074702.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3165..3458)
                     /locus_tag="K0W68_003218"
     CDS             complement(3165..3458)
                     /locus_tag="K0W68_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBI3074703.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3734..4990
                     /gene="yjeH"
                     /locus_tag="K0W68_003219"
     CDS             3734..4990
                     /gene="yjeH"
                     /locus_tag="K0W68_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBI3074704.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="K0W68_003220"
     CDS             complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="K0W68_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBI3074705.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5819..7255
                     /gene="aspA"
                     /locus_tag="K0W68_003221"
     CDS             5819..7255
                     /gene="aspA"
                     /locus_tag="K0W68_003221"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBI3074706.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7373..8674
                     /gene="dcuA"
                     /locus_tag="K0W68_003222"
     CDS             7373..8674
                     /gene="dcuA"
                     /locus_tag="K0W68_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBI3074707.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8790..9128
                     /gene="cutA"
                     /locus_tag="K0W68_003223"
     CDS             8790..9128
                     /gene="cutA"
                     /locus_tag="K0W68_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBI3074708.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9104..10801
                     /gene="dsbD"
                     /locus_tag="K0W68_003224"
     CDS             9104..10801
                     /gene="dsbD"
                     /locus_tag="K0W68_003224"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBI3074709.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10838..11413
                     /locus_tag="K0W68_003225"
     CDS             10838..11413
                     /locus_tag="K0W68_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3074710.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11520..11595
                     /locus_tag="K0W68_003226"
     tRNA            11520..11595
                     /locus_tag="K0W68_003226"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11553..11555,aa:Phe,seq:gaa)
     gene            12212..13749
                     /gene="cadC"
                     /locus_tag="K0W68_003227"
                     /pseudo
     CDS             12212..13749
                     /gene="cadC"
                     /locus_tag="K0W68_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
     gene            14114..>15084
                     /gene="cadB"
                     /locus_tag="K0W68_003228"
     CDS             14114..>15084
                     /gene="cadB"
                     /locus_tag="K0W68_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HBI3074711.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3708 a         3764 c         3616 g         3996 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
     1021 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
     1081 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
     1141 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
     1201 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
     1261 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
     1321 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
     1381 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
     1441 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
     1501 tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa
     1561 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
     1621 gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
     1681 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
     1741 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
     1801 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
     1861 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
     1921 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
     1981 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt
     2041 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
     2101 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
     2161 tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga
     2221 tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
     2281 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
     2341 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
     2401 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
     2461 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
     2521 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
     2581 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
     2641 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
     2701 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt
     2761 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag
     2821 tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg
     2881 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
     2941 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
     3001 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
     3061 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
     3121 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
     3181 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
     3241 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
     3301 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
     3361 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
     3421 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
     3481 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
     3541 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
     3601 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
     3661 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
     3721 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
     3781 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
     3841 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
     3901 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt
     3961 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
     4021 tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
     4081 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
     4141 cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
     4201 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
     4261 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
     4321 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
     4381 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
     4441 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
     4501 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
     4561 tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
     4621 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
     4681 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt
     4741 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
     4801 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
     4861 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
     4921 gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
     4981 aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt
     5041 cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
     5101 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
     5161 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
     5221 gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag
     5281 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
     5341 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc agcaataccc
     5401 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
     5461 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
     5521 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
     5581 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
     5641 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
     5701 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
     5761 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
     5821 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
     5881 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
     5941 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
     6001 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
     6061 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
     6121 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
     6181 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
     6241 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
     6301 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
     6361 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
     6421 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
     6481 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
     6541 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
     6601 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
     6661 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
     6721 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
     6781 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
     6841 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
     6901 gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa
     6961 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
     7021 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
     7081 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
     7141 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
     7201 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
     7261 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     7321 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
     7381 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
     7441 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
     7501 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
     7561 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
     7621 cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg
     7681 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
     7741 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
     7801 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
     7861 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
     7921 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
     7981 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
     8041 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
     8101 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
     8161 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
     8221 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
     8281 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
     8341 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
     8401 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
     8461 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
     8521 ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa
     8581 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
     8641 cttcggcttc gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc
     8701 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
     8761 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
     8821 ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag
     8881 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
     8941 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
     9001 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
     9061 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
     9121 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
     9181 acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
     9241 tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
     9301 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
     9361 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
     9421 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
     9481 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
     9541 agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc
     9601 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
     9661 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
     9721 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
     9781 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
     9841 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
     9901 actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc
     9961 acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac
    10021 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    10081 cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    10141 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    10201 ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg
    10261 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    10321 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    10381 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    10441 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    10501 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt
    10561 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    10621 tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    10681 ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    10741 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    10801 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    10861 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    10921 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    10981 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    11041 gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    11101 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    11161 cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    11221 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    11281 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    11341 cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    11401 tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    11461 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    11521 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    11581 cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag
    11641 gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc
    11701 cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat
    11761 agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt
    11821 catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat
    11881 tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat
    11941 ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct
    12001 ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc
    12061 tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac
    12121 caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt
    12181 gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga
    12241 atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga
    12301 gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag
    12361 ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca
    12421 gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat
    12481 cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga
    12541 agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac
    12601 aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc
    12661 agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt
    12721 atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg
    12781 cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc
    12841 tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca
    12901 cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg
    12961 agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa
    13021 caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa
    13081 cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta
    13141 tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg
    13201 cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc
    13261 gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca
    13321 gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac
    13381 cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg
    13441 ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa
    13501 ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg
    13561 ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct
    13621 gatgcatatc tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa
    13681 aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct
    13741 tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta
    13801 tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt tattacataa
    13861 atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc ttttcatgat
    13921 atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta cagaaacttt
    13981 tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc ttatcgccag
    14041 tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc aaaaatgaaa
    14101 ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta ccggtgttgt
    14161 tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag caagtatcgg
    14221 tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc tggcgtatgt
    14281 atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt atgccggaga
    14341 aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta actggattgg
    14401 taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc cagtattaaa
    14461 tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta cctttgtaaa
    14521 tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc tggttcttat
    14581 tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa cttatgcagc
    14641 taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta ttctgctcta
    14701 cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg ttaaaaaccc
    14761 gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta ttgtttacat
    14821 cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg cttccggtgc
    14881 tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc tggtttctgc
    14941 attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg taggccaggc
    15001 aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag tcgacagcaa
    15061 cggtattccg aaaaaaggtc tgct
//
LOCUS       DAETQN010000106        14965 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.106, whole genome shotgun sequence.
ACCESSION   DAETQN010000106 DAETQN010000000
VERSION     DAETQN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.106"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="K0W68_003229"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="K0W68_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBI3074712.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="K0W68_003230"
     CDS             complement(1608..1931)
                     /locus_tag="K0W68_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBI3074713.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="K0W68_003231"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="K0W68_003231"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBI3074714.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="K0W68_003232"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="K0W68_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBI3074715.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="K0W68_003233"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="K0W68_003233"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBI3074716.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="K0W68_003234"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="K0W68_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBI3074717.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="K0W68_003235"
     CDS             7531..8568
                     /locus_tag="K0W68_003235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBI3074718.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="K0W68_003236"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="K0W68_003236"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBI3074719.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="K0W68_003237"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="K0W68_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBI3074720.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="K0W68_003238"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="K0W68_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBI3074721.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="K0W68_003239"
     CDS             10739..11620
                     /locus_tag="K0W68_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3074722.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="K0W68_003240"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="K0W68_003240"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBI3074723.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="K0W68_003241"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="K0W68_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBI3074724.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14965)
                     /locus_tag="K0W68_003242"
     CDS             complement(14017..>14965)
                     /locus_tag="K0W68_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBI3074725.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     RCAMMRKESRGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3612 a         3785 c         3921 g         3647 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgccc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat tggcaacccg
    14941 actgaggtgt actggcaata gcgga
//
LOCUS       DAETQN010000107        14746 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.107, whole genome shotgun sequence.
ACCESSION   DAETQN010000107 DAETQN010000000
VERSION     DAETQN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14746
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.107"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            234..1898
                     /gene="tssM"
                     /locus_tag="K0W68_003243"
                     /pseudo
     CDS             234..1898
                     /gene="tssM"
                     /locus_tag="K0W68_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354198.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1909..3261
                     /locus_tag="K0W68_003244"
     CDS             1909..3261
                     /locus_tag="K0W68_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HBI3074726.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPIESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            3285..3767
                     /locus_tag="K0W68_003245"
     CDS             3285..3767
                     /locus_tag="K0W68_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBI3074727.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            3811..4756
                     /locus_tag="K0W68_003246"
                     /pseudo
     CDS             3811..4756
                     /locus_tag="K0W68_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4766..5245
                     /locus_tag="K0W68_003247"
     CDS             4766..5245
                     /locus_tag="K0W68_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074728.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(5382..6167)
                     /locus_tag="K0W68_003248"
     CDS             complement(5382..6167)
                     /locus_tag="K0W68_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074729.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(6497..6573)
                     /locus_tag="K0W68_003249"
     tRNA            complement(6497..6573)
                     /locus_tag="K0W68_003249"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6537..6539),aa:Asp,seq:gtc)
     gene            complement(6706..7437)
                     /gene="dnaQ"
                     /locus_tag="K0W68_003250"
     CDS             complement(6706..7437)
                     /gene="dnaQ"
                     /locus_tag="K0W68_003250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBI3074730.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            7502..7969
                     /gene="rnhA"
                     /locus_tag="K0W68_003251"
     CDS             7502..7969
                     /gene="rnhA"
                     /locus_tag="K0W68_003251"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBI3074731.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(7966..8688)
                     /locus_tag="K0W68_003252"
     CDS             complement(7966..8688)
                     /locus_tag="K0W68_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI3074732.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            8722..9477
                     /gene="gloB"
                     /locus_tag="K0W68_003253"
     CDS             8722..9477
                     /gene="gloB"
                     /locus_tag="K0W68_003253"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBI3074733.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            9549..10907
                     /gene="mltD"
                     /locus_tag="K0W68_003254"
     CDS             9549..10907
                     /gene="mltD"
                     /locus_tag="K0W68_003254"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBI3074734.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(10956..11552)
                     /locus_tag="K0W68_003255"
                     /pseudo
     CDS             complement(10956..11552)
                     /locus_tag="K0W68_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(11583..12383)
                     /locus_tag="K0W68_003256"
     CDS             complement(11583..12383)
                     /locus_tag="K0W68_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBI3074735.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            12624..13538
                     /locus_tag="K0W68_003257"
     CDS             12624..13538
                     /locus_tag="K0W68_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3074736.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(13535..14338)
                     /gene="dkgB"
                     /locus_tag="K0W68_003258"
     CDS             complement(13535..14338)
                     /gene="dkgB"
                     /locus_tag="K0W68_003258"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBI3074737.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(14501..14577)
                     /locus_tag="K0W68_003259"
     tRNA            complement(14501..14577)
                     /locus_tag="K0W68_003259"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14541..14543),aa:Asp,seq:gtc)
BASE COUNT         4064 a         3480 c         3498 g         3704 t
ORIGIN      
        1 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
       61 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
      121 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
      181 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
      241 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
      301 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
      361 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
      421 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
      481 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
      541 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
      601 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
      661 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
      721 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
      781 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
      841 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
      901 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
      961 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
     1021 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
     1081 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
     1141 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
     1201 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
     1261 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
     1321 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
     1381 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
     1441 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
     1501 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
     1561 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
     1621 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
     1681 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
     1741 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
     1801 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
     1861 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
     1921 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
     1981 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
     2041 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
     2101 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
     2161 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
     2221 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
     2281 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
     2341 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
     2401 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
     2461 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
     2521 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
     2581 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
     2641 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
     2701 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
     2761 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
     2821 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
     2881 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
     2941 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
     3001 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
     3061 caggcggagc ggggactggg gcaggaaacc cccattgagt ctctgttgac gcagtaccat
     3121 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
     3181 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
     3241 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
     3301 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
     3361 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
     3421 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
     3481 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
     3541 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
     3601 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
     3661 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
     3721 tagccggtac gaatgcaatg agtaaatggc aggatcgtgt acagtaactt actgtaataa
     3781 cataataaat tactattgcg gagagataaa atggctggtt taaggaataa caacaacaca
     3841 cagaatgcac agtgggctga ttatgtgggt gacattcttc ggggggctca gccgataaac
     3901 cagctagtac ctcagcaccc ttatcttaac gacgtcccac tcattgatga gttaaggcat
     3961 cagaacactc atcatgttat tctcttgaca cttgatgttg caaaaaaaaa aaaaaaaaaa
     4021 aaaaaaaaaa aaaaaaaaaa tactcagccc aataacatca tttgattaca ttcattttat
     4081 cactacacat ccttcaggta taaaagatac actcgcctgg ttagttaacg cgagtaaatt
     4141 aatgactgag tttgatgaca atgggaaaat tatatttaac attaatgcct taaagtatac
     4201 aaaagcaagt tactttgaaa tacttggaga gaaatatata aaaatcacaa catccagccc
     4261 ctggctttta gaaaagctcg gtaaatatat tttctcttca cgagcgcctc aagttctgga
     4321 actcgctatt ggctggcgag gagctttatc tgaatcaata aaaggagtta agttctgtat
     4381 ctggttttca gtagcctgga gaaccataga atttattatg agttcagagc gagatctggt
     4441 taattttctt ggcgattttt caatggatgt ggccaaagca gttatagccg gtggtgttgc
     4501 aaccgctatt ggaagcctgg cttcttttgc ctgtgttagc tttggctttc cagtaattct
     4561 tgtcggagga gcaattttac tgacagggat agtgtgtaca gttgttttaa atgaaatcga
     4621 tgctcaatgc catttatcag aaaaattaaa atatgcaatt agagatggac taaaacggca
     4681 acaggaactt gataaatgga aaagggaaaa catgactcca tttatgtatg ttcttaacac
     4741 tccacccgtg atatgaggat taaccatgcg tttacatgtt aaattaaaag agtttctttc
     4801 gatgtttttt atggccatat tatttttccc tgcattcaat gcttctttgt tttttaccgg
     4861 agttaaacct ctatattcaa taataaaatg ctccacggaa atattttatg attggagaat
     4921 gttgattctg tgctttggtt tcatgtcgtt ttccttttta aatattcatg taattttact
     4981 tacgataata aaattattct taatcaaaaa aacaaaggta gtcaatttcg ccacagacat
     5041 aacaatacag ctaactctta tatttttact aatagctatc gtaatagcac ctttaatagc
     5101 tccttttgtc acaggatatg tcaatgctaa ttatcacccc tgcggtaata atacgggaat
     5161 tttcccagga gcaatctata ttaaaaatgg catgaaatgt aataatgaat atatttctcg
     5221 caaggaagat tcagcagtta aataattaaa cattctaaaa caaacagttt ttaactccgg
     5281 attccatgtc ctttcttgca ctgtctttgt caagaaaaat ttcttatgca aagaattttg
     5341 tattatttcc gccagaaatt cctggcggca gatataattt attattgata gcggttaacc
     5401 aaatctttta ccatcgcttt ataggtcttt tgcttaagtt gcatgatttg ctgctctgtc
     5461 atatcagtcg tactggcttc attcttgtcg ggcaatggtg gaagcacttt atattcataa
     5521 ttcaccggcg accaggcagc ccactcacct gtagctacat ccaccagcgt gaatcgtacc
     5581 agatagcgca gtgacatcgt gtcagtcaat ttttgatttt tataatctga ccaaactatt
     5641 gatttcaatc cagagtcata ttttcctgtc tgcaacgtat cctggaaaac aataatcgct
     5701 ttctgatgcc cttttgcagc cacaaaacgc agtgcctgca tccagttcat attctcagca
     5761 ctggcaacat cctcgttctc atttttatct ttgtttttgt tacaagtcag aggcttttgc
     5821 ctgtccggga taccagagaa tgtggaaaca gtataatatt ttcgcatctc ctcctgcata
     5881 atggtttccg gtgcgcggtt gccggattga acaagtatta caggagaatt aaggggaacg
     5941 cggaactcat ctccatcaag cgcagcttga atatcctctt cggatacggc cagagtggtt
     6001 tcattaccga ataaatcttt gtctgtcagt tgctggggag tgtttttatt aatattactt
     6061 tgtgtgactt gagctttttt cttcttttca tcaatagaag attcagagac acatcctgca
     6121 agcagcgaga ataatacaca tatacaacaa acccttctta aattcataat cggacactcc
     6181 ctcgcctgtc ataatcatgg taatgtttag ctaacgattt ttaattatat actccacttg
     6241 aatcagaaca aacaaaaata acagtatata taaattcatt aaatcaatag aaaaatgaaa
     6301 atcataacat tcaaatatat ttaaattgaa tgatttgata gtatgattaa atattaatga
     6361 tatggtctac catatgtgat taacacattt acagcataac aaaaccatag accgaagaaa
     6421 tagcgggatt taaatgcaaa aaaaccgcaa aattaaaaat cttgcggttc tctgaactca
     6481 ttttcatgag tgaatttggc ggaacggacg ggactcgaac ccgcgccccc ctgcgtgaca
     6541 ggcaggtatt ctaaccgact gaactaccgc tccgcgttgt gttccgttgg gaacgaggcg
     6601 aatattacgg attgcctcac ccttcgtcaa cggtttttct catcttttga atcgtttgct
     6661 gcaaaaatcg cccaagccgc tatttttagc gccttttaca ggtatttatg cccgccagag
     6721 gcagcttccg cccttcttct ccaccagatc aagacgggct tcatgagctg caagctcttc
     6781 atctgtcgca aaaacaacgc gtaacttact tgcctgacgt acaatgcgct gaattgttgc
     6841 ttcaccttgt tgctgctgtg tctctccttc catcgcaaaa gccatcgacg tttgaccacc
     6901 ggtcatcgcc agataaactt ccgcaaggat ctgtgcatcg agtaatgccc cgtgtagcgt
     6961 tcgtttactg ttatctattt cgtagcgggc acataacgca tcgaggctgt tgcgcttacc
     7021 aggaaacatt ttcctcgcca ccgcaaggct atcggtgacc ttacagaagg tattggtctt
     7081 cggaatatcg cgcttaagca acgaaaactc gtagtccata aagccgatat cgaacgctgc
     7141 gttatggatc accaactccg cgccgcggat atagtccatg aactcatcgg ctacttcggc
     7201 aaacgtgggc ttatcgagca aaaattcatc ggcaatacca tgtacgccaa aggcttccgg
     7261 atccaccagc cgatcgggtt tgagataaac atggaagtta ttgcccgtca ggcgacggtt
     7321 caccacttca acggcaccaa tctcaatgat cttgtggcct tcatagtgcg caccaatctg
     7381 gttcataccg gtggtttcgg tatcgagaac gatctggcgt gtaattgcag tgctcatagc
     7441 ggtcatttat gtcagactta tcgttttaca gttcgattca attacaggaa gtctaccaga
     7501 gatgcttaaa caggtagaaa ttttcaccga tggttcgtgt ctgggcaatc caggacctgg
     7561 gggttacggc gctattttac gctatcgcgg acgcgagaaa acctttagcg caggctacac
     7621 ccgcaccacc aacaaccgta tggagttgat ggccgctatt gtcgcgctgg aggcgttaaa
     7681 agaacattgc gaagtcattt tgagtaccga cagccagtat gtccgccagg gtatcaccca
     7741 gtggatccat aactggaaaa aacgtggctg gaaaaccgca gacaaaaaac cagtaaaaaa
     7801 tgtcgatctc tggcaacgtc ttgatgctgc cctagggcaa catcaaatca aatgggaatg
     7861 ggttaaaggc catgccggac acccggaaaa cgaacgctgt gatgaactgg ctcgtgctgc
     7921 ggcaatgaat cccacactgg aagatacggg ctaccaagtc gaagtttaag cttgtggttt
     7981 acgacattgc cgggtggctc caaccgcctg gcgaattcgt ggcttgtttt tactctgttt
     8041 catcgggttt agcgttaaag gaatagtccg tttccgggca acaataagtt gtaagcaacc
     8101 aagcgcagga atatgcgcat tcaatagttt tcctccgtgt ttgttccacg ggagaacgtg
     8161 gaaacggctg gcgtgcagca cttcaaaatt caacaaagag agccagtcca gttgccgcat
     8221 cagagtaaac atccggctgt tatagggcga ggtttttcgc aataccggca caagtttgcg
     8281 taatcccatt aaactaatgg gattgaagcc actaatcacc agccagccat catcaatcaa
     8341 tacccgatcg gtttcacgca ataaacgatg cggatcggtg caccacggca atgtatgtgc
     8401 cagtagacaa acatcaacgg atttatcggc aaaaggaaga tgaagtggat ccgcctgcac
     8461 ctggacgggc attccttgcg cagaaacatt cacttgatga gaaaccgcgc acgcttcgca
     8521 attgatttct gcgcttaaat tgccaatctt aagcagatga aaaccataca ttttagtgaa
     8581 ccacgggttg agctggcgct ccagcgcctt gcgataaagc tctccccagg gcaaatcgcc
     8641 ccagcgatca ggagccacga cagtttgagg gactcttgcc ggtttcatca caaccttccg
     8701 tttcacacta agaggtaatc tatgaatctt aacagtattc ccgcctttga tgacaattac
     8761 atctgggttt tgaatgatga agcaggtcgc tgcctgattg tcgatcccgg agacgcagag
     8821 ccagtattaa acgccattgc cgccaataac tggcaaccgg aggccatatt tctcacccac
     8881 catcatcacg atcacgttgg cggcgtaaaa gaactggtgg aaaagtttcc gcaaattgtg
     8941 gtgtatggtc cacaagagac acaagataag ggaacaacac aggtagtcaa agatggcgaa
     9001 actgccttcg ttttggggca tgaatttagt gtaattgcca caccgggtca cactttagga
     9061 catatctgtt acttcagtaa accttatcta ttttgcggcg acacgctgtt ttctggtggg
     9121 tgtggtaggt tgtttgaagg gacagcatca caaatgtatc aatcacttaa taagttaagt
     9181 gcgttacctg acgatacatt ggtatgttgt gctcatgaat atacgttatc aaatatgaag
     9241 tttgctttga gtattcttcc gcacgatttg tccataaatg attattatcg taaagttaag
     9301 gagttacggg caaaaaatca aataacacta cccgtaattc tgaaaaatga gcggcaaatt
     9361 aatgtttttt taagaacgga agatattgat ttaattaatg taattaatga agaaacatta
     9421 ttgcaacaac ctgaagagcg ttttgcatgg ttaaggtcaa agaaagatag gttctgataa
     9481 aactttcttg tcatcggctc cgttcgccgt tatgatcggt cgtcttttaa gcaactattg
     9541 acacacacat gaaggcaaaa gcgatattac tcgcctctgt cctgctcgtg ggttgccaga
     9601 gtaccggcaa cgttcaacag cacgcacaga gcctttctgc agctggtcaa ggggaagcag
     9661 caaagtttac aagtcaggca cgatggatgg acgatgggac gtctatcgcg ccagatggtg
     9721 acttgtgggc tttcattggc gacgagctaa agatgggaat tccggaaaat gaccggattc
     9781 gcgaacagaa acagaaatat ttacgcaata agagctatct ccacgatgta actttacggg
     9841 cagagccgta tatgtactgg atagcagggc aagttaaaaa acgtaacatg cctatggaac
     9901 tggtactact acccatagtg gagagcgctt ttgatcctca cgcaacgtct ggcgccaatg
     9961 ccgcaggcat ctggcagatc attccgagca cggggcgcaa ttatggtttg aaacagaccc
    10021 gcaattatga cgcgcgtcgc gatgttgttg cttcaacaac tgccgcgctg aatatgatgc
    10081 agcgtctgaa caaaatgttt gacggcgact ggcttctgac cgtagcggct tataacagcg
    10141 gcgaaggtcg ggtcatgaag gcaattaaaa cgaacaaagc gcgtgggaaa tccacggact
    10201 tctggtcgtt accgttgccg caggaaacga agcagtacgt gcctaaaatg ctggcattga
    10261 gtgatattct caaaaacagc aagcgttatg gcgtacgtct gccaacgacc gatgaaagcc
    10321 gtgctctggc gcgtgtgcac ctgagtagcc cggttgaaat ggcgaaggtt gcagatatgg
    10381 cggggatttc cgtcagcaag ctgaagacat tcaacgctgg cgtgaaaggc tccacgctgg
    10441 gcgcaagtgg gccgcagtac gtgatggtgc caaagaagca tgcagatcaa ctgcgtgaat
    10501 ctctcgcttc aggcgaaatt gctgctgtac agtcgacgct ggttgccgac aatacgccgc
    10561 ttaacagccg tgtttacacc gtacgctctg gcgacacgct ttcaagcatc gcttcacgtc
    10621 tcggcgtaag caccaaagat ttgcagcagt ggaacaaact gcgcggatct aagctgaagc
    10681 caggccaaag tttgacgatt ggtgcaggca gtagcgcaca gcggttggca aacaacagcg
    10741 atagcattac gtatcgtgtg cgcaaaggcg attcgctttc aagcattgct aaacgccacg
    10801 gcgtgaacat caaagatgtg atgcgctgga acagcgacac tgcgaatctg caaccaggcg
    10861 ataagctgac gttgtttgtg aaaaacaaca acatgccaga ttcctgacaa accagataat
    10921 aaaaaaggca ccgattcccc cggtgccttt tttatttatg ccgctttatg tgcttctacc
    10981 atgatgatat cactggtgaa agagccatca gtctgcaagg aaaaatacgt tctcacctct
    11041 gtcgatgcgc tctgttggta aatgcgaata gcgtctacta acgcttctgg cgtacgcatt
    11101 ctcacgaccc atgaagaaaa ttccagcggt aacttatctg taattaaatt atcaactatc
    11161 agattggctt cattgattaa cgtcaaccac gcaccgctgg cgtagtttcg tacgtgagag
    11221 gtatcgcgta atgcttctac cgcctgtaac cagatgtcgc gcactgggtg acccggagac
    11281 attacatcca tcacaatcag cctaccgcca ggtttcaata tcctattcac ttctcgcagt
    11341 gctgcaccaa catcatgcca gtgatgggca gaataacggc tgataacaat atcaaatgcg
    11401 ttatcggcaa atggcagact tgcggcatat ccctggcggg tggtgatatt tttcagttgc
    11461 cgggcttcgg cagcttgtgc cacgacatcc agcatgtggg cagataagtc atacgccacc
    11521 accgcgctca cgttttgcgc agcgccaaac gttggtctgc cctgcggcag acctgacata
    11581 ccttatttat caggcttgcc gggactgaat tcaacgagta gcggattgtg atcggaagcg
    11641 cgcgtaacca gtacagaagc ttcactgacg ttcagaccac ggtagaaaac aaaatcgagc
    11701 gggcgaccaa acgcccgacg gcgctgatca tcggtaaaac gcacctggcg cagcgacatt
    11761 tcccgcgcaa agcgatataa cgcgttcatc cttctacggc tccaggcatt gaaatctccc
    11821 gccataatga ccgggccgct gtggtgagct atctgatcac caataggaag taactgctta
    11881 ctatagacat ccacgcccag actgaagttg acggcgtgta tattaaccac catcaacagg
    11941 cgggtgtcag gtaatggata gaccgtcacc agtgccgact tcgccagacg caaaatgggt
    12001 tctcgttcac gtaacgggca gcaatacact ggatgtgccg ccgaaagggt cattacgcca
    12061 gaaggatgtt gtggcagcac gaaagcgggt acctgatcgg cggcaagata gttagcggtc
    12121 gcaaactgta ccaactctgg cgttgtttgc gcttcctgca ataacaccag atgtgcgtct
    12181 ttgccgtaat tctttaatac cgacaaccat tcagcgcgtt gctgtttgta tatgttccac
    12241 accaggatcc gaatacgctc ttcggtactt aacggttctc cgggtggtaa tgcctggccg
    12301 atgctcgcaa aagaccccgg cggtaagatc ctctccgcag gttgtccggc aacatagcgc
    12361 atggcatagg tgttttttcg cacttttgac tttgaaacct ctattacgtc aacccttccg
    12421 cactcgggag ggttctactg ttatagggat tttagctcac tctttcaacg agcaattgta
    12481 tattgttatg taagcaagtg cttacaccgg tcagaaattt gctcattatt gagaacatta
    12541 aatcggatga catatcacgc atcctgatgg tttaatgaca aataaattca aaatatctca
    12601 tctattttgc aacaggagca aaaatgaaag ccacgtcgga agaactcgcc atttttgttt
    12661 cggtcgtcga aagcggcagc tttagccggg cagcggaaca attagggcaa gcaaactcag
    12721 cggtaagccg ggcggtgaaa aagctggaaa tgaaacttgg cgttagcctg cttaatcgga
    12781 ccacgcggca acttagcctg acggaagaag gcgagcgtta ttttcgtcgc gtacagtcaa
    12841 ttttgcagga gatggcagcg gcagaatcag aaattatgga gacgcgtaat acaccgcgtg
    12901 gactgttacg gatcgatgcc gcaactccag tggtgctgca ctttctgatg ccgttaatta
    12961 agcctttccg tgaacgctat ccggaagtca ctttatcgct agtctcctcc gaaacgatta
    13021 ttaatttgat cgaaagaaaa gtggatgtcg cgatacgcgc tggtacgtta acggactcca
    13081 gcttacgtgc caggccgtta tttaacagtt atcgaaaaat tatcgcctcc cccgattata
    13141 tttcccgcta cgggaagcca gaaacgatcg acgatttaaa gcaacatgtt tgcctgggat
    13201 tcactgaacc cgcttccctc aatacctggc cgatagcctg tagtgatgga caattacatg
    13261 aggtgaagta cggtttgtca tccaatagtg gggaaacact gaaacagctt tgcctgagtg
    13321 gcaacgggat tgcgtgtttg tccgactaca tgatcgacaa agaaatcgct cgcggagaat
    13381 tggtggagtt aatggcagat aaagtgttgc cagtggaaat gccattcagt gccgtctatt
    13441 acagcgaccg tgcggtaagt acgcgcatcc gggcttttat cgatttcctt agcgagcatg
    13501 taaaaacagc tcccggagga gctgtcagag aggcttaatc ccattcagga gccagacctt
    13561 ccgggctaac caggcggtcg ttgcaatcca gtgcggcgat cgctttttta tcttcggcat
    13621 caagctgtaa attttgtgcc ttaagattac tttccaggtt tttacgttta gtagaagaag
    13681 gaattactga gtaaccttcc cccatagccc acgccagaat cacttgtgcc ggagtcgcat
    13741 tgtgtttagc tgcaatacga gcaataacct catctttcag tgccttacca taagccagcg
    13801 tcatatagga agtgatatga ataccgtgct gtttagccca ggcaaccact ttacggtttt
    13861 gcagataagg agagagttca atctggttgg tagcgatgtt ttcagcgcca acagcagcaa
    13921 tcgctttttc catcaacggg atcgtgaagt tggaaatacc gatctcacgt gtcagacctt
    13981 gttttttggc ctccagcagc gcctgcataa actcttcaac agagacttca tcgtttggtg
    14041 acggccagtg gattagcgtc agatcaacat aatcggtacg caatttttgc aggctctctt
    14101 ttagactcgg gatcaatttg tctttgctga gattttcaat ccagatttta gtggtgatgt
    14161 agagttcatg acgtggcacg ccactttctg caatcgcctg acctactgcg gcttcgttat
    14221 catagatttg tgcagtatca attgcgcgat aaccaagttc aagcgccgtt ttcacagatg
    14281 aaataacaac gtcgtctttg agacggaaag tacctaaacc aaatgcaggg atagccataa
    14341 tatgcctctt ttaatgctat tttcgttaac tgaggtgatt atgggcttca gactgttgcg
    14401 gaaaaagagc ataaaatgca gaggattttt gcgattctgg caataataga tatacagacg
    14461 aaaaaaaacc tcgagcgtta actcgaggct tcttaataag tggcggaacg gacgggactc
    14521 gaacccgcga ccccctgcgt gacaggcagg tattctaacc gactgaacta ccgctccacc
    14581 gaattctttt acaaccaccg gttttatgac cggcttactg cttaatttga tgcctggcag
    14641 tttatgaatc acttcgggct gttgcttcgc aacgttcaaa agctttcgct tttgtcctac
    14701 tctcttatcg agtagtgaac tgcctcaaag gtcagtgcca ttcttc
//
LOCUS       DAETQN010000108        14306 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.108, whole genome shotgun sequence.
ACCESSION   DAETQN010000108 DAETQN010000000
VERSION     DAETQN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.108"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            19..1245
                     /gene="rtcB"
                     /locus_tag="K0W68_003260"
     CDS             19..1245
                     /gene="rtcB"
                     /locus_tag="K0W68_003260"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBI3074738.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1249..2265
                     /gene="rtcA"
                     /locus_tag="K0W68_003261"
     CDS             1249..2265
                     /gene="rtcA"
                     /locus_tag="K0W68_003261"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBI3074739.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2308..5013)
                     /gene="malT"
                     /locus_tag="K0W68_003262"
     CDS             complement(2308..5013)
                     /gene="malT"
                     /locus_tag="K0W68_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HBI3074740.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5624..8017
                     /gene="malP"
                     /locus_tag="K0W68_003263"
     CDS             5624..8017
                     /gene="malP"
                     /locus_tag="K0W68_003263"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBI3074741.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8027..10111
                     /gene="malQ"
                     /locus_tag="K0W68_003264"
     CDS             8027..10111
                     /gene="malQ"
                     /locus_tag="K0W68_003264"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBI3074742.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="K0W68_003265"
     CDS             complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="K0W68_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBI3074743.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="K0W68_003266"
     CDS             complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="K0W68_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBI3074744.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="K0W68_003267"
     CDS             complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="K0W68_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBI3074745.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13188..13958
                     /gene="bioH"
                     /locus_tag="K0W68_003268"
     CDS             13188..13958
                     /gene="bioH"
                     /locus_tag="K0W68_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBI3074746.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13987..>14306)
                     /locus_tag="K0W68_003269"
     CDS             complement(13987..>14306)
                     /locus_tag="K0W68_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBI3074747.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3442 a         3568 c         3996 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgctggga acgggtaacc actttattga aatctgcctt
      541 gatgagtcgg agcaggtgtg gattatgctg cactccggtt cacgcggaat tggtaacgcc
      601 atcgggactt actttatcga tctggcgcaa aaagagatgc aggaccaact tgagacgttg
      661 ccgtcgcgtg atctggcgta ctttatggaa ggtacggaat actttgatga ttacctaaga
      721 gccgtggcct gggcgcagct tttcgccagc cttaaccgcg atgcgatgat ggaaaacgtg
      781 gtaacggcat tgcagagcat tacgcagaaa acggtcagac agccacaaac gctggcgatg
      841 gaagagatca actgtcacca caactatgtg caaaaagaac agcactttgg tgaagagatc
      901 tacgtgacgc gtaaaggcgc ggtgtttgcg cgtgctggtc aatatggaat tattccaggg
      961 tcgatgggag caaaaagctt tatcgtccgt gggctgggaa atgaagaatc tttctgttcg
     1021 tgcagccacg gtgccgggcg ggtaatgagc cgtactaaag cgaaaaaact gttcagcgtg
     1081 gaagatcaaa ttcgtgccac cgcgcatgtg gaatgccgta aagatgccga agtgatcgac
     1141 gaaatcccga tggcgtataa agatattgat gcggtgatgg cggcacaaag cgatctggtg
     1201 gaagttatct ataccctgcg tcaggtggtg tgcgtaaaag gataaatgat gaaaaggatg
     1261 attgcgctgg atggcgcaca gggcgaaggt ggcgggcaga tcctgcgctc ggcgctgagc
     1321 ctgtcgatga taaccggcca gccatttacc atcaccagca ttcgtgccgg gcgggcgaaa
     1381 ccggggctgt tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca
     1441 acggtggaag gcgcggagct ggggtcgcag cgtctggtct tccggcttgg caccgtgcgc
     1501 ggcggcgagt accgctttgc tatcggtagt gccggaagct gtacgctggt gctgcaaacg
     1561 gtgctgcccg cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc
     1621 gataacccgt cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg
     1681 aaaataggaa ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc
     1741 ggcgtggtgg caacggaagt ctcgcccgtg gcatcgttta acaccttgca acttggcgag
     1801 cgcgggaaca ttgtgcagat gcgtggagaa gttctattag ctggtgtgcc gcgccatgtt
     1861 gctgagcgtg aaatcgctac actggcgggg agtttttccc tgcatgaaca gaatattcat
     1921 aacctgccgc gcgaccaggg gccgggtaat accgtctcgc tggaagtcga aagtgaaaat
     1981 atcactgaac gctttttcgt cgtcggtgaa aagcgcgtca gtgccgaggt ggtagccgcg
     2041 cagttagtga aagaggtgaa acgctacctg gcaagcccgg cggtagtggg ggaatatctc
     2101 gccgaccagc tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat
     2161 ccttcatgcc atctgctgac caatatcgcc gtggtggagc gtttcttgcc ggtgcggttt
     2221 ggtttgatag aaacagatgg cgtaacgcgg gtgagtattg aatgatgtaa agatgtaaac
     2281 cggatccaat gcattatccg gctaaactca caccccatat cccatcatct tcagcagttt
     2341 ctgcgcgtgt tgtaccgcgg cctgacggtg ggcgacgccg agtttttggt acagattgcg
     2401 gatatgcgtc ttgatggtgg ttgccgccac ttccagctcg cccgcaattt gctcattgct
     2461 gtaaccggag tagatcagcc ccaggacctg ccattcacgt tgggtcaacg ggctggtgtg
     2521 gatcagttct ggcacttccg gatgattcag cagacgttca acgaaattct catcgaaatg
     2581 ggcgaattta tgccgatgat gttgattgat ttctcgcaga atacgctgcg cgcgatgctg
     2641 ttccagttcc ggcagcgtat tgagttgaat cagctggcgc agttgttgcg ccatcgcttc
     2701 gccttcgatg acaaaatgac tgataaatcc ggtgcgattc gccagtttta atgcgtccag
     2761 caacacgcgc tgggcgtcac ttttacgtcc ggcctgccag tacagttgat taagcagcaa
     2821 caggttacgg ttgagatcgc tcatcaaccg cagactgcgg gcattttcat tgagttcttc
     2881 gagaacaatt tccgcaggtt caaactcgcc cagcaagatt tgtgcgcggg caatgttgcg
     2941 ccattgacct tgcaggaagt ggttgttcgc aaactccggt ttagccgtat gacgcaacca
     3001 gttggcagcg gcggctttat cgccggtcat ttgccagtaa atcacccgga ctttgttggc
     3061 gttagagatc cagtcgctgt gatatttgcc attccccagc aggttttcca gacggttcag
     3121 ctggctacgg gcgttatcta aatcaccacg ggccagcgag cattgaatca acattgccag
     3181 gcactgaagc tgttgctgtg gctgataaga cgacaagact tcaatcccgc tacgcgccga
     3241 cgcttcggct tcatccagcc gtgcccaggc ccataacagt tgtgctcgga tgcgcaccag
     3301 aaactcatgc atcggcagct gttccagatg ctgctcgttg atcagctgga acgttttttc
     3361 ctgtgtttcc cacgcggttt gcaggaaccc ttgggcaaac agaatttcac tttgctggat
     3421 taaactccat aaggcgtagt gccagacgtc gtgctggcgt gccatctgtt cggtttgctg
     3481 cattagcgcc agtgagcggg tcaattcgcc tttgcagtgc agcacttcac ccagcaccga
     3541 ggttgctaca atgcggctat agaaccagcc cggtggcagc tcttccagtg ccagttttgc
     3601 cagccgttcc gcttcatccg gattaccatc gttaatcgcc acctgggcgc gcagagcgtt
     3661 aaattctgcg tgcatggtgc cttctctgat gtccttgatt tcatgttcag cacgggctag
     3721 cagggtgtta acttcgccat agcgatgttg gctttgcatt agccacgcct gcaataacac
     3781 taactgcgga ttttccagca aactgtccca cggcagggcc ttaagcgact cttccagcag
     3841 cgacagttcg ctatggttga acaaactcca ggcgtgatta agcagaatat cgcgcagcat
     3901 cagcgcatcg cctgccgcca gcgcatgatg aatcgcttcg ctgggaaatc cctgggccat
     3961 ccagctttct gcggcggcac ggtggatttc cggcagctcc gccgccagtt cccactggca
     4021 gcgctggcgc aggaagttgc caaacagcgg gtgatagcag aaccactcgc cggtatcatc
     4081 catccgctgt aaaaacagcc cctgacgctc aatctcttcg agacgcattt gcccgttttc
     4141 ttcgccagtc acacgggtga tgagggcatc gttcattgag cgcaaaatgg cgcttttcaa
     4201 cagaaaatgg cgcgttgcga gatcgacgtt atccaaaacc tcatcgacca gataatccga
     4261 aagatggctg gcattgattc ccgccaggcg gcgtgccgac ttatgggctg agtgggtatt
     4321 ctgccgggcg gagagggcga ttagctgtag tgccgtcgcc caaccggaaa cgtcatcgca
     4381 aatccaactg ctttctgcgg cttcaatcgg cgatgacaga cggcaatcaa aaaactgctt
     4441 cgcttcctga tgggtaaatg ccagttgctg actgccaatt tccagcagtt ggtcacgaac
     4501 acgcagattg gcaatgccca gttgcggaag gttgcgtgac aacaccacca gggtgagatt
     4561 ttctggttga tggcgaataa agaagcgcat tgactcgtgg atcactggat tagtgatcag
     4621 atgatagtca tcgatgacca gataaagtgg gctatgccat tccgccagct caatgaaaag
     4681 ctgggcgaag agtgacgtca ggctggcata ttgccgtttt tgcgccatcg tctcacatat
     4741 cgcacagtga ccgttggttg cctgctgcac ggcggcaatg agatagctgg cgaaacgctc
     4801 ttgctggtta tcaccttcat ccagcgagta ccagccgata tcgtttttgc ctgccgccca
     4861 ctgggaaatg agggtggtct ttccgtagcc cgcaggactc gtgatcagcg ccagccggaa
     4921 gttgttcgcg ccggaaagtt tagccagcag gcgctcacga accacggtat ggtcgagtcg
     4981 aaccggacga cttagttttg acggaatcag catagttaat cacttcactg tggaaaatga
     5041 ggaaatatta tttttttgcg cttcgtaatt aatggttata aggtcggcca gaaacctttc
     5101 taatgcaagc gatgacgttt ttttatgtgt ctgaatttgc actgtgtcac aattccaaat
     5161 ctttattaac aactcaccta aaacgacgct gatccagcgt gaatactggt ttcccttatg
     5221 ttcatcagat tcatttaagc aagggtttct tcttcattcc tgatgaaagt gccatctaaa
     5281 aagatgatct taataaatct attaagaatg agatggagca cattggatat tttacttgtg
     5341 aaactgtttc actcctttac ttaatttata gagttacctt ccgctttttg aaaatacgca
     5401 acggccattt tttgcactta gatacagatt ttctgcgctg tattgcattg atttgctgct
     5461 aatcctgtgg tttgcactag ctttaagtgg ttgagatcac atttccttgc tcatccccgc
     5521 aactcctccc tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact
     5581 agcgataacg ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt
     5641 taacgataag caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc
     5701 tgcggctgaa atgactcctc gccagtggtg gctagcagtg agtgaagcac tggccgaaat
     5761 gctgcgtgct cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc
     5821 aatggagttt ttgattggtc gcctgacggg caacaacctg ttgaatctcg gctggtatca
     5881 ggatgtacag gattcgttga aggcttatga catcaatctg acggacctgc tggaagaaga
     5941 gatcgacccg gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgactc
     6001 aatggcaact gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt
     6061 ccgccagtct tttgtcgatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa
     6121 ctacccgtgg ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt
     6181 gacgaaagac ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc
     6241 cgttgtcggc tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc
     6301 gcatccgttt gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg
     6361 catcaatgcg gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa
     6421 aaagctgcgc ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg
     6481 tcgccatcat ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct
     6541 gaacgatacc cacccaacta tcgcgattcc agaactgctg cgcgtgctga tcgatgagca
     6601 ccagatgagc tgggatgacg cttgggccat taccagcaaa actttcgctt acaccaacca
     6661 taccctgatg ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc
     6721 gcgccatatg cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac
     6781 ctggccgggc gatgaaaaag tgtgggccaa actggcggtg gtgcacgata aacaagtgca
     6841 tatggcgaac ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc
     6901 ggatctggtg gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca
     6961 taacgtcacc aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc ctgcactggc
     7021 ggctctgttg gataaatcac tgcaaaaaga gtgggctaac gatctcgatc agctgatcaa
     7081 tctggaaaaa ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc
     7141 gaataaagtc cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca
     7201 ggcgattttc gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct
     7261 gctgcatatt ctggcgttgt acaaagaaat tcgtgaaaac ccgcaggctg atcgcgtacc
     7321 gcgcgtcttc ctcttcggcg cgaaagcggc accgggctac tacctggcga agaatattat
     7381 ctttgcgatc aacaaagtgg ctgacgtgat caacaacgat ccactggttg gcgataagtt
     7441 gaaggtggtg ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc
     7501 ggatatctcc gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa
     7561 actggcgctc aatggtgcgc ttactgtcgg tacgctggat ggtgcgaacg ttgaaatcgc
     7621 cgagaaagtc ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc
     7681 aattctggcc aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc
     7741 agtattgaaa gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca
     7801 gatgctgcac agtatcggca aacaaggcgg cgatccgtat ctggtgatgg cggatttcgc
     7861 agcctatgtt gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac
     7921 tcgcgcggcg atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg
     7981 cgattatcag gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg
     8041 tctggataat gccgcgctgg cggcggggat tagccccaat tacatcaatg cccacggtaa
     8101 accgcagtcg attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac
     8161 cgccacgaaa gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa
     8221 aatgccgatg gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg
     8281 aacacagtac aaaggccatg taacgggggg caaagcgttc aatctaccga cgaagctgcc
     8341 ggaaggttat cacacgctga cactcaccca ggacgaccag cgcgcgcatt gccgggtgat
     8401 tgtcgccccg aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg
     8461 tgcctgcgtt cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg
     8521 cgatctcaaa gcgatgctgg tggatgtggc aaaacgtggc gggtcgttca tcggcctgaa
     8581 cccgattcat gcgctctatc cggcaaatcc ggagagtgcc agcccataca gcccgtcttc
     8641 tcgccgttgg ctgaatgtga tttatatcga cgttaaagcc gtcgaagatt tccatcttag
     8701 cgaagaggct caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaagcgcg
     8761 ggatgccgac tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat
     8821 ggcgtggaaa ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt
     8881 tgcagatcaa ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca
     8941 agtgaaagag gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt
     9001 ggattcacca gaagtgcgtc agttctgcga agaacatcgt aatgacgtcg atttttatct
     9061 ctggttgcag tggctggctt acagccagtt tgccgcctgc tgggagataa gccagggcta
     9121 tgaaatgccg attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga
     9181 aacctggtgt gaccgtgaac tatattgcct gaaagcgtcg gttggcgcgc cgccggatat
     9241 cctcggcccg ttggggcaga actggggatt accgccaatg gacccgcata tcatcaccgc
     9301 gcgtgcctat gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt
     9361 acgaattgac catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc
     9421 agatcagggc gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga
     9481 aagtaaacgt catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat
     9541 tgtcggtaag ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga
     9601 ccacgagaag acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc
     9661 gacacatgac ctgcctacgc tacgcggtta ctgggagagc ggggatctaa cgctgggcaa
     9721 aaccctgggg ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact
     9781 ggcgaagcaa gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg
     9841 gcataaggca tcgttgatgt cgatgacgcc gacgctgaac cgtggtttgc agcgctacat
     9901 tgccgacagt aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga
     9961 accggtgaat attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc
    10021 aacgcttgag tcgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag
    10081 acggcgcaga gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc
    10141 cggcagtcag tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac
    10201 cgagataatg gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa
    10261 gtactctttg aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga
    10321 acccaccgca ataaccatca gctctgggct aacacccgtc gttgcaatca gcggtgccgc
    10381 gataccaccc gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc
    10441 agcaatcgac caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat
    10501 gtatttgtcc acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat
    10561 caacagcatc atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat
    10621 tgaacgacca cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgttgc
    10681 cattaccggg tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat
    10741 catttcggca atcgcacgca tcgccatcag cacaaccggc accagagaag tccagacgct
    10801 gacgccaaag ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg
    10861 gattggctta tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt
    10921 cgggattgcc agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt
    10981 ggcaatcgca gtcgggcccg gatgcggtgg caggaagccg tgggtcacag acagtgcagc
    11041 cgccattggt acaccaacat acagcagtgg aatattcgcg gaagccgcga tggtaaacac
    11101 cagcggcagc atcagcacga agcccacttc atagaacagg gcaaaaccaa cggtaaaacc
    11161 ggttaacacc accgcccact ggatgcgctt tttaccaaat ttggcaatca gcgtggtggc
    11221 gatacgttgt gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat
    11281 gatcagggca aggctaccga gcgtcccgcc gacaccgact ttgatggagt caataacttt
    11341 atccagcggc attccttgca ttaatccaac agcaagcgcc acgaggacga gagcgatgaa
    11401 gccgttcatt ttgaagcgga tcatcaggag caacaacaag attacaccga tagcaacaat
    11461 gactaatggc atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg
    11521 acaaactcta attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac
    11581 taaaactaat tattgtagtc agatgtcagg agtatgtttg gtacccatgt gaatgatacg
    11641 ggtaacattt ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg
    11701 tcatattatc gcggggaaac gaagggaggg atttgccaaa gcaatgctgt gccaacgtct
    11761 ggcacatgtt caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa
    11821 agaggggata acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac
    11881 acctttcagc tccgggaatt cgttcagcag ctgcttctcg atcccttctt tcagcgtcac
    11941 atcgaccatg gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc
    12001 ggtgatttcc atcagcgaaa cgcgaccacc gtgaccagca agctgtgggt tgatctgcga
    12061 ctgcagcata tactccacgc gctccatcag cggtgcatcg tctgccactt tacgcatttt
    12121 ggcgttcggg gctttcagcg ttaactgaga acccaactgg tcggtaacaa agtcgatctc
    12181 tgcatcttcc aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt
    12241 cagggcagtg tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc
    12301 gttaggcgtg ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc
    12361 cagcagtttg gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta
    12421 atagttgact attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac
    12481 aaaggcacca gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg
    12541 cgacggtact tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacgg
    12601 gcaattcaag acgaaaggca tttttcaggt tgcgcttgcg cagccgggca ctgagaaaat
    12661 gctgggtcgc agtggcccgt gtacgtgtga cggcttcgct atcccattgg cagtgcaacc
    12721 agcgtgataa cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc
    12781 gctgccataa cggaacgctg atgatgcgat ccggcaattg caacccggtg gtgcgacgag
    12841 cgtgtaagac ttccaatagt aacagacgtg acagggcgct ggcgatttca ctgcgccggg
    12901 aaaatttaag ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca
    12961 gtctttgcca gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg
    13021 cgggtaatcc acattgtggg cataacgttt tatctgtgcg ggtggcgcgt gaacagaccg
    13081 aacaaatccc ccaatgacct aacgccagtg gcattcggca tagccagcat aatcccggta
    13141 ctgttagcat atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacattt
    13201 ggtggcagac caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga
    13261 atgccgaagt gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcaccttg
    13321 ttgatctgcc cggcttcggg cgcagccagg gatttggtgc gctgtcactt gctgatatgg
    13381 ccgaagccgt gctgcaacag gcacctgata aagccatttg gttaggctgg agtctgggcg
    13441 ggctggtggc aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg
    13501 tggcgtcgtc accttgtttt agtgctcgtg acgattggcc ggggataaaa ccggacgtgc
    13561 tggcgggatt tcagcagcaa ctcagtgatg attttcagcg tacagtggag cggttcctgg
    13621 cgttacaaac catggggact gaaacggcgc gccaggatgc gcgggcgttg aagaaaaccg
    13681 ttctggcgtt accgatgccg gaggttgacg tgcttaatgg cgggctggaa atcctgaaaa
    13741 cggttgatct ccgtcagccg ctgcaaaacg tgcccatgcc gtttttgcga ttgtatggct
    13801 atctcgatgg tctggtgccg cgcaaagtgg tgccgatgct ggataaactc tggcctcgca
    13861 gcaaatcata tatcttcgcc aaagcggccc atgcgccgtt tatttcgcat ccggtcgagt
    13921 tttgtcacct gctggtggcg ttgaagcaga gggtgtaggc aacttttgaa atggcgagac
    13981 agaaatttac tggctcgccg cagccaactc ttcttctgac accccggtaa agcgcatgat
    14041 gtccgcaaga gggactccgg attcaagcat tattttggct atatgcaggg ctttggattg
    14101 ttccccctcc tgccgcaatc tttccgcaat tgtcattaaa ctctccttgt gtttcggtga
    14161 acgttcggca acgccgtcga taaaatcgtt aaaacgtacc gcgtcgccgg tttgcagtat
    14221 gtaattaaac agccctttga tttgtctgtc attagcgtat ccactactta ataagcaggc
    14281 catttgctct accagcccca tcaggt
//
LOCUS       DAETQN010000109        14159 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.109, whole genome shotgun sequence.
ACCESSION   DAETQN010000109 DAETQN010000000
VERSION     DAETQN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14159
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.109"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..228)
                     /locus_tag="K0W68_003270"
     CDS             complement(<1..228)
                     /locus_tag="K0W68_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HBI3074748.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            385..561
                     /locus_tag="K0W68_003271"
     CDS             385..561
                     /locus_tag="K0W68_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HBI3074749.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
     gene            1187..1486
                     /locus_tag="K0W68_003272"
     CDS             1187..1486
                     /locus_tag="K0W68_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074750.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     ILNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="K0W68_003273"
     CDS             complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="K0W68_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBI3074751.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(2472..2939)
                     /locus_tag="K0W68_003274"
     CDS             complement(2472..2939)
                     /locus_tag="K0W68_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBI3074752.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3104..3973
                     /gene="pldA"
                     /locus_tag="K0W68_003275"
     CDS             3104..3973
                     /gene="pldA"
                     /locus_tag="K0W68_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBI3074753.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4106..5935
                     /gene="recQ"
                     /locus_tag="K0W68_003276"
     CDS             4106..5935
                     /gene="recQ"
                     /locus_tag="K0W68_003276"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBI3074754.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5999..6616
                     /gene="rhtC"
                     /locus_tag="K0W68_003277"
     CDS             5999..6616
                     /gene="rhtC"
                     /locus_tag="K0W68_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBI3074755.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="K0W68_003278"
     CDS             complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="K0W68_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBI3074756.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7409..8431
                     /gene="pldB"
                     /locus_tag="K0W68_003279"
     CDS             7409..8431
                     /gene="pldB"
                     /locus_tag="K0W68_003279"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBI3074757.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8439..9239
                     /gene="yigL"
                     /locus_tag="K0W68_003280"
     CDS             8439..9239
                     /gene="yigL"
                     /locus_tag="K0W68_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBI3074758.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9315..10214
                     /gene="bioP"
                     /locus_tag="K0W68_003281"
     CDS             9315..10214
                     /gene="bioP"
                     /locus_tag="K0W68_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBI3074759.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(10102..11055)
                     /gene="metR"
                     /locus_tag="K0W68_003282"
     CDS             complement(10102..11055)
                     /gene="metR"
                     /locus_tag="K0W68_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBI3074760.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11291..13552
                     /gene="metE"
                     /locus_tag="K0W68_003283"
     CDS             11291..13552
                     /gene="metE"
                     /locus_tag="K0W68_003283"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBI3074761.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13837..14082)
                     /locus_tag="K0W68_003284"
     CDS             complement(13837..14082)
                     /locus_tag="K0W68_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBI3074762.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3446 a         3424 c         3690 g         3599 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac atttttacat tgttttatat atataattgt tttgaatata tattacctga
     1021 attatgtggt ttgcatatat atatgcaata tagcagtctt tgcattgata actatacagt
     1081 agtaatttag agtgtgtaag tttttattga tggggtatat tttaattaaa tataattgaa
     1141 atgtattttt attctataaa caatatcttg ggtgaatgga ttctatatgg gaaatggtaa
     1201 cttttggcag ttgttcttgg gtaaagaacc ttacagcaaa aaatctgatt ttgttaaggc
     1261 atggttattg tggtgggcta ttaccatcct tgttctattt attttaagcc tgactacgat
     1321 attaaattta agaagtatag gtatagggtt aagttttggg ttttgtcaaa tccttggtag
     1381 atatacgatg tctctaggat ggagtaagta ctggggagtt ttgggggtat ttcctgtaac
     1441 aaatattgtt ctttttctta tcttaatttt tttcaaaaaa agataaataa gtcagtgggt
     1501 aggctcttta aacggcctac ccatgttaat tcttaacttt ttctacgctg cgtataaatc
     1561 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg
     1621 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
     1681 tactggaaaa agcctaacgt tgagagacgc aagcgcgtgg cagcggcggt aaaacacaac
     1741 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
     1801 tgccccatat ggctggttga gctgtcggca atagcaaaca ggtaaattgc cgccacgggc
     1861 agcagccaca tggtttcgat taacatgccg gtttgcgctt caacggcaat cttcttgcgt
     1921 accagaccgt agaaggcaaa actaaatgcc agtcccagcg cgataatagg tagcgaacca
     1981 aaagtccaca gctggactaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
     2041 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
     2101 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
     2161 cagttgccac caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga
     2221 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
     2281 aacgaccaga tcacgcgatg cgtcaggatt tcatcggcgg gcacgtagta aatcaacttg
     2341 aagtacgctg gcgctatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
     2401 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
     2461 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
     2521 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     2581 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     2641 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     2701 gtacttccca cgcacaccag accggcggcg acatccagcg ccgacgcaat gaccccgccg
     2761 tgcaaaatgc tttgcgccca gttgcccacc atcattggct gatttttaaa ggccagctgt
     2821 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     2881 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     2941 tcatccttac acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     3001 ctactttagg aagggataac taacggctat ggagttaagt atgtaaaata gcccgcagaa
     3061 aaatattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     3121 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     3181 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     3241 tgacaatccg ttcacgctct atccttatga caccaactac ctcatttaca cccaaaccag
     3301 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     3361 agtaaagttt cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccgaactc
     3421 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     3481 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     3541 ctgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
     3601 gcgttccgac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     3661 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     3721 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     3781 ggtgttgagt gcgaaaggac agtacaactg gaataccggt tacggcggcg cggagttagg
     3841 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     3901 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtaggag ttatgctaaa
     3961 cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaacccagca tccaggatta
     4021 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     4081 tttatttcag gcaatcgggg ggaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     4141 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
     4201 aattatcgac actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg
     4261 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     4321 accgctgatt tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc
     4381 ggcgtgcctt aactcgacgc aaacccgcga gcagcaactt gaagtgatga caggctgccg
     4441 caccgggcaa attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct
     4501 tgagcatctg gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc
     4561 ccaatggggc cacgatttcc gcccggaata tgccgcgctt ggtcagttac gccagcgttt
     4621 cccgaccctg ccgtttatgg cgctgactgc cacagccgac gacaccacgc gccaggatat
     4681 cgtgcgcttg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     4741 tattcgctac atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca
     4801 ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     4861 cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa
     4921 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc
     4981 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     5041 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     5101 gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct
     5161 ggaagagaag ccgcaggggc agttgcagga tattgagcgc cacaaactca atgcgatggg
     5221 cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg
     5281 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     5341 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat
     5401 gggttatgtg gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca
     5461 tgacaaactg aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt
     5521 gatccgccag ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct
     5581 acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     5641 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     5701 tcgtaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt
     5761 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     5821 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     5881 taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc
     5941 agcataaaaa agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     6001 gttgatgtta tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc
     6061 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     6121 cgtgctgggc attacctgcg gtgtaatggt ttgggctggg attgcgttgc ttggcctgca
     6181 tttgattatc gaaaaaatgg cctggctgca tacgcttatg gttggcggtg gcctgtatct
     6241 ctgctggatg ggttaccaga tgctgcgcgg cgcgctgaaa aaagaggcgg tttctgcacc
     6301 tgcgccacag gtcgagctgg cgaaaagtgg gcgcagtttc ctgaaaggtt tactgaccaa
     6361 tctcgctaat ccgaaagcga ttatctactt tggctcggtg ttctcattgt ttgtcggtga
     6421 taacgttggc actaccgcgc gctggggcat ttttgcgctg atcattgtcg aaacgctggc
     6481 gtggtttatc gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg gttatcaacg
     6541 tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt
     6601 gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc
     6661 gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata
     6721 aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga
     6781 gcaatccgtt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg
     6841 gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga
     6901 aatagcgccg ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc
     6961 tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taaggtcaat tgcaccagcg
     7021 gcgcgccact gctggattcc cagccaaatc aagtaagccg cgcctgccca cttcaacact
     7081 tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata
     7141 tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg
     7201 ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc
     7261 gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat
     7321 ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa aaaaacgatt
     7381 ttacgtggtt taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag
     7441 agaaaacgcg tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga
     7501 tgaagcagag tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca
     7561 gcaccatgac cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc
     7621 ggaactggcc tatgacctgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg
     7681 gcagggacgt tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa
     7741 tgattatgtt gatgatctgg cggcattctg gcagcaggag gttcagcccg gtccgtggcg
     7801 taaacgctat atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg
     7861 ccatccaggt gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg
     7921 tatgccgtca tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg
     7981 tgatggctat gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact
     8041 gacccacagc agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat
     8101 tcgcgtcggt gggccgacct accattgggt acgcgaaagt attctggctg gcgaacaggt
     8161 gttagccggt gcgggtgatg acgccacgcc aacgcttctc ttgcaggctg aagaggaacg
     8221 cgtggtggat aaccgcatgc atgaccgttt ttgtgaactc cgcaccgccg cgggccatcc
     8281 tgtcgaagga ggacgaccgt tggtaattaa aggtgcttac catgagatcc tttttgaaaa
     8341 ggacgcaatg cgctcagtcg cgctccacgc cattgttgat tttttcaaca ggcataactc
     8401 acccagcgga aaccgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg
     8461 atttagatgg cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaaccc
     8521 tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg
     8581 atgtggggca aattcgcgat aatctggaga tcaagtcgta catgatcacc tccaacggcg
     8641 cgcgcgttca cgatctggat ggcaatctga tttttgctca taacctggat cgcgacattg
     8701 ccagcgatct gtttggcgta gtcaacgaca atctggacat cattactaac gtttatcgcg
     8761 acgacgaatg gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg
     8821 tgttcaaata tgcgctgtat gagcctggat tactggagcc ggaaggcgtc agcaaagtgt
     8881 tcttcacctg cgattcccat gaacaactgc tgccgctgga gcaggcgatt aacgctcgtt
     8941 ggggcgatcg cgtcaacgtt agtttctcta ccttaacctg tctggaagtg atggcgggcg
     9001 gcgtttcaaa aggccatgcg ctggaagcgg tggcgaagaa actgggctac agcctgaagg
     9061 attgtattgc gtttggtgac gggatgaacg atgccgaaat gctgtcgatg gcggggaaag
     9121 gctgcattat gggcagtgcg caccagcgtc tgaaagacct tcatcccgag ctggaagtga
     9181 ttggtactaa tgccgacgac gcggtgccgc attatctgcg taaactctat ttatcgtaat
     9241 cgttctttat ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca
     9301 aagagttttc cattgtggcg ctacttatca tcaccacgat tctgtgggcc ttctccttta
     9361 gcttttatgg cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg
     9421 ttggcctggc ggcactcgtt tttctgccgt ttctgcgtac ccgtggcaat agcctgaaaa
     9481 cggtcggcct gtatatgctg gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt
     9541 tccgcgctta tctctacctg acggtctccg aattgttgct gttcaccgta ctgacgccgc
     9601 tctacatcac gctgatttat gacatcatga gtaagcgccg tctgcgctgg ggctatgctt
     9661 ttagcgcctt gctggcggtg attggtgccg ggattattcg ctatgatcag gttaccgacc
     9721 atttctggac tggcttgttg ctggtgcaac tctccaatat cacttttgcc atcggcatgg
     9781 tgggttacaa acgcctgatg gaaactcgcc cgatgccgca gcataacgcc tttgcgtggt
     9841 tctatcttgg cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga
     9901 aaatgccgca aaccacgttg caatggggca ttctggtgtt tcttggcgtg gtggcttccg
     9961 ggattggcta ctttatgtgg aactacggcg cgacgcaggt ggacgccgga acgctgggca
    10021 ttatgaataa tatgcacgtt ccggcagggc tgctggtaaa cctggctatc tggcaccaac
    10081 agccgcactg gccaacgttc attacaggcg cgctggtgat cctggcctca ctgtgggtgc
    10141 atcgtaagtg ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg
    10201 cgctgagcga ataaatgctt ctgtaatcgg ctgacgctgc tcgccatcgc gcacggcggc
    10261 gtacagtcgg ctccacaagc cttcgcccag ggttttggtc accaccagac cttggcgctc
    10321 aaaactctct actacccaat gcggtagcgc ggcaataccc atccgcgcgg caaccatctg
    10381 aatcaacaat aaggtgttat cgacgctttt cagtgacggg ctgacgcctg ccggctgaag
    10441 aaaatgccgc cagacatcca gtcgactacg ctgcaccgga taaatcaaca gcgtctcgct
    10501 ggcgagatct tccggtgtaa ttcgcgtttt cgccgccagt ggatggtcag gtgctaacac
    10561 cagacgcact tcatagtcga acatcggcga ataatgcagg ccactgcgcg gcaggatatc
    10621 ggaggtcatc accagatcca gctcgccctg ttgcaacgca ggctgcgggt caaacgtcac
    10681 gcccgattta aaatccatct ctacctgcgg ccagttctta tggaaatttt ctaacgcggg
    10741 cgtcagccac tgaatacagc tatggcactc aatggcaatg cgcagacgcg tctgctgcgg
    10801 ttcattgcag gcttgcagtg cctggctgat ttgcggcagc acctggtttg ccagttgcaa
    10861 caggatttct ccctgcggtg taaagcgtag cggctggctc ttacgcacaa atagccggaa
    10921 gccaaggcgt tgttccagat cgctaaactg gtgagacagg gcggattgcg tctgatgcaa
    10981 cgtcgccgca gcggctgcga gagagccgca gttccgcaac gcttgtagcg ttttcaggtg
    11041 ttttacttcg atcatgaaag tccttcactt cggcatgaat aatttgcgct tgaggaatat
    11101 acagtaaccg ccaattatgg atgtgtaaac atctggacgg ctaaaatcct tcgtctttta
    11161 aatttatggt gcgttggctg cgtttctcca ccccggtcac ttacttcagt aagctcccgg
    11221 ggatgaataa acttgccgcc ttccctaaat tcaaaatcca taggatttac atataattag
    11281 aggaagaaaa atgacaatat tgaatcacac cctcggtttc cctcgcgttg gcctgcgtcg
    11341 cgagctgaaa aaagcgcaag aaagttattg ggcggggaac tccacgcgtg aagaactgct
    11401 gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga
    11461 cctgctgccg gtgggcgatt ttgcctggta cgatcatgta ctgaccacca gtttgctgct
    11521 gggtaatgtt ccgccacgtc atcagaacaa agacggttcg gtagatatcg acaccctgtt
    11581 ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa
    11641 atggtttaac accaactatc actacatggt gccggagttc gttaaaggcc aacagttcaa
    11701 actgacctgg acgcagctgc tggaggaagt ggacgaggcg ctggcgctgg gccacaacgt
    11761 taagcctgtg ctgctggggc cggttactta cctgtggctg gggaaagtga aaggtgaaca
    11821 gtttgaccgc cttagcctgc tgaacgacat tctgccggtt tatcagcaag tgctggcaga
    11881 actggcgaaa cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg tactggaact
    11941 accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg gacaggtgaa
    12001 actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct
    12061 gcctgttcag ggtctgcatg ttgatctcgt acatggtaaa gatgacgttg ctgaactgca
    12121 caagcgcctg ccttctgact ggttgctgtc tgcgggtctg atcaatggtc gtaacgtctg
    12181 gcgcgccgat cttaccgaga aatatgcgca aattaaggac attgtcggca aacgtgattt
    12241 gtgggtggca tcttcctgct cgttgctgca cagccccatc gacctgagcg tggaaacgcg
    12301 tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc atgaactagc
    12361 actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt ggagcgcccc
    12421 aattcaggcg cgtcgtcact ctactcgtgt acataatccg gcggtagaaa agcgtctggc
    12481 ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg ctgaagccca
    12541 gcgtgcacgt tttaaactgc cagcgtggcc gaccaccacg attggttcct tcccgcaaac
    12601 cacggaaatt cgtaccctgc gtctggattt caaaaagggc aatctcgacg ccaataacta
    12661 ccgcacgggc attgcggaac atatcaggca ggccattgtt gaacaggaac gtttgggact
    12721 ggatgtgctg gtacatggcg aggccgagcg taatgacatg gtggaatact ttggcgagca
    12781 cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt
    12841 gaagccaccg attgttattg gtgacgttag tcgcccggca ccgattaccg tggagtgggc
    12901 gaagtatgcg caatctctga ctgataaacc ggtgaaaggg atgctgactg gcccggtgac
    12961 tattctctgc tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat
    13021 tgcgctggcg ctgcgtgatg aagtggccga tctggaagcc gctggaattg gcatcatcca
    13081 gattgacgaa ccggctctgc gcgaaggttt accgctgcgt cgtagcgact gggatgcgta
    13141 tctccagtgg ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag atgacacaca
    13201 aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcga ttgcggcact
    13261 ggacgcagac gtcatcacca tcgaaacctc gcgttctgac atggagttgc tggagtcgtt
    13321 cgaagagttt gattatccga atgaaatcgg tccaggcgtc tatgacattc actcgccaaa
    13381 cgtaccgagc gtggaatgga ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc
    13441 agagcgcctg tgggttaacc cggactgtgg cctgaaaacg cgcggctggc cagaaacccg
    13501 cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg cgtcggggat gatgaatatt
    13561 aacgagtcac tgtaaattgc agtacgatga aagaggcaaa actgcctgat actaggccta
    13621 cataatttat gcaatttatt gaatttttgg gttttgttgg ccggacaagg cgttcacgcc
    13681 gcatccggca tgaacaacgc gcactttgcc aataatctga tggcttccat caccggaagc
    13741 cattttgcta tctgcgcccg ttcagataca gaaataaccg aatcattttt acgcaaaata
    13801 taatcctgag cgatttgttc agtaatcaac gctacgttat ttattctcac ttttcacgaa
    13861 attgtatccc ttttcagtaa cgtggtagtg aggcatagac ttatgtccgc tatcctcaat
    13921 aataaccaaa ccctcgttag ctaatttgtt aacaatgtta acaacattac cgaatcctgg
    13981 aatttgcctt atggatagtg tgcgatcgag attcatccag ttccagttta tatctttctc
    14041 agccataatt ttcagtacgt taatttcagt ttcagttatc atagtcaagt gcatcctggt
    14101 ggtataagtc attaagttct cccttaagtt tataatcttc tagagtagca gtatgcgta
//
LOCUS       DAETQN010000110        14108 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.110, whole genome shotgun sequence.
ACCESSION   DAETQN010000110 DAETQN010000000
VERSION     DAETQN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14108
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.110"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(14..1372)
                     /gene="hprS"
                     /locus_tag="K0W68_003285"
     CDS             complement(14..1372)
                     /gene="hprS"
                     /locus_tag="K0W68_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HBI3074763.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(1372..2043)
                     /gene="hprR"
                     /locus_tag="K0W68_003286"
     CDS             complement(1372..2043)
                     /gene="hprR"
                     /locus_tag="K0W68_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBI3074764.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2176..2589
                     /gene="hiuH"
                     /locus_tag="K0W68_003287"
     CDS             2176..2589
                     /gene="hiuH"
                     /locus_tag="K0W68_003287"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBI3074765.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2698..3702
                     /gene="msrP"
                     /locus_tag="K0W68_003288"
     CDS             2698..3702
                     /gene="msrP"
                     /locus_tag="K0W68_003288"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HBI3074766.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            3703..4338
                     /gene="msrQ"
                     /locus_tag="K0W68_003289"
     CDS             3703..4338
                     /gene="msrQ"
                     /locus_tag="K0W68_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBI3074767.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            4595..5245
                     /gene="zinT"
                     /locus_tag="K0W68_003290"
     CDS             4595..5245
                     /gene="zinT"
                     /locus_tag="K0W68_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBI3074768.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            5588..6118
                     /locus_tag="K0W68_003291"
                     /pseudo
     CDS             5588..6118
                     /locus_tag="K0W68_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(6688..6777)
                     /locus_tag="K0W68_003292"
     tRNA            complement(6688..6777)
                     /locus_tag="K0W68_003292"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6741..6743),aa:Ser,seq:cga)
     gene            6871..7668
                     /gene="mtfA"
                     /locus_tag="K0W68_003293"
     CDS             6871..7668
                     /gene="mtfA"
                     /locus_tag="K0W68_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBI3074769.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7769..7844
                     /locus_tag="K0W68_003294"
     tRNA            7769..7844
                     /locus_tag="K0W68_003294"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7802..7804,aa:Asn,seq:gtt)
     gene            8158..14108
                     /gene="yeeJ"
                     /locus_tag="K0W68_003295"
                     /pseudo
     CDS             8158..14108
                     /gene="yeeJ"
                     /locus_tag="K0W68_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         3990 a         3354 c         3171 g         3593 t
ORIGIN      
        1 ctgaagccct atgttaattt ctttgcggta acataatccg gaacacatta tgcttattga
       61 gatagtgata cgtagcactt cccccatgta attcggcaat cgctttgact aaagaaagac
      121 caagtccctg acctacggaa tgacgcgaat tatctccccg ccaaaatcta cggaagagtt
      181 tttcaggctc atgaattttc gttccaggac tggcgacatc aatattaaga tagccgttgg
      241 tatcaagaaa actggttata tgaatacgcg atttttctgg tgaatatcta atggcattaa
      301 caataagatt cgataacatt cgttgcagta aaattttatc cgcaaagatt tgctgattgc
      361 actcgacctt aaagcaaatc tctttctcgt ctgaaaggta ttcaagataa tccaacaaat
      421 tttcgacttc cttattgaga gaaagcgagt ccagtttcac caaaacattg tttttatctg
      481 cccgggcaag aaacagtatg ttctctgtta accgcgaaat attttccagc tcttcaatgt
      541 taccggcaat tgttttttga tattcagcga tacttctggt ttgactgagc gtaacctgat
      601 tctgacccag taatgcatta atgggcgttc taagttcatg agcgagatcg tcagcaaatt
      661 gacttagacg ctcaaaatct ttgactaaag catgatgcat tttattcaac gcctgcccaa
      721 gaggttttag ttctctcggt aatgcgctaa cctcaacagg ctcccggcta tcgttataat
      781 tcagcgcttc cgttacacca ctcaactttt tgatctctcg taatcccgtt ctgattaaca
      841 gcggacttaa tactgagcaa agtacaatgg cgacaatgca aattataatg ctattaattt
      901 tatactgttc aagcatgtta tgtctggctg aagccaattt agccacagta accgttaatg
      961 atggcgaaac ttcatcaata ttaattcgta aagcatcaat ctcagtatca ttaataatgc
     1021 ttctgtaaat gccagctgcg ctgattgtct cactagcagg tatgttattt aacatgtcat
     1081 cactgacatt tgtccggtta acaattttat tgatgccatc accatgaatg atcaagatat
     1141 cctgactaac atccatcatc cggttaaagt atacaggtaa cgtatctgga tttaccccat
     1201 caattaacaa ctgcttgatc tgtgctgtcc ggttaatgag tgttgtatca tcgcgccatt
     1261 tcaactcact tgccaggcca ttatagagag tccagacaat tccagcgcca gcaacagaca
     1321 gcagcaatat aaaaagcaag gttaaacgga cggttataga taatcttttc attatttttt
     1381 taccgctacg aatgaatagc ccatcccccg gattgtggcg attagctttt caggaaaagg
     1441 atcatcaact tttgcgcgga gcctgcgaat ggcgacatcc accgtattgg tatcactatc
     1501 aaagttgatt ccccaaattt cactcgcaat aaccgttctg ggtataattt cgccagctct
     1561 ggaggccagt agccaaagta actgaaactc cttgcgcgtc agtgtaatac tgatattgtt
     1621 cctgctcaca ctttgactta cagagtccat tcttaagcca ctgatttcta atgttgaatt
     1681 caaagcgtga tgttgcctta attgtgcccg aacccttgcc agcaactcag aaaatgaaaa
     1741 aggttttacc agataatcat ttgctccact gtccagccct ctgactctgt catcgacaga
     1801 atcccttgca gtaaggcaaa taacaggggt ttgctttgct gttcttaacg tttgtaagat
     1861 ctgccagcca tccatacccg gaagcataat atccagaatg atcaatgcat aatcatcctt
     1921 cagcgcaaga taaagcccat ctctgccatc agaaacggca tcaatgacat aacccgcttc
     1981 ggaaagcccc tgcgttaccc actcctgggt cctttgatta tcttcaataa gtaaaatctt
     2041 catgaaaata atatgccata aatcatatgt tatagaaaca gcctagttca ttacaaaatt
     2101 gtaatgctgc tgtaaggtta ccctggccgc tttttcgcta tcctcaaaac tcattcacat
     2161 gacaaggata taaacatgtt aaagcgttat ttagtactct ccgtagtaac ggcagcattt
     2221 tcattacctt ctttggttta tgccgcacaa caaaacattc ttagcgtgca cattttgaac
     2281 cagcaaaccg gaaaacccgc tgccgacgtg acagtcactc ttgaaaagaa agcggacaac
     2341 ggctggttac aacttaatac cgccaaaaca gataaggatg gacgaattaa ggcactgtgg
     2401 cccgagcaaa ctgcaactac gggcgattac cgtgtcgtat ttaaaaccgg ggactatttc
     2461 aagaaacaaa atcttgaaag tttcttccct gagatccccg ttgaatttca tattaataaa
     2521 gtgaacgagc attatcatgt gcctttatta cttagccaat atgggtattc aacctatcgt
     2581 ggcagttaat gcataaatat gtaaaagcag atctctgcta ctgttaggga ggacactccc
     2641 tgacagatta acagtaaacg gctcttgctg gctaacgaca aaaaagtgtg atggcttatg
     2701 aaaaagaatc aatttttaaa agaatcagat gttacggccg agtcggtatt ctttatgaag
     2761 cgtcgacagg tgttaaaagc actgggcatc agcgcagctg cactttcttt gcctcacgct
     2821 gcgcatgccg atctgcttag ctggtttaaa gggaacgatc gcccgcccgc ccccgccgga
     2881 aaaccgctgg agttcagcaa gcctaccgcc tggcaaaata atctgccact gacgccagta
     2941 gataaagtct ccggttataa caacttctat gaattcgggc tggataaagc cgatcccgcc
     3001 gctaatgctg gtagcctgaa aaccgatcca tggacactga aaatcagcgg cgaagtggca
     3061 aaaccattga ccctcgatca cgatgattta acccgtcgct tcccgctgga agagcgtatt
     3121 tatcgtatgc gctgcgtgga ggcatggtcg atggtggtgc cgtggattgg ttttccgctg
     3181 cacaaattgc tggcgcttgc cgaacccacc agcaatgcga agtatgtcgc tttcgaaaca
     3241 atttatgcac cggaacaaat gccaggccag caggaccgct ttatcggcgg cgggctgaaa
     3301 tatccttatg tcgaaggatt gcgtctcgac gaagcaatgc atccgctcac actgatgacc
     3361 gtgggtgttt atggcaaggc gttaccgcca caaaatggcg cgccggtacg actgattgtg
     3421 ccgtggaaat atggctttaa agggattaaa tcgatagtca gtattaagct gacccgcgag
     3481 cgtccgccaa ccacctggaa tctggcagcg cctgacgaat acggttttta cgccaacgtt
     3541 aatccgcatg ttgatcaccc gcgctggtca caggctaccg aacgatttat tggttcaggc
     3601 ggcatcctcg atgttcagcg ccagccaacg ctactgttta atggttacgc cgaccaggtg
     3661 gcatcgctgt atcgtggcct ggatttgcgg gagaatttct gaatgcgtct gacagcaaaa
     3721 caggtgacat ggctgaaagt tagcctgcat cttgccggat tgttgccgtt tctctggctg
     3781 gtctgggcga tcaatcacgg tggactgggt gccgatccgg tgaaagatat tcagcatttt
     3841 accggtcgca ctgcactgaa atttttgctg gcgaccttgt taatcacacc tctggcacgc
     3901 tacgcaaaac agccgttatt gatacgcact cgccgcctgt taggcttatg gtgctttgcc
     3961 tgggcgacac tgcatttaac cagttacgca ttgctggagc tgggcgtgaa taatctggcg
     4021 ttattaggca aggagttaat tacccggcct tatttaacgt taggaattat cagctgggtt
     4081 attttgcttg ctttagcgtt tacttcaacc caggcgatgc agcgaaaact cggcaagcac
     4141 tggcaacagt tgcataattt cgtctatctt gtcgcgatcc tggccccaat acattatctg
     4201 tggtcagtga agattatttc accgcagccg ctcatctacg ctgggctggc tgtactgctt
     4261 ttagccttac ggtataagaa gttgcgttcc ctttttaagc ggttacgcaa gcaggttcac
     4321 aacaaattat ctgtataact tcatattgcc gacaaagtac gcgttatccc tgccggatgt
     4381 ggcgcgagcg ccctgtccgg cctgcgcaga tcacatcagg ttatgtttcg tttgtcatca
     4441 acctcaaatt atcaacataa ttcattttat ttcaataaga tagataagta cacctgagaa
     4501 aactatgctc tcatctccta agagttgttg cattttgcca tatgttacaa tataacatta
     4561 cacatcatat acaatgactc tggaggaaac tgttttggca attcgtcttc acaaactggc
     4621 tgttgcttta ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc atcactcaca
     4681 cggcaaaccc ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg atgatgccaa
     4741 tgtacaaaac cgaacgctca gtgactggga tggagtctgg caatccgttt atcctttact
     4801 gcaaagtggc aaacttgacc ccgtctttca gaagaaagcg gatgcagata aaattaaaac
     4861 atttgctgaa attaaagatt attatcacaa aggttatgca acagatatcg agatgattgg
     4921 cattgaggac ggcattgttg aattccatag aaataatgaa acaacatcct gtaaatatga
     4981 ttacgatgga tacaaaatac tcacctataa atcaggcaag aaaggcgttc gttacttatt
     5041 cgaatgtaaa gatcctgaaa gcaaagcccc taaatatata caatttagcg atcatataat
     5101 tgcaccacga aaatcatctc attttcacat ttttatgggt aatgattcac agcaattttt
     5161 attaaatgaa atggaaaact ggccaacgta ttatccatat cagttgagta gcgaagaagt
     5221 ggtcgaggaa atgatgtctc attgagtatt ctcatgataa cgcctcgatg ccgctttagt
     5281 aagtcatcat aactgccact ggtccatcca caaacgccac tgaacgcaag ctagctacag
     5341 acacgctcat cactatgacg tgtctgtata ttaataagct aacccgcatt gagttaacca
     5401 ataacggatt ccatacacaa tacggccaac agagaaagat acctgtgctc acgccattgc
     5461 ttatattggc tggttacaat gtgcactatc aattttttaa ataacaaatt cttaaaacca
     5521 catctgtata aggaattttt aaggttcgtg ggatagcttg actgtaaaaa ttacaggagc
     5581 tacaaaaatg aaccgattct caaaaactca aatttattta cattggataa cgctgctttt
     5641 cgttgcaata acctatgccg cgatggaact ccgtggctgg tttcctaaag gtagtagtac
     5701 ttatctgctg atgcgagaaa cacattacaa tgcgggtata ttcgtttggg tgttaatgtt
     5761 ttcacgcctg attataaaac accgttatag tgatccttct attgtaccac cgccacctgc
     5821 ctggcaaatg aaagcggctt cgctaatgca catcatgctt tatataacct tccttgcact
     5881 tcctctgctg gggattgctt tgatggctta cagtggaaaa tcgtggagtt tccttggttt
     5941 caatgtgtct ccctttgtta ccccaaacag cgaaattaaa gcactgataa aaaatattca
     6001 cgaaacctag gcaaatatag gttacttttt aatcgcagct cacgctggcg cagcactctt
     6061 tcatcactac attcagaaag ataatactct gttacgaatg atgcatcgcc gcaaataagt
     6121 cgatcccctg acaaggaaat gtgaaccatg tacatcaacg cgtcataaat gtttacgcaa
     6181 gtgcacaatg catcctttct actatcaaca aggaggagca gaaactgtgt aaatcgtaca
     6241 atggtaagac ttatcaaaaa tacaggttaa ttttggtatt taaacctttt ttacttgtta
     6301 gtgtgataca aaggctttca aaaaagctgg gaaattcaac aactcttgga aattatggac
     6361 tttgtcccgc ggctttaatc tgacggccgc gttccttttt tgactcaatt attgacccct
     6421 tctctacgca acttcagttc ccaccaccaa ctttgcggca gtttcatagg atgaatgtct
     6481 aaaagaataa tggtgagcga taagaaaacg actgaataac tgcacatttt cgctcgaaag
     6541 cttcctgtca gatccatagc gaatcaagtg ctgaatgtca cagtatcgaa cagaaaacag
     6601 tgacgatcta acccttcaag aatattctac aattgttctg tttaggaaaa gcaaggcggg
     6661 aagtcaggag ataagtcatt gataaagtgg cggagagagg gggatttgaa cccccggtag
     6721 agttgcccct actccggttt tcgagaccgg tccgttcagc cgctccggca tctctccgtt
     6781 cagatggttg ccatgatgcc aggaaatttg gcattttaac agtccctgtt cgtgcaattt
     6841 tgttcaagtg acgagtttgc gagcaaaacg atgattaagt ggccctggaa agtacaagaa
     6901 tcagcacatc aaactgccct tccctggcag gaagcactat cgatccccct tttaacgggt
     6961 ctgacggaac aggaacaaag caaattagtc actcttgccg aacgtttttt acagcaaaag
     7021 cggcttgttc ctttacaggg ctttgaactg gattcattaa gaagctgccg gatagcactt
     7081 ctattttgcc tacccgttct ggagttagga ctggaatggc tggatggttt tcatgaagtc
     7141 ttaatttatc ctgcgccatt tgtggtcgat gatgaatggg aagacgatat cggtctggtg
     7201 cataaccaac gtattgttca gtcaggtcag agctggcagc aagggcctat cgttttgaac
     7261 tggttggata tacaagattc ttttgatgca tctggtttta acctgattat tcatgaagtc
     7321 gctcataagc tggacacccg taacggcgat cgcgccagcg gagttccctt tattccgttg
     7381 cgtgaggttg ctggctggga acacgatctt catgctgcaa tgaacaacat tcaggaagaa
     7441 atcgaattgg ttggcgagaa tgcggcgagc attgatgctt atgcagccag cgatcctgct
     7501 gaatgttttg ccgtactttc tgaatatttc tttagcgccc cagaactttt tgctcctcgt
     7561 ttcccttcat tgtggcaacg tttctgccaa ttttatcaac aagatccttt gcagagactg
     7621 catcacgcta atgatacaga ctcgttttcg gcgacgaatg ttcattaatt aacaactttg
     7681 cagattaatt aaccaattga aatgacttat gaaatttagt gttgacagac aaggtaccgc
     7741 taagtaatat gtgccccgtt cacacgattc ctctgtagtt cagtcggtag aacggcggac
     7801 tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaattct aaaaattcgc
     7861 ttttttagcg caatgtcact gaccttagtt gaacattgtt ttttaacgga tagcgggttt
     7921 ttaacacctt aagcgccctc gacctttatg gttgagggcg ttttgctatg aacgccatca
     7981 ccattttccc ctcgattata aaacttgagt tattcagtag tctcccctct tgcaactcac
     8041 acccaaaact gcctaacgaa aagttattaa ttttcaatcg tattgctatc agtatttaca
     8101 ttttttcgct gtgctagaaa gggcgcattt atgttagctc gttcagggaa ggtaagcatg
     8161 gctacgaaga agagaagtgg agaagaaata aatgaccgac aaatattatg cgggatggga
     8221 attaaactac gccgcttaac tgcgggtatc tgtctgataa ctcaacttgc gttccctatg
     8281 gctgcggcag cacaaggtgt ggtaaacgcc gcaacccaac aaccagttcc tgcacaaatt
     8341 gccattgcaa atgccaatac ggtgccctac acccttggag cgctggaatc ggcccaaagc
     8401 gttgccgaac gtttcggtat ttcggtggct gagttacgca aactcaacca gtttcgtacg
     8461 tttgctcgag gttttgataa tgtccgccag ggtgatgaac tggatgtccc ggcacaagtt
     8521 agtgaaaaaa aattaacccc gccgccgggt aatagcagtg acaacctcga gcaacagata
     8581 gccagtactt cacagcaaat cgggtctctg ctcgccgaag atatgaacag cgagcaagcg
     8641 gcaaatatgg cgcgtggatg ggcctcttct caggcttcag gcgcaatgac agactggtta
     8701 agccgcttcg gtaccgcaag aatcacgctg ggcgtggatg aagattttag cctgaagaac
     8761 tcccagttcg attttctcca tccgtggtat gaaacgcctg ataatctctt tttcagtcag
     8821 catactctcc atcgtactga cgagcgtacg cagattaaca acggcttagg ttggcgtcat
     8881 ttcactccca catggatgtc gggcatcaac ttctttttcg accacgatct tagccgttac
     8941 cactcccgcg tcggcattgg cgcggagtac tggcgcgact atctaaaatt aagcagtaac
     9001 ggctatttgc gactgaccaa ctggcgcagc gcacctgaac tggacaacga ttataaagca
     9061 cgcccggcca atggctggga tgtacgcgca gaaagctggc tacccgcctg gccgcacctt
     9121 ggcggtaaac tggtctatga acagtattat ggcgattaag tggccctgtt cgataaagac
     9181 gatcggcaaa gtaatcctca tgccataacc gctggactta actatacccc cttcccgctg
     9241 atgaccttca gcgcggagca acgccagggt aaacagggcg aaaatgacac ccgttttgcc
     9301 gtcgatttta cctggcaacc tggcagcgca atgcagaaac agcttgaccc gaatgaagtc
     9361 gctgcacggc gtagccttgc aggcagccgt tatgatctgg tggatcgcaa caacaatatc
     9421 gttctggaat atcgcaaaaa agaactggtt cgcctaaccc tgacagaccc cgtgacaggg
     9481 aagtcaggag aagtgaaatc actggtttcg tcgctacaaa ccaaatatgc cctgaaaggc
     9541 tataacgtcg aagccaccgc actggaagct gccggtggca aagtggtcac aacgggtaaa
     9601 gatattctag ttaccctgcc ggcttaccgg ttcaccagta cgccagaaac cgataacacc
     9661 tggccgattg aagtcaccgc cgaagatgtc aaaggcaatt tgtcgaatcg tgaacagagc
     9721 atggtggtcg ttcaggcacc tacgctaagc cagaaagatt cctcggtatc gttaagtacc
     9781 caaacattga acgcggattc ccattcaacc gccacactga cttttattgc gcatgatgca
     9841 gcaggtaatc ctgttgtcgg gctggtgctc tcgacgcgtc acgaaggtgt tcaggacatc
     9901 accctttctg actggaaaga taatggtgac ggaagctata cccagatcct gaccacagga
     9961 gcgatgtctg gcacgctgac gctgatgcca cagctgaatg gtgtggatgc ggctaaagcc
    10021 cccgccgtgg tgaatatcat ttctgtttcg tcatcccgaa ctcactcgtc aattaagatt
    10081 gataaggacc gttatctctc cggcaatcct atcgaggtga cggtagaact gagagatgaa
    10141 aatgacaaac ctgttaagga acaaaaacag caactgaata acgcagtcag catcgacaac
    10201 gtgaaaccag gagtcactac agactggaaa gaaaccgcag atggcgtcta taaggcgacc
    10261 tataccgcct ataccaaagg cagtggactt actgcgaagc tattggaatg aagatttgca
    10321 taccgctgga tttatcatcg acgccaaccc gcagtcagcg aaaattgcga cattatctgc
    10381 cagcaataat ggtgtactcg ccaatgagaa tgcagcaaac accgtctcgg tcaatgtcgc
    10441 tgatgaagga agcaacccaa tcaatgatca taccgtcacg tttgcggtat taagcggatc
    10501 ggcaacttcc ttcaacaatc aaaacaccgc aaaaacggat gttaatggtc tggcgacttt
    10561 tgatctgaaa agtagtaagc aggaagacaa cacggttgaa gtcacccttg aaaatggcgt
    10621 gaaacaaacg ttaatcgtca gttttgtcgg cgactcgagt accgcgcagg ttgatctgca
    10681 gaagtcgaaa aatgaagtgg ttgctgacgg caatgacagc gccacaatga ccgcgaccgt
    10741 ccgggatgca aaaggcaacc tgctcaatga cgtcatggtc actttcaatg ttaattcagc
    10801 agaggcgaaa ctgagccaaa ccgaagtgaa tagccacgac gggatcgcca cagctacgct
    10861 gaccagtttg aaaaatggtg attatagggt tacggcctct gtgagctctg gttcccaggc
    10921 taatcaacag gtgaatttta tcggtgatca aagtactgct gccctgaccc tcagtgtgcc
    10981 ttcaggtgat atcaccgtca ccaacacagc tccgcaacat atgactgcaa ccttgcagga
    11041 taaaaatggc aacccactaa aagataaaga aatcaccttc tctgtgccaa acgacgtcgc
    11101 aagtaagttc tcgattagca acggaggaaa aggcatgacg gatagtaacg gggttgcaat
    11161 cgcctccctg accggcacgt tagcgggcac gcatatgatc atggctcgtc tggctaacag
    11221 caatgtcagc gatgcacagc caatgacgtt tgtggcggat aaagacagag cggttgtcgt
    11281 tttgcaaaca tcgaaagcgg aaatcattgg gaatggcgtg gatgagacaa ctctgacagc
    11341 aacagtgaaa gatccgtcga atcatccggt ggcggggata acggtaaact tcaccatgcc
    11401 acaggacgtt gcggcaaact ttacccttga aaataacggt attgccatca ctcaggccaa
    11461 tggggaagcg catgtcacgc tgaaaggtaa aaaagcgggc acgcatacgg ttaccgcaac
    11521 gctgggtaat aacaatacca gtgattcgca gccggtaaca tttgtggcgg acaaagcctc
    11581 ggctcaggtt gtcctgcaga tatcaaaaga tgagatcaca ggtaatggcg tcgatagcgc
    11641 aacgctaact gcaacggtta aagatcagtt cgacaatgag gtgaataatc ttccggtaac
    11701 attcagctca gcctcttcag gactcaccct gaccccggga gtaagtaata ccaatgagtc
    11761 tggcatcgcg caggccactc tcgcaggcgt tgcctttggt gagcagacgg tcactgcatc
    11821 actggctaat aatggtgcca gcgacaacaa aactgtgcat tttattggcg acacagcggc
    11881 ggcaaaaatt atcgagttga cgcctgtccc agacagcata atcgccggta ccccgcagaa
    11941 cagctccggc agcgccatca ccgccacagt cgttgataat aatggctttc cggtgaaagg
    12001 tgtgactgtg aacttcacca gcagaacaaa ctctgccgaa atgacgaatg gcggccaagc
    12061 cgtaacgaac gaacagggta aggctaccgt cacttatacc aatacccgct cctcgataga
    12121 atcaggagcg agaccggata ccgttgaggc cagtctggaa aatggtagct ccacgcttag
    12181 cacatcaatt aatgtcaacg ctgatgcgtc tacggcacat ctcacctggc tacaggcact
    12241 ttttgataca gtctccgcag gcgagacaac cagtctgtat attgaggtga aggataatta
    12301 cggcaacggt gtcccccagc atcaggtgac cctcagcgtt tcaccaagtg aaggcgtgac
    12361 ccccagtaat aacgctatat atactaccaa ccacgacggc aatttttacg caagctttac
    12421 cgctacaaaa gccggggttt atcaagtgac ggcaaccctc gaaaatggcg attcgatgca
    12481 acaaacagtg acctatgtgc cgaacgtcac gaatgccgaa atcacgctgg caacctcgaa
    12541 ggatccggtg attgccgaca ataacgatct cacgacacta acagcaacag tcgctgatac
    12601 agagggcaat gcgatagcca acactgaagt aacatttact ctgccggaag atgttaaggc
    12661 gaacttcacg ctgagcgatg gcggtaaagc gattactgat gctgaaggca aagcgaaagt
    12721 cacgctgaaa ggtacaaaag caggcgctca tactgttaca gcatcgataa ctggcggtaa
    12781 gagtgagcag ttggtggtga actttactgc ggatacgctc actgcgcagg ttaatcttaa
    12841 cgttaccgag gacaatttta tcgctaataa cgtcgggatg accaggctgc aggcaacagt
    12901 gactgatgga aacggcaacc cgttagccaa tgaggcggtg acattcacgc taccggcaga
    12961 tgtgagcgca agctttactc tcggacaagg cggttccgcc attactgaca tcaacggcaa
    13021 ggctgaagtt acactgagcg gtacaaaatc cggcacctac cccgtgacag ttagcgtgaa
    13081 caattatggt gtcagtgata cgaaacaggt gactttgatt gccgatgctg gtaccgcaac
    13141 actagcctcc ttaacctctg tatactcatt cgtcgtcagc acgaccgaag gcgcgaccat
    13201 gactgcaagc gtcactgacg ctaacggcaa cccggtagaa ggcataaaag ttaatttccg
    13261 cggaacctcc gtcacgctaa gcagcaccag cgttgaaacg gatgatcggg gtttcactga
    13321 aattcttgtg acaagcaccg aagtcggact gaaaacagtt tcagcctctc tggcagataa
    13381 acctactgaa gtcatatcgc gattactgaa tgcaaaagca gatattaatt ctgcaacgat
    13441 taccagtctg gagatacctg aaggtcaggt catggtcgca caagacgtag cagttaaagc
    13501 tcacgtcaac gaccagtttg gcaacccgat tcttaatgaa tctgtaacat tcagtgcaga
    13561 gccaccagag cacatgacca tcagccaaaa tattgtctct actgatacgc atggtatagc
    13621 cgaggtctcc atgacgcccg aaagaaacgg ttcgtatatg gtgaaagcat ccctggcgaa
    13681 tggagcctca cttgagaaac aactggaggc tattgatgaa aaactgacac tctcggcgtc
    13741 cagcccgctt atcggtgtca attccccaac aggtgcaact ctgacggcaa cgctaacttc
    13801 tgcaaatggc actccagtgg agggtcaggt catcaacttt agcgtaacgc cagaaggtgc
    13861 gacgttaagt ggcggaaaag tgagaactaa ctcttctggt caggctccag tcgttctgac
    13921 cagcaataaa gtcggtacat atacggtgac tgcatcgttc cataacggcg taacaataca
    13981 gacacagaca accgtgaaag tcactggcaa ctcaagcacc gcccatgtta ctagctttat
    14041 cgctgatcca tcgactatcg ccgccaccaa cagtgattta agtaccttaa aggcaacggt
    14101 tgaggatg
//
LOCUS       DAETQN010000111        14091 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.111, whole genome shotgun sequence.
ACCESSION   DAETQN010000111 DAETQN010000000
VERSION     DAETQN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.111"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="K0W68_003296"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="K0W68_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBI3074770.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="K0W68_003297"
     CDS             2273..2662
                     /locus_tag="K0W68_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBI3074771.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="K0W68_003298"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="K0W68_003298"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBI3074772.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="K0W68_003299"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="K0W68_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBI3074773.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="K0W68_003300"
     CDS             complement(4321..4521)
                     /locus_tag="K0W68_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBI3074774.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="K0W68_003301"
     CDS             4687..5232
                     /locus_tag="K0W68_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBI3074775.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="K0W68_003302"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="K0W68_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="K0W68_003303"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="K0W68_003303"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBI3074776.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="K0W68_003304"
     CDS             complement(6529..7353)
                     /locus_tag="K0W68_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HBI3074777.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="K0W68_003305"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="K0W68_003305"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBI3074778.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="K0W68_003306"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="K0W68_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBI3074779.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="K0W68_003307"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="K0W68_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBI3074780.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="K0W68_003308"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="K0W68_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBI3074781.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /gene="metI"
                     /locus_tag="K0W68_003309"
     CDS             complement(11316..11969)
                     /gene="metI"
                     /locus_tag="K0W68_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBI3074782.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="K0W68_003310"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="K0W68_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBI3074783.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="K0W68_003311"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="K0W68_003311"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBI3074784.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3433 a         3562 c         3737 g         3359 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagatggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    14041 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg a
//
LOCUS       DAETQN010000112        14013 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.112, whole genome shotgun sequence.
ACCESSION   DAETQN010000112 DAETQN010000000
VERSION     DAETQN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.112"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..593)
                     /locus_tag="K0W68_003312"
                     /pseudo
     CDS             complement(1..593)
                     /locus_tag="K0W68_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(750..2930)
                     /locus_tag="K0W68_003313"
     CDS             complement(750..2930)
                     /locus_tag="K0W68_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBI3074785.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            complement(3029..3235)
                     /locus_tag="K0W68_003314"
     CDS             complement(3029..3235)
                     /locus_tag="K0W68_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074786.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            complement(3260..3970)
                     /locus_tag="K0W68_003315"
     CDS             complement(3260..3970)
                     /locus_tag="K0W68_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3074787.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            complement(4446..4751)
                     /locus_tag="K0W68_003316"
     CDS             complement(4446..4751)
                     /locus_tag="K0W68_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074788.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            complement(4898..5113)
                     /locus_tag="K0W68_003317"
     CDS             complement(4898..5113)
                     /locus_tag="K0W68_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074789.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            complement(5334..5624)
                     /locus_tag="K0W68_003318"
     CDS             complement(5334..5624)
                     /locus_tag="K0W68_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074790.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            complement(5948..6244)
                     /locus_tag="K0W68_003319"
     CDS             complement(5948..6244)
                     /locus_tag="K0W68_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074791.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            complement(6244..7197)
                     /locus_tag="K0W68_003320"
     CDS             complement(6244..7197)
                     /locus_tag="K0W68_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBI3074792.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEEELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            complement(7580..8242)
                     /locus_tag="K0W68_003321"
     CDS             complement(7580..8242)
                     /locus_tag="K0W68_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBI3074793.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCNLPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNGLTIRSPFPSAVVVFKKEPRKHEITQTVCCGD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKCIADALRMPLSTSYRIAQR
                     LS"
     gene            8758..9327
                     /locus_tag="K0W68_003322"
     CDS             8758..9327
                     /locus_tag="K0W68_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3074794.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            10055..10228
                     /locus_tag="K0W68_003323"
     CDS             10055..10228
                     /locus_tag="K0W68_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBI3074795.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(10486..11814)
                     /gene="rutG"
                     /locus_tag="K0W68_003324"
     CDS             complement(10486..11814)
                     /gene="rutG"
                     /locus_tag="K0W68_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBI3074796.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(11835..12329)
                     /gene="rutF"
                     /locus_tag="K0W68_003325"
     CDS             complement(11835..12329)
                     /gene="rutF"
                     /locus_tag="K0W68_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBI3074797.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(12340..12930)
                     /locus_tag="K0W68_003326"
     CDS             complement(12340..12930)
                     /locus_tag="K0W68_003326"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBI3074798.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(12940..13740)
                     /gene="rutD"
                     /locus_tag="K0W68_003327"
     CDS             complement(12940..13740)
                     /gene="rutD"
                     /locus_tag="K0W68_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBI3074799.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(13748..>14013)
                     /locus_tag="K0W68_003328"
     CDS             complement(13748..>14013)
                     /locus_tag="K0W68_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBI3074800.1"
                     /translation="VLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKN
                     YAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         3844 a         3255 c         2896 g         4018 t
ORIGIN      
        1 tccaagcctt attacattta atctgagcat cgagaatatc atttctgata gctggagcgt
       61 aaccggatac atcgctaata ttcattaact ctttgcacga tagcataggc gcgttattgc
      121 gttcagtggt cacgttacct gcattcttct tactggtcac accaccgtca tttgtggtag
      181 ccgttttgcc agtggctgct gaaatatcag tttgacctac gattgcttta aagttaattt
      241 cctgaaaagt tagctggata cgaagaccgt tagagatagt aacatcttta gaggctttaa
      301 agttggtaat aatggaatcg tcaattttaa ttccatcttt acagataaca gaaataatct
      361 gcttctggtc acgccagttt tccagagtgt caatgaagtt ttctactaat tgaccctgac
      421 gagttaataa taagcttcct tcataattgc atgttctgtg tagtcacctg catagggcta
      481 tcaaattcca tgttttcagt tgctgataag gttaagaagg catctacatt gtctcttaag
      541 tggaaataga tgccatcttt gccactgtaa cgaagctgat tagtgtaaat cattaaattc
      601 tcctttgatt tcaaaggctt agtgtatttt aatcattttt aaccaagcct ttgatatata
      661 tgtattttct ctgttaagcg tgagctacgt gaaaaataat ttttggtgtg ggtattaact
      721 ttgctttatt cacgtagaac accgttaaat cattcttcct ctggatagct tgaacttaag
      781 agcatatcct gcgtgaactg gtcattagct gattgattga aagtaaagaa agatggttca
      841 tctggtcgaa cgacaacttg accaccaagt gaaactttca ctggctcctg aatacgctta
      901 ctcaaagcac taatttcatc cctcagaccc tgaatatctg cactcgttaa tgctgaatct
      961 tgcttaatgt tgtttaactc tggatgattg aatcttgcaa tattcattgt ggcattgtta
     1021 aacaattcac ccattgaact aaaaattcta ctccatcctt cgttaatcat attcggttta
     1081 ttagggtcac cagaatacac gggattaaag tcactaccca cacctacccc agacggatgc
     1141 atattagcgt ttttgactac ttcctgattc ataaaatcca gtgctttctt caatgcatat
     1201 aaaccaagag tgacgcctct gatttgtgga atcattgaag ctaatgctaa cgctgtttca
     1261 taaccgtcat cagcataacc aattgcaaga gcactaccta caccaccagc tacacctttc
     1321 agttttccac cacctttacc agtagctacc tgaccagcca cctgattagc tcttgtacct
     1381 aacgttgtga ttgctcttgt caggttcata acccctgtag tagcactgaa tagcgtcttt
     1441 gcagataacg ctatagcaag acctttgacg aagttgataa aaccatcacc gacttcatca
     1501 agtagttttc gtttagtgtc atcaaggctg taatagaact tcttagtctg taaaatccag
     1561 ccttcaatgg tgttattgaa tacgcgaata tcatctacac cagctttaag catgtcgata
     1621 agtccaccga tagcatcacc agcgatagaa gaactcatcc ccatttcact aagcaggtta
     1681 ctcaggctgg ataagaagcc gtttagctca tcctgtgaac ctgagaagat tttcaccatc
     1741 gtatcatccc aattgtttgc taacttacct aaagctgtct gagtgctctt agtgacttca
     1801 gcccagccac ccgctttatc aatcatcaag cccatctgtt tagcgacttt agggagagta
     1861 tcagcggaaa gtaccttccc gtttttcatg gctgcatcaa gctctgtagc agtcataccc
     1921 aatgcttcag cgaatagctt aaatgcacca gccatacggt cacctaactg cccacgtaat
     1981 tcctcagcct gaatcttgcc cttgctcgcc atctgctcaa atgctctcaa aatgccgttt
     2041 agctcatcgt tagaaagccc cattaagcga ctcatagccg ttgcattacc aaatagctcc
     2101 tgtgtctgtt cctgtgacat agaaccctga gcacctgctg caaacttagc gtagcttgat
     2161 agcccttcag agatattaag acctgtcttg tctgctaaat ttcgaataaa tacctcagct
     2221 tcagatgctt tatcaccaag tacaaactta gcggctctct gtgcggaatc tcgtttaaga
     2281 cctgcattca taatggcctt gtaagcgtta agagcaccca tcacagagta aaatgcacca
     2341 gataatttaa tcgcattgct tgcaagatta ttaaatgcgt cgcttgtatt gttaatgctg
     2401 gcggtctttg tctgtgacct gagtttattc aacgtttcac gaagagattg atttaatttt
     2461 gcaagttcag cgttaaactt ttcacttcca atattaccag ccttaccgct taagctgtaa
     2521 tactgtgctt taaacatttt agccatcagc ggattaatat caccaagctt agacatattc
     2581 acgctatgcg ctgctgtgat tgctctgttt ccgctaattc gtggttgcct tgattgtttt
     2641 ggaggattaa cagtgtcatt aaatctggtg gcaaactgaa atgcttttgt gcttcttgct
     2701 ctctgctcaa tcttgtcaaa ggtcttatta aacttccttt caagctgccc taaagaactt
     2761 gtcagattgc tgtcaagttg cttggtatcg accttaaacg ctctattcaa attgcgttca
     2821 attctttgtg ctgactgcaa agctgccttt tcaactcttt gcaagccctt aataacgttt
     2881 tcactgaaac taagttcaac gataaaacta tcaactgtga atttgctcat tcatgtttca
     2941 cctctttaag tgttcatata cgaaaaaggg aaagactacc gatatcactc gataatctct
     3001 cccgtagatt ttcacccaac caatcttgtt acctaaccag ataataatta ccatcttcac
     3061 caacaatctg acaatcactt ggtaatttat caagacacca cttagctatt ttcctgattc
     3121 tattcttttg cttagaggag gtgaatgatt tgattcgttt tggtttgttg ggttgttctt
     3181 tcaatcgttc actaattgct ttaataagct catagcgttc tgttaaatca atcattcacc
     3241 accacctttt aattattaat taaatagcct ttgcatattt gctagaaatc ttcgtaagct
     3301 gctcaatatc agcctgcata gcaatcttct tagcatcttc atattctttc acaagctctt
     3361 tcatagcctg aagctctctt gagagataca acttaacatt atcaagttgc ttgttacgtg
     3421 atgccaagat gttctgtcta gcaatttcag cgagttcttc atcgctctta ttagtcatgt
     3481 aaataaactg accagcacct cttgatgcct taaccatctt cacaattcga tgtccagcag
     3541 gaagactact aatcacggct gcgacgtttt cttgaaaagt accaaaacca tcattatctt
     3601 cgtattggta atagacaaat ggtttctgtg acaattcatc ctgaatctca aattctgacg
     3661 gctcaatagc tgattgcatc aaggtaactt tgctgtaagc gtcatctaat ctaaggcgct
     3721 gttctttcag cagttggtaa tatttctttt caagctcaag taatgcttca ctcttgcaaa
     3781 tatcaggcat ctcttgaggc tcaggagaca caaagatggt tgtttttggt gacatcttta
     3841 gcgattgctc aagctcaata cgcttgtcat gctctttacg ctgaatagtg atagctggat
     3901 gttcagtctg tggcgaccag tcactgaagc tatcaatcac tttgccatta atgtaaatat
     3961 catttgtcat tgcttttcct cttctaagcg gaagtgtttc agtttttaaa aagcacacct
     4021 gacagcctat ctaaacttag aagagactta aaaggcggtt tgccaagtat gctttttaaa
     4081 agctgaatag ccctagataa tttcttataa agggccattc ataaggtgaa gaaattaaaa
     4141 catctaaagg attcaaaaag gattcaaacg aatgaacaag agtacttttc gagatgacct
     4201 ctcagactaa cctgaaaggt catgtagcga aaagcattgc acctttcaga ataactatca
     4261 ttgatgtaga atgattaata gtttaattca ggaagagttg ctcttcccta ttaccatcat
     4321 ctcatgctta aggttatttg tcaacagctt tttaacagat tggtaacatc aattcttatt
     4381 taccgtatta cctacccagc aaccaacaaa taaccctaaa gtcatccaga gaataacgag
     4441 tgtcattaca tcacctcgct gtaagggttt aattcattct gataggtcaa gaatacattc
     4501 agcttcaata attgctcagg gcttaaatca agtcgattgc actcttcaat gataaagcgc
     4561 ttattgtatg ggtctgttac ttccagcttg tgaacatagt caccaagttt aatttcgttg
     4621 attatatgct tgtcaatata cgttgacgct tcaaaacaat gcatgaaata gatattaaag
     4681 cccgacctgt tcaggtcaat caaccaattc atggtttctt ggtcaaccat aatttgcatt
     4741 gggtaattca tttttaaaat ccttcctgta tcgcattcta agacgtttta accccttttt
     4801 tagaccactt aaggcgattc cctaagttac cctgattagg ggcttaaaaa cgtttctggt
     4861 gcgtctggta aagcctgaga gatatgtttt gctgttttta gctcaagtct gagactaata
     4921 gccccatctg gtgaccgtag tcttttaacc agctttgctt aaccggagtc ttcagagaat
     4981 agttaggggc tgacaagtga agatagatat catctttgcc gtggatactt ccccatcctt
     5041 cgtataactc aagcaagttt tcaaaataac gaaatgcttt attagtctga agttgtactg
     5101 tgatagtttt catcaagcac cccattcaga aataagctta attccctgct caagctgttc
     5161 agggtcgtta tagtcgagta ttaccagtgg ctttaattcg tgtttaagct ggtggatttg
     5221 agagggtttt aatcgtttgt ttgctttgag ggtcacatct tcaaattcag catctgctaa
     5281 attctgtctg attaggtggc ggccaagtgt ggaatattgg aacctataac acattaaacc
     5341 tccaatctat ccgccagttg atggacctta gcgcttaaca cctgaatcat tgagcaacct
     5401 gctactgcgg actgtgcttt gagtagttcc agttggcccg ttgtgattct gatggcctta
     5461 gaaccctctg cgacgccctt aacgttatcg aaagggctaa ggtttaccga ctcttttaaa
     5521 atctccccaa caagcgctgt gtgcttcata tcgggatatt tggctttgat tgctgccagt
     5581 cgttcaaatg cctctggcaa aaggcgcgta actgtgtgag tcattgtaaa aatctcctta
     5641 aatctcttac tcatactaca atcttaacat ctatttaaca tattgtcagt acctcattta
     5701 catgatacta tggttgtagg ggctgataca ggtgctgttt cctttcacaa aaggaaaatt
     5761 aatgcatgat tttttcacag caattattga atctcatcgt tttagctcgt tgggctttgc
     5821 ggtatttatt gtgctattgg tgttttgcgt tatgaatcga gtcattacaa aaaagacaaa
     5881 gcttacaatt cgacttaaac ttcttcgtgt tgagatagag atttcagtaa ataaatagct
     5941 ggtggcctta ccggcacttt cccttgataa tattggtggc cctcagatta ccggggtcac
     6001 cgtcttttaa tcgtagctgg tggactgaat cccataaatc gtaatgatac agcaccgcaa
     6061 acttcaaagc gtcaatcttt aaatctgtca catcatcgac gaaataataa tccctccctt
     6121 ctttcttcgg tcgaatccgg taaacttccc cgtgagtatc tttcatcaag taaccttcac
     6181 gagaaatgct gtaacgtctg agtaaatagt cacggtattt tctctcgcca ttataagttt
     6241 tcattaaaac attcctccac cataaacacg tctgacttta actttttctt tctttggctc
     6301 ggtgtgagtt ttcttttttg gtgtaaaagt aaaatcatcg tcactatcat caaagtcatc
     6361 gaggtcacta accttgttct ttagcttcgc atttctgaat ctttctttaa tttctgcttt
     6421 acgctgttca cgtttcaatt cctcttcagg gtcttcttca tcatcaaggt caacatccgg
     6481 taattcatca tcagtggcgg ggcactgtgt gtttttatgg tcactactag caatcacatc
     6541 agagatttta tcttcctttt taacgacctc tttagcagct tcactatcaa ggtcatctaa
     6601 gtcagatgtt ttaacagtct ctttaatagc ctcttcaata ccaagagaat cacattcatg
     6661 tgaatcacag agcgaagctc ccttcttaaa atattcttct tcaaagtcat cttctttatt
     6721 gttattaata agttctttct tgttattact gttattatac gtgtcaaaga ttttgactga
     6781 ctgagtaaaa aattttgact cagtgggtaa agaattttga ctcagtgggt catctttctt
     6841 gactgactga gtaactttag actccagctt tgcctttctg gcttccttag ctgtaagctc
     6901 tgttaaaacc tccggtactt catcaagtga ataaacttta ataacacact gattgcttgc
     6961 aacgttagtt ttcttgatta aaccatgtgc ctcaagttgt ttcatacatg caatcacgga
     7021 tgttcttgtg attccagcat actgaccaag atgagcatat gaaggggtat aatattgccc
     7081 cttatcgtga taaaactttt gttcattcaa catgacagtg taaataatac ggtcggcagc
     7141 tttaacgcca ttagtcatct gataaatacg gtcatcaaac atgtggaatt tttccattat
     7201 attttccttt ttaaaaataa aacaaagaaa gggctgacca cgtatagtga ccaaccccag
     7261 agggagtgaa ttaattagaa gatggactga taaaggaact cagaagcctg agcgattacg
     7321 cgagtattaa cagcaaaagg aaaagccttg attgaataag aggccgtgaa aatctgtcaa
     7381 taccctgacg cctcccaacg ttgtaacctc ttaatgttct gagttctttt atcaccccat
     7441 ataaaaacta taacagatgt gattataaat tgcaacaatt atttcacaga ataattactg
     7501 attggtcatt tattgttcac agggatactt cttaatatct gaatcaccta tcttgtaaat
     7561 gaccatattt atgagaattt tagctcaacc tttgagcaat cctgtatgac gtgctcagag
     7621 gcattctgag agcatctgca atgcacttaa tgggttttcc ttgcctgtat agttttatga
     7681 agctgtcgag cgtctcaggt gggtaaatgg tgggtcttcc tctcaagcct cctctgttaa
     7741 tgatggttaa cggtaatagc gtgtcaccac agcaaaccgt ctgtgtaatt tcgtgcttac
     7801 gtggttcttt tttaaaaaca acgactgcac taggaaacgg tgaacgaata gtaagcccgt
     7861 tatggcctgc tggtggaagg tatttaactg cacgatgaag aatacggatt tcatgacagt
     7921 ggttattgct gtaaatgtgc ctaagccagt agctggtgtt ggctctatgc ggtatcagga
     7981 aagctactaa atccgcttca gacgctttta agaggaacga cggaatatcg ctgtacggca
     8041 ggttacagaa gaccttttca ctaacccatg attgacggct tatatcattg gtgaatcgtg
     8101 gtaacagcgt gtcatgctca gttgcagcac catctagcgt aaaaccaaat tcacggtgta
     8161 agttcttaaa taacaacaat ggagttctcc agtaatcaga acccccatga tgtttcccga
     8221 ttaaaatatg ctcaattgac ataagaaata aaaaatttta ttgcaatcac tcttgacaag
     8281 gtttactctc ttttaagata agagatactt aacaggcttt ctaaacggat atcctttctg
     8341 gaatatctat ctcttatctt aaaagtcttt acagatactt taaagatatc tgtattaaat
     8401 ctgtacagta acttttcaga atctgaaaat ttttataaaa tcgtagtagc ccctcttcaa
     8461 attccccttc taaaaatctt cgaaaatatc tgacagatgt ctgtagctgc taagtacccg
     8521 tcacaccagc tatgtgaccc cctttgcaaa aaagccaaat atcattgact ttctcggtga
     8581 attgctataa aggcatctat ctaggtcttt aactgtataa atacacatga caacaaacgc
     8641 ccgttaagtg tctatataaa aaggtagctg tagctataga caacaattgt gataattgat
     8701 ttaatggcta tagacaagca agacagacct ttgttgacta tatagagaga taagaacatg
     8761 cgtacattcc tttatgctcg tgtatcagtt gatgacctca ccacacagaa ccaagtagaa
     8821 gctgttaaac gtgctggtta tgaagtcaga ccttcacgag taattgagga agttattagt
     8881 ggggctacac cagctatgga cagaccacaa tttgtaaagc tggtggataa gctggaagaa
     8941 ggtgattgtc ttgtagtgac caagattgac cgcttaggaa gggataacat tgacgtacag
     9001 aagaccgtca caatgttact tggtatgggt gttaagctgg tctgtctaga tttgcctgtc
     9061 caagaccttt caagtgctga aggtaaactt attcttcaac tattcagtac ctttgcagag
     9121 tttgaactta accgcatcag agagcgaacc agagagggct tagaacgagc taaagcacaa
     9181 ggtaaggtgt tgggtcgtcc tgtagctaaa aacactacag aggccgtttt agagcataag
     9241 gctaagggat tgtcacaaag tcaggttgca aagcttctga atctctctgt gagaacggtt
     9301 agccgtcact ggacaaagac actgtaaaaa aaacttagta gcatcttcat agcctccttc
     9361 atgggggctt ttttgtgtct gtaacatatg gtgaccaaac atctatatat cgttatatag
     9421 ccctgtatat agcactatat atagtgtctg gagagggacg actaacaccc tgcatagttc
     9481 cctgaaaagg ggcttcacag attctctata tatttcaata agttaggata aaaagggact
     9541 ataccgatac tgcatagaat ctgtgtagga ttgcatagtc ataggggttg gaacaagccc
     9601 aactacacct gataaagcct gttattaaca ggtagttgaa tgtacgtgtt tagctatgta
     9661 aaagcaccag aactttagcc atttctaacc actcctcgtg ttatctctat tccgtagcga
     9721 ttcgctacca cttatttaaa gataagacgt ccttttcaga gtgcaaattt cacaaccact
     9781 tatttgattt ataacaactt tcacaagcac gtaattttgt cgcaaaatga cacattttta
     9841 tctcatcgcg tttttttaat cataagagcg gcttatggat aattattgga gatgatatct
     9901 attctcgcta agaagctgtt gcaggatatt accaaacgcg ggtctacccg cgtcagttca
     9961 ctaagcttag tcccacgtag cgaaaatatg gcagccgcca tacgccgcgt taattctatg
    10021 caatatgatg tctataccca gacggaggtc agtaatggca aaccatcgag gcggttccgg
    10081 caattttgca gaagaccgcg aaagagcatc agaagcaggt aaaaaaggtg gacagcacag
    10141 cgggggtaat ttcaagaatg acccgcagcg cgcatctgaa gcaggtaaaa aaggtggcaa
    10201 gagcagtcac ggcaaaagcg acaactagcc gggctaatca atgacgaatg catttttgtc
    10261 tgtagctcgt caaaaagcca tcaccgccgg ttacccggtg gttgatactg atgacaaatg
    10321 taagcttgcc tgatgcgcga tgcttatcag gcctaccaga agattgcaat atattgaatt
    10381 tgcactgttt tgtaggccgg ataaggcgtt tacgccgcat ccggcatgaa caatgcgtac
    10441 gttgtcaaca atctgcaccg ccgggtaacc ccggcggttt tctgtttatg gctcctgatg
    10501 aacaacttct ggcggtggaa cgtcaaccaa ccttctactt aacaacgcat tgagtaaaat
    10561 cgcgccaaag gtggctgtac cgatccctcc caacgtaaaa ccgcccagcg tgagagcaaa
    10621 atcacccgcg cccagcacta aggttactgc gaccataatc aaattaccgt tctggcttaa
    10681 atcgacacgg ttttgtaccc agatccttgc gcctgcgacg gcaatcagcc cgaacacaac
    10741 aattgatgca ccaccaatca ccgcggccgg aatggtatgg atcagcgcgc caaatttcgg
    10801 cgaaaagccc aacagcatgg cgatgacggc agcagcaaca aacaccagcg tcgagtagac
    10861 ttttgtcacg gccatcacac cgatattttc agcataggtg gtgacgccgc taccgccgac
    10921 agagccggaa agcatcgttg ccagaccatc accgacgaat gctcgcccca tatacgggtc
    10981 catattgcgt ccggtcatcc cggcgaccgc cttgagatga cctaagtttt ccgccaccag
    11041 aatcaccgcc acgggcgcaa tcagcatcat tgcctgacta ttaaaagcag gagtggaaaa
    11101 atgtggcaaa ccgaaccagg cagcatggct gacgagagta aaatcgacgg cttttcccag
    11161 ccctaaaacg ttggtcatca cgccatacag cagacaggcg acaattaaac ctacgagaat
    11221 caataaccgc tggatcatgc cacgggtaaa caccgccacc agcccaatac acagcaccgt
    11281 cattaccgcc atccagctat caaaggctga agccgataca cttttcactg cgataggcgc
    11341 taagttcagg ccaatcgcca tcaccaccgc acccgtcacc accggcggca ttagtcgttc
    11401 aatccagcgt gtaccaattt tcatcaccac caggccaatg acggtataaa ccagcccaca
    11461 ggcgataatc ccgcccagtg cgatgctgat attcgggtta atgccctgac cgttaaagcc
    11521 cgtcgcggca atcaccacgc cgacaaaagc cgcgctggag ccgagataac tggggacgcg
    11581 cccgccggta ataaagaaaa acagtaacgt gccgatcccc gacattaaaa tggaaagatt
    11641 gggatccagc cccatcagaa tcggcattaa caccgtcgcg ccaaacatcg ccaccgcgtg
    11701 ttgaacgccc attactgccg tctgagcaaa cggcaatcgt tcatccggcg cgaccacgcc
    11761 gctctctgta gaggtcgatt ttaactgcca gtgaggaaaa ccgaacattg ccatcagctg
    11821 tctccttaag gaggttaaca agcagggcgc atcagcgcgt gataactgcg atcgaaccac
    11881 accagcccgt agggtgtggt gtgacgatga atcgcttcga tggcgcaaaa cagaatgtcg
    11941 tgggtgccga cgctcaccac ctggctgatg cggcagtcaa acgaaaccag agcctcttcc
    12001 agttgcgggc atccggtcac acccgtctgc cagcgggcgg cggcaaagcg gtgttccatg
    12061 ggcgttttgc cgccaaaaag atttgaaagc ggctcctgcc cggcgctcag tgtatttaca
    12121 cacagcgttc gattttcatt gaataccggc cagacggacg ccccacgatt caggcacacc
    12181 agtaatgtgg gcggcgtgtc ggtcacgctg cagacggcgc tggcggtgaa cccggcgcgc
    12241 ccggatggac cgtccgtggt gatgatattg accgccgcgc ccatacagga catcgcatcg
    12301 cgaaaggttt gttgatcaac tatgttcata gtttgctcct tacaacagcc cgcaggcttc
    12361 ttcaaaggac agacgtggca ggcgcgcaaa aagtttgctg ctatcgccat agccgatatt
    12421 aatcagcaga ttgctcttca gcgtgctgcc cgcaaaaaag gcgtcgtcaa cgtgttgacg
    12481 gtcaaaaccc gacatcgggc cggtatccag tcccagcgca cggcaagcga cgatcagata
    12541 ggccgcctgc atggagctgt tgcgaaacgc tgtttcttcg gcaagttgtg ggctggaggt
    12601 aaaccaactg cgggcatcac cgtggggaaa cagtagtggt aaccgttcat aaaattcact
    12661 gtcccaggca acgatagcgg tgacgggcgc ggtcagggtt ttttgtagat tgccgctgga
    12721 aagcgccggg tgcagacgtt cttttccttc tacagtgcgg ataaacacga tccgtgccgg
    12781 agaacagtta gctgatgtcg gcccccattt tatcggggca taaagctccc gtaacgtctc
    12841 atcgctgacg ggtgtctccc gccagccgtt gtgagtgcgg gcatcggtga acagggtgct
    12901 aagcgcacct gggctaacgg cttcgttcat agcaattcct tacagggcgg cttcacggtg
    12961 atgtaacagg ctggcaagcc cgttgagtaa cagagcatta aacgtttcgg ggtcggtcac
    13021 gttgcaggcg tgtccgccat agcgcatcac cattttctgg ctatcgggca gggcggcatg
    13081 aagctcactg gaacatgctg atggcaccag cagatcatca ctggcgcaga tgatttgcac
    13141 cgggcagcgg atgcgatccg catggtgact aaagtcagcg cgtttgaggg cgttaagtcg
    13201 acgcagtaaa ttatttttgc cctgaaaatg cgccagtgcc agcgcgtctt ctgcctcaag
    13261 gcgaggtgcg cgggccgcca tccagtcggc gggatagagg aacaacggct gcgcttccac
    13321 ccatgcctgc gcgccgccgc tatacagtaa tcgttcgcga acctgaaaac agcggcgcgt
    13381 atgggcgttt attcgtagcc agccgttaac gctgatcagc acagttaccg acgcgggata
    13441 atccagcgcc agctgcattc ccaccagcgc accgagcgca tggccgacca ccgcgtaatg
    13501 ctcaatccct gcggctacca gcgcctgatg cagttccgct gccatctggg cgatactgta
    13561 atcttctgcc agcgtgtcgg gattattgcc ggtgccgcgc tggtcgtaac agactacctg
    13621 atactcctgc tccagcaccg ccagttgcgg taaccagtaa ctgccgctac cgccaagacc
    13681 cgaaatcaac accactacgg gcgcatcagc ataaggggga ggtgagagtg aaagtttcat
    13741 cgcggcctca cttggcgata tgcgcaattg tggcgatttc caccagcgcg tcaggtttta
    13801 ccagtccgca ctgaatgcag aatcgcgccg gtttatcacc cggaaaaaac tcggcgtaga
    13861 tttcgttaat cgcggcgtaa tttttccagt cggtaataaa gatgctgttg aaggtcacat
    13921 ccgccatcgt gccacccgcc gtctcgatca ccttgcggat agtttccaga acgtggcggg
    13981 tttgcgcctt tgggtcatcg gcaaacagca cgt
//
LOCUS       DAETQN010000113        13991 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.113, whole genome shotgun sequence.
ACCESSION   DAETQN010000113 DAETQN010000000
VERSION     DAETQN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13991
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.113"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1284
                     /gene="narU"
                     /locus_tag="K0W68_003329"
     CDS             <1..1284
                     /gene="narU"
                     /locus_tag="K0W68_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HBI3074801.1"
                     /translation="KTITYLSDTGCLEIQGASLAVTVNLNKIGFNFTTDQLFLLTALP
                     SVSGALLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALL
                     CGFAGANFASSMGNISFFFPKAKQGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFL
                     GVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLH
                     LWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISD
                     KFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQM
                     IAVIFRQITIYRVKMKGGSDEQAQKEAITETAAALGFISAIGAVGGFFIPQAFGMSLN
                     MTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK"
     gene            1366..5106
                     /gene="narZ"
                     /locus_tag="K0W68_003330"
     CDS             1366..5106
                     /gene="narZ"
                     /locus_tag="K0W68_003330"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HBI3074802.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5103..6647
                     /gene="narH"
                     /locus_tag="K0W68_003331"
     CDS             5103..6647
                     /gene="narH"
                     /locus_tag="K0W68_003331"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBI3074803.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6647..7342
                     /gene="narW"
                     /locus_tag="K0W68_003332"
     CDS             6647..7342
                     /gene="narW"
                     /locus_tag="K0W68_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBI3074804.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7339..8019
                     /gene="narI"
                     /locus_tag="K0W68_003333"
     CDS             7339..8019
                     /gene="narI"
                     /locus_tag="K0W68_003333"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBI3074805.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8098..8991
                     /locus_tag="K0W68_003334"
     CDS             8098..8991
                     /locus_tag="K0W68_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HBI3074806.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9087..9932)
                     /gene="nhoA"
                     /locus_tag="K0W68_003335"
     CDS             complement(9087..9932)
                     /gene="nhoA"
                     /locus_tag="K0W68_003335"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HBI3074807.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10105..10674
                     /locus_tag="K0W68_003336"
     CDS             10105..10674
                     /locus_tag="K0W68_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBI3074808.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10678..10905)
                     /gene="pptA"
                     /locus_tag="K0W68_003337"
     CDS             complement(10678..10905)
                     /gene="pptA"
                     /locus_tag="K0W68_003337"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HBI3074809.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(11005..12140)
                     /locus_tag="K0W68_003338"
                     /pseudo
     CDS             complement(11005..12140)
                     /locus_tag="K0W68_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(12280..13416)
                     /locus_tag="K0W68_003339"
     CDS             complement(12280..13416)
                     /locus_tag="K0W68_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBI3074810.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSTDD
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEKKFPLTELNNP
                     AHDSSAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMMVRYYISSADLTAEKFATAIRNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINVLTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            complement(13773..13949)
                     /locus_tag="K0W68_003340"
     CDS             complement(13773..13949)
                     /locus_tag="K0W68_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074811.1"
                     /translation="MSFIFNEETLYFTVFGGDFIMDDLKIVKNTEAWVWYKNCDSKNK
                     KVAVELLQIYELHQ"
BASE COUNT         3388 a         3544 c         3447 g         3612 t
ORIGIN      
        1 aagactatca cttatttaag tgatactggt tgtctggaga ttcagggggc cagtctagca
       61 gttaccgtta atctcaataa aatcggtttt aattttacta ccgatcaact ctttttatta
      121 accgcattac cctccgtttc tggcgcatta ttgcgtgttc cctactcctt tatggtgcct
      181 atattcggtg gacgccgatg gacggttttt agtactgcaa tcctgattat tccttgcgtc
      241 tggctcggaa ttgccgtgca aaatccgaat actccttttg ggatatttat cgttatcgct
      301 ttgctatgcg gttttgcagg tgcaaacttt gcttcgagca tgggcaatat cagtttcttc
      361 tttccaaaag ccaaacaagg gagcgctctt gggattaatg gcggattagg aaacttaggt
      421 gtaagtgtaa tgcagctggt tgcaccgctg gtcatttttg tacctgtatt tgcctttctc
      481 ggcgtcaatg gcgtaccgca ggccgacggt tcggtgatgt cgctggcgaa tgccgcatgg
      541 atttgggtac cgctactggc gattgccacg atcgccgcct ggtcagggat gaatgatatc
      601 gccagttcac gcgcctcaat tgacgaccag ctccctgtct tacaacgcct gcatctctgg
      661 ctgctgagcc tgctttacct tgccaccttc ggttcgttta tcggtttttc tgcgggtttt
      721 gccatgctgg caaaaaccca gttcccggat gtgaatattc tgcgcctggc gttctttggc
      781 ccatttatcg gtgccatcgc gcggtcggtt ggtggtgcta tttccgataa attcggcggc
      841 gtgcgggtga cgttgatcaa ctttattttt atggcgattt tcagtgccct gctgttcctt
      901 accttaccgg gcacaggctc cggtaatttc atcgcctttt acgccgtatt tatggggctg
      961 tttctgactg cgggtctggg aagtggttct actttccaga tgatcgccgt catctttcgc
     1021 cagataacca tttatcgggt gaagatgaaa ggcggtagtg atgagcaagc tcagaaagaa
     1081 gccatcaccg aaacggcggc ggctctgggc tttatctcag ccattggcgc agtgggcggc
     1141 ttttttattc cgcaggcgtt tggcatgtcg ctcaatatga ccggctctcc ggtcggcgcg
     1201 atgaaagtgt ttttaatctt ctacatcgtt tgtgtgctgc tgacctggct ggtttatggt
     1261 cggcggaagt tcagccaaaa ataagagatc gatgcctgat gtacaaccgt gtcgcatccg
     1321 acaaggcatc agatcccaat tcattcctgg agcaggagtc atgtcatgag taaacttttg
     1381 gatcgctttc gctacttcaa acaaaagggc gaaacctttg ccgatggtca cggacaggtg
     1441 atgcatagca accgcgactg ggaggacagc tatcgccagc gttggcagtt cgacaaaatt
     1501 gtgcgttcca cccacggtgt taactgtaca ggctcctgta gctggaaaat ctacgttaaa
     1561 aatggtctgg tgacctggga aatccaacag accgactacc cgcgcactcg ccctgaccag
     1621 cccaatcatg aacctcgcgg ctgcccgcgt ggcgcaagtt actcctggta tctctacagc
     1681 gctaaccgcc tgaaataccc gctcattcgt aaacgactga ttgaactgtg gcgtgaagct
     1741 ctcaagcaac acagcgatcc ggtactggcg tgggcatcga ttatgaacga tccgcaaaag
     1801 tgcttaagct acaaacaagt gcgtgggcgc ggcgggttta tccgctccaa ctggcaggaa
     1861 cttaaccaac tgattgccgc cgctaacgtc tggaccatca aaatctatgg tccggatcgc
     1921 gttgccggtt tctcgccgat cccggcgatg tcgatggtgt catacgctgc cggaacgcgt
     1981 tatctgtcgc tgttaggcgg cacctgttta agcttctacg actggtattg cgacctgccg
     2041 cccgcctcgc cgatgacctg gggcgaacaa accgacgtgc cggaatctgc cgactggtat
     2101 aactccagtt acatcatcgc ctgggggtct aacgtaccgc agacacgtac gccggacgcc
     2161 cacttcttta ccgaagtacg ttacaaaggc accaaaacta tcgccattac ccctgactac
     2221 tctgaagtgg ccaaattgtg cgaccagtgg ctggcaccga aacaaggcac tgatagcgcc
     2281 ctggcgatgg caatgggcca tgtgatttta aaagagtttc atctcgataa tcccagcgac
     2341 tactttatca actactgccg ccgctatagc gacatgccga tgctggtaat gctggaacct
     2401 cgcgacgatg gtagctacgt acccgggcgc atgatccgcg cgtctgacct ggtagatgga
     2461 ctaggcgaaa gcaataatcc gcagtggaaa accgtggcgg ttaataccgc aggtgaattg
     2521 gtagtgccga atggttcgat tggtttccgc tggggagaaa aaggcaaatg gaatctggaa
     2581 tccattgccg ccggtaagga aaccgaattg tcgttaaccc tgctcggtca acatgacgct
     2641 gttgcaggcg tggccttccc ctactttggc ggcattgaaa atccgcattt tcgcagcgta
     2701 aaacacaatc cggtgctggt gcgccaattg cccgttaaaa acctgacgtt agtcgatggc
     2761 aacacctgtc cggtggtcag cgtttatgat ttggtgctgg cgaattacgg cctcgatcgc
     2821 gggctggaag atgaaaacag cgcgaaagat tacgctgaaa tcaaaccgta caccccagcc
     2881 tggggtgagc aaattaccgg cgtaccgcgc cagtatattg aaaccatcgc ccgcgaattt
     2941 gccgataccg cccataaaac gcatggacgc tcaatgatta tcctcggcgc tggcgtcaac
     3001 cactggtatc acatggatat gaactaccgt gggatgatca atatgctgat cttctgcggt
     3061 tgtgtcgggc aaagcggcgg cggctgggcg cactatgtcg gtcaggaaaa actgcgccca
     3121 caaaccggct ggttgccgct ggcctttgcg ctcgactgga accgcccacc gcgccagatg
     3181 aacagtacct cgtttttcta caatcattcc agccaatggc gctatgaaaa agtctctgct
     3241 caggagttac tttcaccgct tgccgatgcc agtaagtaca gcggtcatct gattgatttc
     3301 aacgttcgcg ccgaacgcat gggctggcta ccttctgcgc cgcagctggg gcgtaacccg
     3361 ctcgggatta aagctgaagc cgacaaggcc ggattatccc ccacagaatt taccgcccag
     3421 gcgctgaaat cgggcgattt acgtatggcc tgcgaacaac cagatagcgg cagcaatcat
     3481 ccgcgtaatt tgtttgtctg gcgctctaac ctgcttggct cctccggcaa aggccacgag
     3541 tatatgcaga agtatctgct ggggaccgaa agcgggattc agggcgagga actcggtgcc
     3601 agcgacggga tcaaaccgga agaagtcgag tggcaaactg cggcgattga aggcaagctc
     3661 gacctgctgg tgacgctcga cttccgcatg tccagtacct gcctgttctc cgatatcgtt
     3721 ctgcccaccg ccacctggta cgaaaaagac gatatgaaca cctcggatat gcatccgttt
     3781 attcatccgc tttctgcggc ggtcgatccg gcgtgggaat cacgcagcga ctgggaaatc
     3841 tacaaaggta tcgccaaagc attttcgcaa gtgtgcgtgg gtcatcttgg caaagaaacc
     3901 gacgtggtat tacaacccct actgcatgac tctccggcag agctctcaca gccgtgtgaa
     3961 gtgctcgact ggcgcaaagg cgaatgcgat ctgattccag gcaaaaccgc gccgaatatt
     4021 gtggcggtgg agcgcgacta ccctgctacg tatgaacgct ttacctcgct cgggccattg
     4081 atggacaaac ttggcaatgg tggtaaaggg atttcgtgga atacccagga tgaaatcgat
     4141 ttcctcggca aactcaatta caccaagcgt gatggcccgg cacaggggcg tccgctgatt
     4201 gacaccgcca ttgacgcttc agaagtgatt ctggcactgg caccggaaac caacggtcat
     4261 gttgcagtca aagcgtggca ggcgctgggc gagatcaccg ggcgcgaaca tacccatctg
     4321 gcgctgcaca aagaggacga gaagattcgc tttcgcgata ttcaggcgca gccgcgtaaa
     4381 attatctcca gccccacatg gtccggtctg gaaagcgatc acgtctccta taatgcggga
     4441 tacaccaacg ttcatgagtt aattccgtgg cgcacgctgt cgggacgcca gcagctctat
     4501 caggatcatc cgtggatgcg tgcttttggt gaaagcctgg tggcttatcg cccgcctatc
     4561 gacacccgta gcgtcagtga gatgcgccag atcccgccaa acggcttccc ggaaaaagca
     4621 cttaacttcc tgacgccgca ccagaaatgg ggcattcact caacctacag tgaaaacctg
     4681 ctaatgctga cgctctctcg cggtggaccg attgtctgga tcagcgaaac cgatgcccga
     4741 gaactgacca ttgtcgataa cgactgggtg gaagtcttta acgccaacgg cgcgctgacg
     4801 gctcgtgcgg tggtcagcca acgtgtaccg ccgggtatga ccatgatgta tcacgcccag
     4861 gaacgcatta tgaatattcc tggttcggaa gtaactggca tgcgcggcgg cattcataac
     4921 tcggtcaccc gtgtttgccc gaaaccgacg catatgattg gcggttacgc gcagctggcc
     4981 tggggcttta actactacgg caccgtcggc tcgaaccgcg acgaattcat catgatccgc
     5041 aagatgaaga acgttaactg gctggatgat gaaggtcgcg atcaggtaca ggaggcgaaa
     5101 aaatgaaaat acgttcgcaa gtcggcatgg tgcttaacct cgacaaatgt atcggttgcc
     5161 atacctgttc ggtgacctgt aaaaacgtct ggaccggacg cgaaggcatg gagtacgcat
     5221 ggtttaacaa cgtcgaaacc aaaccgggca ttggttatcc gaaaaactgg gaagatcagg
     5281 aagagtggca aggcggctgg gtccgcgatg tgaatggcaa gatacgcccg cgtctgggca
     5341 gcaagatggg cgtaataacc aaaatcttcg ccaacccggt ggtgccgcag attgatgatt
     5401 actacgaacc tttcaccttc gactacgaac atttgcatag cgcaccggaa ggcaaacata
     5461 tccctactgc tcgcccgcgt tcgctgattg acggtaagcg gatggacaaa gtgatctggg
     5521 ggccaaactg ggaagaactg ctgggcggcg agtttgaaaa acgtgcccgc gaccgcaact
     5581 tcgaggccat gcaaaaggag atgtacgggc agtttaaaaa caccttcatg atgtacctgc
     5641 cgcgcctgtg cgaacactgc ctcaatccca gttgcgtggc gacctgccca agcggcgcta
     5701 tctacaaacg cgaagaagac ggcattgtgc tgattgatca ggataaatgc cgtggctggc
     5761 gtttgtgcat tagcggttgt ccgtacaaaa aaatctactt caactggaaa agcggcaagt
     5821 cagaaaaatg tatcttctgt tatccgcgaa ttgagtcagg ccaacccacc gtgtgctcag
     5881 aaacctgcgt gggtcgcatc cggtatctgg gcgtgctgct ttacgacgcc gaccgcattg
     5941 aggaagcggc gagcaccgag cgcgaagttg acctctatga acgtcagtgc gaagtgttcc
     6001 tcgatccaca cgatccctca gtgatcgagg aagccctgaa acaaggtatt ccacaaaacg
     6061 tgattgaagc tgcccagcgt tcgcctgtct acaaaatggc gatggactgg aaactggcgc
     6121 taccgctgca ccctgaatat cgcaccctgc caatggtctg gtacgttcct ccgctgtcgc
     6181 cgattcagtc ctacgcagat gcgggcggtt tgccgaaaag cgaaggcgtg ctgcccgcca
     6241 tcgaaagcct gcgtattccg gtgcaatatc tcgccaatat gttgagtgcc ggcgataccg
     6301 gtccggtact gcgggcgctg aaacggatga tggcgatgcg ccactatatg cgttcacaaa
     6361 ccgtggaagg cgttactgat actcgtgcca tcgacgaagt aggcctgagc gtcgcccagg
     6421 tcgaagaaat gtatcgctat ctcgccattg ccaactatga agatcgtttt gttatcccga
     6481 ccagccatcg ggaaatggcg ggcgatgcct tcgcagaacg caacggctgc ggttttacct
     6541 ttggcgacgg ttgccacggt tcggacagta aattcaacct gttcaacagt agccgtatcg
     6601 atgccatcaa catcaccgaa gtgcgcgaca aagcggaggg cgaataatgc agatcctcaa
     6661 agtgatcggc ctgttgatgg agtatccgga tgagctgttg tgggaatgca aggaggacgc
     6721 gctggcgttg atccgccgcg acgcgccgat gcttacggat ttcacccaca acctgcttaa
     6781 cgcgccgctg ctggataaac aggccgaatg gtgcgaagtg tttgaccgcg ggcgcaccac
     6841 gtcgctgctg ctgttcgaac atgttcatgc cgagtcccgc gatcgcggcc aggcaatggt
     6901 ggacctgctg gcggagtatg aaaaggtcgg cctgcagctg gattgtcggg aactgcccga
     6961 ttatctaccg ctgtatctgg agtatttaag cgtactgcct gacgatcagg cgaaagaagg
     7021 attgctcaat gttgcgccga tcctcgccct gcttggcggt cgcttaaaac aacgcgaggc
     7081 accgtggtac gcgttgtttg atgctctgct gcaactggcg ggaagtatac tttcaagtga
     7141 cagtgtcaca aaacaagtaa acagcgaaga gcgcgatgac acccgccagg cgcttgatgc
     7201 agtgtgggaa gaggaacagg tgaagtttat tgaagataac gccacggcgt gtgacagctc
     7261 gccgcttaat caatatcagc gacgctttag ccaggatgtc gcgccgcagt atgtcgacat
     7321 cagtgcggga ggtgggaaat gattcagtat ctgaacgtct ttttttacga tatctacccg
     7381 tacatttgtg cgacggtgtt tttcctcggc agctggctgc gctacgacta cgggcagtac
     7441 acctggcgcg cctcctcaag tcaaatgctc gataaacgcg ggatggtgat atggtcgaat
     7501 ctgttccata tcggcatttt ggggattttc ttcgggcact tgttcggcat gttaacgccg
     7561 cactggatgt acgcgtggtt tttgccagtg gcagcgaaac aactcatggc gatggtgctc
     7621 ggcggtattt gcggtgtgtt gacgttgatt ggcggcgcag gactgctgtg gcgcagtctg
     7681 acaaatcagc gcgtgcgtgc cacttccact acgccggata tcatcatcat gagcattttg
     7741 ctgatccagt gtctgttggg gctaagtacc ataccgtttt cagcacagta tcctgatggt
     7801 agcgaaatga tgaagctggt gggttgggcg caaagcattg tcactttccg tggtggatcg
     7861 tcagagatgc tcaacggcgt agcgttcgtc ttccgcctgc atctggtgtt gggaatgacc
     7921 atcttcctgc tcttcccgtt cacccgactg gtgcatgtgt ggagcgcgcc gtttgagtac
     7981 tttactcgtc gatatcaaat tgtgcgttcg cggcgataat ttcaccctgg ccggatagcc
     8041 cttcgctctc cggccacttg caggctatga tagttctcca gtcacaggag aacgttaatg
     8101 aaaccgcagg tgtaccacgt cgatgctttt acctctcaac catttcgcgg caattctgcc
     8161 ggtgttgttt tccccgccga taatcttagc gaagcacaaa tgcagcttat cgcccgcgag
     8221 ttaggccatt cggaaactgc ttttctgctg cacagcgacg acagcgatgt gcgcatccgc
     8281 tactttacgc caacggttga agtgccgatt tgcggtcacg cgacagtagc tgcgcactat
     8341 gtacgtgcga aggtgttagg tttaggaaat tgcacgatct ggcaaacatc gctggcagga
     8401 aaacatcgcg tgactatcga aaagcacaac gatgattatc gtatttcgct ggaacaaggt
     8461 acgccgggct ttgaactgcc gctggagggt gaagtacgcg cggcgattat caacgcattg
     8521 catcttactg aagacgatat tctgccgggt ttgccgatcc aggtcgcgac gaccgggcat
     8581 tctaaagtga tgatcccatt gaagccggaa gtggatatcg acgctctttc gcctgacctt
     8641 aatgcgctga ccgctatcag taaacagatt ggctgcaatg gtttcttccc gttccagatc
     8701 cgtccaggca aaaacgaaac cgatggtcgt atgttctcgc ctgcgattgg tattgtggaa
     8761 gatccggtga ctggcaatgc caatggcccg atgggtgcat ggttggtaca tcacaacgta
     8821 ttgccccacg atggcaacgt gttgtgtgtt aaaggccatc aggggcgcgc attggggcgt
     8881 gacggcatga ttgaagtgac ggtgacaatt cgtgataacc aaccggaaaa agtcaccatt
     8941 tcaggcacag cggtgatttt gtttcatgcc gaatgggcaa ttgaaatctg aaatcgttgc
     9001 ctgatgcgct tcgcctgtca ggtctacaac cgggctaatg tatgccggat aaggcgttta
     9061 cgccgcatcc ggcaacctga cataaattat tttcccgcct ccggatgcgt atcaaacgcc
     9121 gccatcacca gcgctaactc atccacagta aagccatgtt tcgcatcatc cacgcccaga
     9181 ccaaactgtt cctgcatcac ggcatataac gacgccacat ccggtagatt tcgctgctcc
     9241 accgcgtgcc cattttcata atgggtaaaa tgaaaattgg tcagtgtcag cttgccgccg
     9301 tccggcaaat ggcggcacat cagcaaatga tggcgaaaat gcgactgcgg ccagtgcgcc
     9361 gaccagaaat tgcccatcac ataatcgctt tgttgctgct cgcagagatc gaagcggtac
     9421 atcggctgcc aatgctgatg atgattaaac tgcaacaccc aatcatcacc ctcctgcaac
     9481 aaccgatact ctccgtgtgg cgtggtctgc acgagatcgg aaactaaacg aatcggcgcg
     9541 gttagcgtct gcccaccgaa accgacatca gcaatccatt tttccccttc cagttccacc
     9601 aacagcaaac gatgggtgcg cggcggtaat gctggcggat ttgataacac tacgcgccct
     9661 aacaagctgc gaacgttaaa ccccagctcg cgtaacaccc gctcaaacac gccattctgc
     9721 tcaaaacagt aaccgccacg acgggctatc accagtttct cttccagcga ttgatcatca
     9781 agctgtattt ccctcggcag caaaacgtca aggttttcaa acggaatggt gcaattgtgt
     9841 ttcaggtgca atgcacgaag cgtatcaata ttgaccgcag cagctcccga ccagttaata
     9901 cgggcaaaat agtgattcag aatgggcgtc atcaggcgtt tcccttagtg gttttctcaa
     9961 cttataacct ggaatagacg ccgcaccggc tattttgtgc gatcgcggac atgcatcacg
    10021 tttgcaaatc gccaatcccg ttaatttcgt tacttattgc gttacggact acgcttaaaa
    10081 aaaccttctt cgcgaggcga taccatgagc cgattcatcc ccatagaatt acaccatgcc
    10141 agccgtctgt taaatcatgg cccaactgtc ttgatcacca gtttcgatga gcaatcccag
    10201 cggcgtaaca ttatggctgc agcctggtca atgccggtgg agtttgaacc tccacgcgtg
    10261 gcaattgtgg tagataaatc gacatggacc agggagttga ttgaacgtaa cggtaagttt
    10321 ggcatcgtta tcccgggcgt tgcagcaact aactggacgt gggcggtggg aagtgtgtcg
    10381 gtgcgtgaag aagataaatt taattgctat ggcattccgg tggtgaaagg tccggtgctt
    10441 ggtttgccac ttgttgaaga gaaatgcctg gcgtggatgg agtgccgcct gctacctgcg
    10501 acttctgcgc aacaaaaata cgacacgctg tttggcgagg tggtatcagc agcggcagac
    10561 gcacgggtat ttgtcgaagg ccgctggcag tttgatgatg ataagctcaa tacgttgcat
    10621 catttaggtg ctggcacgtt tgttactagc ggcaagcgtg ttacggcagg ttaagcatta
    10681 catgctgtaa ccgggtttct ttatcaaagc ctccatttgg ggctcgattt cggcatccca
    10741 gatagcttgc caagattctg gttgaatctg ctgtagagca atgcttatcg agctatcttt
    10801 actgttcaga tggcgaataa taacgtcggt aatatctgca gcaagtgctg ctttttgttg
    10861 ttcgtccagt tcacgcggaa agcatttaat gtcgatgtgc ggcatgatta cgctcctttt
    10921 gaaagatgtc agtaaaatta acacacattg tgaatgtcat gtgtccggta aagcgtttat
    10981 acgacaataa tgcccagggc aagattacga aagcccgctc cccgtaagga ctgacgccag
    11041 gtagtttctg cccatggctg cttttcgcat cttacgtctt aaccctgcct tgaatacctt
    11101 atcattcgtc aaaatattaa tagcaatgtg ccgtatccct gaaaataatt ctgctgcatt
    11161 tcctcttctt attttgcagt cgtcttcatt cattaccacg tccagacgcc aatgcagctt
    11221 attctccacg tgccagtggt ttcggattgc tgtggcgaac ttctctgcgg ttaaatcagc
    11281 agaactgata taatatctga ccgtcatttc tggctctttc ttttgttctg ctattatgga
    11341 ccgaaaggag actgccacgc ataatttctt cagccctttc cattcaaacg tgaaatcaat
    11401 aagttcatca gggacatcgc aaacaatatg aagacggatt tcttctctgc cgtgactctt
    11461 ttcactaatt gcgtaactgt cattctctgg attatttaat tctttcagcg gaaatttttc
    11521 ctcaaaggct ttatttagcc gcccctggtt tccttttacc gcgaataaat aatcacctcc
    11581 ctgtttttgt atcttctctg caatatcttt ctggcaaccc atcgcatcag ttgtgatgat
    11641 ttttccttta atatccagca tgttaagaag ttcaggaatg gctgtaatct cattggattt
    11701 ctcatccgtc ttaatctgcc cgagaatcag actgtgcatt gttgagaacg cactaatgac
    11761 atgaattgct cccctgcggc actcttgtca taagagtgcc ggagcgtttt tccatcaatt
    11821 gcaatgacgt ctttatcatc tgaagaatgg cagtcacgca tccagttaat aaagcactcg
    11881 tgaaattttg caggactgat acaggataca actctggcga tggtatcgtg aacaggaata
    11941 ccattttcaa aatcaccata ttgttttaaa aaatcgagat gtgtttcccc aaaatcctct
    12001 atatcttccc agccttctgc accagaaata acggcacaaa tagtcaacag tagaatatcc
    12061 gataatttat gttccacttt ccaggcttgt ttttaatcgg ggataataga aatatgttcc
    12121 atcaattttt taagttccat tttgttctcc ttaattatgt aaggagtatt tgatcatgta
    12181 taagcaataa aaaacagctt caggtaataa ggaatatctc aatttttaaa cataaaatgc
    12241 gaattattta gtacaaaaag tgaactcctc agggcaagat tacgaaagtc cgctccccgc
    12301 aaggactgac gcgagatagt ttctgtccat ggctgctttt cgcatcttac gtcttaaccc
    12361 tgccttgaat accttatcat tcgtcaaaac attaatagca atgtgccgta tccctgaaaa
    12421 taattctgct gcatttcctc ttcttatttt gcagtcgtct tcattcatta ccacgtccag
    12481 acgccagtgc agattattct ccacgtgcca gtgatttcga atcgctgtcg caaacttctc
    12541 agcggttaaa tcagcagaac tgatataata tctgaccatc atttcgggct ctttcttttg
    12601 ctctgctatt attgagcgaa aggagactgc cacgcataat tttttcagcc ctttccattc
    12661 aaacgtgaaa tcaataagtt catcagggac atcgcaaaca atatgaagac ggatttcttc
    12721 tctgccgtga ctcttttcac tcattgcgga actgtcatgc gctggattat ttaattctgt
    12781 cagcggaaat tttttctcaa aggctttatt tagccgcccc tggtttcctt ttaccgcaaa
    12841 taaataatca cctccctgtt tttgtatctt ctctgcaata tctttctggc aacccatcgc
    12901 atcagttgtg atgatttttc ctttaatatc cagcatgtta agaagttctg ggatagctgt
    12961 aatctcatta gatttctcat ccgtcttgat ctgtccgatg accagactgt gcattgttga
    13021 gaacgcacta atgacatgaa tcgctcccct gcggcgactc ttgtcataag agtgccggag
    13081 cgtttttcca tcaattgcaa tgacgtcttt atcatctgta gaatggcagt cacgcatcca
    13141 gttaataaag cactcgtgaa attttgcagg actgatacag gatacaactc tggcaatggt
    13201 atcgtgaaca ggaatgccat tttcaaaatc accatattgc ttcaaaaaat cgagatgtgt
    13261 ttccccaaaa tcctctatat cttcccaacc ctctgcacca gaaataacgg cacaaatagt
    13321 caacagtaga atatccgata atttatgttc cactttccag gcttgtctgt aatcggggat
    13381 aatagaaata tgttccatca attttttaag ttccattttg ttctccttaa ttatgtaaga
    13441 agtatttgat catgtataag caataaaaaa cagcttcagg taataaggaa tatctcaatt
    13501 tttaaacata aaatgcgaat tatttagtac aaaaagtgaa cttctgatga tctttccctg
    13561 gcctgccacc atctcttcac aagcttatgc ttattacatg ttacatgcaa aacgtcccgt
    13621 tttgcatctt tgtgcctacg gcacaccgtg atctttccct gatgagggat atattaacta
    13681 ttgaacatta gcgcgaatta tttagtgcaa aaaatcagca tcacgtgatg cttttcctgc
    13741 tattaataac ctcctctatt taactatatt tttcattgat gcaattcgta gatttgaaga
    13801 agttcaactg ccactttttt atttttacta tcgcaatttt tataccatac ccatgcttcc
    13861 gtatttttta ctatttttaa atcatccata ataaaatccc caccaaaaac agtaaaatac
    13921 aatgtttcct cattaaaaat aaaactcact gccccccgtt actttattcg taccccttat
    13981 aggtaatgac t
//
LOCUS       DAETQN010000114        13903 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.114, whole genome shotgun sequence.
ACCESSION   DAETQN010000114 DAETQN010000000
VERSION     DAETQN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13903
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.114"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            242..1495
                     /gene="kch"
                     /locus_tag="K0W68_003341"
     CDS             242..1495
                     /gene="kch"
                     /locus_tag="K0W68_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBI3074812.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1550..1723)
                     /locus_tag="K0W68_003342"
     CDS             complement(1550..1723)
                     /locus_tag="K0W68_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBI3074813.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1866..3326
                     /gene="cls"
                     /locus_tag="K0W68_003343"
     CDS             1866..3326
                     /gene="cls"
                     /locus_tag="K0W68_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBI3074814.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3361..3690
                     /locus_tag="K0W68_003344"
     CDS             3361..3690
                     /locus_tag="K0W68_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBI3074815.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="K0W68_003345"
     CDS             complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="K0W68_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBI3074816.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="K0W68_003346"
     CDS             complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="K0W68_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBI3074817.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="K0W68_003347"
     CDS             complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="K0W68_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBI3074818.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="K0W68_003348"
     CDS             complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="K0W68_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBI3074819.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="K0W68_003349"
     CDS             complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="K0W68_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBI3074820.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            9613..9909
                     /locus_tag="K0W68_003350"
     CDS             9613..9909
                     /locus_tag="K0W68_003350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074821.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="K0W68_003351"
     CDS             complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="K0W68_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBI3074822.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11192..13867
                     /gene="adhE"
                     /locus_tag="K0W68_003352"
                     /gene_synonym="adhC"
     CDS             11192..13867
                     /gene="adhE"
                     /locus_tag="K0W68_003352"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBI3074823.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3658 a         3437 c         3230 g         3578 t
ORIGIN      
        1 cttatagccg gtcagtgcat tcactaccac aagcagataa taactttatc tgtatttctt
       61 tatatgatgt gagcatattc aggaaaaatg acctttcctg aggtacagtt gctcgctcaa
      121 atcctttgat tactgcggat aaagtcatct actattgttt aacacattca cgcgctgcat
      181 gtatgttcag aaaatgtgca cgctaagata attaaacaaa attgtggtta cagaggtatc
      241 agtgagtcac tgggctacat tcaaacaaac agcaacaaat ttatgggtga cccttcggca
      301 cgacattctc gcgctggccg tctttttaaa tggattgctt atttttaaaa caatctatgg
      361 tatgtcggtc aatttgcttg atattttcca tatcaaagca ttttcagagt tggatctctc
      421 cttgctggca aacgccccac tatttatgct cggcgtcttt cttgtcctga actccattgg
      481 cttactgttc cgggcaaagc tcgcatgggc aatcagtatc attttgttgt tgatagcgct
      541 aatttacacc ctgcattttt atccctggct gaaatttagt attggatttt gcatttttac
      601 gctggtgttt ttgctgatac tgcgcaaaga cttctcccac agtagcgccg cagccgggac
      661 aatttttgca tttattagtt tcacgacatt actgttttac tcaacctacg gtgcgcttta
      721 tttaagcgaa ggttttaatc cgcgaataga aagtttgatg accgcgttct atttttcgat
      781 agaaaccatg tcaaccgtcg gctatggcga tattgtccct gtttctgaat cagcacgatt
      841 gttcactatt tcggtcatta tttccggcat taccgttttt gccacatcta tgacctcaat
      901 ttttggtccg cttatccgcg ggggattcaa caaacttgta aaaggaaaca atcatacaat
      961 gcatcgtaaa gatcatttta ttgtttgcgg acattcgatt ctcgccatca atacgatcct
     1021 gcaactgaat caacgcggac aaaacgtaac ggttatcagc aacttgcctg aagatgatat
     1081 caagcaactt gagcaacgct taggcgataa cgctgatgtt atccccggtg acagtaatga
     1141 cagttcagta ttaaagaaag cgggaatcga tcgttgccgg gccattctgg cgctgagtga
     1201 taacgatgca gataacgcgt ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa
     1261 aacagttctc gccgtcagtg atagcaaaaa cctgaataag attaagatgg tacatccgga
     1321 tatcattctc tcaccgcaac tgtttggcag cgaaattctg gcgcgagttt taaatggtga
     1381 agagattaat aatgatatgc tcgtttcaat gttgttgaac tccggtcatg gcattttcag
     1441 cgataacgat gaacaagaaa cgaaagctga cagtaaagaa tcagtgcaaa aatagagttt
     1501 aaagacgaaa gagtaaactt attgggcacc gcaatggtgc ccagtatatt tagatattgt
     1561 agatcgcaaa caataacgag ttacgctgtt tgtttagaat gcacttcctg atgctgtgga
     1621 tacgcatatt tcggcgcgat tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac
     1681 gctgcatgcg ccagcgcccc acttccgttc tactccgctt catgttaact actctatgca
     1741 ataacagaat ggtcattata cccccacccc cgacgctgac cagtgctttt cccgcgtttt
     1801 tatttgccag atgaatccat atgcgtaaac tcataacaat gcgctttcaa aaggatttct
     1861 aatctatgac aaccgtttat acgttggtga gttggttggc cattctggga tactggttgc
     1921 tcattgcagg cgtaacttta cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg
     1981 cctggctgtt gattatttac attctgccgt tagtcggaat tattgcctat cttgccgttg
     2041 gcgagctcca tttaggcaaa cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg
     2101 caaaatggct taacgacctt aaagcctgta agcatatctt cgccgaagaa aatagcagtg
     2161 tcgctgcgcc attattcaag ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga
     2221 atcagctaca actgatgacc gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc
     2281 agctcgcgcg ccataatatt gagatggtgt tttatatctg gcagcccggc ggcatggcgg
     2341 accaggtggc tgaatcatta atggcggctg cacggcgtgg tattcattgc cgattgatgc
     2401 tcgactccgc cgggagtgtg gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg
     2461 ccggtattga agtggtcgaa gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta
     2521 tggacctgcg ccaacatcgc aagatgatca tgatcgataa ttacatcgcg tacaccggca
     2581 gcatgaatat ggtcgatcct cgctacttca aacaagatgc gggcgtaggg caatggattg
     2641 atctgatggc gcgtatggaa ggccccatcg ccaccgcgat ggggattatt tattcctgcg
     2701 actgggagat tgaaaccgga aaacgtattc tgccgccacc accagatgtc aatattatgc
     2761 cgtttgaaca ggccagcggt cacaccattc acacaattgc ttctggcccc ggctttccgg
     2821 aagatctcat tcaccaggca ttattgactg cggcttattc ggcgcgtgaa tatttgatca
     2881 tgacaacgcc ctactttgtg ccaagcgatg atttacttca tgcgatttgc acggcggcgc
     2941 agcgcggggt ggatgtcagt attatccttc cgcgaaaaaa tgactcgatg ctggtcggct
     3001 gggccagtcg cgcattcttt acggaactgc ttgctgctgg ggttaaaatt tatcagtttg
     3061 aaggcgggtt actgcatacc aagagcgtgc tggtcgatgg cgaactaagt ctggttggca
     3121 ccgttaacct tgatatgcgt agtctgtggc tgaatttcga gattaccctg gcaatcgacg
     3181 ataaaggttt tggtgcagac cttgccgccg ttcaggacga ttatatttcg cgttcacgtc
     3241 tgctcgatgc ccgtttatgg ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt
     3301 tttacttctt cagcccgttg ctgtaaaacg tcgccaacag aggttaaaca ggtagtgact
     3361 atggatatgg atctaaacaa tcgcctgact gaagatgaaa cgcttgagca ggcctacgat
     3421 atttttctcg aactggctgc cgacaatctc gacccagctg atgttctgct gttcaatctt
     3481 cagtttgaag agcgcggcgg cgcggagtta ttcgatccgg cggaagactg gcaggaacat
     3541 gttgatttcg atttgaaccc ggactttttt gccgaagtgg tgattggtct ggcggacagt
     3601 gaggatggtg aaatcaacga tattttcgcc cgcattctgt tatgccgcga aaaagatcac
     3661 aaactttgcc atatcatctg gcgggaataa ataatcgctt tcggcgtgaa aaagggctga
     3721 caactgtcag cccttattgt gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc
     3781 tgaaactccc ttccagcaca ggacgtgttt tggcgcactc cggaccggca atcgggcaac
     3841 aggtacggaa aacacaaccg gaaggcgggt tgatcggcga cggtaattcc ccttccagta
     3901 actggatggt tttgttcttc tccagatccg gatcaggtat ggggactgcc gacatcaatg
     3961 ccctggtgta aggatgtagt ggattgtggt agacctcatc ataggtcccc agttctaccg
     4021 catggccgag atacatcacc aacacacgat cggaaatgtg ttttaccacg gccaggtcat
     4081 gagcgataaa aattaatgac aatcccatct cacgttgcag ctgctggagc aggttgacca
     4141 cctgcgcctg aattgacacg tccagcgccg aaatcggctc atcgcagata atcagcttcg
     4201 gttcaagaat aagagcacga gcaatcccga tacgctggca ctgcccaccg gagaactcat
     4261 gcggatagcg gttaatcagg ttaggcaata acccgacttt cagcatcatc gccttcacgc
     4321 gctcgcgaac ttcctggcgt gacattttcg gatgataagt acgcagtggt tcagcgatga
     4381 tctcgccgat ggtcatacgc gggtttagcg atgccaacgg atcctggaaa atcatctgaa
     4441 tatcactgcg aacggcacgc cattcatcgg gcttcatgcc cagcaactct ttacctaacc
     4501 aggcaacatg accgccggtc gctttaacca ggccaatgat ggcgcgagca aaggtggact
     4561 taccgcatcc cgattcccct accacaccta atgtttcccc ttcatacagg cgaagcgtga
     4621 caccatcgac ggctttgaga gttttcggcg gttgccagaa ccactgtttg ccatctttga
     4681 tttcaaagtg cactttcaga tcggcgattt cgaggaggac ttttcttcct tcggttacag
     4741 cattcataac agttcctcca ccggtttaaa gcaagcacgc agacggccag gcgtaaactc
     4801 ttccagcggc ggtgcgctac tacaaatttc catcgcatgc ggacaacgtg gctggaacgg
     4861 gcaacctttc ggtaatcgca gcaggtttgg cggattacca gggatggtca acattgtttc
     4921 accttccgca tcgagacgcg gcaccgcgtt gagcaaaccg atagaataag gatgaacggg
     4981 ttgataaaag acatcgcgcg cgttgccata ttccatcgtg cgcccggcgt acattaccag
     5041 cactttgtcg cagatccccg ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt
     5101 attaaattcc cgcttcagtt catttaatag cgtcatgatc tgcgcctgta cggtgacgtc
     5161 cagcgcagta gtgggttcat ccgcaatcag cagcttaggt cgacatagca atgccatcgc
     5221 aatcataact cgctgacgca tgccaccaga aaattcgtgc gggtacattt tcatgcgttt
     5281 acgtgcttcc ggcattttta ccgcatcgag catccgcacc gactcttcaa acgcctcagc
     5341 tttgctcatg ttcttatgca gcatcagcac ttccatcaac tgctcaccga cgcgcatata
     5401 gggattcaac gaagtcattg ggtcctgaaa aatcattgag atttgttcag cgcgcagttt
     5461 attgagttca cgctctggca aattgaggat ttcacgccca ttgaaggttg ccgatccgcc
     5521 aatacgtccg ttggcagcca acaggcccat caacgcaaat gcagtttgcg atttacccga
     5581 accagactca cctacaatgc ccagcgtttc tccggcacgt agagaaaaat tcaaatcatt
     5641 gaccgccgtg acatcgccgt ccggggtact aaaggtgaca cgcaaatctt tcacgttcag
     5701 cagtacgtca gcctgttgtt gtgcgagcgg cacagttgca gtttcaatta cgctcatggc
     5761 tgcactcctt aacgatcttt cgggtcgaga gcatcacgca agccatcgcc gataaagttg
     5821 aaacaaaaca gcgtcaccac gaggaatccc gctgggaaca acaataacca tggagagact
     5881 tccatcgagt tcgcgccatc actcagcaat gcaccccagc tgcttaacgg ctcttgcgta
     5941 cccaacccca ggaagctaag gaaagattca aagaggatca tgctgggcac cagaagtgat
     6001 gcgtagacca ccaccacacc gagtacattc ggcacaatgt ggcgaataac aatgcccgat
     6061 gtcgatacac cgccaacttg tgccgcctca ataaactctt tgcgcttcag actcagggtt
     6121 tgtccacgca caatacgagc catatccagc caggaaacca tgccaatcgc cacgaaaatc
     6181 agcaggatgt tttgaccgaa aaaggtcaca agcaaaatga cgaagaacat gaatgggaag
     6241 gagttgagga tttccagcag acgcatcatt accgaatcca ctttaccgcc cagataaccg
     6301 gaaagcgaac cgtaaagtgt ccccacgacc actgccacca gtgccgcagc aacaccgacc
     6361 atgagtgaga tacgcccgcc aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca
     6421 gtaccaaagt agtgaccgga ctccatatcc ggggcgctgg acatcatcgc ccagtcagta
     6481 tcgtcatagg caaactgcga aagcatcggt gccaggatta caaataacgc gatcagcacc
     6541 agcactatca gactggcaac cgccgcacgg ttatgcataa aacgtcgacg tgcgtcctgc
     6601 cacaagctgc gcccttcgac ctccagcttt tcactgaaat tttccagcgt ctcgctgttt
     6661 ttcttactta acatcatcgc gagctccagt atcagtaacg gattttcggg tcgataaccg
     6721 catatagcac atcgacaatg gcattaaaca aaatggttaa agcaccaacc aggatggtca
     6781 ggcttaacac taaggaatag tcacggttca atgcgccatt aacgaacagt tgtccaatcc
     6841 ccggcaaacc ataaatggtt tcgataacca tagaaccggt aataatgccg acaaatgcgg
     6901 ggcccatata agagagcacg ggtaacagag caggttttaa tgcgtggcgt aaaatgatcc
     6961 gccgcatagg taaccctttc gcccgggcag tacgaataaa gttggagtgt aatacttcaa
     7021 tcatagagcc tcgggtaata cgcgcaatac tggcgatata agcgagtgac aacgccacca
     7081 tcggcaatat catgaattta agcgccccac cattccagcc accgcccggc agccaatgca
     7141 aaatgatcgc aaatatcatg actaataatg gcgcaaccac aaaactgggg ataacaaccc
     7201 cggtcattgc cagccccatc acggtatagt cccatttggt gttttgtttt aatgcgacaa
     7261 taacgccagc actaacaccc agtattaccg caaggaaaaa tgcggcggct cccagtttgg
     7321 cagaaacggg aaaactggat gcaaccaggt cattgaccga ataatcttta tatttaaacg
     7381 atggaccgaa atcaccgtgc gccagttgtt tcaggtagct gaaatactgt gtcatgatcg
     7441 gatcattaag atgatatttc gcttcgatat tggccatcac ttccggcggt aaagtacgtt
     7501 cgccggtaaa agggcttccc ggcgcgaggc gcatcataaa gaacgaaata gtaataagaa
     7561 taaatagcgt cggaatcgct tccagacagc gacgtagaat aaattttaac attgccctac
     7621 cttctggcct gtgccttctg taatgcgata aaaatcagac accgtggagc aggacactcc
     7681 tgccccacgt attgccatta gtgcttcaca atgtacatat tccgggtgta ggtattatcc
     7741 agcggatctt tgccggtata gccaccaacc cacggtttca ccagacgcgc attcacgtag
     7801 taataaacag gaacaatggc cgaatcctta tccagctgtt gttctgcttt agtgtacaga
     7861 gctgtgcgct gcgcctcgtc agtcactttc agcgtttccg ccataatgct gtcaaaggcc
     7921 gggctcttat aatgcgcggt attcatcgag ctgttcgaca gcatggtgtt caggaaggaa
     7981 gttggttcgt tgtagtctgc acaccagcct gcacgggcca catcaaaagt gccctggtga
     8041 cgggtgtcga ggaacgtttt ccactcctgg ttaaccagtt tgacgtttac accaatgttt
     8101 ttcttccaca atgaagaggc agcaatcgcc agctttttat gcagatcaga ggtgttatac
     8161 agcaggttga tggtcaacgg tttgtctgcg gtataacccg cttcagccag cagttttttc
     8221 gcttcttcgt tacgtttttc ctggctccag ccaaaccatt ctggctgagt caattttgcg
     8281 ccatcagtat acggtggagt gtaaccatag gcgggcatgt cgccctgcgc tttcacttta
     8341 ttaacaatga tatcgcggtc catacctagt ttcagtgcgg tacgcacacg cacatcgttg
     8401 aatggcggtt tctggttgtt aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga
     8461 acttcgtccg ggatctcttt tttcagcttc tggaacaatt cgatcggcat gttgttatac
     8521 gtcatgtcga tttcaccact acggtagcgg ttgacatcgg taacttcaga agcaataggc
     8581 aaataggtta cctggttaat aacggttttc gcgttgttcc agtaggtcgg gctgcgttca
     8641 agaacgattc gttcgtttac gacccaatct tttaaggtat aggcaccgtt ggtgacgata
     8701 ttaccaggct gggtccattt ttcgccgaat ttctcgatag cggcttttgg caccggtgaa
     8761 gttgatgggt gaacaagtaa tttatagaag tacggaacgg gttcacttaa ggtgacttct
     8821 aatgtgtgat catcaatagc tttcacgccg agatcggtaa tcggtttttt cccttcaaga
     8881 atttcatcaa taccggcgat atgcccatat tgcagataac tggcatacgg agaagcagtg
     8941 ttcggatcaa cagaacgttg ccagctatac acaaagtctt gtgctgtgac tggcgtgcta
     9001 tcagaccatt tcgcatcttt acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc
     9061 caggattcag cgacgccagg tgctggatga ccgtcaagat cgctgaccag taagccttca
     9121 aacaggtctc ggctgatatt agactccgga acaccttcaa ttttgtgcgg atctaatgac
     9181 tgaacttctg aaccattgtt acgtaccagt gtttgttttt ccgccagtgt gacgcctgcg
     9241 ggtacatcag ctgccagcgc gacattccct gccattagcg cagccagaac gccagctgct
     9301 actaaacttc tcttggtgat gttggtcatt gttttttgga ctccctcatt ataattactg
     9361 gtattaacca gcatgtgtaa tcccggattg ggaccctgca caggctcgga gactttctgc
     9421 tgtcaggtta ctttatcgtt acgtattact atcaccgact ttatttattc tggtgattca
     9481 gacgatcacc ttatgtcgat tcgtactgtg tctcccctgt cgagacacat tattctttcg
     9541 cgacgctctt taattgttaa ttattctcat taccaataaa gaatcgtctg gcggttgccc
     9601 ggaaagtacc aaatgcggtt cttactcgcc aatacatttt gcaaaattgt taagcaattc
     9661 tcttttattg accgtatcag aatctatagc ttgcatccat gccttaacaa acatctttca
     9721 attcaatcaa atagagattg gctaagcatg tctatctttt ctgattacag ttccagtagt
     9781 gaaatgcaca ataatttaac aattgattat tatttagcac tttcctctac caaaggcagt
     9841 ggaattacta atatcatttc aattattctt cagcaagccc aggattatga tgtggcgaaa
     9901 ataacatgat ggtgaggaga agcagttaag gaaatcctta taactaactg taatttgaga
     9961 agaatatttt tacctattcc ttttgctttt gataactgcg cgggaacaaa gtaaaaataa
    10021 tgacgaagta aacggagcat ggcagctccg tttcatttga aaggaattaa ttaagcaggc
    10081 cggggaaaat ccccttaata ccagtaacga taaattcaat ccccaatgcc atcagcaata
    10141 gccccataat acgcgtaatc acgttgatgc cggtctggcg taaaacccgt accagccacg
    10201 gtgccatgcg gaacaatccc cagcaacata aagcgaacaa tgcaatagcc acaaagaaac
    10261 caaacagata gctaatgctg tgataacgcg taccccagac gatggtagaa ctgatcgccc
    10321 ctggccccgc catcaacggc aacgccaatg gcaccacacc aatgctttca cgtaccgcgg
    10381 tttctgattt ttcttgcttg ttctgtttat cctcgccaag cttgccgctg atcatcgaca
    10441 tcgctatcgt caccaccagg atgccccctg cgatacggaa cgaatcaatt gatataccaa
    10501 aaagttgcag aatcgtgtcg ccgagaaaaa gcgagatcca caagataatg gccacagaca
    10561 ggttggctgt aaggttagtt ttgtttcgcg ctgccgctgt ctgataactg gtcatgctga
    10621 taaagacggg aataatccct accgggttga ccagcgcaaa taacccgatg aaaaatttga
    10681 agtaaacggg aaaatcaaaa aaggtctgaa tcacggttag ctccgaagca aaagccggat
    10741 aatgttagcc ataaattaag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
    10801 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
    10861 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
    10921 aagcgatgct gaaaggtgtc agcttagcaa aaatttgatt tggatcacgt aatcagtacc
    10981 cagaagtgag taatcttgct tacgccacct agaagtgacg cattagagat aataactcta
    11041 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
    11101 ataggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
    11161 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
    11221 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
    11281 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
    11341 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
    11401 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
    11461 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
    11521 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gtctgaagac
    11581 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgccacca acaaagcggc
    11641 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
    11701 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
    11761 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
    11821 cggtgtaggc gcgggcaaca ctccagttgt tatcgatgaa actgctgata tcaaacgtgc
    11881 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
    11941 gtctgttgtt gttgttgact ctgtttatga cgctgtacgt gaacgttttg caacccacgg
    12001 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaacgg
    12061 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
    12121 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
    12181 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
    12241 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
    12301 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
    12361 aacggctcgt atcctgatta acaccccagc gtctcagggt ggtatcggtg acctgtataa
    12421 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tgggggggta actccatctc
    12481 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
    12541 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
    12601 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
    12661 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
    12721 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
    12781 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
    12841 cgccgcgaag attatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
    12901 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
    12961 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
    13021 tgtaactgac gacgctactg gtcagaaata tccgctggca gactatgcgc tgactccgga
    13081 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
    13141 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
    13201 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
    13261 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
    13321 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
    13381 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
    13441 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
    13501 ggctcgccgt cgttatgctg aaattgccga ccacttgggt ctgagcgcac cgggcgaccg
    13561 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
    13621 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
    13681 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
    13741 gatctccgag ctgaaacaga tcctgctgga tacctactac ggtcgtgatt atgtagaagg
    13801 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
    13861 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttc
//
LOCUS       DAETQN010000115        13807 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.115, whole genome shotgun sequence.
ACCESSION   DAETQN010000115 DAETQN010000000
VERSION     DAETQN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.115"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(2..760)
                     /gene="dsbG"
                     /locus_tag="K0W68_003353"
     CDS             complement(2..760)
                     /gene="dsbG"
                     /locus_tag="K0W68_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBI3074824.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGVMTPTY"
     gene            1132..1695
                     /gene="ahpC"
                     /locus_tag="K0W68_003354"
     CDS             1132..1695
                     /gene="ahpC"
                     /locus_tag="K0W68_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBI3074825.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1866..3431
                     /gene="ahpF"
                     /locus_tag="K0W68_003355"
     CDS             1866..3431
                     /gene="ahpF"
                     /locus_tag="K0W68_003355"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBI3074826.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3552..3980)
                     /gene="uspG"
                     /locus_tag="K0W68_003356"
     CDS             complement(3552..3980)
                     /gene="uspG"
                     /locus_tag="K0W68_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBI3074827.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4201..5439
                     /locus_tag="K0W68_003357"
                     /pseudo
     CDS             4201..5439
                     /locus_tag="K0W68_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            complement(5670..6080)
                     /gene="rnk"
                     /locus_tag="K0W68_003358"
     CDS             complement(5670..6080)
                     /gene="rnk"
                     /locus_tag="K0W68_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBI3074828.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6310..7116)
                     /gene="rna"
                     /locus_tag="K0W68_003359"
     CDS             complement(6310..7116)
                     /gene="rna"
                     /locus_tag="K0W68_003359"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBI3074829.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7230..8693)
                     /gene="citT"
                     /locus_tag="K0W68_003360"
     CDS             complement(7230..8693)
                     /gene="citT"
                     /locus_tag="K0W68_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HBI3074830.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8744..9622)
                     /gene="citG"
                     /locus_tag="K0W68_003361"
     CDS             complement(8744..9622)
                     /gene="citG"
                     /locus_tag="K0W68_003361"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBI3074831.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9597..10148)
                     /gene="citX"
                     /locus_tag="K0W68_003362"
     CDS             complement(9597..10148)
                     /gene="citX"
                     /locus_tag="K0W68_003362"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBI3074832.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10152..11683)
                     /gene="citF"
                     /locus_tag="K0W68_003363"
                     /pseudo
     CDS             complement(10152..11683)
                     /gene="citF"
                     /locus_tag="K0W68_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
     gene            complement(11694..12602)
                     /gene="citE"
                     /locus_tag="K0W68_003364"
     CDS             complement(11694..12602)
                     /gene="citE"
                     /locus_tag="K0W68_003364"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBI3074833.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12599..12895)
                     /gene="citD"
                     /locus_tag="K0W68_003365"
     CDS             complement(12599..12895)
                     /gene="citD"
                     /locus_tag="K0W68_003365"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBI3074834.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12910..>13807)
                     /gene="citC"
                     /locus_tag="K0W68_003366"
     CDS             complement(12910..>13807)
                     /gene="citC"
                     /locus_tag="K0W68_003366"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBI3074835.1"
                     /translation="AGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTK
                     TEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANP
                     FTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEY
                     IISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYN
                     QDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTL
                     HYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3390 a         3497 c         3673 g         3247 t
ORIGIN      
        1 atcaataagt tggagtcatt acccccataa tgatattaag cgttttctga tcgggcaacc
       61 ccacggcctg ttgcagcgta ttttccttac tcatgtaata gatagccggc gtgacatttg
      121 cccccagatc gtccatcagt ttctcattgt cacttaacac tttcatttgc tctgtactta
      181 cgtttgcagg cacgtttagc ttaagcttgc caccagaggc ttcatattgt tgccaggttt
      241 ttgcgggatc tttggaggca agaattgccg ctgctgtcgc cgggctttct ggcttgataa
      301 ccccaaccaa caatgttctt aattgcactt tgccagaatc tacccacggg cgcgcctgct
      361 gccagaactg tttacaatat gggcagaacg gatcggcgaa gacgtagaca atgaccggcg
      421 catctttttt accgtcgagg atccagtggg attgttccat ccgttgccac atttcgcgtc
      481 cggctggtgc gtaaatttct ttttcgataa gtgtgttact caggttttca cctttctcgt
      541 tgtacatgta accagagata gcgtgcttac catcgggagt caggtagatg gtgacgccca
      601 tatcctgata ctttccgaga taacctttca ttcctccggg ggcatcgaac gttttgatga
      661 ttgtaatgcc ctgtttttca atcgctttta ctggggaagg aagttcctct gcgaaggcga
      721 ttgcaggaag cagagccagt aaaagtatct tttttaacat taatttgtcc ttttcagtca
      781 gtgcaaaagt cgagtaaaag gcataaccta tcactgtcat aggtaagagc ttagatcagg
      841 tgattgccct ttgtttatga gggtgttgta atccatgtcg ttgttgcatt tgtaagggca
      901 acacctcagc ctgcaggcag gcgctgaaga taccaaaggg tagttcagat tacacggtca
      961 cctggaaagg gggccatttt actttttatc gccgctggcg gtgcaaagtt cacaaagttg
     1021 tcttacgaag gttgtaaggt aaaacttatc gatttgataa tggaaacgca ttagccgaat
     1081 cggcaaaaat tggttacctt acatctcatc gaaaacacgg aggaagtata gatgtccttg
     1141 attaacacca aaattaaacc ttttaaaaac caggcattca aaaacggcga attcatcgaa
     1201 atcaccgaaa aagataccga aggccgctgg agcgtcttct tcttctaccc ggctgacttt
     1261 actttcgtat gcccgaccga actgggtgac gttgctgacc actacgaaga actgcagaaa
     1321 ctgggcgtag acgtatacgc agtatctacc gatactcact tcacccacaa agcatggcac
     1381 agcagctctg aaaccatcgc taaaatcaaa tatgcgatga tcggcgaccc gactggcgcc
     1441 ctgacccgta acttcgacaa catgcgtgaa gatgaaggtc tggctgaccg tgcgaccttc
     1501 gttgttgacc cgcagggtat catccaggca atcgaagtta ccgctgaagg cattggccgt
     1561 gacgcgtctg atctgctgcg taaaatcaaa gcagctcagt acgtagcttc tcacccaggt
     1621 gaagtttgcc cggctaaatg gaaagaaggt gaagcaactc tggctccgtc tctggacctg
     1681 gttggtaaaa tctaaatttc cttcgtcttt cgcgctacca gctccggcgc gaaatacttc
     1741 ggaaattcac ctaattcttc gggtgctgcg gcacccgatt ttcttccccg caccatgatg
     1801 caagctgcat ccaggtagcc gtagaggccg cttgcatgat gatgtttaaa gcccaggaga
     1861 taaacatgct cgacacaaat atgaaaactc aactcaaggc ttaccttgag aaattgacca
     1921 agcctgttga gttaattgcc acgctggatg acagcgctaa atcggcagaa atcaaggaac
     1981 tgttggctga aatcgcagaa ctgtcagaca aagtcacctt taaagaagat aacagcttgc
     2041 cggtgcgtaa gccgtctttc ctgatcacca acccaggttc caaccagggg ccacgttttg
     2101 caggctcccc gctgggccac gagttcacct cgctggtact ggcgttgctg tggaccggtg
     2161 gtcatccgtc gaaagaagcg cagtctctgc tggagcagat tcgccatatt gacggtgatt
     2221 ttgaattcga aacctattac tcgctctctt gccataactg cccggacgtg gtgcaggcgc
     2281 tgaacctgat gagcgtactg aacccgcgca tcaagcacac tgcaattgac ggcggcacct
     2341 tccagaacga aatcaccgat cgcaacgtga tgggcgttcc ggcagtgttc gtaaacggga
     2401 aagagtttgg tcagggccgc atgacgttga ctgaaatcgt tgccaaaatt gatacgggcg
     2461 cggaaaaacg tgcggcagaa gagctgaaca agcgtgatgc ttatgacgta ttaatcgtcg
     2521 gttccggccc ggcgggtgca gcggcagcaa tttactccgc acgtaaaggc atccgtaccg
     2581 gtctgatggg cgaacgtttt ggtggtcaga tcctcgatac cgttgatatc gaaaactaca
     2641 tttctgtacc gaagactgaa gggcagaagc tggcaggcgc actgaaagtt cacgttgatg
     2701 aatacgacgt tgatgtgatc gacagccaga gcgccagcaa actgatccca gcagcagttg
     2761 aaggtggtct gcatcagatt gaaacagctt ctggcgcggt actgaaagca cgcagcatta
     2821 tcgtggcgac cggtgcaaaa tggcgcaaca tgaacgttcc gggcgaagat cagtatcgca
     2881 ccaaaggcgt gacctactgc ccgcactgcg acggcccgct gtttaaaggt aaacgcgtag
     2941 cggttatcgg cggcggtaac tccggcgtgg aagcggcaat tgacctggcg ggtatcgttg
     3001 agcacgtaac gctgctggaa tttgcgccag aaatgaaagc cgaccaggtt ctgcaggaca
     3061 aactgcgcag cctgaaaaac gtcgacatta ttctgaatgc gcaaaccacg gaagtgaaag
     3121 gcgacggcag caaagtcgtt ggtctggaat atcgagatcg tgtcagcggc gatattcaca
     3181 acatcgaact ggccggtatt ttcgtgcaga ttggtctgct gccgaacacc aactggctcg
     3241 aaggcgcagt cgaacgtaac cgcatgggtg agattatcat tgatgcgaaa tgcgaaacca
     3301 acgtcaaagg cgtattcgca gcgggtgact gtacgacggt tccgtacaag cagatcatca
     3361 tcgccactgg cgaaggtgcc aaagcctctt tgagtgcttt tgactacctg attcgcacca
     3421 aaactgcata agaagaagta agattcacct gcaattgctt agccgccggg gtcaaacctg
     3481 gcggcttttt tatggcatta aaaagccctg ccgggatgtt ccaggcaggg cggcataaat
     3541 acttttatct gttagcgaat gaccagtaca ggcagattgg cgtggcggat cacgctcgag
     3601 gcgttagaac ctaacagatg ggtcgaaatc gatgggttgc gagaaccgat aactacaaca
     3661 tcagccccca gttcttctgc caactcattg acttcatccc gcacgctacc aaaacggaca
     3721 tgttgtttaa tgcgggaagg atcgatggtg aagtggctga ccatcgtttg cagacgttct
     3781 tctgcttcat gttgcagatg ctcttcaaaa cgacgcacat cagcggcaaa acggtgcagg
     3841 ctcaggctgg ctgacccggg tagtacgtga agtagatgaa taactccgtc atcctgggcg
     3901 aggaattcag cgtggcgaac agctttgtcg ctcaattcca tttcaaatac atcaactggc
     3961 ataatgattg tcttatacat aaccctttct ccctgttaat catgaacaaa tcattcgcca
     4021 tgattataat atttatccct gatatttgtc tggttctttt tccttacgaa cggtttctgt
     4081 gattaatata ttctcactga acaccaggaa ttctcccaaa acctgcggta ccgcccgttt
     4141 tcccgctgtg atagctaccc ttaaagactg actctttttt gaactgtctc tggaggttgc
     4201 atgaaagcat tgacttatca cggcccacat cacgttcagg tagaaaatgt tcccgatccg
     4261 ggcattgaac aggctgatga tattattctg cgtattacgg caacggcgat ctgtggctct
     4321 gacctccatc tttatcgagg caaaataccc taggttaaac atggcgatat tttgggtcat
     4381 gaatttatgg gggaagtcgt tgaaaccgga aaggacgtaa aaaatttgca aaaaggcgac
     4441 cgggtggtaa ttccgtttgt cattgcttgt ggcgactgtt ttttctgtcg aatgcagcaa
     4501 tatgccgcct gcgaaaatac caatgcgggt aaaggcgctg cgctcaataa aaaacagata
     4561 ccagctcccg cggcattgtt tggttatagt cacctgtatg gtggcgttcc tggtggacag
     4621 gcggaatatg tccgcgtccc taaagggaat gtggggccgt ttaaagtacc gcctttgctt
     4681 tcagatgata aagccctttt cctttctgat attctgccaa cggcatggca ggcagcaaaa
     4741 aatgcgcaga tccaacaagg ttcaagcgtt gcagtctatg gtgccggtcc agtgggattg
     4801 ttgacaatcg cctgtgcacg gttgctcggt gcggaacaga tttttgttgt tgatcatcat
     4861 ccctaccgct tgcgtttcgc cgctgaccgc tacggcgcga tcccgattaa tttcgatgaa
     4921 gatagcgatc cggcacagtc aattattgaa caaacggcag gtcaccgggg cgtggatgca
     4981 gtaatagacg ccgtcggttt tgaagcgaaa ggcagcacca cggaaacggt gctgactaac
     5041 ctgaaactgg agggcagcag cggtaaagcg ttgcgccagt gtattgcggc ggtcaggcgt
     5101 ggcggcattg ttagcgtacc gggcgtctac gctggattta ttcacggttt cctgtttggc
     5161 gacgcctttg ataaagggtt gacgtttaaa atgggacaga cccacgttca cgcatggctg
     5221 ggtgaattat taccgttaat tgagaaagga ttactgaaac cagaagaaat tgttacccac
     5281 tatatgccgt ttgaagaggc cgcccgggga tatgagattt tcgaaaaacg tgaagaggag
     5341 tgccgtaagg tgattctggt acccggtgca caaagcgcag aggcggcgca gaaggcggtt
     5401 tcaggtctgg tgaatgcgat gccgggggga acaatatgat cgtcaggagt ggttttcgag
     5461 gtaaaggaca gccatgacga taatcgccgc cataatcaga aatcctatca ggatgtaaaa
     5521 tgcttctgcc atggttattc ccacaaacga aacggaataa ttttgcagca aaagtgaaca
     5581 gtgagaacca ggaaaaattg ctgattttgc gtaaagagga tgcgagtgca tcctctgggc
     5641 aaagcgagtt atcgcttgtg cagatgggat taaagcaggt agtcgccagc agcttctggc
     5701 tggtattcga gttccagcac ttcaaggtgg gttgcaacgc cgcccggaag ttcccagtga
     5761 atggaatcgc caacgcgcag ccccagcagt gcggcaccta ccggagccat aacggaaagc
     5821 tgagtattgc tatcggtcat ttttgccgga tacaccagcg tgcgcacacg cacttcgcca
     5881 tcgctaagat tgcggaattt aacccggctg ttcattgtca ccacgtcgtg tggcatctct
     5941 tctggcgaac acatttgggc gcgatccaac tctgcgttta acgcgtcggc gattggcaaa
     6001 ccagcatagg cgggttgctc cagcagaata tcgatgcgtt cggcatccag gtcgttaatg
     6061 atgatagttg gtctggacat ttttactcca tgtcgtcggt actgcgagtg tcgcagataa
     6121 acatacccaa aagaaaaccc tcaccgtcag gcggcgaggg tttaactcac atgatgatac
     6181 tgactgttgc tcactctttg aagtgatttg cgtcacattc agggaattcc tcaatgcagc
     6241 gcattatgta taattcttaa tcgtcacggt ttatggaaga cgaattagcg tgttttgtaa
     6301 atcagatgat taataaccca ctttatcaat cacaaagttt ttgccacagt tgcctgggtg
     6361 tggttgtggc aagaatgagt ttgcagaaag cggagcgttg atggcgtcag ctttgatcga
     6421 gatctgaatt tcagtcaaat acgcagggtt accctggcac gttagcttaa ctgctttcac
     6481 gttctctttt ccccagcttt tggcaaaggc ggcgtcaaag tcacggcggc tcactgtttt
     6541 accgtaatta tccgcaagga attttccggc ttcgctctct ttaatttctt gattcaggcg
     6601 taccatcgta ccgaagtatg cgtccggatc aaaaccaaag caggcaccgt gtttggcata
     6661 ttcgtagcgt tccaggcagg aacgtccgcc agctcctggc atgacttcac ttagtttagc
     6721 ggccgtttcc agtgataacc cggtttccgg cgatgaacac atccggctgg cgtgcgcttc
     6781 tggtagattc gggattgggc gagtagcgca accgaagcgc atccagcggc gttcatcaac
     6841 accacgggca gcaaccgatt taggcaatcc tggccacaga ccatgtacgg tcagaaaatc
     6901 agctttgttg gtcgtttcgg tttgtaggcg acattcatct cgttcgttac gatttcgatc
     6961 gtgttgactc tggcaaaatc cggtttgcca ggagagggcc aggacatagc gatcaaaatc
     7021 gccatactgt tttgcctgca acgctacggc gttggcagaa gagaagggaa gcagagaaac
     7081 cgcgagcaac gcggcgttac gccagaatgc tttcataatg ggtgtggaac tcatacatac
     7141 actgaatact atctattaaa tcataaaaaa gcccgccagg tgggcttacc tggcgggcgt
     7201 gatgatttat tcagcgtttg gcgaacgtat tagttccaca tggcgagaat cggccagcca
     7261 accaacagca acatagagat gtaaatcacc ccgaagattg cgccaagacg ccagtaatct
     7321 tttgatttca catagccaca gccgtaaata atcaccccag gaccggttgc atacggcgtc
     7381 agacagccca tgataccgat agacagcacc agcaggatac acagttgttc cattggtacg
     7441 cccggaatac ctttaccgac ggccagaata accgacagca tggttgcggt gtgcgcagac
     7501 aggctggcaa acaggtagtg tgcaaagtag aacaccagaa ccagtacaat caccgttgcg
     7561 tttggtgaga atccttccag gtgcgtactc atggtaccgg cgaaccagtc aataaagcca
     7621 gaacgagtca ggccgttagc catcacaacc agagttgcca ggttgaccag cgtgttccat
     7681 gcgctgttat agcgggtaat atctttccaa ggcacaacgt gcagggccag cattagcgaa
     7741 actgccagca gaccaactgc agtagcatta atgacttcac tgccaaaaac ccacaaacct
     7801 aagctgagca atacaaggcc aatcagtgtc cactctctgc gtgtcagcgc acccatggtt
     7861 ttcagttcat cacccgccca ggttgccact tcttcactgt gtgtgatttc cggtttgtac
     7921 agcacgtagg aaagccacgg cgcaatgata agcaagataa ccccaaccgg caggaagcag
     7981 aggaaccact gcaaccagct aatctggata ccggcaattt tgctgacgaa ctccagaccc
     8041 agcacgtttg gtgccgcacc ggtgacaaac atggacgaac tcagactggt actaatgacc
     8101 atcatccaca tcaaatagcc gccaatacga cgcgcggacg gatcgttggg gaatgattta
     8161 aacagcggcg gcaggttttt aatgaccgga aaaaccgtac ccccggtacg cgcggtgttg
     8221 gacggtgtaa acggtgccag cagaatgtcg ataatgacaa tcgcataacc caacgtcagc
     8281 gtgcgtttgc ccatgaattt caccaggaaa agggcaatgc gacgacctaa cccggaaact
     8341 tcatacccta atgcaaaaat aaatgcgcca aataccagcc ataccgtggt gctggaaaaa
     8401 ccagccagac cccatttcag cgcctgtttt tgcgcattaa acgctgggtc agctaattct
     8461 ttggcatcaa agagcaggta attactgcca ataacgcaaa tagtaaccgc aataaaactg
     8521 atcgctgttg ccggaattgg ctcgaggatc atgccgacaa tcattgccac aaacacagcg
     8581 aagtaatgcc atgcctgtgg tggcataccg tcggggacag ggataagaaa catgacaccc
     8641 atcaccacca gtggggccaa tagtttccat atattatctt ttgctaaaga catacgggtt
     8701 ctccgaaaat taatatttcc aaatttatca agtgcttaaa taattaaatc tgtgctaaaa
     8761 accaggtaag gatcagcagg tcggcactgc cgcctggact gagatttcgt tcgatacact
     8821 ccctgtcgaa ctgccggaga taatcgagat cggcgggggt tcgaatgccc cctttttgca
     8881 ataatgtttg cgcctcgcgc tgtagccagc gcaggccccc ctcgccaccg cgcgatgcaa
     8941 cgttggtatc gccgttgatc gccatcagta ggagcaaggt atcgagcaat gccagttcag
     9001 gatctaaccc ctgatccagc agagtgaggt aatgcggcaa ggcgtgattg atcaccagtg
     9061 gataacccgc ttcggcttca ccgcgtgcgc cggtaaggcc aagctgctgg tacaaccgtt
     9121 gacctgccgt cagttgtgaa ttattggtac gcagttctcg atcggtcagg ccacggcaga
     9181 aacttgccgc cgtagaacaa acggttgttg gcgttaccgg ttggttgagt tgaagcaaac
     9241 ggccaattgc cgcacatagt agccctaaag aaaaaatgct gcctttatgc gtgtttacgc
     9301 ccgcagtggc gcggaacata tcaccttcgc aagccatacc aattgggcgt aatccgtgga
     9361 gtaccgcttc tggtgctatt tccgcactac aggcaccaaa ttcaatgaaa cggggtagcc
     9421 agccctgaat cgccagcgcg ctgcggtgga aatcttccag cgccatatct ttatgtgcac
     9481 cgcagttaat ccgatccacg aggcctggtt tcggtgacag attgacttca gtcagcatgg
     9541 cgcgccagcc cagcagggcg tactcatcga tcaatgacgt cgcaagcttt gtggttttag
     9601 ttgacgttgc aggcatcgac atcgttcagc agtgcctcca tgcggttgag taaatcggtc
     9661 agttgatggg tttttccacg cgcgcagacg gctgcgcttt gctcgcacaa caggcagcgg
     9721 cgaggcggca gtgaatagtc gcggcgggag agaatttcgc cttcgggcgt caggacatcg
     9781 atatcccata accgcccgag aggatgacta tgttcaagct caatggtggc gagcttgagg
     9841 tcgcgagccg gggcggcaat gctcaacatg ccctccggcc cgctggcgga aaccagtgca
     9901 gcctgctcct gaatttgcca gccctgtttt gcggctaagg cacgcaaggc tgtcacgcca
     9961 tgattaaaaa ttcggcgtgt gacctcgctg tctttaatcg gcccaggcgc aaccacggta
    10021 aaggagacca gtggaacagg atggcgcttg agccagacgt gttgccgtgc ttgcctttca
    10081 tcccggctga cgagcagctc gggaattgac accgcatggt ggctggcgag ttcaggaagc
    10141 aggtgcatgg cttattcctt cacctgatgc acaacatcga tcaccgagcc atcgcggtaa
    10201 cgcacaacgg caacgacgcg gtcggtgaat tcaatcggct gtggttcacc ggtcagcaga
    10261 cgcgcgcgtt cgcgcagcca ctcaatggaa accactttaa tgcccgcttc ctgcagacgt
    10321 tctgccagtt ccggacgtgc cgggttaact gcgataccgt ggtctgtgac cagaatatcg
    10381 acactggagc ctggggtgat gcaggtcagc acgttatcca ccagagtcgg aatacgaccg
    10441 cgtaccagcg gcgcgacgat gatggaaagc gcagaggcaa tcgcggtatc cagtgaccac
    10501 cggaagcacc acgcagtacg ccgtcagagc cggtcagcac gttaacgttg aactgggtgt
    10561 caatttccag cgcgctcagt accaccacgt cgagacgatc aaccgatgcg cctttcgaac
    10621 cccagttagc gtactggttg gcgctgattt cgatgtgatt ggggttacgg gccagcgatt
    10681 gcgcagcatg gctgtcaaag ctctgcacat ccagcagttt gcggatcaga cctttttcgt
    10741 gcagatcaac catcgtcgcg gtaataccgc cgagggcgaa gtcggcgcga atatcgcggc
    10801 tacgcatttt gtcttccagg aaacgggtta ccgccagcga tgcgccgccg gtgccggttt
    10861 gcatggagaa accttctttg aagtagccag agttgacaat cacatccgca gcgctacggg
    10921 caataagcag ttcgcgcggg ttagtggtca tacgggtcgc gccagcgccg atttttgcag
    10981 catcgccaac gcggtcaact ttgacgatca aatcaacctg atcttgctca atgcttgccg
    11041 gattatgcgg ataaggcagc agttcttcgg taagcatcac aacctgtttt gcgttgtcgg
    11101 catcaaccat tgcatagccg agggagccgc agcaggcttt accggtgtaa ccgttggcat
    11161 taccgaattc atcacaggac gggacgccga ggaaagccac gtcgatattc agttcgccgc
    11221 tctgtaccag atgcacacgt ccgccgtgag agtggatttg caccggttcc gcaagcagac
    11281 cacgggaaat ttcttctgcc agtggaccgc gcaggccgga ggtataaatg cgggtaacca
    11341 cgccctggcg aatgtgttct accagtggcg catggcaatc actcagggag ctggacgcca
    11401 gggtcaggtt tttaaagccc atcttagcga tgacgtccat caccatattg acggtcaggt
    11461 caccgccacg gaaagcgtga tggaaggaaa ccgtcatgcc gtcctgtaaa ccagagcgac
    11521 gaatcgcttc ttcctggttg gcgcacagtt tgcgatcgcg cgctttttca gcctggtagg
    11581 tttgctttgg tgagttctgg aaagcggcaa gatcgcattc agcgcgacga ttccaggccg
    11641 ctacccgttc ttgtcgttga gattgttcaa ttttctgcgt cattttgatt gccttattct
    11701 tcgcggatgc cggaaagttc tgcacgggag agcaccagac gggcacgatc gataaccgga
    11761 ccgtccacca tcttgccgtt cagggaaacc acgccgaggc cttcgcgagc ggcggcttca
    11821 gcggcttcta cgacgcggcg ggcgtgatcc acttctttct gggtcggtgc gtagaggttg
    11881 tgcaacagat caatctgacg cgggttgatc agcgatttgc cgtcaaagcc cagctgtttg
    11941 atgtgggcgg cttcttgcag gaatccggct tcgttgttag cgtcggaata gacggtatcg
    12001 aacgcctgaa tacccgcaga gcgcgcggcc tgcaaaatgg aacagcgtgc gaacagcagt
    12061 tcagttcctt ccggggagcg ttctgtacgc aggttgcgca catagtcttc tgcaccgagg
    12121 gcgataccga tcaaacgctc ggaagcgtga gcgatttcca ctgcgcgggt aatccccagc
    12181 ggagattcaa tcgccgccag caggccggtg ctgccgggtt cacgaccaca ggctttttcg
    12241 atacgcagga tctctttttc gatatccaga acatcctgag cggtatcggt tttcggcaga
    12301 cgcacaacgt ccgcaccacc gcgaacgacg gcttccaggt cgttgacacc ccattcggaa
    12361 tccagcgcgt tgacacgcac aatggtttca atatcgcgat acagcggatg ttgcagcgcg
    12421 tggtaaacca tgcggcgggc ggtgtctttt tcacgcaatg ctacggagtc ttcgaggtca
    12481 aacatcaggg catcagccgg gtagatgaag gagttgctga ccatcgcggc attggcacca
    12541 ggcacaaaca acatgctgcg gcgggtgcga gttttacgtt gttgcagcga agcggaaatc
    12601 attggcaatc ctcccatggc agagccggga taccgctggc gcgtgccagc agggcttcca
    12661 gtcgtgcacg taaaatgcag tccagtgcgc ctttgtcatc gacattcagc tgtacgccgc
    12721 gcacgttgta gcgggcgaga acgtccagaa tggtggtgcg aattgcatcg ccaaactgtt
    12781 tctcaacgct gctattgatt tgcaggtcga tatcctgcgt atcgagtggg gcgatgcgta
    12841 tcatcacatc cccagactca agggtgcctg caacggcggg ctggtttatt ttcatttttc
    12901 acctgtttct catgcggggg tcttttgacg agctgccgcg tcctggcggg agtgctcaag
    12961 caggttctgc aaataatgca gcgtgactgc agggaccagc ggcgcgatag ccgtgagatc
    13021 gtttttcgcc agcagttgac gtacccggga agcggatatc ggcatctcct ggtaacgcag
    13081 ccgctcaatt tcaaccagtt cgatgggcgg tgcggagata gtcggcgttt ccagccagta
    13141 gcgcatatcc tggttgtact gggcggtaac gcgacaaaag ggttcagtac cgacaaagcg
    13201 gtgagttaca cccagcgcgg gagcgaggta ctgacggaaa atcttcagat caatttcggt
    13261 gtaacaatgg ttaatgacgc tctgttcttt aatgaagtag caagggaacg tagcgcggga
    13321 gatgatgtat tcggagccgc gatgcacagt caggcgtgga atatcggcgg tgccttttaa
    13381 caccagatcc aaccggtctt cataggggaa gcgtgaagaa tcttctttga ctaaaaacag
    13441 atgcaaccag tcgcactgtg ccgcagcttg ttgaatcaga taacggtgac cattcgtgaa
    13501 gggattggcg ttcatcacaa tgcagccaat cttgttccct ggatgacgaa attttttcag
    13561 cgattcggca tagcgtttca gccgcgtggc gctgttttcc atcagcacca tcacgccggg
    13621 tacgctggtc agcgtggaaa aaccgcactg gcggaacagc gcctcgtatt cggttttggt
    13681 ataaataaac agatgcgtgc tgtgccgctc ataggcgagg tttatcaatt cagtggctaa
    13741 tgtcagcgcc agtccttcac cgcgaacgga ttcactgata gcaacgcatt taataatatt
    13801 tccggca
//
LOCUS       DAETQN010000116        13794 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.116, whole genome shotgun sequence.
ACCESSION   DAETQN010000116 DAETQN010000000
VERSION     DAETQN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.116"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..147)
                     /locus_tag="K0W68_003367"
     CDS             complement(<1..147)
                     /locus_tag="K0W68_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBI3074836.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKA"
     gene            complement(344..475)
                     /locus_tag="K0W68_003368"
                     /pseudo
     CDS             complement(344..475)
                     /locus_tag="K0W68_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            476..1021
                     /locus_tag="K0W68_003369"
                     /pseudo
     CDS             476..1021
                     /locus_tag="K0W68_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1063..2064)
                     /gene="gap"
                     /locus_tag="K0W68_003370"
     CDS             complement(1063..2064)
                     /gene="gap"
                     /locus_tag="K0W68_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBI3074837.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2253..2783
                     /gene="cybB"
                     /locus_tag="K0W68_003371"
     CDS             2253..2783
                     /gene="cybB"
                     /locus_tag="K0W68_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBI3074838.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3028..3201
                     /locus_tag="K0W68_003372"
     CDS             3028..3201
                     /locus_tag="K0W68_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074839.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="K0W68_003373"
     CDS             complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="K0W68_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBI3074840.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3821..5461
                     /gene="trg"
                     /locus_tag="K0W68_003374"
     CDS             3821..5461
                     /gene="trg"
                     /locus_tag="K0W68_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBI3074841.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5499..6422)
                     /locus_tag="K0W68_003375"
     CDS             complement(5499..6422)
                     /locus_tag="K0W68_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3074842.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6639..7982
                     /locus_tag="K0W68_003376"
     CDS             6639..7982
                     /locus_tag="K0W68_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBI3074843.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8207..9862
                     /gene="opgD"
                     /locus_tag="K0W68_003377"
                     /gene_synonym="mdoD"
     CDS             8207..9862
                     /gene="opgD"
                     /locus_tag="K0W68_003377"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBI3074844.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            10002..10226
                     /locus_tag="K0W68_003378"
     CDS             10002..10226
                     /locus_tag="K0W68_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBI3074845.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            10289..10825
                     /gene="rimL"
                     /locus_tag="K0W68_003379"
     CDS             10289..10825
                     /gene="rimL"
                     /locus_tag="K0W68_003379"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBI3074846.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(10820..11800)
                     /locus_tag="K0W68_003380"
     CDS             complement(10820..11800)
                     /locus_tag="K0W68_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HBI3074847.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            11924..12916
                     /gene="tehA"
                     /locus_tag="K0W68_003381"
     CDS             11924..12916
                     /gene="tehA"
                     /locus_tag="K0W68_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBI3074848.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            12913..13506
                     /gene="tehB"
                     /locus_tag="K0W68_003382"
     CDS             12913..13506
                     /gene="tehB"
                     /locus_tag="K0W68_003382"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBI3074849.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3540 a         3197 c         3483 g         3574 t
ORIGIN      
        1 tgccttacgg gcatcctcgg cctgaccatc gggtatacgg gaaatgacag cctctgtagc
       61 agggtttacc acatcaatcc atgcgtctcc acgccaggta acaaactgtc catcgatata
      121 cataggatgt tgaacgggta ctgacatggg cgactcctgt gatttatatg tttgttttcg
      181 gtgaatatat tgttaattaa catcaatcat cagagagacg gcatcgggaa aggacaattc
      241 tgtgaagggc ttcataaaaa caagtgtaaa aggaataaaa ggcagaaata aaagtcagga
      301 gtcatgcccg tttgcaaaca agtggttcac aaacgggcaa tttttaacgt aacgagcgac
      361 tttccatcgc ctcgatgagc accgcatcat gtttcagcgt ttgccatgca tggatgactt
      421 ctgccggaaa atcaacgtga acgataaaat ctcgcccgcg gggaccgtag ccattgatca
      481 gttcgtcaat cggctgggtg aagcgatgca ggcggttaaa tttggtaacc ccgctgaacg
      541 caacgacatt gcgatggggc cgttgattaa cgccgcggcg ctggaaaggg ttgagcaaaa
      601 agtggcgcgc gcagtagaag aaggggcgag agtggcgttg ggtggcaaag cggtagaggg
      661 gaaaggatat tattatccgc cgacattgct gctggatgtt cgccaggata tgtcgattat
      721 gcatgaggaa acctttggcc cggttctgcc ggtggtcgca tttgacacgc tggaagaggc
      781 tatctcaatg gctaatgaca gtgattacgg cctgacctca tcaatctata cccaaaatct
      841 gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt gaaacttaca tcaaccgtga
      901 aaacttcgaa gctatgcaag gcttccacgc cggatggcgt aaatccggta ttggcggcgc
      961 agatggtaaa catggcctgc atgaatatct gcagacccag gtggtttatt tacagtcgta
     1021 atgagtaaaa gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt
     1081 ttcgagggtg cgaataagct gcgtgacgaa gccatattcg ttatcgtacc aggcgaccgt
     1141 tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat caaacaccga
     1201 accgaaatgg ctgccaatga tatcggaaga gactatttct tcatcggtat aaccaaatga
     1261 ctcgttattg gtggttgctt gtttaagtgc gttattcacc tcttcggcag tcactttttt
     1321 tccgagaatc gataccagtt cagtgaccga acctgttttc accggcacgc gttgcgcatg
     1381 acctttcagt ttgccgctca gttccgggat caccagacca atggcttttg ccgcccccgt
     1441 agtgtgggga atgatatttt ctgccgctgc gcgtgaagca cgtaaatctt taccacgtgg
     1501 gccatccacc agtgactggg tgccagtata ggcatgaatg gtcgtcatcg tgccgacttc
     1561 tatcccgaaa ctgtcatgca aggctttggc catcggcgca agacagttag tggtgcatga
     1621 cgccacggaa acaatggtgt cgttgccatc cagagtgtcg tcattgacgt tataaacgat
     1681 agttttcatt tcaccggcag gggcggaaat caacaccttc ttcgcaccag catcaagatg
     1741 tgcctgcgat ttctcggcgg aggtataaaa gccagtacat tcgacaatga tttctgcacc
     1801 tttcgctttc cacggaatat ttttagcctc tttttcggcg taaaccgcga tacttttccc
     1861 atcaacgata agtgaatctt ccgtaaaatc aacgctccag gggaatggtc cgtagtttga
     1921 atcatgtttc agcaggtagg cgagaatttt tggggaagtg agatcattaa tagcgacaac
     1981 gtctatgttg cttttgactt caagtaatcg acgcaacacc agtcgaccga tacgaccaaa
     2041 accgttaata ccaactttac tcatggtttt ctcctgtcag gaacattcgg atgaaaattg
     2101 atcctttcca ggcttagacc aggattgtgg gatgggcaat ctccattctt acagtgaaac
     2161 gtaacgtact gaaaacgggt gaacaatatt taatgaaatt ttgagaaaag cccgttatgt
     2221 taacggaaaa ttatgttaaa gcaggaaatg ttatggaaaa taaatactca aggttacaaa
     2281 tcagcattca ctggctggtc tttttgctgg ttatcgcagc gtattgcgca atggagtttc
     2341 gtggtttctt cccacgtagc gatcggccac tcatcaacat gattcatgtt tcctgcggca
     2401 tctcaattct cgtgctgatg gtcgttcgtc ttttgttaag gctgaaatac ccaaccccgc
     2461 cgattatacc taagccaaaa ccgatgatga cgggactggc gcatttggga catttggtga
     2521 tgtatctcct gtttattgcg ctgccagtga ttggtttggt gatgatgtat aaccggggca
     2581 acccgtggtt tgcgtttggt ttgacgatgc cttacgcttc agaggccaat ttcgaacggg
     2641 ttgatagctt aaagtcgtgg catgagacgc tggcgaatct gggatatttt gtcatcgggt
     2701 tgcacgctgc ggctgcactg gcacaccact atttctggaa ggacaacaca cttctacgca
     2761 tgatgccgcg taaacgttcc tgaaggatat ttcaagaaaa tgcctgtact aaaaccgacc
     2821 cgtggtacag gcgaagaata cgggtctaca tcggaagcgc ctattatatt tatttgtatg
     2881 acaaataaaa ccataatcct tgcccatacg tccatctggc ttatttttaa tcaattcacc
     2941 cgatctttga tctcatcaac ggtatcaaaa taaaccagcg tataacgttc aaatcgaaca
     3001 taagattcga taaaccatgg aggttatatg aaaaaactgg cacttatttt gtttatggga
     3061 acgcttgttt ccttttatgc cgatgccggg cgcaaaccct gttctggttc gaaagggggg
     3121 atctcacact gtacggcagg cggcaaattt gtctgtaatg atggttctat tagtgcatcg
     3181 aaaaaaacat gcactaactg aagtgtaaaa ggggtgccat gagaaaatga atctgctagt
     3241 cagatgcgcg gggaaaatcc ccgcgcttgc ccttacctgg acgtgcaggc catgagcgca
     3301 gcaacctcct tatcaccgtc ccggaaccgc agttcgtaga gcgtttgtcg ggtcaggagt
     3361 gtgaatgtca gaatcgtaat gcagataatg agcagacaca ctaccagagg gttgtgcttc
     3421 atagcctttc tccttgccgg atggcgggta agaggctaag atctgaattg ctaggttcat
     3481 tcgttggcct cggttgatag aaatatcggt cggggccttc gtctttctga ttcccggtta
     3541 gcctgaaaac agaaagtctc aggcacccgc aggcatccta tgaggtttcc ttagggacga
     3601 aaataatcac ttcacgaaat tgcgtgctgt tttccagaat ttttcgtcat tcgggttagc
     3661 cagtttagcc attcgttact ctcttcattc caatagcatt aattttctct gcaataattg
     3721 ttgtaaaaat gtgacgcaaa gaggtttttg gtcataagta attaccgtca agtgccgatg
     3781 actttctatc aggagtaaac ctggacgaga gacaacggta atgaatacaa ctccctcaca
     3841 gcgattaggt tttttgcatc acatcaggct ggttccgtta tttgcctgca ttcttggcgg
     3901 tatcttagtt ctattcgcat taagttctgc tctggctggc tatttcctct ggcaggccga
     3961 tcgcgatcag cgtgatgtta ctgcggagat tgagattcgg accgggttag cgaacagttc
     4021 agattttttg cgttcagccc ggatcaatat gattcaggcc ggggctgcga gttgtattgc
     4081 ggaaatggaa gcaatgaagc gaaatattgc gcaagccgaa tcggagatta aacagtcgca
     4141 gcaagggtat cgtgcttatc agaatcggcc ggtgaaaaca cctgctgatg aagccctcga
     4201 cactgaatta aatcaacgct ttcaggctta tatcacgggt atgcaaccta tgttgaaata
     4261 tgccaaaaat ggcatgtttg aagcgattat caatcatgaa agtgagcaga tccgaccgct
     4321 ggataatgct tataccgata ttttgaacaa agccgttaag atacgtagca ccagagccaa
     4381 ccaactggcg gaactggccc atcagcgcac ccgcttgggt gggatgttca tgattggcgc
     4441 gtttgtgctt gccctggtca tgacgctgat aacatttatg gtgctacgtc ggatcgtcat
     4501 tcgtccactg caacatgccg cacaacggat tgaaaaaatc gctagtggcg atctgacgat
     4561 gaaggatgaa ccggcgggtc gtaatgaaat cggtcgctta aatcgtcatt tacagcaaat
     4621 gcagcattca ctggggatga cagtagggac tgttcgacag ggtgcggaag agatttatcg
     4681 tggcaccagc gaaatttcag ctggcaatgc ggacctgtca tctcgcaccg aagaacaagc
     4741 ggcggctatc gaacaaactg ccgccagcat ggagcaactc actgcgacgg tgaaacagaa
     4801 tgcggataac gcgcatcatg ccagcaaact ggcgcaagag gcttctatta aagccagcga
     4861 tggcgggcag acggtttccg gtgtagtaaa aacgatgggc gctatctcca cgagttcgaa
     4921 gaaaatttct gagatcaccg ccgtcatcaa cagtattgct ttccagacga atattctggc
     4981 actgaatgct gccgttgaag ccgcgcgagc gggtgagcaa gggcgtggat ttgccgttgt
     5041 cgccagcgaa gtacggacac tcgcaagtcg cagcgctcag gcggcgaaag agattgaagg
     5101 cttgatcagt gaatcagtca ggttaattga cctggggtcg gatgaggtgg caacggccgg
     5161 gaaaaccatg agcactattg ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat
     5221 cgccgccgcc tcggatgaac aaagtagagg cataacgcag gttagccagg cgatttctga
     5281 aatggataag gtgacgcaac agaatgcttc tctggtagaa gaggcctcag cggcggcggt
     5341 gtcccttgaa gaacaggcgg cacgattaac tgaggcggtg gatgtattcc gtctgcacaa
     5401 acattctgtg tcggcagaac ctcgcggagc gggtgaacca gttagtttcg ctacggtgtg
     5461 aaaatgttca aggagggatc gacagatccc ttcacctttc agaacggcat tgattttcga
     5521 atagcgttaa tcatcaactg gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt
     5581 ccaatcggtt cgcctgcacc atgtcccgga acaggcaggg ccaccagcgt ggcatgacgc
     5641 aggtcgtctt ttacagcgcc agaagggaca aaccacacgt aatcgtattc aaccgtaagt
     5701 tgacgagata gcgaagcaga cagcgtttcg atacaacccg aaggaatttt acagccctgg
     5761 ctctgcacta atgcatctga atgctggcgt ggcgcagtgc cttctggtga tacaacgacc
     5821 ggccattcca gcacccggct tagcgttacg ttctcctgaa gtagcgggtg attagggcgg
     5881 acaaccagct tcagcgattc aagaaacagc agttcgtaat taagcccggt catcagttca
     5941 ggatctgaca tccgaccaat gccgatatcg atttccccgg tttttaaacc cgccagaatc
     6001 atagggttac tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg atgaaactga
     6061 cctataaccg aaggtaatat ccccagtgcc gcagtaggta gtgcaccaac cctgacgaca
     6121 tcattattaa gaccttcttt acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga
     6181 actctgactg catgcgttaa aaattgttcg ccgggtaagg taagttgcgc cccctgacga
     6241 ccacgctcaa acaagcgagc gcccgtcagc tgctccagtt cattcaatgt cttagagagc
     6301 gcaggttgac tcaaattaag ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca
     6361 gctacgaatg tatgaaggtg gcgcaaacgg atgcgctgac taaacagact atttttttcc
     6421 ataagcgatg ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttg attatgataa
     6481 cttgattgca aaatattatt aacaattaaa gcaattatgt tacagcaaaa tggataatat
     6541 tgatgttttc gcggcgagat cacagtttgt aaattcttcc cgccagagtg aatgcggtta
     6601 cctacactcc agattactga ccactggagg cagacactat ggcgaacagc atcacggcgg
     6661 atgagattcg ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc
     6721 aatatggcac gctgctggaa ctggtagctg atgtgaatct ggctgtgctg gaaaacaatc
     6781 ctcaactgca cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat gttgaacgtc
     6841 atggggcgat tcgcgttggg actgcacaag agcttgctac tcttcggcgg atgtttgcca
     6901 ttatggggat gtacccggtg agctattacg atctctcgca ggcaggggtg ccggtacatt
     6961 cgacagcatt tcggcccatt gatgatgctt ctctggcgcg taatcccttc cgcgttttta
     7021 cctccttact ccgccttgag cttatcgaga acgaaatttt gcgccagaaa gcggcggaga
     7081 ttctgcgtca gcgcgatatc ttcaccccac gttgtcgaca actgttagag gaatatgagc
     7141 agcagggcgg ttttaacgaa acacaggcac aggagtttgt gcaggaagcc ctggaaacgt
     7201 ttcgctggca ccagtcagca acggtagatg aagaaaccta tcgcgccttg cacaacgaac
     7261 atcggttgat tgctgatgtg gtctgttttc ctggatgcca tatcaaccac ctgacgccac
     7321 gtacgctgga tattgaccgg gtgcagtcga tgatgcctga atgcggaatt gaacccaaaa
     7381 ttctcatcga ggggccgccg cgccgcgagg taccgatttt actacgccag accagcttta
     7441 aagcactgga agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct
     7501 ttggtgaaat tgagcagcgt ggcgtggcat taacgccgaa agggcgacaa ctgtatgatg
     7561 atcttctgcg taacgctgga accgggcagg ataatctcac tcaccaaatg catttacagg
     7621 aaaccttccg cacttttcct gacagtgagt ttttaatgcg tcagcaaggg ttggcatggt
     7681 tccggtaccg tctgacgcct tcgggtgagg cgcatcgtca ggcgattcat cctggagacg
     7741 atccacagcc cttaattgaa cgtggttggg tagtggcgca acccatcacc tatgaagatt
     7801 tcttgcccgt tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa acgcagacac
     7861 gcagtcacgg taatgccagt cgcgaagcat ttgagcaggc gttgggttgt ccggttttgg
     7921 atgagttcca gctttaccag gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt
     7981 aaattctgac catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat
     8041 cccatcgaca taaaaaaaat gccgatttat gcatattctc tcagttcaac aattggatta
     8101 ttaataaata ttgtctagag tgagcggtca taaataagca ctttcttgcc gctgaaaacg
     8161 accagcgcgg gaccattcac aacaccagaa ggactcactt tcaggtatgg atcgtagacg
     8221 atttattaaa ggttcaatgg ctatggccgc cgtgtgcggt accagcggca ttgcttctct
     8281 tttttctcag gcggcattcg cggcagattc tgatattgcc gacgggcaaa cccagcgttt
     8341 tgacttctcc attctacagt caatggcgca cgacttagcg caaacagcgt ggcgtggtgc
     8401 gcctcgtccg ttacctgaca cgctggcgac aatgacgccg caggcttata acagtattca
     8461 atacgacgcc gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg acgctcagtt
     8521 cttccatatg ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag atccagcaac
     8581 acatctggcg cgtgaaattc actttcgccc ggagttgttc aaatacaacg atgcaggtgt
     8641 tgataccaaa caattagaag ggcaaagcga tctcggcttt gccggttttc gcgtgtttaa
     8701 agcccccgaa ctggcgcgcc gtgatgtagt atcatttctc ggcgcgagtt atttccgcgc
     8761 cgttgatgat acatatcaat acggtttgtc ggcccgcggc ctggcgatcg acacttacac
     8821 cgacagtaaa gaagagttcc ccgactttac cgccttctgg tttgatacgg taaaaccggg
     8881 ggcaactacc tttaccgttt atgcgttgct cgatagcgcc agtattactg gtgcctataa
     8941 gttcactatc cattgtgaga aaagtcaggt gattatggat gtggaaaatc acctgtatgc
     9001 gcgcaaagac attaaacagc tgggcattgc gccgatgacc agtatgttca gctgcggtac
     9061 taatgaacgt cggatgtgcg acaccattca tccgcaaatt catgactctg atcgtctgtc
     9121 catgtggcgg ggcaacggcg agtggatttg ccgtccgctg aacaatccgc aaaaattgca
     9181 gttcaatgct tacaccgaca acaacccgaa agggtttggt ttattgcaac tggatcgtga
     9241 cttctcccat tatcaggaca ttatgggctg gtataacaaa cgcccaagtt tgtgggtgga
     9301 accgcgtaac aagtggggta agggcaccat cggcctgatg gaaatcccaa caacgggcga
     9361 aacgctggat aacattgtct gcttctggca gccagaaaaa gctgtaaaag caggtgatga
     9421 gtttgcattc cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt gcccattagc
     9481 gcgcgttatg gcgacgcgta ccggcatggg cggtttcccg gaaggttggg cgccaggtga
     9541 acactatccc gaaaaatggg cgcgtcgttt tgccgtcgat ttcgttggtg gtgatctgaa
     9601 agctgccgcg ccaaaaggca ttgagccggt gattacgctt tccagtgggg aagcgaagca
     9661 aatcgaaatt ctctatattg aacccatcga tggttatcgt attcagtttg actggtatcc
     9721 gacttcggac tctactgatc cggtcgatat gcggatgtat ctacgttgtc agggggacgc
     9781 tatcagtgaa acatggctgt atcagtattt cccgccagcg ccggataaac gtcagtatgt
     9841 tgacgaccgc gtgatgagtt aatcgttttt tcttcggcac cttcttcggg aggtgccgtc
     9901 tggttaaaca cgatcccgct cgcatttttc cctaagttaa atgagtaatc tgatggtgtg
     9961 tatttcagat acaccttgtc agccactaac agggagtgcg tatgtttcca gaataccgag
    10021 atttaatatc ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc gataaacaca
    10081 ataaacttga tcatgaaatt gccagaaagg aaggttccga cggtcgaggg tacaatgcgg
    10141 aagtggtccg catgaaaaaa caaaagctac agttaaaaga tgagatgctc aaaatcctgc
    10201 agcaggagag cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc ggctttttta
    10261 acaacagcat ggattgactg gagataagat gactgaaacg ataaaagtaa gcgaatcact
    10321 tgaattacat gctgttgcgg aaagtcacgt cacacctctt tatcagttaa tctgtaaaaa
    10381 taaaacctgg ttacagcagt cgctaaactg gccgcagttc gttcagagtg aagaggacac
    10441 gcgaaaaacg gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat
    10501 gattttcaaa gaagatgaac ttatcggcgt tatctcgttt aatcgtattg aaccactgaa
    10561 taaaactgct gaaatcggct actggctgga cgaatctcat caggggcagg ggatcatttc
    10621 tcaggcgctg caggcattga ttcatcatta cgctcagtct ggtgaactta gacgcttcgt
    10681 gatcaaatgt cgggtggaca atccgcaaag caaccaggtc gctttgcgca atggttttat
    10741 ccttgaaggt tgcctgaaac aggctgagtt cctgaatgat gcctatgatg atgtgaactt
    10801 atacgcgcgt attatcgatt cataataacc ctgccagcgg cgttcgcgta atgcgctcct
    10861 cgccgttaat gacttttgga ccacggacat gaaccgtgtc gccatcaaag gcttcaacga
    10921 cggcgtggtc ggttaactcc acatggtttt caattatcac cgcacctgaa atacggctct
    10981 caccttgtat tacaacgtgc tcgtcgagca gaatcggtcc accacgtacc acggcatttc
    11041 cgccaatcaa gacatgatgt tttaacacac aattaccttc cacaatggca tattccgcca
    11101 cctgcgaact gtaatgaatc gtcggaatgg catcttcttc tatgccagct ttcacctgcg
    11161 cgtgaccgta gacttttgcg caatcgcata gccagacgtt gttctcttca ttaccttcaa
    11221 cactggcaaa atcaaaaact tcggcgcgat gttcaataaa agcataccgg acgacggcat
    11281 cgccataaat ttgtgcctgg tggacaatac gcgaggcgct aacccttgcg cgatcataaa
    11341 tttggagcag gagttgatgg tcgggcgtta agccttgtgc ggcgacgatc atagaatgtt
    11401 gatcaattaa ggcgtgacca aatattctac attgcccgta aactaatgag tcacgaatgg
    11461 tgacactgtc acttatatat gcaccctggc tgatttcact attatcgatc cagacgttat
    11521 ccgttgcgta aacctctccc cacagcacgc tggtgccggt gatacgcgta ttaccagaaa
    11581 tcaccgttcc gccgaacgca atagcattct ggtcgtaaat ccagcaattt ccttcctgtg
    11641 ctaacaccgt ttcgcgatcg atccagccgc cagcggttcc tgctatcaca tcgttaaaat
    11701 cgctaatggc gataatctgc cgtaataaca cacttttttt agtgccatct tcctgataac
    11761 taaaggctcg ctgttcttca cttagacgat atttgcgcat agcgttttct cacaggtgac
    11821 ttactataac cgtagcaaat tctgcggctc tggctatgct cgagaaaatt ccataaaatg
    11881 catttcaaat atactttata aattaaacaa aatgagtaag aagatgcaga gcgataaagt
    11941 gctcaatttg ccggcaggct actttggtat tgtgttgggg acgataggga tgggatttgc
    12001 ctggcgctat gccagccagg tttggcaggt cagccactgg ttaggggatg ggctggtgat
    12061 tctggcgatg atcatctggg gattattaac tagcgcattt attacccgac tcatacgctt
    12121 tccgcatagc gtgctggcgg aagttcgcca tccagtgctg agcagttttg tgagtttgtt
    12181 tccggcaacg acgatgctgg tggcgattgg ttttgttccg tggtttcgcc cactggcggt
    12241 gtgcctgttc agttttggtg tcgtggttca gttggcttat gccgcctggc aaactgcggg
    12301 attatggcgc ggatctcacc ctgaagaagc taccacgcct ggactgtatc tgccgacagt
    12361 tgccaacaac tttatcagcg caatggcctg tggtgcgttg ggctacaccg acgccggtct
    12421 ggtgttttta ggcgcaggcg ttttctcatg gctaagtctt gaaccggtga tcttgcagcg
    12481 tctgcgcagt tcgggagaat tacccacggc actgcggaca tcactcggca ttcagctcgc
    12541 tcctgcgctg gtggcctgta gtgcctggtt gagcgtcaac ggcggcgagg gtgatacgct
    12601 ggcgaaaatg ctttttggtt atggactgct gcaactgctg tttatgctac gtctgatgcc
    12661 atggtatctc tcccagccat ttaatgcttc attctggagt ttctcgttcg gcgtatctgc
    12721 actggcaacc accggtttgc atctggggag tggcagcgaa aatggatttt tccatacgct
    12781 ggcggtaccg ctgtttatct ttaccaattt tattattgca atactgctca tccgtacttt
    12841 tgcgcttctg atgcagggaa aattgttagt cagaaccgag cgcgccgttt taatgaaagc
    12901 agaggacaaa gaatgatcat tcgtgacgaa aactatttta ctgataaata tgaattaacc
    12961 cgcacacact ctgaagtact ggaagcggtg aaagtggtta aaccgggtaa aacgctggat
    13021 ctgggctgtg gcaatggtcg taacagtctt tacctggcag ccaatggtta tgatgttgac
    13081 gcatgggata aaaatgccat gagtatcgcc aatgtagagc gcattaaatc cattgaaaat
    13141 ctggataatt tacacacccg agtcgttgat ctgaataacc tcacatttga tagacagtac
    13201 gattttattc tttcgactgt ggtactgatg ttccttgagg ctaaaaccat cccagggctg
    13261 attgccaata tgcaacgttg cactaaacct ggtggttaca acctgattgt ggcggcgatg
    13321 gataccgctg attatccatg taccgtcggc ttcccgtttg ccttcaaaga gggagaatta
    13381 cgtcgatatt acgaaggctg ggagagggtg aaatacaatg aagacgtcgg cgagctgcac
    13441 cgcaccgacg ccaacggtaa tcgtattaaa ctgcgtttcg ccacgatgct ggcacgtaaa
    13501 aaatgacccg ctaagaataa ggcgcgtgaa aattccccac gctgagatga tttactgttc
    13561 ttcttttcgg taagcatatt ttttatcgaa gggatgtgaa attaatcaca gtagtcgaag
    13621 ttttttagca gcttaactta ctgaaattta actactgatg attgacttag cccctttttc
    13681 ggcattgact atgtcgtctg aaaaggggct gaaaaattta ttttcaccaa cacttttttt
    13741 gccacaacac gaagcggcgc tttttgctat aacttaaaaa gtaatataat catc
//
LOCUS       DAETQN010000117        13780 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.117, whole genome shotgun sequence.
ACCESSION   DAETQN010000117 DAETQN010000000
VERSION     DAETQN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13780
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.117"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1138
                     /locus_tag="K0W68_003383"
     CDS             <1..1138
                     /locus_tag="K0W68_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBI3074850.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIY
                     WNSRASLFCSTELNSKSQSPALGLGHEFTHAQYCLLDKENFMALLSRTDKKYENKEEA
                     RVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            1150..1599
                     /locus_tag="K0W68_003384"
     CDS             1150..1599
                     /locus_tag="K0W68_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074851.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
     gene            1861..2997
                     /locus_tag="K0W68_003385"
                     /pseudo
     CDS             1861..2997
                     /locus_tag="K0W68_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            3167..3445
                     /locus_tag="K0W68_003386"
     CDS             3167..3445
                     /locus_tag="K0W68_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HBI3074852.1"
                     /translation="MGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRYELATGEKV
                     GGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
     gene            3445..3822
                     /locus_tag="K0W68_003387"
     CDS             3445..3822
                     /locus_tag="K0W68_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074853.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            4291..5427
                     /locus_tag="K0W68_003388"
     CDS             4291..5427
                     /locus_tag="K0W68_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBI3074854.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(5469..6239)
                     /gene="yafV"
                     /locus_tag="K0W68_003389"
     CDS             complement(5469..6239)
                     /gene="yafV"
                     /locus_tag="K0W68_003389"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBI3074855.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVAWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            6420..6866
                     /gene="ivy"
                     /locus_tag="K0W68_003390"
     CDS             6420..6866
                     /gene="ivy"
                     /locus_tag="K0W68_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBI3074856.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(6909..9353)
                     /gene="fadE"
                     /locus_tag="K0W68_003391"
     CDS             complement(6909..9353)
                     /gene="fadE"
                     /locus_tag="K0W68_003391"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HBI3074857.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            9593..10171
                     /gene="lpcA"
                     /locus_tag="K0W68_003392"
     CDS             9593..10171
                     /gene="lpcA"
                     /locus_tag="K0W68_003392"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBI3074858.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            10377..11144
                     /locus_tag="K0W68_003393"
     CDS             10377..11144
                     /locus_tag="K0W68_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBI3074859.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(11115..11855)
                     /locus_tag="K0W68_003394"
     CDS             complement(11115..11855)
                     /locus_tag="K0W68_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBI3074860.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(12011..12289)
                     /gene="yafQ"
                     /locus_tag="K0W68_003395"
     CDS             complement(12011..12289)
                     /gene="yafQ"
                     /locus_tag="K0W68_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HBI3074861.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(12292..12552)
                     /gene="dinJ"
                     /locus_tag="K0W68_003396"
     CDS             complement(12292..12552)
                     /gene="dinJ"
                     /locus_tag="K0W68_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HBI3074862.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            12738..13511
                     /locus_tag="K0W68_003397"
     CDS             12738..13511
                     /locus_tag="K0W68_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBI3074863.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFACATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT         3784 a         3092 c         3451 g         3453 t
ORIGIN      
        1 gacatggtac gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat
       61 ccagacggta taccagccgg ggagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggtgt
      181 gacgctaccg gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcgcgcggg
      241 agcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg cggagcagat
      301 ggccgcgcag ctggaggcgg aatacatccc ggagaggaaa cttcatcttt accactgcga
      361 ccaccgggga ctgccgctgg cgctcatcag cccggaaggg gaaacggcgt ggcaggggga
      421 gtatgacgaa tggggaaacc tgctgggcga aaccagcgcg cagcacctcc agcagtcact
      481 ccgtctgccg gggcagcagt atgatgagga gtcggggctg tactacaacc gcaaccggta
      541 ctatgacccg ttgcagggaa ggtatatcac ccaggatccg atagggctga gaggtgaatg
      601 gaatctgtat aaatatccat taaaccctgt tcggtttata gattctttgg gtttaaaatt
      661 ccatgttaac ggtgatccaa gtgattttaa tcaggctgtt gagtatttaa aacaagattc
      721 acaaatgaaa gaaactatag attttttatc aagctctgaa gaaactataa atatagagta
      781 tattgaagga accaatgtta gatttaattc gaataatatg acgatatatt ggaatagtag
      841 agcatcttta ttttgttcaa ctgaattaaa ctcgaaatca caatcgcctg cattaggttt
      901 gggacatgag tttactcatg cgcagtattg ccttcttgat aaggaaaatt tcatggctct
      961 tttaagccga acagataaga aatatgagaa taaagaagag gcaagggtta ttacaataat
     1021 tgaatcgcgt gcggcgaaaa ctttaggtga gtgtaccaga ggagctcata gtggactgcc
     1081 tttttatcgg gtggatggac cgttgcagac gatgaaaata acgggtacgc cagaatagtg
     1141 aggtttttta tgatgaaata tttgatggtt ttattgtctc tattttctgg ttcggtattg
     1201 gggatggggc gagtgaatga attgtgtgga atagatagcg tgaaaacgat agagataatt
     1261 aatcttccat cgtatgttac cacgttagtt ccgcttagca aggaaggtct taatgaaata
     1321 tacagatata aagtggtggt gaatgaaatt agagatcttt atgctggtaa gataattgat
     1381 ttattgcaaa tgaagtattt cagaaaagaa aaatataaca atataagatg ggggggtagt
     1441 atcatttcga aaggtaataa taaatgtgaa atttattttg atgcttttgg tgaatgtgga
     1501 agtgtgaatg gcattaacgt gtgttttgaa aaaaatgaaa tgataggttg gattaaaaaa
     1561 gaaatccctt tgctgtcgca aaaaataggt gggctttaaa tggtagggca atattcaaag
     1621 acagtatagg taactatttg tatggccatg taaggtaaaa gaaaaggaat aaaccatgag
     1681 cggaaaaccg gcggcgcggc agggcgacat gacccgcagg gaaagatcat cagaagttca
     1741 ctttttgtac taaataattc gcattttatg tttaaaaatt gagatattcc ttattaccta
     1801 aagctgtttt ttattgctta tacatgatca aatacttctt acataattaa ggagaacaaa
     1861 atggaactta aaaaattgat ggaacatatt tctattatcc ccgattacag acaagcctgg
     1921 aaagtggaac ataaattatc ggatattcta ctgttgacta tttgtgccgt tatttctggt
     1981 gcagagggtt gggaagatat agaggatttt ggggaaacac atctcgattt tttgaagcaa
     2041 tatggtgatt ttgaaaatgg tattcctgtt cacgatacca ttgccagagt tgtatcctgt
     2101 atcagtcctg caaaatttca cgagtgcttt attaactgaa tgcgtgactg ccattcttca
     2161 gatgataaag acgtcattgc aattgatgga aaaacgctcc ggcactctta tgacaagagt
     2221 cgccgcaggg gagcgattca tgtcattagt gcgttctcaa caatgcacag tctggtcatc
     2281 ggacagatca agacggatga gaaatctaat gagattacag ctatcccaga acttcttaac
     2341 atgctagata ttaaaggaaa aatcatcaca actgatgcga tgggttgcca gaaagatatt
     2401 gcagagaaga tacaaaaaca gggaggtgat tatttattcg cggtaaaagg aaaccagggg
     2461 cggctaaata aagcctttga ggaaaaattt ccgctgaaag aattaaataa tccagagcat
     2521 gacagttacg caatgagtga aaagagtcac ggcagagaag aaatccgtct tcatattgtt
     2581 tgcgatgtcc ctgatgaact tattgatttc acgtttgaat ggaaaggact gaagaaatta
     2641 tgcgtggcag tctcctttcg gtcaataata gcagaacaaa agaaagagcc agaaatgacg
     2701 gtcagatatt atatcagttc tgctgattta accgcagaaa agttcgccac agcaatccga
     2761 aaccactggc acgtggagaa taagctgcac tggcgtctgg acgtggtaat gaatgaagac
     2821 gactgcaaaa taagaagagg aaacgccgca gaattatttt cagggatacg gcacatcgct
     2881 attaatattt taacgaatga taaggtattc aaggcagggt taagacgtaa gatgcgaaaa
     2941 gcagccatgg atagaaacta tctcgcgtca gtccttgcgg ggagcgggct ttcgtaatct
     3001 ttccctaggg agtcagccga atgttgggaa ggatttgacg gatgaagaga agaactgctt
     3061 ggccggcggc agtacgggaa gcccgggagg atggggacca gaagatgaga atagcgcaag
     3121 ggatcgtatg cagcatgaac ggtataaaga tgaattgcga cagaaaatgg gaaaacccga
     3181 cgttacagat tcaaatttga aaaatattat agacgatctt tatcggccaa atgcaaaagt
     3241 aggaagtgga agcacagcag atgctgttcg ttatgaattg gccacaggtg aaaaagttgg
     3301 tggtagagga catgtattga aagcacagga ttatacaagg gctttacagg attggttgga
     3361 taagaatcca acagcatctc caggggatcg agcagcagca gaaaacgttc ttcaggattt
     3421 acagaatgct ttgggaggca agtaatgaac caatatattg ctagttatat agaaaaaatg
     3481 gcatcatttt ccactgtttt gtctgagaca gtctcattag ctaatgaata ctggcatcca
     3541 gaaacgccac cggtggttat gttgttctct caggttggaa aaacgattgt aaaaaatttt
     3601 tatgaaatgg gggatattga aagagaaaaa atatttaaac atgtcgaaga tggtatggtc
     3661 tcgcttgatg atgaattggc cacagttgta gctacagggc ttatagaggc aatagtcact
     3721 gcgaccgata gcaattcttt actttggcaa gatttaagta aatttctggg tgacaaatca
     3781 aagtctcatg cattagtgtg gaaaaatttt ggtcaggagt gatattattc aagaagtatt
     3841 aagctcgcta ttgagatctt aatgcttctt gagattaaat taaagttagg acgaagtaat
     3901 tgcaggtgga aataaatcca tagtgacgtg tggcaatggc tattagatgt tatggtgtga
     3961 tgggctttgg tgatttaaga gctaagttag attgccttat gaaaaagtat ggttatatat
     4021 ttaatgatgt gtatcatata tttaaataaa aaacggttct ctcttgtcaa gatagtaata
     4081 ggatagacta ttgtgttgat aaatataatt aagagtctac tttgttgatt ttagaagact
     4141 ttggagcagg gaaagatcat cggaagtaca ctttttgtac taaataattc gcgctaaatg
     4201 ttcaataatt gagatattcc ttattaccta aaactatttt tcatagctta tatatgatca
     4261 aatactcctt gcataaataa ggagaacaaa atggaactta aaaaactgat ggaacatatt
     4321 tctattatcc ccgattacag acaagcctgg aaagtagaac ataaattgtc agatattcta
     4381 ctgttgacta tttgtgccgt catttctggt gcagagggtt gggaagatat agaggatttt
     4441 ggtgaaacac atctcgattt tttgaagcaa tatggtgatt ttgaaaatgg tattcctgtt
     4501 cacgatacta ttgccagagt tgtatcctgt atcagtcctg caaaatttca cgagtgcttt
     4561 attaactgga tgcgtgactg ccattcttca gatgataaag acgtcattgc aattgatgga
     4621 aaaacgctcc ggcactctta tgacaagagt cgccgcaagg gagcgattca tgtcattagt
     4681 gcgttctcaa caatgcacag tctggttctc gggcagatta agacggatga gaaatccaat
     4741 gagattacag ccattcctga acttcttaac atgctggata ttaaaggaaa aatcatcaca
     4801 actgatgcga tgggatgcca gaaagatatt gcagagaaga tacaaaaaca gggcggtgat
     4861 tatttattcg cggtaaaagg aaaccagggg cggcttaata aagcctttga ggaaaaattt
     4921 ccgctgaaag aattaaataa tccagagcat gacagttacg caatcagtga aaagagtcac
     4981 ggcagagaag aaatccgtct tcatattgtt tgcgatgtcc ctgatgaact tattgatttc
     5041 acgtttgaat ggaaagggct gaagaaatta tgcgtggcag tctcctttcg gtccataata
     5101 gcagaacaaa agaaagagcc agaaatgacg gtcagatatt atatcagttc tgctgattta
     5161 accgcagaga agttcgccac agcaatccga aaccactggc acgtggagaa taagctgcac
     5221 tggcgtctgg acgtggtaat gaatgaagac gactgcaaaa taagaagagg aaatgcagca
     5281 gaattatttt cagggatacg gcacatcgct attaatattt tgacgaatga taaggtattc
     5341 aaggcagggt taagacgtaa gatgcgaaaa gcagctatgg acagaaacta tctggcgtca
     5401 gtccttgcgg agagcgggct ttcgtagtct taccccgact ctcccccagc cttaaacaca
     5461 acccccactc accgcaacct aaactcatcc gcatcctgcc atgccggaaa cttttctcta
     5521 tattcccgca atgccatcat cgacagctcc gcatcaatgc gcgttgcctg atgcgcgtcg
     5581 gcagtagcga taatctcgcc ttgtggatta atcacccggc tgtcaccgcg ataatggcag
     5641 ccgttgccat cgctgccgac gcgattgcat cccgccacat acgcctgatt ctcaatcgcg
     5701 cgggccgtca gcaatgcctg ccagtgcaga gagcgcggag caggccagtt ggcaacgtag
     5761 atggcgaggt cataatcgtt gagattgcgc gaccacaccg gaaaacgtaa gtcgtagcac
     5821 accaacggca aaatacgcca gccgcgccat gccacaatca ctcgcgcatt gcccgcttta
     5881 taatgtagat gctcatctgc catgcggaac agatgacgct tatcataaaa atgtaccgtg
     5941 ccgcccggct caaccagcaa aaagcggtta accgaaccag actccgtttg taatgcaaca
     6001 ctgcctgcaa tcagcgcatt gcactgctgc gccttagctg tcatccagtc cactacgtca
     6061 tcttgtgcta gcgacgaagc tgccgcttcc atggcaaagc cgctggtaaa catcttcggt
     6121 agaacgatca catcgcgccc ggtaatacct tccagttgac gatcaaaatg acgcaggttg
     6181 gcagggccat ccatccacac cagtggttgc tgcaaaagcg taatcttcaa accaggcacg
     6241 gtgtacaact cctttatgcg aagggtttta taactttaac accttatcag gcagttgcct
     6301 tagcgcagaa taaattgata acaaatgctg atattggaaa tatctgattt gcaaattatc
     6361 gtgttatcgc caggctttag aaggttaata acatgggcag gataagctcg ggaggaatga
     6421 tgtttaagac aataacgaca gtcgccgctc tggtcatcgc caccagtgca atggcgcagg
     6481 atgatttaac cattagcagc cttgcaaagg gcgaaaccac caaagctgca tttaatcaga
     6541 tggtacaagg gcataagctg cctgcctggg tgatgaaagg cggtacttat actcccgcac
     6601 aaaccgtaac gttgggagat gatacgtatc aggtgatgag cgcgtgcaaa ccgcatgact
     6661 gtggctcgca acgtatcgct gtgatgtggt ccgagaaatc taatcagatg acggggctgt
     6721 tctcgactat tgatgagaaa acgtcgcaag agaaactcac ctggctgaat gtgaacgatg
     6781 cgctttcgat tgatggtaaa acggtgctgt tcgcggcgtt gaccggcagc ctggaaaacc
     6841 atccggatgg ctttaatttt aaataattag cggataaaga aacggagcct ttcggctccg
     6901 ttattcaatt acgcggcttc aactttccgc actttctccg gcaactttac cggcttcgtc
     6961 gccagctctt ccggatcaaa gtcatcaacg ttaatactgc gcagacggct ttcttcagct
     7021 ttcaccagaa tagcggcttc atctttatca atcagcccct tcgccagcgc gttgtgcgcc
     7081 agttcatcca gacgggtaaa cggcaggttt ttacccagct ctttacagat ccgctgatga
     7141 attgggtcgg cggcaatcac atccaccagc gcctcttcca gcaagccaac cggattatgc
     7201 tcgctcggcg tcaggtactg accgcgacca atgcgggaac gggtggcgtt cggcacttgt
     7261 aaaatcttcg ccactttatg atccagcttg tcagaaggtg ccagataatg acgtccggtc
     7321 gggaagatca ccacattcag cagcccggca accacgcggt tcgggaagtt ttgcagtaaa
     7381 tcatccatcg cctgttcagc ctgatacagc gcatcttgta cgccccagtg caccagcggc
     7441 aggtcggctt cattacggcc ttcgtcgtca taacgcttca gcacggcaga ggcgaggtag
     7501 agctggctta aaatatcccc cagacgggcc gagatgcgct cgcgacgttt caggctgccg
     7561 tccagcactg ccatcgagac atcagaaagc agggcgaggt tggcgctcag gcggttcagg
     7621 tgctgatagt agcgtttagt ggcatcgccg gttggcgtgc tgctggttaa accgcgcgtc
     7681 aggcccagcc agaagctgcg aactttgttg ctaccgacgt gaccgatatg tttgaacaac
     7741 agtttatcga acgcgttgac gtcattgttc ttcgccgctt ccatctcttc cagcacgtac
     7801 ggatggcaac gaatcgctcc ttgtccgaag atcatcatgc tgcgggtcag aatgttagcc
     7861 ccttcaacgg tgatggcaat cggtgcgccc tggtaagcac gcgccaggaa gttgctttgc
     7921 ccgagcataa tgcctttgcc accagtgata tccatcgcat caataatcga ctgctgcccg
     7981 cggtgggtac agtgatactt aacgatagcc gacagcacgg caggtttttc gccgagcata
     8041 atgccgtagg taatcagcga tgccgcagca tccatcacgt aggcattacc ggcaatacgc
     8101 gccagcggct cttcaatccc ttccatctta ccgatagaga ttttgaactg acggcgaatg
     8161 tgagcatacg cgcccgttgc cagcgctacc gatttcacgc cgccggttga gttggaaggc
     8221 agggtgatgc cgcggcctac cgagaggcac tccaccagca tccgccagcc ttgcccggcc
     8281 attttcggcc caccgatgat gtaatcgatc ggtacgaaga catctttacc gcgcgtcggt
     8341 ccgttctgga acggtacgtt cagcgggaag tggcgacgac caatttccac gcccggcgtg
     8401 gtggtcggga tcagcgcaca ggtaatacct aaatcttctg cgccacccag cagcttttct
     8461 gggtcggaga gtttaaacgc cagcccaagc acagtcgcaa tcggtgccag cgtaatatag
     8521 cgtttgttcc aggtcagacg catccccagc acctgctggc cctgccattc gcccatgcag
     8581 acaatcccgg tgtccggaat cgcgcccgca tcggaacccg cttccgggct tgtcagtgca
     8641 aagcaaggga tctcctgacc acgcgccaga cgcggcagat agtgatcttt ctgctcgtca
     8701 gtgccgtaat gttgcagcag ctcgcccggg cctaatgagt tcggcacgcc gacggtaatc
     8761 gccaggatcc cgctcacgcc ggagagtttt tgcagcacgc gagactgggc ataagctgag
     8821 aactccagtc cgccgtactc ttttttgata atcatcgcga agaagcgatg ctctttaagg
     8881 tacgcccaca actccggcgg cagatccgcc agctcatgag taatctggaa atcattcgcc
     8941 atccggcagg cttcttctac cgggccgtcg agaaacgctt gctcttcggc ggtcaggcgc
     9001 ggctgcggat agttatgcag ctttttccag tccggcttgc cctggaacaa gtcgccctcc
     9061 caccaggtgg tgcccgcatc aatcgcttct ttctcagtgc gcgacatcgg cggcatcacc
     9121 ttacggaaac cgcgaaatac cggcgcggaa atcatcgact tacgcatagg cgcaaagtta
     9181 aatggcacga ggataatggc cagaggcacc agtacccacg ctgaccacag accagcaacg
     9241 ccgagggcgg ctgtccaggc gagcaaaatc agactgctga taaataagct cacgcggtga
     9301 tagaacaacg cgccgagcag gacaaccgta gcgagaatac tcaaaatcat cataacgaaa
     9361 agccccttac ttgtaggagg tctgaccact tgtgatgata tggttgtagt ggatgtaaaa
     9421 acatttagca atgtgtttac aatataatta caacaaagct cacattgttg ctgtttttat
     9481 ccgcacttca ggtcaaaaag tcctggtcat agcacctgcc cgtacttctc gcttttggcg
     9541 gtatccggta cactgcattt tgtctattac atttatgctg aaggatatcc tcatgtacca
     9601 ggatcttatt cgtaacgaac tgaacgaagc ggcggaaacg ctggctaact ttttaaaaga
     9661 tgacgccaat attcacgcca ttcagcgcgc ggcggtcctg ttagcagaca gctttaaagc
     9721 cggtggcaaa gtgctttcct gcggcaacgg cggttcccat tgcgacgcta tgcactttgc
     9781 cgaagagttg accggtcgct accgtgaaaa ccgtccgggc tacccggcga ttgctatttc
     9841 tgacgttagt catatttcct gcgtcggtaa tgatttcggt ttcaatgata ttttctcccg
     9901 ctacgttgaa gcggtaggtc gcgaaggcga tgtactgctg gggatctcca cctccggtaa
     9961 ctctgcaaac gtgatcaaag cgatcgcagc ggcgcgtgag aaggggatga aagtgatcac
    10021 cctgaccggt aaagacggcg gtaaaatggc tggcacggcg gatatcgaaa ttcgcgttcc
    10081 gcactttggt tatgccgacc gcattcagga gattcacatt aaagtgatcc atatcctgat
    10141 ccagttaatt gaaaaagaga tggttaagta agtctggcgt aggccggata aggcgtttac
    10201 gccgcatccg gcacttgtgc gctgatgcct gatgcgacgc tgatgcgtct tatcatgcct
    10261 acaaatctgt acgcgaaccg taggccgaat aagacgttta cgccgcatcc ggcctgaaaa
    10321 ttcttaaatc aatcttcgcc gggggccatg cgctcccgct gttgtggagg ttacccatgt
    10381 gcgaattgct cgggatgagc gccaacgtcc ctaccgatat ctgctttagt ttcaccggtc
    10441 tggtgcagcg tggtggtgga accgggccac ataaagatgg ctggggcatt accttttacg
    10501 aaggtaaagg ctgtcgcaca tttaaagatc cacaacccag ctttaattcc cccatcgcca
    10561 aacttgtcca ggactacccg ataaaatcct gttcggtggt ggctcatatt cgccaggcca
    10621 atcggggcga ggtggcgctg gaaaatactc acccgtttac ccgtgagtta tgggggcgta
    10681 actggactta tgcccataac ggacaactga cgggctacaa atcactggaa accggcaact
    10741 tccgcccggt cggtgaaacc gatagcgaaa aagccttctg ctggctcctg cataaattaa
    10801 cgcagcgtta cccgcgcaca ccgggcaaca tggcggcagt gtttaaatat atcgcctcac
    10861 tggcggatga actgcggcag aagggcgttt tcaacatgct gctttctgac gggcgctatg
    10921 tgatggcgta ttgctcgact aatttacact ggatcacccg ccgcgcgccg tttggcgtgg
    10981 caacgctgct ggatcaggat gtggaaatcg acttcagctc gcagaccaca ccgaatgatg
    11041 tggtcacggt gattgcaaca cagccgctga cgggcaatga aacctggcaa aagattatgc
    11101 caggcgaatg gcgcttattt tgcctcgggg agcgtgtagt ttgatgccag ttgtggctgc
    11161 acaacttcgt ggcttaacgg cttgctgacc acgtaacgac cattgaccac agaaacggtc
    11221 ggtggcttac gggtttgctc aaagtagtcg taacccggct tcagttgctc ccagaagtcc
    11281 ttaaagttgg aatatttatg gcgcttcata ttggcgtcgg tcatgcggaa cgggtaaata
    11341 ctcacttgca cgctcggctg accaaacacc agcgcaccag taacgaactg gaagatctca
    11401 tcaataccct gattggtcat tgcgtagcag ccgatggaaa cacaatcgcc gtggatcatc
    11461 aggtatttcc cttcataacc atgcgcacgg tcataggcat tggggaaacc aatattaatc
    11521 gctttgtagt aacggctgtc tggttttaac tgattacgct ggacgctata aaacccttcc
    11581 gggcttttga aatcgccctg acgctgtttt ggccctaagc cgccggaata tttacagatt
    11641 ttataactgt cgagcagctg atattgctcg cccattttga cgtagagatc gagcgtacgt
    11701 tcttccttga agatctggat gtagacaggg gatcccatca actgctgctt atactctttg
    11761 ctcactggcg tggtcgagct actgctgccc agcaaaccgg caaacgaaac gcacgggatc
    11821 aacaacatcg caagaattaa tgcgatttta cgcatactgc ttattccttg ataaaacggt
    11881 tacacacgcc aggacggcaa aatagatccc aaatcggaat agtctggatt tggaaggctc
    11941 acattatcac caaaagagtt ttacgcaagc ctgtcggcgc agggtttaca aatttcatcg
    12001 gcagcgtgct ttacccaaag agcgccgcgt gagttccagt tctctcaaat cgtaaaagtt
    12061 tatcggtaag tttgtaaatc aggatccagt ccggttcgac atgagcatcg cgataacctt
    12121 tccatgaacc ttgcagcggg tggtctttat aaacagctgg aagcggtaaa gcattattga
    12181 taagaagcgt cataagatat ttcaatttat tcatatcctt atgacgcttt tgtgcaagtt
    12241 ttacatcctt tgaaaattgt cccgagtatt caatatccct ttgaatcata tttaaattcc
    12301 taatttatca aataaatcat cagcgtcttt ggccttatga acatcaacgc cagcttcgct
    12361 gtttttgatt gattgaatgg ttaattgatt aggctcgcgt aaatcaaacg gcaatgcctt
    12421 ttcacgcgcg acctttgtga gggttatgcg aaccaggtca gagatggtca gccccatccc
    12481 ggccagtacg tccgccgcct gattcttcag atcttcatcg attcgggcgc gaacaaacgc
    12541 gttagcagcc attgggggat ctcctgtgct gtatatttat tcagcttgaa ttgtagctca
    12601 aatgagcaac agcgacaatc catgagtaat ttaataaacg gatgttttta cataataaac
    12661 tgaaatatat ttattaattt tataataatg gtttattaag tttgttcagg tacgggccag
    12721 gatacactgc aacatcaatg catgaccagc cattattgag aatgtctttg ccgtcgattc
    12781 cctcttttgt gttgtcggga ttactgttgc tttgcttgcc gttttcttca tttgcctgcg
    12841 ccaccacatc acatatctct ttcagctacg ccgcccgcca gcggatgcaa aaccgtgcgc
    12901 gtttattaaa acagtaccaa acccatctga aaaaacaggc cagctacatt gtggaaggca
    12961 atgcccaaag cagaagggcg ctgcgccagc acaaccggga gcagataaaa cagcatccag
    13021 aatggtttcc tgcgccgctc aaggcgagtg acagacgctg gcaggcgctg gcggaaaaca
    13081 accacttttt aagcagcgat cacctgcata acatcaccga agtggcgatt caccgtctgg
    13141 agcagcagct tggcaagcct tacgtctggg gcggcacgcg gcccgatcaa ggcttcgatt
    13201 gtagtgggtt agttttttat gcctacaaca agatcctcga ggcgaagctc ccgcgtacgg
    13261 ccaatgagat gtatcactac cgtcgggcaa ccattgtggc gaataacgac ctgcgccggg
    13321 gagatttgct gtttttccat atccacagcc gcgagattgc cgaccatatg ggggtgtatc
    13381 tgggcgacgg tcagtttatt gagtcaccgc gcaccgggga aaccattcgg gtaagtcggt
    13441 tagctgaacc tttctggcag gatcattttt tgggcgcgcg gcggatattg acggaagaga
    13501 cgattttgta ggacggataa ggcgtttacg ccgcatccgg cggtggtgcg cagatgcctg
    13561 atgcgacgct gacgcgtctt atcatgccta caaatctgtg cgtttttgcg agccgctttc
    13621 ctgttattcc atcacccagg gaaagatcat cagaagtaca ctttttgtac taaataattc
    13681 gcattttatg tttaaaattt gagatattcc tcattacttg aaactgtttt ttaatgctta
    13741 tgcatgatca aatactcctt acataattaa ggagaacaaa
//
LOCUS       DAETQN010000118        13759 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.118, whole genome shotgun sequence.
ACCESSION   DAETQN010000118 DAETQN010000000
VERSION     DAETQN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13759
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.118"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            254..2020
                     /gene="adeD"
                     /locus_tag="K0W68_003398"
     CDS             254..2020
                     /gene="adeD"
                     /locus_tag="K0W68_003398"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBI3074864.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="K0W68_003399"
     CDS             complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="K0W68_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBI3074865.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="K0W68_003400"
     CDS             complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="K0W68_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBI3074866.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="K0W68_003401"
     CDS             complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="K0W68_003401"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBI3074867.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="K0W68_003402"
     CDS             complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="K0W68_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBI3074868.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="K0W68_003403"
     CDS             complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="K0W68_003403"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBI3074869.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="K0W68_003404"
     CDS             complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="K0W68_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBI3074870.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="K0W68_003405"
     CDS             complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="K0W68_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBI3074871.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9841..9930
                     /gene="tisB"
                     /locus_tag="K0W68_003406"
     CDS             9841..9930
                     /gene="tisB"
                     /locus_tag="K0W68_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBI3074872.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10210..11394
                     /gene="emrD"
                     /locus_tag="K0W68_003407"
     CDS             10210..11394
                     /gene="emrD"
                     /locus_tag="K0W68_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBI3074873.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11402..11899)
                     /locus_tag="K0W68_003408"
     CDS             complement(11402..11899)
                     /locus_tag="K0W68_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBI3074874.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11896..12258)
                     /locus_tag="K0W68_003409"
     CDS             complement(11896..12258)
                     /locus_tag="K0W68_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBI3074875.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12248..12595)
                     /locus_tag="K0W68_003410"
     CDS             complement(12248..12595)
                     /locus_tag="K0W68_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HBI3074876.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12704..13153
                     /locus_tag="K0W68_003411"
     CDS             12704..13153
                     /locus_tag="K0W68_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074877.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13402..>13759)
                     /locus_tag="K0W68_003412"
     CDS             complement(13402..>13759)
                     /locus_tag="K0W68_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3074878.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPA
                     YTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRSLKQEPTEMIQATA"
BASE COUNT         3267 a         3816 c         3570 g         3106 t
ORIGIN      
        1 tcccatgctg aggtagttta aataatcgcg aaagcgtccc tgattcatta ctcacgtaat
       61 cggtattgtc attattcatt tttttgtcca ttggaggaga tttaatccct tgcttttgta
      121 aaacaaatga caacaacggt tcagtgataa ttatcacatt tcaattgcac attaatggat
      181 attctttaat aatctcgcga cgtttcttta tgataaataa taatcaaatt gataaaatca
      241 aaatgagaaa aatatgaata attctattaa ccataaattt catcacatta gccgggctga
      301 ataccaggaa ttgttagccg tttcccgtgg cgacgctgtt gccgattata ttattgataa
      361 tgtctctatt ctcgacctga tcaatggcgg agaaatttcc ggcccaattg tgattaaagg
      421 acgttacatt gccggtgttg gcgcagaata cgctgatgct ccggctttgc agcggattga
      481 tgctcgcggc gcaacggcgg tgccagggtt tattgatgct cacctgcata ttgaatccag
      541 catgatgacg ccggtcactt ttgaaaccgc taccctgccg cgcggcctga cgaccgttat
      601 ttgcgaccct catgaaatcg tcaacgtgat gggagaagcc ggattcgcct ggtttgcccg
      661 ctgtgccgaa caggcaaggc aaaaccagta cttacaggtc agctcttgcg tacccgccct
      721 ggaaggctgc gatgttaacg gtgccagttt tacccttgaa cagatgctcg cctggcggga
      781 ccatccgcag gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca
      841 gaatgcgctg ctcgataaac tggatgcatt tcgccacctg acgctggacg gtcactgccc
      901 gggtttgggg ggtaaagaac ttaacgccta tattactgcg ggtattgaaa actgccacga
      961 aagttatcag ctggaagaag gacgccggaa attacaactc ggcatgtcgt tgatgatccg
     1021 cgaagggtcc gctgcccgca atctcaacgc gctggcaccg ttgatcaacg aatttaacag
     1081 cccgcaatgc atgctctgta ccgatgaccg taacccgtgg gagatcgccc atgaaggaca
     1141 catcgatgcc ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata
     1201 tcgcgtcgcc agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc
     1261 acccggcaag caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca
     1321 ggtactggtg aaaggcgagc cgattgatgc gcaaacctta caggcggaag agtcggcgag
     1381 actggcacaa tccgctccgc catatggcaa caccattgcc cgccagccag tttccgccag
     1441 cgactttgcc ctgcaattta cgcccggaaa acgctatcgg gtcattgacg tcatccataa
     1501 cgaattgatt acgcactccc actccagcgt ctacagcgaa aatggttttg atcgcgatga
     1561 tgtgtgcttt attgccgtac ttgagcgtta cgggcaacgg ctggctccgg cttgtggttt
     1621 gcttggcggc tttggactga atgaaggtgc gctggctgcg acggtcagcc atgacagcca
     1681 taatattgtg gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat
     1741 tcaggatggc ggcgggctgt gcgtggtacg taacggccag gtccaaagtc atctgccgtt
     1801 gcccattgcc ggcctgatga gcaccgacac ggcgcagtcg ctggcggaac aaattgacgc
     1861 cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat gagccgttta ttcagatggc
     1921 gtttctttct ctgccagtga tccccgcgct aaaactaacc agtcaggggc tatttgatgt
     1981 cgagaagttt gccttcacta cgctggaagt cacggaataa taaaaaagcc cggcgtcatg
     2041 ccgggcaaaa gtcaccagtt acgtttatgc cactgtcaac tgctgaattt ttttctcgcg
     2101 gcggattttg cgttcttcca tcaccgccac tatcgccatc aggcagatac aaccaatcgc
     2161 ggcgatatca agtgcggcga aggtgcctgc ccagccggta aggccgaata ccggcgtccc
     2221 atcggcaatc attcccagac ctaacttggc aaagctgtca ccaatcaggt aagcaaaggt
     2281 gcctttaata ccatcggcag cgccaatcgc ttttttaggt acaaagccaa cagcagccac
     2341 accaatcaac aattgcgggc caaagaccag gaaacccaac gcaaagagag aagccagata
     2401 gatatattcg ttactggcat gttgatacac accgagcgtg gcgataatca gcgccagcgc
     2461 gatgcaggcc accaggccac ggcgaccgtt cgccaggtca gagagccagc cccacagcag
     2521 cgtaccgacc agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca ccgctttaga
     2581 gagtttcagt tcctggaacg cgtatacggt tgaccactgg tcgataccaa tacgtaccac
     2641 atagaggaaa atgttggcga agcacagcag ccagatcact ttgtttttca gcacatactc
     2701 aacaaagatc tgccacttgg tcatatcggt agattctgtc tctttgtcct cttcgctgat
     2761 ctcctcgccg aacagttctt cagctttgcc gaggccataa gattccgggg agtcgctgcc
     2821 gtaacgcagg ccgataaaac cgacaatcag cgcgataatc gacgggaaga taaacatgcc
     2881 gatgacatgg ccatcgaaca ggtaatttgc cccgaacagc gccacgcctg ctgcgcctgc
     2941 accgccaagg ttgtgagaaa tattccagaa accgaggaat gtcccgcgtt tacgacgcgg
     3001 cgtccatttg gtgatggtgg agtaactgca cgaaccgccg gtactctgga aaaagccgct
     3061 taaggcgtag aaggcaatca tcaggaacag gctaaccgag ccgctgccca tactggcact
     3121 gaagcccagc atacaaatgg cagacaggat cagcatgaac ggcaggaatt gtttggtgtt
     3181 tttgccgtcg gcgtagtagg aaaccagcgt tttacccacg ccataagtga tggagaaacc
     3241 caggccgatc atccccagct gcgtcatgct caacccgtag gtcgagatca tgtcgttctg
     3301 cgcgatgtta aagttcttgc gaatcaggta catcgtcagg tagccgataa agaccaccag
     3361 gtaggattgc atgaacggtt tgaaccacat tttgcgccgc acttcgagcg gaaggtccag
     3421 ggtcggcttg cgaacctggt ttaagaaagc cagcatgggt tactcctgaa atgaatacct
     3481 gcgtgaggca tgcattgtaa aaatcagcag caaatcaggc ctgagatagc gtccaggcaa
     3541 aacctggaaa atttcttagt tttgccggat atgaagtgaa aaggtgagat gcatcacgct
     3601 tcgcgcggtg tctgggcgtt caaaaagggc agtaacagca gtgcggaaat cccggcggcg
     3661 atagagataa ccacaaaaaa tccgctccag tgccaggtat cgagtacttt cgccagcggc
     3721 caaccagcaa gcgacgcccc cagataagca aacaagccga caaaccccgt cgccgcccct
     3781 gccgcctctt tgtgggaaca ctctgccgcc gccataccga ttaacatctg tgggccaaag
     3841 acaaaaaaac caatggtgaa gaagcaggtt gcctgcatca cgtagctggc aaatggcatc
     3901 agccacaggg agccgactga aagcaaaatt ccggcggcga aaatcaaatt catcggccct
     3961 cggttgccgt taaacaattt gtccgagccc caacccgcta ccagcgcacc gataaatcca
     4021 cccagttcaa acatcgtcac tgccgtattc gccgtgacca gatcgacgcc cagcgtctcg
     4081 gacatataca aattgcccca gtcgttgatc gccgcccgga ccacatagac cagcacatag
     4141 caaaacgaaa gcagccagat atacggattc agcaacacat atttggtgag gatctcttta
     4201 cgcgtcagcc ctgccccttc ttgttgttga gctatctcca gcgcgtcgtg tcgccattca
     4261 ccgaccgccg gtaaacctaa cgcctgcggg cgatcgcgca gccgccagca gagaaaaatt
     4321 cccacgacga tcgccataca accagcaatc atcatcccgg cacgccagcc gtaatgtagt
     4381 gcagccgctg ccatcacaat gggaatgagt gcgccgccga cgttatgcgc cgtgttccat
     4441 aatgcccacc aaccaccgcg ctcggtacgt gaataccagg ccgttaacag acgcgcacac
     4501 accggtgaac cccagccctg gaaaaaggcg ttcagcaccc agagtacggc aaacgcccat
     4561 agcgacgtcg agaagccaaa cagaatgttg ataatgcccg tggcgataag ccctatcccc
     4621 ataaaataac gggcatttga gcgatcgctg acaatgccgg agacaaactt cgacacgcca
     4681 taggtaatgt aaaacagggt cgctaacagg ccgatatcgc tacggctgag cacgccgtta
     4741 gcaaggattt ctggtacggc ggcgttaaaa cttttccgcg tgaagtaaaa cagcgcgtaa
     4801 cccagccaga tggtcagcag aatatgccga cgccagtagc gatagcgggc atcaatttca
     4861 tatttatcag ccattaatgg cgcatcggca ggcgctttca gaaacggcaa catcatcaaa
     4921 ccttagacat agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg
     4981 tgtaatgtgc cacccagcgc cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt
     5041 tgcccggaac ccggcggcaa accgctgcca tcgtcttcaa taaccagcat caaccgttca
     5101 tcctgctgcc agccttgcag ggtgaccgcg ctggcatcag catgtttcac aatgttgttc
     5161 agcccttcct ggcagacacg aaacagcgtc acgcgctggt tttcgcttaa cgctgattca
     5221 tcgattcgcc attcgagatg gctgacaata ccgcgccctt ccagctccat ttcccgcatc
     5281 agtgagcgga tggcctgctc cagggtgaga tcatccaact ggcgcggacg taaccgaccc
     5341 aacaaacggc gcaccgcgtc gtaaacgccc agcgatagtt gttcgatgag ttgcccgctc
     5401 tgcttcacgc tggcgttatc tgccgccagc cgctgaacaa tgcccgcctg agtacgaata
     5461 gcagtgatgg tctggccgat atcatcatgc agctcacgcg ccacatcacg gcgcacgctc
     5521 tcttcggttt ccagcaaccg ttcagccaga tgctgattgc gcgccagttc cttttgcagc
     5581 gactggttaa gttcacgcaa ccgctggatg ccagcgccaa gcaacaaccc tgtcagactt
     5641 tgcaccagca gcgagagcaa taaatccacc ggatgatcgc gccaggtttg actggcgatc
     5701 agcgcgatgg cgttcatcaa cgtcgcaatc agcgcccctt gccaaccata gtgccaggcc
     5761 agcgcgataa tcggcagcac cagacagaat ggcgtaaagc gcgacagttc gtccggcaat
     5821 cccaactgga gccagagact gataacaaac agcagcaagt accagaccag atgtcgcccg
     5881 cgccagttga ttggctgaga aaccagtgac gggccgagcg gcagccaggt gttattggcg
     5941 agatagtgcc agaacaccag acatatcggg gccagcgtca ggccgccagt taaagtcagc
     6001 aacagcgcat tccacgactc tttgccgtgc caaagccagg gcagcgactg caacaacgcc
     6061 gccgccgtta acgccgcccc ctgtaacagc aaggtgcgcc agtcacgctg atggcgatag
     6121 cgcgagatca gcgctacggg cagtaacgtc agtaaactac cgatcatcaa taacggaaaa
     6181 tgggttaaac cgaccgcctg cgttagccag taaatcagca gccactccgc acccagcaat
     6241 acgggccagt atccgcgcgg gcattgcagc attagcccca gacgcagacc aaacggaaat
     6301 aacagcaccg ccatatcagg gcgctcaacc agatgcaggc tgatactcca caggcaaaac
     6361 catgcggcag agaagataaa aaagcaggca ataacggtaa ttaagcggga gaacaacgtc
     6421 ttcatcacca gccatcaaac atgcggcgcg ccagctctac gtcgttactg acgcccagtt
     6481 tttccatcag attggcgcga tggacgtgta ccgttttcgg tgacaagccc agttcggcgg
     6541 caatctcttt caccgccatt ccttgcgcca gtttttccgc cacctggcgt tcacgtttgg
     6601 ttagcgggtc ctgacgaccg gatgccagtt taatggcaat atccggcgtc agataacagc
     6661 cacccgtggc aaccgtatgc accgcagcaa tgagttcatc cgggctacag cgtttggaaa
     6721 gaaagccgcg tgcccccgcg ttaagcgcct gctcaaccag cgcaggactg tcgtgaacgg
     6781 agagcataat cgtcgccata cctttcggca gctggcttag cagctccaga ccggagatat
     6841 cgggcatgga gatatcgcaa atacacacct gcacaccgcg ccccggcagc cccgccagcg
     6901 cctcgcgccc cgaaccaaac tcggcaacta cctgcaaatc aggttccagc cccagcagct
     6961 gcgcaaagcc ggagcggacg atgaggtgat cgtctataag ggcaacggtg atcatggtct
     7021 tgtcctggcg ggtaaaaaaa aacgcgctta ccttaacgat aagcgcgatg ttgttcaagc
     7081 cttgagcggt tactgaaaaa acaccgcgat cttgttaaac atcgtcggat cggactgatt
     7141 acgctgcact ttcacgacat cttccagctt atcgatttgg cttatcatct gctccagacg
     7201 ctggtcgtca ttgaccagta gccagatatg gcttttgtcg ctgtcctgaa tcggcagaca
     7261 aagaatgcct tcaacgttaa aagcgcggcg ggcaaaaagg ccacaaacgt gggtcattac
     7321 gcccggatgg ttgcgaacgg tgagctccag aattacgttg tcatgagttg tgttttgcat
     7381 ggcttattcc cccaccattt cagtattcgc cgcacctggc ggcaccatcg ggtaaacttt
     7441 ttcttcggca tcaatgcgca catggatcag cgccgggcta gggcgattga tgatttcctg
     7501 caatgcagcc tgcggatcgg cttcgttatt caaatcacag gtttcgaggc cgaatccggc
     7561 ggcaatctgc ataaagttga ttttgcccgg ataggtggcg gcaaaaacgc cttgctcgta
     7621 gaacagactc tgttgctgat gcaccagccc cagcgcttcg ttgttcatca gaatgatttt
     7681 gacatccagc tgattttcac tggcggtcgc catctcctga atattcatca tcaggctgcc
     7741 gtcgccggag aaacacaaca ctttgcgatc cgggttcgcc agcgccgcgc caatcgccgc
     7801 aggcaggcca aaacccatcg tgcccagccc accggaggtc agccactggc gtgggcgatt
     7861 gagcggataa gcttgcgcgg tccacatctg atgctgaccc acatcggtgg tgataatcgc
     7921 attgtcatcg acacaggcgg caacggcgtt gatcaggccg taatggctta atggatcgca
     7981 cgctttcggg attggacacg ggaattcgcg ctgcaaatcc gctaccaact ggtgccactc
     8041 tgcacgcggt tgcgcttcca cctgcgggat caattgcgcc agcacgtcat caacatccgc
     8101 ctgaatcgcc acatgcggct gcttgatttt acccagctct gcgcgatcga tatcgacatg
     8161 aataattttg gcattcggac agaactgctc ggttttgcca atcgcccggt catcaaaacg
     8221 cgcaccgagc acaatcaaca aatccgcctc ctgcaaaata tagttggtac tgcgcacgcc
     8281 gtgcatcccc agcataccca gcgacaacgg atgcgctttt ggcagcatgc ccagcgccat
     8341 taaagtcatg gtggtaggca gttgcgcttt ctccgccagt tcacgcaccc gcgcaggcgc
     8401 attgatcaca ccaccaccca gataaagcac cgggcgtttg gcagcgttaa tcattgtagc
     8461 tacgtcacga atgctttctt cgctaaaggc gggtgcagcg gctttttctg ccaccgcggg
     8521 ctgagcttca atctcaaaaa ccgccgtttg tacatcctta ggaatgtcta tccacaccgg
     8581 gccagggcgg cctgattgcg caatgcggaa ggcatcgctc atgacctgcg ggagttcttc
     8641 gatatgtctg accagatagt tgtgtttggt gatggggata gagatgccgt aggtgtcgac
     8701 ttcctggaag gcgtcggtgc cgatcatcga ggcgggaacc tgaccagtga tgcaaatcag
     8761 cgggatggag tccagccgcg catcggcaat ggcggtcacc aggttagtcg cacccggtcc
     8821 gctacaggcc atacagaccg ccggtttacc gtcggtgcgc gccattccct gagcgataaa
     8881 gcccgcgccc tgttcatgac gggccagaat atggcggatt tgcgtgcttt ggcttaaggc
     8941 atcgtaaaca ggcaggatag aaccgcccgg aatgcctgtc acaatcttaa tgccctgctg
     9001 ttccaggaaa tgaacgataa attctgcgcc ggtaaagcgc ttacgcgtca atgttgtgcc
     9061 cgaacttgcc atgctccagt ccttttcttc tgggccgact ttccgggagg tgcttaaacg
     9121 aaaaaccccg cccggtttgc gccgacgggg ttttggaatc gtgtgttgtt ccagtcccta
     9181 cggcgcattg ccgacgacca ccaccacacg cacgacgacc actgcggcgg atggcgcagt
     9241 tggtagtagt tttgcgttga acatggaagt agtcattggg gaccttgttg gttttgtgtt
     9301 taacaatatt tatacaagca cagctttaca ggggagacaa tggaaaattt ttcagcaagg
     9361 gaaaattgag gggttgatca cgttttgtac tgaattgcag ataacaaaaa accccgccgg
     9421 agcgaggttt cgtcagtcgc ccgcggctgg taaccgcaaa gcacactgta ttatgtcaac
     9481 acagaaagta tacgtgttcc gcgcagaacg cgcaatttcg gcacgaattt tgacgtattt
     9541 agtgcatagt tgagtatcga tcacagtttg cgttttgtcc aaatattgct gtttatttat
     9601 acagtaaact tctataatat cactgtacgc aatgtgttat gcgggggccg catcgttacc
     9661 cggcgcacta agtcctggct gaaacgggtg gtgccgtcag cgccttaacc ccgcgtgagc
     9721 acactgtgtt atgtcaacaa gcacaaagtt tctccttgag ataccgcgtg cacaacagct
     9781 ggcaacaggc agcggaaagg tacgtcagct ggcagtgctc ctgaaccaca ggagacgcgt
     9841 atgagcctgg tggatatcgc cattcttatc ctcaaactca ttgttgcagc actgcaactg
     9901 cttgatgctg ttctgaaata cctgaagtaa ttcagattca agtcgcacca aaggggagcg
     9961 ggtaaccgct ccccttttat atttagcgtg cgggttggtg tcggatgcga tgctgacgta
    10021 tcttatccgc cctaccatct ctcccggcaa catttattgc cgcttttgtt tacatattct
    10081 gccgctaaac aactccccat tcctggcgta tatctggcta acattcatca atgtgataga
    10141 ttcctctccc gcatttatgg gaatgcgtag tgacttactc taattatttt tataaaagca
    10201 tccgtgataa tgaaaaggca tagaaacgtc aatttgttat tgatgttggt attactcgtg
    10261 gccgtcggtc agatggcgca aaccatttat attccagcta ttgccgatat ggcgcgcgat
    10321 ctcaacgtcc gtgaaggggc ggtgcagagc gtaatgggcg cttatctgct gacttacggt
    10381 gtctcacagc tgttttatgg cccgatttcc gaccgtgtgg gtcgccgacc ggtgatcctc
    10441 gtcggaatgt ccatttttat gctggcaacg ctggtcgcgg tcacgacctc cagtttgacg
    10501 gtattgattg ccgccagcgc gatgcagggg atgggcaccg gcgttggcgg cgtaatggcg
    10561 cgtactttgc cgcgagattt atatgaacgg acacagttgc gccatgctaa cagcctgtta
    10621 aacatgggga ttctcgtcag tccgttgctc gcaccgctaa tcggcggtct gctggatacg
    10681 atgtggaact ggcgcgcctg ttatctcttt ttgttggttc tttgtgctgg tgtgaccttc
    10741 agtatggccc gctggatgcc ggaaacgcgt ccggtcgatg caccgcgcac gcgcctgctt
    10801 accagttata aaacgctttt cggtaacagc ggttttaact gttatttgct gatgctgatt
    10861 ggcggtctgg ccgggattgc cgcctttgaa gcctgctccg gcgtgctgat gggcgcggtg
    10921 ttagggctga gcagtatgac ggtcagtatt ttgtttattc tgccgattcc agcagcgttt
    10981 tttggcgcat ggtttgccgg acgtcccaat aaacgcttct ccacgttaat gtggcagtcg
    11041 gttatctgct gcctgctggc tggcttgctg atgtggatcc ccgactggtt tggcgtgatg
    11101 aatgtctgga cgctgctcgt tcccgccgcg ctgttctttt tcggtgccgg gatgctgttt
    11161 cctctggcga ccagcggcgc gatggagccg ttccccttcc tggcgggcac ggctggcgcg
    11221 ctggtcggcg gtctgcaaaa cattggttcc ggcgtgctgg cgtcgctctc tgcgatgttg
    11281 ccgcaaaccg gtcagggcag cctagggttg ttgatgacct taatgggatt gttgatcgtg
    11341 ctgtgctggc taccgctggc gacgcggatg tcgcatcagg ggcagcccgt ttaagcgcac
    11401 gtcaccgcag catcgtcatc agctccatgg gagaacgatg ctgctttatc agatcacgca
    11461 tcacccgcat atgcggtgcg gagtaagaat aaaacgcctg atagcccgca caaagcacgc
    11521 tgtctggcgt ctcctgccga gcgtgcgggc acaggcgatg ccagtcgcac gcctggcagt
    11581 cacagcgcgg ttgcgggttt tcgctgccac accatttttg taaggtggcg tcaaaaagct
    11641 caatcgagat acgactgata tctgcccgca cccaacactc gaacagggca attaaaaatt
    11701 tcccccacag ctctgctgtc accgaagcgg ccgtcagatc gccccggtca tcaaattcaa
    11761 gatagggaat gagctgtacg gagcggatgc cccgctgttt gaaattgtga taaaaatcgg
    11821 caggttgcag agcatccttg tactctacaa ccagtttatg cctgtcctct tcagcatcaa
    11881 tgacctgact tcctgtcatg cgactctctc aataaatacg ataagttgat gtatatgcgt
    11941 tacagcaaac agtattgcga gagataacac cgcgagggag atcaaaaatt tgtcacggac
    12001 tacgtgaaat tgggaaaaat cgctattcgt gacatccatt aaattgcgat tacgggtgta
    12061 gtgccagagg atcagcgcca cgatggcgag aatgccaatc gaaatccaga ataacatgcc
    12121 cgcctggtgc cagttgtgtt tgatagccaa cgccatcaac gcgccgtagc ccagcatggt
    12181 acgaaaccac gccagtgatg tgcgctccgg ctgcagtcct gggtcggcaa tgcgtctggc
    12241 tttgcggcta tccggcatac aacaccagtc ccataacgat caccgccacg accatcaata
    12301 ttaagctaat gattaataag ctgttggtgt agggcaggtc ctctttcaga cgcatcgctt
    12361 tttcattgcg taaccagcgc aggtagccat acattgctaa cccgccggaa aacagacaca
    12421 acaacaacgc cagcagttca cgaatgacgg gcgtggcgaa atctggcgca agttgatcaa
    12481 gcccaacccc cgccgccaga aatcctagtg cggtacggat ccacgccaga aaagtacgct
    12541 cattagccag cgagaagcgg taatcgggcg cttctccaag gcgggaaatt ttcattgttg
    12601 ctcctttgca atttcactaa ctctctacct ttagcggttt tttttaagaa tttcgtccgc
    12661 ccgtttcctg atatgctttt gccatccgct acaaggaggt cagatgggta tcatagcgca
    12721 aaataaaatc agttcattgg gcatgctgtt cggtgccatc gctttaatga tggggattat
    12781 ccacttttca ttcggccctt tttccgcacc accgccaacg cttgaaagca tcgtggcgga
    12841 caaaacggct gaaattaaaa gaggtcttct cgccggaata aaaggcgaaa aaattaccac
    12901 cgtggagaaa aaagaggacg tagatgtcga taaaatcctc gatcaaagcg gtatcgcact
    12961 ggccattgct gcgctacttt gcgcatttgt tggcggtatg cgtaaggaaa atcgctgggg
    13021 cattcgcggc gcgctggttt ttggcggcgg aacgctggct ttccatacct tgctttttgg
    13081 tatcggcatc gtgtgcagca ttttgctgat attccttatc ttttccttcc tgaccggcgg
    13141 ttcgctggtt taagcagcct gaataatgcc cgccgggtga tcatcagcgg gcattatcgt
    13201 cagaacttct gttttttctc ttccaccttc accccttgtg tcggtaggcc ggtgtcatag
    13261 tcacgtacca ccggcgaata gccatcttgt gggcgtggac gaaatgcccc catccagcgc
    13321 ggccgtgcat ctttacgcca cggacgcagg ctccattgtt gacatcctcc acgccctgaa
    13381 ggacgtggac ccctgctacg ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt
    13441 caacgagcgg cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa
    13501 aatgttacga gcgccattta catcggcgtt cgctgtatat ccacatacct ggcatctgaa
    13561 tttactttgt gacaggcgat tttctttcgc tgtatgacca cagcacacgc aacgctggct
    13621 tgtgtacgct ggcggaacag caagcacctg accaccacgc cagagctgct tgtacgcaag
    13681 ctggcggcgc atttcatacc agccctgatc cagtatcgaa cggtttaaac ctgattttgc
    13741 ccggacattg cgccccggc
//
LOCUS       DAETQN010000119        13260 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.119, whole genome shotgun sequence.
ACCESSION   DAETQN010000119 DAETQN010000000
VERSION     DAETQN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.119"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="K0W68_003413"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="K0W68_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBI3074879.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="K0W68_003414"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="K0W68_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBI3074880.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="K0W68_003415"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="K0W68_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBI3074881.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="K0W68_003416"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="K0W68_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBI3074882.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="K0W68_003417"
     CDS             complement(4036..5568)
                     /locus_tag="K0W68_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBI3074883.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACHGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="K0W68_003418"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="K0W68_003418"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBI3074884.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="K0W68_003419"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="K0W68_003419"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBI3074885.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="K0W68_003420"
     CDS             complement(8663..10399)
                     /locus_tag="K0W68_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBI3074886.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="K0W68_003421"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="K0W68_003421"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBI3074887.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="K0W68_003422"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="K0W68_003422"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBI3074888.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="K0W68_003423"
     CDS             complement(12121..12855)
                     /locus_tag="K0W68_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBI3074889.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13260
                     /locus_tag="K0W68_003424"
     CDS             13056..>13260
                     /locus_tag="K0W68_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBI3074890.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3365 a         3370 c         3489 g         3036 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatggcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc tgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
//
LOCUS       DAETQN010000120        13210 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.120, whole genome shotgun sequence.
ACCESSION   DAETQN010000120 DAETQN010000000
VERSION     DAETQN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.120"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..807
                     /locus_tag="K0W68_003425"
     CDS             <1..807
                     /locus_tag="K0W68_003425"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBI3074891.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1177..2001
                     /gene="gadX"
                     /locus_tag="K0W68_003426"
     CDS             1177..2001
                     /gene="gadX"
                     /locus_tag="K0W68_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBI3074892.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            2369..3097
                     /gene="gadW"
                     /locus_tag="K0W68_003427"
     CDS             2369..3097
                     /gene="gadW"
                     /locus_tag="K0W68_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBI3074893.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3460..6573)
                     /locus_tag="K0W68_003428"
     CDS             complement(3460..6573)
                     /locus_tag="K0W68_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBI3074894.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6598..7755)
                     /gene="mdtE"
                     /locus_tag="K0W68_003429"
     CDS             complement(6598..7755)
                     /gene="mdtE"
                     /locus_tag="K0W68_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HBI3074895.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7815..8093
                     /locus_tag="K0W68_003430"
     CDS             7815..8093
                     /locus_tag="K0W68_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074896.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="K0W68_003431"
     CDS             complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="K0W68_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBI3074897.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="K0W68_003432"
     CDS             complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="K0W68_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBI3074898.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            10247..10579
                     /gene="hdeA"
                     /locus_tag="K0W68_003433"
     CDS             10247..10579
                     /gene="hdeA"
                     /locus_tag="K0W68_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBI3074899.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10695..11021
                     /gene="hdeB"
                     /locus_tag="K0W68_003434"
     CDS             10695..11021
                     /gene="hdeB"
                     /locus_tag="K0W68_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBI3074900.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            11085..11732
                     /locus_tag="K0W68_003435"
     CDS             11085..11732
                     /locus_tag="K0W68_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBI3074901.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11774..12304)
                     /locus_tag="K0W68_003436"
     CDS             complement(11774..12304)
                     /locus_tag="K0W68_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3074902.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12460..13026)
                     /gene="slp"
                     /locus_tag="K0W68_003437"
     CDS             complement(12460..13026)
                     /gene="slp"
                     /locus_tag="K0W68_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBI3074903.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3599 a         2982 c         3030 g         3599 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca cacctgataa cataacgttg taaaaaccga atgcccagcc
      841 tttaaaaaaa cagctgggca ttcggttgct tattaatcgc aataatatat tggctgtcta
      901 ttcatcgtgt tgataagata tatgttatat gaatgtttat taaatgtagc gattgccttt
      961 aatgtcacga aaagggatta tttgcttgct attaatttca ctgcgttctt aaacacattt
     1021 ttgtattata aatccagtca tcctgcccgt agaacatcac acattatcat cctgttctcc
     1081 cgcttcgttt aaatttattt atcaatcaat ttgacttaag agggcggcgt gctacattaa
     1141 ttaacagtaa tatgtttatg taatattaag tcaactatgc aatcattaca tgggaattgt
     1201 ctaattgcgt acgcaagaca taaatatatt ctcaccatgg ttaatggtga atatcgctat
     1261 tttaatggcg gtgacctggt ttttgcggat gcaagccaaa ttcgagtaga taagtgtgtt
     1321 gaaaattttg tattagtatc aagggatacg ctttcattat ttctgccgat gctcaaggag
     1381 gaggcattaa atcttcatgc acataaaaaa atttcttcat tactcgttca tcactgtagc
     1441 agagatattc ctgtttttca ggaagttgcg caactatcgc agaataagaa tcttcgctat
     1501 gcagaaatgc tacgtaaaag agcattaatc tttgcgttgt tatctgtttt tcttgaggat
     1561 gagcacttta taccgctgct tttgaacgtt ttgcaaccga acatgcgaac acgagtttgt
     1621 acggttatca ataataatat cgcccatgag tggacactag cccgaatcgc cagcgagctg
     1681 ttgatgagtc caagcctgtt aaagaaaaaa ttgcgcgaag aagagacatc atattcacag
     1741 ttgcttactg agtgtagaat gcaacgtgct ttgcaactta ttgttataca tggcttttca
     1801 attaagcgag tcgcagtgtc ctgtggatat cacagcgtgt cgtatttcat ttacgtcttt
     1861 cgaaattatt atgggatgac gcccacagag tatcaggagc gatcggcgca gggattgccg
     1921 aaccgtgact cggcggcaag tattgttgcg caaggaaatt tttacggcac tgaccgttct
     1981 gcggaaggaa taagattata gaattttact cagacataaa aaaaacccgg cataggggac
     2041 cgggaagagg atagtctgcc gtctccagac taataaaccg ttataacact ccctgttggc
     2101 acgggaaact ttgtgctctc agtaagttaa atataacttt tactggaaat aagatcagcc
     2161 atttttttat aaacataagc tatacgctgt gcgaaaatat attcgtgctg catttactta
     2221 ttatcaatta actgttatgc aaaactactt tgtggataaa ttttggtcct accaaatctg
     2281 gcagtttttt cgctaagaaa cagtctggca acatttcatt agtatactga aattgaaata
     2341 atcgcagtat gaaatataag ggataatcat gactcatgtc tgctcggtga tcctcattcg
     2401 tcgttcattc gatatttatc atgaacagca taaaatatcg ctgcataacg agagtatcgt
     2461 gctgctggag aaaaatttgg cagacgattt tgcgttttgt tcaccggata cgcgacgact
     2521 ggatatcgat gagctgacag tttgccatta cttacaaaat attcgtcagc taccacgcaa
     2581 tttagggtta catagcaaag accgtctatt aattaaccag tcacccccca tgccgctggt
     2641 gacggcgatt tttgatagct tcaatgaatc cggggtaaat tcaccgatac tgagcaatat
     2701 gctctacctt tcctgtttat cgatgttttc tcataagaaa gaactgatcc ccttactttt
     2761 caatagcatc agcactgttt caggaaaagt tgaacgcctt attagctttg atatcgccaa
     2821 acgttggtat ctgcgcgata tcgcggaaag aatgtatacc agcgagagtc tcataaaaaa
     2881 aaagttgcag gatgaaaata cctgtttcag taaaatatta ctcgcttcca ggatgtcgat
     2941 ggccagacga ttactcgagt tacgtcaaat tcctctgcat actattgcag aaaaatgtgg
     3001 ctatagcagt acgtcgtact ttataaacac atttcgacaa tattatggtg taacgccaca
     3061 tcagtttgcg caacattcgc caggtacctt ttcctgacat attttgcatt tgaatattgg
     3121 tcaggatctc acacctgctt catgtgaaac tcttccctga tgatttctgc cgggctaccg
     3181 gctagttctc tttcgcagtt tgccaggcgg tataggtatt ttcaggagga aaagactcgg
     3241 catgtttggg attattaagc tgacaattca tacgattacg ggaatgtggg tcagtattgt
     3301 gctgttcaaa ttgatgacga atggctggag tggtttctac tttcagtgct gtgtactaag
     3361 cctggtattc ctgacggtta gctggttgtt gagtggtgaa tggttagcag gaaagagtaa
     3421 ggctgaacct tcacgttcaa ccttactctc atttacacgt tacgcttttt taaagcgggc
     3481 aaagagatgt tccactacaa cgaaaaagac cggaacgaag taaattgcca gcactgttgc
     3541 ggcaaacatc ccgcccatca cgccggtacc taccgcgttt tgcgcgccag aaccggcacc
     3601 atgactgata accagcggca gcacgccgag aataaaggcg agagaggtca tcaggattgg
     3661 gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc ggcgttttcc cttctttctg
     3721 catcatctca acggcaaatt cgacaatcag gatggcgttc ttggcggaaa gcccgatggt
     3781 ggtcagcaaa ccaacctgga agtagacgtc attacttaag ccgcgcagat cggtggccag
     3841 taatgcgcca acgacgccta acggaacaac caacatcacc gagaacggaa ttgaccagct
     3901 ctcatagagt gcggcgaggg cgaggaacac cacgaccagt gaaatggcat acagtgcagg
     3961 agcctggttt gaggataacg cttcctgata cgacagtccg gtccatgagt agccgacgcc
     4021 tgccggaagt ttagcgacca ggtctgccat aaatttcata gcgtcaccgg tacttttccc
     4081 ggccgccgct tcacctaaaa tctccattga cgggatgccg ttgtagcgtt ccaatcgcgg
     4141 tgaaccatag gtccattcgg tagacgagta ggcagaaagc ggtgccatcg tgccagaggc
     4201 gttgcgtaca taccattggt tgatgttatc cggcaacata cggaacggcg tgcctgcctg
     4261 gacatacact tttttcaccc gcccctggtt gaggaagtcg ttcacgtagc tgctgccgaa
     4321 ggcggtggaa attgtctggt tgatatcaga cagcgccaca cccattgcct cagctttcgc
     4381 ggcgttgacg ttcactttga acatcggcgt atcttccagg ccgttcgggc gtaccccggt
     4441 gacctggtca ggcgattgcg ctgccagtga taacagctcg tttcgtgcct gggttagttt
     4501 ttcgtgcccc aggttaccgt tgtccagcag ttccatatca aaacctgacg cggtacccag
     4561 ttcagccacc gcgggtaagt tgaacgggaa gacgacggct ttattgatac tgcttaacgc
     4621 aatcattgcc cgctgaatga tcgcggtaac cgagttttcc tcaccgacac gttcagacca
     4681 cggcttgaga ctgataaacg ccaggccgtt gttttgcccc tggccgctga agccaaagcc
     4741 gccaacggta aacaccgact gaacattatc tttctctttt gtaagataat aatccgtcac
     4801 ctgttgcagc actttcgtgg tgttaaccat ggtggcaccg gaaggtaact gcgcggtggt
     4861 cataaatacc ccctgatcct cttctggtaa gaaagaggtc ggtgtacgca ggaacagcac
     4921 cgccatcccg gcgcaaatca gcaggtagac caccatgtag cgaccggtac aacgcaacag
     4981 cgagcgggtg ctatcggtat agtgctgagt tgatttttca aacagcgtgt tgaagcgtgc
     5041 gaacagggcg ttaggtttgt gaccgccttc cggcgcggct ttcagaatgg tggcgcacag
     5101 ggcaggggtc aggctcattg ccacaaatac tgaaagcagc atggaggaga tcagcgtgat
     5161 ggagaactgg cggtagatct ccccggttgc gccgctcata aaggccatcg gcataaacac
     5221 tgcggaaaga acaacagcaa taccgaccag cgcacgttgg atctgcccca tcgatttatg
     5281 cgtcgcttcc ttcggtggta gcttatcttc cgcaatgaca cgctcgacgt tctccaccac
     5341 cacgatggcg tcatccacca gtaaccctat cgccagcacc atcccgaaca tagttaacgt
     5401 gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc ccgagaataa ccaccggtac
     5461 ggcaatcgtc gggatgattg tggcacggaa attctgcaaa aacagataca tgaccaggaa
     5521 gacgaggatg atagcctcaa ccagtgtttt aaaaacttcc tgaatagaaa tttcgataaa
     5581 cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcg gggaaatagg ctgataagcg
     5641 gttcagctcc tctttgactg cccgcgaggt atccagggcg tttgctccgg tagccagttt
     5701 gatggcgatc ccggcagcag gtttgccgtt atagcgcgcc acggtggaat aatcttccgc
     5761 tccaagttcg acgcgggcga catcacgcag cagcacttgc gaaccatctt gctgaacttt
     5821 caacaggatt ttgccaaatt cttccggcgt ttgcagacgc gtctgcacaa tgatcgaggc
     5881 gtttagttgc tggtctgccg cctgtggcat gccacccagt tgaccaccgg aaatctggtt
     5941 gttttgcacc ttaatctggg aaataacatc ggaaggtacc agattgtatt tattgagttt
     6001 ttgcgggtcc agccagatac gcatggcgta ctcggaacca aagagctgta cgctaccaac
     6061 gcccgcggta cggcttagcg ggtctttgat attagacgct acatagtccg cgatatcgta
     6121 ctggttgagg ctgccgttat cagaaataaa cgccgctacc atcagaatat tactgctcga
     6181 cttatcaacg ctaatcccct gctgctgcac cgcttcaggt aatgaaggca tagcgagttg
     6241 cagtttattt tgcacctgaa cctgtgcgat atcaggagat gtcccagtct cgaaggtcag
     6301 agtgatagag gcattacccg ccgcatcact ggttgaagac atgtacatca ggccatcaag
     6361 cccattcata ttttgctcaa tcacctgagt gaccgagtct tctaccgttt gcgcatcggc
     6421 acctggatag gtagcgctga cggtaatggt cggtggcgca atctgcggat actgcgcaac
     6481 cggtaagttc atgatcgcca gaccacctgc aagcatcata ataatggcaa gtacccaggc
     6541 aaaaaccggg cgatcaataa aatagttagc catgaaagtc cccttaagcc tgcaacgtta
     6601 ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa attgctcgtg ctttgatacc
     6661 cggacgaatg cgttgcaaac cggaaacgat cacccgatcg ccagcctgca agccagaggt
     6721 gacaacccac tggtcgccga tggctttgct ggcttcaatt tcgcgtagct gcacaacatc
     6781 gtctttatcc agaatgagcg ccgttgcttt accctgggcg ttgtgagtga cgccttcctg
     6841 cggcaccagt aatacattct ggcggctacc ttcatccact aatgccgtga cgtacatgcc
     6901 aggcagcaag tcaccatttg ggttggggaa aatcgcccgt aacgtcacgg agcccgtggt
     6961 ttcatccacc gtcgggtcgg agaatttcag tgtgccggtc tggctgtagc gtttaccatt
     7021 ttccagattg agctgtactg gcgtactgcc ctgaacctgt ttgatttgcc cactggcgac
     7081 ctcttctttc atgcgcaaga aatcctgcac cgactgcgtg agatcgacat aaatcgggtc
     7141 cagacgttgt acggtaacca gcgaatctgc ctgattagcg gtaacgagtg cgccgacggt
     7201 caccgacgat ttcccgctga cgcccgtaat cggcgaggtg acattcgcgt attgcagatt
     7261 gatcgtcgcc tgttcaacag ccgctttggc gacggtgaca ttggcttctg cttcattcag
     7321 ctgggtgcgc gcggtgtcgt aatcctgacg gctaacgtag ttggtcttca gcaacgatgc
     7381 ctggcggtta aaggtgatgc gggcattgct ggcggtagag agcgctttcg ccagcgagcc
     7441 tttggcggag tttagctcgg cctgtaaagg tgcaggatca atctgataca gcgaatcgcc
     7501 ctggttcact ttatcgcctt cgataaagtt gcgtttaatg ataataccgc ccacctgggg
     7561 acgtatctcg gcaacttcat aaggaacggt tctaccgggc aattcgctca acacattgac
     7621 cgaaccgggg gagagtgtga cgacaccgac ctcaggcgtc atggcggcgg cgttttccgc
     7681 cgatttgtca tcgcaggcgg tgagcatcgc gccgcagaat aacaacggta ttaacagctt
     7741 tcttcttctg ttcattttag tccctgaaaa ttcttgagaa ataacggttt tttaaattgc
     7801 gcgcgaggag agatatgagg gatttacaga ccagcggcat agtggggttg tcagccagta
     7861 aggtgggata caggcacagt gatcgacatg gtgaggtcaa cgacaaaaaa aatgttgccg
     7921 ttctgccaac agttcctgcc agcattcggg caacgaaggg ttctactggt ggatacacat
     7981 acctggggaa taaagtcgtt ttagcctctc ctttagtaat caccgggggt aatgaaatca
     8041 gcatctgcat ccctcgtcat gccagccatc aatttcagtt gcttatgtcc tgactaaaaa
     8101 taagatgtga tacccagggt gacgatgtcg ctcatacgtc ttagtttcag cttgtacata
     8161 atgttgaatt tctgaatctt taatgtttgt tgcgaaatat tgagcgtcga cgtgatattg
     8221 ctttcatttt tatttttcag aatcagttgc ataattttga actgattatg tgaaaaacat
     8281 aattgtgaat tcttatgggg caagtgttta ccataagtaa tctcaagaag atttttgata
     8341 ctttctttgc ggctaacaaa ataaataggc gctttagctt ttagtttact gatgtgataa
     8401 ggtgccaaaa caataattct ctcggcatct aatttctcca gaaatttaat cgcttcttca
     8461 tcaaggatat gattgtgata ggtatcaatg ataaccttga agggtttatt gcctactttt
     8521 ttgatctctg acagggaatt gatttttatc atttcgtgat tatctttcaa ctgccaaaag
     8581 ccctgtaaaa gaaaagaatc tttcgtcatg agaaaaatca taacttgctc cttagccgtt
     8641 atcgtttcga acatgtcatc cttttcatta tttacatcct tgtccgaatc gttgttcaat
     8701 atagtaaacg cctataagaa ctttaccctt cgtgaactaa tacgtctatt aacactaatc
     8761 aatacttcag atttatccat cacgcactcc atatattgat cctaagtaag attcctggtt
     8821 gttatcagct tgtaaatagt aaacaaaaag ttaacgtttg cggattactg cccaagaata
     8881 agttatcaat aaccagtttg tgatctctga agaatattac taaagttaaa atgtaatccg
     8941 atttaaatat cgagtctcct tgtttcgact taagctggca attggattgc cagctttctt
     9001 ttaacgcatt gaaattacct gagtaaataa cgcattcatg tcttcaggtt tttatatagc
     9061 gtctggagac acggatattt atgcaatgta atcaacgcca gttgcaaaaa aatgaacccg
     9121 gattacttat ttaaccgccc atagcatcaa cgcttacgga actctttgtt cactttccca
     9181 cagcactttt aaaacattaa acctgactac ggaaaatatc agccatgata atgtttgaaa
     9241 tggctaattt gccatggagt gaaaaaaatt agatgaaatt cagtaggttg aaataatcac
     9301 tagcaggtaa ttatttcaat gatagtgcgc aattgatcta caacactccg tagcggagag
     9361 agtattaatc ggatcatagt catatcaagt gactatgatc cgggtgacaa ccgggataat
     9421 tattgctgct taacgaacaa actggcgaag ctgaacaggc tggcggcgct aaatatcagt
     9481 tcaattccca ccagtgtgga aaccagcgtt acagacacca tcggcgttgc accaaggaat
     9541 atccaggcaa tgacgatatc cagcacacca ataacgagct gtagccagct gcctttcatt
     9601 gaacgctgac gataccaact catcaggcga ataacccctg caacacagaa caaaccggca
     9661 ataaatgccg caatggcaaa aatgcccagc tccggtgcgc ggatgaagaa atagccgatc
     9721 aataaatagg cgactgcgac gaggaaaccg gataataccg gccagaaatt atgactgcgg
     9781 ttgctgaata acccgacaat aagcgcaatg cccgagcaga ttaataatgc acccactact
     9841 gtgcttaaaa tatcgccaga gacgaacggg aaactgatac acagcaaccc gacgataaac
     9901 agtagcacgg caataaactg gattgctctg cgatgttttt taagcatctc cagatcaaac
     9961 ttcaaaattg ttgccttatc tatatataac atagaaccac cctataaaat taagaagaaa
    10021 atcccctgct atcaatctat gccaaaaacg cgtctaagaa tgtagtcgat ttaataaaaa
    10081 tttcctaatt acagtatctg atgcatctgt aactcattgt attgaaataa aaatatctga
    10141 ttttgatatt ttatatcaac atgacatata cagaaaacca ggttataacc tcagtgtcga
    10201 aattgattcg tgacggctct ttcactttat agttgaggat attacgatga aaaaagtatt
    10261 aggcgttatt cttggtggtc tgcttcttct gccagttgtg agcaatgcag cggatgcgca
    10321 aaaagcagct gataacaaaa aaccggtcaa ctcctggacc tgtgaagatt tcctggctgt
    10381 ggacgaatcc ttccagccaa ctgcagttgg ttttgctgaa gcgctgaaca acaaagataa
    10441 accagaagat gcggttttag atgttcaggg tattgcaacc gtaaccccag ctatcgttca
    10501 ggcttgtact caggataaac aagccaactt taaagataaa gttaaaggcg aatgggacaa
    10561 aattaagaaa gatatgtaat tccgggaatg cgttacatcg tacttccttg catattgaac
    10621 aggccggaat atcttcttta aaagcagcta ttcctcctgt tcatatataa tctctatatt
    10681 gaatgggtta caaaatgaat atttcatctc tccgtaaagc gtttattttt atgggcgctg
    10741 tagcggcttt gtcactggtg aacgcacaat ctgcgctggc agccaatgaa tccgctaaag
    10801 atatgacctg ccaggaattt attgatctga atccaaaagc aatgaccccg gttgcatggt
    10861 ggatgctgca tgaagaaaca gtatataaag gtggcgatac cgttacttta aatgaaaccg
    10921 atctcactca aattcctaaa gtgatcgaat actgtaagaa aaacccgcag aaaaatttgt
    10981 ataccttcaa aaatcaagca tctaatgact tgccgaatta atgaggtaca agtaaaaagg
    11041 agtagcaagt tgagccatct tgctgctcct ttttgcattt ttatatgaca gcagaattta
    11101 ttatacgtct tatactcgcg gcaattgcct gtggcgctat tggcatggaa aggcaaatgc
    11161 gcggcaaagg agcagggtta cgcacacatg tattaattgg catgggaagc gccctgttta
    11221 tgattgtttc gaaatatggt tttgctgacg tgctgtcttt agatcacgtc ggactcgacc
    11281 ccagccgtat tgctgctcag gtggtgacgg gcgtcgggtt tatcggtgca ggtaacattc
    11341 tcgttcgtaa ccaaaatatt gtaggtctga cgacggcagc ggatatctgg gtgaccgccg
    11401 ccataggtat ggttattggc agcggtatgt acgaactggg tatttatggt tcagtgatga
    11461 ccttgttggt gctggaagtc ttccatcaat taaccttccg cctgatgaac aaaaattacc
    11521 atttacagct gacattagtg aacggtaata tggtatcaat gctcgactgg tttaagcagc
    11581 aaaaaataaa aactgattta gtttcgttgc aggaaaatga agatcatgaa gtggtcgcca
    11641 ttgatatcca gcttcatgcc acaacgtcga tagaagacct gttgcgatta ttaaaaggta
    11701 tggcaggggt gaaaggcgtt tcaattagct aatctgtgtg gcagcgtagc cagactcacc
    11761 gtaagcctga aattcacacc agataatcaa tatgctgatg gcgtaccagc tcattgatcc
    11821 gcttaagatt gaggatcatc atgatattgt gtttatggca gtagaacgtt tttagcgata
    11881 ttttcagttt ctccagaata tcttcagttg tcatttgatt cgcaatgcag ctcagaacct
    11941 gatgttgctt gcgcgaaagt gacacattcg cttcaggcac agagcagtaa ctgccattga
    12001 tgagtgcgac aaaatcaatg atagtgatag tccgcggaac aaaggtcacc ggtactttcc
    12061 ccagacagcg tttaatatta aagggggaga aaacaatgac atggacaggt tttaatcgtt
    12121 caatttgagt gagaaaatta gaatgaaata cattattcat taatgtatca ataatgacga
    12181 aggcattctg atgcaacatt acgtcgatct cttcttcgtt ctgtatatga acgacattac
    12241 ctttactcag aatgtttttc atcgcggtga agaacatcgt atccctggta attataagaa
    12301 acataaccga ctcctggtgc ggacattaac tttataatca atttgccaaa ggtaattaac
    12361 aggacagggc ttataataat aatagttagc ttactgtata ttaattttat gtaatacatg
    12421 atgcagatgc tgagaaagca tctgcatctt tcggtggtgt tatttgacca gctcaggtgt
    12481 tacctgactc accgcattgg tgtagtaagg cgcaccccag cccggttccg gccagaatgc
    12541 accatagccg taatcccaca ggttataggt ggtattaacc acttctctca aatgccacac
    12601 ctggatgccc tgcatattca cttccaggaa gttatacggg actttattga taaagccagg
    12661 ttgttcaccc tgaatggtac cgaggatggt aacaaagtga ttacgatagt tcactgggtc
    12721 aaggaagccg ctttgtctgg cgagcagtcg gccctgatag ttggcttcaa tatcaggttt
    12781 cgcatagcta tccaacggta atacagcgat ttctaacaac gtatccgttt tgccattgat
    12841 aacgttgata accttcccac caaagcgcgc ttgttgacca acatataacc ccggctggtt
    12901 atgaacagca acaaaacttt tttgaatatc aggttggtta ttgcctttga tattttgcgg
    12961 aattgaacta catgcggcaa gcaaaaatga aaggctgagg atgagtgcac cttttgtcat
    13021 gttcatgtta ctatccttat caacaaatat taaaaccata atagatattt ctttatagtt
    13081 ttcatctgat tctgaggcga tatgcctgat agtaacgtaa acctgtctga tgtttatatc
    13141 ccttttgttt agttcaattc cattgatttt ggggcgtggc atgagtatcc atcgccccat
    13201 aatataatcc
//
LOCUS       DAETQN010000121        12998 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.121, whole genome shotgun sequence.
ACCESSION   DAETQN010000121 DAETQN010000000
VERSION     DAETQN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.121"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            98..1393
                     /locus_tag="K0W68_003438"
     CDS             98..1393
                     /locus_tag="K0W68_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBI3074904.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="K0W68_003439"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="K0W68_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBI3074905.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="K0W68_003440"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="K0W68_003440"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBI3074906.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="K0W68_003441"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="K0W68_003441"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBI3074907.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="K0W68_003442"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="K0W68_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBI3074908.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="K0W68_003443"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="K0W68_003443"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBI3074909.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="K0W68_003444"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="K0W68_003444"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBI3074910.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="K0W68_003445"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="K0W68_003445"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBI3074911.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3040 a         3228 c         3577 g         3153 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggcgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc atcaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gattgcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctagtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgcgggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAETQN010000122        12872 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.122, whole genome shotgun sequence.
ACCESSION   DAETQN010000122 DAETQN010000000
VERSION     DAETQN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12872
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.122"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..696)
                     /locus_tag="K0W68_003446"
     CDS             complement(<1..696)
                     /locus_tag="K0W68_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI3074912.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="K0W68_003447"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="K0W68_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBI3074913.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="K0W68_003448"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="K0W68_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBI3074914.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="K0W68_003449"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="K0W68_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBI3074915.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="K0W68_003450"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="K0W68_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBI3074916.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="K0W68_003451"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="K0W68_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBI3074917.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="K0W68_003452"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="K0W68_003452"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBI3074918.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="K0W68_003453"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="K0W68_003453"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBI3074919.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="K0W68_003454"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="K0W68_003454"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBI3074920.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="K0W68_003455"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="K0W68_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBI3074921.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="K0W68_003456"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="K0W68_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBI3074922.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="K0W68_003457"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="K0W68_003457"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBI3074923.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /locus_tag="K0W68_003458"
     CDS             10119..10499
                     /locus_tag="K0W68_003458"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBI3074924.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="K0W68_003459"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="K0W68_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBI3074925.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="K0W68_003460"
     CDS             complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="K0W68_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBI3074926.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11470..12660
                     /locus_tag="K0W68_003461"
                     /pseudo
     CDS             11470..12660
                     /locus_tag="K0W68_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         3278 a         3147 c         3354 g         3093 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggctc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccatg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagctt ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 gctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcgagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataact
    10861 ataaataagt gtcttggcag atactatata aattaactta gtgaatgatt atgctaatgt
    10921 catcaattaa ataaatataa tggcgttaag gcttcccagt aatataatta atactctact
    10981 tccagagtag aatattaaat tttatccgcg tggtgcatca gcacaaattt atcccacaac
    11041 tgttcttctg tctcgacatg cgccggatct ttcacaatag tattggggat cgggcacacc
    11101 ttctggcagg ttggtgtctc gtagtgccct acgcattcgg tacacttatc gctgttaatc
    11161 tcgtagatat gatctcccat tgaaatcgcc tcattcgggc attcgggttc acacatatca
    11221 caattaatac agcgtttagt aattagtaaa gacatttcaa tggattaccg ttaaatcatt
    11281 ttaaaatcag taagttgtat cgagtttgta tgctttactg tcattaactt actgtatgtt
    11341 gatccagtgt atttaacctt gataaactca gtccagcaac acaaaaccgc aacacattgc
    11401 attttgtccc gtagaaaaga cttgtatgtg tgagcttgtt ttctgcgcct acgcagataa
    11461 ggattgagaa tgccgcgcac tgtaacacat aatccggata gccccaataa tgacgatgtt
    11521 ttagccgcat ctgaaaaatg ggacgcctgt aaacccccct ataccagcgc acacatgaaa
    11581 atctgtgttg ctgccgccaa aatcatcctc gctgcttccg gcgtggctcg ccgttccaaa
    11641 tacgaaaaag agaactatct ccgtatcgat ttcagcaaag ccggtaaggt tacattttac
    11701 gccgagtttc caaaaaagat gggcctcaaa ggtaaaaagc tcggcgagtg gccggagctc
    11761 gctatccagc tggcgcgcga aaaagcacta ggtatggctg acggtggact gcgggcagag
    11821 tccgtacatg cagcgctgga aatgtaccgg gatgacctca aagccaaagt agcccggcag
    11881 aagctgagcc cggacagttt cacaacctac ggggtgcgta tcgaccggat taaagcaacg
    11941 ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc ggctggtgga agtgctggac
    12001 gagtggatcg ccactcgctc gaacaataac gccctggagt tgtttgccga gctccgtcgg
    12061 ttctggaagt tctgcgcacc tactctttgc aacggccgca atgttgccgc cagtctgcca
    12121 gatgattatg tttcctcccg cgtacagaaa cctaccccca cacggctttt taccgatatt
    12181 gaatcaatcg cccgactctg gctcaatgtt gctgcctgca cctctgtaca ccagaagaat
    12241 gctgttcgct tcatgatcat cactggtgtt cgtccgatta atgtccataa cctgcgctgg
    12301 gactacgttt acgaggaggc tggtgaaatt gtttatccgg aaggggttat cggcatgcga
    12361 ggggctatga aaacacaaaa ggctttccgc ctgccgataa cgcctgagat ccggcggatt
    12421 atcgacgagc agaaagcctg gcgtgattca gttcctgagt gcaacaggga ttatgtattt
    12481 ttgcagccac gtgatccaat gcagccattt tcaaaacgat cactggataa gctggtgaaa
    12541 acatacagcc cggacggggc tgtaaaagga ataaaacatg atgggactgt taaagggaaa
    12601 gacggtgcat ttaatacgat gtgccgtaaa ttccttaaga gcaatgttat tgccttgatc
    12661 gataggaatt aaaaccccaa aaagattaaa aaaacaccac aaaacggatg tttcttcaac
    12721 accacttttg ctcatatgaa cggaaccgac gattaaactg gatggctctg attgattcag
    12781 ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg gacgcaactt tccctctgcg
    12841 gctctcagcc gcaccaccgc atccgggcca gc
//
LOCUS       DAETQN010000123        12359 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.123, whole genome shotgun sequence.
ACCESSION   DAETQN010000123 DAETQN010000000
VERSION     DAETQN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.123"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(121..330)
                     /locus_tag="K0W68_003462"
                     /pseudo
     CDS             complement(121..330)
                     /locus_tag="K0W68_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="K0W68_003463"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="K0W68_003463"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBI3074927.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="K0W68_003464"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="K0W68_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HBI3074928.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="K0W68_003465"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="K0W68_003465"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HBI3074929.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="K0W68_003466"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="K0W68_003466"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBI3074930.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLMDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="K0W68_003467"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="K0W68_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HBI3074931.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="K0W68_003468"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="K0W68_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HBI3074932.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="K0W68_003469"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="K0W68_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBI3074933.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="K0W68_003470"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="K0W68_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBI3074934.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="K0W68_003471"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="K0W68_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBI3074935.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="K0W68_003472"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="K0W68_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBI3074936.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="K0W68_003473"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="K0W68_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBI3074937.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="K0W68_003474"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="K0W68_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBI3074938.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         2831 a         3451 c         3021 g         3056 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagta acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg atgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccatcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag tacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat gacacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaact
//
LOCUS       DAETQN010000124        12037 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.124, whole genome shotgun sequence.
ACCESSION   DAETQN010000124 DAETQN010000000
VERSION     DAETQN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.124"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            9..323
                     /locus_tag="K0W68_003475"
     CDS             9..323
                     /locus_tag="K0W68_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBI3074939.1"
                     /translation="MLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIAIF
                     AVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPASN
                     VD"
     gene            457..1534
                     /gene="ygjJ"
                     /locus_tag="K0W68_003476"
                     /pseudo
     CDS             457..1534
                     /gene="ygjJ"
                     /locus_tag="K0W68_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1551..3902
                     /gene="ygjK"
                     /locus_tag="K0W68_003477"
     CDS             1551..3902
                     /gene="ygjK"
                     /locus_tag="K0W68_003477"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBI3074940.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            4128..6146
                     /gene="fadH"
                     /locus_tag="K0W68_003478"
     CDS             4128..6146
                     /gene="fadH"
                     /locus_tag="K0W68_003478"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBI3074941.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6191..6607)
                     /gene="higA"
                     /locus_tag="K0W68_003479"
     CDS             complement(6191..6607)
                     /gene="higA"
                     /locus_tag="K0W68_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBI3074942.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6604..6918)
                     /gene="higB"
                     /locus_tag="K0W68_003480"
     CDS             complement(6604..6918)
                     /gene="higB"
                     /locus_tag="K0W68_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HBI3074943.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="K0W68_003481"
     CDS             complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="K0W68_003481"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBI3074944.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8424..8927
                     /locus_tag="K0W68_003482"
     CDS             8424..8927
                     /locus_tag="K0W68_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBI3074945.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9004..9687
                     /locus_tag="K0W68_003483"
     CDS             9004..9687
                     /locus_tag="K0W68_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBI3074946.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9766..10752
                     /locus_tag="K0W68_003484"
     CDS             9766..10752
                     /locus_tag="K0W68_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBI3074947.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            11035..12000
                     /gene="alx"
                     /locus_tag="K0W68_003485"
     CDS             11035..12000
                     /gene="alx"
                     /locus_tag="K0W68_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBI3074948.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2862 a         3094 c         3225 g         2856 t
ORIGIN      
        1 cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta cgcgccaaat
       61 tagatcacct gccacgcgat ttccgtatgg gctcccgccg caccggtatt atcgttgttt
      121 caatgctgat tgcgatattt gccgtagggt ttgtcgcttc gacattcccg actggcgcga
      181 atattctgac catcattttt tataacgtcg gcggtattgt tatcttcctt ggctttgcgt
      241 ggtggaaata cagtaaatat ataaagggat taacggctga agagcgccat attgaagcga
      301 cgccagccag caatgttgat taagcagaat aataaaaaat gagcaggcat tttccctctt
      361 cctggatgag gagagggaaa atagtttctg cctttatatt tttataatta caacgataaa
      421 aggctgtact ttttctttag ctcatggatt aacacaatga aattaatcac tgcaccatgc
      481 agagcattac ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt
      541 ccggcagaac atgacgacac aaaaacaccc gcaattacct cgacatcttc tccttcattt
      601 cgtttttacg gcgaattagg gattaggggg tggtggatat atggatttag agggtgagaa
      661 taaacataaa tacagcgacg gtacctatat tgaaggtggc ctggagatga agtacggctc
      721 ctggttcggc ctgatttacg gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc
      781 ctgggtgcca gaccatagct ggggtggttt cgagggcgga attaaccgtt tctatggcgg
      841 ttatcgtacc aatgatggca ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga
      901 tgacctgcaa tggtggggcg atttcacccc cgatctgggc tacgtcattc ccaatacccg
      961 cgacattatg actgcgctga aggtacagaa cttaagcggc aactttcgtt atagcgtcac
     1021 cgcgactcct gccggacatc atgatgaaag caaagcctgg ctacattttg gcaaatacga
     1081 tcgctatgac gacaaataca cctatccggc aatgatgaac ggttacatcc agtatgacct
     1141 tgccgaaggc atcacctgga tgaacggtct ggaaatcacc gacggcacag gacagctcta
     1201 tctcacgggc ctgctaactc ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc
     1261 cgacgggctg gacgtaccgg gaagtgaaag tgggatgatg gtgagcgcca tgtatgaagc
     1321 gttaaagggc gtttatctct ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc
     1381 tgacgatgaa accacctctt tcatgcagtt tggtatctgg tacgaatacg gcggcggacg
     1441 tttcgccacg gcttttgata gccgcttcta catgaaaaat gcctctcacg atcccagcga
     1501 ccaaatcttc ctgatgcaat atttctactg gtaataagga ctgtaatttc atgaaaataa
     1561 aaactatttt aacgccagta acctgcgctc tgctgataag tttttccgcc catgccgcta
     1621 acgccgacaa ttataaaaac gtgattaacc gtactggcgc gccgcagtac atgaaggatt
     1681 acgattacga cgatcaccag cgttttaatc cgtttttcga tctcggagcc tggcatggtc
     1741 atctgttgcc agacggccct aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg
     1801 aagagtacat caactttatg gccagcaatt tcgaccgcct gaccgtctgg caggacggca
     1861 agaaagtcga ctttacgctg gaggcataca gtattcccgg tgcgctggtg caaaaactga
     1921 cagcaaaaga tgtgcaagtc gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac
     1981 tggaaaccaa aatcaccagc aataaaccgc tggatctggt gtgggacggc gaactgctgg
     2041 aaaaactgga agcgaaagaa gggaaaccgc tttccgataa aaccattgct ggcgaatacc
     2101 ctgactatca gcgcaaaatc agcgccaccc gtgatggcct gaaagtcacc tttggcaaag
     2161 tgcgcgccac ctgggatctg ctgacctccg gcgaatcgga atatcaggtg cataaatccc
     2221 tgccggtgca gactgaaatc aacggtaatc gctttaccag taaggcgcat atcaacggtt
     2281 cgaccacgct ctacaccacc tattcccatc tgctgaccgc tcaggaagtt agcaaagagc
     2341 aaatgcagat ccgcgatatt ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac
     2401 gctgggaaga atacctgaag aaagggttaa ccaatccgga tgcgacgccg gaacagacgc
     2461 gcgtcgcggt gaaagcgatc gaaacgctca acggtaactg gctctcaccg ggcggtgcgg
     2521 tgaaatttaa caccgttaca ccgtcggtga ccgggcgctg gttctccggc aatcagacct
     2581 ggccgtggga tacctggaag caggcgtttg cgatggcgca tttcaatccg gacatcgcca
     2641 aagagaatat ccgcgcggtc ttctcctggc agatccagcc tggcgatagc gtgcgtccgc
     2701 aggatgtggg ctttgtcccc gacctgatag cgtggaacct tagccccgag cgtggtggcg
     2761 atggcggcaa ctggaacgaa cgtaatacca aacccagcct tgccgcctgg tcggtgatgg
     2821 aagtgtacaa cgtcacccag gataaagcct ggttggcaga gatgtacccg aaactggtgg
     2881 cctatcacga ctggtggtta cgtaaccgcg atcacaacgg caacggcgtg ccggaatatg
     2941 gcgcgacccg cgacaaagcc cacaacactg agagcggcga gatgctgttt acggtgaaaa
     3001 aaggcgataa agaagagacg cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag
     3061 gcctgtacga cagtctggaa attccggcac aggttgctgc atcgtgggaa tcggggcgtg
     3121 atgatgccgc cgtctttggg tttatcgaca aagaacagct ggataaatat gtcgctaatg
     3181 gcggcaaacg tagcgactgg acggtgaaat tcgccgaaaa ccgcagtcag gacggaacgt
     3241 tgctgggcta ctcgctattg caggagtcgg tggatcaggc cagctatatg tacagcgata
     3301 accattatct ggcggagatg gcaaccatcc tcggtaagcc agaagaggcc aaacgctatc
     3361 gccagttggc acagcagctc gcggactaca tcaacacctg tatgttcgac ccgactaccc
     3421 agttctacta tgacgtacgt attgaagata aaccactggc gaacggctgc gcgggcaaac
     3481 cgattgttga gcgcggtaaa gggccggaag gctggttgcc gctgtttaac ggtgcggcaa
     3541 cgcaggccaa cgccgacgcg gtggtgaagg tgatgctcga tcctaaagag ttcaacacct
     3601 ttgtcccgct gggaacggcg gcgttaacca atccggcttt tggcgctgat atctactggc
     3661 gtgggcgcgt atgggtggat cagttctggt ttggtctgaa agggatggag cgttacggtt
     3721 atcgtgatga tgccctgaag ctggcggata cgttcttccg gcacgccaaa ggattaaccg
     3781 ccgatggccc aattcaggag aattacaacc cgctgactgg cgcacagcaa ggcgcaccaa
     3841 atttctcctg gagtgccgct catttgtata tgttgtataa cgattttttc cgtaagcagt
     3901 aatttgcaac ggctggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc
     3961 ccaggccgga taaaacgcgg caagcgttgc atccggcgtt accaccgggc gtattctttt
     4021 tgaatcccat cacaaacccc gcactcccct tttccctttt ctccatcgac ggctaaatta
     4081 gaactcatcc gaccacataa caattatttt acatactgga caattttatg agctatccgt
     4141 cgctgttcgc cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct
     4201 caatgcacac cgggctggag gaatacccgg acggtgccga gcggctggca gcgttttatg
     4261 ccgaacgcgc ccgtcacggc gtggcgctga ttgtcagcgg cggcattgca ccagatttaa
     4321 caggcgttgg catggaaggt ggcgcaatgc tcaacgacgc cagccagatc ccacaccatc
     4381 gcaccattac cgaagcggta catcaggaag gcggcaaaat agccctgcaa attttgcata
     4441 ccgggcgcta cagctaccaa ccgcatctgg tcgctccctc cgcattgcag gcccccatca
     4501 accgtttcgt tcctcacgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg
     4561 cccactgcgc gcaactggca cgggaggcag gatacgacgg tgtagaggta atggggtccg
     4621 aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa tcagcgtagt gaccagtggg
     4681 gcggcgatta ccgcaaccgg atgcgatttg ccgtagaagt agtgcgtgcg gtgcgcgaac
     4741 gcgtcggcaa cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaagacg
     4801 gcgggacttt tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca
     4861 ttatcaacac cggcattggc tggcatgaag cacgtattcc gaccattgcc acgcccgtgc
     4921 cgcgcggcgc atttagctgg gtcacgcgca aattgaaagg ccacgtctcg ctgccgctgg
     4981 taaccaccaa ccggattaac gatccgcagg ttgccgacga tattctctcg cgcggcgatg
     5041 ccgatatggt atcgatggca cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc
     5101 aatcggggcg agccgatgag atcaacacct gcattggctg caatcaggcc tgcctcgatc
     5161 agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgc cacgaaacca
     5221 aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg
     5281 ctgggctggc gtttgccatt aacgcggcgg cgcgtgggca tcaggtaaca ttgtttgatg
     5341 cccacagcga gatcggcggg cagtttaata tcgccaaaca gatccccggc aaagaggagt
     5401 tttacgaaac gctgcgctat taccaccgga tgatcgaagt gacgggcgtg acgctgaaac
     5461 tcaatcacac cgtgacggcg gatcagttac aggcgttcga tgaaacgatc ctcgccagtg
     5521 ggatcgtgcc gcgcgttccg cctatcgacg ggatcgatca tccgaaggta ttgagttatc
     5581 tcgatgtact gcgcgacaaa gcgccggtag gcaacaaggt tgccatcatc ggttgtggcg
     5641 ggattggttt tgatacggcg atgtatttaa gtcagccggg cgaatccacc agccagaata
     5701 tcgccaggtt ctgtaatgag tgggggatcg acagcagcct acaacaggct ggtggcttaa
     5761 gcccgcaggg aatgcagatc ccgcgtagcc cacggcaaat tgtgatgctc cagcgcaaag
     5821 ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc
     5881 tctcgcgggg tgtgaaaatg atcccaggcg taagttatca gaagattgac gatgacgggc
     5941 tgcatgtggt gatcaacggc gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg
     6001 cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc
     6061 atttaattgg cggctgcgat gtggctatgg agctggacgc acgacgggca attgcccagg
     6121 gaacacggct ggcgctggag atttaaatcg ttattttaag cgccggatgc aacgcatccg
     6181 gcacgtcgcg ttaatcaata aacaaggcgg gagaaatgcc gaatcgcgtt gccaattttt
     6241 tagcgtgttc cagcgttaat ttcctcttcc cgctcaaaac gcgtgacacc atagatttac
     6301 tgccaatttc cggcagatcg gcaagggtta aaccatattg atccataagg gtacgaatca
     6361 cggctatacc gccaggcatg gcttgagcca tggcattaaa ttccgcaaat tcgggcgctg
     6421 attcttccca cgcggttatt ttggcacaca ccagatccag caaggggttt tcaggatcgt
     6481 tgagcagcag atgatctacc agttccagcg cctgggtgta ttgttcctcg ttctgaatac
     6541 ccgccagaaa aggtgccacc gcagttagct tttctcctgc ttgcaagatg tcggcaatcg
     6601 caatcatttt ttccccttag tacgatgaac agcggtaaag aaatcgtatt ctttatgcgt
     6661 cataacttca cgtatgtagc acttttgcga ttcaaaaaag accattgcta caacacgtaa
     6721 ttcattgccc ccaacattga aaacataatg cttatccaga tatttgaagt tatccagaga
     6781 tgggaatact gcttttaatg actcaggttt tttgaaatat cccttagcaa tcgtgttccc
     6841 cagagccacc aactccgttt tatgttgcgg gtatttttcc gcagcatctt tcaatgcttt
     6901 ttgagttatc aggtgcattc ttcatcacgt ccgttgacaa attggcaata tgataacatc
     6961 cgttgacaga ttggcaacgg atgaatttat ctgtgtgacg gaagaataaa cgtagaaaaa
     7021 cgctaaacaa gagaatgtag aggatggtga gagggatttt ttgttttatg gaagcgcgat
     7081 tccagaactg ggcagacatt gccggatgcg acgcttgccg cgtcttatcc ggcctgggag
     7141 taacgtgctg aacgtaggcc ggataaggcg ttaacgccgc atccggcagg taactcccaa
     7201 tcttaccgac gacgccccag cttcactgct ttcagcacca caaatttatt attcgtggcg
     7261 atggtggtgc agttgccgaa aatcttcttc agtttatgga agtaatccag gtgacggttg
     7321 gcaacaatat acagctcgcc gttgattttc aggcagcggc gggcatggtg gaacatctcc
     7381 caggcgacgt tatcggtcag cgcatgttgt tggtgaaacg gcgggttgca gagcacagca
     7441 ttaaagcgga aaggctccac gccggagagc gcgttgttga tcataaactc gctgcgatct
     7501 aacgcctctg gcatgttggt ttcaacgttc aaacggctgg aagcaaccgc catcggcgat
     7561 tcatcgacaa acaccacttt cgcctgaggg tttttatcaa gcagcgtcag accaataacg
     7621 ccattaccgc aaccgagatc gacaatctca ccttcgagat tctctggcag atgttgcata
     7681 aagaagcgtg cgccaatatc cagcccggtg cgggagaaga cattcgcatg gttgtggata
     7741 gtccagtcag taccttccag cttccagcta acggtctgcg gcgcatcggc cagcggcggt
     7801 tcattgaaag tgcaattaat caggcgcgct tttttccatg ccagcgtagt ggtggtcggg
     7861 ccgagcactt tctcgaacag ctccagcgtg gaagtgtgaa tgtcacgggc tttggcaccg
     7921 gcaataatac gtgtatccga cgtgaccact ttgcgcagcg cacgcagttg ctgttccagc
     7981 aatgccagtg ttttcggcac tttgatcagc accacacccg gctgttgcgg gtagtcggcg
     8041 gtgctgtcga gaaacttcac gctcgactca tcaatcccgt tgaggcgtaa attctcgcgc
     8101 gtcgccagtt cactgatgta tgagtcgcca atgctgtacg gcttatgttc cgccagcgcg
     8161 cagcttaacg caccaaaggc atcattcagg atcaacaccg ggccgcggat ttctgtgtcg
     8221 tccaactgtt gcagcaaata ttcatccgcc gcttcccacg cctgtagcgg gttaacgtca
     8281 tccgtcgccg gaaaacgttg tagtgtcagt gaacggaaac cgttgtctaa gtggctcatc
     8341 ggccctcctg aatgataaaa tttcgacgtt gcccctgaaa agggtgcgtg agtataccct
     8401 ttttctcttt ttcgtgggcg gttatgagca atcttactta tcttcagggt tatcccgagc
     8461 agctactttc ccaggtgcga acgctgatta acgaacagcg tctgggcgat gtgctggcaa
     8521 aacgctatcc gggaacacac gactacgcca ccgataaagc cctctggcaa tatacccagg
     8581 atctaaaaaa tcagtttctg cgcaatgccc cgccgatcac taaagtgatg tatgacaata
     8641 agatccacgt actgaaaaac gcgctcggat tacataccgc tgtttctcgt gtgcagggcg
     8701 gcaagctgaa agcaaaagca gagatccgcg tcgctactgt gtttcgcaac gcgccggaac
     8761 cgtttttacg catgatcgtg gtgcacgaac tggcgcacct gaaggagaaa gagcataaca
     8821 aagcgtttta ccagttgtgc tgccatatgg agccgcagta ccaccagctt gagttcgaca
     8881 cccggctgtg gctaacgcag ttgtcgcttg ggcaggacaa aatctgaaaa tgatgtcagg
     8941 cgttatcttt tctttcttta gcgcctaaaa tcgacctccc ccctttcgtt aaggataacg
     9001 accatgcagc cccgccttct tctccgtatt tgtttttctc gccggactct aaagattacc
     9061 tgcttactgt tacttgtggc aggagcgacc atttttatcg ccgatcgcgt aatggtcaac
     9121 gccagcaagc agctgacctg gagcgatgtc aacgcagttc cggcgcgtaa cgtgggttta
     9181 ttgctggggg cgaggccggg taatcgctac tttacccgac gtattgatac cgcagcagcg
     9241 ttgtaccacg tcggaaaagt gaaatggctg ttagtcagtg gcgataacgg acgtaaaaat
     9301 tacgatgaag cctcaggtat gcagcaggct ttgatcgcta aaggcgtacc ggcgaaagtg
     9361 atcttctgcg actatgccgg attctcaacg cttgattcag tggtgcgtgc caaaaaggtg
     9421 tttggggaaa accatatcac cattatctcg caagaatttc ataaccaacg cgccatctgg
     9481 ctggcaaaac agtacggcat cgacgctatc gggtttaacg cgccagacct taatatgaag
     9541 cacggttttt atactcaact gcgggagaaa ctcgctcgtg tcagcgcggt gattgacgcc
     9601 aaaatcctcc atcgccagcc gaaatatctc gggccatcgg tcatgattgg gccgtttagt
     9661 gagcatggct gtccggcgaa agaataatgc gtatctgcgc acgtcgaaga tgaaaaaggc
     9721 gtgctacatt gacgacagaa tccctttatg gagtatccac gcgttatgat acgtttcgct
     9781 gtgattggta cgaactggat cactcgccag ttcgtcgagg ccgcccatga gagcggtaaa
     9841 tacaagttaa ccgccgtata ttcccgcagc cttgaacagg cccagcactt cgccaatgat
     9901 ttttctgtcg agcatctgtt tacctcgctg gaagcgatgg cggaaagcga tgccattgac
     9961 gcggtgtata ttgccagccc gaatgccctg cacttttccc agacacaact tttccttagc
    10021 cataaaattc atgtgatttg cgagaaacca ctggcgtcga atctggcgga agtggatgcc
    10081 gccattgcct gtgcgcggga aaatcaggtg gtgctgtttg aggcatttaa aaccgccagc
    10141 ctgccgaact ttcatttgct gcgccaggcg ctgccgaaag tcggcaaatt gcgtaaagtc
    10201 tttttcaact attgccagta ttcctcgcgg tatcaacgtt acctggatgg tgagaatccc
    10261 aacaccttta atccggcatt ctctaacggt tcaattatgg atatcggctt ttactgcctg
    10321 gcgtcggcgg tggcattatt cggtgaaccg aaaagcgtgc aggcaaccgc cagtttgttg
    10381 gcaagcggcg tagatgccca cggcgtggtg gtgatggatt acggtgattt cagcgttacg
    10441 ttgcagcact caaaggtcag tgattctgtc ctggaaagcg agattcaggg cgaagcggga
    10501 tcgctggtga ttgaaaaact gtctgaatgc cagaaagtgt gcttcgtgcc gcgtggcagc
    10561 cagatgcagg atctcaccca gccgcagcat attaatacca tgctctacga agcagagctg
    10621 tttgctaccc tggtggatga gcatctggtg gatcatccag ggctggcggt cagtcgcatc
    10681 accgccaaac tgctgactga gatccgccgc cagactgggg tgatttttcc ggcagatagc
    10741 gtaaaactat aattgccaaa gtaaaacagt gtaaaaggta tgtaacagac cattgactgg
    10801 ctgaatggtc tgtcatactt tgttacctgc aaaggggagt aacttcattg ccggtcgatc
    10861 gtcattacga tgtgtgaaaa accacatccg gtcaccgggc aacccgaaag gaatacgcag
    10921 acgtattcct tttttgttgt aagtgagacc ttgccggaag gcgaggtcta tgcataaaaa
    10981 gcagcggctg acgtcttccg acgttggccg tttttttatg tgtaaggaac ttctatgaat
    11041 actgtcggca cgccgttgct atggggcgga ttcgctgttg ttgtcgccat tatgctggct
    11101 atcgacctgt tgttgcaggg gcgtcgtggg gcacatgcca tgaccatgaa acaggctgcg
    11161 gcctggtcgc tggtctgggt gacgctgtcg ttactgttta acgccgcttt ctggtggtat
    11221 ctggtgcaaa ctgaaggtcg cgccgttgcc gatccacagg cactggcctt tctcacgggt
    11281 tatctgattg agaaatcgct ggcggtcgat aacgtctttg tctggctgat gttgttcagc
    11341 tatttctctg ttccggcggc attacaacgc cgcgtgctgg tgtatggcgt gctcggggcg
    11401 attgttctgc gtaccatcat gatcttcact ggcagctggc taatttcgca gttcgactgg
    11461 atcctgtata tcttcggtgc cttcctgctg tttaccggcg tgaagatggc gctcgcccat
    11521 gaagatgaat caggcattgg cgacaagccg ctggtgcgct ggctacgcgg tcatttgcgc
    11581 atgaccgaca ccatcgacaa cgagcatttc tttgtgcgta agaatggatt gctgtacgcc
    11641 acaccgctga tgctggtact gattctggtg gaattgagcg acgtgatttt cgccgtggat
    11701 agcattccgg ctatcttcgc cgtgaccact gacccgttca ttgtgctgac ctcaaacctg
    11761 tttgcgatcc tcggcctgcg tgcgatgtat ttcctgctgg cgggcgtagc agagcgtttc
    11821 tcgatgctca aatatggcct ggcggtgatt ctggtgttta tcggtatcaa gatgctgatt
    11881 gtcgacttct accatattcc aatcgccgtc tcgctgggcg tggtgtttgg cattctggtg
    11941 atgacgttta ttatcaacgc ctgggtgaat tatcggcatg ataagcagcg gggagaatga
    12001 ttcgctgtat attaagccat tcctagagat aactcat
//
LOCUS       DAETQN010000125        11960 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.125, whole genome shotgun sequence.
ACCESSION   DAETQN010000125 DAETQN010000000
VERSION     DAETQN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.125"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1760
                     /locus_tag="K0W68_003486"
     CDS             <1..1760
                     /locus_tag="K0W68_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBI3074949.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
     gene            2096..3073
                     /gene="csiD"
                     /locus_tag="K0W68_003487"
     CDS             2096..3073
                     /gene="csiD"
                     /locus_tag="K0W68_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBI3074950.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3093..4361
                     /gene="lhgO"
                     /locus_tag="K0W68_003488"
     CDS             3093..4361
                     /gene="lhgO"
                     /locus_tag="K0W68_003488"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBI3074951.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4384..5832
                     /gene="gabD"
                     /locus_tag="K0W68_003489"
     CDS             4384..5832
                     /gene="gabD"
                     /locus_tag="K0W68_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBI3074952.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5846..7126
                     /gene="gabT"
                     /locus_tag="K0W68_003490"
     CDS             5846..7126
                     /gene="gabT"
                     /locus_tag="K0W68_003490"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HBI3074953.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7437..8837
                     /gene="gabP"
                     /locus_tag="K0W68_003491"
     CDS             7437..8837
                     /gene="gabP"
                     /locus_tag="K0W68_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBI3074954.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVISASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8858..9520
                     /gene="csiR"
                     /locus_tag="K0W68_003492"
     CDS             8858..9520
                     /gene="csiR"
                     /locus_tag="K0W68_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBI3074955.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="K0W68_003493"
     CDS             complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="K0W68_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBI3074956.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10054..10212)
                     /locus_tag="K0W68_003494"
     CDS             complement(10054..10212)
                     /locus_tag="K0W68_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBI3074957.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10395..10694
                     /locus_tag="K0W68_003495"
     CDS             10395..10694
                     /locus_tag="K0W68_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3074958.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10704..11228
                     /gene="ygaP"
                     /locus_tag="K0W68_003496"
     CDS             10704..11228
                     /gene="ygaP"
                     /locus_tag="K0W68_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBI3074959.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11275..11679)
                     /gene="stpA"
                     /locus_tag="K0W68_003497"
     CDS             complement(11275..11679)
                     /gene="stpA"
                     /locus_tag="K0W68_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBI3074960.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2986 a         2947 c         3077 g         2950 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctagcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa
     1681 ctctaaatat caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc
     1741 cgaactatct ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat
     1801 atgtttagtt tactaacggt cattttgcag tgaagccatt tactgttttt tatcgaccag
     1861 ataatctgtt ctctaatgtt aactccccct aacctgttgc tttagttatt catttcctgt
     1921 ctcactttgc cttaatactc tacgttaaat gttactaatt tgttgctttt gatcacaata
     1981 agaaaacaat atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
     2041 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
     2101 tgcactgacc gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac
     2161 tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac
     2221 caaacagttt ctcgagcagg ttgccgaatg gcccgtacag gcgctggagt acaaatcgtt
     2281 tctgcgtttt cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt
     2341 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtat
     2401 cgatgatgtg gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat
     2461 tggtcgctca aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa
     2521 tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga
     2581 cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa
     2641 catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta
     2701 tttccgccat ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt
     2761 cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg
     2821 ctatatcgac cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct
     2881 ttccgatgcc attgaaacca gcaaaggcat tctttctgta cccgttcccg ttggcaaatt
     2941 cctgttgata aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga
     3001 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactacca
     3061 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     3121 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ttatccggat gcccgcattg
     3181 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     3241 tccatgccgg ggtctattac acgcccggca gcctgaaggc gcagttttgc ctggcgggga
     3301 accgcgccac caaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga
     3361 tgctggtcgc cacgtctcag ctcgaaatgg aacggatgcg cgccttatgg gaacgcacag
     3421 cggcgaacgg tatcgagcgc gagtggataa acgccgtgga actgcgcgag cgcgaaccga
     3481 atatcaccgg gcttggtggc atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     3541 tcacagcggc aatggcaaaa atcttccagg ccagaggcgg cgagattatt tataacgccg
     3601 aagtcagcgc cctcagtaag cataaaaacg gcgtggtgat acgcacccgt cagggcggtg
     3661 aatatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     3721 tgctcggcct cgaaccgggc tttatcatct gcccgttccg tggtgagtat ttccgccttg
     3781 cgccggagca taaccagatt gttaaccacc tgatttaccc cattcccgac cccgcgatgc
     3841 catttttggg cgtgcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     3901 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agtgacacgc
     3961 tggagatttt gagctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     4021 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctacct gcggctggtg caaaagtatt
     4081 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcag
     4141 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccacccca cgcacgatcc
     4201 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     4261 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     4321 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta
     4381 acaatgaaac ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     4441 ctggacgcca acaatggcgg gatcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     4501 ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc
     4561 gccctgcccg cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg
     4621 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggc
     4681 aaaccactgg ccgaagcgaa aggcgaaatc agctacgctg cctcttttat tgagtggttt
     4741 gccgaagaag gcaaacgtat ttatggcgat accattcccg gtcatcaggc cgataagcgc
     4801 ctgattgtta tcaagcagcc gattggcgtt accgcagcta tcacgccgtg gaacttcccg
     4861 gcggcgatga ttacccgtaa agccggtccg gcgctggcgg caggctgcac catggtgctg
     4921 aagcccgcca gtcagacacc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg
     4981 ggcattccgg ctggcgtgtt taacgtagtc accggttcgg caggcgcggt cggtaatgaa
     5041 ctgaccagca acccgctggt acgcaaactg tcgtttaccg gttcgaccga aattggccgc
     5101 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     5161 gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcgctggcc
     5221 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     5281 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc
     5341 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     5401 gtggaagagc atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa
     5461 gcggatgaac gcggcggcaa cttcttccag ccgaccattc tggtggacgt gccggccaac
     5521 gccaaagtgt cgaaagaaga gacgttcggc cccctcgccc cgctgttccg ctttaaagat
     5581 gaagccgatg tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac
     5641 gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc
     5701 atcaataccg gcattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt
     5761 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     5821 atcggtcttt aactggagaa tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag
     5881 tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa
     5941 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt
     6001 gctcaatacc gggcacctgc atccgaaggt ggtggccgca gtggaagcgc agttgaaaaa
     6061 actgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat
     6121 tatgaatcag aaggtgccgg gcgatttcgc caagaaaacg ctgctggtta cgaccggttc
     6181 cgaagcggtg gaaaacgcgg taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat
     6241 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     6301 gaatccgtac tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc
     6361 ttgcccgctg cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa
     6421 aaatgatgcc gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     6481 cggtttctac gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca
     6541 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     6601 tgcgatggag cagatgggcg tggcgccgga tcttaccacc tttgcgaaat cgatcgcagg
     6661 cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg
     6721 cggtctgggc ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     6781 gaaggtgttc gagcatgaaa atctgctgca aaaagccaac gatctggggc agaagctaaa
     6841 agacggattg ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     6901 agcgatgatt gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact
     6961 caccgccgag atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc
     7021 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     7081 gggtctggag atcatcagcc agtgttttgc tgaggcgaag cagtagctat gtccgtttgt
     7141 aagagttaac cgttgctaag ggaatgataa cgatgcagta aaaaataata acgatgaggc
     7201 gaacgacaaa gttaaaaacg actgcggaca gtcccctcgc ccctctggag agagggttag
     7261 ggtgaggggt aaaacacgcg gctaaaccct cccctggccc tctccctaag ggagagggag
     7321 aaaaccctcc cgctttcgtt tttaagattt tgttgaccgt ctcaattaaa cgcgtgaccc
     7381 ggcgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg
     7441 ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa atcacgccac gtcaccatgt
     7501 tgtctattgc cggtgttatc agcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg
     7561 aagcgggccc ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta
     7621 tgcggatgtt ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg
     7681 ccgataaagc cattggtcgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt
     7741 gggtactggt tatcccgctg gaagccaata tcgccgctat gatcctgcac tcgtgggttc
     7801 caggcattcc catctggtta ttttccctcg tcattaccct tgccttaact ggcagtaatt
     7861 tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg
     7921 ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg
     7981 aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg
     8041 cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca
     8101 ttgccgccgc ggaatccgac acgccggaaa aacatattgt ccgcgccacc aactcggtta
     8161 tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt
     8221 ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggttctggaa ttgctcaata
     8281 ttccccatgc gaaattaatc atggactgtg tgatattact ttccgtaacc agttgcctga
     8341 actcggccct gtataccgcc tcaaggatgc tctactcctt aagccgtcgc ggtgatgctc
     8401 ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta cgtggcggtg ttactctcca
     8461 ccggcgcggc attcttaacg gtggtggtga actattacgc gcctgcgaag gtatttaaat
     8521 ttctgatcga cagctccggc gctatcgccc tgctggttta tttagtcatc gccgtttcac
     8581 agttgcggat gcgcaaaatt ctgcgagcgg aaggaagcga aattcgcttg cgaatgtggc
     8641 tttatccctg gctcacctgg ctggtcatcg gctttattac ctttgtgttg gtagtgatgc
     8701 tattccgtcc ggcgcaacag ttagaagtga tctccaccgg cttattagcg atagggatta
     8761 tctgtaccgt gccaattatg gctcgctgga aaaagctggt attgtggcaa aaaacacccg
     8821 ttcataatac gcgctgataa tgatcaggag tcacaccatg accattacgt ctctggatgg
     8881 ctatcgctgg ctgaagaacg atattattcg cggtaatttt caaccggatg aaaaattacg
     8941 gatgagtttg ctgacatcgc gttatgcact tggcgttggg ccgttgcggg aagcactttc
     9001 tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc gggtggcgtc
     9061 tatgtcagag caggagctgc tcgatatttt cgatgcccgc gccaatatgg aagcgatgtt
     9121 agtgagtctg gcgattgccc gcggtggcga tgagtgggag gcagacgttc tcgcaaaagc
     9181 gcatctgctc agtaagctgg aagcctgtga cgccagcgag aaaatgcttg atgagtggga
     9241 tctgcgtcat caggcgtttc atacagcaat tgtggcgggc tgtggttctc actatttgct
     9301 gcaaatgcgt gaacggttgt ttgatctggc ggcgcgctat cgatttatct ggctgcggcg
     9361 aacggtgctt tcggtagaaa tgctggagga taaacacgat cagcaccaga ccctgactgc
     9421 ggcggtactg gcgcgagata ccgcgcgcgc cagtgagtta atgcgccagc atttactgac
     9481 gccaattccc attatccagc aggcgatggc tggcaattaa ctactcttcc ggaatacgca
     9541 acacttgccc cggataaatt ttatccgggc tttttagcat cggtttattc gcttcgaaga
     9601 ttttattgta cagattagcg ttaccgtaga cctgtttgga aatggcactc agagtgtcgc
     9661 cagacttaac ggtataaaac tggctggcag tggctggtgt cgccgttttc acctgatcat
     9721 caacactggc aataccggaa atattcccca ccgcaacaag gattttctcc ttcgcctcct
     9781 gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc aatttgaatg ttcactttat
     9841 cagcatcagg tataccggtt ttgctcagat gctcctgcac cttcttcgcc tgatcgtctt
     9901 tatcgtgctg acctgtaacc gcgtcccaga gtttttctcc ggcatctttc acaaaattga
     9961 acagacccat agctacctca ttcgttaacg gaaacactca gaaagtgtag cagaggaacg
    10021 cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg cgggtttgca cccagaacgc
    10081 gtgaatcaaa ccggggatat acccaagcag tgtcagcaga atattaataa tgaacgccca
    10141 accgaaccct ttaccgagca gcacgccgag cggcggtaga atgatggtga tgacgattct
    10201 ccagaaaccc atatgtactc cctataagaa aattactcat tgtttaaaaa gagattttat
    10261 ctcttaagcg tagtaatttt agcggagcct gccagtttca gcttacgctt ttactctcat
    10321 ttacgctgct gaggctggta tatgttgcat ttttagttta gagcttactt aatttagaaa
    10381 atacttaaat aaatatgact gaactcgcgc aattacaggc cagtgccgaa caggcagcgg
    10441 ccttattgaa agcaatgagc catcctaaac gattgctgat tctgtgcatg cttagcggtt
    10501 cccccggcac cagcgcggga gagctgacgc gcattaccgg actgagtgcc tctgcgacat
    10561 cacagcatct cgctcgtatg cgggacgaag ggcttatcga cagccaacgg gatgcccaac
    10621 gcattctata ttccattaaa aatgaggcgg taaatgccat tatcgccacc ctgaaaaatg
    10681 tctattgtcc gtaaggagtc actatggctt tgacaaccat ttcgccgcat gatgcacaag
    10741 aattaatcgc acgcggcgca aagttaatcg atattcgtga cgctgatgaa tatcttcgtg
    10801 aacatattcc tgaagcagat ctggctccat tatccgtgct ggaacagtca ggtcttccac
    10861 ctaaattacg tcgcgagcaa attatattcc actgccaggc aggtaaacgc accagtaata
    10921 acgccgataa attagcagcg attgccgccc ccgcagaaat atttttactc gaagatggga
    10981 ttgatggctg gaaaagagct ggattgccag tagcggtaaa taaatctcaa cccttgccgt
    11041 taatgcgcca ggtgcagatc gctgcgggtg gtttaatatt aatcggcgtt gtactgggtt
    11101 ataccgtaaa tagcggtttc ttcttattaa gtggctttgt tggtgccggg ttactgtttg
    11161 caggaatcag cggtttttgt ggaatggcaa ggttgttaga taagatgccg tggaaccaac
    11221 gagcttgaga agcgacgccg gacgcgccct agcagcgaca tccggcctca gtaattagat
    11281 caggaaatcg tcgagagatt taccttctgc cagcgcctga gcaattggct tcggtgtacg
    11341 gccctgaccg gtccaggttt tagtttcacc gttaacatcg gtgaatttat atttcgccgg
    11401 acgcggctgg cgttttttac cagcgcgtgg tgcagcagca gagctattac ccaataactc
    11461 ttccgggtta attccgtcag ccttcatcag ctccagccag gtgctaattt tttcctgacg
    11521 ctcagccagt tcacgctgct gctgttcttc ttcttcacgt ctttctttag tgacaaccct
    11581 gaatttttcg agcatttctt caagaacgtc aatggagaat tcgcgagcca tcgcacggag
    11641 ggtgcgaatg ttatttaaac tttgtaacat tacggacata ataaagaaaa ccttttaacg
    11701 ccaaaacaaa aaagtatttc gtcgcgtagt gtaagcgatc acttattata tttccacaaa
    11761 atttaatcta tttacagtct tgcgcgagat tatttcagct gttattttct gaaactctca
    11821 gaataatttc aagattatat attagccatt ttgatgagta atttagatta ttggtgaaca
    11881 gaacggcgct ctctgctcgc taattccgca agcctgacaa aaccgaagtg aataaaaatg
    11941 caatataaca gaataaaaga
//
LOCUS       DAETQN010000126        11877 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.126, whole genome shotgun sequence.
ACCESSION   DAETQN010000126 DAETQN010000000
VERSION     DAETQN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.126"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            486..1157
                     /locus_tag="K0W68_003498"
     CDS             486..1157
                     /locus_tag="K0W68_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBI3074961.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1167..2363
                     /locus_tag="K0W68_003499"
                     /pseudo
     CDS             1167..2363
                     /locus_tag="K0W68_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2363..2923
                     /locus_tag="K0W68_003500"
     CDS             2363..2923
                     /locus_tag="K0W68_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBI3074962.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2925..3698
                     /locus_tag="K0W68_003501"
     CDS             2925..3698
                     /locus_tag="K0W68_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HBI3074963.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3886..4161
                     /gene="frmR"
                     /locus_tag="K0W68_003502"
     CDS             3886..4161
                     /gene="frmR"
                     /locus_tag="K0W68_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HBI3074964.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4196..5305
                     /gene="frmA"
                     /locus_tag="K0W68_003503"
     CDS             4196..5305
                     /gene="frmA"
                     /locus_tag="K0W68_003503"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HBI3074965.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5398..6231
                     /gene="fghA"
                     /locus_tag="K0W68_003504"
     CDS             5398..6231
                     /gene="fghA"
                     /locus_tag="K0W68_003504"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBI3074966.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6457..6996)
                     /locus_tag="K0W68_003505"
     CDS             complement(6457..6996)
                     /locus_tag="K0W68_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HBI3074967.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="K0W68_003506"
     CDS             complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="K0W68_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HBI3074968.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="K0W68_003507"
     CDS             complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="K0W68_003507"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HBI3074969.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="K0W68_003508"
     CDS             complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="K0W68_003508"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HBI3074970.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10553..11362)
                     /gene="mhpD"
                     /locus_tag="K0W68_003509"
     CDS             complement(10553..11362)
                     /gene="mhpD"
                     /locus_tag="K0W68_003509"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HBI3074971.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11372..>11877)
                     /gene="mhpC"
                     /locus_tag="K0W68_003510"
     CDS             complement(11372..>11877)
                     /gene="mhpC"
                     /locus_tag="K0W68_003510"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HBI3074972.1"
                     /translation="AFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQP
                     TIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPR
                     LAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLV
                     LNFLARP"
BASE COUNT         2912 a         3177 c         2908 g         2880 t
ORIGIN      
        1 aatatgcaat tactccgttc taacaattgg gaaaattact acacttcatt ttagtgatgc
       61 ataaacaacc acattacgag atttattact tcattgtttc tatcaataca tataataaaa
      121 ctattatatt cgcattgtat atatcactgt atcattctca atcactcgca tattacattc
      181 acataatgta tataaacaat acttgttcac agaacatatt ttttaaatgc aattaattga
      241 caaactaaat cacatctaaa tacaatgtat aaaacaaaat aactatacat accaaacata
      301 caaacactta acatatgaat tgcataaaaa cattacccag tcgaacctcc gggtctgaaa
      361 gcccttgctt ttcagttatt agcaacattt aacatttata aattaacatc tggctgcgcc
      421 agagattacg tctctgagtg catcgttgtg cttgtagtat aataaataaa atggagtgtc
      481 attaagtgga taaggtttta gattcagccc tcctttcttc ggcaaataaa agaaagggta
      541 tccttgctat tggcgcacat cctgacgata tagaattagg ctgtggcgca tcgcttgctc
      601 gtcttgcgca aaaaggaatt tatatcgcag cagtggtgat gactaccggc aactctggta
      661 cagatggaat aatcgatcgc catgaagaat cgcgcaacgc cctaaagata ttagggtgca
      721 accaaactat tcatcttaat tttgctgaca cccgcgctca tttacagctc aatgatatga
      781 tttccgccct ggaagacatc attaaaaatc aaattccttc tgatgttgaa atcatgcggg
      841 tatataccat gcatgatgcc gaccgccatc aggatcatct cgctgtttat caagcttcaa
      901 tggttgcctg ccgcactatt ccacaaattc tcggctacga aaccccaagt acctggcttt
      961 catttatgcc tcaggttttt gaatccgtta aagaagaata tttcacagtc aaacttgctg
     1021 cattaaaaaa acataaaagc caggaacgac gtgattatat gcgtcatgat cgcctgcgtg
     1081 cagttgcaca atttcgcggg caacaggtca atagcgatct gggtgaaggc tttgtaattc
     1141 ataaaatgat tctttgagca aattgcatga aaacctggat atttatctgt atgtccatag
     1201 caatgttgct atggttttta agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta
     1261 ttgacgccat tatacctgcg tataacgaag gcccgtgtct ggcgcagtca ctggataatc
     1321 tactgcgtaa cccttatttt tgccgggtaa tttgcgttaa cgacggctcc acggacaata
     1381 ccgaagcggt catggcggaa gtcaaacgca aatggggcga ccgctttgtt gccgtcacgt
     1441 aaaaaaatac cggtaaaggt ggtgcgctga tgaatggcct caattatgcc acctgcgacc
     1501 aggttttttt aagtgatgcc gacacctatg ttccgcccga tcaagacgga atgggctata
     1561 tgctggcaga aattaagcgc ggtgctgatg ccgtaggcgg cattccctct actgcgttga
     1621 aaggcgcggg gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga
     1681 agcgcacgct acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa
     1741 tgttccgtac tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc
     1801 ttgatctcac ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca
     1861 tcgtataccc acaggaatgc aacagtccgc gtgaggagtg gcgtcgctgg cggcgttgga
     1921 ttgtgggcta cgcggtctgt atgcgcctgc ataaaaggct tttatttagc cgcttcggta
     1981 tcttcagtat atttcctatg ctgttggttg tgatttatgg cgttgggatt tatctcacta
     2041 cctggtttaa tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc
     2101 ttatctggat cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct
     2161 ggttgttggt gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct
     2221 ggattatcta tggacttatt gcctttttta ctggacgcga acctcagcgc gacaaaccca
     2281 cccgctattc cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa
     2341 ctgaaaaact atctgaagct taatgttaaa gagaaaaatt attcagattg cagaccaggt
     2401 aattattgat gaaactgcag gtgaagttgt tatcggcgcg aatacccgta tttgtcatgg
     2461 tgccgttatt cagggtccgg tagtgattgg cgcaaactgc ctgataggta attatgcgtt
     2521 tattcgtcct ggcacaataa tcagcaatgg tgtaaaaatt ggttttgcca ccgaaattaa
     2581 aaatgcggtt attgaagcgg aagcaacgat tggtccgcaa tgttatattg ccgactcggt
     2641 agttgcaaac caggcatatt tgggcgcaca agtacgtacc agtaatcatc gtctggatga
     2701 acagcctgtg tctgttcgaa ctccagaggg aattatcgct accggatgcg ataaattagg
     2761 ttgttatatc gggcagcgtt cacgccttgg cgtacaagtt attattttgc ctgggcgaat
     2821 tatttccccg aacacgcaac ttggcccgcg cgtgattgta gaacgtaatt tacctaccgg
     2881 aacttattca ctccgacaag aacttatccg tacaggagat taaaatgata aaacggacgt
     2941 tattagcggc ggccattttt agcgcattgc ccgcttatgc cgggttaact tccattaccg
     3001 cgggctacga ttttaccgat tattctggcg atcatggcaa ccgtaattta gcgtatgctg
     3061 aactggtggc gaaagttgaa aacgcaacgc tgctttttaa tctttcccag gggcgtcgtg
     3121 attatgaaac tgaacatttc aatgccactc gcggtcaggg tgcggtctgg tataaatgga
     3181 ataactggct gacaacccga acgggtattg cctttgcgga taatacgccg gtctttgccc
     3241 gccaggattt tcgtcaggat attaacctgg ccctgttgcc aaaaacgctt ttcacgaccg
     3301 gttatcgcta cactaaatat tacgatgatg tcgaagtcga tgcctggcaa ggcggcgtat
     3361 cactctatac tggcccggtg atcaccagct accgctatac ccattatgac tccagcgatg
     3421 caggtggtag ttatagcaat atgatttccg tgcgtctgaa tgacccgcgc ggcactggtt
     3481 atacgcaact atggctaagc cgcggaacag gcgcttacac ctatgactgg acgccagaaa
     3541 cacgctacgg cagcatgaag agcgtcagtt tgcaacgtat tcaaccgctg actgagcaac
     3601 ttaatctcgg gctgacggca ggtaaagtgt ggtacgacac cccaaccgat gattataacg
     3661 gtctgcaact tgcagcccat ctgacctgga aattctgatt gcttctgccg cccgctatcc
     3721 ggggcggcct tccctgccga ttagccccac tccctttcct cgttgttttc cgaccacatt
     3781 caccggataa attttattct ccagcgttat atactatagg gaggtatgca ttgacatata
     3841 gcatacctcc ctatagtata ttgcgtgcag atgatgaggt gcgaaatgcc cagtactccg
     3901 gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc gggggcagat tgatgctctg
     3961 gaacggtcgc tggagggtga tgccgaatgc cgtgccatac tccaacagat cgctgccgtt
     4021 cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa gccatatccg ggaaacgttt
     4081 gaccgaaatg actgctacag ccgcgaagtc agccaatccg ttgacgacac tattgaactg
     4141 gttcgagcct atcttaaata gctgaatcta ttaccatatt gaggaagagc gagagatgaa
     4201 atcacgtgct gccgttgcat ttgctcccgg taaaccgctg gaaatcgttg aaattgacgt
     4261 tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc cataccggcg tttgccatac
     4321 cgacgcattt accctctccg gggatgaccc ggaaggtgta ttcccggtgg ttctcggtca
     4381 cgaaggggcc ggcgttgtgg ttgaagtcgg tgaaggcgta accagcgtca aacctggcga
     4441 ccatgtgatc ccgctttaca ccgcggagtg cggcgagtgt gagttctgtc gttctggcaa
     4501 aactaacctc tgtgttgcgg ttcgcgaaac ccagggtaaa ggcttgatgc cagacggcac
     4561 cacccgtttt tcttacaacg ggcagccgct ttatcactac atggggtgct ctacattcag
     4621 tgaatacacc gtggtcgcgg aagtgtctct ggccaaaatt aatccagaag caaaccatga
     4681 acacgtctgc ctgctgggct gtggcgtgac caccggtatt ggcgcggtgc acaacacagc
     4741 taaagtccag ccaggtgatt ctgttgccgt gtttggtctt ggcgcgattg gtctggcagt
     4801 ggttcagggc gcgcgtcagg cgaaagcggg acggattatc gctatcgata ccaacccgaa
     4861 gaaattcgat ctggcgcgtc gcttcggtgc taccgactgc attaacccga atgactacga
     4921 caaaccgatt aaagatgtcc tgctggatat caacaaatgg ggtatcgacc atacctttga
     4981 atgcatcggt aacgtcaacg tgatgcgtgc ggcgctggaa agtgcgcacc gcggctgggg
     5041 tcagtcggtg atcatcgggg tggcaggttc tggtcaggaa atctccaccc gtccattcca
     5101 gttggtcact ggtcgcgtat ggaaaggttc cgcgtttggc ggcgtgaaag gtcgttccca
     5161 gttaccgggt atggttgaag atgcgatgaa aggtgatatc gatctggaac cgtttgtcac
     5221 gcataccatg agccttgatg aaattaatga cgccttcgac ctgatgcatg aaggcaaatc
     5281 cattcgaacc gtaattcgtt actgatttcc cgcaggtata ccccgtccac ttcagacggg
     5341 gttcttaata ctctccccgg gcagccgtcc gggggattaa ccgtgagata atgactgatg
     5401 gaactcattg aaaaacatgc cagctttggc ggctggcaaa atgtgtatcg gcattattcc
     5461 caatcactga aatgtgaaat gaatgtcggc gtctatctcc caccaaaagc cgcgaatgaa
     5521 aaattgccgg tgctgtactg gctttcaggc ctgacctgca acgagcagaa tttcattact
     5581 aaatcgggga tgcagcgtta cgcggctgag cacaacatta ttgttgttgc gccggacacc
     5641 agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg atctcgggca aggtgccggg
     5701 ttttacctga acgcaacgca agcgccgtgg aatgaacatt acaaaatgta tgactatatc
     5761 cgcaacgagc tgccggattt agtgatgcaa cattttccgg caacgaccag aaagtctatc
     5821 tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg cgttacgtaa cccagatgaa
     5881 tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat cgcaagtacc gtggggacag
     5941 caagcctttg ccgcatatct tggtgaaaat aaagatgcct ggttggatta cgatccggtg
     6001 agtcttattt cacaaggtca acgcgttgcg gaaatcatgg ttgatcaggg gttgagtgat
     6061 gatttttacg cggaacagct gcggactcca aatcttgaaa agatctgcca ggagatgaat
     6121 atcaagacgt taatccgtta tcacgagggt tacgaccaca gctattattt tgtctccagt
     6181 tttattggcg agcatattgc ctaccacgcc aataaactga atatgcgttg ataatagtgc
     6241 acgactgccg gatgcggcgt gaacgcctta tccggcctac acttcgcccg taaaccgtag
     6301 gcctgataag acgcgcaaag cgtcgcatca ggcatggcgc acgactgccg gatgcggcgt
     6361 gaacgcctta tccggtctac acttcgccca taaaccgtag gcctgataag acgcgcatag
     6421 cgtcgcatca ggcatctgcg cacgacttat ctatgtttac cacatcaaat catcaggcac
     6481 tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg gcactgagcg cgctgtgcaa
     6541 cacaatactg ctggcatcac gctgagcaat tttatcggca acactggcgg gaataatggt
     6601 gtattcacct tcgctattgt tatcgaccaa caagcgggca atcgccagac gaccattaat
     6661 caactgcgcc tgtgtgagct tatcgacaaa aatcttctta atcagattgc cgtcagtgaa
     6721 gttaaaacca atatcgccat tggcaatggt gattcggttc atttcaatga gctgcttcac
     6781 ctgggcttta tattctttcg ccaacgccgc ttgtttttgc tgttcgctaa gctgtttatc
     6841 acgctcaagc tgtgcctttt tattttcttc taccgcagcc cgagcttcac gcgcctgaac
     6901 gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt ttgctggtga ctaatcctgc
     6961 tttgagcaac tgctcttgta aggtaagttt tgccatcttt gtttccaaac ccgctgaata
     7021 atttttagcg agtatacctg taaacgccgt gggtgtattg ggggcaagtc cctttcgccc
     7081 ctgcttaata aggcgcgtca ggcatcggca cacggctgca ttcttgatct ccgactcatc
     7141 aaaataaaca ccgccaaccc agcaacaaga atacccggtg cagaagcggc cattacgccg
     7201 accgtgccag tgcctaatgc cagcattttc ccggccagta acggaccgct catagccccc
     7261 agacgcccta ccgccacggc tgttcccaca cctgttgcgc ggatctgcga actgtaaaac
     7321 aacagtgcca gggcatacaa aacgctttgc ccacctgtcg caaacaaccc cgcgacaaat
     7381 cccgccagca acataccgtt aaatgacgac accgttccaa gcgccagcag cgaagctaac
     7441 atgccgctat aaatcagtag cgacatggtt actggacgca gcttatccat caatgcgccc
     7501 aacattaacg tcccgcttgc cgcccccatt tgcagggcaa acatcacccc tgccgcctgc
     7561 gatggctgga atccttgctc caccaaaagt agcggtagcc agttgatcaa catataaacc
     7621 accagcagag tgaagaaata acacaaccac agcagcagcg tcgcggttgc cgtttctggc
     7681 gcaaataagg cacgcagtgg tggcgcagcc tgtttttcgc cagcgaaaac cgctgactcc
     7741 ggcagccagc gcattaatag cggcaccaga atcaacggca ccacaccacc tacccaaaac
     7801 accgtttgcc atgctaagtt tgcccccgcg aaacccagtg tcgccgccag cgccgcgcca
     7861 atgggaacac cgcaatacat caggctcact gccgtcccac gaaaacgtgg acccgcggct
     7921 tcagatgtca gggcgataag attcggcaac gccgccccca gcccgacacc ggtcatcagc
     7981 cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg ttgccagtga gaacaaacca
     8041 aacagcgcaa ctgagccaat caaaatacgc ttacgaccat aacggtctgc cagcatcccg
     8101 ccaaccaacg cgccgggtag caaaccgagt attccggcgc taaatatcca gcccatttgc
     8161 attttatcga gtgcgaaagc ctgggcgatg ccacccgccg caatgccagc cgcctgaaga
     8221 tccagccctt ccatcagagc gaccaaaaaa caaagcccga tggtcagcat caggcgggat
     8281 gaagatgatg aaggggtacg agtcgacata agatgaacct cattgcgagt gcaggtgaat
     8341 cgcgactgat acattgccag ccgcgtaatc agaactattt aatgactgcc tacgggcgaa
     8401 atgtagcgaa atgtaggccg gataaggcgt tcacgccgca tccggcagtc gtgcgcagat
     8461 gcctgatgcg acgctaacgc gtcttatcag acctacaaat tctcacgcca tccgtaggcc
     8521 ggacaaggcg ttcacgccgc atccggctgt cgtgctggga tgggcataca aaatgcacgt
     8581 tatcggcagt catcattatt tgttgttgcg cagatccagc gccacgtcga cgatcatatc
     8641 ctcctggccg ccaaccatcc ggcgtttgcc cagctcaacg agaatatcca ccgcacttaa
     8701 gccataacgc gccgccgccg tttcacagtg acgcaggaag ctcgagtaaa caccagcgta
     8761 tcccagcgcc agcgtttcgc gatcgactcg taccggacga tcctgcaacg gacgcaccag
     8821 gtcgtcggcg gcatccatta acgcatagag atcggtccca tgctgccagc ccagtttatc
     8881 cgcggcggca ataaacactt ccagcggtgc gttacctgcg cccgcgccca ttcccgcgag
     8941 gctggcgtcg attcggtcgc agccctcttc caccgccgcg atagagttcg ccacgccaag
     9001 actcaggtta tggtgagcgt gcatgccggt ttgcgtttct ggtttcaaca ctgctttcag
     9061 ggcgcggaaa cggtcacgga tatcgctcat gttcatcgca ccgccagaat ccaccacata
     9121 aatacaggtc gcaccgtagc cttccatcag ctttgcctgc ttggcgagat tctccggcgt
     9181 ggtcatatgg ctcatcatca gaaaaccaac ggtgtccatt ccgagctcgc gggcatactg
     9241 aatatgctgg gcggaaacat cagcttcggt acagtgcgtt gccacacgaa ccacccgcgc
     9301 gccagcctgc caggcatttt tcagatcgtg aatagtgccg attcctggca gcaacaacgt
     9361 cgcgattttg gcgtgcctca ccacatccgc cgccgcttca atccattcaa ggtcgctatg
     9421 tgcgccgaaa ccatagttaa agctggaacc ttgcaaaccg tcgccgtggg ccacttcaat
     9481 cgaatccacg cgggcatcgt ccagtgcttt ggcaatctgg cgaacgtttt ccagcgaata
     9541 ctgatgacga atggcgtgca taccgtcacg caatgtgacg tccgagatat aaagtttttt
     9601 accgttcatg ccgcttctcc tgccttgcgc gccagtgact gggccatttt ttccgctgtc
     9661 gccagcgcac tggaagtcat aatatcgagg ttgcccgcat aggcaggcag ataatgcgct
     9721 gcgccttcga cttccagcca gaccgctgtt ttcagtccgg agaattgccc cacgcccggt
     9781 aaattgaccg gtttatcctg cgggataact tcaaactgca cgcgctgttt caggcgataa
     9841 cccggtacgt aagcctgcac cgcctcagcc atttcattga ttgaggcttc gatatcatct
     9901 tgtgaagctt cgtcgctcaa tacatacacc gtgtcacgca tcatcaacgg tggctctgct
     9961 gggttaagca caataatcgc cttccctttt gccgcgccgc ccaccacttc aatggctcgg
    10021 gaagtggttt ccgtaaattc atcgatattg gcacgcgtgc caggtccggc agatttactg
    10081 gcgatagaag cgataatttc ggcgtaatga acacgcgcca cgcgtgaaac tgccgccacc
    10141 attggaatgg tggcctggcc gccgcaggtg accatgttga cgttcagttg atcgacgttc
    10201 gcctcgaggt taaccaccgg cacgcagtaa gggccgatgg cagcaggcgt caggtcaatt
    10261 aagcgaatat ccggtttcgc ttcgcgtaaa gcggcatcgt ttttcacatg cgcgcccgcg
    10321 ctggtcgcat caaatacaat gtcgatatca gcaaattcag gcatgttcat cagcccgatc
    10381 accccttcat gggtggtggc gacgcccata cgtctggcac gcgccagacc gtcggactga
    10441 ggatcaatgc cgaccatcac cgccatctcc agatgctgac cgtgacgcaa aattttaatc
    10501 atcagatcgg tgccaatgtt gccagaaccg ataatggcga ctttacgctt actcatgaca
    10561 gacttccttt tggggctgcg cttgaaaatg tcgcagcaac tgaacctatg ccttcaatat
    10621 gggcttcaaa acgatcgccc gcattcaccg ccaccatcgg gcctaatgcc ccggtaagaa
    10681 tgatatctcc ggcgcgcagc ggttcaccca gactggccat tttgcgtgcc agccagacgg
    10741 cggcattaag cggatgtccc aggcattcgc tgccgcgccc gctggaaacc tcttcgttat
    10801 tacgcgtcat cttcatggcg cagtttttca ggtctaaccc cgccggacgt tgcgccggac
    10861 cgccgatgac atacaccccg caggaagcgt tatctgccac ggtatcgaca aactgaatcg
    10921 accagtcgcg aatgcggctc cccaccactt ccagcgccgg aagtacccat tcaatggcgt
    10981 tatacagttc gtcgaaggtg atatcagttg cgggtaaatc gcggttcaac accagtgcga
    11041 tctccgcttc aatgcggggt tgcagaacac gggaaaaagg aatgatttcg ttatcgccat
    11101 aacacatgtc ggcaaataac gtcccaaaat ccggttgatc aacgcccagt tgttgttgca
    11161 ctttcggatg cgtcaggccc actttacgcc ctaccacgcg acgcccctgc acaacgtcat
    11221 attgcacatt tatgtgctga atggcgtagg ccgcttcagc gtgatcgata ccaatcagat
    11281 cgcgcagcgg cgcaatcgct tcgccctgct ctgcggcgcg gcgtaaatcc gccgccagtt
    11341 gctcaagagt atgcttcgtc atgaccattc cttaagggcg tgcgaggaaa ttcagcacca
    11401 attgattgaa agcgtcggca tgttcccact gcgcccagtg accacagtcg cggaagatat
    11461 gcagttcaga accggcaatg ccggacagca gacgcagacc cgcatccatc ggcacaaagc
    11521 ggtcgttgcg cccccagaca atcagggttt gcgctttgat ttccgccaga cgtgggccaa
    11581 aatccgggaa ctgtttcgga ttagcttcca ggctcttaac gaagttttcc aggtgatcgc
    11641 ggcgcgacag catattattc aggcgcgctt caaacagggc gtcggtcaaa tcgctggtat
    11701 cgaaaacgaa gatatccatc atcagcttca ggttttcgat agtcggctga cgataaagct
    11761 gattcagtcg cttaatacct tcggttggca tcggcgtaaa caaactcatg ccgcccgtac
    11821 cgccgcccat cagcaccagt ttgccgacgc gctccggcca gttaagggtg aacgcca
//
LOCUS       DAETQN010000127        11816 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.127, whole genome shotgun sequence.
ACCESSION   DAETQN010000127 DAETQN010000000
VERSION     DAETQN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.127"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..389
                     /locus_tag="K0W68_003511"
     CDS             <1..389
                     /locus_tag="K0W68_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBI3074973.1"
                     /translation="SSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALL
                     KREGRCPSDVEHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRAL
                     RKGQASAFYYGDPLGEMRLVSRVFEM"
     gene            complement(588..938)
                     /locus_tag="K0W68_003512"
     CDS             complement(588..938)
                     /locus_tag="K0W68_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBI3074974.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(1141..2154)
                     /gene="intI1"
                     /locus_tag="K0W68_003513"
     CDS             complement(1141..2154)
                     /gene="intI1"
                     /locus_tag="K0W68_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBI3074975.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2312..2785
                     /locus_tag="K0W68_003514"
     CDS             2312..2785
                     /locus_tag="K0W68_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HBI3074976.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            2916..3704
                     /locus_tag="K0W68_003515"
     CDS             2916..3704
                     /locus_tag="K0W68_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HBI3074977.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            3910..4257
                     /locus_tag="K0W68_003516"
     CDS             3910..4257
                     /locus_tag="K0W68_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HBI3074978.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            4251..5090
                     /gene="sul1"
                     /locus_tag="K0W68_003517"
     CDS             4251..5090
                     /gene="sul1"
                     /locus_tag="K0W68_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HBI3074979.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            5218..5421
                     /locus_tag="K0W68_003518"
     CDS             5218..5421
                     /locus_tag="K0W68_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3074980.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            5577..6782
                     /gene="chrA"
                     /locus_tag="K0W68_003519"
     CDS             5577..6782
                     /gene="chrA"
                     /locus_tag="K0W68_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HBI3074981.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            6793..7098
                     /locus_tag="K0W68_003520"
     CDS             6793..7098
                     /locus_tag="K0W68_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3074982.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            7142..7207
                     /locus_tag="K0W68_003521"
                     /pseudo
     CDS             7142..7207
                     /locus_tag="K0W68_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            7325..8089
                     /locus_tag="K0W68_003522"
     CDS             7325..8089
                     /locus_tag="K0W68_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HBI3074983.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(8582..9166)
                     /locus_tag="K0W68_003523"
     CDS             complement(8582..9166)
                     /locus_tag="K0W68_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBI3074984.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(9166..10404)
                     /locus_tag="K0W68_003524"
     CDS             complement(9166..10404)
                     /locus_tag="K0W68_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3074985.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(10401..11306)
                     /locus_tag="K0W68_003525"
     CDS             complement(10401..11306)
                     /locus_tag="K0W68_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBI3074986.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            complement(11428..>11816)
                     /locus_tag="K0W68_003526"
     CDS             complement(11428..>11816)
                     /locus_tag="K0W68_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBI3074987.1"
                     /translation="SSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALL
                     KREGRCPSDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRAL
                     RKGQASAFYYGDPLGEMRLVSRVFEM"
BASE COUNT         2490 a         3391 c         3450 g         2485 t
ORIGIN      
        1 tctcctcccg tcgtaacagc aaagctgcat accggtttct gggtaaaatc ctcaacaacg
       61 tgaagaagtg gcagatccca cgattcatca acacggataa agcgcccgcc tatggtcgcg
      121 cgcttgctct gctcaaacgc gaaggccggt gcccgtctga cgttgaacac cgacagatta
      181 agtaccggaa caacgtgatt gaatgcgatc atggcaaact gaaacggata atcaacgcca
      241 cgctgggatt taaatccatg aagacggctt acgccaccat caaaggtatt gaggtgatgc
      301 gtgcactacg caaaggccag gcctcagcat tttattatgg tgatcccctg ggcgaaatgc
      361 gcctggtaag cagagttttt gaaatgtaag gcctttgaat aagacaaaag gctgcctcat
      421 cgctaacttt gcaacagtgc ccccaatgcg ctgtccagtc atggaattcc ctgcaaaatg
      481 tcagggaaga ctctatgacc ttcaacgaga tatgtcaata aattcaaaat tcaatcctat
      541 cctgacgcaa tttacacatg gcatctgaca tcaggttagg gtatgcctca acctgacggc
      601 ggcgaatcac aagcgtccgg tttgacgctg gtttcggtcg cagtgctggc ccgcgcctgg
      661 aagcgctgat agcactccag cccgcagaaa tgctccacgt actcggcccc ttccggcgtg
      721 aaggcggcat cgagcgggat ttccttgcag cacacgcagc aactggtgct cggttcattg
      781 gcgttcatgg tggtgttcct ccattggttg acgaagccga cgaaggccgc cgccggcatc
      841 ggcctggcga acaggaaacc ctgcaccgtg tcgcaacccg cctgccgcaa ccacgcaagg
      901 cagtcgggtg tttccacacc ctcggctacc acctccattg tcgttttcag aagacggctg
      961 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatcc atcaggcaac
     1021 gacgggctgc tgccggccat cagcggacgc agggaggact ttccgcaacc ggccgttcga
     1081 tgcggcaccg atggccttcg cgcaggggta gtgaatccgc caggattgac ttgcgctgcc
     1141 ctacctctca ctagtgaggg gcggcagcgc atcaagcggt gagcgcactc cggcaccgcc
     1201 aactttcagc acatgcgtgt aaatcatcgt cgtagagacg tcggaatggc cgagcagatc
     1261 ctgcacggtt cgaatgtcgt aaccgctgcg gagcaaggcc gtcgcgaacg agtggcggag
     1321 ggtgtgcggt gtggcgggct tcgtgatgcc tgcttgttct acggcacgtt tgaaggcgcg
     1381 ctgaaaggtc tggtcataca tgtgatggcg acgcacgaca ccgctccgtg gatcggtcga
     1441 atgcgtgtgc tgcgcaaaaa cccagaacca cggccaggaa tgcccggcgc gcggatactt
     1501 ccgctcaagg gcgtcgggaa gcgcaacgcc gctgcggccc tcggcctggt ccttcagcca
     1561 ccatgcccgt gcacgcgaca gctgctcgcg caggctgggt gccaagctct cgggtaacat
     1621 caaggcccga tccttggagc ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc
     1681 cagatccttg acccgcagtt gcaaaccctc actgatccgc atgcccgttc catacagaag
     1741 ctgggcgaac aaacgatgct cgccttccag aaaaccgagg atgcgaacca cttcatccgg
     1801 ggtcagcacc accggcaagc gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg
     1861 cagatccgtg cacagcacct tgccgtagaa gaacagcaag gccgccaatg cctgacgatg
     1921 cgtggagacc gaaaccttgc gctcgttcgc cagccaggac agaaatgcct cgacttcgct
     1981 gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     2041 gacataagcc tgttcggttc gtaaactgta atgcaagtag cgtatgcgct cacgcaactg
     2101 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     2161 ttatgactgt ttttttgtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     2221 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     2281 taaaacaaag ttagccatta agggagttaa attgaaaata tcattgattt ctgcagtgtc
     2341 agaaaatggc gtaatcggta gtggtcctga tatcccgtgg tcagtaaaag gtgagcaact
     2401 actctttaaa gcgctcacat ataatcaatg gctccttgtc ggaagaaaaa catttgactc
     2461 tatgggtgtt cttccaaatc gcaaatatgc agtagtgtca aagaacggaa tttcaagctc
     2521 aaatgaaaac gtcctagttt ttccttcaat agaaaatgct ttgaaagagc tatcaaaagt
     2581 tacagatcat gtatatgtct ctggcggggg tcaaatctat aatagcctta ttgaaaaagc
     2641 agatataatt catttgtcta ctgttcacgt tgaagtcgaa ggtgatatca aattccctat
     2701 aatgcctgag aatttcaatt tggtttttga acagtttttt atgtctaata taaattatac
     2761 ataccagatt tggaaaaaag gctaacaatg cgttgcagca ccagtcgctt cgctccttgg
     2821 acagctttta agtcgcgtct ttgtggtttt gctgcgcaaa agtattccac aaagccgcaa
     2881 cttaaaagct gccgctgaac ttaacgttag gcatcatggg tgaatttttc cctgcacaag
     2941 ttttcaagca gctgtcccac gctcgcgcgg tgatcgagcg ccatctggct gcgacactgg
     3001 acacaatcca cctgttcgga tctgcgatcg atggagggct gaagccggac agcgacatag
     3061 acttgctcgt gaccgtcagc gccgcaccta acgattcgct ccggcaggcg ctaatgctcg
     3121 atttgctgaa agtctcatca ccgccaggcg atggcggaac atggcgaccg ctggagctaa
     3181 ctgttgtcgc tcgaagcgaa gtagtgcctt ggcgctatcc ggcgcggcgt gagcttcagt
     3241 tcggtgagtg gctccgccac gacatccttt ccggaacgtt cgagcctgcc gttctggatc
     3301 acgatcttgc gattttgctg accaaggcga ggcaacacag ccttgcgctt ctaggcccat
     3361 ccgcagccac gtttttcgag ccggtgccga aggagcattt ctccaaggcg cttttcgaca
     3421 ctattgccca gtggaatgca gagtcggatt ggaagggtga cgagcggaac gtcgttcttg
     3481 ctcttgctcg catttggtac agcgcttcaa ctggtctcat tgctcctaag gacgttgctg
     3541 ccgcatgggt atcggagcgt ttgcctgccg agcatcggcc cctcatctgc aaggcacgcg
     3601 cggcgtacct gggtagcgag gacgacgacc tagcaatgcg cgtcgaagag acggccgcgt
     3661 tcgttcgata tgccaaagca acgattgaga gaatcttgcg ttgagcggca tgtgcgaaaa
     3721 gtgcatcgac ccgcgccgag ggcatctgat gcctaactcg gcgttcaagc ggacgggctg
     3781 cgcccgccgc tcaactatgc gttagatgca ctaagcacat aattgctcac agccaaacta
     3841 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     3901 agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca
     3961 acatccgcat taaaatctag cgagggcttt actaagcttg ccccttccgc cgttgtcata
     4021 atcggttatg gcatcgcatt ttattttctt tctctggttc tgaaatccat ccctgtcggt
     4081 gttgcttatg cagtctggtc gggactcggc gtcgtcataa ttacagccat tgcctggttg
     4141 cttcatgggc aaaagcttga tgcgtggggc tttgtaggta tggggctcat aattgctgcc
     4201 tttttgctcg cccgatcccc atcgtggaag tcgctgcgga ggccgacgcc atggtgacgg
     4261 tgttcggcat tctgaatctc accgaggact ccttcttcga tgagagccgg cggctagacc
     4321 ccgccggcgc tgtcaccgcg gcgatcgaaa tgctgcgagt cggatcagac gtcgtggatg
     4381 tcggaccggc cgccagccat ccggacgcga ggcctgtatc gccggccgat gagatcagac
     4441 gtattgcgcc gctcttagac gccctgtccg atcagatgca ccgtgtttca atcgacagct
     4501 tccaaccgga aacccagcgc tatgcgctca agcgcggcgt gggctacctg aacgatatcc
     4561 aaggatttcc tgaccctgcg ctctatcccg atattgctga ggcggactgc aggctggtgg
     4621 ttatgcactc agcgcagcgg gatggcatcg ccacccgcac cggtcacctt cgacccgaag
     4681 acgcgctcga cgagattgtg cggttcttcg aggcgcgggt ttccgccttg cgacggagcg
     4741 gggtcgctgc cgaccggctc atcctcgatc cggggatggg atttttcttg agccccgcac
     4801 cggaaacatc gctgcacgtg ctgtcgaacc ttcaaaagct gaagtcggcg ttggggcttc
     4861 cgctattggt ctcggtgtcg cggaaatcct tcttgggcgc caccgttggc cttcctgtaa
     4921 aggatctggg tccagcgagc cttgcggcgg aacttcacgc gatcggcaat ggcgctgact
     4981 acgtccgcac ccacgcgcct ggagatctgc gaagcgcaat caccttctcg gaaaccctcg
     5041 cgaaatttcg cagtcgcgac gccagagacc gagggttaga tcatgcctag cattcacctt
     5101 ccggccgccc gctagcggac cctggtcagg ttccgcgaag gtgggcgcag acatgctggg
     5161 ctcgtcagga tcaaactgca ctatgaggcg gcggttcata ccgcgccagg ggagcgaatg
     5221 gacagcgagg agcctccgaa cgttcgggtc gcctgctcgg gtgatatcga cgaggttgtg
     5281 cggctgatgc acgacgctgc ggcgtggatg tccgccaagg gaacgcccgc ctgggacgtc
     5341 gcgcggatcg accggacatt cgcggagacc ttcgtcctga gatccgagct cctagggatc
     5401 gcctcagaaa acggaaaata aagcacgcta agccgtaagt aagcgtgctc ctgtgaaagc
     5461 cacagctaaa actgcgtagt acacatagag gtattttttt ctagatttat atcgaatttc
     5521 gttatcgaat ttcgcttatt attgatcaac tcgttgcaga tcatgaaggc gaaagtatga
     5581 cgaataaccc taccgatgac agcagaccat ggtcggtctt tcttattttt ctgcggcttg
     5641 gattgacatc ttttggcggc cccattgcgc acttgggcta cttccgcgcc gaatttgtca
     5701 cacggcggcg ctggctctcc gaacggagct atgctgactt ggtcgcgctt tgtcagttct
     5761 tgccagggcc tgcaagcagc caggtcggca tagcggtagg actgtctcgg gctggataca
     5821 gcggggcgct ggctgcttgg gctggcttca cgctgccgtc tgccatagcc ttgatccttt
     5881 ttgcgctcgg catctccagc tatggcgatt acgtctcgca gggcgcgttg catggcttaa
     5941 aagtggtggc tgtggccgtg gtcgctcaag cagtatgggg catggcgcgt aacctatgca
     6001 cggatgggct gcgagtcacc atcatggcaa ttgctacctg cgtcgtttta cttgtgccgt
     6061 ccgcgtgggg acaggttggc gtgattgcta tcgcaggcat cgcaggccgg ttattgttca
     6121 agccagcgaa agttgttgag catgaccccc tacctatcac ggtcagtcac cgggccggcg
     6181 tgctttggct ctcgctgttc tttgtcttgc tgattggcct gccggtgttg gccgaactga
     6241 tgccaagtca aaccatggca atggtggatt ccttctatcg tgtcggatca ctggtgttcg
     6301 gcggtggtca cgttgtgctg ccattactgc aagccgaagt ggtgccctcc ggctgggtca
     6361 acaatgaatc ctttctcgcg gggtacgggg cagctcaagc ggtgcccggc cctttgttca
     6421 cgttcgccgc gtttcttggt gcctcgatga acaccgcccc gtcgggctgg atcggcggca
     6481 ttgtgtgtct gctggctatc ttcgcgccct cgttcttgct ggtcgtcgga tcaatgccat
     6541 tttgggagcg tttgcgccgc aatacaggca tccaagctgc gctggccggg atcaatgccg
     6601 ctgtagtcgg cttgctgctg gccgcgctgt atcagcctgt atggactagc gccatctttc
     6661 agccgcaaga cttcggcttg gcattagttg cccttgtcgc acttatgttc tggaagctcc
     6721 cgccgtggct ggttgtcctc ggtagcggtg cagctgggtg gctgttgagc gtcgctctgt
     6781 gaggcacaaa aaatgactga caaagacctc tacggcggtt tgatccgcct gcacatcctt
     6841 caccatgcag ccgaggaacc tgtctttggg ctggggatca tcgaagagct acgccgacac
     6901 ggctacgaga tgagcgctgg caccgtgtac ccgatgctgc acggcctgga aaaaaaaggc
     6961 tatctgacct cacgccacga acgcaccggg cgacgtgaac ggcgtgttta tgacatcact
     7021 gaacaaggca gaactgcact tgctgatgcg aaaacaaagg tcaaggagct gttcggagag
     7081 ttggtagaag gtggttgagt ttttgcttct atgtcaggtt gagctatacc ctaactggat
     7141 tgtcattttc agaagacgac tgcaccagtt gattgggcgt aatggctgtt gtgcagccag
     7201 ctcctgacag ttcaatatca gaagtgatct gcaccaatct cgactatgct caatactcgt
     7261 gtgggctctg ttgcaaaaat cgtgaagctt gagcatgctt ggcggagatt ggacggacgg
     7321 aacgatgacg gatttcaagt ggcgccattt ccagggtgat gtgatcctgt gggcggtgcg
     7381 ctggtattgt cgctatccga tcagctatcg cgaccttgag gaaatgctgg cggaacgcgg
     7441 catttcggtc gaccatacga cgatctatcg ctgggtccag tgctacgccc cggagatgga
     7501 gaagcggctg cgctggttct ggcggcgtgg ctttgatccg agctggcgcc tggatgaaac
     7561 ctacgtcaag gtgcggggca agtggaccta cctgtaccgg gcagtcgaca agcggggcga
     7621 cacgatcgat ttctacctgt cgccgacccg cagcgccaag gcagcgaagc ggttcctggg
     7681 caaggccctg cgaggcctga agcactggga aaagcctgcc acgctcaata ccgacaaagc
     7741 gccgagctat ggtgcagcga tcaccgaatt gaagcgcgaa ggaaagctgg accgggagac
     7801 ggcccaccgg caggtgaagt atctcaataa cgtgatcgag gccgatcacg gaaagctcaa
     7861 gatactgatc aagccggtgc gcggtttcaa atcgatcccc acggcctatg ccacgatcaa
     7921 gggattcgaa gtcatgcgag ccctgcgcaa aggacaggct cgcccctggt gcctgcagcc
     7981 cggcatcagg ggcgaggtgc gccttgtgga gagagctttt ggcattgggc cctcggcgct
     8041 gacggaggcc atgggcatgc tcaaccacca tttcgcagca gccgcctgat cggcgcagag
     8101 cgacagccta cctctgactg ccgccaatct ttgcaacaga gcccgccgtg ctagtctgct
     8161 cggtgatggt ggagtgaagc caacccgcaa tcgggttatg aatctgcatc gcgattcgca
     8221 atcagctgtc tcttgagcat gtcgaactcc tgcgatgtta tctcgccgtg ctcgtgctgc
     8281 tacaccaact tgtccaactc atccgccaca ccgctcccca ccacctgcga tggccaggtt
     8341 gtttgctggg cgcattggcg gagtatcgtc tcgacccggg acacccacac cggcacaacc
     8401 ttacgggagt gattcactgt caaagaatcg gcccggtgct ctgacgcaag tatcgggatg
     8461 gtcaccattt gtaagccgta gacctgagtg gtgatcaaga cttcgatacc accgaccgta
     8521 ccggtactaa tcgacgacgg tcgtgttcgt cgcctgccgc agggactctg cacacctccg
     8581 tttacgcatg tgcctggagg agttggaaat cgtcgtgttc gggaaacatt aaacacagga
     8641 tggcagcgat ctgagccagc acatgatcag ctagctcacc atccggatcg acggcccact
     8701 gcatcgtcgc gccagcgatg accgagtgca ggagcaactc agctgccgca ggagcacctg
     8761 ggggcagtcg cttgcggatc ccctccacca ccgcgcggtt ccgctggatc gcaagcgtgc
     8821 gtagctccgg cacctggagc tcgtaccagg agatgagata gttcaccgag aagtcgttgc
     8881 gagtgttcat gctccgaacg agcacctgca aaaattccca gagcccttgc ggccctgcgc
     8941 ctatcggtat cgcattcagg taatgccgca cctgctcgac gccgcgctcc atcatcctca
     9001 ccagcagcgt atcgcggttg gtgaagcgct ggattaacgc tgcgcgggag agccccacct
     9061 cctttgctac tccgctgagc gtgaactcta tgggaccgca acgcttcagc actacggtgg
     9121 cggcctcgag tacctcgtca tcggacttga gcttggggcg gggcatcagt gttcaccttc
     9181 tgtatgggtt ggggcggagg ctgtggctgc cgccgccatt gtagcaaatt gaagacggag
     9241 cgagagtaga gccacgagcc ccgcaaacac ggccgataca acgaggccag ggagcggacc
     9301 agcaaggtcg acaaacgcgc cggccgcaag cataaccatg ggcgaggctg acagcatcac
     9361 cgccgagacc gtgccgagta cccggccgag aagttctggc ggcgtgcggt tgtagatggc
     9421 agcgttgaga atgggagaga ctgagccggt cagcagtccc acgagcgcgc ccaacaacat
     9481 cagcaccggc acgcctggca actgtgaaag cagaagcgag cccaccgcag agccacaaaa
     9541 tgccaccgcc agccagttct gcgctgatat ccgggcgccg accgacgcat gaatggcaat
     9601 gccaaggaga ccaccagccc ccatcattga ggagaacagc ccgagctctg ctacttggcg
     9661 tcctgcatct acaaacagcg caggcatgat gacgctgccg ttggcgccaa cgatgcccac
     9721 gaagatcatc actataccaa agagagggcg cagcaggggt tcgctccaga gaaaagcgac
     9781 gccggcgcgc atggagagag tcgccgtcgt ggtcatcgtc cgagcggcac gcgcgggaag
     9841 cacccacgcg ccgagcagac ctgcaaggac ggagcagaac gccgtcagcc cgagcgttgg
     9901 cgcagcgcca agcaggccga ttgcggcccc cccaagggcc gggccaccta gaatcgcgac
     9961 gttcccgatc accgctttca gtgacgagac gcgctcaacg gagagcccgg cgacgtggcc
    10021 gagtttgggc agctcactgt cctgcgcggc cataccgggt gcgtcgaacg cggcaccgag
    10081 caccacgcaa gcgatcagcc cagtgttcga gagggcgcca acggcatcga gcagtgggat
    10141 gctcgccatg gccacgccgc ccaccacacc cgagatcaat gcgacgggcg cgcgcccgaa
    10201 ccgatcgacg aggccaccac caacccacgc gccgatgatg gtcgcgatga cgctgctagc
    10261 ggccgtggcg cccgcccagg ccgcgctctt tgtatgagac aggacgaacc atggaagcgc
    10321 gagggccgcc accgcgttgc cgatccggaa gagaaaggtc gccgcgaaca gcgtcgcgag
    10381 cgggctatat cgacgttcgc tcattccgct gcggcgagct gcgccttcgc cgcagcgagg
    10441 tactcttcgt tacccgagtc gagggcgaag agtgcgtagg tgaccgcccc gaacgcaagg
    10501 cgctccgcga tgtggtgggc gagccgcggc cacacccggc caccggccgc ttcatacgtg
    10561 aggaggagct tcgcgagccc ctcttcacca aagaccataa ggtgcgcggc catgtcgatg
    10621 gcagggtcat caacgcgggc ctcgctccag tcgatcatcc cgctgacgcg ctccgtgttg
    10681 tcgatgagca catggcccac gtagagatcg ccatgcacca ccacggagaa atctggccac
    10741 gacgaatcgt cgtcgagcca gcgctgccac cggtggaggc gcttgtcgtt caccacgaac
    10801 tcgcgtcgga cgcggtcaac gtcgtcggcc accttctgac gggcctgcgt cggtgtacgg
    10861 atgagcatcc ccgcatccac ggcggcggaa atggggacgg catgcagggc ggcgagcgcg
    10921 gtcgcgaagc tctccgcgaa gacctccgag tcctgcggca cgacccagtc gggcgtggac
    10981 gaaccaggct ggatgaccat cgcagtcgag tcttcgagca tgggataggc aacgagctcg
    11041 gcgttggcca cgcgccagtc cggcaccgcg aacggcaggc gattcttgag cattgccagc
    11101 acccgcgcct ctggttcgac cttcgcgctt acctcggctc ggcgcgggat gcgcagcacc
    11161 caccgacgtc catcgtcgac ggtggcgatc acgatcctat agtcgagccc aagctcattg
    11221 acagtcagcg ggccatggag cttgagccca tgtcgggctg caagtgcgta cagttgggag
    11281 gtatcggcgg tcgtgactac ggtcatgatt cactcctgag ggcttgacgg gtttagccac
    11341 ctaaatgtaa cagtcacgtc ggttatattc aatccggcac tgttgcaaag ttagcgatga
    11401 ggcagccttt tgtcttattc aaaggcctta catttcaaaa actctgctta ccaggcgcat
    11461 ttcgcccagg ggatcaccat aataaaatgc tgaggcctgg cctttgcgta gtgcacgcat
    11521 cacctcaata cctttgatgg tggcgtaagc cgtcttcatg gatttaaatc ccagcgtggc
    11581 gccgattatc cgtttcagtt tgccatgatc gcattcaatc acgttgttcc ggtacttaat
    11641 ctgtcggtgt tcaacgtcag acgggcaccg gccttcgcgt ttgagcagag caagcgcgcg
    11701 accataggcg ggcgctttat ccgtgttgat gaatcgcggg atctgccact tcttcacgtt
    11761 gttgaggatt ttacccagaa accggtatgc agctttgctg ttacgacggg aggaga
//
LOCUS       DAETQN010000128        11809 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.128, whole genome shotgun sequence.
ACCESSION   DAETQN010000128 DAETQN010000000
VERSION     DAETQN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11809
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.128"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..487
                     /locus_tag="K0W68_003527"
     CDS             <1..487
                     /locus_tag="K0W68_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3074988.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            539..1474
                     /gene="ttcA"
                     /locus_tag="K0W68_003528"
     CDS             539..1474
                     /gene="ttcA"
                     /locus_tag="K0W68_003528"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBI3074989.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="K0W68_003529"
     CDS             complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="K0W68_003529"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBI3074990.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="K0W68_003530"
     CDS             complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="K0W68_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBI3074991.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4692..5924
                     /gene="dgcM"
                     /locus_tag="K0W68_003531"
     CDS             4692..5924
                     /gene="dgcM"
                     /locus_tag="K0W68_003531"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HBI3074992.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="K0W68_003532"
     CDS             complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="K0W68_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBI3074993.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6838..7746)
                     /locus_tag="K0W68_003533"
     CDS             complement(6838..7746)
                     /locus_tag="K0W68_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3074994.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7922..9232
                     /gene="abgA"
                     /locus_tag="K0W68_003534"
     CDS             7922..9232
                     /gene="abgA"
                     /locus_tag="K0W68_003534"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HBI3074995.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9232..10677
                     /gene="abgB"
                     /locus_tag="K0W68_003535"
     CDS             9232..10677
                     /gene="abgB"
                     /locus_tag="K0W68_003535"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HBI3074996.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10714..>11809
                     /locus_tag="K0W68_003536"
     CDS             10714..>11809
                     /locus_tag="K0W68_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBI3074997.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNM"
BASE COUNT         3005 a         2999 c         2929 g         2876 t
ORIGIN      
        1 gaatgtcaga agaaacctga cgatacttga tatgttcggt cccccaaaaa caaatgccgg
       61 gatccggacg gtaacattac tgcagccggc tcttgaagca ctgaaggagc aatacaaact
      121 gaccgggcat catcgcaaaa gcgaaatcac tttttatcat cgggagtacg gcagaactga
      181 aaagcaaaaa ctgcattttg ttttcatgcc cagggtgtgt aacgaaaaac agaaacctta
      241 ttactcggta agcagtttgg gtgcaagatg gaatgcagca gtaaaacgtg ctggtattcg
      301 ccgccgtaat ccgtaccata cgcgacatac ttttgcctgc tggctgttga cggcaggagc
      361 gaacccggca tttatagcca gccagatggg gcatgaaact gcgcaaatgg tgtatgaaat
      421 ttacggtatg tggattgatg acatgaacga cgaacagata gccatgttga atgcgcggtt
      481 atcgtagttg caaagtttgc ccccaatttg ccccatttag taccagagaa ctgaaataat
      541 gcaagaaaat caacaaatta caaagaaaga acaatacaac ctgaacaaat tacaaaaacg
      601 tctgcgtcgt aacgtgggcg aagccattgc tgacttcaat atgattgaag aaggcgatcg
      661 catcatggtt tgcctctccg ggggtaaaga cagctatacc atgctggaga ttctgcgcaa
      721 tttgcagcaa agcgcgccaa tcaatttttc gctggtggct gttaacctcg atcaaaagca
      781 accgggcttc ccggaacacg ttctgcccga gtatcttgaa aagctgggcg ttgagtacaa
      841 gattgtcgaa gagaacactt acggtatcgt gaaagagaag attccagagg gcaaaaccac
      901 ttgctcactg tgttctcgcc ttcgtcgcgg tatcctttat cgtaccgcaa cggaactggg
      961 ggcgacgaag atcgcgctgg gtcaccatcg tgacgatatc ctgcagacgc tgttcttaaa
     1021 tatgttctac ggcggcaaga tgaaaggtat gcctccgaaa ctgatgagcg atgatggcaa
     1081 acatatcgtt attcgtccgc tggcctactg ccgcgagaaa gatattcagc gatttgccga
     1141 tgcaaaagcg ttcccgatta ttccgtgcaa cctgtgcggt tcacagccta acctgcaacg
     1201 tcaggtgatt gctgacatgt tgcgtgactg ggataaacgt tatccagggc gtatcgagac
     1261 gatgttcagc gcgatgcaga atgtggtgcc gtcgcatctg tgcgatacca acctgttcga
     1321 tttcaaaggc atcacccacg gttctgaagt ggttaacggg ggtgatctgg cgtttgatcg
     1381 cgaagagatc ccactacaac cggcgggctg gcagccagaa gaagatgaaa atcagttgga
     1441 tgagttacgg ctgaatgtgg ttgaagtgaa ataaccagga tggcgcccga tgcgcaagcg
     1501 tatcgggcta ctcttatgga ggccggataa gacgcggcca gcgtcgcatc cggcaatccc
     1561 gaataagatg tttactcttg cacccggcaa ttcaacattt cattatttta ataaccgcac
     1621 ccggctcgtt tttcctttaa tcttcccgcc ctgtaactgt ttccatgctt tatgagcaac
     1681 agcctgacgg accgcgacat agacatgcgc cggatgcacg gcgattttgc caatatctgc
     1741 gccatcaagc ccgatatctc ctgtcagtgc acctaataca tcacccgggc gcattttggc
     1801 ttttttccca ccatcgatac acaacgttgc catttctgct tccagcggca caatggaact
     1861 attagctggc ggcgtttgcc agttaagttt tatctgcaac atgtcagaaa tgatattggc
     1921 ccgctgtgct tcttccggag cacagaaact gatcgccaga ccgctatttc ctgcacgagc
     1981 tgtacggccg atgcgatgta catgaacttc agggtcccac gccagctcaa agttcaccac
     2041 cagctcaagc gatttaatat ccagaccacg cgcagcaaca tcagtcgcaa ccagtacacg
     2101 ggcgctaccg ttagcaaaac gtaccagggt ctgatcgcga tcgcgttgct ccaaatcgcc
     2161 gtgtaatgac aatgcacttt gccctacttc attcagcgcg tcgcagacag cctggcaatc
     2221 ttttttggta ttgcaaaaca ccacgcaaga ggatggctga tgcaagctta ataaccgttg
     2281 caacagagga attttgcctt tgctggatgt ctcataaaat tgttgttcaa tgggtggcaa
     2341 agcatctgtt gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattac
     2401 ggcaatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa
     2461 acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc
     2521 catcaccaaa gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa
     2581 acgacccggc gttgccacga taatatgcgg cgcatgttgc aacgaatcac gctgcatacc
     2641 gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg
     2701 acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg
     2761 ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt
     2821 gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc
     2881 ggcggcctgc accggcgtca tagttaaata acccaactca ttaaggttcg tgagttgggc
     2941 gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg
     3001 caaagttagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt
     3061 tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga
     3121 acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata
     3181 tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa
     3241 aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa
     3301 aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa
     3361 gggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt
     3421 tttgtataca gacagccgga aaaattgctt ttgttacaac catttactac ggtgcaacca
     3481 taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg
     3541 ccacccgccg ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact
     3601 gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg
     3661 catcacctga gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc
     3721 gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct
     3781 catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata
     3841 gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg
     3901 ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc
     3961 gctggaatga tcggtcaacg catcgcacac atccaccagc caccctccgc aatcggtcgg
     4021 acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg
     4081 ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg
     4141 ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgagacggct
     4201 gactcggggc cgcgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg
     4261 tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg
     4321 atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag
     4381 catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccacagt
     4441 gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct
     4501 catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca
     4561 aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat
     4621 tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata
     4681 acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct
     4741 ggatcgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga
     4801 tgcaagacct cacgtttagt cagctacgaa ccggtcccta ttccgtctcc tcacaaaaag
     4861 aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg
     4921 ttcagcgtaa agaagaggaa acagcattaa actgtcggct tgttttgcga aagctgacag
     4981 aagcagaacc ggtgattatt ttcgaaggta tcgaagcgtc ggcaacgctg ggtttaaaag
     5041 ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga
     5101 ctaactctgc accaatgttg ttgattgacc cctcacgaga tggccaaatc gtcgatgcta
     5161 acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct
     5221 gggaaataaa tatgctcggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac
     5281 ccggtggtca taaacctctg aattttattc ataagctggc ggatggttcg actcgtcatg
     5341 tgcagaccta cgccgggccg attgaaattt atggcgacaa gctcatgtta tgtattgtac
     5401 atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgcc caccatgacg
     5461 cgatgaccgg attactgaat cggcgacagt tttatcacat taccgagcca ggccaaatgc
     5521 agcatctcgc catcgcccag gattacagct tgttgctcat cgacaccgat cgttttaaac
     5581 acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgca
     5641 ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg
     5701 tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg
     5761 taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg
     5821 cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt
     5881 atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc
     5941 gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt
     6001 tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgca gtgcaatatg
     6061 cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat
     6121 gcgatttatc atcccgtcct ttgccatgaa taatcagcac gttacgcaaa ccatcagcca
     6181 tggcttgttg aatgaaactg aacatcattt tgcggcactc ttccaccggc tggcgcaaaa
     6241 gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgtt
     6301 gcaacccttc ccgccgaaac tccagcggct gacttagtgg gatgatgtcg agaaatccgg
     6361 tggtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg
     6421 ttggatgcca gtggacatcg gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa
     6481 gaaacagcga tttgtcgtca aggttcatgt tacatcctcc cgcaattaag agcgcgatat
     6541 gataaccaga ccgggtcggt ccaacaacgt attacccaaa attccagtaa taagttccaa
     6601 atattgccga tattttaagc aaaatgctta tgcatgatta ttcattcacg atattaataa
     6661 tgtaacttat attttcgtga aatctgtcac tgaagaaaat tggcaactaa aggttaaaac
     6721 cgttataaca cagtcaccgg cgcagaggag acaatgccgg atttaagacg cggatgcact
     6781 gctgtgtgta ctgtagagtc tggcggatgt cgacagactc tattttttta tgcagtttta
     6841 actttgcaga tagccgcatt ctcgccggaa ttgcgtgatt aatgacgcgg tcagtggtgt
     6901 ctggcgacta tcacgccgct gaatcaaata ataggccgct ttcggtaaaa tttcgctaac
     6961 cggcaacatc accagcccct gtccgtgcaa gggatcgcag cccatttctt caggcagtat
     7021 gctgagaaaa tcgctttttg ccaccagact gatacaggct gagaacgtct cgcagactac
     7081 accgacctgt ggcgtttgcg cctgatcgtc aagcaattca ctcaactgtt tgtagtaact
     7141 gccgtgtggc gtcggcattg tccagctgta atccagtaac tgtttgatcg aacgggcacc
     7201 aatggcgggg tgtcccgggc ggcaaaagat cgcgaattgc ttttccagta atttctcaaa
     7261 agtaaattcg tggtcgtacg gtccctgata ataggtattg atggtgaaat ccaattctcc
     7321 ctgacgcaat tcattaatca tcgacaccag ttgcccttcc ataatgcgta cttttacctg
     7381 cggatgctgc tgatgaaaac gagatatgac agctggcatc agactgcggg aaatactggc
     7441 ccccatgccg atattaatct gccctgccag ttgcccttgt cgttggcgaa tatcctcttg
     7501 ggctgcgcgc agctcttcaa gaataagact ggcgtgctga taaaaacttt caccggcatc
     7561 agttaacgtc acgcctttac tacggcgaaa aaagagttgc gccgctaatc cttcttccag
     7621 ctcctgaata gatttactca gtgccggttg cgacatattc aacattcggc tcgctccgcg
     7681 aatgctgccc tgacgagcca cttcaacaaa agcccgaatt tgatgaattt ttacctgaaa
     7741 agccatgacg ccaccgataa ccgttattta tcagaccaaa gaaactggca tctactttaa
     7801 tgcagatgat tgtgtcaggg taatttatga acggttaaaa ctgtgaaaaa tcagttagtg
     7861 ataagtaaaa actatcgcta cgtgaaccgg gtcacacttt ttactgatga cgggaaaggt
     7921 tatggagtct ttgaatcaat ttgttaattc gcttgcccca aaattatcgc actggcgacg
     7981 tgattttcat cactatgcag agtctggctg ggtggaattc cgcactgcca cccttgttgc
     8041 ggaagaattg cagcagctcg gctattcact ggcgctgggc cgcgaagtag ttaatgaaag
     8101 tagccggatg ggactacctg atgaactcac tctacaacgc gaattcgagc gcgctcgtca
     8161 acagggtgcg ctagcacaat ggattgcggt ttttgaaggt ggtttcactg gtatcgtcgc
     8221 caccctggat accggtcgcc ccggtccggt gatggctttc cgtgtcgata tggacgcgct
     8281 ggatctcagt gaagagcagg atgtcagcca tcgcccctac cgcgacggtt ttgcgtcatg
     8341 taacgccgga atgatgcatg cctgtggtca tgatggacat accgccattg ggcttgggct
     8401 ggcgcatacc cttaaacaat tcgagtccgg actacatggc gtcatcaaac tgatttttca
     8461 gcctgcagag gaaggtacgc gtggcgcgcg ggcgatggtc gatgcaggtg tcgtagatga
     8521 tgttgattat tttactgccg tgcacattgg cactggcgta cctgcgggca ccgtggtgtg
     8581 cggcagtgat aattttatgg caaccaccaa atttgacgcg cacttcaccg gtaccgccgc
     8641 tcacgcaggc gcaaaaccag aagacggtca caatgccttg ttggcggcag cacaagccac
     8701 tcttgcactg catgcaatcg ccccgcacag cgaaggagct tccagagtaa acgtgggcgt
     8761 tatgcaggca ggaagcggtc gtaacgttgt tcctgcctcg gcgttgctga aagtggaaac
     8821 acgcggggcc agcgacgtca ttaatcaata tgtttttgac cgtgcacaac aagcgattca
     8881 gggcgcagca accatgtatg gtgtcggcgt tgaaactcgt ctgatgggtg cagttaccgc
     8941 cagttctcct tcgccgcaat gggtcgcatg gttgcaaagt caggcggctc aggtcgcggg
     9001 ggtcaatcag gccattgaac gtgttgaagc gcctgcgggt tccgaagatg ccacattaat
     9061 gatggcccgc gtgcagcaac atcaagggca agcctcctac gtggtgtttg gcacacagct
     9121 ggcggcaggt catcacaacg aaaaattcga ttttgacgag caggttctcg ctattgccgt
     9181 cgaaacgctg gcgcgcaccg cgctcaattt tccctggacg cgaggtatct gatgcaggaa
     9241 atctatcgtt ttatcgacga tgcgattgaa gccgatcgcc aacgttatac cgatattgcc
     9301 gatcaaatct gggatcatcc agaaacacgt tttgaagagt tctggtcagc ggagcatctg
     9361 gcttcggctc tggaatctgc aggcttcacc gttacccgca acgtaggcaa tatcccaaat
     9421 gcctttattg cttcgtttgg tcaaggcaaa ccggttatcg ccctgctggg agaatatgac
     9481 gccctggcag gtttaagtca gcaagctggt tgcgcgcaac ctacatccgc gacgcccggt
     9541 gaaaatggtc acggttgcgg acacaatttg cggggaaccg ccgcctttgc cgctgcaata
     9601 gccgtcaaga aatggctgga acaatatggg caaggcggca cagtgcgctt ttatggttgt
     9661 cctggcgaag aaggcggctc gggtaaaacg ttcatggtcc gcgagggggt atttgatgat
     9721 gtggatgcgg cactcacctg gcacccggaa gcctttgccg gtatgttcaa tacccgcacg
     9781 ctggcaaaca ttcaggcatc atggcgcttt aaagggatcg cagcacatgc cgcgaattcc
     9841 cctcatttgg gacgcagcgc ccttgatgcc gtaacgttga tgaccactgg caccaacttc
     9901 ctcaacgaac atattattga aaaagcgcgc gtacactatg ccatcacaaa tagcggcggg
     9961 atctcgccca acgtggtcca ggcgcaggca gaagtgcttt atcttatccg cgcccccgaa
    10021 atgaccgacg tgcagcatat ttatgatcgg gtcgccaaaa tcgccgaagg tgcggcattg
    10081 atgaccgaaa ccacggttga atgccgcttc gacaaagcct gttccagtta tctcccgaat
    10141 cgcaccttag aaaatgccat gtaccatgcc ctatcccatt ttggtacccc ggaatggaac
    10201 tccgaagaac tggcttttgc gaaacaaatt caggctacgc tcacccccaa cgatcggcaa
    10261 aacagtctga ataatatcgc cgcaaccggt ggcgaaaacg gcaaggcttt tgcactacgt
    10321 catcgtgaaa cggtactggc gaatgaggtc gctccatatg ccgccaccga taacgtgctt
    10381 gcggcatcga ctgatgtcgg cgacgttagt tggaaactgc ctgttgccca gtgtttcagc
    10441 ccctgttttg ccgtcggtac accgctacat acgtggcaac tggttagcca ggggcgaaca
    10501 tctattgctc ataaaggaat gctgctggcg gcgaaaacta tggcagcaac cacagtcaat
    10561 ctcttcattg attcagggct attgcaagaa tgccaacaag agcatcagca agtaacggac
    10621 acgcaaccgt atcactgccc tatcccgaaa aacgtgacac cgtcaccttt aaaataacaa
    10681 caacaacgca aacacaacaa ccgaggaatg cccatgagta tgtcatccat accgtcgtcc
    10741 tcccaatccg ggaagctcta tggctgggtc gaaagaattg gtaacaaggt tctccatcct
    10801 tttttgctct ttatctattt gattatcgta ctcatggtga cgacggcaat tttgtcggcc
    10861 tttggcgtca gtgcgaaaaa cccgaccgat ggaacgccgg tggtggtgaa aaacctgctc
    10921 agtgtggaag gattacactg gtctttaccc aatgttatta aaaactttag cggttttgct
    10981 ccacttggtg cgatcctggc gctggtttta ggtgccggtc tggcggagcg cgtcggctta
    11041 ctgccagcgc taatggttaa aatggcatcg catgttaatg cccgctacgc cagttatatg
    11101 gtgctgttta ttgctttttt cagccacatt tcttccgatg cgacgttagt gatcatgcta
    11161 ccgatgggtg cgctgatttt tctggcggtg ggcaggcatc cagttgcagg tttactggct
    11221 gccattgcag gcgtaggttg cggctttacg gctaatttac tgattgtcac aaccgacgtg
    11281 ttgctgtcgg ggatcagcac ggaagcggcg gctgcgttca atccgcaaat gcacgtcagt
    11341 gtaattgata actggtattt tatggccagc tccgtagtcg tactgacgat tgttggcggc
    11401 ctgataaccg acaaaatcat cgagccacgg ttaggtcaat ggcagggaaa cagcgatgag
    11461 aaactgcaga cattgaccga aagtcagcgt tttggtttac gcatagcagg tgtcgtatcg
    11521 ctacttttta ttgctgcgat tgcgctgatg gtgatcccgg aaaacgggat attgcgcgat
    11581 ccgattaatc acaccgtgat gccatcaccc tttattaaag gtatcgtgcc actgatcatt
    11641 ctttttttct ttgttgtctc gctggcttat ggcatcgcta cccgcacaat tcgacgtcag
    11701 gcggatttac cgcagttaat gattgaaccg atgaaagaga tggcaggatt tatcgtgatg
    11761 gtttttcccc tcgcccagtt tgtcgccatg tttaactgga gcaacatgg
//
LOCUS       DAETQN010000129        11779 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.129, whole genome shotgun sequence.
ACCESSION   DAETQN010000129 DAETQN010000000
VERSION     DAETQN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.129"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..611)
                     /locus_tag="K0W68_003537"
     CDS             complement(<1..611)
                     /locus_tag="K0W68_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBI3074998.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /gene="fumB"
                     /locus_tag="K0W68_003538"
     CDS             complement(734..2380)
                     /gene="fumB"
                     /locus_tag="K0W68_003538"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBI3074999.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="K0W68_003539"
     CDS             complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="K0W68_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBI3075000.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="K0W68_003540"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="K0W68_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBI3075001.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /gene="dcuS"
                     /locus_tag="K0W68_003541"
     CDS             complement(5085..6716)
                     /gene="dcuS"
                     /locus_tag="K0W68_003541"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBI3075002.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /locus_tag="K0W68_003542"
     CDS             6897..7127
                     /locus_tag="K0W68_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBI3075003.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="K0W68_003543"
     CDS             7139..7411
                     /locus_tag="K0W68_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI3075004.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..7934
                     /gene="ghoS"
                     /locus_tag="K0W68_003544"
     CDS             7638..7934
                     /gene="ghoS"
                     /locus_tag="K0W68_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBI3075005.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7962..8135
                     /gene="ghoT"
                     /locus_tag="K0W68_003545"
     CDS             7962..8135
                     /gene="ghoT"
                     /locus_tag="K0W68_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HBI3075006.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="K0W68_003546"
     CDS             complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="K0W68_003546"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBI3075007.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="K0W68_003547"
     CDS             complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="K0W68_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HBI3075008.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11524..>11779)
                     /locus_tag="K0W68_003548"
     CDS             complement(11524..>11779)
                     /locus_tag="K0W68_003548"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBI3075009.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGTHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3044 a         2856 c         2892 g         2987 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcagga gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatgggttt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggtgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
     7741 atttctcttc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaattcct
     7801 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
     7861 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
     7921 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
     7981 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
     8041 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
     8101 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
     8161 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
     8221 gtcgcctgac tgcttcgttc attaaagtga aatttatttc tgtgggcgca tcgccgggaa
     8281 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
     8341 aatacccaga ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc
     8401 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
     8461 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
     8521 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
     8581 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
     8641 atcaaagatc tccgtgataa tacgtcccag cccccagctt ttctctaccg taattccgat
     8701 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
     8761 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
     8821 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
     8881 cagtgactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
     8941 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
     9001 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
     9061 acgcagatac atatctaaat ccagagcatt atgatgggta ataaacgggc gagcagatgc
     9121 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
     9181 gaactgacgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
     9241 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
     9301 gaatttatct ggtaaaggac gtagtgcttt agtcagcagg cgcaactcag tacagtgaat
     9361 ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
     9421 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
     9481 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
     9541 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
     9601 attcttcgca tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt
     9661 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
     9721 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
     9781 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
     9841 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
     9901 tcactaataa ataaccatca aaatggttta aatgacttat taatctcgat ttactagtgt
     9961 gattaactga aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc
    10021 tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caatacgacg
    10081 atgatcgcga cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagct
    10141 gcaattacca taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc
    10201 cagttggcga ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc
    10261 agacgcgtga tttgcgcaat cgccaccggg tcaataaaga gttctgcaaa gcccattagc
    10321 gccaacccgg atatcatcac gcccattgac gcttgaccgt cagccgctgc atgtcgggca
    10381 tcaaatgcca acaacataaa gccacaagcc atcagcagta agccaaaggc aaacttcagc
    10441 cagacgcgca atgttgagtt gccgcggctt tctggcgacg ccagccaggc cagtacaacc
    10501 ccagcgagca tcactgcaat tgcattcacc gactggaata gtgctgtagg tacttcaata
    10561 ttgaatgcct gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt
    10621 tgtgccagta cccagaacaa cgtcccgaca aacatcaaca atacaatttg ccaaagagca
    10681 cggcggtgtt cggggaattt gatcatcatg cgggcaatga tttgtgcggc aatgaggcaa
    10741 acgatcgcca gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg
    10801 gctaaacaga gcatcaccac taaccagctc catactggta aggcaaattt gacgctagtg
    10861 agcgcttttt tatccatact acgcgtggac tggaaatgac gatgaccgct taagaaaatc
    10921 aacaaaccga taaacatgcc gccacccgca agggcaaagc caacatgcca tccataccac
    10981 tgagcagcca ggccgcaggc gattggggcg gcgatagaac cgatattgcc cgcagcatac
    11041 agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt cgtagagctc gccaagcaaa
    11101 cagctgatgt ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac
    11161 aggctaaagg ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc
    11221 gcgccggcaa tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga
    11281 ataggggtaa cgtaaaccag agaagcatat gcgctgaaca ggctgatggc atggttatca
    11341 tcaaaaccaa gctgatgggt gagatagaga atgagtaagg cacgcatgcc gtaaaaactg
    11401 aagtactccc agatttggat cgccacgata tagtatatcg cgcgcggctg tgagggtgtt
    11461 ttcatgtgtt ctccttatga gcaaaaaagg gaagtggcaa gccacttccc ttgtacgagc
    11521 taattatttt ttgctttctt ctttcaatac cttaacggta tagcggccat cagcctgacg
    11581 gtatgcaccg tgaatatcgg tttcaaatcc cggatagtga gtgccgattt cacacagcat
    11641 ctgcaggaac tccagaaccg gacggctttc ttcggtgatc atttcacccg gcattaccag
    11701 aggaactccc ggcggatacg gaaggatcat attggcgtta atacgaccta ccatttcgtc
    11761 gaggtaaact tcttcggtc
//
LOCUS       DAETQN010000130        11428 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.130, whole genome shotgun sequence.
ACCESSION   DAETQN010000130 DAETQN010000000
VERSION     DAETQN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.130"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="K0W68_003549"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="K0W68_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBI3075010.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="K0W68_003550"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="K0W68_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBI3075011.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="K0W68_003551"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="K0W68_003551"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBI3075012.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="K0W68_003552"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="K0W68_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBI3075013.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="K0W68_003553"
     CDS             complement(3928..5214)
                     /locus_tag="K0W68_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBI3075014.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="K0W68_003554"
     CDS             complement(5235..6026)
                     /locus_tag="K0W68_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBI3075015.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="K0W68_003555"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="K0W68_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBI3075016.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="K0W68_003556"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="K0W68_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBI3075017.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="K0W68_003557"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="K0W68_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBI3075018.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="K0W68_003558"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="K0W68_003558"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBI3075019.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="K0W68_003559"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="K0W68_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBI3075020.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="K0W68_003560"
     CDS             complement(9882..10364)
                     /locus_tag="K0W68_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBI3075021.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="K0W68_003561"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="K0W68_003561"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HBI3075022.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2803 a         2747 c         3061 g         2817 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccag ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat catcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggtggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctact aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAETQN010000131        11355 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.131, whole genome shotgun sequence.
ACCESSION   DAETQN010000131 DAETQN010000000
VERSION     DAETQN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.131"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..535)
                     /gene="espL1"
                     /locus_tag="K0W68_003562"
     CDS             complement(<1..535)
                     /gene="espL1"
                     /locus_tag="K0W68_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HBI3075023.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQY"
     gene            1060..2988
                     /gene="thrS"
                     /locus_tag="K0W68_003563"
     CDS             1060..2988
                     /gene="thrS"
                     /locus_tag="K0W68_003563"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBI3075024.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2992..3534
                     /gene="infC"
                     /locus_tag="K0W68_003564"
     CDS             2992..3534
                     /gene="infC"
                     /locus_tag="K0W68_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBI3075025.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3631..3828
                     /gene="rpmI"
                     /locus_tag="K0W68_003565"
     CDS             3631..3828
                     /gene="rpmI"
                     /locus_tag="K0W68_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBI3075026.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3881..4237
                     /gene="rplT"
                     /locus_tag="K0W68_003566"
     CDS             3881..4237
                     /gene="rplT"
                     /locus_tag="K0W68_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBI3075027.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4354..4478
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4360..4404
                     /gene="pheM"
                     /locus_tag="K0W68_003567"
     CDS             4360..4404
                     /gene="pheM"
                     /locus_tag="K0W68_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBI3075028.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4687..5670
                     /gene="pheS"
                     /locus_tag="K0W68_003568"
     CDS             4687..5670
                     /gene="pheS"
                     /locus_tag="K0W68_003568"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBI3075029.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5685..8072
                     /gene="pheT"
                     /locus_tag="K0W68_003569"
     CDS             5685..8072
                     /gene="pheT"
                     /locus_tag="K0W68_003569"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBI3075030.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8077..8376
                     /gene="ihfA"
                     /locus_tag="K0W68_003570"
     CDS             8077..8376
                     /gene="ihfA"
                     /locus_tag="K0W68_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBI3075031.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8477..9457
                     /gene="btuC"
                     /locus_tag="K0W68_003571"
     CDS             8477..9457
                     /gene="btuC"
                     /locus_tag="K0W68_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBI3075032.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9520..10071
                     /gene="btuE"
                     /locus_tag="K0W68_003572"
     CDS             9520..10071
                     /gene="btuE"
                     /locus_tag="K0W68_003572"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBI3075033.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10071..10820
                     /gene="btuD"
                     /locus_tag="K0W68_003573"
     CDS             10071..10820
                     /gene="btuD"
                     /locus_tag="K0W68_003573"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBI3075034.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10898..>11355
                     /locus_tag="K0W68_003574"
     CDS             10898..>11355
                     /locus_tag="K0W68_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBI3075035.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2873 a         2631 c         3055 g         2796 t
ORIGIN      
        1 cgtattgcaa tgacagatgg cgacaaaaaa tagcgtcaga aggagattgc gaaaaaaatg
       61 cactaccatt gagatttact gttggttgta tagttttttc tctatggatt tttttagaca
      121 gtttcataca atggttacga tcttcggcag ttaactgttc ctgttcaata taatccagta
      181 tgcttcgcaa atggttagcc ttaataggcg tggtttccag aaataaggga atgatttctt
      241 ttttatagtg taatgtaatg agtatatttt ctgcatggat gggtgaagtg gcaaagttaa
      301 tgataaaaag ggcagtggaa taacaatttt gtgaaatttt tcgacagttt gcggtaaatt
      361 cctgagtaac acagtatgac agcatcgaac ttaagtcaga ggtatcaagt agggtgatga
      421 gttggcgcgc aaattgatta ataggctctg atgaaggcgt tggtatgtcg ctatcgatgt
      481 gcaatctatg aggagactta taatgggttc tgataatgat atcgttttga ctcataatct
      541 attcgcctta tatatgtctg gaggttttga ggatgatgat taattattat cgccagaacg
      601 aaatgtgcac gaaaaattgc tataagaatt gtgtcacttt tatcattgtg atatgaccac
      661 ttcgtcatta atctcatgag cgtttaaaat actaccgcca aatcacgcaa cgaactttgc
      721 tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa cctttcagac gcaccgtgat
      781 gttatcagtg gttctttaag cgttttgctg gtgtactcac tacaaacgaa ttgcgaatcg
      841 aatcaatgtg aaacggaaag gtacaatctc ccttttttta gtgttaacat cgttcaaccg
      901 ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct gattgcgaac caatttagca
      961 tttgttggct agatggtttc gcaatgaact gttaataaac aaatttttct ttgtatgtga
     1021 tctttcgtgt gggtcaccac tgcaaattaa ggatataaaa tgcctgttat aactcttcct
     1081 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
     1141 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
     1201 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
     1261 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
     1321 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
     1381 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
     1441 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1501 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1561 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1621 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1681 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1741 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1801 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1861 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1921 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1981 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     2041 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     2101 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     2161 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     2221 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     2281 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     2341 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     2401 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     2461 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2521 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2581 gtaatgattc accgcgcaat tctggggtcg atggaacgtt tcatcggtat cctgaccgaa
     2641 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2701 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2761 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2821 cgtcgcgtcc catatatgtt ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2881 gttcgcaccc gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag
     2941 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     3001 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     3061 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     3121 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     3181 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     3241 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tggtacagat
     3301 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     3361 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     3421 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tggtcgaatc cttcccaacg
     3481 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3541 caagtagcac tgtctgtgga gccttcgggc ttcacaggct ttgttcgcct ttgtttcgtt
     3601 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3661 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3721 cacattctga ccaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3781 tccaaaggcg atctgggcct ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3841 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3901 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3961 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     4021 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     4081 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     4141 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     4201 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     4261 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     4321 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     4381 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     4441 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4501 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagcg cggagaaacc
     4561 ggagcgtatt taagtacgtg agaatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4621 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4681 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4741 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4801 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4861 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4921 gaaagcgcag cgctgaatgc gcgtctggcg gcggaaacga ttgatgtctc cctgctaggt
     4981 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     5041 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     5101 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     5161 tggtttgaca ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     5221 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gcgtttatcg taacgactac
     5281 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     5341 agctttacca acctgaaagg cactctgcac gatttcctgc gtaacttctt tgaggaagat
     5401 ttgcagattc gcttccgtcc ttcctacttc ccgttcaccg agccttctgc agaagttgac
     5461 gtcatgggta aaaacggtaa atggctggaa gtgctgggtt gcgggatggt gcatccgaac
     5521 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5581 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5641 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5701 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tgcgctggcg aatcaaatca
     5761 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5821 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5881 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg tggtgcgcca aactgccgtc
     5941 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     6001 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttc tctgaactgg
     6061 gaatttctga cgatcacagc ggcattatcg aactgcctgc agatgcgccg attggcactg
     6121 acatccgcga atacctgaaa ctcgatgaca acaccatcga aatcagcgta acgccaaacc
     6181 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     6241 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     6301 cagtcgaagc gcctgaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     6361 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     6421 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6481 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa
     6541 cgctggtact gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6601 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6661 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6721 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6781 cggcactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6841 gtgaggctgg tccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6901 tcactttacg tcgtagcaaa ctggatcgcc tgatcggcca tcatattgct gatgaacagg
     6961 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gagtggcagg
     7021 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtt gaagaagtcg
     7081 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     7141 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     7201 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     7261 caggcgttga agccttactg ctgccaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     7321 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     7381 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     7441 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7501 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgatcttgaa tccgttctcg
     7561 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7621 cggggcaatc cgcagcgatt tatctgaaag gggaacgtat tggttttgtt ggagttgttc
     7681 atcctgaact ggaacgtaaa ctggatctta acggtcgcac tctggtgttc gaactggagt
     7741 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7801 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7861 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7921 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7981 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     8041 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     8101 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     8161 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     8221 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     8281 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     8341 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     8401 ctgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     8461 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8521 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8581 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8641 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8701 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8761 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8821 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8881 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8941 ctgtagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     9001 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     9061 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     9121 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     9181 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     9241 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     9301 acttcccggc tgcgcgctgg caggggcgag cgcagtgctg ctggccgata ttgtagcgcg
     9361 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     9421 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9481 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9541 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9601 ctgttgattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9661 aatattcaga aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9721 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9781 ggggtgacgt tcccgatgtt cagtaagatt gaagttaacg gcgaaggacg ccatccgctg
     9841 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9901 gcccgtatgg tcagcaaagg tcgtgcaccg ctgtaccctg atgatatttt atggaatttt
     9961 gagaaatttt tagttggcag ggacggattg gtcatccagc gtttttcccc ggatatgacg
    10021 ccggaaaacc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
    10081 tgatgcagtt acaggatgtt gcggaatcta cccgcctagg gccactttct ggcgaggttc
    10141 gggctgggga gatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
    10201 cacgaatggc cggaatgacc agcggtaagg gaagtattca gttcgcgggg caaccactgg
    10261 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
    10321 cgccgtttgc aatgccggtc tggcactatc tgacactgca tcagcacgat aaaacgcgta
    10381 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggacgtagca
    10441 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgctgcaaa
    10501 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10561 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgctctgtgt cagcaaggac
    10621 tggcgattgt gatgagcagt cacgatctca accacacatt gcgtcatgcg catcgggcgt
    10681 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10741 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10801 tgctgatttc gaccatctga tttcattagt atcttgaaaa tcgggtaatt acgacgctaa
    10861 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10921 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10981 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    11041 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    11101 aacgatgcgc gatcgtttcg atttgcagct gccccgagaa accaaagaac aagcctctat
    11161 cggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    11221 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    11281 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    11341 ctggcaggcg agacg
//
LOCUS       DAETQN010000132        11304 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.132, whole genome shotgun sequence.
ACCESSION   DAETQN010000132 DAETQN010000000
VERSION     DAETQN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.132"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..208
                     /locus_tag="K0W68_003575"
     CDS             <1..208
                     /locus_tag="K0W68_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3075036.1"
                     /translation="IVPLTVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFA
                     QTTKGVLETGIYRGVVSIYLSQDI"
     gene            300..2984
                     /locus_tag="K0W68_003576"
     CDS             300..2984
                     /locus_tag="K0W68_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HBI3075037.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2981..4069
                     /locus_tag="K0W68_003577"
     CDS             2981..4069
                     /locus_tag="K0W68_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HBI3075038.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="K0W68_003578"
                     /pseudo
     CDS             complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="K0W68_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5931..7343
                     /gene="uxaC"
                     /locus_tag="K0W68_003579"
                     /pseudo
     CDS             5931..7343
                     /gene="uxaC"
                     /locus_tag="K0W68_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
     gene            7358..8845
                     /gene="uxaA"
                     /locus_tag="K0W68_003580"
     CDS             7358..8845
                     /gene="uxaA"
                     /locus_tag="K0W68_003580"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBI3075039.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8928..9479
                     /locus_tag="K0W68_003581"
     CDS             8928..9479
                     /locus_tag="K0W68_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBI3075040.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9484..10728)
                     /gene="sstT"
                     /locus_tag="K0W68_003582"
     CDS             complement(9484..10728)
                     /gene="sstT"
                     /locus_tag="K0W68_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBI3075041.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2966 a         2772 c         2934 g         2632 t
ORIGIN      
        1 gattgttccg ctgacagtaa cctggggcgg tgaagaaatt aaagctgatg cggcaaccac
       61 ttttaccgca accaaaatct ttgcttccga tgcgttaact aacggttctc tggctaaaaa
      121 cctgatgttt gctcaaacca ccaaaggtgt tctggagaca ggcatctacc gtggtgtagt
      181 aagcatttat ctgtctcagg atatctaatc cagataacag aattaacacg taacaaaggg
      241 aggggtagcc ctcccttatt ttaactgttt tagtatttgt tatggatttc aggattgcca
      301 tggataaaaa attactggct cttttgatcc tggcgagtct cagcccggca gaggcgacat
      361 taaccgaaat tcccgcaggg tttgaggtta ttgctcaggg acagcaggag tatatcgagg
      421 tttatttttc agggaaaagt ctcggtaaat attatgcaat ggttaatctt gataccgtaa
      481 catttcttga tccagcaagt ctatataaca agctggaact ggatgtagac gatcagaaaa
      541 tcgcgcatat agtgaaagaa aaattatcgc agccgctagc tcaccacggt gaattggctt
      601 gcggttatgt acgtactgac tcagggtgtg gttttctgaa taccgatacg ctggaaataa
      661 tctataatga tgaagaaagt tcggcaacgt tgtttattaa tccgcaatgg aattcagctt
      721 tcgatgcgaa gtcattatat ttaaatccag acaaaaatac tgttaatgct tttatacatc
      781 agcaagacat caatgttctg gcacaggatg attaccaatc gttgtctatt cagggaaacg
      841 gtgcgctggg aataacagaa aatagttata ttggtgcaca ctggaatttc aacggttatg
      901 atgcagatga tgtcagtgac agtaatgctg acgtcagcga tctctattat cgttatgatt
      961 ttttacgtcg ttattatgtg caggcgggtc gcatggacaa ccgcacacta tttaatgcac
     1021 aaggcgggaa ctttaccttt aactttctgc cactcggtgc aattgatggg atgcgtatcg
     1081 ggtcgacact gagctattta aaccaggcgc aaagccagca aggaacccca gtaatggttc
     1141 tgctttcgcg caattctcgt gttgacgctt atcgtaatga gcaacttttg ggatcgtttt
     1201 atctcaatag tggttcgcaa tttattgata ccagttcctt tccgccaggc agctacagcg
     1261 tggcattaaa agtctacgaa aataaccaac tcacccgcac cgagcttgtg ccgtttacca
     1321 aaaccggcgg tctgactgac ggaaatgcgc aatggttctt acaggcaggt aaaactacat
     1381 cacaggtttc tgatgatgaa agttcagctt atcagctagg agtacgcctg ccattacatc
     1441 cgcaatatga gctctacgca gggctggcga atgccgatga tgtgagtgct ttcgagttag
     1501 gtaataactg gacggcagat ttaggcgggg tggggaatct tgcaatcagc gccagcgtgt
     1561 tccgtaacga tgacggcggc aaaggtgata tgcaacaggc caactggagt aatccgggat
     1621 ggccgacgtt gggcttttat cggaccaact ctgacggcga tgcttgtaca accgacagca
     1681 gagagagcta taacgcctta agctgttatg aaagtatttc cgcgacggtt tcacagaatt
     1741 ttgtcggctg gaatatgatg ctgggttata cccgcacaca aaataacact gatgatagtt
     1801 tgcgttggga taaacagcag agctttgaaa ataactatct tcgccagaca acagcgcaaa
     1861 gtatctctga aactgtacaa cttagcgctt cccgcgcttt tgtgatgcgt gactggattt
     1921 tgagtacgtc agtcggtgtt ttccatcgta atgacaacgg tggcgataac gacgacaacg
     1981 gcttgtactt atcgttttcg ttatctgaca cgccaacgat ggatagcaat aacaacagcc
     2041 attcaacaaa tgtgtctacg gattatcgtt atagcgaaca ggatggcgat caaacgtcat
     2101 ggcagttatc gcataccttt tataacgatt cattcagcca taaagaactt ggcgtaaccg
     2161 tcggaggcct gaacaccgat accataaaca gcgcggttaa cgggcgttgg gatgggcaat
     2221 acggaaatgt ctacgctacc gtatctgata gttatgaccg taagaatcat gatcatcttt
     2281 cggcctttac gggcacttat agctctacgc tggctgtcag ttgctatggc gttaatttgg
     2341 gagccagtgg tacagacgat ttgctgggtg cggtactggt ggatgtgaaa ggcttctctg
     2401 aacaggatga agagagtcag gatctgcaac tcgaagcgcg ggtggcaggc agccgaactt
     2461 tgcagcttgg tcaaagtgac agtgtgttgt tcccttatcc tggatttcag tctggttttg
     2521 ttgaggttaa cgacagtagc cagggcaatc agcaggggac aacaaacatc attaacggtg
     2581 cagggaatcg tgaattaatg ttgttgcctg gcaagctgcg ctatcgcgaa gtgtctgcca
     2641 gctttaacta caactatatc ggtcgtttat tactgccggc agcggtgaaa aaattcccga
     2701 ttgtggggct gaacagcgcc atgttactgg tagctgaaga tggaggattt acacttgaaa
     2761 ttaacggcag cgaaaaagag ctctatctgc tttccgggca gcaattcctt aagtgtccac
     2821 tgagtgttgt aaagaaacgc gccagcattc gttacagcgg agatgttact tgtagtgtgg
     2881 tgacttattc acaattaccg gagtcgattc aggttcaggc acagttgaaa cagcctaaat
     2941 tacgtggaaa cgttcagacg gcgcaaaggg aggttgcacc atgagaaatc gattgattgc
     3001 ggcgatattg ggcttgtttg gcacgctcac tggcgttcag gcagctcctg acgtgaccag
     3061 tgaaattacg tatgatttgg catctggtag agcggattat tacttctgga aggatgaagc
     3121 gtcggcagga aataatggat atatgtggta tgaatgttcg tatcctgacc tccaacaaac
     3181 ctgtacagct aatggaaata tatcgacagt acaaatctat ttaactgaac aacgcagtgg
     3241 gatgcgttgg ccggtaaaac tcaaaggatt taaaacagcc attgtaagta gcgatgaagc
     3301 gccgccagga tgcaaggggg gcaaagggct tcagacgaat cttaaggatt ctaatagatc
     3361 ttcatgtaca gaagatggtc aacattatta tatatacgat acaaagtttc ttacgctcta
     3421 ccttgagcag acagagatga agaatttgcc gattggtggc gtctggaagg ggaaagttaa
     3481 attacattcg aacagcccgg cccaggacta tttcgcaaat attaccctga atacgctcga
     3541 ccccaaccat attgacgtgt tcttcccgga gttcgcccac gccacgccaa gggtgcagtt
     3601 agacttgcat ccaacaggaa gcgttaacgg cagcaactac gcgcaagatc tgaccatgtt
     3661 ggacatgtgc ctgtacgatg gttttaacgg taatgccatc agttatgaaa tcatgctcaa
     3721 agatgaaggg cgaccagctg cagggcgcag agacggtgac ttctctatct atcgtcaagg
     3781 agggaccacc accgacgagg gagaacgcat tgattaccgg gtcaaaatgt acaacccgga
     3841 aaccggtggg caaattgatg tgcgcaataa tgaaaatatg gtctggaaca gcattaacct
     3901 gaaacgtgtg cgtccggtgg tactgcccgg tattcgctat gccgtgatgt gtgtgccgac
     3961 acccttgact ctggcagtcg ataaattcag cgtgatggat aaacaggccg ggtactacat
     4021 gggcaaattg tcagtaatct ttacgccttc cttgccaacc atcaattgac cccgcaggcc
     4081 agaaaaaact aaacaacgaa gccgcctgat aaggcggctt cgtcattaca acttacgtta
     4141 aacgttaaac gttaatgttg cggtgcggga tcgttatgtg tttcctgcgc cacctcaatc
     4201 gccggtttgt tctgcaatac ggtccagata accagcgcac ccagcaggtc aaatactgcc
     4261 agaactgcga acagcgggct gaagccgatg gtgtcagcca gtgcaccgac aaccagcgca
     4321 aacagcgtac ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg
     4381 ttacgaccga acacgtcaga agagagcgta atcagcgcgc cagacagtgc ctggtgggca
     4441 aaaccaccga tacacagcag cataattgcg acatacgggt tggtgaacag accgatcata
     4501 cccgggccaa tcatcagcac agcacccagc gttacgacca tcttacggga aacgatcagg
     4561 ttcacaccaa accaacgctg gaacagcggc ggcaggtaac caccgaggat acaaccgagg
     4621 tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa
     4681 actttaaaca tgaacagcgg gatccacgcg ttaaaagtac cccaggccgg ttctgccagg
     4741 aaacgcggca gtgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt
     4801 ttcttcgccg tgctcacctg gtgctgggct tcctgaccat taataatata gtcgcgttct
     4861 tcatcggtca gatgcttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc
     4921 cagataaagc tcaatgcacc ggagatgata aatgccatct gccagctgtg catcacgatt
     4981 gccataccac cagcggcggc gcaatcatcg caccaatcga agaacctacg ttaaagtagc
     5041 ctactgcgat ggaacgctct ttcgccggga accattcgga gctggctttc agacccgccg
     5101 ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc aacagccagg ccaccccagc
     5161 tacctgccag cgcggttgca ccacagaaca cggcccacag cacagcaaac attgcataac
     5221 cgattttcgt acccagcaca tccagtacat aacctgctac cggttgcatg accgtataag
     5281 cagcagaata ggctgcgatg atataggaat actgttgggt ggagatgttt aactcttcca
     5341 tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc acggtgccga
     5401 gcgtcaccag tgcgatcata taccaacgta accctttaat tttacgcatg taaaacctca
     5461 tcgttttgtt atatccgcag gaagcgcggc catcatcccg ccagaggctg aagatgttag
     5521 ttacgaccgg gtaaccgaaa aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt
     5581 gctaattcgg cttccgtacc ggttaacccg attcatctaa caaatgaatg aatcggcaat
     5641 ttgcattccg tcgacttatg atttgcgacg gcagaaagat aacttgtcat acaactttaa
     5701 aaggtgagag ccatcacaaa tgtgcgaata tttgtaggga cattacctga cgacagcgag
     5761 gccagtactg gcgcggcctg cagcgagatt tatcactttg agagtaattt tttaactacg
     5821 tttattgatc taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt
     5881 cagcatcgca ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt
     5941 ttatgactga agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg
     6001 caaaagacca gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag
     6061 actatcgttt taaaaacctg tatgacatct ggctgaaagg cgatcactac aaatggcgcg
     6121 ctatgcgtac caacggtgtg gccgagcgtc tgtgtaccgg tgatgcatct gaccgtgaaa
     6181 aatttgacgc ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga
     6241 cgcaccttga actgcgtcgt ccgtttggta tcactggcaa attgctttct ccgtcaactg
     6301 ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg
     6361 gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc
     6421 tggagcatca cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct
     6481 ggcgtccgga caaagccttc aacatcgaac aggcgacctt taacgactac atggcgaagc
     6541 tgggcgaagt ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca
     6601 aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg
     6661 tgatgtttgc tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg
     6721 gcgaaaccct gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg
     6781 gtgccgaata tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata
     6841 acaacctgcg tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc
     6901 gtccgatggc ggaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc
     6961 cgaaaaccat tctctactgc ctgaacccgc gcgataacga agtgctgggc accatgatcg
     7021 gtaacttcta gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta
     7081 acgatcagaa agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtctgctga
     7141 gccgctttgt cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat
     7201 acttccgccg cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg
     7261 cggacatcaa cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact
     7321 acttcgccat tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc
     7381 gctggataac gtcgcggtcg ctttagcaga tttggctgaa ggcacagaag tcagtgtcga
     7441 taaccagact gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga
     7501 tatcgcaaaa ggggccaacg tcatcaaata tggcctgccg attggttatg cattggcgga
     7561 tattgcggcg ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga
     7621 tcagtatcgc tatcaacctg attttcagga tctgcctgcg caagcggcag atcgtgaagt
     7681 gcagatctat cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc
     7741 aaccgtgggc tgtgtcaacg gcatcgcgcg gcaaatccag aaccgtttcc tgaaagagac
     7801 caacaacgcc gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca
     7861 actgggcgac gatcacatca acatccgcac catgctgcaa aacatggtgc gccacccgaa
     7921 cgcgggcgca gtgctggtga ttggtctggg ctgtgaaaac aaccaggttg ccgcattccg
     7981 tgaaacgttg ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga
     8041 tgatgagatc gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga
     8101 taaacgcgag ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga
     8161 cggtctttct ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc
     8221 taacggcggt actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct
     8281 gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt
     8341 taaacagtac tttattgccc atgatcagcc gatctacgaa aacccatcac cggggaacaa
     8401 agcgggcggt atcaccacgc tggaagacaa atcacttggc tgtacccaga aagcgggttc
     8461 cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg aaaacgcctg ggctgaactt
     8521 gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca
     8581 tatggtgctg ttcagtactg gtcgcggcac gccatacggt ggatttgtgc cgacggtgaa
     8641 aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac tggatcgact ttgacgcggg
     8701 tcagctgatc cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt
     8761 tgagtttgcc aacggtaagc aaacttgcaa cgagcgtaac gacttccgcg aactggcgat
     8821 cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc
     8881 ataatgtgaa gcgccgtttg cttttccata cctgataagg atttatcatg accgcgtatt
     8941 ggctggccca gggcgtgggt gtcatcgcct ttctgattgg tatcacaaca tttttcaatc
     9001 gtgacgaacg tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt
     9061 tttttcttct gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat
     9121 tgattacctt acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg
     9181 gaattggcct cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg
     9241 tcagtacctg ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt
     9301 caacgtgttg ctgggtgatt cacaacttct gggcggggtc gataggcggc acaatgattg
     9361 agggtagttt tctgcttatg aatggcttga atatcattcg tttctggcgg atgcagaaaa
     9421 ggggaattga tccgtttaaa gtagagaaaa cccctcccgc cgtagacgaa aggggttaaa
     9481 caattaatta cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc
     9541 agtgaacagc acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc
     9601 gatgataaag ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt
     9661 acaggccagc gggatcagca gcagagaacc ccccgccacg ccggatgcgc cacaggcaca
     9721 cagagaagcc accacgctca acagcagcgc cgtgggcaga tcgaccggga tacccagcgt
     9781 attaaccgca gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt
     9841 ggcacccagc ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag
     9901 tgccatattc accggaatgt tcgccgcaga gctgcgggtg aagaaggcat acacgccgct
     9961 ttcgcgcagg cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag
    10021 caatgggtta accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg
    10081 cgcgtagccc cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagaccaaa
    10141 aataccaatc ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg cattcgacat
    10201 atcgttgacc aggtttttgg tggtctcgtt accgtgacgc agtgcgaaac cgaggccaat
    10261 tgcccacacc aggatcccga tgtagttacc tttcagcagc gcgtcgatgg ggttggaaac
    10321 catgctcatt accagcccgc gcatcacttc tacaatgcct gacggtggcg aaatatcacc
    10381 cgcgctactg gataagtgca gggtagaagg gaaggcgaag ctgaaaacga ctgcggccag
    10441 agcagcagag aaggtaccca gcagatagag gaacaaaata gggcggatat tggttttctg
    10501 cccatgctgg tggttagcaa tagatgccat caccagcatc aacaccagga tgggggcaac
    10561 ggctttcagt gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt
    10621 tgatatccat gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct
    10681 gccatgagcc agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt
    10741 tcattgtgtt atcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa
    10801 agtgtttgca aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat
    10861 tttaaagaga agtcatattt tgaaacttaa tggttacatt tgtttgacat aaacagaggg
    10921 aaggactggc tactaaggct gattagataa catatgggca aaactatttg gaatatgaga
    10981 acggttgcgc tcctttaatg tgctatgcca tataaagtca ctggcgcagg gagcgcgcag
    11041 ggggcggcca atcgccgccg ccccctgcac ccccaggctc tggcgaacaa aatcgccgct
    11101 gcgcgattcc ctcagcttat cccttgcggc taccgggtcg ggcgcaaggg aacgtccctg
    11161 taagatgcgc cctcgccgcg catccatgcg cgtcgccccg gcctccaggg aacgcttcgg
    11221 cgattttgac gccaccaaca cccgtgcggt ctttttgtaa agagctacaa gccgattaca
    11281 taattaatag ctcacctttg tctg
//
LOCUS       DAETQN010000133        11298 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.133, whole genome shotgun sequence.
ACCESSION   DAETQN010000133 DAETQN010000000
VERSION     DAETQN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.133"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..595)
                     /locus_tag="K0W68_003583"
     CDS             complement(<1..595)
                     /locus_tag="K0W68_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075042.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
     gene            complement(1025..1348)
                     /locus_tag="K0W68_003584"
     CDS             complement(1025..1348)
                     /locus_tag="K0W68_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075043.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            complement(1516..1935)
                     /locus_tag="K0W68_003585"
     CDS             complement(1516..1935)
                     /locus_tag="K0W68_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075044.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            complement(1935..2225)
                     /locus_tag="K0W68_003586"
     CDS             complement(1935..2225)
                     /locus_tag="K0W68_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075045.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            complement(2546..2836)
                     /locus_tag="K0W68_003587"
     CDS             complement(2546..2836)
                     /locus_tag="K0W68_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075046.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            complement(2836..3725)
                     /locus_tag="K0W68_003588"
                     /pseudo
     CDS             complement(2836..3725)
                     /locus_tag="K0W68_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4089..4751)
                     /locus_tag="K0W68_003589"
     CDS             complement(4089..4751)
                     /locus_tag="K0W68_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBI3075047.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
     gene            complement(5120..5671)
                     /locus_tag="K0W68_003590"
     CDS             complement(5120..5671)
                     /locus_tag="K0W68_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3075048.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQMLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            6258..6605
                     /locus_tag="K0W68_003591"
     CDS             6258..6605
                     /locus_tag="K0W68_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBI3075049.1"
                     /translation="MILDSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFI
                     GSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEI
                     GAVPAIPQQSSLF"
     gene            6658..7053
                     /locus_tag="K0W68_003592"
     CDS             6658..7053
                     /locus_tag="K0W68_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBI3075050.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            7094..7837
                     /gene="cmoA"
                     /locus_tag="K0W68_003593"
     CDS             7094..7837
                     /gene="cmoA"
                     /locus_tag="K0W68_003593"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBI3075051.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7834..8805
                     /gene="cmoB"
                     /locus_tag="K0W68_003594"
     CDS             7834..8805
                     /gene="cmoB"
                     /locus_tag="K0W68_003594"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBI3075052.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8970..>11298)
                     /gene="torZ"
                     /locus_tag="K0W68_003595"
     CDS             complement(8970..>11298)
                     /gene="torZ"
                     /locus_tag="K0W68_003595"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBI3075053.1"
                     /translation="DGKILTAERWGAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQ
                     VHTTTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPS
                     AIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVY
                     EQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPI
                     RSETIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYL
                     RGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHWML
                     VTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRA
                     IPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEM
                     IVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDF
                     DVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKL
                     IEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAP
                     DEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSIAN
                     GDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSS
                     QLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
BASE COUNT         2909 a         2630 c         2363 g         3396 t
ORIGIN      
        1 tcagcttagc cagtcttgat ttcagcaaat taatctcatt ttctcttgct ttcatcagtt
       61 tctctttggc aatttcttca agctcagctt gtgatttatc agcaacaatt agagacagtc
      121 gatgtggtgc tctgacagct ctttctgtac gaacaactct ttgaccttct ggtaaatctg
      181 caatcacttt accaatctct acagggtcat acccccaacc gtcacggtct agtcgagaga
      241 aataagcata agaattggtt tgaagctcca tcgaaataac catttcgtca atttcgtctt
      301 caagcttgga ctgaattttc tcgtaaacac ttctcattgc ttcttgagag tctttaatag
      361 cttggaaata tcggcgttgt agcttcaact tcaaatcaag ttcacaccgt tcgttcatag
      421 aaaaatcaat ttcaactctt ggaatctcga gtggttctgg tggaatatat ggacctttat
      481 ttacttcgtg tgattcccgc caagctctat caaaagattc acgcaaagtt aattcgtgaa
      541 tatcaagtgc acttggtctg tgttcgataa gcatttcatg ttcatttcta atcattcgtt
      601 caccccttag tttatttgtt atttcaattt atagaagcac actcagcaat cagaggtgaa
      661 gagtctgact gaacaagaac ctgccaagtg tgcttttaaa aactgaaaaa gccctaaaca
      721 tttctgtgaa gggcaaagga ttcaataatg aatgaacaag agtacttttt gagatagcct
      781 ctcagactaa cctgaaaggc catgacgaaa agcattgcac ctttccaact cagaatatcc
      841 atccatcgga ttattgaaag aaaaataaga gagaattgct ctcccataat taaaattatg
      901 ccatgcttaa ggttatttgt caacagcttt ttaacaggtt gataacatca attcttattt
      961 accgcattgt taataataca accaacaaat aagccaagca acgtccaagc aattattaaa
     1021 cccattagtg aaccctcaca gaatccgtca ccagaatatt atcactgtac aggattgtca
     1081 cagcaccttt caaaaattgc tctggggaaa tactgtatat gttcgtttgc tcgataacca
     1141 atcttttcat gttgctgtct gtaattggta gcatcattcg aggctcgtta aactccaccg
     1201 actgaataaa gtcagagatg ttttctacat tatcagccat cagctttagt aatttatcaa
     1261 aattaattgc gatacctgca tcaatgaagc tttgcaccat catagcagtg tcgttatcaa
     1321 ccataatttg cattgggtaa ttattcattt ttaaatctcc tatgaaacac acctcagacc
     1381 tcttaaaata gccattctga ggccagtaac catgcgggtt acagagggag ttgcactggc
     1441 tggtaaggta atttatcaaa ttaaggtgca actctgcctg taaagtgcat tctaccgttt
     1501 cttagatggt tatttttaat tgtctttatt aatcaatggc ttaatgtaga aatactgtct
     1561 ccgtgaaata ccatattttt ctgtaatgta tcgctgagat tgaccagcat ttatggcctg
     1621 agtgatatca tcacggttat agcggttacc acgggattta tatttgtcac catccacatc
     1681 ttctgaggta ttgaagtctt caatcagctt aattgcatcg tgaaggtcat cttctgaggc
     1741 ataatcgaag gtatggataa tctcaaaatc accagtatct gctggtgcat tcttccatct
     1801 ggagaagcgg gattttaatt gctggtgtgt gaagggttca gcattacgag ttaaatcttt
     1861 ccatgttccg ttattctcac ggacaaggtg acctctatgg cgataaaacg catgataatc
     1921 aaatgtgcat agcattaaac ccccagctta tccgccagtt gatgaacctt ggcactcaac
     1981 acctgaatca ttgagcaacc tgccactgcg gactgtgctt tgagtagttc cagttggccc
     2041 gttgtgattc tgatggcctt agaaccctct gcaacgccct tgatagcgtc aaacggtgta
     2101 agggctatgt gttccttcaa aatctcacca acaagtgctg tgtggctcat ggctgggtat
     2161 ttgtctttga ttgctgccag tctggaatat gcctctggca aaaggcgtgt aactgtgtgt
     2221 ctcattaaat tttccttctt aaaatctttg acttataact aactttaaca tatttataac
     2281 atattgtcaa caactcattt acatgttaat atggttgtag ggggctgata caggtcattc
     2341 cctttgataa ataaggggaa ttaatgggct ttcttgaaac aattgctgaa tctcacagat
     2401 tcgcaagctt aggttgcttt gtgtttctgg tgcttcttgt gtatcagctt ttaaaaatga
     2461 ttatcgccag aagtacacga ttcaatttca gcattaagct tttcaaatat gaaatcacaa
     2521 ttacgattga tgatggtaaa acaaactaac gttttgtgat aatattggtg gcctttaagt
     2581 tgctggggtc accgtctttt aatcgtagct ggtggattga attccagata tcaaaatgat
     2641 acagcactgc aaacctcaaa gcgtcaatct ttaaatctgt cacaccatcg taaaagaaat
     2701 aacccctccc ttctttcttt ggtctaatcc ggtaaacttc cccgtgagta tccttcataa
     2761 gatgaccttc acgagaaatg ctgtaacgct ttaacaggta atctttgtat ctcttttcac
     2821 cgtgatattc tttcattact taattcctcc accggcaaaa tacatcttcg gtttaatttt
     2881 ttgcttcttg tcatcctgag tttgtttctt tttcggtgaa agataataat catcttcact
     2941 gtcatcgaat gggtctaaac ccgatacttt gttcttttgt cttgcaagct cgaatctctt
     3001 tttcttctct gccctcttaa tggctttttc acgttcatcc tcttcatcat cgaaggggtc
     3061 caactcatca tcagtggcgg ggcactgtgt gtttttatgg tcactatcaa cactcacatc
     3121 agtgatttct ctgtcttgtt tagaagtctt tttagagctt acttcaaggt cagattcatt
     3181 catagagaga aaatcattta attcatccaa agaaagaaag tcatcttctt taatatcatc
     3241 attaagtcca gtctctttat tcttatctgt aatattatta tctaatgttt tatctaataa
     3301 cttatctaaa ctctaacacg ggtgacattt tgtcactacc cccatgacat tttgtcactg
     3361 gtagtggtgt cattttgtct tactgaggct gcaacttcct gtgatgtagg tcttacaagt
     3421 aattccggct tatcatccag tgtgaataca cgataagtgc tttgacgccc ctgattcttt
     3481 tgcacctgaa ttaaaccgtg tttctcaagt gtctcaatgg ctctgattgc tgttctcttt
     3541 gatacaccag ccatataagc gagatggtca atacttggtg aatatgattt gtatttctct
     3601 tcatagaagt cacagctatg gaccatgacc atataaatca atcttgctgc tgcgttaatt
     3661 tcttctgaaa gttgagagat tcgcacatca aatttataaa actgatttgg gttatattta
     3721 gacattcata aattacctca tacgtgaaaa ggccaaccgg ataaaaccga ttagcctgtg
     3781 tgattaaaat atctggggga ttgatggact gataaagaag tccatgcctg aaacagtacg
     3841 atgattaaaa cgctgaggta taccctgaat gagaggttaa tatacgccaa ccatctgaca
     3901 cctccccaag tgttaccact tcaagacatg aactttttta tcagcccata tagaaactat
     3961 aacagatgcg attataaatt gcaacaatta tttcacagaa tgattactga ttggtcattt
     4021 attgttcaca gggatacttc ttaacatctg aatcacctgt cttgtaaatg accatattta
     4081 tgagaatttt agctcaacct ttgagcaatc ctgtatgacg tgctcagagg cattctgaga
     4141 gcatctgcaa tgtacttaat gggttttcct tgcctgtata gttttatgaa gctgtcgagc
     4201 gtctcaggtg ggtaaatggt gggtcttcct cttagtccac cacggttaat gatggttaac
     4261 ggtagcaatg tatcagcaca gcaaaccatc tgtgtaattt catgcttacg tggttctttt
     4321 ttaaaaacaa cgactgcact tggaaacggt gacctaatag taagcctgtt atgccctgct
     4381 ggtggaaggt atttaactgc acgatgaaga atacggattt catgacagtg gttattgctg
     4441 taaatgtgcc taagccagta gctggtgttg gctctatgcg gtatcaggaa agctactaaa
     4501 tccgcttcag acgcttttaa gaggaacgac ggaatatcgc tgtacggcgg gttacagaag
     4561 accttttcac taacccatga ttgacggctt atatcatcgg tgaatcgtgg taacagcgtg
     4621 tcatgctcag ttgcagcacc atctagactg aaaataaatt cacggtgtaa gttcttaaat
     4681 aacaaaaatg gagttctcca gtaatcagaa cccccatgat gcttcccgat taaaatatgc
     4741 tcaattgaca taagaaataa aaatttttat tgcaatcact cttgacaagg tttactctct
     4801 tttaagagat gcttaacagg ttttctaaac ggatatcctt tctgggatat ctatctttta
     4861 tcttaaaagt ttttacagat actttaaaga tatctgtata tattctgtac agtaactttt
     4921 cagagtctga aaatttttat aaaaaatgac tagccctctt tgaatctcat cttccccgaa
     4981 tcttcgaaaa tatctgtcta gttgattcgg tagaatctac ctcatgatgt atacgtgttt
     5041 agtacccgtc acaccagcta tgtgaccccc ttggcaaaaa agccaaatat cagctacatc
     5101 catgtaacta ttcagtaact tatttagcaa gtctgtaata agttgctcta gagattccta
     5161 gctcttcctg aatctgcaac ctcgttttac cttgtgaaat aagcatctgt acagcctctg
     5221 aagaagcttt tacaggtctt cccatatgta ccccttcagc tttagcacgt tcaataccag
     5281 cctttctacg ctctgctatc aagcttcttt caagctgtgc caacgttgac ataatgccta
     5341 acattgcttc acccatagcg cttgataagt caaatccttc tctaaggctg ataactgtca
     5401 cacctttagc ctgtagcgct ttaaccgttg ataacacatc aattgtgtca cgtccaagac
     5461 ggtctacagc taccacaact acggtgtcac cttctcgtgc ataagctaac aaactgctga
     5521 aaccgtttct taaagaagct ttgacagaac cactgacagc ttcatcagcg aaccatttag
     5581 caatgttgta ccctgcctct tcaatttgca tttgctggtt agtgatggat tggtcatctg
     5641 tactcaccct gcaatatcca agtatcgcca taatgttccc cttagaagtc tcaaaagtta
     5701 gtctcataag cttagctgtc tcataatcca aaaacaacct tttgagacta cttgagggtg
     5761 cattctaccg ttacgttgaa agtgtctcaa aaggacttcc ttatgagact ggttaataga
     5821 gcgctatgaa gattcaggag ggtaactata accatcactt tctaactttc acgtaggaaa
     5881 tcgttaagtt tcttgatagt tacaccatgt gaccaactat ctatatatcg ttatatagcc
     5941 ctgtatatag cactatatgt agtgtctgga gagggacgac taacacgata cagtttaccc
     6001 tgaaaagaga cttcacagat tctttatata tttcaataag ttatgctaaa aagagactat
     6061 accgatactg catagaattt gtgtaggatt gcatagatac ttggaaaaac accgttaaat
     6121 tgcatagtca ttcacctgat attgtcctgt ttttacaggt agttgattcg gatgttctag
     6181 gaatcatcct catagttgat tcggttagtt gggcttgtac caacccctaa tcaacctcat
     6241 agtgtattta gaccttgatg attttagaca gccgccctgt tcatgcagca cgtccacaca
     6301 gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt tccggtacat gctgtactga
     6361 cggcgacaaa tccgctgatt cgttttatcg gtagtgatga tatgacgcaa aaccgggaat
     6421 tatttcaggt ctggttacag aaattagcgc agtggcatca gaccactacg ccttatcttt
     6481 ttttacatac gccagacatt gcccaggccc cggaactggt acataccctg tgggaagact
     6541 tacgtaaaac gcttccagag atcggagcag ttccggctat tccacagcaa tcttctcttt
     6601 tctgaatttg ccacctatca tagacaggtg ccatcggcca ttttaaaggg agtttgtatg
     6661 gtaagcgcgc tatatgccgt tttaagtgcg ttgttattaa tgaagttctc ttttgatgtc
     6721 gttcgcctgc gaatgcagta ccgcgttgcc tatggtgacg gtggttttag cgaactgcaa
     6781 agcgctattc gcattcatgg taacgcggtg gaatatattc ccatcgcgat tgtgctgatg
     6841 ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc atatttgcgg catcgttttg
     6901 cttgctggtc gtctgatgca ttattacggt tttcatcacc gtctgtttcg ctggcgacgt
     6961 tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg tgctggcgaa tctttggtat
     7021 atgccctggg agttggtttt ctccctgcgt tagcgcacaa tacgccactt tctttttccc
     7081 ggatttttac gttatgtctc accgcgacac gctattttct gcccctatcg ccagactggg
     7141 cgactggacc tttgatgaac gggtagctga agtcttcccg gatatgatcc agcgttccgt
     7201 tcccggctat tccaatatta tttccatgat tggtatgtta gccgagcgct tcgttcaacc
     7261 tggtacgcag gtttacgatc tgggttgttc tctgggcgcg gcgacgctct cggtgcgtcg
     7321 caacattcat catgataatt gcaaaattat tgccatcgac aactccccgg cgatgattga
     7381 acgctgcagt cgtcatattg acgcctataa agcccctacg ccagtagacg tcattgaagg
     7441 tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg gtgctgaatt ttaccctgca
     7501 attcctggaa ccttccgagc gccaggcatt actggataaa atttatcaag gactgaaccc
     7561 gggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa gatgccaaag ttggtgaact
     7621 gttgttcaac atgcaccacg actttaaacg tgccaacggt tacagcgaac tggagatcag
     7681 ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat tccgtggaaa cccataaagc
     7741 acgcctgcat aaagccggtt ttgagcatag cgagctgtgg ttccagtgct ttaactttgg
     7801 ttcactggtg gcattaaaag cagaggacgc tgcatgatcg actttggtaa cttttattct
     7861 ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc tgcccgcgca gattgctaac
     7921 tggcagcgcg agcagcagca cgggctgttt aagcagtggt ccaacgcggt ggaatttctg
     7981 cctgaaatta aaccgtatcg tctggattta ttgcatagcg taaccgccga aagcgaagag
     8041 ccactgagca ccgggcaaat taaacgcatt gaaacgctta tgcggaacct gatgccatgg
     8101 cgcaaagggc cgttctcact gtatggcgtc aacatcgata ccgaatggcg ttccgactgg
     8161 aaatgggatc gtgttctgcc ccatctttct gatttaaccg gacgcaccat tctggatgtc
     8221 ggctgcggca gcggttatca catgtggcgc atgattggcg caggcgcgca cctcgcggtg
     8281 gggatcgacc ctacgcaact gtttctgtgc cagtttgaag cggtgcgtaa actactgggt
     8341 aacgatcagc gcgcacattt gttaccgtta ggtattgaac agcttccggc actgaaagcc
     8401 tttgataccg tcttttcgat gggcgtgctt taccatcgcc gttcgccgct ggagcatctc
     8461 tggcagttga aagatcaact ggtgaatgag ggtgaactgg tgctggaaac gctggttatt
     8521 gatggcgatg agaacacggt gctggtacca ggcgatcgtt acgcgcaaat gcgtaatgtc
     8581 tatttcatcc cttccgcgct ggcgctgaaa aactggctga agaagtgtgg ttttgtagat
     8641 atccgcattg tggatgtgtg cgttaccact actgaagagc agcgacgcac tgaatggatg
     8701 gttaccgaat ctctgtccga tttcctcgac ccacatgatc cgagtaagac agttgaaggt
     8761 tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc cgtaaaggtc tggtattact
     8821 gccggatgcg gcgtgaatgc cttatccggc ctacaaaccc ttgctaattc aatctattgc
     8881 agggactatg taggcctgat aagcatagcg catcaggcag ttttacgttt gcataacctc
     8941 agcgcccgtt tccgggcgct attcacttct tacgcctgaa cagcaggctt atcaaatgcc
     9001 gttaacttcg gtgcgttacc cgtatatttt tcgatataca ccagcgcaga gttaccggca
     9061 caggcatttg ccagctgcga gctggggata tccgccgtta acacgttcgc actgccgttt
     9121 ttacacaagc cgttttccag atctggccat gcaccttcat gcacgcatac cacgcctttt
     9181 ttgatcccgt cagtcaccac cgcgcctgtc aaaatctgac cacgtttgtt ccagacacgc
     9241 accagatcgc cattcgcaat actaaatcga gcagcatctt cggtgtgaat agtgatcggt
     9301 tcacgatcgg caaccgcata ttttttacgc agttccgcat agttaagctg actgtgtaaa
     9361 cggtgtgccg gatgtgcggt cagaagctgc aactgcttct cgtcggcggt acccttccac
     9421 tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct tatagccaaa tttttccagc
     9481 gttttggagt aaatctcaat tttgccactt ggcgtaccca gcgcattttt caccggatcg
     9541 gcgcggaaat caccataacg aacgtactgt tcgttcttct cgctgcggcg catttcgatc
     9601 agtttatttt gctgccagaa ggcattaaac attggcatag tgacgcgttg cgcacgggcc
     9661 cctttctgag cggcatcata gaaaaatttc agccacgcca tttcatcttt accttcggta
     9721 tagatctctt ttccgccagg tttgagtaat tccgcaagat cggcaaacac atcaaaatcg
     9781 tttcgcgctt caaattgcgg agcaacagcc tgcttcatcg gcacaatatg ctggttgctg
     9841 taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg tggtgatcgg taatacgata
     9901 tctgcgtgtt tagcagctgc ggtccagtag cattcagaaa cgacgatcat ctccggtttc
     9961 tgccatgctt taatcagacg attagtatcc tggtgatggg taaagttacc gccacccgcc
    10021 caccagatca ttttgatatt cggatacgtt tgctctttac cgttatgctg atatttgccg
    10081 ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa tcgccctcat tccgccatca
    10141 tcagcggcct cgctggcctg tccggcaatc gccgcagaca tttccggcag cacgccgcca
    10201 acacgtgtcg ggttgccacc attggagtaa tgataagaga aaccaaaacc gccgcccggt
    10261 gtaccaatct gccctaacat cgccgccagc gtcaccagca tccagtgttt ctgttcacca
    10321 tattgctggc gctgaattcc ccagcctgcc atcagcattg tgcgattcgc cgccatcagt
    10381 tcagcgagtt tgactatctg cgcttccggt acgccagtaa tttccgccgc ccaggccgcg
    10441 ctcttcggcg tattatcgct cttacctctc agatactctt caaactgcgg atacccggta
    10501 gtgtattttt caaggaacac tttatcgtgt ttgccttgtg tcatcagggt atgcgcaatc
    10561 cctaacatca gtgccacgtc ggtgcccata ttcggcgcga tccaggtggc attatcgcca
    10621 aagaagtcga tggtttcgga gcggatagga tcaatggcaa tcactggttt gccagatttt
    10681 ttcagctgat ggaagtattc cagcccttgc tcatcggtac tgctccaggc aatttttaag
    10741 gtattcagcg ggttcatccc ccacagcacc acaacctggc tgttttccag aatcagcggc
    10801 caggaggtct gctgttcata cacctctaca gaaccgacca catgcggcat gatcacctgt
    10861 gccgccccgg ttgaataatc gccgctatgc ccggaataac cgcccgccag gttcatataa
    10921 cgttgcagta aggtttgcgc tttatgcaac acgccagaag agcgccagcc gtatgatccg
    10981 gcaaaaatag ccgatggtcc gttagctttg cgaatgcgat cgtgttgctc atgaatcagt
    11041 tttaatgctt gttcccagct cacctgtaca taggtatctt cgccacgacc tttcgtcggt
    11101 tgcagtggat tatcgagata gctttttctc accatcggat gctgaatacg cgtcgtggtg
    11161 tgtacctgat ccgccgccgt agactgtaag gaattcggta tggttttcgc cagcgcgcct
    11221 gttgaagaaa caatcttgcc gtccttcact tctacgttca tcgctcccca acgttccgcg
    11281 gtgaggattt taccgtcc
//
LOCUS       DAETQN010000134        11242 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.134, whole genome shotgun sequence.
ACCESSION   DAETQN010000134 DAETQN010000000
VERSION     DAETQN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.134"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..308
                     /locus_tag="K0W68_003596"
     CDS             <1..308
                     /locus_tag="K0W68_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBI3075054.1"
                     /translation="VLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFF
                     DQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            507..788
                     /gene="ppiC"
                     /locus_tag="K0W68_003597"
     CDS             507..788
                     /gene="ppiC"
                     /locus_tag="K0W68_003597"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBI3075055.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="K0W68_003598"
     CDS             complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="K0W68_003598"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBI3075056.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2500..3393
                     /gene="ilvY"
                     /locus_tag="K0W68_003599"
     CDS             2500..3393
                     /gene="ilvY"
                     /locus_tag="K0W68_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBI3075057.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="K0W68_003600"
     CDS             complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="K0W68_003600"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBI3075058.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="K0W68_003601"
     CDS             complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="K0W68_003601"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBI3075059.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="K0W68_003602"
     CDS             complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="K0W68_003602"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBI3075060.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="K0W68_003603"
     CDS             complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="K0W68_003603"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBI3075061.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8116..9762)
                     /locus_tag="K0W68_003604"
     CDS             complement(8116..9762)
                     /locus_tag="K0W68_003604"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBI3075062.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9765..9815)
                     /locus_tag="K0W68_003605"
     CDS             complement(9765..9815)
                     /locus_tag="K0W68_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075063.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="K0W68_003606"
     CDS             complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="K0W68_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBI3075064.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10353..11242
                     /locus_tag="K0W68_003607"
                     /pseudo
     CDS             10353..11242
                     /locus_tag="K0W68_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT         2554 a         3090 c         2950 g         2648 t
ORIGIN      
        1 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
       61 gcgatcatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
      121 acctgcaatt ctttgatcag cagattcgtt gggccaaagc cgacctagta caacaagtca
      181 gtcgaaaccg attaaacaga gcaaggactt acagagggta tcttaatcaa tgtttgccac
      241 atgagttggt tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atatcgctca
      301 taaattgaca ataaaataac catccccgat aatgactttg tagcccatag aggccctaag
      361 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac
      421 cagcccctcc ctgactaaca attacgttag catctgcgga tcatgctaaa atcgccgcct
      481 gacaattatt cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
      541 taaaagaaga gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg
      601 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
      661 aattccgcca gggtcagatg gttccggcgt ttgataaagt tgtgttctct tgtccggtac
      721 tggagccaac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
      781 gtaactaata gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt
      841 gggagagggt cggggtgagg gcatcagcgc gcacttaacc cgcaactgca atacgtttca
      901 tatccgtcat atagccgcgc agtttcttac ctacctgctc aatcgcatgg cagcgaatcg
      961 cttcgttcac atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
     1021 gatcgcccgg ttgcagctct gccataaacg gtttcagcag cggcacacaa gcgtaagaga
     1081 acagatagtt accgtactcg gcggtatcag agataaccac gttcatttcg tacagacgct
     1141 tacgggcgat ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt
     1201 cttcgatgat gccggaatca accatggttt caaacgccag ctcaacgccc gctttcacca
     1261 tcgcgatcat cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
     1321 gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
     1381 catcgttggc ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca
     1441 tatgtttctg gaacaggggt gccatgatct ctttcagctg ttcagacagc gcgtaagcac
     1501 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg
     1561 cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc
     1621 cctcttccac cagcttgtcg aagcacagca gagagccagc ctgcaacata ccgcacagga
     1681 tggtttgctc acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc
     1741 ccgcacggtg accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt
     1801 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
     1861 cttcacgtac ttcggtgcca gggcatttcg gcgcaaccat gactacggta atgtctttac
     1921 ggatctgctc gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt
     1981 ctttcatcag tggctgtacg gtgcgcacta catcagagtg ctgcttgtct ggcgtcaggt
     2041 taaccaccag atccgcctgc gggatcagtt cttcgtaagt accaacttta aaaccgtttt
     2101 cggtcgcttt acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
     2161 agatatcgag accagaatca cgcatgttca ggccctggtt cagcccctgt gcgccacagc
     2221 cgacgataac cactttttta ccctgaaggt agctcgcgcc gtcggcgaat tcgtcgcgcc
     2281 ccataaagcg acatttgccc agctgtgcca gctgctggcg caggttcagt gtattgaagt
     2341 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga
     2401 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac
     2461 attgcaattt ctgcaacgtc aacatcgagg gctgcgccat tggatttacg cgatctgaaa
     2521 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
     2581 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta
     2641 tttgtgcgcg ataaccgcac ggtaacgctg acggaagcgg gcgaagagct gcgcgttttc
     2701 gcccagcaaa cgctgttgca gtatcagcaa ttgcgccaca ccatcgatca gcaagggccg
     2761 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg
     2821 ccgattctgg atcgcttccg cgcggaacac ccgtcggtgg agatcaaact caccactggt
     2881 gatgcggcag atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg
     2941 aaaccggaga cgctacccgg cgctgtggcg ttttcgatgc tggagaatct ggcagtagtg
     3001 ctgattgccc ccgcgctgcc ctgcccggtg cgtaatcagg tatctgtcga aaagcccgac
     3061 tggtcaacgg tgccgtttat tatggccgat caggggccgg tacgccgccg cattgaactg
     3121 tggtttcgac gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa
     3181 gcgatggtat cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtggtgctg
     3241 gaaaacagcc cggagcctgt acgcaaccgg gtaatgattt tagatcgcag cgatgagaaa
     3301 acgccgtttg aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag
     3361 gcgttctgga ggattttgcc gaaccacaaa tgacgttgtc gcgcgggtag gcctgataag
     3421 cgaagcgcta tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccggattatt
     3481 ggtttcgtcg tggcaatcgt agcccagctc atttagccgg gtttcgaaat ccggttcatg
     3541 gtcgccgagt tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg
     3601 gaacaaagaa atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
     3661 tgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc
     3721 gaccatatag cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta
     3781 gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg
     3841 cacgccgaca aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac
     3901 cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt
     3961 caccgccaac aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
     4021 gccgtggaag ttcacgttgg caccggaaag aatatgcgcc aggcgttcgc cgcgaatgtt
     4081 gtgctgagcg atatattttt tcattcccgc cagcgccaac gcgccagagg gttccgccac
     4141 tgcgcgcaca tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt
     4201 gatgatgtcg tcgagatact cctggcataa acggaaggtt tcgtcgccga tgcgttttac
     4261 cgcgacacct tcggcgaaca gtcccacgcg cggcagatca accggatgac ccgcatccag
     4321 cgctgctttc aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgtggcat
     4381 cagttgtttg atcagcaccg caacgcccgc cgccagaccg ccgccgccga ctggcacaaa
     4441 tacgcggtcg agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc
     4501 aatcaccatc ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc
     4561 gatcgcttta gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa
     4621 tccacgtacc gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt
     4681 cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt
     4741 gatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg
     4801 cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag
     4861 acgcgacgac agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg
     4921 cgcgcgcagc actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga
     4981 gtcagccatt attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg
     5041 gttgccaggc tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc
     5101 gtccaggctt tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg
     5161 cttacctgta actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca
     5221 atcagaccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca
     5281 gaggtgccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt
     5341 gatttcagga agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct
     5401 tcatagcgga tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc
     5461 gcgtcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg
     5521 acgcccgctg ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg
     5581 ccgccgtctt tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca
     5641 tcgagagaat cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg
     5701 cctgcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
     5761 agagtttgcg gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg
     5821 cgatccagtt caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
     5881 tatttttggg tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg
     5941 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc
     6001 gtgttggtag agccgcccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg
     6061 ctagcaatat tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat
     6121 tcaacaatgc gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc
     6181 agcgagccgt tgcctggctg cgacaaaccc agcgcttcgg tcaggcagtt cattgagtta
     6241 gcggtaaaca tcccggagca ggaaccgcag gttgggcagg cggaacgttc aacctgatcg
     6301 ctctgggagt cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagc
     6361 ttgatgatct gctcggacag tttggttttc ccggcttcca tcgggccgcc ggaaacaaag
     6421 atcaccggaa tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag
     6481 ttagagatgc agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca
     6541 gcgatcagtt cgcgagatgg cagtgaataa agcatccccc cgtggcccat agcaatccca
     6601 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
     6661 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
     6721 ttcacaaccg cgataatcgg cttgccaaaa tcggcatcgg tcattccggt ggcgcgccac
     6781 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
     6841 atactttatt tactcccagt gtctgtctcg taaatgggac ggtaagtgcc gtcccatttt
     6901 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa
     6961 gaggccgaag aaggcttgct gaatgcattt ggtaaccggg ccgcaacggc cttcacccac
     7021 ctgaataccg tctacactgc gcactggcgt gatttctgcc gccgtaccgg acataaacac
     7081 ttcatccgcc aggtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag
     7141 ctctttcgcc agtttgatga tggcatcacg ggtaataccc ggcagcgcgg atgaggtaaa
     7201 cggtggggtg aacagcacgc cgtctttcac ttcaaacagg ttttcacctg cgccttcaga
     7261 gatgtaaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct
     7321 acccaccagc agggaagaga ggtagttacc accggctttt gccgccgtcg ggatggtgtt
     7381 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
     7441 gcccagatac gctccccacg ggaaagcagc gataatcacg tcggttgagt atcccgctgg
     7501 cgggtttact cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt
     7561 gagattattt ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgcga
     7621 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
     7681 gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaagtgcc
     7741 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
     7801 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
     7861 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
     7921 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
     7981 tatttatatt ttgtgcatcg ctggcggcgg ccatattcat tgagcagacg tggaaaccac
     8041 gatgacgcac cacgcgtaaa acacgttcta aggtttctgg attgaagcga gccgatacat
     8101 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg cgccaggcgg
     8161 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
     8221 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc
     8281 agggatgccg aaggcgctgg ctaacatgag gaaatcgggg ttatcagtaa gggtggtttc
     8341 gctgtatcgt tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt
     8401 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac
     8461 attcatcatg aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac
     8521 ttgtgcgcca accgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat
     8581 gaaattttcc gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac
     8641 atctgtggtc acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg
     8701 cgcgtagata gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc
     8761 gcagtattgc tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt
     8821 taaatcaccc tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat
     8881 atccatatgg ataacactgg cgtgtggtgc gaaagtattc agtttgccgg tcacccggtc
     8941 atcaaaacgt gcgcccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc
     9001 tttgatgccg tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc
     9061 cagccctttc agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc
     9121 cggaactgcc tgcgccatac ccacgccacc gccaacgtac agcatcggtt tttgcgcttt
     9181 tgccagcatc tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac
     9241 ggtggtgaac cacggttcca ggtcaccgct ggctaactgg atatcttttg ggatatcgac
     9301 cagaaccgga ccaggacgac ctgagcaggc aacgtcgaat gcttcagcca tgatgcgcgg
     9361 caactcttcc agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcctag
     9421 gacatccact tcctgaaatg cgtcagtgcc gataaacggt gcggacactt gaccggtgat
     9481 ggcaacaaca gggatggaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc
     9541 gcccggacca gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat
     9601 agccgccatt gccgcaccct gctcatgtcg gcatagcaag tgctccacgc cgccgtcata
     9661 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
     9721 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
     9781 aatcttgaga aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta
     9841 aggtcaaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
     9901 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
     9961 taatcacgac taggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    10021 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg
    10081 gcctattttt atgatagctt taataatttc aatagaagtt gttgtttttt aaaaagaaaa
    10141 tagtgagtga aaaaaattca atttcagtat aaatgttgtt cccttcctcg tagttcatcc
    10201 tttgtccgct gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc
    10261 acaaataaac aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc
    10321 ttatgcacat aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg
    10381 cagccctggg agtaaatgcg ccccctgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg cgcataacgg cg
//
LOCUS       DAETQN010000135        11138 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.135, whole genome shotgun sequence.
ACCESSION   DAETQN010000135 DAETQN010000000
VERSION     DAETQN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11138
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.135"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            57..983
                     /locus_tag="K0W68_003608"
     CDS             57..983
                     /locus_tag="K0W68_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3075065.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(933..2126)
                     /locus_tag="K0W68_003609"
     CDS             complement(933..2126)
                     /locus_tag="K0W68_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3075066.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2262..3986
                     /gene="iucA"
                     /locus_tag="K0W68_003610"
     CDS             2262..3986
                     /gene="iucA"
                     /locus_tag="K0W68_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HBI3075067.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3987..4934
                     /gene="iucB"
                     /locus_tag="K0W68_003611"
     CDS             3987..4934
                     /gene="iucB"
                     /locus_tag="K0W68_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HBI3075068.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4934..6676
                     /gene="iucC"
                     /locus_tag="K0W68_003612"
     CDS             4934..6676
                     /gene="iucC"
                     /locus_tag="K0W68_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HBI3075069.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6673..8010
                     /gene="iucD"
                     /locus_tag="K0W68_003613"
     CDS             6673..8010
                     /gene="iucD"
                     /locus_tag="K0W68_003613"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HBI3075070.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8016..10211
                     /gene="iutA"
                     /locus_tag="K0W68_003614"
     CDS             8016..10211
                     /gene="iutA"
                     /locus_tag="K0W68_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HBI3075071.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDFLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10762..10905)
                     /locus_tag="K0W68_003615"
     CDS             complement(10762..10905)
                     /locus_tag="K0W68_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075072.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2799 a         2787 c         2881 g         2671 t
ORIGIN      
        1 cacaagttga aataatagta ttatattgaa aagacagaga ttatttcaga ggtgttatgg
       61 aaaactgggt tattcaggag ctaaaatcac tggatatagg ggactcaaga cttgataaaa
      121 gagtaaaaca tatcctcaat agcctcagtc gttcaccaga agaaagtatt cctgtttcct
      181 gccgtacctg gagtgaaaca aaggcagctt atcgttgttt ttcaaacgat aaagtctctg
      241 ctaataaaat tatggctcct cacaaagaaa atatcactga aagagctcag cagtttaaaa
      301 gagttttagt tttacaggat acaacagaac ttaattattc tggtcagaaa gaaaaacaag
      361 gagttggtca caaaaaacat aaagatgaaa gaaacttatt tcttcatcct caactcgtca
      421 tcagtgagtc aggtgtttgc ctcggtgttt atgatgacta tcagtggttc agggatgaat
      481 taaaaaccag taaaaatacc cgacaagaaa tctgcaatga tttacttcat aaaaaacatg
      541 tcagtgaaaa agaaacctgg cgttgggttg aaggatataa taaagcaaca gaactggctc
      601 gccaatgccc tgatacccat gtattgtcaa tttctgatcg tgaaggcgat ttttatgacc
      661 tgtttgaacg cgcagagcag actcccggaa taaaagctga ctggcttgtc agaatgaaat
      721 ttaataacag agcaacgctg aatattaatg gtaaacggga tcataggttg ttgcacgaaa
      781 gaatcatgga gattacaccg cagcaactgg ttgaattcac tatacctgat gggcgggggc
      841 agcaagccag aacactgcct gacatgcttt catcagacgc aggtcctctc tcatatcctc
      901 tgatttactc cagtaaggtg atgatcatat ctttatttga tagtacggta atgccagata
      961 aatacagaca caatggtaaa taatatagtt gcaaataatg ctgccataat cagcgcgatt
     1021 gcgacacctg ttttcgatac cagcggcacc agcaaagcgc acagtccata gccaagtgtg
     1081 tgactggtag cgagccagcc cgcgcctgcg ccatcagcca gcttatcatt cagtagaagt
     1141 tgataagcgg gtgttgccag agctgctcca aatgacagca ctgcacagcc cgggtaaaac
     1201 aaccatagct gttcggagag catgatagcc agaccaccac tcatcaacac tccggcactc
     1261 aggagcaggg ccaccggagt caggcgctgc ggacggagta ccccgaactg cgcgataagc
     1321 gcagctactg cggacagacc caacagccag gccacctgtt ggctaatggc ggtggtatcc
     1381 gtgacgaact ggcgagtaag ggcaggcgaa agtccaagct gcatcatgct caccgccgca
     1441 gccagtaata ttgcgcaaag cagataaggc agacaatccc gcttcagact gacgctcttg
     1501 cactccggtg tgggctgtgg tggtgtgccg ggcaaccgca gcagcatcag cagcgccagc
     1561 actggtgctg ccatcagtag ccccagtggc gccagaggat gaatggccag cattgctgcc
     1621 gcgcacagcg gcccgaatag cctgccgcaa ctcaggccgg agctgatggt tgccagagcg
     1681 gccatgcgat tcccttctcc agctctctgc aatgcccaga cctgacaggc tggcaccatt
     1741 gccgacacgg tcagcccgta ggcgatccgg gcgatgatta atattcccag tcccaccgct
     1801 gttgttatgg cgctggtagc catcagcacg cttcccagcc ctagcagggt aaagcttatg
     1861 ccatatcctc ccagagccca caacaccaca ggtttactgc cagtacggga aatttgcttt
     1921 ccccaccacg gggacgatgg cagaaacagc attgagccaa gcatcagcag ggcagcccat
     1981 acagacagac tcagatttgt ctgtatgacc agaacaggga gcacaaccag cagaccgttc
     2041 tgtccgatac cgagaagccc ggcactgaac gcaagtggcc agcaggacag tgttttttgg
     2101 ggcgtatctt cgatccctgg tgacattata atgtttcaac tccatgtatt aattgtgttt
     2161 atttgtaaaa ttaatttatc tgacaataac atttattgtt gataatgaga atcattatca
     2221 acataattgt tattatttta ctgtgtggga gctgtttgac tatgaccctg ccctctgaaa
     2281 aaccagccac agatgtggct gcgcagtgct tcctgaatgc actgattcgt gaaaccacag
     2341 actggaaact gacagaatac ccgccagacg aattgctcat cccgctggat gagcagaaat
     2401 cgctccattt cagagtggct tatttctccc caacccagca tcaccgcttt gcatttcctg
     2461 cacgtctggt cacggcatca ggcagttatc ctgtcgactt taccactctc tcccggctta
     2521 ttattgataa gctacgccat caactgtttc tgcccgttcc cctctgcgaa actttccacc
     2581 agcgcgtgct ggaaagccat gtccatacgc aacaggcaat tgatgcccgt catgactggg
     2641 ccgctctgcg tgaaaaagcg ttgaattttg gcgaggctga gcaggcgctg ctgacaggac
     2701 acgctttcca ccctgcgcct aagtctcatg aaccgtttaa ccgacgggag gctgaacgct
     2761 acctgcctga tatggcaccc cacttcccac tgcggtggtt ttcggtggat aaaacgcaaa
     2821 tcgccggtga aagtttgcat ctcaaccttc aacagcggct gacgcgattt gccgcagaga
     2881 atgcgcctca gttactcaac gaattaagtg acaaccaatg gctgttcccg ttgcacccgt
     2941 ggcaggggga atatcttttg cagcaggggt ggtgccaggc acttgttgct aaagggctga
     3001 ttaaagactt aggtgaggcc ggcacgtcgt ggctgccgac cacctcttcc cgttccctct
     3061 actgtgccac cagccgcgat atgatcaagt tctccctgag cgtacggctg accaactcca
     3121 tccgtactct gtccgtgaaa gaagtgaagc gtgggatgcg cctggcacgc ctggctcaaa
     3181 ccgacggctg gcagatgcta caggtccgct tcccgacttt ccgggtaatg caggaggatg
     3241 gctgggccgg gctgctcgat cttaacggca acatcatgca ggaaagtctg tttgccctgc
     3301 gtgaaaatct gctggtggat cagccgaaaa gccagactaa cgtactggtc tccctgactc
     3361 aggccgcacc tgatggcggt gattcgctgc tggtttcggc ggtaaaacgc ctgagcgatc
     3421 gcctcggtat cactgtgcaa caggccgccc atgcatgggt cgatgcatac tgtcagcaag
     3481 tgctaaagcc gctgtttacg gctgaagcgg attacggcct ggtgctgctg gcgcatcagc
     3541 aaaatattct tgtccagatg cttggggatc tgccggtcgg atttatttac cgtgactgtc
     3601 agggcagcgc ttttatgcct cacgcgacag actggctcga ttctattggc gaggcgcagg
     3661 cggaaaatat tttcacccat gagcagttgc tgcgctattt cccttattac ctgctggtta
     3721 actccacttt tgctgtgacc gctgcgctgg gggctgccgg gctggacagc gaatcgaatc
     3781 tgatggctcg tgtacgagca tcgctggctg aagtgcgtga tcaggtgact cataaaacct
     3841 gtctcaacta cgtgctggaa agtccgtact ggaacgtaaa aggtaacttt ttctgttatc
     3901 tgaacgatca taacgagaac accattgttg acccttcggt gatctatttc gatttcgcta
     3961 acccgctgca ggctcaggag gtctgaatgt ctgaggcaaa cattattcac agcagatatg
     4021 gactgcgctg tgaaaaactc gacaagcccc tgaatcttgg ctggggactg gacaatagcg
     4081 cggtgttgca ctgtcccggg gagctgccga cagggtggct gtgcgatgcg ctggatcaga
     4141 tatttatcgc cgcaccacaa ctgtcagcag tggctctccc ctgggccgaa tggcgtgagg
     4201 agccacaggc gctgacgctt tttgggcagg taaaaagcga cattatccat cgtactgcct
     4261 tctggcagtt accgttatgg ttgagttcac cggcaaaccg ggcctccggt gaaatggttt
     4321 ttgatgcaga gcgtgagatt tatttcccgc agcgcccacc ccgtccacag ggtgaggttt
     4381 accgccgtta cgatccacgt attcgcagga tgctgagttt ccgcattgcc gatcccgttt
     4441 ctgatgcaga acgtttcacc cgctggatga atgatccgcg cgttgagtat ttctgggagc
     4501 aaagtggctc actggaggta cagaccgcct atctggagcg ccagctcacc ggtaaacatg
     4561 cattcccgct gattgggtgc ttcgacgatc ggccattcag ctatttcgaa atctactggg
     4621 cggcggaaga ccgcattggt cgtcactatt cgtggcagcc ctttgaccgt ggcctgcatc
     4681 tgctggttgg tgaacagcaa tggcgcgggg cccactatgt gcaaagctgg ctgcgcgggc
     4741 tgacacatta cctgctgctg gatgagcccc gtacgcagcg cacagtactg gagccacgca
     4801 ccgataacca gcgcctgttc cgccatcttg agcctgcggg ataccggaca attaaagagt
     4861 tcgacttccc acataagcgc tcgcgcatgg tgatggcgga tcgtcatcac ttcttcacgg
     4921 aggtcggtct gtaatgaatc acaaggactg ggattttgtt aaccgtcggc tggtggcaaa
     4981 aatgttgtct gagatggagt atgagcaggt tttccacgcg gaatctcagg gcgatgacca
     5041 ctactgcatt aacctgccgg gagcacaatg gcgcttcatc gctgaacgtg gtatctgggg
     5101 ctggctgtgg attgatgccc aaacgttgcg ctgcacggac gaaccagtac tggcgcagac
     5161 gctgctgatg cagctaaagc cggtgctgtc aatgagtgat gccactgttg cagagcatat
     5221 gcaggatttg tatgccacgc tactgggcga cctgcaactg ctgaaggccc gtcgcggact
     5281 gagtgccagt gacctgatta atcttgatgc cgatcgtctg caatgcctgc tgagcggtca
     5341 tcctaaattc gtttttaata aaggtcgccg tggctggggc aaagaggcgc tggaacgata
     5401 tgcgccagag tataccaaca ccttcagact gcactggctg gcggtaaaac gtgaacatat
     5461 gatctggcgc tgcgataacg atctggatat tcagcagttg ttgactgccg caatggatcc
     5521 gcaggagttt acacgcttca gtcaggtctg gcaggaaaac gggctggatc acaactggct
     5581 accgctgccg gtacatccgt ggcagtggca gcaaaaaatc gccacagact tcatcgctga
     5641 ttttgtcgaa ggcaggatgg tgtctctcgg cgagtttggc gaccagtggc tcgcccagca
     5701 gtcgctgcgt accctgacca acgccagccg ccggggaggg ctggatatca agctgccgct
     5761 gaccatctac aacacctcat gctaccgggg aatccctggc agatatatcg ctgccggacc
     5821 actggcttca cgctggctgc aacaggtttt tgcgaccgac gccaccctgg tgcaaagcgg
     5881 cgcagtgatc cttggtgaac cggctgcagg ctatgtgtcc catgaaggct atgccgcgct
     5941 tgcccgggct ccctatcgct accaggaaat gcttggtgtt atctggcggg agaatccgtg
     6001 ccgctggctg aaaccggatg aaagtcctgt tctgatggca acactgatgg agtgcgacga
     6061 aaacaatcag ccgctggcag gcgcatatat cgaccgctcc ggactggacg ctgaaacctg
     6121 gcttacgcaa ttgttccggg tggttgtggt tccgctgtat cacctgcttt gccgctacgg
     6181 tgtcgcgctt attgcacacg gacaaaatat aacgctcgcc atgaaagagg gggtaccaca
     6241 gcgtgttctg ctgaaagact tccagggcga tatgcggctg gtgaaagaag cgtttcccga
     6301 aatggactct ttgcctcagg aggtgcgtga tgccacatcc cgtctgagtg cggactactt
     6361 aatccatgat ttgcagacgg gtcacttcgt gacagtactg cgttttattt cgccactgat
     6421 ggttcgtctt ggcgtacctg aaaggcgatt ttatcaactg ctggcagcag tgttgagtga
     6481 ttacatgaac aaacatccac aaatggcaga gcgttttgcg cttttctcac tcttcaggcc
     6541 acaaatcatt cgcgtggtgc tgaacccggt aaaactgacc tggccggacc tggatggcgg
     6601 cagtcgcatg ctgccgaatt accttgagaa tctgcaaaat ccgctgtggc tggtaactca
     6661 ggaatatgaa tcatgaaaaa aagtgtcgat tttattggtg tcgggacagg gccatttaat
     6721 ctcagcattg ctgcattgtc acatcagatc gaagaactga actgtctctt ctttgacgaa
     6781 catcctcatt tttcctggca tccgggtatg ctggtaccgg attgtcatat gcagaccgtc
     6841 tttctgaaag atctggtcag tgcagttgca cctacaaatc cctacagctt tgttaactat
     6901 ctggtgaagc acaaaaagtt ctatcgcttc cttacaagca ggctacgtac agtatcccgt
     6961 gaagagtttt ctgactatct ccgctgggct gctgaagata tgaataacct gtatttcagt
     7021 cataccgttg aaaatattga tttcgacaaa aaaagtcgat tgtttctggt tcagaccagt
     7081 cggggagaat attttgcccg caatatctgc cttggtacag gaaaacaacc ttatttacca
     7141 ccctgtgtga agcatgtgac acaatcctgt ttccatgcca gtgaaatgaa tcttcgtcgg
     7201 ccggacctga gtggaaaacg gataaccgtg gttggtggag gacagagtgg tgcagacctg
     7261 ttccttaatg cattacgcgg ggaatgggga gaagcggcgg aaataaactg ggtctcacgg
     7321 cgtaataatt ttaacgcact ggatgaggct gcttttgctg atgagtattt tacacctgaa
     7381 tatatttcag gcttctccgg actgaaggaa gatattcgcc atcagttact ggatgagcag
     7441 aaaatgacat cggatggcat cactgccgat tctttactga ccatttatcg tgagttgtac
     7501 caccgttttg aagttctgag aaaaccaaga aatatccgtc tgctacccag tcgctcggta
     7561 acaaccctgg aaagtagtgg tccgggctgg aggttattga tggaacatca tctggatcag
     7621 ggcagagaga gcctggaaag tgatgtggtg attttcgcca caggttaccg ttctgcattg
     7681 ccacaaatac ttccctcact gatgcccttg atcaccatgc acgataagaa cacctttaaa
     7741 gtgcgtgatg acttcactct ggaatggagt ggcccgaaag aaaataacat ctttgcggtc
     7801 aacgccagca tgcaaactca tggcatcgcc gaaccccagc tcagcctgat ggcctggcga
     7861 tctgcacgta ttcttaatcg cgtactgggg cgtgatttat tcgatctcag tatgccgccc
     7921 gcactgattc agtggcgcag cggcagccgg aaaaaaccgc agccggaggc tgcttcttta
     7981 actcactata cagcaaatat tcaggaataa cgatgatgcg caaaaagtat atgccccggg
     8041 ctcttggtcc gctgcttctt gtcgtgctgt caccagctgt cgcccagcaa aacgatgata
     8101 atgagatcat agtgtctgcc agccgcagca atcgaactgt agcggagatg gcgcaaacca
     8161 cctgggttat cgaaaatgcc gaactggagc agcagattca gggcggtaaa gagctgaaag
     8221 acgcactggc tcagttaatc cccggccttg atgtcagcag ccagagtcga accaactacg
     8281 gtatgaacat gcgtggccgc ccgctggttg tcctgattga cggtgtgcgc ctcaactctt
     8341 cacgttccga cagccgacaa ctggactctg tcgatccttt taatatcgac catattgaag
     8401 tgatctccgg cgcgacggcc ctgtacggtg gcgggagtac cggagggttg atcaacatcg
     8461 tgaccaaaaa aggtcagccg gaaaccatga tggagtttga ggctggcaca aaaagtggct
     8521 ttaacagcag taaagatcac gatgagcgca ttgccggtgc tgtctccggc ggaaatgacc
     8581 atatctccgg acgtctttcc gtggcatatc agaaatttgg cggctggttt gacggtaacg
     8641 gcgatgccac cctgcttgat aacacccaga ccggcctgca gcactccaat cggctggaca
     8701 tcatgggaac cggtacgctg aacatcgatg aatcccggca gcttcaactg ataacgcagt
     8761 actataaaag tcagggagac gacaattacg ggcttaatct cgggaaaggc ttttccgcca
     8821 tcagcgggag cagcacacca tacgtcagta aggggctgaa ttctgaccgc attcccggca
     8881 ctgagcggca tttgatcagc ctgcagtact ctgacagtga tttcctggga caggaactgg
     8941 tcggtcaggt ttactaccgc gatgagtcgt tgcggtacta cccgttcccg acggtaaatg
     9001 cgaataaaca ggcgacagct ttctcctcgt cacagcagga taccgaccag tacggcatga
     9061 aactgactct gaacagccaa ctgatggacg gctggcaaat cacctggggg ctggatgctg
     9121 agcatgagcg ctttacctcc aaccagatgt tcttcgatct ggctcaggca agtgcttccg
     9181 gagggctgaa caaccataag atttacacca ccgggcgcta tccgtcatat gacatcacca
     9241 atctggcggc cttcctgcaa tccagctatg acattaatga tatttttacc gttagcggtg
     9301 gcgtacgcta tcagtatact gagaacaggg tagatgattt catcgactac acgcagcaac
     9361 agaagattgc tgccgggaag gcgatatctg ccgacgccat tcctggtggt tcggtagatt
     9421 acgataactt tctgttcaat gctggtctgc tgatgcacat caccgaacgt cagcaggcat
     9481 ggttcaattt ttcccagggg gtggcattgc cggatccggg gaaatattat ggtcgcggca
     9541 tctatggtgc agcagtgaac ggccatcttt ccctgacaaa gagcgtgaac gtcagcgaca
     9601 gtaagctgga aggcgtgaaa gtcgattctt atgaactggg ctggcgcttt accggtgaca
     9661 acctgcggac tcaaatcgcg gcatattact cgctttccaa taagagcgtg gaaaggaata
     9721 aagatctgac catcagtgtg aaggacgaca ggcgccgtat ttacggcgtg gaaggtgcgg
     9781 tggacttcct gatcccggat actgactgga gtaccggtgt gaacttcaat gtgctgaaaa
     9841 ccgagtcgaa agtgaacggt caatggcaaa aatatgacgt gaaggaatca agtccatcga
     9901 aagcgacagc ttacattaac tgggcgccgg aaccgtggag tctgcgtgta cagagcacca
     9961 cttctttcga cgtaagcgat gcagagggta acgatattaa tggttacact accgtcgatt
    10021 ttatcagtag ttggcagctt ccggtgggaa cactcagctt cagcgttgag aacctcttcg
    10081 accgtgacta taccactgtc tggggacagc gtgcacctct gtactactgc ccgggttacg
    10141 gccctgcttc actgtacgac tacaaaggcc ggggccgaac ctttggtctg aactactcag
    10201 tgctgttctg acggggatgc ttttgcaaca agggtatgct gtaaacaaca tggtccttgg
    10261 tggccatgtt gtttacagcc agtataatat acagccttaa caagatattc cgtctccgga
    10321 atggactgtt caccactgaa taacattatt tctgttttat ggatctttga ctgaaattta
    10381 tcgcatatcc tcatttcatt gtccgtctga aaactaacct tacaagtaag cgtcgtctca
    10441 gcaccgtctg gcagatcctg aaattcctga gaggatgatc tgcgcccaat atcctgagcc
    10501 ggaactttgc tgcaatgaag gccaacgaaa aatgggggag tatgttatag agtacttgct
    10561 acagggtaaa aagaggtacc tgtcgctggt tctccgttca gtttaaccgg gagattttct
    10621 cctgcgcctg tcataaagtc cggtaatgga gattgttaat acgatggtgc acgatgcatt
    10681 gtcggtgccc ggcccggagg atgccccgta actacatacg gatcagggtt ggcaatatcg
    10741 aatggcaggc tataggcaaa gttaaagccc catggagtag cacaaaatat gccacacaaa
    10801 ggaaacggtc tgaataacgc agtgatggag aacttcttcg gcacactgca aaatcgcatc
    10861 gtggtcgatc gctaattcgc cgtgaatcac tgaacgatgt tccatgcgaa cagatattcg
    10921 atcatatcga aatattaaac attacccctc cccgcattgg gcaaaaaaaa aacaaaaaac
    10981 gatcaattag tattgattca cataatctct catcattaat gagaacattg tcacatattt
    11041 acaatataga actgttactt ttagtaagct gttagtaacc aactgttact aataatttta
    11101 attcttaata ctttgttcat tcaataaatg agttgaga
//
LOCUS       DAETQN010000136        11003 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.137, whole genome shotgun sequence.
ACCESSION   DAETQN010000136 DAETQN010000000
VERSION     DAETQN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.137"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..278)
                     /locus_tag="K0W68_003616"
     CDS             complement(<1..278)
                     /locus_tag="K0W68_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3075073.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSR"
     gene            complement(445..1185)
                     /locus_tag="K0W68_003617"
     CDS             complement(445..1185)
                     /locus_tag="K0W68_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBI3075074.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1278..1913)
                     /gene="argO"
                     /locus_tag="K0W68_003618"
     CDS             complement(1278..1913)
                     /gene="argO"
                     /locus_tag="K0W68_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBI3075075.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2052..2912)
                     /gene="mscS"
                     /locus_tag="K0W68_003619"
     CDS             complement(2052..2912)
                     /gene="mscS"
                     /locus_tag="K0W68_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBI3075076.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3270..4349)
                     /gene="fbaA"
                     /locus_tag="K0W68_003620"
     CDS             complement(3270..4349)
                     /gene="fbaA"
                     /locus_tag="K0W68_003620"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBI3075077.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4564..5727)
                     /gene="pgk"
                     /locus_tag="K0W68_003621"
     CDS             complement(4564..5727)
                     /gene="pgk"
                     /locus_tag="K0W68_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBI3075078.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5777..6796)
                     /gene="epd"
                     /locus_tag="K0W68_003622"
     CDS             complement(5777..6796)
                     /gene="epd"
                     /locus_tag="K0W68_003622"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBI3075079.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7168..7599
                     /locus_tag="K0W68_003623"
     CDS             7168..7599
                     /locus_tag="K0W68_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBI3075080.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7622..8200
                     /locus_tag="K0W68_003624"
     CDS             7622..8200
                     /locus_tag="K0W68_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBI3075081.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8201..8908
                     /locus_tag="K0W68_003625"
     CDS             8201..8908
                     /locus_tag="K0W68_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBI3075082.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RQAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8896..9573
                     /locus_tag="K0W68_003626"
     CDS             8896..9573
                     /locus_tag="K0W68_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBI3075083.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9567..10244
                     /locus_tag="K0W68_003627"
     CDS             9567..10244
                     /locus_tag="K0W68_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBI3075084.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10216..10929)
                     /locus_tag="K0W68_003628"
     CDS             complement(10216..10929)
                     /locus_tag="K0W68_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBI3075085.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10926..>11003)
                     /locus_tag="K0W68_003629"
     CDS             complement(10926..>11003)
                     /locus_tag="K0W68_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3075086.1"
                     /translation="EARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2627 a         2920 c         2740 g         2716 t
ORIGIN      
        1 cgtgaacgac gccctgagtt atggatttca ttatcaatcg cagaaagtcg ttgataaacc
       61 gggaacagtt tttgatacag ttcctgaccg gctttattga ggctgatatt attatctttg
      121 cgggtaaata gcgtatagcc aatttgttct tccagcgcgg caatgctttt accaaatggc
      181 gaagccgtca tatggatttt ttctgccgcc cgggcaatat tattggtttg cgctaataaa
      241 atgaaattgc gcatcttttt cgaaataaaa atgtccatcc cccctccccg catccttgtc
      301 cgcttaaccg tttcagtcag cctatccttg atgaaaccgc gagcaaagat aggtgattac
      361 gtcatggttt tacagaaaat tacagaaaaa ggaggcaata tcgggtaaag gcattagccc
      421 gacgaatacg tcgggctaca agtattattg tgctgcaggt gttttagcgg gttgttgatc
      481 cacaggttct aactggaaga ccacatcgac ctgatcatca aactgaatag cggcctgctc
      541 gtaagtttcc tgggcagaca ccggcgcggc atcggctttc atcatccgca ccattgggct
      601 gggctgatag ttggaaacat ggtagcgcac gctatatacc ggccccagtt tacgatgaaa
      661 gccgttcgcc agttcctgcg cctgatgaat cgcgttatca atcgctgcct tacgcgcttt
      721 gtctttatag gcatccggct gcgccacgcc cagcgacaca gaacgaattt cgttaagacc
      781 cgccttcagc gcgccatcca gcaaggaatt cagtttgtct aactgacgga gcgtgacttc
      841 caccgttctc acagcgcggt agcctttcag gatactttta ccatcctgat aatcataatc
      901 tggctgggtg cgtaagttcg ctgagctgat atcttttttc gcgatctgat tgagttcaag
      961 gaaggaaatg tattgtgcga cgcgctcatc tgcctgtttc ttggctgtag cggcatcctt
     1021 cgcggccacg ttaacttcaa tcgcaagagt ggcaatgtct ggcaccgcat ccacacttgc
     1081 cgtaccggag gtgacaatat gcggtccatc cggcaattcg ttagcctgcg ctgccatccc
     1141 gctaataccc attaatgccg ccagggcgat aactttgaac ttcacgttag tctctccgtg
     1201 ttaattgact tgcccaaaat tcaggacctc tggaggcaag cttagcgcct ctgttttatt
     1261 tttccatcag atagtgccta actgaacaag gcttgtgcat gagcaatacc gtctctcgcc
     1321 agctgcaagg ctataaacca catgacacat cccactacca gattgataat gcgctgtgat
     1381 tttgccgtgc gcaggcgcgg tgccagccag gctgcgagaa tagccagacc aaagaaccac
     1441 aggaaagagg cgctaattgt cccgagcgca aaccaacgtt ttggttcgac gtcaagctgc
     1501 ccgccaaggc tgcccagtac aacaaaagta tccaggtaaa catgcggatt cagccaggtc
     1561 actgccagca tggtggcgat aattttccat ctgccttgct tcaggacttc ggcactagct
     1621 aactcaatat tactactcat cgctgtttta aaagcgccaa aaccatacca cagcaagaag
     1681 gctacgccgc cccaggtgac cagcgccagc aaccacggcg actgcatcaa taacgcgctg
     1741 ccaccaaaaa tcccggcgca aatcaggacc aaatcgctga tagcacaaag taaggcaatc
     1801 ataatgtggt actgacgacg aatgccctga ttcatcacaa aagcattttg tggaccgagc
     1861 ggcagaatca tagctgcccc aagagcaata ccttgaaagt aataagaaaa cacgttggat
     1921 attccgaatt aagtatctga tgcagagact atatcgcggg attgttatta gcggaaattg
     1981 ataatattaa ttggcaatca gaataactaa taagagagag gccagagagt attatctggc
     2041 ctcagcgttg attacgcagc tttgtcttct ttcacccgct taaagttcac atccatttgc
     2101 gggtacggga agctgatacc ggcggcatca aattcacgtt taatacgctc cagcacatcc
     2161 cagtacacgt tttgcagatc gccgctgttg ctccagacgc ggaccacgaa attaatcgac
     2221 gatgcaccaa gttcgttcag acgcacagtc atttcgcgat ctttcaaaat gcgatcttca
     2281 gactggataa tattggtcag gatctgctta acctgatcga tatcggaatc atacgccacg
     2341 ccaataataa attcgttacg gcgaactggc tcgcgagaga agttaataat atttccggca
     2401 ataattttac cgttcggaat aacgataatt ttaccgtctg cagtacgcat ggtggtggag
     2461 aaaatctgca cactcagcac agtaccggct acgccgccga gatcaacata ttctccggca
     2521 cggaacgggc ggaacatgac aagtaacacg ccagcggcca ggttagaaag tgacccctgc
     2581 aaagccagac caacagctaa gcctgcggca ccgagtacag caatgactga cgctgtttgt
     2641 acacccacgc gtcccagggc agcgattagc gtaaaggcga taataccgta acggactaat
     2701 gcagaaagaa aatcagcaac agtggcatcg attttacggg agatcatcag gcgattcacc
     2761 gcgttggaaa tcatccgcgc gataatcaaa ccaacgatga tgatcgcgag tgccgccacg
     2821 atgtttactg cataacttag cagcagcgcc tggttagcta ccagccagct tcccgcgccg
     2881 tttatgctat cgacaacatt caaatcttcc attcaatatt ccttttcaac tgactcaaaa
     2941 tggagaaata cactccgccg ccttatgacg ggcagtctga caagggtaaa caataaagca
     3001 taattttgcc aaatagatca cagatttctc atctgaatca ccttgctttt ggcgaaaaaa
     3061 tccccgcaga gcggggctca gacagatatc aatgtgcgct ttgtcatgcc ggatgcggcg
     3121 tgaacgcctt atccggccta caaaaacctg caaattcaat atattgcagg atacgtgtag
     3181 gcctgataag cgtagcgcat caggcaattt tgcttttggc gaaaaaaaga cccgcagagc
     3241 gggccttgag ataagcagaa aggaatatct tacagaacgt cgatcgcgtt cagttcctgg
     3301 aatgcttttt ccagacgagc gatcatcgaa gtctgaccgg cacgcagcca tacgcgcgga
     3361 tcgtagtatt tcttgttcgg ctgatcttcg cctttcgggt tacccaactg cccctgcaga
     3421 taagcttcgt tcgctttgta gtagttcaga acgccttccc aggttgccca ttgggtatcg
     3481 gtatcgatgt tcatttttac tacgccgtag cttacggagt ctttgatttc ctgagcagta
     3541 gaaccggaac caccgtggaa tacgaagttc aggctgttgt gcggcaggtt gtgtttcttg
     3601 gaaacatatt cctgagaatc acgcaggatg gtcggagtca gaaccacgtt acccggcttg
     3661 taaacaccgt gtacgttacc gaaggacgca gcgatggtga aacgcgggct gattttgctc
     3721 agttcggtgt atgcgtaatc aacgtcttcc ggctgggtgt acagtgcaga agcgtccatg
     3781 tggctgttgt ccacgccgtc ttcttcacca ccggtgcaac ccagttcgat ttccagagtc
     3841 atgccgattt tggacatgcg ctccaggtat ttagagcaga tttcgatgtt ctcttgcaga
     3901 gattcttcag acaggtcgat catgtgagaa gagaacagcg gcttaccggt agctgcgaag
     3961 tgtttttcac ccgcgtccag cagaccgtcg atccacggca gcagtttctt cgcgcagtgg
     4021 tcagtgtgca ggataaccgg aacaccataa tgttcagcca tctggtgaac gtgatgcgca
     4081 ccagagatcg cgcccaggat agcagcaccc tgcggaacgt cagatttcac gcctttacca
     4141 gcgataaagg aagcaccacc gttggagaac tgaacgataa ccggcgcttt aactttagca
     4201 gcggtttcca gtacggcgtt gatggagtca gtaccgacgc agtttactgc tggcagtgcg
     4261 aagttgtttt cttttgctac ctggaaaact ttctgtacgt catcaccagt gattacgcca
     4321 ggttttacga aatcaaaaat cttagacatg tctcttgtcc tgtatcgtcg ggccttatac
     4381 tcatcatact tcaagttgca tgtgctgcgt ctgcgttcgc tcaccccagt cacttactta
     4441 tgtaagctcc tggggattca ctctcttgtc gccttcctgc aactcgaatt atttagagta
     4501 tgaaaaaatg cgtgctctaa aagcgcgctg aaacaagggc aggtttccct gccctgtgat
     4561 tttttacttc ttagcgcgct cttcgagcat cgctactgca ggcagtactt taccttccac
     4621 gaattcgagg aatgcgccgc cgccagtgga gatgtaggag attttgtcag caatgccgaa
     4681 caggtcgatt gctgccagag tgtcgccgcc gccagcgatg gagaacgctt cgctgtctgc
     4741 gatagcgtta gccacgattt cagtaccttt gcggaagttc gggaattcga acacgccaac
     4801 cggaccgttc cacagaatgg ttttcgcatt cttcaggatt tcagccagtt cctgagcgga
     4861 agcatcaccg atatccagga tctgctcgtc agctttcaca tcgttaacag atttcagggt
     4921 agccgatgca gtttcagaga actcggttgc tacgcgaaca tcagacggaa ccgggatgtt
     4981 gcaggtggtc agcagacgtt tagcttcgtc aaccaggtca gcttcgtaca gggatttacc
     5041 cacatcgtgg ccttgtgccg cgataaaggt gttagcgata ccaccaccaa caatcagctg
     5101 gtcagcgatt ttagacaggg agtccagaac ggtcagtttg gtagatactt tagaaccacc
     5161 aacgatagcc accatcgggc gagcaggttc tttcagtgct ttacccagcg cgtccagttc
     5221 agctgccaac agcgggcctg cgcacgcaac atcagcgaat ttaccgatac cgtgagtaga
     5281 agcctgcgcg cggtgagcag taccgaatgc gtccattacg aacacgtcac acagtgcagc
     5341 gtattttttg gacagggttt cgtcgtcttt cttctcgcct ttgttgaagc gaacgttttc
     5401 cagaacaacc agttcacctt cagcaacgtc aacgccgtcg aggtaatctt taaccagacg
     5461 aaccgggtta gacagtttgt ctttcaggta gttaacaacc ggcagcagag agaattcttc
     5521 gttgtactcg ccttcggtag gacgacccag gtgggaagtt accatcactt tcgcgccttg
     5581 tttcagggcc agttcgatgg tcggcagaga agcacggata cgcgcgtcgc tggttacttt
     5641 cccgtctttt actggtacgt tcagatccgc acggataaat acacgtttcc cagcaagatc
     5701 cagatcggtc atcttaatta cagacatggt gaatcctctc gttgattcta aaagttttgc
     5761 agacgctgct tgcgtcttac ctgaaagcaa cagtagccat agctaacgtc gtgtcgagca
     5821 ttcggttagc aaagccccat tcgttatcgc accagaccaa cgttttgatc aggtgtgcgc
     5881 cactgacccg ggtttgggtg ccatcgacaa tggcactgtg cggatcgtgg ttaaaatcta
     5941 cagagaccaa cggcaattcc gtatagtcaa ctataccatg aaatgcacct tgtgctgctt
     6001 tttgcagcaa caggttgact tcattggctt ttacaggttt cttcaccgtc acgcttaaat
     6061 cgattgccgt cacatttatg gttggcacac gtaccgcaat cgcttcaaag cgatcgttaa
     6121 attgcggaaa aaatcgtgtg ataccggcgg ccagtttagt atcgaccgga atgatcgact
     6181 ggctggctgc ccgggtgcga cgcaggtcag gatggtatgc atcaataacc tgttgatcgt
     6241 gcatggcgga gtgaattgtg gtcacagtgc cggactcaat accgtacgca tcatctaaca
     6301 atttgatgac gggaattatg caattcgtgg tacaggaagc gttagaaacg atgcggtgtt
     6361 ccgcacgaag ttgatcctga ttgacgccgt aaacaacggt cgcgtcgaga tcgttactgc
     6421 caggatgtga aaagagcact tttttggccc cggcggcaat atgcgcttcg ccatgctcgc
     6481 gggagccata tacgccggtg cagtcgagga ctacatcaac gccaagttca cgccagggga
     6541 gcgattgcag tgaacgttca tgcaatacgc ggatggcgtc atcaccaaca aaaagttgat
     6601 cgcgttcctg tcgtacttcc catgcaaaac ggccatggct ggtgtcatat ttcaacaaat
     6661 gcgccatgcc cgcagcatcc gccagttcgt tgattgccac cacggtaatt tccgcccggc
     6721 gtccggattc atacaaagca cgaaccacat tacgcccgat gcgaccgaag ccatttatcg
     6781 ctacgcgtac ggtcatagat ctcctgcaag gttttccctg agcaaatttg ccagacagag
     6841 taatccagca aatcgtccgg ggaaacctta cctgtcgcaa actgcgactg attggttaat
     6901 tgtcgaacat ttaatcgact gaaacgcttc agctaggata agcgaaacgt ggaataaaag
     6961 gaatgattgt ccagtcaaag aaaacaatta tctgacttac gtcacgtttt cagccccgcc
     7021 cctgtggaat ttattccact ttgcagaatc acgaataagc gtagcaaaat cttttcgttg
     7081 tgccgtcaca aaccctgact tatatcagaa tatgactgat gcagcgtcga tattatccgc
     7141 aatacgtccg agtttcggag tactccaatg atgacattgc cctacccata ttcttccccc
     7201 gcacgctttt atgcactgcg cctcctcccc gggcaggaag ttttttccca gctacacgct
     7261 tttgcacagc agcatcaact tcatgccgcc tggattgcag gctgtaccgg tagcctgact
     7321 gacgtcgcct tgcgctatgc cgggcaagaa ggcaccacgc tccttaacgg tacattcgaa
     7381 gtgatctcac taaacggcac gctggaacag agtggtgagc atcttcatct ctgcgtttcc
     7441 gatccacacg gcacgatgct tggcggtcat atgatgcccg gctgtaccgt gcggaccacg
     7501 cttgaactgg tgatcggtag ccttgaggaa ctggcgttta gccgccaacc ttgcgcgctt
     7561 tctggttacg acgagttgca tatttccccc gttaaataac tactttcaaa gaggttttct
     7621 tatggcgcgt agccactttt ccagtcaggc actggtgttg atcgttatat ctatcgccat
     7681 caatatgatc ggcgggcaac tggccagcat ggtcaaatta cctatatttc tcgactccat
     7741 cggcacactt attagcgcgg tactgcttgg tccggtgatc ggaatgctga ccggcttact
     7801 gaccaattta ctctggggat tgctgaccga cccgatcgcc gccgcatttg cacccgtcgc
     7861 gatggtcatc ggcctggtcg ccggttggct ggcgcgagcg ggttggtttc gcaccctgcc
     7921 aaaagtcgtt gtcagcggcg tgattattac gcttgcggta acggtcgtcg ctgtaccgct
     7981 gcgtactgcg ctgttcggcg gtgtcaccgg tagcggagcc gatttgttcg ttgcctggat
     8041 gcactcgatg gggcaaaacc tggtggagtc ggtggcgatt acagtgatag gcgcgaacct
     8101 ggtcgataaa attcttacag cggtgattgt ctggctttta ctgcgccagc tgccgattcg
     8161 caccacgcgc cattttccgg caatggctgc cgtgcgctaa atgcatccat ttacctcatt
     8221 aacactgtgg gcattggcgg cctgcaccac gcttatcctg cccgcgcaga ccattttgcc
     8281 gatctatagc gcggcgacct ttttttgtct tattgcgcta aaggccacgc ggcggcaggc
     8341 aaaatatgtc gcctggctga tgttctcgct gggagccggg ctgtggctgg tacacggtgg
     8401 ctggctaaca gaatggctaa gcggcacgcc gcgttcgccg gagcgttgga cgcatgctat
     8461 tacgctctgg ttgcggattc tggcgattgt ttcaacctca cagttgtgga tgcagtacgt
     8521 ccccgtgcaa tgttttatcc gcgctctgtt tgcttctcgc ctgccgccag gcgtcgctta
     8581 tctgtttgcc ggaccgttat tggtcgttga gcagttaaaa cggcaattag cgattattca
     8641 tgaagcgcag cgtgcccgcg gcgtgccgct ggatgagggc tggtatcagc gtttacgtgc
     8701 gatgcccgcg ttaattattc tgctaacaca caatgcgctt aacgatctgg ctgtccgtgg
     8761 tgcggcgctg gatatgcggg catttcggat ccacaaccga cgaacaacgt tgtgggcacc
     8821 agccgatagc accctacaac gcgtagcgcg ttacaccatg atcttgctga tgctaacaga
     8881 attcggagcg tggatatggt tacgttagaa cagttccgtt attgcccgac acactcaacg
     8941 catcctccgt tctgttatga ctttcactat gtcaaaccgg ggatggtcgc catctttggt
     9001 gacaacggca gtggcaaaag tacgctggca cagctgatgg cgggctggta tccggacttt
     9061 ctgccaggag agataacagg cacggggacg ctcctcggta cgcccattgg gcatctcccg
     9121 cttaatgaac aatccgccac catccagctc gtccagcaat cgccctattt gcagctctcc
     9181 ggctgtacct ttagcgtgga agaagaagtt gcctttgggc cagaaaatct gtgtcttgct
     9241 gaaacagaga ttatggcgcg gattgatgcc gccctgactc tgaccgagtg ccagccgttg
     9301 cgccatcgtc acccggcaac actctccggt ggagagacac aacgcgtggt catcgcctgc
     9361 gccatcgcga tgcagccaaa gctcttaatt cttgatgaag cctttagtcg cctgacgccg
     9421 caggccagcg aaatgctgtt gcagcgctta caacactggg cgcttgaacg tggttcgctg
     9481 attattcttt ttgaacgtca tcgcacacct ttcctgaact actgccagca agcgtggcaa
     9541 ttgcaaaacg gagcgctcca gccactatgt taacgttaaa tcagatatct tatcgctggc
     9601 ctagcgcagc aacggattgt ctttgcgaca tttctctgca actcaaacaa ggtgaatggt
     9661 tggcgctgac cggcgacaat ggcgcgggaa aatcgacgct gttacgagta atggcaggtc
     9721 ttcttacgcc cacagccggt acggttatgc tgcaacaaca ggcgatgaaa aacctgaaaa
     9781 accgccagcg cgcggcaaag gttggcgtac tgtttcagga agcggaaaac caactttttc
     9841 acagcaccgt cgctgacgag atcgcatttg gtttgaaact gcaaaaatgc ccggcggatg
     9901 aaatcactca acgtacccat gctgccctgc aatgttgtca gttagcagac actgccaact
     9961 cgcacccgtt ggatctgcat agtgcgcagc gacggatggt cgccgtcgcc tgcctggaag
    10021 cactctctcc gccactatta ctgctggacg agccgagtcg ggattttgac gaaaattggt
    10081 tgagcgtgtt tgaaagctgg ctggagaagt gccgccagcg aggcacgagc gtggtagcaa
    10141 tcagtcatga cgccgcattt acccggcgtc atttctctcg tgtggtgcga ctggaagatg
    10201 gtgtaatcag gaacgttaat ccaccagacg atattcaccc gtagcggtca tcattaaggt
    10261 caaatttgca ggcagactct cttccagcac ccggcgaaca ttcggcccgt cggtacggtc
    10321 ataaaaggct tctgcatctg ccagcgatac ccctcccgcc agcttgcgcc ccaccagacg
    10381 ctcccgcaac gcagtagctg gcgcgttgat aaagatcgag aaatcacaaa attgcgcaag
    10441 ctggcaccac ttctcgtcgt ccagcaacaa ccagtttcct tcgacgatga cgagtggtgc
    10501 ggtaacgtgc aacgcatctt caacaggatc atgcttttgt cgatcgtact gcggccacgt
    10561 acaatcccct tccaccacct ggcgcagatt ttccgccagt ttcgcaacgt caaatgtctc
    10621 tggtgcgcct ttgaaggggc gcaattgatg cgcgtccagc cagctattgt aatggtgaaa
    10681 accatccatc gggagcgtct gaatagcggg cagctccggg tcttgttgca cgagatattc
    10741 ccagaaggtg gttagggtag atttacccgt ccccggcgga gcgcaaagaa aaaccaccgt
    10801 acgccgctgc ggatttaccg tctgtaaggc cgccagcata cgcagcaacg gtttatgcac
    10861 gcgttctatc tcttcatcat ggtactgcgc ttgcacgttc agcccattca ccgttaattc
    10921 aattttcacg gcgttaattc ctttaaggtg gtcgttactg ccagcgtcag cgccgttttg
    10981 accattccgc taaaacgcgc ctc
//
LOCUS       DAETQN010000137        10929 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.138, whole genome shotgun sequence.
ACCESSION   DAETQN010000137 DAETQN010000000
VERSION     DAETQN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.138"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..312)
                     /locus_tag="K0W68_003630"
     CDS             complement(<1..312)
                     /locus_tag="K0W68_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBI3075087.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="K0W68_003631"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="K0W68_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBI3075088.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="K0W68_003632"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="K0W68_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBI3075089.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="K0W68_003633"
     CDS             complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="K0W68_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBI3075090.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="K0W68_003634"
     CDS             complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="K0W68_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBI3075091.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="K0W68_003635"
     CDS             complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="K0W68_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBI3075092.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="K0W68_003636"
     CDS             complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="K0W68_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBI3075093.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="K0W68_003637"
     CDS             complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="K0W68_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBI3075094.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8292..9365)
                     /locus_tag="K0W68_003638"
     CDS             complement(8292..9365)
                     /locus_tag="K0W68_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3075095.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9376..>10929)
                     /locus_tag="K0W68_003639"
     CDS             complement(9376..>10929)
                     /locus_tag="K0W68_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI3075096.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
BASE COUNT         2851 a         2830 c         2479 g         2769 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccaaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc caaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atgtttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata aagttatata
     3421 taacaaatcc caataattaa gttattggga tttgtctggt gaattatttg tcgctatctt
     3481 ttcccgccag cagtttatcc agctcatcac cgcctacatg acggaaatcc tgccccttca
     3541 cgtagtagaa gataaactca caaatattct ggcagcggtc gccaatacgt tcgatagaac
     3601 gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg cgaatcttcc atcatgtagg
     3661 tcatcagttg acgaacaata ccttcgtatt cctgatcgac ttttttatct tcacgataaa
     3721 tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg tgcagcatct
     3781 ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc tgctgggaga
     3841 atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc tccagctcgg
     3901 caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc tgacgtttgg
     3961 cgataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg ttcttgtcgc
     4021 cttcgatcac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg attgcatcag
     4081 aaagctgctg ctccaccatg ccacccatgg tcatcacctg cgtgcggata ctttccagtt
     4141 cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata acgcactcct
     4201 gaattaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg gcttggtgaa
     4261 cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg ccgtgtggtc
     4321 ggaacaacgc gcagcctgct gcatgttgtg ggtaacgatc accacggtgt aatcctgctt
     4381 cagttcggtg atcagctctt caatacgccc ggtagagatg gggtcgagcg ccgaacacgg
     4441 ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac acagacgctg
     4501 ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg tttcgttcca
     4561 caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac gggagagctt
     4621 ctcaaacaga cgaacaccaa aagcgatgtt gtcgtagatg gacatcggga acggcgtcgg
     4681 tttctggaac accatgccca ctttcgcacg cagcagtgcg atatcctgag agttggtcag
     4741 gatgttgtcg ccatcaagca gaatttcacc ttctgcgcgc tgctccggat acagttcaaa
     4801 cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc cgataaacgc
     4861 cgttacctgg tttttagcga tatccaggtt gatgtttttc agggcatgga atttgccgta
     4921 gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca tactcattgc
     4981 aatcttttcc tcatcgtacc tgatgctttt cgctaatcag gcttaccgta ggccgggtaa
     5041 ggcgttcacg ccgcatccgg caaagtgtta accgtgttta ttcttcgcaa aaacaacgcg
     5101 cgccagaatg ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag
     5161 ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc
     5221 gatcggctgc atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg
     5281 cgcggtttca ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac
     5341 cgacgctttc agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc
     5401 cgcttcacgc aggctgtccg gcaccagttt cagcatgttc tcggtggtgc ggataacgat
     5461 cggcacctgc aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg
     5521 tgccaccacg atggtgtaga caaacagacc aaccacaatc gacggcacgg agagcagaat
     5581 gtcgttaatg aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata
     5641 aatccccgcc ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc
     5701 gctacccgcc agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc
     5761 agtgaacagc gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca
     5821 gatcagccag aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt
     5881 tttgaggcgg cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg
     5941 catttcaacc atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac
     6001 ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt
     6061 gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt tcgccaacgc agaggtgata
     6121 ctattgcccg gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag
     6181 gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca
     6241 ccatttttgg tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata
     6301 ccgtaggccg actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc
     6361 gcaatgtacg gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca
     6421 aatgcggggc cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc
     6481 ggctcctgaa agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa
     6541 acgatacttg gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag
     6601 ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac tcaccgggac ggcgatcagc
     6661 agcgcgataa acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg
     6721 ttcggtgcat cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc
     6781 caggaggaga tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc
     6841 gccagtttta ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagca
     6901 ggcttggttg cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa
     6961 tgccggatgc ggcgtaaaac accgtacccg gcctggagtt ttattagtac agcggcttac
     7021 cgctactgtc tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg
     7081 gcaggctggc gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga
     7141 agaatttcag cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga
     7201 acgtggtaga ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga
     7261 aggttttgct ccagtctgca ccttttgctg cattagcgaa gttttcttcg gtcggactaa
     7321 ccggtttacc atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag
     7381 catattcaac ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt
     7441 tacctttacc gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt
     7501 tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggtagtc ccggagccat
     7561 ctgcgcggcg tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg
     7621 cgatggtttc atcatcccac ttcttgattt tgcccaggta gatgtcgccg agggttttac
     7681 catccagcac cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc
     7741 caatcacggt cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg
     7801 agtcagaggc accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac
     7861 cgataccctg gtagttaact ttattaccga tttctttctg gtaagtgtca gcccatttgg
     7921 catacaccgg cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag
     7981 agaaagcgct catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt
     8041 tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata
     8101 ggacaaacag gtgacagtta tatgtaagga atatgacatt tttatgacag aaaggaatta
     8161 acaaataact tactgatttt aaatgtgaag atgaaaatcc cacatgtcat ctttttgtca
     8221 tgatgcattt aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt
     8281 tgtcagacct attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc
     8341 ggggctgggc gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat
     8401 ttttcaccat acactgttga tttacccatg tctgttggca actcaccacc gttcactttg
     8461 atttccttat tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc
     8521 attgaattgt ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc
     8581 agtatcccca ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc
     8641 gcacttccaa caacagatga aaaatctgca gctggtattt ctttaaaatc aaaataaact
     8701 ggctgtaaat tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca
     8761 tcagccatcg gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc
     8821 actatctttg tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaaatat
     8881 actatcgctt tacccccact ggatatactt aaaggttcaa tgcggttgca attgtacttt
     8941 gtattaccag acgggttgtt agcatgatat tcaaaaggtg cattcacata cccaacccca
     9001 agaatatcta tagcaatccc aacatcaagg ttcggattaa gatcataata attaatatta
     9061 gagtgaaccc cagcataagt taatgcctgg tttatttttg acgtataata gatatagggg
     9121 aattctccta cattaccagt atcacaatga cagcgtccag gatatgagga tccattagtt
     9181 acagtggtat taaatgtcgt tccaacctta ttttcgttcg cggtaataag atatcccgag
     9241 aaatcaagcg tacggtgaaa tacgccatta tcaggggtac agataccttt aggagctgca
     9301 agtgctacac tacttaacga gagcataaac acagcaaagc accactgttt gatatacttg
     9361 ttcataaaaa tccttttaat gacatgtcac accctgttca attaatccgc tctcactttc
     9421 tggtgtcgaa agtgaataat tgaagatgca ttttgttgag gcgttttctc cccaggataa
     9481 cgtaacctgt cctttttctg gtagaccaga aagataaagt tcgccacggt caccgacaat
     9541 tcctgaatct gccaaacttg catcttgtat cgcagcaatg gctccaaaag gtaccggttt
     9601 accatttgca tttaacactc tgaagagaac gcgataaccc acatgagtca caaactccgc
     9661 tttaaccact gcaccacgcg tgggaaccat cgtaacgacg gtattatcaa gttctatatt
     9721 cgacttcgag aaatagttat cgctgattgc tacccgattt tcatcataag ggtttagctg
     9781 tgtcttgacc gtatagccgc gccagtccgt cgcagcaccg cgcatattat ctatttctaa
     9841 cccggcggcc ccaggtgctt taacaagagc aatagtttca cctaactcct gacttaacgt
     9901 taccccttcg ctgtgtgcca gaattccccc tctgatacca taattcagcc tctgggaatt
     9961 ttggctgtag ttatagctgc cgttcaccgt gccataagag ccatcatagg tcagggacat
    10021 attaccgcca acctcatcat catgactgtt ataggactgc attaaattcc atgaaaggtt
    10081 atttctttcc agcgccgtac cgctgagtcc gagttgatgt gagctaccgg aattatcgtt
    10141 ttgcgaaaca gaataggtgg cgtaactatt accccaccag cccaataaag gaacagataa
    10201 agagagcatg tagatctgct cactggtgtt atcactggca tggttgttcc ccgtcccctg
    10261 tctggcagta ttggaataac tccaggagac gttccacgag agacgcttcc agctactgct
    10321 gtagccaaat tgcattaatc gttcgacatc atcggtatgc cagtattgtt gccggcttaa
    10381 tgtcaggtat aaattgccat aatctctccc caacgactgc gttagcgtcc cctccacgca
    10441 actacgtcgg ttacctgttt cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc
    10501 attgagggta tagtagccct cggtggagta acgatagcca accaagcgca agctagtgtc
    10561 tgtgtcgtca aatcgttttg aatagagaaa gcgatatgac tgacccgttt ctgtatcatc
    10621 atggctaaaa ttcgcacgag catgtgtcac atcgaaagaa attgcgccca aagcgccaag
    10681 atctttaccg acacccagag ctgcagaacc ataattttca gcagcctgca tacctccata
    10741 taaagtgatc ccgtgggaaa aaccatgaag tatttgcgcc tgaaggacgt ccggtgtaaa
    10801 tccgtcttca tcgcgcaatt cacccacgct gacatcaaca tctgtctgac cttcacgttt
    10861 aagaatcgct aatgaagcat aaggttgggt gaatcgttgt tcgctaccgt cttcttcttt
    10921 gatggttac
//
LOCUS       DAETQN010000138        10898 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.139, whole genome shotgun sequence.
ACCESSION   DAETQN010000138 DAETQN010000000
VERSION     DAETQN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10898
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.139"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..611
                     /locus_tag="K0W68_003640"
                     /pseudo
     CDS             1..611
                     /locus_tag="K0W68_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            608..1222
                     /locus_tag="K0W68_003641"
     CDS             608..1222
                     /locus_tag="K0W68_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HBI3075097.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="K0W68_003642"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="K0W68_003642"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBI3075098.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="K0W68_003643"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="K0W68_003643"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBI3075099.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="K0W68_003644"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="K0W68_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBI3075100.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="K0W68_003645"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="K0W68_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBI3075101.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="K0W68_003646"
     CDS             complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="K0W68_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBI3075102.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6632..7735
                     /gene="proB"
                     /locus_tag="K0W68_003647"
     CDS             6632..7735
                     /gene="proB"
                     /locus_tag="K0W68_003647"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBI3075103.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGNSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7747..9000
                     /gene="proA"
                     /locus_tag="K0W68_003648"
     CDS             7747..9000
                     /gene="proA"
                     /locus_tag="K0W68_003648"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBI3075104.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9115..9190
                     /locus_tag="K0W68_003649"
     tRNA            9115..9190
                     /locus_tag="K0W68_003649"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9148..9150,aa:Thr,seq:cgt)
     gene            9796..9954
                     /locus_tag="K0W68_003650"
     CDS             9796..9954
                     /locus_tag="K0W68_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075105.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2760 a         2575 c         2778 g         2785 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc
     3781 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa
     4021 gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcatcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc tctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga agcgcaggaa
     5041 tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagacactga agtggttgaa cgactggagc acaccctacg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat cacatccggc
     5281 aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaccga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact ggcgttatca ctcatataat gcatagcttt aactttgcca
     6241 tagacatcca gtttattacc gtctttatta tatatttctg cagcctgtac agatgcagat
     6301 gccacaatgc ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt
     6361 aattaacgcg cgaatattca gcgggagagc cccgttgaaa acaggaaagt ttttaacctg
     6421 agattgttaa agatatatta cagattaata atattcttaa aatgtggtaa tttattaaat
     6481 ctgtaataaa agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa caaaacgcca
     6541 tgctttgctc gcagatggtt ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat
     6601 catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc tggtggtaaa
     6661 actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc atattgttga
     6721 acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta ttgtgacgtc
     6781 gggcgcgatc gccgccggac gtgagcacct gggttacccg gaactgccag cgactatcgc
     6841 ctcgaaacaa ctgctggcag cggtagggca gagtcgactg attcaactgt gggaacagct
     6901 gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg atatggaaga
     6961 ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt
     7021 tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaagtcg gcgataacga
     7081 taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc tgctgaccga
     7141 tcaaaaaggt ttgtataccg ctgacccgcg cagcaatccg caggcagaac tgattaaaga
     7201 tgtttacggc attgatgacg cactgcgcgc gattgccggt aacagcgttt caggcctcgg
     7261 aactggcggc atgagtacca aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga
     7321 caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg aaggcatttc
     7381 cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac gctggatttt
     7441 cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg gcaactgccg ccattctgga
     7501 acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg
     7561 tgaagtcatc cgcatttgca acctcgaagg ccgcgatatc gcccacggcg tcagtcgtta
     7621 caacagcgat gcattacgcc gtattgccgg acaccactcg caagaaattg atgcaatact
     7681 gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc gttaaggagc
     7741 tggccgatgc tggaacaaat gggcattgct gcgaagcaag cctcgtataa attagcgcaa
     7801 ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa
     7861 agcgaaatca tcctcaacgc taacgcccag gatgttgctg acgcgcgagc caatggcctt
     7921 agcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg cattgccgac
     7981 gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc aggtaatcga tggcggcgta
     8041 ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt
     8101 tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat
     8161 gcggtgatcc tgcgcggtgg caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg
     8221 attcaggacg ccctgaaatc ctgcggctta ccggcgggtg ccgtgcaggc gattgataat
     8281 cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga catgctgatc
     8341 ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat cccggtgatc
     8401 acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgtagagat cgctgaagca
     8461 ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac ggttgaaacg
     8521 ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa acaaatggcg
     8581 gaaagcggcg tgacattaca cgcagatgca gctgcactgg cgcagttgca ggcaggccct
     8641 gcgaaggtgg ttgctgttaa agccgaagag tatgacgatg agtttctgtc attagatttg
     8701 aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga acacggcaca
     8761 caacactccg atgcgatcct gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa
     8821 gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt
     8881 ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg
     8941 ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat tcgtgcgtaa
     9001 ataaaaccgg gtgatgcaaa agtagccatt tgattcataa ggccattgac gcatcgcccg
     9061 gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt cagggccgat
     9121 atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt
     9181 atcggcacca ttaagaaaat taataactta gcttaacttc tctaagaaat tgtatcttct
     9241 gttatcactt acaaacgcct atacgttgtt atgggctttg gtagtgtaac acgtatgggc
     9301 gagtagttcc caggctcaac ctcgtaacaa gttcctttga agctggaaga cgtgccaccg
     9361 tctctaaacc ttctaccaaa actcttagca agatgttcca cacctggcgc ggtgtccaat
     9421 gatgctacac gtcgtgagac gttgcagagg ataaacaggc taaatgatgg gaagagtttt
     9481 aagtgcaata gtggattaaa gcccacctct tcaaaaccta acttgatcgg gaaagaatca
     9541 agctctagca cggtttcgtc caccggagta caacgggtgc ctgataagag aagaactgct
     9601 aagacttcat agcttttgtg aaaaagccct ttttagtgga tacgcctacg gcgcttattt
     9661 tttggtataa ccatcaaagg cgctttatgg ctcctattgt cttgaaaatg tatctttact
     9721 tgtattgtgt tttatgcgta gtctccctgt gtagtatgta acttgttaat tttcatatga
     9781 atacagaggc acacgatgaa aaacattgca gcaatcaaac gtaataaccg caagattcac
     9841 gctcgtaagt tcctgtctac gccagaagga aaagcctggc tagagcgtaa acagagagag
     9901 aacgaagaaa gaaaactcct tagtgagttg aaatggctta gagaggattt ctgaaaatct
     9961 ccagaacgca acaggacgca ttctaacgct gttttcatta ctcggtatag ggataggtga
    10021 tgtgtgttta atgcgtctgt gcgcttgttt tgtgccttat tttgcatttt cataatgcgg
    10081 aagagaacac ggaaaacctt aaaattaatt attggggtat ttaaggctat tttcaggtga
    10141 tttaagggta ttaaacttga tttaattgat taaaatataa acagataatt atgtatctta
    10201 ttgatttata aggttgtttt taagtaaagg taaataaaaa ataaatagcc ataaatagct
    10261 ataaatacat cttaatacct atatcccttc ccttatggct agattgcgag aggtgcttaa
    10321 cgcgattttc agcgcccccc gatctggaaa atccagaatc cccaaatatc agcaaggaat
    10381 tttcttgcgt aatcaacatc gtttttacct attaaatcat tacacagaaa gcctttctgt
    10441 aatgtctctc actaaacttt cactaaacac gagaatgatc ggttttatgt cggatcagat
    10501 gctagaaaca gcaccacgtc tcacaagggc tgtaagcgat gaaaccagtg tttatgcggg
    10561 tgcaggtcaa aatataggcc aaaatccatt taacataata aatgttatat gtgctaagga
    10621 acacatataa cgccttgaat tgatgtatca gtggaaaagt gatggatttt ttacgtagga
    10681 gcacgaaagg gcttctattg acttttgtgt gctatgaaat ataattaaag ttttttgttt
    10741 ttattgtgtg atgtggtggg ttacacaggg gaatagatta aatggtgttc tgtttataca
    10801 ggtttatttg ttagatcaac aatttggcat ttctaaacag atgtttgaac gttgccaaat
    10861 gtcttgacaa cacaagaaaa gggcttgcta tgccaggc
//
LOCUS       DAETQN010000139        10862 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.140, whole genome shotgun sequence.
ACCESSION   DAETQN010000139 DAETQN010000000
VERSION     DAETQN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10862
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.140"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(174..959)
                     /locus_tag="K0W68_003651"
                     /pseudo
     CDS             complement(174..959)
                     /locus_tag="K0W68_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1132..2010
                     /gene="yihU"
                     /locus_tag="K0W68_003652"
     CDS             1132..2010
                     /gene="yihU"
                     /locus_tag="K0W68_003652"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBI3075106.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            2035..2922
                     /locus_tag="K0W68_003653"
     CDS             2035..2922
                     /locus_tag="K0W68_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBI3075107.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2955..3965
                     /locus_tag="K0W68_003654"
     CDS             2955..3965
                     /locus_tag="K0W68_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBI3075108.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            4035..5468
                     /locus_tag="K0W68_003655"
     CDS             4035..5468
                     /locus_tag="K0W68_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3075109.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5567..6916
                     /locus_tag="K0W68_003656"
     CDS             5567..6916
                     /locus_tag="K0W68_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBI3075110.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6906..7793
                     /locus_tag="K0W68_003657"
     CDS             6906..7793
                     /locus_tag="K0W68_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBI3075111.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7934..8533
                     /gene="yihX"
                     /locus_tag="K0W68_003658"
     CDS             7934..8533
                     /gene="yihX"
                     /locus_tag="K0W68_003658"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBI3075112.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8527..9399
                     /locus_tag="K0W68_003659"
     CDS             8527..9399
                     /locus_tag="K0W68_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBI3075113.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9396..9833
                     /gene="dtd"
                     /locus_tag="K0W68_003660"
     CDS             9396..9833
                     /gene="dtd"
                     /locus_tag="K0W68_003660"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBI3075114.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9878..10819
                     /gene="fabY"
                     /locus_tag="K0W68_003661"
                     /gene_synonym="yiiD"
     CDS             9878..10819
                     /gene="fabY"
                     /locus_tag="K0W68_003661"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBI3075115.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2513 a         2575 c         2899 g         2875 t
ORIGIN      
        1 cggggtcgag gtaatccccc agagaagtca gtgggttacg cagagaatcg taacgggata
       61 ccagatcaag agcctgtcca atgtgcataa aaaaatccgg aaacaagtga gcgtttccgg
      121 attcttacac agccactgga tcggtcaact gatccttaac tgatcggcat tacctctttt
      181 gttggcattt tttctgccaa cgctaccgcg agtgcgccgt gaaaaacatc gcctgctcca
      241 gtggtatcaa ccacattgac agaaaatgcc tcctgttgac acaaatgacc atcttcaatc
      301 cacaacgatc cttcagaacc cagcgtaaca tacactttac ctgctgtttg tgtcgttgcc
      361 tggaacaaac cttcttcagg tgactgcagg ccagtcatgc gtttcagccc tggtgttgag
      421 aacacggcgt gatcggcaag cgcgacaagt ggagaaatat cctgcggtgt catatcggcg
      481 tctaaaagcg ttgtcacacc cgcaaggcgc gctagcgaga atgctttttc agtaccgcta
      541 tgccagcgaa catcggccag aatcagatca taacgagaga aatcgatcgc ttctaaccat
      601 tcggcatcag taccgagatc aggacttgga taattaacga taatccgttc gccatgctga
      661 tcaacgagga tcgctgattg cgacgaacgg gcgttaggat atcggcggca aaatgcggta
      721 ttgacgcccc aaccctcaag ttctgcaagt aaggtatttc cacaactgtc atcgccgacg
      781 cgaccgatga aatcaacctc cacgccaagc tttgcgattg caacagcggc tgttgctgcc
      841 gggccgccac caatctcaag ataatgattt gcctgatatt tcccgccgcc ctccggcaga
      901 gtgggcaaag aataaattct gtcctgaact gtgataccca cgcatgcaat tctggtcata
      961 tttgtcttgt cctgtttaat tttgtcgtct ttggtcaatt taaatgacgc aaatgatcaa
     1021 attagtgatg cgtgtcgtgt ttttgaacat aaattacaaa tatgctcaaa aacagacgaa
     1081 aacgaatttt caattgtcat tattttgtca atgtcagaca ggagagtatt catggcaacc
     1141 attgcatttc tcgggctggg gcagatgggt agcccaatgg ctaacaatct attgcaaaaa
     1201 gggcattcat tgcaggtatt tgatgtcaat gcacaggccg tcgatgcgct agttacgcag
     1261 ggggccactg cggcacaaac cccagcggaa gcagcagcag gcgctgaatt tatcatcact
     1321 atgcttccca atggtgacat tgttcgacag gttctgttgg gggaaaaagg cgtctgtgag
     1381 gctgtttcat ctgacgcgtt attgattgat atgtcgacta ttcatccctt acagaccgat
     1441 gcattaatac gggagttaca agaaaaaggc atcagtatga tggacgctcc cgtgggcaga
     1501 acgtccgtga atgcgattga tggcacattg ctgattttag cgggtggcac tgatgagcag
     1561 attgcacgtg cacgcccgat tctcatgtgc atgggtaatg agctggtcga ggctggcggc
     1621 ccaggaatgg ggatccgcgt gaagctaatt aacaactata tgagtattgc gcttaatgcg
     1681 ctgtcctccg aggccgctgt gttatgtgag tcactgggat taaatcttga tgtcgcaatt
     1741 aaggtgatga gtggtacggc agcagggaaa ggacatttta cgaccacctg gcctggcaaa
     1801 gtgctgaaag gcgatctttc acccgccttc atggtggatc tggcgttgaa agatctgcgt
     1861 attgcagttg acgtcgctcg ggagacgggg gcaccgctca atatggggat tgcagcagaa
     1921 agttattacg ctgcagcaag tcaagacggc aaagggcggc aggactggag tgccctgcta
     1981 aatcaggttc gccagcaggc tggtctttaa ttttatctat taacaggagt ttctatgtca
     2041 tcccacttca ccccatttac gctgaaagat atctcccgcc caggtggtgg atttgccatg
     2101 ttagctgtcg atcagcgtga ggccatgcgc ttgatgtttg cggcagcagg gcagccaaaa
     2161 cccatcgctg acagtgtact caccgatttt aaagttgccg cgacccgaat cctttctcct
     2221 tatgcttctg ccgttctggc ggataaacaa ttttgtcttg agcaaatagt cgagcagggc
     2281 gctgttgcta attcttgcgg cctgattgtc gctgccgatc tttttatccc aggtaatggc
     2341 atcccggttg atagcgttga aatagatatg tccgtcgacc cgcataaagc ccgtgaaatg
     2401 ggggctaagg caatgaaatt acttgtactc tggcgtgaag atgaacccgc agaggaacgt
     2461 ctggcaatgg ttgataaatt tgttcgtcgc tgccgtagtg ccgggctggt gagcattatt
     2521 gaacctgtgg tgcgtccacc tcgtcgcggt tgggactttg atcgtgaaag caccattgtt
     2581 gccgccgccg cagagcttgg tggaacagag gctgatcttt acaaagccga gatgccatta
     2641 gggggcaaag gcgatgaaaa aaccttactt gccgcctgtc agcaacttaa cgatcagatg
     2701 aagatgccgt gggtaattct ttcttctggc gttgatgccg atatttttgg ccgtgcagta
     2761 agtattgcca tgaaaggtgg tgcgtcgggc ttcctggctg gacgagcggt ttgggcgtcg
     2821 gttgttggtg cacaagatcc acaaactatg ctgcgcgatg tctctgttcc tcgtttacaa
     2881 cggttggcag aaattgtcga tgaaggtatc gcgcaacgct aatcccctat ggatatcgat
     2941 tctggagaat aacaatggat aaaattaccc aggttttatt ttcagatatt gggaaagtca
     3001 ccactcaata tgttgaagta ccacaccagg aacttaaacc gcacgaagtg cggattgcgc
     3061 ctgtgttcta tgggatatgc ggttcggatc tgcatgttct gaaaggcggt catccgtttg
     3121 ccaaaccacc tgtcgtcccc ggtcatgaaa ttgcagcgcg cgttacggaa gttggcagcg
     3181 acgttaaaaa tgtacagccg ggcgatcatg ttgtggtcga tcccatcatg gcttgcatgg
     3241 aatgccgagc ctgcaaagca ggacgtttta atctttgtga accaccgcag gttgctggtt
     3301 ttcgcgcacc gggctttgct cgctcacaac acattgttcc tgcgcgtaat tgccatgtcg
     3361 caccagcctc tttaccgcta aaaatgttgg cctttgccga accggcggct tgtgcccgtc
     3421 actgcgttaa ccgaatgccg aaagcttctc tggaaagcgt actggtaatt ggtgccggaa
     3481 cgataggctt atccatcgtg caggcactgc gcattatggg ggcaggtaag attaccgtga
     3541 ttgaacctga cgctgccaaa cgcgcgctgg cgttaaagct gggcgcagca gaagtttggg
     3601 caccaggtga gctggccgca gatgtgcgat ttacgggggc gattgatgtc gttgcagcgc
     3661 aggccacgct taacgatgca tgtacccgtg tatatgccgg aggcaccgtc gtgtgcatgg
     3721 gcgtaccaag tgggccgcgt gaaataccat taccgatgat gcaacgtttc gagcgtgact
     3781 tgctcaactc tggcatgtac atccctgaag atttcgatgc tgttatcgaa tggctggcgg
     3841 atgggcggtt tgataccagt gaactggtta ccgatttatt tgccattgag gatgcagcgg
     3901 cggcatttga acgcgcgcag caaaatgact ccataaaggt catgctgcaa tttgcgccgg
     3961 aatgattttc taaatctctg tctggcgctg cgtcacgtca gcgccaccct acaacaatta
     4021 aaaaggacac tgcgatgagt caaatcacca gtcctgcaac ttattcaatt agtcggccac
     4081 aggacgtaat tgatattgtt aataagaact ctgcaatcaa caccagtatc ggcgttattt
     4141 ttattgcttt gggcggcata ttgattgatg cctaccaggc ggcgatggtg gggtttggta
     4201 ataaatacat tgccgctcaa ttcggcattt cgccgggtct tgctgcaacc gtcaatgcat
     4261 cagtattaat cgccgcgtta attggcggtt tattagcgaa ccgggtaata aaccgctttg
     4321 ggcaaaagcg ggcatttatt attggcatgg ggctgtgcac catcggtgct gcagcggtag
     4381 ctattgctcc cagtatctgg tgggtgctgg tgtgccgcgt catcatgggc tttggtttag
     4441 gcatcgactt ccctttggca accaatgccg tggcagagct tcgtggttca acgtcgaaga
     4501 aaaccggaac gtcggtcaac ctctggcaaa tggcctggta tgtttcgaca accgttgttt
     4561 atttggtact tttgccgctg cttctgtcgg gtatcgctga agaacaattg tggcgttacg
     4621 gaatattcat cggagctatt tttgcagtca tcttcatgat tttgcgttac ttctttattg
     4681 gtgaatccgc aatgtgggcc gcacgcgtcg ggcgttacca ggaagcgtgc gacattctgg
     4741 gaaaacgtta tggtgttcag gctcgcgttg cggcatcggg tacaacagaa gcgaaattct
     4801 cggaaaaagc agagaataaa tacagtggtg gatatggcat cttatttaat gatcgttacc
     4861 gcaaacgcac cattcttggc tgtgtcgtgg caaccatgca ggcgtggcaa tataacgccg
     4921 taggtgttta tcttcctctt acgttggcgg gaataataag tggcgggctt actggcgcgt
     4981 tgaccggttc tgccgtcgtg aatgcccttt gtggggtgac aggcgggatg atcggctcgt
     5041 ttattctcca acgactgggt actcgccgac agtcgatgta tggatttgct gttgtgacct
     5101 tagcattgct gtcgttaggc gcactggcaa cgactaatcc ctggctgtct ttagggttat
     5161 tgggatcaat tattttcttc cattcagcgg gtcctggtgg gctgggcatg accattgcca
     5221 cactctctta tcctcccgct attcgcccta ctggggtcgg atttgcccgc gctattatgc
     5281 gcacaggggc aattgcagga ctcattttct ggccgatgct gtggggtgcg ttgaaaactg
     5341 aagcgtttta ctggttggca atcgtgccat tcctggggtt cctgacctgc gtattgatta
     5401 attgggaacc actgggtgca aatgttgatg ctgaggatgc agaggttctg gctgaattga
     5461 agaaataaat taatgcgcat cggcttaaac cgatgcgcat taattcaaac tgcccacgtt
     5521 tagtggttcg tatcccagat ttctcgccta ccaggagagg aaaattatgt ctgaaaagtt
     5581 acccgcaccg cgtgaagggc tttccggtaa agccatgaga cgtgtcgtta tgggcagttt
     5641 cgcaggcgcg ttaatggaat ggtatgactt ctttattttt ggcacggcag caggactggt
     5701 atttgcaccg ctgttttatc cagacagcga tccttttata ggtcttatag ccgcctttgc
     5761 gacctttggt gtcggttttc tgactcgtcc tttaggtggc atcgtctttg gtcattttgg
     5821 cgataaaatc gggcgaaaaa taacacttat ctggacgctg gctattgtcg gttgctccac
     5881 atttttgatt ggttttattc cgacgtatca ggaaattggc atttgggcac cgcttgtatt
     5941 aatggtgctg cgtttgattc agggctttgg tcttggtggc gagtacggcg gagcggcatt
     6001 gatgaccata gaatcagcgc ccgaatcccg acgcggattt ttaggttcac tcccgcaaac
     6061 ggctgcgtct gtgggtatta tgctggcaac cggtattttt gctctctgta accattttct
     6121 tacttcggag cagtttctct cctggggatg gcgcattccg ttttggcttt ctgcggtgat
     6181 gttgatcgtt gggctgttta tccgcctgca taccgaagaa acactggatt ttcaaaagca
     6241 aaaaacgacg aataataaag aaaagtccgt tcctccgttg attgaattat tcaaaaaaca
     6301 tccacgaaat attttattgg cactgggtgc gaggctggcg gaaagtgtct cctctaatat
     6361 tattaacgcc tttggtattg tctatatttc cagccaatta gcattgtcgc gagatattcc
     6421 cctgacgggt atgttgattg cctcggcgat cggtattttc agttgtccat tggttggatg
     6481 gttatcagat cgtataggtc aaaaaagtct gtatttgtcg ggagcaggat tttgtgtcct
     6541 gttcgcgttt cctttcttct tattactgga tagcaaaagt acgctcatca tctggtgcag
     6601 tatgatcctc ggctacaact taggtccgac gatgatgttt gccgtgcagc caacactttt
     6661 cacccgcatg ttcggtacca aagtacgata taccggcctt tcttttgctt atcaattttc
     6721 cgcgattctt ggcggactaa gcccccttat cgcctcaagt ttgctggcgc tagggggagg
     6781 aaaaccgtgg tatgtcgctc tgtttttatt tgctgtctca gttctttcct ttgtttgcgt
     6841 ctggctgatt gagccgaccg atgaacaaga aaccgcttct taccgctaca tcagggaaca
     6901 atctcatgaa aactgaacga aaaaacgagc attacctggc gctacaacag gcgtttgacg
     6961 cgccatggcc tgggcctgta ggagagttag tgacgctgga aaaaggcaat attcacctgc
     7021 agatttatcc gcatgatggt gccaggataa cctcgttaaa ggcatttggt tctgaagtat
     7081 tacgccaatg gcagccgcag cgcagagctt ttcagtatgg ctgtttccca atggtgccgt
     7141 gggcagggcg actggggaat gcgacgctga atgctggtgg gcaatgttat tctttacccg
     7201 ccaataaacc accacatgcg ttgcatggta tggcttgtta ctcaacgtgg gaaatcatcg
     7261 acaaaacagc agattccctg actttacgta tgcccctggc ttcaccgtgg ccctggcaag
     7321 gggaagtgat tcagactttt ttgctggaga acgatgcgct ggttttgcaa ctggaagtcc
     7381 attcatatgc cgatacattc ccggcctccg caggctggca tccgtggttt gcgaaaaagc
     7441 tgactccgca aaatacagaa tcgttgcagg tgctttttga tgcggactgg caagaagaag
     7501 cgggtagtga tgaactgcca actggaaatc gtatctttcc tcaagccggt ccctgggatg
     7561 actgttttgg tttttataac ggggtgaagg ttaagttact gtggcctggt aaactcgcga
     7621 tgaccatgat ctcttctgca aacagtctgg tggtatttga taaacagccc gatgccacct
     7681 gtattaaccc gctgactcag gcacctaacg caataaatct aacgccggaa ttggtgacac
     7741 cagataggcc gctggtcatc gaaactcgtt ggcaatttac acctgaatct taaccattca
     7801 tctattgcga aaaagccagt gaaattactc tgctttttcg caatcagcat cacaagatta
     7861 cccttattgc cacatagcca aacccatcct ttcccgctac agttaatttc ttgtggcgcg
     7921 aaaggaggca aaaatgctct atatctttga tttaggtaat gtgattgtcg atatcgactt
     7981 taaccgtgtg ctgggagcct ggagcgattt aacgcgtatt ccgctggcat cgcttaagaa
     8041 gagttttcat atgggggagg cgtttcatca gcatgagcgt ggggaaatta gcgacgaagc
     8101 gttcgcagag gcgctgtgtc atgagatggc tctaccgcta agctacgagc agttctctca
     8161 cggctggcag gcggtgtttg ttgcgctgcg cccggaagtg atcgccatca tgcataaact
     8221 gcgtgagcag gggcatcgcg tggtggtgct ttccaatacc aaccgcctgc ataccacctt
     8281 ctggccggaa gaatacccgg aaattcgtga tgctgctgac catatctatc tgtcgcaaga
     8341 tctggggatg cgcaaacctg aagcacgaat ttaccagcat gttttgcagg cggaaggttt
     8401 ttcacccagc gatacggtct ttttcgacga taacgccgat aatatagaag gagccaatca
     8461 gctgggcatt accagcattc tggtgaaaga taaaaccacc atcccggact atttcgcgaa
     8521 ggtgttatgc taaaaaccat tcaggacaaa gccaggcacc gtacccgtcc actatgggcc
     8581 tggctaaaac tactctggca acgtattgat gaggacaaca tgacaaccct ggcaggtaac
     8641 cttgcctatg tgtctttgct ctcattagtg ccgctggttg ccgttgtttt tgcgcttttc
     8701 gccgcttttc ccatgttttc cgacgtcagc attcagttac gtcactttat ttttgccaac
     8761 tttctgcctg ctactggcga tgttattcag cggtatatcg aacaatttgt tgccaattcc
     8821 aacaagatga ccgccgttgg ggcgtgcggg ctgatcgtca ccgcgttatt gttgatgtac
     8881 tccatcgata gcgcgttgaa taccatctgg cgcagtaaac gagcgcgacc aaaaatttac
     8941 tcgtttgccg tgtactggat gatcttaacg ctggggccgc tgctggcagg ggccagtctg
     9001 gcgatcagtt cctatttgtt ctccctgcgc tgggcgagcg atctcaatac tgtcatcgat
     9061 aacgtgctgc gtatttttcc gctgctgttg tcgtggatct ccttctggtt gctgtacagc
     9121 attgttccta ccatccgcgt acctaaccgc gacgcgattg tcggcgcgtt tgtcgctgca
     9181 ctcctgttcg aagcaggaaa gaaaggtttc gcgctttata tcaccatgtt cccgtcatat
     9241 cagctcattt acggcgtgct ggcggtgatc cccattctct tcgtttgggt ctactggaca
     9301 tggtgtatcg tcttgcttgg cgcggaaatt actgtcactc tcggggaata ccgcaaacta
     9361 aaacaagcag ctgaacaaga agaagacgac gaaccatgat tgcattaatt caacgcgtaa
     9421 cccgtgccag cgtcaccgtg gagggagaag tgacgggcga aattggcgcg ggacttttgg
     9481 tgttattggg tgtcgaaaag gatgacgacg aacagaaagc aaaccgtctg tgcgagcgtg
     9541 tgctcggcta ccgcatcttt agcgatgccg aaggcaagat gaatctcaac gtgcaacagg
     9601 cgggcggcag tgtactggtg gtttcccagt ttaccctcgc cgcagatacc gaacggggga
     9661 tgcgcccaag tttctccaaa ggcgcatcac cggatcgcgc agaggcgtta tatgactatt
     9721 tcgtcgaacg ctgccgccag caagagatga acacgcaaac aggacgcttc gctgccgata
     9781 tgcaggtatc gctggtcaat gatggccccg tgacattctg gttgcaggta tgagcctgct
     9841 tccagggttg tcacgggaaa caagagagag tatcgctatg tatcaccttc gggttccaca
     9901 aacagaagaa gagttagagc gttactatca gtttcgctgg gaaatgttgc gtaagcccct
     9961 gcatcaacca aaaggttcgg aacgcgacgc gtgggatgcg atggcgcatc accagatggt
    10021 cgtcgacgag cagggtaatc tggtggcggt aggccgattg tatattaatg ccgacaatga
    10081 agcgtccatt cgctttatgg ccgttcaccc ggacgtgcag gacaaagggt taggaacgct
    10141 gatggcgatg accctggagt cggtggcgcg tcaggaaggc gttaagcgcg tgacctgtag
    10201 cgcccgtgaa gacgcggtgg agtttttcgc caagctggga ttcattaatc agggggagat
    10261 caccacccca actaccacgc cgattcgcca ttttttgatg attaaacccg ttgccactct
    10321 ggatgacatt ctgcatcgcg gtgactggtg cgcgcagctg caacaggcgt ggtacgaaca
    10381 tatcccgtta agcgaaaaaa tgggcgtgcg cattcagcaa tataccgggc aaaaatttat
    10441 caccaccatg ccagaaaccg gtaatcagaa tccgcaccat acgttgtttg ccgggagttt
    10501 attctcgctg gcgacgctca ccggttgggg acttatctgg ctgatgctgc gtgaacgcca
    10561 cctcggcgga acgattattc tggcggatgc gcatatccgc tacagcaagc cgattagcgg
    10621 taaacctcat gcggtagccg accttggtgc cttaagcggc gatctcgacc gtctggcgcg
    10681 cggacgaaaa gcacgggtgc agatgcaagt tgaaatcttt ggcgacgaga cgccgggtgc
    10741 agtgtttgaa ggcacgtata tcgttctgcc cgcgaagcca tttggcccgt atgaagaggg
    10801 cgggaacgaa gaagagtagc ttatggagag gggcggtgct gcctctctca ttcattccgg
    10861 tc
//
LOCUS       DAETQN010000140        10844 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.141, whole genome shotgun sequence.
ACCESSION   DAETQN010000140 DAETQN010000000
VERSION     DAETQN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.141"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="K0W68_003662"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="K0W68_003662"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBI3075116.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="K0W68_003663"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="K0W68_003663"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBI3075117.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="K0W68_003664"
     CDS             complement(2698..3390)
                     /locus_tag="K0W68_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBI3075118.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /locus_tag="K0W68_003665"
     CDS             3520..5280
                     /locus_tag="K0W68_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HBI3075119.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5686..6543
                     /gene="focA"
                     /locus_tag="K0W68_003666"
     CDS             5686..6543
                     /gene="focA"
                     /locus_tag="K0W68_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBI3075120.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6598..8880
                     /gene="pflB"
                     /locus_tag="K0W68_003667"
     CDS             6598..8880
                     /gene="pflB"
                     /locus_tag="K0W68_003667"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBI3075121.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9072..9812
                     /gene="pflA"
                     /locus_tag="K0W68_003668"
     CDS             9072..9812
                     /gene="pflA"
                     /locus_tag="K0W68_003668"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBI3075122.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9905..10081)
                     /locus_tag="K0W68_003669"
     CDS             complement(9905..10081)
                     /locus_tag="K0W68_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005043118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBI3075123.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNIISRYYLRYGIW"
     gene            10181..>10844
                     /locus_tag="K0W68_003670"
     CDS             10181..>10844
                     /locus_tag="K0W68_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3075124.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     ILD"
BASE COUNT         2792 a         2712 c         2569 g         2771 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tttttaatgc cccgctttac atatatttgc attaataaaa taattgtaat tataaggtta
     5401 aatatcggta atttgtattt aataaatacg atcgatattg ttactttatt cgcctgatgc
     5461 tcccttttaa ttaactgttt tagcggagga tgcggaaaaa attcaactca tttgttaatt
     5521 tttaaaattt atttttattt ggataatcaa atgtttactc cgtatctgca taaaaaccat
     5581 gcgagttacg ggcctataag ccaggcgaga tatgatctat atcaatttct catctataat
     5641 gctttgttag tatctcgtcg ccgacttaat aaagagagag ttagtgtgaa agctgacaac
     5701 ccttttgatc ttttacttcc tgctgcaatg gccaaagtgg ccgaagaggc gggtgtctat
     5761 aaagcaacga aacatccgct taagactttc tatctggcga ttaccgccgg tgttttcatc
     5821 tcaatcgcat tcgtcttcta tatcacagca accactggca caggcacaat gcccttcggc
     5881 atggcaaaac tggttggcgg catttgcttc tctctggggc tgattctttg tgttgtctgc
     5941 ggagccgatc tctttacttc caccgtgttg attgttgttg ctaaggcgag tgggcgcatc
     6001 acctggggtc agttggcgaa aaactggcta aatgtctatt ttggcaacct ggtcggcgca
     6061 ctgctgtttg tacttttaat gtggctttcc ggcgagtata tgaccgcaaa tggtcaatgg
     6121 ggactaaacg tcctacaaac cgccgaccac aaagtgcacc atacttttat tgaggccgtc
     6181 tgtcttggta tcctggcaaa cctgatggta tgtctggcag tatggatgag ttattctggc
     6241 cgcagcctga tggacaaagc gttcattatg gtgctgccgg tcgcgatgtt tgttgccagc
     6301 ggttttgagc acagtatcgc aaacatgttt atgatcccga tgggtattgt aatccgcgac
     6361 ttcgcatccc cggaattttg gaccgcagtc ggttctgcac cggaaaattt ttctcacctg
     6421 accgtgatga atttcatcac tgataacctg attccggtta cgatcggcaa cattatcggt
     6481 ggtggtttgt tggttgggtt gacatactgg gtcatttacc tgcgtgaaaa cgaccaccat
     6541 taatggttgt cgaagtacgc agtaaataaa aaatccactt aagaaggtag gtgttacatg
     6601 tccgagctta atgaaaagtt agccacagcc tgggaaggtt ttaccaaagg tgactggcag
     6661 aatgaagtaa acgtccgtga cttcattcag aaaaactaca ctccgtacga gggtgacgag
     6721 tccttcctgg ctggcgctac tgaagcgacc accaccctgt gggacaaagt aatggaaggc
     6781 gttaaactgg aaaaccgcac tcacgcgcca gttgactttg acaccgctgt tgcttccacc
     6841 atcacctctc acgacgctgg ctacatcaac aagcagcttg agaaaatcgt tggtctgcag
     6901 actgaagctc cgctgaaacg tgctcttatc ccgttcggtg gtatcaaaat gatcgaaggt
     6961 tcctgcaaag cgtacaaccg cgaactggat ccgatgatca aaaaaatctt cactgaatac
     7021 cgtaaaactc acaaccaggg cgtgttcgac gtttacactc cggacatcct gcgttgccgt
     7081 aaatctggtg ttctgaccgg tctgccagat gcatatggcc gtggccgtat catcggtgac
     7141 taccgtcgcg ttgcgctgta cggtatcgac tacctgatga aagacaaact ggcacagttc
     7201 acttctctgc aggctgatct ggaaaacggc gtaaacttgg aacagactat ccgtctgcgc
     7261 gaagaaatcg ctgaacagca ccgcgctctg ggtcagatga aagaaatggc tgcgaaatac
     7321 ggctacgaca tctctggtcc ggctaccaac gctcaggaag ctatccagtg gacttacttc
     7381 ggctacctgg ctgctgttaa gtctcagaac ggtgctgcaa tgtccttcgg tcgtacctcc
     7441 accttcctgg atgtgtacat cgaacgtgac ctgaaagctg gcaagatcac cgaacaagaa
     7501 gcgcaggaaa tggttgacca cctggtcatg aaactgcgta tggttcgctt cctgcgtact
     7561 ccggaatacg atgaactgtt ctctggcgac ccgatctggg caaccgaatc tatcggtggt
     7621 atgggcctcg acggtcgtac cctggttacc aaaaacagct tccgtttcct gaacaccctg
     7681 tacaccatgg gtccgtctcc ggaaccgaac atgaccattc tgtggtctga aaaactgccg
     7741 ctgaacttca agaaattcgc cgctaaagtg tccatcgaca cctcttctct gcagtatgag
     7801 aacgatgacc tgatgcgtcc ggacttcaac aacgatgact acgctattgc ttgctgcgta
     7861 agcccgatga tcgttggtaa acaaatgcag ttcttcggtg cgcgtgcaaa cctggcgaaa
     7921 accatgctgt acgcaatcaa cggcggcgtt gacgaaaaac tgaaaatgca ggttggtccg
     7981 aagtctgaac cgatcaaagg cgatgtcctg aactatgatg aagtgatgga gcgcatggat
     8041 cacttcatgg actggctggc taaacagtac atcactgcac taaacatcat ccactacatg
     8101 cacgacaagt acagctacga agcctctctg atggcgctgc acgaccgtga cgttatccgc
     8161 accatggcgt gtggtatcgc tggtctgtcc gttgctgctg actccctgtc tgcaatcaaa
     8221 tatgcgaaag ttaaaccgat tcgtgacgaa gacggtctgg ctatcgactt cgaaatcgaa
     8281 ggcgaatacc cgcagtttgg taacaacgat ccgcgtgtag atgacctggc tgttgacctg
     8341 gtagaacgtt tcatgaagaa aattcagaaa ctgcacacct accgtgacgc tatcccgact
     8401 cagtctgttc tgaccatcac ttctaacgtt gtgtatggta agaaaacggg taacacccca
     8461 gacggtcgtc gtgctggcgc gccgttcgga ccgggtgcta acccgatgca cggtcgtgac
     8521 cagaaaggtg cagtagcctc tctgacttcc gttgctaaac tgccgtttgc ttacgctaaa
     8581 gatggtatct cctacacctt ctctatcgtt ccgaacgcac tgggtaaaga cgacgaagtt
     8641 cgtaagacca acctggctgg tctgatggat ggttacttcc accacgaagc atccatcgaa
     8701 ggtggtcagc acctgaacgt taacgtgatg aaccgtgaaa tgctgctcga cgcgatggaa
     8761 aacccggaaa aatatccgca gctgaccatc cgtgtatctg gctacgcagt acgtttcaac
     8821 tcgctgacta aagaacagca gcaggacgtt attactcgta ccttcactca atctatgtaa
     8881 ttagatttga ctgaaatcgt acagtaaaaa gcgtacaata aaggctccac gaaagtgggg
     8941 ccttttttag cacgagagcc ttttttgtca gctatctata ctctaaggtg actgccaaaa
     9001 cagactcgac gtagccttcg agctgcgcac caacacggcc tcagatgggc cacatctgga
     9061 gaaacaccgc aatgtcagtt attggtcgca ttcactcctt tgaatcctgt ggaaccgtag
     9121 acggcccggg tattcgcttt atcacctttt tccagggctg cctgatgcgc tgcctgtatt
     9181 gtcataaccg cgacacctgg gatacgcatg gcggtaaaga agttaccgtt gaagatttaa
     9241 tgaaagaggt ggtgacctat cgccacttta tgaacgcttc cggcggcggc gttaccgcat
     9301 ccggcggtga ggcaatccta caagctgagt ttgttcgtga ctggttccgc gcctgcaaaa
     9361 aagaaggcat tcatacctgt ctggacacca acggttttgt tcgtcgttac gatccggtga
     9421 ttgatgaact gctggaagta accgacctgg taatgctcga tctcaaacag atgaacgacg
     9481 agatccacca aaatctggtt ggagtttcca atcaccgcac gctggagttc gccaaatatc
     9541 tggcgaacaa aaatgtgaag gtgtggatcc gctacgttgt tgtcccaggc tggtctgacg
     9601 atgacgattc agcgcatcgc ctcggtgaat ttacccgtga tatgggcaac gttgagaaaa
     9661 tcgagcttct cccctaccac gagctgggca aacacaaatg ggtggcaatg ggtgaagagt
     9721 acaaactcga cggtgttaaa ccaccgaaga aagagaccat ggaacgcgtg aaaggcattc
     9781 ttgagcagta cggtcataag gtaatgttct aatttccccg taaagcggca actttattga
     9841 gttgccgctt ttttattccg cacttcattc tgctacagcg tttttaacac atcttatagt
     9901 gcatctacca tatcccgtac ctgagataat aacgactgat aatattacgc cgatataaat
     9961 cgagttccgt cacctgtatt ttccgctcca ttgcccgctg atggatcgca tcagcaatat
    10021 gtgcggtcaa tgaatcatga cgaggatggg cccataccaa ataaatacgt tcagactgca
    10081 taattttacc tccacgttaa gttctggagc gcactttacg caggcggcat aataatgtaa
    10141 acgtgatgat tcaggatgag ttaatgaagg attttcacta atgcggatga atatgtctga
    10201 ctttgccact ttctttgctg tggcccgtaa tcaaagcttt cgtgcagcgg gcgatgagtt
    10261 aggcttatcc tcgtccgcca ttagccatag tattaaaaca ctggaacaac gtcttaaaat
    10321 tcgcttattc aatcggacaa cccgaagcgt atcgttaacg gaagcgggtt ctaatctcta
    10381 tgaacgattg cgcccagctt tcgacgaaat tcagatcatg ttagatgaga tgaatgattt
    10441 tcgtctgaca ccaacaggaa cgctcaaaat caatgccgcc cgggtagctg cacgtatatt
    10501 tttaatgcct ttactggttg gtttcacacg tgaatatcct gatattaaag tcgagctcac
    10561 aactgacgac tctcttgtcg atatcgtcca acagggcttt gacgctggcg ttcgacttag
    10621 tggcatcgtc gaaaaagata tgatcagcgt ggcgatcggc ccaccagtga aattatgtgt
    10681 agcagcaacg ccagagtact ttgcaagata tggcaaaccc cgccatcctc atgatctgct
    10741 taatcatcaa tgtgttgtat ttcgttatcc cagcggcaaa cctttccact ggcaatttgc
    10801 aaaggagcag gaaattgctg tcgcagggaa tatcattctg gatg
//
LOCUS       DAETQN010000141        10717 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.142, whole genome shotgun sequence.
ACCESSION   DAETQN010000141 DAETQN010000000
VERSION     DAETQN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10717
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.142"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..1092
                     /gene="dacC"
                     /locus_tag="K0W68_003671"
                     /pseudo
     CDS             1..1092
                     /gene="dacC"
                     /locus_tag="K0W68_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="K0W68_003672"
     CDS             complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="K0W68_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBI3075125.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="K0W68_003673"
     CDS             complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="K0W68_003673"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBI3075126.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2836..4063
                     /gene="mdfA"
                     /locus_tag="K0W68_003674"
                     /pseudo
     CDS             2836..4063
                     /gene="mdfA"
                     /locus_tag="K0W68_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4104..4388)
                     /locus_tag="K0W68_003675"
     CDS             complement(4104..4388)
                     /locus_tag="K0W68_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075127.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="K0W68_003676"
     CDS             complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="K0W68_003676"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBI3075128.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5289..6497)
                     /locus_tag="K0W68_003677"
     CDS             complement(5289..6497)
                     /locus_tag="K0W68_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3075129.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6581..7117
                     /gene="rcdA"
                     /locus_tag="K0W68_003678"
     CDS             6581..7117
                     /gene="rcdA"
                     /locus_tag="K0W68_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBI3075130.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7292..8977)
                     /locus_tag="K0W68_003679"
     CDS             complement(7292..8977)
                     /locus_tag="K0W68_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBI3075131.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9247..9624
                     /locus_tag="K0W68_003680"
     CDS             9247..9624
                     /locus_tag="K0W68_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBI3075132.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="K0W68_003681"
     CDS             complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="K0W68_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBI3075133.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10071..10358
                     /locus_tag="K0W68_003682"
     CDS             10071..10358
                     /locus_tag="K0W68_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBI3075134.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10342..>10717
                     /gene="nfsA"
                     /locus_tag="K0W68_003683"
     CDS             10342..>10717
                     /gene="nfsA"
                     /locus_tag="K0W68_003683"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBI3075135.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLG"
BASE COUNT         2765 a         2650 c         2714 g         2588 t
ORIGIN      
        1 atgcgcgtgc atggatttta atggattacg ccagcggtaa agtgctggca gaaggcaacg
       61 cggatgagaa actggatccc gcgagcctga ctaaaatcat gaccagctat gtggttgggc
      121 aggcgcttaa ggccgataag attaaactca ccgatatggt gacggtcggt aaagatgcct
      181 gggcgacggg aaatccggca ctgcgtggtt catcggtaat gttcctcaaa ccgggcgatc
      241 aggtttcggt ggcagacttg aacaaaggtg tgattatcca gtccggtaat gacgcctgta
      301 ttgcgctggc cgattacgtt gccgggagcc aggagtcatt tattggtttg atgaatggtt
      361 atgccaaaaa aactgggtct gaccaacact accttccaga cggtgcacgg tctggatgcg
      421 ccggggcagt tcagtaccgc gcgcgatatg gcattgctgg gtaaagcatt gatccacgat
      481 gtgccggaag agtacgccat tcataaagag aaagagttca ccttcaacaa aattcgtcag
      541 cctaaccgta accgtctgct gtggagcagc aatctgaatg ttgatggcat gaagacagga
      601 accacggcag gtgcgggata taatctggtt gcttcggcta cccagggtga tatgcgttta
      661 atctccgtag tgctgggggc gaaaactgac cgtatccgtt ttaatgagtc tgagaaatta
      721 ttgacctggg gtttccgctt ctttgaaact gtgacgccaa ttaaacctga tgccaccttt
      781 gtgactcagc gcgtctggtt tggtgataag agcgaagtga atctcggggc aggcgaagcg
      841 ggctccgtga ccataccgcg tgggcagctg aaaaacctga aggcgagcta cacgttaacg
      901 gaaccgcagc ttaccgcacc gctgaaaaaa ggtcaggttg tcgggaccat tgatttccag
      961 cttaacggta aatccattga gcagcgtccg ctgatcgtga tggaaaatgt ggaagagggc
     1021 ggattctttg gtcggatgtg ggatttcgtg atgatgaaat tccatcagtg gttcggcagc
     1081 tggttctctt aatcttctga taaccggatg gcgcgaaacg tcatccggtt atacgtcatt
     1141 aatacatcaa cttaatacgg tgcgtctgcg cgtacttcac atactcatct tccgggcaac
     1201 aatcactcac cacaatatca aagcgtttca ggtcacccat gcgcgccgga cgcaccttgc
     1261 caaatttact gtggtcgaca accagcacat gcttttgcgc catcgacatg gcccagtgtt
     1321 ttaccggcaa ctcttcaaga ttaaaacagg tagcgccttt actgacatgc acgcccgccg
     1381 cggaataaaa agcgatatcc gggcaaaaat tattcaacgt ttgctgaaaa tcgatggggt
     1441 tgaaaatggc gttgctggcg tgaaattcac caccgcacag aaatgcgcgg caatggggtt
     1501 tctctttcag cgccagaaag gtatttagcg aataacaaac ggcggtaaaa gggatttcat
     1561 tatcaatcgc ttcaataatc cacggcgtgg tggtgccaca gtcaaaaaag agggtctgat
     1621 cgggttctac cagtgtcgca gccagttttg ccgcccggcg tttttcttcc accaggcggg
     1681 atttttgatc gcttaacagg taatggctgg cactgcgcgg ttccagaaca atatagccgc
     1741 cgagcaaaac gacgggcgca ctgtggttgt tcagatcgcg acgaatcgtc atctccgaaa
     1801 ccccaagcag ggcggcggcg tctttaagat gtaacttatc gctgcgtttt aattcttgca
     1861 gcagctgccc gatacgctct tcgcgacgtg tttccataat ccctctgaat agttattgaa
     1921 gcgagccgct caatactaca ctttttagca gagatcagtc acgcacccag cctttgcgga
     1981 tcggtaatgc aaaacagacg cgataccacg attgcagacg ttgatagagt ttatgcccca
     2041 tcgcttgcca gataatctgg gcactaaggc agccaatcat acctgccagc aatccaccga
     2101 gcatatccag cggccagtgg acgccaagat aaacgcgcga ccaggcaatg acgacggcca
     2161 gaaccattaa aagtgagccg gaccacaggc gatgccagca taaaaatgcc agtgcaaagg
     2221 tgaaaatcac cgtaccgtga tcgctcggga atgagtcatc cgccgcatga tgcaggaagt
     2281 tatagccgat attttcgaca aagggtcggt cgtgcggaaa aagatgtccc atcgtccagg
     2341 acacaaacag gctgaccgcc agcgcgatag cgatttttat caccagttgc cgttgtgctg
     2401 taagccccca caaccaaagt accgcggcca gcaacggcac cacggtaatc aaatctttag
     2461 caataaaaat cgccaacgag atcatccacg gagccgagtc tggcgtcgcg ttaataagag
     2521 agaatagaga gagattcaaa ttttccagca taacttcccg acgcaaagtg attaaaaggg
     2581 agccaataca ggcaagtcgt tgagaataaa gtgcaggtta aactgggtaa agcggcatcg
     2641 tcttatttcc ctcaagcggc ctgtttacgg tgggtgattg taacgggcat aggttaaata
     2701 aaacttaaag aaagcgtagc tatactcgca ataatgtaag aatgtgctta accgtggttt
     2761 cagctacaaa attcgctttc tcgttagctg cgcttttatt aaactctgcg cgattattat
     2821 tggcgaagaa attgcatgca aaataaatta gctttcggtg ccaggcttgg acgtcaggcg
     2881 ttacttttcc ctctctgtct ggtgctttac gaattttcaa cctatatcgg caacgatatg
     2941 atccaacccg gtatgttggc cgtggtggaa caatatcagg cgggcattga ttgggttcct
     3001 acttcgatga ccgcgtatct ggcgggcggg atgtttttac aatggctcct ggggccgctg
     3061 tcggatcgta ttggtcgccg tccggtgatg ctggcgggtg tggtgtggtt tatcgtcacc
     3121 tgtctggcaa tattactggc gcaaaacatt gaacaattca ccctgttgcg cttcttgcag
     3181 ggcataagcc tctgtttcat tggcgctgtg ggatacgccg caattcagga atccttcgaa
     3241 gaggcggttt gtatcaagat caccgcgctg atggcgaacg tggcgctgat tgctccgcta
     3301 cttggtccgc tggtgggcgc ggcgtggatc catgtgctgc cctgggaggg gatgtttgtt
     3361 ttgtttgccg cattggcagc gatctccttt ttcggtctgc aacgagctat gcctgaaacc
     3421 gccacgcgta taggcgagaa actgtcactg aaagaactcg gtcgtgacta taagctggtg
     3481 ctgaagaacg gccgctttgt ggcgggggcg ctggcgctgg gattcgttag cctgccattg
     3541 ctggcgtgga tcgcccagtc gccgattatc atcattaccg gggagcagtt gagcagctat
     3601 gaatatggct tgctgcaagt gcctattttc ggggcgttaa ttgcgggtaa cttgctgtta
     3661 gcgcgtctga cctcgcgccg caccgtacgt gataattatg ggcggctggc cgattatgat
     3721 tggtctgttg gtcgctgctg cggcaacggt tatctcatcg cacgcgtatt tatggatgac
     3781 tgccgggtta agtatttatg ctttcggtat tggtctggcg aatgcgggac tggtgcgatt
     3841 aaccctgttt gccagcgata tgagtaaagg tacggtttct gcggcgatgg gaatgctgca
     3901 aatgctgatc tttaccgttg gtattgaaat cagcaaacat gcctggctga acgggggcaa
     3961 cggacagttt aatctcttca accttgtcaa cggaattttg tggctgtcgc tgatggttat
     4021 ctttttaaaa gataaacaga tgggaaattc tcacgaaggg taaaaaaatg cctgactgct
     4081 ttgtgcgatc aggcattctc gaattaatgg tgatggtcgt caatctggtg ttcgataacc
     4141 atcccttcac ctacgctggc aagatggcga gcataaggat gcgggcggta agccggagct
     4201 ggcgcaggag ccacatagac ggtttgcggc acagtcgcga cgctgaccgc ttgtggaacg
     4261 ctgactgaat caggaactac aaccactttg tatccactcg gcacatcgac ggtgacactt
     4321 tgcgccatcg ccacaccagt aaagcccatt aaaagcgcgc ccaacagtag acaatttttc
     4381 ataatcattt cgcctgaaat aatgcagcgg gttaatttcc gcgaattatg cagccttcat
     4441 tcaggtattg tgttgccggg cgtgctgccg tttttattgg tcaaatggcg cttcgtgttt
     4501 aagcacttta tcgatcacat ccagtacgcc ttcacggtta ttggagcctg cccggtattt
     4561 tgccgctgcg acgaccgcgc tgccggcatt ttccattgca aaactaaagc ctgcctgacg
     4621 cagcatctca atatcgttac cgccatcgcc aaagaccacc acttcgctgt cgtctattcc
     4681 ccataatttc tgcagttggc gaaggccatt ggctttatgt acgccgggga taatcagatc
     4741 gatgctgccg ttgccggtgt ggactggcac cataatatcg ccgatggcct catgtaatgc
     4801 tttttgtact tgtggaatca gttcatcgga aagattcagg ccaaacttaa agaaaatatc
     4861 ttctaagttg tcaaagttat cgacgtattc cagacggtga taatacattt ctgccaccgt
     4921 tttcatggca tcgtcatatt ttttgagcgt ataggcgcta ttttttccgc aggcaataat
     4981 ttccacttcc gggcgcgtca gcaaatgttc cacgacagta gtaaaggcat ccttcgacag
     5041 ctcgccatta aaaacatctt tgccttcact cactacccag ccgccgtttt cagccacaaa
     5101 ggcaatttca ttagcaattt cagggaagaa agagagcaac tgataatatt gattcccgct
     5161 ggcgaccaca aagcgaatgc cttgtgcttt catttgctga tactgagcca taaaccgctc
     5221 acggttatag gttttttgat cgcttaagaa agtaccatcc atgtctaccg caattaattt
     5281 aatgctcatc aactattctc catcgccgtc cgcgttttgg tatcgggttt ggcgacggct
     5341 ttcgcgacaa tagccgcgag aataaccagc gccagtacaa ccagcattgc actacgtaat
     5401 ccataatgtt cgccgagata gcccagcagc ggcggcccga cgaggaaagc cagataaccg
     5461 gtcgttgcta ccacactgac gcgggttggt gcatcggggc cggtatcgct ggcggcagaa
     5521 atggtcagcg ggaagcccag cgaggcaccc agtccccaga gtacaacaga caccccagcg
     5581 acccaggcgc tatcgacaaa aataatcagc ccaataccca acgcccccat tagtgcactg
     5641 gcccgaacca cggcaacgcg actgtaacgg tcgatgaacc aaccgccagt gaagcgtcca
     5701 acggtcatcc ctagggtaaa accggcataa atcagcgagc cggaagtagg gctaaaaccg
     5761 tgaccgtcaa ccattaataa gggtaaccag tcgttggcag aaccttcggc aaaggccatc
     5821 gccagcacta caacaccaat caacagcaac tggatatcgc gataaaaagg aatgcctttt
     5881 tcgccatgct gggtgccatc ggcagcattt ttgcccgtac cgtcagggat tgcctgaatg
     5941 gcgatataaa taggcgcgat acctaccagc gccgccaata aaatgtgcac cgttgccgga
     6001 acgccaaagg ccgtcagtgc catcccgaca ccagcgcctg ccagcgtgcc taggctataa
     6061 aaaccgtgca tcatcggcaa aaccgtttta ttcatttctc gctcaacggc ggcaccttca
     6121 acgtttatcg ccacttccgc agaaccaaaa cttgccccaa agacgccgag accaacggca
     6181 aagagcaggg gcgatgtcag ccagagtgcc agacttaata tcatcatccc gatcaatgcg
     6241 caggacatcg tgaccaggat gacattacgt gtcccaaagc gtttcactaa ccacgccgag
     6301 cagagaatac cgctcatcga accgatcgac agaccaaaga gaacaccgcc catttcagcg
     6361 atcgagacag agagaatatc gcggatagca ggcgtacggg ttgcccagga cgccattaac
     6421 aggcctggca aaaagaagaa cataaacagc gcccaggttc ggcgtttcaa tgcattacgt
     6481 gaagaattta cggtcataga tcacgtcaaa ataagaagag ggaagacaac attagcaagg
     6541 ttgtgtacat ttgtacacaa ttgcagacag aggaaatgac atgcgtcgcg ctaacgatcc
     6601 gcaacggcga gaaaaaatta tccaggccac gctggaggcg gtgaaacttt acggaataca
     6661 tgctgttacg caccgcaaaa ttgctaccct tgccggggta ccgttgggat cgatgaccta
     6721 ctatttttca ggaattgatg agttgttgct ggaggcgttc agcagtttta ctgagattat
     6781 gtcccggcaa tatcaggcat tttttagcga tgttagtgat gctcaaggcg catgccaggc
     6841 tatcaccgat atgatctaca gctcacaggt tgcaacgccg gataatatgg agctgatgta
     6901 ccagctctac gcgctggcta gccgaaaacc gctattaaaa acggtaatgc aaaactggat
     6961 gcagcgcagt cagcaaacgc tcgaacaatg gtttgaaccc ggaaccgccc gcgcgcttga
     7021 tgcgtttatt gaggggatga cgctgcattt tgtcaccgac cgtaagccgc tatcgcgcga
     7081 ggagattttg aggatggttg agagggttgc agggtagtag ataaatttta gataacaaaa
     7141 aacccatcaa ccttgaaccg aaatggcggg gttgatgggc tccacaaaat ggggacatca
     7201 aagaaaagca gtggcactaa ttaagactga tgccctgcgg aaaagttctg cggttgtgca
     7261 aaaaaatttc attttcaggg caacttcagt tttatcctaa tcctggccat accatgacga
     7321 tgattgtccc tgccagcgtc agcaggacgt tggcgattgc gtaggtgccc gcatagccca
     7381 gcgcagggat gttactgcga gctgtatcac tgatgatctc cattgccggc gcgcaggtgc
     7441 gtgcgcccat cattgcgccg aacaacagtg cgcggttcat tcgcaataca taagcaccga
     7501 acaagaaaca gataaccacg ggcacaagac tgacaattaa tccggcaatc aacatctgac
     7561 cgccaatcgc gcccaggccg ttattaatac cgctaccggc gctcagacca acgcctgcca
     7621 taaacaccat caagccgaac tctttcacca tgcttaatgc accttgcgga atgtaaccga
     7681 aggtcgggtg gttagcacgc ataaagccca gcataattcc ggcgaataac aacccggcag
     7741 cgttccccat gccgaaactg aatgtgctga actggaaggt gatcatcccg atcatcagcc
     7801 caataacaaa gaaggcgcaa aatgccagca ggtcagtgac ctggctgtga atcgagataa
     7861 agccgatgcg atcggcgatg gtttttacgc gacgggcatc accgctgact tgtaaaacgt
     7921 cacctttgtt aagcacgacg ttgtcatcta tcggcatctc aatctggcta cgaatgacgc
     7981 ggttaaggaa gcaaccgtga tcggtcaact tcagttgtgc gagacgttta cctacagcgt
     8041 tatggttttt aacgaccact tcttcagtga cgatacgcat gtcgagaagg tcacgatcga
     8101 aaacttcttt accgttacgg aagctgggat cgagtcgggc atgggcgtcg ggatagccta
     8161 ccaacgctat ttcatcgccc atttgtagca cggcatcacc gtctggattt gccagaatcc
     8221 cgttacgtcg aatacgttca atgtagcagc cggtttgtcg ataaataccc agttcacgca
     8281 gatttttgcc gtcggtccag gccaccagct ccgggccgac gcgataggcg cggatcaccg
     8341 gtaaataaac cttacggttg gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg
     8401 cgctggtctg taagtcctga tgctgcaatt tcggcaagta acgcgcacca acaatcaaac
     8461 tcaccagacc gattaaatag gttaaggcat acccgaggct cagattatcc agtgccagtg
     8521 agagctgcct gctttccatg ccggaatgac gcagtgtatc gccagcaccg accagaaccg
     8581 gtgtcgacgt catagagcct gctaacatac cggccgtcag gccaatatcc cagccaaaca
     8641 gcttacctaa ccctaaggcg atcaccagcg cactgccaac catcaccagt gctaacatta
     8701 ggtaattttt cccatcgcga aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga
     8761 cgcagaaaat aaacagcata aagccaagat taagcgcatc ggtgttaatg ctgaaatgtt
     8821 gttggcctaa taacagcgat acgactaaaa cgccaatgga attacccagt tggatcgaac
     8881 caagtcgtaa ctttccgaga catagcccaa gcgtgaggac cacaaataat aacagaatgt
     8941 aattcccatt taacaattcg gcgacgttta tattcacgga ggctaacttc ttgtttacta
     9001 gtaagctgtt gaaagaaatg gtaatttacg ataatgtttt ttaccagaat tcagggcgca
     9061 gattcattca gcgcacctaa acgatagtaa agtaacaata tattttacta gtgtaatcac
     9121 attaggtatc aacggctata tgaattgcgt tggcctatat tagcatggaa tgcgaagcgg
     9181 ctttatctta ctgaacgcca cactggcgaa aaatgtgttc gatagacgca gtgtcaggag
     9241 gaacgagtga aacataaaca acgttgggcg ggggcaatct gctgttttgt cctcttcatt
     9301 gtggtgtgcc tttttctggc gacgcacatg aaaggcgctt ttcgggctgc cgggcatcct
     9361 gaaatcggct tgctgttttt cattcttcct ggagcagttg ccagtttctt ttcacagcgt
     9421 agagaagtcc tgaaacctct atttggcgca atgctggcgg caccctgttc aatgctcatt
     9481 atgcggctgt ttttttcacc gacgcgctca ttctggcaag agctggcatg gttactaagc
     9541 gcggtgttct ggtgtgcgct gggggcactg tgtttcttat ttatcagtag tttgtttaaa
     9601 ccacagcaca gaaaaaatca gtaaagccct caacgcgagg gcttgtcaga cgatcaggcg
     9661 tccagatttt ctttcaccca tgcagcaaaa tcggtatagc cgccgatatg ttgctgatcg
     9721 acaaaaatct gcggcacggt ttctacgggt ttacctgcct tttgttgtag atcttcttta
     9781 gtgatccctt ccgcacgaat atctacatac tgatactgaa aatcatcgcg ttcattgctc
     9841 aatttctcag ccagatcttt tgcacgcaca cagtaagggc aacccgaacg accaaaaata
     9901 acggtttgca ttatttctct cctcatagat ttatgcctgt aatgatcacg ctaaaatgta
     9961 ttcgctgaaa gtaggtttaa cctgttgcat taattgctaa aagctataac tgttaaacac
    10021 aatacagtga aaagttttag actgaaggct cactttgcag agggaagcgt atgcgcgcga
    10081 tcggtaaatt gcctaaaggc gtgttgatac tggaatttat cggaatgatg ctactggcgg
    10141 tggcgctgct gtcggtaagc gactccctgt cgctgcctga gccattttct cggccagaag
    10201 tgcagattct gatgattttt ctcggtgttt tgctcatgct tcccgctgcg gtggtggtta
    10261 ttcttcaggt ggcaaaacgt cttgccccac agctgatgaa ccgtccaccg caatattcac
    10321 gttcagaaag agaaaaagat aatgacgcca accattgaac ttatttgtgg ccatcgctcc
    10381 attcgccatt tcactgatga acccatttcc gaagcgcagt gtgaggcgat tattaacagc
    10441 gcccgtgcga cgtccagttc cagttttttg cagtgtagta gcattattcg cattaccgac
    10501 aaagcattac gtgaagaact ggtgacgctg accggcgggc aaaaacacgt agcgcaagcg
    10561 gcggagttct gggtgttctg tgccgacttt aaccgccatt tacagatctg tccggatgct
    10621 cagctcggcc tggcggaaca actgttgctc ggtgtcgttg atacggcaat gatggcgcag
    10681 aatgcattaa ccgcagcgga atcgctggga ttgggcg
//
LOCUS       DAETQN010000142        10501 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.143, whole genome shotgun sequence.
ACCESSION   DAETQN010000142 DAETQN010000000
VERSION     DAETQN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.143"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            47..259
                     /locus_tag="K0W68_003684"
     CDS             47..259
                     /locus_tag="K0W68_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HBI3075136.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            1080..3188
                     /locus_tag="K0W68_003685"
     CDS             1080..3188
                     /locus_tag="K0W68_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBI3075137.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRI
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
     gene            3256..6798
                     /locus_tag="K0W68_003686"
                     /pseudo
     CDS             3256..6798
                     /locus_tag="K0W68_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            7779..8747
                     /locus_tag="K0W68_003687"
                     /pseudo
     CDS             7779..8747
                     /locus_tag="K0W68_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
     gene            9005..>10501
                     /locus_tag="K0W68_003688"
     CDS             9005..>10501
                     /locus_tag="K0W68_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBI3075138.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVMDGAGRRFHLVLTTQAQRAEEARKPHTASLSSPDSPCPL
                     SAPSFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAVY
                     DRSGMQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYRFEYGE
                     SRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRRT
                     EYRLHMASGKLTSVILPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDE"
BASE COUNT         2614 a         2495 c         3164 g         2228 t
ORIGIN      
        1 tattcagtaa ctttatccac aatgatgggc gtaattaatt aaattcatgt tatgtttttt
       61 gatttatatc actctcccct tcagtcagct tgtctatttc atttcctctg aaaagaaact
      121 aactattcac attgtgcaga tgtttcactt gttatcacag gtattctata atctcaaaaa
      181 gtttttaatg atggatatgc tcggagttgg agatgcaatt aatattaatg caaataaaaa
      241 tatccggcag gtatgctaaa aggataaatg gcgttctctg tatttattcg caattatcta
      301 taggataaat atggagagct tgatgagtca gagctaaaac agcaattgat aaataaatat
      361 gttgatttat tcaagaaaat ggatgtattt taaatattga ttcttaatat aacctaatca
      421 atagattatc acgtgagagc tgcacattat tggcggggcc ggggaaaaca tcattttggg
      481 aacaatgaat caggtactct taccactgaa gcaagatcat ccgggcacca ggtggtggtg
      541 cagtgctttg gaaatggtcc tctcgggggg agttgccatt attttctatg ctatttgctt
      601 attatattca tcaggtgaat ttaataaatt cctaatttga aatatccact attaagccag
      661 tgtttaacga cgttaagatg gcaatgtgca gatcatgtta tcagggtggg cggtagcgta
      721 tattccttat acaaaccatc attcatttca atgattgtta ttgaattatt tattgttgtg
      781 aataaaaaga cattatgaat attggtttga ctttgcatat caggtttttt attactcgct
      841 gtaatgtacg agcctgtcgt gaataacgtc gagaacccat ccgttgcgtt tgtgattatt
      901 ttgttgacta aacagacacc cgtttctctg aagtaaaatc ccagactaaa tcatcacata
      961 accatgacat ttttctgata ttccccggta acgccagatg tcgactcgct tatctcatcc
     1021 gcccgacatg ttgtacatgc aatatgtgcg gattattatt cattttcacg gaggttgcta
     1081 tgtcaaccgg attacgtttc acactggaag tggacggcct gccaccggat gcttttgcgg
     1141 tggtttcctt tcatttgaac cagtcactct cttcgctctt ttccctcgat ctctctctgg
     1201 tcagccagca gtttctctcc cttgaatttg cgcaggtgct ggacaaaatg gcctacctga
     1261 cggtatggca gggcgatgac gtacagcgcc gggtgaaagg tgtggtgacc tggtttgaac
     1321 tgggggagaa cgacaaaaac cagatgctgt acagcatgaa ggtatgccca ccgctgtggc
     1381 gcatagggct gcgccagaac ttccgtatct tccagaatga ggacatcgaa agcatcctcg
     1441 cgacgatcct gaaggaaaac ggtgtgaccg agtggagtcc gctgttcagc gagccgcatc
     1501 cttcccgtga gttttgtgtc cagtacggcg aaactgatta cgatttcctg tgccggatgg
     1561 cggcggagga aggcatcttc ttttatgagg agcacgcgca aaaaagtacc gaccagagcc
     1621 tggtcctgtg cgacaccgtg cgttatctgc cggagtcctt tgagatcccc tggaacccga
     1681 acacccgtac cgaggtaagt accctctgca tcagccagtt tcgctacagc gcacaaatcc
     1741 gtccttcttc cgtagtgacc aaagactaca cctttaaacg tcccggctgg ccagggcgtt
     1801 ttgatcagga aggccagtac caggattacc agcgcacgca gtatgaagtg tatgactacc
     1861 ccggacgttt caagggtgcc cacgggcaga actttgcccg ctggcagatg gacggctggc
     1921 gaaacaacgc agaagtggcg cgcggaacaa gccgttcgcc ggagatatgg ccgggacggc
     1981 gaattgtgct gacggggcat ccgcaggcga acctgaaccg ggaatggcag gtggtggcga
     2041 gcgatcttca cggcgaacag ccgcaggcgg taccgggacg caggggttca ggcaccacgc
     2101 tggagaacca ttttgcggta atcccggcag acagaacatg gcggccacag ccgttgatga
     2161 aaccgctggt ggatggcccg cagagcgccg tcgtgacggg accggcaggc gaggaaatct
     2221 tctgtgatga acatggccgc gtgcgggtga aatttaactg ggaccgctat aacccgtcaa
     2281 accaggacag ttcatgctgg atccgtgtgg cacaggcgtg ggcaggcacc ggctttggca
     2341 acctggcgat accgcgtgtg ggtcaggagg tgattgtgga cttcctcaac ggcgacccgg
     2401 accagccgat tattatgggg cgtacctacc accaggaaaa ccgcacaccc ggcagcctgc
     2461 cggggacgaa gacgcagatg accattcgtt cgaaaaccta taagggcagc gggtttaatg
     2521 aactgaagtt tgacgatgcg acagggaaag aacaggtcta catccacgcg cagaagaaca
     2581 tgaacaccga ggtgctgaat aaccgcacca cggatgtgat aaacaaccat gctgaaacca
     2641 ttggcaacaa tcagatgatt gcggttacca acaatcagat agagacggtg ggcgttaacc
     2701 agatagagac ggtgggcagt aaccagataa ttaaagtggg ttctgttcag gtggaaacga
     2761 ttggacttgt tcgtgcgctg acggtgggcg tggcgtatca gacgacggta ggtggcatta
     2821 tgaacacctc ggtggcactg atgcagtcct cgcagatcgg tttgcataaa tcgttgaggg
     2881 tcgggctggg ttatgacgtc aaagtcggaa ataacgttac cttcaccgtt ggtaaaacga
     2941 aaaaggatga taccgggcag accgcgattt actccgccgg tgagcatctg gagctctgct
     3001 gtggtaaggc aaggctggtg ctgacgaagg acggacaaat ttttctcaac ggcacaaaaa
     3061 ttcatttgca gggtaaggag caggttaatg gtgactcact gttgattaac tggaactgtg
     3121 cagcctcgaa atccccaccg aagacccctg atgaaaagca ggatacgccg gatatgagag
     3181 agtactgatc ccgtacctgt tcggtattca gtaacaaacc agcattaaaa ataaacagaa
     3241 gaaggaattt gaatcatgag cggaaaacca gcggcgcgtc agggagatat gactcagtat
     3301 ggcggtccca ttgtccaggg ttcggcaggt gtaagaattg gcgcgcccac cggcgtggcg
     3361 tgctcggtgt gtccgggcgg gatgacttcg ggcaacccgg taaatccgct gctgggggcg
     3421 aaggtgctgc ccggcgagac ggaccttgcg ctgcccggcc cgctgccgtt cattctctcc
     3481 cgcacctaca gcagctaccg gacgaagacg cctgcaccgg tgggcgtttt cggccccggc
     3541 tggaaagcgc cttctgatat ccgcttacag ctacgtgatg acggactgat actcaacgac
     3601 aacggcgggc ggagcattca ctttgagccg ctgctgccgg gggaggcggt gtacagccgc
     3661 agtgagtcaa tgtggctggt gcgcggtggt aaggcagcac agccggacgg ccatacgctg
     3721 gcgcggctgt ggggggcgct gcgccggata tccggttaag cccgcatctt tacctggcga
     3781 ccaacagcgc acaggggccg tggtggatac tggggggggc tgagcgggtg ccgggtgctg
     3841 aggacgtact gccagcgccg ctgccgccgt accgggtgct taccgggatg gcggacgctg
     3901 acgtaccggc gtgaggccgc cggtgacctg gccggggaaa tcaccgacgt gacggacggt
     3961 gccgggcggg agttccgtct ggtgctgacc acgcaggcgc agcgtgcgga agaggcccgc
     4021 acctcttcgc tatcttcttc tgacagttcc cgccctctct cagcctcagc gttccccgac
     4081 acactgcccg gtaccgaata cggccccgac aggggtatcc gcctttcggc ggtgtggttg
     4141 atgcacgacc cggcataccc ggagagcctg cccgctgcgc cactggtgcg gtacacgtat
     4201 acggaagccg gtgaactgct ggcggtatat gaccgcagca atacgcaggt gcgcgctttc
     4261 acgtatgacg cgcagcgccc gggccggatg gtggcgcacc gttacgcggg aaggccggag
     4321 atgcgctacc gctacgacga tacggggcgg gtggtggagc aactgaaccc ggcagggtta
     4381 agctaccgct atctttatga gcaggaccgc atcaccgtca ccgacagcct gaaccggcgt
     4441 gaggtgctgc atacagaagg cggggccggg ctgaaacggg tggtgaaaaa agaactggcg
     4501 gacggcagcg tcacgcgcag cgggtatgac gcggcaggaa ggctcacggc gcagacggac
     4561 gcggcgggac ggcggacaga gtacggtctg aatgtggtgt ccggcgatat cacggacatc
     4621 accacaccgg acgggcggga gacgaaattt tactataacg acgggaacca gctgacggcg
     4681 gtggtgtacc cggacgggct ggagagccgc cgggaatatg atgaaccggg caggctggta
     4741 tcggagacat cgcgcagcgg ggagacagta cgctaccgct acgatgacgc gcacagtgag
     4801 ttaccggcga cgacaacgga tgcgacgggc agcacccggc agatgacgtg gagccgctac
     4861 gggcagttgc tggcgttcac tgactgctcg ggctaccaga cccgctatga atacgaccgc
     4921 ttcggccaga tgacggcggt tcaccgcgag gaaggtatca gcctttaccg ccactatgac
     4981 aaccgtggcc ggttaacctc ggtgaaagac gcacagggcc gtgaaacgca gtatgagtac
     5041 aacgccgcag gcgacctgac tgccgttatc accccggacg gcaaccggag cgagacacag
     5101 tacgatgcgt ggggaaaggc ggtcagcacc acgcagggcg ggctgacgcg cagtatggag
     5161 tacgatgctg ccggacgtgt catcagtctg actaacgaga acggcagtca cagcgacttc
     5221 agctacgatg cgctggaccg gctggtacag cagggcggct ttgacgggcg gacgcaacgt
     5281 tatcattatg acctgaccgg aaaactcacg cagagtgagg atgaggggct tgtcaccctc
     5341 tggcactacg atgcgtcgga ccgcatcacg caccggacgg tgaacggcga cccggcagag
     5401 cagtggcagt atgacggcca cggctggctg agagaaatca gccacctgag cgaaggccac
     5461 cgtgtcgctg tccactacgg ctacgacgat aagggccgcc tgacgggcga acggcagacg
     5521 gtggagaacc cggagacggg ggaactgctg tggcagcatg agacgaaaca cgcctacaac
     5581 gaacaggggc tggcaaaccg cgtcacgccg gacagcctgc cgccggtgga gtggctgacc
     5641 tacggcagcg gttacctggc gggcatgaag ctgggcggga cgccgctggt cgagtatacg
     5701 cgggacaggc tgcaccgtga gacggtgcgc accctcgcac cggacctgga catccgcatc
     5761 ccgtatgcca ccgacccggc gggaaaccgc ctgccggacc cggagctaca cccggacagc
     5821 acgctcacgg cgtggccgga taaccgcatc gcagaggatg cgcactatgt ttaccactat
     5881 gatgaatacg gcagactgac ggagaagacg gaccgcatcc cgacgggtgt gatacggacg
     5941 gacgacgagc ggacccacca ctacgacagc cagcaccgcc tggtgttcca cacgcggata
     6001 cagcatggcg agccactggt cgagagccgc tacctctacg acccgctggg acggcgaatg
     6061 gcgaaacggg tatggcggcg ggagcgtgac ctgacggggt ggatgtcgct gtcgcgtaaa
     6121 ccggaggaga catggtacgg ctgggacggc gacaggctga cgacggtaca gaccgacacc
     6181 acacgtatcc agacggtata cgagccggga agcttcacgc cgctcatccg ggtcgagaca
     6241 gagaacggcg agcgggagaa agcgcagcgc cgcagcctgg cggagaagct gcagcaggaa
     6301 gggagcgagg acgggcacgg cgtggtattt ccggctgaac tggtgcggct gctggacaga
     6361 ctggaggaag aaatccgggc agaccgcgtg agcagtgaaa gccgggtgtg gcttgcgcag
     6421 tgcgggctga cggtggagca actggccaga caggtggagc cggaatacac accagtgcga
     6481 aacgttcatc tttaccactg cgaccatcgg ggccagccgc tggcgctcat cagcgaagac
     6541 ggcgatacgg tgtggagcgg ggcgtatgat gagtggggca accagctaaa tgaggagaac
     6601 ccgcatcatg tgtatcagcc ataccgtctg ccggggcagc agtatgatga ggagtcgggg
     6661 ctgtactaca atcgtcaccg gtactatgat ccgttgcaga gacggtacat cacccaggac
     6721 cccattgggc tgaaaggtgg gtggaatctg tatcagtatc cgctgaatcc ggtgagcagg
     6781 atagatccgt tggggttatt ctcatgtgag agttgtagtc attttataga tgatataaat
     6841 aataaactgg ataaccttcc gtttaacccg acaaaaatgt ttacagctag tttaaatact
     6901 gctaattcag caaggctgta tgcaagtggc atgacaaaat tcggtataag tgttgctgcc
     6961 gatggaacga tcattgctgc cccagtcggt gcagcttcta tggctttagg ggcgtggaat
     7021 ataaaatctg cgacagctgc atataacaga gcaaatttac aattatcaga atcacttcag
     7081 gaaaatggta gagataggtg ttggagaaat ttattgggta ttttacctta tggtgagcag
     7141 tttgatgacc ctggtgaacc atcaatatta gacgtttatg gtgaaaagat tgagaatatc
     7201 aaagattctc caatgaagtt ttttgaagag cttgggacgc tggggttgta atgtgtattt
     7261 ttaatgatat ttttatgttt gtgtttattg ttagtagtat acttgtaacc gtgcattttg
     7321 tgtattcttt attttttaaa agtggaaagg taaaagataa ggtaaaaaaa tggattaaag
     7381 taatgtgtga tttattttgg gggataggat agtggtgtga tatggataaa tataaattac
     7441 agtggctggc atatttcgac ctgacgccgg agtagataaa aaaacagaca ggattggagt
     7501 atagcaagcg tctgacaatc tctcgggaca gtagtatgat gaagaggcgg ggttgtacta
     7561 taaccgtaac cggtactacg atccgctaaa gggcaagtat atgactcagg gaaagatcat
     7621 cagaagttca ctttttgtac taaataattc gcattttatg tttaaaaatt gagatattcc
     7681 tcattacctg aagctgtttt tttattgctt atacataatc aaatactcct tacataatta
     7741 aggagaacaa aatggaaatt aaaaaattga tggaacattt tttgaagcaa tatggtgatt
     7801 ttgaaaatgg cattcctgtt cacgatacca ttgccagagt tgtatcctgt atcagtcctg
     7861 caaaatttca tgagtgcttt attaactgga tgcgtgactg ccattcttca gatgataaag
     7921 acgtcattgc aattgatgga aaaacgctcc ggcactctta tgataagagt cgtcgcaggg
     7981 gagcgattca tgtcattagt gcgttctcaa caatgtacag tctggtcctc gggcagatca
     8041 agacggatga gaaatccaat gagattacag ccattcctga acttcttaac atgctggata
     8101 ttaaaggaaa aatcatcaca actgatgcga tgggttgcca gaaagatatt gcagagaaga
     8161 tacaaaaaca gggaggtgat tatttattcg ctgtaaaagg aaaccagggg cggctaaata
     8221 aagcctttga ggaaaaattt ccgctgaaag aattaaataa tccagagcat gacagttacg
     8281 caatcagtga aaagagtcac ggcagagaag aaatccgtct tcatattgtt tgcgatgtcc
     8341 ctgatgaact tattgatttc acgtttgaat ggaaaggact gaagaaatta tgcgtggcag
     8401 tctcctttcg gtcaataata gcagaacaaa agaaagagcc agaaatgacg gtcagatatt
     8461 atatcagttc tgctgattta actgcagaaa agttcgccac agcaatccga aaccactggc
     8521 acgtggagaa taagctgcac tggcgtctgg acgtggtaat gaatgaagac gactgcaaaa
     8581 taagaagagg aaatgcagca gaattatttt cagggatacg gcacattgct attaatattt
     8641 tgacgaatga taaggtattc aaggcagggt taagacgtaa gatgcgaaaa gcagccatgg
     8701 acagaaacta tctggcgtca gtccttgcgg ggagcgggct ttcgtaatct tgccctgctg
     8761 aagagtttga aagggcacgg atcctttcaa aagaatacat tgagaaatat ataagctaaa
     8821 gatgattagc tgtatttcat gataataatc taatgaggga tttttctgaa catcaaattg
     8881 catcgaagtt agttagagtg ctgcgattgt cttgcacgta ataaattcag agagaaaata
     8941 tagttgtggg tatgagaaaa taaccaacct aatgtaaatc aattcagatg aagaggaata
     9001 aaccatgagc ggaaaaccgg cggcgcggca gggcgacatg acccgcaagg gactggatat
     9061 tgtgcagggt tcagcgggtg tgctgatagg tgcgccgacg ggagtggcct gttcggtgtg
     9121 tccgggaggg attacctatg ctaacccggt gaacccggtg ctgggtgcga aggtgctgcc
     9181 gggcgagacg gaccttgcgc tgccctgccc gctgccgttt attcttttcc gcgcctacag
     9241 cagctaccgg accagaacac ccgcgccggt gggggtgttt ggtcccggct ggaaagcgcc
     9301 cttcgatatc cgcttacaga tacgcgatga aggcctgata ctcaacgaca gcggcggcag
     9361 gagcattcac tttgagccgc tgtttcccgg cgagataagc tacagccgca gcgagtcact
     9421 ctggctggcg cggggcggag tagcggcgca gcacagttcg cagccgctaa gcgcgctctg
     9481 gcaggtgctg ccggaagatg ttcgcctgag tccgcatgtg tacctggcga caaacagcct
     9541 tcaggggccg tggtggatac tcagctggcc ggagcgggtg ccgggggcgg acgaggtgct
     9601 gccgccggag ccgcccgcat accgggtgct gacgggcgtg gtggacggct tcgggcggac
     9661 gctggccttt caccgggcag cggagggtga cgtggcgggc gcggtgacgg gggtgatgga
     9721 cggcgcgggc cgccgttttc acctggtgct gaccacacag gcgcagcggg cggaagaggc
     9781 ccgtaaaccg cacaccgctt cgttatcttc ccctgacagt ccctgccctc tctcagctcc
     9841 ttccttcccc gacacgctgc ccgccggtac agagtacggt gccgataacg gtatccggct
     9901 ggaggcggta tggctgacac acgacccggc atacccggat gaacagccga ctgcgccgct
     9961 ggcgcgctac acgtacacgg ccggtggtga actgcgggcg gtgtatgacc gcagcgggat
    10021 gcaggtgcgc gggtttactt atgatgcgga gcacgccggg cggatggtgg cgcaccatta
    10081 tgcggggagg ccggagagct gctaccggta tgatgatacc ggtcgggtga cggagcaggt
    10141 caacccggag gggctggact accgctttga gtacggagag agccgggtaa tcatcacgga
    10201 cagcctgaac cggcgggagg tgctgtacac ggaaggcgag ggtggcctga agcgggtggt
    10261 gaagaaggag catgccgacg ggagcatcac ccgcagcgag tatgatgagg cggggaggct
    10321 gaaggcgcag acggatgcgg cgggacggcg gacggagtac cgactgcata tggcgtctgg
    10381 aaagctgact tcagtgatac tgccggacgg caggacggta cggtatggct ataacagcca
    10441 gcggcaggtg acgtcggtga cgtacccgga cgggcttcgc agcagccggg agtatgatga
    10501 g
//
LOCUS       DAETQN010000143        10482 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.144, whole genome shotgun sequence.
ACCESSION   DAETQN010000143 DAETQN010000000
VERSION     DAETQN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.144"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="K0W68_003689"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="K0W68_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBI3075139.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="K0W68_003690"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="K0W68_003690"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBI3075140.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="K0W68_003691"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="K0W68_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HBI3075141.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="K0W68_003692"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="K0W68_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBI3075142.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="K0W68_003693"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="K0W68_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="K0W68_003694"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="K0W68_003694"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBI3075143.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVTAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="K0W68_003695"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="K0W68_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBI3075144.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="K0W68_003696"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="K0W68_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBI3075145.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="K0W68_003697"
     CDS             complement(9457..10149)
                     /locus_tag="K0W68_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBI3075146.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2565 a         2530 c         2772 g         2615 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tgactgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt ggcgggaaag
    10441 cgtattatgc acaccccgcg ccgctgagaa aaagcgaagc gg
//
LOCUS       DAETQN010000144        10447 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.145, whole genome shotgun sequence.
ACCESSION   DAETQN010000144 DAETQN010000000
VERSION     DAETQN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.145"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..190
                     /gene="bdm"
                     /locus_tag="K0W68_003698"
     CDS             <1..190
                     /gene="bdm"
                     /locus_tag="K0W68_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HBI3075147.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            292..429
                     /gene="sra"
                     /locus_tag="K0W68_003699"
     CDS             292..429
                     /gene="sra"
                     /locus_tag="K0W68_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HBI3075148.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            586..2283
                     /gene="maeA"
                     /locus_tag="K0W68_003700"
     CDS             586..2283
                     /gene="maeA"
                     /locus_tag="K0W68_003700"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBI3075149.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDIGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2417..3419
                     /gene="adhP"
                     /locus_tag="K0W68_003701"
                     /pseudo
     CDS             2417..3419
                     /gene="adhP"
                     /locus_tag="K0W68_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3565..3849
                     /locus_tag="K0W68_003702"
     CDS             3565..3849
                     /locus_tag="K0W68_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBI3075150.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4077..4730)
                     /gene="fdnI"
                     /locus_tag="K0W68_003703"
     CDS             complement(4077..4730)
                     /gene="fdnI"
                     /locus_tag="K0W68_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBI3075151.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4723..5607)
                     /gene="fdnH"
                     /locus_tag="K0W68_003704"
     CDS             complement(4723..5607)
                     /gene="fdnH"
                     /locus_tag="K0W68_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBI3075152.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5620..8667)
                     /gene="fdnG"
                     /locus_tag="K0W68_003705"
     CDS             complement(5620..8667)
                     /gene="fdnG"
                     /locus_tag="K0W68_003705"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8080..8082),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBI3075153.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8899..9780
                     /gene="yddG"
                     /locus_tag="K0W68_003706"
     CDS             8899..9780
                     /gene="yddG"
                     /locus_tag="K0W68_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBI3075154.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10039..>10447
                     /locus_tag="K0W68_003707"
     CDS             10039..>10447
                     /locus_tag="K0W68_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBI3075155.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGD"
BASE COUNT         2561 a         2690 c         2623 g         2573 t
ORIGIN      
        1 ttcaacagca gaacccgctc tggttaatgc cattgagcaa ggccttcggg cagagcaagg
       61 tgttgtcact gaagatgaca ttctcatgga gctgaccaaa tgggtggaag cttctgataa
      121 cgacatcctc agtgatatct accagcaaac gatcaattat gtggtcagtg gccagcaccc
      181 tacgctttaa ggtgctatgc ttgatcggca acctaattta ggggtttagc acgtgtttct
      241 tcgctacggc gatgttgtcc ttaaaactag caacaggatt gaggagttaa aatgaaatcg
      301 aaccgtcagg cacgtcatat tcttggactg gaccataaaa tttctaacca gcgcaaaata
      361 gttaccgaag gtgacaaatc cagcgtggta aataacccaa ccggcagaaa acgccccgct
      421 gaaaagtaat tcataaccat cagtcctcaa tgacgattaa acaccattgc ctgcgcaatg
      481 gtgtttttgt ttttatctgc tttatacttg gggccgacgc cctggcggta aagcaaagac
      541 gataaaagcg tgccagggat ggatattcaa aaaagagtga gtgacatgga accaaaaaca
      601 aaaaaacagc gttcgcttta tatcccttac gctggccctg tactgctgga atttccgttg
      661 ttgaataaag gcagtgcctt cagcatggaa gaacgccgta acttcaacct gctggggtta
      721 ctgccggaag tggtcgaaac catcgaagaa caagcggaac gagcatggat ccagtatcag
      781 ggattcaaaa ccgaaatcga caaacacatc tacctgcgta acatccagga caccaacgaa
      841 accctcttct accgtctggt aaacaatcat cttgatgaga tgatgcctgt tatttatacc
      901 ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct accgccgttc acgcggcgtg
      961 tttatctctt accagaaccg ccacaatatg gacgatattc tgcaaaacgt accgaaccat
     1021 aatatcaaag tgattgtggt gactgacggt gaacgtattc tggggcttgg tgaccagggc
     1081 atcggcggga tgggcattcc gatcggtaaa ctgtcgctct ataccgcctg tggcggcatc
     1141 agcccggcgt atacccttcc ggtggtgctg gatatcggaa cgaacaacca acaactgctt
     1201 aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca ctgacgacga atactatgaa
     1261 ttcgttgatg aatttatcca ggctgtgaaa caacgctggc cagacgtgct gttgcagttt
     1321 gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc gctatcgcaa tgaaatttgt
     1381 tcttttaacg atgacattca gggcactgcg gcggtaacag tcggcacact gatcgcagca
     1441 agccgagcgg caggtggtca gttaagcgag aaaaaaatcg tcttccttgg cgcaggttca
     1501 gcgggatgcg gcattgccga aatgatcatc gcccagaccc agcgcgaagg attaagcgag
     1561 gaagcggcgc ggcagaaagt ctttatggtc gatcgctttg gcctgctgac ggacaagatg
     1621 ccgaacctgc tgcctttcca gaccaaactg gtgcagaaac gcgaaaacct cagtgactgg
     1681 gataccgaca gcgatgtgct gtcactgctg gatgtggtgc gcaatgtaaa accagatatt
     1741 ctgattggcg tctcaggaca gaccgggctg tttacggaag agatcatccg tgagatgcat
     1801 aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc cgacgtcacg cgtggaagcc
     1861 acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc tggtcgccac gggcagcccg
     1921 tttaatccag tggtatggaa agataaaatc taccctatcg cccagtgtaa caacgccttt
     1981 attttcccgg gcatcggcct gggggttatt gcttccggcg cgtcacgtat caccgatgag
     2041 atgctgatgt cggcaagtga aacgcttgca cagtattcgc cgctggtgct gaacggcgaa
     2101 ggtctggtac taccggaact aaaagatatt cagaaagtct cccgtgcaat tgcgtttgcg
     2161 gttggcaaaa tggcgcagca gcaaggcgtg gcggtgaaaa cctctgccga agctttgcaa
     2221 caagccattg acgataattt ctggcaagcc gaataccgcg actaccgccg tacctccatc
     2281 taagcctgcg cccggtagtg aaggctaccg ggcaattttc ctctcccttt ttcagatctc
     2341 atccatactg ggtagtggcg aataaatctc atttgcctca cctgctatgc agaacatcat
     2401 ccgaaaagga ggaactatga aggctgcagt tgttacgaag gatcatcatg ttgacgttac
     2461 ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg ctgaaaatgg agtgttgtgg
     2521 tgtatgtcat accgatcttc atgttaagaa tggcgatttt ggtgacaaaa ccgacgtaat
     2581 tctgggccat gaaggcatcg gtgtggtggc agaagtgggt ccaggtgtca cctcattaaa
     2641 accaggcgat cgtgccagcg tggcgtggtt ctacgaagga tgcggtcatt gcgaatactg
     2701 taacagtggt aacgaaacgc tctgccgttc agttaaaaat gccggataca gcgttgatgg
     2761 cggaagagtg catcgtggtc gccgattacg cggtaaaagt gccagatggt ctggactcgg
     2821 cggcggccag cagcattacc tgtgcggggg tcaccaccta caaagccgtt aagctgtcaa
     2881 aaattcgtcc cgggcagtgg attgctatct acggtcttgg cggtctgggt aacctcgccc
     2941 tgcaatacgc aaagaatgtc tttaacgcca aagtgatcgc cattgatgtc aatgatgagc
     3001 agttaaaact ggcaaccgaa atgggtgcag atttagcgat taactcacgc accgaagacg
     3061 ccgccaaaat tgtgcaggag aaaaccggtg gcgctcactc tgcggtggta acagcagtag
     3121 ctaaagctgc gtttaactcg gcagttgatg ctgtccgtgc aggcggtcgt gttgtggctg
     3181 tcggtctgcc gccggagtct atgagcctgg atatcccacg tcttgtgctg gatggcattg
     3241 aagtggtcgg ttcgctggtc ggcacgcgcc aggatctaac cgaagccttc cagtttgccg
     3301 ccgaaggcaa agtggtgccg aaagtcgccc tgcgtccgtt agcggacatc aacaccatct
     3361 ttaccgagat ggaagaaggc aaaatccgtg gccgtatggt gattgatttc cgtcactaag
     3421 aggtctttgc tgcgactccc atgttcgggt cgcagcatcg cacactctcc aacatgaaat
     3481 ggctaaatgg attattgttg cgtaggaagg aacaataatg atagtgttcc cagagaagga
     3541 acgctactga gagtaaagac actaatgaaa atggccaatc atccccgccc gggggacatt
     3601 attcaggaat cactggacga acttaatgtc agcctgcgcg agtttgccag agcaatggaa
     3661 attgcgccct caacggcaag ccgattgctg accggaaaag cagctttgac gccagaaatg
     3721 gcaattaaac tttccgtggt gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac
     3781 gcctggagtc tggcagaggc agaaaaaacg gtggatgtgt cgcgattacg ccgtctggtt
     3841 acgcaataaa aaatggcacc ttataaagcg ccatttgaaa ctgatgacaa acgcaacatt
     3901 gcctgatgcg ctacgcttat caggcctaca cgttcacttc aatctattgt atttgaaggg
     3961 ttttcatgcc ggataaggca ttcacgacgc atccgggatg aacaaagccc acgttgtcta
     4021 caatatgaaa atggcgcctt ttacagcgcc attttcagct taaagttcaa aagagattat
     4081 atcccttctt cactctcttt tttcgcttct gccttctcga tttcacgata ccagcgcgga
     4141 tggtgtttct tcgcccagcg acggctcacc ttcccttcga tcatcccttt aatcgaccct
     4201 ttcacccaga atgccatata catatggatc aggatggcgt ggatcaggat gatacccgca
     4261 gccgcgtgga tcagcaggct gtagcgaaca acctgcatcg ggaagtactg cgcaaagtac
     4321 ggacgccaga taatcacccc ggtcaccagc agcacgaaaa tcatgctcat gatcgaccag
     4381 aacatcatct tttgcccggc gttgtactta ccaacatccg ccactttgtg ctcattgcct
     4441 tttaatactt cgacaatgtt caacagccac ggaatatctt tcttatccgg gatgttgtga
     4501 tgcacaaaac gcacaaacat aaacatcagt gcgacgaaaa tcgcaatgcc gaagaacggg
     4561 tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg tcagccattg cagcgtcggg
     4621 aagaagaacg aaatcccgga cagcgccacc aggaagaagc aaatcaccac ggtccagtga
     4681 caggcgcgat caataaattt ggtgcgcaca atcattttcg acttactcat gatgatcctc
     4741 ctcgtcatcg tccacttcct tattcgggcc aataccgatg tagtggaaaa tcaacccggc
     4801 aaaagtggca ataaagccag ccgctgccag cggtttcaac gcgcctttcc acaggcttac
     4861 cgaggtgtcg atcttcggat ctttcggcag accgtaatac agctccggct gatcggcgtg
     4921 atgcagcacg tacataacgt gcgtaccacc gacccctttc gggttgtaga cgccagcatg
     4981 ttcgtaacca cgcgctttca gtttcgccac gcgctcttcc gccagctcca gcatctcctt
     5041 cttggtgccg aagtggatag ccccggtcgg acaggttttc acacaagccg gttcctggcc
     5101 gacgctgacg cgatcgacgc agagcgtgca tttatatacc cggttatcct ctttgttgag
     5161 gcgcggaata ttaaacggac acccggcaat gcagtaacca cagccgatgc agttttccga
     5221 ctggaaatcg acaatcccgt tagcgtactg aatgattgca ccagcagacg ggcacgcctt
     5281 caggcagccg ggatcttcac agtgcataca gccgtcttta cggatcagcc actccagctt
     5341 gccgttctgt tcggtttcgc taaagcgcat caccgtccag gacttggcgc tcagatcggc
     5401 ggggttatcg taaaccccga cgcagtgccc cacttcatca cggatgtcgt tccactccga
     5461 acacgccacc tgacaggctt tacagccgat acaggtggaa acgtcgataa gttttgcgac
     5521 ttctgctttg taatcacgca cctgagaagg cggcgtgatg gagttagttg cggacctttt
     5581 gataatatcc tgcgtttcca tagccatctg ttcgccccct tacgccttct cgatgttgac
     5641 taagaacgct ttatattccg gcgtttgcga gtttgcatca ccgacattcg gcgtcagagt
     5701 gttagcgata taacctttac gcgcgacacc ctcaaagccc cagtggattg gaatacccac
     5761 cgtttcaacc tgctgaccgt ttacattcag cggtttcaga cggcgcgtta ccaccgccac
     5821 cgcgcggata aagccacgct tgcttgagac agtgacacga tcgccattat taatgccttt
     5881 cgccgccgcc agcgtttcgc tgatttccac aaactgttcc ggctgagcaa ttgcgttgag
     5941 caatgcgtgc ttggtccagg tgtggaagtg ctcggtcaga cgataggtcg tacccacata
     6001 cgggaactgc tcttttttac ccatccgcag cgcgtcttgt tcatacagac gaacaaccgg
     6061 gttagacacc acgttcggat gcagcgggtt agtgcccagc ggcgtttcaa tcggctcgta
     6121 gtgttccggg aacggacctt ccgccatttt gttgatggca aacaggcgtc ccatcccttc
     6181 cggctgcatg ataaacggcc cggttggcgt acccggtgcg gcattgccga agtccggaat
     6241 atcgttaccc gtccacttgc tgccgttcca ctggatcagc atccgtttcg gatcccacgg
     6301 tttaccattg atatccgccg aagcacggtt gtagagcacg cgacggttga gcggccacgc
     6361 ccaggcccat cccagcgtat tccccagacc ggatgggtcg gagttatcgc ggttagccat
     6421 ctggttgccc tgctctgtcc agctaccggt gtagatccag caagaagatg cggttgtacc
     6481 gtcatcacgc agatgcgcaa agctactcag caactgacct ttcttcgcaa tcagcacgcc
     6541 attagcgtca tagagatctt ccagcgcata gccgttattc tctttcgcca cttcgtcaga
     6601 ttgcggttcg tgcggctgct tgtagttcca gctcatcttc atcagcggtt ctacgccttt
     6661 accaccttcg gcctggtaca gctcgcgcag atgatggtag atacccgcca gaatttcgcc
     6721 gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag tgccactgca accagcgacc
     6781 ggagttagcg atagaaccat cttcttcggc aaagcaggtc gaaggcagac ggaatacttc
     6841 agtctgaata gacgccggat cgacatcgtt cgactcgccg tggttctgcc agaaggtaga
     6901 ggtttcagtc accagcggat cgataaccac catgtacttc agcttgctca ggcagctcac
     6961 cactttgttt ttgtccggga aggacgcaac cgggttaaag ccctggcaga agtaaccggt
     7021 gactttgcct tcgtccatca tgttgaaata cttgatgacg tcgtaggtct ggtcccactt
     7081 cggcagccag tcatagcccc agttgttctc tttctgcgcg gcatcgccat agaaagattt
     7141 catcaggcta acgaagaact tcggatagtt gctccagtag ttcacctgat cagccagcgt
     7201 cgctttcggc gtgttcgctt ccagatacga ctgcaaatca acctgttttt ctgacggaag
     7261 cgtcagataa cctggcaggc tggtagagag cagacctaag tcagtcagcc cctgaatgtt
     7321 ggagtgacca cgcaatgcgt tcacgccgcc accggccata cccatgttac cgagcagcaa
     7381 ctggatcatc gccatagtac ggatgttctg cgcacccacg gtgtgctgcg tccagcccag
     7441 cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg gaggccagca cttcacacac
     7501 tttcaggaag tcggcttttg gcgtaccgca gatgttttca acgacgtccg gcgtgtagcg
     7561 ggaaacgtgc gctttcagca ggttccacac acagcgcgga tgagtcagtg tttcatcgcg
     7621 ttttgcatag ccgttttcat cgaactgata gttccaggac gacttatcat actggcgttt
     7681 ttcagcgtcg tagccgctga acaggccgtc ttcgaaagca aaatcatcac gcaccagcag
     7741 gctggcgttg gtgtaatgtt taacgtattc ggcgttgatt ttgttgtttt cgatcaggta
     7801 gcgcaaaacg ccagacagga acgtaatgtc cgtaccggaa cgaataggtg cgtaaatatc
     7861 cgccacagaa gcggtacgcg taaaacgagg atcgacaacg atcaaggttg catcgttgtt
     7921 gtttttcgct tccatcgccc agcggaaacc gacgggatgc gcttcagcag cgttaccgcc
     7981 cattaccatt acgacgttag cgtttttgat atccacccag tggttggtca tcgcaccgcg
     8041 accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt cagacgcgcg cctggttgtc
     8101 taccgccagc atcccgaggg agcgggcaaa tttctgggtc agcatcccgg tttcgttgct
     8161 ggcaccggag gcacacagca taccggtaga aagccaacgg tttaccgtta cgccctgctc
     8221 gttcttttca ataaagttag cgtcacggtc agctttcatc agcttcgcaa tacgggagaa
     8281 tgcttcttcc cagctaatgc gctgccattt gtcagaacct ggcgcacgat attccgggta
     8341 gcgcagacgg ttttcgctgt tgacgtaatc cagcaaaccg gccccttttg ggcacagcgc
     8401 accacggctt accggatgat ccgggtcacc ttcaatgtga taaatcgctt ctctggcgtt
     8461 tttcgcgcca tcacccaggc tatacatcaa tagcccgcaa cctacggaac agtatgtgca
     8521 ggtgttacgg atctctttag cgcgtaataa tttgtagttt cgcgcctgag ccagtgcttg
     8581 cttcggggca aagcccaatg ccgctaccgt tgttccagcc ataccgcccg cgcagatttt
     8641 aaaaaattgt ctgcgactga cgtccattgc tttcctcttt tttcaggggt attactgcgc
     8701 gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa atcaaaaaga acacgtgtat
     8761 gccacaaaat aatccgcctg cgcaaattaa cctacctcaa tagcggtaga aaaacgcacc
     8821 actgcctgac aggccagtta aaaaaatgct ataaaattca gcttaatttt taacggcaag
     8881 agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct aatagcgatc
     8941 gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct cggtccggtc
     9001 ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac ggttggattt
     9061 ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt attcgtcagc
     9121 tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca ggcgattgaa
     9181 gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat tctgtttaat
     9241 ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt cggcgtctgc
     9301 tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa tatcaccacc
     9361 agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc ctattgcaca
     9421 gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct gctaacggga
     9481 gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt tagcacgccc
     9541 gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatgc tgcatggaat
     9601 gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt tacgcctgta
     9661 ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc gttctggcaa
     9721 ggcgcgctaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg tcgtggttaa
     9781 aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa tattaaaaaa
     9841 taaatttcaa ataccagtaa ttcaccttat atctcatttc gacttcgatc acatatcgat
     9901 aaataatatt atttcatcta tttttctcat tgtttttaat caccattcta ttttaaaaac
     9961 agtatgtcat ttataaatga cgccgtttaa aattcgttta gaaaatacgt ttaacgataa
    10021 ttaaaaggat tctgttaaat gaaattaaaa atagttgcgg tggttgtaac tggtttgtta
    10081 gctgcgaacg tagcacacgc tgccgaagtc tataacaagg atggtaataa actcgacctt
    10141 tatggcaagg ttaccgctct acgttatttt actgatgata agcgtgacga tggtgataaa
    10201 acttatgccc gtctcggctt taaaggagaa acgcaaatca atgatcaaat gattggtttt
    10261 ggtcactggg aatatgattt taaaggctat aacgatgaag ccaacggctc gcgcggcaac
    10321 aagacccgtc tggcctatgc tggtttaaaa attagtgaat ttggctctct ggactatggt
    10381 cgtaactacg gtgtcggcta cgacattggt tcatggaccg atatgttgcc agaatttggt
    10441 ggcgata
//
LOCUS       DAETQN010000145        10340 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.146, whole genome shotgun sequence.
ACCESSION   DAETQN010000145 DAETQN010000000
VERSION     DAETQN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10340
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.146"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(41..1045)
                     /gene="cra"
                     /locus_tag="K0W68_003708"
     CDS             complement(41..1045)
                     /gene="cra"
                     /locus_tag="K0W68_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBI3075156.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1127..1213)
                     /locus_tag="K0W68_003709"
     CDS             complement(1127..1213)
                     /locus_tag="K0W68_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBI3075157.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1225..1716)
                     /gene="ilvN"
                     /locus_tag="K0W68_003710"
     CDS             complement(1225..1716)
                     /gene="ilvN"
                     /locus_tag="K0W68_003710"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBI3075158.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1719..3443)
                     /gene="ilvI"
                     /locus_tag="K0W68_003711"
     CDS             complement(1719..3443)
                     /gene="ilvI"
                     /locus_tag="K0W68_003711"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBI3075159.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3562..3606
                     /locus_tag="K0W68_003712"
     CDS             3562..3606
                     /locus_tag="K0W68_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075160.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3761..4705)
                     /gene="leuO"
                     /locus_tag="K0W68_003713"
     CDS             complement(3761..4705)
                     /gene="leuO"
                     /locus_tag="K0W68_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBI3075161.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5365..5451
                     /gene="leuL"
                     /locus_tag="K0W68_003714"
     CDS             5365..5451
                     /gene="leuL"
                     /locus_tag="K0W68_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBI3075162.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5544..7115
                     /gene="leuA"
                     /locus_tag="K0W68_003715"
     CDS             5544..7115
                     /gene="leuA"
                     /locus_tag="K0W68_003715"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBI3075163.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDITTAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7115..8206
                     /gene="leuB"
                     /locus_tag="K0W68_003716"
     CDS             7115..8206
                     /gene="leuB"
                     /locus_tag="K0W68_003716"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBI3075164.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8209..9609
                     /gene="leuC"
                     /locus_tag="K0W68_003717"
     CDS             8209..9609
                     /gene="leuC"
                     /locus_tag="K0W68_003717"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBI3075165.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9620..10225
                     /gene="leuD"
                     /locus_tag="K0W68_003718"
     CDS             9620..10225
                     /gene="leuD"
                     /locus_tag="K0W68_003718"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBI3075166.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
BASE COUNT         2713 a         2717 c         2521 g         2389 t
ORIGIN      
        1 ccagagggaa attcacctgg cgcgtatttt tgttcgcggc ttagctacgg ctgagcacac
       61 cgcggcgata gagattacgt ttaatgcgcg ttaaaccagg ttttggctta cgcggttcgt
      121 ccaggcttgc caggacaatc tccagcacac gctctgcgac atcgcggtga cgttgagcca
      181 ctgccagcac cgggcactgt aagaagtcga gcagttcgtt atcgccaaag gtggcaattg
      241 ccaggtcaga aggcagttta ccatcgcgac gtagcgtgac atccatcacc ccttgcaaca
      301 acgcaaacga cgttgtgaac agcgcctgcg gcatcggatg cgtttccagc catttttcga
      361 ataactgggc agccacctcc cgctcatagc tgttggcata caggaaatgc acttcgcgcg
      421 gatcatcttt ccaggcagta cggaaacctt gttcacgcag gaagctgaca gaaagctccg
      481 gcagcgcgcc aagataaagc accgtctcgg cgggaaactt acgtaactct tccgccagca
      541 tttcggcatc atcctgatcg gcaccaacca cgctggtgaa gtgttcacga tcgagggcgc
      601 ggtccagcgc gacaatcggg aacgggtcgt tagcccagcg ttgataaaaa ggatgctcag
      661 gaggcaacga cgtcgaaaca ataatggcat cgacctgacg ctgtaaaagg tgctcaatac
      721 agcgcatttc gttgtctggc tgatcttctg agcaggcaat tagcagttga taaccccgtt
      781 gccgcgcctg gcgttcaaga tagttagcga tgcgggtata gctggtgttc tccagatcgg
      841 ggatcacaag accaatagaa cgtgtgcgtc cagcacgaag cccagctgcc acggcgttcg
      901 ggtggtaatt gtgctcacgc accacagcca tgactttttc aacggttttg tcgctcacac
      961 ggtattgctt cgctttgccg ttaataacat agcttgcagt ggtccgcgac actcccgcca
     1021 gccgagcgat ttcatccagt ttcacaattg ccccttgcgt aaaatgtaaa aaccatagcc
     1081 attgtactgg tatgggttaa attcattaac atctaagcgc ataaaattaa ctgcggcaac
     1141 tgcttttatt ccaggttccc actgatttcg caaaaaaagc ccaacgtgac atgttgggct
     1201 gcaaattgcg cattcagatc attctcaacg cattatttta tcgccgcgcg aaagtccgac
     1261 cacaccagaa cgagcaactt ccacaatttt cgccacctcg cgaatcgatg ctaaaaatgc
     1321 atcaagctta tcgctggtgc ctgctaattg aacggtataa agcgagggtg tgacatcgat
     1381 aatttgccca cggaatattt ccgtattacg tttcacttcg tcacgcccgt aaccgctggc
     1441 ctgaattttc accagcatga tttcccgctc aacatgcgcg ccctgcccca actcactcac
     1501 gcgcaagaca tcgaccagct tgtgtaattg cttttcgatc tgctcaagta ctttttcatc
     1561 gcccacggtc tggatggtca tacgcgataa tgtcggatca tcggttgacg caacggtcag
     1621 gctttcaatg ttgtagccac gctgggaaaa aaggccaatc acgcgggata acgcgcctga
     1681 ttcattttcg agtaagactg ataatatccg gcgcataatc aggttctctc cgttttgctt
     1741 aaccacattt catccattcc gcccccgcga atctgcatcg ggtagacgtg ctcgctgcca
     1801 tcgacggtaa catcaacaaa caccaggcga ttattgtgca cctgttccag cgcctcgcta
     1861 agtttgcttt ccagctcatg cggatgagaa atctggatcc cgacgtgacc ataggcttcc
     1921 gccagacgga cgaaatcggg tagcgattgc atataagatt gtgaatgacg gccggaatag
     1981 atcatgtcct gccactgctt caccatcccc agatagcggt tattgagatt caccaccagt
     2041 acgggcaact cgtattgcaa cgcggtagac agttcctgga tgttcatctg aatactgccg
     2101 tcgccagtga cgcaaaccac ggtttcttct ggcaacgcca ttttgacgcc cagtgccgca
     2161 ggtaaaccaa aacccatcgt gccgaggcca ccggaattga tccagcgacg cggtttgtcg
     2221 aatggataat aaagtgcagc aaacatctgg tgctgcccga catcggacgt cacgtaagcg
     2281 tctcccttcg tcaaccgcca aagagtctcg atcaccgcct gcggtttaat cttttcactg
     2341 tgagtgtcat atttcaggca ctgacgagcg cgccactgtt caatttgctg ccaccagtcg
     2401 cggatctcat ccagtggttg atgggcggat tcttgcgaca agagttcaag catttgttcg
     2461 aggacctggc gagcatcccc cacaatcggg atatccgcag tcacggtttt agaaatggaa
     2521 gtaggatcaa tatcgatatg cagaacagtg gcatttgggc agtactttgc cagattgttc
     2581 gtcgttcggt catcaaatcg taccccgacg gcgaaaatca catccgcgtt atgcatcgtc
     2641 atattggctt cgtaggtacc gtgcattccc agcatgccca gtgcctgacg atgcgttgcc
     2701 ggaaacgctc ccagccccat caatgaggaa acaacgggca gattcaacgc ctccaccgtt
     2761 tctttcaact gctgatggca gcccgccgtg attgccccac cgcctacgta gacaaccggt
     2821 ttttttgccg ctaccagcgt ttgcagagcg cgcttaattt gccctttatg tccggtagta
     2881 gtgggattgt aagaacgcat actgaccgac tccggccaga catagggtaa tttgttcgcc
     2941 ggattaagaa tatctttcgg taaatcaacg actactggtc caggacgacc acttgccgcc
     3001 agccagaaag cctttttcag cacctgcgga atgtcttccg tttgcttaac cagaaaactg
     3061 tgtttaacca ctggtcgcga aatccccacc atgtcgcact cctgaaaggc atcgtaacct
     3121 atcaacgagg tcgctacctg cccggaaagg acaactaatg gaatggaatc catataagcg
     3181 gtggcgatgc cagtaatcgc attggtcgcc cctggacccg acgttaccag cacgacgccg
     3241 acttccccgg tcgcgcgcgc cagaccatcg gccatatgca ctgccgcctg ctcatgacga
     3301 actaaaacat gatcgatacc acccacggta tgcaatgcat cgtaaatatc aaggactgcg
     3361 cctccgggat aaccgaatac ttgtttaacg ccctgatcga taagcgatcg gacgaccatc
     3421 tcggctccag acaacatctc catggcctgc ctcactgttt gacggaaaaa atgtgtaaaa
     3481 cacattaacc gtccgatctt gcttaagcaa ttggcagcca gagacataaa cgcagggtaa
     3541 taagaataaa caggaggaaa ggtgaaaaga aacgataaaa tcctccatta cgaaggatta
     3601 aattgataat aaatcggctg aatcccacaa cttacagcaa tttctctatt tttttaatta
     3661 atcctaaaat agagagccca cacattcaga ataaaattct acattcaata aaaaatacag
     3721 aataaaccag acattcatgt ctgacctatt ctgcattcag ttagcgtttg caaattgaga
     3781 ctaattgctc ttccatccac tgatggcctt tatcgcgccc ggcagcttca tgccaggaga
     3841 gataacaggt tctgctattt tgttttaacg gcagcggtaa tacctgtaat tctaaggatt
     3901 cagcgaactc ttcagccagc caacgcggcg caatagcgac caaatgcgtt tgcgacacca
     3961 cgctaagtac gctcatcatt gccatgccct gatacgcgat actggcttgc ttatctaccg
     4021 tgtcatacca aggttgacta aatgacgcga aacgatcgag cgaaaccgcc gcatgttgtt
     4081 cgttataaac atcatgtttc agtaacgggc ccttaattgt tggatgattt ttgctggcta
     4141 ccagcaccat ttcatcttta aataatggca cgctggtaaa ttcaggacga tggaagtctt
     4201 cataactaat cacaaactcc gtttcctgat aacgcagctg atgttcagtg ttctgattta
     4261 atgaagactt gaacataaca tgtatatttg gcgcaatctg ctcaatgtga ttataaatct
     4321 gcgaggtcag aatgctgtct aacgggctgc aaacacaaag atgaaataca cgttcactgc
     4381 tcgcgggttc aaaacctgaa ccaggcaatt cattttgtac tagttgcaat gcctgacgaa
     4441 ctgagccaaa aagttgaaat gcgcgagcag tcggttgaat accacggcca taacgaacaa
     4501 aaagctcgtc attaaacatc accttcaggc gtgcaacagc gttactgacc gcaggttgcg
     4561 acatacccag aacatgagcg gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa
     4621 cggttaataa gttgagatcg accatgcgta gctgtggctt gcttaactcc gccgtctctg
     4681 aatgatctgt ttgtacctct ggcatactta actccactgt cacacttaac tccctttccc
     4741 ttattggaat gcagagaaat aatcctgaag aatatgattt atcatgcata taaaataaga
     4801 aaaagcaaaa tgagtaaaat tcggaaaaca taaagatacg ggttctgata aaaacagaat
     4861 ccattgccgt ggaatgagtc atttaccaca gcataagaat ccataatgtt tttgttgcga
     4921 aaacaatcta atcataacta ccgcgaatac tcaatcatct acaaaatgga ttaaatgtgc
     4981 ttatttaata attaatttat atatttaatg cattaattct taacattaat tgatcaataa
     5041 tattcaccaa atcaatatca aaaaaaatcg caaaacatat aattcaatac aaatcatcag
     5101 gataggtttt gcaacgcgtg cattttgtcc cctttttcct cgttgattag atgcaaaaat
     5161 ttatgctgaa atatgtcaac cgatgaaaag cgtcggtagt taagcagaaa ttaatatcgc
     5221 ttactttaac caccgcagca caattagcta attttacgga tgcagaactc acgctggcgg
     5281 gacgttttta ttgcgtcagg gttgacatcc gtttttgtat ccagtaactc taaaagcata
     5341 tcgcattcat ctggagctga tttaatgact cacatcgttc gctttatcgg tctactacta
     5401 ctaaacgcat cttctttgcg cggtagacga gtgagcggca tccagcatta agccagcacg
     5461 cagtcaaaca aaaaacccgc gccattgcgc gggttttttt atgcccgaag cgaggcgctc
     5521 taaaagagac aaggacctaa accatgagcc agcaagtcat tattttcgat accacattgc
     5581 gcgacggtga acaggcgtta caggcaagct tgagtgtgaa agaaaaactg caaattgcgc
     5641 tggcccttga gcgtatgggt gttgacgtga tggaagtcgg tttccccgtc tcttcgccgg
     5701 gcgattttga atcggtgcaa accatcgccc gccaggttaa aaacagccgc gtatgtgcgt
     5761 tagctcgctg cgtggaaaaa gatatcgacg tggcggccga atccctgaaa gtcgccgaag
     5821 ccttccgtat tcataccttt attgctacct ccccaatgca catcgccacc aagctgcgca
     5881 gcaccctgga cgaggtgatc gaacgcgcta tctatatggt gaaacgcgcc cgtaattaca
     5941 ccgatgatgt tgaattttct tgcgaagatg ccgggcgtac acccattgcc gatctggcgc
     6001 gagtggtcga agcggcgatt aatgccggtg ccaccaccat caacattccg gacaccgtgg
     6061 gctacaccat gccgtttgag ttcgccggaa tcatcagcgg cctgtatgaa cgcgtaccta
     6121 acatcgacaa agccattatt tccgtacata cccacgacga tttaggcctg gcggtcggca
     6181 actcactggc ggcggtacat gccggtgcac gccaggtgga aggtgcaatg aacgggatcg
     6241 gcgagcgtgc cggtaactgt tcgctggaag aagtcatcat ggcgattaaa gttcgtaagg
     6301 atattctcaa cgtccacacc gccattaatc accaggagat atggcgcacc agccagttag
     6361 ttagccagat ttgtaatatg ccgatcccgg caaacaaagc cattgttggc agcagcgcat
     6421 tcgctcactc ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa aactacgaaa
     6481 tcatgacacc agaatctatt ggtctgaacc aaatccagct gaatctgacc tctcgttcgg
     6541 ggcgtgcggc ggtgaaacat cgcatggatg agatggggta taaagaaagt gaatataatt
     6601 tagacaattt gtacgatgct ttcctgaagc tggcggacaa aaaaggtcag gtgtttgatt
     6661 acgatctgga ggcgctggcc ttcatcggta agcagcaaga agagccggag catttccgtc
     6721 tggattactt cagcgtgcag tctggctcta acgatatcac caccgccgcc gtcaaactgg
     6781 cctgtggcga agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc gatgccgtct
     6841 atcaggcaat taaccgcatc actgactata acgtcgaact ggtgaaatac agcctgaccg
     6901 ccaaaggtca cggtaaagat gcgctgggtc aggtggatat tgtcgccaac tacaacggtc
     6961 gccgcttcca cggcgtcggc ctggccaccg atattgtcga gtcctccgcc aaagccatgg
     7021 tgcacgtact gaacaatatc tggcgtgccg cagaagtcga aaaagagttg caacgcaaag
     7081 ctcaacacaa cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc atattgccgt
     7141 attgccgggg gacggtattg gtccggaagt gatgacccag gcgctgaaag tgctgaatgc
     7201 cgtgcgcaac cgctttgcga tgcgcatcac caccagccat tacgatgtag gcggcgcagc
     7261 cattgataac cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg agcaagccga
     7321 tgccgtgctg tttggctcgg taggcggccc gaagtgggaa catttaccac cagaccagca
     7381 accagaacgc ggcgcgctgc tgcctctgcg taagcgcttc aaattattca gcaacctgcg
     7441 cccggcaaaa ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag acattgccgc
     7501 taacggcttc gacatcctgt gcgtgcgcga actgaccggc ggcatctatt tcggtcagcc
     7561 aaaaggccgc gaaggtagcg gacaatatga aaaagcgttt gataccgagg tgtatcaccg
     7621 ttttgagatc gagcgtattg cccgcatcgc gtttgaatct gctcgtaaac gtcgccacaa
     7681 agtcacctca atcgacaaag ctaacgtgct gcaatcctct attttatggc gtgaaatcgt
     7741 caacgagatc gccacggaat acccggatgt cgaactggcg catatgtaca tcgacaacgc
     7801 caccatgcag ctgattaaag atccatcaca gtttgacgtt ctgctgtgct ccaacctgtt
     7861 tggcgacatt ctgtctgacg agtgcgcaat gatcactggc tcgatgggga tgttgccttc
     7921 cgccagcctg aacgagcaag gttttggtct gtatgaaccg gcaggcggct cagcaccaga
     7981 tatcgcaggc aaaaacatcg ccaacccgat tgcgcaaatt ctgtcgctgg cactgctgct
     8041 gcgctacagc ctggatgccg atgatgcggc ttgcgccatt gaacgcgcca ttaaccgcgc
     8101 attagaagaa ggcattcgca ccggagattt agcccgtggc gctgccgccg ttagtaccga
     8161 tgaaatgggc gatatcattg cccgctatgt ggcagaaggg gtgtaatcat ggctaaaacg
     8221 ttatacgaaa aattattcga cgctcacgta gtgtacgaag ccgaaaacga aaccccgctg
     8281 ttatatatcg accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt caatggtctg
     8341 cgcgcccacg gtcgcccggt acgtcagccg ggcaaaacct tcgccactat ggatcacaac
     8401 gtctctaccc agacgaaaga cattaatgcc tgtggtgaaa tggcgcgcat ccagatgcag
     8461 gagctgatta aaaactgcaa agaatttggc gtcgaactgt atgacctgaa tcacccgtat
     8521 caggggatcg tccacgtaat ggggccggaa cagggcgtca ccttgccggg gatgaccatt
     8581 gtctgcggcg actcgcatac cgccacccac ggcgcgtttg gcgcactggc ctttggtatc
     8641 ggcacttccg aagttgaaca cgtactggca acgcaaaccc tgaaacaggg ccgcgcaaaa
     8701 accatgaaaa ttgaagtcca gggcaaagcc gcgccgggca ttaccgcaaa agatatcgtg
     8761 ctggcaatta tcggtaaaac cggtagcgca ggcggcaccg ggcatgtggt ggagttttgc
     8821 ggcgaagcaa tccgtgattt aagcatggaa ggtcgtatga ccctgtgcaa tatggcaatc
     8881 gaaatgggcg caaaaaccgg tctggttgca ccggacgaaa ccacctttaa ctatgtcaaa
     8941 ggccgtttgc atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta ctggaaaacc
     9001 ctgcaaaccg acgaaggcgc aactttcgat accgttgtca ctctgcaagc agaagaaatt
     9061 tcaccgcagg tcacctgggg caccaatcca ggccaggtga tttccgtgaa cgacaatatt
     9121 cccgatccgg cttcgtttgc cgatccggtt aaacgcgcgt cggcagaaaa agcgctggcc
     9181 tatatggggc tgaaaccggg cattccgctg accgaagtgg ctatcgacaa agtgtttatc
     9241 ggttcctgta ccaactcgcg cattgaagat ttacgcgcgg cagcggagat cgccaaaggg
     9301 cgaaaagtcg cgccaggcgt gcaggcactg gtggttcccg gctctggccc ggtaaaagcc
     9361 caggcggaag cggaaggtct ggataaaatc tttattgaag ccggttttga atggcgcttg
     9421 cctggctgct caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg cgaacgttgt
     9481 gcatccacca gcaaccgtaa ctttgaaggc cgccaggggc gcggcgggcg cacgcatctg
     9541 gtcagcccgg caatggctgc cgctgctgct gtgaccggac atttcgccga cattcgcaac
     9601 attaaataag gagcacacca tggcagagaa atttatcaaa cacacaggcc tggtggttcc
     9661 gctggatgcc gccaatgtcg ataccgatgc aatcatcccg aaacagtttt tgcagaaagt
     9721 gacccgtacg ggttttggcg cgcatctgtt taacgactgg cgttttctcg atgaaaaagg
     9781 ccaacagcca aacccggact tcgtgctgaa cttcccgcag tatcagggcg cttccatttt
     9841 gctggcacga gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct gggcattgac
     9901 cgactacggc tttaaagtgg tgattgcgcc gagttttgct gacatcttct acggcaatag
     9961 ctttaacaac cagctgctgc cggtgaaatt aagcgatgca gaagtggacg aactgtttgc
    10021 gctggtgaaa gccaatccgg ggatccattt cgacgtggat ctggaagcgc aagaggtgaa
    10081 agcgggagag aaaacctatc gctttaccat cgatgccttc cgccgccact gcatgatgaa
    10141 cggtctggac agtattgggc ttaccttgca gcacgacgac gccattgccg cttatgaagc
    10201 aaaacaacct gcgtttatga attaatcccc ttgcccggtc aaatgaccgg gctttccgct
    10261 atcgtccacg tcatcaaacg tctttaacct ttgcggttaa aaataatgcg acaacagaaa
    10321 taaccgcaat tacccagtag
//
LOCUS       DAETQN010000146         9896 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.147, whole genome shotgun sequence.
ACCESSION   DAETQN010000146 DAETQN010000000
VERSION     DAETQN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.147"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(113..1108)
                     /gene="gapA"
                     /locus_tag="K0W68_003719"
     CDS             complement(113..1108)
                     /gene="gapA"
                     /locus_tag="K0W68_003719"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBI3075167.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1450..1863
                     /gene="msrB"
                     /locus_tag="K0W68_003720"
     CDS             1450..1863
                     /gene="msrB"
                     /locus_tag="K0W68_003720"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBI3075168.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1905..2177
                     /locus_tag="K0W68_003721"
     CDS             1905..2177
                     /locus_tag="K0W68_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBI3075169.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2338..2979)
                     /gene="pncA"
                     /locus_tag="K0W68_003722"
     CDS             complement(2338..2979)
                     /gene="pncA"
                     /locus_tag="K0W68_003722"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBI3075170.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2990..4006)
                     /gene="ansA"
                     /locus_tag="K0W68_003723"
     CDS             complement(2990..4006)
                     /gene="ansA"
                     /locus_tag="K0W68_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBI3075171.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4173..6029)
                     /gene="sppA"
                     /locus_tag="K0W68_003724"
     CDS             complement(4173..6029)
                     /gene="sppA"
                     /locus_tag="K0W68_003724"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBI3075172.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6190..6741
                     /locus_tag="K0W68_003725"
     CDS             6190..6741
                     /locus_tag="K0W68_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBI3075173.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6858..7901
                     /gene="selD"
                     /locus_tag="K0W68_003726"
     CDS             6858..7901
                     /gene="selD"
                     /locus_tag="K0W68_003726"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBI3075174.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7906..9867
                     /gene="topB"
                     /locus_tag="K0W68_003727"
     CDS             7906..9867
                     /gene="topB"
                     /locus_tag="K0W68_003727"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBI3075175.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2454 a         2472 c         2633 g         2337 t
ORIGIN      
        1 aacaagtgag cgtttccgga ttcttacaca gccactggat cggtcaactg atccttaact
       61 gatcggcatt acgaccgaag tcgctctttt tagatcacag tttcatctca acttatttgg
      121 agatgtgagc gatcaggtcc agaactttgt tggagtaacc ggtttcgttg tcgtaccagg
      181 ataccagttt cacgaagttg tcgttcagag cgataccagc tttagcatcg aacacggaag
      241 tgcaaacttc gccgttgaaa tcggtagata ctacgtcatc ttcggtgtag cccagaacgc
      301 ctttcatttc gccttcagca gcggctttaa cggcagcttt gatctgctcg taagttgcag
      361 ctttttccag acgaacggtc aggtcaacta cggatacgtt cggggtcgga acgcggaacg
      421 ccataccagt cagtttgcca ttcagttctg gcagtacttt acctacagct ttagcagcac
      481 cggtagagga cgggatgatg ttctgggaag cgccgcggcc gccgcgccag tctttgtgag
      541 acgggccatc aacggttttc tgagtagcgg tagtagcgtg aacggtggtc atcagacctt
      601 cgatgatgcc gaagttatcg ttgataactt tagccagcgg agccaggcag ttggtggtgc
      661 aggaagcgtt ggaaacgatg tcctggccag catatttgtc gaagttagcg cctttaacga
      721 acatcggagt gttgtcttta gacggaccag tcataaccac tttcttcgca ccagcggtga
      781 tgtgtttacg agcagtttcg tcagtcagga acagaccagt tgcttcagcg acaacgtcaa
      841 caccaacttc gtcccatttc aggttagccg gatcacgttc agcggtaaca cggatttttt
      901 taccgttaac gatcagatga ccgtctttca cttcaacggt accgtcgaaa cggccgtgag
      961 tggagtcata tttcagcatg tatgccatgt aatcagcgtc taacaggtcg ttgattgcaa
     1021 cgatctcgat gtcagaacgt ttctgagcag cacggaaaac aatgcgaccg atacggccaa
     1081 aaccgttgat acctactttg atagtcatat attccaccag ctatttgtta gtgaataaaa
     1141 ggttgcctgt aaaattacaa aaaccttacg cagcgtcaag cggaatcgtg tcaatcattg
     1201 cgacaaatca atcctgtgcc taagcattac gcgactgact cgcctcactc ttcctttggg
     1261 cttgaaacca catggggtcg gcgccccgaa ttttaaaggg caattacgat aaaaatgtga
     1321 ttttcatcac gatttaggtg cagccatttc gtcgagatca agtttaggac aaaattttgc
     1381 cagttggtgt taatgttttg ttagaatcag tcacgtaatg tgagcacgat taaagtgaga
     1441 tgtgagcaaa tggctaataa accttcggca gaagaactga aaaaaaattt gtccgagatg
     1501 cagttttacg tgacgcagaa tcatgggaca gagccgccat ttacgggtcg tttactgcat
     1561 aacaagcgtg acggcgtata tcactgtttg atctgcgatg ccccgctgtt tcattcccaa
     1621 accaagtatg attccggctg tggttggccc agtttctacg aaccggtgag tgaagaatcc
     1681 attcgttata tcaaagactt gtcacatgga atgcagcgca tagaaattcg ttgcggtaac
     1741 tgtgatgccc atctggggca tgtcttccct gacgggccgc agccaacggg cgaacgttat
     1801 tgtgttaact ctgcttcttt acgcttcact gatggcgaaa acggcgaaga aatcaacggt
     1861 tgaaaaaacg attcagcaac ttattccaca ggagcggaaa aattatgaat cttgatgaca
     1921 ttatcaacag catgacgcct gaggtatacc agcgtttgtc gaccgccgtt gaactgggga
     1981 aatggcccga tggcgttgcg ttgaccgagg aacaaaaagc gaactgcctg caactggtga
     2041 tgctgtggca agctcgccat aataccgaag cacagcatat gacaattgac acaaatggtc
     2101 agatggtcat gaagagcaaa cagcagttaa aagaagattt tggtatctca gcaaagccta
     2161 ttgctatgtt taagtgaatg agttatctag tatttaagcc tgttattaag ggagggcaaa
     2221 tatacttgtt aggacgtcgt gttttagctg caagtaggtt aaatgcaaca ctgacaagta
     2281 ttatctggcc tacaatgtca ttgaggaaat gtaggccaga gatgaattca actaatatta
     2341 cccctgtgtc tcttcccagt ctgccagcgt atatagcgtt gccccagctg ctgacatctc
     2401 cataaacgcg tgcgcactgt cctggggctg gatattcacg ccacgacaac catcggtaat
     2461 cacatttacc ttataaccta actgtaacgc gtccagcacg gtaaacttca cgcaatagtc
     2521 agtagccagg cccataacga tcaattcatc gatttcatga tcgcgtaacc agtcatcgag
     2581 cgaggttttc tgccgacggc cgttatcaaa aaaggcactg taactgtcaa ctaaaggatt
     2641 ttcgccttta tggaacaccg ctgcgatcgc tttttggttc agtaacggat gtaattgtgc
     2701 gccttcactg ttctgcacac agtgatctgg ccagaaggtt tgtggcaaac cgtcgagttg
     2761 gcctggcgta taaggctcta caccgtgctg actggcaaaa ctgccgtgat tcgccgggtg
     2821 ccagtcctga ctggcgataa ccgcttcacc gcgcgactgg caccagtcaa tcaggcggtt
     2881 agcgacatcc accgtactgt caccttccgg cacggcgagc gcgccaccag cacagaaatc
     2941 attttgtaaa tcgaccagta acagggcgcg agggggcatt acagtctcct taatcatccg
     3001 gcgtcagttc gccgcgcagg ttttggctca tggccttgcg aatggtttca gtatccagtt
     3061 cctggctcag caggtaatgc agtttggtta gcgtggcttc tacagtcata tctgcaccgc
     3121 caataacgcc ggcatgggcg agggcgttac cggtggcata accacccata ttcactttac
     3181 cggacataca ttgtgtcagg ttgaccacca caataccgcg atcgctggct tcttgtaatt
     3241 cctgcaggaa ggctttgttt tgtggcgcat tacccacgcc ataggagcgc agaatcaatg
     3301 ctttcaccgg ttggcgcaga aaattgcgca cgacgtcagc agaaatccct ggataaatcg
     3361 tcactacgcc aattggttgt ggcgtgattg gatgaacgat taattcccct tcaccgtgcg
     3421 gggcgggtgg cgtattcaaa cgacgaatat ggataccagc ttccagtaac ggaggaaggt
     3481 ttggagaggc aaacgcatca aaaccatcgg catgggcttt ggtagtgcgg ttgccgcgat
     3541 acaatcggtt attgaaaaag agcgttactt cgttgatcgg ataattcgcc gcaacgtaca
     3601 acgcattcag cagattaatt tgtccgtcag agcgtaactc agccagcggg atttgtgacc
     3661 ctgtcacaat gaccggttta ccgagattct cgagcatgaa cgacagcgca gaggcagtat
     3721 acgccatcgt gtcggtgccg tgcaggatga caaaaccatc atagtcgtca tagtgcgctt
     3781 taatatcttc agcaatatgc tgccagtctt ctggcgtcat atcagaagaa tccatcagcg
     3841 gcgtatattc atgaatggtg aaatctggca tctccgggcg atggaattcc ggcatcagcg
     3901 ccagttggcg ttgtagatga cctgacaccg gaatataacc ctgctcggaa cgctgcatcc
     3961 cgatggtccc gcccgtgtag gcgacgtaaa ttgatttctt ttgcatgata ttgatatgaa
     4021 tgatatcgaa agagcaaaag tatatacgtg aggcaggagg agcgcacctg atactatgcg
     4081 gataaggaaa tatggggcga gacagcggga agaggattcg tcgacgtaaa aaataatgtg
     4141 gccgacgatc tgtcggccac tcagtacaag acttaacgca cgttggcgca ggtcaggcaa
     4201 aacgcataac ggttttgtgg gtcgttaaac gcggccagct tgtcgctttc acttttaacg
     4261 gtagaggcta ccgaggcaag cggtgcaggt aacatggcct ggaacgcatc tggcaacatt
     4321 gcccggacag aaccagacat gttgtccatc actttgtcga agaaggtcgg ttcatcaacg
     4381 tagtattcca gatgccactg tttcactttt gccagctctg ctgctttggc gaccgcatca
     4441 tcgaaatccc cgagactatc gaccagcccg ttagcttttg catcctgacc ggtccagacg
     4501 tggccctggg cgattttatc aatctgctcc ggcgtcgaat gacgcgcatc agcaaccagc
     4561 gtgataaagc gtttatagcc attctcaatg cttaattgca tcatctgctg cgcttccggc
     4621 ggcagtgccc tggtgataga aacatccgcc agcggtgaag ttgagacacc atcagtatga
     4681 acaccaatcg aatccagact attttctacg gtggtgatca cgccgaagat accgatagaa
     4741 ccggtcaggg tgctggggtt agccacaatg taattagctg gcgtggaaat ccagtaacca
     4801 ccagatgccg ccatgccgcc catcgataca accacaggct tacccgctgc ccgggctgct
     4861 gccagttcag cgcgaatcac ttcagacgcg gtaacgctgc cgcctgggct attaacacgc
     4921 aggacaatcg ctttcacttt cgggtcaagg cgagcgtcgc ggatttgtgc cgcagtggta
     4981 tcaccgccaa cattcccctg agtttcctcg ccatccataa ttgcgccatt agcgaagacg
     5041 acaccgatgc tgtcaccggt atctgccggc gttttcaatg cgtaatcgta ataactgatg
     5101 gcgcgataat ttttatcagt cttactccag ccgaactctt tggttagagc tttttcgatt
     5161 tcggcactcg atgccagtgc atcgaccagc ttgttttcca gtgcatattt cgcggtatcg
     5221 ccaccggttt tggttaaacc ctcaagcaac ccttgcgcgc cagggaatac ctgctgagca
     5281 gggatctgcc ggttagcggc aacagtattc agatagtttt gccacagctc accaatccag
     5341 cggctgtcag cttcgcgggc tgccggtgac atgtcatcac gaataaacgg ttcaacggca
     5401 gatttatacg tacccacgcg gaacacatgg gtggaaactt tcagcttatc cagcaacgat
     5461 ttgtagtaca gaccgttggt agcaaaaccg tgcaaatcca ccacgccttg cggtgacagc
     5521 caaattttat tggcgaaact ggcgagataa tattgcccct ggctgtagtt ctcgccaacg
     5581 gcataaaccg gtttcccgct gtcacgaaac tctttcaggg ctttgccgat gtactgcata
     5641 gacggctggt cgccgcctgc gaagtttttc agatccatca cgataccggt gatattgcgg
     5701 tcgtccttcg cctggcgaat ggtgttgacg atatcaaaca gtgagttttc ctgcagacga
     5761 tcggaactgg caccaagcag ctggcggctt aatttactaa accgctgaga actgtcgggt
     5821 ttatcgacga tcacaccaga aatgtccagc agcagtgcgc cacgactggc cgtttctttc
     5881 gaatcaccac cactgacctg catccagatc cccacaccaa ccagtacgag gaaaataaag
     5941 aacaggttaa gtaccatttc acggacgaaa ttcagcagac gccacgtcca tttaaaaaat
     6001 ccggcaataa atcgccaaag ggttcgcatg tattctccca acttaaggtc acacctgtca
     6061 caggcgcaat agatgtggtt atcgtaatga cacaaccggc acttgtcagc aggaattgcg
     6121 gactgtgctg taacaaaatc cactctcgtg ttaattttgt gaataaatat cacgacagga
     6181 gttaatcaaa tggatgcact cgaactattg atcaatcgcc gtagcgcctc ccgcctggct
     6241 gaacccgcgc caacgggtga acaactgcaa aacatcctgc gtgcgggtat gcgtgcgccg
     6301 gaccataagt ccatgcaacc gtggcatttt tttgtgattg aaggggaagg gcgcgagcgt
     6361 ttcagcgccg tactggaaca gggggcgatt gctgccggta gtgatgacaa agctatcgac
     6421 aaagcccgta atgcgccgtt ccgcgcaccg ctcatcatca cggtggtggc gaaatgcgaa
     6481 gaaaatcata aagtcccgcg atgggaacag gaaatgtctg ccggatgcgc ggtcatggcg
     6541 atgcaaatgg cagccgttgc ccagggattt ggcggcatct ggcgcagtgg cgcattaact
     6601 gaaagtccgg tagtgcgtga agcattcggt tgccgtgagc aggataaaat tgtcggtttt
     6661 ctctacctcg gcacgccgca gttgaaagca tctacgtcga ttaacgtccc ggacccgacg
     6721 ccgtttgtaa cttatttctg ataattgtcg cgaaactgtc tggattgtga gcaaaggcgc
     6781 aggaattcag acactctcac ttatcacttc acggaatgag ggttaccata cccggattgc
     6841 attgacagga gatgtccatg agcgagaact cgattcgttt gacccaatac agccacggag
     6901 ctggttgcgg ctgtaaaatt tccccaaaag tgttggaaac catcctgcac agtgagcagg
     6961 cgaagtttgt tgatccgaat ttgcttgtgg gtaatgagac ccgcgacgat gcggcggtgt
     7021 acgatctggg caatggcacc agcgttatca gtaccaccga cttctttatg ccgatcgttg
     7081 ataatccttt cgattttggc cgcattgcgg cgactaacgc catcagcgat atcttcgcga
     7141 tggggggcaa accgattatg gcgattgcga tcctcggctg gccgattaac aaactttccc
     7201 cggaaattgc ccgcgaagta accgaaggtg gacgctatgc atgccgtcag gcgggtattg
     7261 cgctggctgg cggtcactcc atcgatgcga cggagccgat ttttggtctg gcggtaacgg
     7321 ggatcgtacc gaccgagcgg gtgaagaaaa acagtaccgc acaagccgga tgcaaactgt
     7381 tcctgacgaa accgctgggg atcggcgttc ttaccacggc tgagaaaaaa tcactgctga
     7441 aaccagaaca tcagggacta gcgacggaag tgatgtgccg gatgaacatc gcaggcgcgt
     7501 cctttgccaa catcgaaggc gtaaaagcga tgaccgacgt tacgggtttt ggtctgctgg
     7561 gccatttgag cgaaatgtgt cagggcgcag gtgtgcaggc gcgcgttgac tatgacgcga
     7621 tcccaaaatt gccgggggtt gaagagtaca ttaagttggg cgcagtgcct ggcggcaccg
     7681 aacgtaactt tgccagctac ggtcatctga tgggtgaaat gccgcgtgaa gtgcgcgatt
     7741 tgctgtgcga tccgcaaact tctggcggtt tgctgctggc ggtcatgccg gaagcagaaa
     7801 atgaggtcaa agctacagcc gccgagtttg gcattgaact gacggcaatt ggcgaactgg
     7861 tgccagcgcg cggcggtcgt gccatggttg agattcgtta attcaatgcg gttgtttatt
     7921 gccgaaaaac cgagtctggc gcgcgccatt gctgatgtcc tgcccaaacc gcaccggaaa
     7981 ggcgatggct ttatcgagtg cggtaatggt caggtggtga cctggtgtat cggtcacctg
     8041 cttgagcagg cgcagccaga cgcctacgac agccgctatg cgcgctggaa tcttgcggat
     8101 ttgccgatta tcccggaaaa gtggcaatta cagccccgac cctccgtgac caaacaactt
     8161 aacgtcatca aacgcttcct gcatgaagcc agcgaaatcg ttcacgccgg ggacccggat
     8221 cgtgaagggc aactgctggt ggatgaagtg ctggactatc tgcaactggc accggaaaag
     8281 cgccagcagg tgcagcgttg cttgataaac gacctgaacc cgcaggcggt tgagcgggcg
     8341 ctcgaccgtc ttcggtccaa cagtgagttt gtgccattgt gcgtttctgc gctggcgcga
     8401 gcgcgtgccg actggctgta cggcatcaat atgacccgtg cgtataccat tctcggtcgc
     8461 aatgccggtt atcagggcgt actttccgtg ggacgcgtgc agacacccgt gctcgggctg
     8521 gtggtgcgcc gcgatgaaga gattgaaaac ttcgtggcga aagacttctt tgaagtcaaa
     8581 gcacatatcg tgacacctgc cgatgagcgg tttaccgcta tctggcaacc gagcgaagcg
     8641 tgtgaaccgt accaggatga agaagggcgc ttgttacatc gtccactggc ggagcatgtg
     8701 gttaaccgca ttagtggtca accagcaatt gtcaccagct ataacgataa acgggaatca
     8761 gaatccgcgc cgctgccttt ttcgctttcg gcgttgcaga ttgaagcggc aaaacgcttt
     8821 ggtctgagcg cgcagaacgt gcttgatatc tgccagaagc tgtacgaaac gcacaagcta
     8881 atcacttatc cgcgttccga ttgtcgctat ttgccagaag aacattttgc cggacgccac
     8941 gcggtgatga atgccatcag cgttcatgca ccagatctgt tgctgcagcc agtggtagat
     9001 ccagatatac gcaaccgctg ttgggatgac aaaaaggtcg atgcacacca cgccatcatc
     9061 ccgaccgcac ggagttcagc catcaacctg acggagaacg aagcgaaggt ctataacctg
     9121 attgcccgtc agtacttgat gcagttctgc ccggatgtgg tgttccgcaa gtgtgttatc
     9181 gaactggaca ttgccaaagg caaatttgtc gctaaagcgc gttttcttgc tgaagcaggc
     9241 tggcgcacgc tgttaggcag caaagagcgc gatgaagaaa acgacggtac gccattgcct
     9301 gtggtggcga aaggcgatga gttgctgtgt gaaaaaggtg aagtggtaga gcggcaaacc
     9361 cagccgccgc gccattttac cgatgcaaca ctgctttcgg cgatgaccgg gatcgcgcgc
     9421 tttgtgcagg acaaagatct gaaaaagatc cttcgtgcga ccgatggtct ggggacagaa
     9481 gcaacccgtg ccgggattat tgaactgctg ttcaagcgtg gttttctgac caaaaaaggg
     9541 cgctatatcc actctaccga cgccggaaaa gcgttattcc attcgctgcc agaaatggcg
     9601 acgcgaccgg acatgaccgc gcactgggaa tcggtgctga cgcaaatcag cgaaaagcag
     9661 tgtcgctatc aggactttat gcagccgctg gtggggacgc tatatcagct tattgatcaa
     9721 gccaaacgta cgccggtgcg gcagtttcgc ggcattgtgg ctccgggcag tggtggcagt
     9781 gctgataaga aaaaggctgc accgcgtaaa cgtagtgcga aaaaaagtcc gccagcagat
     9841 gaagtcggaa gcggggcgat agcgtaagcg agtgaatctt tcgtgctatt cgagtc
//
LOCUS       DAETQN010000147         9850 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.148, whole genome shotgun sequence.
ACCESSION   DAETQN010000147 DAETQN010000000
VERSION     DAETQN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9850
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.148"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="K0W68_003728"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="K0W68_003728"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBI3075176.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="K0W68_003729"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="K0W68_003729"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBI3075177.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="K0W68_003730"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="K0W68_003730"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBI3075178.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="K0W68_003731"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="K0W68_003731"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBI3075179.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="K0W68_003732"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="K0W68_003732"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBI3075180.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="K0W68_003733"
     CDS             5058..6191
                     /locus_tag="K0W68_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBI3075181.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="K0W68_003734"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="K0W68_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBI3075182.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="K0W68_003735"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="K0W68_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBI3075183.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="K0W68_003736"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="K0W68_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBI3075184.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="K0W68_003737"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="K0W68_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBI3075185.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="K0W68_003738"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="K0W68_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBI3075186.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="K0W68_003739"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="K0W68_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBI3075187.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..>9850
                     /locus_tag="K0W68_003740"
     CDS             9762..>9850
                     /locus_tag="K0W68_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075188.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPR"
BASE COUNT         2517 a         2504 c         2502 g         2327 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctggtc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc catccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg
//
LOCUS       DAETQN010000148         9688 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.149, whole genome shotgun sequence.
ACCESSION   DAETQN010000148 DAETQN010000000
VERSION     DAETQN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9688
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.149"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..667)
                     /locus_tag="K0W68_003741"
     CDS             complement(<1..667)
                     /locus_tag="K0W68_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBI3075189.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
     gene            complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="K0W68_003742"
     CDS             complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="K0W68_003742"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBI3075190.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5042..5647
                     /gene="azoR"
                     /locus_tag="K0W68_003743"
     CDS             5042..5647
                     /gene="azoR"
                     /locus_tag="K0W68_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBI3075191.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5698..7014)
                     /locus_tag="K0W68_003744"
     CDS             complement(5698..7014)
                     /locus_tag="K0W68_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HBI3075192.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7004..8761)
                     /locus_tag="K0W68_003745"
     CDS             complement(7004..8761)
                     /locus_tag="K0W68_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HBI3075193.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8777..9640)
                     /locus_tag="K0W68_003746"
                     /pseudo
     CDS             complement(8777..9640)
                     /locus_tag="K0W68_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT         2461 a         2635 c         2284 g         2308 t
ORIGIN      
        1 tatcatcgcg taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc
       61 ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa
      121 cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga
      181 acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc
      241 gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg
      301 actggtcttc aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg
      361 cctggatggt cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg
      421 cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa
      481 tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg
      541 caaggatcac gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc
      601 actgtccgat aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa
      661 tgttcatggt tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc
      721 ggagcactct gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata
      781 tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt
      841 ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat
      901 gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc
      961 gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa
     1021 ctaacacgca actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt
     1081 gcaggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc
     1141 atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc
     1201 tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga
     1261 tataccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc
     1321 acccgcccct tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc
     1381 ttcgcaatat ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca
     1441 aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag
     1501 gagatacagt cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc
     1561 ttggctttgt ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc
     1621 agtagacggc gaagaccgtt ccacattacc tgtttttgct ccagcggatt atcaaacagt
     1681 ttgatcgcca cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg
     1741 ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagtccgctc
     1801 tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca
     1861 tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg
     1921 cggaaggtga ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg
     1981 gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc
     2041 ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt
     2101 accggtttcg gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc
     2161 tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc
     2221 acaccgtccg cgtccgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc
     2281 tgatgccaga agttcgggta atccagcttt ctgatttttt ccgccccttc tttgatcaac
     2341 atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta
     2401 tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacagc
     2461 gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg
     2521 gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc
     2581 agagcgtggc gaataaagag ttcacgacat aacgccgaat cgatctggct gtagttgacc
     2641 ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg
     2701 ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg ctgagcaacg
     2761 ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct
     2821 accagttccg ccaccattac ccatttcggc ggctttttga ataaaccaga accggggaag
     2881 atggagaaac gcgcgttacg tgcaccggta tattcctgtt tatcggcatc tttcatgccg
     2941 atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cacgatactc cgccggttcg
     3001 ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta gatatcctgc
     3061 cattcgcgca cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg
     3121 gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag
     3181 tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc
     3241 ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg
     3301 cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc
     3361 gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc
     3421 gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg
     3481 acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag
     3541 gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct
     3601 tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga
     3661 ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg
     3721 tagcggctga tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc
     3781 gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg
     3841 cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag
     3901 ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc
     3961 agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat cgcctgcaac
     4021 tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga
     4081 taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc
     4141 gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct
     4201 ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg
     4261 atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg
     4321 gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg
     4381 atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc
     4441 gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata
     4501 cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc
     4561 tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca
     4621 ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca
     4681 atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttcttc
     4741 acgccgtgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga atccagccgc
     4801 tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtagt
     4861 ctgcctgatt aattttcttt gaaatgatag atatagagta ccacatcacg gctttctatg
     4921 cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt
     4981 gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac
     5041 catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca
     5101 gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt
     5161 tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg
     5221 tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt ccgatgagct
     5281 gattgccgag ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa
     5341 catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg
     5401 ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag
     5461 ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt
     5521 cctcggcttt atcggcatta ctgatgtgaa atttgtcttc gccgaaggga tcgcatacgg
     5581 tccggaaatg gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc
     5641 tgcataaccg gacacgatct tgatgtggac tgtagtccag tccacatccc aatccactta
     5701 cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa tatgcgagcg
     5761 tctggctcga ataaagctaa tcgcttcatc aacggtttta cagtatccgt aacataacag
     5821 ccatgccgcc actaccagcg cactgcgcga taatcccagt gcgcaatgga ccagaacgct
     5881 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct ccccctcttc
     5941 cggaaccacc agatctagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc
     6001 acgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     6061 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     6121 acgcatagat agccacatcc cgatgcgcca gggcaatgtc agccagtaaa cggcgggcgg
     6181 gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     6241 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     6301 catcattagc tccatcaaca caatgcacgc gcacgcgcct acgacatatg gcagtgctat
     6361 tttgattcga cgttgatcag gtttctgata attccaacga cggtcgacgg gcaccatcca
     6421 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccatgt
     6481 cgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     6541 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     6601 agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc
     6661 actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc
     6721 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca
     6781 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg
     6841 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg ggaatatcat ggctattact
     6901 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6961 caacaataac cagccagctc cttgtagcaa tacgttccgg cgttcagtta tcacgacgca
     7021 ccgccatcga aaccgtaaaa atgccccact catcaatccg ttgtgtgcat ttatcaaatc
     7081 cagcatcatg cacgagcaaa tccatctccc cttgcgtacg cacgcgcatt acccacagtt
     7141 taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     7201 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagtgagt
     7261 tttttacctg ctcattttcg ggaaacagct catacagtcc agagacaatc gccagcgtag
     7321 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     7381 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     7441 tgtaatcacg taataaaata tcgcttacgg caggctcgtt tgccagtgca tccagtacat
     7501 agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg acgtggagat
     7561 cggcaacggc ttgtttaatc agtatttgta aatgagtttt gcgctggcga ataccgcgcc
     7621 agccaacact gttcaggtaa attttgtcga ttaagcgccc gaatgcgtta ctgccttgcg
     7681 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccagtg ctcattccga
     7741 gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc tttacgcata aagcgatacg
     7801 ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gaggcgccag cgatccgctg
     7861 atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta gcatataaac
     7921 gacgaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaacgtgt
     7981 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     8041 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     8101 ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttatag agatcgagca
     8161 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     8221 gccgatcgtg ggtcaaatag cgtcttttca cataggaatt aataaaaaac agaccacgca
     8281 gacgatgcca tagcgtcagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     8341 ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     8401 ccagcactgc gccgacgctt tgcgcgatca caaccacctc ttccagtcca acctggctgt
     8461 cgctggcagc aaaacggaca aattcatcga catcctgcac tgaacgcgca agagatgggc
     8521 tataaccacg cggccccgaa gtttttccat gcccccgggc atcccatgca taaaaagcag
     8581 tatctggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatggccac
     8641 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8701 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agagatgcgt gaattttcca
     8761 ttttctctat tttccttcag taacagcagt aacgaataaa ataaaaaaat accggagcgg
     8821 tgaaaattaa tgagtcaatc ctgtcaagga ttccgccatg tccaggtaat agtttcccgc
     8881 tatctttaac accaacatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     8941 taataccaat taacaaaccc gctaatattg cctgtaatgt atttagcggt gtcagtaacg
     9001 gtccaatgat catcgacgct atcatggtgg tgattacgcc gccgaccaga ccttccagcg
     9061 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     9121 actgtgcaat atcgtttgac gcggttaatg ccaaaagaaa taacaccagt aaggccccgc
     9181 cctggatatt catggatggc aacattaata actaggcggc atgactcaag gcgaaaacgg
     9241 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gggtcaccca
     9301 caaaaactcg ccaggcggct aatatcagaa acccagccaa aggtataaat aacagaaaca
     9361 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccacc
     9421 gcggaaaatg tacggatata agcatacaat attctttcaa tgccagaaaa cttatcagcg
     9481 caaaaaacgc caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     9541 tgccccacca ggtacgaata cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc
     9601 atagcagaaa acggtttatc agcgttgcta aaattaacaa cgcgaaaagt gaggtgtact
     9661 ggcaatagcg gacactacca tttgttct
//
LOCUS       DAETQN010000149         9622 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.150, whole genome shotgun sequence.
ACCESSION   DAETQN010000149 DAETQN010000000
VERSION     DAETQN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.150"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            224..2200
                     /locus_tag="K0W68_003747"
                     /pseudo
     CDS             224..2200
                     /locus_tag="K0W68_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2193..3329
                     /locus_tag="K0W68_003748"
     CDS             2193..3329
                     /locus_tag="K0W68_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBI3075194.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3326..5326
                     /locus_tag="K0W68_003749"
     CDS             3326..5326
                     /locus_tag="K0W68_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HBI3075195.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5448..5909
                     /locus_tag="K0W68_003750"
     CDS             5448..5909
                     /locus_tag="K0W68_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBI3075196.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5950..6420)
                     /locus_tag="K0W68_003751"
     CDS             complement(5950..6420)
                     /locus_tag="K0W68_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBI3075197.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6467..7186)
                     /gene="yehT"
                     /locus_tag="K0W68_003752"
     CDS             complement(6467..7186)
                     /gene="yehT"
                     /locus_tag="K0W68_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBI3075198.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7183..8868)
                     /gene="btsS"
                     /locus_tag="K0W68_003753"
     CDS             complement(7183..8868)
                     /gene="btsS"
                     /locus_tag="K0W68_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBI3075199.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9090..9155
                     /locus_tag="K0W68_003754"
                     /pseudo
     CDS             9090..9155
                     /locus_tag="K0W68_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            9383..>9622
                     /locus_tag="K0W68_003755"
     CDS             9383..>9622
                     /locus_tag="K0W68_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBI3075200.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWF"
BASE COUNT         2354 a         2531 c         2469 g         2268 t
ORIGIN      
        1 gcggccacga tacgtattga ttgaaggccc ggctgatttt aatgaccggg tagacgaact
       61 gtttttagcc caccagcttc cggtagctat ttacagttat tgccagtatc aggacggtgc
      121 agcccccggg cgtggtgcct ggacgccatt tgctgaattt tcgccggagt ggcaggcgct
      181 acaagccgca cgtcgtattc aggcacaaac ttacttcatc gatttgcctt gctgggcgca
      241 gagtgaagaa gtggacgatt cgcctgatac gcaagaggaa agccaggcct tgctgctgcg
      301 tgccacccgc atggataaca gcgacaccct gtgggatcac ttgttcgaag atgaaagcca
      361 gcaaactgca ttaccctctg cactggcgca ctattttgcc caactgcggg gcgattcccc
      421 cggcgatgca ctcaatcgtc agcgcgaagc ctttatggcc cgctggattg gatgggcgat
      481 gcagcaaaac aatggcgacg tgttagtcgt ctgcggtggc tggcacgctc ctgcactggc
      541 aaaaatgtga cgcgaatgcc cgcaggacat taacacgccg gaattgccct cgctggcaga
      601 tgccgttaca ggttgttatc tcacccccta cagtgaaaag cgccttgatg tgctggcagg
      661 atacctttcc ggtatgcctg ccccggtctg gcaaagctgg tactggcagt ggggcttaca
      721 gcaggcggga gaacaactgc taaaaacggt tcttacccgt ttgcgccagc acaaattgcc
      781 tgcttcgacc gcggatatgg ctgccgctca tctgcatgcg atggcgctgg cacagttgcg
      841 cggacataaa ctaccgttac gcactgactg gctggatgcc atagcaggct cgctgattaa
      901 agaagccctg aacgcgccgt tgccgtggag ctatcgcgga gttattcatc ccgacaccga
      961 tccgattctg ctaacgttga tagacacatt agcgggtgac ggattcggta aacttgcccc
     1021 ttctacgcca caaccgcctc tgccaaaaga tgtcacctgc gaactggaac gtaccgcaat
     1081 ctctcttccg gcggagctta ccttaaatcg ctttaacccc aatgggctag cgcaaagtca
     1141 ggtgttacat cggctggcaa tactggagat cccagggatt gtacgccagc agggaagcac
     1201 actgacactt gcaggcaacg gtgaagaacg ctggaaatta acccgcccgc ttagccagca
     1261 tgcggcattg attgaggccg cctgctttgg tgccacactc caggaagccg cacgccataa
     1321 attagaagcc gatatgctgg acgcgggtgg aatcggcagt atcaccacat gtcttagcca
     1381 ggcggcgtta gcgggtctgg cgtccttcag tcaacaatta ctggagcaac tcacattatt
     1441 aatcgcccag gaaaatcaat ttgccgaaat gggccaggcg ctggaagtgc tatatgcctt
     1501 atggcggctg gatgaaatta gcggtatgca aggcgcgcag atattacaaa cgacgttatg
     1561 cgcggctatc gatcgcacgc tgtggctgtg tgaatctaac ggcagaccag atgaaaagga
     1621 gtttcacgct cacctgcata gctggcaggc gctttgccat atcctgcgcg atctacatag
     1681 cggcgttaat ttacccggcg tttttctttc tgcggcggta gccttactgg agcgccgcag
     1741 tcaggcgatt catgtcccgg cgctggatcg cggcgcggct cttggcgcac tgatgcgtct
     1801 ggaacatccc aacgccagtg ccgaagcggc gctgacgatg ctggcgcagt tatccccggc
     1861 acaatccggc gaggcgctgc acggtttgct ggcgctcgcc cgtcatcaac tggcctgcca
     1921 accagcattt atcgccggtt tcagtagtca tttaaatcaa ctgagtgacg acgattttat
     1981 taacgccctg cccgatttgc gcgcggcgat ggcctggcta ccgccacgag aacgcgggac
     2041 actggcgcat caggtgcttg agcattacca gcttgcgcag cttcctgttt cagcgctgca
     2101 aatgccgttg cattgtcctc cgcaagccat tgcacatcat caacaactcg aacagcaggc
     2161 actggcatcg ctgcaaaact ggggagtttt ccatgtctga actgaacgat cttctgacca
     2221 cccgtgagct acaacgctgg cgattaattc ttggcgaagc ggcagaaacg acgctttgtg
     2281 ggctggatga caacgcccgg cagatagacc acgcgctgga atggctgtat gggcgcgatc
     2341 ctgaacggct ccagcgtggt gaacgctccg gtggattagg tggctcaaat ctcaccactc
     2401 ctgagtggat caacagtatt cacacgctgt ttccgcaaca ggtgattgag cggctggaaa
     2461 gcgatgccgt actgcgctac ggcattgaag atgtggtgac gaatctcgac atgctggaac
     2521 gtatgcagcc ttctgaaagc ctgctacgcg ccgttttgca caccaaacat ctgatgaatc
     2581 ccgaagtact ggctgccgcc cgccggatag tgcgccaggt tgttgaagaa attatggctc
     2641 gactggcaaa ggaagttcgt caggcttttt ctggtgtccg cgatcgccgt cgccgctcat
     2701 ttattccact ggcgcgaaac tttgatttca aaagtactct gcgcgctaac ctgcaacact
     2761 ggcacccgca acacggcaag ttgtatatcg aatccccccg ctttaacagc cgcattaaac
     2821 gccaaagcga acaatggcaa ctggtcttac tggttgatca aagcggatcg atggtggact
     2881 cggtgatcca ctctgcggtg atggcggcct gtttgtggca gttacccggc attcgtaccc
     2941 atctggtggc gtttgacaca agcgtggttg atctcacggc agacgttgcc gatccggtag
     3001 agttattaat gaaagtacag ttgggcggcg ggaccaatat cgccagtgcc gtggagtatg
     3061 gtcggcaact tattgaacaa ccagcaaaaa gcgtcattat cctcgtgagc gatttttacg
     3121 aagggggttc atcatcattg ctgacgcatc aggtgaaaaa gtgtgtccag agcggcatca
     3181 aagtgctggg actggctgcg ctcgatagca ccgcaacacc ttgctatgac cgcgatacgg
     3241 cccaggcgct ggttaatgtc ggcgcacaaa tagccgccat gacgccgggc gagctggcat
     3301 catggcttgc ggagaatctt cagtcatgaa ttcactacgt ccggaattat tagaactgac
     3361 accgcaggcc ctgacggcgt taagcaatgc cggttttgtt aagcgcagtc ttaaggaact
     3421 ggaaaatggc aacgtcccgg agatcagcca tgagaacgac gctttaatcg ccaccttcag
     3481 tgacggtgtc cgtacccagc tggcgaacgg ccaggcactg aaagaggctc agtgcagttg
     3541 cggggccaac ggtatgtgcc gtcatcgcgt gatgctggtg ttaagttatc aacgactttg
     3601 tgccaccact cagtctacgg aaaaagaaga agagtgggat ccggcaatct ggctggaaga
     3661 actggctacc cttcccgatg ctacccgcaa acgcgcacag gcgctggtcg ctaaaggcat
     3721 caccattgag ttgttctgtg cgccgggtga aattccctct gcccgcttac cgatgagcga
     3781 tatgcgtttt tattcccgca gcagtattcg tttcgcccgt tgtgattgta ttgaaagcac
     3841 actttgcgaa catgtcgtac tggcagtaca ggcctttgtc caggcaaaag cgcagcaagc
     3901 ggaattgact catttaatct ggcagatgcg cagcgaacac gtcacatcat ctgacgatcc
     3961 gtttgccagc gaagaaggca aaacgtgtcg tcaatatgtt caacaactaa gccaggcatt
     4021 atggctgagc ggcatcagcc agccgcttat tcactacgag gccgctttca gccgcgcgca
     4081 gcaagcagcg gaacgctgca gctggcgatg ggtgagtgaa tcgctacggc aactgcgcgc
     4141 cagcgttgat gccttccacg cccgcgccag ccactatcat gccggagaat gcttacgtca
     4201 gcttgcggca ttaaacagtc gattaaattg cgcacaagag atggcccggc gcgacagtat
     4261 tggtgaagtt cctcctgtgc cgtggcgcac ggtcgttggc tctggcattg ccggagaagc
     4321 aaagcttgat catctgcggc tggtgtcttt aggtatgcgt tgctggcagg atattgagca
     4381 ttatggttta cgcatctggt ttaccgatcc cgacaccggc agtattttgc acctttcgcg
     4441 cagttggccg cgaagtgaac aggaaaactc accggcagct acgcgtcggc tgtttagttt
     4501 tcaggctggc gcactagcgg gcgggcaaat tgtttcacaa gcagcaaaac gcagtgccga
     4561 tggcgagctg ctgttagcta cccgcaaccg cttaagcagc gttgtgccgc tgtcgcctga
     4621 tgcctggcaa atgttgagcg cgccgttacg ccagccgggc attgtggctt tgcgggaata
     4681 tttacgccag cgtccccccg cctgcatacg gcctcttaat caggtcgata acttatttat
     4741 tctgccggtc gctgagtgta tttcgctcgg ttgggatagc agccgccaga cgctggatgc
     4801 gcaggtaatc agcggcgaag gtgaagataa tctcctgacg ttatcccttc cggcatctgc
     4861 ctgttctcct tttgccgttg aacgcatggc ggcgcttttg caacaaacag acgaccccgt
     4921 gagtctggtt tctggctttg tcagctttgt agaggggcaa ttgacactgg aaccacgggt
     4981 gatgatgaca aaaacccgcg cctgggcgct ggacgcagaa actgcgcctg tggcaccgct
     5041 accttctgcc agcgttttgc ctgtgccgtc taccgctcat cagttgctga tgcgctgcca
     5101 ggcgttactt attcaactgc tccataacgg ctggcgctat caggaacaga gcgctattag
     5161 tcaggcagag ttgctggcga atgacctctc cgcggtcggt ttttatcggc tggcacatgt
     5221 gttgggacaa tttcgtaata cagaaagcga ggcacgggta gaagcaatga ataacggtgt
     5281 tttactttgc gaacaattat tccccatgct tcagcaacaa ggatgaaata gtgcttacta
     5341 agagttctac tccagttccg gactgctcac gccacggtat taggcatatc ctatatagcc
     5401 cctggtgaga gtcaccagtt ccttgattaa ataaaatgga gttttacatg aaggctttca
     5461 ataagctgtt ttccctcgtt gttgcatctg ttctggtttt ctctcttgct ggctgcggtg
     5521 acaaagaaga atcgaagaaa ttcagcgcca atctgaacgg cactgaaatt gccattacct
     5581 atgtctacaa aggtgacaag gtgcttaagc aatcttctga aaccaaaatt caatttgcct
     5641 ccattggtgc aaccaccaaa gaagatgctg ccaagacact tgagccgtta agcgccaaat
     5701 acaaaaacat cgcgggtgtt gaagaaaaat taacctatac cgatacctac gcgcaggaaa
     5761 acgtgactat cgatatggaa aaagtggatt ttaaagccct gcagggtatt tcaggaatca
     5821 acgtttctgc tgaagatgcc aaaaaaggta tcactatggc gcaaatggaa ctggtgatga
     5881 aagccgctgg ttttaaagaa gtgaaataat ctgtcggcgg ccatgtttcg catggccgcc
     5941 atacccgtct tagcttttct tcacatgctg gcgcgctgcc agtccacgca gaaaataacg
     6001 taaaaattga tcgccgcatt cgcggaagtt tttatgatcc ggtgcgcgca tcatcgctgt
     6061 aatttccggc atcgaaacgc ggaactgctg ttcggtgagg atcgccagaa tgtcatcggt
     6121 tttcagcgaa aacgcgatgc gtaatttttt cagcacgatg ttgttattaa tgcgacgttc
     6181 cggctccagt gccggagcag actcatcctt gccgcgtttt tcataaatca ggccattgag
     6241 gaatgacgac aaaacaatgt ccggacaacg ctgaaaaccc tcttcgtctt ctttacgtag
     6301 ccagacggcg atctgttccg cggtggcttc gacattaccc agcgccagaa tacgcaccag
     6361 gtcattatta ttggctttca aaatgtagcg cacgctgcgc agaatatcgt tacttagcat
     6421 gaggccttca ggtgttaatg aggcaaaaag ccattttagc agtcttttac aggccaatcg
     6481 cctcttttaa gcttttcaga taacggcggc tgaccggcac ggttaagcca ttacgcaaaa
     6541 tcaactcggc ctggccgtta tcttccagac gaatctcctg taaatgcgcg aggttaacca
     6601 gatactgacg atggcagcgc agtagtggtg tacgactttc cagggtacgt aatgtcaatt
     6661 cggtaaagcc ctctttccct tcgtggctgg taacgtagac accgctcatc cgactgctga
     6721 caaatgccac atctttcatt tgcagcaaat aaatccgact atgccccgta caagggataa
     6781 atttcagcgc ctgttgattt tccggtaaca gcgaaacatc ctgcttgctg cgctcctgac
     6841 gcaatcgcgc cagcgttttc tccagtcgcg cttcatcaat tggcttcagc agataatcaa
     6901 aggcatgttc ttcaaaggct ttaatagcgt attcgtcaaa cgcagtgaga aaaacaatat
     6961 acgggcgatg ttccgggtca agcatcccca ccatttccag accactgatg cgcggcatct
     7021 ggatatcgag aaacagcaca tccgggcgca gtttatgcac cgcgccgatc ccttccacgg
     7081 cgtttgaaca ctctccaacg atttcaatat cgctctgctc ctgcaaaaat acacgcaggt
     7141 tctcccgtgc taacggttca tcatcgacaa ttaagacttt aatcatgcct cgtccctcca
     7201 tggtagtcgt aacgttattc gggtgtaact atcaggctca caggcgacgc ttattccata
     7261 gtcatcgcca aaccgttcac gtaaacgctt atccaccaga ttcatcccca gcccactggc
     7321 attggttacc ggttgataca aaccggcatt gtcttcgatc tccagcatca aatgttgccc
     7381 ctcacgtcgg gcgctgattg ccactcgccc tgtatccagc agttgtgatg tcccatgttt
     7441 aatggcgttt tccactatcg gttgcagggt aaacgcgggc aattgctgct gggataattc
     7501 ttgcggaata gcaatgttga cctgcaaccg cgactggaag cgcgcctttt caatttgcag
     7561 ataagcgttc acatgttcaa tttcgtcggc gagagtaaca aactccgaag gccgctttaa
     7621 gtttttgcgg aaaaaagtgg aaagatactg caccagctgg ctggcctgtt cgctgtcgcg
     7681 gcggatcacc gctttaatgg tgttaagcgc attaaacaaa aaatgaggat tcacctgggc
     7741 gtgaagcagt ttgatttctg actgggtgag catcgctttt tgccgctcat attgcccggc
     7801 aagaatctgc gccgaaagca gttgcgcaat cccctcgccc agcgtgcggt ttattgaact
     7861 gaataaacgg tttttggctt catacaattt gatggtgccc atcacgcgct gattttcgcc
     7921 acgcaacgga attaccagcg tcgaccccag tttgcattgc ggatgcaaag agcaacgata
     7981 aggtacttcg ttgccatcag cgtagaccac ttcaccggtt tcaatcgctt ttaaggtgta
     8041 aggcgaagaa atcggtttgc cgggtaaatg gtggtcgtca ccaattccgg taaaggccag
     8101 caatttctct cgatcggtaa tcgcgactgc accaatatcc agctcctgat acagcacctg
     8161 agccactttc atgctgttca cttcgttaaa cccctgtcgc aaaatgcctt ccgtcgaggc
     8221 tgccactttc agcgcagtgg cagaaaaagc cgaagtgtat ttttcaaaca tcgcgcgttt
     8281 atcgagcaat atacgcataa acagcgccgc gccgacggta ttggtgacca tcattggcgc
     8341 agcaatatta ctcaccagac gcaccgcatc ttcataaggt cgggcgatcg ccaggatgat
     8401 cagcatttgc accatttcag cgacgaacgt gacggcaccg gcggtaatgg ggttaaagac
     8461 tttatcagtg cgcccgcgac ggatcaggat gctgtgtacc aggccgccga gcaatccttc
     8521 aacgatggtt gagatcatgc aacttagcgc ggtcatgccc cccatcgaat atcgatgtaa
     8581 gccgccggtc agaccaacca gcccaccgac gaccggaccg ccgagtaagc cgcccattac
     8641 cgcgcctatc gcacgggtat tggcaataga atcgtcaatg tgcaacccaa accaggtgcc
     8701 catgatgcag aagatggaaa agacgatgta gcagagaaat ttatgcggca gacgaaccgt
     8761 gacctgcatt aacggtatga ataatggcgt tttactcatt aaccacgcaa tgactaaaaa
     8821 aacgcacatc tgctgaagca gcagcaacac cagattaaaa tcgtacatac ccgcaaacca
     8881 cacttccctt taaaacgcgt aacatacatt gcctgcgttt aactttcttt gaactcttgc
     8941 agaaaaatga gaattcgtga gtacgatcac tcaaaatcgc ctggcaaaaa taaaatcacc
     9001 ctatagatgc acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa
     9061 accgggacct cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt
     9121 tgctttgtga tattaaccct gtcacgttac gcgcgtgacg caacattttt aaaaatcgac
     9181 gaagatcgtc caggagcgcc gtttttcaag ggttggatag atgattcgtt taccatgtcg
     9241 acgaacttcg acgaatttcg ccgataaaaa gtgtcccata catgccccat tgatgcccca
     9301 taaacaatat caggaattga cactggttat ccatacagat aaaaataaca cctgtacata
     9361 accacagtta caggagggtg ttatgcgtgt tgagatctgt atcgctaaag aaaaaatcac
     9421 taaaatgcca aacggtgctg tggatgcgtt aaaggaagaa ttaacccgac gcatcagtaa
     9481 acgttatgac gatgtagagg tgatcgtaaa agccaccagc aacgatggcc tttctgttac
     9541 acgcaccgca gataaggatt ctgcaaaaac atttgttcag gagactctga aagatacctg
     9601 ggagtctgct gacgagtggt tt
//
LOCUS       DAETQN010000150         9619 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.151, whole genome shotgun sequence.
ACCESSION   DAETQN010000150 DAETQN010000000
VERSION     DAETQN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.151"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..203)
                     /locus_tag="K0W68_003756"
     CDS             complement(<1..203)
                     /locus_tag="K0W68_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HBI3075201.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPAGNDSNLLIVFYVQI"
     gene            complement(203..1129)
                     /gene="glsB"
                     /locus_tag="K0W68_003757"
     CDS             complement(203..1129)
                     /gene="glsB"
                     /locus_tag="K0W68_003757"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBI3075202.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(1193..2581)
                     /gene="sad"
                     /locus_tag="K0W68_003758"
     CDS             complement(1193..2581)
                     /gene="sad"
                     /locus_tag="K0W68_003758"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HBI3075203.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2682..3563
                     /locus_tag="K0W68_003759"
     CDS             2682..3563
                     /locus_tag="K0W68_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3075204.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            3640..4755
                     /gene="yneK"
                     /locus_tag="K0W68_003760"
     CDS             3640..4755
                     /gene="yneK"
                     /locus_tag="K0W68_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HBI3075205.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            4905..6095
                     /gene="ydeA"
                     /locus_tag="K0W68_003761"
     CDS             4905..6095
                     /gene="ydeA"
                     /locus_tag="K0W68_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBI3075206.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(6120..6785)
                     /gene="marC"
                     /locus_tag="K0W68_003762"
     CDS             complement(6120..6785)
                     /gene="marC"
                     /locus_tag="K0W68_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBI3075207.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            6997..7431
                     /gene="marR"
                     /locus_tag="K0W68_003763"
     CDS             6997..7431
                     /gene="marR"
                     /locus_tag="K0W68_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBI3075208.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            7451..7834
                     /gene="marA"
                     /locus_tag="K0W68_003764"
     CDS             7451..7834
                     /gene="marA"
                     /locus_tag="K0W68_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBI3075209.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            7866..8084
                     /gene="marB"
                     /locus_tag="K0W68_003765"
     CDS             7866..8084
                     /gene="marB"
                     /locus_tag="K0W68_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBI3075210.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(8141..9580)
                     /locus_tag="K0W68_003766"
     CDS             complement(8141..9580)
                     /locus_tag="K0W68_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBI3075211.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         2485 a         2465 c         2167 g         2502 t
ORIGIN      
        1 attatctgaa cataaaacac tatcaataag ttggagtcat tacctgccgg agcaagacgt
       61 ttagcgacaa aatccgccgc atgttgttcg atgactggcg cgcctttctc caggcaatac
      121 tgacgctctt tcatgcccag acgaaagcga gagcgaaatt ttgaacggga taaacgcgcg
      181 aagagtggat cgagagactg cattaataaa ccgaacgtcc taactgtttc gtcaattgtt
      241 caaggacggc gatacccgca agcgagttac ctgcatcatc cagttccgga ctccagactg
      301 cgatggccat ttcatgcgga acaatcgcca caataccgcc accaacgcca gatttcgccg
      361 gtagtcccac ccgccaggca aactcccccg cgttctggta cataccactg gtcgccatca
      421 gcgcgttaat ttgccgcgcc tgcattggcg tcactacagg ttcatcaata tgaatagctt
      481 tcccctgatt agccagaaag acaaacgtcc gagccagctc tacacagctc attttcagag
      541 cgcagtaatg aaagtagttt tgcagaacgg ttgtcacgtc atgatggaaa ttgccaaacg
      601 acttcatcag ccaggcgata gccgcatttc gcgcggaatg ttcaaattcg gaacgcgcta
      661 ccaccgtatc gtatgaaata tcagacacac cgcttaagcc gcgcacgact tccagcatac
      721 gttgccgtgg tgcgcttaat cgcccttgca acatatcgca gaccaccagc gcaccagcat
      781 taataaacgg attacgcgga ataccctgtt ccatttccag ttgcactaaa gaattgaacg
      841 gtgatccaga cggatctttg ccgacgcgtt gccagatttc ctcttcggag taatgacgca
      901 tggcgacaac gagactcagc actttggaaa tagactgaat ggaaaaacgt tcttgcgcgt
      961 ctccggcctg aaaaagctgt ccgtcaacgg tacagatagc aatgcccaat cgggaaccgt
     1021 ctactgtagc cagcgccgga atataatccg cgactttacc ctgaccaatg agcggccgca
     1081 cttgccgcaa gatgttttct aaaattgcat tatccatggc gactgccact ttctactcct
     1141 ggaccgcagg tctgaaaaga cctgcgagta tatcagagct gaatatgtcg cgtcagatcc
     1201 ggtctttcca caccgtctgg atattacaga attcgtgtaa gccgaaatgg gaaagctcac
     1261 gaccaaagcc actctttttc acgccaccaa aggccactcg cgcgtcgctg gcacaataac
     1321 cattgataaa caccccaccg cattccagac gtgccgccat ctgtctggcc tgtgtttcgt
     1381 cggtggtaaa aatggtcgct gaaaggccga actcactatc attagccagt tccagcgcat
     1441 gttctgcatc tttcgcaacg gtgattgccg caacggggcc aaacatttct tcccgaaacg
     1501 cggtcatttc tggggtaaca ttcgccagaa ccgttggcgg atagtagtta cctgccccag
     1561 ccatcttttc cccgcccagt aacaaacgcg caccctgcgc cagggttttc tctacctgat
     1621 gatgcagctc atcacgtaaa tcaaaacgag ccattggtcc gagagtgttc tcttcgtcac
     1681 ggggatcgcc cattttcaag gctgccgcag ctgccacaaa acgttcggta aatgcagaag
     1741 caattccctc ttcgataata aagcgttttg ccgctgcaca tacctgtccg gtattctgat
     1801 aacgtccggc taccgccgct ttaaccgcca gttccagatc ggcatcgtta agcacaataa
     1861 acggatccga accgcccagt tccagtacgc attttttcag tgccgctcca gcctgtgcgc
     1921 caatagccgc tccagcacga acacttccgg tcaccgtgac agcagcaatg cgcgagtctt
     1981 taatcatctg actgacaccg tcgttgtcgg cattcagcca gccatatacg ccttgcggga
     2041 ttcccgcatc tttaaacacc tgggcaatga gctgagcaca tcccatcaca ttcggcgcat
     2101 gtttaagtaa gtagccgtta cctgcaagaa tgatgggaac agcgccacgc atcacctgcc
     2161 ataacggaaa attccacggc ataatcgcca gaatcgtccc caacggtcga tactcaataa
     2221 ccgcctgctg attttccacc agtgtaggtt ccgccttcag cattgccgga ccatgttctg
     2281 cataccagtc acacaaattc gccgatttcg ccacttcagc gcgcgcctgg ttgattggtt
     2341 tgcccatttc gcgggtgatc atttgcgcca tttcttcgct acgggcgcgc agagccttac
     2401 cgatatcacg cattttttca gcacgataat ctatatttgt ctcgcgccag tcgcgaaagc
     2461 ctgctgccgc cagctcaagt gcgttttcga tatcgttagc gccagcccac ggcagcacag
     2521 aaagttgttc acccgtggca ggatttattg aaattgcatg agttgccgga gtaatggtca
     2581 tcggggtatc tcctttatga gtcatggtat gaagatacgc agatttactc ttgctttaaa
     2641 atgaataata ttaagccact tattcacgaa tcgagaatgt tatggatctg acccaactgg
     2701 agatgttcaa cgcggttgcc gaagctggca gcattaccca ggctgcagca aaagtgcatc
     2761 gcgtaccgtc gaatctcact acccgtctac gccagctgga aacagaactg ggggtagatc
     2821 tgtttattcg cgagaatcag cgtttacgtc tctctcctgc cgggcataac tttttacgct
     2881 atagccaaca aattctcgcg ttagtggatg aagcgcggag cgttgtcgct ggcgatgagc
     2941 cgcaaggttt attttctctt ggttcgctgg aaagcaccgc agcagtgcgc attccggcca
     3001 cgctggcgga attcaaccat cgttatccca aaattcagtt ttcactttcc accggccctt
     3061 ccggcactat gctggaagga gtgctggagg gaaaactgaa tgcggcattt attgatgggc
     3121 ccattaacca tactgccatt gacgggatac cggtataccg cgaggagctg atgatcgtca
     3181 cgccacaagg tcatgcgcca gtaatccgcg ccagtcaggt taacggcagt aacatttatg
     3241 ccttccgcac caattgttcg tatcgtcgcc acttcgagag ctggtttcat gctgacggtg
     3301 ccgctccggg aactattcac gagatggagt cgtatcacgg gatgttggcc tgtgtggtcg
     3361 caggagcagg cattgcgctt attccacgct ctatgctgga aagtatgccg gggcatcacc
     3421 aggttgaagc gtggccgtta gctgagcaat ggcgttggtt aacaacctgg ctggtctggc
     3481 gtcgcggtgc gaaaacgcgt ccgcttgagg catttattca actactggat gtgtctgact
     3541 cggcaaaaca gagttaccaa tgaactattt tgataattct tgcggctaat atgctctata
     3601 tagtgatgtt ccgatgactt atgactatat ggggcaaata tggttacgcc agtaagcatc
     3661 agcaattaca tatctcttcc tgatgatttt cccgttcgta atattgcacc acaggtaaaa
     3721 gaagttttaa aggactttat tgatgcgcta agtacaataa tatgtgatga agagtggcgc
     3781 acgtctttaa acatcaactc agccacaaaa aagatattta ataaccttga taacttatca
     3841 tatattcaga ggacttcttt ccggggtaac gacacgctat acaatgaaaa ggttcagttt
     3901 aaactcactt atcccgtcaa gaatggaaga cacaaagaaa atattgaatt tcaggtagta
     3961 ataaatttaa gccctattta tttagataat tttcgccatg atggagaaat taatattttt
     4021 tgcgctccca acccaaagcc tgtcactatg gggcgcgtat ttcagaccgg cgtcgagcgt
     4081 gtgctctttc tgtttctgaa tgattttatt gaacaatttc caatgttcaa ccctggcgtt
     4141 ccgatcaaaa tagcgcatac accacatatt gaacccctgc ctccggatca ccataccgct
     4201 gcagattact tacgccagtt tgatttgctt gtcctgaatt ttatctctcg cggtaatttt
     4261 gtcatactcc cccgattatg gaataactct gaggttcaca gatggttcgt caataaagat
     4321 cctaacttga tcaccgccat tctcgacata acggacagcg aattaaaaga ggatttattg
     4381 caaagcctga tggattcatt aggttctaac aaacatgtac tacccgaagt ctgcatctgc
     4441 tttttattcc ttttagcaga acaagaatct cctcattttc aggacttgtt tttatttttc
     4501 gccaatatgt tattgcacta tcaccaattt atgaatccca atgaaagtga tttgaatgac
     4561 gtgttgatgc cagcatcatt aagtgatgat aaaattatca agcatatggc gcgcaggacc
     4621 ctcgaactgt ttgtaaaaaa tgaaacaccg ccaaaagtta ctcacgaaga cctggtgaaa
     4681 aacaggcctc gctcccctgt cagaccacct atacccgcaa cctccaaaac gccagacctc
     4741 cctgaacgtc attaaaccgt gatgttaccg actctctgac gcggaacaga atcagcgtca
     4801 gagaaacgga aaacgcgatc cagatcacaa atacattgta ttcacatcat taaccgtttt
     4861 aagatcattt catcactttt tcgcaactca cccgataatc tgttatgaca acaaacactg
     4921 tttcccgcaa agtggcgtgg ctacgggtcg ttacgctggc agtcgccgcc ttcatcttca
     4981 acaccaccga atttgtccct gttggcctgc tctctgacat tgcgcaaagt tttcacatgc
     5041 aaaccgctca ggtcggcatc atgttgacca tttacgcatg ggtagtagcg ctaatgtcat
     5101 tgccttttat gttaatgacc agtcaggttg aacggcgcaa attactgatc tgcctgtttg
     5161 tggtgtttat tgccagccac gtactgtcgt ttttatcgtg gagctttacc gttctggtga
     5221 tcagtcgcat tggtgtggct tttgcacatg cgattttctg gtcgattacg gcgtctctgg
     5281 cgatccgtat ggctccggcc gggaagcggg cacaggcctt gagtttaatt gccaccggta
     5341 cagcactggc gatggtctta ggtttacctc tcgggcgcat tgtggggcag tatttcggtt
     5401 ggcgaatgac cttcttcgcg attggtattg gggcgcttat cacccttttg tgcctgatta
     5461 agttacttcc cttactgccc agtgagcatt ccggttcatt gaaaagcctc ccgctattgt
     5521 tccgccgccc ggcattgatg agcatttatt tgttaactgt ggtggttgtc accgcccatt
     5581 acacggcata cagctatatc gagccttttg tgcaaaacat tgcgggattc agcgccaact
     5641 ttgccacggc attactgtta ttactcggtg gtgcgggcat tattggcagc gtgattttcg
     5701 gtaaactggg taatcagtat gcgtctgcgt tggtgagtac ggcgattgcg ctgttgctgg
     5761 tgtgtctggc actgctgcta cctgcggcga acagtgaaat acacctcggg gtgctgagta
     5821 ttttctgggg gatcgcgatg atgatcatcg ggcttggtat gcaggttaaa gtgctggcgc
     5881 tggcaccaga tgctaccgac gtcgcgatgg cgctattctc cggcatattt aatattggaa
     5941 tcggggcggg tgcgttggta ggtaatcagg tgagtttgca ctggtcaatg tcgatgattg
     6001 gttatgtggg cgcggtgcct gcttttgccg cgttaatatg gtcaatcatt atatttcgcc
     6061 gctggccagt gacactcgaa gaacagacgc aatagttgaa aggcccattc gggccttttt
     6121 taatggtacg ttttaatgat ttccaggatg ccgttaataa taaactgcac acccatacat
     6181 accagcagga atcccatcag acgggagatc gcttcaatgc cacccttgcc caccagccgc
     6241 ataattgcgc cggagctgcg taggcttccc cacaaaataa ccgccaccag gaaaaagatc
     6301 agcggcggcg caaccatcag tacccaatca gcgaaggttg aactctgacg cactgtggac
     6361 gccgagctaa taatcatcgc tatggttccc ggaccagcgg tacttggcat tgctagcggc
     6421 acaaaggcaa tattggcact gggttcatct tccagctctt ccgacttgct tttcgcctcc
     6481 ggtgaatcaa tcgctttctg ttgcggaaag agcatccgaa aaccgataaa cgcgacgatt
     6541 aagccgcctg caattcgcag accgggaatc gaaatgccaa atgtatccat caccagttgc
     6601 ccggcgtaat acgccaccat catgatggca aatacgtaca ccgaggccat caacgactga
     6661 cgattacgtt cggcactgtt catgttgcct gccaggccaa gaaatagcgc gacagttgtt
     6721 aatgggttag ctaacggcag caacaccacc agccccaggc caattgcttt aaacaaatct
     6781 aacattggtg gttgttatcc tgtgtatctg ggttatcagc gaaaagtata aggggtaaac
     6841 aaggataaag tgtcactctt tagctaacct tgcatcgcat tgaccaaaac ttgaaccgat
     6901 ttagcaaaac gtggcatcgg tcaattcatt catttgactt atacttgcct gggcaatatt
     6961 atcctctgca actaattact tgccagggca actaatgtga aaagtaccag cgatctgttc
     7021 aatgaaatta ttccattggg tcgcttaatc catatggtta atcagaagaa agatcgtctg
     7081 cttaacgagt atctgtctcc gctggatatt accgcggcac agtttaaggt gctctgctct
     7141 atccgctgcg cggcgtgtat tactccggtt gaactgaaaa aagtgttgtc ggtcgacctg
     7201 ggagcactga cccgtatgct ggatcgcctg gtctgtaaag gctgggttga aaggttgccg
     7261 aacccgaatg ataagcgcgg cgtactggta aaacttacca ccagcggcgc ggcaatatgc
     7321 gaacaatgcc atcaattagt tggccaggac ctgcatcaag aattaacaaa aaacctgacg
     7381 gcggacgaag ttgcaacact tgagcatttg cttaagaaag tcctgccgta aacaaaaaag
     7441 aggtatgacg atgtccagac gcaatactga cgctattacc attcatagca ttttggactg
     7501 gatcgaggac aacctggaat cgccactgtc actggagaaa gtgtcagagc gttcgggtta
     7561 ctccaaatgg cacctgcaac ggatgtttaa aaaagaaacc ggtcattcat taggccaata
     7621 catccgcagc cgtaagatga cggaaatcgc gcaaaagctg aaggaaagta acgagccgat
     7681 actctatctg gcagaacgat atggcttcga gtcgcaacaa actctgaccc gaaccttcaa
     7741 aaattacttt gatgttccgc cgcataaata ccggatgacc aatatgcagg gcgaatcgcg
     7801 ctttttacat ccattaaatc attacaatag ctagttgata acgtgacaac gtcactgagg
     7861 caatcatgaa accactttca tccgcaatag cagctgcgct tattctcttt tccgcgcagg
     7921 gcgttgcgga acaaaccacg cagccagttg ttacttcttg tgccaatgtc gtggttgttc
     7981 ccccatcgca ggaacaacca ccgtttgatt taaatcacat gggtactggc agtgataagt
     8041 cggatgcgct cggcgtgccc tattataatc aacacgctat gtagttattg tcaggctctg
     8101 atcacagagc ctgacatctc cgtaatatct tcctcaagtc tcaaatattt tcgccattgt
     8161 tggcgatgag atcccgatac cagtaaaacg acgctttacg tatccgttcc agcgttccct
     8221 tcccttcgtt gtctttatcg acataaatca ttccgtagcg ctttttcatt tcccctgttc
     8281 cggcagaaac caggtcaatg cagccccacg gggtgtagcc aattaagtca acaccatctt
     8341 caactacggc ttttttcatt tcccgaatat gggaagcaaa gtaatcaatg cgatagtgat
     8401 cgttcaccgt gccgtcagct tgtctctggt caaccgcacc aaatccattt tcgacaataa
     8461 acagcggcaa ctggaaatga tcccagaacc agttgagtga ataacgtagc cctgccggat
     8521 caatttgcca tccccattcg gatgtatcga tataagggtt gctgaccaaa tctcgtggtt
     8581 caacataatc cagttgcggg ttatcgggag aaaattgtgt cgtaaaagac atgtagtagc
     8641 taaaaccgat aaagtcgaca catccactgg caagaatcgc gttatcttct ggtgtgatat
     8701 cgaggttgaa tcctttcctg gcaaagtaat tcagcatatg ttgcggataa tatccacgag
     8761 catgaacatc agtaaaccag taacgacgat gcatcgcttt cgtggccatc atcatatcgt
     8821 tgggtgcaca cgtcagagga tagatggggc acatggcgat catacagcca atgttaaatt
     8881 caggattgat cgattttcca gtctgtaccg ccagggcact ggcaactaac tcgtaatgta
     8941 ccgcctggta cattatttgt tcgcgctcat tgatatcttc ctctggcgaa tacaagatac
     9001 cggaattagt aaatggacac aggctttcgc tgaaattcac ctgattattg atttcgttaa
     9061 acgtcatcca gtactttact ttttctttat agcgcgtgaa gaccgttgat gcgaagcgga
     9121 taaaaaagtc gatcagctta cggtttcgcc agccaccata ttttgtcacc agatgataag
     9181 gcatctcaaa atgcgaaagc gtcaccacag gttccattcc ttgcttcagg cattcatcga
     9241 acagatcatc ataaaattgt aaaccctctt cattcggctc ctgttcgtca ccttgcggaa
     9301 agattcgtgt ccaggcaatg gaagttcgaa agcatttgaa tcccatctcg gcaaataact
     9361 gaatatctgt tttatagcga tgataaaaat caattgcttc atgattggga taattaagcc
     9421 cgtcgataac gccttctgtc acttcacgcg gcaccccgtg agcgccagca gtcatcacat
     9481 cagcgatact gatgcctttt cctccttcat tccagccacc ttccaactga tgcgcggcta
     9541 ccgcgccacc ccataaaaaa ccttttttaa atcctgacat acacaactcc ttaactaaaa
     9601 tagattacca ccagacttc
//
LOCUS       DAETQN010000151         9614 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.152, whole genome shotgun sequence.
ACCESSION   DAETQN010000151 DAETQN010000000
VERSION     DAETQN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.152"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..154
                     /locus_tag="K0W68_003767"
     CDS             <1..154
                     /locus_tag="K0W68_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI3075212.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
     gene            258..1172
                     /locus_tag="K0W68_003768"
     CDS             258..1172
                     /locus_tag="K0W68_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3075213.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAADGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1506..3785
                     /gene="ydeP"
                     /locus_tag="K0W68_003769"
     CDS             1506..3785
                     /gene="ydeP"
                     /locus_tag="K0W68_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBI3075214.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            4033..4230
                     /gene="safA"
                     /locus_tag="K0W68_003770"
     CDS             4033..4230
                     /gene="safA"
                     /locus_tag="K0W68_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HBI3075215.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4305..5066
                     /gene="ydeO"
                     /locus_tag="K0W68_003771"
     CDS             4305..5066
                     /gene="ydeO"
                     /locus_tag="K0W68_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBI3075216.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            5467..7149
                     /locus_tag="K0W68_003772"
     CDS             5467..7149
                     /locus_tag="K0W68_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBI3075217.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            7201..8358
                     /locus_tag="K0W68_003773"
     CDS             7201..8358
                     /locus_tag="K0W68_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBI3075218.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(8324..8446)
                     /locus_tag="K0W68_003774"
     CDS             complement(8324..8446)
                     /locus_tag="K0W68_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075219.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8661..>9614
                     /locus_tag="K0W68_003775"
     CDS             8661..>9614
                     /locus_tag="K0W68_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HBI3075220.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAF"
BASE COUNT         2815 a         2045 c         2047 g         2707 t
ORIGIN      
        1 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
       61 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      121 aagagctatc acggtgaatg gaaacgcaag ctagggaacg atcgaggcgc taatcaatgt
      181 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      241 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      301 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      361 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      421 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      481 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      541 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      601 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      661 ttggggcggc cgatggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt
      721 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
      781 ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
      841 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
      901 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
      961 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa
     1021 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1081 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt
     1141 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct
     1201 ccattcacga aaattgcgca gataattatt ttgtgaaggc tattatccta cacctgtaag
     1261 aaaatccgcg cacaattgaa gccagagtct tattttttat attgaaaaat atgttaattt
     1321 aaacactatg agagatgatg cttaacttat cgtatttaat ctattgttta acgataatag
     1381 attaaaaata gaaagagagt agtcccctac tcttatatat ccatgttggc gataatccct
     1441 ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc acacaggata
     1501 agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg ggtgctgtta
     1561 aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccatgatgtt attgccatgt
     1621 ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca gatcctaagc
     1681 acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg
     1741 ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta cttacctggg
     1801 gagatcacga gcttgaggct gcggggcgtc tcactcagcc tttgaaatat gatgccgtca
     1861 gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc gcacgccttc
     1921 aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact tccaatgaag
     1981 ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt cccgactgct
     2041 ccaacatgtg ccatgaaccg acaagcgtgg gtttggcagc gagtatcggt gtaggtaaag
     2101 ggaccgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt gggcataacc
     2161 ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa cggggagcga
     2221 aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa
     2281 acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac tacaacgtgc
     2341 gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg
     2401 atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt caaacgcata
     2461 ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac
     2521 gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat gccgctgccg
     2581 atcgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt acccagaatg
     2641 tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct ggtgcgggta
     2701 tctgcccact acgaggtcac tctaatgtac agggcgaccg aaccgtcggt atcaccgaga
     2761 aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg
     2821 cacctggaca tgctgcaatt gccagcatgc aagcaatatg cacggggcag gctcgagcat
     2881 tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg agcgctgtac
     2941 cgttaacgca attagatttg gcggtacacg tagccactaa gcttaaccgc tctcatctgc
     3001 tgaccgcacg gcatagctat attctgccgg ttctggggcg tagcgagatt gacatgcaaa
     3061 aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat gcctcgcgtg
     3121 gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg
     3181 cgcaggcagc actaccccac agcgtggtag cctgggagta tctggtggaa gattatgatc
     3241 gcattcgcaa tgacattgaa gctgtgttac cagagttcgc cgactataac cagcgcatcc
     3301 gacatcctgg aggttttcac ctgataaatg cagctgctga aaggcgctgg atgacgccgt
     3361 caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct tcagcgttta
     3421 acagtaagct ggtcatggcg acagtacgta gccacgatca gtacaacacg acgatttatg
     3481 gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta
     3541 aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc
     3601 cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt taccctatgg
     3661 ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaact cttgataacc
     3721 acgatccatt aagtggcatt cctggttata aaagtattcc ggttgaatta gaaccatcaa
     3781 attaattcgc ctcctcattt cttctgctgt catccgcaca gcagaagaat ttctcattga
     3841 ctattatcgc gcaatttgct cacattgatt aaattaaacc acacactata agatataaac
     3901 ctctgcctac agctgtaaga aactccgctc agtactgaag tgccagtcct atttcctctt
     3961 ttctccagcc tgttatatta agcatactga ttaacgattt ttaacgttat ccgctaaata
     4021 aacatatttg aaatgtatgc gaccacagtg aaaaacaaaa tcacgcaaag agacaactat
     4081 aaagaaatca tgtctgtaat tgtggttgtc ttattactga cccttacgtt gatagccatt
     4141 ttttcggcaa ttgatcagct gggtatttca gaaatgggtc gcattgcaag agatcttaca
     4201 catttcatta tcaatagttt gcaagactga aaataaacag caaattataa atatgaaatg
     4261 ttaaaaaagt atcgataaaa actttattgt tttaaggaga taaaatgtcg ctcgtttgtt
     4321 ctgttatatt tattcatcat gccttcaacg ctaacatttt agataaagat tacgccttct
     4381 ctgacggcga gatcctgatg gtagataacg ctgttcgtac gcattttgaa ccttatgagc
     4441 ggcattttaa agagatcgga tttactgaaa ataccattaa aaaatatcta caatgcacta
     4501 acatccagac agtgacggtg cctgttcctg cgaagttttt acgtgcttca aatgtaccga
     4561 ctggattgct taatgaaatg attgcttatc ttaactcgga agaacgcaat catcataatt
     4621 tttcagaact tttgcttttt tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta
     4681 cactattaac taacggtgtg ctatccgttt ccggaaaagt gagaaatatt gtcaacatga
     4741 agctggcgca cccatggaag ctgaaagata tttgtgactg cctgtacatc agtgaaagcc
     4801 tgttgaagaa aaaactaaag caagagcaaa cgacattctc acagattcta ttagatgcca
     4861 gaatgcagca cgcaaaaaat ttgatacgcg tagaaggttc agtcaataaa attgccgaac
     4921 aatgtggtta tgccagtaca tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca
     4981 gtccgaagag agtttctaag gagtaccgtt gtcaaagtca catgggtatg aatacgggca
     5041 acacgatgag tgctttagct atttgattat ttgctaacga gtagtcaacc acgcacgctg
     5101 cgtaagaatt aaatggggca gccattccct gccccgcgtt gtttttaggc gatatattta
     5161 ttgaaataaa taagtgacat ccatcacata tttatgcact tgcataacct gttgcagatt
     5221 atttatgatc tcaattcggc attttgtcag taaaatgcaa taatttatta aatatcaata
     5281 aattagttgt ttatcggcga gaaattactt aatagaacag aaagtaatgt caacgcttta
     5341 tggactgtta tttccctttt tttagctaaa tctgctatct ctttatgtga ctaacttcac
     5401 ttacatccac ttatttctct tcgtaaaatt acttcggaat taagtacaat aagaagagga
     5461 acatttatga agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg
     5521 gcatctggta tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc
     5581 aaaacaaacg ttgctttctc agattttacg ccgactgaat acagtaccaa aggaaagcca
     5641 aatattatcg tactaaccat ggatgatctt ggttatggac aacttccttt tgataaggga
     5701 tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata
     5761 gataaagcca ttgaagctgc acaaaaatca acgccgacgc tcctttcatt aatggatgaa
     5821 ggcgtacgtt ttactaacgg ctatgtggca cacggtgttt ccggcccctc ccgtgccgca
     5881 ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga tgctcaggat
     5941 ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg ttattacact
     6001 gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa
     6061 caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg gcaacctcaa
     6121 aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata ttacaactcc
     6181 ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta
     6241 accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt
     6301 tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga tcaatatcag
     6361 aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt ttattctgtt
     6421 gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta tgacaataca
     6481 attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc gctgaacggg
     6541 gcgcaaaaag gctataagag tcagacctat cctggcggta ctcacacccc aatgtttatg
     6601 tggtggaaag gaaaacttca acccggtaat tatgacaagc tgatttccgc aatggatttc
     6661 tacccaacag ctcttgatgc agccgatatc agcattccaa aagaccttaa gctggatggt
     6721 gtttccttgc tgccctggtt gcaagataag aaacaaggcg cgccacataa aaatctgacc
     6781 tggataacct cttattctca ctggtttgat gaggaaaata ttccattctg ggataattac
     6841 cacaaatttg ttcgccatca gtcagacgat tacccgcata accccaacac tgaggactta
     6901 agccaattct cttatacggt gagaaataac gattattcgc ttgtctatac agtagaaaac
     6961 aatcagttag gtctctacaa actgacggat ctacagcaaa aagataacct tgccgccgcc
     7021 aatccgcagg tcgttaaaga gatgcaaggc gtggtaagag agtttatcga cagcagccag
     7081 ccaccgctta gcgaggtaaa tcaggagaag tttaacaata tcaagaaagc actaagcgaa
     7141 gcgaaataac taaaccttca tgcggcggat ttttccgccg ccttattgag cgagatagcg
     7201 atgcacgtta cagccaagcc ctccagtttt caatgtaatc tcaaatgtga ttactgtttt
     7261 taccttgaaa aagagtcgca gtttactcat gaaaaatgga tggatgacag cactctgaaa
     7321 gagttcatca aacaatatat cgaagcgtct ggcaatcagg tctattttac ctggcaaggc
     7381 ggtgaaccca ctctggctgg cctggatttt ttccgtaaag ttattcacta tcaacaacgc
     7441 tatgcaggcc aaaaacgtat ttttaatgca ttacaaacga atggcatttt attgaataat
     7501 gaatggtgtg cctttctcaa agaacatgaa tttctggttg gtatctcgat cgatggcccc
     7561 ctggagttac atgaccgtta cagacgcagt aattcaggta acggtacttt tgcaaaagtg
     7621 atagcagcca tcgagcgtct gaaatcatat caagtagagt ttaatacgtt aaccgtcatt
     7681 aataacgtta atgtccatta ccctcttgag gtttatcatt ttttaaaatc tatcggcagt
     7741 aaacatatgc aatttatcga attgctagaa accgggacgc cgaatattga tttcagtggt
     7801 catagtgaga acacattccg tatcattgat ttttctgtgc ctcccacggc ttatggcaag
     7861 tttatgtcaa ccatttttat gcaatgggtt aaaaacgatg tgggtgaaat tttcattcgt
     7921 cagtttgaaa gctttgtcag ccgttttttg gggaatgggc atacaagttg tattttccag
     7981 gagtcctgca aggataatct ggttgttgaa agtaatggag acatttacga atgcgaccat
     8041 tttgtctatc cacagtacaa aattggaaac attaataaat ctgaactcaa aacgatgaac
     8101 agtgtacaac tgacagcgca aaaaaaacgg atttcagcga aatgtcagca atgtgcatat
     8161 aaacctatct gcaatggcgg ttgtcctaag catcgtatta ctaaagtaaa caatgagact
     8221 gtttcttatt tttgcgaagg ttataaaatc cttttttcaa ccatggtacc ttatatgaac
     8281 gccatggttg agttagctaa gaacagagta ccgctttacc acattatgga tgttgcaaaa
     8341 caaatggaga ataattaata acgctctctt ttaactccat tcttagcctc cttcttatga
     8401 ccttaacaag gcacccgaca taaattcctg tcgggtgttt cgtcatcatc atcttccctt
     8461 gcgaaaatcc tggcttacgt ggtatgcgtc agggcattag caataccctt gattatcaat
     8521 agcataacca taaccataac cataaccact gaaaattaaa aatttctata tatttcatat
     8581 tgttaaatga tacttaaata aatttcatca gtaaatcatt gccatagata atcattggat
     8641 ggcacaatca attttcgcta atgataacca ttcccattac gttttatatg ttgatagcaa
     8701 agtatctctg tctgctgaag cccttttggc tgcgtaagaa taataaaact tcagtgttgt
     8761 taatcattat tatcctcgca atgatccttg gcgtggttaa aattcaggtc tggttaaacg
     8821 actggaataa tgattttttc aatgcgctaa gccagaaaga aaccgataag ctttggcagc
     8881 ttgttctttg gttccctgcc ctgctgggga tctttgtatt aatatccgta aacaaaacat
     8941 ggttaattaa actactgact attcgctggc gcgaatggtt aacagattac taccttaatc
     9001 gctggtttgc agataaaaac tactacttca cgcagatcta cggcgagcat aaaaatacag
     9061 ataaccctga ccaacgtatc gctgaagaca ttctcttact gatcagcaaa acgctcagtc
     9121 tttcatttgg cttcatccag tcacttagca tgctgatcac ctttaccgtt attctttggc
     9181 aaagcgcggg tacgctctca ttcactgttg gtggaacaga atggaatatc caggggtata
     9241 tggtctatac cgtcgtgctc attgtgatcg gtggaacttt atttacccat aaggtgggta
     9301 aacgtattcg tccgcttaat gtggaaaaac aacgtagcga agcgacattt cgaactaatc
     9361 ttgtgcagca taataagcag acagaactga ttgcgctaag caatgctgag tctctacaac
     9421 gccaggagtt gcgcgacaat tttcatacca ttaaagagaa ctggcatcgt ttaatgaatc
     9481 gtcagcggtg gcttgattac tggcaaaata tttattcgcg ctcgcttagc gttcttccct
     9541 actttctgtt attgccgcag tttattagcg ggcagatcaa tctgggcgga ctgatgaaat
     9601 cgcgccaggc attt
//
LOCUS       DAETQN010000152         9566 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.153, whole genome shotgun sequence.
ACCESSION   DAETQN010000152 DAETQN010000000
VERSION     DAETQN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.153"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            20..1345
                     /locus_tag="K0W68_003776"
                     /pseudo
     CDS             20..1345
                     /locus_tag="K0W68_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            1348..2676
                     /gene="ssnA"
                     /locus_tag="K0W68_003777"
     CDS             1348..2676
                     /gene="ssnA"
                     /locus_tag="K0W68_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBI3075221.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2727..3506
                     /locus_tag="K0W68_003778"
     CDS             2727..3506
                     /locus_tag="K0W68_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HBI3075222.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3503..6442
                     /locus_tag="K0W68_003779"
     CDS             3503..6442
                     /locus_tag="K0W68_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HBI3075223.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6375..6689
                     /locus_tag="K0W68_003780"
     CDS             6375..6689
                     /locus_tag="K0W68_003780"
                     /inference="COORDINATES: protein motif:HMM:NF019581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBI3075224.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6725..7075
                     /gene="ghxQ"
                     /locus_tag="K0W68_003781"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6725..7075
                     /gene="ghxQ"
                     /locus_tag="K0W68_003781"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7144..7632)
                     /locus_tag="K0W68_003782"
     CDS             complement(7144..7632)
                     /locus_tag="K0W68_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBI3075225.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7632..9551)
                     /gene="ygfT"
                     /locus_tag="K0W68_003783"
     CDS             complement(7632..9551)
                     /gene="ygfT"
                     /locus_tag="K0W68_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HBI3075226.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2307 a         2564 c         2492 g         2203 t
ORIGIN      
        1 tgatccttaa ctgatcggca ttattcctca gttccgtatt cctgcagagt taattcagca
       61 cgatatcgat tttgttgccg ctcacggcgt gaaatttgag tatggctgct cacccgattt
      121 aaccgttgaa cagttaaaaa atcagggctt ccactatgtt ctgattgcca ccggcactga
      181 taaaaatagc ggtgtgaaac tggcgggcga caaccaaaat gtctggaaat cactcccctt
      241 cctgcgtgaa tacaacaagg gtacagcgct caagctgggc aaacatgtgg tcgttgtcgg
      301 ggcgggtaac accgcaatgg actgcgctcg tgcggcgtta cgcgttccag gcgtagaaaa
      361 agcaacgatc gtttaccgtc gttcactaca agagatgccc gcatggcgcg aagagtatga
      421 agaagcgttg cacgacggcg tagagttccg tttcctgaat aatccggaac gtttcgatgc
      481 tgatggcacc ttaaccttgc gcgttatgtc gcttggcgaa ccggatgaga aaggtcgtcg
      541 ccgtccggtt gaaaccaacg aaacagtaac actgcatgta gacagcctga tcaccgccat
      601 tggtgaacag caggatactg aagccctgaa tgcgatgggc gtgccgctgg acaaaaacgg
      661 ctggccagac gtcgaccata atggcgaaac tcgtctgact gacgtcttta tgatcggcga
      721 cgtacagcgc ggaccatcct ccattgtcgc tgctgtcgga accgcgcgtc gggcgaccga
      781 tgccatcctt agtcgggaaa atatccgttc ccaccagaac gataaatact ggaacaacgt
      841 caatccagcg gaaatctatc aacgtaaagg cgatatctct atcacgctgg tgaacagtga
      901 cgatcgtgac gcgtttgtcg cccaggaagc cgctcgctgc ctcgaatgta actacgtttg
      961 caacaagtgt gtggatgtct gcccgaaccg cgccaacgtc tccattgcag tccctggctt
     1021 ccagaataga ttccagacgc tgcacctcga cgcttactgt aacgaatgcg gcaactgcgc
     1081 tcagttctgt ccgtggaacg gtaaaccata cgaagacaaa atcaccgtct tcagcctggc
     1141 gcaagacttt gataacagca gcaccccagg cttccttgtg gaagattgcc gggtacgagt
     1201 acgtctgaat aaccaaagct gggtgttaaa catcgacagc gaaggtcagt ttaacaacgt
     1261 accaccggag ctgaacgata tgtgccgcat catcagccat gtccaccagc atcatcatta
     1321 tctgctgggc cgcgtggagg tgtaatcatg ttgattctga agaatgtcac cgcagtgcag
     1381 ttacacccgg cgaaagtgca ggaaggcgtt gatatcgcta tcgaaaatga cgtgattgtc
     1441 gctatcggcg atgccctgac gcaacgctac cccgatgcca gctacaaaga gatgcatggt
     1501 cggattgtga tgccgggaat tgtctgctcg cacaaccatt tttactcgga gctttcccgc
     1561 ggaattatgg caaacatcgc cccttgcccg gatttcatct caacgctgaa aaatctctgg
     1621 tggcggctcg atcgcgccct tgatgaagag tcgctctatt acagcggact gatttgttcc
     1681 ctggaagcga ttaagagcgg atgtacatcg gttatcgatc accatgcctc gccggcgtat
     1741 atcggcgggt cgctctccac attgcgcgac gcatttttaa aagttggcct gcgcgcgatg
     1801 acctgttttg aaactactga ccgtaacaac ggcatcaaag agttgcagga aggtgtagaa
     1861 gaaaacatcc gtttcgcccg tttgattgat gaggcgaaga aagcgacaag cgagccgtat
     1921 ctggtggaag cacatatcgg tgctcacgcg ccgtttaccg tgccggatgc cggtctggag
     1981 atgctgcgtg aagccgtgaa agccacaggc cgtggtttgc atattcacgc tgcggaagac
     2041 ctttacgacg tttcctacag tcaccactgg tacggcaaag acctgctggc acgactggcg
     2101 caattcgacc tcatcgacag caaaacgctg gtcgctcatg ggctgtactt gtcgaaagat
     2161 gacatcgccc tactcaatca gcgcgatgcg ttcctggtgc ataacgcccg ttcaaacatg
     2221 aacaaccatg tcggctacaa ccatcacctt agcgacatcc gcaacctggc attgggaacg
     2281 gacggcattg gttcggacat gtttgaagag atgaaatttg ccttctttaa acatcgcgat
     2341 gcgggtggtc cgctgtggcc tgacagtttt gccaaagccc tgactaacgg taacgaactg
     2401 atgagccgca actttggcgc gaaatttggg cttctggaag ccggttacaa agctgatttg
     2461 accatttgcg attacaactc gccgacgccg ctgctggcag acaatatcgc cgggcatatc
     2521 gctttcggta tgggctcagg cagcgttcac agcgtgatgg tcaatggtgt gatggtctat
     2581 gaagaccgtc agtttaactt cgattgcgat tccatttatg cacaagccag aaaagccgct
     2641 gccagtatgt ggcgtcggat ggatgcgctg gcataaatga cagatgccct cttcccgcag
     2701 ggaagagggt taaataagga aagaagatga ttgaacaatt tttcaggccc gactctgtcg
     2761 aacaggcgct ggaactgaag cgccgctacc aggatgaagc cgtctggttc gccgggggca
     2821 gcaaactcaa tgctacacca acccgtaccg ataaaaagat tgccatttcc ttacaggatc
     2881 tggaactgga ctgggttgac tgggataacg gtgcactgcg gattggtgca atgtctcgct
     2941 tgcagccact gcgtgatgcg cgctttattc ctgcagcact gcgtgaagcc ctcggttttg
     3001 tttactcacg ccatgttcgt aatcagtcga ccattggtgg tgaaatcgcc gcccggcagg
     3061 aagagtcagt gctgcttccc gtcctgctag cactggatgc tgaactggtt tttggcaacg
     3121 gcgaaacgct gtcaatcgag gactacctgg cctgcccatg cgatcgcctg ctaaccgaaa
     3181 ttatcattaa agatccgtat cgcacctgtg cgacccgcaa aattagccgt tctcaggcag
     3241 gtttaaccgt cgtgacggca gccgttgcaa tgacagacca cgacggtatg cgaattgcgc
     3301 tggatggcgt cgccagtaaa gcactgcgtc tgcacgatgt cgaaaaacaa aatctggaag
     3361 gcaatgcact tgaacaggct gtcgccaacg ccattttccc gcaggaagat ttgcggggca
     3421 gcgtggccta taaacgctat atcacgggag ttctggtagc cgacctgtat gccgactgcc
     3481 aacaggctgg ggaggaagcc gtatgatcat ccactttact ttaaatggcg cgcctcagga
     3541 gctaaccgtt aatccaggcg aaaacgtgca aaagctgttg tttaacatgg gaatgcactc
     3601 tgtacgcaac agtgatgatg gcttcgggtt tgccggttct gacgcaataa tctttaacgg
     3661 taatatcgtt aacgcgtcct tgcttattgc cgcacagtta gagaaggcag atattcgtac
     3721 cgcagaatct ctgggtaaat ggaacgagtt aagtctggtt caacaggcaa tggttgatgt
     3781 tggcgtggtg cagtctggtt ataacgatcc agctgcagct ctgattatca ccgatcttct
     3841 cgatcgcatc gccgcaccta cccgcgaaga gatcgacgac gcgctttctg gtttgttcag
     3901 ccgcgatgca ggctggcaac aatactatca ggtcattgaa ctggcggttg cacgtaaaaa
     3961 taatccgcag gccaccattg atatcgctcc gactttccgt gacgaccttg acgtcattgg
     4021 caagcattat cctaaaactg atgccgcgaa aatggtgcag gcgaaaccct gctatgttga
     4081 agactgcgta acggctgacg cctgcgtcat taaaatgtta cgtagcccac acgctcacgc
     4141 actgattact catctggatg tcagcaaagc tgaagcctta ccgggcgtcg ttcacgttat
     4201 tactcacctg aattgccctg atatctacta taccccgggg ggtcagagcg caccggaacc
     4261 gtcaccgctt gaccgccgta tgttcggcaa gaaaatgcgt cacgtcggcg atcgtgttgc
     4321 tgcggtcgtc gcagaaagtg aagaaattgc gctcgaagca ctgaagctca tcgacgttga
     4381 atatgaagtg cttaagccgg taatgtcgat cgacgaagca atggcggaag atgcgcctgt
     4441 cgtgcacgat gaaccggtgg tgtatgttgc tggtgcgcca gatactctgg aagacgataa
     4501 cagccatgca gcccagcgcg gcgagcatat gatcatcaac ttcccgatcg gttctcgccc
     4561 tcgcaaaaat atcgccgcca gtattcatgg tcatattggc gatatggaca aaggctttgc
     4621 cgatgccgat gtgatcattg agcgaaccta taactcaacg caggcacagc agtgcccgac
     4681 tgaaacacat atctgcttta cccggatgga cggcgatcgt ctggttatcc acgcctccac
     4741 ccaggtacca tggcacttac gccgccaggt cgcgcgcctc gtgggcatga aacagcataa
     4801 agttcatgtc attaaagagc gagttggcgg cggttttggt tccaaacagg acatcctgct
     4861 ggaagaagtg tgcgcctggg caacctgcgt gaccgggcgt ccggtactat tccgctacac
     4921 ccgtgaagaa gagtttattg ctaacacctc tcgtcacgtc gcgaaagtca ccgtcaaact
     4981 gggagcgaaa aaagatggtc gcctgacggc agtgaagatg gatttccgcg ccaacactgg
     5041 cccttacggc aaccactcac tcaccgtacc gtgtaacgga ccggcgctgt cgctgccgtt
     5101 atatccgtgc gataacgtcg atttccaggt caccacctac tacagcaaca tttgcccaaa
     5161 tggtgcttat cagggttatg gcgcaccgaa aggtaacttc gctatcacca tggcattagc
     5221 ggaactggct gaacagttac agatcgacca actggaaatt atcgaacgta accgggtaca
     5281 cgaagggcaa gagctgaaaa ttctcggtgc aatcggtgaa ggtaaagcgc cgacctccgt
     5341 tccttccgcc gccagctgtg cactggaaga gatcctgcgt cagggtcgcg agatgatcca
     5401 atggtcgtca ccgaaaccgc aaaatggtga ctggcacatc ggtcgcggcg tcgccattat
     5461 catgcagaaa tcgggtatcc cggatatcga tcaggctaac tgcatgatca aactggaatc
     5521 ggacggcacc tttatcgttc attctggcgg tgcggatatt ggtactggtc tggatactgt
     5581 agtgacgaaa ctggcagcag aagtgctgca ctgcccaccg caggacgtgc atgttatctc
     5641 cggtgatacc gatcatgcgt tgtttgataa aggcgcatat gcctcgtccg gtacttgctt
     5701 ctcgggtaac gcggcgcgtt tggcagcgga aaatctacgg gagaaaattc tgttccacgg
     5761 cgcgcaaatg ttgggtgagg cagtggcaga tgttcaactg gcaacgccgg gcgtcgtgcg
     5821 cggcaagaaa ggcgaagtta gtttcgggga tattgcccat aaaggcgaaa ccggcatcgg
     5881 ctttggttca ctggtgggaa ctggcagtta tatcacgcct gatttcgcct tcccgtatgg
     5941 cgcaaacttc gctgaagttg ccgtcaacac gcgtacgggt gaaatccgcc tggataaatt
     6001 ctacgccttg ctggactgcg gtacaccggt caacccggaa ttagcattgg gacaaatcta
     6061 cggtgctacc ctgcgagcta tcggccacag tatgagcgaa gagatcattt atgacgccga
     6121 aggtcacccg ttaacgcgtg atttacgcag ttacggcgca ccgaaaattg gtgacattcc
     6181 gcgtgatttc cgcgctgtgc tggtgccgag cgacgataaa gtcggcccgt tcggggcgaa
     6241 atcgatctcg gaaatcggtg taaatggcgc agctccggcg attgctaccg caattcacga
     6301 tacatgcggc atctgggcag aagaaagtca aagtgggcat gggaacggat atcggtgccg
     6361 gaacaacttt caacatgctg caaacgctga acgaagccta caaagtgttg caattacaag
     6421 gctatcgcct ctcggcatat gaagcgtttt acctggccac gctcggcgga gcgaaatctc
     6481 tgggccttga cgatttgatt ggcaactttt tacctggcaa agaggctgat ttcgtggtga
     6541 tggaacccac cgccactccg ctacagcagc tgcgctatga caactctgtt tctttagtcg
     6601 acaaattgtt cgtgatgatg acgttgggcg atgaccgttc gatctaccgc acctacgttg
     6661 atggtcgtct ggtgtacgaa cgcaactaat aataaaactt taacatcctc gtgaggacat
     6721 cattatgtct ggagacatcc tacaaacacc ggacgcacca aagccacagg gcgcgctgga
     6781 taattatttt aaaattaccg ctcgtggcag taccgttcgt caggaagtac tggctggctt
     6841 aacgaccttt ctggccatgg tttattccgt tatcgtcgtt ccgggaatgc tgggcaaagc
     6901 agtttttcct cccgcagctg tgtttgttgc cacctgtctg gtcgcgggct tcggctcgtt
     6961 gctgatgggg ttgtaggcca atttgccaat ggcgattggt tgtgcgattt ccttgacggc
     7021 atttaccgca ttcagtctgg tactcgggca acaaattagc gttcctgtcg cactggtcgc
     7081 attttacgcg ggtggttggg caatctaata gattctccgc gcctttcagc gcggagattt
     7141 ctttcacaga ggattctcac cgctggcaac aatacgctgc tggcggactc gcctgagttc
     7201 tctttcagta agcaactgca gcgcctgtgt cgggcaagat tcaacacagg ctggcccctc
     7261 ttcccgctgt tcacagagat cgcatttcac aatttgttgt cgggtatccc ccggcaacga
     7321 ctgaatggtg atcattccaa acgggcacgc actgacgcaa ctctgacagc caatacaacg
     7381 ggcagaattg gtctgcacca cctgctcgcc catcgtcagc gcccccacgg ggcaagcgcc
     7441 aacacaaggg gcgttttcac actgatggca catcaccggc gcactgatgc tgtccagtcg
     7501 ctgtaccttc agccggggta aaaagacatc ggcattcaac tcctgttctg aagggtgagc
     7561 gaccacacag gctacttcac aagtacggca gccaatacag tcagccggat taacgataat
     7621 taacgatttc atcacgatgc cttcgtatca aacagagtta acatatcgcg cgccgcctgc
     7681 cttcctgcgg ccattgcagt gacaaccaga tccgcgccat gaactgcatc accaccagca
     7741 aagacttttt tcagatgcgt ctgggtaggt aaatacccga cgtcaccggt ttgaatcagg
     7801 ccccatttat cgagtttaat tccgctgccc tgcaaccacg gcatggcatg cgcctggaaa
     7861 ccaaaggcca taatcagaac atcggcgggc aattcaaact ctgaacccgc taccggacga
     7921 ggacgacggc gaccatccgg ccccggctca cccatggcgg tacgaatcag gcccaccgca
     7981 gttaaacgcc catcttcgtc acaagcgata tattgcggtt gaacattgaa ctgaaactcg
     8041 acaccttcct cgcgcgcatt aaccacctct ttgcgcgagc ccggcatact gacttcatca
     8101 cgacgatacg cgcaggtcac gctggcagca ttgaggcgaa tggaagtccg caaacaatcc
     8161 attgtcgtat cgccaccgcc caataccacg actcgcttac cttccacgtc cgtcagcgga
     8221 tactcttcag actccggcaa tcccatgagc tggcgggtat gggcagtcag gaacggtaga
     8281 gcctgaatga caccgggcgc atcttcatgc ggcagatctg ctcgcatcat cccgtaagtc
     8341 cccacgccga tgaaaactgc atcatattca gaagttaaat cgctaaaggt aatatcgcgg
     8401 ccaatttcac agttaagatg aaagtcgatt cccattgcgg tgaatatctc tcgccgctgg
     8461 cttaataccg ttttatcgag tttgaaagga ggaatgccaa aagtcagcat accgccaatt
     8521 tctggatggc gatcaaagac atcaacctga actcccgcgc gcgccagaat atcagcacac
     8581 cctaaccctg ctggcccagc gccaatcacc gccacttttt cgatacgggg aacaactttg
     8641 ctgacatcgg gacgccagcc catcgccagc gcggtatcgg tgatgtagcg ttccagatta
     8701 ccgatagaga ctgcgccaga gtgatctttc aaagtacatg cgccttcaca aagacggtcc
     8761 tgtgggcata ccctgccgca gatttctggt aaggaactgg tctggtggca aagttctgcc
     8821 gcttcaataa tctttccttc ctgtaccaga cggatgtaat ccggaatagc gttatgcagc
     8881 gggcaatgcc agttgcagtt agctttttcg gcacaataaa cacagcggtc actctcataa
     8941 gtcgcttgca gtggatccag cccgcaatag atttcgccaa agtgggtttt ccgttcactc
     9001 gctgaaattt tatctgcgcc tttacgcgag ttaacgggga gcaacgctgc actgcgagat
     9061 ggctgagcgt ctgatgacgc ttttcctgct gccgttttgc gctggcgagc cacctttatc
     9121 cactgtaacc ctttatcgtc catcagtcgt aacgcctgtg ttgggcagac ttcaatacag
     9181 gcttgcgtgc cggaactgcg ctggttacaa aggtcgcatt tctgcgcaat cgtatcgacc
     9241 atctcaacga cgccaaaggg gcaagcgatt gcgcatcttt tacagccaat acatttttgc
     9301 tcgtccagtt gtacgctatc ggactggaaa gtcagagcat taaccggaca agccgtaaca
     9361 caaggggcat tgttgcagtg atggcaggcc accggattcg cagcctggcc tttccctaca
     9421 acgtggatac gcggtcgaaa gtcactgtga ctcagcggcc agttttcttg attgtgtgcc
     9481 accgcacagg caatttcaca agcatgacag cctatacatt ccgcagcttc agcagcgata
     9541 aacttattca tttgcatccc tttcat
//
LOCUS       DAETQN010000153         9500 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.155, whole genome shotgun sequence.
ACCESSION   DAETQN010000153 DAETQN010000000
VERSION     DAETQN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.155"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(149..1231)
                     /locus_tag="K0W68_003784"
     CDS             complement(149..1231)
                     /locus_tag="K0W68_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HBI3075227.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3024)
                     /locus_tag="K0W68_003785"
     CDS             complement(1228..3024)
                     /locus_tag="K0W68_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBI3075228.1"
                     /translation="MNVQHFDFFTDNEFSNLERYQTCKAMAKGYAGNYKTGQSRLMHA
                     RSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAERTGELIAQFPAE
                     DAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDFSHASVIDPACGG
                     GAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLVLLESVLMPLCVK
                     VKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKYSRSLFGHANLYG
                     LFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYALDFVTDRDGVFD
                     DVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKGGATWLLPRVKDD
                     ACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPLVWAESITSAGFR
                     FSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAILPQGFIDETGGV
                     VVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVSAYELNSIPLPSL
                     EQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="K0W68_003786"
     CDS             complement(3275..3631)
                     /locus_tag="K0W68_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075229.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="K0W68_003787"
     CDS             complement(3689..4111)
                     /locus_tag="K0W68_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBI3075230.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="K0W68_003788"
     CDS             complement(4127..4852)
                     /locus_tag="K0W68_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HBI3075231.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="K0W68_003789"
     CDS             complement(4874..5620)
                     /locus_tag="K0W68_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI3075232.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="K0W68_003790"
     CDS             complement(5627..6691)
                     /locus_tag="K0W68_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HBI3075233.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGGPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="K0W68_003791"
     CDS             complement(6763..7188)
                     /locus_tag="K0W68_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBI3075234.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="K0W68_003792"
     CDS             complement(7185..7448)
                     /locus_tag="K0W68_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3075235.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="K0W68_003793"
     CDS             7560..8060
                     /locus_tag="K0W68_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3075236.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="K0W68_003794"
     CDS             8080..8274
                     /locus_tag="K0W68_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBI3075237.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="K0W68_003795"
     CDS             8274..8564
                     /locus_tag="K0W68_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBI3075238.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8844..8996
                     /locus_tag="K0W68_003796"
     CDS             8844..8996
                     /locus_tag="K0W68_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBI3075239.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            8996..9373
                     /locus_tag="K0W68_003797"
     CDS             8996..9373
                     /locus_tag="K0W68_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075240.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
BASE COUNT         2443 a         2493 c         2016 g         2548 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatc tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ccggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggcc ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatac tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaaat aatgatgcta ttgtttcagg cgtcaaccca ccccgcccca cagaatgcag
     8701 ggcaatactt cgagttacca gacagtggtc aggggttaag tagccagccc gaggcgtaag
     8761 aacatgacgg cagggttcaa tctttaacta tgcagcaggt tttttgttcc gccaccccgg
     8821 cgttaagggg aaatgaggtc agcatggata ctatcgatct tggcaacaac gaatctctgg
     8881 tgtgcggcgt gttccctaac caggatggaa cgtttaccgc catgacgtat accaaaagca
     8941 aaacgtttaa aaccgaatct ggcgcgcgtt gctggttagc cagaaacact gactgatgag
     9001 gctgacgatg gaatttaaag atttaccgtc tgatgtacag aaaacagcag ctcatacatt
     9061 gcattctgtg ctgcgagaaa tcgggaaaga tattgcaagc gagccagcaa aagatctggc
     9121 ccggaaaatc aagaccgctt tcgttgagct ttataatgtt ggcactgact ctgaaactgt
     9181 cgagaccaag accgtaagtt caccaatatt ctcacttgag ccaagcgtat tatcaggtga
     9241 aatatgcacc gagatttcca gcgaactgct tccggtaata cgagaagcaa ttcgccgtcg
     9301 cggtttggat ggaagttatg atcatgacgt tctgcaagtt ctcaggacaa tggtgacttc
     9361 actggggatt tgatccctgc gtcctctaag cttttgatat agcggtcctc caagaagcgg
     9421 taaattttgt cgaaatcatc ttttccctct ggtttattca gaggattaca ggacccgtaa
     9481 gtagcggcat acatttcaac
//
LOCUS       DAETQN010000154         9432 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.156, whole genome shotgun sequence.
ACCESSION   DAETQN010000154 DAETQN010000000
VERSION     DAETQN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.156"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            205..1071
                     /gene="folD"
                     /locus_tag="K0W68_003798"
     CDS             205..1071
                     /gene="folD"
                     /locus_tag="K0W68_003798"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBI3075241.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1073..1285
                     /gene="ybcJ"
                     /locus_tag="K0W68_003799"
     CDS             1073..1285
                     /gene="ybcJ"
                     /locus_tag="K0W68_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBI3075242.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1393..1914
                     /locus_tag="K0W68_003800"
                     /pseudo
     CDS             1393..1914
                     /locus_tag="K0W68_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="K0W68_003801"
     CDS             complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="K0W68_003801"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBI3075243.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3509..4003
                     /gene="ppiB"
                     /locus_tag="K0W68_003802"
     CDS             3509..4003
                     /gene="ppiB"
                     /locus_tag="K0W68_003802"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBI3075244.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4006..4728
                     /gene="lpxH"
                     /locus_tag="K0W68_003803"
     CDS             4006..4728
                     /gene="lpxH"
                     /locus_tag="K0W68_003803"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBI3075245.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4846..5355
                     /gene="purE"
                     /locus_tag="K0W68_003804"
     CDS             4846..5355
                     /gene="purE"
                     /locus_tag="K0W68_003804"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBI3075246.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5352..6419
                     /gene="purK"
                     /locus_tag="K0W68_003805"
     CDS             5352..6419
                     /gene="purK"
                     /locus_tag="K0W68_003805"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBI3075247.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6556..7449)
                     /locus_tag="K0W68_003806"
     CDS             complement(6556..7449)
                     /locus_tag="K0W68_003806"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBI3075248.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7446..8099)
                     /locus_tag="K0W68_003807"
     CDS             complement(7446..8099)
                     /locus_tag="K0W68_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBI3075249.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8110..9369)
                     /locus_tag="K0W68_003808"
     CDS             complement(8110..9369)
                     /locus_tag="K0W68_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBI3075250.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2102 a         2673 c         2498 g         2159 t
ORIGIN      
        1 tagacaagga aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg
      121 aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac
      181 gtaacagatg gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg
      241 cagcaggtgc gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg
      301 gcaccaggac tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca
      361 agcaaacgca aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa
      421 accaccagcg aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc
      481 gatggcattc tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa
      541 cgtattcacc cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc
      601 cagcgcgcgc cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt
      661 tacaacattg ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt
      721 cgtccaatga gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc
      781 actaaaaatc tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag
      841 ccgggcttta ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc
      901 aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc
      961 gcctcataca ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt
     1021 gaaaacacgc tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac
     1081 attttcttta ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg
     1141 gagcgaaagc ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg
     1201 cgcggttgaa acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg
     1261 tcacagcgta caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc
     1321 ttttcagact tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc
     1381 gaagcgacca tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta
     1441 gggttgagtt cgaaagtcat ccctccgcgt ctgttatttg cctgaatcat cctggcgatg
     1501 ctgcccgacg ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt
     1561 catcgcgggt ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg
     1621 attggacgcc gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg
     1681 ttgctatcgc acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg
     1741 tggccgtggt cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt
     1801 gcattgtctt gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg
     1861 gtgtggttgc cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat
     1921 gcgaaaactc gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg
     1981 ccatcttcca gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc
     2041 cagtctttcg ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact
     2101 tcgctgtcgt ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg
     2161 cctaatacag cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct
     2221 tctgctttca gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg
     2281 gtgttgaaat cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca
     2341 ggagcaacgg ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc
     2401 gcctgcttca ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg
     2461 aagtaacgca cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta
     2521 cccagcgatt tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag
     2581 ttaacatact gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg
     2641 aacatcaggt cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc
     2701 attgccgaac attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc
     2761 ggttcgccct ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg
     2821 accacgtcaa cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca
     2881 taagtcggat cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga
     2941 cctttggcga tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc
     3001 ggctccatat ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc
     3061 atgcgatcca ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg
     3121 tcgtcgatat cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag
     3181 cgcgcaacca cgtcaaaagc aacaaaggta cgcccgtgac caatatgaca gagatcgtaa
     3241 acggtgattc cacacacata catgccgact tccccggcgt gaataggctt aaattcctct
     3301 ttttggcgtg tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg
     3361 ggtaattgag ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat
     3421 gatccgatcg tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag
     3481 caccaatcaa acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat
     3541 tgtcatcaaa acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg
     3601 ccgcgaaggt ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca
     3661 gggcggcggt tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga
     3721 agccaacaac ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc
     3781 gcactctgca actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc
     3841 tggcgaaagc ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga
     3901 cgtggtagac aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc
     3961 aaaagaagac gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt
     4021 attgcagatc ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt
     4081 ttagcggggg aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg
     4141 tggattggcg acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg
     4201 gtgtccgatt ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc
     4261 aaacgctttg cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt
     4321 tatggccgcc gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat
     4381 caggcttttc gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg
     4441 ttgtttgtgc gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc
     4501 agtaaatcgc tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa
     4561 catcaggtgc aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc
     4621 gccaaccagc aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg
     4681 gtgaaagtca cggcggatga cgttgagctg attcattttc cgttttaaaa aacccgcaac
     4741 tttgctgatt tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt
     4801 gccctctaaa gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat
     4861 aatccggcgc gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc
     4921 gccgccgaaa tcttcgaaat cctgaatgtc ctgcaccacg ttgaagtggt ttctgctcac
     4981 cgcacccccg ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg
     5041 attattgcgg gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg
     5101 gtgccggtgc tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac
     5161 tccatcgtac aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc
     5221 gcggcaaacg cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac
     5281 cagcgtctga atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg
     5341 cgaggtacgg catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc
     5401 gtcaggcagg cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg
     5461 cggcggtgcc ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg
     5521 cattaacccg cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta
     5581 ttgctgaccg tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt
     5641 ggcagttact tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg
     5701 cgattgttaa gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca
     5761 atgaaaccga acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta
     5821 acttctctgg tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt
     5881 atacgctgac gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc
     5941 aggccaacgc acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc
     6001 tgggctatgt gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca
     6061 acgaactggc tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca
     6121 gccagtttga gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga
     6181 ataatccgtc ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat
     6241 tgccgctggt gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc
     6301 atctgaattt gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc
     6361 cgctgctgcc gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag
     6421 caggtgctca tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt
     6481 acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc
     6541 cggatgctct catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc
     6601 aattcgcgat aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac
     6661 cgccgttacc ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg
     6721 tgtggcatgg cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc
     6781 atcggtgagg atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag
     6841 atctttatca atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc
     6901 gccactgcaa atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag
     6961 aattttacgc ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat
     7021 ctgccagccg taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat
     7081 aaatttctct ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac
     7141 cgtcgtaaca ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat
     7201 catcccctgg ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc
     7261 cagattctgt aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg
     7321 ataagaacgg gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt
     7381 ttccgccgtc agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag
     7441 tgttttcatg gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct
     7501 gcccctgacg tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg
     7561 caactcagag catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa
     7621 cgtaaataac tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa
     7681 aactggcgta actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc
     7741 ccggtttcct ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc
     7801 gtaatacgca gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc
     7861 ccttgagcca caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat
     7921 tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt
     7981 aacagttcgc cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc
     8041 caggactggc gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt
     8101 gcgcgttcct caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca
     8161 cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc
     8221 gataccggta ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc
     8281 caggcacgcg ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta
     8341 aatttccgcc atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa
     8401 gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat
     8461 cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc
     8521 ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc
     8581 gaacatattg ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc
     8641 agcatccatc gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac
     8701 gctgaggaaa tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg
     8761 tggggccagc gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc
     8821 gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc
     8881 cagcgccgcg cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac
     8941 atcttcgccg taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta
     9001 cgcccggttg ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat
     9061 accgcccatt ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg
     9121 ctccagtatt gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc
     9181 cacgcacgcc cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg
     9241 aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag
     9301 ccagtgtgga cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga
     9361 tgtaaacatg ggaacccctt attgcaaacg ttctaataaa cgagccaggt aatgactcca
     9421 acttattgat ag
//
LOCUS       DAETQN010000155         9397 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.157, whole genome shotgun sequence.
ACCESSION   DAETQN010000155 DAETQN010000000
VERSION     DAETQN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.157"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(350..1603)
                     /locus_tag="K0W68_003809"
     CDS             complement(350..1603)
                     /locus_tag="K0W68_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3075251.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="K0W68_003810"
     CDS             1710..2603
                     /locus_tag="K0W68_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3075252.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="K0W68_003811"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="K0W68_003811"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBI3075253.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="K0W68_003812"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="K0W68_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBI3075254.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="K0W68_003813"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="K0W68_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBI3075255.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="K0W68_003814"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="K0W68_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBI3075256.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /locus_tag="K0W68_003815"
     CDS             complement(6839..7693)
                     /locus_tag="K0W68_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBI3075257.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="K0W68_003816"
     CDS             complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="K0W68_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBI3075258.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..>9397)
                     /locus_tag="K0W68_003817"
     CDS             complement(8323..>9397)
                     /locus_tag="K0W68_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBI3075259.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2449 a         2379 c         2438 g         2131 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccgcaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgg
//
LOCUS       DAETQN010000156         9212 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.158, whole genome shotgun sequence.
ACCESSION   DAETQN010000156 DAETQN010000000
VERSION     DAETQN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.158"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            73..732
                     /locus_tag="K0W68_003818"
     CDS             73..732
                     /locus_tag="K0W68_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBI3075260.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="K0W68_003819"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="K0W68_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBI3075261.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="K0W68_003820"
     CDS             complement(1860..2570)
                     /locus_tag="K0W68_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBI3075262.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="K0W68_003821"
     CDS             3004..3294
                     /locus_tag="K0W68_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HBI3075263.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="K0W68_003822"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="K0W68_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBI3075264.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="K0W68_003823"
     CDS             complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="K0W68_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBI3075265.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="K0W68_003824"
     CDS             complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="K0W68_003824"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBI3075266.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="K0W68_003825"
     CDS             complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="K0W68_003825"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBI3075267.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7538..7837)
                     /locus_tag="K0W68_003826"
     CDS             complement(7538..7837)
                     /locus_tag="K0W68_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBI3075268.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8012..9007
                     /gene="wecH"
                     /locus_tag="K0W68_003827"
     CDS             8012..9007
                     /gene="wecH"
                     /locus_tag="K0W68_003827"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBI3075269.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9049..>9212)
                     /locus_tag="K0W68_003828"
     CDS             complement(9049..>9212)
                     /locus_tag="K0W68_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075270.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         2286 a         2328 c         2268 g         2330 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagctgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atccacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gcacagaatc taagatccct gccatttggc ggggattttt
     3841 ttatttgttt ttcaggaaat aaataatcga tcgcgtaata aaatttagta ttatttttgt
     3901 gaagaataaa tttgggtgca atgagaatgc gcaacgccgt aagaaaggcg ggaataactt
     3961 cccgccaaag actcttactc tttcaaattg caggctaaaa atgccgccag ctcataactc
     4021 tcctgtttaa tatgcaattc acacagtgaa tctcttatca tccaggtgaa aaataaaagc
     4081 gtgaaacaaa tcactattaa agaaagtaat ctatatttct gcggcattcc cagcctcctg
     4141 tgttgatttc caacgagtaa gaggctaacc ttatggtgtt caggcatagg gtagcctcac
     4201 tttgatttat agtcaggtgg ggcttttctc tgtctgcctt tcggtgaata cctgagacaa
     4261 acagtctcaa gcacccgtgg ctattctagc ttaataagtt tgtttcttct ccttgatata
     4321 atcctaaaaa aacctcataa aattaatata tgagataatc tttattcaac agaagattat
     4381 taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta
     4441 ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag
     4501 tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac
     4561 tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg
     4621 gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc
     4681 cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc
     4741 gtcagatttc gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc
     4801 atccagggta cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt
     4861 cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca
     4921 ggcgcggaag cgaccgagca taaagtcgat aacatcatca actacgttgg cgttagtcag
     4981 cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt
     5041 cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt
     5101 gtcgcctttc ggatgctgac cgataccgaa gatacccgcc agagtatcca tcttatcagc
     5161 aatcgccagc gcacaagcta ccgggttgga cggcaggtca tcaccggcaa agcgcggctg
     5221 atactgctca ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg
     5281 catccccata acgccttggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca
     5341 cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat
     5401 ccagccagcc agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg
     5461 gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt
     5521 gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat
     5581 ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc
     5641 atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc
     5701 agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac
     5761 cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg
     5821 cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac
     5881 tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg
     5941 ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatggttgc
     6001 cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac
     6061 gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagcg aagtcgcaac
     6121 catattcggc agtagtgctt cagtgctttc gcccttcaca tgggcgcgat acagcagcca
     6181 ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
     6241 ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg
     6301 gccgcgtttt tcgatttcac gatctggttg cgcttccgcc aggttagcta ctttcaacgc
     6361 cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag
     6421 ctccgcagta aagttcgcag caaaggactc agccaggctg cgcagtgctt ttggtggcag
     6481 ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc
     6541 tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc
     6601 tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcagtgacgg agatggcttt
     6661 acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag
     6721 cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact gctcgaagca
     6781 ggtgaacagg aaatccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt
     6841 ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agaccaggtc
     6901 gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc
     6961 gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga actgcgtcac
     7021 ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag tcgggttttc
     7081 ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca gctctttcag
     7141 agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg
     7201 atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga
     7261 aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg
     7321 gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg
     7381 agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg
     7441 catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga
     7501 tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa
     7561 aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta taaccgcgca
     7621 tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt
     7681 tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat
     7741 caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaccaaccc actagcacca
     7801 taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg
     7861 tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct
     7921 ataagctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga tttatcggtc
     7981 agactttttt taaccagata cagagacaca gatgcagccc aaaatttact ggattgataa
     8041 cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct ggtatgtgac
     8101 caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga attctgcctc
     8161 tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg gcgaacgcag
     8221 cgcccagccg cgccatttct tgcgtatcgg cttatgtctg attttttata gcgcaatcgc
     8281 actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga aaaacctgct
     8341 gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga tttatctggt
     8401 ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc taatggcggt
     8461 gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt ttgaatggct
     8521 gccaattaac ttatatatca atggcgatac tttttactac attctgtatg gcatgttggg
     8581 ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga gcgccgcgct
     8641 gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc agtggcgcgg
     8701 aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct gcgcaatcgc
     8761 gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac ttggcttaat
     8821 ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg cgctgcgcac
     8881 ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct tttgcgcgac
     8941 gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa acagattagt
     9001 gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt attcgctgta
     9061 ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggcatcac gaatattctt
     9121 ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa aacctttttc
     9181 actgagtaat aatgtcgcat tccacagacc ta
//
LOCUS       DAETQN010000157         9186 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.159, whole genome shotgun sequence.
ACCESSION   DAETQN010000157 DAETQN010000000
VERSION     DAETQN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.159"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            172..1770
                     /gene="rtcR"
                     /locus_tag="K0W68_003829"
                     /pseudo
     CDS             172..1770
                     /gene="rtcR"
                     /locus_tag="K0W68_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1752..2510)
                     /locus_tag="K0W68_003830"
     CDS             complement(1752..2510)
                     /locus_tag="K0W68_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBI3075271.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2527..3357)
                     /gene="glpG"
                     /locus_tag="K0W68_003831"
     CDS             complement(2527..3357)
                     /gene="glpG"
                     /locus_tag="K0W68_003831"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBI3075272.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3402..3728)
                     /gene="glpE"
                     /locus_tag="K0W68_003832"
     CDS             complement(3402..3728)
                     /gene="glpE"
                     /locus_tag="K0W68_003832"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBI3075273.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3918..5423
                     /gene="glpD"
                     /locus_tag="K0W68_003833"
     CDS             3918..5423
                     /gene="glpD"
                     /locus_tag="K0W68_003833"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBI3075274.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5475..6080)
                     /locus_tag="K0W68_003834"
     CDS             complement(5475..6080)
                     /locus_tag="K0W68_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075275.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6083..6850)
                     /locus_tag="K0W68_003835"
     CDS             complement(6083..6850)
                     /locus_tag="K0W68_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075276.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6853..7563)
                     /locus_tag="K0W68_003836"
     CDS             complement(6853..7563)
                     /locus_tag="K0W68_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075277.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7579..9084)
                     /locus_tag="K0W68_003837"
     CDS             complement(7579..9084)
                     /locus_tag="K0W68_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBI3075278.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2372 a         2358 c         2261 g         2195 t
ORIGIN      
        1 gttgtttgct gtcgtgataa ttgcaaccgt cgtgccagaa aattaaataa gcagattaat
       61 tttttaattc attgttttta aataggttaa atctttacgt ccgtaaacgg agattttcca
      121 caaaaccaat tcaccgataa tgaaatatcg tcttttataa ggatatctaa gatgcgtaaa
      181 acagtggctt ttggctttgt cggtaccgtg ctggattatg ccggacgcgg cagtcagcgc
      241 tggtcgaaat ggcgtccgac gctttgttta tgccagcaag aatcgttagt catcgatcgg
      301 ctggaattgt tgcacgacac tcgctcgcgc tcgctattcg aaacgcttaa acgcgatatc
      361 gccagcgttt cgccagaaac agaagtcgtg ggcgttgaga ttgaactgca taacccgtgg
      421 gatttcgaag aggtttacgc ctgcctgcat gatttcgccc gtggttacga gtttcagcca
      481 gaaaaagaag actatttaat tcacatcacc accggcaccc acgtcgcgca gatttgctgg
      541 tttctgctgg cggaagcgcg ttacctgccc gcccggctga tacaatcttc acctccgcgc
      601 aaaaaagaac agccgcgcgg cgcaggtgaa gtgacgatta tcgatctcga tttaagccgt
      661 tataacgcca tcgccagccg ctttgccgag gaacgccagc aaacgcttga ttttcttaag
      721 tccggcattg ccacgcgtaa cccccacttc aaccgcatga ttgagcagat cgaaaaagtg
      781 gcgatcaaat cccgcgcgcc gattctgctt aacggtccaa ccggcgcggg caagtcattt
      841 ctggcgcgac gcatcttcga gttaaaacag gcgcggcatc agtttagcgg cgcgtttgtg
      901 gaagtgaact gcgccaccct gcgcggcgat accgccatgt cgacgctgtt tggtcatgta
      961 aaaggcgcgt ttaccggggc gcggtaatct cgtgaaggtt tattacgcag cgccaacggc
     1021 ggaatgttgt ttcttgatga gattggcgaa ctgggcgcag acgaacaggc aatgctgcta
     1081 aaagccattg aagagaaaac cttttacccg tttggcagcg atcgccaggt gagcagtgat
     1141 tttcagctta tcgccggaac ggtgcgcgat ttgcgccagc tggttgccga aggcaaattt
     1201 cgcgaagatc tgtacgcgcg gatcaatctc tggaccttca ccctgccggg tctacgccag
     1261 cgccaggaag atattgaacc gaaccttgat tatgaagtgg agcgccacgc cacactcact
     1321 ggcgacagcg tgcgttttaa caccgaagcg cggcgcgcct ggttggcttt tgcgacctct
     1381 ccccaggcaa catggcgcgg taactttcgc gaactttctg ccagcgtcac gcggatggcc
     1441 acctttgcca ctagcggacg catcactctg gacgtggttg aagatgagat aaaccgtctg
     1501 cgctataact ggcaggagag ccgcccctcc gcgcttacgg cgttgctggg cgcagaggca
     1561 gaaaacatca atctcttcga ccgtatgcaa ctggaacacg ttatcgctat ctgccgctag
     1621 gcaaagtcgc tttctgcagc cggacgacag ctttttgatg tttcgcgcca gggcaaagcc
     1681 agcgtcaatg acgcggatcg gctacgcaaa tacctggcgc gttttggtct gacgtgggaa
     1741 gccgtgcagg atcagcacag ctccagttga atatgatggt ccgtcagcac ctgcattacg
     1801 cttactggcg gcggggcgtc ggtgtagacg gcatctacca tgctgatgct gcccatattg
     1861 accatcgcgt tacggccaaa tttcgagtga tcgacaacca gcataacgtg gcgcgagttc
     1921 tcaataatgg cgcgcttggt gcgaacttcg tggtaatcga actccagcag cgagccgtcg
     1981 ctatcgatgc cgcttatccc cagaatgccg aaatcaaggc ggaactggga gataaaatcg
     2041 agcgtcgctt cgccaatgat cccgccatcg cggctgcgta attcgccacc ggcgagaata
     2101 atgcggaaat cttcttttac catcaacgtg ttagcaacat tgagattgtt ggtgacaata
     2161 cgcaaattgc tgtgattaag cagcgcgtgc gccaccgctt ccggcgtggt gccgatatcg
     2221 ataaacagtg tcgagccgtt ggggatttgc tccgccactt tgcgggcaat cctctctttt
     2281 tcttcggtct gggtggcttt gcgatcgtgc cacggcgtgt taaccgaact ggaaggcagc
     2341 gccgcaccgc catggtgacg caggatcagg ttttgctccg ccagctcatt gaggtcgcgg
     2401 cgaatagtct gcgggctgac ggagaaatgc tctaccagct cttcagtact gacataaccc
     2461 tgctgtttaa ccagttcgat aataccgttg tgacgttgtg tttgtttcat ttataaatcc
     2521 ctggaattat tttcgttttc gcgcattgag cgaatcaaca aaagccatcg ctaaacccac
     2581 ggctaacccg gcgatgtgtg ctccgttcgc catcgacatc ccaaacaaat caaaccatcc
     2641 ggcgacaatc cagatcagcg caaagataat taacccacgt tgcaggtaaa tgccactttg
     2701 cggatcgcgt tcaccacgta gccagacgta gcccatcagc gcatacacca cgccagaaag
     2761 cccgccaaac cacggcccgc tgaatttttg ctgcacatag ccgcttaaca gggcgctaat
     2821 gagagtaata acaattagct taccgctacc gaggcgtttt tccaccgcac cgccgagata
     2881 ccaccaccag agcaggttaa agaggatatg catcagcgag aagtgcatta acgcgtgggt
     2941 gaagtaacgc cagaactcaa atttcagtgc tggatcgaat ggccaggcca gccataacat
     3001 cacttcctga tcgccgagaa tttgcatggc aataaacacc accacgcagg cgatcatcat
     3061 cacccaggtc accggacctg cgcgttcacg caaggcggca aagaaaggat aacggcgata
     3121 atgcaggcca ctgccggtat ggcctgcctg ccagctggcc gccagataac gcggatctgc
     3181 cgggttttcg agaaaacgcg ccagctccgc ccgtacgcgc tcggcctggg actcatccgc
     3241 cagccagaca tcgctttggt tatgttgttg aatcgtgagg ataacaccct gcgtcgccat
     3301 gtaatcaaca aacgcctgcg ccacgcgggg gttagcaaaa gaggtaatca tcaacatcgt
     3361 tgctgtcgct tattccacac aaaaggggac agtataaagc gttacgcgcc gtacgccacc
     3421 tctgcgggaa actgacgttg ccaggcttca aagccgccgt caatgctata gaccacatcg
     3481 tagccctgtt gcagcagata ctgcgccgcg cctttgctgc tattgccgtg ataacacatc
     3541 accatcaccg gagtgtcaaa gtcgttatca cgcataaacg cgcccagcgt gtcgttggtt
     3601 aaatggaaag cctgcaccgc atgccccata gcgaaactct gtggatcgcg aatatcgacc
     3661 agcaccgcct ctttttcctg caacttctgg tgcgcgtcgg caacgttaat acattcgaac
     3721 tgatccatgc gtctctcttt ctttacaaac aagtgggcaa atttaccgca cagtttacgt
     3781 cgaagcggca gataaacgcc ataatgttat acatatcact ctaaaatgtt ttttcaatgt
     3841 tacctaaagc gcgattcttt gctaatatgt tcgataacga acatttatga gctttaacga
     3901 aagtgaatga gggcagcatg gaaaccaaag atctgattgt gataggtggc ggcatcaatg
     3961 gtgctggtat cgcggcagac gccgctggac gcggtttatc cgtgctgatg ctggaggcgc
     4021 aggatctcgc ttgcgcgacc tcttccgcca gttcaaaact cattcacggt ggcctgcgct
     4081 accttgagca ctatgaattc cgcctggtca gcgaggcgct ggctgaacgt gaagtgctgc
     4141 tgaaaatggc tccgcatatc gccttcccga tgcgttttcg cctgccacat cgtccgcatc
     4201 tgcgcccggc gtggatgatt cgcattggtc tgtttatgta cgatcatctg ggtaaacgca
     4261 ccagcctgcc gggatcaact ggtttgcgtt ttggcgcaaa ttcagtgtta aaaccggaaa
     4321 ttaagcgcgg attcgaatat tctgactgtt gggtagacga cgcccgtctg gtactcgcca
     4381 acgcccagat ggtggtgcgt aaaggcggcg aagtgcttac tcggactcgc gccacctctg
     4441 ctcgccgcga aaacggcctg tggattgtgg aagcggaaga tatcgatacc ggcaaaaaat
     4501 atagctggca agcgcgcggc ttggttaacg ccaccggccc gtgggtgaaa cagttcttcg
     4561 acgacaggat gcatctgcct tcgccttatg gcattcgcct gatcaaaggc agccatattg
     4621 tggtgccgcg cgtgcatacc caaaagcagg cctacattct gcaaaacgaa gataaacgca
     4681 ttgtgttcgt gatcccgtgg atggacgagt tttccatcat cggcaccacc gatgtggaat
     4741 acaaaggcga tccgaaagcg gtgaagattg aagagagtga aatcaattac ctgctgaaag
     4801 tgtataacac gcactttaaa aagcagttaa gccgtgacga tatcgtctgg acctactccg
     4861 gtgtgcgtcc gctgtgtgat gatgagtccg actcgccgca ggctattacc cgtgattaca
     4921 cccttgatat ccatgatgaa aacggcaaag caccgctgct gtcggtattc ggcggtaagt
     4981 tgaccaccta ccgcaaactg gcggaacatg cgctggaaaa actgacgccg tattatcagg
     5041 gcattggccc ggcatggacg aaagagagcg tgctgccggg tggtgccatt gaaggcgacc
     5101 gcgacgatta cgcagctcgt ctgcgccgtc gctatccatt cctgactgaa tcgctggcgc
     5161 gtcattacgc acgcacttac ggcagcaaca gcgagctgct gctcggcaat gcgggggcga
     5221 taagcgacct cggggaggat ttcggtcatg agttctacga agcggaactg aaatacctgg
     5281 tggatcacga atgggtccgc cgcaccgacg atgccctttg gcgtcgcaca aaacaaggca
     5341 tgtggctgaa tgcggatcaa caatctcgtg tgagtcagtg gctggtggaa tatacgcagc
     5401 agaagttgtc gctggcgtcg taaggatgat gcccggttga agtgcaaccg ggctatttgg
     5461 attccttaga ctattcattg taaataattg aataacggcc cctgagctaa aatcgccaaa
     5521 cttaagcacg ttacccaatc gccccacttc ttcctctggt ttctattttt tctgcaccaa
     5581 tggcaataat atcccaatgc aaaaccaagc tgtgcactaa tcgggccgac aaaatcagtc
     5641 caaggctgcg gccaaaaagg ggaaaaattc aggggaggcc agacaaacgt cagaaagaaa
     5701 tttcggatat tttccagcaa cgctgatttc tctggagaaa tataaagaat gaaattcgag
     5761 agaattaacc agaaatagac cggcgaaaag accagccagc aatgcagtct gttttttaat
     5821 tcaggaggcg ttctgcggga gaattgccaa ccgatgccag ccattacact gaaaaaaagt
     5881 gcatattcaa ccattaactc aagttgtaat tcctgaagag aaatacgcac aaccaggagc
     5941 ggaaaggaga atattatttc tgccagaaaa gttaacgtga atataagtac ggctaagaca
     6001 ataaccgtcg gccataccaa taatggcacg ataacaaaac cgagaattac agggggaata
     6061 taaagccgga atttaatcat ttttattttc ctggtgattc ggatgatgcg tcatacatcg
     6121 cgcataaaca ctcatcaatt atccagtcat cttcccagac ctgatattct gtcattggtt
     6181 cgggctgttt tagcgggttc tcaatcgctt gccgcgctct ttctttcagt aaaggatgat
     6241 tatttctagc ccaggcattg atttgggtgg taatggcgtt atcgatatcg tcctggttat
     6301 ctctgatata atcctcacaa tatctgttca ataaacaggc aagagaaacg gtcgaccaaa
     6361 agccgggaac tgaattaatg gcatccagca atggcgcaag agcgatatcg gcacggaaaa
     6421 acatttccag catatactgt aattcgacag cttcatcgcc ggatacccaa tcagtcatat
     6481 actggcaggc aaatcgctgt aaaattgccc gttcatccgc acgccagttt tctttgttgg
     6541 caaggttgat acggcgcaga gaaaaaacta cgtgaatatt gtcaaaatgc ccctgtgcaa
     6601 caaattccag taaacgcggc agaaaatagc ggacctcatc gctattttgc ggatcaggcc
     6661 ctgggctact attccaggca ttgattaagg aatacgggat cgctcgtaac gatgtactcc
     6721 gtaatgcctt ttgttcctgc tgactcaggc aatattcaca acaaaccaca aatttctgcg
     6781 gcacccgata acgacgaaag gtgtgataaa tctgctcaat taatgcctgc atggcggggc
     6841 tgggttgcat agttaccccc tcgcccgctg atcgcgtgta aacggttgat agacaaaacg
     6901 agcattcgcc gcgttcaggc gaataagggc gctacgcgaa agggaaaagc tggggcgaat
     6961 gcaactattg cagtctcgac agctttcctc tgcgcaattt tccactgagt aatgacgcgt
     7021 gtagagtttc agttcggcct gcttatcgcg aaatccgcgt aaactgttat tgttatgaaa
     7081 tttccaggca tctttaaaga cctgaaagat atagacatcc tcaatccagg agtcacattt
     7141 tttaagcggg cgctttgcta ttatcacccg ctcatccgga tttagcatcg ccgtaaattg
     7201 tacggtgacg tcatttgtcg tgttcaggtc acattgaaca tctggtacat cactgatata
     7261 ggggtcaaat ccaaaatcag caccacaggc acaaagacgg ctaatggtgg cggtatcgag
     7321 ggaaaatccc ggccatacct tgctggaggt taagccaata tttaatgtga catcatatcg
     7381 aagcaatccc tcaagaccgt cagcaccgac gaacttttcc ccttcatcca aggcctggtt
     7441 aataagttgc tgaacaatgc cttcatccca gtatgccgat ttgcagttgg caatcgtaat
     7501 atccacgcct gtgaagggat cagtaccggc aaggcttgca aataatgtat taactaattc
     7561 catttttatt atccagcttt aaggtagcgt cttatacatc gggacataac ctacatcttc
     7621 caccaaactc tgtccctgcg gcgttaaaaa ccactccaac aatttttgtg tctctgacgt
     7681 cgtattttcc cgcgttacca taaaggcatc gacgatatag ggatatttgc cgttgcgaat
     7741 gttttcggca gtcggtgcaa taccgttaat cgctagcagt tttatatttt tatcggcgtt
     7801 catttgtgtt gcgtaataac ggaaggtgta acctattgcg ttgtttgtat tacggtattc
     7861 ggcaacgact ttaatcattc cctccatcat acttgccact tctgtttcct gcggcgagat
     7921 catgcggaca tttttcatga cctgggattg catcaccgtc tggctaccag agtcttccgg
     7981 gcgttgccag gtctggatct cctgatcgtt accgccaaca gtacgccagt tggtaattgc
     8041 accactgaag atgtcacgca cctgttgttc agtcagggaa ttaaccggat tatccgcatt
     8101 gacgatgaaa acaaacgctt cacgggcaaa aggcgtgtac agcaaagtaa cgcccgattc
     8161 ctcagcgcgt tttttctgcc caccggaagg ttgcgccaca aaaataatat cagcatcacc
     8221 tttaacaatc ctgttatacg catcgggggt acgagagctc tccagatatt ccctggtgtg
     8281 aaaatcttcc ggtataacgc ttaaagcata aaatgcagag gcataaattg ggtacgccgc
     8341 cgtagcacca tcgagtcgcg gccagttttg cgtaaactga atttgcggtt taccacgcag
     8401 tggtgtcagt tgattattga gtttatcggg tcgccaggcc caggtatcaa tattttcgcg
     8461 cacaccgaca ccgggattga gctgtttata taaataaccc tgataaccag cgacaaaacc
     8521 aagcatgaga aggataaaca ggagggaatt acggcaacgt agcccggatg taccggttaa
     8581 aggatgtctg gttggccggc cgtaccccag cgaaaaactg atatgcgcgc caacgggtat
     8641 aatgactatc caccagtact cacctccgac tactgagact aacaaaacgc ccagccaggg
     8701 gagcataaga tcagccaacc aacccgatcc catctggtga aattgataat ctgccagatg
     8761 cagcgcgatt atccagccaa ccaggattaa taaaccgggt actaaaaaaa gacattgtcg
     8821 ccagacggct atttctcttt tttctggtgc actatcgcct gcccatactc gccccgagaa
     8881 aaatagcatt aatgcgccca gcgccagcat aaaaatgttg agcagaaggt aacttgttgg
     8941 cgtgataagg atattacaaa cttcaataat accggcaagt ccgacaccca gcatagcaac
     9001 aaccgccgcc aaaaattgcg tcagtatggg gatgccgaaa aaagtcatta ccagcgagga
     9061 caaaatccat tttctgtttt gcattattct ttccattctt tttgaatggt gaaattatac
     9121 tccccgagtc cccttgcccc ttctggacac ttttccgaaa tgatggcgga aaaaaacggg
     9181 accctt
//
LOCUS       DAETQN010000158         9072 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.160, whole genome shotgun sequence.
ACCESSION   DAETQN010000158 DAETQN010000000
VERSION     DAETQN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.160"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(506..1273)
                     /gene="fecE"
                     /locus_tag="K0W68_003838"
     CDS             complement(506..1273)
                     /gene="fecE"
                     /locus_tag="K0W68_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HBI3075279.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="K0W68_003839"
     CDS             complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="K0W68_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HBI3075280.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="K0W68_003840"
     CDS             complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="K0W68_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HBI3075281.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="K0W68_003841"
     CDS             complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="K0W68_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HBI3075282.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="K0W68_003842"
     CDS             complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="K0W68_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HBI3075283.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="K0W68_003843"
     CDS             complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="K0W68_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HBI3075284.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="K0W68_003844"
     CDS             complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="K0W68_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HBI3075285.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8343..9040
                     /locus_tag="K0W68_003845"
                     /pseudo
     CDS             8343..9040
                     /locus_tag="K0W68_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1987 a         2508 c         2585 g         1992 t
ORIGIN      
        1 cactttttag aaaaatcgat ggtcagaaac tggattagca attccgttcc agggttgctt
       61 ttgatttacg ttggcgtctg atcattgatt tatcctcaaa agcccaacct cattggtaat
      121 gaaccagctc cgtgaatgtc cgctctggca cagagcgaaa ttttttgatc tcccccccct
      181 gaaatctaaa cttagtcatg tcacgttttt gggtttctaa aattttaact tcgcgttttt
      241 cgttgccgta agggttatac agaaatgtcc gttaagcaga gttcaaaatt gattgccgtg
      301 atcacgactg gtttgaaagc cgcgcccaag cctgtacagc tctggtttgc gttgattatg
      361 aacctgtcag cctaaagcaa gcggatggac gatgagtatt ggtaatcttt cagagtccgg
      421 aaaagttcag ccccagtctg aacaggcttg ctggcgccag tccagtttca ttcagtcgtg
      481 gtttggttct tacggcctgt gcaatctacc tcattaggca catcggcctg ccagataccg
      541 gctcggggtg tatttccgct tccacgctga atactgttct cagcaatcct ggggtcatca
      601 cctcttctgg tgtgccttgc gccataacat gtccgtttgc cattaccacc agttgatcgc
      661 agtaccggct agcctgatta aggtcgtgca gcacagcgac caccgttttc ccctgagtcc
      721 ggagttcgcc catcaaccgc atcaggtcca cctggtgatt gatatcaaga taggtggttg
      781 gctcatcaag taatacaacg ggcgtattct gggccaggac cattgccaga aatgcgcgct
      841 ggcgctgacc gccggaaagc tcggttaacc gacgaacggc aagatgattg atccgggtct
      901 ggttcatggc gacattaact cgtgcattgt cttcagcgga gagacgcccc cagagtgaca
      961 gccagggatt acgaccatac gaaaccagct cctggactgt gatcccctct ggcgttaaat
     1021 ggtgctgagg cagcagcgaa agcctgcggg ccaactggcg cgatgagagc atatttatgg
     1081 gattatcgcc gagaaatacg gtgccagact gcggcattaa aagccgcgaa aaacagttta
     1141 acagcgtcga tttcccgcaa ccgttaggac cgatcagggc ggtgatcttc cccgttggca
     1201 gtgagagtga aacgtcgtta agtaccttgt ctgttccgta attgaccgtc agattttcag
     1261 ttcgtaaagt catttatcgc attctcacaa gcaaccagac aaaccacggc gcaccgataa
     1321 tggcggtcag cacgccaacc gggagctcca gtgggggatg aataattctc gccagcagat
     1381 cggcaaccac caacagcaac gcacctgtca gggccgaaac aggcagcagt ctgcggtgac
     1441 gtccaccggt gatgctacgc atcatatgcg gcaccacgag accaataaag ctaatcgggc
     1501 cgcaggcggc cacgccggta gatgtcatgg cgacagctag taacaaagcc cagaatcggg
     1561 tatagggcac cgacacaccg agcgtggtgg cgcgcgcatc gccgagtgca aggaggtcga
     1621 gatcgcggca aaaactcagg ctcagcggca gaaataaaat catcagcggg atggcaatct
     1681 tcacaaagct ccagtcacgg ccccataagc tgccggtcag ccacagcagg gcgttgttca
     1741 catcctgcgg gcgcgagagc atcagataat ccgtcaggct ggcccagcat gcagaaagcg
     1801 ccacgccggt gagtgccagc ttcatcggct ggtgggtctt tgccagcatc ttcagcaata
     1861 tcaaccccgc catgccgccc gcaaaggcca gcagcggcag caccatcacg ggcagtgacg
     1921 gcataagaag tagagccccc acagaggcca ggctggcggc atggttaacg ccgagaatat
     1981 ccggtgatgc cagagggttg cgcacaatcc cctgtatcag cacgcccgcc acggcgaggg
     2041 ctgcaccgac aaacagtgcc agcagcaagc gcggcagtcg gtactccatc aatacataat
     2101 aatgctcgcg tccggcctgc cagtcggtca gcagcgcgcg ccacggcacg gggatcactc
     2161 ccatatggag tgataacagc gcacagcccg ccagggcaag ggtgatgaaa ataaccagcg
     2221 caattttcat cctcgcctcc tcacaagcca gacaaagcaa gggctgccaa tcagcgccag
     2281 cactgcgcct gcgggcagat ctccggggaa ggccagcgcg cgtgcgagta catctgccag
     2341 cagcatcagc gtggccccca gcagcatgct cactggcagt acgttgcgct gatcgaagcc
     2401 tgcccagaag cgcgccagat gtggcaccag cagaccgata aacgccaccg gacctgccac
     2461 gctgacgcac gcaccaacca gaagcagcac taacatattg atgaccaaac gtagcctcgt
     2521 caggttcact cccagcgtat gggcggtgct gtcgctgagg ttgagcaggt tcagttgatt
     2581 cgccagcagc aacacgacag ggactgcagt gaccaccacc ggcaagagct gccagacatc
     2641 ctgccagcgg gcgtgggaca ctccgccagc cagccagtaa aagatgccgt aagcatgatc
     2701 ttcggccagc agcagggtga tgcgggtaag gcccatacaa aaggccgaca gcgcgatacc
     2761 cgcgaggatc agtttgtttc tgtcatgggt atgacgaaat ccgcctcctg cggtcatgac
     2821 cagcagccag ctcacgccgc ccccgcatgc cgcgatgaac gacagagaat agcctgcaat
     2881 cggcgtcgga ctcagcgcgc tggtaagcgc catagccagc gccgcgccgc tgttaatgcc
     2941 gagcagtgaa ggagaggcca ttgggttgtg ggtcagggtt tgcagcagcg tgcccgcgag
     3001 cgccaggctt gcgccgatca gaacggcgac caggcttcgt ggcaaacgaa ggttttgcac
     3061 cagcgcttct ggtagcgttg gcgtgtgtcc aggcagcagg gcgcgggttg catctgctcc
     3121 ggaaacagga atggccgagt agcaaaacag actcagccag aaaataataa taagtgctgc
     3181 aacgggaagc ccccacagca gcaccgggtg ttttatcgcg gtcatttcac aacggtaagc
     3241 ggctgatggt ggaagatttt taccgtgtca gcggcaatac gctctgcagc aaaaataccg
     3301 cgcatccgcg cccaggtgtt actgtcgacc gaagcaacct gctgcttctg cgcggcggtt
     3361 aacatctgcc agagcggatc ttgttgccag cgtttaacaa tgctctcttc gcgatagtgg
     3421 gcaaccagca gccaggcagg attgaccgcc agcagttgct ccaggccgat ggacggcatg
     3481 gacgcacccg ccatcgcagc gggaacgttc agccccagag aggccagcac gctgccggtc
     3541 caggtctcct gagtatgcag gttgaattgc tgttcgcgtg atgtgccaaa ggccacgcgt
     3601 gtccctttgg gaagctggct ggcccactgc gccatcctct ctttatgttg ttccagacgt
     3661 gcctgcatct ctcgcttttt acccaccatt tcgccgatga tagccgcaga ttgcaaattt
     3721 tcagcgtagg tttcgttgcg ggacttaagc agcagtaccg acgcgatttg ctgcaaggcg
     3781 atgtaaaccc ccgcatggcg actgctgtcg gcaatgatca ggtctggttt cagagcggca
     3841 atggcttcca ggctcggctg cgcgcgcgtt ccgacggact gccacggttt caggtgcgca
     3901 cgcacttcgg gcaggatgcg ttttgcatcg ttatcgtcgg caataccgat cgggctgacg
     3961 tccacggcgg ccagcgcatc ggcgaacgag agttccagca ccacaatccg ttgtggcgtt
     4021 ttttcgagtg taaacgtgcc gtgttcgtcc tgaaccgtgg cggcaaaggc gtggctgatc
     4081 accagcagca ggcctgcaaa aagaaaacgg ataaatgcca acataatcac attccagcta
     4141 aaagcccggc aagccgggcg ttaacacatc agaacttcaa cgccccctgc atatacagcg
     4201 tgcgcggctg gcctgcatag atgcctttgt tgttgtcgtc ataagagcgg atgaagtagt
     4261 cctggtcgaa gatgtttttc acaccgaatg ccaggttcag atctgccatc tgcgggccaa
     4321 agtcatacac cacgcgtgcg ccccagagca tgaagccggg aatgcggccg gtactgccgt
     4381 cggcgctctc tttcaccgta ttggcgttat ccgcaaactg gctggactgg aaatcgctgt
     4441 tcagattgaa catccagttt cctggcttgt agtccacgcc cagcgtgcct ttatgtttcg
     4501 gggagaatgg taccagattg ccgtaggtgt cgcctttctc gcggatttcc gcgttcacat
     4561 acgcatagct ggcgtagatg gaaacgttat caagcgttgg cgttagcgta cccagatcgt
     4621 aacgtgcctg cgtttccagc ccggtatggc gcgttttgcc acgtgcagtg acggtgtcgt
     4681 tggtctggtt ggagtcgtac tgattgttaa agttaatcag gaacagcccc atttccgccg
     4741 tcagcgcgcc gtcgtcgtag cgggtaccga gttcccaggt tcgcgctttt tccggttcca
     4801 cattgccgct ttgcacagcc ttgccaatct ggctgtactg tacggtgccg aacgagcctt
     4861 cagtgtttgc ataaagattc cagctgtcag tcaggtgata gagcacgttc aacgccggaa
     4921 gcggtgcgtt atagctcact tcttcgtgcg tgcctgtgat ggcgttgttc tggtatgact
     4981 cgatatgttc gaaacgcata cccggcgtga tggtccagtt gccgatgtcg attttgtcat
     5041 ccagatacca ggcgtgcgcc tcggtgccgg aacgcgtatc gcggtcgtaa gggcttgagc
     5101 cggacggcaa ctgcccgctg ctggtggcgg tgtagtaacg catttcatgc gttgattcat
     5161 tcacatagcg atagcccacg cccacttcgt gcgcggaagg gccgatcata aagatctggc
     5221 tgtagcgtgg ctcaataccg cgcacccagt agttacgcgg cgagagggtg atgcgtttgc
     5281 cttgctccag gtagccgctg cgcagggttt gggtgtagaa cccctgaatg ttgaatttat
     5341 gctggctgtc tggctggaac tggtagccca ggctcgccag cttgcgacga ccccagaagc
     5401 ggtcatacgg gcgggtggat tgccagcgat cggcgtcgta atccgcgcga gacaggccac
     5461 cgggcatgtc ggcttcaccg tcgtaatatt gcagcaggct gttgaaggtg tgcacctcat
     5521 ccggcgcata tttgcttttc agcatcaggt cgtcgatgcg ggtggcgctg tgctcgcgcc
     5581 agtcactgcc gcgcgtgccg gagtagagca gcgcggtgcc aaaaccgttg tccgctgtgc
     5641 cgcccaccat caggttgtgc gtctctttcg ggttgttttg tgaagaggtt gggctgagct
     5701 gaccttccac gcccgcctcg ataccaaagt cctgcggaat ggcacgggta acaaagttca
     5761 ccacgccgcc cacgctctgc ggtccgtaac gcaccgcacc accaccgcgt accacgtcaa
     5821 tggcattcat gttgccgagc gaaacgggag ccagtgaaag ctgcggctga ccgtaagggg
     5881 cgaaggggac ggggatgccg tccatcagga cggtcgagcg gctggcgagg cgcgggttca
     5941 ggccccggat gccaaagttc atcgccaggt cgtggctgcc ggtgccgttg ttttccggcg
     6001 cgctgacgcc agggatgcgg ttaagtacct cacgcatggt ggttgcgccg gttttggcga
     6061 aatcctcacg gcggatcacg tcacgcgcgc cagcatgttc aaatacgtcg ttttcacgcg
     6121 catcacccag ccagtcgccg accacggtca gggcatcttc ttttggtgcg ggcgcgggct
     6181 ccagcgtcca gctgttattt cccagcggtt ttacctgcag tccgctgccg tccagcagtt
     6241 gttgcaggcc gctctcgacg tcgtaatcgc cgtgcaggcc gttgctctgc ttgccgcgcg
     6301 tcaggctggc gtcaaccgag agggtaaatc cgctgtgtgc ggcatactga ttgagcgctt
     6361 tatcgagcga tcccggtgcg atattaacct gtgcagcaaa agcggaaaac gagagaccgg
     6421 ccagcggcag caggctcagg cgaatggcgt taaccaaagg tgttgtttta cgaaaaacgc
     6481 gtaacggcgt cataccttcc ccatcatcat ttttgttgtg ttcagctatg agtcgaacga
     6541 gaagggtaaa aaggacaatc gaaataagaa ttattttcct tacagtggtg aaatgtttat
     6601 ccagtaccgc gtaatagact gaattttaac gggaagcgtt tgcgcgataa cgttcaggat
     6661 cgcatcggta tttttcagcg ggaacgtccc gctcaggcgc agcccggcaa cggcgggatc
     6721 gcagcgcagc acgccgttgc ggtaacgggt tagcgtggct atcacctcac ccagcggttt
     6781 atcgctgaag ctcaggatgt ccttcgtcca gcttgtactc tcgtcatcca gcggtttcac
     6841 tgcgccaaac tcagaggcgc tgaactgcag gctttcacca gcgttcacga tgcgtttttg
     6901 cgcgggggca ctggcgagaa gcacttccac ggcgtgctgc tgcacgtcaa gctgcgtgaa
     6961 attatcctgc tggcggacgg taaattctgt ccctaaagca gtgagctggc cctgacgggt
     7021 caggacacgg aaggggcgtt gcagggcatc tttcgcggta gtaatggcga tttcaccgta
     7081 ccagagccgg acggtgcgct gatgcgcatc aaaacgcata tccgccgcgc tttgggtatt
     7141 cagcgtgagc agggagccat cttccagttg ctgacggctg actgtgcctt tggcggtgcg
     7201 gtaatctgcc cgcagacctt cgccggtttc cgactgccag agctgccagc ctccgccagc
     7261 gccgagcaac agcagtaatc ctttcatcac gtgacggcgg gtgaggcggg tatcgtgcaa
     7321 cgcccggctg gcaacgtcgc caggcacacc gccaagctgg ttgcgcaggt tttcaacctg
     7381 ctgccaggcc cactggttat cctgatcctg ttcataccac tgttgccagc gcgcttcctg
     7441 ttgtgggctg acgcgctcgc cgcttagcac ggcataccag tgggaagctg aacgcagcgc
     7501 ctgacggcgg gaatcggtta acaaaggatt cataacccat actccagacg gaacagcagg
     7561 cagtgctcga cggctttcgc cacgtatttt ttcacggagc tgatggaaac accgagtttg
     7621 tgcgcaatct cgctgtatgt cagaccatcc agttgcgaaa gcagaaacgc ttcacgtgtt
     7681 ttgccgttta gcccgtccag catgctgtcg aggagttgta gggtctcgag ttggctttcg
     7741 cgttcctcag gtgaaggcgc tcccccctcc ggcataagcg ccagcatctc cagatacgct
     7801 ttttccagcg cgtttcggcg aaacaggtcc accatcacgc gtttggcgat agtgcagagg
     7861 aaggagcgag gatcgcggat cgtcgagagc gtttcgctga ccattacccg caaaaaagtg
     7921 tcctgggcaa tgtcatctgc atcaaaagca gactggagtt tgcgcgtcag ccagcttttc
     7981 aaccagccgt gatgtgtgcc ataaagcgac tcgaatgtta aggaagctgt ggtagtggcg
     8041 cggtcagaca tgcggagtgc atcaaaagtt aattatcacg tagccatatt aatatgagaa
     8101 tagttatcat tacaattgga aataaaattg tttccaatag acatttttaa catgttgttt
     8161 ttctaagtgt tataaggtag gtataaaatg ggatggagcc tctgcttctg gcatgtgtcg
     8221 gtcagaatga ctcatgatgt ggtctgctat tattgacatc ctcactgccc taaaggatag
     8281 ggatttcggt aatgctgtca acttactgat ttagtgtatg atggtgattt taaggtgctt
     8341 gcgtggcttc catttccatc agatgtcctt cctgctccgc tactgaaggc gtggtgcgta
     8401 acggcaaaag cactgccgga catcagcgct atctctgctc tcattgccgt aaaacatggc
     8461 aactacagtt cacttacacc gcctctcagc ccggtaagca ccagaaaatc attgatatgg
     8521 ccatgaatgg cgtcggatgt cgcgccagtg cacgcattat gggcgttggc ctcaacacgg
     8581 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac aaccgggcag
     8641 tgatgtgatt gtctgcgctg aaatggacga acagtggggc tacgtcggtg ctaaatcacg
     8701 tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg cgcacgtctt
     8761 cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg cctttgaggt
     8821 cgtggtatag atgacggatg gctggccgct gtatgaatca cgcctgaagg gaaagctgca
     8881 cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc tgagacaaca
     8941 tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc tgcatgacaa
     9001 ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca ttacctcctc
     9061 ccgaactcaa aa
//
LOCUS       DAETQN010000159         8838 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.161, whole genome shotgun sequence.
ACCESSION   DAETQN010000159 DAETQN010000000
VERSION     DAETQN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.161"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            812..1054
                     /locus_tag="K0W68_003846"
     CDS             812..1054
                     /locus_tag="K0W68_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075286.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="K0W68_003847"
     CDS             complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="K0W68_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBI3075287.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="K0W68_003848"
     CDS             complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="K0W68_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBI3075288.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="K0W68_003849"
     CDS             complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="K0W68_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBI3075289.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="K0W68_003850"
     CDS             complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="K0W68_003850"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6320..6322),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBI3075290.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7101..7934
                     /gene="fdhD"
                     /locus_tag="K0W68_003851"
     CDS             7101..7934
                     /gene="fdhD"
                     /locus_tag="K0W68_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBI3075291.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            8087..>8838
                     /locus_tag="K0W68_003852"
     CDS             8087..>8838
                     /locus_tag="K0W68_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HBI3075292.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQE"
BASE COUNT         2121 a         2232 c         2265 g         2220 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcagta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat
     1321 ggtgtcgtca caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag
     1381 agattactct ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc
     1441 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
     1501 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
     1561 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca
     1621 gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta
     1681 acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag agccacatac
     1741 cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc
     1801 catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1861 ggacgagaaa tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc
     1921 cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc
     1981 aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     2041 cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     2101 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     2161 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     2221 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     2281 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     2341 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc
     2401 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     2461 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     2521 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc
     2581 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     2641 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     2701 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2761 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2821 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2881 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2941 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     3001 tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag
     3061 ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     3121 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg
     3181 taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct
     3241 cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     3301 ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca
     3361 gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     3421 ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg
     3481 catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     3541 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     3601 agcgcatcac cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt
     3661 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3721 agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct
     3781 gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag
     3841 ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta aactccggcg
     3901 tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag ccttttttag
     3961 ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg
     4021 ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg
     4081 aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga
     4141 tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt
     4201 ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca
     4261 atgcttcggc gtcgtcttta aagattcgcg cagccgggtt cgagataacg tttggatgca
     4321 gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg
     4381 ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga
     4441 cgccgctgcc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc
     4501 cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg
     4561 cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac
     4621 cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc
     4681 taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg
     4741 agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg
     4801 ccagcgcctt accgttgctc tccatcgcca cttcttccga cttcggctca tgcggaatgg
     4861 cgtagttcca ggtcatgttc agcacctgat ccgggtttgc gccaccctgt tcggcataca
     4921 tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccctg
     4981 gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt
     5041 tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt
     5101 caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt
     5161 cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga
     5221 atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca
     5281 tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc
     5341 agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt
     5401 tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg
     5461 taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc
     5521 tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt
     5581 taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac
     5641 gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag
     5701 ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg
     5761 gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca
     5821 agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt
     5881 ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga
     5941 acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt
     6001 cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga
     6061 aagcaatgtc agtaccggaa cgaatagggg catagtagtc agccaccgcc gccgtacgcg
     6121 taaagcgagg atcgatcaca atcagcttcg cgccattgtg aattttggct tccatcgccc
     6181 agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg
     6241 cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
     6301 ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg
     6361 cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca
     6421 tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag
     6481 catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt
     6541 gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt
     6601 ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat
     6661 ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc
     6721 tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg
     6781 tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg
     6841 ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga
     6901 cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg
     6961 tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc
     7021 tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt
     7081 tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa
     7141 atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt
     7201 tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg
     7261 tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg
     7321 ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc
     7381 ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg
     7441 cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa
     7501 aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat
     7561 tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg
     7621 cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg
     7681 cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg
     7741 tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag
     7801 agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta
     7861 atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc
     7921 gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg acttattcat
     7981 ttcgtgaatt ttattatttt atttataaat aaaacgcgtg tagtgacgtt ttcttttttc
     8041 atttattatg ttcactctct tatctatata tttataaggc aattaaatga aaaggaattt
     8101 attatcttcc gctattatag tcgccatcat ggccctcggt ctgacgggtt gtgatgataa
     8161 aaaagccgaa acagaaacgc ccccgcctgc caatagtcaa cctgccgcac cagctcctga
     8221 agctcccgtt gcaaaagcag aagctaaacc tgaaacacct gcgcaaccgg tggtcgatga
     8281 acaagcggtt ttcgacgaaa aaatggatgt ctatatcaag tgctacaaca agttacagat
     8341 ccctgtacag aacagtctgg cgcgttatgc tgactggctg aaagatttta aacaggggcc
     8401 taccggtaaa gagagtactg tttatggcat ctacggcatt agtgaatcca acctcgctga
     8461 gtgtgaaaaa ggcgtaaaaa gtgctgtggc gttaacgcct gcgctgcaac ctattgatgg
     8521 cgtggcggtg agttatattg atgctgccgt ggcgttgggt aataccatta acgaaatgga
     8581 taaatattac acccaggaaa attataaaga cgatgcgttt gcgaaaggga aaacgctgca
     8641 ccagacattc ttaaaaaatc tggaatcctt tgaacctgta gcagaatctt atcatgcggc
     8701 gattcaggaa attaatgata agcgccagct tgcggaactg aaaaatattg aagaaagaga
     8761 agggaaaaca ttccactact actctctggc agtcatgatt tcagcgaaac aaattaataa
     8821 cctgatttcg caagagaa
//
LOCUS       DAETQN010000160         8633 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.162, whole genome shotgun sequence.
ACCESSION   DAETQN010000160 DAETQN010000000
VERSION     DAETQN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.162"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="K0W68_003853"
     CDS             complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="K0W68_003853"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBI3075293.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="K0W68_003854"
     CDS             complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="K0W68_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBI3075294.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4609..5766
                     /gene="mdoC"
                     /locus_tag="K0W68_003855"
     CDS             4609..5766
                     /gene="mdoC"
                     /locus_tag="K0W68_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBI3075295.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="K0W68_003856"
     CDS             complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="K0W68_003856"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HBI3075296.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="K0W68_003857"
     CDS             complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="K0W68_003857"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBI3075297.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7825..8136)
                     /locus_tag="K0W68_003858"
     CDS             complement(7825..8136)
                     /locus_tag="K0W68_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI3075298.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="K0W68_003859"
     CDS             complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="K0W68_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBI3075299.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2156 a         2247 c         2051 g         2179 t
ORIGIN      
        1 ataacgccgg tatttttatt ggctcgttgt ttttgccgga tgcgacgtga acgctttacc
       61 taacccacaa aagcttgcaa aatcaataaa ttgcatgaac gatgtaggcc tgataagcgt
      121 agcgcatcag gcaactacat ttttattgcg aagccgcatc cggtttacgc aatgccagtg
      181 gattgagctt tatcccttcg taataactca cccatgaaga atatctctca ggggaattcc
      241 agacgcggaa atgcagacgg gccatcgtca ccggatcgct tagcagcaca aggcgacgat
      301 cgcgattcag cttctctggc gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga
      361 tttccagcac cttactggcg cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa
      421 atgacgggtt aaacactgcg tgcataaagc catcgtcaag ggagcgttgg cgattcatct
      481 caaggaaccg atcggtatca accagcacct gcggcggtga atactcttcc gggatcagga
      541 acagtttcca gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa
      601 acggtgacag gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca
      661 gccacgccat cccaacagcc cacactaacc ctaacagcag ctgtgagccg tggcgtttga
      721 acgcttcacc ccaggaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc
      781 agccaaggaa cgcgctgacg acgaagaccg tatggaacag catgcgtacc ggagccagca
      841 gcacggaaaa cagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccgtatt
      901 ctttcgttcc tttgcaccag ataagcaaaa tgctcaataa cttcggcagg aacaacagca
      961 ccatggtcga agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga
     1021 acaactgccg tggttgcagg aagtattgcg gttcggtcaa cgcatgtact acctgcaatg
     1081 cagtagagag cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg
     1141 tcaggaacac cgcacggtga accgggtgca tacccttcac caggaacaga cggaagttca
     1201 tcaggttacc gtggcaccat cggcggtcac gttttagctc atcaagcaag ttaggcggca
     1261 attcttcata agaacccggg agatcgtaag caatccagac cccccaacct gcacggcgca
     1321 tcaacgccgc ttccacgaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg
     1381 gcagcggagc cagtgcgcag tgctcgataa acggtttcac gcggataatc gcgttatgtc
     1441 cccagtagtg cgactcgcca agttgccaga agtgcaaacc ggctgtaaac agtggcccat
     1501 acacgcgggt cgcgaactgc tgacagcgcg catacagcgt atccataccg gacgctttcg
     1561 gcgacgactg aatgatcccg gcgttcgggt tggcttccat caggcgcacc agcccgcaca
     1621 aacaatcacc ggtcattacc gagtcagcat ccagcaccac catgtagctg tactggctgc
     1681 cccagcgacg gcagaagtca tcgatattac cgcttttacg cttcacgcgg cgacggcggc
     1741 ggcgatagaa aatctgacct tcgccaccga cttcagcgat aagctccatc caggcttttt
     1801 gctctgcgac gcagatatcc gggttataac tgtcactaag aatgtagaca tcaaagtgtt
     1861 tggcattccc ggtggctttt actgattccc acgttgcacg caggccagca aaaacacggt
     1921 tcacgtcttc gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt
     1981 catcgccaac tgttgacgca gatatactgt atttatcgcg accaataagc agttgcagga
     2041 agcccattaa cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaagagga
     2101 tcaggatacc ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct
     2161 gaccaaccat atccatagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca
     2221 tataccaggt cgcgacgaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga
     2281 tggtaccgac ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca
     2341 gatagcgcgg cgtgacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg
     2401 tacgccacgg gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttcagctgat
     2461 cgcgcccttc gtcgtcttta attaactgtc catcagcaag tgaatctggc caggcttgtt
     2521 ccagacgcgc ctttaccgag ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt
     2581 cggcatccag cgcctgatga acggcgcgga tatcagtctt cggcaatgcc gctttctcgc
     2641 ttgcggcgat gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg
     2701 tagctccagg tttcactcaa cgtctgatcg gcattcacca gcgcagcacg catttcagtg
     2761 gttttcttgg catctttcac tttcacacgc atcaccagac gccagccttt ggtaaccggg
     2821 ttatagcgca ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg
     2881 accggggtat cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg
     2941 atagtaccgt caggctggcg aatcaggttc gactgcttca catcccccgt tgaacgacgc
     3001 gtttgttgca cccatgcgtt atctggcgca tgcagtttgt cttcatcacg gctgaaggtg
     3061 atggtgtatt taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa
     3121 gcgacgatgt tatcgttggt ttcatcgttg gttggaattt ccaccagctc aacgctgcct
     3181 ttaccccact cccctttcgg agtcacccat gcgcttggac gaagatcgta acgatcatcg
     3241 agatcttcaa agcgggagaa atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttt
     3301 tccatcgaga agctgctaac cgctaaatgt ttcgggttat tcaacggacg ccagatccat
     3361 tcgccattac cggcatggat agagagaccg ttagagtcgt gcaactccgg acgatagtta
     3421 tttgcaggcg acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc
     3481 agtttgccga ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc
     3541 cctggcataa ccacgaattt gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa
     3601 atggttaaac gtttatcagt cggttttgga cgctcgatcc agaactcttt gaagcgtgga
     3661 aattcttccc ccgatggcaa ggcggtatca attgccaggc cgcgggcaga aaggccataa
     3721 acctgacctg caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg
     3781 tttttatctt tgctgttgat cgggtaaagc actttaaaac cggcaaaacc aaggtctttt
     3841 accgtgtctt tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt
     3901 cgtttgactg cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacataccc
     3961 tgatggtaga actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga
     4021 ttaaactgga tctgctgata gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg
     4081 cttttgggcg cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca
     4141 atactgaaag cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa
     4201 cgcattttca tcatttgtag tttatgtttc ataattagta aagcacttcc ccctttgtgt
     4261 gcttatatcg atccgatcta ttttaatgga taaccagcca ttccgacaac agaatcccgg
     4321 tatcgttcag cacgctggct catggattaa gcacctgatt gcaaccgttt tcactttccg
     4381 tgcttctcct aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa ttttatttat
     4441 caaccgtctt acagacgctt ccattctggt tagtggtagc gatgatgcta tgcataaaaa
     4501 ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcgct atggcaacaa gcctaaaggc
     4561 ataacccgac agggataaga cgaaaaatcc gagattagtt aaccatatat gaatccagta
     4621 cccgcgcaac gtgaatattt cctcgactcc atccgcgcct ggctgatgtt gttagggatc
     4681 ccttttcata tttctttaat ctattcgagc catacatggc atgtgaatag cgccgaaccg
     4741 tcattgtggc tgaccctttt taatgacttc atccactcgt tccgcatgca ggtatttttc
     4801 gttatatccg gctacttttc ctacatgctt tttttacgct atcccttgaa aaaatggtgg
     4861 aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag ccatcccctt actgacattg
     4921 ccgcaattta ttatgctgca atacgtcaaa gggaaagcgg aaagttggcc tgggctgtca
     4981 ttgtatgaca aatataatac gttggcctgg gaattaatat cacacctgtg gtttttactg
     5041 gtgttagtag tcatgacgac gctgtgcgta tggatattta aacgcatcag aaataattta
     5101 gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa aactatcggt gattttttta
     5161 tgcctcggca tcggctatgc ggtaataaga agaacgattt ttattgtgta tccgcccatt
     5221 ctgagtaatg gcatgttcaa ttttattgtc atgcaaacgc tattttattt gccgttcttt
     5281 atcctcggtg cactggcttt cattttccct catcttaaag ccttgtttac cacgccgtct
     5341 cgtggctgta cccttgcagc agcattggca tttgtcgctt atttactcaa ccagcgctat
     5401 ggcagtggcg atgcctggat gtacgaaacc gagtcggtga tcaccatggt cctcggtctg
     5461 tggatggtga atgtggtctt ctcctttggc caccgtttgc ttaacttcca gtcagcgcgg
     5521 gtgacttact ttgttaacgc atcgctattt atctatctgg ttcaccaccc gttaacgctg
     5581 tttttcggcg catacattac accgcacatc acctccaact ggcttggttt tctctgtggc
     5641 ctgatattcg tagtagggat tgcgataatt ctgtatgaaa ttcatttacg catcccgtta
     5701 ctgaagtttt tgttttctgg taaaccggtt gttaagcgtg agaacgataa agcaccagcc
     5761 cgttaagcca catttacaat aaccattcca cgggcaatat cgacgccagt ctgaccataa
     5821 cccgcttcca gaaactggtg gcgggttctt ttttgagaat aatctctttc tttgcatgac
     5881 gatcaaccca gttgatccgt ccccacctgt ccagacggag ctgccaggcc gcatcatact
     5941 ggctctgaat aaagcgttta tcaattaact gtgccagcgt ttcgctctct atcacgaagc
     6001 ccatttcagt attgagcaat gttgaacgcg gatcgaaatt gaaagagccg ataaacaccg
     6061 ttttaccatc gatgctaaaa gttttagcat gcaggctggc tccggaatta ccggttatgc
     6121 cgcgatcgtg taacgtacta ctttgttcac gcgtcggctt gagttcatat aattccacgc
     6181 catagcggag caattttttg cgccagcgcg cgtatccggc atggacgaca gcaacatcgt
     6241 tagcggcaag agaattggtt aggatcgcaa tctttacccc ttttctcacc atccgtaaga
     6301 gttgcgccac acctgcgcgt gtcggtacaa aataggaaga gataatatcg atgcgttcac
     6361 tgggtgagcc catgatatcg gacaggcgct gcggtaacag tgaatgccgt tttgccttgc
     6421 cctccccttt cgccggatca tcacttaata aacgtgtttt cgcccagata agcggcaacg
     6481 ttccatcaac cagatgattt ataaacggac tggattccat ttggcgtaag taacgatgcg
     6541 tcatggcatc gttatgccag gaggcgggta actcgatgcg atccgccatt tcaccttccg
     6601 ggacatccag cacctgctgt aagggtgaaa ccgatttgca ataccagtag cgggcgaaat
     6661 catcggcaac gtcctctacc acgggtccta ttgccataac atctaaatcc gaaaaaagtg
     6721 gctcttcccc tgccccaaaa taggcatcac caatatttcg tcctcccacc agggtaacca
     6781 cgccatcgac agtgaaactt ttattgtgca tacggcgatt gagacgggaa aagtcggtga
     6841 tataaccaag cggacgcagc aagcgaaacg agaaaggatt aaaaagccgg acttcaatgc
     6901 ggggatggct gtcaagcaag cgtaaaatat cgtcaagtcc gggcgtattg ttgtcgtcca
     6961 gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa cagggcggaa aacagtaacc
     7021 gacccgacat atcgtcctgc cagatgtaat actgaacatc cagcgtatgc tctgccattt
     7081 cggccagacg ataccgggcg gcaaacgcat ccagactttt ctccagaggg aaaagaccac
     7141 actgaccggg atgttgcgaa cacagtggca gcaccgcgct cgccagccgg ggcaaatcat
     7201 tcatctcctt gttgggtaag gagtctttcg tagaggtggg cgttttcttc atcataacag
     7261 acaaagtata cctgttcggg taaagcgtga cgggtaataa attctgaaac ggttttaact
     7321 gcgatttcag ccgctgccgc acgagggtaa ccataaaccc cagtactgat tgcaggaaaa
     7381 gccactgacg tatagctgtt tgccgccacc agtcgcaggc tattcagata ggcatcctgc
     7441 aaaagctggt cttcgttttg ttcaccacca cgccagactg gcccgacggt gtgtactacg
     7501 gctttagcgg gaagatcgcc tgcaagcgta ataacggcat gccccgtagg gcaatcgccc
     7561 tgctgttgcc tgacttttaa acaggcatcc agcagggccg gacccgctgc gcgatgaatg
     7621 gccccatcga cgccgccgcc tcccattaat gacggattag ccgcattcac aatcacatca
     7681 acggccagtt tggtaatatc accctgcaca acatgaatac gcgttttcat aacgcctcct
     7741 tacaccttct tgtttcttaa gtgtacagca ggctttttgg ttaaatcgca tttttctgct
     7801 cagcaacgtg cgttgttcag aaacttagtt atattcgatg ttcattacag ccagcttttt
     7861 ctgtttatca agatactgca cttttactga ggctatttgc gcgtttttaa cattaccgct
     7921 cattaacgct ttgctggaat aggttttgcc cggaacagaa ccattctgtg ggcagttcat
     7981 ttctacgggt gatgactggg tgctgacgtc acatgccggt tccacaattt ggccataaaa
     8041 atggatcaca cccccttgcg cggtgcttcc tgcgatggca ataaaaggaa aaaacaaact
     8101 gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct caatgattag
     8161 tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag cagaccattc
     8221 tctccagatt catcttatgc tcgatatttc aacaaattaa gacttttctg aagagggcgg
     8281 ccattgttgt gataaatgaa gtgcctgtcc atcagaaaca gtaacaacta ttttcacccg
     8341 atcttccggg gaaatattta aactcaactt cgtcaaagca ataggttgat tagcaggcaa
     8401 tgagagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa
     8461 tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa tggtatacac
     8521 atccccttgc tgggtcgtat taaaggttat ctggctggaa agtgccgcaa ggagtaataa
     8581 cgcattcata ttcttctccc gaaaaaaaca gggcttgcgc cctgtttctg taa
//
LOCUS       DAETQN010000161         8533 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.163, whole genome shotgun sequence.
ACCESSION   DAETQN010000161 DAETQN010000000
VERSION     DAETQN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.163"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="K0W68_003860"
     CDS             complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="K0W68_003860"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HBI3075300.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHS"
     gene            complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="K0W68_003861"
     CDS             complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="K0W68_003861"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HBI3075301.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(1322..2986)
                     /locus_tag="K0W68_003862"
     CDS             complement(1322..2986)
                     /locus_tag="K0W68_003862"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HBI3075302.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            3028..3861
                     /gene="mhpR"
                     /locus_tag="K0W68_003863"
                     /pseudo
     CDS             3028..3861
                     /gene="mhpR"
                     /locus_tag="K0W68_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
     gene            3938..5020
                     /gene="lacI"
                     /locus_tag="K0W68_003864"
     CDS             3938..5020
                     /gene="lacI"
                     /locus_tag="K0W68_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HBI3075303.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            5143..8217
                     /gene="lacZ"
                     /locus_tag="K0W68_003865"
     CDS             5143..8217
                     /gene="lacZ"
                     /locus_tag="K0W68_003865"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HBI3075304.1"
                     /translation="MIMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPHWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            8269..>8533
                     /locus_tag="K0W68_003866"
     CDS             8269..>8533
                     /locus_tag="K0W68_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3075305.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         1941 a         2390 c         2334 g         1868 t
ORIGIN      
        1 aactatggcc gcccatcgag ttgcccagca ggtggatttt ggcgatatcc agttgatcca
       61 ccacgctttt caggattcgt gcattaagat ccgatcgtga accactatta acgatcgaat
      121 cgcttttgcc ccaacccgga caatccagca ggatcacccg atagcccgcc tctaccagcg
      181 gatcgatatt gcggctgaag ttcgcccagc cagtagcacc cgggccggaa ccatgcagca
      241 ggacaacggt ttcgtcgcct tgtccgcagt cattaaaatg gatgcgcagc gttttacccg
      301 cttcttctac attcagaaaa cggctggtgg cggcttcggt ttgtggctga taactcatca
      361 tcttctcctg catattcagt tctctgttct ggcgcttaac gagccaaatc cggcaatcca
      421 ctccgggatt gggcggtaat aacgcccttc gctacgccag ttgccaaatg cagaaatagc
      481 ggcaaaagcg gcgacccagg ttttgatttc atgtgtcgac tttccggcaa tggcggaaag
      541 ctcttcgtta ctgacggcat ccagttcctg tatgcgtccc tgctccagca aagtcatgaa
      601 ctggttatcc caaatcgggt tgagcggatg cagagttctc tgatcctcaa caaacttctc
      661 agcggcgcta atcacccttt gctgacgcaa ttcgcgctca ctggcgggta aatctttccc
      721 gctccccaac agacggtcgc gcatatgggc atcggctttc gccagttcgg gcaccggcgg
      781 ctgatgggaa agcccaccgg aacccaggaa cagcacgcgt ttattgagag tgctggtgaa
      841 acgtccaatg gcttcaccca acatgcgggt acgctggaaa ccgggcagcg gcgtggcgac
      901 accgttgatg aacacaggca gaactggcac cttatccagc ccaccgagca ggaactccag
      961 cggctgggcg aacccgtggt ccacctgcat acagtaagaa acggcaagat cgatcccgct
     1021 cttcatgaca gcatgcgcac aggcctccgc cagctccaca ggcacgggca gctctcctgc
     1081 cgcactgccg aaatcaccaa ttgccgtcgc tccaacgcct aaacagaacg gtggcatcac
     1141 gtcatagaaa aagccgttgt agtgatctgg cgcaaacagc accaccagtt caggggagaa
     1201 agccgcaata cgctcgcggg cgctggcaat cacgccattg acctcatcga gtacctcttg
     1261 cgccgggtcg acatacccca ccaacggcga gtgggaaaga cagtgaagat aagcgtgcat
     1321 atcaggctac cttttcgaca gaaacgtcgg catcagggcg ggtcagcgtc atcaccgacg
     1381 ccagtttatt cagcgtattg cccagggttt gcggaatggc ggtggcggca acaaagcgat
     1441 ccgggcgcat caccaccagc gaagcattgt gttgcgcgaa ccagctacgc agacgacctt
     1501 gcgtatcgcc cacgcgtagc acgccgtcgt ggttatcctg tgcggtatga atttgcactt
     1561 ccggcaccac ctgaatgaag cgtgtgccca acgcgcgcca ctgctggatt tgctcgtcgc
     1621 tcatccccca cagtggattg catccccagc caattaccgc gaagttcgcg ccgatcgcgt
     1681 tatcgagcag cgtcacgtcg ccgttttcca gcgtgacttt cggctgaata aacatcttgc
     1741 cgaccggaga gtgcttcgct tcgccctcac gcaccagcgc accgccgtaa tattgcggca
     1801 tcggcttgaa gcgcatttcg aggaagtagc gttttactgg cggcagataa ttcaacagcc
     1861 aggaaacgcc gtcacgtaac gtaccctgcc agcgtttcgg cggagccagc acgttgcccg
     1921 ccgtcacgga caggtcaatc atcgctttgg cgtgatcgcg acgttcttgt tgataggtat
     1981 cgagcagcgc atcgcgggct ttcccctgga taaccaacgc cagtttccag gcgaggttaa
     2041 aggcgtcgcg cataccactg ttatagccct gcccctgcca caccggcatg atgtgcgcgg
     2101 catcgcccgc cagcagtacg cgatcaatac ggaaacgttg cgccagtcgc gcgttatggg
     2161 tgtagacacg ctggcgaatc aattcaacat tgtccggatt aggcagcact ttgcttaaca
     2221 gcttgcgcat attttgcggc tcacgcagct gctcttcggt ttctcccggc atcaccataa
     2281 attcaaagcg acgtaccgca tgaggcagcg cggcagaaac atacgggcgc accggatcgc
     2341 aacacaaata gatatgcggc gtacttaacg gatcgttggc gatatctacc acaatccact
     2401 gatttggcgc agttttacct tcaaacggca cattcagggt gcgacgaaca aaacttgccc
     2461 caccatcaca ggccaccagc cactgggctt tgatcgtttc ccgctgccct tctgccgttt
     2521 tcaggtgcaa ggtcacttcg tcatcttgct ggctgaaggc ctccagctcg cgggaaaaca
     2581 agcagcgcac attcggaaaa cgcgacaacc cttccagcat caccgcatcg acctgtggct
     2641 gaataaaggc gttacggcgc ggccagccaa attcatcggt cattggctga atatcagcaa
     2701 aacagcggcc ttttggggtg agaaaacgca tcgcgtgcca cggtgtagtg tgcggcagaa
     2761 cattctcgac caggccgacc gactgcatgg tgcgcagcgc ctcgtcatca ataccaatcg
     2821 cacgcgggta gtcgatcaac ttatcgagtt tctccaccac cagcacgtca atgcccatct
     2881 gaccgagata gttcgccatc atcagcccaa ccggaccggc accagcgatc gccacctgaa
     2941 cgctatggtt aacagcaggc tggatgtcag ggtgttgtat tgccatttct gcacaacgtt
     3001 gttttaacta tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc
     3061 gtgcgcggct taacccgcgg tctaatgtta ttaaatatgt taaataaact tgatggcggt
     3121 gccagcgtcg ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg
     3181 ctggagacgc tgcaggaaga gggatatgtc cgccgtagcc cctccgatga cagttttcgt
     3241 ctgaccatca aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca
     3301 ctggcggccc cgctgctggg cgatctgttg cgcgaagtgg tatgaccaac agatgtgtcc
     3361 accctggatg ttgatgcaat ggtggtacgc gaaaccaccc accgtttcag ccgcttatcc
     3421 tttcaccggg caatggtcgg gcgacgttta ccgctcctga aaaccgcctc gggcctgacc
     3481 tggctggcct tttgcccgga acaagaacgc aaggaattaa tcgaaatgtt agccgcccgc
     3541 cccggtgatg actatcaact ggcacgagaa ccgttaaagc tgcaagccat tctggcgcgc
     3601 gcgcgcaaag agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc
     3661 tctatcgccg taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat
     3721 atggcgagcg caatgaccat tgaacaggca gcggaaaagc atcttccggc gctacaacgg
     3781 gtagcaaaac agatcgaaga aggggttgaa tcgcaggcta ttctggtggc cggacggcga
     3841 agcggcgtgc atttacgttg acaccatcga atggcgcaaa acctttcgtg gtatggcatg
     3901 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgctatacga
     3961 tgtcgcagag tatgccggtg tctcttatca gaccgtttct cgcgtggtga accaggccag
     4021 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     4081 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     4141 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     4201 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
     4261 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
     4321 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
     4381 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
     4441 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
     4501 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
     4561 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
     4621 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
     4681 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
     4741 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac
     4801 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
     4861 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     4921 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     5041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     5101 gtgaaattgt gagcggataa caatttcaca caggaaacag ctatgatcat gattacggat
     5161 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5221 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5281 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
     5341 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc
     5401 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat
     5461 cccattacgg tcaatccgcc ttttgttccc gcggagaatc cgacgggttg ttactcgctc
     5521 acatttaata ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggt
     5581 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagc
     5641 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggaaaaaa ccgcctcgcg
     5701 gtgatggtgc tgcgctggag tgacggtagt tatctggaag atcaggatat gtggcgaatg
     5761 agcggcattt tccgtgacgt ctcgttgctg cacaaaccga gcacacaaat cagcgatttc
     5821 catgttgcca ctcactttaa tgatgatttc agccgcgcgg tactggaggc agaagttcag
     5881 atgtacggcg agctgcgcga tgagctgcgg gtgacggttt ctttgtggca gggtgaaacg
     5941 caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat
     6001 gccgatcgcg tcacgctacg tctgaacgtc gaaaacccgg cgctgtggag cgccgaaatc
     6061 ccgaatctct atcgtgcggt ggttgaactg cacaccgacg acggcacgct gattgaagca
     6121 gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac
     6181 ggcaagccgt tgctgattcg cggcgttaac cgtcacgagc atcatcctct gcatggtcag
     6241 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac
     6301 gccgtgcgtt gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgcttc
     6361 ggcttgtacg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
     6421 ctgaccgatg atccgcactg gttacccgcg atgagcgaac gcgtaacgcg aatggtgcag
     6481 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgagtc aggccacggc
     6541 gctaatcacg acgcactcta tcgctggatc aaatctgtcg atccatcccg cccggtgcag
     6601 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc
     6661 gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg
     6721 ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt
     6781 cttggcggct tcgctaaata ctggcaggcg tttcgtcagt acccccgttt acagggcggc
     6841 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaatgg caatccgtgg
     6901 tcggcttacg gcggtgattt tggcgataca ccgaacgatc gccagttctg catgaacggt
     6961 ctggtctttg ccgaccgcac gccgcatcca gcgctgacag aagcaaaaca ccagcagcag
     7021 tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt
     7081 catagcgata acgagcttct gcactggtcg gtggcgttgg atggcaagcc gctggcaagc
     7141 ggtgaaatgc ctctggatgt tgctccacaa gataaacagt tgattgaact gcctgaacta
     7201 ccgcagccgg agagcgccgg acaactctgg cttacggtac acgtagtgca accgaacgcg
     7261 accgcatggt cagaagccgg acacatcagc gcctggcagc agtggcgtct ggcggaaaac
     7321 ctcagcgtga cactccccgc cgcgccccac gccatcccgc aactgaccac cagcgaaacg
     7381 gatttttgca tcgagctggg taataaacgt tggcaattta accgccagtc aggctttctt
     7441 tcacagatgt ggattggcgc tgaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc
     7501 cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga cccgaacgcc
     7561 tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc
     7621 acggcagata cacttgccga cgcggtgctg attacgaccg ctcacgcgtg gcagcatcag
     7681 ggaaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg
     7741 attaccgttg atgttgaagt agcgagcgat acaccgcatc cggctcggat tggcctgacc
     7801 tgccagctgg cgcaggtagc agaacgggta aactggctcg gattagggcc gcaagaaaac
     7861 tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg
     7921 tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat
     7981 tatggcccac accagtggcg cggcgacttc cagttcaata tcagtcgcta cagccaacaa
     8041 caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat
     8101 atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtttcagcg
     8161 gaattccagc ttagcgccgg tagctaccat taccagttgg tctggtgtca aaaataataa
     8221 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta
     8281 aaaaacacaa acttttggat gttcggttta ttctttttct tttacttttt tatcatgggg
     8341 gcctacttcc cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat
     8401 acgggtatta tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt
     8461 ctgctttctg acaaactcgg gctgcgcaaa tacctgctgt ggattattac cggcatgtta
     8521 gtgatgtttg cgc
//
LOCUS       DAETQN010000162         8443 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.164, whole genome shotgun sequence.
ACCESSION   DAETQN010000162 DAETQN010000000
VERSION     DAETQN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.164"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..400)
                     /locus_tag="K0W68_003867"
                     /pseudo
     CDS             complement(1..400)
                     /locus_tag="K0W68_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(586..3894)
                     /gene="icsA"
                     /locus_tag="K0W68_003868"
                     /gene_synonym="virG"
     CDS             complement(586..3894)
                     /gene="icsA"
                     /locus_tag="K0W68_003868"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HBI3075306.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGNGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVVGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQNEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4423..5625
                     /gene="virA"
                     /locus_tag="K0W68_003869"
     CDS             4423..5625
                     /gene="virA"
                     /locus_tag="K0W68_003869"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HBI3075307.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASNSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5804..6491
                     /locus_tag="K0W68_003870"
                     /pseudo
     CDS             5804..6491
                     /locus_tag="K0W68_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    6084..6200
                     /locus_tag="K0W68_003870"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            6493..6859
                     /locus_tag="K0W68_003871"
                     /pseudo
     CDS             6493..6859
                     /locus_tag="K0W68_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6787..7260
                     /locus_tag="K0W68_003872"
                     /pseudo
     CDS             6787..7260
                     /locus_tag="K0W68_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            8013..8378
                     /locus_tag="K0W68_003873"
     CDS             8013..8378
                     /locus_tag="K0W68_003873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075308.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATIPAGISADG
                     MYCPECGTVHWPDGVIPPF"
BASE COUNT         2457 a         1937 c         1710 g         2339 t
ORIGIN      
        1 gttcagttca ggctgtacgc ttccccatgt aatttgcctc gccacttcac cgggctcagg
       61 accccgtagg atcttcaccg aaaaaaatgc atcaccagtt gctttattat ttgttttagg
      121 tagacctgtt actaatttat acctactctg accaagtctg gataagaaag gcacacctgc
      181 acatttctac ttggaaatgt tttcttttac cattttgctc aaaaatttca acatccaata
      241 ttcctcgtac agaactactc aagtcctgaa tagaaaaagt tccagctggt acagtagagt
      301 catatattac tcgaccttgt tgagatacaa caacacgtgc attagtttct gcaataccag
      361 taatctgagg cgcataacca cgaatatttg gcggtagcat tcgttcatcg ctttctaatg
      421 caattcctgt gtaacgccaa gcaacagcgc attctggcgc atgatctggt aaacacgttt
      481 ggcattgatc gcaggcatac catcatgtgc acaaaaaccc accgagcttt tacacggtgg
      541 gtcccagaga aatgcaggac atcaacacgc cctgcatttt tattatcaga aggtatattt
      601 cacacccaaa ataccttggg tgtctctgta actgttatta cccaattgct gagatacgtt
      661 tccccagatg cttaggttat ctgcaagacg cccccccaca ccagttttga gttcaatcac
      721 attacggttg cctatctggt gatttgacat gccattcata attacaccat attgctttga
      781 gctccatttc caattcactt ccaaaaatgg ttcgaactta cgaagagtct ccttatcgat
      841 acaactctga ctaattaagg atgtacgcaa tcccaatttt gcttgaatat tattgacccc
      901 agaggatgtg accatagtgc cattgtgttc aatatagtcg tttgctttta ccccatgcct
      961 agttatctgg acttgtggat tgagatacaa tgcgttgtca atattattat gagcagtcca
     1021 gcgtatggtt gggtagatat agccagcttc caaagctcct gttattcctt tgctggaata
     1081 tttctcacca gttaacccat ctcctttgac tgtattatta aaccaaccgt actgcaacca
     1141 agcatccata tatagacctg ttcgttcttt ttcattctga aaccaagaac tgtacaaacc
     1201 ggcagtataa ccgctcacag ttcctcgtga actataacta gtatacttat tatgagtttt
     1261 gctatcctga tatccataag ctcccataat acccacatgc aagccatcat gatcagtgaa
     1321 gtttgtcttc aaaatatctc caccaatctg ataaaccatc ctgttagctg tggtttttaa
     1381 ttgtctatca gctaaattat gctcattatg tccaccaaca gtacgaatcc acatggtggt
     1441 ttcgctgagc tgacctgtag taggatcaac aatagtgcta ccgtgtcggt catagagaga
     1501 aggactaaac atcgtgttca ttgctatcag gttagcaata taactgccag cttcagggcg
     1561 ccatgtgtga atactacctt cacccaaatt aagtgaagat acaagctgtg aactagctcg
     1621 gcgactactc atttgagtag actcttgatt gctcatctgt tttgattctt gattatccat
     1681 ctgactagtt agataccact tattggtatt cagtccagat acagtgccct gtttcaggcg
     1741 atagtcataa ctaccagcaa caatacggcc tttctgaata aaagcattct tatcagaaga
     1801 atctgtcgaa ataatttgaa caccttcaag agttttatct ccagagcctc cctcatttgt
     1861 cactgcaaca taggtaatac cagacgcatg gcctgcaatt tccagccggt cagttttact
     1921 gttgtcattg ccgagaaccg cgcccaaaga taatattccg ccctttccat gccaattacc
     1981 tttctgaaca taggtctgac cgacatttga cgaactatta ttgagaataa catgaccgtt
     2041 tttctccaga gtcatgttac ctgtgacaat gacggggtca ggcaaaaaag gggcattaat
     2101 attattgatt agcacagtcc cactatttaa taaagtgtta acagtttggt tcatgcctga
     2161 aaaattcaag gtagcacctt tattaacaat aacaccagcg gtacgtgtca tagcttcaac
     2221 tgtccccatt tttagaatgc ctgcattgat gttggtgaaa ccagagtagg tatttttttc
     2281 cgccaaaatg agagttccct cccctgattt tgttagggat tttccgtccc agctatttat
     2341 attatgatgg ctgagattat ctgccagaat agtactaaca gtaagttcag cgttttcttt
     2401 cagattgaac tctccatgac tgtcgccatc tgtatcattc cagctcaaac cgtagatcaa
     2461 tttattctgg tcactgatat agctttttgt atagtcaata gcaccagtag tgagtaacgg
     2521 attgatgata gatatattag aaacactgaa acctgttatc cctttatctg cacttagcag
     2581 tttatagtcc ttctgcgccc agttatttat aatagcttcc acttttacat tgttgccaaa
     2641 tgacatattt ccagaagctt ccagactact gattccagct aaatttaaag aactgttctc
     2701 accaaaagaa acgtcacctg aaacagtgaa tttgttacct gatagtgaaa cagttgtaca
     2761 atcaccagca cataaatcac cttcaatagt tggtgtggtc ccagtgtaat cattgttgat
     2821 aattttcaga atggatgaat tgttaacctg tacatcacca gtaatgacag aaccttcatg
     2881 gagtattaat gaattgtttc caccagtgat gtggatagca ttacctacta cattttgtgt
     2941 tccatcatct tctttacctg aaattatata tccgctatta gcaatggtca tattgctacc
     3001 atttatagca tccccccaat gtccaccatt accgcctaaa atagctccat tgttgattat
     3061 agacaggtta tctcctgtga tagcattacc accgtagcca tcataaccat aaccgtaagc
     3121 agcacctccg ttgcctccag cgaacgtgcc cttattgatt acagagaggc tgcttcctgt
     3181 gatggcatca cctccattac cgccatcacc ctcaccataa ttgttacctc cattaccacc
     3241 ggaaatagtc cctttgttga ttatgggtag attgactcct gtgacggcat cacctccatt
     3301 ccccccatca ctatcactat aactatcgcc accatgtcca cctgaaataa tttctccatt
     3361 tattataaac agattgtctc ctgtaacagc ctcaccacca tcaccgttat ggtcagcacc
     3421 gctaccgccg ttaccaccaa gaatcatgcc ttgattgatt atagacaagt cagatccggt
     3481 aatagagtca ccaccattac cgccacaact attaccatca ttattatcac cagcaccacc
     3541 atgacctgcg ctaatcataa tatcagagtt aatccgtagc ttagttctag atgcatgaga
     3601 ggggttggaa gaagttatat accacgcatc cattccatct tcaccagctc ccaagggaga
     3661 aagtccatca acaggtgtta ataatttttc ataattgtcc tctgaaaaat gaagttcttg
     3721 agtacccgaa agaggaatag caaaagctat tggccccccg agcaacaggg atgcaccaac
     3781 aacaaaagga gaaaaagaca atttgcatgt tttttccttt accgtaggta attctccggc
     3841 ccccccctgt gaacattggg tcatattaca aaaaaatttg tgaatttgat tcatgcacta
     3901 tattatcagt aagtggttga taaaccccta aaaagattcc gttttatccg gaataaaggg
     3961 acgatatatg caaaaatata ttaaacaaag cctcaaccaa aacatgagag ctgtgttata
     4021 tatcaatgca acgggagaaa tttacctgtg tcttgctttg aaaaaaagag ggggaaagct
     4081 atatgttcga taataaatta ataattttac tatgatagtc tgcttcagac cgcaactttt
     4141 cagttctttc tgtacgcttg cccaaagttt aaccactttg tttttctata cacccaatgg
     4201 ttctatctgc cccttgatgt taatccccaa agccaataac atgaatgtat tcatatcaca
     4261 actttcctac cagactctcc aatgcaataa ctgcataatt gtttttagtg gaaatgtatc
     4321 gttttcttaa agagaagaat aacattccat tttattatgt tcccaattta attatattaa
     4381 tatatattgg cattaatagg aaaatacatc aggagaaatc aaatgcagac atcaaacata
     4441 actaaccatg aaagaaatga ctcatcatgg atgtcaactg tcaaatcaac tacagaagta
     4501 agttggaata aattgagttt ttgtgacgtt ttactgaaaa taataacttt cggcatatat
     4561 tctccacacg aaactctagc cgaaaaatat agcgaaaaaa aactaatgga tagtttctca
     4621 ccatcactat cacaagataa aatggacgga gagtttgccc acgcaaacat agatggcatt
     4681 tcaatacggc tatgccttaa caaaggcata tgttcggttt tttatcttga tggagataaa
     4741 atacaatcca ctcaactttc atcaaaagaa tataataact tgttaagttc actccccccc
     4801 aaacaattca acttaggaaa agtacatact attacagctc cggtatcagg gaattttaaa
     4861 actcacaagc ctgcaccaga agttattgaa acagccatta attgctgtac atcgataata
     4921 ccaaacgatg attacttccc cgttaaagac actgatttta atagtgtttg gcatgatatt
     4981 tacagagata ttagagcatc taacagcaat agtacaaaaa tatatttcaa caatattgaa
     5041 ataccactaa aattaattgc agatctaatt aatgagttag gaattaatga attcattgat
     5101 agcaaaaaag agcttcaaat gctaagttac aaccaagtaa ataaaataat caattcaaac
     5161 ttcccccaac aagacctatg ttttcaaaca gaaaaacttt tgtttacttc actctttcaa
     5221 gaccctgcat ttatatctgc actaacatct gcattctggc aatctcttca catcacgtct
     5281 tcctctgtgg aacacatata tgcccaaatt atgtccgaaa acatagaaaa caggcttaat
     5341 ttcatgcctg aacaacgagt tattaacaat tgtggacata ttataaaaat caacgcagtt
     5401 gtccctaaaa acgacactgc aatctctgcc tctgggggga gggcttacga agttagctca
     5461 tcaatattac catcgcacat aacatgtaat ggtgttggta ttaataaaat agaaacaagc
     5521 tatctcgttc atgctggaac acttccaagc agtgaagggc ttcgcaatgc tatccctcca
     5581 gaaagtcgtc aagtatcatt tgccatcata tctcctgatg tttaagccaa tattatattg
     5641 gctgccagac tgtaaaaaaa cgttcttcag aacagctcta taaactatta aaccaaagct
     5701 aaaaggatgg ggaccaatag aatctcagcc catccttttt atatcattat gaaccgcccc
     5761 ggaaatcctg gagactaaac tccctgagaa agaggtaaac aggatgacta aaaatacacg
     5821 tttttcccct gaagtccgtc aacgggcagt tcgtatggtt ctggaaagtc agggcgaata
     5881 tgactcacaa tgggcagcga tttgttccat tgccccaaag attggctgta tactggagac
     5941 tctgcgtgtc tgggtacgcc agcatgagcg ggataccggg ggcggtgagg tgggctcacc
     6001 accgctgaac gtcagcgtct gaaagagccg gaacgtgaaa atcgtgaact gcgccgcagt
     6061 aacaatatcc ttcgcctggc ttccgcttat tttgcaaagg cggagttcga ccgcctctgg
     6121 aaaaaataat gccactgctg gataagctgc gtgagcagta cggggtcgga tcggtatgca
     6181 gtgaactgca tattgccccg tcaacgtatt accactgtca gcaacagcga catcatcatg
     6241 ataaacgcag tgcccgtgcg cagcgcgatg actggctgaa gaaagagata ctgcgcgtat
     6301 acgatgagaa tcatcaggta tacgctgtgc gtaaagtctg gcatcagttg ttacgggaag
     6361 gtatcagagt ggccagatgc actgtggcac gtctcatggc ggttatgggg cttgccggtg
     6421 ttctccgggg taaaaaggtc cacactaccg tcagccggaa agccgttgcc gcaggcgacc
     6481 gcgtaaaccg tcatcaaggc aatatgccgc gtactccggg tggcccgcag cggctggtat
     6541 acgtggtgtc agcggcggac aaggataaac acacgtcagc agttccgtca gcactgcgac
     6601 agcgttgtcc gcaaggcttt tacccagtcc agcgttacgg tgccccacgc ctgacggatg
     6661 aactgtgtgc tctggttacc actttaacgt aaaaaccgtg gcagccagtc tgcgccgtca
     6721 ggaactgagc gcaaaagcct cccagaagtt cagcccgatc agttaccgtg cacacggcct
     6781 gcctgtatca gaaaatctgc tgacgcagga tttttacgcc agtggcccga accagaagtg
     6841 ggcgggagac atcacgtact attacagctc accgactgcg gggaaacacg gggcgccggg
     6901 ttattgaagg tgacctgtcc agctacttcg acacagtaca tcaccggctg ctgatgaaag
     6961 ccgtctgccg caggatcagt gatgcacgtt ttatgagact gctgtggaaa acgccgtgtt
     7021 gatgttggtc tcttccgggc ggtcagtgaa agtatgccac agggcggtgt gatctcgccg
     7081 ttgctgtcga acatcatact gaatgagttc gaccagtatc tgaataaacg ctacctgagt
     7141 ggaaaagcca gaaaggatcg ctggtactgg aatcacagta tccagcgagg ccgaagtact
     7201 gctgtcaaag aaaactggca atggaaacct gccgttgctt actgttgcta tgccgatgac
     7261 tgtgtgccac gaaggcgcgt cctggggacg tgagtccctg tgcagccatg cggcacaaga
     7321 gatgagggaa ggcccctcgg tgtcggcgtg caggcgcagg caccaaacca tccactgctg
     7381 ctgcatttaa gagatgtggt ggtgagcgag atgggaaaag gtcggacgtg agccgatcag
     7441 gtatggttcg aagagacgaa cgaaagtgaa ccgctgatga tgtgtcgaaa gcatatagat
     7501 gatgtcaaaa caatggtgga gtcgttcctg tgggatatgt ttactgcctg cgcggcatcc
     7561 ggcataaaag cggcgtgaag tcgattcagg ctcttgtggg gaacgtggac ctgtcacccc
     7621 gatgtgaagg gagaagcgca agtggaagcc ccatgagcgc cagagtacca atgcggggta
     7681 cagggacgga gcaccaagcg attgcgcagt aaacatgctt gacaacttaa ttggggcccc
     7741 ctcttccttt tatggtgtaa ttgtttgatt gaatacttcc ggggatttca gtatgaaatg
     7801 aagtatattt aatatacttt acaaaaagtt tgtattagtg gaataaggac aatagtttat
     7861 ccaaactgcc aagccccttt attataagta agatctggta ctaaataagc gttctaaacc
     7921 gccccgagta ggcggcctga cggctgcggc ttgtcatggc ctgtgattac cgttataaaa
     7981 acagacaata tcattgtctt tcaggtagtt atatggcccg ttcagctaaa ccacgcaaac
     8041 gaaaacctgc acctcaaaga agcaaactcc tccgctatgt cgtaaaactt cacgaagatg
     8101 atttttttga cgaagaagaa gctgaggttc tgcgctttga taattttgac gatgccgttg
     8161 agtgttgcgc agacctgaat attccgttct ttgtggatgc cggaaacaaa aaactggtct
     8221 tctggtttgt acgtgttgat gacgaagggt atccggaaat tgcccgctgc acggagcggg
     8281 aatttgcgac aattcctgcc ggtatcagcg ccgacggcat gtattgccct gaatgcggta
     8341 cggttcactg gccggatggc gttattccgc ctttctgatg cttccccgtt ttgccgatat
     8401 ttttcagcag ggaaaccgat ggcttaactg gctggagaag cag
//
LOCUS       DAETQN010000163         8401 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.165, whole genome shotgun sequence.
ACCESSION   DAETQN010000163 DAETQN010000000
VERSION     DAETQN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.165"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="K0W68_003874"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="K0W68_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBI3075309.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="K0W68_003875"
     CDS             2089..2238
                     /locus_tag="K0W68_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075310.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3005..3445)
                     /locus_tag="K0W68_003876"
     CDS             complement(3005..3445)
                     /locus_tag="K0W68_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HBI3075311.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3663..4052
                     /locus_tag="K0W68_003877"
     CDS             3663..4052
                     /locus_tag="K0W68_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075312.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4096..4284
                     /locus_tag="K0W68_003878"
                     /pseudo
     CDS             4096..4284
                     /locus_tag="K0W68_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4251..5450)
                     /gene="tet(A)"
                     /locus_tag="K0W68_003879"
     CDS             complement(4251..5450)
                     /gene="tet(A)"
                     /locus_tag="K0W68_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HBI3075313.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            5556..6206
                     /gene="tetR(A)"
                     /locus_tag="K0W68_003880"
     CDS             5556..6206
                     /gene="tetR(A)"
                     /locus_tag="K0W68_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HBI3075314.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(6238..6480)
                     /locus_tag="K0W68_003881"
     CDS             complement(6238..6480)
                     /locus_tag="K0W68_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HBI3075315.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(6786..7622)
                     /locus_tag="K0W68_003882"
     CDS             complement(6786..7622)
                     /locus_tag="K0W68_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBI3075316.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(7622..>8401)
                     /locus_tag="K0W68_003883"
                     /note="aph(3'')-Ib"
     CDS             complement(7622..>8401)
                     /locus_tag="K0W68_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HBI3075317.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1889 a         2322 c         2332 g         1858 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttatacatca cttttacatc attccttcag
     2641 ctcatacgtc actttcatcc tctgtgccat ttgtacatca cccccttcct gaacgtgctg
     2701 acagcgggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc
     2761 gttcaccgtt ttcggatggg aagaacgccg gaaacaggac ttaccccggc tgaatggtgt
     2821 gaatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgcg
     2881 cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg
     2941 ccctgagggc ctctcaggcg gtgctgataa cagatgttat ggtaaatcag agtcacctgc
     3001 actgttactc tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt ttttcctgtg
     3061 ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg ttccctctcc tcatttcgca
     3121 ggcggttttc tgcttcccgc gccatcttgt cttcaagcat tagggtaaca gtctgctgca
     3181 gagcgtccag tttctgctga atgacgtcta gctgagcatt ttccgcctgg ctaacgacag
     3241 atttggagtg tccctccttg tgcctttttg gtgtctcaga agtgcgcagc tcgccatagg
     3301 cccgaatcag ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg cgatagctga
     3361 caaggccttt agccatgtca cgatataatt gcgagcgcga ctttcctgtt aatttcagtg
     3421 tctcttgaac tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt accgtattgt
     3481 aacagcagaa catattactg ttttaacaca aaaggtctga ggctgcccgt gaggcgtgaa
     3541 aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt cgaatactgt
     3601 atataaacac attgttataa atacagtttt tgctgttttt aaaagtcaaa gaggataatt
     3661 ttatgagtac agagttaaat agcggtactg tcggtggttt tggtcacatg ggaccaaatg
     3721 acccctacaa tgatggtcat agcggctctg gaagttcggg aggtagtttt aacggtggtg
     3781 gtaatggtgg ccatgataat agtagtggtt catcttatac tgcatccttt ggtctcatca
     3841 ctggccgaca cccgataact gtcccaggta cgaagaatat taattctgtc gatctttctg
     3901 ctgtcattaa taacattata catcatgaag gtgcacgcaa taacatgtac ctagatactg
     3961 cgaacaatgt aagagtggga gtgacgggca ctggctggca atgtctagca acggcaggca
     4021 tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt
     4081 gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact
     4141 ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat
     4201 tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa
     4261 cgggaatttg aagaatttct ccaaagcccg cgacgcagcg ccggcaggca gagcaagtag
     4321 agggcagcgc ctgcaatcca tgcccacccg ttccacgttg ttatagaagc cgcatagatc
     4381 gccgtgaaga ggaggggtcc gacgatcgag gtcaggctgg tgagcgccgc cagtgagcct
     4441 tgcagctgcc cctgacgttc ctcatccacc tgcctggaca acattgcttg cagcgccggc
     4501 attccgatgc cacccgaagc aagcaggacc atgatcggga acgccatcca tccccgtgtc
     4561 gcgaaggcaa gcaggatgta gcctgtgccg tcggcaatca ttccgagcat gagtgcccgc
     4621 ctttcgccga gccgggcggc tacagggccg gtgatcattg cctgggcgag tgaatgcaga
     4681 atgccaaatg cggcaagcga aatgccgatc gtggtcgcgt cccagtgaaa gcgatcctcg
     4741 ccgaaaatga cccaaagcgc ggccggcacc tgtccgacaa gttgcatgat gaagaagacc
     4801 gccatcaggg cggcgacgac ggtcatgccc cgggcccacc ggaacgaagc gagcgggttg
     4861 agagcctccc ggcgtaacgg ccggcgttcg cctttgtgcg actccggcaa aaggaaacag
     4921 cccgtcagga aattgaggcc gttcaaggct gccgcggcga agaacggagc gtggggggag
     4981 aaaccgccca tcagcccacc gagcacaggt cccgcgacca tcccgaaccc gaaacaggcg
     5041 ctcatgaagc cgaagtgccg cgcgcgctca tcgccatcag tgatatcggc aatataagcg
     5101 ccggctaccg ccccagtcgc cccggtgatg ccggccacga tccgcccgat atagagaacc
     5161 caaaggaaag gcgccgtcgc catgatggcg tagtcgacag cagcgccggc cagcgagacg
     5221 agcaagaccg gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg tgcgcaggca
     5281 aattgcatca acgcatacag cgccagcaga atgccatagt gggcggtgac gtcgttcgag
     5341 tgaaccagat cgcgcaggag gcccggcagc accggcataa tcaggccgat gccgacagcg
     5401 tcgagcgcga cagtgctcag aattacgatc aggggtctgt tgggtttcac gtctggcctc
     5461 cggaccagcc tccgctggtc cgattgaacg cgcggattct ttatcactga taagttggtg
     5521 gacatattat gtttatcagt gataaagtgt caagcatgac aaagttgcag ccgaatacag
     5581 tgatccgtgc cgccctggac ctgttgaacg aggtcggcgt agacggtctg acgacacgca
     5641 aactggcgga acggttgggg gttcagcagc cggcgcttta ctggcacttc aggaacaagc
     5701 gggcgctgct cgacgcactg gccgaagcca tgctggcgga gaatcatacg cattcggtgc
     5761 cgagagccga cgacgactgg cgctcatttc tgatcgggaa tgcccgcagc ttcaggcagg
     5821 cgctgctcgc ctaccgcgat ggcgcgcgca tccatgccgg cacgcgaccg ggcgcaccgc
     5881 agatggaaac ggccgacgcg cagcttcgct tcctctgcga ggcgggtttt tcggccgggg
     5941 acgccgtcaa tgcgctgatg acaatcagct acttcactgt tggggccgtg cttgaggagc
     6001 aggccggcga cagcgatgcc ggcgagcgcg gcggcaccgt tgaacaggct ccgctctcgc
     6061 cgctgttgcg ggccgcgata gacgccttcg acgaagccgg tccggacgca gcgttcgagc
     6121 agggactcgc ggtgattgtc gatggattgg cgaaaaggag gctcgttgtc aggaacgttg
     6181 aaggaccgag aaagggtgac gattgatcag gaccgctgcc ggagcgcaac ccactcacta
     6241 cagcagagcc atgtagacaa catcccctcc ccctttccac cgcgtcagac gcccgtagca
     6301 gcccgctacg ggctttttca tgccctgccc tagcgtccaa gcctcacggc cgcgctcggc
     6361 ctctctggcg gccttctggc gctcctgctg cggcgtccgc tcgtgggccg tggcgcgggt
     6421 ccgcgcgccg gcctcgtgcg cctggcgctc gcgggcgagg tccagggcgg ccgtcttcac
     6481 gttctgcctt gcgcagatga gatagatcta gcgtggactc aaggctctcg cgaatggctc
     6541 gcgttggaaa ctttcattga cacttgaggg gcaccgcagg gaaattctcg tccttgcgag
     6601 aaccggctat gtcgtgctgc gcatcgagcc tgcgcccttg gcttgtctcg cccctctccg
     6661 cgtcgctacg gggcttccag gggtcgtttg cgggaagggg cggaatccta cgctaaggct
     6721 ttggccagcg atattctccg gtgagattga tgtgttccca ggggatagga gaagtcgctt
     6781 gatatctagt atgacgtctg tcgcacctgc ttgatcgcgg ccgcgatagc tagatcgcgt
     6841 tgctcctctt ctccatccgc gttccaagct gcggaaaggc acccataagc gtacgcctgg
     6901 tcgagcaggc gacgcggatc gacgtccagc gcacgagaga atgcgtccgc catctgtgca
     6961 atgcgtctag gatcgagaca aaggtcgtct ctgtcagccg gatcgtagaa catattggcg
     7021 gcgccaaagc ccacttcacc gaccagaccg acgggatcta tcaccagcca gccgcgactg
     7081 gagaacatga tgttttcatg atgcagatcg ccatgtagcc cacgcagttc cgaggcattg
     7141 ctcatcattt gatcggctat aatcgccgcg tggacgtagt cagtttgaca acctgcgttt
     7201 tgatcatcgc gcgcccgctg aaacaaagct gcaaagcgat cccggatcgg gagaagggca
     7261 gaaggcaggg gttcctcaga tgcggcatac agcttcgcca ttagttccgc tgcaatttcg
     7321 gtcgcctggt agtcgccgtg ctcggcaacg atgtgagaga gcattcgctc cccggcatat
     7381 tcgagcaaca tcagattgtt ctcacgaccg agcaaccgga ctgctcccct cccattgcgc
     7441 cataccagat agtcggcccc gcgcagttca tcagcaatgt cttctatagg tttcaatccc
     7501 ttgacgattg caggagtccc gtctggcaat gaaactttcc aaacgaggct ggaaaaggtg
     7561 tccgcaatga gaacaggttg cgaaacgtgc caatgagcag gaaaaacagg cggcatgaac
     7621 atcaacccca agtcagaggg tccaatcgca gatagaaggc aaggcgttcg cggtcggggg
     7681 cttcgatccc caatacattg aataggacag cgaaggcgcg ctctgcttca tctggcgctg
     7741 cccagttctc ttcggcgtta gcaatcatga gtgccaaatc ggcatagcga tctgctgttc
     7801 cgagccgccc aaggtcgatc agacccgtgc attgaagagt tttagggtcc accatgaagt
     7861 tcggcatgca gggatcacca tggcaaacaa ccatatcggt gcgctcttgg tcgagccgca
     7921 ccggtagctc tcgttcgaca cgagccaaaa gatcgagctg cggcgtactc ttgtcctcgt
     7981 ccggtaagaa gtcgggattg acggcattgc gggacaccac atcaacggcg cgtccgaaca
     8041 ttcgcgacag cctgcgctca aactgacatt gatcaaccga taggctgtga acagcgccaa
     8101 gttgctgccc cattgacggc cacgctttga gcaaatccgc tccagacaga tcagccgccg
     8161 gtactcccgg aattgccgtt atcaccaagc atgcaccctc ctgttcctcc tgccagttga
     8221 tgacctcggg gcaagccaca cctcgacctt tgagccaaat gaggcggtca cgctctccag
     8281 cgagctcacc gcggcgggaa gcaggtgcga ttttcgcgaa ggcatgcccg tcaccacgtc
     8341 gaaaaacaaa atcaccagat tctccgcctc tgacaggcaa ccagtcagaa tgcgattcac
     8401 c
//
LOCUS       DAETQN010000164         8310 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.166, whole genome shotgun sequence.
ACCESSION   DAETQN010000164 DAETQN010000000
VERSION     DAETQN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.166"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..617
                     /locus_tag="K0W68_003884"
                     /pseudo
     CDS             1..617
                     /locus_tag="K0W68_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1231..1607)
                     /gene="rnpB"
                     /locus_tag="K0W68_003885"
     ncRNA           complement(1231..1607)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="K0W68_003885"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1640..2785)
                     /gene="garK"
                     /locus_tag="K0W68_003886"
     CDS             complement(1640..2785)
                     /gene="garK"
                     /locus_tag="K0W68_003886"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HBI3075318.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2882..3772)
                     /gene="garR"
                     /locus_tag="K0W68_003887"
     CDS             complement(2882..3772)
                     /gene="garR"
                     /locus_tag="K0W68_003887"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBI3075319.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3802..4572)
                     /gene="garL"
                     /locus_tag="K0W68_003888"
     CDS             complement(3802..4572)
                     /gene="garL"
                     /locus_tag="K0W68_003888"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HBI3075320.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4588..5922)
                     /gene="garP"
                     /locus_tag="K0W68_003889"
     CDS             complement(4588..5922)
                     /gene="garP"
                     /locus_tag="K0W68_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBI3075321.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            6297..7868
                     /gene="garD"
                     /locus_tag="K0W68_003890"
     CDS             6297..7868
                     /gene="garD"
                     /locus_tag="K0W68_003890"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBI3075322.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            8017..8121
                     /gene="prlF"
                     /locus_tag="K0W68_003891"
                     /pseudo
     CDS             8017..8121
                     /gene="prlF"
                     /locus_tag="K0W68_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
     gene            8186..>8310
                     /locus_tag="K0W68_003892"
     CDS             8186..>8310
                     /locus_tag="K0W68_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3075323.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLG"
BASE COUNT         2183 a         2174 c         1956 g         1997 t
ORIGIN      
        1 caacatgttc caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta
       61 tttactaaaa ataacgcctg ttttaataag cattagcgat atgtattctg aagaaaaaat
      121 caagtttgtc gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat
      181 tatgagattt gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa
      241 tgaattaaca aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat
      301 taaattacca gagatgaaag aattctcatc agaaaaaaat atatgattac atcgatataa
      361 tcattgaaga ttatgaaaat atcaaagaaa atgcttatct ggtcgatcca aaaattaatt
      421 acactataga tttaaacata gaagactcta gctcagagga attccttttt gataatagcc
      481 tcgagaaaga cgaaaattct tcggacaatt gctttgaggt cgaaaaatat aacgcatatg
      541 aagcatataa ctcagagaat ctatatttta ccagagagga atatacgtat gattacgacc
      601 ttttaaatgc aatatagaca taataacttc ttacaaaatc tataattaat accgtagata
      661 ttcattcatc ttaccctgtg caccaaaagc aaaaactcac tgacgcgggt ttgaaactga
      721 tggcaaccgc aaaaatgcct gatgcgctac gcttatcagg cctacgccat ctctacaata
      781 tattgaattt gcgtgctttt gtaggcagga taaggcgttc acgcagcatc cggcatgaat
      841 aaagcgcact caacaatctg aaacccgccg gaacggttta ttacgtacat caggtgaaac
      901 tgaccgataa gccgctttct tttgggtata gtgtcgtgga cagtcattca tctttctgcc
      961 cctccaaaag caaaaacccg ccgaagcggg tttttacgta aatcaggtga aactgaccga
     1021 taagccgctt tcttttgggt atagcgtcgt ggacagtcat tcatctttct gcccctccaa
     1081 aagcaaaaac ccgccgaagc ggttttttac gtaaaccagg tgaaactgac cgataagccg
     1141 ctttcttttg ggtatagcgt cgtggacagt cattcatctt tccgctcctc caaaagcaaa
     1201 aacccgccga agcgggtttt tacgtaaatc aggtgaaact gaccgataag ccgggttctg
     1261 tcgtggacag tcattcatct aggccagcaa tcgctcactg gctcaagcag cctacccggg
     1321 ttcagtacgg gccgtacctt atgaacccct atttggcctt gctccgggtg gagtttaccg
     1381 tgccacggac tgttaccagc cgcgcggtgc gctcttaccg caccctttca cccttacctg
     1441 atcccgcttg cgcgggccat cggcggtttg ctctctgttg cactggtcgt gggtttcccc
     1501 cccaggcgtt acctggcacc ctgccctatg gagcccggac tttcctcccc tccgcccgtc
     1561 tcccccgaag aggacgacga cgaagcggcg actgtctggt cagcttcggc gcgcagtata
     1621 gagggtttgc gcgcccttgt caccccgcgt tgcgcattcc aatcgccagt gtcgcggcga
     1681 tattacgtga agcacggcag atattgtcat aagccccgcg gaatgcttcg tccaacgtac
     1741 ctatgctggt caatacgctg aagaccgcat cgatgccatg ctgatgtaca acgccaacat
     1801 catcggtcag gctacccgca atgccaatca ccggtttatg gtacttcttc gccacgtttg
     1861 caacgccaat cggtaccttg ccgtgaatac tctggctgtc aatacgccct tcaccggtga
     1921 tcaccagcgt acaatcgtga atatgttctt ccagattcag cgccgtagtg acgatttcaa
     1981 taccactttt cagttccgca ccaagaaacg ccatcagcgc cgcgcccata ccgcccgcag
     2041 ctcctgcgcc ggggacatct ttcacatcaa catgcagcgc ttttttaatg acatcggcat
     2101 agtgagagag gttattgtcc agctcaacaa tcatcgcttc actggctccc ttttgtgggc
     2161 caaagatgcg cgatgcgccg ttatcgccca ccagcggatt ggtgacatca caagcgacgc
     2221 gaatgacgca atcttttaag cgcggatcga ggccggaaat atcaatatca ttcagagtat
     2281 taagactacc gccgccaaaa ccaatttcat tgccgttggc gtcgcataat ttcgccccca
     2341 gcgcctgtac catgcctgcg ccgccatcat ttgtagcgct gccgccaatg ccgataataa
     2401 tgtttgttgc actgctctcc agcgcctgca ggattaactc gcctgtgccg cgtgaagtgg
     2461 ttacgagtgg atcgcgtttt tccgcaggta ccagctccag cccactggcc gccgccattt
     2521 caataaacgc ggttttgcca tcgccggaga tcccccaact ggcattcact ttctcgccca
     2581 gcggccctgt aacccaggcg tgacgttcag ccccctgggt ggctgcaatc atcgcttcca
     2641 ccgttccttc accaccgtcg gcaaccggaa cagaaacata ctgtgcatca ggaaaaattt
     2701 cccgaaatcc tttttctatc gcctgcgcaa cctcgctggc agataaactt tctttataag
     2761 agtctggggc gattacgatt ttcataccta tgcctgttac cacatgacgc cggagggcgt
     2821 ttctcttatt cggcctagat tccaggcccg gattgcaata caccatccgg gcacgacgtc
     2881 attaacgagt aacttcgact ttcgccagtt tttcgtagta gcacgccagg gcgctatgat
     2941 ccgccgttcc taaaccatct gcacgcagtg cctgcatcat ctccataacc gcagctgtga
     3001 gcagcagttg tgcgccgacg ccgtgagaag tatccagcgc attcgccaga tccttaatat
     3061 gcagatcaat gcggaagccc ggcttgaagt tgcggtccat caccatcggc gctttggcat
     3121 ccagcacggt actgcccgcc agcccaccgc gaattgcctg ataaaccagg tccgggttaa
     3181 cgcccgcttt ggttgccagc gttaacgctt ctgacatcgc agcaatattc agcgccacaa
     3241 tgacctgatt tgccagtttg gtgacgttac ctgcaccgat ttccccggta tgcaccacgg
     3301 aacccgccat cgctttcatc aaatcatagt atttgtcgaa aatagccttg tcgccgccca
     3361 ccatcactga cagcgtaccg tcgatggctt tcggttcacc gccgctcacc ggagcatcca
     3421 gcatatcaat gcctttcgct ttcagcgctt cgctgatttc acggcttgcc agcggtgcga
     3481 tagaactcat atcgatcaat accgtacctg gcttcgcgcc ttcaataatg ccattctcac
     3541 ccagcgccac ctctttcaca tgaggggagt ttggcagcat ggttatgatg acgtcgcact
     3601 gttcagcgat cgctttagcc gtagacgctg tttctgcacc tgcagcaatc acgtcagcaa
     3661 tagcttctgg gttacggtca gcaaccacca gcgagtaacc tgctttcaga aggtttttac
     3721 tcattggttt acccataatc cccaggccaa taaaaccaac tttcatagtc atatcaacca
     3781 tctctcttgt tgcggtggtg gttatttttt aaaggtatca gccagtttct gagtggcaga
     3841 gcggaagacg ccaagatcgc tgccgacagc cacaaacgtc gcgccccatt ccagataacg
     3901 acgcgcatcg gcttcgaccg gcgcgagaat accgctgggt ttgccgtgcg cgctggcacg
     3961 gttaaaaata tgctgaattg ctttttgtac atccggatgc gaggcattgc cgagatggcc
     4021 caatgccgca gccagatcgc tggggccgac gaagataccg tctacacctt cggtagcggc
     4081 aatagcatcg acgttatcta cgccctgctg actttctatc tggaccagaa tagtaatgtt
     4141 cttgttcgac tgagcgaaat aatccgccac ggtgccaaac atattggtgc ggtgagaaac
     4201 ggagacgccg cgaatgcctt ccggcgggta acgagttgat gccaccgcct gctctgcttc
     4261 ctcttttgtt tctacaaaag gaatcaggaa gttatagaaa ccgatatcca gaagacgctt
     4321 aataattacc ggctcgttgg tcggcactcg cactactggc gcgctggcgc tgcctttcag
     4381 ggccattaac tgcggaataa acgtggaaat atcgtttggc gcatgttcgc catccagcac
     4441 cagccagtca aacccagcca aaccaagaac ttcagtgcta atcgggttag agagtgctga
     4501 ccagcaacca atttgtacct gtttcgcagc cagtgcggct ttgaatttat tcgggaaaac
     4561 atcgttattc atcgcttata cctttgctta tttctgcaat tccatacgtt taatgtcgcc
     4621 aactacgaag aggtagcaga ccatcgccat cagcgctgaa catcccacga aaaccagtgc
     4681 tgcattgaag gagtgcagtt cacttaccag gtagccaatc accagtggag tgacaatgga
     4741 ggcaacattg ccaaagacgt taaagacgcc gccacagagg ccaacaatct ctttcggcgc
     4801 ggtgtcagaa atcaccggcc agcccagagc accaaatcct ttgccaaaaa aagccagcgc
     4861 catcagcatg accaccagcg tggtgttgtt ggtgtagtta cataagatga tggtggaaac
     4921 cagcaacatt cccagcacaa tcggtagctt acgtgccagg gtcagggata aaccgcgttt
     4981 gatcagataa tccgagaaga cacctcccag cacgccgccc gcaaaaccac acagtgccgg
     5041 aatcgaggcg accagaccca ctttcagaat cgacatgcct ttttcctgca ccagataaat
     5101 cgggaaccag gtgaggaaga accaggtgat ggtgttgata aaatattgtc cgaaaaatac
     5161 gcccaacatc atgcggttag agagcaattg cttgatgtaa tgcagtttgg gaccgcttgc
     5221 tgccgcgctg cccggctttt tgtggtccat atcgaccacc gcgccatttt cagagataaa
     5281 cttcagctct tccgcagaca tacgtgggtg atctgtcggg ttatgaatca acttgatcca
     5341 cagcgccgtc agcacaaaac caatcccccc cataacggtg aagacgtgct cccagcccca
     5401 agcgaaagtc agccagccaa gcagcggcga aaagagcgcc agcgagaaat attgcgccga
     5461 gttaaagatg gcggaggcag taccacgttc tttcgtcggg aaccaggcgg cgacaatacg
     5521 ggcgttcgcc gggaatgatg gcgcttccga gaaccccagc ataaagcgca taaagaacat
     5581 ggagatccct gcccaggcca gcgggaacat atcaacaaag ccttgcagga aggtgaacag
     5641 cgaccagaaa aagaggctgt aggtgtaaac ttttttcgag ccaaacttat caagcaacca
     5701 gccgccgggg atttgcatca gcaagtaggc ccagccaaaa gcagagaaga tgtaacccat
     5761 cgaaaccgca cttaactgca actcttttgc cacttcggta ccagcaatag acagcgttgc
     5821 acgatcggcg tagttaacgg cggtaacaat aaaaataatc agtaatatta aatagctggt
     5881 atgcacgcct ttctttttta cgtcaacggt gtccagaatc attttattta cctcgggtac
     5941 ttatgctgat ttttattatt atggggaagg tgttatttat gagttttatt tatgccgtta
     6001 cgacaatgaa ctcgggaatt agtataagca gcgggagaat aataatcatt gtgcaaatgc
     6061 taatttaatc agtactattt aaatattatt ttgagcatat gcacataagg ttgcgcgcta
     6121 aagcacagat ttgcgcttta ccttaccggg cggcactgca atccctgaaa tgattgacat
     6181 tgatcacatt tctgcgttta aactcctgac attcttattt cacccaatga agtcatttat
     6241 ttttaaatga gaccagatcc tcattttaat aacccctggc tggagaatat tgcacaatgg
     6301 ccaacatcga aatcagacaa gaaacgccaa ctgcgtttta tataaaagtt cacgacacag
     6361 ataatgtggc aattattgtt aatgataatg gcctgaaagc aggaacgcgt tttccggatg
     6421 ggctggaatt aattgaacat actccccagg ggcataaagt cgcattgctg gacattccag
     6481 ctaatggtga aattattcgt tatggcgaag tgattggtta cgccgtgcgc gcaatcccac
     6541 gcggaagctg gatcgacgaa tcaatggttg tactaccgga agcgccgccg ttacacacgc
     6601 tgccactggc aaccaaagtc ccggccccct taccgccgct ggaaggatac acctttgagg
     6661 gctatcgcaa tgccgatggc agcgtgggca ccaaaaacct gctcggcatc accaccagcg
     6721 tccactgtgt ggcaggcgtg gtggactatg tagtaaaaat cattgaacgc gatctgctac
     6781 cgaaataccc gaacgtcgat ggcgtggtgg ggctgaatca tttgtacggc tgtggcgtgg
     6841 cgattaacgc accggcggca gttgtgccta ttcgtaccat ccacaatatt tcgctgaacc
     6901 ctaactttgg cggcgaagta atggtgattg gtctgggttg tgaaaagttg cagcctgagc
     6961 gcctgctggt cggcacggat gatgtgcaag ctattccagt agaaagcgcc agcattgtca
     7021 gtttgcagga tgaaaagcat gtcggctttc agtccatggt cgaggatatt ttgcaggtcg
     7081 ccgaacgcca tctacaaaaa ctgaatcaac ggcagcgaga aacctgcccg gcttcagaac
     7141 tggtcgttgg tgtgcagtgc ggtggcagcg atgcgttttc tggtgtaacg gcaaacccgg
     7201 cggttggcta tgcgtctgat ctactggtgc gctgcggcgc aacggtgatg ttttcagaag
     7261 taacggaagt gcgtgacgcg atccatctgc tgacaccacg cgcagtgaac gaagaggtcg
     7321 gcaaacggct gctggaggag atggagtggt acgataacta tctcaatatg ggaaaaaccg
     7381 accgcagcgc caacccttcg ccgggcaaca agaaaggcgg tctggcaaat gtggtggaga
     7441 aggcactcgg ctccattgct aaatcgggta aaagtgcaat tgttgaagtg ctgtcgcccg
     7501 gtcaacgccc gactaaacgc ggattaattt acgccgcgac gccagccagc gattttgtct
     7561 gtggcacgca acaggtggct tcgggtatca ccgtacaagt gtttacgacc ggccgtggta
     7621 cgccgtacgg cctgatggcg gtacccgtaa ttaaaatggc aacccgcacc gagctggcga
     7681 accgctggtt tgatttaatg gatattaacg cgggcactat cgctaccggc gaagagagca
     7741 ttgaagaggt gggctggaag ttgttccact ttattctcga cgtcgccagc gggaagaaga
     7801 aaaccttctc ggatcaatgg gggctgcata accagctggc ggtgtttaac ccggcaccgg
     7861 tgacctgatt tcttttggaa cgtccctcgc aaaacatggc cttagtgcca tgtttttatt
     7921 gtttaaagcc cccacgtcca ttaataatgc atttgcatta ccttagttca agcttataat
     7981 ttgagcagaa aacaggctgt aaaaggacaa tgaatcatgc ccgctaatgc tcgctctcac
     8041 gctgtactga ccactgaatc aaaggtcacg atacgcggac aaacaactat ccccgcgcca
     8101 gtgcgtgagg ccttaaaact gtgaggtagc ctgagtttaa cggacactcc ttcctgaaat
     8161 agaatggcat cagaaggagc taataatgag cagaaaaacc caacgttact ctaaagagtt
     8221 caaagccgaa gctgtcagaa cggttcttga aaatcaactt tcgatcagtg aaggcgcttc
     8281 ccgattatct cttcctgaag gcactttagg
//
LOCUS       DAETQN010000165         8273 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.167, whole genome shotgun sequence.
ACCESSION   DAETQN010000165 DAETQN010000000
VERSION     DAETQN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.167"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            52..936
                     /locus_tag="K0W68_003893"
     CDS             52..936
                     /locus_tag="K0W68_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBI3075324.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="K0W68_003894"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="K0W68_003894"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HBI3075325.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="K0W68_003895"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="K0W68_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBI3075326.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="K0W68_003896"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="K0W68_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBI3075327.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="K0W68_003897"
     CDS             5160..6443
                     /locus_tag="K0W68_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HBI3075328.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8065
                     /locus_tag="K0W68_003898"
     CDS             6590..8065
                     /locus_tag="K0W68_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBI3075329.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         2192 a         1980 c         1915 g         2186 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaagtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacataatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta cccacgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 ctgtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgccc gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccgat acccggggtc actgtcaggc
     2221 cgccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agtcagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccaat aagcggtaat tgaaagtttg tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgt tcaacgacac
     2581 cgacgcctgc gcccagggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg acgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gccttgtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg agcctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcatttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gctatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacaga acccggtgat gagaacaacc aggacatctc
     3781 cgcgctggta gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aatttgtcga
     3901 gatgtttaaa gcaccgatta aagtgctgca tccgctgtta accgctactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgaatcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt acgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aaaggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tagaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct ataacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgaccgggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc attaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccgtatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caggcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccag gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tggtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacgtta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca tttttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgc tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
     7261 tgtcgtcgac tactgtccgg acaaccatac tgcgacctgg ggaatcaact atacaaaaat
     7321 attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag
     7381 cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct
     7441 actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga ttgaagtttc
     7501 cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcagaaac tgcaactctc
     7561 cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa
     7621 cagcgtagag tcattattgt cgagacctgt tgttaaggac ttctgtgtca tgctggttga
     7681 tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgggtg ataaagtgct
     7741 ggtttcaatt gtcgatatta tccagcaaag catccgcccc gatgatattt tagcgcgact
     7801 ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat
     7861 tgcggaacgt atgcgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt
     7921 tcctgaacaa atgaccatca gtattggcac ggttttttca acgggtgaca cgcgtaatat
     7981 ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa
     8041 caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg
     8101 cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat
     8161 gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc
     8221 actgaaccga aggagtgcca tttatcatga aattgcacca tagaatgctc cgg
//
LOCUS       DAETQN010000166         8217 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.168, whole genome shotgun sequence.
ACCESSION   DAETQN010000166 DAETQN010000000
VERSION     DAETQN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.168"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="K0W68_003899"
     CDS             complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="K0W68_003899"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HBI3075330.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="K0W68_003900"
     CDS             complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="K0W68_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HBI3075331.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2031..2549
                     /locus_tag="K0W68_003901"
     CDS             2031..2549
                     /locus_tag="K0W68_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HBI3075332.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2594..4639)
                     /gene="dcp"
                     /locus_tag="K0W68_003902"
     CDS             complement(2594..4639)
                     /gene="dcp"
                     /locus_tag="K0W68_003902"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBI3075333.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4776..5522
                     /gene="ydfG"
                     /locus_tag="K0W68_003903"
     CDS             4776..5522
                     /gene="ydfG"
                     /locus_tag="K0W68_003903"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBI3075334.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5611..6297
                     /gene="ydfH"
                     /locus_tag="K0W68_003904"
     CDS             5611..6297
                     /gene="ydfH"
                     /locus_tag="K0W68_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HBI3075335.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6474..6677
                     /gene="ydfZ"
                     /locus_tag="K0W68_003905"
     CDS             6474..6677
                     /gene="ydfZ"
                     /locus_tag="K0W68_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBI3075336.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6713..8173)
                     /locus_tag="K0W68_003906"
     CDS             complement(6713..8173)
                     /locus_tag="K0W68_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBI3075337.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2173 a         2055 c         1871 g         2118 t
ORIGIN      
        1 gaacggagat aatccctcac ctaaccggcc ccttgttaca gttgtgtaca aggggcctga
       61 tttttatgac ggcgaaaaaa aaccgccagt aaaccggcgg tgaatgcttg catggataga
      121 tttgtgtttt gcttttacgc taacaggcat tttcctgcac tgataacgga tcgttgacac
      181 agtagcatca gttttctcaa tgaatgttaa acggagctta aactcggtta atcacatttt
      241 gttcgtcaat aaacatgcag cgatttcttc cggtttgctt accctcatac attgcccggt
      301 ctgctcttcc aatgaccaca tccagaggct cttcaggaaa tgcgcgactc acacctgctg
      361 tcacggtaat gttgatatgc ccttcagaat gtgtgatggc atggttatcg actaactggc
      421 aaattctgac acctgcacga catgcttctt catcattagc cgctttaaca ataatgataa
      481 attcttcgcc cccgtagcga taaaccgttt cgtaatcacg cgtccaactg gctaagtaag
      541 ttgccagggt gcgtaatact acatcgccga ttaaatgccc gtaggtatca ttaaccaatt
      601 taaatcggtc aatatccaac aacattaaat aaagattcag aggatcagtg ttgcgtaact
      661 gatgatcaaa ggattcatca agaacccgac gccccggcaa tcccgtcaaa acatccatat
      721 tgctacggat cgtcagcaaa taaattttgt aatcggttaa tgccgcagta aaagaaagca
      781 acccctcctg aaaggcgtcg aaatgcgcgt cctgccagtg attttcaaca atagccagca
      841 ttaattcccg accacagtta tgcatatgtt gatgggcaga atccattagc cgaacgtaag
      901 gtaattcatc gttatcgagt ggccccagat gatcaatcca ccgaccaaac tggcacagtc
      961 cataagaatg gttatccgtt atttctggct tactggcatc tctcgcgacc acgctgtgaa
     1021 acatactcac cagccactgg tagtgggcat cgatagcctt attgagattt aacaagatgg
     1081 catcaatttc cgttgtcttc ttgatcattg ccactccttt ttcacagttc cttgtgcgcg
     1141 ctattctaac gagagaaaag caaaattacg tcaatatttt catagaaatc cgaagttatg
     1201 agtcatctct gagataacat tgtgatttaa aacaaaatca gcggataaaa aagtgtttaa
     1261 ttctgtaaat cacctctgca ttatcgtaaa taaaaggatg acaaatagca taacccaata
     1321 ccctaatggc ccagtagttc aggccatcag gctaatttat ttttatttct gcaaatgagt
     1381 gacccgaacg acggccggcg cgcttttctt atccagactg ccgctaatgt tgatcatctg
     1441 gtccggctga acttctcgtc catcaaagac ggccgcagga ataacaacat taatttcacc
     1501 gctcttatcg cgaaaaacgt aacggtcctc tcctttgtga gaaatcaaat taccgcgtag
     1561 tgaaaccgaa gcgccatcgt gcatgttttt tgcgaaatca acggtcattt tttttgcatc
     1621 atcggttccg cgatagccat cttctattgc atgaggcggc ggtggcgctg catcctgttt
     1681 taaaccgccc tggtcatctg ccaacgcata aggcatgaca agaaaacttg ctaatacaat
     1741 ggactgaaat ttcatactaa ctccttaatt gcgtttggtt tgacttatta agtctggttg
     1801 ctatttttat aattgccaaa taagaatact gccaattgtt ataaggcatt taaaatcagc
     1861 ccactagctg tcaaatattc agagaatttg actcactaaa gttaagaaga ttgaagagtc
     1921 ttaaacatgt tttcagaata atcggattta tatgtttgaa aattattata ttggacgagc
     1981 atacagaaaa agcaaatcac ctttacatat aatagcgtgg acaaaaaaca gtgaacatta
     2041 ataaagataa aattgtacaa ctcgcagata ccgacactat tgaaaacctg acatccgcgt
     2101 tgagtcaaag acttatcgcg gatcaattac gcttaactac cgccgaatca tgcaccggcg
     2161 gtaagttggc tagcgccctg tgtgcagctg aagatacacc caaattttac ggtgcaggct
     2221 ttgttacttt caccgatcag gcaaagatga aaatcctcag cgtaagccag cagtctcttg
     2281 aacgatattc tgcggtgagt gagaaagtgg cagcagaaat ggcaacaggt gccatagagc
     2341 gtgcggatgc tgatgtcagt attgccatta ccggctacgg cggaccggag ggcggtgaag
     2401 atggtacgcc agcgggtacc gtctggttcg cgtggcatat taaaggccag aactacactg
     2461 cggttatgca ttttgctggc gactgcgaaa cggtattagc tttagcggtg aggtttgccc
     2521 tcgcccagct gctgcaatta ctgctataac caggctggcc tggcgatatc cccaggccag
     2581 ccattggtgg tgtttatatg ttcaagccac gatgttgcag catcggcata atctgaggag
     2641 ccttaccgcg ccattgtcga tgcaggcgtt ccagatcttc gctgttacct ctggaaagga
     2701 tcgcctcgcg aaaacgctgc ccattttcac gcgttaatcc gccctgctca acaaaccact
     2761 ggtaaccatc atcggccaac atttgcgtcc acagataagc gtaataacct gcagcatatc
     2821 cgccaccaaa aatatgggcg aaataactgc tgcgatagcg tggcggtata gcaggaagat
     2881 ccatattttc cgccaccagc gcccgcaatt caaaatcatc gacatcctgc attgcttcgt
     2941 tttcttccag gcaatgccag cgcatatcga gaagtgcggc gctaagcagt tcgctcatct
     3001 catacccttt gttgaacagg ctggcattac gcattttctg ttgcagttcg tcaggcattg
     3061 ctgccccgct ctgataatgc cgggcgtagc gagcgaatac ctgcggatgc gttgcccagt
     3121 gttcgttgat ttgcgacgga aattcgacaa aatcacgcgg cgtgttggtg ccggaaagcg
     3181 tggcataacg ctggcgggca aaaaggccgt gcagcgtatg accaaattca tggaataagg
     3241 ttattacatc atcccagagt aacaacgcag gctcaccggc agcgggtttc tgataattgc
     3301 agacgttata aattaccgga tgcgtttcat taagcgttga ttgctcaaca aaattgccca
     3361 tccatgcacc gccgcttttt gaatcacggg cgaagaaatc accgtaaaat aacgccagcc
     3421 ccacgccatt atgatcaaaa atttcccaca cccgaacgtc aggatggtag acaggaatat
     3481 caaaacgttc gacaaactta ataccgaaga gctgattcgc ggtccagaat acaccttcat
     3541 tcaacaccgt gtttaattca aaatatggct tgagctgcgc ttcatcaaga tcaaatttct
     3601 cccgccgtac ctgttccgca taaaatgccc agtcccacgg ctgcgcgcta aacccgccct
     3661 gctgcttatc gataaccgcc tgtatggagg ctaattcatc gctcgcacgt tgacgcgccg
     3721 ctggaacaat ttcccgcata aagttgagtg ctgcttctgg cgtttttgcc atctgatcgg
     3781 cgattttcca tgcggcataa tgaggaaaac caagcagttt cgcctgctgt gcgcgaatct
     3841 ctaccagacg ttgaatgata gcgcgggtat cattgccatc atttttttcc gctcgcgtcc
     3901 agcccgcaat aaacagtttt tcacgcgtcg cacgatcgcg catttcggca agcgccggtt
     3961 gttgggtggt attcagcagc ggaatcagcc atttgttatc cagacctttc tcgcgagccg
     4021 cctctgccgc cagcgcaatc tcttgctcac tcattcctgc cagctgcgcg atatcgttca
     4081 caaccagacc gccggattta tttgctgcca gtaatcgctg gttaaactgg ctggtcaggg
     4141 tcgcagcttc tgtattcagt acttttaatt ttgctttatc agcttgcgca agtttggctc
     4201 cggcaaggac aaaacgttga tgaatcacct ccaccaggcg gatggattca ctatcaagcc
     4261 ccagggattc acggcgctgc cagacagcat ctacccgcgc gaataattca ccgttcagat
     4321 agatatcatt agccagttcc gccagttcag cggaaaactg ttcatcaaga cgctgtaatt
     4381 catcattggt atgcgccgca gtcatcgcaa aaaagacgct ggtaacgcgg gtaagtaatt
     4441 ctccgctttg ttccagtgcc agaatagtat tgttgaaatc aggcgtttgc gggttaagcg
     4501 cgatggcagc aatttctgcc cgcttttgct gcattccctc atcgaatgcc gggcgatagt
     4561 gatgattggc aatttgatca aaatggggag ccagatacgg tagtgtgctt tgcacaagga
     4621 aaggattcat tgttgtcatt ttcttctcct gaacgcgaga tgttccatag cgtaggctta
     4681 ctgataacgg agtgcaatct tgcaaaccag tattacccgc tcttaagcat cacgtgctat
     4741 gttattgaca cacaaaagcg ttgaggaaca gtgagatgat cgttttagta actggagcaa
     4801 cggcaggttt tggtgaatgc attactcgtc gttttattca acaagggcat aaagttatcg
     4861 ccactggccg tcgccaggag cggttgcagg agttaaaaga cgaactggga gataatctgt
     4921 atatcgccca actggacgtt cgcaaccgcg ccgctattga agagatgctg gcatcgcttc
     4981 ctgccgagtg gtgcaatatt gatatcctgg taaataatgc cggcctggcg ttgggcatgg
     5041 agcctgcgca taaagccagc gttgaagact gggaaacgat gattgatacc aacaacaaag
     5101 gcctggtata tatgacgcgc gccgtcttac cgggtatggt tgaacgtaat catggtcata
     5161 ttattaacat tggctcaacg gcaggtagct ggccgtatgc cggtggtaac gtttacggtg
     5221 cgacgaaagc gtttgttcgt cagtttagcc tgaatctgcg tacggacctg catggtacgg
     5281 cggtgcgcgt caccgacatc gaaccgggtc tggtgggtgg caccgagttt tccaatgtcc
     5341 gctttaaagg cgatgacggt aaagcggaaa aaacctatca aaataccgtt gcattgacgc
     5401 cagaagatgt cagcgaagcc gtctggtggg tgtcaacgct gcctgctcac gtcaatatca
     5461 ataccctgga aatgatgccg gttacccaaa gctatgccgg actgaatgtc caccgtcagt
     5521 aatttttata cccggcgtaa ctgccgggtt attgcttgtc acaaaaaagt ggtagactca
     5581 tgcagttaac tcactcacaa gcaagaacga atgaccgtcg aaacgcaact taatcccaca
     5641 cagcctgtca atcagcaaat ttatcgtatt cttcgtcgcg acattgtcca ttgcctgatt
     5701 gcaccaggca caccgttgtc ggaaaaagaa gtttctgttc gtttcaatgt gtcacgccag
     5761 ccggttcgtg aagcctttat taaactggcg gaaaacggcc tgattcaaat tcgtccgcag
     5821 cgtggcagct acgtcaacaa aatttccatg gcccaggtgc gcaatggcag ttttatccgt
     5881 caggccattg agtgcgcggt ggcgcgtcgg gcggcgagca tgattaccga aagccagtgc
     5941 taccaactgg aacaaaatct tcaccagcaa cgcattgcca ttgagcgcaa gcaactggat
     6001 gatttttttg aacttgatga caacttccat caactcctga cgcagattgc cgactgtcaa
     6061 ctggcgtggg ataccattga gaacctgaaa gcgaccgttg atcgcgtgcg ctatatgagt
     6121 ttcgaccacg tttctccacc agaaatgctg ttacgccagc atcttgatat tttctctgcc
     6181 ctgcaaaaac gtgatggcga tgcggtagaa cgtgcaatga cgcaacattt gcaggaaatc
     6241 agcgaatccg tgcgccagat ccgccaggaa aatagcgact ggtttagcga agagtaattc
     6301 atttcctctc atcccatccg gggtgagagt cttttccccc gccttatggc tcatgcatgt
     6361 atcaaaaaag atgtgagctt gatcaaaaac aaaaaatatt tcactcgaca ggagtattta
     6421 tattgcgccc gttacgtggg cttcgactgt aaatcagaaa ggagaaaata cctatgacga
     6481 cctacgatcg taaccgtaac gcaatcacca ctggcagccg tgttatggtt agcggcaccg
     6541 gtcacactgg caagatcctg tcgattgata ctgaaggtct gaccgctgag caaatccgcc
     6601 gcggaaaaac cgtagttgtt gaaggttgtg aagagaaact ggcacctctg gacctgattc
     6661 gtctcggcat gaactaagcg tgtgaatgcc gccgatggcg gcattgcttt ttttacttca
     6721 cggaatattt cgccacggtc gctttcgcgc catgcgctaa taaagacaag tacgcttccg
     6781 tcacttttgc agtaaacaaa ctattgtctg gcaaatcatc accaaagatc gccttaatcg
     6841 ccagcaatga ctggacgcgc gctttccctt cagcactact ttgtacagcc ttctgaataa
     6901 caggtaacag tgggtcactg atttcaatcg gatttccctg ttcatcaaca ccaccgacat
     6961 aacgcatcca acccgcgacg cccagtgcca gcagatcgaa cttgctgtca tgcgccagat
     7021 gccagcgaac agaatccaac atccgctgtg gcaatttctg gctgccatcc atcgcaatct
     7081 gccaggttcg atgacgtaac gccgggttgc tatagcgttc aattaatcgg ttagcgtaat
     7141 cttgcaaatc aacgccctgc actttcagcg tcggcgcttg ttcctgcaac atcaagccat
     7201 acgccgcata acgataatgt tcatcttcca tgcagtcatt aatgtgctga tatccagcaa
     7261 gataacccaa atacgccagg aatgaatgac tgccgttgag catgcgcaac ttcatctctt
     7321 cataaggcag cacatcacta accagttcgg ctcccgcttt ttcccattct ggacgtccgg
     7381 caacaaagtt atcttctatt acccactggc ggaaaggttc acaggcaacg cccgcaggat
     7441 cgcccacacc ggtaagttgt tcgattttcg ccagcgtatc ctctgtcact gcgggcacaa
     7501 tacggtccac cattgttgat gggaaagtca cgttatcttc gatccattgt gccagtttta
     7561 catcaacagc tttcgcgtat gaagtgacaa cgtcacgcat aacatgaccg ttttctggca
     7621 tgttgtcaca tgacatgacg gtaaatgcgg gaagtcctgc cgctttacgg cgggccagcg
     7681 cctcaacaat cacccctgtt gctgttttcg gctggtgggg attttgcacg tcggcagcga
     7741 ccatcgggtg atcgagcatt aactgtccgg tcgccggaga gtggaaatac cctttttcgg
     7801 tgattgtcag agagacaatc gcgatttgcg gttcacacat cgcagccaac acggtttcta
     7861 agccatccat ctgtacgtgc aaggctttct taacgacgcc aacgacgcga gccgtccacg
     7921 catcggccga catttccgca acggtataaa gattatcttg ctgttgtaaa tcggcaattt
     7981 gctgttcgcc gccgattaag ttgacctcat aatatcccca gtcactgaaa tgttctgtag
     8041 caagaatatc ggcatacacg ccctgatgcg cacggtgaaa tgcaccaaag cctaaatgaa
     8101 taattcttgg ggccaggtta ttacgatcat aaacagggag tgtcgctttt gctgataaca
     8161 aattatttcc cataacaatt ccttaaatat aaatatggca aactatatgt tttgtta
//
LOCUS       DAETQN010000167         8182 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.169, whole genome shotgun sequence.
ACCESSION   DAETQN010000167 DAETQN010000000
VERSION     DAETQN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.169"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="K0W68_003907"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="K0W68_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBI3075338.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /locus_tag="K0W68_003908"
     CDS             complement(2975..3697)
                     /locus_tag="K0W68_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBI3075339.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="K0W68_003909"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="K0W68_003909"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBI3075340.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYGTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="K0W68_003910"
     CDS             6679..>8182
                     /locus_tag="K0W68_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HBI3075341.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINSQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1978 a         2029 c         2322 g         1853 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact tggcggttta tggcacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 tcacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAETQN010000168         8155 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.170, whole genome shotgun sequence.
ACCESSION   DAETQN010000168 DAETQN010000000
VERSION     DAETQN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.170"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..449)
                     /locus_tag="K0W68_003911"
     CDS             complement(<1..449)
                     /locus_tag="K0W68_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBI3075342.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFN"
     gene            complement(566..1612)
                     /gene="ansB"
                     /locus_tag="K0W68_003912"
     CDS             complement(566..1612)
                     /gene="ansB"
                     /locus_tag="K0W68_003912"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBI3075343.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1788..2507)
                     /locus_tag="K0W68_003913"
     CDS             complement(1788..2507)
                     /locus_tag="K0W68_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBI3075344.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2691..3017)
                     /locus_tag="K0W68_003914"
     CDS             complement(2691..3017)
                     /locus_tag="K0W68_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBI3075345.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="K0W68_003915"
     CDS             complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="K0W68_003915"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBI3075346.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3897..4949
                     /gene="mutY"
                     /locus_tag="K0W68_003916"
     CDS             3897..4949
                     /gene="mutY"
                     /locus_tag="K0W68_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBI3075347.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4977..5252
                     /locus_tag="K0W68_003917"
     CDS             4977..5252
                     /locus_tag="K0W68_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBI3075348.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5317..6396
                     /gene="mltC"
                     /locus_tag="K0W68_003918"
     CDS             5317..6396
                     /gene="mltC"
                     /locus_tag="K0W68_003918"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBI3075349.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6598..7854
                     /gene="nupG"
                     /locus_tag="K0W68_003919"
     CDS             6598..7854
                     /gene="nupG"
                     /locus_tag="K0W68_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBI3075350.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7903..>8155)
                     /locus_tag="K0W68_003920"
     CDS             complement(7903..>8155)
                     /locus_tag="K0W68_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBI3075351.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1922 a         2159 c         1987 g         2087 t
ORIGIN      
        1 taattgaaat cttcgctatt cacgatcaga tatttattat tcgttttgat atcgtcgacc
       61 acccgcccac gatcgctggc tgccgcctga aaagagtaaa agttcaccga ccagccagac
      121 gcgggcgttc ctttactggt taacatcgcc gagaatttta ccggtttatc tttcgtccag
      181 gtctgttcga gcagttcgta atctgccaac tgaccgaccc aggtatagag atcgtcactg
      241 gaagagacat cgccttccgc tgaccaggtg gcctgattac ccttagcatc aagatttttt
      301 aaggtgatag catcaagctt gagaatgccg tgatactgct ttttgaattg atcttttaag
      361 atattttcag ttggcggttc gccatctgcc caggcattac cagtcatcaa cataagcagt
      421 gccgtgccaa caatccattg ctttttcacc cgaaaaacct cacgcggaat aatttattcc
      481 ggtgattatc attagggtaa tgaaaattgg caatggacgt aagtctgaag tgaaaaagcc
      541 ccggcacgat accggggcga ggtgattagt actgattgaa gatctgctgg atctgctgcg
      601 gatctttggt ttgcgtcaga gccagctgca gcaggacgcg cgctttttgc gggttcagcg
      661 tgccagaggc gacgaagccg tatttcgcat catccacttc agcatcctga gtggtagcac
      721 ccgtcggtac gcgggaagaa cgcactactg cagtgccgtt tttcgcggcg gttgccaggg
      781 tgtcgaacac ggatttatac aggttaccat tacccacacc agcgctaacg atgccatcat
      841 agcccgcatc taccagtgct ttagccggaa gatcggatgc gttagcgtag ttataaacga
      901 tgccgacttt cggcagctca ttcagcttag agacatcgaa tggcgtatcg ctggtgtgct
      961 tacgtgccgg ggtacgttgg tagtcaatct taccgttgtg aatgtatccc agaggaccgt
     1021 agttaacaga cttgaaggtc gctacgtcgg tggtgttggt tttggtgaca tcgcgaccgt
     1081 ccagtacggt gtcgttcatc accaccagca cgccacgatt agcggaggct ttatcagctg
     1141 cggtcactac cgcgttatac aggttgaatg gaccgtctgc gctcatggac gtggacgggc
     1201 gcattgcgcc gaccatcacc accggtttgt cgcatttcac cgtcaggtcg aggaagtaag
     1261 cggtttcttc catcgtgtcg gtaccgtggg taatgacgaa gccgtcggtt ttatcgcagt
     1321 cggtgttaat ttttttcgcc agtgtcagcc agacattatc gttcatgtcc tgggagccga
     1381 tattcactac ctgctcgcct ttaacgttcg caatgtcttt tagttgcggc accgcattaa
     1441 ccagattttc tacgccaact ttacccgctg tgtagttaga tttggttgcg gagtcaccac
     1501 caccggcaat ggtcccgccg gttgctaaaa tggtgatatt gggtaatgcc aatgctgcac
     1561 cactaaaacc cataaccagt gcggcaagtg ccgtcttttt gaaaaactcc atttcattcc
     1621 tccagttacg tgaacgctac gcattatccc ttagctctgt atgggaaatt tgacgttaaa
     1681 caatttacaa cgtgaatata ttttggagat ctacaaagtt agaggcaggt aacaaaacga
     1741 agaattaaac ggcataaaaa agtattatgc cgtcttaaaa tagaggatta ttttaaatta
     1801 ccgaccaggg ctttgcggct atcttccaga gtcacaacgc ggctacaaac atctttgcca
     1861 aactgctgga aatctttttc ctgctttttc cactctgttt ggattgagga ttgcagcccc
     1921 cccaggcttc ccagcacatt ctgtaatggg ttaccgccgc ttttcagcac cgctttcgcg
     1981 cccatttcat taatgctgtc ctgtaaaatt ccacccattg cctgattcac taattgctgg
     2041 ccttcggcgc gaacctgatc aatggcttta tagtgaaacg tcaggccatc gctgcgcgtt
     2101 tcgataatgc ggttcatctg ctctttcagc tgcgcatcaa gtttggtcag acggctgcgc
     2161 attttgctgc tttcgcccat ctcctgaacg ataattttat ccagcgcaat acgggctttt
     2221 tcgacgcggc ttttcgcgcc ttcatcaatc cacggcagcg tgctgcgtag ttcagcctga
     2281 taatccttcg cctgctcgcg ctgggcggca ttcagggaat attgcttacc gttatacatc
     2341 acgttgccgt ctggcgtgat caccagattg ccgttttcgc ccttcacctg cacggtttgc
     2401 gggctgacaa tcacatcgtc acgcggcgtg acgctgcact ggtagtcggc gtgagcggtc
     2461 attgccgtca ctgaaagtgc tgccgccagc agcattttgc gcatcatagt cttccctcaa
     2521 gaaaaaatca ggccagcatt tgctggcccc agattgataa caaagtacgc gttttccatg
     2581 ccggatgcgg cgtaaacacc ttatcccgcc tactcgatcg tgcatctttc caggcctgat
     2641 aagcgtagcg catcaggcag ttttgcattt gtcatcgccc gtatgctttc ttagtcccac
     2701 caaacgtcga aaagttcgct ggtgcgtacc tcatccagtt tgcgctcttc cagccactta
     2761 cgcacaatcg cctgatgttc ttcggtgcat ttgccgattt cctgcatgca gatcagacct
     2821 tcccaggcca gataaccgct gccgtcaaag gccagtttgt tcggttcgat aacctcgtta
     2881 ataaaatcat caacggtttt atcaatctgt tcttccgatg taccttccgg gaatcgccat
     2941 gccaccgaaa atcctaattc ctggaattcg tcgatgtgca tttttttacg cagacgacgg
     3001 ctacggttct ttgccattat ttcaccctct cgaacattaa gtcccatact ccgtgaccaa
     3061 gacgatgacc acgttgttca aatttcgtca ccggacgtga tgccggacgc ggcacgtaat
     3121 cattgctctc tgacaggttt ttataaccgt caatagaaga catcacttca agcatatgtt
     3181 ccgcataagg ttcccagtcg gtcgccatat ggaatacacc ccccagctgc agtttgcttt
     3241 ttaccagttc ggcaaacggc acctgtacga tacggcgttt attatggcgc gctttgtgcc
     3301 acgggtcagg gaaaaagagc tgcaccatgc gcaatgaatt gtcaggaatc attttatgca
     3361 gcacttcaac cgcatcgtga cacatcacgc gcaggttgct taaaccttct tcatgcgcag
     3421 aagccaggca cgcaccaacg cccggtgaat gcacttcaat gccgaggaag tcctgctcag
     3481 ggcgatcttt agccattgcc accagcgacg cccccatgcc aaaaccaatc tcaagcgtca
     3541 ccggcgcttc acggccaaaa agcgcgggga aatccagcat atcttcgctg aactcaacgc
     3601 ccatcaccgg ccagtagttt tccagcgcat gttcctggcc tttggtcagt cgcccctggc
     3661 ggcgcacaaa actacggata cggcgcagtg ggcggccgtt ttcatcaaat tccggtgaaa
     3721 tgacgtcgtt tttcataaag atttagtcgc ttgtgaaagt gttctgaaaa cgggcattat
     3781 ccaaagttag ttgccggatg caagcatgat aaggccgtgg ctgcggaaag ttccggttta
     3841 caccctgccg tcgctgtgct gcaatcttgc ccccaacaac agtgaattcg gtgaccatgc
     3901 aagcgtcgca attttcagcc caggttctgg actggtacga taaatacggg cgaaaaacgc
     3961 tgccctggca aattgacaag acgccctaca aagtatggct ctcagaagtg atgttgcaac
     4021 aaactcaggt tgcgaccgtt atcccctatt ttgaacgctt tatggcgcgc ttcccgacgg
     4081 tgaccgatct cgccaatgcg ccgctcgacg aagttctcca cttgtggacc gggcttggct
     4141 attacgcccg cgcgcgcaat ctgcataaag cggcacaaca agtggcgacc ttacacggcg
     4201 gtaaattccc ggaaaccttt gaagaagtcg cggcgttacc gggcgtcggg cgttccaccg
     4261 caggcgcgat tctctcgctt tctctgggta agcactttcc gattctcgac ggtaacgtca
     4321 aacgcgtgct ggcgcgctgc tatgctgtaa gcggctggcc tgggaaaaaa gaggtcgaga
     4381 ataaattatg gagtttgagc gagcaggtga cgcccgcggt cggcgtggaa cggtttaatc
     4441 aggcgatgat ggatttgggc gcgatgattt gtacgcgctc gaagccgaaa tgttcgctct
     4501 gtccgctaca aaacggatgt attgccgccg ccaataatag ctggtcgctt tatccgggca
     4561 aaaaaacgaa acagacgctg ccggagcgca ccggctactt tttgctgtta cagcacgaag
     4621 atgaagtatt gctggcgcag cgtccgccga gcggattgtg gggcggttta tactgtttcc
     4681 cgcagtttgc cgacgaagaa agtttgcggc agtggctggc gcaacggcag attgctgccg
     4741 ataacctgac gcaactgacc gcgtttcggc ataccttcag ccatttccac ttagatattg
     4801 tgcctatgtg gcttcccgtg tcgtcattca ccggctgcat ggatgaaggc aatgcgctct
     4861 ggtataactt agcgcaaccg ccgtcagttg gcctggcggc tcccgtggag cgtttgttac
     4921 agcagttacg cactggcgcg ccggtttagc gcgtgagtcg ataaagagga tgatttatga
     4981 gcagaacgat tttttgtact ttcctgcaac gtgaagcaga aggtcaggat tttcagctgt
     5041 accccggcga gctgggaaaa cgcatctata acgagatctc caaagaagcc tgggcgcagt
     5101 ggcagcacaa gcaaaccatg ctgattaatg aaaagaaact caacatgatg aatgccgagc
     5161 accgcaagct gcttgagcag gagatggtca acttcctgtt cgagggtaaa gaggtgcata
     5221 tcgagggcta tacgccggaa gataaaaaat aaaaacagtg ccggagcacg cctccggcaa
     5281 cttgcataaa aacaaacaca acacgcaccc ggaatgatga aaaaatatct cgcgctggct
     5341 ttgattgcgc cgttgctcat ctcctgttcg acgaccaaaa aaggcgatac ctataacgaa
     5401 gcctgggtca aagataccaa cggttttgat attctgatgg ggcaatttgc ccacaatatt
     5461 gagaacatct ggggattcaa agaggtggtg atcgccggtc ctaaggacta cgtgaaatac
     5521 accgatcaat atcagacccg cagccacatc aacttcgatg acggtacgat tactatcgaa
     5581 actatcgccg ggacagaacc tgccgcgcat ttgcgccggg caattatcaa aacgttgctg
     5641 atgggtgacg atccgagttc ggtcgatctc tattccgacg ttgatgatat tacgatttcg
     5701 aaagaacctt tcctttacgg tcaggtggtg gacaacaccg ggcagccgat tcgctgggaa
     5761 ggtcgcgcaa gcaacttcgc ggattatctg ctgaaaaacc gtctgcaaag ccgcagtaac
     5821 gggctgcgta tcatctacag cgtcaccatt aacatggtac cgaaccacct tgataaacgt
     5881 gcgcacaaat atctcggcat ggtccgccag gcgtcacgga aatatggcgt tgatgagtcg
     5941 ctgattctgg caattatgca gaccgaatct tcctttaacc cgtatgcggt cagccgttcc
     6001 gatgcgctgg gattaatgca ggtggtacaa catactgccg ggaaagatgt gttccgctcg
     6061 caggggaaat ccggcacgcc gagccgcagt ttcttgtttg atcctgccag caatattgat
     6121 accggcaccg cgtatctggc gatgctgaac aatgtttatc tcgacggaat tgataaccca
     6181 acatcgcggc gttatgccgt catcaccgcc tataacggcg gcgcaggcag cgtgctgcga
     6241 gtcttttcga atgataagat tcaggctgcc aatattatta acaccatgac gccgggcgat
     6301 gtttatcaga cgctgacgac ccgccatccc tctgcggaat ctcgccgtta tctttataaa
     6361 gtgaataccg cgcaaaaatc ctaccgccgc cgataattcc attaaccgcc cctgacgatg
     6421 ctcaggggca aaaatgttat ccacatcaca atttcgtttt gcaaattggg aatgtttgca
     6481 attatttgcc acaggtaaca aaaaaccagt ccgcgaagtt gatagaatcc catcatctcg
     6541 cacggtcaaa tgtgcttttt caaacactca tccgcatcac aatgtgagga aattaacatg
     6601 aatcttaagc tgcagctgaa aatcctctct tttctgcagt tctgtctgtg gggaagttgg
     6661 ctgacgaccc tcggctccta tatgtttgtt accctgaagt ttgacggtgc ttctattggc
     6721 gcagtttata gctcactggg tatcgccgcg gtatttatgc ctgcgctgct ggggattgtg
     6781 gccgacaaat ggttaagtgc gaaatgggta tatgccattt gccacaccat tggcgctatc
     6841 acgctgttca tggcggcaca ggtcacgacg ccggaagcga tgttccttgt gatattgatt
     6901 aactcgtttg cttatatgcc aacgcttggg ttaatcaaca ccatctctta ctatcgcctg
     6961 caaaatgccg ggatggatat cgttactgac ttcccgccaa tccgtatctg gggcaccatc
     7021 ggctttatca tggcaatgtg ggtggtgagc ctgtctggct tcgaattaag ccacatgcag
     7081 ctgtatattg gcgcagcact ttccgccatt ctggttctgt ttaccctgac tctgccgcat
     7141 attccggttg ctaaacagca agcgaatcag agctggacaa ccctgctggg cctcgatgca
     7201 ttcgcgctgt ttaaaaacaa gcgtatggca atcttcttca tcttctcaat gctgctgggc
     7261 gcggaactgc agattaccaa catgttcggt aataccttcc tgcacagctt cgacaaagat
     7321 ccgatgtttg ccagcagctt catcgtgcag catgcgtcaa tcatcatgtc gatttcgcag
     7381 atctctgaaa ccctgttcat tctgaccatc ccgttcttct taagccgcta cggtattaag
     7441 aacgtaatga tgatcagtat tgtggcgtgg atcctgcgtt ttgcgctgtt tgcttacggc
     7501 gacccgactc cgttcggtac tgtactgctg gtactgtcga tgatcgttta cggttgcgca
     7561 ttcgacttct tcaacatctc tggttcggtg tttgtcgaaa aagaagttag cccggcaatt
     7621 cgcgccagtg cacaagggat gttcctgatg atgactaacg gcttcggctg tatcctcggc
     7681 ggcatcgtga gcggtaaagt tgttgagatg tacacccaaa acggcattac cgactggcag
     7741 accgtatggt tgattttcgc tggttattcc gtggttctgg ccttcgcgtt catggcgatg
     7801 ttcaaatata aacacgttcg tgtcccgaca ggcacacaga cggttagcca ctaattacgc
     7861 aaagaaaaac gggtcgccag aaggtgaccc gtttttttat tcttacttca acacataacc
     7921 gtacaatcgt ttcatgccat ccgcatcggt ttcgctatag acaccttgca actccggcga
     7981 aaatcccggc aacaaattca cgccttcttc cagcgcaagg aaataacgtt gaaccgcccc
     8041 accccagact tccccgggaa ccacgcaaag cacgccaggt ggataaggca acgccccttc
     8101 tgccgcaatt cgcccttcgg catcacgaat ccgcaccaac tccacttcac cgcga
//
LOCUS       DAETQN010000169         8127 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.171, whole genome shotgun sequence.
ACCESSION   DAETQN010000169 DAETQN010000000
VERSION     DAETQN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8127
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.171"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            167..321
                     /locus_tag="K0W68_003921"
                     /pseudo
     CDS             167..321
                     /locus_tag="K0W68_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            367..783
                     /gene="hicB"
                     /locus_tag="K0W68_003922"
     CDS             367..783
                     /gene="hicB"
                     /locus_tag="K0W68_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HBI3075352.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            862..2268
                     /locus_tag="K0W68_003923"
     CDS             862..2268
                     /locus_tag="K0W68_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBI3075353.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2513..3658
                     /locus_tag="K0W68_003924"
     CDS             2513..3658
                     /locus_tag="K0W68_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBI3075354.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3676..4689
                     /locus_tag="K0W68_003925"
     CDS             3676..4689
                     /locus_tag="K0W68_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3075355.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4690..5590
                     /locus_tag="K0W68_003926"
                     /pseudo
     CDS             4690..5590
                     /locus_tag="K0W68_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5580..6374
                     /locus_tag="K0W68_003927"
     CDS             5580..6374
                     /locus_tag="K0W68_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3075356.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            6396..7820
                     /gene="patD"
                     /locus_tag="K0W68_003928"
     CDS             6396..7820
                     /gene="patD"
                     /locus_tag="K0W68_003928"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBI3075357.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            8030..>8127
                     /locus_tag="K0W68_003929"
     CDS             8030..>8127
                     /locus_tag="K0W68_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HBI3075358.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNK"
BASE COUNT         1921 a         1908 c         2222 g         2076 t
ORIGIN      
        1 attattcagt tttgcaaatt agcgcaaaga aattctggaa tcttccttcc tgattttgca
       61 ttgcattctg ccgttgcggc gatttagtgc tattttcgag caaattacac ataggggtaa
      121 acactaatgt ttactttatt gatatactca gcggcaggga ggcgatgtga aacaaagcga
      181 gttcagacgt tggctcgaat ctcagggcgt cgatgtagcg aatggccgca accatttgaa
      241 actcaggttt catgggaggc gcagtgtcat gccgcgtcac tcctgcgtaa agcaatcctg
      301 aaacaactcg gtttgagtta atcgccaatt aaaaaggtta atgacatgcg agagacagtc
      361 gaaattatgc gttatcccgt cactcttaca cccgcgccgg aaggcggtta tatggtttct
      421 tttgtggata tccctgaagc gttgacgcag ggcgaaactg tcgctgaagc gatggaagcg
      481 gcaaaagatg ctttactgac cgcatttgat ttttattttg aagataacga gcttatcccg
      541 ttaccttcgc cattaaatag tcatgatcac tttattgaag tacctttgag cgtcgcctct
      601 aaagtattgc tgttaaatgc ttttttacag tcagaaatca ctcagcaaga gttagccagg
      661 cgaattggca aacctaaaca ggagattact cgcctattta acttgcacca tgcgacaaaa
      721 atcgacgccg tccagcttgc ggcaaaggcg cttggcaaag agttatcgct ggtgatggtt
      781 taattacagt taacgaaaag ttgtcatttt taacaactga tatagactgc cgaatcatct
      841 gcacataatt acgattcgat aatgaaaaaa taccagcagc ttgcagaaca attacgcgag
      901 cagattgcgt cgggtatctg gcaacccggc gatcgtttgc cttcgttgcg tgaccaggtg
      961 gcgctttcag gcatgagctt tatgactgtc agccatgcct atcagttgct cgaaagtcag
     1021 ggatatatta tcgcacgacc gcagtcgggt tattacgttg cgccacaggc aataaaaatg
     1081 ccgaaagcgc cagtcattcc agtcactcga gatgaagcag tcgatatcaa cacttatatt
     1141 tttgatatgt tgcaggccag tcgcgaaccg tcggtcgttc cgtttgcctc ggcctttccc
     1201 gacccgcgac ttttccccct ccaacaacta aaccgctcgc tggcgcaggt aagcaaaacc
     1261 gccacagcga tgagcgtgat tgaaaactta ccgccaggaa acgcagaact gcgtcaggct
     1321 attgctcgtc gctatgcctt acagggcatc accatttctc ctgatgaaat tgtcattact
     1381 gccggggcgt tagaggcatt aaacctcagt ttgcaagcgg taactgaacc gggcgattgg
     1441 gtgatagtag agaatccttg tttctacggt gcgttgcagg cgctggagcg gctacggctg
     1501 aaggcgttat cggtggcgac ggatgttaaa gaagggatcg atcttcaggc gctggaactg
     1561 gcgttgcagg attatccggt gaaagcgtgc tggctgatga ctaatagcca gaacccactc
     1621 ggatttacct taacgccgca aaaaaaagca caactggtgg cgttactcaa tcagtacaac
     1681 gtaacgctga ttgaagatga cgtttacagc gaactttatt ttggacggga aaaaccgctg
     1741 cctgcgaaag cgtgggatcg ccacgatggc gttttgcatt gctcttcgtt ttcgaaatgt
     1801 ctggtgcctg gttttcgtat tggttgggtc gccgccggaa aacatgcacg taaaattcaa
     1861 cgcttgcagt tgatgagtac gctttccacc agctcaccga tgcaacttgc gctggtggat
     1921 tacctttcca cgcgccgata cgacgcccat cttcgtcgcc tgcgtcgcca gcttgcggaa
     1981 cgtaaacaac gtgcctggca ggcactgctg cgttatctgc ctgcggaagt taaaattcat
     2041 catagtgaca gcggttattt tctctggctg gagctccccg agccgttaga tgccggcgaa
     2101 ttaagcctgg cggcactgac gcatcatatc agtattgcgc cgggtaaaat gttttctacc
     2161 ggtgaaaact ggtcacgttt ttttcgtttt aataccgcgt ggcagtgggg agaacgtgaa
     2221 gaacaggcgg taaaacaatt aggcaaactt attcaagaac ggctgtaata gcgtttaatt
     2281 taattcctct tagattgggt aatatgaatt tcgaatagca gtcatatttc ctaacccctt
     2341 gtctatactc cagaagataa ccttacagac ggcataatgc gcggtagctc acaacctgat
     2401 taaattttct cagggacgaa ggtgtgcctg caagccgccg tctatggtta aacaaggaga
     2461 tatttttacg gcacggcggc tgaacaatta attacgacag gagtaagacc ttatgagcaa
     2521 gacatttgcc cgcagcagcc tgtgtgcgct caccatgaca ataatgaccg ctcacgccgc
     2581 cgaaccacct accaatttag ataaaccgga agggcgactg gatattatcg cctggccggg
     2641 atacatcgaa cgcggacaaa ctgataaaca atacgactgg gtaacgcaat ttgaaaaaga
     2701 gacaggctgc gcggtgaatg tgaaaaccgc cgcgacttcc gatgaaatgg tcagtctgat
     2761 gaccaaaggg ggttacgatc tggttacggc atccggcgat gcctcgctgc gtttgattat
     2821 gggcaaacgc gtgcagccga ttaataccgc cttgattccc aactggaaaa cgctcgatcc
     2881 gcgcgtggtt aaaggcgact ggtttaatgt tggcggcaaa gtttacggca caccttacca
     2941 atgggggccg aacctgctga tgtacaacac taaaaccttc ccgacgccgc cggatagctg
     3001 gcaagtggtt tttgttgagc aaaatctgcc ggacggcaag agcaataaag gccgcgttca
     3061 ggcttatgat ggccctatct acattgcgga cgctgcgttg ttcgttaaag ccactcagcc
     3121 gcagttgggc atcagcgatc cgtatcaact aaccgaagaa cagtaccagg cggtgctgaa
     3181 agtgctgcgc gctcaacaca gtttgatcca tcgctactgg catgacacta ccgtgcaaat
     3241 gagcgatttc aaaaacgagg gtgtggttgc ttccagtgcc tggccctatc aggccaacgc
     3301 cctgaaagcc gaaggccagc ctgttgctac cgttttcccg aaggagggtg ttaccggttg
     3361 ggctgatacc accatgctgc atagcgaagc gaaacatccg gtttgcgcct acaaatggat
     3421 gaactggtca ttaacgccaa aagtgcaggg cgatgtggcg gcctggtttg gctcgttacc
     3481 ggtagtgccg gaagggtgta aagccagtcc gttattaggc gaaaaaggtt gtgaaaccaa
     3541 cggttttaac tatttcgaca aaatcgcctt ctggaaaacg cctatagcag aagggggcaa
     3601 gtttgttccc tacagtcgct ggacgcagga ttacattgcc attatgggcg gtcgctaact
     3661 tcgctggggt gctttatgac gtacgcagtg gagtttgaca acgtctcgcg gttgtacggt
     3721 gacgtgcgcg cagtagatgg cgtcagtatt gcgataaaag atggtgagtt cttctctatg
     3781 ctggggccgt ccggctccgg caaaaccacc tgcctgcgcc tgattgctgg cttcgaacag
     3841 ctttccggcg gggctatctc tatctttggt aaacccgcca gcaatctgcc accgtgggag
     3901 cgggacgtga atactgtctt tcaggactac gcgctatttc cgcatatgtc gattcttgac
     3961 aatgtcgcct atgggctgat ggtcaaaggc gtgaataaaa agcagcggca cgcaatggcg
     4021 caagaggcgc tggagaaagt ggcgttgggg tttgtacagc aacgtaaacc gtcacaactt
     4081 tctggtggtc agcgccagcg ggttgctatc gccagagcat tggtgaatga accgcgcgta
     4141 ttgctgttgg atgaaccgct cggcgcactg gatctcaaat tgcgtgagca gatgcagctg
     4201 gaactgaaaa aactgcaaca gtctctcggt atcactttta tcttcgttac ccacgatcag
     4261 ggcgaagcgt tatcgatgtc cgatcgtgtg gcggttttca ataatggacg cattgagcag
     4321 gtcgattccc cgcgcgatct ctatatgcgc ccgcgcacgc cgtttgttgc cgggttcgtt
     4381 ggtacatcga atgtttttga tggactgatg gcagagaaac tttgtggcat gacgggaagc
     4441 ttcgccctgc gaccggaaca tatccgcctc aacacccctg gtgaactgca ggccaatggc
     4501 acgatccagg cggtgcaata tcagggcgcg gcaactcgtt ttgaactgaa attgaacggc
     4561 ggtgaaaaac tgcttgtgag tcaggccaat atgacaggcg aagaactgcc tgccacgctc
     4621 acgcccggac aacaggtgat ggtttcctgg tcgcgtgatg tgatggtgcc gctggttgag
     4681 gagaggtgaa tggcgatgaa tgtattgcaa tcaccttcac gtccaggtct ggggctgttt
     4741 ttactgctgc ttggcccgct aatgtggttt ggcattgtct atttcggctc gctgctgaca
     4801 ctgttatggc agggatttta tacttttgac gatttcacca tgtcggtaac gccggaactg
     4861 acgctggcga atatccgtgc gctgtttaat ccggcgaatt acgacatcat tctccgcacg
     4921 ctgactatgg ctgtggcagt cactatcgcc agcgccattc tggcttttcc aatggcgtgg
     4981 tatatggcgc gctataccag cgggaaaatg aaagcgtttt tatattgcgg taatgttgcc
     5041 gatgtgggcg agctacattg ttaaagccta tgcctggacg ttattgctgg caaaagatgg
     5101 cgtggctcag tggtttttac aacatcttgg gctggaacca ctgctgactg cgttccttac
     5161 attacctgcg gtgggcggaa atacgctgtc aacttccggg ctggggcgct ttctggtgtt
     5221 tctctatatc tggttgccgt tcatgatcct gcccgttcag gcggcgcttg agcgtttgcc
     5281 gccgtcattg ttgcaggcgt cggctgatct cggcgcacgt ccacgacaaa cctttcgcta
     5341 tgtggtgctg ccgctggcaa tcccgggtat tgccgctggc tctatcttta ccttctcact
     5401 cactctgggc gattttatcg tcccgcagct ggttggtcct ccaggatatt ttatcggcaa
     5461 tatggtttat tcccagcagg gggcgattgg caatatgccg atggcggcgg cattcacctt
     5521 ggtgccgatt attctcatcg cactgtacct ggcgttcgtg aaacgtctgg gagcgttcga
     5581 tgcactctga acgcgcaccg tttttcctca aactggcggc ctggggcggc gttgttttcc
     5641 tacattttcc catcctgata atcgccgcct atgcgtttaa cactgaagat gcggcgttta
     5701 gttttccacc gcagggcctg acgctgcgct ggtttagcgt ggcagcacag cgtagtgata
     5761 ttcttgatgc cgtgacactg tcacttaaag tggcggcgct ggcgacatta attgcgctgg
     5821 tgttagggac gctggcagct gccgcgctgt ggcgacgaga ctttttcggc aaaaacgcca
     5881 tttcgctgtt actgctgctg cccattgcgc tgccgggcag cgtcactggt ctggcgttat
     5941 taaccgcctt taaaaccatc aatctggagc cgggattttt caccatcgtg gtcggtcatg
     6001 cgactttttg tgtagttgtg gtgtttaaca atgtcatcgc ccgttttcgc cgcacctcct
     6061 ggagtctggt tgaggcgtca atggatcttg gggccaatgg ctggcaaacc ttccgctacg
     6121 tagtgttgcc gaatctcagt tcggcgttac tggcaggagg aatgctggcg tttgccttgt
     6181 cgttcgatga aatcatcgtt acgaccttta cggcaggtca tgaacgaacg ttactgttgt
     6241 ggttgctcaa tcagcttggg cgaccgcgtg atgtaccggt aactaacgtg gtggcactgc
     6301 tggttatgtt ggtaacaacc ttgccgatcc tgggggcctg gtggctaacc cgcgaaggcg
     6361 acaatggtca ataaccactg atacaggaat atgctatgca acataagtta ctgattaacg
     6421 gagaactggt tagcggcgaa ggggaaaaac agcctgtcta taatccggca acgggggacg
     6481 ttttactgga aattgccgag gcatccgcag agcaggtcga tgctgctgtg cgcgcggcag
     6541 atgcagcatt tgccgaatgg gggcaaacca cgccgaaagt gcgtgcggaa tgtctgctga
     6601 aactggctga tgttatcgaa gaaaatggtc aggtttttgc cgaactggag tcccgtaatt
     6661 gtggcaaacc gctgcatagt gcgttcaatg atgaaatccc ggcgattgtc gatgtttttc
     6721 gctttttcgc gggtgcggcg cgctgtctga atggtctggc ggcaggtgaa tatcttgaag
     6781 gtcatacttc gatgatccgt cgcgatccgt tgggggtcgt ggcttctatc gcaccgtgga
     6841 attatccgct gatgatggcc gcgtggaaac ttgctccggc gctggcggca gggaactgcg
     6901 tagtgcttaa accatcagaa attaccccgc tgaccgcgtt gaagttggca gagctggcga
     6961 aagatatctt cccggcaggc gtgattaaca tactgtttgg cagaggcaaa acggtgggtg
     7021 atccgctgac cggtcatccc aaagtgcgga tggtgtcgct gacgggctct atcgccaccg
     7081 gcgagcacat catcagccat gccgcgtcgt ccattaagcg tactcatatg gaacttggtg
     7141 gcaaagcgcc agtgattgtt tttgatgatg cggatattga agcagtggtc gaaggtgtac
     7201 gtacatttgg ctattacaat gctggacagg attgtactgc ggcttgtcgg atctacgcgc
     7261 aaaaaggcat ttacgatacg ctggtggaaa aactgggtgc tgcggtggca acgttaaaat
     7321 ctggtgcgcc ggatgacgag tctacggagc ttggaccttt aagctcgctg gcgcatctcg
     7381 aacgcgtcag caaggcagta gaagaggcga aagcgacagg gcacatcaaa gtgatcactg
     7441 gcggtgaaaa gcgcaagggt aatggctatt actatgcgcc gacgctgctg gctggcgcat
     7501 tacaggacga tgccatcgtg caaaaagagg tatttggtcc agtagtgagt gttacgccct
     7561 tcgacaacga agaacaggtg gtgaactggg cgaatgacag ccagtacgga cttgcatctt
     7621 cggtatggac gaaagatgtg ggcagggcgc atcgcgtcag cgcacggctg caatatggtt
     7681 gtacctgggt caatacccat ttcatgctgg taagtgaaat gccgcacggt gggcagaaac
     7741 tttctggtta cggcaaggat atgtcacttt atgggctgga ggattacacc gtcgtccgcc
     7801 acgtcatggt taaacattaa gttagatgtt agtggctgtt ataggacggc cactatcgtt
     7861 ttaggttctg agtttaatgg cggttaagta ttagaatgat tacattaata actagcgtaa
     7921 aaattaggct aaatgtttga tggcgatttt tcatcctgtc acagttgatt agcataaggc
     7981 tttagagtat cgttattaaa ctaataattt ataagtagaa gtattaacaa tgaaagttca
     8041 attgtacaaa ttcacggaag ataaaaataa aacccttacc ttcagatgga cgaaaaaaca
     8101 ctttgagttt tgtatggata ataaaat
//
LOCUS       DAETQN010000170         8104 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.172, whole genome shotgun sequence.
ACCESSION   DAETQN010000170 DAETQN010000000
VERSION     DAETQN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.172"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(101..796)
                     /locus_tag="K0W68_003930"
     CDS             complement(101..796)
                     /locus_tag="K0W68_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HBI3075359.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(789..2216)
                     /locus_tag="K0W68_003931"
     CDS             complement(789..2216)
                     /locus_tag="K0W68_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HBI3075360.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2227..2946)
                     /locus_tag="K0W68_003932"
     CDS             complement(2227..2946)
                     /locus_tag="K0W68_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HBI3075361.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="K0W68_003933"
     CDS             complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="K0W68_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HBI3075362.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            4554..5879
                     /locus_tag="K0W68_003934"
     CDS             4554..5879
                     /locus_tag="K0W68_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HBI3075363.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            5988..6224
                     /locus_tag="K0W68_003935"
     CDS             5988..6224
                     /locus_tag="K0W68_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI3075364.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6236..6829
                     /gene="rclC"
                     /locus_tag="K0W68_003936"
     CDS             6236..6829
                     /gene="rclC"
                     /locus_tag="K0W68_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HBI3075365.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(6989..7858)
                     /locus_tag="K0W68_003937"
     CDS             complement(6989..7858)
                     /locus_tag="K0W68_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBI3075366.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2193 a         1982 c         1732 g         2197 t
ORIGIN      
        1 agcataacca tgacggaacg gggttaagtc aggttgtcct taacgctatt ttcaacctcg
       61 tatgccttct tcaggtttat gttcagatct catatctctc tcagcaatcc tcaataatca
      121 gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca atatccgccg
      181 ttgagctggg accactgatg atgttaatac atgacggcat tcgttcaccg gcctgcgctt
      241 tctgatgcaa tttttctgcg agttgcgcta cacgcggcag gattgtgctt ttacgcagga
      301 taaaaagaga agattccggg agcaggctca atgaacgccc gcgctcgacg gcggaaaaaa
      361 gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc actttagcct
      421 gctctgcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg gcattgcatt
      481 cctgctgcaa acgttcacta atccccaatt cctccagcct cgtgtcaccg ctaatcacga
      541 ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc
      601 tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac gcgtcacagc
      661 gctgctgttg gttaagttgg gtaagccgct cgttagcata gttgttaagc ggcgcatctt
      721 ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta ttcaaaaatt
      781 cgctccgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca actacggaaa
      841 ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat cgcgccaaat
      901 ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcgcc ggccatcatc
      961 ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat cgcccgttgc
     1021 tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa aatcagtttt
     1081 gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga gcaggcgtag
     1141 ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc accaattggc
     1201 cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg acaagtattc
     1261 atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc cagcacctca
     1321 gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac gtgccccgct
     1381 tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc
     1441 gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg ctccattccc
     1501 atcacagcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc ttcattggtc
     1561 accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat acctatttca
     1621 gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc ttcaggcgtt
     1681 tccggcccct catagcccag acgttcgtgt agcactcgac ggatctgatg gcgatcttta
     1741 tgaattgccg ggaccacaac atgagatggc ggatcttgat ccagctggag aatatattca
     1801 cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
     1861 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
     1921 tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa accagacgtg accgccgttt
     1981 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
     2041 cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc gaccattttt
     2101 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
     2161 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
     2221 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
     2281 ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa ttaaatactc
     2341 agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg atatttcggc
     2401 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa
     2461 cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca ctcccagctt
     2521 acgggccagg ctacaagatg ggtgatacac agctctccct tgcaaactgg cacctacatc
     2581 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
     2641 ttcggcacgt gatgcccatt caggttcatc cgccagatac gtcgggtaac tttttacggc
     2701 ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca gtgcggcgat
     2761 cagatttttc atccctggaa tcgcttcttt gatataaccg ctattgatcg caggctgacc
     2821 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     2881 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     2941 attcacaact aatactccat tacttcatgc ccatttatgc ccgatggttc ccctccttcc
     3001 gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa cataccaccc
     3061 ctcatttgtg tgacaaatga taatctttca tccaaacttc aaatgcaata actaatttta
     3121 tttctttcgg catcgctcac attttatggg gaaatcgacg ttcatttttg gcataagaaa
     3181 tccatttaaa aaggctacat cccttatacc tattcagagc tacttattca ggggttattg
     3241 agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc aagtaatagt
     3301 cggggtttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac tgagctagag
     3361 tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg
     3421 ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt tattcagggt
     3481 gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc gcggacaaac
     3541 gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac cacaacaggg
     3601 agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt taatacagcc
     3661 agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg agacatgtgg
     3721 gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg gcatttccag catttgctgt
     3781 attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc tgtattaacc
     3841 tgtgcaatcc actcacgcac cgcgagggta aagaatgtag cgcagatctg gctacacagt
     3901 gcatccactc ccggcatcgc cgatctggat tcctgttgta aaaacgggat cgcctccctc
     3961 agccagttat attccacgct gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac
     4021 gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc cgcagacaat acaggtcgat
     4081 tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac cacatttccc
     4141 gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc ttgcgttaac
     4201 gcatgccaac gaataaccga taattccccg gcaccatgtt gaagctgcca gtcacttcct
     4261 aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa acggctaagg
     4321 gcatccatat cagactcctg agtggttttt gattacaaac accaacaatc ttagtaaata
     4381 actaatctaa taccatcaaa tggttaatca tgatttttgt tttgtgattc tgattaagaa
     4441 aaagaaaata ccagacgata agacaaaata tcaagcgttg tgaagaaatg gtatttgctc
     4501 ttttgcgtct atagtcatga tgtcaaatga acgtgtttcg acaggaaatc atcatgaata
     4561 aatatcaggc agttattatt ggttttggca aggctggaaa aacattagcc gtcacgctgg
     4621 caaaagcagg ttggcgagtg gctctcatcg aacaatcaaa tgcaatgtat ggcgggacct
     4681 gtattaatat cggctgcatc ccaaccaaaa cattggttca tgacgcacag cagcacacag
     4741 attttgtccg tgccatacag cgtaaaaatg aagtggttaa ttttttacgt aataagaatt
     4801 ttcataatct tgcggatatg cccaatatcg acgtgatcga cggccaggcg gagtttatca
     4861 ataatcatag cctgcgtgtt catcggcctg agggaaatct ggaaattcat ggcgagaaaa
     4921 tttttattaa taccggtgca caaaccgtgg ttccgccaat tcctggaatt accaccacgc
     4981 ctggtgtata tgacagcacc ggattactta atctaaaaga attgcctggg catttaggta
     5041 ttttgggcgg cggatatatt ggcgttgagt tcgcctctat gttcgctaat tttggcagca
     5101 aggtaaccat tttagaagca gcttcgctgt ttttgcctcg ggaagatcgg gatattgctg
     5161 ataatatcgc gacgatttta cgcgatcagg gcgtcgatat tatcctcaat gcccatgtgg
     5221 agcgaatcag tcaccatgaa aatcaagtgc aagtgcatag cgagcacgcc caactggcgg
     5281 tggatgcact gttaatagct tccggtcgtc aaccggctac cgcttcgtta catccagaaa
     5341 atgccggtat cgcagtaaac gagcgcgggg caattgtcgt tgacaagcaa ttacatacca
     5401 ccgcagacaa tatttgggcg atgggagatg ttaccggcgg gctgcaattt acttatattt
     5461 cactggatga ttaccgcatt gtgcgtgatg agttgctggg agaaggcaga cgtagtactg
     5521 atgatcggaa aaatgtgcct tattccgtat tcatgacacc gccactgtcc agggttggta
     5581 tgacagaaga acaggccaga gagagtggtg ctgatattca ggtggtgaca ttgcctgtag
     5641 ctgcaattcc gcgtgccaga gtgatgaatg atactcgtgg ggtattaaaa gcgattgttg
     5701 ataataaaac ccaacgtata ttaggggcat cactgctgtg tgttgactcc cacgagatga
     5761 tcaatatagt gaaaatggtg atggatgccg ggctgcctta tagcatatta cgcgatcaga
     5821 tatttactca tccgtcgatg agcgaatcac tcaatgatct attttcatta gtcaaataaa
     5881 ctcaaaatca gacgccagaa caaatattct ggcgtctcag agaaaagaat cttattaatt
     5941 ctctgttact ctataacttc ttaatcactt cattgatggt attttatatg tttaaaaaat
     6001 ctgttttatt tgcaacacta ttatctggcg ttatggaatt ttccaccaat gcagatgata
     6061 aaataattct gaaacatatc agcgtctcgt cagtatcagc atcaccgaca gttctggagg
     6121 ataccattgc tgatatagcc agaaaatata atgcttcatc ctggaaagtc acatcgatgc
     6181 gaattgataa taattcaacc gcaacagcag tattgtataa ataaggatgt tcacaatgga
     6241 aaaatacctg cacctgttaa gtcggggaga taaaattggc ctgtcattga ttcgtctgag
     6301 tattgcgatt gtttttatgt ggattgggtt attaaagttt gtcccttacg aggcagacag
     6361 cattacacca ttcgtcgcaa acagtccact aatgtcgttc ttttatgaac acccggaaga
     6421 ctataaacag tatctgactc acgaaggcga atacaaacca gaagcaaggg catggcaatc
     6481 ggccaataat acctatggtt tttccaacgg tcttggcgtc gtggaggtga ttattgctct
     6541 gctggttttg gctaatcctg tcaatcgctg gttaggttta ttgggaggac tgatggcata
     6601 taccacgccg ctggtaacac tctcattttt aatcaccacc ccggaggcat gggtacccgc
     6661 attgggtgac gctcatcatg gtttccctta tttatccggt gctggtcgct tggtattgaa
     6721 agatactctg atgctggcag gtgctgtcat gataatggca gattcggcgc gggaaattct
     6781 taaacaacgc agtaatgaat ccagttcaac gttaaaaact gaatattgat aatccgaaca
     6841 ctgtctgttg ttcacttttt caggcgtcag gccccctttc tgatgatgaa gcctgacgcc
     6901 atattaccgg agatatgaca gagtgaagat caatatcact cccctgttaa aagattcttg
     6961 tcgtctcagg taaaatatat gattaatatc aatcatgtat ttttacagca agacaaccac
     7021 gaacaaattc aggattaaaa tgttttaccg actccccaac ataacccaaa tcaagcgaac
     7081 taatttgtgc catttctttc tctgtcagtg agaaatccca gatagcaaaa ttttcttcga
     7141 tacgctgttg atgtgtcgat ttcggaataa cgaccactcc ccgttgaata ttccaacgca
     7201 gaatgacttg agaaatcgat ttttgatggg catctgcaat actctgaagc agattatttt
     7261 caaagggttt atgtcgtcca ccacctaacg gagcccatgc ttcaggctgc acgttataat
     7321 acttcatggt tgccagcgcc tctggttggg caaaataggg atgcaactcg acctggttga
     7381 ccatcggttt aactcttacc gtttcacaaa agttcgccag aacatgagga tagaaattgg
     7441 aaaccccaat tgcttttaat ttgccctctt cataggcatc ttcgagtgca cgccacacac
     7501 tgaaataatc gcgcatagcc tggtgcaata aatagaggtc gaagtactct agtcccgatt
     7561 tttttaatga tgcttcaata cctgctgctg ccgtatcctg attcaacata tcctgccccc
     7621 acaatttaga cgttataaat aactcatctc gggtacataa accttcagat atagcttcac
     7681 gaacggcttc accgacggca tcttcattac cgtataccgc tgcagtgtcg atgagtcgat
     7741 aacccgtgcg gatagcgttc aacactgact gtttgcacac gtttttatcg gtaacctgaa
     7801 aaacaccaaa tcccatcatc ggcattttca gattgttact taatacggaa aattccactg
     7861 tcagtccctc cattaggcat gaacaatgag tctacgttaa aacataacct caaattagta
     7921 tgtggatttt gatatcactt atgcaaaaaa ttcattaata atgtaggact gaaacctctc
     7981 tattttcggg gacaacgaag cagacgctac cagtgctttt gccttcgacc ttgctgtttt
     8041 tgatacactt agggccttgg gcataacgaa aatgtgcgat atcacgaaga attaatggag
     8101 aatg
//
LOCUS       DAETQN010000171         8054 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.173, whole genome shotgun sequence.
ACCESSION   DAETQN010000171 DAETQN010000000
VERSION     DAETQN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.173"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            103..468
                     /locus_tag="K0W68_003938"
                     /pseudo
     CDS             103..468
                     /locus_tag="K0W68_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
     gene            complement(525..2903)
                     /gene="ppsA"
                     /locus_tag="K0W68_003939"
     CDS             complement(525..2903)
                     /gene="ppsA"
                     /locus_tag="K0W68_003939"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBI3075367.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3236..4069
                     /gene="ppsR"
                     /locus_tag="K0W68_003940"
     CDS             3236..4069
                     /gene="ppsR"
                     /locus_tag="K0W68_003940"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBI3075368.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4226..5272
                     /gene="aroH"
                     /locus_tag="K0W68_003941"
     CDS             4226..5272
                     /gene="aroH"
                     /locus_tag="K0W68_003941"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBI3075369.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5404..5595
                     /gene="hemP"
                     /locus_tag="K0W68_003942"
     CDS             5404..5595
                     /gene="hemP"
                     /locus_tag="K0W68_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBI3075370.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5599..7035)
                     /locus_tag="K0W68_003943"
     CDS             complement(5599..7035)
                     /locus_tag="K0W68_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBI3075371.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(7098..7811)
                     /gene="ydiV"
                     /locus_tag="K0W68_003944"
     CDS             complement(7098..7811)
                     /gene="ydiV"
                     /locus_tag="K0W68_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBI3075372.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2073 a         2113 c         1910 g         1958 t
ORIGIN      
        1 ccgtcagtag ctgaacagga gggacagctg atagaaacag aagccactgg agcacctcaa
       61 aaacaccatc atacactaaa tcagtaagtt ggcagcatca ccaaaattac cggacgcaaa
      121 aaagatatta ttgtccgcgg cggcgaaaat attagcagcc gtgaagtaga agatatttta
      181 ttgcagcatc ctaaaattca cgatgcctgt gtggttgcaa tgcccgatga acgtttaggt
      241 gaacgatcat gcgcttatgt cgtgctgaaa gcgccgcatc attcattatc gctggaagag
      301 gtggtggctt tttttagccg taaacgggtc gcaaaatata aatatcctga acatatcgtg
      361 gtaatcgaaa aactaccgcg tactgcctca ggtaaaatac aaaagttttt gttaagaaaa
      421 gatattatgc ggcggttaac tcaggatgtc tgtgaagaga ttgaataagt ttcatcttcg
      481 gggatcacat aaccccggcg actgaacgcc gccggggatt tattttattt cttcagttca
      541 gccaagctta accaggtttg caccacggtg tccgggttca gagacaggct atcgatcccc
      601 tcttccatca accatgcggc aaagtcttcg tggtcggacg gaccctgacc gcaaatcccg
      661 acatatttgc cctgtttctt cgcggcacgg atagccatcg acagcagtgc tttcaccgca
      721 tcgttgcgct catcgaacaa ttcagacacc acgccggagt cacggtccag accgagcgcc
      781 agctgcgtca tatcgtttga gccaattgag aagccgtcga aatattcgag gaactgctcg
      841 gccagcaagg cgttggacgg gatttcacac atcatgatga ttttcagccc gttctcacca
      901 cgcttcagcc cctgacgcgc cagttcctca accaccgctt tcgcctgatc tacggtacgc
      961 acgaacggga tcatgatctc aacgttggtc agccccatgt cgttgcgcac acgtttcact
     1021 gcttcgcact ccagcgcgaa gcagtcgcgg aagctgtcgg aaacatagcg tcccgcgcca
     1081 cggaagccga gcatcgggtt ctcttcatct ggctcgtaac gctcaccacc gaccaggttg
     1141 gcatattcgt tcgatttaaa atcagagaga cggacaatga cgcgcttcgg ataaaacgcg
     1201 gcacccagcg tcgcgatccc ttcagtcaga cgaccaacgt aaaattcacg cggagaatca
     1261 aaacctttca tcatctcgcg gatttcattt tgcaactgcg gttcctgatc gtcaaactca
     1321 agcagtgcgc gtgggtggac gccaatcata cggttgatga taaattccag acgcgcaagg
     1381 cccacgcctt cgttcggcag gcaggcgaag tcgaaagcac ggtccgggtt accgacgttc
     1441 atcatcactt tcaacggcag atccggcatc atttctacgc tggagctttt cacgctaaat
     1501 tccagcaact ccgcatagac gtaaccggta tcaccttcgg cacaagaaac agtgacgttc
     1561 tcaccgtctt tcatccgttc tgttgcatca ccacagccca ctaccgccgg aatgcccagt
     1621 tcacgagcga tgatcgccgc gtgacaggta cgaccgccac ggttggtgac gatggcagat
     1681 gctttcttca tgatcggttc ccagtccggg tcggtcatgt cagtgaccag cacgtcacca
     1741 ggttcgatgc ggttcatttc gctgatctca tggatgactt tcaccggacc cgcgccgatg
     1801 cgatgaccga tagcacggcc ttcggcgata atcttaccct gtgaatgcag cgtataacgc
     1861 tccatgacct gaccgcgtga gcgcacggtt tccggacgcg cctgcacaat gaacagtttg
     1921 ccagtgtggc catctttcgc ccactcaata tccatcgggc gaccgtagtg tttctcaatt
     1981 tgtacggcct gttttgccag ttcctgcact tcttcgttgg tcagcgagaa gatgtcacgc
     2041 tgttcctgcg gtacgtcttc gattttaacc tgcttgccgt gctcctgggt cggcgcgtaa
     2101 accatgcgga tttttttcga ccccatggtg cgacgcacga tagccgggcg attcgccgcc
     2161 agtgtcggtt tatggacgta aaactcatcc gggttaaccg caccctgcac gaccatttcc
     2221 ccaaggcccc atgcggaagt gataaacacc acctggtcaa agccggattc ggtatcaatg
     2281 gagaacatca cgccagaaga tgcgaggtcg gagcgcacca tccgctgaac accggcagag
     2341 agcgccacgc cacggtgatc gtaaccctgg tgcacacgat aagagatggc gcgatcgtta
     2401 aacagagaag caaatacatg cttcactgcc acgagaacgg cgtcaaaacc ctgaacgttg
     2461 aggaaggttt cctgctgacc ggcaaaagaa gcgtccggca tatcttctgc ggtggcggag
     2521 gagcgcaccg caaaagaggc gttttcgtca tcggcggaaa gctgtgcata ggcttcgcgg
     2581 atggcgtttt ccagctcagg ctggaaggga gtgtcgataa tccactggcg gatttgcgcg
     2641 cccgctttcg caagctgagt aacatcgtca atatccgttt tatccagcag ttcataaatg
     2701 cgctggttta cgccgctttg gtccagaaac tggttaaacg cgtcggcggt tgtggcgaaa
     2761 ccattcggaa cggaaacacc cattccggaa aggttagtaa tcatttcacc cagggaggca
     2821 tttttgcccc caaccctgtc tacatcattc atgccgagtt ggttatacca aagcaccagc
     2881 ggtgacgagc cattgttgga catcgaacaa tccttttgtg ataaatgaac ggtttgagaa
     2941 acacatttct gcgcatttat ctttgcatat ttaaccggat gaaaaaaacg gtgaatcgtt
     3001 caagcaaata tattttttta ctttttaaga ctgatcccag cgttgcgcaa atctgctctc
     3061 ttcgacaatt tccacataaa ccatcggtat aaacgaaacc ataaaaatga aatgctgttt
     3121 tcataaaaaa taaaattgaa ggttcatttt ataaaccaga cataacgtcc tacgcttctg
     3181 tgcgttttta atttatgctt tcatagaatt atgtctgcat cacgggaaga acaaaatgga
     3241 taatgctgtt gatcgccacg ttttttatat ttctgatggt acggcaataa ctgcggaggt
     3301 attaggacac gcagtaatgt cacaatttcc cgtcactatc agcagcatta cgctgccgtt
     3361 tgtcgaaaat gagagccgtg cacgggcagt gaaggatcag attgacgcaa tttatcacca
     3421 gacaggtgtg cgcccgctgg tcttctactc catcgtgttg ccggagattc gcgccatcat
     3481 cttgcaaagt gaaggctttt gccaggatat cgttcaggcg ctggttgccc cgctacaaca
     3541 agagatgaaa ctggatccaa cgccgattgc tcatcgtacc catggcctta accctaataa
     3601 tctcaataaa tatgatgcgc gcattgcggc gattgattac accctcgccc acgatgacgg
     3661 catttcgttg cgcaatctgg atcaggctca ggtgatcctg ctcggtgttt ctcgctgtgg
     3721 taaaaccccc accagtcttt atctggcaat gcaatttggt atccgcgcgg caaactaccc
     3781 ctttattgcc gacgatatgg ataatctggt gctacccgcg tcgctcaaac cgcttcagca
     3841 taaattgttc ggcctgacta tcgacccgga acgtctggcg gcgattcgcg aggaacgtcg
     3901 ggagaacagt cgctatgcct cgcttcgtca gtgcaggatg gaagtcgcgg aagtggaagc
     3961 gttgtaccgt aaaaatcaga tcccgtggat taacagtacc aattattcgg tagaagagat
     4021 tgccaccaag atcctcgata tcatgggcct tagtcgccga atgtactaga gaactagtgc
     4081 attagcttat ttttttgtta tgatgctaac cacccggcga ggtgtgacac acctcgcact
     4141 tgaaatcagc agcgattggt ttatcgtgat gcgcatcact tcccggcagt cctgccgtag
     4201 aagcaacaaa tttctgagac ttgtaatgaa cagaactgac gaactccgta ctgcgcgtat
     4261 tgagagcctg gtaacgcccg ccgaactcgc gctacggtat cccgtaacgc ctggcgccgc
     4321 cacccatgtc accgactccc gccgcagaat tgaaaaaata ctcaatggtg aagataagcg
     4381 actgttggtc attattggcc cctgctcgat ccacgatctc accgctgcaa tggagtacgc
     4441 cccccgtctg cagtcgctgc gcaaccagta ccagtcacgg ctggaaatcg taatgcgcac
     4501 ctattttgaa aaaccacgaa ctgttgtcgg ctggaaagga ctaatctccg atccagattt
     4561 aaacggcagc tatcgggtaa atcacggtct ggagctggcg cgcaaattac ttttacaggt
     4621 aaatgagctg ggcgtcccaa ccgcgactga gttcctcgat atggtgaccg gtcagtttat
     4681 tgctgattta atcagttggg gcgcgattgg cgcacgtact accgaaagtc agatccaccg
     4741 tgaaatggct tcggcactct cctgtccggt aggttttaaa aatggtaccg atggcaatac
     4801 gcggattgct gtggatgcta tccgcgcagc ccgcgccagc catatgttcc tctcgccaga
     4861 caaaaatggt cagatgacca tctatcagac cagcggcaac ccgtatggcc acattattat
     4921 gcgtggcggc aaaaaaccga attatcatgc cgatgatatc gccgcagcct gcgatacgct
     4981 gcacgagttt gatttacctg aacatctggt ggtggatttc agccacggta actgccagaa
     5041 gcagcaccgt cgccagttag aagtttgtga ggatatttgt cagcaaatcc gcaatggctc
     5101 tacggcgatt gctggaatta tggcggaaag tttcctgctc gaaggaacgc aaaaaatcgt
     5161 cggcagtcag ccgctcactt acggtcaatc cattaccgac ccgtgtctgg gctgggaaga
     5221 taccgaacgc ctggtcgaaa aactcgcctc tgcggtagat acccgcttct gaatgcgtgc
     5281 ccattcctga cggaatgggc atttctgcgc aacttgttgt cttctcaaca aattactgct
     5341 tgctctggcc tgccataata ttgataataa gaatcattgt tatatcaatt attattaatc
     5401 tttatgcgtt atacggatag cagaaaactc acgcctgaaa cggacgccaa tcacaaggcc
     5461 gcttccccgc agcccatccg gcgaattacc agccagacac tgttaggtcc ggatggcaaa
     5521 ctgattatcg atcatgacgg gcaagaatat ctgctccgta aaactcaggc tggcaagctg
     5581 ctgttaacta agtagccttt aactcgagca gctgacttcc agccgtttac cccagtcagg
     5641 tggacgactg acatagtcat catctctatc gctgaaagga tttcgcaacg ccccatgcag
     5701 gcggtgcaat tccgtcatat cacccttttc tgccgcttca atcgcccgtt gcgccaacca
     5761 gttgcgcaat accagagcgg ggttaacgct ttgcatcaac tgctgacgct cactatcgct
     5821 aacctcgtct tgttgcaaac gcccccgata acgggcaaac cagccatcaa atgccgcacg
     5881 atcaataaac tcatcacgta gcggtgacgc cgtgctgtgc tgctcggtca gactcagcat
     5941 gcggaatgtg cgggtataat cgctgcgctc tcgcgccatc agactgaata attcattcag
     6001 tagcgcgtta tcctcttttt gctccgtgat gaagcccagt ttctgccgca tccgttgtcc
     6061 ataatgcgtc aacaaaacct gctgatagct gtccagggcc tcattcaggg catctacggc
     6121 aacaaatggt gacaatgtct gcgccagacg ctgtaaattc cacaacgcga cagcaggttg
     6181 attatcaaaa ctataacgcc cttgatgatc cgagtgatta caaataaaac cgggttcgta
     6241 atcatcaaga aaaccaaacg gcccgtaatc aagcgtcagt cccagcagcg acatgttgtc
     6301 ggtattcatc accccatgtg caaagccgac cgtctgccat tgggcaatta acgacgcggt
     6361 acgtgcgaca acatcgctaa accagagacg gtatttgtcc tcatcatctt caagatgtga
     6421 ccagtaatga cgaatagcaa agtcagctaa ctgacgaacc ttttccgact cgcggcggta
     6481 gtaaaaatgt tcgaaatgac caaagcgcag atgacttggt gccacacgca tcagcatcgc
     6541 gcctggctcc gccgtttccc gatacactgg cgaatcgctg gtgacgatac ttaacgcgcg
     6601 ggtcgtcgga atgcccagat aatgcatcgc ctcactggcg agactttctc gtatcgtcga
     6661 acgtaaaacc gcccgtccat cacccattcg cgaataaggc gtcaggccag cacctttcag
     6721 atgccagtcc attgtagtgc catcagcaag cagttgttcg ccgagtaaaa tgccgcgccc
     6781 atcacccagt tggcccgccc agacgccgaa ctgatgacca ctgtaaacct gcgccagtgg
     6841 tgacatacca ggcagtaggg cttcgccgcc ccagacacct gcgccatttt taaacagcga
     6901 cgatggaata ctcagcgtgt tagccagttc ggtattatgc caaatcagcc gggcattatt
     6961 taaaggcgta ggggaaagtg ctgtataggt ttctggcaat tcatcgcgcc agcgggtaac
     7021 aaaagacagg gtcatagatc ctcctgtttg atagtgtaga cggttactct cgtcttaaac
     7081 accagcaaac agaagggtta tcgctgaacc aacgtcgtta tctgatttat gggaacggca
     7141 ggccacaggc atccctggag agcatggaaa tcgaaggggg taatctgtgc caatatttct
     7201 gccgtatcaa tgcctcccgc aatgatgcaa ttgcaacaag gggaaatttg cgcctggatc
     7261 gcacgaataa acggttcgaa ggagcggtgc gtgatttgtt gctgaataaa acttttatcc
     7321 agcatgacgc gggtgaaaag accatcaaaa accgctttca ttgtgctatt gcctgctccc
     7381 aaattaccta acacaagggg atacacttgc gataaggata aaagaccgcg attatctttt
     7441 ccttcattga gatgcgggta attctcatta atcaacagtt caataaacgg atattttaat
     7501 agttcgcctg cgtaattatc cctctctaat aataatgtag ccacttgtgg cgttaaatta
     7561 agccaggcaa agagtttatg ttgaataaaa aaatgttggc atgattttag taattccaac
     7621 tgctctgaaa ataattgcca atgttgctcc tcggtaagtt gcgctataac ccgactcgtc
     7681 gggatacgca ccgtgccatc ttcactggag aaatgggtaa ttaactcaac gccgacaagg
     7741 acttgctggt tatctcttat cggcaggaaa tagcaatcag aatgataaag attctccaaa
     7801 aaaatcttca tggtaaccgt ccctctcgaa ggatgttttt cagtatccgg ctaaaaggga
     7861 tgaggccata agacataaca attacagaag gagtaacttt catttgttcc atgttaacca
     7921 ctttttcagg ggtccttttt ttagattatc ctgattataa acgaataatc catttacgga
     7981 atttttgtct gcaaaatact actgtattca ggagtaaatg gacggcgatg tatcatcgcc
     8041 gtccattttt cgac
//
LOCUS       DAETQN010000172         7968 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.174, whole genome shotgun sequence.
ACCESSION   DAETQN010000172 DAETQN010000000
VERSION     DAETQN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.174"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..239
                     /locus_tag="K0W68_003945"
     CDS             <1..239
                     /locus_tag="K0W68_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HBI3075373.1"
                     /translation="LLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIVQLLLEHG
                     ASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            279..1142
                     /locus_tag="K0W68_003946"
     CDS             279..1142
                     /locus_tag="K0W68_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBI3075374.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            1132..2679
                     /gene="fdrA"
                     /locus_tag="K0W68_003947"
     CDS             1132..2679
                     /gene="fdrA"
                     /locus_tag="K0W68_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBI3075375.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            2679..4037
                     /locus_tag="K0W68_003948"
     CDS             2679..4037
                     /locus_tag="K0W68_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBI3075376.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAGPPITWEKMCGAMKGAVTGAL
                     VFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGVTSASMFMHIVKNKTYGNIAY
                     TNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDAMKIIGEIDLRLMLAQALHMG
                     DECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEFVASSDYFSGPTWMAMCKAAM
                     DAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPAQQVIGPMFAGYKPEDSGLDI
                     GDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSRQMREITLGENPNVTIPLLGF
                     MGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAGIVHPPFACFEKAILGWCERY
                     GV"
     gene            4180..5130
                     /locus_tag="K0W68_003949"
     CDS             4180..5130
                     /locus_tag="K0W68_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HBI3075377.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADMVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            5140..6522
                     /locus_tag="K0W68_003950"
     CDS             5140..6522
                     /locus_tag="K0W68_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBI3075378.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            6899..7948
                     /gene="yahK"
                     /locus_tag="K0W68_003951"
     CDS             6899..7948
                     /gene="yahK"
                     /locus_tag="K0W68_003951"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HBI3075379.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1919 a         2019 c         2308 g         1722 t
ORIGIN      
        1 agcttttggc gcatacggag attaacgtta accagaccaa ccatgtaggc tggacgccgc
       61 tactggaagc gattgtgctt aatgatggtg gtattaaaca gcaggcgatt gtgcagttat
      121 tgctggaaca cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccactggaac
      181 tggcgcggga acggggcttt gaagagattg cgcagttact gattgccgca ggtgcataaa
      241 ccgggaggct ggctatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg
      301 cggattttct ggcgcagcgg gggcaaggac aggttgaaca ggtctttgcc agagcggtaa
      361 atatcgcact cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg
      421 cgccaaacag ttgtcggttg gcactcactc actttgatga tctgttccgg catggtgata
      481 aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca tcttcatata gagatgagtc
      541 gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg
      601 cctggctaca gtggcacgac attattcatc agcacctggg ggaaaatgaa accctgttta
      661 attatcgcgg cgataatccg ttttatcagg cgttaaataa agaattacat attaaacgac
      721 gggcagttat tcagacagta aacgataagc aaaatatcgc cgcagcggtc gccagtatga
      781 ttgggttagg gattggcctt acgccatcag ccgatgatta tttaacaggt ctggcgctta
      841 ttttatttat tcccgggcat ccggcagaaa aatacaaaga ggaattttat cgcggtctgc
      901 tacgcggcag aaataacacc acgttattaa gtgccataac gctggaagcc gcattacaac
      961 aacgctgccg ggaaaatatt catcgcttta ttcacaacat tatttatggc gtccctggga
     1021 attcaactca ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc
     1081 tgtatggcat ggccgatggt tgtgcgctga gccaaaccta cggagggaat tatgtcagtt
     1141 aaaatagtca ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg
     1201 cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac
     1261 aaaggtgtgc tgaaaaattt aggactgctg acgccgacgc tggaacaggc gaaaaacggc
     1321 gacctgatga ttgtcatcaa tggtaaatcg ggtgcggaca acgagcagtt gctggtggag
     1381 attgaagaac tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacgccact
     1441 attgccagcg ccaaaaagca tatcccggaa agtaacctgg cggtgatttc ggtcaacggt
     1501 ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt
     1561 tccgataacg tctcagttga agatgaactg gcgctcaagc agttggccca cgaaaaaggg
     1621 ctgctgatga tggggccgga ctgcggcacg gcgattatta acggcgcggc gctctgtttt
     1681 ggtaacgccg tgcgtcgcgg caatatcggt attgttggcg catccggcac cggcagccag
     1741 gagctgagcg tccgcattca tgaatttggt ggcggtgttt cacaactgat tggcaccggc
     1801 gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc tcgacgccat cgggatgctg
     1861 gaaaacgatc cgcaaactga aattatcgca ctgatttcaa aacctcccgc gcctgcggtg
     1921 gcccgcaaag tgctggaacg cgcacgtgcc tgccgcaagc cggtggtggt ctgcttcctc
     1981 ggtcgcgttg aaacgccggt ggatgagcag gggctacagt ttgcccgcgg cagcaaagag
     2041 gcggcgctaa aagcggtgat gctctccggc gtgaaacagg aaaatctcga cctgcatacg
     2101 cttaaccagc cgttgattgc tgatgtgcgt gcgcgtctgc aaccgcagca gaaatacatt
     2161 cgtggcctgt tctgcggcgg cacgctgtgc gacgaaacca tgttcgcggt gatggaaaaa
     2221 catggcgatg tctacagcaa cattcagccc gatccggaat tccgcctgca agatatcaac
     2281 cgcagcatca aacacacctt cctcgacttt ggcgatgacg acttcacgaa cggcaagccg
     2341 catccaatga ttgaccccac caaccgcatc agtcgcttga tcgaagaggc gcgcgatcca
     2401 gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc
     2461 ggctccacca tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag
     2521 ttgatcattc tcgcctatgt gctgggtacc gatcttgata cgccatcgtt agaacaacaa
     2581 agccagatgc tgcttgatgc cggagtgatt ctggcgagca gcagcaccaa taccggattg
     2641 ctggcgcgtg aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc
     2701 aaccattgaa cgttattaac gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc
     2761 atgtagaagt gactcaactc gactggacgc cgccggggca gggcaatatg caggtggtgc
     2821 aggcgctgga gcgcattatc cagtcgcatc cggtgctgat tggttttgat caggcgatta
     2881 acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc cgggccgccg atcacctggg
     2941 aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg
     3001 cgaaagatct cgacgaggcg gctgaactgg cggcctccgg ggagatcacc ttctcgccgt
     3061 gccacgaaca cgactgtgtg ggatcgatgg cgggcgttac ctcggcctcg atgtttatgc
     3121 acatcgtgaa aaacaaaacc tacggcaaca tcgcttacac caatatgagc gagcagatgg
     3181 cgaagatttt gcgtatgggt gccaacgatc agagcgtgat cgaccgcctc aactggatgc
     3241 gcgatgtgca ggggccaatg ctgcgcgacg cgatgaaaat tatcggtgag atcgatctgc
     3301 gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg ccataaccgc aataacgccg
     3361 ggacgacgct gctgattcag gcactgacgc cggggatcat tcaggcaggc tattccgtcg
     3421 agcaacagcg cgaagtgttc gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt
     3481 ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg catcgaatac agcaccgtgg
     3541 tcaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc
     3601 aatggtttac cggcccggcg cagcaggtga tcggcccgat gtttgccggt tataagccgg
     3661 aagattcggg gctggatatc ggcgacagcg ccatcaccga aacctacggt attggtggat
     3721 ttgctatggc gaccgcgcct gcaatcgtcg cgctggtggg cggcacggtg gaagaggcta
     3781 tcgatttctc ccgtcagatg cgtgaaatca ctctcggtga aaaccccaac gtcaccattc
     3841 cgctgctcgg ttttatgggc gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg
     3901 gcattctgcc ggtgatcaac accgccatcg cccataaaga tgcgggcgtc ggcatgattg
     3961 gcgcgggcat tgtgcatcca ccattcgctt gcttcgagaa agccattctc ggctggtgcg
     4021 aacgttacgg cgtctgactt acatgtttat gccggatgcg gcgtgaacgc cttatccggc
     4081 ctacggatgg cccgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
     4141 ggtcataacc tcatcaccct ctgtagcgga cataacaaca tgaaagagct tgtggtcgtt
     4201 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
     4261 gaggcggtga aagccgtggc cgatatggtg ctggaaatgc tggcttccga ttacgacatt
     4321 gtgctgaccc acggtaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
     4381 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcaga tacgcagggc
     4441 ggcatcggct atctgatcca acaggcgctg aataaccgac tggcgcgtca cggcgagaaa
     4501 aaagccgtca ccgtggtaac tcaggtggaa gtggataaaa acgatccagg ttttgcccat
     4561 cccaccaagc ccattggtgc attcttcagt gaaagccagc gtgacaaact acaaaaggca
     4621 aaccctgact ggtgttttgt tgaagatgcc ggacggggct atcgccgcgt ggtcgcctcg
     4681 ccggaaccga aacgtattgt cgaagcaccc gccattaagg cgctgatcca acaaggtttt
     4741 gtcgtaattg gcgcgggcgg cggtggaatt ccggtagtgc gtactgaagc gggggattac
     4801 caaagcgtgg acgcggttat tgacaaagat ctctctaccg cgctgctggc ccgtgaaatt
     4861 cacgccgaca ttctggtgat caccaccgac gtcgaaaaag tctgtatcca ctttggtaaa
     4921 ccgcagcagc aggcgctgga tcgggtggat attgccacca tgactcgcta tatgcaggaa
     4981 gggcattttt ctcccggcag catgttgcca aaaatcatcg ccagcctgac gttcctggag
     5041 cagggcggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctgcgcggt
     5101 gaaacgggca cccatattat taaaacgtaa ggacgtagga tgaaagaaag caatagccgc
     5161 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctat
     5221 gtgccgctcg cccatgcggc ggtagctggc accctaaact gcgaagcgaa caacaccatg
     5281 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     5341 gaaaacggtg tggcggtgca aacccgcacg gcgcgccaga ccgtggagat tcaggacggc
     5401 aaaattgtcg ccctgcgcga gaacaagcag catccggacg ccacgctgcc gcactatgac
     5461 gctggcggta agttgatgct acccaccact cgcgacatgc atattcatct cgacaaaacc
     5521 ttctacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccactat ccaggacatg
     5581 atcaaactca agcagaaaat gctgccggag ttacaaccgt atacgcagga gcgggcggaa
     5641 aaactgatcg atctattgca gtcgaaaggc accaccattg cccgcagcca ctgcaatatc
     5701 gaaccggttt ccggcctaaa aaatctgcaa aatttgcagg cggtgctggc gcggcgtcag
     5761 gcgggcttcg agtgtgaaat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg
     5821 gaacccttaa tgcgcgaagc gatgcaggcg ggtgcgcatt acgtcggcgg gctggacccg
     5881 accagtgtcg atggcgcgat ggaaaaatcc ctcgacacca tgtttcagat tgcgctggac
     5941 tacgacaaag gtgtcgatat tcacctgcac gaaaccactc cgtcgggcgt ggcagccatc
     6001 aattatatgg ttgaaacggt agagaaaacg ccgcaactga aaggtaagct gaccatcagc
     6061 cacgcctttg cgctggctac gctcaacgaa caacaggtcg atgcgctggc gaatcggatg
     6121 gcggcgcagc aaatttctat cgcctcgacg gtgccgattg gcacgctgca tatgccgctc
     6181 aaacagttgc acgacaaagg cgtaaaagtg atgactggca ctgacagcgt tatcgaccac
     6241 tggtcgcctt atggtctggg cgacatgctg gaaaaagcca atctctacgc gcagctctat
     6301 attcgtccta acgaacagaa cctctcccgt tcgctgtttc tagccactgg cgatgtattg
     6361 ccgctgaatg aaaaaggcga gcgtgtatgg ccaaaagcgc aggatgatgc cagctttgtg
     6421 ctggtggacg cctcctgttc cgccgaggcg gtggcgcgta tctcgccgag aaccgcaacg
     6481 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct
     6541 gcaacggtga attgtaagag atttaagccc cagggagtgg ggcgacctca atatgatagt
     6601 gtcggcagaa atggacatta tctttgagga atatgccctt attgacgttg tgtagataac
     6661 tatttgtcta cgtgaaaaga tcatcagttt tgccgatttt agtcttttac ggatagcaaa
     6721 tatcacactt ataggcgctc gccttatggg gaacgttgct gctctgtttc attcaggttc
     6781 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
     6841 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
     6901 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
     6961 acggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
     7021 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtaccggg
     7081 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
     7141 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgagga
     7201 cgggctggaa aactactgtg atcacatgat cggcacctat aactcgccga cgccggacga
     7261 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
     7321 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
     7381 caccacgtac tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgggcgtggt
     7441 cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg gggcacatgt
     7501 ggtggcattt accacttctg aggcaaaacg cgaagcggaa aaagccctgg gggccgatga
     7561 agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt tcgatttcat
     7621 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgtga
     7681 tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
     7741 cctgatcatg aaacgccgtg cgatagccga ttctatgatt ggcggcattc cagaaactca
     7801 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
     7861 cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
     7921 tatcgataat cgcacactaa cagattgaaa aaattaataa atacgctg
//
LOCUS       DAETQN010000173         7944 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.175, whole genome shotgun sequence.
ACCESSION   DAETQN010000173 DAETQN010000000
VERSION     DAETQN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.175"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            111..443
                     /locus_tag="K0W68_003952"
     CDS             111..443
                     /locus_tag="K0W68_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBI3075380.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            590..1951
                     /gene="yegQ"
                     /locus_tag="K0W68_003953"
     CDS             590..1951
                     /gene="yegQ"
                     /locus_tag="K0W68_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBI3075381.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2281..2598)
                     /locus_tag="K0W68_003954"
     CDS             complement(2281..2598)
                     /locus_tag="K0W68_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075382.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            3004..3903
                     /gene="yegS"
                     /locus_tag="K0W68_003955"
     CDS             3004..3903
                     /gene="yegS"
                     /locus_tag="K0W68_003955"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBI3075383.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            complement(3985..4758)
                     /locus_tag="K0W68_003956"
     CDS             complement(3985..4758)
                     /locus_tag="K0W68_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI3075384.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="K0W68_003957"
     CDS             complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="K0W68_003957"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBI3075385.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5952..7307)
                     /locus_tag="K0W68_003958"
     CDS             complement(5952..7307)
                     /locus_tag="K0W68_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBI3075386.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="K0W68_003959"
     CDS             complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="K0W68_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBI3075387.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(7626..>7944)
                     /gene="gatA"
                     /locus_tag="K0W68_003960"
     CDS             complement(7626..>7944)
                     /gene="gatA"
                     /locus_tag="K0W68_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBI3075388.1"
                     /translation="EFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADD
                     DNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLD
                     SDE"
BASE COUNT         2148 a         2036 c         1783 g         1977 t
ORIGIN      
        1 tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac
       61 agcaccttta gatgctgttt tcgatacgca atttcaatca aggagtcatt atggctggtt
      121 ggtttgaact cagtaagagc agtgataatc agttccggtt tgtgctaaaa gcgggcaatg
      181 gtgagactat cctcaccagc gagctgtata catcaaaagc ctctgcggaa aagggcatcg
      241 cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagtaacg
      301 gcaaattcta tttcaatctg aaagccgcca atcatcaaat tatcggctcc agccagatgt
      361 acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc
      421 agacggtgaa agacaatacg taattacgat gccgggcgtg gggtgtcggc gtccggcgct
      481 ttaccagatc cccccacagc gctacaatgc ccgcccttaa agtgggggca ctcccctaac
      541 cgcttcatca ggtgaagcgg agctgacctg tcatcagaac gagagaatta tgtttaaacc
      601 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
      661 agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt gtgcgcaaca acgaattcaa
      721 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
      781 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
      841 ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt
      901 gcgtgagcac ttccctgaaa tgccgatcca cctctcggtg caggctaacg ccgtaaactg
      961 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgagct
     1021 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga ttgagatctt
     1081 cgttcacggt gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
     1141 caagcgcgat ccgaaccagg gtacctgcac caacgcctgc cgctgggagt acaacgtcca
     1201 ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga ttccggtgca
     1261 aaatgttgag ccgacgctag gtatcggcgc gccaaccgac aaagtgttta tgatcgaaga
     1321 agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
     1381 gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca
     1441 ttcgctgaaa atcgaaggcc gtactaaatc tttctactat tgcgcacgca ccgcacaggt
     1501 ttatcgtaaa gctatcgatg atgccgctgc gggcaaaccg ttcgatacca gcctgctgga
     1561 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
     1621 cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg
     1681 tgagtttacc ggtgagcgca agggggacct cgcagcggta gcggtgaaaa ataaattctc
     1741 cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaacttta cccttgagca
     1801 catggaaaac gccaaaggtg aagcgatgcc ggtcgcacca ggcgatggtt atactgtgtg
     1861 gctcccggtc ccgcaggatc ttgagctaaa ttacgcgctg ctgatgcgta atttctccgg
     1921 ggaaaccacg cgtaacccac acggtaagtg attaatttcg attatttttc ccggatggaa
     1981 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
     2041 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
     2101 cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt cttccattga
     2161 ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc atcgtgctcg
     2221 cacaggttcc ggctaagcac aaccagaacg cgcatgtttg atgcttacca aaaaatattc
     2281 tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac cttctgtctt
     2341 caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg catcattttt
     2401 tactatattt tctgcaccag atattcttcc cctagctaaa gaagcttcgg cttcggtgta
     2461 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
     2521 acttgtttca tcattatgca cagcacaacc agatataaga aaaatactaa ttaatgatat
     2581 agcagctatc tttttcatct caccttcccc cattaaatac caacgacact ctctagtgtt
     2641 taaatataat aatggcatga ttattataat tgaataggat tataataaat gttctgtaca
     2701 acatttccta cataagtagg aattacggac attgaggccc ttcagggtaa ctccatgggg
     2761 gctttaatat attatattga agatgccact gtttagttga atattaggta tatgctcttt
     2821 tttgaaattt atcggtggca gccgttagta ttcgctgtcc ccattgcaag ctcctggtgg
     2881 taaccactga atcctccata cttgaactga ctttttatcc tccgactttc atcctgttct
     2941 gactccacct tttgttttct gctctacact atctacagac caatcataaa ggcacatacg
     3001 atcatggcag aatttcccgc cagcttactg attcttaatg gcaaaagtac tgacaatcta
     3061 cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa tgacgatcca tgtgcgggtc
     3121 acctgggaga aaggcgatgc cgcacgatat gtagaggagg cccggaagtt gggcgtcgca
     3181 acggtgattg ccggtggtgg cgatggcacc attaatgaag tttctacggc gttgattcag
     3241 tgtgaggggg atggcatacc cgcgctggga attttgccat taggaaccgc caatgatttt
     3301 gccaccagtg tagggattcc tgaggcactg gataaggcgc tgaaactggc aattgccggt
     3361 aacgccattg cgatagatat ggcgcaggtc aacaaacaaa cctgttttat taatatggcg
     3421 acaggcggat ttgggacgcg tattaccaca gaaacgccgg aaaaattaaa agccgcgctg
     3481 ggtggcgtct cttacatcat tcatggctta atgcgcatgg atactctgca accggaccgt
     3541 tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg ccctggtcat tggtattggt
     3601 aacgggcgtc aggccggtgg cggtcagcaa ttgtgtccga acgcgttaat taacgatggc
     3661 ttgctgcaac tgcgcatttt taccggcgat gaaattcttc cggctctcgt atcaacctta
     3721 aaatctgacg aagataaccc gaatattatc gaaggcgctt cgtcgtggtt tgatatacaa
     3781 gccccacacg aaatcacttt taatcttgat ggcgaaccgt tgagcggaca aaactttcat
     3841 attgaaatac ttccggcggc gttgcgttgt cgattaccac cagattgtct attattgcgt
     3901 taatcaaact attctctctt gaaccaggga gaatccgaaa tgccggatgc ggtgtaaata
     3961 ccttatccgg catacagaac aatactacac cgtaattaat tttacccgtg tcttctgcaa
     4021 tgccagttta tccacctctg atacagcatc atcggtaata ataatgtcaa ttttttcgat
     4081 tggcagtacc tgattaaaac cgcgacggtt aaatttcgat gaatcgagaa cggcaatcac
     4141 tttattagcg gcggtgacca tcgcaccgct aatcgaataa ccttcattaa aggtcgtaat
     4201 accattaacc gcatcaatac catcagcacc gacaaacatt aaatcggcat taatatcctg
     4261 caatgaacgc tcggcaatgg aaccatgcat cgagcgcgtt ttatggcgga ccgtgccacc
     4321 acagacgacc agagtaatgt ctttattttc ggaaagggca aacgctgccg ggagactgtt
     4381 ggtaatcacc gtgatatttt tagcggtcat taatccttcg gcgataagca ttgtcgtact
     4441 tccgctgtcg agaataacgg tcatcccttc atggatcatt ttgactgccg cctgcgctat
     4501 tctgtttttt ggcgcgctgg cgagctgaaa tcgctcttta aacccgactt cctgggtctc
     4561 ggtttcacta ttaccggaca ttattttcgc cgcaccgcca tgaaagcgcg taaccacgcc
     4621 tttttgttcg agaaagcgca aatcggcacg gattgtcgct tccgaggcag caaatactcc
     4681 cgccagatcc tgaacaagca cggttccctg ttcattcact aattggatga tcttattcct
     4741 tcgctcgaat gaattcatcg tcatttaccc atattcaatt gtggctagtg taaacgaagg
     4801 gggaccattg gtgaataatc tgttttgcac aataccgggc cagcatcacg ctggcccgcg
     4861 gtttcatgga atgagcaaca ctttgcccgg catggcatta cgagcgatgt cacgcaccgc
     4921 ctgggtgaag ctttcaaagc ttccacggtg agcgattaat ggctccaggc ttaacttacg
     4981 ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc ggccaagggc tggaatagtt
     5041 catccaactg ccgataaccg tcagctcttt acgcaatatt ttgccaaacg ttgccgatgt
     5101 taaatgcaga tcctgatgca acgtgcccac cagcgccagt tgggcatgag gaccggcaat
     5161 ctctaccgcc agttcgacag tttgcggtac gccagccgtc tcgaggataa gctgattaaa
     5221 gcgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc atttcgctac tgttaaatgt
     5281 ttgcatcgca ccgaaagatt ttgccaatgc cagtttttct gagctaatgt cgatcgccgt
     5341 cacactcttt gctcccagcg cgacagcgca ctgaatagcc agcaggccaa tggttccggc
     5401 accaataata ataacgtttt tattctcaca accttgcgct aaatgaaaag catgcaggcc
     5461 aacggtaatc ggctcaataa aagccccatc ctcaataggc atatccgtgg gtagagcaaa
     5521 gacatttttt cgcttaacga caatatattc agcaaatcca ccatcacgcc gcgagccaat
     5581 aaaatcatat tttgcgcact gggaataaaa ccctttcaaa cactctggat aagtaaaaca
     5641 gggtaataac ggcacacagg caaccgcatc gccagggtgt aaatcatcaa caccggatcc
     5701 caccgcatca atatagccgc taaattcatg gcctaacgtt attggataat aatgtgcacc
     5761 atttttaaat atcctgggta aatcggaacc acataagccc gagctggcaa tttttacccg
     5821 cacctcatcc tgatgtttaa tttcaggaat gacgctttct gcaacgcgca cgataccatc
     5881 agtatcattc accattgatt tcataaaaac tcctgattgt taagggggat aacctccccc
     5941 tcaggtaaaa attattctgc gagaacggtt ttctcttgtt taataaaacc acgcgctcta
     6001 cgccaggtca tgaaaatacc ggtaagataa attgcgccga taataatgaa accgggaata
     6061 ttttgcgtgg agaaaacctg aatcagtaac caggtaatgg gagaaccgcc ctgatccatt
     6121 gaagccacca tacccccggc ttttaacgcc ccagcattag ccgccagttg ggtgtgcaaa
     6181 ccaatagttt gcgtcgcgat ccacagggtg atgctcataa tgatgacacc cgagattaag
     6241 gtgcggaaca gatttccacg atgcaccgcg accgccatcg ccacgaagaa gccgatggtg
     6301 gcaagatcgc caaacggcag tacctgatta cccggaacac aaacagcaat taaaatggtg
     6361 agcgggataa aaatcaggct tgccgatacc accgccgtat gccccagcag taatgcggga
     6421 tccaggccaa tcaggaactc ctgaccgccg aacttcgcct gtaaacgact acgggcctgc
     6481 ttagcgatgg gcgttaaacc atccatgatg ggtttaatca cccgtggcat cagcagcata
     6541 actgccgccg tttttaccgc cagctgcaat acacctttga catcgtaacc ggcgaggatg
     6601 ccgataatca gccccatcac aaaacccacg gtgacaggct cgccaaatgg accaaatttg
     6661 cgctgaatat cgtcggcgct aaatttaatt cggttaacgc ctgggatttt ctcgatgata
     6721 gcatcgacca gcaccgcaat cggccccata tacgccgacg taccgtgcgg aatagcaata
     6781 ccttccagct caaagaaatt tcgggtatcg cgggcaaacc agtcgccgag cttataaaca
     6841 aacgccgcgt gaattaccac acctgccatc cctatcatcc atgaaccggt tgccagatgc
     6901 agcaacgcgc cggtgaaggt catatgccag atattccaga tatcaacatt taccacccgc
     6961 gtcatacggg tcagtaacat cgccacgtta accagaatcg caatcggaat cgccaccagc
     7021 gcaatttgcg acgcccaggt cattggtgaa gagcccggcc agccgacatc gaccacatgc
     7081 agattcaggt cgaaattttc cgccatcgct ttcgccgccg gaccaatgga atccagcatt
     7141 aagccaatca caaggccaat gccaacaaag ccaatcccga tatgcagacc cgctttaaag
     7201 caatcgcctg ccttcatgcc taatatttta gaaaaaataa tgatgacaat cggaagcatc
     7261 accgtagggc cgaggtcgag aatataacgc atgacttctg aaaacatagg tcacccctgt
     7321 aagatagtca gaattttatt ttgtaatgct tcgataccga caccagaaat aaaaggcatg
     7381 ccgtgaacta acggaatatc gccaaaacta cgatccactt tggcagtggt gcatatcaaa
     7441 tgcacaccat ccatataggt ttctatttca ttaacccgac actggattaa ttcaacagga
     7501 atactatgat tctgacacaa ctctttaatt tcttccgccg ccatcgtaga ggtcgcaacc
     7561 gcgcctccgc aagcgacaat aatcttgcgt ttcataaata gtccttttta ttgttacaga
     7621 gggatttatt cgtctgaatc taaaacatac tttgtgaagt attcctttaa ctgggtttca
     7681 ggaagagtga ttagtgtctc gacgatatcg ggctgttgta acttgccaaa taaacagcgt
     7741 aaaagtttca attgctgctg cggattctcc acaatcaacg caataaccaa cgatacctcc
     7801 acgtcgttat catcatccgc ttgctgaaaa tgaactttat ttgttggcct taacagataa
     7861 atggctgacg acttagcatg aatcgcctca caatgcggta ttgcaatggc gtgctgctca
     7921 agcattatcc cggtagggaa ttct
//
LOCUS       DAETQN010000174         7936 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.176, whole genome shotgun sequence.
ACCESSION   DAETQN010000174 DAETQN010000000
VERSION     DAETQN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.176"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            16..513
                     /locus_tag="K0W68_003961"
     CDS             16..513
                     /locus_tag="K0W68_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBI3075389.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYILYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGYIKGACDQLCRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            510..980
                     /locus_tag="K0W68_003962"
     CDS             510..980
                     /locus_tag="K0W68_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBI3075390.1"
                     /translation="MSRVTAIISALVICIIVRLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(1069..1581)
                     /locus_tag="K0W68_003963"
     CDS             complement(1069..1581)
                     /locus_tag="K0W68_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HBI3075391.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSHSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1776..2540
                     /locus_tag="K0W68_003964"
     CDS             1776..2540
                     /locus_tag="K0W68_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075392.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            2491..3894
                     /locus_tag="K0W68_003965"
     CDS             2491..3894
                     /locus_tag="K0W68_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBI3075393.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3894..5360
                     /locus_tag="K0W68_003966"
                     /pseudo
     CDS             3894..5360
                     /locus_tag="K0W68_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            5251..5994
                     /locus_tag="K0W68_003967"
     CDS             5251..5994
                     /locus_tag="K0W68_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBI3075394.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5998..7218
                     /locus_tag="K0W68_003968"
     CDS             5998..7218
                     /locus_tag="K0W68_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBI3075395.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            7222..7728
                     /locus_tag="K0W68_003969"
     CDS             7222..7728
                     /locus_tag="K0W68_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075396.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
     gene            7799..>7936
                     /locus_tag="K0W68_003970"
     CDS             7799..>7936
                     /locus_tag="K0W68_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3075397.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YT"
BASE COUNT         2100 a         1893 c         2158 g         1785 t
ORIGIN      
        1 tgcgagaggt gaataatgtc gccatcatta cgcaaggctg ttgcagttgc tattggtggc
       61 ggggctgttg ctatagcatc tgtgttaatc actggcccag gtggtaacga tggtctggaa
      121 ggtgtcagct acatactata caaagatatc attggtgtat ggactgtatg tcacggacac
      181 accggaaaag acatcatgcc tggtaaaacg tataccgaag cagaatgcaa agctctcctg
      241 aataaagacc ttgccactgt cgccagacaa attaacccgt acatcaaagt cgatataccg
      301 gaaacaacgc gcggcgctct ttactcgttc gtttacaacg tgggcgctgg caatttcaga
      361 acatcgacgc ttcttcgcaa aataaaccag ggctatatca aaggcgcatg tgaccagcta
      421 tgtcgctgga catatgctgg cggtaagcaa tggaaagggc tgatgacccg tcgtgagatt
      481 gagcgtgaag tctgtttgtg ggggcagcaa tgagcagagt caccgcgatt atctccgctc
      541 tggttatctg catcatcgtc cgcctgtcat gggctgttaa tcattaccgt gataacgcca
      601 ttacctacaa agagcagcgc gacaaaaacg caagagaact gaagctggcg aacgcaacca
      661 ttactgacat gcagcagcgc cagcgtgatg ctgatgcact cgatgctaaa tacacgaagg
      721 agttagctga tgcgaaagct gaaaatgatg ctcttcggcg caagcttgat aatggtggtc
      781 gggtgctcgt caaaggaaaa tgccctgtgc catcctcagc cgaaacctcc agcgcctccg
      841 gcatgggcaa tgatgccacc gtcgaactct ctccagttgc tggacgaaac gttctcggta
      901 tccgggacgg aatcatcagc gaccagacag cattgagaat gcttcaggag tacatcagga
      961 tccaatgcct tggggggtag cggtaatttt actcattatc cttcaaatca gattctgttg
     1021 tcagaggaat gggggaagct ggattcgcaa gagcttttta tgggcagttt agctaaaaaa
     1081 aggcaacacg aagctaaaac taccaacata aagtgctgac cagctagcag ctgaaatcag
     1141 actcactgta tacactttca ctggatgaag ttgcaacatt cctgccacca aaggagcaat
     1201 ataacgcagc acggcgataa agcgcgaagt gaaaagtatt gacacagagt tattttgcaa
     1261 ctgaaggcga acccgttcta ttgtattgga atggcttgat attatacatg caagttgagg
     1321 gatatgccca attattatac caagatggta attaacgata gtaccgcacc atgcgccact
     1381 catgaccgtg attccagctt cccatggtga aagagtcgtc ttgctgactg taattacagt
     1441 ggccatcata attgatgctg gtggcaatat cgccgaaatg agtaaagtgg attttgtgaa
     1501 cgctattata aaaagaagcc cccataggcg atatggatga agtacaaaat agttcatcaa
     1561 agcatttatc cactccatca atgtctttcc ttctgtgaat ttttctttgc tatttaaccg
     1621 tgatacgact taatttaggc ttaaacacct tacatttaca aggtactcct gtggggggcc
     1681 ctgccacgga gcgtcgcagg agtgagtgat gaggaagaat taaagcaacc tattgtgaca
     1741 ggcagcgata acagtaaatc acaggaagca taatcatggc aaaaccggac tgggaggcca
     1801 tcgaatcggc ataccgggcc ggagtcctta gcctccgtga tataggcgag aaatacggcg
     1861 ttacagaagg ggctatcagg aagagggcca aaaagcttgg ttgggcacgc agtggcggta
     1921 cgcaggtttg caaaaatggt acgcaaaaaa ggaaagtgcg taccagcaga aagcctgcca
     1981 ttactggcct tacacaaaaa agtacgcaac taaaaacaga atctacaccg gatacgaaac
     2041 cgatacgcgg aatgcgtacc gatcccccga ctaacccatt ccaacccggt aaccagcagg
     2101 cattaaaaca cggtggttat gcccgtcgcc ttctgctcaa agatgaggtg atagaggacg
     2161 ctaaagcgtt gacgctcgag gacgaattat ttcgccttcg tgctaacaac cttgtcgctg
     2221 cagagaatat tggtcggtgg ttggtgtcgc tggaagatgc taatggggac caggaaagga
     2281 agatgctgat tgaaaatatc agcgccgccg agaaagcaat gatgcgcaat acagttcgta
     2341 ttgagtccat cgttggcacg cttgcgacgg taggaaaaat atttgctgat acagcctacc
     2401 gcaaggccgc cactgataag gtgtctctgg aggctgatcg tcttcgccgt gatgcaggta
     2461 ttgatgatgg caatggagag cgtgacctca atgacttcta ctctgacatc caaaccgacg
     2521 ctgaatccgg ctttacgtag tttctggact atgcgggcac gtaacaaagt gctttatggt
     2581 ggtcggtcat cgtcaaaatc atgggatgcc gctggcattg ccatatttct gtcgaataaa
     2641 tacaccctgc gtttttgttg tgcccgtcag atccagaata aaatcgaaga gtcggtgtat
     2701 accctgctca aaattcagat agacaggttt ggtctgcggc accgtttccg tattctgaac
     2761 aacaaaatca ttaaccgggt tactggctcg gaatttgttt tttatggatt atggcgcaac
     2821 atcgaagaaa ttaagtcact ggaggggatc gatgtgttgt ggctggaaga agcccacgca
     2881 ctgacggaat accagtggaa aattctggag ccaacgatcc gtaaagaggg ttcggaatgc
     2941 tggttcatat tcaaccccgg acttgttact gatttcgtct ggcgcaactt cgttgttgat
     3001 ccgcccgaag gcactctcat ccgcaaaatt aactatgacg aaaatccgtt tctgtctgac
     3061 accatgctta aggttatcga cgcggcgcga cgccgtgatc cggatggttt taaacatgtg
     3121 tatgagggcg ttccggagtc tgatgatgat gcggcaatca tcaaactgtc ttggatagaa
     3181 gccgcagtgg atgcgcacaa aacgttaaat ttcgaaccca gtggaagaaa gcgtattggc
     3241 tttgacgtgg ctgacagtgg tacagataag tgcgctaacg tttaccgtca cggatccgtt
     3301 gttttctggg ccgacgaatg gaaggccaaa gaagatgaat tactgaagag ctgccagcgt
     3361 acttatcagg cggcgctgga gcgtgaagca gatattgttt acgactctat cggtgttggt
     3421 gcgtctgccg gtgctaaatt ctctgaaatt aacgctgacc ggaagagcga gaacgcatac
     3481 gcgcgacgtg tgaattacca gaggtttaac gccggtgctg gtgtgcatga gccagatgac
     3541 gagtacaacg gcatccccaa caaagacttt ttcgcaaatc ttaaggctca ggcatggtgg
     3601 ctggtggctg accgtttcag aaatacgttt aacgccatta acaacggaga acagtatcct
     3661 gtggatgagc tgatcagcat agattctcgt tgtccgttgc ttgaaaagct gaaactggaa
     3721 ctgacaacac ctcatcgtga tttcgaccgt aacggacgtg tgatggtcga aagtaaaaaa
     3781 gacctcgcaa aacgcgagat accgtcacca aacgttgctg atgcattcat tatggccttc
     3841 gcgccaatcg atacatcgct ggatatctgg gaacagctgg ggagacaggc ctgatggcac
     3901 gaaacaaaca agccctgcgg cgaactgtgc aggccacagc tgatggttat gagaatttta
     3961 ttgcccgcgt agggatgcag acacctaacc agcactcagc atccacctac cgggctaatt
     4021 tcaccagtcg taaccgcatg ctggtggaat ggtcctatcg ttcatcctag atcatcggcg
     4081 aagcagtcga tgctatcccg gatgatatga cccgcaaagg cattcgcatc acttcggaaa
     4141 ttgatgcaaa agatcgtggc attctcgaat cacaactgga tgagttgcaa atctgggatg
     4201 cgctgaatga cgtgctgaaa tggtcgcgcc tctacggcgg cgcggtgggt ttcatcatga
     4261 ttgaggggta ggcaccaatg accccgctgc gacccgaaac catcggtaag ggcaagttta
     4321 aggggattct cccgctcgac cgctggatgg tcgacccggc actgacccgc cgcattaaag
     4381 atatggggcc ggacctgggt aaacctgagt tttacgatgt ggtgaccaca gcaacgggaa
     4441 ttcctgcctg gcgcattcat cacagtcgcc tgattcgctt tgatggcgtc acgttgccat
     4501 ttcagcagaa gatgaccgag aacgaatggg gaatgtcggt tgtagagcgt atctgggatc
     4561 gtcttaccgc gttcgacagc gctactgtcg gcgcggcgca gctggtctac aaggcgcatt
     4621 tgcgtaccta cagcgtggag aagctacgcg agcttatcgc acttggtggt cctgcgtatg
     4681 aagcgttgct gaagaatatc gacctgattc gacagttcca gagtaatgaa ggcatgacgc
     4741 tcatggactc gcgggataag tttgaaaccc atcagtacag cttcagtggt ctggatgaca
     4801 tcctttcgca gtttgtagaa cagattagtg gcgctgttgg tatcccactg gtgcggttgt
     4861 tcggacagtc cccgaaagga ttttctaccg gtgatgcaga ccttgccaac tattacgatc
     4921 gcatcagttc gttgcaggag aggcgtttac gtcttccggt gcggcggata ctggacatca
     4981 tgcatcgttc ggaacttggc aagccgctgc cggacgattt cacgtttgag tttaacccgc
     5041 tctggcaaat gtctgatgtc gatcgctcaa cggtggcgtt aaacaccacc aacgcaatca
     5101 gtacggcgct gggtgatggt ctgatgacac tgaaagccgc tatgactgat ttgcgagaaa
     5161 attctgacgt aaccggcatc ggggcatcca ttaccgacga ggacatcgag aatgccgaag
     5221 atgaagcgcc gcccggcatc ggcgaacctg atgacgaacc gcaggagccg tcaggcggaa
     5281 atccggtatc gaaccagcct acgcaggata gcgagggcgg tcggagacat cgtaaatggt
     5341 cgctacgatg gttcaaatga cagtatcacg gaaattattg cggcgctgga gcgttacagt
     5401 gaaatcatca ccccctgggc gacaaaggtc gcggaaaact ttactgcgga cctaacccgg
     5461 cagaacgaga aagtttggcg gcaacacagc aagaacatca gtcgcgagct ccgcaatctt
     5521 gtggaaagcg ctcctgtggg ccaggtgatg caatccatca tcgccgaaca ggtcaagtac
     5581 atcaaatctc tgcctctcga ggccgcagac agggtgtacg acatccagaa caaagcgata
     5641 gaggctgttg tcaccggtgg tcgggcggag cagtttgcta aagagattgc atctaccggc
     5701 gatgttgcta agtctagggc cgatctgatt gcccgaacgg aactgggaag agcaacgggc
     5761 gcgctggata tggcccgagc gatagctatt ggctcggatg gttatatttg gcgtacagcc
     5821 gatgatggcg atgtccgaga ttcccacgat cacatgaaag gtaaattcgt ccgctgggat
     5881 tcacctccaa ctttggacgg catgaccggc cacgcgggcg agctgccaaa ctgccgctgc
     5941 tataaagaga tcgtgtttgt tcgcgttcca ttcgcaatga aaagggcagc ataacccatg
     6001 aaatactttt ttgagaccag gctcggggaa acccgatacc gcctggctga cggctcgttg
     6061 ctgtgcaaag acgtgccgat aggacgaaca ggtaagcagc tctatggtgc tgatgacctg
     6121 ccaaaactga aacccgataa gttcggtgaa atagtcgtca cgcgttctcc tgagcaggta
     6181 ttccatccgg ccacgcttgc ctcattcgaa gggatgagca tcacgatcct gcatcctgaa
     6241 gatgaaaacg ggaatgtgcg gctggtaaat cccgagaact ggaaagagct tgctgtcggg
     6301 cacctccaga atgtccggcg cgggacgggt gagcagtctg atttgatgct ggctgacctt
     6361 atcgtcaaag acgaaagcgc cattcagctt atcgaagatg gcctgcgcga agtgtcgtgc
     6421 ggctatgacg cggagtacga gcagaccgag ccaggtaaag ccgagcaggt cgatattacc
     6481 ggaaaccatg tggctcttgt ccctaaaggc agagccggaa atcgttgtgc aattggagac
     6541 agagacacaa tggcaaatca aaagaaaaac tggtggaacc gcatgcgtgc ggccatcaag
     6601 acaggagatg ccgacaccat gaacgaactg gtggagtcgg ctcccgcatc ggttacagga
     6661 gatgaggggg atttgccgca gggcgttaat ctcaacatca acctgtcccc gcagcaacca
     6721 ctaccggaca aagcaccaga gatgggcgga gatccaaccg gcgacagtga tgatgacctc
     6781 aaaacattac tgaaagccct gctggctaag ctggaaagaa atgccacggg cgataacgat
     6841 aataagcctg acgataatcc gaccggtgac ggcgaggacg atgaagagga aaccacgatt
     6901 actggtgact cagcctggcg tgccgaagtt atcgttccgg gtatcgatct gagccgtaag
     6961 atgaaaccga ccgcgttcaa acgcgaggtt ctggcttctg ctgacaaaac gctggttcgc
     7021 cagatagtcg gtgatgcgga tatccgcaaa ttaccgaaac aatcggtcga catggcgttt
     7081 aatgccgtgt ctgaggttgc caaagggcga aacacccgcg ccaccaccgg cgatgcacag
     7141 cgcctaaaca tgggcatgac cagtatcgct tccctgaaca aacaaaacgc tgaattctgg
     7201 gcaaaccgta aagggtaaaa aatgaataat gtatttctgt accggatgcc tgttggcatt
     7261 gccggggctg tctctcgccc gcaggactta accgtcgaac cggtggtcct taaatccgat
     7321 aacgccttcg ctgcctatgg cctggctggt aaatacgatg atgacggttt tttcgtgccg
     7381 ctggcagatg gtgataccgc agacaaggtg aaggggatct acgtgcgccc ttatccgacc
     7441 acgtcgcagc cggacatggt tcgccaggtg ggaacaggca agaacttccc gggcgacgcc
     7501 atgaagcgtg gctacgtgac cgttaatctc ggttctgatt ttgatgccag cactatcaaa
     7561 aaaggcgatc cggtatacgt tgtcgtctcc actgatgaat ccatcaaagt gccgctgggt
     7621 ggattcatgt ccacgtcagt cagtggcaaa aacgtggtgc tgaccaacgc tgaattcaca
     7681 ggtgccggtg atgctgacgg caatgcagaa atttcctgga agatttaagg aacagacgaa
     7741 tgaggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt
     7801 ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg
     7861 caaaagcact gccggacatc agcgctatct ctgctctcac tgccgtaaaa catggcaact
     7921 gcagttcact tacacc
//
LOCUS       DAETQN010000175         7776 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.177, whole genome shotgun sequence.
ACCESSION   DAETQN010000175 DAETQN010000000
VERSION     DAETQN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.177"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1780)
                     /locus_tag="K0W68_003971"
     CDS             complement(<1..1780)
                     /locus_tag="K0W68_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBI3075398.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPEWLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="K0W68_003972"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="K0W68_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBI3075399.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="K0W68_003973"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="K0W68_003973"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBI3075400.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="K0W68_003974"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="K0W68_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBI3075401.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="K0W68_003975"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="K0W68_003975"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBI3075402.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="K0W68_003976"
     CDS             complement(5968..7005)
                     /locus_tag="K0W68_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBI3075403.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7338..>7776
                     /gene="yhhY"
                     /locus_tag="K0W68_003977"
     CDS             7338..>7776
                     /gene="yhhY"
                     /locus_tag="K0W68_003977"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI3075404.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1977 a         2101 c         1871 g         1827 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc attcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ttgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt atatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaag ccagcacccg
     7141 gctggctaag taatactgga agcaatgtga gcaatgtcgt gctttcaggt tctccgcgag
     7201 ggtcttcctg atcgcgagac aataataatc attctcattc gcacttgtcc aacacttttt
     7261 gcaaaaaaaa tgcatttgac tcgcatttga aagtcaatga tgttgaaagg gacatttacc
     7321 ccaaagagga caaaggaatg agtgagatag taatacgcca cgcagaaaca cgggattacg
     7381 aggccatcag gcagattcac gcccagccgg aggtgtattg caacacacta caggtgcctc
     7441 atccttccga tcatatgtgg caggagcgac tcgccgatcg tcccggcatc aagcaactcg
     7501 tcgcctgtat tgatggagac gtcgtgggcc atctcaccat tgacgtgcaa cagcacccac
     7561 gccgcagtca tgttgccgat tttggtatct gtgtcgactc tcgttggaag aaccgcggcg
     7621 tcgccagcgc cctgatgcga gagatgattg aaatgtgcga caactggttg cgggtagatc
     7681 gcattgaact aaccgtgttt gtcgataacg ccccggcaat taaggtctat aaaaaatacg
     7741 gctttgaaat tgaagggact ggtaagaagt acgcat
//
LOCUS       DAETQN010000176         7730 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.178, whole genome shotgun sequence.
ACCESSION   DAETQN010000176 DAETQN010000000
VERSION     DAETQN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.178"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            392..1078
                     /locus_tag="K0W68_003978"
                     /pseudo
     CDS             392..1078
                     /locus_tag="K0W68_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1411..2286
                     /locus_tag="K0W68_003979"
     CDS             1411..2286
                     /locus_tag="K0W68_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HBI3075405.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            2294..3070
                     /locus_tag="K0W68_003980"
     CDS             2294..3070
                     /locus_tag="K0W68_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HBI3075406.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            3239..5500
                     /locus_tag="K0W68_003981"
     CDS             3239..5500
                     /locus_tag="K0W68_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBI3075407.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYHSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            5569..6744
                     /gene="senB"
                     /locus_tag="K0W68_003982"
     CDS             5569..6744
                     /gene="senB"
                     /locus_tag="K0W68_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HBI3075408.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(7270..>7730)
                     /locus_tag="K0W68_003983"
     CDS             complement(7270..>7730)
                     /locus_tag="K0W68_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBI3075409.1"
                     /translation="QNGLAKALREIGRIERTLFMLDWFRDPALRRRVQAGLNKGEARN
                     ALARAVFMHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMERAVDALKRKGMKI
                     NAQLLSHLSPLGWEHINLTGDYIWKNSRIPTSGKFRRLRSVKIDKVKKQP"
BASE COUNT         2155 a         1794 c         1816 g         1965 t
ORIGIN      
        1 aaaaactggt cgcagaaaag tcctgttttc agcttggaaa cccggtaatc agaataatgg
       61 ataggggaac aaaaatctct gtatagaacg ctccattgag ggagagaaaa cgtgtcattt
      121 tgacccgttc ggtattcaga acacacaaaa ccgatcgcgg gtcgctttcg tgcatagtta
      181 tgcaacgcgc taaaaacgat tctgacgcat tttttatgtt taccctgttt tttcttgtct
      241 ttttgagttt tttgcgtcag agagcgtctg agagggcacg tacaacggga gttatggtaa
      301 atggatcggg ttttcgggaa ggttcgacag gatttgccgt tgggtgtagt gtaagcgact
      361 gaaaaacaaa cgcccctgaa atcatgtcca gatccggcag aaccagcctc atgcgcagtt
      421 ctggctggtt tttgacgttg atcgcgaggg ggcagcgatt gactggagcg accggaacgc
      481 tcccgcgccg aacatcaccg taaaaaatcc tgtgaacggt catgctcacc tgctctacgc
      541 gctcaatatc gccgtgagaa ccgcgccgga cagctcggtt aaggcgctga aatacgccgc
      601 tgcgattgaa cgttcgctgt gtgagaaact ttgtgctgat gtgaactaca gcggactgat
      661 ttgtaaaaat ccgttccacc tggaatggca ggtgatggag tggcgagagg aagcctatac
      721 cctcgatgaa ctggctgatt atctcgattt gagcgcctca gcgcgccgta gcatcgataa
      781 acattacggg atggggcgaa actgccacct gttcgaaatg acgcgcaaat gggcttacag
      841 ggcgattcgt cagggctggc ccgaattctc acagtggctt gatgccgtga ttcagcgtgt
      901 cgaaatgtac aacgcatcgc ttcccgttcc gctttcaccg cctgaatgtc gggctattgg
      961 caagagcatt gcgaaataca cgcacaggaa cttcacgccg gaaactttcg cacagtatgt
     1021 ggctgatacg cacacattaa cgtacgtttt cgttccattg gccctcaccc cccgataacg
     1081 acccaggtag cgcgccgtat tccccccgcc ggccgttttc ttcgacatgt acatcaggcg
     1141 cggaactcat cggggcgcgg gaatgaaatc ccattcagac tccggataga ttcaagcaag
     1201 cccacttatc gtcaaaatcg gtgttgcaaa aacgggagtg accatagatc cgttttccaa
     1261 gcggacccca taatatcggc tggtaaacat cactcgctag tgcattcttt ttgttggtaa
     1321 tgataatcat ttacattcat tctttgatgt tttatcataa ttaatgtttt gtcaataaat
     1381 atatggaaac atattgtttc agatgggaat atgagaaagt tcatccttat ttctatgata
     1441 acgttagcta gtgttagtct gggagcctgc agacagaatg tcaccataaa gcaggatgcg
     1501 ccagggcaga aagctttcct gactgacggg cagatttacg atttgcacag tggcaaaatt
     1561 atttctagca gcgagctttt ggcggatctt gctaccgctc gacatcttat tattggtgaa
     1621 aaacacgata atgccgagca tcatcagatt gagctttggc ttattcaaaa tttgctcatc
     1681 cagcgaccac aggggagtgt gttgcttgag atgttgacgt cagagcaaca gccccgagtt
     1741 aaccaagtta aatgttggct gaaagataat cctgtggttc gagatagccg tgttcaggaa
     1801 ctacttaact ggcaaaaagg gtggtcctgg gagatgtatg gcgacatcgt catgcagcta
     1861 ttacgtgggc cttacccgct gctcaatgcc aacattggcc gggagcagat gttggcactg
     1921 tacaaaaaaa acgaatttcc taaaggaaaa aaatccactg cacctgtggt acaagaagct
     1981 ctgcgtgaaa ccatcatatc tatgcatgaa ggcaaccttg aaagccagca actgacgtca
     2041 atgctctcta ttcagcagca gcgggatcgc tacatggccc gccagcttct gagtgccccg
     2101 gtaccgtcgt tgttgattgc aggtggttat cacgcgtcaa aaagcatggg cgtaccgtta
     2161 cacatggaag atcttgccac cggcacccat ccagtggtgt tgatgcttgc agaaaaagga
     2221 atgaacatca cagtagatca tgctgactac gtgtggtttg tagcgccaga tacaacaaaa
     2281 aggtaaacca tggatgatga atattctcca cttctcacag tcggtaaaat ggagcagctg
     2341 gtttatctgt tctctactgc ttcatggact gattttcctt gcgtttatct ggcgtttttc
     2401 cgaagttcag cccgctatgt ctcctgcacc ggcgatcatg ctgcaatggg cagaaattat
     2461 agaggctcct tcttctccac tttcgcttcc ggtgggaatc gctcaacagg agtccgcagt
     2521 gacagaagaa aagcaacaaa ctgaggacag acaacaacgt cctgtcactg aagacagtga
     2581 tgcaaccatt gaaataacta ggaagaaaaa gtcatccgat ggagaaaaga aaaagacacg
     2641 tccacctcgt aagataaagg ctcaaaccag cgacagcaac cccacagccg tttccagtaa
     2701 tgctgcacct caggctctgg tcgaatcgtc ccgtattgct gcgcctttta acagtgactc
     2761 aacaaagcga gataattctg aggcatcttg ggaaagcaga gtaaaaggcc acctaaaccg
     2821 ttataaacgt taccctggcg atgccagaaa acgtgcacga accggcacag ccgtcgtgac
     2881 ttttaccgta aacacggaag gcactattgt cagtagtttc ttggaaatct cttcaggaac
     2941 cctttctctt gaccgcgaag ctattgctgt tttagaacgc gctcaaccac ttcccaaacc
     3001 accgcctgaa atattggagg gaggattatt taaagtaaaa atgccgatta cctttaagtt
     3061 gaaggagtaa agggataatt ggccgttcaa gttcatttaa ctgaaattta attttcattt
     3121 ttatgctgaa cattttatca aacttttcca ctgtcgaaaa aatcaaaaag acagtagaac
     3181 ataattaata aacaaatagt tatacttatc tttattaata ttatacccaa ggaaatttat
     3241 gaacgttata aaactggcta tcggctcagg catattattg ctcagctgcg gtgcttactc
     3301 acaatccatc agtgaaaaaa ctaattccga caaaaaagga gcggcagaat tcagtccgct
     3361 cagcgtttct gtcgggaaga cgaccagtga gcaggaagct ctcgagaaaa caggcgcgac
     3421 cagttcccgg acaacggaca aaaacctgca atcacttgac gcaacagtgc gtagtatgcc
     3481 tggtacttat actcaaatag atcctggtca gggagcaatc agtgtgaata ttcgaggcat
     3541 gagcggattt ggtcgtgtaa acactatggt cgatggtatt acccagagtt tttacggaac
     3601 ctctacctcc ggaacaacga cgcatggttc aactaacaat atggctggcg tacttataga
     3661 tcctaactta ctggtagcag ttgatgttac acgcggtgac agcagtggct ctgaagggat
     3721 caacgccctt gccggtagtg caaatatgcg tactattggc gttgacgatg taatatttaa
     3781 cggtaataca tatggccttc gttcacgttt ctctgtcggt agtaatgggc tgggacgcag
     3841 cggaatgatc gcccttggtg gaaaaagcga cgcttttacg gatacgggaa gcattggcgt
     3901 tatggctgct gtgagcggca gttctgtgta ctctaatttc tcaaatggtt ctggaattaa
     3961 cagcaaagag tttggttatg ataaatatat gaagcagaac cccaaatccc aactgtataa
     4021 aatggatatc agaccagacg aatttaacag cttcgaactt tccgctcgaa cctatgaaaa
     4081 taaatttaca cgtcgtgata taaccagtga cgactattac attaaatatc attacacccc
     4141 tttttctgaa ttaattgact ttaacgtaac ggccagtacc agtcgcggta atcaaaagta
     4201 tcgtgatggc tcgctgtata ctttctacaa aacctcagcg caaaatcgtt ctgacgcgct
     4261 ggatatcaac aataccagcc ggttcactgt cgcggacaat gacctggagt ttatgctggg
     4321 cagcaaactg atgcgtaccc gctatgaccg gaccattcac tcagcggcgg gcgacccgaa
     4381 agcgaatcag gaatcgatcg agaacaatcc gttcgcaccc tccggccagc aggatatttc
     4441 agcgctgtat accgggctga aggttacgcg cggcatctgg gaggcagatt tcaatctcaa
     4501 ctacacacgt aacaggatca cagggtacaa gcccgcctgc gattcacgcg ttatctgcgt
     4561 gccacagggt agctacgata ttgacgataa agagggtggc ttcaaccctt cagttcagct
     4621 ttctgctcag gtaacaccat ggcttcagcc gttcattggc tacagcaaat ccatgcgcgc
     4681 cccgaacatc caggagatgt tcttctctaa ttcaggaggc gcatccatga acccattcct
     4741 gaagcctgaa cgtgcagaaa cctggcaggc gggttttaac attgatacca gagatttact
     4801 ggtcgaacag gatgccctgc gctttaaggc tctggcgtac cacagcagga tccagaacta
     4861 catctacagc gagtcttatc tggtttgttc tggaggtcgt aaatgcagta tgcctgaggt
     4921 gattggcaat gactgggagg gcattagcga tgaatacagc gacaatatgt acatctacgt
     4981 taactcggca agcgacgtta tcgcaaaggg cttcgaactg gagatggatt atgatgcagg
     5041 ttttgctttt ggccgactct ctttcagcca gcagcaaaca gaccagccaa cctccatcgc
     5101 cagcacctac tttggcgcag gggatatgac cgaactgccc agaaaataca tgacgctgga
     5161 tactggtgtt cgcttcttcg ataacgcgtt gaccctgggc actatcataa aatacacagg
     5221 caaggctcgt cgcctgtcgc ctgattttga gcaggacgaa cataccggcg caataatcaa
     5281 acaggatttg ccgcagatcc caacgattat cgatctctat ggtacttacg agtacaaccg
     5341 caacctaaca ctgaaacttt cggtacaaaa cctgatgaac agagattatt cggaggcgct
     5401 gaataagctc aacatgatgc caggtcttgg tgacgagacc cacccagcca attccgcgcg
     5461 tggcagaaca tggatatttg gcggggacat tcgtttctga tgacaatttc cgctaggttc
     5521 agacaatacg ttttctctct tatgtcaatt ttattgcagg aacgaaaaat gaatattttc
     5581 actttatcca aagcaccgct atacctgtta atttcactat ttttacccac gatggccatg
     5641 gctatcgatc cacctgaacg cgaactttcg cgatttgccc tgaaaacgaa ttaccttcag
     5701 tcccctgatg aaggcgtcta tgaactggcg tttgataatg ccagtaaaaa ggtgtttgca
     5761 gcagtcaccg atcgtgtaaa tcgtgaagcc aataaaggct atctgtattc gtttaattca
     5821 gattcgctga aagtcgaaaa taaatacacg atgccatacc gggcattttc gctggcgata
     5881 aatcaggata aacatcagct ctatatcgga cacacccagt cagcgtccct gcgtatcagt
     5941 atgtttgaca ccccaaccgg caaactggta agaaccagcg acaggttaag ttttaaagcg
     6001 gcaaacgctg cagattcgcg ttttgagcat tttcgccata tggtttacag ccaggattcc
     6061 gataccctgt ttgtgagtta tagcaatatg ctgaaaacgg ccgagggcat gaagcctctg
     6121 cataagctgt taatgctcga cgggacgacg cttgccttaa aaggcgaggt taaggatgct
     6181 tacaaaggta cagcgtatgg tctgacgatg gatgaaaaaa cacagaaaat ctacgttggc
     6241 ggaagagatt acatcaacga aattgatgcg aaaaatcaga cgctgctgcg taccatcccg
     6301 ttgaaagatc cgagaccaca aatcacaagt gtgcagaatc tggcggtgga ctccgcttct
     6361 gaccgtgcct ttgtggtggt attcgaccat gacgatcgtt ccggtacaaa agatggactc
     6421 tatatttttg acttacgcga cggtaaacag cttggctatg tgcacacagg agccggagct
     6481 aacgcggtga aatacaatcc gaaatataac gaactgtatg tcaccaactt cactagcggc
     6541 accatcagcg tagtggatgc caccaaatac agcatcaccc gtgaatttaa catgccggtc
     6601 tacccaaacc agatggtgtt gtcggacgat atggataccc tttacattgg catcaaagaa
     6661 ggctttaacc gcgattggga tcctgatgtg tttgtggaag gagctaaaga acgtattctg
     6721 agcattgatt tgaaaaagtc gtgatcgggg ggaaaaaaca tgggataaaa actccgttat
     6781 tccactaaac tggccggact aggaaaagca aacatctctg tcctgccggt acgcctgtta
     6841 cgccgctcca tgaggctgag gaatctccag aaaacgagac aggcacagca ttgtacgatc
     6901 aactgatttg tgccaataat taaatgaaat cacagctatg cctgttcatg gggttctagg
     6961 ccggaacccc agatttttcc gattgtttgt tttctaactg attattcagt agttttagag
     7021 ggctgacgct cagtggaacg aaaactcacg ttaagaggtt ttgactatga taaaagtcaa
     7081 agtaggggtg agtttggata tggaaattat tgtactataa ggagagcatg aaaacccctg
     7141 gggcagtcac tgaatgaggg accagcagcg gtaaaataac cagcataatt tctgcatggt
     7201 cgatttttat accgtgacag gctgtttttt atacttgggg tcagtttgga tatcgaaaat
     7261 tattgtacgt taaggttgtt ttttgacctt gtcgatctta actgatctca aacgacgaaa
     7321 cttacctgaa gtcggtatcc ggctgttttt ccagatataa tcgcctgtca gattgatatg
     7381 ttcccaacct aatggtgata aatgcgataa tagttgagca tttattttca tgcccttacg
     7441 cttcagagca tcaaccgctc tttccatgta aactgtgttc cataaggaaa tagccgccgt
     7501 caataaagtc aatccactgg cgcgataact ttgattttcc ggtttccgat ctctaatctc
     7561 ccccaatcga tgcatgaata cagcacgggc cagagcatta cgggcttctc ctttgttcag
     7621 cccggcctgg acacgtcgcc gcaatgccgg atcccgaaac cagtccagca taaacagtgt
     7681 tcgttctata cggccaatct ctctcagagc tttagccaac ccattttgtt
//
LOCUS       DAETQN010000177         7715 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.179, whole genome shotgun sequence.
ACCESSION   DAETQN010000177 DAETQN010000000
VERSION     DAETQN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.179"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..643
                     /locus_tag="K0W68_003984"
     CDS             <1..643
                     /locus_tag="K0W68_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3075410.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="K0W68_003985"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="K0W68_003985"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HBI3075411.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="K0W68_003986"
     CDS             2474..2986
                     /locus_tag="K0W68_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBI3075412.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="K0W68_003987"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="K0W68_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBI3075413.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="K0W68_003988"
     CDS             5579..5782
                     /locus_tag="K0W68_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBI3075414.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="K0W68_003989"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="K0W68_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBI3075415.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7715)
                     /locus_tag="K0W68_003990"
     CDS             complement(6827..>7715)
                     /locus_tag="K0W68_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HBI3075416.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1785 a         2101 c         2020 g         1809 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt ttgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaaaaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccacactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg gacgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcga
//
LOCUS       DAETQN010000178         7623 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.180, whole genome shotgun sequence.
ACCESSION   DAETQN010000178 DAETQN010000000
VERSION     DAETQN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.180"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..96
                     /locus_tag="K0W68_003991"
     CDS             <1..96
                     /locus_tag="K0W68_003991"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI3075417.1"
                     /translation="PAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(152..487)
                     /locus_tag="K0W68_003992"
     CDS             complement(152..487)
                     /locus_tag="K0W68_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBI3075418.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="K0W68_003993"
     CDS             complement(491..835)
                     /locus_tag="K0W68_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBI3075419.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /locus_tag="K0W68_003994"
     CDS             complement(837..1010)
                     /locus_tag="K0W68_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075420.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /locus_tag="K0W68_003995"
     CDS             1111..1296
                     /locus_tag="K0W68_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075421.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="K0W68_003996"
     CDS             1257..1376
                     /locus_tag="K0W68_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBI3075422.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="K0W68_003997"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="K0W68_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBI3075423.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="K0W68_003998"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="K0W68_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBI3075424.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="K0W68_003999"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="K0W68_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBI3075425.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="K0W68_004000"
     CDS             4176..4544
                     /locus_tag="K0W68_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HBI3075426.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /locus_tag="K0W68_004001"
     CDS             complement(4652..5053)
                     /locus_tag="K0W68_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBI3075427.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="K0W68_004002"
     CDS             5295..5588
                     /locus_tag="K0W68_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBI3075428.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="K0W68_004003"
     CDS             5660..6319
                     /locus_tag="K0W68_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBI3075429.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="K0W68_004004"
     CDS             6411..6857
                     /locus_tag="K0W68_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBI3075430.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..>7623)
                     /gene="hlyE"
                     /locus_tag="K0W68_004005"
     CDS             complement(7064..>7623)
                     /gene="hlyE"
                     /locus_tag="K0W68_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HBI3075431.1"
                     /translation="VLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEK
                     SSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQS
                     FFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAA
                     KKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1957 a         1714 c         1772 g         2180 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc
     2281 gcgtaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ctccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 cct
//
LOCUS       DAETQN010000179         7492 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.181, whole genome shotgun sequence.
ACCESSION   DAETQN010000179 DAETQN010000000
VERSION     DAETQN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.181"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="K0W68_004006"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="K0W68_004006"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HBI3075432.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVTYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="K0W68_004007"
     tRNA            647..722
                     /locus_tag="K0W68_004007"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="K0W68_004008"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="K0W68_004008"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HBI3075433.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="K0W68_004009"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="K0W68_004009"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBI3075434.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="K0W68_004010"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="K0W68_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HBI3075435.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="K0W68_004011"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="K0W68_004011"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBI3075436.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="K0W68_004012"
     CDS             complement(5917..6249)
                     /locus_tag="K0W68_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBI3075437.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="K0W68_004013"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="K0W68_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBI3075438.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1824 a         2029 c         1870 g         1769 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat gtcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaatacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacag tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgca
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac ag
//
LOCUS       DAETQN010000180         7430 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.182, whole genome shotgun sequence.
ACCESSION   DAETQN010000180 DAETQN010000000
VERSION     DAETQN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.182"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..100)
                     /locus_tag="K0W68_004014"
     CDS             complement(<1..100)
                     /locus_tag="K0W68_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HBI3075439.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAEE"
     gene            complement(125..1225)
                     /locus_tag="K0W68_004015"
     CDS             complement(125..1225)
                     /locus_tag="K0W68_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBI3075440.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(1613..2359)
                     /gene="cutC"
                     /locus_tag="K0W68_004016"
     CDS             complement(1613..2359)
                     /gene="cutC"
                     /locus_tag="K0W68_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBI3075441.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(2373..2939)
                     /locus_tag="K0W68_004017"
     CDS             complement(2373..2939)
                     /locus_tag="K0W68_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBI3075442.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            3155..4891
                     /gene="argS"
                     /locus_tag="K0W68_004018"
     CDS             3155..4891
                     /gene="argS"
                     /locus_tag="K0W68_004018"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBI3075443.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            5068..5430
                     /locus_tag="K0W68_004019"
                     /pseudo
     CDS             5068..5430
                     /locus_tag="K0W68_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(5434..6504)
                     /locus_tag="K0W68_004020"
                     /pseudo
     CDS             complement(5434..6504)
                     /locus_tag="K0W68_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(6497..>7430)
                     /gene="flhB"
                     /locus_tag="K0W68_004021"
     CDS             complement(6497..>7430)
                     /gene="flhB"
                     /locus_tag="K0W68_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HBI3075444.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1920 a         1947 c         1776 g         1787 t
ORIGIN      
        1 tctcttctgc ccatgcgggg agcggtgctg ccgatgtcac cacctgcgct ccagcggcaa
       61 taccgctgtg tttaataaat tcacgtcttg ttaatgtcat aacttcctcc ctgatcaacg
      121 aggatcactg tttctcggta atatctttgg cgttgtactg gaaataccgc gttaaaatgt
      181 ccagttcgtt ttcgctcatg ctggttcgtg cccccattcc tttggcaatg gacggccacg
      241 cattgacggt gtaatggtcg gcggcaatag gggcatgaca accagcgcaa taggtatcgt
      301 caagtttttc agcgtattgc catagcggtt tacggtccgc taatccggga tcggtaagcg
      361 caccctgtaa agacgcctga cgccattgat tgccgtattc gtcagcctgc cattccccgt
      421 ttaccgtgag cgccttgata ccttcttcac ttaatgtggc tagcgccagc cgttgacctg
      481 ccgccaggta gagcgtgttt tcactgccct gcatttgata accctgcaac agaacgatcg
      541 gctgtttgcc actggcatca acgacggtga gatcggtacc aggattcacg gtagccagct
      601 cgcctatgcg agaagttttg aaaggataaa tatgtgcgcc attagtcact gaagtagcgg
      661 cctgactttc cagctcatgc gccgcgttgt catccatttt tatttctggc ggaaaatggg
      721 caatgccttt atgacaatcg atacaggttt cgccgccctt ttgtgctttg ttatgcattt
      781 tctgcgcaga ttcactttgc gaggcaatat ccatggcatc aaaagaatgg caactacggc
      841 acgttgcaga gtcagttgct tttaattctt tccatactgt ttcggccatt tcctggcgat
      901 gagtttcgaa cttatcgtca ctgtctattt tgccgctaac aaattcatga taaatatctt
      961 tagatgcttt taatttagca aataaataat ccatccctga ctttggaata tggcaatctg
     1021 cacattccgc acgtatccct ttctggttcg aaaagtggac agttccctga tattcctcaa
     1081 aagatttact catcgagtgg caggaaaggc aaaatgctgt atccgacgtt ttatgtaagg
     1141 ctttttgcgc cagcaataac ccgccgccac caaccacaac cgctatcagc aaaaacaata
     1201 acccaatgcg ttttttccct cgcatagatt cctctaaata aattccagaa caccggaatc
     1261 attgtataat taaatacaca acgaattaac cgcttgcata actcggcaca acactgcctg
     1321 aaataatcga taaagaatat gatttattac aatgtaatca ttaattgcta aggaataacc
     1381 cagttgctat ttagaatata tatttatcag ttttagcaat ttaaatccca taatcaatgt
     1441 gaatataaaa aatattatta gcacaccgct atgttcactg tcacccgctc aacagatcac
     1501 ggttatcgtt cgttttttat actgctcagg gataaaaaaa ggcccctgtt gaaattgcag
     1561 gggcctggta cgagcaagca tcatattggg cgacatgatg caacggtaaa aatcatttgg
     1621 cctgatggcg ttcaatgatt cctttcattt cagcaaccgc cgccccgtct acgatataac
     1681 gcgaatactc gtccgcgtgt tcatctgatg acatggacaa tccttgatta cgataacgca
     1741 tcggtgaggc ttgccacgct cccgcggagc tatggacttc cagcactccg gcatcgagga
     1801 agtggtgcag gttttctgca cggactcctg ctccggccat aatgattgga gcatcacgat
     1861 gggcaataag ttccataatt tttgataaac cttgcagtgc gtctgatttt tgccctgatg
     1921 tcagtacccg ggcaatgccc aattccgcaa gattattgag tgtatataaa gggttagcgc
     1981 acatatcgaa ggcgcgatga aaagtcactg ccagcggacc ggcagcagcc attatttttt
     2041 ccattcgtgg catatcgaca ttcccgtcaa catcgagaac gcccgtcacc agtccaggaa
     2101 aacctaattc gcggaccgtg cgcacatcct caagaatggc ggcaaactca ccgtcgctgt
     2161 agcaaaaatc accaccgcgt gggcgaatta tcggatgcac agggatcgtc acccgctggc
     2221 gcacggattt cagtaccccc aacgacggcg ttaagccccc ctcttttggg gctgcgcata
     2281 attcaactct gtctgcgccg ttttgctgcg ccgttagtgc acattccatg ctgtaacagc
     2341 aaatttccag taatgccatt tttgctcctt aattacgctg actgctcgct ggcaacgatc
     2401 tcttcaatgg accacggatg aaacttaata gttgttttgc catcggtgac cgccagcgtc
     2461 ggattcggta atcgctcatg ctcacctttt ggtgaactgg ttttaactga gatccccggt
     2521 aggctgagtc cttcatcaga aagcaatgcc agcgcacggg cattcagcgt ttctggatca
     2581 cccagcagaa cgatttcaat atgttcccaa ccttcgtgtg ggtaacgttt ttccccgggc
     2641 cacggtagct ccacaataga aaactgccag tgcgcaacct gtaccggttc atgcaattta
     2701 aacagacaaa tcggtctgcc attgatcata ttttctgaca aaagctcgcc acactgttca
     2761 aacccgcgac gccagcgttc agcagtggcg ttttgatggc aacgcaaaga aatgtgatcg
     2821 gcagtcagcg gagtgatatt caaccccaga cggagggaaa gttcatctaa tgcgtgggta
     2881 aatcgcggta aatccgatgc aatatcctgc agctcgtcga tagattgcca gttcgccata
     2941 atcactcttc gtctttcagt aaaagcgtta atttaccctg ttgccctgcg ccaaccaacc
     3001 gctgatttca cgccgcttct gatgcaatag tgaaaacggc aatacgccac gcgcacgttg
     3061 ctgacgaaaa cagccatttg cagtatactc ccgccctaat ttctttaact ggtgcgggca
     3121 atttttgctc gcttcatcaa tgtaaggtat tccggtgaat attcaggctc ttctctcaga
     3181 aaaagtccgt caggccatga ttgcggcagg cgcgcctgcg gattgcgaac cgcaggttcg
     3241 tcattcagca aaagttcagt tcggcgacta tcaggctaac ggcatgatgg cagttgctaa
     3301 aaaactgggt atgacaccgc gacaattagc agagcaggtg ctgactcatc tggatcttaa
     3361 cggtatcgcc agcaaagttg agatcgccgg tccaggcttt atcaacattt tccttgatcc
     3421 ggcattcctg gctgaacatg ttcagcaggc gctggcgtcc gatcgtctcg gtgtttctac
     3481 gccagaaaaa cagaccattg tggttgacta ctctgcgcca aacgtggcga aagagatgca
     3541 tgtcggtcac ctgcgctcta ccattattgg tgacgcagca gtgcgtactc tggagttcct
     3601 cggtcacaaa gtgattcgcg caaaccacgt cggcgactgg ggcactcagt tcggtatgct
     3661 gattgcatgg ctggaaaagc agcagcagga aaacgccggt gatgaaatgg agctggctga
     3721 ccttgaaggt ttctaccgcg atgcgaaaaa gcattacgac gaagatgaag agtttgccga
     3781 gcgtgcacgt aactacgtgg taaaactgca aagcggtgac gaatatttcc gcgagatgtg
     3841 gcgcaaactg gtcgacatca ccatgacgca gaaccagatc acctacgatc gtctcaacgt
     3901 gacgctgacc cgtgatgacg tgatgggcga aagcctctac aacccaatgc tgccaggaat
     3961 tgtggcggat ctcaaagcca aaggtctggc agtagaaagc gaaggggcga ccgtcgtatt
     4021 ccttgatgag tttaaaaaca aggaaggcga accgatgggc gtgatcattc agaagaaaga
     4081 tggcggctat ctctacacca ccactgatat cgcctgtgcg aaatatcgtt atgaaacact
     4141 gcatgccgat cgcgtgctgt attacatcga ctcccgtcag catcaacacc tgatgcaggc
     4201 atgggcgatc gtccgtaaag caggctatgt accggaatcc gtaccgctgg aacaccacat
     4261 gttcggcatg atgctgggta aagacggcaa accgttcaaa acccgcgcgg gtggtacagt
     4321 gaaactggcc gatctgctgg atgaagccct ggaacgtgca cgccgtctgg tggcagaaaa
     4381 gaacccggat atgccagctg acgagctgga aaaactggct aacgcggttg gtattggtgc
     4441 ggtgaaatat gcggatctct ccaaaaaccg caccacggac tacatcttcg actgggacaa
     4501 catgctggcg tttgagggta ataccgcgcc atacatgcag tatgcttaca cgcgtgtatt
     4561 gtccgtgttc cgtaaggcag aaattaacga agagcaactg gctgcagctc cggtaatcat
     4621 ccgtgaagat cgtgaagcgc aactggcagc tcgcctgctg cagtttgaag aaaccctcac
     4681 cgtggttgcc cgtgaaggca cgccgcatgt gatgtgtgct tacctgtacg atctggctgg
     4741 tctgttctct ggcttctacg agcactgccc gatcctcagc gcagaaaacg aagaagtgcg
     4801 taacagccgt ctgaaactgg cacaactgac agcgaagacg ctaaagctgg gtctggatac
     4861 gctgggtatt gagacagtag agcgtatgta atcgattttt cgtgagagta aagcctgatc
     4921 agggttacct gatcaggctt ttttattgcc atctaaatgt attctgaaaa tggacatgcc
     4981 attgttttct gactgttgga taagaggcca gaagcgtaat atccggcccc agggaaacga
     5041 taacggttga atttaaggaa taccgcagtg tttaaatttc ttgtattaac attaggcatt
     5101 atctcttgcc aggcttacgc agaagatacg gttatagaaa acgaccatga catttcagcc
     5161 atcaaagatt gttggcaaaa aaattcagat gatgatactg acattaacgt gatcaaatca
     5221 tgcctgcgac aagaatacaa tctcgtcgat gcgcaattaa ataaagccta tggtgaagct
     5281 tatcgttata tagaacaagt gccacgcaca ggtgtaaaaa aacctgatac cgaacaactt
     5341 aacttgctta aaaaatcaca gcgagcctgg ctggatttta gggacaaaga atgtgaatta
     5401 atcctttcaa atgaggacgt tcaggattta atgtacatcg ttccacgtcg cttcgacaac
     5461 ggtattattc tctgccattt ttaccggttt gggttcggca ggcgcttttt gttcgcgtcc
     5521 gcgtatccac caggccagcc cgagcaatcc ggcagtgaac agcaaaaata ccaggttcgg
     5581 cattccaggc accaggccga gtaaaccgag cacggcggcg cttaacaaca taacgcttgg
     5641 gttactgaaa agctgattca ccatctgctc gccaacatcc tgatcggtgc tgacacgcgt
     5701 aacgatgacc cccgcggcgg taaaaatcac cagcgccgga atttgtgcca ccagaccatc
     5761 gccaatggtc aatagcgtat aactttccgc cgcgtgtccc atgctcatgc catgttgcag
     5821 cacgccgacc agcaacccgc cgacaacgtt aatgaccatg atgaggatcc cggcgatggc
     5881 atcgccgcga acaaacttac ttgccccgtc cattgagccg taaaaatcgg cttcctgagt
     5941 cacttcggag cggcgttttt tcgcctcatc ttcaccaatc aatccagcgt taaggtcggc
     6001 gtcaatcgcc atctgcttac ccggcatacc atcgagaaca aagcgcgcac ccacttctgc
     6061 gatacgcccg gcacctttgg taatgaccat aaagttgatg atcacgagaa tgacaaacac
     6121 cacgataccg atagcgaaat tgccaccaac gaggaagtga ccgaacgctt cgaccacctt
     6181 ccctgccgcc gccgcgccgg tatgcccttc catcaaaatg atacgggttg aagccacgtt
     6241 aagtgccaga cgcaacaacg tagtaaacaa cagaatggtc ggaaacgcag caaactcaag
     6301 cgtgcgctgg gtaaacatcg ccaccagcaa caccatgatc gacaaggcaa tattgaaggt
     6361 aaacaacagg tcgagtatga atgcgggcag tggcagcacc atcatcgaca agatcaacag
     6421 gatcaaaatc ggtccggcaa ggatctgcca ttgtgtcgat ttcaggtttg cgggcaggcg
     6481 cagcatcgcg gccagattac tcatgggtcg gtttctcgtt aataaaatcc agggcttccg
     6541 gcaccggaag atgagtaggt tgtacagggc gctgtccacc agccagacgc cagcgtttca
     6601 gttgccagac ccaggccagc acttccgcca ccgcggcgta cagttgaccc gggatttgtt
     6661 gaccaatctc cgcatgtcga tacagcgctc gcgccagcgg cggcgcttca agcgtcggga
     6721 cgttattttc agcgccaatt tcacgaatgc gcagcgcgac cagccctgca cctttagcga
     6781 ccactttcgg tgcgctcatt ttgttttcgt catactgcaa cgctaccgaa tagtgggtcg
     6841 ggttattgac aatgacatca gctttcggca catcggccat catccgacgc cgtgcagcag
     6901 ctcgctgcat ctgacggatc cgccctttaa catgagggtc accttcgctt tgtttgaact
     6961 catcacgaat atcctgccgc gacatacgca gctttttcag gtggctgaag atttggaaaa
     7021 agacgtcaaa tcccaccatt ggaatgacac caagcaccac cagcagtgcg catagcccaa
     7081 ccagatccat cgcattaccc atggcggtag tcggagactc ggccattaag cgcatcatct
     7141 gcggccagtg atgccagaga taaaaccccg tcacgctgcc aaccaggatg gttttcaaaa
     7201 ttgccttaag caactccgcg ccagtctgtg ccgagaacat ccgtttaatg cccggtagcg
     7261 ggttgagttt ggaaaacttc ggctgcaagg atttgccgct aaataccagc ccccccagca
     7321 tgaccggaga aatgagcgcc accagcacca cgccgctaat cagcggcagc agcgccagca
     7381 tggcttctct gatcagcaga ataatctgcc cgaggatcag attcgggtca
//
LOCUS       DAETQN010000181         7408 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.183, whole genome shotgun sequence.
ACCESSION   DAETQN010000181 DAETQN010000000
VERSION     DAETQN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.183"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..157
                     /locus_tag="K0W68_004022"
     CDS             <1..157
                     /locus_tag="K0W68_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HBI3075445.1"
                     /translation="FINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAV
                     SGKRFER"
     gene            complement(510..1250)
                     /gene="phoN2"
                     /locus_tag="K0W68_004023"
     CDS             complement(510..1250)
                     /gene="phoN2"
                     /locus_tag="K0W68_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HBI3075446.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(1579..2445)
                     /gene="ospB"
                     /locus_tag="K0W68_004024"
     CDS             complement(1579..2445)
                     /gene="ospB"
                     /locus_tag="K0W68_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HBI3075447.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            complement(2695..2814)
                     /locus_tag="K0W68_004025"
                     /pseudo
     CDS             complement(2695..2814)
                     /locus_tag="K0W68_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            3058..3430
                     /locus_tag="K0W68_004026"
                     /pseudo
     CDS             3058..3430
                     /locus_tag="K0W68_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3992..4939)
                     /gene="icsP"
                     /locus_tag="K0W68_004027"
     CDS             complement(3992..4939)
                     /gene="icsP"
                     /locus_tag="K0W68_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HBI3075448.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIDYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(6321..6473)
                     /locus_tag="K0W68_004028"
                     /pseudo
     CDS             complement(6321..6473)
                     /locus_tag="K0W68_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6529..7221
                     /gene="ospZ"
                     /locus_tag="K0W68_004029"
     CDS             6529..7221
                     /gene="ospZ"
                     /locus_tag="K0W68_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HBI3075449.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYGK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRDEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHALCELPEISCKIFPKENSDFLYIIVVFRNDSPQGELR
                     ANRFIELYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMND
                     RLSQIKARMPVT"
     gene            complement(7245..>7408)
                     /locus_tag="K0W68_004030"
     CDS             complement(7245..>7408)
                     /locus_tag="K0W68_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3075450.1"
                     /translation="VGYVSFREAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWK
                     NSNSVASFC"
BASE COUNT         2251 a         1433 c         1409 g         2315 t
ORIGIN      
        1 ttttatcaat aatggattgg ttgatgtaaa caaaaaattt caaaaagcaa atagtgggga
       61 cactatgctt gacaatgcaa tgaaaagcaa agattcaaaa atgattgatt ttttattaaa
      121 aaatggagcg gtatcaggca aacgatttga gaggtgacct aaaatatatt taggttgtgc
      181 cctgaatctg aagttgtcat ttctgttata ttgatcgaca taggggatgg tgcagaccgt
      241 tcccctgcaa caccacattg catgtggtga acggaaccgc cccgagtttc ctagagaatg
      301 ttttatctgt gaactcaggt tgccagatca tcgtttccga tggcagcata ataagctttt
      361 tctgcttctg ccggagggat atgtcccagc ctttccagta atcccttaaa ttcaggggta
      421 aaacgtggtg tgcccataga ctcctcctat gctcatatag ggtagatttg tctatgggcc
      481 ctccatagcc tgatacaggc tgtccagctt tatggggtca gttcattggt aggagtattt
      541 aattcttcaa actctttttt ggcttcgcta aggcttttgg taaattcagg tgtattatga
      601 agtactgcaa caaccgatgc tcccattaaa cgcccagcct ctacatcgct ttgccaatgc
      661 gcaccgcaga tgacccgact ttctccaaac tcatatccac gtcgaagtat ttccgcttta
      721 cgttgaggat taatctccgc aagtatcagt gctactgccc aaccaaagga tgcatgacca
      781 gagggataag agccagtgat agccatgttc tcatctttat caggtgtaca ggttgcgtct
      841 ttataaataa cgaatggacg aactctttta taatattctt tggcgttacg cacggcgtaa
      901 tcatggctat cctgtagtac ttgtgttaac aactgatata agattggggt attttccctt
      961 gaaatatcca tgccataagc atctgaaaat gcaagaccaa aattctcgta gtatgcatct
     1021 tcgctagcga gacgtacacg attagcatcc cggagcgagc ggccgaattc ataatgagct
     1081 ttgtcagcct gaaatactac tgaatcctct gccggaggcg gcggaagtat tgacaaactg
     1141 tctggtgaag tttgttgagt gagaaaacct tctgccttca gagcatttgc tgaggggata
     1201 aaaatcatat ttgtagcaat acaaaaaaga agaaagtttt tggttttcat aatttatttc
     1261 cttagcataa ttaattgatg attttggtca gctgtaaaaa acgagctgtg ttacaaaaaa
     1321 cacaagtcat gcggaaaaat ttgaacagag tgatggaaaa acaaaaaata gtcttatatt
     1381 aaagtaataa cattattttt catattcacg aaaaataatg tggtaaaaga atgatgtttt
     1441 ttatctattt gttcgtatcg ttttgtctct gtattatgat gatttgtgat tttccctcat
     1501 agggaagcac ttttctacta aagataaaat aacttcatct taaatgcaaa caattaacta
     1561 taggttaaaa attttatact aatccagttc tttattaata ataaaagtct ttttctgaga
     1621 gcttcttttt actgtatgct ccggatcagc aggaattgaa gttgaacgat aatgtgagta
     1681 gggaaaaagc tcttgaccat agtgaactcc atatccatga tagccggata tttttagacc
     1741 atcactcaat gactcatcgt tttcaaggtg tttttttatc tgctctagca aagattcttt
     1801 ttccttaaaa aaaggcagag agttaaggat acaagaaaga ctttcagcag gggcattgtt
     1861 aaaattttta ggagccactt tatcagcgga gccgcatgaa gtaaaacgga tatctttcac
     1921 atttagaata ccacagtctt tcattcttga aatgattgtt tgtacatcaa taagttccga
     1981 accaaggcct aattgatgag aaccaggact accgtgccca agaatataaa ccttattata
     2041 ctctttaaaa tcagtatcgt ttactgtttt tattttcgct ttttttacag aaaaataagc
     2101 agcagcattc aatgcgtcat catcattatt tattactatt ggttcactct gtggtagcca
     2161 tgattcagcc atttttagaa aatcattttt acttttttca tttttaaaaa cataatcata
     2221 taacatcttg ttcttaaata ctcttggtat ttgttgtccc ataatagata gaacatcatt
     2281 gctatcacaa aaacctttct ctcctaaaaa aaccaatgct gcttttgggt gagtacatga
     2341 tgaatttttt acccttttta ttatatgtgc aaatgcaata tctgatatgc tttcattctt
     2401 tttattgact attctacagt atggtctaac accatctaaa ttcataccta catgactcca
     2461 tatattgttt tataaacatc atagtaaagg gtgttatatt ttcatatagt atgcaagctt
     2521 ctttgtatac tatgatttaa aaaaacggaa caaaaatgca attatattct ggcattgttt
     2581 aatgctgttt gtctttttta ttcattattt caatgaaaga atttcaacta caaagttaat
     2641 tgatcctacc tacataatat agacacagta cctaaagtac cgtggagatc atccggggaa
     2701 gccccgggtc tgataccagt gcaggtagac cgctatcttg ttccggtaat gttctgccgt
     2761 tctttacggc atcacctcta gcagaaacat cgcgtaactg cgccaagtat gatgaacttc
     2821 tcttaccgct gcgatctggt tcaacaatat gcagatggta cacaacctct ttaaccagcc
     2881 catccggatt attcagagcc gcttccgcaa tccggaataa aagtgctgtt ttaccgtgaa
     2941 ccctctgaaa atcagcgagc agtgttttaa ccagccgacg ttcagagcgt actgacagac
     3001 ggtggacaat ttgaataaac agttcaatca gaccgtagat gctctcccgt tggcgacatg
     3061 ctgggagcgg tggaacaccg cttcggcaac gagcttccgg cgtctccagt gaagtgactg
     3121 acggataatg gttcatgcta ccgggctaat gaaacacgcc agttcacccg gatgttggga
     3181 ctttgttgaa ccgaagaaca cggcggtgcg gagtccggag agtaacagaa tagcaaagag
     3241 cttcgtgaaa acaataaagt gtgactacat cagtatcatg cccaaacctg acgggttaac
     3301 ggcagcaaag aaccttgcag aggcattcga gcattataac gaatagcatc cgcatagtgc
     3361 gctggattat cgctcgccac gggaatatct gcggcagcgg gccaatgata acaggtgtct
     3421 ggaaatatag gggcaaagcc acccacgaac gatccagcat ataatcaaga aattagattt
     3481 catttaacat aagatagatt atgtgcacca agaggcgttt ccttaatttc tgaatttaca
     3541 ggctcttttg ctctctttgc tatgctgtgg atttggaaag atagagtgct ggctgattat
     3601 ttaggtattt tcatcagcat gtccctgata gcactgttcc atcagcgctt tcaggcgata
     3661 atagtagacc ttgggtagcc tctgcccatg tgtaacttga taccccgaga acagccacga
     3721 tgatctaagc ggttcttatt tctccggtct cagggtcggt aatgccaaca gtaagtccac
     3781 agtaatcaac gaagagtttt ttgaaccgcc ccggaaatcc tggagactaa acgccctgag
     3841 aaagaggtaa acagaatgac taaaaataca cgtttttccc ccgaagtccg tcaacgggca
     3901 gttcgtatgg ttctggaaag tcagggcgaa tatgactcac aatgggcggc aatttttagc
     3961 catgaaaacg taatcaactc ggtgaaccaa ttcaaaaaat atactttata cctgcggaag
     4021 tgagaaaacc aatatattca atacttgcac tatttttaat ggtgccagat atattggtac
     4081 tttgctcata aagagatgta tcaccttttt tattagaaat gtaatttctg gagccttcta
     4141 tataaaattt tgcactgggg gtgacatagt atccaatatt cgcagcaaca gagcagtaat
     4201 tttggttttt tatttcatct ttaaaaattg tctcagtctg atagtgttta tctgtatcag
     4261 atgaacgaac ccaaccacta tatttcagtt ctgctccaaa ctcaaaattc tcatggcggt
     4321 aattagcagt taatccaata taaggtattt tgaagagctg tttgtaatct attgttcttt
     4381 caccactagg atgtgcccct tttttattcc tgttcccccc gttttcacta taaatataac
     4441 tccctcccat tgcattaaaa ctgtaacgac tctcttggta acctgctatt agtccaagtc
     4501 gataatccaa attatttaat aaccaacctt taagattcag ctcgaattcg ttagcatcac
     4561 ggagatgcgt attgggatgc cagctctggt cggtccatac ctgaggggta ttggaattgt
     4621 tccagtcttt atcaaccatg cttgctttct ggttacctaa agtagtccaa ccggaaactc
     4681 cgaaagacac ttttggaatt agcttccaat cgatgccacc tctaacaata gtcgcattac
     4741 tgtatttcca gtccagttga ctaatttttc tccccccttc cttgggatga taaacgcgtt
     4801 cttttgtttt gccactcaga gatcctagac taatatccgt actgatgttg tccggtatga
     4861 aaagtggata gttagtggta gcatgtgtag taaagatcgc tggtacacaa agtgcaagta
     4921 caaggaattt taatttcatc gagaactcta cttttttggt tgaaatgtcc atgatcttct
     4981 tactttataa gataaacgtc aatgccaagc attattttaa ttaaatattt gtttatgtta
     5041 ccaagaacat atcttttatt ataatattta ttgaactttt gtaaaaataa aaaaaatacc
     5101 tcattgtgcg aataaagtaa cgggggccat agatttctct acctaccttt tggtgttcat
     5161 acctgaggct gatagtttca ggattccgcg acaattctac tcaccttctt gttctctcgc
     5221 caatatgaaa tcaatcagaa aagcgttcca taagaacaac gataagacaa taaattgtca
     5281 tcacggctgc aatagccaac gtgcctttca gtaccaacat gacaacctcc tgtatttgac
     5341 gtaacgtgtg ctgataaata tgtatttgta tctcaactga ctcaatacaa cacttaagta
     5401 tagtttttca gattttgttt ttcccgtgaa atattatgga aatggcagaa gcaatacatt
     5461 gaattccgat tcgagtgaat acggctagcg ggcacctcac tttagcactg aagccatcaa
     5521 cttttatttg acttatttca atggaaagct ttctcactat cagtacaggt agttcccatc
     5581 cttttaaagt cgagagtcag actgcgacta atacagttaa agtgaccaaa ctcagttatc
     5641 ctctacacat tttacctcat gtttacttgg acaattcatt ggtgctttta taaaagaata
     5701 aatattctac taatttaatg catcacctaa aatagtattt tttataatag aactgtatct
     5761 atagtatatt attaaagctt aattatttat tatgctttaa tgtggttgtc cgattaagga
     5821 ctaatccttg aaaacgccgt atagggcggg agttcactct attgcgataa cgtaaccgta
     5881 caatattgtg cgtttcccaa attgaccact actgaactat tctgggattt aaatgcaata
     5941 aaatggttgg ttgaaggtcg tggtgatgat gttagtcctt ttgagaatat tccagtgcca
     6001 gatcttactt ataataaaga ggcttggcag tttggataag ctattgtcct tattccacta
     6061 atacaaactt tttgtaaagt atattaaata tacttcattt catactgaaa tccccggaag
     6121 tattcaatca aacaattaca ccataaaagg aagagggagc cccaattaag ttatcaagca
     6181 tgtttatgat acctgcaggg tataaatttt ctggctgcaa gcagacacat ataaaacgtc
     6241 tttattgatc ctacccacat aatatggacg cagtcgtaag tgaggctctg tgagcctacc
     6301 cacgtaatat ggacacaggc ctaagcgagg ttctggtttt caaattgttc cggactgagg
     6361 ccgccacacc aacggtgccg tcgccagcga ttgtaatcgc actcgatata attaaacact
     6421 gttgcccgca ctatttcccg atagataaag tgttctccgt ggagacattc cacttaggaa
     6481 caattatatt gttcatggta atgtttatat ttgagtatag agattaaaat gattagtccc
     6541 atcaagaata ttaagaacgt atttccaata aatactgcaa atactgaata tatagtgaga
     6601 aatatttatc ctagagttga acatggttac tttaatgaat caccaaacat atatggcaag
     6661 aagtatatct ctggtataac cagaagtatg gcacaattaa aaatagagga gtttattaat
     6721 gagaaatcca gacgacttaa ttatatgaag acaatgtatt ctccatgtcc ggaagatttt
     6781 cagcctattt ccagagacga agccagtaca ccggaaggaa gctggttgac agttatatct
     6841 ggaaaacgtc ctatggggca attttctgtg gatagtttgt accatcctga cttacatgca
     6901 ttatgtgagc tcccagagat tagttgtaag atcttcccta aagaaaatag tgattttttg
     6961 tatataattg ttgtattcag aaatgattct ccccaaggag aactacgggc aaatcggttt
     7021 atagaactat atgatataaa aagagagatt atgcaagtgt taagagatga gtcgccagag
     7081 ttaaagtcta ttaaatctga gattattatt gctcgtgaaa tgggagaact ttttagttat
     7141 gcatcagagg aaatagatag ctacataaaa caaatgaatg acaggctttc tcaaatcaag
     7201 gctcgaatgc ctgttactta atgtatatta attgtatgaa gtggtcaaca aaaactggcc
     7261 accgagttag agtttttcca gtatcgattt tccgattcgt ttgggggtaa cccaccgtta
     7321 tattcgtgcg gtcttagtgc gctgtaatat ccaacgatat agtccgttat ggcgtgagct
     7381 gcctctctga agcttacgta acccacca
//
LOCUS       DAETQN010000182         7297 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.184, whole genome shotgun sequence.
ACCESSION   DAETQN010000182 DAETQN010000000
VERSION     DAETQN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.184"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..4273)
                     /gene="traI"
                     /locus_tag="K0W68_004031"
     CDS             complement(<1..4273)
                     /gene="traI"
                     /locus_tag="K0W68_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBI3075451.1"
                     /translation="MMSIAQVRSAGSADNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVTNVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKRKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     MYTDLLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLT
                     LRNAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDHFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     VGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLERVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRK"
     gene            4355..4582
                     /gene="vapB"
                     /locus_tag="K0W68_004032"
     CDS             4355..4582
                     /gene="vapB"
                     /locus_tag="K0W68_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HBI3075452.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            4582..4980
                     /gene="vapC"
                     /locus_tag="K0W68_004033"
     CDS             4582..4980
                     /gene="vapC"
                     /locus_tag="K0W68_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HBI3075453.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(4989..7145)
                     /gene="traD"
                     /locus_tag="K0W68_004034"
     CDS             complement(4989..7145)
                     /gene="traD"
                     /locus_tag="K0W68_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HBI3075454.1"
                     /translation="MFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCT
                     TLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVI
                     CLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVARMLKKDGRDSDIRIGDLPII
                     RDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVIYDRSGEFVKSYYDPSIDKIL
                     NPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQGSGRTIFAEAAYLMRND
                     PNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIEKTAISIRAVLTNYVKAIRYL
                     QGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAM
                     GENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAA
                     TLFDVMNTRAFFRSPSHKIAEFAVGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMER
                     QTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQERPKVAPEFIPRDINPEMENRLS
                     AVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPQQPQQPQQPQQPQQPQQPQ
                     QPVSPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAW
                     QQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(7171..>7297)
                     /locus_tag="K0W68_004035"
     CDS             complement(7171..>7297)
                     /locus_tag="K0W68_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3075455.1"
                     /translation="RHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         1512 a         2243 c         1869 g         1673 t
ORIGIN      
        1 gtttccggtt accttccgtg ccttcaagcg cgatggcaaa ggtttcactc gccccctgtg
       61 caccgtgggc ggtgatggca taggccaggt caatatgttg ctctgcccgc tcctgaccgg
      121 ggcgaatcac acgggtctgc tgaccgtcag aaagtgtgac actgtcaccg gaaaccgctg
      181 tcaccgtcca gacgctgttg gccacataac cgcgctcccg gtcactcttc gtgaagcgta
      241 tcctgtcacc ggtccccacc cggatggtgt ccggggtgta cagcgtgacg ccttcagcca
      301 ccgcctcccg gggcgaaatc agccgcgtgt taccttccgc atcctgcagg gttatcagcc
      361 cgtcatcctt gctgataccg gcaatccggt gatacacatt atccaccagg gcaagggcat
      421 ccgggttatt ctcccaggtg gagagacgac gcagctcccc gtcgcgtata ttcgctgtgt
      481 tcaggacagg caccatgacc tgcacctgcc ccagttctcc ggccttttcc cgtgcatcat
      541 gaatcatgct gttcagtacg cgccgatcct cattcaggtg cgtgacaatc agcgtctgct
      601 cccgtgcttc cggtgtcctg ccggtatagt cgcggacaat ggcttcatac agtgtcatgg
      661 ggacatccgg aaaagcctcg cctttcagca tcgccttctg ctgcgcttct gccagtttcg
      721 cttcctggct gtgactgaac tccgtcacgg agtgctccgg tgcccatgcg ccctccagac
      781 gtggcacctg agacggtttc acacgctcaa gcccggacag cgccctttcc acatcccggt
      841 taatcaggct gtataccgcc tcccgcagtt ccggcgtctg acgcacaatc tccttcatga
      901 tgaccacatc ggcagcactg cgcgtctgct ggagacggaa aggctgacca ggcgcgatgg
      961 cctgcagctg gtccgtgtca ccgctggcca cagcccgacc gccaccgacc gcaatcaggg
     1021 cgtatgcccg tgccatgtcg gtattgccca ccattgagct ctcatcgagc aggaacagcg
     1081 tgttgctgaa atccggcgtt tctccgctgc gctgctgcag ctgcgtgtca tgcagaaagg
     1141 acgccagtgt ctgcgcatcc acgccggcgc tgcgcatctc cccgaccgcg cggtgcgtgg
     1201 gacccagccc aacgacacgg ggacgttcac tctccggcag catgttcacg gctgacatca
     1261 ccgcccggaa ctgtgtggtc ttacccacac cggcatagcc ctgcaccacc gtgaaatggt
     1321 cggacgtttc cagtatcata cgggtggcgg cgcgctgtcc tgatgtcagt ttctccatga
     1381 gttcgccagg tactctctcc atcagcggcg tgaccgcctc cttaccttcg agaatatggc
     1441 gaagaatgct cttttctgcc tcatacgacg cacgggaaac cagcaggcct gtgccatagc
     1501 cttttgccac atccacatac agtaaatcac cccgttttat ctgcgcattg atttcccctc
     1561 ccaggtcagc aaaaccggtt ccttctgcag caaacgactt cgcctctgtc agcaggtcca
     1621 ccatgctgaa ggccaggttt ttactttcca gcacaggaag ggccagatga atggcctgct
     1681 gtgccggcgt gtgaagcccg gctttctgca gactgatagc ggtttccagc aatgtctcac
     1741 cggcacgcgc ctttatctgc tcagaaacca ccgtaaagga gggatggcgg gcaagttttt
     1801 ccgcagtacg ggtttcatcc agtgaggaat acagccggac atcacgacca ctgcgggcca
     1861 gaccgttcag ggtggcattg tccattgcca tctgtgtgac ggaggcaaaa accgtcgcac
     1921 tgtcgctgac ggaatgcccg ggcgtttcca cccagccgtt ctccagcttc agtgccgtga
     1981 acggtgaatc gctcacaggc agggtggccg gttcagcccg tcccggcaca acaaccgtca
     2041 tcgcatcttc actgacggat gccacctgca ggcggtcacc gccggagacg cggagtccgg
     2101 gaattttccc tgtcaccctc agacgctcac cgtctgccac cggcattttt tccggccgga
     2161 acagcgacca gctgctgtcc agggaagaaa tacgcaccac ctgcgtttcg ccctgcgcat
     2221 tccgcagggt caggctgtga ctctgcgccg tcacacgttc tgtcacatag cggtcatgac
     2281 tgcgtgtctc cgggttccac tgctccatca ccattcccgg gcggtacata tcccgcagat
     2341 aacggctccg gctgtccagc cagaccggtg acagggcggt catggttacc tccgggtgtc
     2401 cgagcacgcc ctgtgttttc agctcactgc gaatggcctg tgtcagtatg gcctgttccc
     2461 gtactccgct gacctgtgcc acgctctctt ctccggcttt cacgctggcc gcaaaatctc
     2521 cggccagccg ggcatagcgg acattacggt ccggttcact gatgatggtg gccggtcgct
     2581 gttctccccc ctgccagcga tatgtgttca ccccggcatc cttcatggcc atcagtgcac
     2641 tgcctgtacc ggttcgctgc ccgctgtcgg ttatcaggac ctgtacgtta tgacgtgcgg
     2701 caccatccag cagggttaac gtctctttca gggagagttt ttcgccctgg tcgacgataa
     2761 cggtactgcc cggcgtgaag gccatgcctt ccagcaactg acgacgtccg gttatcagtt
     2821 caccggacag ccgttcatcc tgcttcaggt tcatctgcga gcgacggtca gcagcgataa
     2881 tctgcacctc ccgcccctgc tcccgggcca tcatgaccag ttcagccacc cgctcacgct
     2941 gcccggctgc accgccctgc ccggacacaa tggccagcga cggacggtcc tgtgccagaa
     3001 cactgacggc atcgctgtaa ccggccgtcc gggggacact tttctccgga tgtacggtca
     3061 cccggttctg tttcatgatg tcacgactga gtgcccggac tgacagctca tcgagcatat
     3121 gaatcccgga cgtgaacagc cccttctcac gatcgagggg gataagctgc tcacggctga
     3181 tggcctcatc gataccggcg cgtgcccgtt caatcacacc attttccggc ggcagtatgc
     3241 caaccgtcct ggccagcagg tccgtataca taaactgcac tttgcgttca cttaatccgg
     3301 caatggcctg tgtcaccgcc tgctgcacat ccggcccgtc ctgtgaagcg ggtccgggtg
     3361 tctgcgtgcg ggtatatgcc cgttgttccg ctgcgtcacg atatgcccgg atgtcgaacc
     3421 cggtttcctt cagcgtctgc atccactcgg ccattctgac ttcaggatcg acgtgctgtt
     3481 tggatttacg cgtatccagc gccgccacat cccgggattt cagcgaggcg tcttccccga
     3541 cggcctcccg gatagtctgt gagcgtccgg aaaaggcctc caccggtaca cccggcattt
     3601 cccacatacc gtgcttaccg accacttcag tttcatagcc cagcgcctca acctgctctt
     3661 tccgtttttc ccggtagagc ctgccaaagg caatctgatt agcgtacaca ttctcaatga
     3721 accccgtttt ccccactttg tcactgctca gtgtcttcca ttcgccgtta tgctgcgtaa
     3781 cattagtcac caccgcatgc gtgtgtaact gtggttcctg atcgcgactg gtgtcgtggt
     3841 taaacagtgc catcaccaga ttaccggtca gcaccgtttc tgactgtccg tccgtcatca
     3901 cccgtgtgga ggccagtgcc tccacctgac ggacagcaaa atccacggcc tggttatgtg
     3961 cttcaataag acgtttgtcc cccccgagca tggccatcat ggagacactt ttgggagcgg
     4021 agaaggtcag gtcgtagccg ggacgatgcc tgttactgcc atcctgcatg cggcttaaat
     4081 ccgccccgtc cggcagcctg ccctccagaa ggcgggtaaa gacatcctta tcgacactgc
     4141 cctgcagccc cagttgttca gccccctggc cggcccagcg ttctcccatg ctgcccagca
     4201 catagtaatt atccttgtcg gtataataat tatcggcact tccggccgat ctgacctgcg
     4261 cgatactcat cataaacgta tatccctttg acatatcccg gcatcaatcc cacaatagat
     4321 atacacaaga catatccaca taaggaggca aataatggaa accaccgtat ttctcagcaa
     4381 ccgcagccag gcggtcagac tgccaaaagc ggttgcattg ccggaaaacg taaaacgcgt
     4441 tgaagtgatt gctgtcggac gaaccagaat cattacgcca gccggagaga catgggacga
     4501 atggttcgac ggacacagcg tcagcgccga ttttatggat aacagggaac agcccggcat
     4561 gcaggaaagg gagtcattct gatgctgaag tttatgctcg ataccaacat ctgcattttt
     4621 acgataaaga acaaacccgc cagcgtcagg gagcgtttta acctgaacca ggggagaatg
     4681 tgtatcagtt cggtcaccct gatggagctg atatatggtg cagaaaaaag ccagatgcct
     4741 gaacgtaatc tcgctgtgat cgagggattt gtttcccgca ttgatgttct ggattacgac
     4801 gctgctgcag ccacacacac cggccagata agagcagaac ttgcccgtca gggacgccct
     4861 gtcgggccat ttgatcaaat gatcgcaggt catgcccgca gtcgggggct gattattgtg
     4921 actaataaca cccgggaatt tgaacgtgtg ggcggcctga gaactgaaga ctggagctga
     4981 cctgttcatc agaaatcatc tcccggctca acatcctccc cgcgttcccg gtgcacattg
     5041 atgttcacct cttcacgacg ctgcatctgc tgccaggtgt ccggatgatt ttcctgctgc
     5101 catgcctcat aagcggccat atccaccact tcaccggatt cactgatccc gggtggcagt
     5161 tgctgttcca tctcttcttc cggtttcatt ttcagctcct gctcaatccc ccctgccgga
     5221 acattcacac ctgaatctga cttcttatcg ttgatgacag gagacaccgg ctgttgcggc
     5281 tgttgcggct gttgcggctg ttgcggctgt tgcggctgtt gcggctgttg cggctgttgc
     5341 ggctgttcag cctgagtcac gtcttctccg gaaacaacct ccgggacatc cggttcgaag
     5401 aggctggcca tctgacgacc ttctgcttcc cttgcggcaa gtacggcact cagacggttc
     5461 tccatttccg ggttgatgtc acgcggaata aactccggag cgaccttcgg acgttcctga
     5521 tatttcagtg agagtttgac tgccggatac ggtccgggca gggtgacata gcaggtcaga
     5581 tccggcaggg actgaatgtc ggaataactg accagcgtct ggcgctccat atccttaccg
     5641 gtcgataccc cgtcacgtac cggatcagca ccgtaggaat actgttcgct ggctttcagg
     5701 tgctctttct caccaatttc accgacagcg aactccgcaa tcttatggct gggagaacgg
     5761 aaaaaggcac gggtgttcat gacgtcaaac agcgtggcag ccgctttctc accgtagata
     5821 tcttccagct gggcatagga ctggatacca aacacataac agccaccgaa cttacgggct
     5881 tccggcagga tctccaccag gtccggcagt ttgtgtaatg tgggtaactc atcacagaag
     5941 aaccacacac gacgattacg gttttctccc attgccagca gaccacgaat ggcaatggac
     6001 agccacatgg agatcaccgg cttcagggag gcatgagtgt cggcattcga cgagataaac
     6061 agccagccgt ttttctgatc ctcccggaca ccacgcatcc agtcacggat ggtgaagggc
     6121 tcaccgttat gctcaatccc ctgcaggtaa cggatggctt tcacatagtt ggtcagcaca
     6181 gcacggatgg aaatcgccgt tttctcaatt ttctcttcca ccaggttggc cgccggtgaa
     6241 ttacgcaggt aggtacgcag tttttcaatt ttgatggaaa gcagtgtgtc caccagtttg
     6301 ctgtagctgc ggttggggtc attacgcatc agatacgccg cttccgcaaa aatggtacgt
     6361 cctgaaccct gccagaacgg gtcctctttt gtgcccatcg ggatcagagt atttgcggta
     6421 ttatcaaaat ccggctgtgt caggcactcc ttccacaaat cccaggcggc acagcgtgca
     6481 tccagcggat tcaggatctt atcgatggag ggatcatagt aacttttaac aaattcccct
     6541 gaacggtcat aaatcaccac catatcacca cgctgacggg cgtagttggc cagacgacgg
     6601 ataacctccg acttaccggc cccgaccgtg ccgtgcagac agaagttctg gatttcggaa
     6661 tcccggataa tcggcagatc gccaatccgg atatcggagt ccctgccgtc ttttttcagc
     6721 atccgggcaa cgtctttcgg attgtctgtc agttgacgac cacctgtgac ttcattttcg
     6781 ctctgttgtt taccctgacg ccccagaatc caggagacaa caaagaaggt aatcaggcaa
     6841 ataaccagtg ccacaactgt ggccagaacg aatgcagacc ataactgctc accgcaccag
     6901 atcatatatt tatcatgcag tacctgagca gcgttcatcc ggaaggtttt gccgtaatac
     6961 tgaatctcat ataccggctg agacttgatt aaatcccgca tgccttccag cgtggtacac
     7021 caccagtaaa tacagccatt cacaaacgtc tgccagctta tttttaccca taaaaccaga
     7081 ccaacgagta tccagaaaaa aataaacagg cagtaaagca taatattggc gatctggctg
     7141 aacatgcgga tacgcggtaa tggctccaac ttattgatag tgttttatgt tcagataatg
     7201 cccgatgact ttgtcatgca gctccaccga ttttgagaac gacagcgact tccgtcccag
     7261 ccgtgccagg tgctgcctca gattcaggtt atgccgc
//
LOCUS       DAETQN010000183         7239 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.185, whole genome shotgun sequence.
ACCESSION   DAETQN010000183 DAETQN010000000
VERSION     DAETQN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.185"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..337
                     /locus_tag="K0W68_004036"
     CDS             <1..337
                     /locus_tag="K0W68_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBI3075456.1"
                     /translation="ADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGK
                     LGGVADTGGSGMVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARD
                     GGRLSGAYR"
     gene            337..666
                     /locus_tag="K0W68_004037"
     CDS             337..666
                     /locus_tag="K0W68_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3075457.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVKIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            676..1374
                     /locus_tag="K0W68_004038"
     CDS             676..1374
                     /locus_tag="K0W68_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBI3075458.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            1379..2122
                     /locus_tag="K0W68_004039"
     CDS             1379..2122
                     /locus_tag="K0W68_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3075459.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEVT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            2059..2661
                     /locus_tag="K0W68_004040"
     CDS             2059..2661
                     /locus_tag="K0W68_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBI3075460.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            2722..6201
                     /locus_tag="K0W68_004041"
     CDS             2722..6201
                     /locus_tag="K0W68_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBI3075461.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNSYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAIGRASDDAEGYLNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            6269..6868
                     /locus_tag="K0W68_004042"
     CDS             6269..6868
                     /locus_tag="K0W68_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBI3075462.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDGGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            6932..>7239
                     /locus_tag="K0W68_004043"
     CDS             6932..>7239
                     /locus_tag="K0W68_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBI3075463.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     "
BASE COUNT         1637 a         1727 c         2315 g         1560 t
ORIGIN      
        1 tgctgatttg agtcgctaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc
       61 aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgcgg
      121 tgctgatggt aagctggggg gtgtggcgga tactggtggt tcaggcatgg tgatgtttgc
      181 cccgcagtac aacattgaga tcaataacga tggcacgaac gggcagatag gtccggctgc
      241 cctgaaggtg gtttatgacc tcgggaaaaa agcggccgcg gactttatgc aacagcaggc
      301 ccgtgatggt ggccggttaa gtggagcata ccggtaatgg agacgtttca ctggaaagtg
      361 cgcccggata tgaatgtggt atcagagccg aaagtggtga cagtgaagct gggcgatggt
      421 tatgaacagc gtcgtgcggc gggactgaat aaccagttgt cgacttacag cgtgaagata
      481 cgtgttcgta aaggtgaaca cccattttta aaagcctttc tggaacggca cggtggcgtc
      541 cgcgcatttc agtggacgcc accttatgac tggaagccga tcagggtggt ttgtcgtaaa
      601 tggtcggcaa gcgtgggggc gttgtgggta accataacgg cagattttga acaggtcgtg
      661 gcataggagg ctctgatgca ggatattcca caggaaacac atcatgagac gacacgcctc
      721 actcagtcag cccaggtggt gctctgggaa atcgatctga cagaggtcgg tggtgaacgt
      781 tattttttct gtaatgagca gaacgaaaaa ggtgagccgg ttacctggca ggggcggcag
      841 tatcaggcat accccattca ggggacggga tttgaactga acggcaaggg cagtgctgcc
      901 cgtccgacac tgacggtttc taacctgcac ggaatggtca ccgggatggc ggaagacctg
      961 cagagtctgg tcggcggaac ggtggtcagg cgtaaggttt acgcccgttt tctggatgcg
     1021 gtgaacttcg tcaacggaaa cagcgacgcc gatccggaac aggaggtgat cagccgctgg
     1081 cgcatcgagc agtgcagcga actgagcgcg gtcagtgcct cttttgtgct ggccacacca
     1141 acggagacgg atggcgcggt tttcccgggg cgtatcatgc tggcgaatac ctgtatgtgg
     1201 acttaccgtt ctgatgagtg tggttacacg ggcagggcag tggctgacga gttcgacaaa
     1261 ccaacgacgg atatccggaa ggacaaatgc agcaagtgta tgcgcgggtg tgagttgcgc
     1321 aacaataccg gtaatttcgg cggtttcctt tccatcaata aactttctca gtaaatccat
     1381 gacacagaca gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg
     1441 tggcttcgtg gtaagaacgc cggagggaga caggtatctt cccagcgaga atatctccgg
     1501 tgagccggag gaacggttcc ggatggctcc ggaggactgg ctgcgggcac aaatgcaggg
     1561 tgagattgtg gcgctggtcc acagtcatcc cggtggtctg ccctggctga gtgaggctga
     1621 ccggcggctg caggtgcaga gtgatttgcc gtggtggctg gtctgccggg gggcgattca
     1681 caagttccgc tgtgtgccgc atctcaccgg gcggcgcttt gagcacgggg tgacggactg
     1741 ttacacgctg ttccgggatg cttatcatct ggcagggatt gagatgccgg attttcatcg
     1801 cggggatgac tggtggcgta acggtcagaa tctctatctt gacaatatgg aggtgacggg
     1861 tttttaccgt gtcgcactga cagaggcaca gccgggcgat gtgctgctgt gctgttttgg
     1921 ttcatcggtg ccgaatcatg ccgccattta ctgcggtgac ggcgggctgc tgcaccatat
     1981 tcctgaacaa ctgagcaaac gagagaggta caccgacaaa tggcagcgac gcacacactc
     2041 cctctggcgt caccgggcat ggcgcgcatc tgcctttacg gggatttaca acgatttggt
     2101 cgccgcatcg accttcgtgt gaaaacgggg gctgaagcca tccgggcact ggccacacag
     2161 ctcccggcgt ttcgtcagaa actgagtgac ggctggtatc aggtgcgcat tgccgggcgt
     2221 gatgcaggtg aaaccgaatt gtctgcccgt cttaatgagc cgctggcaaa tggtgcagtg
     2281 atccacatag taccgcgtct ggtgggagct aaaagtggcg gtgtgtttca ggcggtgctg
     2341 ggggcggctg ttatggcggt tgctatatgg atgccggggg taggaattat ggcgagtaat
     2401 ctgctgtttt ctctcggtgc cagtatgacg cttggcggtg ttgcacagat gctggcaccg
     2461 aaagccagaa ctccccgtac acagacaacg gataacggta agcagaacac gtatttctcg
     2521 tcactggata acatggttgc ccagggcaat gttctgcccg ttctgtacgg tgaaatgcgc
     2581 gtggggtcac gtgtggtttc tcaggagatc agcacggcag acgaagggga tggtggtcag
     2641 gttgtggtga ttggtcgctg atgcaaaatg ttttatgtga aaccgcctgc gggcggtttt
     2701 gtcgtttatg gagcgtgagg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa
     2761 gcgaaggaca acctgaaatc cacgcagttg ctgagtgtga ttgatgccat cagcgaaggg
     2821 ccggttgacg gtccggtgga tggattaaaa agcgtgctgc tgaacggtac gccggtcctg
     2881 gacagcgagg ggaagacaaa cttttccggt gttacggtgg tgttccgtgc aggtgagcag
     2941 gagcagacac cgccggaggg ttttgaatcc tccggctccg agacggtgct gggtacggaa
     3001 gtgaaatatg acacgccgat cacccggacc atcacgtcgg caaacatcga ccgtctgcgc
     3061 ctgaccttcg gtgtgcaggc actggtggaa accacctcaa agggtgaccg gaatccgtcg
     3121 gaagtccgcc tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc
     3181 accattaagg gcaaaaccac ttcgcagtat ctggcctcgg tggtggtggg taacctgccg
     3241 ccgcgcccgt ttaatatccg gatgcgcagg atgacaccgg acagcaccac agaccagctg
     3301 cagaacaaaa cgctctggtc gtcgtacacc gaaatcatcg atgtgaaaca gtgctacccg
     3361 aacacggcgc tggtcggcgt gcaggtggat tcagagcagt tcggtaacca gcaggtgagt
     3421 cgcaattatc atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg
     3481 cggcaataca gcggtatctg ggacggaacg cttaagccag catacagcaa caacatggcc
     3541 tggtgtctgt gggacatgct cactcatccg cgctacggca tggggaaacg tcttggtgcg
     3601 gcagatgtgg acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtgacg
     3661 gacggatttg gcggcacgga gccgcgcatc acctgtaact cttacctgac cacacagcgc
     3721 aaggcgtggg atgttctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac
     3781 ggacagatgc tgacgttcgt gcaggaccga ccgtcggata aggtgtggac ctataaccgc
     3841 agtaatgtgg tgatgccgga cgatggcgcg ccgttccgct acagcttcag cgccctgaag
     3901 gaccgccata atgccgttga ggtgaactgg attgacccag ataacggctg ggagacggcg
     3961 acagagcttg tggaggatac gcaggccatt gcccgttacg gtcgtaatgt cacgaagatg
     4021 gatgcctttg gctgtaccag ccgggggcag gcacaccgag ccgggctgtg gctgattaaa
     4081 acggaactgc tggaaacgca gaccgtggac ttcagcgtgg gcgcagaagg gcttcgccat
     4141 gtaccgggtg atgtcattga aatctgcgat gatgattatg ccggtatcag caccggtggt
     4201 cgcgtactgg cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg
     4261 ccgtcctccg gtaccacgct gataagcctg gttgacggaa gtggcaatcc ggtcagcgtg
     4321 gaggtccagt ccgtcaccga cggcgtgaag gtgaaagtga gccgggttcc tgacggcgtt
     4381 gcagaataca gcgtgtgggg gctgaagctg ccgacgctgc gccagcgtct gttccgctgc
     4441 gtgagtatcc gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg
     4501 gaaaaagagg ccatcgtgga taacggggca cactttgacg gtgaccggcg cggcacggtg
     4561 aatggtgtca cgccgccagc ggtgcagcat ctgaccgccg aagtcaccgc agacagcggg
     4621 gaatatcagg tgctggcgcg atgggacacg ccgaaggtgg tgaagggtgt gagcttcctg
     4681 cttcgcctga ccgtggcagc ggacgacggc agtgagcggc tggtcagcac agccaggacg
     4741 acggaaacca cataccgctt caggcagctg gcgctgggga attacagtct gacagtccgg
     4801 gcggtaaatg cccgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgcggca
     4861 ccggcagcgc ctgtcactat tgaactgata ccggggtatt ttcagataac ggcggtcccg
     4921 aaactggctg tatatgaccc gacggtgcag tttgagttct ggttttcgga aaagcggatt
     4981 gcggatatca ggcaggttga aaccagcgcc cgctatcttg gcacggcgct gtactggata
     5041 gccgccagta tcaatatcag gccgggccat gattattatt tttacgttcg cagtgtgaac
     5101 accgttggca aatcggcatt tgtggaggcc atcggtcggg cgagcgatga tgcagaaggt
     5161 tatctgaatt tttataaagg gttgatcaat aaaacgcatc tcggcaagga gttgtggacg
     5221 cagattgata acggtcagct tgcgccggac ctgactgaaa tcaggacgtc cattacgaat
     5281 gtcagcaatg aaatcacgca aaccgtcaat aaaaaactgg aaaatcagag tgctgcaatc
     5341 cagcagatac agaaagttca ggttgataca aataataacc tgaacagcat gtgggctgtg
     5401 aagctgcaac agatgaagga cggacgcctt tatattgcgg gtatcggtgc cggtattgag
     5461 aatacgccag caggaatgca gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt
     5521 aatcctgcga atggcaacac aaagccgatg tttgttggtc agggcgatca gatattcatg
     5581 aacgacgtgt tcctgaaatg cctgacggct ccgaccatta ccagcggcgg taatcctccg
     5641 gcattttccc tgacacctgg cggacggctg acggcgaaaa atgccgatat cagcggtaac
     5701 gtgaacgcga actccgggac gctcaacaac gtcacgatta ataagaactg tcgggttctg
     5761 ggaaaattgt ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaacctttc
     5821 ccacgggact cccgggcacc ggagaggtgg ccatcaggga ccattaccgt cagggtttat
     5881 gacgatcagc cgtttgatcg gcaaattgtt attcccgcgg tggcgtttcg cggtgctaaa
     5941 catgagcgga agaataacaa tatttattcg tcatgccgcc tgatagtgaa gaaaaacggt
     6001 gctgaaattt ataaccgaac gaccctggat aatacgctga tatatacggg tgttattgat
     6061 atgcctgccg gtcacggtca catgacgctg gagttttctg tatcggcatg gctggtaaat
     6121 ggctggtatc ccacagcaag tatcagcgat ttgctggttg tggtgatgaa gaaagccact
     6181 gcaggcatca cgattagctg aattttataa cccagatacg ggcaccagaa atggtgcctt
     6241 ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca ttttgtccgc
     6301 agccatctgt ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct
     6361 gagcgccggg tatcttcaga cccatactga tatgccaggc agcgatgacc tgaagggcat
     6421 taacgtgaaa taccgttatg agtttacgga cacgctgggg ctgattacgt cattcagtta
     6481 tgccaacgct gaagatgagc aaaaaacgca ttacagcgat acccgctggc atgaggattc
     6541 cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga atgaatggtt
     6601 cagcgcgtat gcgatggcgg gcgtggctta cagccgtgtg tcgaccttct ccggggatta
     6661 tctccgcgta actgacaaca aggggaaaac gcacgatgtg ctgaccggaa gtgatggcgg
     6721 tcgccacagc aacacgtctc tggcgtgggg ggctggcgtg cagtttaacc cgaccgaatc
     6781 cgtggccatt gaccttgctt atgaaggttc cggcagtggt gactggcgca ctgacggttt
     6841 catcgtgggt gtcggttata agttctgatt agccaggtaa cacagtgtta tgactgcccg
     6901 ctggttcagg cgggcttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa
     6961 agatggggcg ggtaaacctg tcgtaaattg tgcgattgaa ctgcgggcca gaagaaccag
     7021 tccgaccgtt gtggcacacg ttgttgccac ttgcgtgacg gacaataacg gtgcttatgt
     7081 gattgaggct gagccggggt attacgaggt tgcgcttcac tgtaacggct ggcagccaac
     7141 ccgtgtcggg gatattgatg tggcaccgac tgatgcaccg gggacactga acgcgtttct
     7201 gaatgcacca aaggatggtg atttacgtcc ggaggtgat
//
LOCUS       DAETQN010000184         6834 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.186, whole genome shotgun sequence.
ACCESSION   DAETQN010000184 DAETQN010000000
VERSION     DAETQN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.186"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1161)
                     /locus_tag="K0W68_004044"
     CDS             complement(<1..1161)
                     /locus_tag="K0W68_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBI3075464.1"
                     /translation="MLIYLYPVLLLFNILPVFFYGQMNSDLERFFGVPIGYIPDLIFY
                     FFVVLTSIITLRFHVSLWTKKLLFLGIIFLIYISIQMLLLSADISGVVILLSFFSNFI
                     ALVLLVSFCIGKDELYLTHSVRNINVVMCFGIICGVVKLFIGYSEDSNFIVYLNRNAT
                     AIIVVCFYCVYSYFYRGRKSWYVSSVLYSLFFLFLDSRAGIISFAISLFFVFLQLTKK
                     EKLLISLFFVPLLTLGISFTDIGTRLERMLSSSQVIFSGGNTLTKSQNDYRRVELVFI
                     GVDVLKENYLIGTGLGVANYVKAIDKKFLGSTNFGLAHNFYLSYSAQLGIIGFILLIS
                     VFYIMLSPIFKCGGYIGKGCVFALAFYVFFNEYILTPAIYIYISIFLSVVFIR"
     gene            complement(1230..2510)
                     /locus_tag="K0W68_004045"
     CDS             complement(1230..2510)
                     /locus_tag="K0W68_004045"
                     /inference="COORDINATES: protein motif:HMM:NF014051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBI3075465.1"
                     /translation="MFRKMIDAGGTFLLKAIFQIGVFVYFTHVSDITTFGIISYVFTV
                     YWFVLNFSDYGFRTKLVKDISDNSYSASELLSRSDGVKTYVFFFIFIIFMFYSYVSDS
                     ISLTLLVYISSAYFVCISSGRFSLLQAVGRFRCELYINIYSTIIYIGCNLFLSLFIEP
                     LYYSAISIFIYSISLLVFSSHKCNVPCFHIKRPSILVYKDFLDATPFAILVLLNVVLS
                     SIDLFILKEYFSYNSVAIYQVVTRVNTGLIIVFNVIYTVLLPSFSYYLKNSEWGNIRK
                     LQRYISLLVLLLCLCYYFFGIYFVGILFGDEYKVISSATFLIMFMALIKYNFWLINEL
                     YLVCSGNQSERVKSYCIGVVISMAVFFYFIPRYGWSGAVFGSAIATLVIGIFYIISVK
                     KDCGKILHDKYSLMMIFVPIFFYFIINGQQRLLY"
     gene            complement(2599..3624)
                     /gene="tviC"
                     /locus_tag="K0W68_004046"
     CDS             complement(2599..3624)
                     /gene="tviC"
                     /locus_tag="K0W68_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosaminuronic acid C-4 epimerase TviC"
                     /protein_id="HBI3075466.1"
                     /translation="MSRYEEITQQLIFSPKTWLITGVAGFIGSNLLEKLLKLNQVVIG
                     LDNFSTGHQYNLDEVKTLVSTEQWSRFCFIEGDIRDLTTCEQVMKGVDHVLHQAALGS
                     VPRSIVDPITTNATNITGFLNILHAAKNAQVQSFTYAASSSTYGDHPALPKVEENIGN
                     PLSPYAVTKYVNEIYAQVYARTYGFKTIGLRYFNVFGRRQDPNGAYAAVIPKWTAAML
                     KGDDVYINGDGETSRDFCYIDNVIQMNILSALAKDSAKDNIYNVAVGDRTTLNELSGY
                     IYDELNLIHHIDKLSIKYREFRSGDVRHSQADVTKAIDLLKYRPNIKIREGLRLSMPW
                     YVRFLKG"
     gene            complement(3717..4997)
                     /gene="tviB"
                     /locus_tag="K0W68_004047"
     CDS             complement(3717..4997)
                     /gene="tviB"
                     /locus_tag="K0W68_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HBI3075467.1"
                     /translation="MKFDTLNAKIGIIGLGYVGLPLAVEFGKKVTTIGFDINKSRIDE
                     LRNGHDSTLECSNLELLEATKLTYACSLDALKECNVFIVTVPTPIDKHKQPDLTPLIK
                     ASETLGKIIKKGDVIIYESTVYPGATEEDCIPVVEKVSGLKFNIDFFAGYSPERINPG
                     DKEHRVTNILKVTSGSTPDVAEYVDQLYKLIITVGTHKASSIKVAEAAKVIENTQRDV
                     NIALINELSIIFNKLGIDTLEVLEAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQS
                     VGYHPEMILAGRRLNDSMGQYVVSQLVKKMLKQRIQVEGANVLVMGLTFKENCPDLRN
                     TKVIDIISELKEYNINIDIIDPWCSTDEAQHEYGLTLCEDPKVNHYDAIIIAVAHNEF
                     REMGESAIRALGKDEHVLFDLKYVLDKKSIDMRL"
     gene            complement(5066..6172)
                     /locus_tag="K0W68_004048"
     CDS             complement(5066..6172)
                     /locus_tag="K0W68_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBI3075468.1"
                     /translation="MSKASEPQQTPYLIPQGVYPTYMPKAEDEIDLFELLGTLWKKKW
                     VILCVTLLTTGLAAVYAFTAKEQWTAKTYIQAPRIAELGSYLKFHQAYARILNQPLDT
                     NALANGLFSDLILIAESPDTKVKFLESTEYYKKETNNLSTEQDKKIWLAEQANKGLVI
                     TPPKEKGNTSYYIIQASADSAQEAYKLLQGYLKNVNNQAVTLSLDEFGQNVNTLLVNL
                     NKEIIDIDFQRKSEKLDQIAHIQRDLTTAEQAGIIDYRSSKGGFDNAQSSYKFLLGEK
                     LLSAELKATKDAPIIYPFRYYEVKRQIDELEGMLRDNIQAQAYRYQMKPSEPVIKDKP
                     NKALILILGALPGAMFAIVGTLVYATLKDKTKLD"
     gene            complement(6521..>6834)
                     /locus_tag="K0W68_004049"
     CDS             complement(6521..>6834)
                     /locus_tag="K0W68_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3075469.1"
                     /translation="SLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRVI
                     YQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFPGGKHGLAK
                     V"
BASE COUNT         2423 a         1342 c          997 g         2072 t
ORIGIN      
        1 acgtataaaa accaccgata aaaaaataga aatataaata tatatcgctg gcgtcaatat
       61 atactcatta aaaaagacat agaaagccaa agcaaaaacg catcctttac caatataccc
      121 tccgcattta aaaattggag acagcattat ataaaataca gaaataagca aaataaaacc
      181 aataatccct aactgagccg aataagataa ataaaaatta tgcgccaacc caaagttggt
      241 acttcctaaa aactttttat ctatagcctt tacataattt gcaacaccta atccagtgcc
      301 aattaaataa ttttctttta aaacatcaac cccaataaat actaactcaa ctcgacgata
      361 atcattctga ctttttgtaa gagtgttacc accagagaat ataacctgtg acgaagacag
      421 cattcgttca agacgagtgc ctatatcagt aaaagaaata cctaaagtta gaagaggaac
      481 aaaaaacaat gatattaata acttttcctt ctttgttaac tgaagaaaaa caaaaaacaa
      541 cgatatagca aatgatatta ttcctgctcg gctatccaga aaaagaaaga acagagagta
      601 caatacagat gagacatacc aagactttcg accacgataa aagtatgaat atacacaata
      661 aaagcacact actataattg cggtggcatt tctatttaaa taaactataa aattactatc
      721 ttcagaataa ccaataaata attttacaac tccacagata ataccaaaac acattacaac
      781 atttatattt ctaaccgaat gagttaaata aagctcatct ttaccaatgc aaaatgacac
      841 caaaagaacc aaagctataa aattagaaaa aaacgataat aaaattacga cacctgatat
      901 atccgctgat aacaacaaca tctgaatgct gatataaatc aggaatatga tgcctaaaaa
      961 taataatttc tttgtccaca gagaaacgtg aaacctcaac gttattatag atgttaaaac
     1021 aacaaagaaa taaaatatta gatctggaat atagccaata ggaactccaa aaaaacgctc
     1081 taaatcagag ttcatttgtc cataaaaaaa aaccggaagg atattaaata acaaaagtac
     1141 aggatatagg tatattaaca tacttaacct ttattaggct tccaatcgag tctaaacata
     1201 ttaatggaac gatataaaac cacaacatat taatataaca accgctgctg accattaata
     1261 ataaaataaa agaaaattgg gacaaagatc atcattagtg aatacttatc atgaagaatt
     1321 ttcccacaat cttttttcac agaaataata taaaatattc caattactaa tgttgcaatg
     1381 gcacttccaa aaaccgcccc actccatcca taccgaggta taaaataaaa gaaaaccgcc
     1441 attgaaatga ccacaccaat acaatacgat ttaactcgct cgctttgatt tccactacac
     1501 acaagataaa gttcatttat tagccaaaaa ttatatttaa taagagccat aaacattatc
     1561 aaaaatgttg cagaagatat taccttatac tcatcaccaa acaatatccc tacgaaatag
     1621 atgccaaaaa aataatagca taaacatagt aataagacta acagtgatat atatcgttgt
     1681 aatttcctta tattacccca ttcagaattt ttcagataat aagaaaatga aggcaataaa
     1741 acagtataaa taacattaaa cactattatt agaccggtat taaccctagt taccacctga
     1801 tatatagcaa cactattata agagaaatat tcttttaata taaaaaggtc aatactagat
     1861 aaaacaacat ttagtaacac cagaatagcg aacggagttg catccaaaaa atctttataa
     1921 actaaaatac ttggtctttt tatatgaaaa catggcacat tgcatttatg tgatgagaaa
     1981 accaaaagcg aaattgagta tatgaatatt gatatcgcac tataatatag aggttcgata
     2041 aacagagata aaaataaatt acacccaata tatataattg ttgagtagat atttatatat
     2101 aattcacatc taaaccgacc aacagcctgt agcaagctaa atctaccact tgaaatacaa
     2161 acaaaatatg cagatgaaat ataaacaagc agagttaatg aaattgaatc agaaacataa
     2221 gaataaaaca tgaagattat aaaaatgaag aaaaaaacat atgttttaac tccatcactt
     2281 cttgataata attctgatgc agaataacta ttatcagaaa tatctttcac taattttgtt
     2341 ctaaatccat aatcagagaa gttaagcaca aaccaataaa cagtaaacac ataactaata
     2401 ataccaaatg tagtaatatc tgacacatgt gtgaaataaa caaaaactcc tatttgaaat
     2461 attgctttaa gtaaaaatgt accacctgca tcaatcattt ttctaaacat acactgacca
     2521 tttagaataa atacatggat tttcacccct gcattaataa cagaggtgaa atagattatt
     2581 tattcatgtt aatataattt agccttttaa aaatctcaca taccacggca ttgaaagtcg
     2641 taatccctct ctgattttta tatttggtct atactttagt aaatctatag ccttagtaac
     2701 atcagcctga gaatgcctaa catctccaga tctaaactct ctatacttaa tgctcaattt
     2761 atcgatatgg tgaattaaat taagctcatc ataaatgtaa ccagataatt catttaacgt
     2821 tgttctatca ccaactgcaa cattatatat attatcttta gcactgtcct tcgctaatgc
     2881 agataatata ttcatttgta taacattatc tatataacaa aaatcacgac tcgtttcacc
     2941 atcgccatta atatatacgt catcaccttt aagcattgct gctgtccatt ttggaattac
     3001 tgcagcataa gctccattag gatcttgacg acgaccaaat acattaaaat aacgtaatcc
     3061 aatagtttta aaaccatatg ttcgagcata tacctgagca taaatctcgt taacatattt
     3121 agtaactgca taaggagaaa gtggattacc aatgttttcc tctacttttg gtagtgcggg
     3181 atgatctcca taagttgagc ttgatgcagc ataagtaaaa ctttgtactt gtgcattttt
     3241 agccgcatgt aagatattca aaaatccagt aatattagtt gcattggttg ttataggatc
     3301 aacaattgaa cgaggtacag aacctagcgc agcctgatgt aagacatgat caacaccttt
     3361 cataacttgc tcacaggtag tgagatctcg aatatcacct tctataaagc aaaatcgact
     3421 ccactgttca gtggaaacta atgttttaac ttcatcaaga ttatattgat gtcccgtgga
     3481 aaagttatct aacccaataa caacctggtt taatttaagt aacttttcta aaagatttga
     3541 tcctataaag ccagcgacac cagtaattaa ccaagttttc ggtgaaaaaa ttaactgctg
     3601 tgtaatctct tcatagcgag acatataaat atccatgtat attaatcaag tgctaaacct
     3661 agcataaaat gctgaggtgt atcatatcaa agaggatttg atttttttaa tcactcttac
     3721 aagcgcatat cgatactttt tttatcaagc acatatttta aatcgaacaa aacgtgctcg
     3781 tctttaccta atgcacgaat agcgctctct cccatctcgc gaaactcatt gtgtgcaaca
     3841 gcgataatta ttgcatcata atgattaact ttaggatctt cacataaagt taatccatat
     3901 tcatgttgtg cctcatcggt agaacaccat ggatctataa tatctatatt gatattgtat
     3961 tcttttaact ctgaaataat atcaatcact ttagtgtttc gtagatctgg gcaattctct
     4021 ttaaatgtaa gccccatcac taacacattc gccccttcaa cttgaatccg ttgtttcaac
     4081 atttttttga ctaactggga aacgacatac tgccccatac tatcatttaa acgacgtccg
     4141 gctaaaatca tctccggatg atagccgaca ctttgcgctt tatgtgtaag ataataagga
     4201 tctacaccta tacagtggcc acctactaaa ccgggcctaa aaggtaaaaa attccacttc
     4261 gtacctgcag cctcaagaac ctctaaggta tcaatcccta acttattaaa tataatagat
     4321 aactcattaa tcaatgcaat attgacatct cgctgcgtgt tttcaattac ttttgcagcc
     4381 tctgctactt ttatcgatga tgctttatgc gtaccgacag taattattaa tttatatagc
     4441 tgatctacat actcagcaac atccggtgta gatccactgg tcaccttaag gatattagtt
     4501 acacgatgct ctttatcccc aggattaata cgctcaggtg aataaccggc aaaaaaatca
     4561 atattaaact taagacctga tactttctct acaactggta tacaatcttc ttctgtcgct
     4621 ccagggtaaa ctgttgactc ataaataata acatcgcctt tctttattat cttacccaat
     4681 gtttcagatg ctttaattag aggtgttaga tctggctgtt tatgtttatc aattggagtt
     4741 ggaacagtta caataaatac attacactct tttagtgcat ctaatgaaca ggcgtacgtc
     4801 aatttagttg cttctaacaa ctctaaattt gagcactcta atgtactatc gtgaccattt
     4861 cgtaattcat caatacgaga cttattaata tcaaatccaa tcgtcgttac tttctttcca
     4921 aactcaacag caagaggcaa tccaacataa ccaaggccta taatcccaat tttcgcattc
     4981 aaagtatcga atttcatttt cttatccatc ataaagtgca catagaacat ctatttcgca
     5041 ttgacacaac aatacgtaac ccagtttaat ctaacttggt tttatctttt aatgtcgcat
     5101 aaactaatgt accaactata gcaaacattg cccctggtaa tgcaccaaga atcaaaatta
     5161 atgctttgtt gggtttgtct tttataactg gctcagatgg cttcatttga tatcgatatg
     5221 cttgcgcctg aatgttatcg cgtaacattc cttctaactc atcaatttga cgtttcactt
     5281 cgtaatatct aaatgggtaa ataattggcg catctttagt tgcttttagc tctgctgata
     5341 acagtttttc gccgagcaag aacttatagc tactttgcgc attatcgaag ccgcctttgc
     5401 tagagcgata atcaatgatt ccggcttgtt ccgcagttgt taaatctcgc tgaatatgag
     5461 ctatttgatc aagcttttct gattttctct ggaaatctat gtcaataatt tctttattta
     5521 gattaaccaa aagagtatta acattttgac caaactcatc aagacttaat gttacagctt
     5581 gattattaac attctttaga tatccctgca gtagtttata tgcctcttgc gctgagtctg
     5641 ccgatgcttg tattatgtag taacttgtat ttcccttttc ctttggtggc gtaatcacaa
     5701 gacctttatt cgcttgctca gctaaccaaa ttttcttatc ttgttcagta gataaattat
     5761 ttgtttcctt tttataatac tcagtactct ctagaaattt aactttggtg tctggcgatt
     5821 cagcaatcaa aatcaaatcg gaaaacaatc cattagccaa cgcattcgta tctaacggtt
     5881 gatttaatat tcgggcatac gcttggtgaa atttaagata gctgcctaat tcagcaatac
     5941 gtggtgcttg aatataggtt ttagccgtcc actgctcttt tgcggtaaag gcatacactg
     6001 ctgctaaccc cgtagtcagc aacgtgacac ataaaataac ccattttttc ttccacaagg
     6061 tgcctaaaag ctcgaaaaga tcgatttcat cctctgcttt tggcatataa gttggataga
     6121 ccccttgcgg gatcagataa ggggtctgtt gtggttcaga tgcttttgac atgaaaccaa
     6181 tccattattt atcaatgggt taaaaataaa aatgaagaat aagcctgaca aaaaagtacg
     6241 ctaccgccct tgagtttcag ctcccaaagc gctgcgcaga aacaggatat agccaactgc
     6301 gatacaacaa gtgatatctg ggattagagg acataatcga gaacactccg tgtgaagaga
     6361 catatacagt cccacacttc cctgctgcaa accggcggat agcccgcaga atttggagtg
     6421 cagattataa agcaatgcct gtataagacc aaatctgcct tcaaaatgat aacagtatgt
     6481 ttgtaaattc tgtgaggcta aatagctgcg cttaataccg ctacactttt gccagcccat
     6541 gtttgcctcc ggggaatggg ctgacggttt ccataaaatg gcgaactttt ttcaacagtt
     6601 gccacattga gctgcactga tgattacgcg ttattgtgtc gtgaagtgcc tgccatagcc
     6661 gttcaacatg gttcacccat ggcgagtaaa ccggctgata aatgaccctg aacttcgggt
     6721 tctccttcag ccagctctgt gtttcccggc ttttgtggat aatgtagttg tccacgatca
     6781 gcgtgatggt tttcgcccga cggtatgtcg ctttaagccg cttcagcagg ctga
//
LOCUS       DAETQN010000185         6802 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.187, whole genome shotgun sequence.
ACCESSION   DAETQN010000185 DAETQN010000000
VERSION     DAETQN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.187"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1253
                     /locus_tag="K0W68_004050"
     CDS             <1..1253
                     /locus_tag="K0W68_004050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075470.1"
                     /translation="ENMLLEYVERKISLALSKYPKVRDVIKFFYLYIASLFGIILNKN
                     KTVIQSKIYEISIDDSEESFFGYYDHSPMSSNGRYVLFHSSAFSTKRHPKKVKYISIC
                     VKDLLNNKVYKLYDTRAFNWQQGSRLMWIDDDNIIFNDYENNGYISVVYSLSLMKVIK
                     KINYPIYDVNNYKAVTLDFSWLAKYDSDYGYYNKKSFSTDISIINLNTGGIELFLSLD
                     EMLKRTNFKCNIDVEHVVNHFMFAPDGRSVMFIHRYYTPKGKRERLIHWNLINDNVRV
                     LINESIISHCCWNGNDEIIGFFGAEIDSLNYYRLSIESCNTEKLFFDARKYSDGHPTI
                     VHNRYIISDTYPDKNRIKKLFVYDLVKNDYRELGLFYESMSFFSYSRCDLHPRISVDN
                     RFLFVDSVHSGKRKLYFMRSGICE"
     gene            1246..2232
                     /locus_tag="K0W68_004051"
     CDS             1246..2232
                     /locus_tag="K0W68_004051"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBI3075471.1"
                     /translation="MSDVLVSLIIVCFNAEKYIEKSLLAFINQDVGLDKFELIIVDGD
                     SSDNTISIVQDVFSKHSNIKHKIINNKKRTLATGWNIGVLEANGKFVCRVDAHSDIPN
                     NYISKLLDDYFNIMQFDDSVVGVGGVLTNSYKTKFGSIVADFYASKFGVGNSPFRCVD
                     KNNRLKKTDTAVFALYNKDVFFDVGLFNEVLDRNQDIDFHKRVLSNNLSLYTDNSLFV
                     EYYVRDNFKDFIKKGFLDGFWVVMSGAYYFRHIVPLFFVLYLIVSFSLFFATGDYIYL
                     SFLFFYFLISILFSIRDGRSFIGRVFLPFIFLSYHISYGCGSLLSFLKRYFK"
     gene            2229..3383
                     /locus_tag="K0W68_004052"
     CDS             2229..3383
                     /locus_tag="K0W68_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBI3075472.1"
                     /translation="MKNFIPFALPEIGEEEIAEVIDSLRSGWITTGPKAKQFEQEFSN
                     YLGANVQSLAVNSATSGLHLALEAVGVKPGDQVIVPSYTFTATAEIVRYLGADPVIVD
                     VDRKTFNISVDAIEKAITNETKAIIPVHFAGLACDMDSILSIAKKYDLKVVEDAAHAF
                     PTTYKGSKIGTLDSDATVFSFYANKTMTTGEGGMVVSKNKDIIERCKVMRLHGISRDA
                     FDRYQSKTPSWFYEVVAPGFKYNMPDICAAIGIHQLRKIDDFQKKRQRMAKIYDDALK
                     ELPLELPEWPTNASDIHAWHLYPIRLKTDSAINRDDFIKKLSDLGIGCSVHFIPLHKQ
                     PVWRDTYNLNASDFPVSEECYLNEISIPLYTKMTDQDQLFVIKSIRQLFM"
     gene            3400..3990
                     /locus_tag="K0W68_004053"
     CDS             3400..3990
                     /locus_tag="K0W68_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBI3075473.1"
                     /translation="MKRIFDVIVAGLGLLFLFPVFIIVSMLIVADSKGGVFFRQYRVG
                     RFGKDFRIHKFRTMFIDSEKKGRITVGQDARVTRVGWYLRKYKIDELPQLIDVLSGTM
                     SLVGPRPEVREFIDEYPDDIREKVLSVRPGITDLASIEMVDENEILSSYDDPRRAYID
                     IILPIKQRYYLDYVANNSVKYDCVIIWKTIIKILSR"
     gene            4146..5924
                     /locus_tag="K0W68_004054"
     CDS             4146..5924
                     /locus_tag="K0W68_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBI3075474.1"
                     /translation="MWFAAAILTIFTVFIFIRIGLYRAVLRYVSAKIMLLIPVGILAS
                     TLSLVVISYSLSIMLPRTVVGIYFLVLLLLTSGSRLLFRMILNYGVKGSAPVLIYGAG
                     ESGRQLLPALMQAKEYFPVAFVDDNPRLHKAVIHGVTVYPSDKLSYLVDRYGIKKILL
                     AMPSVSKSQRQKVITRLEHLPCEVLSIPGMVDLVEGRAQISNLKKVSIDDLLGRDPVA
                     PDAKLMAENITGKAVMVTGAGGSIGSELCRQIVRYKPAKLVLFELSEYALYAIEKELS
                     ALCDKEVLNVPVIPLLGSVQRQNRLQMVMKSFGIQTVYHAAAYKHVPLVEHNVVEGVR
                     NNVFGTLYCAESAIESGVETFVLISTDKAVRPTNTMGTTKRLAELVLQALSARQSQTR
                     FCMVRFGNVLGSSGSVVPLFEKQIAQGGPVTLTHRDIIRYFMTIPEASQLVIQAGAMG
                     HGGDVFVLDMGDPVKIYDLAKRMIRLSGLSVRDDKNPDGDIAIEVTGLRPGEKLYEEL
                     LIGDSVQGTSHPRIMTANEVMLPWQDLSLLLKELDQACHDFDHERIRSLLLQAPAAFN
                     PTDDICDLVWQQKKSLLSQASNVIRL"
     gene            6043..6550
                     /gene="aqpZ"
                     /locus_tag="K0W68_004055"
                     /pseudo
     CDS             6043..6550
                     /gene="aqpZ"
                     /locus_tag="K0W68_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
BASE COUNT         1914 a         1084 c         1498 g         2306 t
ORIGIN      
        1 atgagaatat gttgttagaa tatgttgaaa gaaaaatttc cttagccttg agtaagtatc
       61 ctaaggtaag ggatgttatt aagttctttt atttatatat cgcatcatta ttcggaatta
      121 ttttgaataa aaataagacg gttattcaat caaaaatata cgagatttca attgatgatt
      181 ctgaagaatc attttttggc tattatgacc atagtccaat gagctctaat gggcggtacg
      241 tattgttcca ctctagtgcg tttagcacta aacgacatcc aaagaaagtt aagtatatat
      301 ctatttgcgt aaaagacctt cttaataaca aagtttataa gctatatgat acgcgagcat
      361 ttaattggca gcagggaagc cgattaatgt ggattgatga tgacaatata atttttaatg
      421 actatgaaaa taatggatac attagtgttg tctattcttt gtctttgatg aaggttataa
      481 aaaaaataaa ctatccgatt tatgatgtga ataattacaa ggctgtgacg ttagatttct
      541 catggctggc taaatatgat agcgattatg gttattataa taaaaaatca ttttctacag
      601 atatttcaat cattaatttg aatacggggg gaatagaatt atttttatcc ttagacgaaa
      661 tgctaaagag aactaatttt aaatgtaata ttgatgttga acatgtggtc aatcatttta
      721 tgtttgctcc cgatggacgt tccgttatgt tcatacatcg atactataca cctaaaggaa
      781 agcgtgaaag gttaatacat tggaatttaa taaatgataa tgttcgagtc ctaataaatg
      841 aatcgattat tagtcattgt tgttggaatg ggaatgatga aattataggt ttttttggtg
      901 cagaaataga ttcgctaaat tattatagat tgtcaattga atcctgtaat acagagaaat
      961 tgttttttga tgcaagaaaa tattctgatg gacatcctac tatagttcat aatagatata
     1021 ttatatctga tacttaccca gataaaaata gaattaaaaa gttgtttgtt tatgaccttg
     1081 tcaaaaatga ttatcgcgag cttggattat tttatgagtc aatgagtttt ttttcttatt
     1141 ctcgatgtga cttacatcca aggatctcgg ttgataatag atttttgttt gttgattcag
     1201 ttcactcagg gaaaagaaaa ctatatttta tgaggagtgg tatttgtgag tgatgttcta
     1261 gtatctttaa ttatagtttg ctttaatgca gagaagtata ttgaaaaatc tcttttggca
     1321 tttattaatc aagatgttgg attagataaa tttgaattga ttattgtaga tggggattca
     1381 tctgataata caatatctat tgttcaggat gttttttcta aacatagcaa cattaagcat
     1441 aaaattatca ataataaaaa aagaactctt gctacgggtt ggaatattgg ggtgctagaa
     1501 gctaatggta agtttgtgtg tagagttgat gcacatagtg atattccaaa taactatata
     1561 tctaaattat tagatgatta ttttaatatt atgcagtttg atgatagcgt tgttggtgtt
     1621 ggaggtgtat taactaattc ttataaaact aagtttggtt caattgtagc ggatttttat
     1681 gcatcgaaat ttggtgttgg taattctcca tttaggtgcg tagacaaaaa taatcgacta
     1741 aaaaaaacag atacagctgt ctttgcttta tataataaag atgtgttttt tgatgttgga
     1801 ctttttaatg aagtattaga tagaaatcaa gatattgatt ttcataagag agttttaagc
     1861 aataatttgt cattatatac agataatagt ttatttgttg agtattatgt tagagataat
     1921 tttaaagatt tcataaagaa aggttttctt gatggttttt gggttgttat gtctggagca
     1981 tattatttta gacatatagt gccacttttt tttgttttgt atttaattgt atctttttct
     2041 cttttctttg ctactggtga ttatatatat ttatcttttt tattttttta ttttcttatt
     2101 tctattttgt tttcaattcg agatgggcga agttttatag gtagagtatt tcttcctttt
     2161 atatttttgt cttatcatat ttcttatgga tgtggatcgt tattatcttt tttgaaaagg
     2221 tattttaaat gaaaaatttt attccttttg cgttacctga aattggcgaa gaagaaattg
     2281 cagaggtaat tgactcttta cgttcaggtt ggattacgac aggtcctaag gctaagcaat
     2341 ttgaacaaga attttctaat tacctaggag cgaacgttca atcattagct gttaactctg
     2401 ctacgtcggg cttacatttg gctcttgaag ctgttggcgt aaagccggga gaccaagtta
     2461 ttgtcccatc atatacattc actgctactg ccgaaattgt caggtacctt ggtgctgatc
     2521 ctgtaattgt tgatgtagat cgtaaaacat ttaatatatc agttgatgcc attgagaagg
     2581 ctattactaa tgaaacaaag gcgattattc cagtacactt cgctggatta gcttgtgaca
     2641 tggattcaat cttatcaatt gctaaaaaat atgacctaaa ggttgtcgag gatgccgctc
     2701 atgcatttcc tacaacatat aaaggaagta agataggaac gcttgattca gatgctacgg
     2761 tttttagctt ctacgccaat aaaactatga caaccggtga aggcggaatg gttgtttcaa
     2821 aaaataaaga tataattgag cgttgtaagg taatgcgttt acatggaatc agtcgtgacg
     2881 cttttgaccg gtaccagtct aaaactcctt cttggtttta tgaggttgta gctccagggt
     2941 ttaaatacaa tatgcctgat atctgtgcgg caatcggtat tcatcaactt agaaagatcg
     3001 atgattttca gaaaaaacgt caacgaatgg caaaaattta cgatgatgcg ttaaaagaat
     3061 tgccacttga attgcctgaa tggcctacta atgctagtga tattcatgct tggcatctat
     3121 atcctatccg cttaaaaact gattcggcta ttaatcgcga tgattttatt aagaagttat
     3181 cagatcttgg aattggttgt tctgtccatt ttataccgtt gcataagcaa ccggtttggc
     3241 gtgatacata taatttgaac gccagtgact ttccagtttc tgaggagtgt tatttaaatg
     3301 aaatatctat tcctctttat actaaaatga cggatcaaga tcagttgttc gttatcaaat
     3361 cgattagaca attatttatg taatggtatt ttatattaaa tgaaacgtat ttttgatgtt
     3421 atcgtggcag gcttaggcct gctttttcta tttcctgttt ttatcattgt gtcaatgtta
     3481 attgttgctg attctaaagg gggggttttt tttaggcagt atagagttgg gagatttggg
     3541 aaagatttta ggatacataa atttagaacg atgtttatcg attcagaaaa aaaaggacgg
     3601 ataacagttg gtcaagatgc tcgggtaacc agagttggat ggtatttacg gaagtacaaa
     3661 atcgatgagc ttcctcaatt gatagatgtt ctttctggaa caatgagttt ggttggccca
     3721 agaccggaag tgagggagtt tattgatgag tatcctgatg atataaggga aaaagtttta
     3781 tcggttaggc cagggataac tgacttagca tctatagaaa tggtagatga aaatgagatt
     3841 ttgtctagtt atgatgaccc acgtagggct tatatagata taattcttcc aatcaagcaa
     3901 agatattatt tagattatgt tgctaacaat tcagtaaagt atgattgtgt gataatttgg
     3961 aaaactatta ttaagatttt gtcgcgataa taaggtagtg taggatgatt gatagaatat
     4021 tggagctgcc aagaattgtt aagagaggta tcatcatctg cattgatgta gttatggtga
     4081 tattctcatt ttggttgtct tattggttga ggcttgatga gcaaacggct tttcttagtg
     4141 caccgatgtg gtttgctgca gctattctta ccatatttac cgtgtttata tttatcagga
     4201 ttgggcttta tcgggcagtc ttacggtatg ttagtgcaaa gataatgttg ctaataccag
     4261 ttggtattct ggcctcaacg ttatctcttg tcgttatatc atattcgcta tccataatgt
     4321 tgccgcgcac tgttgtcgga atttattttt tggttttact tttactgaca tcaggctcta
     4381 gattgctttt tagaatgata cttaactatg gagttaaggg tagtgcgcct gttttgattt
     4441 atggcgctgg tgaatctggc cgacaattat tgccagcatt aatgcaggca aaagaatatt
     4501 ttcctgtggc atttgtggat gataatcctc gcttgcataa ggctgtcatt catggtgtaa
     4561 cagtttatcc ctcggataaa ctgagttacc ttgtagatcg ctatggtata aagaaaattc
     4621 ttttggcgat gccgagcgtc agtaagtcac aaaggcagaa agtgattact cgtttagagc
     4681 atctaccgtg tgaagttctc tctattccgg gtatggtcga tttagtcgaa ggtcgagcac
     4741 aaatcagtaa tctaaaaaaa gtatcgattg atgacttact aggtcgtgat ccggttgctc
     4801 ctgatgccaa attgatggcc gaaaacatta ctggcaaagc cgttatggtc actggggcgg
     4861 gaggctcgat cggctctgag ctttgtcgtc aaattgttcg atataagccg gccaaattgg
     4921 ttctatttga actgtctgaa tatgccctct acgctattga gaaagagctc tcggcgctgt
     4981 gtgacaaaga agttttgaat gttccagtga tccctctgtt gggctcggtg cagcgtcaga
     5041 atcgcttaca gatggtgatg aagtcctttg gtattcaaac ggtttatcat gcggccgctt
     5101 ataaacatgt gcctctggtt gagcataatg tggtggaagg ggtacgtaat aacgtgtttg
     5161 gtaccttgta ctgcgctgag tcagcgatcg aaagtggcgt tgaaactttt gtgttgattt
     5221 ccaccgataa agcggtgcgc ccgaccaaca ctatggggac aactaagcgt ctggccgaat
     5281 tggtattgca ggctttgtct gcacggcaaa gccaaactcg cttttgtatg gtgcgatttg
     5341 gtaatgtact cggttcttcg ggctctgtcg tgccgttgtt tgaaaaacag attgcccaag
     5401 gtgggccagt taccttgact catcgtgaca ttattcgcta tttcatgaca attccggaag
     5461 catcacagtt ggtgattcaa gcgggggcga tggggcatgg cggcgatgtc tttgtcttag
     5521 acatgggcga tccggtcaag atttatgact tagccaaacg catgatccgg ttaagtggct
     5581 tgagtgtacg ggatgataaa aatccagatg gcgatattgc cattgaagtt acgggattac
     5641 gtccaggtga gaaactgtat gaagaattac tgattggtga ttcagttcaa ggtacctctc
     5701 atccacgaat tatgacggcc aacgaagtga tgctaccgtg gcaggatcta tcgctcttac
     5761 ttaaagagct ggatcaagct tgtcatgact ttgatcatga gcgaattcgc agtttgttgt
     5821 tacaagcacc agcggcattc aatccaactg atgatatttg cgatctagtt tggcagcaga
     5881 aaaaatcgct gttatcacaa gcgagcaatg tcattcgcct gtgattgctt aggtttaacc
     5941 ttccacacca attcttcacc tctcttacaa atccccgcta ggcggtacat cgtgaccgcc
     6001 tttaccctga tgcctgctct ttaacaaaca ggacatcagt gtatgtttaa accttttagc
     6061 gccgaatttt tcggcacttt ctggctggtt ctgggtggct gtggtagcgc cttgatctct
     6121 gctgctttcc cacagttagg tataggcttt ttgggcgtgg cgttggcgtt tggtctgaca
     6181 gtagtcacca tggcttatgc ggtcgggcac atctctggtg cgcattttaa ccccgcggtg
     6241 accttgggtc tgtgggccgg tggacgcttc ccagcagcgc gcgtgttacc ttacattatc
     6301 gctcaggtta tcggcggtat tgccgctgcg gcagtgctgt atggtatcgc cagcggtaag
     6361 gctgggtttg atgcgacaac cagcggtttt gcggctaatg gttatggcct ccattcacct
     6421 ggcggctatg cgttaagcgc ctgtatgctg agcgagtttg tcctcagtgc gttttttgtc
     6481 cggagcgaca gaaaaacgcg ctcctgcggg ctttgcgcca ctggcgattg gtctggtaat
     6541 caccccgtaa attaaccagc gtcaaaagta gaattttctc gtaccataaa cgcaggagat
     6601 tctttatgaa aacatcaaaa tttaccgaca gccaaatcat ggcgatcctc aaatgaaccg
     6661 ccccgggaat cctggagact aaacttcctg agaaagaggt aaacaggatg actaaaaata
     6721 ctcgtttttc ccccgaagtc cgtcaacggg cagtccgtat ggttctggaa agtcagggcg
     6781 aatatgactc acaatgggcg ac
//
LOCUS       DAETQN010000186         6647 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.188, whole genome shotgun sequence.
ACCESSION   DAETQN010000186 DAETQN010000000
VERSION     DAETQN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.188"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..737)
                     /locus_tag="K0W68_004056"
     CDS             complement(<1..737)
                     /locus_tag="K0W68_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HBI3075475.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="K0W68_004057"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="K0W68_004057"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBI3075476.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="K0W68_004058"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="K0W68_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBI3075477.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="K0W68_004059"
     CDS             4397..4678
                     /locus_tag="K0W68_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBI3075478.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4921..5463
                     /locus_tag="K0W68_004060"
     CDS             4921..5463
                     /locus_tag="K0W68_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3075479.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5543..6217
                     /locus_tag="K0W68_004061"
     CDS             5543..6217
                     /locus_tag="K0W68_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBI3075480.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            6233..>6647
                     /locus_tag="K0W68_004062"
     CDS             6233..>6647
                     /locus_tag="K0W68_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI3075481.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT         1755 a         1507 c         1594 g         1791 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg atttttattg atatcaacaa taccatttac ataaatctac attaattgat
     4741 agatgcgata aatagttaat actcttaaat atttgttgta tttatattgc aatggttaat
     4801 ttgtttcaaa taaataaaac aatgtgttta cattgcggta acttttttga tgtgagaaat
     4861 ttgtttccaa gagatacaga ctcttaacaa ccaaaaaaaa attttattaa ggaacatatt
     4921 atgaaacgtt caattattgc tgccgctgtc ttttcttctt tttttatgag cgctggagta
     4981 tttgctgcag acgttgatac cggaacatta actattaagg ggaatattgc agaatctccg
     5041 tgtaaattcg aagcgggtgg tgattcagta agtattaata tgccgactgt accaaccact
     5101 gtctttgaag gtaaagctaa atattctacc tatgatgatg cagtcggtgt aaccagcagc
     5161 atgttaaaaa ttagctgccc gaaagaagtt gctggtgtaa aactctcgtt gattaccaac
     5221 gataaaataa ccggtaacga taaggcgata gccagtagca acgataccgt gggttactat
     5281 ctctatttag gtgataacag cgatgtcctg gatgtttctg caccttttaa cattgagagt
     5341 tataaaacag cggaaggtca atatgctatt ccgtttaaag caaaatacct gaaactgaca
     5401 gataactcag tgcaatcagg tgatgtgtta tcttctctgg ttatgcgtgt ggcgcaggat
     5461 taataaataa aattgtaagt ttctccaggt aataaacatg gccgctatcc catggcggcc
     5521 ttttaattta agaggcatta aaatgaaagg attattatct ttactcattt tttctatggt
     5581 ccttcctgca catgccggaa ttgttatcta cgggacgcgc attatttacc cggcagaaaa
     5641 taaagaagtg atggtgcagt tgatgaacca gggaaaccgt tcttcgctgc tgcaggcgtg
     5701 gattgatgat ggcgatacgt cattaccacc agaaaaaatt caggttcctt tcatgttaac
     5761 gccaccagtg gcaaaaatag gggcaaattc cgggcagcaa gtaaaaatca aaattatgcc
     5821 gaataaactg cccactaata aagaaagcat tttttatctg aatgttctgg atattccacc
     5881 aaatagtcca gagcaagaag gtaagaatgc actgaagttt gcgatgcaaa acagaattaa
     5941 gttgttttac cgaccagcgg gtattgctcc ggtaaataaa gcgacattta aaaaattgct
     6001 ggtaaatcgc agtggcaatg gtttggtgat aaaaaatgac tcagctaatt gggtgacgat
     6061 ttcggatgtc aaagctaata atgtcaaagt caattatgaa actattatga ttgccccctt
     6121 agaaagtcag agtgttaatg tcaaaagtaa taatgcaaat aactggcatc tgaccattat
     6181 cgatgaccat ggcaactata ttagtgacaa aatttaattg caggagctgc ctatgttgag
     6241 aatgacccca cttgcatcag caatcgtagc gttattgctc ggcattgaag cttatgcagc
     6301 tgaagaaacc tttgataccc attttatgat aggtggaatg aaagaccagc aggttgcaaa
     6361 tattcgtctt gatgataatc aacccttacc ggggcagtat gacatcgata tttatgtcaa
     6421 taagcaatgg cgcgggaaat atgagattat tgttaaagac aacccgcaag aaacatgttt
     6481 atcaagagaa gttatcaagc ggttaggcat taatagcgat aacttcgcca gcggtaagca
     6541 atgtttaaca tttgagcaac ttgttcaggg tgggagctat acctgggata tcggggtttt
     6601 tcgtctcgat ttcagtgtcc cgcaggcctg ggtggaagaa ctggaaa
//
LOCUS       DAETQN010000187         6538 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.189, whole genome shotgun sequence.
ACCESSION   DAETQN010000187 DAETQN010000000
VERSION     DAETQN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.189"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            143..1318
                     /gene="mdtL"
                     /locus_tag="K0W68_004063"
     CDS             143..1318
                     /gene="mdtL"
                     /locus_tag="K0W68_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBI3075482.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1293..2252
                     /gene="yidZ"
                     /locus_tag="K0W68_004064"
     CDS             1293..2252
                     /gene="yidZ"
                     /locus_tag="K0W68_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBI3075483.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2409..3158
                     /locus_tag="K0W68_004065"
     CDS             2409..3158
                     /locus_tag="K0W68_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBI3075484.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3180..3730
                     /gene="yieF"
                     /locus_tag="K0W68_004066"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3180..3730
                     /gene="yieF"
                     /locus_tag="K0W68_004066"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3784..5121)
                     /gene="adeP"
                     /locus_tag="K0W68_004067"
     CDS             complement(3784..5121)
                     /gene="adeP"
                     /locus_tag="K0W68_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBI3075485.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5287..5952
                     /gene="yieH"
                     /locus_tag="K0W68_004068"
     CDS             5287..5952
                     /gene="yieH"
                     /locus_tag="K0W68_004068"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBI3075486.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6019..6492
                     /locus_tag="K0W68_004069"
     CDS             6019..6492
                     /locus_tag="K0W68_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBI3075487.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1608 a         1650 c         1652 g         1628 t
ORIGIN      
        1 aatttggcta aatccttcaa gaagccagcc attcgctggc ttcttgcctc tcaggaaatc
       61 acttatgtcc aaatggcaac tcgcctgatc ctccttcacc acgtatgctt tgcgtcacct
      121 tactatcagg acgctttagc ccatgtcccg ctttttgatt tgtagttttg ccctggtttt
      181 actttatccc gccgggattg atatgtacct cgttggttta ccgcgcatcg ccgccgatct
      241 caatgccagc gaagcgcagt tgcatattgc gttctccgta tatctggcgg ggatggcagc
      301 tgcgatgtta tttgccggta aagtggccga tcgttcaggg agaaagccgg tcgccatacc
      361 cggcgcggcg ctatttatta ttgcctcggt gttctgttca ctggctgaaa ccagcacgtt
      421 atttcttgca ggccgatttc tacaggggtt gggcgcaggc tgttgttacg tagtggcgtt
      481 cgccattttg cgcgacacgc tggatgatcg acgtcgggct aaagtgctgt cattactcaa
      541 cggtattacc tgcatcattc cggtgttagc gccagtgctc ggacatctga ttatgcttaa
      601 attcccgtgg cagagtctgt tctgggcgat ggcaatgatg ggcatcgcgg tactgatgtt
      661 gtctttgttt attttaaaag aaacgcgccc agcgtccccc gctgcttcgg acaaaccacg
      721 agaaaatagc gagtcgctgc ttaatcggtt tttcctcagc cgtgttgtta tcaccaccct
      781 cagcgtttcg gtgatcctca ctttcgtcaa tacatcgccg gtattgctga tggaaatcat
      841 gggttttgag cgcggagaat acgccaccat tatggcgttg accgctggcg tcagcatgac
      901 cgtttcattc tccacgccat ttgcgctggg aatttttaag ccacgtacgt tgatgatcac
      961 ctcgcaggtg ttattccttg cggcggggat cacccttgcc gtttcacctt cccatgcggt
     1021 ttctctgttt ggtatcacgc tgatttgcgc cggtttctcg gtaggttttg gcgtagcgat
     1081 gagtcaggcg ttaggaccat tttcattacg cgcgggcgta gccagctcga ccttaggtat
     1141 tgcgcaggtt tgcggttcgt cactgtggat ttggctggca gcggtggttg gtattggcgc
     1201 atggaatatg ctgatcggga ttctgattgc ctgtagcata gtgagcctgt tgctgattat
     1261 gttcgtcgcg cctggacgcc ccgttgccgc tcatgaagaa atccatcacc acgcttgatc
     1321 tcaatctgct gctctgtctg caactgctga tgcaggagcg cagcgtgacc aaagcggcga
     1381 agcggatgaa cgtgacccct tcagcggtga gtaagtcgct ggcaaagtta agagcgtggt
     1441 ttgacgaccc gctctttgtg aactcaccgc tgggcctgtc gcccacaccg ctaatggtca
     1501 gcatggagca aaatctggcg gaatggatgc aaatgagcaa tctgctgctg gataaaccgc
     1561 accaccagac accgcgcggc ctgaagtttg agttggcggc ggaatcgccg ctgatgatga
     1621 tcatgcttaa tgcgctgtcg aaacggatct accaacgtta cccgcaggcg accatcaaac
     1681 tacgtaactg ggattacgat tccttagatg ccattactcg tggtgaagtg gatattggtt
     1741 tttccggtcg cgaaagccat cctcgctcgc gggagctgtt aagctcgcta ccgttagcca
     1801 ttgattatga agtgctgttt agtgatgtac cctgcgtctg gttacgcaaa gatcatccgg
     1861 cactgcatga aacgtggaat ctggacacct ttttacgtta tccgcatatc agcatttgct
     1921 gggaacagag cgacacctgg gcgctggaca atgtgttaca ggagctgggg cgcgaacgca
     1981 cgattgctat gagtctgccg gaattcgagc agtcactgtt tatggcagcg caacccgaca
     2041 atctgctact ggcgaccgcg ccgcgctact gtcagtacta caatcaactc catcaactgc
     2101 cgttggttgc tcttcctctc ccgtttgacg aaagccagca aaaaaagctg gaagttcctt
     2161 ttaccctgct gtggcataaa cggaacagcc ataatccgaa gatcgtctgg ttacgggaaa
     2221 ccattaaaaa cctttacgcg tcgatggcat aaccgaatcg tatgaaacgc gacccaattt
     2281 cacaataaaa tgtaaaaaag ttgtaacaag cttgtctgaa tcgaactttt agccgcttta
     2341 gtctgtccat cattccagta aatgattact cttgtattca taatggacca ttaagcatgg
     2401 agcgaaaaat ggcgactcac tttgcccgag ggattttaac ggaaggacat ctgatttctg
     2461 ttcgtctccc ctcccagtgt catcaagaag cccgaaacat tccccctcat cgtcaaagcc
     2521 gttttctggc gtccagaggt ttactcgcag aactgatgtt catgctgtat ggcattggcg
     2581 aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt tttcagtgat aaaaatttgc
     2641 cttcgttttc tatttcctat gccgggaata tggttggcgt ggcgttaaca accgaaggtg
     2701 aatgtggcct cgatatggaa ctacagcgtg cgacgcgcgg gtttcatagc ccacacgcgc
     2761 ccgataacca caccttttcc agcaatgaat cgctatggat cagtaaacaa aacgatccta
     2821 acgaagcgcg ggcgcagctc atcacgctgc gccgaagcgt gctaaaacta accggtgatg
     2881 ttttgaatga cgatccgcgc gatctgcagc tgctgcccat tgccggacgc ctgaaatgtg
     2941 ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga cgtgctggtg tggtccgtgg
     3001 cggtcacgcc cgcgattgaa aagctcagtg tctgggagtt agatggcaaa cacagctgga
     3061 aaagcctgcc ggatattcac agccgcgcca acaatcctac cagccggatg atgcgttttg
     3121 cccagctctc taccgtgaag tctttttcgc caaattgata gacaaccaca ggagtcatta
     3181 tgtctgaaaa attgcaggtg gttacgttac tggggagcct gcgcaaaggc tcatttaatg
     3241 gcatggttgc acgtaccctg ccgaaaattg ctccggcgag catggaagtc aatgcgttac
     3301 cttccattgc cgacattccc ctgtatgacg ctgacgtaca gcaggaagaa ggttttccag
     3361 caacggttga agctctggcg gaacagatcc gtcaggctga cggtgtggtg atcgtcacgc
     3421 cggaatataa ctactctgta ccgggtgggc tgaaaaatgc catcgactgg ctttcccgct
     3481 ggccggtaaa ccggtgttga ttcagaccag ctcaatgggc gtgattggcg gcgcgcgctg
     3541 tcagtatcac ctgcgccaga ttctggtttt cctcgatgca atggtgatga acaagccgga
     3601 atttatgggc ggcgtgattc agaacaaagt tgatcggcaa accggagaag tgattgatca
     3661 gggtacgctg gaccacctga ccgggcaatt gaccgcattt ggtgagttta ttcagcgagt
     3721 taagatctaa ataaaaaacc cgccagtaat catgcatggc gggtttttaa tgcgctatcg
     3781 attttaatga gcgtcgataa atacaatctt caggataaac agcagcgcaa cgatgattac
     3841 gcacgggcta aggtcacgca gacgcccggt accaatcttc atcacgcagt aggagataaa
     3901 gcccagcgca ataccttcgg taatcgaaaa gctgaacggc atcatcacgg cggtaataaa
     3961 cgccggaaca gattcagtaa gatcctgcca gttcacgcga gcaagacttg aggtcatcaa
     4021 cacgccaacg tagatcaacg cgccagctgc agcgtagcct ggcaccattc ccgccagcgg
     4081 cgacagaaag ataaccagca ggaacaacag accaacaacc actgccgtca gaccggtacg
     4141 accgccaacc gatacgccgg aagaggactc aatataagcc gtaacggaag aagtaccgat
     4201 aaacgaaccg gtcacggaag agatactgtc gacatacagc gcctgcttca tgcgcgggaa
     4261 tttccccttc tcatccgcca gacctgcttt atcggtcacg ccaatcagcg taccggagga
     4321 gtcaaacaag ttgaccaaca tgaaagagaa aatcacccct gccagcccga ggttaaacga
     4381 cccggctaaa tctacatgac ccacaactgt cattacgctc ggcggcgcag aaacgatgcc
     4441 attgtagtgc acatcaccca gcatccagcc cagcagcgtc gtcaccacga tagaaaccag
     4501 caccgctgcg tgaatgttgc gcgaggccag aatggcgatg atgaagaaac cgaggatccc
     4561 cagcagcacg ctgtgagaag tcagattacc gatgctcacc agcgtttccg ggttagcgac
     4621 aatcacacct gcgtttttca gccccatcat gccaatgaac agaccgatac cgctggtaat
     4681 acccacacgc agacttaccg gaatgttagc aatcatccag taacgaacgc ggaaaatggt
     4741 caaaagaagc agtccgacag cgccccagaa gattgcgccc ataccgatct gccacggcag
     4801 ccccattgcc tggacaacca caaaagcgaa aaaggcgtta agccccatcg caggagccag
     4861 cgccaccgga aggtttgcaa acaatcccat cataatgcta ccgaaagcag caatcagaca
     4921 ggttgttacg aagacggcac tggtgtccat gccagcaacg cccagaattt gtgggttaac
     4981 aaatacgatg tagaccatcg tcaggaaggt ggtgaacccg gcaatcactt cagttcgtgc
     5041 cgtagtacca tgttcgcgca gtttaaaaac tcgttccagc atcccctgac cagaagtctg
     5101 ggtggtatgt tgatgactca ttatctattt ccgaacaagg agggaaaatc cgtcgctatc
     5161 gtataccaaa atgcgacaat aggcgcgttt gtgagagact ttttttattg gatttactta
     5221 tacggcaacg attgcgttgc gcaaatcggc tctacgaaaa cgttaaactg attaaaaagg
     5281 aaaggcatgt cccggataga agcggtattt ttcgactgcg acggtacgct ggtcgacagt
     5341 gaagtcattt gctcccgcgc atatgtaacg atgtttcagg aatttggtat tacgctcgat
     5401 cctgaagagg tattcaaacg tttcaaaggt gtaaaactgt acgaaattat cgatattgtt
     5461 tcccttgaac atggtgttac gttagcgaaa acagaatctg aacacgttta ccgtgcagaa
     5521 gtcgctcggc tgttcgattc agaactggaa gccatcgaag gggctgaagc gctcctgtca
     5581 gcgatcactg cgccaatgtg tgtggtatct aacggcccaa ataacaaaat gcagcattct
     5641 atgggcaagc tgaatatgtt gcactacttc ccggataaac tgttcagcgg ctacgatatt
     5701 cagcgctgga agccagaccc ggcgttaatg ttccatgcgg caaaagcgat gaatgtaaat
     5761 gtagaaaact gcattctggt tgatgactca gttgccggtg cgcaatctgg tatcgacgca
     5821 ggtatggaag tgttctactt ctgcgccgac ccgcacaata agccgatcgt tcacccgaaa
     5881 gtcaccacct ttacccatct ttcgcagtta ccggaactgt ggaaagcgcg tggttgggat
     5941 attacggcat agttcttcac actcccttca cttaccccgc ttaaattggc gctcaaaggt
     6001 aagtaaaggg agtttgatat gtctgtttca cgtcgggtaa tacatcacgg actttatttt
     6061 gcggttttag gaccgttaat tggtgttctg tttcttgtcc tctacatatt cttcgcaaaa
     6121 gaaccgctgg ttcttttggt aataatacag gtacttccat tgtttctctt attgtcgatt
     6181 actacgggag ctattcctgc attgttaacc ggcgtgatgg tcgcctgtct gccggaaaag
     6241 atcgggtcac agaaaaatta tcgttgtctg gctggtggca taggtggcgt cgttatcacc
     6301 gagatctatt gtgcagttat tgtacatatt aagggcatgg cttcctcgga gttgtttgaa
     6361 aacattcttt ctggtgacag tctcgttgtc cgcatcattc ctgcattgct ggcaggtgtg
     6421 gtgatgagca gaatcattac ccgtctgccc ggattggata tttcatgtcc tgaaacagac
     6481 tctttaagtt aagcgggata ctttatcttt gggctactca aaagcagaca ggatgtta
//
LOCUS       DAETQN010000188         6532 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.190, whole genome shotgun sequence.
ACCESSION   DAETQN010000188 DAETQN010000000
VERSION     DAETQN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.190"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..906)
                     /locus_tag="K0W68_004070"
     CDS             complement(<1..906)
                     /locus_tag="K0W68_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HBI3075488.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
     gene            1602..3887
                     /gene="nrdA"
                     /locus_tag="K0W68_004071"
     CDS             1602..3887
                     /gene="nrdA"
                     /locus_tag="K0W68_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBI3075489.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            4121..5251
                     /gene="nrdB"
                     /locus_tag="K0W68_004072"
     CDS             4121..5251
                     /gene="nrdB"
                     /locus_tag="K0W68_004072"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBI3075490.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            5251..5505
                     /gene="yfaE"
                     /locus_tag="K0W68_004073"
     CDS             5251..5505
                     /gene="yfaE"
                     /locus_tag="K0W68_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBI3075491.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="K0W68_004074"
     CDS             complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="K0W68_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBI3075492.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRNKAINHDFRQLENIWSQSPKRTGSR"
     gene            6387..6532
                     /locus_tag="K0W68_004075"
                     /pseudo
     CDS             6387..6532
                     /locus_tag="K0W68_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1686 a         1686 c         1526 g         1634 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tcataattca agttaataac cttaagggat accagttata
     1081 tttaaactaa attatagtaa tgaataattt tcttataata taaggtaaat taacaaaatg
     1141 gcttcgcatt taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac
     1201 attcgcttat atattgacca caactgatac atcagattat gtgatgactc gtgcttagat
     1261 caatttttgc aatcattaac aaaaagatta ataaaccatc tatatcaatt tatccaacct
     1321 attctgccgt tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg
     1381 actttcccgg acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt
     1441 tatccacaaa gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc
     1501 aaacataccc cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg
     1561 aaactattgc ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg
     1621 tgacaaagcg cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg
     1681 attgggcggc agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca
     1741 ttcagtttta tgacggtatc aagacctctg acatccacga aaccattatc aaggctgccg
     1801 cagacctgat ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct
     1861 tccacctgcg taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg
     1921 tgaaaatggt cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag
     1981 agttcaagca gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg
     2041 ccgttaagca gctggaaggc aaatatctgg tacagaaccg cgtgaccggc gaaatctatg
     2101 agagcgccca gttcctttat attctggttg ccgcgtgcct gttctcgaac tacccgcgtg
     2161 aaacgcgcct gcaatatgtg aagcgttttt acgacgcggt ttccacattt aaaatttcgc
     2221 tgccgacgcc aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac
     2281 tgatcgagtg cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgttaaat
     2341 acgtttccca gcgtgccggg atcggcatca acgccgggcg tattcgtgcg ctgggtagcc
     2401 cgattcgcgg tggtgaagcg ttccataccg gctgcattcc gttctacaaa catttccaga
     2461 cagcggtgaa atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc
     2521 cgatgtggca tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggtgtggaag
     2581 gcaaccgcgt gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgtc
     2641 tgctgaaagg tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg
     2701 cgttcttcgc cgatcaggaa gagtttgaac gtctgtatac caaatatgag aaagacgaca
     2761 gcatccgcaa gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg
     2821 cgtctaccgg tcgtatctat attcagaacg ttgaccactg caatacccat agcccgtttg
     2881 atccggccat cgcgccagtg cgtcagtcta acctgtgcct ggagatagcc ctgccgacca
     2941 aaccgctgaa cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt
     3001 tcaacctggg cgcaattaat aacctggatg aactggaaga gctggcaatt ctggcggttc
     3061 gtgcacttga cgcgctgctg gattatcagg attacccgat cccggccgcc aaacgtggag
     3121 cgatgggtcg tcgtacgctg ggtattggtg tgatcaactt cgcttactac ctggcgaagc
     3181 acggtaaacg ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcca
     3241 ttcagtatta cctgctgaaa gcctctaatg agctggcgaa agagcaaggc gcgtgcccgt
     3301 ggtttaacga aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc
     3361 tggataccat cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca
     3421 aaacgcacgg cctgcgtaac tctacgcttt ctgccctgat gccgtccgag acctcttcgc
     3481 agatctctaa cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt
     3541 cgaaagacgg tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg
     3601 agctgctgtg ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga
     3661 aatttatcga tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag
     3721 gaaaagtgcc gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca
     3781 aaacgctgta ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac
     3841 cgtcaatcca ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg
     3901 gatgcggcgt aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc
     3961 cagcgtcgca tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc
     4021 tcggatttgt aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa
     4081 aatgccttat ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc
     4141 acagacgaaa aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc
     4201 tcgctacgat cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt
     4261 cttctggcgt ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc
     4321 ggagcacgaa aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat
     4381 tcagggtcgt agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac
     4441 ctgggtcgaa acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat
     4501 tcgtaatatc gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat
     4561 ccagaaacgt gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta
     4621 ctggcacctt ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct
     4681 gcgcgagctg aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat
     4741 ccgcttctac gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg
     4801 taacgccaag attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca
     4861 gcatatgctg aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga
     4921 agaatgtaag caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg
     4981 ggcggattat ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca
     5041 gtacgttgaa tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca
     5101 gacgcgctcc aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt
     5161 tgctccgcag gaagtggaag tcagttctta tctggtcggg cagattgacg cggaagtgga
     5221 caccgacgat ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc
     5281 acacaactgc tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat
     5341 gttgcggttg agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc
     5401 gcaggtcagg tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg
     5461 ccctgttgtt gccgggcaaa aggcgatatt gaaatcgaga tgtgaattgg ttgtagtgcc
     5521 agatacaacg cttatacgtc ttatctggcc tacaacgatt acatggcgta gtaatacgct
     5581 ttcacctgct cccagtccgc tttggggatt ggctccagat attttccagc tggcggaagt
     5641 catgattaat cgctttattg cgacgcaagc ggcgacgact tttctccagg tcaagaaaac
     5701 cagcttctgc tttaccttct gttttcacat agatatggcg aacataacaa cagccatgct
     5761 gacgattaac gctatgcatt ttcttaaacg ccagcgccac tgctttcaac atggcttgcc
     5821 gtacttcgtc agaatagggc gatactgcat gccgggcata ccagtcagca atgctgatga
     5881 accccgccat atcttcagtc accagcaacg cgcgccattc accctcaatt ttcaccgctt
     5941 caccaaaaac gatcttcggc acgatgacac cggcccgttc cagttcttta attaccgcaa
     6001 cctcacggac aatcgttggt cggccgaacg gataacgtac ggaatgaaac agatgatgcg
     6061 tcatgcgctt tacatacagc tttttgccgt tgcgctcgac gcattgcacc ccgctcatac
     6121 cattacggcg atagttaggc tcttcaaccc agtcgccctc tgttgcccac cagtgattaa
     6181 attcgtcgta ctttgctgaa actgccatac cctatcgcct gtcattttta ttaacgacaa
     6241 tgactatagg tggttacctg aggaaaatct taatgaaacg tgtcgtatta atgaatttct
     6301 tttgccgcta ctagtgtgac accgcttttg cccttcattt gcagcgcata gattccacca
     6361 gccacgacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag gtggatacag
     6421 cgatgaattt tattcgacag ggattaggca tcgccttatt accagagtta acgctgaaaa
     6481 ccattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga ca
//
LOCUS       DAETQN010000189         6515 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.191, whole genome shotgun sequence.
ACCESSION   DAETQN010000189 DAETQN010000000
VERSION     DAETQN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.191"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            38..250
                     /locus_tag="K0W68_004076"
     CDS             38..250
                     /locus_tag="K0W68_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075493.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="K0W68_004077"
     CDS             283..501
                     /locus_tag="K0W68_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBI3075494.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            656..889
                     /locus_tag="K0W68_004078"
     CDS             656..889
                     /locus_tag="K0W68_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBI3075495.1"
                     /translation="MVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQRSLSERLFRE
                     QGVDTKILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="K0W68_004079"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="K0W68_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBI3075496.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="K0W68_004080"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="K0W68_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="K0W68_004081"
     CDS             1917..2660
                     /locus_tag="K0W68_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBI3075497.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="K0W68_004082"
     CDS             2690..3229
                     /locus_tag="K0W68_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBI3075498.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="K0W68_004083"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="K0W68_004083"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBI3075499.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="K0W68_004084"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="K0W68_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBI3075500.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="K0W68_004085"
     CDS             4582..6102
                     /locus_tag="K0W68_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3075501.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..>6515)
                     /locus_tag="K0W68_004086"
     CDS             complement(6135..>6515)
                     /locus_tag="K0W68_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075502.1"
                     /translation="IANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKK
                     NTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQ
                     ASKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1714 a         1489 c         1550 g         1762 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gcaat
//
LOCUS       DAETQN010000190         6383 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.192, whole genome shotgun sequence.
ACCESSION   DAETQN010000190 DAETQN010000000
VERSION     DAETQN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.192"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            6..326
                     /locus_tag="K0W68_004087"
     CDS             6..326
                     /locus_tag="K0W68_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine transporter"
                     /protein_id="HBI3075503.1"
                     /translation="MTMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASY
                     RYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKD
                     SQRA"
     gene            428..1882
                     /gene="amn"
                     /locus_tag="K0W68_004088"
     CDS             428..1882
                     /gene="amn"
                     /locus_tag="K0W68_004088"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBI3075504.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2213..2929
                     /locus_tag="K0W68_004089"
     CDS             2213..2929
                     /locus_tag="K0W68_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI3075505.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3382..3457)
                     /locus_tag="K0W68_004090"
     tRNA            complement(3382..3457)
                     /locus_tag="K0W68_004090"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3422..3424),aa:Asn,seq:gtt)
     gene            complement(3558..5201)
                     /gene="yeeO"
                     /locus_tag="K0W68_004091"
     CDS             complement(3558..5201)
                     /gene="yeeO"
                     /locus_tag="K0W68_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HBI3075506.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            5206..5281
                     /locus_tag="K0W68_004092"
     tRNA            5206..5281
                     /locus_tag="K0W68_004092"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5239..5241,aa:Asn,seq:gtt)
     gene            complement(5319..6269)
                     /gene="cbl"
                     /locus_tag="K0W68_004093"
     CDS             complement(5319..6269)
                     /gene="cbl"
                     /locus_tag="K0W68_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBI3075507.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT         1814 a         1542 c         1372 g         1655 t
ORIGIN      
        1 gagtcattac catggcgctt gaagcacaat ctattttctg gatagttttc ttctccataa
       61 tgctggcaaa cattgcgcat gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa
      121 tgtttggtgc cagttatcgc tatagtggcg ctggagtcgg ttatcaggtt gccagtgtgg
      181 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      241 ggcatagcgt cgccatttat ttgctggctg gatgcctgat ttccgcaatg accgctttgt
      301 tgatgaaaga cagtcaacgc gcttgatagc ctggcgaaga tcatccgatc ttcgccttac
      361 acttttgttt cacatttctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      421 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      481 cgcgctgtat gagcaatctg tagtcgcatt acgcaacgcc attggcaact atattacaag
      541 tggcgaatta cctgatgaaa acgcccgcaa acaaggtctt tttgtctatc catcactgac
      601 cgtaacctgg gacggtagca caaccaatcc ccccaaaacg cgcgcatttg gtcgttttac
      661 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatcttaa
      721 tgaacaactt acgttgctgt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      781 gcatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      841 gagcgctggg ttaactcgct acttcccgac aacagaactg gcgcaaattg gcgatgaaac
      901 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      961 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagttg aacattttca
     1021 gccgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
     1081 cagccagatc ctcgatcctg atagtcctta cattgccctt tcttgtgctg gcgggaactg
     1141 gatcaccgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1201 ccagatgcca gcatggaatt taattaccgc cgatgctcag ggtattactc tggtgaatat
     1261 tggcgtggga ccatcaaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1321 tgtctggttg atgattggtc actgtggcgg attacgtgaa agtcaggcca ttggcgatta
     1381 tgtacttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1441 tattcctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt
     1501 gagcggcagg cccggtgagg aagtcaaaca gcggctacgt actggtactg tggtaaccac
     1561 agatgacagg aactgggaat tacgttactc agcttctgca cttcgtttta acttaagccg
     1621 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1681 gccatacggg acactactgt gtgtttcaga taaaccatta catggtgaga ttaaacttcc
     1741 cggtcaggct aaccgttttt atgaaggcgc tatttccgaa catctgcaaa ttggcattcg
     1801 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt
     1861 taatgagccg ccgttccgat aataagttct gaaccttctt atcagaaata gatgtgaagg
     1921 agtaagtaag acacctggca aatagcctgc aataatcgtg ggctgtttgc ttccttgggc
     1981 ggatacgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     2041 aaacacaatt ataaaaaatc ccgccaacaa tataagtttt tataaaatta tggctttaga
     2101 atatttttat ttctaataga cgagattttt cctgttatga tataatatgc cgaattaaca
     2161 catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg
     2221 taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta
     2281 tgcaaaattc ggtgtagaaa tctatgctgc tgctaaacaa ggtgaacccg atccagaatt
     2341 aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt
     2401 tattgataaa gcaattgata aagccaaagg cggcggagat gaaacgttcg tgcagggacg
     2461 ttatgaaggc tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt
     2521 taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc
     2581 ggcaggttct gtcagctata tgtttgacaa tgctggtgtg attgtattta aaggatcaga
     2641 ccctgaccat atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga
     2701 agaagaaggt aacattgtta tttataccga acctactgac cttcataaag gaatcgcggc
     2761 tctaaaagca gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc
     2821 tgaagttgag ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga
     2881 agatgacgac gatgtacaaa aagtttatca taacgtcgca aatctctaat tatcttttaa
     2941 agaaatctgt ctttacggca gatttcttta atctcatata attcttataa aaaatataag
     3001 attcaactcg tcatattgat tatacccccc cgttcccaga gaaataatat ttattaaaat
     3061 tccagttctt ctttttctga ttacagaagg caaagtggca attacgcata gtttcccgat
     3121 aaagacgcga tagcgacatc ccgcataagg catttttctc tttatctttg tacggtactt
     3181 catggaacag agtttttgac cttgcgaatc gtgatgtctg ttggggaggg gcaatttgct
     3241 cactgaagcg tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggtcact
     3301 tttttacaag gtaaccgggt aaaaataatt tttatttttt aactgttttg agactcatag
     3361 agatgtctca aaactaaaat ttggctcctc tgactggact cgaaccagtg acatacggat
     3421 taacagtccg ccgttctacc gactgaacta cagaggaatc gtgagaacga ggcgaatatt
     3481 agcgatgccc acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt ttgctgaaat
     3541 aatctgcatt ttgtcgttta ttccgacaca actggctttt tttcacactt ttgcggttcg
     3601 gatcgagggt atttccacag ccaacgtcca gtaaccattc gccagtaaaa cagcacggcc
     3661 cgcacagccc agtcggcaaa cattcccatc cagacaccaa ccacacccca gccaagcatg
     3721 atgcccagca cataaccgac gacaacccgg caaccccaca tgctcaacat agaaacccac
     3781 atggcgtaac gggcatcgcg agcaccttta aatccagcgg gtagcaccca tgaggcggac
     3841 caaataggca taaataaagc atttagccaa atcagaatca caacgacatg tttaacctgt
     3901 ggatcctggg tgtaaaacga tgccataacc ccggcaaagg gagccgttag ccaggcgatg
     3961 gccgttaatc caagagtgga aagccagaac acatgccgca actgaatctc tgcttgcgct
     4021 atctgcccta cccccaacct tcggcctgta atgatcgtag aagcagagcc gagcgcactt
     4081 ccgggtaagt tgataagagc cgcaattgaa aacgcgataa aatttccggc aataacactg
     4141 gtccccatcc cggcaacgaa catttgggtt aataaccgac cactggtaaa taacactgat
     4201 tcgacactcg cgggaatacc aatccccatg acttcccaga taatgctaaa attcagcggt
     4261 ttaaaatagc tctttaacga aatccttagc gcaggattaa aaccaatcgc cagcacccac
     4321 aaaattgcaa ctgcgccaat ataacgagaa atggttaaac ccagccctgc cccgacaaat
     4381 cccagtcccg gccaggagaa aaggccgtaa atcaatatgc cgctaataat aatattaaga
     4441 atattcaggc taccgttaat caatagcggt attttcgtat tccctgcacc acgaagtgcc
     4501 ccgctaccaa taagagtgat ggcagctgct ggataactga gtaccgtcag ctccagataa
     4561 gtcaacgcca gtgctttaac ttctgtcgtg gcatcacccg cgacgaaatc aataatttgt
     4621 tcgccaaaat gatgaataag cgttgccaac agtacggcaa acaacgtcat gatcaccaat
     4681 gactgccgcg tcgccaccct cgctcgtcgt cgatcccgct taccgagact aaatgccaca
     4741 acgacagtag taccaagatc gatagcagca aaaaaagcca taatgaccat attgaagctg
     4801 tccgccaatc ccacgccggc catcgcatct tttcccagcc agctgaccag aaaagtgctc
     4861 agaaccccca tcaacaggac acaggcattc tccatgaaga taggaacagc aagcggggtt
     4921 atctcgcgcc agaacaacac tttgtagctc ttgcgtttag cgtgccagcg agtgccgtga
     4981 acaacctggc gtaaagcaga ggagatattc aaagccgacc ttaattgcag aaagtgaaac
     5041 cacatttcaa ataatgaggg agaatcagca aagctgcaaa gattttcgcc aacaaattgt
     5101 ctgcaaatgc aacaaactgt tgatagaaac ggcaaacagt tggggaattt aaaaatcggg
     5161 tttgacaaaa gatttttcgc cgttaagatg tgcctcaaca gcgattcctc tgtagttcag
     5221 tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc
     5281 aaatttctga aaagcccgct tttatagcgg gctttttgct atatctgata atcaatttcc
     5341 tcttcactgt tttccatcac ctgccgcttg atatcctcaa ctgacagtcc tgcattacaa
     5401 agttccagaa agcgccagac atagttacgc tgaagttgtc ctcgcttcag tcccaaccag
     5461 acagtattag catcgaaaag atgccgcgta tccagacgga ttaaattctc ttcctcttgt
     5521 tcgccgctgg attgttcggc aactaatccg atcccaagcc caagagcaac ataggtttta
     5581 atgacatcag aatcctgcgc acttaataca atatctgcca gcaaaccttt gcgggcaaat
     5641 gcgtcatcaa tacgtgagcg ccccgtaatc ccctgtcggt aagtgattaa cggccacttc
     5701 gctattgatt ccagcgtcaa tggtgtaatt tgcgttaagg gatgatcgag tggaacaagc
     5761 aaactatggt gccaacgaaa ccacgggaag gcgacgagct gcgggtcatt actcaaacgc
     5821 tcgctggcga taccaatatc agcttcgcca ttttgcaaca acgtcgcaat ttcctgtggc
     5881 gtcccctgga ttagctcgag ccgaacctcc gggaaaagtt cgcgaaaagc tttaatgacc
     5941 tctggcaagc tataacgtgc ctgagtatgc gtcgttgcaa tagtgagaac gccagacgta
     6001 tcgttggtaa acaggtctgc aagccgacga acattactgg cttcattcag aatacgttct
     6061 gcaatgacca gtaatgcttt gcccggttca gtcatgccca gcagtcgctt acctcgtcga
     6121 acaaatattt cgatgccaag ttcatcctcc agttcccgaa tatgacggct gacgccggac
     6181 tgtgaggtaa aaagcatatt cgcaacctct gtcaggttgt aatcctgacg tgcagcctcg
     6241 cggattatct ttagttgttg gaaattcacg gtaaactccg ggcagttcag atttcccgtt
     6301 attgttaaag tctaatgccc ggcataacaa ataataaaaa cccgcatctt attccatccc
     6361 gatataacgc ttagctcacc aat
//
LOCUS       DAETQN010000191         6325 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.193, whole genome shotgun sequence.
ACCESSION   DAETQN010000191 DAETQN010000000
VERSION     DAETQN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.193"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..355
                     /gene="mcbR"
                     /locus_tag="K0W68_004094"
     CDS             <1..355
                     /gene="mcbR"
                     /locus_tag="K0W68_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBI3075508.1"
                     /translation="TPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNM
                     PILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAE
                     IMQQNIAILYQQYNR"
     gene            complement(391..2493)
                     /locus_tag="K0W68_004095"
     CDS             complement(391..2493)
                     /locus_tag="K0W68_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBI3075509.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2735..3796
                     /locus_tag="K0W68_004096"
     CDS             2735..3796
                     /locus_tag="K0W68_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBI3075510.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3911..5410)
                     /gene="ansP"
                     /locus_tag="K0W68_004097"
     CDS             complement(3911..5410)
                     /gene="ansP"
                     /locus_tag="K0W68_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HBI3075511.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5677..6294
                     /locus_tag="K0W68_004098"
     CDS             5677..6294
                     /locus_tag="K0W68_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HBI3075512.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1704 a         1594 c         1513 g         1514 t
ORIGIN      
        1 caccccgcaa gacctgacgg aactccagga attgctcgag aaattacagc aggcgcaaga
       61 aaagggcgac atggaacaaa tcattaatgt aaacaggcta tttcgcttag cgatttatca
      121 tcgctcaaat atgcccatcc tgtgtgagat gattgagcaa ctgtgggtca ggatggggcc
      181 tggtttacat tatctctatg aagcgattaa tccagcggaa ttacgggagc atatagaaaa
      241 ctatcatctg ttactcgctg cgttaaaagc aaaagacaaa gagggatgca gacattgtct
      301 tgctgaaatt atgcaacaaa atattgctat tttataccag caatacaatc gttaaagtaa
      361 atgcccggca aataccgggc atcaaggtaa ttactcaaat ctccacgcaa tattcatgcc
      421 gacaccataa tttcgtccgg gcgaaggttc gtaatatcgc ccgtttgact cattgacaat
      481 gacagaacca acgtattctt tatcgaataa attatcgaca cgaccaaata aatccacagt
      541 taaattgtgg taattgtatt tatacccggt gaataagccg acgagagtat aagacggcgc
      601 ttttgccgta ttttcatcat ctgccataat atcgcccata taacgcgctt ccgtgcctgc
      661 ataccaacca tcttccggta cataacctat cgacgcaaag cccatattac gggcgatccc
      721 tggcatccga ttaccgttac aatcctgttc attgcaaaca ttgctgcgat aggtcgcatc
      781 aagccaggtc caggacgcgt ttacgcgaaa atctcctgcg aaacgttgat cccatgccag
      841 ttcagcgcct tgacgacggg tctttccggc atttttgtaa gtcgtacgcc caccgctact
      901 gctatcgaca acaatttcat catcagtgtc ggtctgaaac aatgcgagac taagcagccc
      961 atcaccaata cgcgttttac tgccgatctc aattgtatcg ttggtggatg gttttaaacc
     1021 aaagttcata ccgctttgcc catcagcacg ataagacagc tcattaatcg tcggcgtttc
     1081 aaaacctcgc ccggctgcca gatagatatt ccaggcatcg gtcattgcat attttaacga
     1141 accggcaggt agccatttat gataactggc atcaccgctg tcatcgccgt tacccggagt
     1201 aacgtaatgg tcgttggaat caaaccacac ggagctgtag cgcacgccag catccagcga
     1261 cagtttttcg ctcagctgcc actgcgtctg taaataggga tcgatgttcc acatcagatt
     1321 gcgttcgtcg cgacgcaact cacctttttg cccgtactcc ggcatgccgc tattcaggcg
     1381 gaagttattg tagcccttgc ggttttcact catgttttcg tagttcaggc cggtagtgaa
     1441 cgtgaccgga acgcccagtt caccacggtg tgtccagcgg ctgtctatcc cctggtaatg
     1501 gcgttgcagg gtaatcacgc cgcccgcatg tgacgggtta agttgtggtg ccatgggtat
     1561 tgactggtac tgggtcgttt ctcgctctcc ggcatacatc atcacactca tatcatcttg
     1621 cgcgctcagg ctacgctcat agcgcaaccc agcctgagtt tgcttgatgg tttttcgcgt
     1681 gtcgtactgt tctgcacgag gcgcttgttg tggattagcc ttccattctg ctttggttag
     1741 cccacccggg tcatctgctt tgatatccac actattgaaa atcagactta atttgctggc
     1801 ttcatcaatg cgtacgccca gtttggcatt ggctaaattt ttctgtgcgc cactatggtc
     1861 acgatagccg tgggtcgtaa aacgcgtggt tgagacggtg taatcgacat cgccaggctg
     1921 tgtgccgtct cccgttgcgc ccgttgcttt cagcccatag cgccagctgc caaaactgcc
     1981 gtagtaacta ctggcttcaa tggttggtgg ctgttgtccg gtctgggtgg tgacattcat
     2041 taccccacca gacgcgttgc catacagggc agagaagggg ccacgcagca cttccacatt
     2101 ttgcacactg cttaaatcga tgttggatgt ttgcccttgc ccgtcgggca tggtggcggg
     2161 aataccgtcc acatacaggc gaataccgcg aataccgtaa gtggagcggg agccaaatcc
     2221 gcgaatcgac agctgtaaat cttgcgcata gttctgccgg ttttgtacct gcaaaccagg
     2281 cacgtcggtc agtgattcgg acaagttaat gcgcggtgtt gccaggcgca tctcctcgcc
     2341 atccaccacg cttactgctg ctggggtatc cagttctgaa accacctgcg gtgcggcact
     2401 gacaatcatg gtctgttcat cagcggcaaa aacaacgggg gaaagggcaa gtagtgcggg
     2461 caaaacggtc tgtcggacgg aaaaaatctt catgaaaaaa gccaggttaa gaatgggaaa
     2521 acgccgttat ggtaataaaa ttgtaaattc atggaaaacg aaacggcacg atacgttaag
     2581 taattgagaa aattgtagtc gtaacggcaa gaaatgctcc acatttgaga aaataatgat
     2641 taccattccc atttataaca agagcgtaac gatgattacg cttagcgaag cattgtgaag
     2701 cagcaaaaat atcggttcat caaagggagt cgtcatgcat ttacgtcatc tgttttcatc
     2761 gcgcctgcgt ggttcattac tgttaggttc attgcttgtt gcttcatcat tcagtacgca
     2821 ggccgcagaa gaaatgctgc gtaaagcggt aggtaaaggt gcctacgaaa tggcttatag
     2881 ccagcaagaa aacgcgctgt ggctcgccac ttcgcaaagc cgcaaactgg ataaaggcgg
     2941 cgtggtttat cgtcttgatc cggtcactct ggaagtgacg caggcgatcc ataacgatct
     3001 caagccgttt ggtgtcacca tcaataacac gactcagaca ctgtggtttg gtaacactgt
     3061 aaacagcgcg gtcacggcga tagatgccaa aacgggcgag gtgaaaggcc gtctggtgct
     3121 ggatgatcgt aagcgtacgg aagaggtgcg cccgctgcaa ccgcgtgagc tggtagctga
     3181 cgatgccacg aacaccgttt acatcagtgg tattggtaaa gagagcgtga tttgggtcgt
     3241 tgatggcgag aatatcaaac tgaaaaccgc catccagaac accggtaaaa tgagtaccgg
     3301 tctggcgctt gatagcaaag gcaaacgtct ttacaccact aacgctgacg gcgaattgat
     3361 taccatcgac accgccgaca ataaaatcct cagccgtaaa aagctgctgg atgacggcaa
     3421 agagcacttc tttatcaaca ttagccttga taccgctagg cagcgtgcat ttatcaccga
     3481 ttctaaagcc gcagaagtgt tagtggtcga tacccgtaat ggcaatattc tggcgaaggt
     3541 tgcagcaccg gaatcactgg ctgtgctgtt taacccagcg cgtaatgaag cctacgtaac
     3601 gcatcgtcag gcaggtaaag tcagtgtgat tgacgcgaaa agctataaag tagtgaaaac
     3661 gttcgatacg ccgactcatc caaacagcct ggcgctgtct gccgatggca aaacgctgta
     3721 tgtcagtgtg aaacaaaaat ccactaaaca gcaggaagct acccagccag acgatgtgat
     3781 tcgtattgcg ctgtaataga tatggcgagg gtgaaaacgg agagacttgc ctgactttta
     3841 tcacgccgca tgcggcacac cttcgtcagt aatctaaatt tgcccggcac gtagccgggc
     3901 aattttgcaa ttagctcgcc gtttcaggct taaacacaat ttcctgtttt tcttcatctt
     3961 cctcgacgac tggcgcagtg ctgtgaattt cagcaacgcg tttgcgcaca ccaaaccagc
     4021 ctataaccag cagaataccg ataatcggca gcgccgcgat agtataagtc ccgttcgggt
     4081 aatcgaacgc catcagcaca aggacactca gtaaaaacag taatgtcagc caggaagtga
     4141 agggcgcgcc aggcagttta aaactgacat ccgctgcttt gccttcttta atcgctttac
     4201 gcaggcgcat ctggcacacg atgataaacg cccatgaagc gatgattccc agcgacgcga
     4261 agttcaacac aatctcaaat acgcgcgacg gcaccagata gttgaggaat acgccgacga
     4321 catacacaac cagtgtcgcc agaatcccgg catacggcac atgctgacga ctcattttcg
     4381 ccataaaact cggtgcggaa ccgcccatcg ccatcgagcg cagaatacgc ccggtgcagt
     4441 acagacctga gttcaggctg gagagggcag cggtcagcac cacaatgttc ataatgctgc
     4501 cgatatatgg cacacccagt ttagagaaaa acgtcacgaa cggactttgc cccgcctgat
     4561 acgcgctcca cggcaataac ataaccagca acaccacgga gccgacgtaa aacaggccaa
     4621 tacgccaaat cacactgtta atggctttag gcaccatggt ctgcggatct ttacattctc
     4681 cggcagctgt acccaccatt tcaatggagg caaaagcaaa cactacgccc tgaatcaaca
     4741 ccagcgcagg cagcaaaccg tgggggaaga agccgccatt atcggtgatt aaatgaaagc
     4801 cagtggtgtt gccatccagc ggctgaccac taccgaggaa cactgtaccc acgaccaaaa
     4861 aggtcacaat ggcgagcact ttaataagcg caaaccagaa ctccatctcc gcaaaccatt
     4921 tcacaccgat catattcatg gtgccaacga tggtaagtgc agcgagcgca aagacccact
     4981 gcggcacgcc gccaaacgca ccccagtaat gcatatacag agcgacggcg gtaatatcaa
     5041 caatccccgt catcgcccag ttgatgaagt acatccagcc agcaacataa gcggctttct
     5101 cacccaaaaa ctcacgggca taagagacaa aactgccact ggaagggcgg tgtagcacca
     5161 gctcacccaa tgcacgcaga ataaaaaacg aaaacaagcc acaaattaaa taaaccagtg
     5221 ccagtgctgg acccgccatt tgcagtcggg ctcctgcacc taaaaacaag ccggtgccaa
     5281 tcgcgccgcc aatggcgatc atctgtacct ggcgattgcc catcgctttg tgatacccct
     5341 cttcgtgggc attaagccag cggcgtttcg cggcgtgttg atctgaagtg tcggtgtcgt
     5401 gtttactcat tgctctccct gattgcttta atgaaaaagt catataagtt gccatgaaca
     5461 atgtttattc atcctggcga tgatagttat caccattatt ctttatgggt ataaaactat
     5521 cggcgcagca tcctacccgt tatcgatata cgatgcaaaa catcccctta caatcctgaa
     5581 ggggattcat acaactgacg aaaaaatgac aaatactttt gctggttaac ctgtgtactg
     5641 tcctacactt aatctttaaa agattgtgag gggcatatga ttaaggtgta tggcgtaccc
     5701 ggatggggct ctacaatcag tgagctgatg ctaacactgg ctgatattcc ttatcagttc
     5761 gttgatgtca gtggttttga ccacgaggga acttcacgcg agttattgaa aaccctgaac
     5821 ccgttgtgtc agattccgac actggcgctg gaaaatgacg agattatgac ggagacagcg
     5881 gcgattgcat tgatggttct cgatcgtcgc ccggaccttg cgccaccggt tgggcgcgcc
     5941 gaacgtcagc agtttcaacg gctattggtc tggctggttg ctaatgtcta tccaacattc
     6001 actttcgccg attaccctga gcgttgggct cctgacgcac ctgaacagtt aaagaagaat
     6061 gttattgaat atcgtaaatc gctttatatc tggctgaatt cacaattgac cgctgaacct
     6121 tatgcgtttg gtgagcaact aacgctggtg gactgttatc tttgcactat gcgcacatgg
     6181 gggtctgggc atgaatggtt tcaggataat gccacgaata tcagtgcgat tgctgatgct
     6241 gtatgtcagt taccgaaatt acaagaagta ttaaaaagga atgaaattat ttgagtggga
     6301 taccagagga ttatgttatt tatta
//
LOCUS       DAETQN010000192         6305 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.194, whole genome shotgun sequence.
ACCESSION   DAETQN010000192 DAETQN010000000
VERSION     DAETQN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.194"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(336..4193)
                     /gene="sigA"
                     /locus_tag="K0W68_004099"
     CDS             complement(336..4193)
                     /gene="sigA"
                     /locus_tag="K0W68_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HBI3075513.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4554..5558)
                     /locus_tag="K0W68_004100"
                     /pseudo
     CDS             complement(4554..5558)
                     /locus_tag="K0W68_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5543..5920
                     /locus_tag="K0W68_004101"
                     /pseudo
     CDS             5543..5920
                     /locus_tag="K0W68_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5959..>6305)
                     /locus_tag="K0W68_004102"
     CDS             complement(5959..>6305)
                     /locus_tag="K0W68_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3075514.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1572 a         1408 c         1411 g         1914 t
ORIGIN      
        1 gggagacaat gctgtattct tttttaaagc actcaccaga aacggtgatt gtaatgataa
       61 tggtcttctc gatcgagatg taaatacccg tttcgttata ctgtccgtat cggaaccatg
      121 agaactcagt tgcattccag ccatgctcca gaatatgtgc atttttttgc ctgatagttt
      181 ctttttttat tcgctcatgt ttaaggcata acaggcagat aaataatgtc agaaaaagaa
      241 agctaataat gagagcaaga gcgatatagt ctgtcacagg gtaatacata ttctctacgg
      301 tagaagaagg gccgcaaacg cggcccgggc tgttatcaga aagagtaacg gaagttggcg
      361 ttgatcgcgt tatccacgtt gtatttacca aatgccgatt tctcaaactc aagaccgaag
      421 cgaagattat cgcgaatttc ggcgttgaga ccaacattca tgagaatacg accgtctttt
      481 tcacctttga tacgtttctc accggacgca tcacgcagta cggtttcacc gttggcaaac
      541 aggtcaaact ggtagccaag gccggcgcgg gctgtgactt tccagtcctt accggagaag
      601 gatttaccca catcaacacc ggtacgccca atcagcggat taaagtcctt atccttcatg
      661 gtcacgctca ttccgcgatc ctgccagtcg aaccgtttac ctgatacggc cccataaacc
      721 agttctgcct gcggctcaat ccaggagtct tctgttacat gatagcggta gcctgcttca
      781 gcacccacat acaaggaatg agaactgtag tcttttgttc cgagtccagc aaaggtcgcc
      841 gagtactcat tatcatggtg aacatactta ctaatcaggt cgatataggc accagagtca
      901 aatatagcgg aagcatacag acctgccccg acggacttcg tcttgccact gaatgcatta
      961 ctgctggcat gactgtcggt atacgtcata gtcagaccag tgaaaaggtc aagtccatcc
     1021 agctcatgtt ttttatccac accaatctgc acatgggtgt agttgtcact gtatccacta
     1081 gaagctgaac ctgctccgct catgatgcgg gcccatgcac cggcctcgcc attggtgtca
     1141 cgcagatccc ccattcgttt attcaggttg ttgacttctg tcaggaaggc tttgtaatta
     1201 ccggacatat acgattttgc tgcaccggaa gatgctgtat tttcagccac cttatatccg
     1261 gtcagggtcc acgtggtatg ttcctgatcc tctttcgtgc tgatcacagg agatatgttg
     1321 ctgaaaccta tggcctgagt actggcttta aatatctctt tattgcttcc ttttggggct
     1381 gttatcaggt caacgtttaa tcctgagtta ttatttccca ctttctgtat atcgaccgac
     1441 agactgttat tgcttccggt taactgattt ttaacaatga gtttatccga ttgatttaca
     1501 gactggctca gatcagcccg cataatcaca gcagtattag tggcatccag tgtgtttact
     1561 gtcacagtat ggaaacccga attctctatg ttactaaaca acaaaagact gtcgcgggtt
     1621 tttaatgatt gcagggctga ctgtccgttt actctccata aggtgttata catgctggct
     1681 gaggcagcag gagcatcaat acctccggta taagaagtat caaagccacc cagcaaacct
     1741 gtagccagcg gtgtgtgtgt ggttttgtcc aggttatctt ctttatactg gtcaacacca
     1801 aacagaatag atgctttttt gtctgatttt atattaccat tgacccaggc ataattttga
     1861 gcctttaacg tggcaccatc ttctgtcata ttatatccat ctgacatata gattgccggg
     1921 ctgtatttat tattttctgt aggggtgcct gttaatgtca gggttccggc cagttcaata
     1981 gctttatcta catacatttt tccctgggcg gtaacagtag ctcctttgtt gataatcagg
     2041 tctgtattaa ttaaggataa tgcaccagtc atagtaacag ttgatttatc accaacagtt
     2101 attttgctgt tttgagcact cagactgccg ttgaaactac tttcgcctgc tgaatccccg
     2161 gagcgaagct gttgtctgaa tccaaaacct tcgccagtaa tgtttcttcc tgagaacatg
     2221 tcaatgtatg cagttttatt tcccagaaca atttgagagt ttttagcctc tatgtgtcct
     2281 tttacattag catcccggcc tatactcagg gttgcgtttt ctaaatttaa tttccggaaa
     2341 ttaaatttac gactttccca gtcgggctgg gaaaaagagg ctatctgatt gttggactta
     2401 tattccgtat tgtaacgttt atttacagtg ctttcctggt cctttatggc agcagaatag
     2461 tctttttgac ataaaaaagg aattggacag ttgttattgc cttctttaaa tatggcatgt
     2521 tctgtcgcat gtccttgtaa tgttaactgt gcattccgga cagagatatc gtttttgata
     2581 tcgactgagc catcaaagat cagattggca ttattgttat gttgctgggt agtattgata
     2641 atatgattta tgcttatatt acctgataca ttcccatggt acatatatgc atcagtatta
     2701 gtaagtgata atgttgattg cttcacgttt gagttcgtta cggttgttcc cgcatctgtt
     2761 gctgcgattt tctgaaatga ctggtcatag ccgtttagat ccagtgttcc gccatttctg
     2821 gtaaaataga tttcgccatt accgctttcg cttaaagcgt ctttggcatt tatttttacc
     2881 gtacctttac ctccggccat gtaaatttta ttgaaggctt tttcagcact aaggatgaca
     2941 gtcccattac ctattttaag gttatttccc tgtgctattt ttacatccag agtaccacta
     3001 cctattttat gcagattatc tccggcaacg cccttaacat tccattcaac ggtggtatta
     3061 ttatcaatat ctatcccggc acctttataa gacttatctt ttccgtacac tttatatgtc
     3121 tggttcttat cgaatataag cccgccaatg ccaagatcgg tatcctgctt gagcgttaat
     3181 tcccctcctc ctgagacaga caggtcctta tcttttataa tcgatgataa ctcttgtttt
     3241 ttaccatcca gctgcatagt gtttgtgaaa ttctttttta aattgtttac cagaatatta
     3301 ttaaataacg agtagtttgt ccaggctata ttggtactac tgagaaaacc aactgtactg
     3361 gtaacaccat ggaaaaccca tttttgttgt tctttatcat atagataaga accagaacca
     3421 ctatcgccaa aaattggtga gtttttaaaa ggggttctgt tggtataaat gtaactcttg
     3481 tttttccagt ccagattaaa aagacttgcg cttaacaagt cgggattata gttttgtcca
     3541 aacagatatt gtttgccgtt taatatcgtt gaggttgttc cggcacctgc tctgaagcca
     3601 attatttgtt ccttaccgtt gtagtttacg ccatatcttt tcatcgcatc ttcgggagaa
     3661 agtgagaagt cggcactctc cgtaacccct gttgtttcca cgacgaattt attcagacga
     3721 tgaaccgaaa agtcgccaga tgatactctg ttacttgctt tatatgctgt ctgatcccat
     3781 gactgtgttg ttattgcatg atgctgtgta ccgttatgag tcgcagtaac actgtacgca
     3841 cctccaattg atgttgttgc acctttgttg gaaacagcag aaaaatcagg tacaggtagt
     3901 tctggaaaat gaaatatctt tccatctttc atcattaatt gaacatttgt tgcacccggt
     3961 ctgaactctc ctctgttttg agcaaggtca agataatcgc gggtccatat attatttata
     4021 tccagcattg ctgcaaatgc tgtttcgcca taacttccat atattgagga taaaataata
     4081 ccgaggataa ctttctttct tgatgtaccg acactaactt tccgggtcag ttcagaaaca
     4141 gcaactaatc cacctgtaat atgactatat ttcagtgaat aaattttatt catacgtgaa
     4201 ctccgatatt tctgagttgt ggttatggga taacatttaa ttacaacggg gaactatcag
     4261 tcatggtacg taactgataa gggtataatg ataatcactg tcagttatat ttgtatgaca
     4321 gatgtaacaa aatgaaaaaa aagataagca caagtgattg attggtcaga tattgatcac
     4381 ttggtgtttg tattttgaac tttgcgatca aaatttgatc tttgttgttt ccggtcaatc
     4441 ctttacaggt tttctgtatt atccggggga gttaagtctg tcgatgtccg acagactttt
     4501 gatgatggaa acaacatgca gcgataatgt gcatgctgtt ttgcaggtgg aactcagaag
     4561 gtgaactgga actgtgcgac cgcaccgtaa gtgttgtccg tgccgatcag gcctgcaaca
     4621 tcaaggtgaa ccacatcaaa cggtgagatc cccaccccgg ccgtgaatgc actgccgtcg
     4681 ttaccggcca gattctgacg gtaaccggcg cgcagctgtg cccatttcca ggcattaaat
     4741 tctgcgccaa tggcggcaaa ctgacggttg ctgtcggagg tgaaaccgct ggccggggtc
     4801 agatccacat caaatgcggt ggtgaacatc gcgttgtgcc aggagacacc ggccgtcacc
     4861 tgcgggcgga tcctgaaggt ttccgtgata ccgttcactt cttttgtctc aatactacgg
     4921 gggatcaggt tctgtgcgac cagtcccagc gtccagttgt catccaggtc cgtactggca
     4981 ccgatgtcgg cgttgatgcc atttttcgtg ttgtggtagc ggtcgccttt gaaggcgctg
     5041 ctgtcataat ttctgaccag cacgttgtag ttgaacaggt cgacgcgctg gaatttgggg
     5101 gggaagccca gagaccattt ctgcccggcc gtttccagtt ccgtagccag agcaataccc
     5161 acatcagtga tcagtgccgc gcgggcaaag gcctgtgatg tcaggcggct tttgtccacc
     5221 tcctgcccgg tgctgtttgc cacctcttcc aggtaagtga ggtcagcatc agagacttta
     5281 ccgtcgacac tgacggtgcc gtgtgattta accatcagtg cggcagagag gcgatcaccg
     5341 ggaagggccg ccacagcaga aactcccagc tgtgcggcgg catgtgtgtt acggaagtcc
     5401 cggaggtgtt ctttcaggcg tgcggcagcc tcaggaacac cgtggttact ttcgatggcg
     5461 cggtcgaagg ccttccactc atcactgatc cggtcggcgt tgtcggtgat attatccgga
     5521 ttggagagct gcgcgccgac ggacggaagc aggatgctgg gctcatgcgc gacgcaaaat
     5581 ccacgatgta tatatcagta ctaaaagcgc gacggcggaa gaagctctga aactaatcgg
     5641 tgaactgtac gccatagagc acgatatacg cgggttaccg gtgtctgaac gcctggcggt
     5701 caggcaaatg cagagtagac cgctcctgac ttccctgtat aagctgatgc aggagaaaga
     5761 acacacgtta tcgaagaaat gtcgtctggg agatgcgttc cggtatatca ggaagcactg
     5821 ggctgcgttg tgcaacttct gtgatgatgg cctggctgag gtagataata atgcagcaga
     5881 aagagcgctt cgtgcagtct gtctcgggaa gaaaactttt tcgaggatca ctcattccgt
     5941 gtctgactgc ttcagggttt aattttgctt ctgctgactg aatgatgatt gggcagcaag
     6001 tgcaatatcc cacataaatc cagttaactc acgagccacc gccgtaatgg ctacttgtga
     6061 ttttttccct tttgctgtca gagttcggta acgagaacac aatcgttgtt gagctttcca
     6121 tgcaatatct ttggaatatt gtgttacaga gtccggtcgg taatatataa gccatgctcc
     6181 aacttttgca ggtgtacgat aagaccaagc ggcttcgtaa agcagacgtc tcagttcgct
     6241 gtttccaact tttgttattc ctctgggacg aatactgttt ccactggaat attcacctgg
     6301 cacca
//
LOCUS       DAETQN010000193         6257 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.195, whole genome shotgun sequence.
ACCESSION   DAETQN010000193 DAETQN010000000
VERSION     DAETQN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.195"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            123..953
                     /locus_tag="K0W68_004103"
     CDS             123..953
                     /locus_tag="K0W68_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HBI3075515.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1094..1867)
                     /gene="uxuR"
                     /locus_tag="K0W68_004104"
     CDS             complement(1094..1867)
                     /gene="uxuR"
                     /locus_tag="K0W68_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBI3075516.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2082..3542)
                     /locus_tag="K0W68_004105"
     CDS             complement(2082..3542)
                     /locus_tag="K0W68_004105"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBI3075517.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3623..4807)
                     /gene="uxuA"
                     /locus_tag="K0W68_004106"
     CDS             complement(3623..4807)
                     /gene="uxuA"
                     /locus_tag="K0W68_004106"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBI3075518.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5147..>6257
                     /gene="gntP"
                     /locus_tag="K0W68_004107"
     CDS             5147..>6257
                     /gene="gntP"
                     /locus_tag="K0W68_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBI3075519.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISA"
BASE COUNT         1480 a         1620 c         1588 g         1569 t
ORIGIN      
        1 cgtgtagagt tgatatcact acaacagaaa catgcatgtc atttgtttac ttctcttttt
       61 actacagaaa aatttatcta tttttctaaa catcagtgaa atactcataa aaggtaacta
      121 ttatgtctat gccattaagc aatgcccttc agagtcaaat tattacagat aatcacttcc
      181 ttcatcaccc aatagtcgac agtgagttta cacataaatt aaaatatgca cgcatggatt
      241 tggaaaacat ctatcttccg cctcttactc gtggcaataa ccataattac gatggaaaaa
      301 gcgctgtcga aattagaaaa cttgatatta gtaaagagtc ctggccattt aattatgtga
      361 caggagcatg cagagaatcc gatggtatta caacaacagg acgtatgctc tatcggaact
      421 tgaaaatcac ctcagcttta gacgaaatat atggtggcat atgtaaaaag gcgcatgctg
      481 ccacagagtt agcagaaggt ttacggctaa atttatttat gaagagccct ttcgatcctg
      541 ttgaggacta tacagttcat gaaataacat taggccctgg ttgcaatgtt cctggttatg
      601 ccggaaccac gataggatat atttcaaccc ttccagcttc tcaggcaaaa agatggacca
      661 atgagcagcc aaggattgat atctatattg atcagataat gacggtaaca ggggtagcaa
      721 attcttccgg attcgccctt gccgcattac ttaatgctaa tattgagttg ggtaatgacc
      781 caataattgg tatagaagcc tatcccggta ccgcggagat ccatgcaaaa atgggatata
      841 aagttatccc gggtgatgaa aatgcaccac tcaaacgtat gactctgcaa ccgtcatctc
      901 ttccagaact gtttgaatta aaaaatggtg agtggaatta tattggtaaa taactaccag
      961 gaagaatgag tactaacatt caggttatac agatagctaa acaataccct gcaatttaca
     1021 aagtaatact cattgcgact tcttaattta agaaagcgca aggaacattt acccttgcgc
     1081 ttattataat aagtcaggcg tcaactttat ccagcggcca tgaatcaaac agatagccat
     1141 caaaataaat atcgtcaacg ttcgagaatt ccagcagacg ttgcttaacg ttttccagat
     1201 gccgccacat tgccagcttc gccgctcggg catctttttt gattaacgcg gcgaggatct
     1261 gtttgtgatc gcccaaccac tctttgcgat acaggctgtc atccagatgg ctgtgcaact
     1321 ggatccacat tggattgttt tcccgccact gccaggactg acggaacagc tccaccagca
     1381 tgctgttatg cgttgcttcg gcaatggcga gatggaactg catatcacca ctttcgctgc
     1441 tgcccggcgc gctggaagcc agctcacgct cttccagttg caatgcctga cgcattttga
     1501 cgatatcttc gcgggtagcc tgcaaagcgg caaattccgc gatgttactt tccagtaact
     1561 gacgcgcctg taacagctca aaaggaccgg catcattgca gacgttagca tccggactgt
     1621 ctgtgttgtg gctgcctgag ctatcaagaa catagatacc ggcaccccgg cgcacttcca
     1681 ccagcccttt gatctccagc atgatcagcg cttcacgtac caccgtcctc gtgacatcaa
     1741 gcatttctgc aatttcacgc tccggcggca gccgttcgcc aggattgtat ggcgtcttta
     1801 tgatcagatc gcggatcatc gccccgactt cctggtaagg tctttgcgca gaggtggcag
     1861 atttcatggt gttgtgtccg ttaggtgcgg ttaatcaaat ttttgccgga tgcggcgtga
     1921 acgccttatc cggcctacaa cgggcaattt tacggcaccc gttaatctca aactatacca
     1981 ttagcactgg aagttggcac gtacaggtgg gcaaaaaaac gaaaactggt caaccaaata
     2041 tcagtttgat tgaccagtaa gtctgtaggg taatcagtta gttacagcgc agccacacac
     2101 tcgcgcgcac cgcgttcgca cagctgctga tatgccgccg tcaccgcgcc aacaaagtcg
     2161 gcattctgcg gcagatcatc ggcaaaaatg ccgctcaggc ccagcagcgc tttcacgcgg
     2221 tctgcgccct gatactgcgc gttaatcttc tggaactcgg ccagcatcgg gtcaaccacg
     2281 tcaatggcat taccctgttc atccacgccc tgggtgtagc gcatccagcc agccacgccc
     2341 agtgccaggt gacgccagct gccgccgttt tgcaggtgca gacgcaccgg atccagaaga
     2401 cgctgcggta acttctggct gccgtccatt gcaatctgcc aggtacggtg acgcagagac
     2461 gggttgctga aacgctcgat cagcagcgtc gcataggcgt tcaggtctgt accttccggc
     2521 atcgacagcg ttggcgcttg ttcctgcatc atcagggcaa aggccgcttt gcgataatcc
     2581 ggattagtca tggtgtcggc aatggtttca tagccgccga ggtaaccgag gtacgccagg
     2641 aaagagtggc tgccgttcag catacgcagt ttcatcattt cgaacggcac aacgtctgca
     2701 acgaactgtg cgcccacttt atcccagtcc ggacgaccat taacgaagtt atcttcaatc
     2761 acccactgac ggaacggttc gcaggcaatg gcgcacgggt cgtaaacacc cagctggtca
     2821 gcaatttcct gtaaggtttc tggcgtcgcc gccggaacga tgcggtcaac catggtgcac
     2881 gggaaggtga cgttctcttc aatccatgcc gccagctgcg gatcacgtgc ctgagccagc
     2941 cccagaaccg cgaccttcgc cacatgaccg ttttcacgca cgttgtcgca ggacatcacc
     3001 gtaaacgctt tcagcccttt ttcacgacgc agacgcaagg cttcgacgat gtaaccaatc
     3061 gcggacttcg gcgcagtcgg gttttccaga tcgtgcttga tcagcgggtt attgagatcc
     3121 agctgaccgc ttgccgcatc ggcgcaataa cctttctctg tgaccgtcag ggagacaatc
     3181 gccgtctgcg gacgcgccat cgcgttgaga ataccttcac aaccgtcgat ttccggatgc
     3241 agggcttctt tcatcgaacc gataattttc agctcggtgc tttctgcgcc tttttccgcc
     3301 acggtgtaca gcagttgctg ttttttcagg ttttcaatca gcacacggtc gttgcctggc
     3361 atcaggttaa cttcgcagat accccagtcg ctgtcggtac tttccagcag atgatgggtg
     3421 tacagcgcct ggtgcgcgcg gtgaaacgcc ccgcaaccga gatgcacaat gcgtgattcc
     3481 agacgagaat gatcccatga cgggcgggca accggcagat tgctgtcaac aatagtagtc
     3541 atcgtaaact ccagaatccg tcttcagaga gtgacggcaa ggaagaagga accggcacgg
     3601 cgcgcagcca tgccggtggg tatcaacggc taaagaaagc gcgctggatc gccagttcga
     3661 caccgcgaac ttcagccagg cctttcagac gaccaattgc ggagtaacct gggttggttt
     3721 tcttcttcag gtcgtccagc atctgatgac cgtggtccgg acgcatcggg atcaggtctt
     3781 ctttgccttc cgctttacga cggtgttctt cttcaacaat cgctttcacc acttcgtaca
     3841 tatcaacgtc accgttcagg tgcgccgctt cgtggaaggt tttcgggtta tcttcacgca
     3901 tggtggagcg cagatgggtg aagtaaatac gcggaccgaa ctgcttgatc atatcaacca
     3961 gatcgttgtc agcacgcacg ccgtaggaac ctgtgcacat ggtgaaaccg ttcgccatgc
     4021 tgtttacggt atcaaccatc cactgcatat cttcaatggt ggaaacaatg cgcggcaggc
     4081 cgaggatcgg gcgcggcgga tcgtccgggt gaacagccat acggacgcca acttcttcgg
     4141 caaccggaat aatcgctttc aggaagacgg caaagttttc gcgcagtttg gctttgtcga
     4201 tatctttata cagctccagg tgcttacgga actggtcgag ggtataccct tcttccgcgc
     4261 ccggcagacc ggcaatgatg ttacgggtca gacgcgcttt gtcttcgtcg ctcatagtgg
     4321 cgaagcgttc agcggcctga gcaatttctt cttcggtgta atccgcttcc gcgcctggac
     4381 gcttcaggat atgcatttcg aatgcagcga attcgatctg gtcgaagcgc agagctttgg
     4441 agccgtctgg cagcacgtat tcgaggtcag tacgggtcca gtcgagcacc ggcatgaagt
     4501 tgtagcacac ggtgcgaatg ccgcactgca ccaggttgcg cagagtctgc tgatagttag
     4561 cgatccactg ttcatagttg ccagtgtggg ttttgatatc ttcgtgaatt ggcacgcttt
     4621 ctacgacaga ccataccagg cctgcgtctt caacgatcgc cttgcgtttg aggatctctt
     4681 ctacggacca tacttcgccg ttcgggatat ggtgcagcgc ggtaaccacg ccagttgcgc
     4741 ccgcctgacg gacatcagct agagaaaccg gatcgtttgg gccgtaccag cgccaggtct
     4801 gttccatgat ttttcctctt tcacatagtg ggttccacag caaagagacg tggagccccg
     4861 gagttgttta ggtatccgga tgaacaacca gaccgcagcc gtggcgttgc atcggatgag
     4921 atggcgtata agttctacct tttggtaaga catgtcaacc cgaaatctga aattggttaa
     4981 ccacatcgca agaatttcac tctcacggtt gttgctgcaa attgcagcaa agggtaatcg
     5041 atggttattg atcagaggat gtgacattca tcgcaacaat ggttgaccaa tttacataac
     5101 atgtcgccca aaataacaac ggttcaacca ggtaaggtaa aacgttatgc atgtgcttaa
     5161 cattctctgg gtggtattcg gcattggcct gatgctggta ctgaatttga agttcaaaat
     5221 caattcaatg gtggctttgc tagtggcggc gctgtccgtc gggatgctgg cgggcatgga
     5281 tttgatgtcg ctgctgcaca caatgaaagc gggcttcggc aacacgctgg gggaactggc
     5341 tatcatcgtg gtgttcggtg cggtcatcgg taaattgatg gtcgactccg gcgcggcgca
     5401 ccagatagcg catacgctgc tggcgcgtct cggtctgcgc tatgtacagc tgtcggtaat
     5461 tatcatcggc ctgattttcg gtctggcgat gttctatgaa gtggccttta tcatgttagc
     5521 gccgctggtt attgtcattg ccgccgaagc taaaatcccg tttctgaaac tggcgatccc
     5581 ggcagtagca gctgccacta ccgcacattc actgttccca ccgcagccgg gtccggtggc
     5641 gctggtgaat gcttatggcg cggatatggg gatggtttat atctatggcg tactggtgac
     5701 gatcccaagt gtcatctgcg caggtctgat cctgccgaag ttcctcggca atcttgagcg
     5761 cccaacgcca tcattcctga aagcagatca accggtagat atgaataatc tgccctcttt
     5821 cggcgtttcg attctggtgc cgctgatccc ggcgatcatt atgatctcta ccaccatcgc
     5881 caatatctgg ctggtaaaag atacccctgc ctgggaagtg gttaacttta tcggttcctc
     5941 gccgattgca atgtttattg cgatggtggt tgcattcgta ctcttcggca ccgcgcgcgg
     6001 gcacgatatg cagtgggtga tgaacgcttt tgaaagcgcg gtgaagagca ttgcaatggt
     6061 gattctgatc atcggtgcgg gtggcgtgct gaagcagacc atcatcgaca ccggcattgg
     6121 tgacaccatc ggcatgttaa tgtcccacgg caatatctcg ccctacatca tggcatggct
     6181 gatcaccgta ctgattcgtc tggcgacggg tcagggtgtg gtttcagcga tgaccgccgc
     6241 cgggattatc agtgctg
//
LOCUS       DAETQN010000194         6215 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.196, whole genome shotgun sequence.
ACCESSION   DAETQN010000194 DAETQN010000000
VERSION     DAETQN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.196"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            245..367
                     /locus_tag="K0W68_004108"
                     /pseudo
     CDS             245..367
                     /locus_tag="K0W68_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(629..1195)
                     /gene="ipgB2"
                     /locus_tag="K0W68_004109"
     CDS             complement(629..1195)
                     /gene="ipgB2"
                     /locus_tag="K0W68_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HBI3075520.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSREIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(1478..1792)
                     /locus_tag="K0W68_004110"
     CDS             complement(1478..1792)
                     /locus_tag="K0W68_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075521.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(1870..1980)
                     /locus_tag="K0W68_004111"
                     /pseudo
     CDS             complement(1870..1980)
                     /locus_tag="K0W68_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2304..2981)
                     /gene="ospD1"
                     /locus_tag="K0W68_004112"
     CDS             complement(2304..2981)
                     /gene="ospD1"
                     /locus_tag="K0W68_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HBI3075522.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKSNIINVTNSAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(3352..3489)
                     /locus_tag="K0W68_004113"
                     /pseudo
     CDS             complement(3352..3489)
                     /locus_tag="K0W68_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3568..3774)
                     /locus_tag="K0W68_004114"
     CDS             complement(3568..3774)
                     /locus_tag="K0W68_004114"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3075523.1"
                     /translation="MDAENETVLNANMTHHLGCEKNQLRSGSNSRNGCLTKIITTGDE
                     PLEIRTLRDRNGTFEPQQLKKNQP"
     gene            complement(4458..4617)
                     /locus_tag="K0W68_004115"
                     /pseudo
     CDS             complement(4458..4617)
                     /locus_tag="K0W68_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4621..5775)
                     /locus_tag="K0W68_004116"
                     /pseudo
     CDS             complement(4621..5775)
                     /locus_tag="K0W68_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(5795..6142)
                     /gene="tnpB"
                     /locus_tag="K0W68_004117"
     CDS             complement(5795..6142)
                     /gene="tnpB"
                     /locus_tag="K0W68_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBI3075524.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(6142..>6215)
                     /locus_tag="K0W68_004118"
     CDS             complement(6142..>6215)
                     /locus_tag="K0W68_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBI3075525.1"
                     /translation="RLKGKLTPALLQILIREIKGSSH"
BASE COUNT         1599 a         1408 c         1322 g         1886 t
ORIGIN      
        1 aaattcaggc gaatgctgtt tacgtgtttt tttgctggtt gatactgttt ttgtcatgtg
       61 agtcacctct gactgagagt ttactcactt agccgcgtgt ccactattgc tgggtaagat
      121 caatttgctc actggtattt actacgtgca tcgaatgagt gatcaagagc cgtatgacgc
      181 gagagtgtca cgtacggttc cgtgagcagc tggagagtga aactctctcc ggctgactcg
      241 actcgcaggt tccgacagcg gtgacgaaca tgcagcggtg ttgttctcgc tgatcggcac
      301 acgccgtctg aacaatgtgg agccagaaaa atggctgcgt tacgttattg aacatatcca
      361 ggactgaccg gcaaaccggg tacgcgatct gttgccttgg aaagttgatc tgacttctca
      421 gtaaatatca atatgattct gacgagccgc ttacctttga gcagagaaag gggagaggga
      481 tcccacatcc atgagctagg aaaatacaaa aactggctat acaacaattc aatatattag
      541 gacttacttg cgaggagaaa tatagcaccg ctcaggcaat tcctgggtat ttacttttct
      601 tagtttcttt actcgggatg ttctgtcatc agaaaggcga ttctaaattt gtaatatagt
      661 cactcatatt cccacgtaat ttctttagat caatatattg cacatttttc tcaaaaaact
      721 gatcggaaat aagatttgca acattgcttg ttgtatcatt tttaacgctt ctattcatcc
      781 cttcacaaag ttcgtccatt ttctttttga aataactgct tcctgataca agatgattta
      841 tggaactttg tgcagcattg atgtctagag aaatgcgata atctttctca acctgtttaa
      901 atatctgttt acggaggtta tcgccgattt ctcggctgtg ttctgcacaa aacttatcaa
      961 tacattggtt taccacccgg gatacgtaga cttttttttc atcatttttc caatctgtaa
     1021 tattttcacc gatttgttct gaaacagagc tcaaaatact agtatttatt ttggttgatg
     1081 atatttttac tatccttttc cccattgtac agcggataat ctcatccacc tttcctgaaa
     1141 aatatctttt gtgagataaa ctgattccaa aattattaaa agatgttcca agcattataa
     1201 tcactttgtc atgtaaaaat agtaacattg tcattatttt agctgattca aacaattctg
     1261 taaatgcttt gagttcttta ctcccttggc tcaatgatgg accgtctgga atcccagaga
     1321 ctaaactctc tgagaaagag agtatgaaaa aacttggggc agtccctaaa tgaagggacc
     1381 aacagcggta aaataaccgg catcattcat aatgatgccc tgttagttgt agttatgaag
     1441 atttacgtca ttaaaattgc tgttcgtagt gtcagtccta tggtctggca tcagctgagg
     1501 atcgtagccg atacatcact ggcttcacga cattcgtgtt gaggctattc atgaaaactc
     1561 cacgctgaaa acgccgtttt gtataaacgg tcacggtatg cccggagcca ctgttgctga
     1621 tgaattcgat aaaaccttgg cgtttcttga agctatcgtt aatgcggata atgaaacaac
     1681 gattggtgag attcgctctt ttgccgacgc cctggacgct gtccggttca atcgcaacaa
     1741 aatcaaccgg cagctgagca aacccaacct tgcctctctg gcgctggagc atgaagtgat
     1801 ttggctggga cgtagccgct gacagcttct ataaaagtat gatcctaccc acgtaatatg
     1861 aacacaggct taagtgaggt tctggttttc aaattgttca ggactgagac ctccacacca
     1921 actgtgccgt cgtcagcgat tgtaatcgca cttgatataa ttaaacactg tatatttcat
     1981 ttgtggagtc tccagtctga gggggcttta tgcattccgt attgggcact atgatgccag
     2041 aaatctgcga ggctccacac cttcctgtat ttcccttcct ctatcacagt gcactttact
     2101 taatagggtg ggatgtgttc attacattta ttgtgttttt tatgattgtt tttgcgtcca
     2161 gtgtcgttcg tcatgggaaa ctggttctat gggtagtatc gctcagtcta gttaactgtt
     2221 tgtttctttt tagaaattaa gcagatcaca caattcgggg agagatcctt ctaagtgcaa
     2281 ttctttgcag ctatttagca cacttaaaac aactcttgta ttttgtcaga aataatatta
     2341 aacatgtctt gttcattatt gttaagtctg tctgctattt ctgtaagttt tatcccacta
     2401 tgatggcata tatttatata tgccatcata gcctcgatat tccctttccc tgcggccaga
     2461 aataatcctg gtaaaccctc attatctctt acatccaata gtttaatcgt ttcttttggt
     2521 gataaatcaa aagttttaat aaaatctcca taagcttgaa tcgcagcgga atgaccttct
     2581 tgaagagcta tgaacaaagc agattcccca gcattatttc gtgctgcaag aacttctagt
     2641 ttttcctctt tgggtatttc ggctgccaac agcgattcac cgtatttacg cgctgcatcg
     2701 gcatttccat gccaaatcaa tgaaaacaaa ttatggcggt tatactcctt attattcttt
     2761 gtcggaatgg atatgctctg tattttggcc aattttctga aagaaacacc actatatccc
     2821 ccccctgatt tttcttcatt gatggcgtgg gatatagcgg aatttgtcac gttaatgata
     2881 ttacttttca ttcctttatt ttcattagca gtattgctgc ctattattag gtgcatattt
     2941 ttgttgtttg ctggatgtaa tccatagtta tttattgaca tttttgttca tcctttttgc
     3001 taaaaaaaac aataacctat ttttgtaatg taatagatat tagtctattg ccaatctaaa
     3061 aaaaccattg ccacattgaa tggctatatt caccaataaa ttctttcgat ttcgtatatt
     3121 gacatatcgt agatatatct tatccctaag attcaataat cgagtccgtt taccattctg
     3181 tgcaagcgcc ttcactcaga agtgaccgtc aaggcgatca ccaaacttga taataaagca
     3241 gctcattgcc atacgccagt ccttaagggg cgttgtccat ttctgtgatg ctgcctagat
     3301 ttaactgcca gctccggctt atctacatct aaatatgtga gcctaatacc gctacacttt
     3361 ggccagacta tgtttttctc cgggaagtgg gctaaccgtc tccataaagt gacgaacttt
     3421 tttcaacagt ggccatattg aacggcactg atgattacgg gttattgtct catgaagagc
     3481 cggccacatc actgacagca gtgacttcag gccttcagca tcagacagcc tgaaccagtt
     3541 aatccatcgc ccgacagatt tagtaatcta gggctggttt ttcttcagct gttgcggttc
     3601 gaaagtgcca ttacgatcac gcagagtacg gatttccaga ggttcatctc cagtagtgat
     3661 aatcttcgtt aagcaaccat tgcgggaatt gctgccagat ctaagctgat ttttctcgca
     3721 cccaagatga tgagtcatat tagcattgag tacggtttca ttttcagcat ccaatcaaac
     3781 tgactgagat cttcaggatt tttgagactt ttggctagtt cattagctaa agcccgcaac
     3841 ttattagatg tcaactgttt ttcattcata aattcacctt catttgatat caaattaaat
     3901 agttcaacat cagagaatta tacaaactta tgtacaggct cctatgatct tcgaatcctg
     3961 aagattatag tgaaaataag ttttcacgtt gaaatcccac atagttagta tatgataaat
     4021 aatcatggat gacctgatta acctcttggg gtagtgcttt tatagaagaa taaatattct
     4081 actaatttaa tacatcacct aaaatagtat tgttttttat ggttggttga aggtcgtggt
     4141 gatgatgtta gtccttttga gaatattcca gtgccagatc ttacttataa taaaggggct
     4201 tggcagtttg gataaactat tgttcttatt ccactaatac aaactttttg taaagtatat
     4261 taaatatact tcatttcata ctgaaatccc cggaagtatt caatcaaaca attacaccat
     4321 aaaaggaaga gggggcccta attaagttgt caagcatgtt tatgatacct gcagggtata
     4381 aattttctgg ctgcaagcag acacatataa aacgtcttta ttgatcctac ccacataata
     4441 tggacacagt cgtaagtgag gctctggttt tcaaattgtt ccggactgag accaccacac
     4501 caacggtgcc gtcgccagcg attgtaatcg cactcgatat aactaaacac tgttgcccgc
     4561 actatttccc gatggataat agtgttctcc gtggagacat tccattttca gcgaatgaat
     4621 tttcatctgt tgctgtatcc agtcatacag tgactgcatc agtggcgcgg ctctggcttt
     4681 tcttgccgcc agacgctgtt ctgctgaaca tccccggact tctgcctcga tggcatacag
     4741 ttcaccgata cgctgctggg cttccgttat tctgccggat tcgtacaacg cccggtaact
     4801 accgtaagca tcggcctgaa gcactccgct gtaaccggcc agatgattct gtggatggat
     4861 acctttccgg tccggtgagt acgcgaacca gaccgccgga ggcatttccg aaccggcgtt
     4921 acggtcatca cgaacgtaga cccacagccg ggcagtcctt gttttaccgc tgcccggctc
     4981 ctggaccgga acggggatat catcggcatg gactttaccg ggcatcagca catactggcg
     5041 caggatgtca tacagcggtt ccagcagttc agcaacagca cctgtccagc gccccagtgt
     5101 ggcacggctc agctccacgc cctgacgacg gtatatttct gactggcggt ataacggcag
     5161 atagtctgca tattttccag cgacaacatg ggccagaagc cccgctccgg cataactgcg
     5221 tgcaatgggt tttgaaggca ccggtgcctg cacgatatgg tcacaccggc aacaggccag
     5281 tttcggacgt tgtgtttcga taaccttaaa ggcgctgctg ataagctcca gctgctctaa
     5341 cacatcacat cccagagaac tgagttcgcc accacaggcc gggcagcatt cctcttccgg
     5401 acggatgacc cgggtttcac ggggaagtga ggcaggtaac ggtttatggg ctgaagactg
     5461 gcgcagggga gatggcagta ccgggtcata ttgctcaccc agcgtttccg ccatttcttc
     5521 ctgaagtgcg ctgattcgct cctgtgcttc ctgtatctgc cgttcggttt ttgcgcgaag
     5581 tttttctgag cttttaccga actgcatgcg ctgcagtttc gcaaccagcg ccttcagccg
     5641 gttgatttcg gaagcataag ccgccacccg ctgtgagagc aggcggttgt atttcgccat
     5701 ctggcggatg gtgtcctgtt gcgtctgcaa cagtgcccgc aggcgggcat tctcatgagc
     5761 aagtgaggtg tccatatcct cactttacaa cgggttatat gcggatgcca gcgcgttccg
     5821 ttcgtttcgg gtgtttccag tcgatgccct caagaagcat ggataactgg gccggagtaa
     5881 ggtgcacctt gccgtcacgg gtgaccggcc agacgaagcg gccccgctcc aggcgtctgg
     5941 taaagaggca cagtccgtca ctgtcagccc acaacacttt tatctggtca ccccggcgtc
     6001 cgcggaagat gaacagatgc ccggagaacg ggtcatcctt caggacgttc tgaacttttg
     6061 aggccaggcc gttaaagcca tttcgcatat cggtgatacc tgcaaccagc cagatacgcg
     6121 aacctgcagg gaaagatatc atcagtggct gctccctttt atttcgcgga taagtatctg
     6181 taataacgcc ggagtcagtt tacctttaag cctga
//
LOCUS       DAETQN010000195         6201 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.197, whole genome shotgun sequence.
ACCESSION   DAETQN010000195 DAETQN010000000
VERSION     DAETQN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.197"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            12..754
                     /locus_tag="K0W68_004119"
                     /pseudo
     CDS             12..754
                     /locus_tag="K0W68_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(796..1248)
                     /locus_tag="K0W68_004120"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="K0W68_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1312..1458
                     /locus_tag="K0W68_004121"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="K0W68_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="K0W68_004122"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="K0W68_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HBI3075526.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="K0W68_004123"
     tRNA            complement(2777..2851)
                     /locus_tag="K0W68_004123"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="K0W68_004124"
     CDS             complement(2927..3859)
                     /locus_tag="K0W68_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBI3075527.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..4908
                     /gene="mlaA"
                     /locus_tag="K0W68_004125"
     CDS             4153..4908
                     /gene="mlaA"
                     /locus_tag="K0W68_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBI3075528.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(5090..5425)
                     /locus_tag="K0W68_004126"
     CDS             complement(5090..5425)
                     /locus_tag="K0W68_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075529.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISNLFANKVDVDHPENKYKDFKDLEDNVEKRLQNRQAKYQNEINQLS
                     APGVNFDDI"
     gene            complement(5383..5775)
                     /locus_tag="K0W68_004127"
     CDS             complement(5383..5775)
                     /locus_tag="K0W68_004127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075530.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGNSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAMLQRGLKAVKLSWQNHLQQVPIIRL"
     gene            complement(5782..6158)
                     /locus_tag="K0W68_004128"
                     /pseudo
     CDS             complement(5782..6158)
                     /locus_tag="K0W68_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937824.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1546 a         1368 c         1374 g         1913 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 tatcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgctcgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt ctgaataaga aacaaataaa
     4921 aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca caagtaaaag
     4981 gagataaacc gacaatgacc cggacgtaat gatgtaaaaa tactgatgtt cgtcactgac
     5041 tatttccgat attcaggcgt tgaaaaatca aaaaactgga aaaataattt taaatatcat
     5101 caaaattaac acctggcgca gatagttgat taatttcatt ttgatatttc gcctggcgat
     5161 tttgaagtct tttttcaacg ttatcttcga gatccttaaa gtctttgtat ttattttcag
     5221 gatggtcaac gtcaactttg ttagcgaata aattagaaat tatttctttt acattgtcgc
     5281 gtctaaattt tgcatcgtcc acttgaaaat tatatcttat tgctcgacct aaacgtttaa
     5341 aaacttcgtt aggttctctc agtctttccc cgttgagctc gtttacaacc tgatgatcgg
     5401 aacctgttgc aaatgattct gccatgatag ctttactgct ttcaaaccac gctgaagcat
     5461 tgctgaagca ttcctggctt tttcactaca atattgggtg agactatcac cattaatacg
     5521 aattcctctt tgaatgtggt agtcgtcctg atcgtagtaa ccgagagata agtaatcatt
     5581 tccgttttta tttttaatta caatagcgag agagttgcct tttttatctt taccgtacca
     5641 ggtaaataag tccgttggtt cgctattccc gctctcatca aattctgtta ccgtatgtct
     5701 gagtttgtgt gatatcttat cgaagataaa accaggatct ccatcatata tttcttttaa
     5761 tgcgctgaag atcatttcct tgttattgat atcatgagtc ttctcaacta tgttgtcatt
     5821 aatgatagta cggacgagtt ctttgtcttt atagttcgca ggtaattcta ttggttcttg
     5881 gttgtcgata ccaatagtaa tatggtctgt atcattggag ccaatatcaa tataagtaac
     5941 tgaggaatta ttaatattaa atcttaagga tcgtcgttta ctcgtatggc gttttttata
     6001 agctcaagta gatgtttaat acaggtgctt attccttcct taccattaaa aaggtttttg
     6061 agctcttgta ctaatccatt aacttcatca ttgttatttt cattaatgga ttctgggtaa
     6121 gttgcgctgg tattgttgat tgaaatagat ggtagcatga ttttattcca ttttgattga
     6181 tttgttacaa atatatatta c
//
LOCUS       DAETQN010000196         6120 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.198, whole genome shotgun sequence.
ACCESSION   DAETQN010000196 DAETQN010000000
VERSION     DAETQN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.198"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..358
                     /locus_tag="K0W68_004129"
     CDS             <1..358
                     /locus_tag="K0W68_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3075531.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(398..1069)
                     /locus_tag="K0W68_004130"
     CDS             complement(398..1069)
                     /locus_tag="K0W68_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HBI3075532.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="K0W68_004131"
     CDS             complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="K0W68_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBI3075533.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="K0W68_004132"
     CDS             complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="K0W68_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBI3075534.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2798..3211
                     /locus_tag="K0W68_004133"
     CDS             2798..3211
                     /locus_tag="K0W68_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI3075535.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="K0W68_004134"
     CDS             complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="K0W68_004134"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBI3075536.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="K0W68_004135"
     CDS             complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="K0W68_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBI3075537.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="K0W68_004136"
     CDS             complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="K0W68_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBI3075538.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5600..>6120)
                     /gene="fliD"
                     /locus_tag="K0W68_004137"
     CDS             complement(5600..>6120)
                     /gene="fliD"
                     /locus_tag="K0W68_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBI3075539.1"
                     /translation="KEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAFK
                     TLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKGY
                     LADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTST
                     YLSQQFTAMSNS"
BASE COUNT         1497 a         1608 c         1527 g         1488 t
ORIGIN      
        1 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
       61 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
      121 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      181 ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt
      241 aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
      301 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      361 gtagcaggga tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact
      421 ccgcgccctg gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt
      481 ttgctggcac acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc
      541 gcatccattc ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta
      601 ccagaccctg gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct
      661 caaatggtgt gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa
      721 aaaaaggctg ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga
      781 ttgcccgacc atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc
      841 gggcgttaat cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat
      901 ccagatgaag gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt
      961 tgtatctgcc aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa
     1021 gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct
     1081 gagatcatac tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca
     1141 atgttcccgc tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg
     1201 gtcggcccgt cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga
     1261 atattattga tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac
     1321 tgcggcatcg cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca
     1381 aggcggtaat caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac
     1441 ggcggaagaa gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat
     1501 atgtcaccag caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt
     1561 cggtggccag cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca
     1621 cattgcccag accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc
     1681 cggtttcgca gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg
     1741 cgtttggtcc cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc
     1801 cgatggcact caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga
     1861 tccacatatc gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa
     1921 agccgacgcc aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg
     1981 cccagcccag catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat
     2041 ccggtttttg cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga
     2101 agatgggcag cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa
     2161 agaaccaggc atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc
     2221 ccattgccag acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc
     2281 ggatacggct gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc
     2341 agccgccaaa catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca
     2401 gatggataat tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct
     2461 gaccgcccca acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat
     2521 agtaagtgga gagaataccc gccgcgatga ccgcagggat gggtgcccag aatttaatca
     2581 accaggcgtg tttgaattgc tgccatgaca tgaatgtagc ctctgaactc agaaggtttg
     2641 aaacaagaat cgcgaatcat acatcatgca aatttatttt cggggtaata attcacaaaa
     2701 ataatagtta gatcaaattt ctctctgaac ctggaaaggc tggcgacatc agggctttaa
     2761 tgccacaatc ctcttttctt ctcatgcagg acgaatgatg aaaaaactcg caattgcagg
     2821 tgcgctcatg ttgctggcgg gctgtgccga agtcgaaaac tataacaatg tcgtgaaaac
     2881 ccccgcgccg gactggctgg cgggctactg gcagaccaaa gggccgcaac gtgcgctggt
     2941 cagtccggaa gagataggca gcctgattgt cactaaagag ggcgataccc tggattgccg
     3001 tcagtggcag cgggtgattg ccgtgccggg caagttgaca ttgatgtctg atgatctcac
     3061 taacgttacc gtaaaacgtg agctgtacga ggttgagcgt gacggtaaca ccattgagta
     3121 cgatggtatg acgatggagc gtgtggatcg cccgacggca gagtgtgccg ccgcgctgga
     3181 taaagcgccg ttaccgacgc cactgccgta atgaaatctc cccggcttgc cgccggggat
     3241 gaatttaaat cacctcttcg ataacccaca cgctgacgct gccgccgttg caaaagaagg
     3301 tcgcttcgcc gttttcgtcg gtcactacgc tctcttgccg attccccaga aaatcacgcc
     3361 aggttttatt gccgtaattc tctcccagat gaatggtttt ttcgccatca tccccgttcg
     3421 acatgaccac cacgcagccg ggatattcgt cagtgccact gcggctaaag gcaatgcagt
     3481 tcggatggtc gaaaaataac gtctgtacac cgtgggcgaa acgctgacgg gcgagaatta
     3541 actcatcaag ctgttcgatt attggcatat ctatcggata ggtttgcccg tcaccaccga
     3601 catcttcgta atgcgcaccg tagaggtccg gatagaacac cgaaggaacg ccattttccc
     3661 gcaacaaaat tagagcatac gccagcggtt taaaccacgg ttcgaccggc gcttcgaggg
     3721 cttgcaacgg ttgggtgtcg tgattggcta cgagggttac ggcgtggaaa ggatcggctt
     3781 ccactaatgt acccgtgaaa atctgcgtca tgtcgtagtc gcgtcccatg cgcgatgctt
     3841 catggaattt catctgcagc ggcgcatcga acagcatggt tttgccttcc acctgatcaa
     3901 tatacgtttg cagtttatcg acttcatgcg accagtactc tgccacaata aacagcggct
     3961 ttggcgcaac ttcctgtacg tgttcgatcc actctttata aaaccacgcc ggaatatgtt
     4021 taaccgcatc aagacgaaaa ccgtcgcatt gtgtttgttc catcacccag cgcgcccagt
     4081 atttaatctc ttccgtcacg gcatgattgc ggaaatcgat attctcaccc atcagataat
     4141 cgaaattacc gagttcatca tcaacctgat cgttccagcc ttcgccggtg tagtcgttaa
     4201 caattttaaa tatgccatct tcatcaggat tttcgatatg gtcgataccg ctaaaacatt
     4261 tgaagtccca gataaactgc gagtattgcc cggcacgggc ggggaaggtg taacgcgtcc
     4321 agccttcgca ctcaatgatt tcttcatcaa tttgcgtacg gtcatcagca tttacacgct
     4381 gcacgcgaat agcttctttt tcatccgccc ccattttgtg gttgactacc acgtccagta
     4441 gcaccgcaat gtcattacgt ttcagagcgt caatggcggc cagcagttgt gctttatcgc
     4501 catatttagt agggatgctg cctttctgat caaactcacc taaatcaaat aaatcatagg
     4561 agtcgtagcc gaccgaatac ccgcccgatg cgcctttata ggcgggcggc aaccagacca
     4621 tattgatacc aatatcatta aaaccgtcgg cgcgctcggc cagttcaggc cagagcttac
     4681 cgccttccgg gtaataccag tgaaaacatt gtaacagcgt gggattacgc attcttcgac
     4741 tccattcaag gggaacatta gaagcgtagc cgtaatcgga ttattcgcga gccatcggct
     4801 cgttaagatt caaaagaggt tatcctgcgg agccagcaca aagccgccct gatctccata
     4861 ggcacttaac actgattttt gcaccgatga ctgaccgacc agtttcgcca gttcatccat
     4921 acgaatctgt aataactgct ttaccttgct ttcgttgtcg agaatcaggc gcagcatcgg
     4981 gcggagctgc tcctgcatcg tggtggacgg ggcaacctct tcagtcagat gtgcaatctc
     5041 ctgcaccgca ttcacatacg ccatttcgct ggcgatgagt tcgtcccatt gttcttccgt
     5101 tgccaggcgt aacatgagct ggcttttatc gacgagttgt tgccaggcga aatacaaatg
     5161 cggtgcatgg ttcatcagac tgggtcctga atcaaagaag gggagagtaa agactctttc
     5221 caggcatcgg caatattgcg cattaatgct tccacttctt cgactgcgga gacatcgttg
     5281 cgtaaattgg cttgcagcaa gcgcctgacc atatagctat aaagagcaat caagttttgg
     5341 gttagttcgt ctttgctctc ttcatcaaga ctcacccgca gcccgttctc aatgatgttg
     5401 atcgcttttg acaaagagac gcctttgcct tgctgattgt tgtcctgcat aaacaggcgc
     5461 gctctaacca gtgcgctcag cactccatca aatagcatgg tgaccagctg ttgctggctg
     5521 gcgctcatta cggcgctttc gacgccaatt tgtgcatagg cttttgagcc gctcgcggta
     5581 tacatgctga cctccgtgat caggaattac tcattgcagt aaattgttgg ctgagatagg
     5641 tactggtgtt attcagtttg ctcatcatgg tatctaattg cgtaaactgt gcagtgtaac
     5701 gcgccacggt gtcatcaatg ctggcactga cggatagata ctgtttggtc agttttttca
     5761 gtgtggcgtt aatactgtcc tgtgcgctat caataatgcc atcatcggca aggtaacctt
     5821 ttacctcggt ggcaattttg gtggtaattc ctgtttcttt accatcgcct accagcaatt
     5881 cacgtacgga tgctgtattt tcattcagga cttttttgag tttatcatca tcaattttca
     5941 gcttacccgt cgtgccgtcc tgcttaatcc cgatctcgtt taatgttttg aaggcactgt
     6001 cgctggcacc gtttgcaaat tgtgcgcgga tgccgctttg aattgtacgt acaacgctgt
     6061 ctccgagaag tgcaccatta tttttatctt gtgcttcagc acctgggtct acttctttat
//
LOCUS       DAETQN010000197         6015 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.200, whole genome shotgun sequence.
ACCESSION   DAETQN010000197 DAETQN010000000
VERSION     DAETQN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.200"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(369..1304)
                     /locus_tag="K0W68_004138"
     CDS             complement(369..1304)
                     /locus_tag="K0W68_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBI3075540.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDLNAPAPNITIKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLS
                     TSARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTAETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
     gene            complement(1802..1945)
                     /locus_tag="K0W68_004139"
     CDS             complement(1802..1945)
                     /locus_tag="K0W68_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HBI3075541.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGV
                     ETQ"
     gene            complement(2029..2286)
                     /locus_tag="K0W68_004140"
     CDS             complement(2029..2286)
                     /locus_tag="K0W68_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="HBI3075542.1"
                     /translation="MGLKLHIHWFDKKNEEFKGGEYSKDFGDDGSVIERLGMPLKDNI
                     NNGWFDVEKRWVPILQPYFKNVIDISKFDYFVSFDYRDGDW"
     gene            complement(2296..3933)
                     /locus_tag="K0W68_004141"
     CDS             complement(2296..3933)
                     /locus_tag="K0W68_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HBI3075543.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENN
                     PWGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVS
                     GEALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTN
                     VQVSTLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASF
                     PGVPSLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKP
                     VYVSVTDVLTPAQVKQRQDEEKRLQQEWNDAHPVEVAERNYEQARAELNQANKDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQ
                     RAQTDVNNKKAAFDAAAKEKSAADAALSSAMESRKKKEDKKRSAENKLNEEKNKRRKG
                     TKDYGHDYHPAPKTEDIKGLGELKEGRPKTPKQGGGGKRARWYGDKGRKIYEWDSQHG
                     ELEGYRASDGQHLGSFDPKTGKQLKGPDPKRSIKKYL"
     gene            complement(4154..4687)
                     /locus_tag="K0W68_004142"
     CDS             complement(4154..4687)
                     /locus_tag="K0W68_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075544.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQRLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(4690..5616)
                     /locus_tag="K0W68_004143"
     CDS             complement(4690..5616)
                     /locus_tag="K0W68_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBI3075545.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTEPERVKSPSRGFSMGF"
     gene            5792..>6015
                     /locus_tag="K0W68_004144"
     CDS             5792..>6015
                     /locus_tag="K0W68_004144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075546.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEG"
BASE COUNT         1487 a         1660 c         1297 g         1571 t
ORIGIN      
        1 aaaaaaacaa cgacgagaac gcaatcaccg actctttctc gcggctgggc tactgatcga
       61 agcaggactg gtagacacaa aaacaggcga gctatgctat aaaaaagaca ggatattaca
      121 ggcacttaag gaaattaagt acgatctgga gacatcccca aacccagacg cgtaagtgcc
      181 tccatccgtg ttcctgttcc ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat
      241 gagactgcgg cgagcactac cagcacaatc atgcggaaaa cagcaccatt gcagggctga
      301 acggggaaaa acggcaaaag acctaaggct caaaaaaacg ccagaaaggc gctgttatct
      361 gatataggct attttccgct tttccagttt cgaatggttc gagtagaaac ctccaacatc
      421 gccgcaatag ctttctgaga atatccattc tccagcatac atagcgccat gaaacgcttg
      481 tcatcgtagg cttcgccttt agcgatgcca cctttcctgc ctctcttggc ctgtatttct
      541 ggcgtgtgcg tatcagccac atactgtgcg aaagtttccg ccgtgaagtt cctgtgcgtg
      601 tatttcgcaa tactcttgcc aatagcccga cattcaggag gtgaaagcgg aacgggaagc
      661 gatgcgttgt acatttcgac acgctgaatc acggcatcaa gccactgtga gaatgctggc
      721 cagccctgac gaatcgccct gtaagcccat ttgcgcgtca tttcgaacag atggcagttt
      781 ctccccattc cgtaatgttt atcgatgcta cggcgcgctg aagtgctcaa atcgagataa
      841 tcagccagtt catcgagggt ataggcttcc tcacgccact ccatcacctg ccattccaga
      901 tggaacggat ttttgcaaat cagtccgctg tagtttacat ctgcgcccag tttttcacac
      961 aacgcacgtt caatcgcggc ggcgtatttc agcgccttaa ccgaagcatc aggcgcggtt
     1021 ctcacggcga tattgagtgc gtagagcagg tgggcgtgac cgttcacggg attttttatg
     1081 gtgatgttcg gtgcgggggc gttcaggtcg ctccagtcaa tcgcagcccc ctcgcggtca
     1141 acgtcaaaaa ccagccagaa ctgcgcatga ggttggttct gctggatata ccgtgcaaga
     1201 agcgcgcgcc ctttaccgga gatacgaagg ccgaaagcga gatcatccgt gtgatagggg
     1261 cggaggggta aattttcttc gaagtattga agcgcggcgc tcatgtgtta cgattccttc
     1321 caggttatcg accgacctcg cccctgtatt ctcgtgacaa tcttggcgga gagacacgaa
     1381 tttcaggggc gtttgttttt cagtcgctta cactacaccc aacggcaaat cctgtcgatc
     1441 cttcccgaaa acccgatcca tttaccataa ctcccgttgt acgtacccct taaaacgccc
     1501 tcaatcgctt tctggcgcaa aaaacgcaaa aagacaagaa aacaccagga caaaccagaa
     1561 aaacgcgtca gaatcgtttt cagggcgttg cataactatg cacgaaagcg atgtgcgatc
     1621 ggtttcgtgc gttctgaaca ccgaacggct caaaacgaaa agttttctcc ctccggatgg
     1681 aaggttctgc acggagattt tagatcccat ctcccacatg gaatttttgt ggcacttcaa
     1741 caaaaaaagc gcgtcagaat cagcctgaca cgctttgtcc ttgtcaaatg aggattgcag
     1801 gttactgcgt ttccactccg gtcagttcag cagaggatga cggtgataca gtcccgccct
     1861 gaacatcacg gatatagttt gcctgacatg cagcaagaat aatggctgca agaagcaaca
     1921 aaataatccc tgttattttc ttcatacgat tccctgtata tatggattca ggaattcatc
     1981 cttgaacacc tgaatcaaac actttattac tcgtccctga tagtttaatt accagtcacc
     2041 atcacgataa tcgaaagaaa caaagtaatc gaacttacta atatcaataa catttttaaa
     2101 gtatggctgt aatattggca cccatctctt ttcaacatca aaccaaccgt tattaatatt
     2161 atcctttaaa ggcattccaa gacgttcaat aaccgaacca tcatcaccga aatcctttga
     2221 atactcacca cctttaaatt cctcattctt cttatcaaac caatgaatgt gtaatttaag
     2281 tcccataaca tcaccttaaa gatacttttt aatacttcgt ttcggatctg gacctttcaa
     2341 ctgcttaccg gttttagggt caaatgaacc aagatgctga ccatcactgg ctcgatagcc
     2401 ctcaagctca ccatgctgag agtcccactc ataaatttta cgccccttat ctccatacca
     2461 tctggcacgc ttacctccac caccttgctt tggagttttt ggcctcccct cttttaattc
     2521 ccccaaacct ttaatatcct cagttttagg agctggatga taatcatggc cataatcttt
     2581 agtgcctttt cggcgtttat ttttctcttc gtttaattta ttttcagcac tccttttctt
     2641 atcttctttc ttcttcctgc tttccatcgc agaactcaat gcagcatctg ctgctgactt
     2701 ctcttttgcg gcagcatcaa atgcagcctt cttattattt acatccgtct gtgcccgctg
     2761 ggccttaagc cctgccattt gccacattct gtgaccagca gccattggtt ctcgggcaaa
     2821 tcgctcgaat tgttttattt cagcctttgc atcagcaaga gttttattcg ctgcatcaag
     2881 ttcactttta cgagaattat aaacctgaac agctttagcc tgtcgttcct ggtttctggc
     2941 aacatcttta ttagcctgat tcagttctgc gcgggcctgt tcataattgc gttcagccac
     3001 ttccaccgga tgtgcgtcat tccattcctg ctggaggcgt ttttcctcat cctgacgttg
     3061 ttttacctgt gctggggtaa gaacatctgt cactgataca taaaccggct tctgcccact
     3121 ttctttcgga aagcgaatca ccgcttcatg tgaaccacca ccaaaggtaa atcccgcagg
     3181 aacggcagtc cggcctttat cctccgtaat accacgggga agggttgttg acaccggaag
     3241 gcctttgaca gtgctgactg tcagagaagg gacaccagga aatgatgcgt gaaatacacc
     3301 tggagtacgt gttggtttgg cattcacaac aggcacactc ataggcacac ctgcaactac
     3361 ggcaatatgc tgtcgtgtgt ccttcacaac atctgtcaca cgtttcgtca cgctgacagt
     3421 tgcctggtcc agcggaagtg tactgacctg aacattcgtc actgtttctg ccggtaatga
     3481 cgtcacaatc tttgacatca tattcgggtc atcttttgct atttcagatg gcagaatgcc
     3541 gtaaagcgca ataccccatg cgctgaattt aaacgggcct ttcagggctg caaagatatc
     3601 cgctattgcc gcagataaag cctcaccaga gacggcgata cccagcgttc cggcaccagg
     3661 agttgccaaa gcaggaaaac caaatgccac tgctgctacg gatgaattgc tgccaccacc
     3721 ggaattacca ttccccccgc cattaccatt gccggagcca cctccccagt gaacaccact
     3781 accggaaccg ccaccccatg ggttattttc cgaactccat cctgaaccgt cagaagcccc
     3841 accatttcca ccaagccccg tagggccgcc attaatgtta ccacctgtgt tatgtgcgcc
     3901 actgttgtgg tcgcgtccat ctccaccgct cataaaaatt ccctctttta agcgttaaaa
     3961 caattgatta tatagttgat actgtacata aaaccactgg ttttatgtac agaccaaaaa
     4021 gtactcgcca ttattttttc tgtcaagtca gggggggata ttttttggca gttcggagga
     4081 atcactcatg aaaacggtga acggaacgcg aaatcctttc gcgggtcgtg aggcgttgga
     4141 caagcgaagc gcgtcagttt ttccggctct tacccgtgac aggtttctcc accagtttat
     4201 cttccagtgt cacaactttc tgatacaccg ccgctgtggc gttgtagttc ctgtcgagaa
     4261 ttgttttcat cccccatccg agcacactga acataaccag aaaggccact gacaccccac
     4321 ccagccattt ccagctatac cctttaaggg tgttgtattc ctgcacgata ttgctgtgaa
     4381 ttgtcgcaat atccctgttc agggcatccg cgtttgcctt catgtttgcg actgactctt
     4441 ccagccgctg aaaatcctga ctgatgcgct cacctatcag ccctgcaaca tccagcctct
     4501 gcacttcgtt tctgacttca tccctgacct gttttgccac accgtcagaa atatcccgat
     4561 atccgcgaag tgtttccctt gccacagcca gagaatcagc aacagctttc tgttgctcca
     4621 ttgcggtatg ttgcagatgc tccaccgata acaggagcgt gagtaatttt tcttctgtgc
     4681 tggccatgtt cagaatccca tagaaaatcc acgtgacggt gatttaaccc gttcaggttc
     4741 agttcggttc tgtctgctca ctctgtctct catgtcaaag ccatgctccc gcgcaacatc
     4801 atgttgcagc cttctgacca gttcatgttg cttaaccgga gtgtccagcc attccctgat
     4861 gtcatgcccg accgttcgcg cctgttcaag atggcgggaa tagtccggtt cagaccgttt
     4921 ttgcatgatg tcatgacgct gggcatcatc aagccgtccg gcagcttcaa aacccagatg
     4981 cacttcggga cgtcgttcaa tgccctgttc tttcagcgtc cggcagtcaa tgaagcccct
     5041 ttcaaagcca tgctttctca tatgttcgtt gtgcttcacc tcccagcgtt ttcgcaattc
     5101 ccgcaactgc tctttttgct gagtgagagt caggcttccg gaatcctttt tcgcacctcc
     5161 ccgctcggga tacctggcgt tatagcgccg gaaatactgt tccggtgtac gttcaatgcc
     5221 atcattcaca cgctggctca tcatgatatg cgcatgaggc tgttcgccgc cgtcaatgct
     5281 cgctttcgga ttgtgaatgg caaaactcca ggcgtgccgt tcgccagctt cctgacgtac
     5341 aaaatcctgc accagttcaa ggcgctgttc cgctgtcagt tcacggggta atgcaatttc
     5401 aagttcccga taggtcgaac cgttcgcacg ttcaaactgg tcagccgcct gccagaaatg
     5461 tgagggatta tctcgcgccc attccggcat gtttccgtgt gcggaatatt ccaaatcctg
     5521 acggtcgcgg tatttctctt tgcgctcgat gtaatcggcg tggtttgcag cctgcccctt
     5581 gccaccaaat ttaacgctga ggtgataggt tgccacggtt tcagccctcc gcaggagcgc
     5641 actgtttctc gacgaaggag agaaacatat aagtgcgctt ccttctaatt ttacgaatta
     5701 gcacggaaga taccccctgc acccaccgac cgcaagcgat atactgcacg accacaacac
     5761 acagcaaaaa acaccaggga gcaccacgaa catgcgcatc agcaacatcg aatggctgaa
     5821 gaaacggatc ggattcatca ggaagctcgg agaacagacc gcacgacaga gacagattat
     5881 tgacctgctc gacaacgaag caggactcac agaacaggaa cgcaaactcc tccacgtact
     5941 ggcaacagcg gaaaaaaacg acttacaggc acaggagagc gaacgaaaac aggcggtcca
     6001 aaaacgaatc gaggg
//
LOCUS       DAETQN010000198         5963 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.201, whole genome shotgun sequence.
ACCESSION   DAETQN010000198 DAETQN010000000
VERSION     DAETQN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.201"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            294..370
                     /locus_tag="K0W68_004145"
     tRNA            294..370
                     /locus_tag="K0W68_004145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:328..330,aa:Arg,seq:tct)
     gene            complement(415..1176)
                     /gene="envY"
                     /locus_tag="K0W68_004146"
     CDS             complement(415..1176)
                     /gene="envY"
                     /locus_tag="K0W68_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBI3075547.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1359..2249)
                     /locus_tag="K0W68_004147"
     CDS             complement(1359..2249)
                     /locus_tag="K0W68_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBI3075548.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2250..5222)
                     /gene="nfrA"
                     /locus_tag="K0W68_004148"
     CDS             complement(2250..5222)
                     /gene="nfrA"
                     /locus_tag="K0W68_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HBI3075549.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5209..5940)
                     /gene="nfrB"
                     /locus_tag="K0W68_004149"
                     /pseudo
     CDS             complement(5209..5940)
                     /gene="nfrB"
                     /locus_tag="K0W68_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
BASE COUNT         1355 a         1565 c         1556 g         1487 t
ORIGIN      
        1 cattctgatg ataggatgag tatccataat gatcaccgac gttggtttca tagttaccag
       61 tctcatagga gcggacaatt ttccgttagg gcggtaaatt gtactttgat acatgaaaat
      121 acgggttttc ttgattcaaa cgcgcagcgg tgtgcatttg tttgccgcta tagcgtaata
      181 aatcagaaaa tcagacgcgg tcgttcactt gttcagcaac cagatcaaaa gccattgact
      241 cagcaagggt tgaccgtata attcacgcga ttacaccgca ttgcggtatc aacgcgccct
      301 tagctcagtt ggatagagca acgaccttct aagtcgtggg ccgcaggttc gaatcctgca
      361 gggcgcgcca tttcttcttc atttaacccc gtcttatccg ttccgctttg ctcttcacca
      421 catcactttt tgtcgctgtt tggcgagata attcaacggt gtcaggccgt aaaacgcctt
      481 aaaaacagag ataaagtacg acgtgctgct atagccacat aattgcgcca cctgagtgat
      541 atttttgtta tccatcaata acatctgtac ggcgtaacgc atacgacact ctgtgacaat
      601 ctggctatag ctgatatttt cgttttttaa tttctttttg agcaggctgg ggcttaaaca
      661 tagcgaactg gcgacaattc gcagattcca gtaatgctga atatcgcttt gaataatgcg
      721 gcagacgctg tcgcggacgc tgctgcgtaa gatatacatc agtagggcaa taaaccgcga
      781 ttgctcaaga aagttagaca atacggtaaa aagcaatgcg cgcgtcaact ccgcttcgca
      841 gggtttgctg ggttgctgct tgctgtggtt ggcggctagc cggaacactt ccggtgtacg
      901 gctatgacaa gcgataaccg gggttgccag ccgcgaccag ggctttacac aggttaagtt
      961 cttatttaaa aacaacagat aatcgttgat gatgttacgg ttgatgtggg tgattaatac
     1021 gtgatttaac gttgagagtt caatgacgtt attgttgcac gcgaaggcgg ccaggtagtt
     1081 tttcggaagg gtaaacgtag catggttatt gacgcttacc tctacctctt tttcggtcag
     1141 gatcaccacg caaggttcac tgctgctcaa ttgcatttcg cactcctcag atatcagaaa
     1201 ctccgctcaa aggatctatg cttcctgcat gagtgatcgg cccgttcgcc gataacgatc
     1261 ttctttcttt agcacgcttt ttagcaatta atcttgatgg aattctgacg agagcgaaag
     1321 aggtaagcca ggtcgtaccc gacttacctg gaggagattt aatactcgag aatgccgtgc
     1381 gcgacgggca aatagcgcag agagaaatag agagtgtctt taccgcaaga ggaacgtttc
     1441 gctaacagcg aggcgatctc tgcatccggt ttcggtttcg ctgtaaaggt tttgcctttg
     1501 ccggcgacaa acagttcata aacaatgccg cttgctgggg cggagctttg gcaatcggcg
     1561 ctggcctgta gataacgttc acgctgttca gcggtaagtt tatccacgcc gctgccatcc
     1621 tgtacccaga cattaactcc ggttgcttta acttgttcca gcagttggcg atagccatct
     1681 ggcgacatgt ttccggcgaa aaaactactg atataaaccg gttttgctga aacatcgcta
     1741 atcagccgct gcgcgttgtt taaccatgtt agcaaaggct gacgggcagc ttcgctgcgc
     1801 cagttcaggt cgtcaatttc cgcgctgatg taccagccat ccggcgttat gccaggcgcg
     1861 gcgctccata atctggcttg ctggagatcg gcagccagca ggcgattaag atagctttcc
     1921 agcgctgcgg acgactgttt ctggtgcata aaaaattccg gatcggcgtt cagcccgaca
     1981 atcagtttca ggccagcctg ttgcgcagct gcggcacgct taaacaataa cgtgcgctgt
     2041 tctggctggg taaatgcatc gccgtaacgg gtccattgca aaaccagggt atcgaagcct
     2101 tgcaaacgta actgactcat cagcccctgc cactgggtat cggtaacctg actatctcgg
     2161 ttttgtggtt gccagataat acctttcatc gcaaaggaaa aagggctgac caaaagcagt
     2221 gtcagcaata cgaaaatgaa cttacgcatt taccagtgca ctccaatggt gagaaacgcg
     2281 ttgttgcgct ctccgttacg ttgattaatc gccttaaagg tatgttgata ctcgacgccg
     2341 agactgactt tgtgcggcca ggcgtcgtag tgcgtctcgc cggtccagat attccagcgg
     2401 accccgactc cgccaagctg cgcgccctga gtgcctttat cacgatagcc gttgtcctga
     2461 acgtgagcgt aaggctcaat agtctgtccg ttagctacct tctgatgcca gctgacgcga
     2521 taatctgccg tccacgcctg aatatcctgg cggatatatt gcgccgcatc gaggtacagg
     2581 ttttgggcaa accagcctga accgttcggg tgccattcgt cgctgtattt gccgccatta
     2641 aagaatgagg cgctggcgcg cagcatggta tcggatgcgc cattttgccc gttcagcggc
     2701 aactgctgtt cgacggcgag gaaaaagatc tgatcgcgca gcggcttcca gcgcagaccg
     2761 gtgccggaca tcggattttt caccggcatc atcaccccgt tttctccggt atcggcaaag
     2821 acgcggctat aaactgagag caggtcgcct tccagcagca tattgcgtcc gatgcggtac
     2881 tcggcttcca gttgcccgta gctacgatag cttttccctg gcgctgcgcc gccaacatta
     2941 ttgttagcgg tactcattgc cccggaacgc aagccgatag aagaatcgaa actgaacgtc
     3001 cagcggcgac cgacctcctc atgcaaacgg cggaaattga agcgttgttg attttgctct
     3061 ggggtcagtg gggttatcag agcctgatta tcaatgtcat caatcaccag ccgggcgtag
     3121 tgctgcgtcg caggcatgtc atccagacgc tggttcacgt aggccagttg tcggatcagt
     3181 gccggatcgt ccggaagcct tttatgcgcc tgttcgagca tttcccgcga ctgtgcgata
     3241 tcaccgctat cccacaaggc gtaaccaagc gctgcctgga tgttgctatt attcggttcc
     3301 agttccagcg cggcgcgcaa atcactcacc gcggccggga cattatgacg ttggcgataa
     3361 attgtcgccc gcgcaacgta agcgttggca gaaggcgcaa tattgattga gcgcgtgaga
     3421 tcgttcagtg cgagttccgg ctgaccagga atgtaacgtt gtgcatgcag ccaccagtag
     3481 agggcattgc ttcccagtcc acgttgttct gcctgttgta gccagcgatc gcgagccgca
     3541 ccatttcctg ccgcctgggc ggtattggca gcagcaagca gatcctcatt gctcatgtcg
     3601 tgaagactga ttttctgcca ggccgccagt gcggtggcgt agtcctcaac ctgatacgcc
     3661 tgataggcta ccgcacgatg ttgccaggcg ctcggttgtc gttgttcggc ctgaagccat
     3721 gcatacaacg ccacaccggg tagcgtgtcc cgataacact ttgccagacg gttccaggcg
     3781 gcagcatcgt aggaaggcga catatcgccc agcaagcgaa ctattgccgg gcaattatct
     3841 gcaatacccg gcaactgact ttgccactga cgttgctccg ccagcggtaa gggtttcgat
     3901 aaaatcgcca ccttcgccgg cgttgccagg taaggatgac tttccagcag agacgccagt
     3961 cgcgccatta aagtctggct gacacgcgca tcgccctgga aaggatagcg ttgcagcaat
     4021 aaatcggcag cttcgcgtga ctgctcgttc tgcatcagtt gccaggtcag ttgatccagg
     4081 cgggtaagat ttgccggttc ttgctgatac agcaatcgtg ccagacgcag agattcagcc
     4141 ttgttacggg tcgccacgct gacagcataa cgctcctcaa gcatttcatt ggcggggagg
     4201 gtggcgagca gtttttgcgc tgcgtcgtac tgaccttctt ttaacagcac cggtagcgtc
     4261 gcgccaacaa catactggcg gttatcggca aactgcaccg tataattcgc caatgcctga
     4321 acggggttgg cgctgtattt agataacaga tagagccaac ttttctcttg tgcgtccgtg
     4381 gtaaacagcg gcttattttc attgagataa tgctggaggc gtgctttttc gccacgataa
     4441 gccagcgcgg tcgcgtaagt aatatatgac tgaggatcgg tgaagatccc ctgtgattgc
     4501 agtgctagga tccgatcgtc cagctgcccg gcaagaagca cgtcaaacca ctgacggcgt
     4561 tctgccgcgc ttaatgtgtt ctgctggcgt gcttcattgt atagcgtatc tgcctgggac
     4621 cattgtttca ggtagattgc ccgttgcagc agatcggttc gcagcgtttt tccttccggc
     4681 gatgcagcaa acgtcgcatc gttcagttgc gctctggcga caggtaactg tgcaagccgc
     4741 agggcattct gcccgacttc actgcgacaa cgcagggtcg gcgcagcatc gcacgctttt
     4801 tgctgggcaa gcagttcttc aacagttgta acgcttttca cttcaaccgg aatagccgcc
     4861 agactgcgct caagtcgggc atctcctggg tgacgtttca gttgatcctc aagcaacagc
     4921 cgcgcccggt catcatgacc aaaatggcga taggcttccg caaggtataa agtcagcgga
     4981 atattatccg gcacctgctg gtgtatatat tcaaattcgc ggatggcggt tgcttcgtcg
     5041 ttatttttct gtgccttcag cgccttatcg agacggggat aaataacaaa atggcgataa
     5101 tcgctcagcc ccagctcttc tgcgctggtg ccgatattgt ctgcgagtgc gctggtactc
     5161 aataaagacg tcagcagtaa accagaccat ccgatgacgc gattaaggtt attctccttc
     5221 attttcggac tccagttgcg caacctgttc tgtgtttaaa cctgctttga gtaatagtga
     5281 ttgcatcgaa acttgtaatt cgcgttgaat tgtcaggacg cgatccaacg tttcctggct
     5341 gataacgcct tcggtgacca aaaacttgcc gagcggcaga gaactgcgtt catggcgcaa
     5401 taacaacacg ttaattgctg aacgattaat atgaccgagc gtggtcagta tttcggcgaa
     5461 caggaactga tgcggcacat attgccgcca gatttcaccg gcctgctgtt ccgtgagcca
     5521 ctgatgctga accgcattgt acaacattgc ccgcggatcg tgaccgcgtc ggcgtgcata
     5581 ccagtgacgt aaccccgtga caatttgtcc ccgcagaaca atgaagtaac gcactttgcg
     5641 tccgacttta cgcgtcaggg ccgccagcga aaccgggtca ataccatctt cactgccgac
     5701 aattaactca tcattttcca gacgcagcgg cagtaccgca taatgcagcg ccacggaggc
     5761 cggcatttcg gcaatcagcg aggaagggat ctgccaggca tcgatggatt cccacgccac
     5821 gccgttttgc tctgccagcg cctgtgccag ctgctcggcg ctaatcagcc cctgcatcag
     5881 cattgaaccg cccaagcgta gaccttcgac gcgattacgc agtgctgtat cgagttgttc
     5941 ggtaatgact ccaacttatt gat
//
LOCUS       DAETQN010000199         5836 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.202, whole genome shotgun sequence.
ACCESSION   DAETQN010000199 DAETQN010000000
VERSION     DAETQN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.202"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..242)
                     /locus_tag="K0W68_004150"
     CDS             complement(<1..242)
                     /locus_tag="K0W68_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBI3075550.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(604..3387)
                     /locus_tag="K0W68_004151"
     CDS             complement(604..3387)
                     /locus_tag="K0W68_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBI3075551.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAELSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3444..5816)
                     /locus_tag="K0W68_004152"
     CDS             complement(3444..5816)
                     /locus_tag="K0W68_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBI3075552.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1489 a         1319 c         1311 g         1717 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatagc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaaataa caaaatccta atgttattta tcgtgagata
      361 ttacgagaat aatatttttt cattgaaaaa caatataata tgaaattctt gggtggtggt
      421 aaggtgtttt atgctgttat ttttatgcgc attctgtgcc tcctgaattt tcacgtaaaa
      481 atcagacctt aaaatatcac tattagtacc tggttattat tttgaacgca tttataaaat
      541 tattacataa aaatggcgaa tattgctaaa atccccgcca acgatgtgtt gacggggctg
      601 ttattatttt ggcaataata ctccagtata agtatttacc ggatgagaaa gatattgttt
      661 aacggcagtg ttaacattct ctaccgtcat ttgtttcaac aattgctcct gctcagtcca
      721 tgctgcagga tcgtcatatt gaataagact atttacaata gtgttcgcta attgttgaac
      781 gctacgctgt tggatatcga ggctgcgctg aacgttttgc tggtattcat tcagttcttg
      841 ctcactgatc cctttagcca gacgcttaac catcacttca ttcgctaacg ttaacagttc
      901 atcatgtcgt tctggttgac aagtaaaagc cagcaaatga ctgatatctt tggcctgagg
      961 atcaaccgag aggcgagaag aaacgctgta tgctccagat gcctgttcac gaatattaac
     1021 gcgtagatct tttgccagtg cgacgttaaa agcatcgagc gccatacgcg tcgccagatt
     1081 aacaggtgtc cgggaatcat aacgcttcca ctgtgaaacc tgtgccacag gttcattttg
     1141 ttcttttaca gtaaccgatg cgttgtccgt cgcgcgagtt aatggtttac ctgcggctaa
     1201 tggcgaatca gagtgtttga ttgatcctaa gtaacgcgta attaacgcca cgagtttgtc
     1261 ttctgagaca ttaccgacaa tgacaaacgt gatatccgct ggagatgaaa acaactggcg
     1321 atcggcagcc agcgcatctg cggcagtaaa ctgtacaatc tgattttctt gcagtaattt
     1381 cgtgcggtca tcagcatagc gcgtctcata catctgctgg gcgaatttct ccgctggacg
     1441 ctggtcgagc gttttcaacg cctgaatttg agcattttgt agcgatgccc aaatattgtc
     1501 gttaatcgtg ctgtgggtga ttcgctggtt aatcaactga aaaccaggtt cagggttatt
     1561 agtccgcgcg ctaacggaga gcaacgtatt catgccactg actttactgc tcatggttac
     1621 cgaattttcc gcactccagc gtttcaggct ggaagaggag agctcaccaa cgccgcttcc
     1681 gcttactgct ttatttgcca gcgcgataag tgatttttgc tgcgcaggga aacttaaatc
     1741 gcctttatta gatacggcag taatttgtag cttttgctct tcacccgcgg attttgccag
     1801 aataaccctg gcaccattgg aaagtgttaa tgatgtcaga ttctcagcca gcgtttcttt
     1861 gctgctaatt tccgcctgtg ggtcagcgtc tactgttaac gataaatttc tgcctgggaa
     1921 gatgtaagcc gccagctttt tgttggcgta ctctttttcc agcgccagga tagccgcaag
     1981 agacaatgct tttttggcgg caagctcatt gtttaccatt tgctcccaaa atgcgtcctg
     2041 gttctttctt aactgctgcc atttttccgc cagactttgc acggtaattt gctgccacag
     2101 acgtttcgaa agttgatatg tctcttccgg cgacaagaac ggcgtattat ttaatgagct
     2161 ggatgccagg cgactggtca gcatacgtaa atcacgttca gcttgctgat caaccgcatt
     2221 tttcagccag gtgaggcggg tagatttgac atcatcgagt tcttcagcag aaaaaccatg
     2281 ctgatcaatg gttgccaact ctgccattaa tgcatttgca gcatcctgca tattatcgtc
     2341 tcgtgcattt acacggaaaa acagcgactg ataatcgggt gcaattttaa cgctgcgcgc
     2401 agtgccgcca gaaatagtct tcaactcgcc cgactgtatg cgttcctgca gacgttgatt
     2461 gaacagctga actaacatgc tcaattcagc ttgttcgaca aagctttgct catcgttcac
     2521 ttgtaccatt ggcaggcgat aatagagtgc gatgccgttc actcggtttt ctttatcatt
     2581 aatgatatta aagcgcaggt ggttttcggc ttttgtcggc cagacgcgat tttcagctgc
     2641 tttgttagcc ggaagcttac ttaaattatc ctttatcagc gccagcgctt ctttactgtc
     2701 gatatcgccg accacgataa aggtcatatt atttggttga taccagcgtt gataaaattg
     2761 gcgcaattgt gccggtgtga ccgtggcgac agtatccatc aggccgatag gttcacggtc
     2821 taaattacgg gtatttgcca gcaggaaagg gcggcgcgcc tgagaggtgc gccatttcgc
     2881 atcctgatgg gcacgccatt cctcagtaat tacgccgcgt tcagcgtcta cttcgagtat
     2941 ttcaaaggtt gcggcattac tccattcact gaagattgcc atcacttgtt gcagattttg
     3001 tttctgcgta gtcggcagac tcacctgata cagcgtttcg tcatagctgg tataggcatt
     3061 aacatcgcga ccgaaacgca ggcccattga ctcaaatgtt tcgatgactt tattacccgg
     3121 ccatgttttt gtgccgttaa acatcatatg ttctacaaaa tgagccacgc cgcgctcatt
     3181 gtcttcttcc tgcaatgaac cggtatgaat ttgcagccat aaatttacct gatcctttgg
     3241 ttgagcatgc ggataaatca tatatcgcaa gccattgtcc agttgcccgg taattaactt
     3301 ttcatcctgc ggtaaggcgg cggcaatcag ccgcccgggg agcaacagag ttgccactaa
     3361 cgtcagtaag aaacagaggt ttctcataat tatctccatg cgaaaaccgg gcgaatttac
     3421 ccggttaagt aaaatccgaa ctattaaaat ttcatgctga catcaagcca gaaagtacgg
     3481 ccactggcgt aggtcgccac accggtgttc gttgtatcaa cagcggtttt gctatccagt
     3541 acattgagaa tatcggcact gatcgtcagg ttttgttgtt tcagaaattg tggcgtccag
     3601 gacaagcggg tgtcccaggt caggctgcta tccagttttt cgtcaacata ctgcttgtaa
     3661 tctgaatatt cgctgatgta ttgcgcattt gtcttaccaa ggataatgcg agctttacgc
     3721 gcttcttgcc aggccagcgt attcgcccac actaacccgc tcggttgatg cgtgaaatcc
     3781 atgtttaagg agatctttaa tgggttatta aaatctgcca ctggaacact atcgtaagag
     3841 accagattac cgttataaac cacctggtta tcaccggtat tgctctcctc ataaccattg
     3901 tttaacgaca agttgccctt gctcttgata tagctaaaga caatttgtgg gttaatatct
     3961 acttggcgga tatgcagggg ttcggccagt tcaaaactga ggctgaacga atgcgttttg
     4021 gttttgccat cgttgttata ttcagtaatg gtggttttag tcgcgctgtc ggtacgactg
     4081 cttttgctga tttgatcatg cgcttcacgg taaacatagt ttgcacgcgc aataacgttc
     4141 ttaccgattt tctgctgcaa tcccattgcc agttcatcgt tataaggcgt tttcaaatcc
     4201 tgataacgcg tcagagtttt attacctgat accgattccg tccagctatt gcggatatca
     4261 cgtaatccca tatcaagaat attcccgcca tagtaacggt tataaccggc ggtaatcatt
     4321 gaggtttgat cagcaaaaat atcccattcc gtcataaagc gcggggagat attgtggttt
     4381 gacagatagt tgtcatagtc ataacgcaca ccgggcatta acgacacatt ccgccagcta
     4441 atgtgctccg ccatatacag cgtgtagttg tcaattccca ggctgccttt acctttatgg
     4501 taaatggtat ggttagtttt ctttccggca gcattaatca cataggattc agactggtta
     4561 tggcgttcag tccatgcatc ggaatagatg tattccgcgc cgaagtaggg ttgatgcgaa
     4621 acatcaccca cggcgaattt ttgccagtcc aggcgtgttt tgaaggtgta attatctaca
     4681 gcctgggaaa tgtgtcctaa tccgccacag gtacaacgcc ctgtaatatc accatatgta
     4741 catgaaagtt cggtgtacca gatgtcatga tcgtgacggg tataatcact aatatgatcc
     4801 caaccaacgg tggtacgtag tttggcccat gcgagttgcg tatccatatc ccatgccaga
     4861 ccataggatt tattacccat ttcgcgatca gactgcggga aggtgctggt attataatca
     4921 cggctggagc cggtatattt taaggttaaa tcgtgggtaa agcggtcgct ggcaaaccag
     4981 gtaaatttgc tcaatgcagt atcgataacg tttttatact gtgcccgacc ggtgacaatg
     5041 ccgtcattcg aaacataatc cgcgcgggtg atatcagact ggcggcgcga taaaccggcg
     5101 gtaacgccga agttatcagc gagttcctga ttaaacgaca aggtataaaa gttcttttta
     5161 aaatctgggg agtaataagt acttcctgaa gagccttgat taaatgcgct cttgttattc
     5221 tcatcgatat gcgatgttaa ccagtccgaa cgcgtagtgc gataacccaa tttcaccttg
     5281 ctatcatcag cgttgaagcg tttgatcttt gcatcaatta ccccgccatt gaagcgacca
     5341 aattcaaccg gcacaaaact gtcataaagc gtcacattgt ccagtaagct gacatcaaga
     5401 taatacccct gtgacatccc gctaatattg gttgcactac tggcatcgga ctcattcgct
     5461 gggttcaggt tattagttgc actaataccg tcaatcaaat aggcattctg gtagggcgac
     5521 gcaccgtgaa tagagatttt ctcagggcga atatctccct ggttcaacga ggtactttgc
     5581 gttgaatcca tgcgtaccgc agggttggtt ctcagcagat cgctgatatt gccattcccg
     5641 gtcggtaaac gctcgatgct ttcactggtg taatgcgtgt tgccgtttac gggtacaggg
     5701 acaggagcat agaccgtcat ctcttcaaaa aaatgcatgt acatgttttt gtcatcgacg
     5761 gcctgcgcca catcagcgcc acataaaatg acgccaggaa taagaactcg cttcatttat
     5821 ttatatctcc gtacag
//
LOCUS       DAETQN010000200         5720 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.203, whole genome shotgun sequence.
ACCESSION   DAETQN010000200 DAETQN010000000
VERSION     DAETQN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.203"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="K0W68_004153"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="K0W68_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBI3075553.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="K0W68_004154"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="K0W68_004154"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBI3075554.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="K0W68_004155"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="K0W68_004155"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBI3075555.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="K0W68_004156"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="K0W68_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBI3075556.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1430 a         1366 c         1548 g         1376 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
     5641 ttgtgtgggc actcgaagat acggattctt aacgtcgcaa gacgaaaaat gaataccaag
     5701 tctcaagagt gaacacgtaa
//
LOCUS       DAETQN010000201         5683 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.204, whole genome shotgun sequence.
ACCESSION   DAETQN010000201 DAETQN010000000
VERSION     DAETQN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.204"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            525..1651
                     /gene="ltrA"
                     /locus_tag="K0W68_004157"
                     /pseudo
     CDS             525..1651
                     /gene="ltrA"
                     /locus_tag="K0W68_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            1734..1913
                     /locus_tag="K0W68_004158"
                     /pseudo
     CDS             1734..1913
                     /locus_tag="K0W68_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1943..2425)
                     /locus_tag="K0W68_004159"
                     /pseudo
     CDS             complement(1943..2425)
                     /locus_tag="K0W68_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2681..3661)
                     /locus_tag="K0W68_004160"
     CDS             complement(2681..3661)
                     /locus_tag="K0W68_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBI3075557.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIHLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3661..4860)
                     /locus_tag="K0W68_004161"
     CDS             complement(3661..4860)
                     /locus_tag="K0W68_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI3075558.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5182..5535)
                     /locus_tag="K0W68_004162"
                     /pseudo
     CDS             complement(5182..5535)
                     /locus_tag="K0W68_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1569 a         1296 c         1333 g         1485 t
ORIGIN      
        1 agggtttaca ggagcactga ccaaacgacc cggtgctgct tcattctaag gaatgaggtg
       61 gtgagcgact cgggaaaagg cagagatcta ctgtcacgtg gggttcggga agctgaacga
      121 aagtaaactg tccgatgaag catcgaaatc ttagatgatg tcaaaatggg gccgtacagc
      181 cgccccctga taagcgcaga agacacctgc ttactgactg cgcggcatcc ggtgtgaagg
      241 cagcaggatc ccgggccaga ccatctcagg gaacgtgaga acctgtttcc ggcacgtatc
      301 gggaaacagg ggcggaccca ttcgtagtag tgatgaaaga tctgtaatgg gtctggagcg
      361 aagggatggg gatcacattc attgcagaag acacaaccca aaaggggatg acgtaactga
      421 tgcaacgaaa atcatttgaa ataccaaaag cactggtctg ggcatcatat ctggatgtgc
      481 gtcgcaacaa gggagccccc ggatgcgacg ggcagacttt gaaaatgttc gaccagcaac
      541 gtgatggcaa tctatacaag atctggaacc ggctatgttc gggaacatgg tttccgccac
      601 cagtgctgga aaaacggatc ccgaagtcga atggcaagga acgcattctg gggatcccaa
      661 cagtatccga tcgaattgct cagggagcga taaaactttt catggaagaa aagcttgacc
      721 cgattttcca tgctgattca tatggttacc gaccaggcaa atcagctcat gacgctctga
      781 aacaatgtgc catccggtgc tggcgttata gctggatact ggaagtggac atcagtgcgt
      841 ttttcgacca tgtgagacat gatctggtgc tcaaagctct ggaacaccac gggatgccta
      901 aatgggtcat cctgtactgt cgacgctgga tggaagcgcc aatgcagagt tgtgaaaatg
      961 gagaattaat aacccgaaca cgaggcactc cgcagggcgg ggtcattagt ccgctacttg
     1021 ctaacttgtt ccatcacgat gcatttgatt tgtggatgga aagagaatat cgggggtacc
     1081 gtttgagagg tacgctgacg atattgtagt acattgttca cgaatgagtg atgcaacaag
     1141 gctgaagaac aggttatcgg agcgcttctc ggaagtcggg ctggtcctga atgcagggaa
     1201 aacgaatatc gcctacattg acacgtttaa aaggagaaac gtcgcaacga gtttcacctt
     1261 tctcggatat gacttcaaag tgcgtacgct gaagaatttc aaaggcgaac ggtaccgaaa
     1321 atgcatgccg ggtgcgtcaa atgcagcaat gcgcaaaata acagaaacaa tcaagaagtg
     1381 gcgtatacat cgctcaacag ctgagagttt gctggatttt gcgaggcgct acaatgcgat
     1441 agtgagaggc tggatcgagt actacggaaa gttctggtcc agaaatttca actatcgact
     1501 gtggagtgca atgcagtcac gtctgctcaa gtggatgcag tctaaataca gactttcgaa
     1561 ccggaaggct cagcgaaagc tgacgctggt aaggaaggag tatccgaagc tatttgttca
     1621 ctggtattta ctacgtgcat caaatgagtg atcaagagcc gtatgacgcg agagtgtcac
     1681 gtacggttcc gtgagcagcc ggggagtgaa attctctccg gctgactcga ctcgcgggtt
     1741 ccgacagcga tgacgaacat gcggcggtgt tgtactcgct gatcggcaca tgccgtctga
     1801 acaatgtgga accagaaaaa tggctgcgtt acgtcattga gcatatccag gactggctgg
     1861 caaaccgggt acgtgatctg ttgccctgga aagttgatct gagctctcag taaatatcaa
     1921 tacggttctg acgagccgct tacttttggc gtaaatctgc tgcgccttta ctgaacacaa
     1981 tgccgtattc agcaagaagt ccacgaatgt gatttatcag ttgtgtcctt tccattgaaa
     2041 ccaaccgacg catattcaaa aaagtctgga cgtcctgttg ctccagagtt tttattggta
     2101 cgggtcgtat agttccatgt tggcaggcct cagctattgc ctgtgcgtcg ttataatcat
     2161 ttttctggca ccgctgataa gctttaacat gttgtgccgg aagcaatatt acatcatggt
     2221 cgagtttcct gatttcccgg gcccagtaat gagatgcccc gccagcctcc atcgcaacag
     2281 tcattttagg ctgttgcacc agccagttaa gcagttgatt gcgagaaagt ttggtctttc
     2341 ctgcgggttt gccatgagca ttgatactga aaagataaaa gttcgtttta gccagatcaa
     2401 tgccggtgat tttctgttca gacatggtat tccgcttact tttgtggtta aagtttatcc
     2461 atcatggcgc aattgaaggc tgccgtgaaa gagggggaag actatctcat cggttactgt
     2521 taaacggcta agagaaacta tctttttcaa gcaaagaaga tacggtgaaa caggcgattt
     2581 caccgtgaaa tactgttcaa aaaaaacgct aataaaagca aaatttcttt taaaacagcc
     2641 aattaaaaac tggtgagttg ctggcgaaaa acttgaattt tcacagcgaa ttcagattat
     2701 cttttagtac aagagcaatc atacgatcta acttatcctg aacttccaac ggtaatctgc
     2761 cgaattcata tgagaacgta cgtcccttta ctcgttttcg agcaaattta tctttactat
     2821 caaatttcca taacaaagtt acaaccgctt tatcctttac tcctgaatca agcaatgaag
     2881 acatttcctt ggttataagc cgcataatac tgtttttaac ctcgtcttca gaaagattgt
     2941 catcagcagt aataagctca atactcggcg agatattctt cacaacttca tcaatagata
     3001 tatcacccag cctcagtcgt tcacttaatt cagccagttg cctgtaatcg gcgtatgtca
     3061 gctcagactg aacaggaaaa agagacacaa aatctttagg tacggacgct gcttggaggg
     3121 cacgagtcac cttagctgcc gacaatccct ctctttccgc aatttgcttc tggttcatac
     3181 cggcctcttt gagacgtact aaacgcaacc caatttcacg aatattatgt tcaagtgacg
     3241 tctgcaaatc tttcgccaga tgctgagctt cagaaacagt aagctcatca tccgttacaa
     3301 gtacacgtaa tccaaccttg catagcaaag ctgctgcccg acgacgggaa ccatccaaaa
     3361 tctcaataag atcgccagtc cgaatcccaa tacatggata gaattgttgt aaatgtatag
     3421 ttctggtgat gtcttttagt gagtccggcg tcagggctgt ctgttcgcgg ccattcacct
     3481 cttcaaccac aaaagtttga gtgtccactt tgtcatgatc aaccttgatc tcagtaaatt
     3541 ttgcctttcg gccagtattg agggtaaata catgaactgg agccgatatt tcttcagtat
     3601 tattcaggat atttgtattc agcgtacggc caatagtcgg tcggtgcttt ctgttttcca
     3661 tcagtagttg gcccttataa attcaatacg atcaaaaact gctttagcga aatcttctgc
     3721 tgccatacgt gcatttttca aagcttcacc actgccaaca taagtaacag gattcgcaga
     3781 tatcacggta tcaaacgact cgccgcttct ctcaaaacca tcaagacgtg gcattgaaac
     3841 atcaagcatg tcaccaccga aaatctcttt tgtcaggcta tggcagtatt tatgatctga
     3901 cttattcgcc agtttcgaca tgaagccaat atttgcctgc aaacggcatg aacatccatc
     3961 ctgttcaata atctgtacaa gttcaggtaa acgagccaaa tatttaagcg tggaatggaa
     4021 atcaacttgt gctggcggca ccggtgtaaa catgatatct gcggcagcaa tggcattttt
     4081 caaaaatgcg tccagatgcg ggcctgtatc aataaggatg aagtcgaaat catgcttcag
     4141 cttatcaata atattctcac gcaatatcgc gtgtttgttt tgtcctaata gatgctcctc
     4201 gcacagagta tcccagtttg atgcaataaa ggcgtcgtca atagaagccg gcataacata
     4261 aacgccaggg ataactgacg gaacgataaa atcttccagt agttcctcac gactcacgtt
     4321 ctgcaacata gcctgcggtg cggttgtatc aaccagcccc accgcatgca agtagttcag
     4381 aaacatagtg gctgatgact ggggatctaa atcaaggagc agaatacgta aatcttctga
     4441 cagcaggtga ggatgagcac gcagggcgtg agctacggaa acactactca cggttttaga
     4501 cacaccgcct tttagactac cgatgaatat ggaatatgcc tcactataac gatcgcgata
     4561 tttaggaatg ccacgatgtg catagatatc gataatgttc tggatggtta atgcatattt
     4621 tgtcgcggtt cctgctgggc gtttttcaaa ctgatagccc tgagcctcca tctcaccgac
     4681 tgcaccatcc acgcttgcgc gcgttaattt tggaagctta gctacagcag actttgaata
     4741 gacttgataa aaaacatcag cctgaaactc ctgcttttgc tcctgaatct gttttgttag
     4801 ggcgagcaac attttatctg cacgctgcgc aaccttactt aactgctcaa aactagtcat
     4861 tccccctccg tgctatgtag gattttaact tattacaaaa aaaactgcat tagcagggta
     4921 atgcatgcaa gtacatattg tacatgctta tattaaaaat gcgcgcattg ctgatttatt
     4981 aatcatcgga atgaatatta ctgtaagata gtatgccttt ccgggagaaa gacgctgatt
     5041 tcccccgaat aatccagttc accaccacac tccggacaac agctttcttc aggctccaga
     5101 tggtgtattt cgcgggaaac atgttccggt aacggacggc gatgacgtga ctgtcgcaac
     5161 aggcgggggt agtgcttcca atcagtagaa catgtgcttt tcaatgaagg agccgatgac
     5221 tttttcgtgg atttccacgg aacgcgagaa gcagattgtt ttacgtgcca gccgcttgat
     5281 gcgggtgcgg agggtccggt tgttacgttc aatgcgttgc gtgaagattt tgccggtcag
     5341 gtgtttctcc ttcggcactt ctctggcgta gctgccccag tcatcgctgg tcaccatgcc
     5401 accatgccga tacagaacgg agtcaacagg gctaacagtt cccgacaggt ctcatcattc
     5461 cgtggcccga aagtcgaaag tgtaagccaa cacaccgcca gttttagtgt tgtacgcata
     5521 ccagagccag tgttgtgtca acgacggatg aaaagtgatc cacttatatc tccaccaacg
     5581 gcccaatatt gatccaccgt tttactcagg attagcttct gctataaccc cggcctttcg
     5641 tttctgtctg agtcgatagc tttctccttt gatttgaacg aaa
//
LOCUS       DAETQN010000202         5620 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.205, whole genome shotgun sequence.
ACCESSION   DAETQN010000202 DAETQN010000000
VERSION     DAETQN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.205"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..513)
                     /locus_tag="K0W68_004163"
     CDS             complement(<1..513)
                     /locus_tag="K0W68_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBI3075559.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="K0W68_004164"
     CDS             complement(759..1211)
                     /locus_tag="K0W68_004164"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3075560.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="K0W68_004165"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="K0W68_004165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBI3075561.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="K0W68_004166"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="K0W68_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBI3075562.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="K0W68_004167"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="K0W68_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="K0W68_004168"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="K0W68_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HBI3075563.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5620)
                     /locus_tag="K0W68_004169"
     CDS             complement(5494..>5620)
                     /locus_tag="K0W68_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3075564.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1468 c         1460 g         1353 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgatgtgc
//
LOCUS       DAETQN010000203         5614 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.206, whole genome shotgun sequence.
ACCESSION   DAETQN010000203 DAETQN010000000
VERSION     DAETQN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.206"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            12..428
                     /gene="soxR"
                     /locus_tag="K0W68_004170"
     CDS             12..428
                     /gene="soxR"
                     /locus_tag="K0W68_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBI3075565.1"
                     /translation="MAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYVAII
                     KIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDELDG
                     CIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="K0W68_004171"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="K0W68_004171"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBI3075566.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="K0W68_004172"
     CDS             2475..4124
                     /locus_tag="K0W68_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBI3075567.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5569)
                     /locus_tag="K0W68_004173"
                     /pseudo
     CDS             complement(4278..5569)
                     /locus_tag="K0W68_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1256 a         1270 c         1456 g         1632 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc tctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt agagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg acattattta taaaatatac caccttaatg gatacttcct
     5221 tttctttact aaggtaaact cttctcgtaa ttctatcgtc acatttatta tttgcatata
     5281 aagctatatc tataaaatgt ttatgtattt tcctgtccgt ttccttcgtg atttttcctg
     5341 atggtaaact atcataaatt cttttcattt tctgaatcaa acctatcact ttttcatgat
     5401 catccatctt gaaaagagag agggctttat tgtcaattaa cgtgttttta tgatgtttgc
     5461 tgtaattata tgattttgtt ttctttgtat gtgatgaact tgtgaaagag tggttatcgt
     5521 taatctggca aagagagaaa aacatattat ttaaaccatt atagcgcata aaatatcctt
     5581 ataattaaca agaaagaaaa ggcatattct ctgc
//
LOCUS       DAETQN010000204         5527 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.207, whole genome shotgun sequence.
ACCESSION   DAETQN010000204 DAETQN010000000
VERSION     DAETQN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.207"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="K0W68_004174"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="K0W68_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBI3075568.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="K0W68_004175"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="K0W68_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HBI3075569.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="K0W68_004176"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="K0W68_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBI3075570.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="K0W68_004177"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="K0W68_004177"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBI3075571.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="K0W68_004178"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="K0W68_004178"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBI3075572.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="K0W68_004179"
     CDS             4447..4812
                     /locus_tag="K0W68_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBI3075573.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="K0W68_004180"
     CDS             4962..5480
                     /locus_tag="K0W68_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBI3075574.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1520 a         1391 c         1240 g         1376 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatatttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgca
//
LOCUS       DAETQN010000205         5296 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.208, whole genome shotgun sequence.
ACCESSION   DAETQN010000205 DAETQN010000000
VERSION     DAETQN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.208"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            356..981
                     /gene="gstB"
                     /locus_tag="K0W68_004181"
                     /pseudo
     CDS             356..981
                     /gene="gstB"
                     /locus_tag="K0W68_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(978..2093)
                     /gene="yliI"
                     /locus_tag="K0W68_004182"
     CDS             complement(978..2093)
                     /gene="yliI"
                     /locus_tag="K0W68_004182"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HBI3075575.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="K0W68_004183"
     CDS             complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="K0W68_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBI3075576.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2800..4125
                     /gene="rimO"
                     /locus_tag="K0W68_004184"
     CDS             2800..4125
                     /gene="rimO"
                     /locus_tag="K0W68_004184"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBI3075577.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4172..5296)
                     /locus_tag="K0W68_004185"
                     /pseudo
     CDS             complement(4172..5296)
                     /locus_tag="K0W68_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         1384 a         1331 c         1261 g         1320 t
ORIGIN      
        1 cgctcggcgc ttcaacggtt tgttccgccg cgaatgccgt tggggcaaaa aggaataaaa
       61 atgcagaacc cgctgcaaga ccacgaagga gagaggagta ttgcgtcata aatgccaccc
      121 gagtatccat tcaaaaaaaa tacgttatat cgccgtatga taagttcggt gattaataac
      181 gcacacacaa acttaaagaa accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc
      241 tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa gttaacttta
      301 cttcaccatc ggagtcctgt tcagcctgtg gctgcctgtc ggaaaagggg tgagtatgat
      361 tacgctgtgg ggtcggaata attcaactaa cgtaaaaaag tattgctgac gctcgaagaa
      421 ctggaactac cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct
      481 gattttctgg cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat
      541 ctcattcttt gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa
      601 cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca
      661 aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg
      721 gaagagcgcg atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc
      781 ctgctcgatg cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc
      841 gatatcgcta tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg
      901 cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg
      961 gtgatgattc ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg
     1021 tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct
     1081 ctgtccgtta aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg
     1141 acatctttat ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg
     1201 ctgttataga aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc
     1261 tcggtcccgg cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt
     1321 ccccaggttg ccagcggcca gccgtagtgt ttgccttttt gcgggatatt aatttcatca
     1381 ccaccgcgcg ggccatgttc attcagccac agtgcattac tccacggatt catcgccatt
     1441 ccttgcggat tacgaatgcc ataagaccag atctcggcgc gcgcaccgga ttcctttata
     1501 aaaggattat catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat
     1561 ttatccagat cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa
     1621 cctttaccgt cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc
     1681 tggcggaaga cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa
     1741 cccacagcag ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg
     1801 cgagactgag caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc
     1861 caaacgtccg gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc
     1921 aactcgccgc cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc
     1981 cagggatggt cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag
     2041 agagcggaag aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct
     2101 ccggattgac tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa
     2161 aaacttaaac atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca
     2221 tggcgtgctt ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct
     2281 tatcggcgtt gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg
     2341 cggctgtcgc taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat
     2401 gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc
     2461 gtagatgatt gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg
     2521 cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa
     2581 cgaacatact tcgatcctcc tctttccagc cttgttgcga ttaaatagta tggctatttt
     2641 ttgagcaaaa tgctgtccag gatcaattct tcacgccaaa ttaacaaaat ttacatttcc
     2701 ttcgcctccg ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc
     2761 ctgccctgac ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag
     2821 ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt
     2881 ctcaccgaac tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg
     2941 gtgatcgtca acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt
     3001 ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa
     3061 gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag
     3121 caggttctgg agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc
     3181 ctggtgccag aacaaggtgt gaaactgaca ccgcgtcatt atgcctatct gaaaatttcg
     3241 gaaggctgta atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg
     3301 agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa
     3361 gagattctgg tgatctcgca ggatacttcc gcctatggcg ttgatgttaa acatcgtact
     3421 ggcttccaca acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg
     3481 aaactgggga tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc
     3541 atcccactga tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc
     3601 agcccgcgca ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc
     3661 atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc
     3721 ttccctagcg agacggaaga agatttccag atgctactcg acttcctgaa agaagcgcgt
     3781 ctggatcgcg ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg
     3841 cctgaccagg ttccggaaga agtgaaagaa gaacgctgga accgtttcat gcagctgcag
     3901 cagcagattt ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc
     3961 gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac
     4021 ggcgcggttt atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa
     4081 gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg
     4141 caaaatcatt tgtccgccct gcgaataatg attactgcca gctaatctga gccctgccgt
     4201 ttttcttact cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac
     4261 attcatttga atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat
     4321 gaacgatttc ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg
     4381 caaattcgtc accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa
     4441 ggattttcac cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa
     4501 tgtttttgaa tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt
     4561 ttaaatcttc gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg
     4621 agttatacat actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca
     4681 gtacgcgttc aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca
     4741 ctctgcgtac cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga
     4801 aaataatcaa tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc
     4861 cggtcggcaa ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag
     4921 cattatattc atcaccggtt taccgttctt aaatagagag aacatcaggt tctcgctgtc
     4981 catcgccagc gattttgaca gtaaagaaaa acgctccaga ccaagcaaaa atatcagact
     5041 acgattgtga gttaccggat agaggtaata gacgaaggtt ttaccgtcaa aagagataaa
     5101 ccctgtattt ttgacatgtc gatacttcac aacctgcata atatctgcac aataattttt
     5161 ctcgaactcc tttacctgaa tacattcgtc actggaaatc agataaggac cgacatcgta
     5221 caaataagag ggggaaaccg agtcggcgta agctgtgcgc aaaccaacca acagctgttc
     5281 ctccgtggga tttagc
//
LOCUS       DAETQN010000206         5281 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.209, whole genome shotgun sequence.
ACCESSION   DAETQN010000206 DAETQN010000000
VERSION     DAETQN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.209"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..471)
                     /locus_tag="K0W68_004186"
     CDS             complement(<1..471)
                     /locus_tag="K0W68_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI3075578.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
     gene            complement(471..1391)
                     /locus_tag="K0W68_004187"
     CDS             complement(471..1391)
                     /locus_tag="K0W68_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI3075579.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1529..2428
                     /gene="pgrR"
                     /locus_tag="K0W68_004188"
     CDS             1529..2428
                     /gene="pgrR"
                     /locus_tag="K0W68_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBI3075580.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2765..4378
                     /gene="mppA"
                     /locus_tag="K0W68_004189"
     CDS             2765..4378
                     /gene="mppA"
                     /locus_tag="K0W68_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBI3075581.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTARFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4429..>5281)
                     /gene="ynaI"
                     /locus_tag="K0W68_004190"
     CDS             complement(4429..>5281)
                     /gene="ynaI"
                     /locus_tag="K0W68_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBI3075582.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1430 a         1203 c         1225 g         1423 t
ORIGIN      
        1 gccaatcgga tcgtaattgc tgaaaccttc ccgaaaaaga cgcccgatat taacacccgt
       61 aatactcact actgctttaa ttcgtttttc tgtcagcgcg gcattaatgg catagccacc
      121 acccccacac actcccagca cgccaatacg ttcagcatta acatatggca gagttactgc
      181 ataatcgata acacggctaa tatcctctac gcgctgtgtc ggatcttcta tccagcgagg
      241 ctccccacca gattctccct ggaaactggc gtcataagcg agaacgagat agcctttttc
      301 agccaatgcc ttgccataga tattgccaga cgtctgctcc ttacaactgc caaaaggatg
      361 gacgctgata atcgccggat actgttttga ctcatcaaaa tttggtggaa actgaatatt
      421 ggcagcaata tgccaggcca tatcactatt gcggatttga atatgtttca ttacagagtt
      481 tcctcaaaga acggagctaa tactgaaatt gcttcagcca cataggcttt gccgtcataa
      541 agatccatat ggtttgcgcc ttcaacaatg tgatagcgtt tatcttggct tgatgctcga
      601 tcgtacaggt cgtcactcat ccatttgctc cctgcctggc tgcccgccac aatctgcatc
      661 ggctgagtca ggtacacttc cgccatatgg taagcatcaa aggtaataat ctggttaagg
      721 ctgcgcaaag tagcgtaacc cggtgctgtt ggatactgcg cgcgaggggt gtggtaatac
      781 tcccaggcct gacgcagttc ttcgttcggc gcatcggact ccttcattgg tgccagtggc
      841 ataacggcat attctccgct gctaatatcg ctggttcggg cgtttgaacc cgcttcaacg
      901 tatggcaggg catcaataga tttcacattg ttttcccaac cattacggaa catcgaacca
      961 atattgaccg cactaacggt accgatggcc ttgatgcggc gatcctgaat tgcagcattg
     1021 gctgtatatc ctgcaccggc acaaattccc atcgcaccaa ttcgggtatt gtcgacataa
     1081 gaaagcgttg tcaggtaatc aatcacggca ctgatgtctt cagtacgaat gtatgggttt
     1141 tctaactgac gcggctcgcc gccactttca ccttgataag atgcgtcata agcaatagtg
     1201 acaaatccct tttccgccag ttttttggca taggttccgg ccgtttgttc tttaacgccc
     1261 ccacctgggt gagataccac aattgcctga tactgacggg tttcatcaaa ttttggaggg
     1321 aaatagatca ctgcagacaa agagatggtt ggattattgc tgttagtgaa gctgacttta
     1381 ttattcatca ttccgttcct catggagttg tcggttcgtt ttaacggttg gtgatatcac
     1441 tatagatatt gatcattaag ttgattagac ccaaaattat gattagacct atcatttaaa
     1501 tgattaatag attgcatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc
     1561 gtttgtcgtc gttgcagagg agcgtagctt cactcgtgca gcagcccgcc tgagcatggc
     1621 gcagtcagct ttaagccaga tagtgcgtcg tatagaagaa cgattgggat tgcggcttct
     1681 gacgcgaacc acgcgcagcg ttgttccaac tgaagcgggc gagcatcttt tgtctgttct
     1741 tggccctatg ttgcatgaca tagattcagc cctggcatcc ctgagcgagc tgcagaaccg
     1801 cccatccggg acaatacgta ttactactgt agaacatgca gccaaaacga tattgttacc
     1861 agcaatgcgc acattcctga aatcgcatcc tgaaattgat attcagctca ccattgatta
     1921 tggtttgacc gatgtcgttt ctgaacgttt tgatgcaggc gtccgtctgg gtggggagat
     1981 ggataaagat atgatcgcca ttcgaatcgg gccagatata ccaatggcta ttgttggctc
     2041 accggattat ttttctcgcc gaagtgttcc aacgtcagtg tcacaattaa tagatcatca
     2101 ggcaattaat ttgtatcttc ccacatcggg tacagcaaat cgctggagat taatacgcgg
     2161 tggacgtgaa gttcgtgttc gcatggaagg tcagctttta ctgaatacga tagacctgat
     2221 cattgatgct gcaattgatg ggcatggatt ggcgtatcta ccttatgatc aggttgagcg
     2281 ggctattaaa gaaaaaaaac tgatacgtgt tttggataaa ttcacaccag atttacccgg
     2341 ttatcacctg tactatccac accgtcgaca tgctggctcg gcattctcat tatttataga
     2401 taggctgaag tataaaggtg ctgtttagca ctacttgctg atacattaat ttaatttttc
     2461 tcttaacgta ttctcagttc ctttcaacgt tttggttatt ttttattctt cgtacaatgg
     2521 cgacagatgc tgattatgat accgaaaact ggtttgaacg tgcgaagcct gaacgtagtg
     2581 ttcggtgttc tatgtgcttt accgcatttt gtcgtctatt atttacacta aatctgattt
     2641 aatatattga tacataaaac attttatgct ccctgtgtca cttttttgat ggaagttgtt
     2701 tgcatttctt taaggcgaaa caaataatta cgcatcaatt ttaatgtcgg ttagagggaa
     2761 acttatgaag cactctgttt cagtcacgtg ttgtgcgctg ttggtcagca gcatttctct
     2821 ttcgtatgct gcagaagttc cgagcggcac agtactggca gagaagcagg agctggtgcg
     2881 ccacattaaa gatgagcctg cgtcgctgga tcccgctaaa gccgtgggcc tgccagagat
     2941 tcaggtcatt cgcgatctgt ttgaaggact ggtgaatcag aacgagaaag gggagattgt
     3001 ccccggcgtt gcgactcagt ggaaaagtaa tgacaaccgt atctggactt ttaccctgcg
     3061 cgataacgca aaatgggcgg atggcacacc ggtaacggcg caagattttg tctacagctg
     3121 gcagcgtctg gtggacccga aaacgctatc gccatttgca tggtttgccg cgctggcggg
     3181 aatcaacaac gcacaggcga ttattgatgg taaagctacg ccagaccagc ttggcgtcac
     3241 cgcagttgat gcccatactt tgaaaattca gcttgataaa ccgttgccgt ggtttgtgaa
     3301 tttaaccgct aactttgcct tcttcccggt gcaaaaagcc aacgtagaaa gcggtaaaga
     3361 gtggacgaaa cccggaaatc tgatcggcaa tggcgcttat gttcttaaag atcgcgtagt
     3421 caatgaaaaa ctggtcgtgg taccgaatac ccattattgg gataacgcca aaacggtact
     3481 gcaaaaagtg accttcctgc caattaatca ggaatccgca gccactaagc gttacctcgc
     3541 gggggatatt gatatcaccg aatccttccc gaaaaatatg tatcagaagc tgttgaagga
     3601 tattccgggg caggtttata cgccgccgca gctcgggacc tattattatg cgtttaacac
     3661 gcaaaaaggg ccgacggcag atcagcgcgt tcgtctggca ttaagtatga cgatagatcg
     3721 ccgcctgatg accgaaaaag tattagggac gggcgaaaag ccagcgtggc attttacacc
     3781 agatgttacc gccagattta cgccggaacc ttcgccgttt gaacaaatga gtcaggaaga
     3841 actgaatgcg caggcaaaaa ctttgttgag cgcagctggt tatggtccgc aaaaaccgct
     3901 gaagctgacg cttttgtata acacttcaga aaaccatcaa aaaattgcga ttgctgtagc
     3961 atcgatgtgg aaaaagaacc ttggcgtaga tgttaaattg caaaatcagg aatggaaaac
     4021 ctatatcgat agccgtaaca ccggcaattt tgatgttatc cgcgcctcat gggtggggga
     4081 ttataatgaa ccctccactt tcctgacatt attaacgtca acgcattcag gaaatatttc
     4141 acgctttaac aatccggcat acgacaaagt tctggcccag gcatcgacgg aaaataccgt
     4201 taaagcgcgt aatgccgatt acaacgcggc agaaaaaatc ctcatggagc aagcaccgat
     4261 tgcaccaatt tatcaatata ccaatggacg attaatcaag ccgtggctga aaggttatcc
     4321 cattaataac ccggaagatg tggcgtacag ccggactatg tatattgtga agcattgatg
     4381 tgatgggaac tggcgttacc cttgtggata acgccagtga tgtctgattt agcgcccctg
     4441 ttccggcggt gtaatgttat ccatatacag cgtctggctg gggaaggcaa agtccgcgcc
     4501 gtgtgactgc acaatatcga taatcttcaa ataaacgtct tgctgtgcag caagccattc
     4561 agcccatacc gtggttttgg taaagcaata aaccataata ttcaatgacg agtcggcaaa
     4621 ctggttgaaa taaaccagta aggtttgtcg ctggtcgatg gccgggtgat ttttcagcat
     4681 ctcacgtacg gcttcgacaa taacgcccac ttttgccgca tcctcataac gtaaaccaat
     4741 ggtcgtggta atgcggcggt tggtcattcg tcctgggttt tctacgctga tcgacgaaaa
     4801 cagcgagttc ggtacgtaca atggacgatt atcaaaggtc gtaattttgg taattcgcca
     4861 gccaatttcc gctactgtac cttcgatatt tctgtccggt gaacggatcc agtcgccaat
     4921 actgaaagga cggtcgaaat agagcatgat cccggaaaag aaattactca gaatatcttt
     4981 accggccata ccgacagcca gaccaccaat accaccaaag gtcagcaagc cagaaaggct
     5041 catgccgaaa tgttcgccat aaagcagaac aagcaccaca ataatggtga ttttgataat
     5101 gcgcgacata atccgcgcac tggtgatatc gcgacctttt ttaatctgct gtttttcaaa
     5161 ctgattaatc agcaaaaata gcttaatcgt cagaataacc gcaatcaggg acgtacagat
     5221 aaaatcgata acgtctgggg tgataaattt gagtttatag ttttctataa cataattaat
     5281 a
//
LOCUS       DAETQN010000207         5244 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.210, whole genome shotgun sequence.
ACCESSION   DAETQN010000207 DAETQN010000000
VERSION     DAETQN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.210"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..1133)
                     /locus_tag="K0W68_004191"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="K0W68_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /locus_tag="K0W68_004192"
     CDS             complement(1133..1315)
                     /locus_tag="K0W68_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075583.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="K0W68_004193"
     CDS             complement(1327..3060)
                     /locus_tag="K0W68_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBI3075584.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="K0W68_004194"
     CDS             complement(3057..3551)
                     /locus_tag="K0W68_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBI3075585.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="K0W68_004195"
     CDS             complement(3673..4023)
                     /locus_tag="K0W68_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBI3075586.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="K0W68_004196"
     CDS             complement(4066..4530)
                     /locus_tag="K0W68_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBI3075587.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="K0W68_004197"
     CDS             complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="K0W68_004197"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HBI3075588.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(5024..5239)
                     /locus_tag="K0W68_004198"
     CDS             complement(5024..5239)
                     /locus_tag="K0W68_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBI3075589.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT         1148 a         1534 c         1299 g         1263 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgcttaccgc cagcatatgt ccagcgacgt
     4621 agctggtcac atgcgccttt gatatcgccc tggtttattt tgcgaagaag cgtcgatgtt
     4681 ctgaaattgc cagcgcccac gttgtaaacg aacgagtaaa gagcgccgcg cgttgtttcc
     4741 ggtatatcga ctttgatgta cgggttaatt tgtctggcga cagtggcaag gtctttattc
     4801 aggagggctt tgcattctgc ttcggtatac gttttaccga gcatgatgtc ttttccggtg
     4861 tgtccgtgac atacagtcca tacaccaatg atatctttgt atggtatgta gctgacacct
     4921 tccagaccat cgttaccacc tgggccagtg attaacacag atgctatagc aacagcccca
     4981 ccaccaatag cagctgcaac agccttgcgt aatgatggcg acattattca cctctcgcag
     5041 ccttacgctt gtcttctctt attttgaaat acagatttgt cagataagtc agaagcccca
     5101 gaagcagact tcccagcaca ccaatcgcag cccactgtga tggactgacc tgatccaacc
     5161 actgcaaaaa ccagtagccg gcactgccgg cggaggtgcc gtaggcaatg cctgttgata
     5221 ttttttccat ctgatacata tccc
//
LOCUS       DAETQN010000208         5219 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.211, whole genome shotgun sequence.
ACCESSION   DAETQN010000208 DAETQN010000000
VERSION     DAETQN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.211"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..227
                     /locus_tag="K0W68_004199"
     CDS             <1..227
                     /locus_tag="K0W68_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075590.1"
                     /translation="KNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPA
                     IIHSALNPTHYYQPVNDETTVMEIIKAYSK"
     gene            complement(291..575)
                     /locus_tag="K0W68_004200"
     CDS             complement(291..575)
                     /locus_tag="K0W68_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075591.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIA
                     FIWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
     gene            complement(593..790)
                     /locus_tag="K0W68_004201"
     CDS             complement(593..790)
                     /locus_tag="K0W68_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075592.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSV
                     SGLDVELIDVSCESEWEEHSH"
     gene            1002..1298
                     /locus_tag="K0W68_004202"
     CDS             1002..1298
                     /locus_tag="K0W68_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075593.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLT
                     QADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
     gene            complement(1843..2076)
                     /locus_tag="K0W68_004203"
     CDS             complement(1843..2076)
                     /locus_tag="K0W68_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBI3075594.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(2083..2613)
                     /locus_tag="K0W68_004204"
     CDS             complement(2083..2613)
                     /locus_tag="K0W68_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HBI3075595.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETILNDEFRKLEKSVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            complement(2591..3349)
                     /locus_tag="K0W68_004205"
                     /pseudo
     CDS             complement(2591..3349)
                     /locus_tag="K0W68_004205"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3291..3614)
                     /locus_tag="K0W68_004206"
     CDS             complement(3291..3614)
                     /locus_tag="K0W68_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBI3075596.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            3679..3870
                     /locus_tag="K0W68_004207"
     CDS             3679..3870
                     /locus_tag="K0W68_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBI3075597.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
BASE COUNT         1373 a         1427 c         1116 g         1303 t
ORIGIN      
        1 aaaaaaacaa aggcgtcata atagaagtaa acacagatag tttatgtaag aaaaaactaa
       61 acgaaataac tagtaaaaat atagggaaac ccctgatttc atattttgat ggaaaagtaa
      121 tatcccaacc tgcaatcatt cattctgcac taaatccaac ccattattat caacctgtga
      181 acgatgaaac aacagtgatg gaaatcataa aagcatattc aaaataatat taatagatat
      241 aatcctgctt aaggtatagc ttaggcagga ttatatctat tcagctctta ttataaggta
      301 gtgtatacat gaataatctg ccctgtccca ctgacacttt taaatttaat tttatcaaca
      361 ccagcgccct tgcaagagat ctgataagat gctggaccaa taacgttaac tgttcttggc
      421 ccattagggg cataccagat aaaagcaata aatggaccgg gtacattaga caatgtatca
      481 aaatagacag tgtacgcgat atcaccagta tgattttcaa gttcaaccca aggaccaaaa
      541 aagaaaggag gagcccaaac attttgagaa atcatataat ctcctgatta gttcaatgcg
      601 aatgttcttc ccattcactc tcgcaagaaa catctataag ctcaacatct agcccactaa
      661 cagaatcaga gaaatactta taaacatcat cccttgttcc cataaggtct gccgaacatg
      721 atagttcaca accattctta ttcctaacta caaataagaa acggtgaagc aatacttttt
      781 cttctttcat tttatccctc cctttattta cacagaatat actgtatata taaacactag
      841 ttttatatac agtataaatt acgccgcatt atttttcctg tcaatgatat gaattttcat
      901 ttttgtgatc tgaatcaccc ttataaaaat caggaaggga agattcgcag cagaaaaaca
      961 gcaccggata acatcaggag aaaaaacaga gaggagataa cgtgagcaaa acaaaatctg
     1021 gtcgccaccg actgagcaaa acagacaaac gcctgctggc tgcacttatc gctgccggat
     1081 acgaagagcg gacagcccgt gacctcatcc agaaacacgt ttacacactg acacaggccg
     1141 acctgcgcca tctggtcagt gaaatcagta acggtgtggg acagtcacag gcctacgatg
     1201 cgatttacca ggcaagacgc attcgtctcg cccgtaaatt cctgaacgga aaaaaaccgg
     1261 aagaagggct gaacgcctgg aagggtgatt taccataatc acttattgta cgcaccgctg
     1321 aaacgcgttc ggcgcgatca cggcagcaga caggtaaaaa tggcaacaaa ccacccgaaa
     1381 aactgccgcg atcgtacccg gtaaatttta accacatgca tagctatgca gctatgtgaa
     1441 tcatgcagga attgccagcc agagacagct gaaacggatt tttttcatgc tttcggaagc
     1501 gaagaagacg gggaccggag cgggaaaaca gaagttcagg ggaatgagcg cgatctggca
     1561 atagaggcaa aacacagcaa caaaagacac accagaatcg cgcccgtatg cgttttaacg
     1621 cgttcctgtg ttttcagcaa ggtctgactg aagcgcgttc ccggtgcccc cgaaccaacc
     1681 attatcccaa aaaatgcacc ggaaacgcgc tgacaacatt ttagcctgtc tctgattacc
     1741 atcccagcga agggccatcc agtgtccgtt cctgtatttc cagctggcgc tcacgctcca
     1801 gggataacgc atgttcgcgt tcctgtatca gccgttcgtc ccttatctcc tgttctcgct
     1861 gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc tgcacctgct gactcaactg
     1921 catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca ttttctgcaa
     1981 ttccacgaag gcgctctccc attcgttcag ccgctgcata tagtcctgtt gcagttgctc
     2041 taatgcgttc agcaaatgtc tttccagctc cgtcatgctc tgttactcct gtaccagcct
     2101 tacggcattc tgtttcccgt tttccatcgt ccagttcgtt tcggctttca tcccctcggg
     2161 caacaccaga aaacgtccgt tgctcccctc gtgataacgc acgccccatg tcttcgcgtt
     2221 cagcttctcc agattctcgt tctgcttcct gatttcttcc aggttcgcct gtatcatccc
     2281 gccaagatac cagagcgtcc cgccactcac ggtaaacagg aaaatgacca tccccagtaa
     2341 catcatgccc gtattccctg ccagctttaa cacgtccttc ctgtgctgca tcatcgcctc
     2401 tttcacccct tcccggtgtt ttttcagtga ttcctctgtc gaagctgtga acagggctat
     2461 agcgtctttg attttcgtct cgtttgatgt cacagccttg cttacagatt tttcgagctt
     2521 cctgaactcg tcgttcagta ttgtctctgt agattcggct ctctctttca gctttttctc
     2581 gaactccgcg cccgtctgta aaagattgct cataaaatgc tcctttcagc ctgatattct
     2641 tcccaccgtt cgggtctgca atgctgatac tgcttcgcgt caccctgacc acttcaagcc
     2701 ctgcctctgt gagcgcctga atcacatcct gacggccttt tatctccccg acatgataaa
     2761 gagcgtctat cccgcgtgtg acgctctctg caagcgcctg tttcgtttcc ggcaggttat
     2821 cagagagagt cagtatccgg cggttctccg gggcgttggg gtcatgcagc ccgtaatggt
     2881 gattcaccag cgtctgccag gcatcaattc tcggcctgtc tgcccggtcg tagtacggct
     2941 ggaggcgttt tccggtctgt agctccatgt tcggaatgac aaaattcagc tcaagccgtc
     3001 ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcgaga ccgggcatca
     3061 gtacacgctc aaagctcgcc atcacctttt cacgtcctcc cggtggcagc tccttctccg
     3121 cgaacgacag cacaccggac gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc
     3181 gtacttcttc cggattaccc tgaagcaccg ttgccccttc ccggttacgc tcccttccca
     3241 gcaggtaatc aaccggacca ctgccaccac cttttccccg ggcatgaaac ttaactatca
     3301 tcccgcgtcc cctgttccct gacggccaga cgcagccggc gcagctcatc cccgatggcc
     3361 atcagtgcgg ccaccacctg aacccggtca ccggaagacc actgcccgct gttcaccttc
     3421 ctcgctgtct gattcaggtt attcccgatg gcggccagct gacgcagtaa cggcggtgcc
     3481 agtgtcggca gcctcccgga acgggcaacc ggctcaccca ggcagacccg ccgcatccat
     3541 accgccagct gtttaccctc acagcgttca agtaaccggg catgttcatc atcagtaacc
     3601 cgtattgtga gcatcctctc gcgtttcatc ggtatcatta tccccatgaa cagaaatccc
     3661 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     3721 ataagaagcc agacattaac gctgctggag aagctcaacg aactggacgc ggatgaacag
     3781 gcagatatct gtgaatcgct tcacgaccac gccgatgagc tttaccgcag ttgcctcgct
     3841 cgcttcgggg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     3901 tgtctgtgag cggatgccgg gagctgacaa gcccgtcagg gcgcgtcagc aggttttagc
     3961 gggtgtcggg gcgcagccct gacccagtca cgtagcgata gcggagtgta tactggctta
     4021 accatgcggc atcagtgcgg attgtatgaa aagtacgcca tgccgggtgt gaaatgccgc
     4081 acagatgcgt aaggagaaaa tgcacgtcca ggcgcttttc cgcttcctcg ctcactgact
     4141 cgctacgctc ggtcgttcga ctgcggcgag cggtatctac tcactcaaaa acgaaatgct
     4201 gttatccaca gaatcagggg ataacaccgg aaaaaacatg tgagcaaaag accaggaaca
     4261 ggaagaaggc cgcgccgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4321 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg acttaaagat accaggcgtt
     4381 tccccccgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4621 aacgccactg gcagcagcca ttggtaacta aaaagtggat ttagatacgc agaactcttg
     4681 aagttgaagc cttatagcgg ctacactgaa aggacatctt ttggtatctg tgctccactt
     4741 aagccagcta cccggttagg taatctgcca ggattagcta accttcgaaa aaccacctgc
     4801 cagggtggtt ttttcgttta caggcagcag attacgcgca gaaaaaaagg atctcaagaa
     4861 gatcctttga tcttttttct ctaaactgca atcccatcag ttcaggagag aaagatagta
     4921 taattgccct ctcttatatg gaaccaccat tcaatcacat catgggaata atataagtga
     4981 aaacgttcgc attgtttatt tttacgtgta tatgtattta tagattggaa ttttaacaaa
     5041 ataaaatgga gttaatatgt tcaaatcatc agtaaagtca ataacatcga gtactatact
     5101 gaccttactt ataggatgtt caagtactga aacaaacaaa aacattgttt acaaagttgg
     5161 tggtgacagc atcattctga ataaatattg catacttaaa atgtaccctt cccaatcag
//
LOCUS       DAETQN010000209         5164 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.212, whole genome shotgun sequence.
ACCESSION   DAETQN010000209 DAETQN010000000
VERSION     DAETQN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.212"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            82..197
                     /gene="rrf"
                     /locus_tag="K0W68_004208"
     rRNA            82..197
                     /gene="rrf"
                     /locus_tag="K0W68_004208"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(315..758)
                     /locus_tag="K0W68_004209"
     CDS             complement(315..758)
                     /locus_tag="K0W68_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBI3075598.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            915..1844
                     /gene="metA"
                     /locus_tag="K0W68_004210"
     CDS             915..1844
                     /gene="metA"
                     /locus_tag="K0W68_004210"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBI3075599.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            2113..3714
                     /gene="aceB"
                     /locus_tag="K0W68_004211"
                     /pseudo
     CDS             2113..3714
                     /gene="aceB"
                     /locus_tag="K0W68_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3744..5035
                     /gene="aceA"
                     /locus_tag="K0W68_004212"
                     /pseudo
     CDS             3744..5035
                     /gene="aceA"
                     /locus_tag="K0W68_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(5060..>5164)
                     /locus_tag="K0W68_004213"
     CDS             complement(5060..>5164)
                     /locus_tag="K0W68_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3075600.1"
                     /translation="QHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         1288 a         1352 c         1316 g         1208 t
ORIGIN      
        1 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
       61 ggtctgataa aacagaattt gcctggcggc cgtagcgcgg tggtcccacc tgaccccatg
      121 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
      181 gtagggaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
      241 attggcgcag aaaaaaatgc cgggtgcgac gctggcgcgt cttctccagc ctgggcaggc
      301 acggcagggg cagcttattc ctccacatac gccagattca gcaacggata cggtttcccc
      361 aaatcgtcca cctcagagcg tcccgtaacc ttaaaaccca ccttcttata gaacccaacc
      421 gcctgctcat tttgctcatt aacgttggtt gtcagttccg gtgccatcga gagcgcatgc
      481 tccaccagca tccgacccac gccgcagccg cacacatcag gatcgataaa cagcgcatcc
      541 atatgctgcc cacttaacaa cataaaccca accggctgat cccgttcatt aaccgcgacc
      601 cacaacggcg cttccggcag gaaggaacga actagcacct ccagctcggc ccgatactct
      661 gctgataaaa aatcgtgtgt ggcatcgaca gaacgacacc aaatcgcaac gagttcctcc
      721 ccttcctcat gccgtgagcg gcgaatacta ataaccattt tctctccttt tcgtcattct
      781 tatattctaa cgtagccttt tccttgaaac tttctcacct tcaacatgca ggctcgacat
      841 tggcaaattt tctggttatc ttcagctatc tggatgtcta aacgtataag cgtatgtagt
      901 gaggtaatca ggttatgccg attcgtgtgc cggacgagct acccgccgtc aatttcttgc
      961 gtgaagaaaa cgtctttgtg atgacaactt ctcgtgcgtc tggtcaggaa attcgtccac
     1021 ttaaggttct gatccttaac ctgatgccga agaagattga aactgaaaat cagtttctgc
     1081 gcctgctttc aaactcacct ttgcaggtcg atattcagct gttgcgcatc gattcccgtg
     1141 aatcgcgcaa cacgcccgca gagcatctga acaacttcta ctgtaacttt gaagatattc
     1201 aggaacaaaa ctttgacggt ctgatagtta ccggcgcgcc gctgggcctg gtggagttta
     1261 atgatgtcgc ttactggccg cagatcaaac aggtgctgga gtggtcgaaa gatcacgtca
     1321 cctcgacgct gtttgtctgc tgggcggtac aggccgcgct caatatcctc tacggcattc
     1381 ctaagcaaac tcgcaccgac aaactctctg gcgtttacga gcatcatatt ctccatcctc
     1441 atgcgcttct gacgcgtggc tttgatgatt cattcctggc accgcattcg cgctatgctg
     1501 actttccggc agcgttgatt cgtgattaca ccgatctgga aattctggca gagacggaag
     1561 aaggggatgc atatctgttt gccagtaaag ataagcgcat tgcctttgtg acgggccatc
     1621 ccgaatatga tgcgcaaacg ctggcgcagg aatatttccg cgatgtggaa gccggactag
     1681 acccggatgt accgtataac tatttcccgc acaatgatcc gcaaaacaaa ccgcgcgcga
     1741 gctggcgtag tcacggtaat ttgctgttta ccaactggct caactattac gtctaccaga
     1801 tcacgccata cgatctacgg cacatgaatc caacgctgga ttaatcttct gtgatagtcg
     1861 atcgttaagc gattcagcac cttacctcag gcaccttcgg gtgccttttt tatttccgaa
     1921 acgcacctca gcaagtgaat aaattttatt cacattgtta tcaataagtt atcaagtatt
     1981 tttaattaaa atggaaattg tttttgattt tgcattttaa atgagtagtc ttagttgtgc
     2041 tgaacgaaaa gcgcacaacg atccttcgtt cacagtgggg aagttttcgg atccatgacg
     2101 aggagctgca cgatgactga acaggcaaca acaaccgatg aactggcttt cacaaggccg
     2161 tatggcgagc aggagaagca aattcttact gccgaagcgg tagaatttct gactgagctg
     2221 gtgacgcatt ttacgccaca acgcaataaa cttctggcag cgcgcattca gcagcagcaa
     2281 gatattgata acggaacgtt gcctgatttt atttcggaaa cagcttccat tcgcgatgct
     2341 gattggaaaa ttcgcgggat tcctgcggac ttagaagacc gccgcgtaga gataactggc
     2401 ccggtagagc gcaagatggt gatcaacgcg ctcaacgcca atgtgaaagt ctttatggcc
     2461 gatttcgaag attcactggc cccggactgg aacaaagtga tcgacgggca aattaacctg
     2521 cgtgatgcag ttaacggcac catcagctat accaatgaag caggcaaaat ttatcagctc
     2581 aagcccaatc cagcggtttt gatttgtcgg gtacgcggtc tacacttgcc ggaaaaacat
     2641 gtcacctggc gtggtgaggc aatccccggt agcctgtttg attttgcgct ctatttcttc
     2701 cacaactatc aggcactgtt ggcaaagggc agcggtccct atttctatct gccgaaaacc
     2761 cagtcctggc aggaagcggc ctggtggagc gaagtcttca gctatgcaga agatcgcttt
     2821 aatctgccgc gcggcgccat caaggcgacg ttgctgattg aaacgctgcc cgccgtgttc
     2881 cagatggacg aaatccttca cgcgctgcgc gaccatattg ttggtctgaa ctgcggtcgc
     2941 tgggattaca tcttcagcta tatcaaaacg ttgaaaaact atcccgatcg tgtcctgcca
     3001 gacagacagg ccgtgacgat ggataaacca ttcctgaatg cttactcacg cctgctgatt
     3061 aaaacctgcc ataaacgcgg tgcttttgcg atgggcggca tggcagcgtt tattccgagc
     3121 aaagatgaag agcgtaataa ccaggtgctc aacaaagtaa aagcggataa agcgctggaa
     3181 gtcaataacg gtcacgatgg cacatggatc gctcacccag gccttgcgga cacggcaatg
     3241 gcggtattca acgacattct cggctcccgt aaaaatcagc ttgaagtatt gcgcgaacaa
     3301 gacgcgccga ttactgccga tcagctgctg gcaccttgtg acggtgaacg caccgaagaa
     3361 ggtatgcgcg ccaacattcg cgtggctgtg cagtacatcg aagcgtggat ctccggcaac
     3421 ggctgcgtgc cgatttatgg cctgatggaa gatgcggcga cggctgaaat ttcccgtacc
     3481 tcgatctggc agtggatcca tcatcaaaaa acgttgagca atggcaaacc ggtgaccaaa
     3541 gccttgttcc gctagatgct gggcgaagag atgaaagtca ttgccagcga actgggcgaa
     3601 gaacgtttct cccagggccg ttttgacgat gccgcacgct tgatggaaca gatcaccact
     3661 tccgatgagt taattgattt cctgaccctg ccaggctacc gcctgttagc gtaaaccacc
     3721 acataactat ggagcatctg cacatgaaaa cccgtacaca acaaattgaa gaattacaga
     3781 aagagtggac tcaaccgcgt tgggaaggca ttactcgccc atacagtgcg gaagatgtgg
     3841 tgaaattacg cggttcagtc aatcctgaat gcacgctggc gcaactgggc gcagcgaaaa
     3901 tgtggcgtct gctgcacggt gagtcgaaaa aaggctacat caacagcctc ggtgcactga
     3961 ctggcggtca ggcgctgcaa caggcgaaag cgggtattga agcagtctat ctgtcgggat
     4021 ggcaggtagc ggcggacgct aacctggcgg ccagcatgta tccggatcag tcgctctatc
     4081 cggcaaactc ggtgccagct gtggtggagc ggatcaacaa caccttccgt cgtgccgatc
     4141 agatccaatg gtccgcgggc attgagccgg gcgatccgcg ctatgtcgat tacttcctgc
     4201 cgatcgttgc cgatgcggaa gccggttttg gcggtgtcct gaatgccttt gaactgatga
     4261 aagcgatgat tgaagccggt gcagcggcag ttcacttcga agatcagctg gcgtcagtga
     4321 agaaatgcgg tcacatgggc ggcaaagttt tggtgccaac tcaagaagct attcagaaac
     4381 tggtcgcggc gcgtctggca gctgacgtga cgggcgttcc aaccctactg gttgctcgta
     4441 ccgatgccga tgcagcggat ctgatcacct ccgattgcga cccgtatgac agcgaattta
     4501 ttaccggcga gcgtaccagt gaaggcttct tccgtactca tgcgggcatt gagcaagcga
     4561 tcagccgtgg cctggcgtat gcgccatatg ctgacctggt ctggtgtgaa acctccacgc
     4621 cggatctgga actggcgcgt cgctttgcac aagctatcca cgcgaaatat ccgggcaaac
     4681 tgctggctta taactgctcg ccgtcgttca actggcagaa aaacctcgac gacaaaacta
     4741 ttgccagctt ccagcagcag ctgtcggata tgggctacaa gttccagttc atcaccctgg
     4801 caggtatcca cagcatgtgg ttcaacatgt ttgacctggc aaacgcctat gcctagggcg
     4861 agggtatgaa gcactacgtt gagaaagtgc agcagccgga atttgccgct ataccttcgt
     4921 atctcaccag caggaagtgg gtacaggtta cttcgataaa gtgacgacta ttattcaggg
     4981 cggcacgtct tcagtcaccg cgctgaccgg ctccactgaa gaatcgcagt tctaagcaac
     5041 cgacggtaat gactccaact tattgatagt gttttatgtt cagataatgc ccgatgactt
     5101 tgtcatgcag ctccaccgat tttgagaacg acagcgactt ccgtcccagc cgtgccaggt
     5161 gctg
//
LOCUS       DAETQN010000210         5114 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.213, whole genome shotgun sequence.
ACCESSION   DAETQN010000210 DAETQN010000000
VERSION     DAETQN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.213"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..379
                     /locus_tag="K0W68_004214"
     CDS             <1..379
                     /locus_tag="K0W68_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075601.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="K0W68_004215"
     CDS             complement(575..2125)
                     /locus_tag="K0W68_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBI3075602.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="K0W68_004216"
     CDS             2297..2743
                     /locus_tag="K0W68_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HBI3075603.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="K0W68_004217"
     CDS             complement(2937..3872)
                     /locus_tag="K0W68_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBI3075604.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4384..>5114
                     /locus_tag="K0W68_004218"
     CDS             4384..>5114
                     /locus_tag="K0W68_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075605.1"
                     /translation="MGKMNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMT
                     VGKAFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSI
                     AQNNNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYL
                     DRMLYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKN
                     ASPLITKNFPGFYERVPPCENADEC"
BASE COUNT         1417 a         1244 c         1127 g         1326 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attgcaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgccgga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcagag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa ggagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 gcagatgaat gcat
//
LOCUS       DAETQN010000211         5044 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.214, whole genome shotgun sequence.
ACCESSION   DAETQN010000211 DAETQN010000000
VERSION     DAETQN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.214"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            73..1601
                     /gene="lsrA"
                     /locus_tag="K0W68_004219"
                     /pseudo
     CDS             73..1601
                     /gene="lsrA"
                     /locus_tag="K0W68_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1595..1903
                     /locus_tag="K0W68_004220"
     CDS             1595..1903
                     /locus_tag="K0W68_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3075606.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLMRKVVSAMISWIILSRARQKYGAASVVVDEQSWLSSGVKKSPY
                     "
     gene            complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="K0W68_004221"
     CDS             complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="K0W68_004221"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBI3075607.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3750..4700)
                     /locus_tag="K0W68_004222"
     CDS             complement(3750..4700)
                     /locus_tag="K0W68_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBI3075608.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4839..>5044)
                     /locus_tag="K0W68_004223"
     CDS             complement(4839..>5044)
                     /locus_tag="K0W68_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HBI3075609.1"
                     /translation="ALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSL
                     SEEQQRYIVAVIYHWLVVQMNQP"
BASE COUNT         1316 a         1288 c         1230 g         1210 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgtcgattt tacgttgcat caaggggagg
      181 tccacgccct gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg
      241 gtattacccc tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa
      301 cgccagttca tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc
      361 caagcctgtc gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc
      421 agaaaatgaa gaacttgctg gcggcgctgg gctgctagtt tgatctgcat agtctggcag
      481 gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct ccgcggcctg atgcgcgact
      541 cgcggattct gatcctcgat gaacctaccg cctcgcttac ccctgcggaa accgaacgct
      601 tgtttcgtcg cttgcaagag ctgcttgcta ctggcgtggg tattgttttt atctcgcata
      661 agctgccgga aattcgccag attgccgatc gaattagcgt gatgcgcgac ggaaccatcg
      721 ccttaagcgg caaaaccagc gaactgtcta ccgatgacat tattcaggcc atcacgccag
      781 tggtacggga aaaatcgctc tctgccagcc aaaaattatg gctggagtta cctggcaacc
      841 gcccacaaca tgccgccgga acgtcggtgc tgacactaga aaatctgacc ggcgaaggtt
      901 tcaggaatgt cagcctgacg ctcaatgccg gagaaattct gggtctggcc gggctggtgg
      961 gggccggacg cacagaactg gccgagacgc tctatggtct gcgtactttg cgtggcggac
     1021 gcattatgct gaatggtaaa gagatcaata aattatccac tggagaacgt ttactgcgcg
     1081 gtctggttta tctgccggaa gatcgccagt catccggact gaatctcgat gcttcgctgg
     1141 cctggaacgt ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa accgcgaaag
     1201 ataatgccac cctggaacgt tatcgtcggg cgctgaatat taaatttaac caaccggaac
     1261 aagctgcacg gacattatcc ggtggcaacc agcaaaaaat cctgattgcc aaatgcctgg
     1321 aagcttcgcc gcaagcattg attgtcgatg agccgacgcg cggcgtggat gtctcggccc
     1381 gtaatgatat ctaccagctg ttgcgcagca tcgccgcaca gaatgtggca gtgctgctta
     1441 tctcctctga tctggaagag atcgaactga tggcagatcg tgtgtatgtg atgcatcagg
     1501 gcgaaattac ccactctgca ctgaccgggc gcgatattaa tgtcgagact attatgcgcg
     1561 ttgccttcgg cgatagtcag cgtcaggagg cgtcatgctg aagtttattc agaacaaccg
     1621 tgaaatcacg gcactgctgg cggtggtgct gctgtttgta ttacccggtt ttctcgaccg
     1681 ccagtattta agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct
     1741 gctggcaatg ggcgcgacgc tggtaatgct tatgcgcaaa gtagtaagcg cgatgatatc
     1801 ttggataata ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga
     1861 acaatcatgg ttatcttcag gcgtgaaaaa gtcgccctat taaacgtgat tacgatcaca
     1921 ttctcaagac gctacttaca aattacctac aagcttacaa cattaacagg cgctgcatgt
     1981 ggcatctgtt aatgataaag gcacatagga tgttgtaact aatatagtga aataagaaac
     2041 ccggtcaaaa ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc
     2101 gcatcccttt ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca
     2161 cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct
     2221 gggtaccgat aacatggtca cgatgctggc tccagagttg ctgataacgt tccagccagt
     2281 gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag aacgcaatca
     2341 atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca
     2401 gcagctgcgg caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca
     2461 gctgatgctt atacgggtta cggaaacgcc cggtgactgc actggcgaaa gattccagtt
     2521 catcacgagg caaatccagt accggaataa tttcttcgta gatggctttt tcaacgaatg
     2581 cgcaaatttc agcatcgttc atcgcttcac ctacggtatc cagccctgcc tggaacgcca
     2641 ccggcaccag cgcggtgtgc gcaccgttga ggattgccac tttgcgttct ttatacggtt
     2701 taatatcgtc aacaatcagc acgttgagcg gatatttgtc caggcgcagc tcagtcgcta
     2761 aggatttcgg cccctgaatc acaaacaggt aaaagtgttc agcggtgtcg aggaaaccat
     2821 cgtgataacc cagttcttct tccagtttag ccacttcatc gcgcggataa ccggtaacga
     2881 tacggtccac cagcgtagaa cagaagctgt tagcctgatc cagccattga ataaatgctt
     2941 ctggcagtgc ccactcttgc gcatagcgca gcaccagttc acgcaacgcg tcgccattgt
     3001 agtcgatcaa ctcacacggg atgatgatcc aacctttatc cagcgcaccg ttgaaatggc
     3061 taaagcgttc gaacagcaga cgggtcagtt ttgccggata gcttatcgct ggcgcgtcat
     3121 cgaatttatc gcccgcatgg tagctgatcc ccgcttcggt ggtgttagag aaaacaaagc
     3181 gcatttccgg gttgtgcgcc agtttcagga attcatcgta ttcactgtag acgctgattt
     3241 cacgattaac cgagcgaatc agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc
     3301 cacggataat ggtggtatac agaccatcct gcgtgctcag tgacggcggg aatgaagttt
     3361 caatcggacg aacaacgacc acgccagaat tcagatcggt gtgctcattc aggagatcga
     3421 tttgccagtc aacaaaggcg cgcaggaagt taccttcacc aaactgaatg atacgttctg
     3481 gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt tcccttctga
     3541 ttagtcatac aacctgtttg aattggtacg acaggttagc aaactttaat acgccgaacc
     3601 cctgttttga tcaactcctg atgattaatg agcagtttta tgagaaaagt gtggcgcgga
     3661 tcatggttta atcgaggaaa tacgcctttt cctggatcat aaagtggtag aacacattgc
     3721 attcaaatcg cgcggaatga ataaagatgt cagacaactt cctcaccgta acgcatagtg
     3781 ctggtacggt tgcgcccatc tttcttcgag cgatacagac acgtatcggc ttcgaccatc
     3841 aatttattaa aatcatcggt cagcgttcgg tacgatgcgc gaccactacc gacgccaata
     3901 cttaccgtga gataaagcgt tttttgttgc caggtgaatg gttgcagttc aacgccttta
     3961 cggatttttt ccgccattag cagaccatcg acaggattca ccgacggcac tgcaacagca
     4021 aattcttcgc cgcccatgcg cgccaccagc cccttatcac cgacaatctt ctgaatatgc
     4081 cgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc gttaatgctt
     4141 ttgaaatagt cgatatcaag cagcatgacc gtcagaggca cgatcaacac gcccgttgag
     4201 aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc aatactgacc
     4261 gaaccaatcg ccccgacaat agcgcataat aatgcggcaa tcagacagta attaaatagc
     4321 cgtaaggcac ttactggctc atacttattt ttccccagac gcttatcccg cacgaccagt
     4381 aaggcgacgg taacaataaa catcatattg gagaaattga taaccagtga aaccagtccc
     4441 cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc atagtaatgc
     4501 agtcgattaa gccagacata gcgggcaaac ccccccgcca ttacgccatt cagcggccag
     4561 aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt
     4621 agcgcaaaaa tagcgatgct attacgtagc agatgacctt cctggaacaa acggaaagtt
     4681 gaaatggggt gtacatgcat taattatctg tccatacaat tgcgggtggc aattatctca
     4741 acgtaataag taaattctca taaaggatag catgttcgct aacagcggcg gcaaataaag
     4801 gtaccctggg aatgctattt tatcgttttc cgcaattctc atggctgatt catctgtaca
     4861 accagccagt gatagattac cgccacgata taacgttgtt gttcttcgct taacgaaaca
     4921 ccctgcggaa tgttatgcca cttagccaga caaccgcgac agcaggtcgc ggtggcgtgc
     4981 tgagcgataa acaccggatg cccgcgcatg ggggtttgct taccgtcatt agccggtaat
     5041 gccg
//
LOCUS       DAETQN010000212         4937 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.215, whole genome shotgun sequence.
ACCESSION   DAETQN010000212 DAETQN010000000
VERSION     DAETQN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.215"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..279
                     /locus_tag="K0W68_004224"
     CDS             <1..279
                     /locus_tag="K0W68_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3075610.1"
                     /translation="YTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWK
                     NRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA"
     gene            620..2272
                     /locus_tag="K0W68_004225"
     CDS             620..2272
                     /locus_tag="K0W68_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBI3075611.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIREHSPLDAASYEPKGEVSWQ"
     gene            complement(2283..2453)
                     /locus_tag="K0W68_004226"
                     /pseudo
     CDS             complement(2283..2453)
                     /locus_tag="K0W68_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2586..2705)
                     /locus_tag="K0W68_004227"
                     /pseudo
     CDS             complement(2586..2705)
                     /locus_tag="K0W68_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2779..3213)
                     /locus_tag="K0W68_004228"
     CDS             complement(2779..3213)
                     /locus_tag="K0W68_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HBI3075612.1"
                     /translation="MDTFKRRNVATSFSFLGYDFKVRTLKNFKGELYRKCMPGASNAA
                     MCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFSYRLWSAMQSR
                     LLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYLLRASNE"
     gene            complement(3390..4699)
                     /gene="ltrA"
                     /locus_tag="K0W68_004229"
                     /pseudo
     CDS             complement(3390..4699)
                     /gene="ltrA"
                     /locus_tag="K0W68_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(4763..4891)
                     /locus_tag="K0W68_004230"
                     /pseudo
     CDS             complement(4763..4891)
                     /locus_tag="K0W68_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         1319 a         1194 c         1145 g         1279 t
ORIGIN      
        1 tacacacagc ggcttaagga agccggatta ctggcatcaa caggaagtac aggcgactcg
       61 tatgacaacg cgatggcgga gagcatcaat ggtctttaca aagcggaggt aatacaccgt
      121 aagagctgga aaaaccgtgc agaagtggaa ctggccacac tcacgtgggt ggactggtat
      181 aataatcgac gattgctgga aaggctgggc cacatccctc cggcagaagc agaaaaagct
      241 tattatgctt ctatcggaaa cgatgatctg gcagcctgag ttcacagata aaacattctc
      301 caggaaactt ggggcggttc acgtcagtat cagaccatat taattgctga aactgataaa
      361 catcaaacct tatagaaggg tgaatgctga aagggggcta tggtgatcga cgtgatgaca
      421 acgacaggta agaccgtgac ctgataaacc tgaattgtgt cctagacata aagtccgtga
      481 agggaataag gctcaggtca gcgaatctta tagtggcccg cagctctgac tgcaataagg
      541 ccggatacag agcagcaact actcgtcgat gtcagaacaa cggaagcctt gcaaacgggg
      601 atgcgttcat acaggtacaa tgataccgtt aaaaaaaaac attacgctaa taatgttcac
      661 cctgtcatta ctgacaggaa atcctgctat agcgtatgaa acagataaag tatataaaat
      721 aaccgttctg catacaaatg accaccatgg acacttttgg cgtaacaatc atggtgagta
      781 cgggttatcc tcccaaaaaa ctctggtgga caatatccgg cagaaagtaa taaataatgg
      841 cggaagtgtt ctattattat ctggtggtga tattaacacc ggtgtacctg aatcagacct
      901 tcagaaggct gaacccgata ttcgtggaat gaatctaatt ggttatgatg caatggccgt
      961 cggtaatcat gaatttgata atcctctaaa tatattacgt cagcaggaaa aatgggccac
     1021 atttcctttc ctttctgcta acatatatca aaaaagtaca gggaggcgtt tattcagtcc
     1081 atggaaaatt ttcatccgtc agaatttgaa aattgcagtc attggcttga cgacagatga
     1141 tactgcgaaa acaggaaatt ctgaatattt tactgatatt gagttccgtc aacctgctgc
     1201 tgaggcgagg agtgtcattg atgaacttaa tcagcaggaa aagccagata ttattattgc
     1261 ggcaacacat atgggtcact atgacaatgg cgagtcaggt tcgaatgctc ctggtgatgt
     1321 tgaaatggct cgcagtttgc cgactggctc attagcaatg attgtgggag gacattcaca
     1381 agctcctgtc tgcatggcca gcgataataa aaaacagtgg aattatattc cggggacaac
     1441 ctgtgtacca gataaacaaa atggcatctg gattgtacaa gcgcatgagt ggggaaaata
     1501 tgtcggtcag gctgattttg aattctgtaa cggtacaatg aaactggtta attatcagtt
     1561 acacccagtt aatctcaaaa tgagaattac cagagaagat ggaaaaacgg aattttcttt
     1621 ttacaccccc gaaattactg aagatccaca gatgttatcc ttactgacac cattccagaa
     1681 taaaggcaaa gcacaactgg atgtgaaggt tggggttgtg aacggacgtc tggaagggga
     1741 cagaagtaaa gttcgttttg ttcagaccag tatgggccat ctgattctga gcgctctgac
     1801 agaacgtatt gacgcagatt ttgctgttgt cagtggtggg gaaattcgag attccataga
     1861 gagtggtaac atcacataca aagatatact gaaagttcag ccatttggta atacggtagt
     1921 aagtattgat ttgacgggga aagaagtagc tgactaccta gctacagtgg cacagatgaa
     1981 accggattcc ggagcctacc cacaattttt gaataccagc tttgtagtca aaaaaggaaa
     2041 aatcgaaatg ctaaaaataa aaggtaaatc ggttgacctt aacaaaaaat atcgtatgac
     2101 aacatttagc tttaatgcta ctggtgggga tggctatcca cgtattgata atagacccgg
     2161 atacataaat accggattta ttgatgctga agtacttatc gaatatatcc gggaacattc
     2221 accgctggat gctgcttcat atgaaccaaa aggtgaagtc agttggcagt gataacgtaa
     2281 ttgacgatca tcacgtgttt tttgctgaca gtcgctgtga ctttgtgaaa gtagtctctg
     2341 cggatgtttg cgatatggga atgcagttgc tgtattttgt gtttctgctt ctgccagttg
     2401 ttgctgaatt taaccttgcg gcttaactga tgttggagcg ctgccagctt cttcgtcaat
     2461 ttcgctgatt aaagtttatt cattatggca tgatctgcgt aagcggctcg tcagaaccgt
     2521 attgatattt actaagagct cagatcaact ttccagggca acagatcgcg tacccggttt
     2581 gccggtcagt cctggatatg ctcaatgacg taacgcagcc atttttctgg ctccacattg
     2641 ttcagacggc atgtgccgat cagcgagtac aacaccgccg catgttcgcc accgctatcg
     2701 gaaccccgcc ggagagagtt tcactctccg gctgctcacg gaaccgtacg tgacactctc
     2761 gcgtcatacg gctcttgatc actcattcga tgcacgtagt aaataccagt gggcaaatag
     2821 cttcggatac tgtttcctta ccagcgccag ctttcgctga gccttccggt tcgaaagtct
     2881 gtatttagac tgcatccact taagcaaacg tgactgcatt gcactccaca gtcgatagct
     2941 gaagtttctg gaccagaact ttccatagta ctcaatccag cctctcacta tcgcattgta
     3001 gcgcctcgca aaatccagca aactctcagc tgttgagcga tgtatacgcc acttcttgat
     3061 tgtttctgtt attttgcaca ttgctgcatt tgatgcaccc ggcatacatt ttctgtacaa
     3121 ttcgcctttg aaattcttca gagtacgcac tttgaagtca tatccgagaa aggagaaact
     3181 cgtagcgacg tttctccttt taaacgtgtc aatgtaggcg atattcgtct tccctgcgtt
     3241 caggaccaac ccgacttccg agaagcgcac cgataacctg ttcttcagcc ttgttgcatc
     3301 actcattcgt gaacaatgta ctacaagtcg agtcacccga gggaacctcc ccctctggtg
     3361 ctcacggaac cggacgtgaa cctctcagct catccggctc ttctcattga ggcctgtccc
     3421 catagtaacg gccagtgtgc aaacaacctc gtttcactcc gcgcaacacg ggccagccag
     3481 tggctggccc gtcgtcggtg gcccctaagt cgcttgtgtt tacggggcag ccataacacg
     3541 agtttgcgat cgatttgcct cagcgtcaga tacagtgctg atttataaaa agcaccgtag
     3601 tactttatcc agcctctgat tttcgcattg aacatatgcg ccagctcgta caaggacttg
     3661 tcactgcgat tctcctcacc tcctgacgga ttgcttttcc tgctctggca ctcatcgccg
     3721 gactgaagtt aacgaaggtc ttaccccagc gattcatcga cagacgcggc cggaatgtat
     3781 agcccagaaa gtcgaagctc gtttccgggt agtccccccg tcggtcagca tccttacagt
     3841 aaactattcg tgttttttgc ggatgcagct gcagcccgca ttgcgcaagc cgctgcctca
     3901 gtccacttat cagagcgtca gcctgccact ggctgtgaca gtggcataca acatcatcgg
     3961 cgtacctttc gaagggaacg ccgggaaact gacgttgcat ccacatatca aaggcatagt
     4021 gcaggaacag atttgagagc aggggggaga ttacgccgcc ctgcggcgtt cctcgacccc
     4081 gtgccagcat tgctccatcc ggcatttgca ccggtgcctt cagcctgcgc tcaatgtaca
     4141 tcaccaccca gcgtgcctga gtatgatgct gcactgtctt cagtagcagg tcatgatcga
     4201 tatcatcaaa gaagccttta atatcgactt ccaccaccca gtcaaacttc cagcagcgtt
     4261 tcctcgcctg cgcaatcgcc tggtgtgcgg atttacccgg tcgataacca tacgagtctg
     4321 catggaacag gggttccagt accggctcga gtatctgctt aacgaccatc tgggcgatcc
     4381 ggtccgacac ggccggaata ccgagcggac gtacaccacc gtcagctttg ggaatgtcca
     4441 cacgccttac cgcctgcggc atataactgc cggaagccat tctgttccac agtttgtaga
     4501 gattgtctgt aacattctcg tcaaacccgg ccagtgtctg cccgtcaatg cctgccgccc
     4561 cacggttggc ttttactcgt ttataagctt cccatacctg tcgtttactt acaggaaatg
     4621 gctttgccga ttttacagca gtcctcccat cggcattgtc gcccgtggtt gccgccgcac
     4681 catcagcaca atggggcaag ccctttgctc cggacccatt acggtccttc accgctacta
     4741 cggtttactc cgtccctgta ccatttttca gcgttcccca gaagctttcc atcggagcgt
     4801 tgtcgtaaca gttaccttta cgcgacattg atgttttcag accaaactgc tcctgtatga
     4861 cccggtaatc gtatgcgcag tactgtgaac ctcaaaaagt tatcggtgcg tttatcgaaa
     4921 aacatatgtt ctactaa
//
LOCUS       DAETQN010000213         4882 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.216, whole genome shotgun sequence.
ACCESSION   DAETQN010000213 DAETQN010000000
VERSION     DAETQN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.216"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(358..1401)
                     /locus_tag="K0W68_004231"
     CDS             complement(358..1401)
                     /locus_tag="K0W68_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBI3075613.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1813..2997)
                     /locus_tag="K0W68_004232"
     CDS             complement(1813..2997)
                     /locus_tag="K0W68_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3075614.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(3297..3391)
                     /locus_tag="K0W68_004233"
     tRNA            complement(3297..3391)
                     /locus_tag="K0W68_004233"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3355..3357),aa:Sec,seq:tca)
     gene            3684..>4882
                     /locus_tag="K0W68_004234"
     CDS             3684..>4882
                     /locus_tag="K0W68_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HBI3075615.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
BASE COUNT         1276 a         1069 c         1117 g         1420 t
ORIGIN      
        1 ccacgggaat atctgcggca gcgggcttgt aatgggttaa gtgataacag atgtctggaa
       61 atataggggc aaatccacgg ggataccagt tcaaccgaaa acgccagagg aggggattac
      121 ccgctggcag ggtaaatctg tggtttttga tgccagctat aacgtgtttg acgataaagt
      181 attacgaaag aactttaatg agttcatgga gtaattcgtt aaagtaattt acaacgctat
      241 ccttttgcaa aggaaacttt cagaaaataa ccccagggta atcacctggg gaaggaacac
      301 agtgttatta ttggataata aattattact tgttttaaag ttacactaat gcaatatcta
      361 ctgatactgg atgcttgaat gtttcatccc tgctgcggaa aagggcaata tccagggcca
      421 gatatattca ccgttcatat ttctgcattt ctgtacaatt tcctcagaaa gtgtcttgtc
      481 aattcttttc cacaggtgct cctgctgtct taaaatactt tcgcatattt cgaaatcatc
      541 ttcctgaatc agcatttcgg cgcatttaat gatttctgat cgttcatgca gcacggagga
      601 atggttatag agccagttta caaacagcaa tgcttccgga tattgtcccc ataatggtga
      661 gtatgttgca tagtaatctt ctgccgtatc cagagagccg gagaagtcat gccacacagc
      721 aagtggaaat ggatgcagat ttccgcacag catttctgcg atgtaagtgt ttgtggcaaa
      781 gccatggcga aaagctgtgg cggccttaac atgttccccg ttcagaaaat gcaggagacc
      841 gagttcatac cagtaaggtg aaaattcatc agcatgctct ttcagtacac tgaaggctcg
      901 ctcatgatcg ccggtgcgta acagctctga acccaacaac cagcgagccc cctgattatc
      961 ttcaggattg tatgccagta ttttatcagt gagcataacc gcatcctgat ggcgctgtaa
     1021 gtgcacattt gcgagaattg ctgcataaag cgcgcgcaga taaggccggt tttcgggatg
     1081 catccagatt atttcaccac agaaactttc cgggatgata cgattacctg ccgccagtcc
     1141 tttaagagag gcgttcagtg ccttacgcgg ggcattctgt tccagaaatg catatgccag
     1201 atgagcgtgt atatcaataa agtccggctc ccgttgagca aggcgtcgga gtttgttgat
     1261 gtaactcgtc tcgctgattt catcagcgtc gtatgcatca agcacttctt caaactcccc
     1321 cagcaatggg ccaaaccagg tatccggaaa tacgaaataa cccttgttat cgtgcacttc
     1381 aaagcataat ctatcgttca tttctgagcc tgtaaaacat gtggtgatat agtctgagag
     1441 cataaaagtt gagattctgt gtatcaagtc ctgtcttggc accgaatgaa tattgcttac
     1501 gaagcccctt catgctttcc ttgagaacaa taattttatc tttatgtgaa tctcattttt
     1561 tataatgatg tcagatgatt gaaatgctaa attatatctg aatcaacttt tcctttgcta
     1621 aaggtatcga aataactgca aggctgtatt ttgtaattgc caatttaaaa atgtcaattt
     1681 gtttttaatt atgtttttgt aataagtttg aagacgcaaa aaaaataata taataacaaa
     1741 aatcctccag ccttgtaggc cagaggataa aatacttaat tgaggaaaat atcagggaaa
     1801 agtatttctg acttaacttt cccggctgga gagtgaatca atgtaatcag cgtaccattg
     1861 catcatttca cggcggccat cgagatacag ggcatgatta taggtacccc tgatggaatt
     1921 tttatccaca tgagctaact gcatttcaat ccaggcggaa ttaaagcctt gttcatgcag
     1981 aattgtactc atggtgtgtc tgaaaccgtg ccctgtgagt cttcccttat atccaagtaa
     2041 ttcaattact tggttaatgc tttccttact aattggtttg cggggatcat ttctaccgat
     2101 aaagactagg ggatgatgtt ttgagattgg ttcaagctgc ttaaatagca taatgacctg
     2161 ctcagagagc ggcacaatat gaggtcgctt catcttcatt acttctgccg ggatctccca
     2221 caattttgtc tcaaaatcga tatcttccca gcgtgcaaaa cgcagttctt gtgttcgtac
     2281 gccggttaac ataatgatct gagtagctgt cttagtgatt atacttccgg tataacccgc
     2341 cagatcgttg aggaagtggg gaagttcatt agcagtaaga aagggaaaat gtactttttt
     2401 cggtgtagct aaagcactgg caagatcggg ggctgggtta tagtctgccc ggccagtaac
     2461 aattgcatag cggaacacct caccacagcg ctggcggacc ttgcgcattt tctccagagc
     2521 tccacgtttt tccattttac gcagtgcttc cagtaactcc attggtttga tttcggcaat
     2581 agggcgcttg ccaatgtagg gaaaaatgtc tttttcaaag gtatcaatga tttcatcacg
     2641 atagcgtaat gaccaccgat cagcttttga ctggtgccat tcgcgagcaa tagcctcgaa
     2701 cgtatttttc agactcattt gctgcgcgat tttttcttcc ttttttgcct cccctggatc
     2761 gcctccagca gcgaggagtt tcctggcttc actgcgtttc tcgcgagcat cggctagcgt
     2821 tacatcgggg tatacgccaa tagaaagcat cttttcttta ccagcaaagc ggtacttcat
     2881 gcgccagtat ttagaaccat tagtgttaat cagtagatac ataccgccgc catcagcaag
     2941 cttatagggt ttgtctttgg gttttgcggt tttcactttg atatcggtta atgccatgac
     3001 atcctccggc ttttgatgaa ttcatgttcc tgagggggat ttctgccagt aactaacctg
     3061 atacccccaa tacacccctt gtataacaaa gatattgtta aaagccgttg ttatgtatca
     3121 catttttcct attatggggg tgcttgtatt tcaataggaa ataacacccc ctaaagtacc
     3181 cccaaaagaa acttgatatg gggtgaagta ggttgacctc aggatacaaa taaatactga
     3241 tatacaaggg attagaagag atttattgac attgggagag ttcagaagag gtaaactggc
     3301 ggaagatcac aggagtcgaa cctgcccggg accgctggcg gccccaactg gatttgaagt
     3361 ccagccgcct caccggagac gacgatcttc cgcgcctcga ttgctacatg gaggcggggc
     3421 gcattatagc tacttccttg agtttctaca tcccccagat cagttttttc cgcctaatac
     3481 acgcagcgta ttatcacgaa agcattataa ctcatataaa acccatattt tacaatgata
     3541 tatgagcgga attaacgcta ccacgatcac ataaaacaag aatcataaat taataaccag
     3601 atatcggaat attcgctctc acagggatgg tttacaaaat gcgttatcgg tctcagcacc
     3661 cctatagcat caaggaaaag cagatgaaga gtgaagtgtt gtccgttaaa gagaaaattg
     3721 gttatggcat gggagacgcc gccagccaca ttattttcga taacgtaatg ttatatatga
     3781 tgttctttta taccgatatt tttggcattc ctgccggatt tgtcggaacc atgtttttgg
     3841 tcgctcgtgc actggatgcg atttccgatc cttgcatggg gttgttggcc gatcgaacgc
     3901 gctctcgctg gggtaaattt cgtccgtggg tactgtttgg cgcactgcca ttcgggatcg
     3961 tctgtgtact ggcctatagc acgccagatc tcagtatgaa cggcaaaatg atctatgcag
     4021 caattactta caccctactt accttacttt ataccgtcgt caatatccct tactgcgcat
     4081 tgggtggtgt aatcaccaat gacccgactc agcgtatctc gctgcaatcc tggcgttttg
     4141 tgctggcgac cgcgggaggc atgctttcta ctgttctgat gatgccactg gttaatttaa
     4201 ttggcggtga taataaacca ctcggtttcc agggcggtat cgcggtcctt tccgtggtgg
     4261 cattcatgat gctggcattt tgtttcttca ccactaaaga acgcgttgaa gcaccaccta
     4321 caacaacgtc tatgcgggaa gatttacgtg atatctggca aaacgaccag tggcggattg
     4381 tcggtttact aaccattttc aatatcctgg cggtgtgcgt acgcggtggg gcgatgatgt
     4441 attacgtcac atggattttg ggcacgccgg aagtgtttgt cgcttttctc accacttatt
     4501 gcgtgggtaa cctgattggt tccgcactgg caaaacctct gaccgactgg aaatgtaaag
     4561 tcactatctt ctggtggacg aacgccctgc tggcagtgat tagcctcgcg atgttctttg
     4621 ttcccatgca ggccagcatc actatgtttg tcttcatctt cgtgattggt gtgttgcatc
     4681 aactggtgac acctatccag tgggtaatga tgtccgatac cgtcgactac ggcgagtggt
     4741 gcaatggtaa acgcctgacc gggatcagtt ttgctggcac gctgtttgtg ctcaaactgg
     4801 ggttggcctt cggcggcgct cttatcggct ggatgctggc ttatggcgga tatgatgcgg
     4861 cagaaaaagc gcagaacagc gc
//
LOCUS       DAETQN010000214         4838 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.217, whole genome shotgun sequence.
ACCESSION   DAETQN010000214 DAETQN010000000
VERSION     DAETQN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.217"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(263..463)
                     /locus_tag="K0W68_004235"
     CDS             complement(263..463)
                     /locus_tag="K0W68_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075616.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(463..735)
                     /locus_tag="K0W68_004236"
     CDS             complement(463..735)
                     /locus_tag="K0W68_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBI3075617.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(738..1016)
                     /locus_tag="K0W68_004237"
     CDS             complement(738..1016)
                     /locus_tag="K0W68_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075618.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(1017..1574)
                     /locus_tag="K0W68_004238"
     CDS             complement(1017..1574)
                     /locus_tag="K0W68_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075619.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
     gene            complement(3138..3689)
                     /locus_tag="K0W68_004239"
     CDS             complement(3138..3689)
                     /locus_tag="K0W68_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3075620.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            4488..4784
                     /locus_tag="K0W68_004240"
     CDS             4488..4784
                     /locus_tag="K0W68_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBI3075621.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT         1364 a          993 c          921 g         1560 t
ORIGIN      
        1 aattgataca ccggaatcag cgaaagcctg caccatcata gcagtgtcgc tatcaatgaa
       61 aatctgtaat tcatattcgt tcatttttaa aatctccttt ttaccagacc tcctcaatcg
      121 ctctgtaacg agctaaacag tctttttcag gggtagcctg tacagtggta ttacccaccc
      181 taaaataaga tagttttttg cgttagaatg cgattgagag cgtctgataa gctgtttttc
      241 taatttctct taaagtctcg gactaccaat aacctaattc cgctttattt cgttcaaacc
      301 attccgctgt aactgctgtc tttagtgttg cgtttgggct ggtggcatga atgaagatat
      361 cttttggatt gcaagcatca ccgaaaccac cttctttgat gagcaggtct gatacatgct
      421 taatcatttt atctgtcaaa tattcgaatt taatacataa cattaaatct ccaggagtaa
      481 attgtttggg tggcaatttc ttttattacc atccctgaag ataatctttt tagttgcctg
      541 aacttcatga tgcaaaaacc agatgacata cttcagataa taatcacgag tgccataacg
      601 aatcattgga ttaccgtaag catcatctct tgtcttaacc tctccaccat caatccattc
      661 aagcattcct gtctcataat taatcatgaa gtaagttgca aagaaccgtt taatatcatc
      721 tcgccattgc ttcataatca gacctcctga gacagtttgt gaattttgat acttagtaac
      781 cttgctaaat ccagtccaaa agcatttaag gatttcagac tatcaaggtc atcttgctta
      841 atcctgatat ggtgtcttgc agcctctaag cgactattaa gcaaatcctg ttcgttaata
      901 tcacggttaa ttgcaagctt catcactgct gagtgtggca gacctgtaat gtttttgatt
      961 cggttcagtt catcaaaggc tgtctttgta actctggtta atgtgtgttt attcatttaa
     1021 aaatcctctg taactaaata ggggtcaaac tcgaaatctg gttgctcgtt ttccattggc
     1081 tcaatattcc caacggcaat atgatgttca acaatgccaa ttaatttcgc gcacatcaca
     1141 gcaagcaaag cgcaatattt atcgcttggt aattcgtaac taagtctggt tgcctgtcgc
     1201 tgaagatgat ttgtcacggt ggatgcgtta atttcgtcaa gattctttag cagataaaat
     1261 agttttttgt tgtatttcgg gtggccttca cttctacaga acccaataaa aacatcatca
     1321 agcttgcttg gtaactgctc gtaactgtca aagttagcca gataattttt aatagtattc
     1381 tttgttgttt tgcgtactgg cataaatctg tctcgtggcg taatgctacg aatgaccgct
     1441 tcaatgttct tacgttcgga tttgctaaag gttgggactt cgtagctggt ggggttgttt
     1501 gtgtaagcgt aggagttgtg cttgttgcgt tctcttgatg cctgaagcat cgctctaaat
     1561 tctgaatact gcattcatgt tctgactctc ataagatgtt attattctgt taacctatat
     1621 acaatattag cattttcaaa gactaatgtc aagtattttt aacattttcg taacatctgt
     1681 ggtataatgg gtttatgggg agagtaaaat atgaggcgtg gctatgtatt tggtcgcata
     1741 ttttgcattg cacacagacc tcttcaatcg ccctgtaacg agttaaacga cctcttcagg
     1801 tcaactcata aggtcaggta ctttttgtgt cagaatgcga ttgagagcgt cttacaagga
     1861 aaactaatgg taaatctccc tcctgagctg gggtcacctg atgtatggac tggtggaggt
     1921 attgggctga ttacaggcct gctacttcgc tacgtcctcg tcagagtgct tgttgtcttt
     1981 aaactgttcg gtcgtacatg gacgcttgac cttaaggata acgaccacac agacccccgt
     2041 taaactcctt caaaacccct gcacgagatg acagatagaa gtctatggta caggggtgtc
     2101 tttagcattt tgtgctatct attaaaaaaa tagcttgaca gaaccagaaa gccgtatcta
     2161 tcaagcattc acctctctga cagtaatttc taaaagttct tatttttcaa taagttgtaa
     2221 ttagccatat atagagaact aaaagatttt ttagctattt tgtttaaaag catatcaaac
     2281 gatacatttt attgatttag gtcaaagaca aaagcattga caaaatggta agattgttca
     2341 catgacctta cttaacagac ttttaagaga ctttgaaaca gcctgtttaa caggtcacta
     2401 cctctggtct tatcgttccc ttctttctag gtggttatct gttaagagtt tttcagtttt
     2461 tattcttaag ataattgtta agatgatact ctcccttcgg gttatatcgt atctcttctc
     2521 ccgttaaata tcccttcaca gtcagactaa ataacacctg tgtagttctt ggtagaatct
     2581 ttcaaggtaa cttagctctg agtttatgaa gttgacctat gagatagact ctaccgaatc
     2641 tgttagtcaa ttgttgctta ggtgctgcta aggcttactt agtggaaaga gaggggatat
     2701 gtgaccatag gggaacccct cctgttaaaa gactacttag aagtctgagg ttttagtaaa
     2761 atctgtgaat atggcttctc agacgctctg taacgcattc taagcgattt taccccttta
     2821 ttttaaggtg gttagtacat ccctataggt tacccctaaa aatggggtta aaactcgcta
     2881 cagagctttc tatgagatta gcttctaatc gaatcaacta tgaggtttgg taggattgtg
     2941 caaaatctgt acagtaactt ttcagaatct gaaaattttt ataaaaaatg actagccctc
     3001 tttgaatctc atcttccccg aatcttcaaa aatatctgac agatgtctgt agctgctaag
     3061 tacccgtcac accagctatg tgaccccctt ggcaaaaaag ccaaatatca gctacatcca
     3121 tgtaactatt cagtaactta tttagcaagt ctgtaataag ttgctctaga gattcctaac
     3181 tcttcctgaa tctgcaacct cgtcttacct tgtgaaataa gcgtctgtac agcctctgaa
     3241 gaagctttta caggcctccc catatgtacc ccttcagctt tagcacgttc aataccagcc
     3301 tttctacgct ctgctatcaa gcttctttca agctgtgcca acgttgacat aatgcctaac
     3361 attgcttcac ccatagcgct tgataagtca aacccttctc taaggctgat aactgtcaca
     3421 cctttagcct gtagcgcttt aaccgttgat aacacatcaa ttgtgtcacg tccaagacgg
     3481 tctacagcta ccacaactac ggtgtcacct tctcgtgcat aagctaatag tctgctgaaa
     3541 ccgtttctta aagaagcttt gacagaacca ctgacagctt catcagtgaa ccatttagca
     3601 atgttgtacc ctgcctcttc aatttgcatt tgctggttag tgatggattg gtcatctgta
     3661 ctcaccctgc aatatccaag tatcgccata atgttcccct tagaagtctc aaaagttagt
     3721 ctcataagtt tagctgtctc ataatccaaa aacaaccttt tgagactact tgagggtgca
     3781 ttctaccgtt acgttgaaag tgtctcaaaa ggaagtcctt ttaagactgg ttatgaagga
     3841 gctatgaggt tgattagggg ttggtacaag cccaactaac cgaatcaact atgaagattt
     3901 taggagggta actgtaacca tcactttcta actttcacgt aggaaattgt taagtggttt
     3961 gatagttaca ccatgtgacc aagcatctat atatcgttat atagccctgt atatagcact
     4021 atatgtagtg tcgagagagg aacgactaac acgatacagt ttaccctgaa aagagacttc
     4081 acagattctt tatatatttc aataagttag ggtaaaaagg gactataccg agactgtata
     4141 gaatctgtgt agaattgcat agatacttgg aaatctacta ttaaattgca tagggactca
     4201 cctgatattg tcctgttttt aacaggtctc ctagaaaacc gaatagactt cattcaaggg
     4261 tcattgaagt cataatcatt tcagtagaaa aaccaagtct cgattttaaa tgcaaatagc
     4321 aatcatattc aataaggcaa gacgatcagg atcagtgaaa atgtcatttt tggcgtaatt
     4381 gaccttactt tatgcatggt cttaacaaag acatcatctt tccgttgcag atcttcgccc
     4441 tttcaaataa cgtactttac aactttcccg aacaaggagt tgtgcccgtg ttgtatgtta
     4501 tttacgctca agacaaagct gatagcctcg aaaaacgcct ttccgttcgt ccggcacatt
     4561 tagcacgttt acagttactg catgatgaag gtcgtttgtt gacagctggt ccaatgccag
     4621 cggtagacag taacgatcca ggggctgcgg gatttactgg ctcaaccgtt attgctgaat
     4681 ttgaatcgct ggaagcagct caggcctggg ccgatgctga cccgtacgta gcggcaggtg
     4741 tctatgaaca cgtttcagtg aaaccattta agaaagtgtt ctgaatagag attcaatatt
     4801 taagggcacg gtttttgcaa ccgtgttctt gtttggcc
//
LOCUS       DAETQN010000215         4784 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.218, whole genome shotgun sequence.
ACCESSION   DAETQN010000215 DAETQN010000000
VERSION     DAETQN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.218"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            6..81
                     /locus_tag="K0W68_004241"
     tRNA            6..81
                     /locus_tag="K0W68_004241"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="K0W68_004242"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="K0W68_004242"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="K0W68_004243"
     CDS             complement(299..1057)
                     /locus_tag="K0W68_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBI3075622.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="K0W68_004244"
     CDS             complement(1065..2168)
                     /locus_tag="K0W68_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBI3075623.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="K0W68_004245"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="K0W68_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4452)
                     /locus_tag="K0W68_004246"
     CDS             complement(3427..4452)
                     /locus_tag="K0W68_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3075624.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1311 a         1236 c         1143 g         1094 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccttcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggcg acgttgagtt cggtatctgt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 atcagtgacg ccagtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cctcccctga ctgtaaagcg gtgaagcgtt cttttgcagt
     4201 gagcggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aacatctaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctgt acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccatc ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aggtgtgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc gatataacat gcta
//
LOCUS       DAETQN010000216         4699 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.219, whole genome shotgun sequence.
ACCESSION   DAETQN010000216 DAETQN010000000
VERSION     DAETQN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.219"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            387..1469
                     /locus_tag="K0W68_004247"
     CDS             387..1469
                     /locus_tag="K0W68_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HBI3075625.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="K0W68_004248"
     CDS             complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="K0W68_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HBI3075626.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(2127..2267)
                     /locus_tag="K0W68_004249"
     CDS             complement(2127..2267)
                     /locus_tag="K0W68_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HBI3075627.1"
                     /translation="MMFEFYMAEFLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="K0W68_004250"
     CDS             complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="K0W68_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HBI3075628.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2623..2913)
                     /locus_tag="K0W68_004251"
     CDS             complement(2623..2913)
                     /locus_tag="K0W68_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBI3075629.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2906..3076)
                     /locus_tag="K0W68_004252"
     CDS             complement(2906..3076)
                     /locus_tag="K0W68_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075630.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3076..3531)
                     /locus_tag="K0W68_004253"
     CDS             complement(3076..3531)
                     /locus_tag="K0W68_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HBI3075631.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3796..4173
                     /locus_tag="K0W68_004254"
     CDS             3796..4173
                     /locus_tag="K0W68_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075632.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
BASE COUNT         1351 a         1048 c          977 g         1323 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagtcaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta
      301 catcttgaaa taatcacatt gattagatga atatttatcg cgcaatgaca tcatttttta
      361 ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      421 ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta
      481 acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct aatgatgcag
      541 atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc
      601 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag
      661 gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
      721 ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc
      781 tgccagaatt cggtggtgac acttggactc aaacagatgt gttcatgact ggtcgcacca
      841 ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      901 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt
      961 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     1021 ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     1081 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     1141 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     1201 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1261 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata
     1321 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1381 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1441 ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc
     1501 tttgcctccc caacatataa gtggctccct caagccactt cctttaggag cacaaccttg
     1561 cttctaacta tataaacctt ctgttatata ttacccttta tttttggggg cgttgcaacg
     1621 ccccattttt aataattttt agtaaacaat tggcatatta attagagtta ttaacaacga
     1681 tatccatctc taaccggata tctaatgcca ttaacatccc ttcaattatg ccctcagcct
     1741 tctgtaacct tttcccgata taaccatcag agcagcaatg cttacctgcc agtgacatga
     1801 atgtcatacc gactacataa taatctacta ataaatcgtg caaatcgctg ttgttctttt
     1861 tcagacgggc catgcacccg caaataatca tcgcgtcatc gtcacaacat tgcgggcgag
     1921 attttacttt tgaagtaatt aatcccttaa aaccggcggc aatggacgac caggtcacat
     1981 cttcatgatt attagccgcc cacgctcccc aacgctcaag aaccatctga atatcacgca
     2041 tcaacttact ccacaaaaat cagaccagaa cgccaattac aagcaaaaat caacaaaaca
     2101 gtattagttg attgttatct ctgacttcat actcctgctc ctgtcagggt tttggcgtaa
     2161 ttcttcagta ttcggtaatc ggtcaaaaca gaaccgggga aacgatataa gcgcagatgc
     2221 ccccagcggt ggcgaagaaa ttctgccata taaaactcaa acatcattca ttccccattt
     2281 cggtgatggt cagttccagc ctcccacctt tggtaacagg catcttcaca acgcggtaat
     2341 caacgacctg agcatcatcc agccagaaac ctgctttggt gagtgcgtca aaagcggctt
     2401 tttgcagatt atccaggtca cggcgacggc gatccggcat gtggcactca atacggattt
     2461 tcactggcat agccaggccg atatccagca ttgcgttttt aatgattcgg gtgacgttat
     2521 cgcggtatgc ctgcccttct gcgctgatgt gtgtgcgccc gcgattatgg cggtaatagc
     2581 ggttattgct cggaggccag ggtaatgtga tgctgtaggt attcacgcct taataacccc
     2641 ctctttcagc cagataacct gcgttctcgc cataccttcc agcgcgcatt cttttgcata
     2701 ctcagcatcg acaaaatatg tgcggcggtc gatttcgtcg tgacaggcag aacatgcaat
     2761 ggtggcaatc aggtctggcg gtttgatacc ggtaccgcac aatccagcca gccggatatg
     2821 tgccagtact gacgtttcag gattgccatt acatacgcca gggattctta cctggcattc
     2881 ccgaccacgc gctgcttttc tcaaatcagc catgattcct ccttgctgcc agtcgcaacc
     2941 attttttatc aaccaggcta gcggtatatc cgagcagtgt tggtatttcg gatggcttca
     3001 gctcaggctt acgcttacga cgatttgata ctctgtagat gtgcccgttc atgacacgaa
     3061 taagcggtgt agccattacg cctcctgctt atcgcgcagc tgctggtatt cacagctctg
     3121 cggaattgtc aggtggcagc cgatattcat cgcccaggct tcgactttgc acaggaagat
     3181 gtacatctcg ccggtttcca gctctgacgt atggcggagg gattggacgg tggtgacctc
     3241 gccggacacg acatctacac ggtctttgct ttcgtagccg agataggtgt gcttcatcgc
     3301 gtctttgacc cactcaggcg tagcgaaggt cttgccgcgg gcgatgagat attcgctgat
     3361 ctccgtgtac cacatgtggc tgagcgcgtt ctgcgacagg ctgcgcttct cgcgccacgg
     3421 cttaacctgc aggcgtaagc attgcccggc atccagcaat ggctgaatct gctggccgat
     3481 ggccgcgaag ttaccgcgat ggagtttgat gccgtctact ggcagagtca tacggcctcc
     3541 ctaacggaaa ccgcagaatg cagaaaatcg caggtgcatt tctgcatctg tgacaaggtg
     3601 atgagttcag attgtggtcg catttaagtc cccttaaatg cgcagaagtc accggagttg
     3661 ttcaggctcc gatgagagga ttatggcgga ttgattacga taaatcaaaa ctatcttgaa
     3721 gccaaaacag tgatcttcgc tttaaaaacc ttgtatactt tcacctaatc acagccagaa
     3781 ctatggatct aaccaatgac caacaaagaa aacaaagata aatatattaa caagttcaag
     3841 aaagagcttt caaaatatat acaaccaact gctggagtgg acattcaatt atttaactct
     3901 aaggatggtg gtggtgtaat taaagccact ttgaatagaa gtggaaaaag aaaatcatct
     3961 attgctggta attttactaa attaggtgag gcaattacct cgagtgggca gagggttttt
     4021 ggtggtgatc tatcaaatgt gaatttttac ggaacaaata cgatttttga tggagataca
     4081 atatttttaa ttaaatcacc tgattcagag gagtggtcat cacaaaaagc ttccaaagac
     4141 gtagagggca tcgtatttgg agggaaaaaa tgattaacat tactactaag gatgcactca
     4201 agtcagtggt gaagtaagag atctagtaat tcttcttgaa actgatggaa aaacggtgtc
     4261 tgattatttg cagccaatcc ctcccaaaaa agatacacac tggagcatga ttttcattcc
     4321 ggtagcaatt tatatactac ttaatcttgt atacattatt gctcacccat tatttcctga
     4381 aaaatatcta aaaacatcaa catctatatt tctattcatt tccttcatat taagtgcaat
     4441 agtagcatat tttgttttcc ataagcataa atctgccact ctatccatat gcagcttagt
     4501 gttcttagtg ttaattgtag gtgctaacgt agggtttatc agttacagcg acctaacaca
     4561 aaaagcgacg caaaaattag atagtttgtc taaataatta tgttgccatg cgaagtatgc
     4621 atggctgcat gtctgccttc catttaaaat gggctaagac ctataaccct aaatattatt
     4681 ctttattatc ttctttacc
//
LOCUS       DAETQN010000217         4665 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.220, whole genome shotgun sequence.
ACCESSION   DAETQN010000217 DAETQN010000000
VERSION     DAETQN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.220"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..154
                     /locus_tag="K0W68_004255"
     CDS             <1..154
                     /locus_tag="K0W68_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBI3075633.1"
                     /translation="QMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDISL
                     LRYMPA"
     gene            complement(546..608)
                     /locus_tag="K0W68_004256"
                     /pseudo
     CDS             complement(546..608)
                     /locus_tag="K0W68_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1059..2471
                     /gene="ospC1"
                     /locus_tag="K0W68_004257"
     CDS             1059..2471
                     /gene="ospC1"
                     /locus_tag="K0W68_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HBI3075634.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSIANQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2575..2724)
                     /locus_tag="K0W68_004258"
                     /pseudo
     CDS             complement(2575..2724)
                     /locus_tag="K0W68_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2800..4497
                     /gene="ospD3"
                     /locus_tag="K0W68_004259"
     CDS             2800..4497
                     /gene="ospD3"
                     /locus_tag="K0W68_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HBI3075635.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1620 a          825 c          891 g         1329 t
ORIGIN      
        1 acaaatggtg aaacagttcc tgagtcgtga cccgttcgaa tgcgtgctgt gtggcggccg
       61 gatggtatac cgccgggcca ttgcggggct gaatgtggag gggctgaaga aaaacgcgcg
      121 ggatatcagt ctgctgaggt atatgccggc ctgacacagg ctaagtgtgc ccgggaatcg
      181 gtgcaagtat aaaaaacagc ctgttgcggt ataagaatcg accatgcaga gagagaaaac
      241 accggagtgg tggggaaaaa cagaaaggct cacgtggcat aaggcgcgga caggggtatc
      301 gcttagtttt tcaatttcct atacgtgaac gcccgcctgc gggcactgtt gcagacgcaa
      361 caggacacta tccaccagat ggctgaatac aaccgcctgc tctcacagcg gatggcggct
      421 tatgcttccg aaatcaaccg actgaaggcg ctggttgcga aactgcagcg tatgtagttc
      481 ggtaaaagct cagaaaaact tcgcgcaaaa accgaacggc agattccatt ttcacgggcg
      541 atctacgcca cacaggctct gggttgcagt gactgctcca ttaaggcgat tttgaactca
      601 taagggaagt aacaaaaaaa ctgcaaaagt acaataatta tatatggcca aactaacctc
      661 tattaaaaaa acatcagttt ttcacactta gttgagcttc atgaaaaaat catcgaggat
      721 ttcattgcag aatacacatt ccactaactg gaagcactcc ataagaggaa tcagagaaag
      781 tatcgttttt ttatagtaaa attcattgct gttcaataaa attgatatag ttattaaatt
      841 atgaaaataa tttcacaaaa aagattaact tgagttctta agcatcggat tttagatatt
      901 ttgaaatatc tggcatcaca taacatcaaa agtatataac acccttatct aattacatgc
      961 agtatattgt atatatactc aaaaattgta attcataccc tgctatatct tgtagggaaa
     1021 gatattaatt aaaactgttt tcatataagg ttcattttat gaatatatca gaaacactga
     1081 actcagcaaa tacccaatgc aatatagatt ctatggataa cagattacat acattgtttc
     1141 caaaagtgac atcagtgcga aacgctgcac aacaaactat gccagatgaa aaaaatttaa
     1201 aagatagtgc aaatattatt aaagatttct ttaggaaaac tatagcagca cagagttata
     1261 gtagaatgtt ctctcaaggc tctaacttta aatctttaaa tatagcaatt gatgcaccat
     1321 cagacgctaa agcctcattt aaggctattg agcaccttga cagattatcg aagcattata
     1381 tatctgaaat aagggaaaaa cttcatcctc tttctgcaga ggaactcaat ttgctttcgc
     1441 taattattaa ttctgattta atcttcagac atcaaagtaa ttctgatttg tctgataaaa
     1501 ttttaaacat taagtcattc aataaaattc agtctgaagg aatatgcaca aaacgaaaca
     1561 catacgctga tgatataaaa aaaatagcta atcatgactt tgtgtttttt ggcgttgaaa
     1621 tctctagcca tcagaaaaaa caccccctga atacaaaaca tcacactgtt gattttggtg
     1681 caaatgcgta tatcattgat catgactctc catatggata tatgacatta accgatcact
     1741 ttgataatgc tattccacct gttttttacc atgagcacca atcattttta gataaatttt
     1801 cagaggttaa taaagaagtt agtcgatacg tacatggaag taaaggaatt atagatgtac
     1861 caatattcaa tactaaagat atgaagttag ggctcggatt atacctgatt gactttatta
     1921 gaaaaagtga agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt gcccctgtgg
     1981 atctggatag aatcataaac tttgtttttc agccagagta ccatatacct aggatggtaa
     2041 gtacagaaaa cttcaaaaaa gttaagatta gagaaatatc cttagaggag gctgttacag
     2101 catctaatta cgaagaaatt aacaagcagg tcactaacaa aaaaattgct ctccaggctc
     2161 tttttctttc gattgctaat caaaaagagg atgtcgcctt atatatatta tctaattttg
     2221 agataactag acaagatgtt atttccataa agcatgagtt gtatgatatt gagtatctac
     2281 ttagcgctca taattcaagc tgtaaagtac ttgagtattt tatcaataag ggattggttg
     2341 atgtaaacac aaagttcaaa aaaactaata gtggggattg tatgttggat aacgcaataa
     2401 aatatgagaa tgcagaaatg ataaaactat tattgaaata tggtgcaaca tctgacaata
     2461 aatatattta atcaaaattg aatatcgttt agcaaaaggc agtatttgca caaaatatcg
     2521 atatattgtt tattgtcagt atggctcaat gtgataatag ttggaaagtt tgatgggttt
     2581 cgccccgttg tagcggtagt cgagctggaa ggtcttcagg cactgcttac agcgatagag
     2641 cagcccccct gaactggaat ggccgttccg gatacccccc tgagtttcag agtaacgggg
     2701 acaaaccaca tcaatctttg ccatcaatca tccaaagggc aaagagtaca acaacactaa
     2761 gtctgcgtca caacccatca atgaaaggaa tatatacata tgccatcagt aaatttaatt
     2821 ccatcaagga aaatatgttt gcaaaatatg ataaataaag acaacgtctc tgttgagaca
     2881 atccagtctc tattgcactc aaaacaattg ccatattttt ctgacaagag gagtttttta
     2941 ttaaatctaa attgccaagt taccgatcac tctggaagac ttattgtctg tcgacattta
     3001 gcttcctact ggatagcaca gtttaacaaa agtagtggtc acgtggatta tcatcacttt
     3061 gcttttccgg atgaaattaa aaattatgtt tcagtgagtg aagaagaaaa ggctattaat
     3121 gtgcctgcta ttatttattt tgttgaaaac ggttcatggg gagatattat tttttatatt
     3181 ttcaatgaaa tgatttttca ttccgaaaaa agcagagcac tagaaataag tacatcaaat
     3241 cacaatatgg cattaggctt gaagattaaa gaaactaaaa atggggggga ttttgtcatt
     3301 cagctttatg atcccaacca tacagcaact catttacgag cagagtttaa caaatttaac
     3361 ttagctaaaa taaaaaaact gactgtagat aattttcttg atgaaaaaca tcagaaatgt
     3421 tatggtctta tatccgacgg tatgtctata tttgtggaca gacatactcc aacaagcatg
     3481 tcctccataa tcagatggcc taataattta cttcacccca aagttattta tcacgcgatg
     3541 cgtatgggat tgactgagct aatccaaaaa gtaacaagag tcgtacaact atctgacctt
     3601 tcagacaata cgttagaatt acttttggca gccaaaaatg acgatggttt gtcaggattg
     3661 cttttagctt tacaaaatgg gcattcagat acaatcttag catacggaga actcctggaa
     3721 acttctggac ttaaccttga taaaacggta gaactactaa ctgcggaagg aatgggagga
     3781 cgaatatcgg gtttatccca agcacttcaa aatgggcatg cagaaactat caaaacatac
     3841 ggaaggcttc tcaagaagag agcaataaat atcgaataca ataagctgaa aaatttgctg
     3901 accgcttatt attatgatga agtacacaga cagatacccg gactaatgtt tgctcttcaa
     3961 aatggacatg cagatgctat acgcgcatac ggtgagctca ttcttagccc ccctctcctc
     4021 aactcagagg atattgtaaa tttgctggcc tcaaggagat atgacaatgt tcccggactt
     4081 ctgttagcat tgaataatgg acaggctgat gcaatcttag cttatggtga tatcttgaat
     4141 gaggcaaaac ttaacttgga taaaaaagca gagctgttag aagcgaaaga ttctaatggt
     4201 ttatctggat tgtttgtagc cttgcataat ggatgtgtag aaacaattat tgcttatggg
     4261 aaaatacttc acactgcaga ccttactcca catcaggcat caaaattact ggcagcagaa
     4321 ggcccaaatg gggtatctgg attaattata gcttttcaaa ataggaattt tgaggcaata
     4381 aaaacttata tggaaataat aaaaaatgaa aatattacac ctgaagaaat agcagaacac
     4441 ttggacaaaa aaaatggaag tgattttctg gaaattatga agaatataaa aagctgaata
     4501 ttatctcgtg gtgatacact gaatttgagg taaccggctc atttaaaccg tctggtctgt
     4561 tacctccggt tccacaaaaa taatgtccat cattttgaag tggacactat cgtatggaat
     4621 accggacctg gatcactgaa gctttacgcc ttcatttcga agaac
//
LOCUS       DAETQN010000218         4607 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.221, whole genome shotgun sequence.
ACCESSION   DAETQN010000218 DAETQN010000000
VERSION     DAETQN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.221"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..2163
                     /locus_tag="K0W68_004260"
     CDS             <1..2163
                     /locus_tag="K0W68_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBI3075636.1"
                     /translation="KGTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKA
                     SGMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMR
                     EMMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGK
                     PAPYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLS
                     SVWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVY
                     EIDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYEN
                     VIAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCF
                     GAGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSAS
                     ELDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKV
                     CSKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLA
                     GNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLA
                     NIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVI
                     HEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2397..3680)
                     /locus_tag="K0W68_004261"
     CDS             complement(2397..3680)
                     /locus_tag="K0W68_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBI3075637.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3815..4507)
                     /locus_tag="K0W68_004262"
     CDS             complement(3815..4507)
                     /locus_tag="K0W68_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3075638.1"
                     /translation="MLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTKPVAATRA
                     AKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGY
                     LYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVS
                     RYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRD
                     DRGREWDKIEIK"
BASE COUNT         1094 a         1142 c         1194 g         1177 t
ORIGIN      
        1 aaaggcacta ttgcctggtc tattctctct tctcataata cgtccggaaa tatggataaa
       61 cttaaaatta agtttgattc attagcctct cacgatatta cctttgttgg tattgtacag
      121 accgctaaag cgtccggaat ggaacgtttc ccgctgccgt atgtgctgac caactgccat
      181 aactcactct gcgccgtcgg cggcaccatt aacggtgatg accatgtttt tggtttatcg
      241 gcagctcagc gttatggcgg tatttttgtg cctccgcata ttgcggtcat ccatcaatat
      301 atgcgtgaga tgatggcagg cgggggcaaa atgatcctcg ggtcagacag ccacacccgt
      361 tacggtgcat tagggacaat ggcagtcggt gagggtggcg gtgagttggt aaaacagctg
      421 cttaatgaca cctgggatat cgactatccg ggcgtggttg cggtgcatct gaccggaaaa
      481 cctgcgccgt atgtggggcc acaggatgtg gcgctggcta tcattggcgc ggtgttcaaa
      541 aacggttacg tcaaaaacaa agtcatggag ttcgttggac cgggcgttag cgcgctctct
      601 accgatttcc gtaacagcgt tgacgtgatg accacggaaa ccacctgttt aagctccgtc
      661 tggcaaactg atgaagaagt gcataactgg ctggcgctgc acggtcgcgg ccaggattac
      721 tgccagctta accctcaacc gatggcgtac tacgatggct gcatcagcgt tgatttaagc
      781 gccatcaaac caatgattgc gctgccgttc cacccgagca acgtgtatga aatcgacaca
      841 ctgaaccaga acctgaccga cattctgcgt gagattgaaa ttgagtccga acgcgtggcg
      901 cacggtaaag ccaaactctc gctgctggat aaagtggaaa atggtcgcct gaaagtgcag
      961 caggggatta tcgcgggctg ttctggcggt aactacgaaa acgtcatcgc ggcggcgaat
     1021 gcactgcgcg gtcaatcctg tggcaatgac accttctcgc tggcagttta cccgtcatca
     1081 cagccggtgt ttatggatct cgccaaaaaa ggtgtggtag cagatttgat tggcgcaggc
     1141 gcaatcatca gaaccgcgtt ctgcggccca tgctttggcg cgggcgatac gccaatcaac
     1201 aacggtttga gtattcgcca caccacgcgt aacttcccga accgcgaagg ctctaagcca
     1261 gctaatgggc agatgtcagc ggtggcgttg atggacgctc gttctatcgc tgcgactgcg
     1321 gcaaacggtg gctatttaac ctctgccagc gaacttgatt gctgggacaa cgtgccggag
     1381 tacgccttcg atgtaacgcc gtataaaaac cgtgtttatc agggctttgt gaaaggggca
     1441 actcagcaac cgctgattta cgggccgaac attaaagact ggccggaatt gggtgcgctg
     1501 actgacaata tcgtcctgaa agtgtgctcg aagatcctcg acgaagtgac caccaccgac
     1561 gaactgattc cttccggtga aacctcttct tatcgttcaa atccgattgg tctggcggag
     1621 tttaccctgt ctcgccgtga tcccggttat gttggcagaa gtaaagcgac tgctgagctg
     1681 gaaaatcagc gtctggcggg gaatgtcagc gagctgacag aggtgtttgc gcgcattaag
     1741 cagattgctg gtcaggagca tattgatccg ctgcaaactg aaattggcag catggtctat
     1801 gcggtgaaac caggcgatgg ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt
     1861 ggcggtctgg cgaatattgc cgaggagtac gcgactaaac gctaccgttc taacgtcatc
     1921 aactggggga tgttacctct gcagatggcg gaagtgccaa cctttgaagt gggggattac
     1981 atttacatcc ctggcattaa agcggcgctg gataatccgg gtacgacgtt taaaggttat
     2041 gtgatccatg aagatgcgcc ggtaacggaa attacgctct atatggaaag tctgactgct
     2101 gaagagcgcg agattatcaa ggcgggtagt ttgattaact tcaataaaaa ccgtcagatg
     2161 taaaaagcgc catgtgaatg taggtcgcat tcggcactta ttgtcggatg cgatgcttgc
     2221 gcatcttatc cgacctacga atcgcatcga atctgtaggc cagataaggc atttacgcag
     2281 catccggcac ttattgtcgg atgcgatgct tgcgcatctt atccgaccta caaatcgcat
     2341 cgaaccgtag gccggataag gcgtttacgc cgcatccggc aaatagttaa ttgctcttac
     2401 ttcttcgcct ctgcaaccac tttactaccc acgccgcggt tattgtattc ccacatgcgg
     2461 ttgtagttag tgtcattcag attgcgctgt atttcgtcgt tatcatctac gctgccggta
     2521 ttacccgcaa acggacgatt agagatcacc gcatcggccc acggtttagc cgtgttaaaa
     2581 ccttcgttga tggcgctatc acggatcacc acctgaccgt tggtattggc atcaacatcc
     2641 agcgagcggc ccagttgcgc cacaccatca ccgaaagcat tgaaacggct gtttacggcg
     2701 aggaaaccgt agtacatgtt ggacagcgta gccggtgcaa acacatacgc ttcttgctga
     2761 gtacgtgagt tcaccacgcg gaattcggtg ttatcgaaca ccactgcgcc gcgaccagaa
     2821 acgatatcca catccccttc aatgtagctg ttggtcacca gcgtacgcgg ctgacgattc
     2881 gtttccagac ggttctgcac accgctgttg gtgacaaaga aggtgttctg acgaccgaga
     2941 atgttaacgt tgttaatctg tacctggtca ccatcagtac gcagtgccac cgccggatgg
     3001 ttacctgcat ctacgctatc gcccagcgtg ttttcgatgg tcagattttg cagttgcagg
     3061 ccattgtttt gtgaccagaa gaccgcagag cagagaacac cgatactgtc gctgcgtttg
     3121 ctctggcagc tatcgtacat ataccacgct ggtttacctg gcatatattt gccgcgcggg
     3181 ttgacgtcgt gacgccagtc ggcagggctc atgccaccat caagggaaag cccaatcttc
     3241 acatcaatcg gtttttcacc tgtaccgtac agagtaattc cacccggagc ggcagggaca
     3301 tataccgttc cctgatactc accaggcatc acggcaatat actggcgctt gttggtacgc
     3361 ttgataattg ccgcatctac cgccgcctga atcgtggtat gcgttacacc ttgagtgccc
     3421 gccgggccga caacaaagtc aggttgcgca ggcagggtaa tcggggaagg attccacgct
     3481 gcagcacctg gtgtcaggga tgcaaaatag tgttgagcat cgaaattctg cgcttctttt
     3541 gccgacagaa tcgggcgaga agaggtacca ggcgcggttt gatcagaagg acgttgatcg
     3601 ggcggggttg agctacaggc ggtcagcgtc acgccaaaag ccaatgccag cgccagacgg
     3661 gaaactgaaa atgtgttcac aggttgctcc gggctatgaa atagaaaaat gaatccgttg
     3721 aagcctgctt ttttatacta agttggcatt ataaaaaagc atcgcttatc aatttgttgc
     3781 aacgaacagg tcattatcag tcaaaataaa atcattattt gatttcaatt ttgtcccact
     3841 ccctgcctct gtcatcacga tactgtgatg ccatggtgtc cgacttgtgc ccgagaagat
     3901 gttgagcaaa cttatcgctt atctgcttct catagagtct tgcagacaaa ctgcgcaact
     3961 cgtgaaaggt aggtggatcc ccttcgaagg aaagacctga tgcttttcgt gcgcgcataa
     4021 aataccttga tactgtgccg gatgaaagcg gttcacgacg agtagatgca attatggttt
     4081 ctccgccaag aatctctttg catttatcaa gtgtttcctt cattgatatc ccgagagcat
     4141 caacatgcaa tgttgtaggg atggcaattt ttacgcctgt tttgctttgc tcgacataaa
     4201 gatatccatc tacgatatca gaccacttca tttcgcataa atcaccaact cgttgcccgg
     4261 taacaacagc cagttccatt gcaagtctga gccaacatgg tgatgattct gctgtttgat
     4321 aaattttcag gtattcgtca gccgtaagtc ttgatctcct tacctctgat tttgctgcgc
     4381 gagtggcagc gaccggtttt gttgttatat ggccttcagc tattgcctct cggaatgcat
     4441 cgctcagtgt tgatctgatt aacttggctg atgccgcctt gccctcgtct atgtatccat
     4501 tgagcattgc cgcaatttct tttgtggtga tgtcttcaag tggagcatca ggcagccccc
     4561 tccttattgc tttaattttg ctcatgtaat ttatgagtgt cttctgc
//
LOCUS       DAETQN010000219         4482 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.222, whole genome shotgun sequence.
ACCESSION   DAETQN010000219 DAETQN010000000
VERSION     DAETQN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.222"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(7..1068)
                     /locus_tag="K0W68_004263"
     CDS             complement(7..1068)
                     /locus_tag="K0W68_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBI3075639.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1065..2462)
                     /locus_tag="K0W68_004264"
     CDS             complement(1065..2462)
                     /locus_tag="K0W68_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBI3075640.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2617..3615
                     /locus_tag="K0W68_004265"
     CDS             2617..3615
                     /locus_tag="K0W68_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI3075641.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="K0W68_004266"
     CDS             complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="K0W68_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HBI3075642.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1229 c         1098 g         1063 t
ORIGIN      
        1 attgcattat ttttcgctgg gtgttttgcc tttttgcagt gtttctttca cctgaccgat
       61 aagctgacga cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg
      121 gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga agtcctgcac caatcccctg
      181 agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc
      241 gcgcaccagt tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg
      301 caaacggctg taatacccca gttcaatgcc atacagcgtg gcgatgcgat taattaaacg
      361 cagattgcgc caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat
      421 caacgttgat tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg
      481 ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga
      541 ggcataccag cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt
      601 ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc
      661 gcgttcatgg gcgcgctgtc gcaagcacca taagcactgc cactctgtta ccacagaacc
      721 tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg
      781 ggtttgccag gcattcattg tccactgtac accctgcccg acaacacttg cgccaaacag
      841 agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc
      901 cattaccgct tcaacttgcc cctcttcttc aggcgcttca tcgagcgtgg ccggggcaaa
      961 attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgatcgac
     1021 ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
     1081 cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc
     1141 atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg
     1201 ggcaaacgcg caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccga
     1261 ttaccgcgca atgccggaat tttctcgccg ttgacatcaa taatgccgct ggtggtcgcc
     1321 tgcaccgagg ccagccccag gcaatccata ctaatccctt caaacgccgc attttgccag
     1381 gcatcctgaa tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca
     1441 tggtccgctt tcgttgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga
     1501 aagagcgtgc gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata
     1561 tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg
     1621 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa
     1681 ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac
     1741 tcgccccagg tatcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata
     1801 tctcctggca agacaaagcg ccctggctga ataaagtgca gcccctgctg tttacagtgg
     1861 tggagataat cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggtgct
     1921 agcgggtcca gcccttcgca catcattcgc catttgaccg accattcgcc gcgctgacca
     1981 ttgagtaagc ccgtcatctg gcgtgaccag cttaaatagt cctgcgccag catcggcagg
     2041 tcgagcaacc attcgccggg ataatccaca atctccagat acagcgtgga ggtgtctttg
     2101 aagtggcgta gcagcgaatc gttcgattta aaacgtagcg ccaggcggat ttcactgacg
     2161 ccgcgcgtag gcgtcggcca ggcgggagga tcgccatata actgcgccag tccttcatca
     2221 taggtgaagc gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcaggcgc
     2281 tcttcacgca ccgcgcttaa cagcggcaag cgtgctccgg cgtgaatatt gagcaactga
     2341 ttgaccatcg cagtgataaa cgctgttttg ccgctgcggc ttagtccggt tacggcgagg
     2401 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
     2461 attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca ttcgtaagga tatgaatgtg
     2521 ggggccaggc cattgattat caactgcccg tgttgtcatc gacgggcgaa ctccataaga
     2581 tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt
     2641 gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaactaat
     2701 atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcagcca
     2761 aacaagctag cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc
     2821 cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca tgccatcacc
     2881 gccaaagctg aagaagaagg tttcgacgtg atattgcaga cgtcgcacaa cccggcagaa
     2941 gacttacaaa aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta
     3001 agttcgccag cggatgagtc attttttgcc caactcgata aatacgatat tccggtggtg
     3061 gtgattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt
     3121 ggcgacagca ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa tattgcctgc
     3181 ctgcatgctc cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagc
     3241 ctggctgcgc ataatattgc agtgcgtgat gaattgattg ttgacggtgg ttatacccat
     3301 gaaacagcac tgcaagccgc gagagaatta ttaagccagt caccgttgcc tgaggcggtg
     3361 tttgccactg acagtctgaa attaatgagt atttatcgtg cggcagcaga gaaaaatatt
     3421 gctattccgc agcagttagc ggtggtgggt tatagtaatg aaacgctgtc gtttatatta
     3481 acgcctgcac cgggcggcat cgatgttccg acgcaggagt taggtcaaca aagctgcgag
     3541 ttattattcc gcttaatttc tggaaaaccg tcaccacaga atattaccgt tgccacgcat
     3601 atgacgctga aataaatacc agctaccttg cctgaatgcc ggatgcgatg ctagcgcatc
     3661 ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc
     3721 cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc actgtctcct
     3781 tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc gttctggaaa
     3841 tcataaccgt aaaataaacc tacacggtta aaatcatggc cttcacgttc aatatcatcc
     3901 tgccagtccc agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg
     3961 ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttattg
     4021 cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt
     4081 tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggta
     4141 gtgttcagat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg taaatcgtca
     4201 gtcagtttca catcccagtc tggcgcgatt ttccagcgct gcatattcgc cgtgtcttta
     4261 cccggttcat caacgtagtg ataaccataa ttacggaaac cgccggtcag gccgaaactg
     4321 aaatcatcgt tttcgaggaa ctgataatgc acctccagct ccgggcgatc aaaccacgtt
     4381 ccacgtttac ccgcgctata atctaccggc ccttcctgat aataggccag agcaattctc
     4441 cacggcccgt tggcggcatt aaaatagact gaaggctccg cc
//
LOCUS       DAETQN010000220         4471 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.223, whole genome shotgun sequence.
ACCESSION   DAETQN010000220 DAETQN010000000
VERSION     DAETQN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.223"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..268)
                     /locus_tag="K0W68_004267"
     CDS             complement(<1..268)
                     /locus_tag="K0W68_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075643.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPM"
     gene            497..1105
                     /locus_tag="K0W68_004268"
     CDS             497..1105
                     /locus_tag="K0W68_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBI3075644.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1203..2594
                     /gene="selA"
                     /locus_tag="K0W68_004269"
     CDS             1203..2594
                     /gene="selA"
                     /locus_tag="K0W68_004269"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBI3075645.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2591..4435
                     /gene="selB"
                     /locus_tag="K0W68_004270"
     CDS             2591..4435
                     /gene="selB"
                     /locus_tag="K0W68_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBI3075646.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         1001 a         1139 c         1322 g         1009 t
ORIGIN      
        1 ccatcggcgc gggcgttttt gtccggtaac tgctgtaggt gcgggagagg atgaacggca
       61 gcggggcggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccg agcaggggat
      121 tgaccggatg gccggacgtc actccgccgg ggcacaccga acaggccaca ccggtggggg
      181 caccgatgcg caccccggct gaaccctgaa caatgctacc gccatactgc gtcatgtcgc
      241 cctgacgcgc tgccggtttt ccgctcatct gctcatcctt gttttatcgg gttatttgtc
      301 tgcttccagg caaaacccta cagatttata agaaagcctt aagtgagata tccatcagat
      361 agaaacaatt aggaaaatat ttatatcacc atgataaata tgtgatttat ttggaagtaa
      421 caaaccatca acattttctg ccctttttcc catcaccagg ccatactgat atttttcctc
      481 gcacaaagga ctcggcatga aactcgtcgg tagctacacc agcccgtttg tacgcaaact
      541 ttctattctg ttgttagaaa agggcataac tttcgaattt attaatgaac tgccctataa
      601 cgcggacaac ggcgtggcgc aatttaaccc gttaggaaaa gtgccggtgc tggtgaccga
      661 agagggcgaa tgctggtttg attcgccgat catcgctgaa tatattgagt taatgaatgt
      721 cgctccggcg atgttgccgc gcgatccgct ggagtcgttg cgggtgcgca aaattgaggc
      781 gctggcggat ggcattatgg atgccgggct ggtatcggtg cgtgaacagg cgcgtccggc
      841 ggcgcagcag tctgaagatg aattgttacg ccagcgggag aaaatcaacc gcagtctgga
      901 tgtgctggaa ggatatctgg tcgatggcac actcaaaacc gatacggtca atctggcgac
      961 tatcgccatt gcctgtgctg tcggatatct caatttccgc cgcgttgcgc caggttggtg
     1021 tgtcgaccgc ccacacttag tcaaactggt cgaaaacctg tttagccgcg aaagttttgc
     1081 tcgcaccgaa ccgccaaagg cttgatgcgc gatatgtcct cctgacccat ctcacgttac
     1141 aatccgtggt tatgttaaac gcccttctcc gtgtgagagg gccttgatca gccaggtttc
     1201 ctatgacaac cgaaacgcgt tccctctata gtcaacttcc ggctattgat cgcttattgc
     1261 gcgatagctc cttcctttct ttgcgtgata cttatggtca cacccgcgtg gtggaattgt
     1321 tgcgtcagat gctcgacgaa gcgcgagaag tgattcgtga cagccagacg cttcctgcgt
     1381 ggtgtgaaaa ctgggcgcaa gaagtcgatg cccggttgac gaaagaggcg cagagcgcgc
     1441 tgcgtccggt gatcaacctg acgggaaccg tgctgcatac caaccttgga cgagctttac
     1501 aggcggaagc cgcggtggaa gccgttgcgc aggctatgcg ttcgccagtg accctcgagt
     1561 acgatctgga cgacgccgga cgcggacatc gcgatcgggc gctggcgcaa ctgctgtgcc
     1621 gtattacggg ggcggaagat gcctgtatcg tcaataacaa tgcggcggcg gtgttattga
     1681 tgttggcggc cactgccagc gggaaagagg tggtagtatc tcgcggcgaa ttggtggaga
     1741 ttggcggcgc gtttcgtatt cccgatgtta tgcgtcaggc aggctgcacc ctacacgaag
     1801 tagggaccac caaccgcacg cacgcgaatg attatcgtca ggcggtgaat gaaaataccg
     1861 cactgttgat gaaagtacat accagtaact acagcattca ggggttcacc aaagcgatag
     1921 atgaagcgga actggtggcg ctcggcaaag agctggatgt tcccgtagtg actgatttag
     1981 gcagtggctc gctggtcgat cttagccagt acggtttgcc gaaagagcca atgccgcagg
     2041 agttgattgc ggcgggcgtc agtctggtga gcttctccgg cgacaagttg ttaggcgggc
     2101 cgcaggcagg gattatcgtt ggtaaaaaag agatgatcgc ccgcctgcaa agccacccgc
     2161 tgaagcgtgc attacgcgcg gataaaatga ccctcgcggc gctggaagcc acgttgcgtc
     2221 tttatttaca ccctgaagct ctgagtgaaa aattaccgac cctgcgcctg cttacccgca
     2281 gcgcagaggt cattcaaatc caggcacaac gtttacaggc tccccttgcc gcacattacg
     2341 gcgcggagtt tgcggtacag gttatgccat gtctttcgca gattggcagt ggttcgctgc
     2401 cggttgatcg cctgccgagc gcggcattaa cgtttacacc ccatgatgga cgcggtagcc
     2461 accttgagtc attagccgcc cgctggcgtg aattgccagt gccgatgatt ggtcgtattt
     2521 atgacggacg attgtggctg gatttacgct gccttgaaga tgagcaacgg tttttggaga
     2581 tgttgttgaa atgattattg cgactgccgg acacgttgac cacggcaaaa caaccttatt
     2641 gcaggtgatt actggcgtaa atgctgaccg tctgccggaa gaaaaaaagc gcggcatgac
     2701 catcgatctc ggctatgcct actggccgca gccggatggt cgcgtgccta gttttatcga
     2761 cgtacccggt catgaaaagt ttctttccaa catgctggcg ggcgttggtg gtatcgatca
     2821 cgcgctgttg gtggtggcgt gcgatgacgg cgtgatggcg cagacccgtg agcatctggc
     2881 gattttgcag ctgaccggta acccgatgct gacagtggcg ctgaccaaag ccgatcgcgt
     2941 ggacgaagcg cgtgttgatg aggttgaacg ccaggtaaag gaggtgctgc gggaatacgg
     3001 ttttgctgag gcaaaactgt ttatcaccgc agcaaccgaa ggtcggggaa ttgatgccct
     3061 gcgcgagcat ctgcttcagt taccaggacg tgaacacgcc agccaacata gtgtccgcct
     3121 cgcgatcgac cgcgcattta ccgtaaaagg tgccggtctg gtcgtcaccg gtacggcgtt
     3181 aagcggggaa gtgaaggtag gcgattcact ctggctgact ggtgtaaata aaccgatgcg
     3241 tgtacgtgcg ctgcatgcac aaaaccagcc aacagaaacc gcccatgccg ggcagcgtat
     3301 cgcgcttaac atcgcgggtg atgcggaaaa agagcagatt aaccgtggcg actggctgct
     3361 tgccgatgcg ccgccagagc cgttcacacg ggtgattgtc gagcttcaaa cccatacgcc
     3421 gctgacccag tggcagccgc tgcatattca ccacgccgcc agccacgtta cgggacgcgt
     3481 ttcgctgctg gaagataacc ttgccgagct ggtgttcgac acgccgctgt ggctggcgga
     3541 taacgatcgc ttagtgctgc gcgatatctc ggcccgcaac acgctggcgg gggcgcgtgt
     3601 ggtgatgctt aacccgccgc gtcgcggtaa acgtaagccg gaatatctgc aatggctggc
     3661 gtctcttgca cgggcgcaga gcgatgccga tgcgttatct gttcatctgg aacgcggcgc
     3721 ggttaacctt gcggatttcg cctgggcgcg ccagctcaac ggcgaaggga tgcgcgaatt
     3781 gctgcaacag cctggctata ttcaggctgg ttatagcttg ttgaatgcgc cggttgccgc
     3841 ccgctggcag cggaaaattc tcgacacatt agcgacttat catgagcaac atcgcgatga
     3901 acctggtcct gggcgcgaac gtctgcgacg tatggcgttg ccaatggaag atgaagcgct
     3961 ggtactgttg ctgattgaaa agatgcgcga aagcggcgac atcctcagtc atcacggttg
     4021 gctgcatctg ccggatcaca aagcgggctt cagcgaagag cagcaggcca tctggcaaaa
     4081 agcagagccg ctgtttggtg acgagccgtg gtgggtgcgt gatctggcaa aagagacggg
     4141 taccgacgag caggcaatgc gcctgacgct acgccaggcg gcgcagcaag gaataattac
     4201 cgcgatcgtc aaagatcgtt attaccgtaa cgatcggatt gtcgaatttg ccaatatgat
     4261 ccgcgatctc gatcaggagt gtggttcaac ctgcgcggcg gatttccgcg atcgcttagg
     4321 cgtaggccga aagctggcaa ttcagattct ggaatatttt gaccgcattg gctttacgcg
     4381 tcgtcgtgga aatgatcatt tattgcgcga cgcattatta tttccggaaa aataaggaaa
     4441 tgattaatta atttttaaaa taaattaata c
//
LOCUS       DAETQN010000221         4410 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.224, whole genome shotgun sequence.
ACCESSION   DAETQN010000221 DAETQN010000000
VERSION     DAETQN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.224"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(21..1958)
                     /locus_tag="K0W68_004271"
     CDS             complement(21..1958)
                     /locus_tag="K0W68_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBI3075647.1"
                     /translation="MNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSRSGI
                     LALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPPFYD
                     TLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGDLLI
                     HEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKSNHH
                     GALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFRLAI
                     IQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNENDP
                     GIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYPLFA
                     ALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDYPTS
                     VLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVPATI
                     LAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAEVLP
                     SVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAHSAD
                     MTFRVKPESLILTIFFLGLIP"
     gene            2369..3076
                     /locus_tag="K0W68_004272"
     CDS             2369..3076
                     /locus_tag="K0W68_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBI3075648.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3182..3257
                     /locus_tag="K0W68_004273"
     tRNA            3182..3257
                     /locus_tag="K0W68_004273"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3215..3217,aa:Phe,seq:gaa)
     gene            3455..>4410
                     /locus_tag="K0W68_004274"
     CDS             3455..>4410
                     /locus_tag="K0W68_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBI3075649.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1149 a         1162 c         1058 g         1041 t
ORIGIN      
        1 gatttactgc tacgtcgccc tcagggaatt aaacctaaaa agaaaatcgt caaaattaga
       61 gattccggtt tgacacggaa agtcatatca gcgctatgcg catcctgggg gttcatcacc
      121 actgacggga aactctgctg gcggaacatc gctttttgta ggtctttgac gtcgaaactg
      181 acatacagat cgtgcatctc ctgacacaac tggcgcaggg tgtagtcgcg atagcgcacc
      241 ggatatttgt tataaacgct cggcaacacc tcagccagcg gcgagtcatc ctcaatatgc
      301 tgttcaaatt gcgccagcat cgccaccagt tgtgccagct tctcgtggct ttccgccgga
      361 gttaataaga acagaatgga gttgagatcg cacttctccg gcacaatgcc gttctcacgc
      421 agatagtgcg ccagaatcgt cgccggaacg ccaaagtcgc tatattcgcc ggtttcggca
      481 ttgatacctg gtgtagtgag taacagcttg cacggatcaa caaaatactg atccgcggca
      541 tatccttcaa agccgtgcca cttcgccccc ggctcaaaac tgaaaaaacg gcggtcgctg
      601 gctaacacag atgtcggata atcctgccac aatttgccat caacaacggg cgggataaac
      661 gggcggaaca gcttacagcg cgcaagaata gccttgcgcg actcaatacc caactcaacg
      721 cactcagccc acagccgacg cccactctcc ccttcatgaa ttttggcgtt aacatccagt
      781 gcagcaaaca gcggatagaa agggctggta gaagcatgga gcataaaggc gttattcaac
      841 cgcttatgcg ggcaaaaacg cgcctgtccg cggatatggt tatctttttt atggatctgc
      901 gacgtctgtg agaatcccgc ctgctgtttg tgcaccgact gagtcacaaa gatccccgga
      961 tcgttttcgt taagttctaa tagcagcggc gagctatccg ccatcatcgg gataaactgt
     1021 tcgtaaccga cccacgcgga atcaaacaga atgtaatcac acagatgccc aacggtatcg
     1081 atcacctgac gggcgttata gacagttccg tcataggttc ccagctgaat aatcgccagg
     1141 cgaaacgggc gcggcagctc ggctttttct ggcgcaacgt cgcgaatttg ctggcgcaga
     1201 tactcttcat taaaacagtg cgcatcaata ccgccaatga aaccaaacgg gttgcgtgaa
     1261 gcttccagat agaccgacgt cgcccccgcc tgaatcagcg cgccgtgatg attcgactta
     1321 tggttgttac ggtcgaagag caccagatcg ccacgcgtta acagcgcatt cgtcaccact
     1381 ttattcgctg ccgatgtgcc gttcagcaca aaataggttt tatcggcatg aaagactttg
     1441 gctgcgaatt tctgcgcatc cttcgccgat ccttcatgaa taagcagatc gcccaatttt
     1501 acgtcagcgt tacacatatc ggcgcgaaag acgttctcac caaagaaatc gtaaaaatgg
     1561 cgtccggcag gatgcttttt aaaaaacgca ccatgttgat gtccagggca agcaaaggtg
     1621 ctgttgccca tctcaacgta ctgcgtcagc gtgtcataaa acggtggcag caaattctct
     1681 tcatactgac aggctgcgga ttccagctcc agccactgct gctcgttgcc gttgattacc
     1741 gccgtaacgc ccgcaggtaa ttcaacagca tgttcggaat acaaaaacac cggtagatga
     1801 aaaccggtgc gcttaagcaa cgcaagaatg ccactgcgac tatccgcagc ggtaatgacg
     1861 actgccgcga catccgtaaa atcagtatct cccaacgcca ccacgcggcg atgagtagaa
     1921 agtcgggata ccagttcact actggcggca atattcattg atttcataag cgcaaaaccc
     1981 gtttcgggga agtaagaata ccggacaaag tgtaaaaccc tgccccatga gatatgggtc
     2041 aaactggtca ccagctccga cgaccgccag acatcagtaa aagcagaaac gctctgattt
     2101 tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca tgagcagtaa catagaaagg
     2161 gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta aggagatggc gtgcatcggg
     2221 caaactccgt acagagagga gaaaattcgc gcaatcatgg cacctttcgc taaggcgtgc
     2281 aagccaggac tttgcgacca gacaagccat caacaaggca aaaagttcat aataagaaaa
     2341 tcaaacaata cagctaacag gaactcttgt ggtcatcggc ccttttatca acgctagtgc
     2401 agtcttactg ggtggcgttc tcggcgcact gctcagccaa cgcttaccgg aacgtatccg
     2461 cgtctccatg acatctattt ttggtctggc atcgctgggg attggtattt tactggttgt
     2521 gaaatgcgcc aaccttccag cgatggtttt agctacgcta cttggcgctc taattggcga
     2581 aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc aaagcacaaa atctgtttcg
     2641 tcactcacgt aagaagccag cgcatgaatc ttttattcag aattatgtcg cgattattgt
     2701 cctgttttgc gccagcggca ccgggatctt cggggcgatg aacgaaggga tgaccggcga
     2761 tccgagtatt ttaatcgcca agtcatttct tgatttcttt acggcgatga tcttcgcctg
     2821 ctcgctgggt attgcggtgt cggtaattag tatcccatta ctgatcatcc agctaacgct
     2881 agcgtgggct gccgcgctga tattaccgct gaccacaccg tcgatgatgg cagacttcag
     2941 cgccgtaggc ggtttattgc tgctggcaac cggattacgc atctgtggca ttaaaatgtt
     3001 cccggtggtc aacatgcttc cagcactcct tctggcaatg ccgctttccg ccgcctggac
     3061 cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt gatgatagat tgtgcagtct
     3121 gcagtaaatt gaagaaattt gattgacgag acgaggcgaa tcaggtttaa tgcgccccgt
     3181 tgcccggata gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc ttggttcgat
     3241 tccgagtccg ggcaccaaat tcatatcaac ggacctccac ggaggtccgt ttttcgtttc
     3301 agaacgccac gatttaagcg ttctgcctac aaatcaattc taccgaactc aaccagattc
     3361 cccccacatc aaccccattg tgtgggtata attgcgggca taccccagtt cgacagaatt
     3421 tgtaccctct ttaacgcatt cagaaggctg aactatggca ctgactgacg caaaaatccg
     3481 ggctgcaaag cccactgaca aggcttataa actcactgac ggggctggca tgttcctgct
     3541 ggtacatcct aatggctccc gttactggcg tctccgttat cgtattctgg gtaaggagaa
     3601 gactctggca cttggtgtgt atccagaagt ttctctctcc gaagctcgta caaaacggga
     3661 tgaggcccga aaactgattt cggagggggt tgacccttgc gaacagaaaa gagctaaaaa
     3721 agtagtccct gatttacagc tctcttttga acatattgcg cgacgctggc atgccagtaa
     3781 taaacaatgg gcacaatcac acagcaataa agtactcaaa agccttgaga ctcacgtttt
     3841 cccctttatc ggcaaccggg atatcacaac actcaatacc ccggatctgc ttatccctgt
     3901 tcgtgctgca gaagctaaac aaatttatga aatcgccagt cgtctgcagc aaagaatatc
     3961 tgccgtaatg cgttatgccg tacagtctgg catcatcaga tataatcctg ctctggatat
     4021 ggctggcgca ttgactacgg taaaacgcca gcatcgcccc gctcttgatc tttctcgcct
     4081 gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa cctgtcaccc agcttgccgt
     4141 tatgctgaat ttactggttt ttattcgttc cagtgaactc agatacgccc gctggtctga
     4201 aattgatatt gacaatgcca tgtggactat tccagccgaa cgcgaacccc tgccaggcgt
     4261 aaaattctca caccggggct ccaagatgcg aacaccacat cttgtgccac tcagcaaaca
     4321 ggctgtagcc atactgacag aactacagac atgggctggt gaaaatggtc tgatctttac
     4381 gggagcacat gacccgcgta aaccaatcag
//
LOCUS       DAETQN010000222         4409 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.225, whole genome shotgun sequence.
ACCESSION   DAETQN010000222 DAETQN010000000
VERSION     DAETQN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.225"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(173..2122)
                     /locus_tag="K0W68_004275"
     CDS             complement(173..2122)
                     /locus_tag="K0W68_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBI3075650.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="K0W68_004276"
     CDS             complement(2207..2542)
                     /locus_tag="K0W68_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HBI3075651.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="K0W68_004277"
     CDS             complement(2542..2898)
                     /locus_tag="K0W68_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBI3075652.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="K0W68_004278"
     CDS             complement(2898..4394)
                     /locus_tag="K0W68_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBI3075653.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1020 a         1314 c          970 g         1105 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agccatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagcctta
//
LOCUS       DAETQN010000223         4407 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.226, whole genome shotgun sequence.
ACCESSION   DAETQN010000223 DAETQN010000000
VERSION     DAETQN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.226"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..590)
                     /locus_tag="K0W68_004279"
     CDS             complement(<1..590)
                     /locus_tag="K0W68_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBI3075654.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="K0W68_004280"
     CDS             complement(583..993)
                     /locus_tag="K0W68_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HBI3075655.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="K0W68_004281"
     CDS             complement(999..1532)
                     /locus_tag="K0W68_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HBI3075656.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2598)
                     /locus_tag="K0W68_004282"
     CDS             complement(1510..2598)
                     /locus_tag="K0W68_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HBI3075657.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLVLGENILSCDTERSIRERFSEYQVSGQRPG
                     NDDDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRE
                     TTYTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGP
                     ADAYLPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2595..3986)
                     /locus_tag="K0W68_004283"
     CDS             complement(2595..3986)
                     /locus_tag="K0W68_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HBI3075658.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRKSLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4033..4239)
                     /locus_tag="K0W68_004284"
     CDS             complement(4033..4239)
                     /locus_tag="K0W68_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075659.1"
                     /translation="MTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAG
                     VNVNGESGVTYENCKLNMKDINDD"
BASE COUNT         1056 a         1286 c         1041 g         1024 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcac
     2041 cagcaccagc gccgtcgccg ctttacctgt tccgggtttt cccagaacca gtcgcccgca
     2101 ttcatcgtca taagccagcg tctgaacctg ccccagtaac cggttaagac aatcggcaac
     2161 ggtttcgccg tgttccggct gggcatcaat caccgctgtc tgcggcacgc cagcatcaac
     2221 aacggtgatg ccaaatggcg cagccagttc actgactatt ctgagcaggt tttttccgct
     2281 ctgctggagt ggcaaagcag agcagtcaac cagatcggct gttttgcttc gcccgacaat
     2341 tcccatgctg acgctgctgg cgtcataacg aagcggtagt gcctccacat atccggtgag
     2401 cacaggctca tccccgataa gcacttcaac cagctcacca ttttttatcc ggggttgata
     2461 atcccggctt ccgggccagc gggtggtaat ggcaacatta aaatcccggg caatacggtt
     2521 aatgcccgca ctgatacgga cggatgtcca gccgccccat tcgcgaccgg aaacccgaag
     2581 taaaacggta tcattcatct gacgggtacc cttaatgccc tgaccggaac aaagcccgga
     2641 tgagagatgg catttcgatc caggatatca gtttcacggg acgcatcgtc gtaccatgac
     2701 gcagccagaa caagcgcagg cagaacttcc gctggcgttc gctctgcggt ttcctccgtc
     2761 tgaaccagac gcgcctgaat atcccggttc agttctgtac gtaatgacgt cagctgaaaa
     2821 aacagcctgt catccgccgt acgcctgagc tcctgttcaa tcgccgcgtt cagcgatttg
     2881 cgtatgatgg tgagattttc ccgcgtgggt ggttgcgctc tctcatcctg tcctgtgctg
     2941 gctgccacgc tgtcaagtgc cggatgagaa acgtgaataa tatcggactg acgttcagtg
     3001 gaaccaccaa cagccacaac cgcctgctga tttttcacca ggcttccggg ttgtggcagt
     3061 gaagtgaccg cccttgtggc ttcgctgatt gccgtcgtcc ggatgacagc agccacaagg
     3121 tttgtctgct gtttttgcct gataaccgat gcggaatctg tgggccacac cgcacgcggt
     3181 gccagtcctg ggtccagcgt aatgccggac attgtcgtta tggactgcac cagatcctgt
     3241 gtgttatcca ccagccgggt tccggcccgc caggtatcct gcaacgtatg cacaaaatca
     3301 ctggcaaccg acggcggcat cagaatgacg gataaatcgc cctgcagtag tctcgctccg
     3361 gcagaaacag ccgaattaac cattttgaaa gctgtctgaa cggttcccag catatcggtc
     3421 gtccgggcaa taacatcgtt ctgaataaag tccggtattc ccgcgagatc aaaatcgccg
     3481 aacatgtctt caatcaactc atccaggaat acggacgact cctccagttt tctggcggtt
     3541 gcagctccgg cgaccggaaa tgacagttcc ccgctctcaa caaactgaaa tgaaacccgg
     3601 cacatacggc cttcagtgcc ggagtgagaa acggtcacct gcccgtcaat acagccctgc
     3661 atttcaccga actgcggatg gatcagcgtc cccggcccgg cggtttcaat cgctgtaatc
     3721 agcctgtcgc gctgctctgc gtaatcatca ccaacgagat acgcattaat cgtcagccgt
     3781 cgcgtggcac gcccgagatc ttccgtgtac ggtttgtcac ggtttgggta ttcatggacc
     3841 tgaacgcggc gtccgaacga accctcgtcg ctctccaccg aaaacggaac gccacgaaat
     3901 gaagcgtcgt aaagattatc gcgccaattg atacctgaag atgcagaaaa gagtgaagaa
     3961 agagaaggta aagaaggtaa gtccatactg tctccactat aaaaaaaccg ccaatttctg
     4021 gcggcttata tattaatcat cattaatatc tttcatattc aatttgcaat tttcgtaggt
     4081 cactcccgac tcgccattaa cattaactcc agctctgata ttcccgtttt cttttacatc
     4141 tacaaaaaac tggcctcttg gcatcttgaa cgcaaatgta aaaccaacag tatctgaatc
     4201 aaaatcatca tctttatata ttgggatcaa aaatgtcatc gtcgctggtg atgcattgtt
     4261 tgcaaaatca aatacaccta tacttttgcc gaattcataa gaagcttttc tcaatataat
     4321 cgcaccgctg gcagaagggg atgggcacga tatcctcagt tgtctgaatc catctgtatc
     4381 caagtcaacg tcattatctg cagatgt
//
LOCUS       DAETQN010000224         4404 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.227, whole genome shotgun sequence.
ACCESSION   DAETQN010000224 DAETQN010000000
VERSION     DAETQN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.227"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(139..3654)
                     /locus_tag="K0W68_004285"
     CDS             complement(139..3654)
                     /locus_tag="K0W68_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI3075660.1"
                     /translation="MDKNALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..>4404)
                     /locus_tag="K0W68_004286"
     CDS             complement(4106..>4404)
                     /locus_tag="K0W68_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBI3075661.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1141 a         1127 c          905 g         1231 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggt aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt acag
//
LOCUS       DAETQN010000225         4398 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.228, whole genome shotgun sequence.
ACCESSION   DAETQN010000225 DAETQN010000000
VERSION     DAETQN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.228"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..698
                     /locus_tag="K0W68_004287"
                     /pseudo
     CDS             1..698
                     /locus_tag="K0W68_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            705..1451
                     /locus_tag="K0W68_004288"
     CDS             705..1451
                     /locus_tag="K0W68_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI3075662.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1466..1888
                     /locus_tag="K0W68_004289"
     CDS             1466..1888
                     /locus_tag="K0W68_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBI3075663.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1946..2302
                     /locus_tag="K0W68_004290"
     CDS             1946..2302
                     /locus_tag="K0W68_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075664.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2471..3136
                     /locus_tag="K0W68_004291"
     CDS             2471..3136
                     /locus_tag="K0W68_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBI3075665.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            3364..3519
                     /gene="hokD"
                     /locus_tag="K0W68_004292"
     CDS             3364..3519
                     /gene="hokD"
                     /locus_tag="K0W68_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBI3075666.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            3769..4056
                     /locus_tag="K0W68_004293"
     CDS             3769..4056
                     /locus_tag="K0W68_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075667.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            4095..>4398
                     /locus_tag="K0W68_004294"
     CDS             4095..>4398
                     /locus_tag="K0W68_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBI3075668.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAE"
BASE COUNT         1193 a          972 c         1212 g         1021 t
ORIGIN      
        1 tattcgcttc tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc
       61 tcgcggcgtt acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc
      121 gttaattcag gttggatggc tggtcgaaaa tgacggaggg ctttctctac ctaactttga
      181 acgtcataac ggaaaaagct ctaaaaaaaa cgggcggtta caaacgagcg agtaacaaaa
      241 atacgcgaac tgaaacgaaa aggtaacgct gccagcgtta cacaaacgga tcaaaaagcg
      301 ttaccagagg aagaggaaga ggaagaggaa gaggaagagg aagaggaaga tctaaatact
      361 gatctccccc tcgccaaaaa cgagcgtcta aaaaattcga gccggaggct attgagctgc
      421 ccgattggtt gccggaaaca ctctggcatg agtgggtccg gttcagacag gcattgcgaa
      481 aaccgattcg aacggagcag ggcgctaacg gggcgatacg ggaactggaa aaattccgtc
      541 agcagggttt tacacctgag caggtgattc gacacagcat cgccaatgaa taccagggcc
      601 tgttcgcgcc gaaaggtgtt cggcctgaga cgttgctccg acaggttaac accgtctcgt
      661 tgccggacag tgcgatcccg ccaggcttca gggggtaacg gaccatgaaa aatattgcga
      721 caggcggcgt tctggaacgc atccgccgac tgaccccgcc acatgtaacc gccccattca
      781 gaacggtagc ggagtggcgc gagtggcaac ttgctgaagg ccagaaacgt agcgaggaga
      841 tcaaccgcct gaatcgccag ttgcgggtgg aaaaaattct gaatcgctca ggcatccagc
      901 cgttgcaccg taaatgctcg tttgcgaatt accaggtgca gaacgacggc cagcgatacg
      961 cgttaagcca ggcgaaatcc atcgccgatg aactgatgac cgggtgtaca aattttgcgt
     1021 tcagcggaaa acctggtacc gggaagaatc acttagcggc agctatcggg aatcgcctgc
     1081 tgaaagacgg tcagacagtg attgtggtta ccgtggctga tgttatgagc gccctgcacg
     1141 ccagctatga cgacgggcag tcaggcgaaa aatttttgcg ggagctgtgc gaagtggatc
     1201 tgctggttct tgatgaaatt ggcattcagc gcgagacgaa aaacgagcag gtggtgctgc
     1261 accagattgt tgatcgccgg acagcgtcga tgcgcagcgt ggggatgctg acaaacctga
     1321 actatgaggt gatgaaaaca ttgctcggcg agcgggtgat ggatcgcatg gtcatgaacg
     1381 gcgggcgctg ggtgaatttt aactgggaga gctggcgtcc gaatgttagc cattcgaggg
     1441 ttgttaagta gtttcaggag gatttatggc gaaacctttt actcccgaac agcgggaaga
     1501 actgaagacg cgaattgtgg aactcgtgca tcaggacggt cgggtcacga ttcggcagtt
     1561 gtcagatgaa acaggtatca gtcgtgcgtc tgtcggtcgc ttatgcatag aactggtcgc
     1621 aagtggtgat gtatataatt ctggctacgg cttattcccg tctgaacagg ctcgcaagga
     1681 ctggcaaagc gcccgcaaaa aactctcgag agtaaaggtg aggaaaccgg ttgttgttga
     1741 tccggacctt atctggtcat tacctgacgg agaaatacgc cgctacgaca ggcgcctgaa
     1801 tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg ctttctatca
     1861 gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc aattaccgct
     1921 ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt taagcaatat
     1981 cgttttattt ccggtaaaag aggatgaccc tcgtaatcag gttaattttc tttatgagcc
     2041 atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag agcgtcaggt
     2101 ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct ctgtggcaaa
     2161 aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac aggaaaggcg
     2221 gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga tacgcagggc
     2281 gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat ccttaaccgg
     2341 agggatttct gcaccctcaa atcatcagga gaccacccga aagggcgggg agcagtcaca
     2401 catctgtttc cgatagcccc gttctaatgc tacactcttt gatattttta tgaccccaat
     2461 aaacatattt atgacagttg ctgatttcaa acggcccaaa ttggagctcc caaacggggc
     2521 aaacaaacta ctactgcact cttgctgtgc tccatgttcc ggtgaggtga tggaggcgct
     2581 tcaggcctcg ggaatcgact acaccatctt tttctacaac ccgaacattc atcctcagaa
     2641 agagtattta attcgtaagg atgagaatat tcgctttgct gaacaacacg gcgtgccgtt
     2701 tattgatgct gattacgaca cagacaactg gtttgaacga gccaaaggaa tggaatggga
     2761 gcccgaacga gggatccgtt gcaccatgtg ttttgacatg cgttttgagc ggacagcgct
     2821 gtacgccgct gaaaatggtt tcagtgtgat cagcagttca ctgggcattt cacgctggaa
     2881 aaatatgcag caggttaacg actgtgggcg gcgagccgtc gcgcattatc cgggcatggt
     2941 gtactgggat tataactggc gcaagcaggg cggctcgtcc cgtatgattg aaatcagcaa
     3001 gcgcgaaaaa ttctatcagc aggaatattg tggctgtgtg tattctctgc gcgataccaa
     3061 tctacaccgc aaatctcagg gacgccctct tatcaaaatt ggtcaactcc actacggtaa
     3121 agaagagaag gagtgatttt atggggcacc tttctgattg atttcatatt ggcgaggtaa
     3181 gtagaatgac tgcgggtgct tgaggctatc tgcttcaggc atgaacacca aaaggcagat
     3241 agagaaaagc cccagttaac attacgcgtc cggcaagacg cttaacatta atctgaggcc
     3301 aatttcatgc tttgcacatg taggttagcc tcttacatgc cgaaaggcaa ggagaagcag
     3361 gctatgaagc agcaaaaggc gatgttaatc gccctgatcg tcatctgttt aaccgtcata
     3421 gtgacggcac tggtaacgag gaaagacctc tgtgaggtac gaatccgaac cggccagacg
     3481 gaggtcgctg tcttcgtaga ctacgaatcc aggaagtaag agtgaccggg cggggagctg
     3541 atcccatccc cgcccacctc tgatgtgtca ggcatcctca acgcacccgc acttaacccg
     3601 cccatcgctg tgatctctca gcgtttcggc gggttttttg ttgtttattt ccggtgaatt
     3661 tgattcgcgc accttcgcag atagaatcga ctcacttaag tagcgcgcag ggagaagagg
     3721 gatggacccc gaacagggga gagctattta tctggaagga ttctgaagat gaaaatcgaa
     3781 gaattgcgtg aaatttttag tgaaaatggc ctctatgctg tgcgcgttga gaatggagcg
     3841 attgtcagcc attgccgcat tagatgtttg caatctcaac aaaggaagag cggtgctgtg
     3901 ttattttatt tttgtaatgg acttctgacg gacggtttta ttttgcgtga ggacgaattt
     3961 gtcacatcat tacgggtttt gaaagagatt ggttttaagg ctggttttct gcttttgctg
     4021 aagaataaac tcatctacaa tcttgagcag gattgaactc ctgctgtgta acaccgtgcc
     4081 accggagaaa gccgatggca catatacaac tggtcaaaca aacctcttcc ggattacttc
     4141 tcccggcgac gccggagagt tgcgattttc tgcatcaaat caaaataggt gagtggatac
     4201 acgcagactt taagcgtgtg tgtaactacg cattccacaa gcgttttttc aaactcctgc
     4261 aactgggttt cgattactgg actccggtcg gtggggcgat cacgcctcgc gaacgaaaac
     4321 tggtgtccgg tttcgttgat tacctgtgtg aatcagtagg ccgggaacat acgccagctc
     4381 tgagcgaagc cgcagagc
//
LOCUS       DAETQN010000226         4361 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.229, whole genome shotgun sequence.
ACCESSION   DAETQN010000226 DAETQN010000000
VERSION     DAETQN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.229"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            115..528
                     /locus_tag="K0W68_004295"
     CDS             115..528
                     /locus_tag="K0W68_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBI3075669.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(573..890)
                     /gene="hspQ"
                     /locus_tag="K0W68_004296"
     CDS             complement(573..890)
                     /gene="hspQ"
                     /locus_tag="K0W68_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBI3075670.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="K0W68_004297"
                     /pseudo
     CDS             complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="K0W68_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            2233..2511
                     /gene="yccX"
                     /locus_tag="K0W68_004298"
     CDS             2233..2511
                     /gene="yccX"
                     /locus_tag="K0W68_004298"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBI3075671.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="K0W68_004299"
     CDS             complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="K0W68_004299"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBI3075672.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="K0W68_004300"
     CDS             complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="K0W68_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBI3075673.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3794..3881)
                     /locus_tag="K0W68_004301"
     tRNA            complement(3794..3881)
                     /locus_tag="K0W68_004301"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3845..3847),aa:Ser,seq:tga)
     gene            4191..>4361
                     /locus_tag="K0W68_004302"
     CDS             4191..>4361
                     /locus_tag="K0W68_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3075674.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFA
                     YTASQPGTHQKII"
BASE COUNT         1149 a         1108 c         1072 g         1032 t
ORIGIN      
        1 atacttacta acatatagac atatttcccg cttttgctct cattcattcg tattagctgc
       61 atggttggca tgtcggcttt gtcgtacact ttgttaaaca gtctggagga aataatgaaa
      121 gaaaccgata ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc
      181 gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac
      241 gtcattccgg tttcgccaaa agttgccggc aaaacgctgc tgggccagca ggggtatggc
      301 acgctggcag acgttccgga aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg
      361 gcgtggggcg tggcacagga agctattgcc attggcgcga aaaccctctg gatgcaactg
      421 ggggtgatta atgagcaagc ggcagtactg gcacgggatg ccgggttgaa tgtggtgatg
      481 gatcgctgcc cggctattga gatccctcgc ctgggcctgg ccaaataaaa aatccccgga
      541 aggcaaaaac cttccgggga ttcgttcagg gatcagttac gcagacgcgg ggcctggagt
      601 tgtttgcgga tggtctgcgc cagctcatcc atggatggct gttcaggatg ttcatcctgc
      661 aattcgctac ttagctgggc ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc
      721 tccatcacta cgtgatacca cggagcggcg cgaagctcgt catttaccgc cagctcatca
      781 ggcgacggtt cagagagcga ataaaccggg tcgatatcca cgaccactcc gagataacct
      841 aacagggaat ggcggacctg ctggccgata ccgaatttgc tggcaatcat agtcacctcc
      901 cgggaaatct ttacctttga tataagggta aaattccaca tttcaagtta catgacgcga
      961 caggcaaacc ctttcagata tagcccttcc ggataggtag cgatcaccgg atgatcggct
     1021 gcctgacgga actgctctat aaattgtaca tcacggccgg catcaattgc ggcatccgcg
     1081 atgattttct gaaataaatc gctggtcatc agaccggaac aggagaaagt caggagaata
     1141 ccgccttcat tcagcaactg aatcgccagc atgttgatat ctttatagcc acgacacgcg
     1201 cccatcaact ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat
     1261 ttttcaccgc gatcgcgata agtacgcagc aatttaaaga catcatcacg gacaaactca
     1321 gccttgctca gatccagttt gttcagctca acgttctgcc gtgcaatatc cagcgcttcc
     1381 taggaggtat caacgctgac aacctggctg caaccgccca tcagtgccga tacggcgaaa
     1441 ccaccggtat aggagaaaca gttcagcaca cgtttatttt caacgtagcg gcgggtagcc
     1501 aggcggctgt ctcgctggtc caggtagtag cccgttttgt gtccgtgctg aatatccacc
     1561 agcagcttca tgccgtgttc ttcaatcggc agcagggcag gcggcaactc gccggtgacg
     1621 aggccctggg tcagctccat cccttctttt ttacgtaccg caacatcgct gcgatcgtaa
     1681 atcgcacatt ccgggtacag cgtttgcagg gcactaatta atgccgcgcg ctgatattct
     1741 gccccagcac tcagcagttg cagcaccaga aaattaccga aacgatcgat agtaataccc
     1801 ggcaggccat cagattctcc ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc
     1861 agtcagtcac gccatttttg tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata
     1921 gactcagacg ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg cgaataagcg
     1981 ccgcgtgcta accattttcc ctgatgatca acaatatcga tggtttcacc gaggctggct
     2041 ttaccttcca tgcgggcaac ggccccggaa aagacccacg gatggcgacg aagtaatgat
     2101 ttttcgcgcc ctttggctaa cactaaacgt acactcataa tttacttttc tgtcgatgca
     2161 aagaaatggg cgtcattgtc cggagttttc catggatttg caacgcgtcc attaaggata
     2221 aggagagcag caatgtcgaa agtctgcata attgcctggg tttacgggcg agttcagggc
     2281 gtaggatttc gctacaccac acagtacgaa gcgaaaagac tgggtttaac cgggtacgcc
     2341 aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg gcaggtggaa
     2401 aaattaatgc agtggttaaa gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc
     2461 agcgagccgc atcatccctc gggggaatta accgattttc gtatccgtta aatacatttt
     2521 accggttttg gcaggccagc aattttggtg gcttgcttag ccggaccttt cggaaacagt
     2581 cggtataaat agcggctgtt acctttttct tcgccaaatt tattcgccat cgcttttacc
     2641 agcatacgaa tcgccggaga agtattgaat tccagataga aatcacgcac aaaacgcacc
     2701 acttcccagt gttccggtga cagcgaaatc ccttcgttct ctgcaatcac cactgccagc
     2761 ggctcactcc actggctgct ttctttgaga tagccttcgg tatccgtttc tatctcttta
     2821 ccttcgaaga tcagcataat tattactacc ttaatcagac tggcggcagt gtaacaaaaa
     2881 acaaagcccc gcataaagcg aggctatgaa agtgttagcg ggtgagatta atcgcggctg
     2941 gcgaagccca gaatgctcag caggctgacg aagatgttgt acagcgaaac atacaggcta
     3001 accgtggcac gaatatagtt cgtttcaccg ccatgaacga tgttgctggt ttcaaacaaa
     3061 atagcgccag aggagatcag aatgaagacc gcgctgatcg ccagatgcag agcaggcagc
     3121 tgcaggaaga tattagcaac cataccaatc agcaccacca caatacccgc catcagcata
     3181 ccgccgagga acgacatatc tttgcgggtg gtcagcacat atgcagagca gcagaagaac
     3241 actaacgccg ttccgcccag tgccatagcg attacgtcac ccattccggc agacagatag
     3301 gtgttcagaa taggtccgag gatataaccc agaaaaccgg taaaggcgaa tgcggagata
     3361 atcccggtcg gcttattcgc cgttttatag gtcaggaaca tcaaaccata catacccacc
     3421 agcgtcagaa tcagacccgg agatggcagc atcagcaccg tactggcagt tgcggtaatc
     3481 gccgaaaagg ccagcgtcag gctcagcaga aaataggtat tacgcagcac cttatgggtg
     3541 ctaagcagtg atgtacggtc atgtgaagaa ctaacaatac gatccattga gtcactctct
     3601 atgacagatg taattaatta agcagcataa tgataatgcg taagggcacc cagaagtttt
     3661 acccatcttt acgcatttga tctggaacag gtttaacagc ggattatcag gtcattaagc
     3721 aaatataacg ccctgagaat ttcgacaggc aaaagaaaaa ggggttagca tttagctaac
     3781 cccttatctt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
     3841 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
     3901 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
     3961 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
     4021 tttactcgtt tagcaaccgg ctaaacatcc ccaccgcccg gcaaaaagaa aaataggtcc
     4081 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa aagttggtga
     4141 tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg
     4201 tttctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca
     4261 ctgccggaca tcagcgctat ctctgctctc actgccgtaa aacatggcaa ctgcagttcg
     4321 cttacaccgc ttctcaaccc ggtacgcacc agaaaatcat t
//
LOCUS       DAETQN010000227         4326 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.230, whole genome shotgun sequence.
ACCESSION   DAETQN010000227 DAETQN010000000
VERSION     DAETQN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.230"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            147..3239
                     /gene="ebgA"
                     /locus_tag="K0W68_004303"
                     /pseudo
     CDS             147..3239
                     /gene="ebgA"
                     /locus_tag="K0W68_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
     gene            3236..3685
                     /locus_tag="K0W68_004304"
     CDS             3236..3685
                     /locus_tag="K0W68_004304"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBI3075675.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKMVLKVTIEDGYFHNK"
     gene            3748..>4326
                     /locus_tag="K0W68_004305"
     CDS             3748..>4326
                     /locus_tag="K0W68_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI3075676.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPN"
BASE COUNT         1043 a         1166 c         1089 g         1028 t
ORIGIN      
        1 acagtgctgc cgtttatttt cgtgatccag ttaaagtaaa tgcatttacc tgtcactttt
       61 tagtaaaaat tttactaaac tccccagcaa ttacacaaac taccatcacc atgaatggtt
      121 ccgatttctc tctaccggga ggccctatga atcgctggga aaacattcag ctcacccacg
      181 aaaaccgact tgcgccacgt gcgtactttt tttcatatga ttctgttgcg caagcgcgta
      241 cctttgcccg cgaaaccagc agcctgtttc tgcccttaag cggtcagtgg aatttccact
      301 tttttgacca cccgctgcaa gtgcctgaag ccttcacctc tgagttaatg gctgactggg
      361 ggcatattac cgtccccgcc atgtggcaaa tggaaggtca cggcaaactg caatataccg
      421 acgaaggttt tccgttcccc atcgatgtgc cgtttgttcc cagcgataac ccaaccggtg
      481 cctatcaacg tattttcacc ctcagcgacg gctggcaggg taaacagacg ctgattaaat
      541 ttgacggcgt cgaaacctat tttgaagtct acgttaacgg tcagtatgtg ggtttcagca
      601 agggcagtcg cctgaccgca gagtttgaca tcagcgcgat ggttaaaacc ggcgacaacc
      661 tgttgtgtgt gcgcgtgatg caatgggcgg actctaccta cgtggaagac caggatatgt
      721 ggtggtcagc ggggatcttc cgcgatgttt atctgatcgg aaaacaacta acacatatta
      781 acgatttcac cgtgcgtacc gactttgacg aagcctattg cgatgccacg ctttcctgcg
      841 aagtggtgct ggaaaatctc gccgcctccc ctgtcgtcac gacgctggaa tataccctgt
      901 ttgatggcga acgcgtggtg cacagcagcg ccattgatca tttggcaatt gaaaaactga
      961 ccagcgccag ctttgctttt actgtcgaac agccgcagca atggtcagca gaatcccctt
     1021 atctttacca tctggtcatg acgctgaaag acgccaacgg caacgttctg gaagtggtac
     1081 cacaacgcgt tggcttccgt gatatcaaag tgcgcgacgg tctgttctgg atcaataacc
     1141 gttatgtgat gctgcacggc gtcaaccgtc acgacaacga tcatcgcaaa ggccgcgccg
     1201 ttggaatgga tcgcgtcgag aaagatctcc agttgatgaa gcagcacaac atcaactccg
     1261 tgcgtaccgc tcactacccg aacgatccgc gtttttacga actgtgtgat atctacggcc
     1321 tgtttgtgat ggcggaaacc gacgtcgaat cgcacggctt tgctaatgtc ggcgatatca
     1381 gccgtattac cgacgatccg cagtgggaaa aagtctacgt cgagcgcatt gttcgccata
     1441 ttcacgcgca gaaaaaccat ccgtcgatca tcatctggtc gctgggcaat gaatccggct
     1501 atggctgtaa catccgcgcg atgtaccacg cagcgaaggc gctggatgac acgcgactgg
     1561 tgcattacga agaagatcgc gatgctgaag tggtcgatat tatttccacc atgtacaccc
     1621 gcgtgccgct gatgaatgag tttggtgaat acccgcatcc gaagccgcgc atcatctgtg
     1681 aatatgctca tgcgatgggg aacggaccgg gcgggctgac ggagtaccag aacgtcttct
     1741 ataagcacga ttgcattcag ggtcattatg tctgggagtg gtgcgaccac gggatccagg
     1801 cacaggacga ccacggcaat gtctggtata aattcggcgg cgactacggc gactatccca
     1861 acaactataa cttctgtctt gatggtttga tctattccga tcagacgccg ggaccgggcc
     1921 tgaaagagta caaacaggtt atcgcgccgg taaaaatcca cgcgcgggat ctgactcgcg
     1981 gcgagttgaa agtcgaaaat aaactgtggt ttaccacgct tgatgactac cccctgcacg
     2041 cagaggtgcg cgccgaaggt gaaacgctcg cgacgcagca gattaaactg cgcgacgttg
     2101 cgccgaacag cgaagccccc ttgcagatca cgctgccgca gctggacgcc cgcgaagcgt
     2161 tcctcaacat tacggtgacc aaagattccc gcacccgcta cagcgaagcc gggcattcta
     2221 tcgccactta tcagttcccg ctgaaggaaa acaccgcgca gccagtgcct ttcgcaccaa
     2281 ataatgcgcg tccgctgacg ctggaagacg atcgtttgag ctgcaccgtt cgcggctaca
     2341 acttcgcgat caccttctca aaaatgagtg gcaaaccgac atcctggcag gtaaatggcg
     2401 agtcgctgct gacccgcgag ccaaagatca acttcttcaa gccgatgatc gacaaccaca
     2461 agcaggagta cgaagggttg tggcagccga atcatttgca gatcatgcag gaacatctgc
     2521 gcgactttgc cgtagaacag agcgagggtg aagtgttgat catcagccgc acggttattg
     2581 ccccgccggt gtttgatttc ggaatgcgct gcacctacat ctggcgcatc gctgccgatg
     2641 gccaggttaa cgtggcgctt tccggcgagc gttacggcga ctatccgcac atcattccgt
     2701 gcatcggttt caccatggga attaacggcg aatacgatca ggtggcgtat tacggtcgtg
     2761 gaccgggcga aaactacgcc gacagccagc aggctaacat catcgatatc tggcgcagca
     2821 ccgtcgatgc catgttcgag aactatccct tcccgcagaa caacggtaac cgtcagcatg
     2881 tccgctggac ggcactgact aaccgccacg gtaacggtct gctggtggtt ccgcagcgcc
     2941 caattaactt cagcgcttgg cactatacct aggaaaacat ccacgctgcc cagcactgta
     3001 acgagctgca gcgcagtgat gacatcaccc tgaacctcga tcaccagctg cttggcctcg
     3061 gctccaactc ctggggcagc gaggtgctgg actcctggcg cgtctggttc cgtgacttca
     3121 gctacggctt tacgttgctg ccggtttctg gcggagaagc taccgcgcaa agcctggcgt
     3181 cgtatgagtt cggcgcaggg ttcttttcca cgaatttgca cagcgagaat aagcaatgag
     3241 gatcatcgat aacttagaac agttccgcca gatttacgcc tctggcaaga agtggcaacg
     3301 ctgcgttgaa gcgattgaaa atatcgacaa cattcagcct ggcgtcgccc actccatcgg
     3361 tgactcattg acttaccgcg tggagacaga ctccgcgacc gatgcgctat ttaccgggca
     3421 tcgacgctat tttgaagtgc attactacct gcaagggcag caaaaaattg aatatgcgcc
     3481 gaaagagaca ttacaggtag tggaatatta tcgtgatgaa actgaccgtg aatatttaaa
     3541 aggctgcgga gaaaccgttg aggtccacga agggcaaatc gttatttgcg atatccatga
     3601 agcgtatcgg tttatctgca ataacgcggt caaaaaaatg gttctcaaag tcaccatcga
     3661 agatggttat ttccataaca aataacaact acggcggcaa aaggagtttg ccgccaccgc
     3721 taccctactc attttcggag atgtgttatg tctgatacca aacgtaatac aatcggcaaa
     3781 ttcggcttgc tctcgctgac ttttgccgcc gtttacagct ttaacaacgt tatcaataat
     3841 aatattgatc ttggactggc ctcggcaccg atgtttttcc tcgcgacgat tttttatttt
     3901 attcccttct gtctgatcat cgcagaattt gtttcgttaa ataaaaactc agaagccggt
     3961 gtctacgcat gggtaaaaag ttcgctgggc ggacgttggg catttattac tgcctatacc
     4021 tactggttcg taaacctgtt ctttttcacc tcactgttgc cgcgcgttat tgcttatgct
     4081 tcgtatgcct tcctcggata cgaatatatt atgacgccgg ttgccaccac cattatcagt
     4141 atggtgctgt tcgccttctc cacctgggtt tccaccaacg gggcgaaaat gttggggcca
     4201 attacctccg tcacttcaac gctgatgctg ctgttaacgc tctcctacat tttactggca
     4261 ggtacggcgc tggttggcgg cgtacagcct gctgacgcca tcaccgttga cgcgatgatc
     4321 ccgaac
//
LOCUS       DAETQN010000228         4290 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.231, whole genome shotgun sequence.
ACCESSION   DAETQN010000228 DAETQN010000000
VERSION     DAETQN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.231"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            225..1067
                     /locus_tag="K0W68_004306"
     CDS             225..1067
                     /locus_tag="K0W68_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBI3075677.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            1070..2158
                     /gene="capU"
                     /locus_tag="K0W68_004307"
     CDS             1070..2158
                     /gene="capU"
                     /locus_tag="K0W68_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBI3075678.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            2163..3113
                     /gene="virK"
                     /locus_tag="K0W68_004308"
     CDS             2163..3113
                     /gene="virK"
                     /locus_tag="K0W68_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HBI3075679.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            3178..4122
                     /gene="lpxM"
                     /locus_tag="K0W68_004309"
                     /gene_synonym="msbB"
     CDS             3178..4122
                     /gene="lpxM"
                     /locus_tag="K0W68_004309"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBI3075680.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         1225 a          799 c          980 g         1286 t
ORIGIN      
        1 ttcaggtagc caaattcagt gtgccacctc aaggttctta gtgagtcgtt tttaaaagat
       61 atgataatat atcattgttt atgaattgtt gatgtttaat ttagtaaatt atatcaaata
      121 tgccatgggt ttgctatgtt attcatccag tatctggaaa taaaatgcac cagctttatt
      181 gggttttcct tttgtctctg cttactaggt gccagaaact ttaaatgtta aacgaagggg
      241 gcattttgtt caaagccaat catgttccgg tattaatgta tcatcatgtc agtcattgcc
      301 ccggactggt tacactttct cccgtgacat tccggaagca aatgaaatgg ttggctgaaa
      361 acaactggaa aacactgtca tcagatgaac tggagttttt ttatcggggc ggaaaactcc
      421 cccggaaaag cgttatgttg acatttgatg atggatacct tgacaactgg tttcaggtat
      481 atcctttatt aaaagagttt aatctcaaag cacatatttt tttaattacc ggttttattg
      541 ggaatggtcc ggttcgtcac tctcctggaa aggaatattc tcatcgcgat tgcgaacatc
      601 aaatagcgac agggaatgct gataatgtta tgcttcgctg gtctgaagta aacgaaatgt
      661 tgcaatctgg tctggtcgaa tttcatgttc atacgcatac tcatacacgc tgggataaaa
      721 agttcagttc acgagaagaa cagtgtaaac atcttcggca ggacttgttg agcgggagag
      781 aatatttgaa agaaatgaca ggaaaatgca gtaaacattt gtgttggccg gaaggttatt
      841 ataacaagga ttatattcag gtcgctgaag aacttggatt ctattattta tatacaacag
      901 aaagaagaat gaatgctcct gctaaaggaa ctacacggat aggaagaata agcaccaaag
      961 aacgggaaag ttgcgcatgg ctgaaaagac gactttttta ttatacaaca ccgttttttt
     1021 catcgttgct ggcctttcat aaggggccaa gattaccgga tgattaataa tgaatatact
     1081 atttacggaa tcatcaccga atataggtgg tcaggaatta caggctgttg ctcaaatgaa
     1141 ggccctgaag aaaatggggc attcagttct gcttgtctgc agggaaaaca gcaaaattgc
     1201 ttttgaagcc agtaaattgg gaattgatat cacattcgcg ttatttcgaa acagtcttca
     1261 catccctact gcatggagat tactcggaat agttcatggt tttcagccca atgcaatcgt
     1321 ttgtcacagt gggcatgata gcaatattgt tggtttagta cggttattca cttggaaaca
     1381 tccattcaga attatcaggc aaaagacata tttgacacga aaaacaaaag ttttttcaat
     1441 aaatcatttt tgcgatgagg tgattgttcc cggaacaagt atgaagacac atctggagca
     1501 ggaaggatgt cgaacccggg ttactgttgt gcctccaggc tttgatttcc agaaattata
     1561 cgttgattcc cgaaacagtt tgcctccaaa tgttctttct tggctggcgt cccgaagggg
     1621 atgccccgtt attgctcagg taggaatgtt gcgcccggaa aaagggcacg aatttatgtt
     1681 gaatttactg ttccatttaa aaatgaatgg acgacagttc tgttggttga ttgtggggtc
     1741 tggttcgcct gaactgcggg agcatttaca gtatcagatt gacagtatgg gcatgcatga
     1801 tgatgttttt attgctgaca atgtttttcc tgccgccccc gtatatcggg ttgccagtct
     1861 ggtggttctg ccttcagaaa acgaatcgtt tggtatggta ctggcagaag catcggcatt
     1921 ttctgtgcct gtactggcca gtcagattgg tggaatccct gatgttattc agaacaacca
     1981 gaccgggaca ttgttaccag caggtaataa gcacgcatgg atgtgcgccc tgaatgattt
     2041 ttttaatgac cctgggcgtt tttatcagat ggctcgccag gcaaaacagg atatagaaga
     2101 gcggtttgat attaataaaa ctgcgttaaa aatactcaca ttagcgaagc acaagtaaca
     2161 atatgttttc tgtaagtaac ttatcattta tcggtttcct taaaaggatt gttttttcct
     2221 cagattcact cccggggaag tgggaacaca gaaaattccg gttcatgtac attttgcgat
     2281 gtgctataaa tccggttgcc agtattcgat attattacga actgcgttcc ttgcagtgca
     2341 ttgaggatat tctggcaata caacccacgt tgccagcaag aattcatcga ccttatttgc
     2401 ataaaggggg gcgagcctgg tcccgggggc aatatattct tgagcattat cgctttgtgc
     2461 aaaatttgcc agaaaaatat tctgaatttt tgtttccaca aaaatcggta tcattagtcc
     2521 aattcatcgg gaaagatgga gaggattttg atatccagtg ttctcccagt ggttttgacc
     2581 gggaaggaga gttgatgttg tcattgtttt tcaataaaat agtgattgcc cggttaacat
     2641 tttcagtcat tctgactcaa aatggacaca ccgcttttat tggcggatta cagggagccc
     2701 ccaaaaatac cggacctgat gttattcggt gcgcaacccg ggcctgttat gggcttttcc
     2761 caaaacggat tatttttgaa gcattttgtg cgttgatgaa agcttgtaat gtctctgagt
     2821 gtctggcagt cagtgagcac agtcatgttt tccggcaatt gagatactgg tatcagaaac
     2881 gcaaaacctt tgttgctgtc tacagtgatt tctgggagtc tgtagcaggg aaaacctgtg
     2941 gtgactggta caaattacca acacaggtgg ttcgaaagcc gctaagcaat atagctagta
     3001 aaaaacgctc tgagtatcga aaacgatacg ctttgttgga ttatatccat gaaactgcta
     3061 tccgctcttt ggatgcatat cctgtaaact cagaacatca ggacttaaat taaggtttga
     3121 tgtattctct gaataaaata ttaatgatga ttttggtagg ggcattcgca ctaaataatg
     3181 aaaaaataca aatccgagtt tattcctgaa tttaagaaaa attatctttc ccctgtttac
     3241 tggtttacat ggttcgtttt gggaatgatt gcaggtattt caatgtttcc cccttcattc
     3301 agagatcctg tcttggccaa aatagggcgt tgggtgggca gattgagcag aaaagctcgt
     3361 cgcagggcga cgattaattt atcgctttgt ttcccggaaa agagtgatac agaacgggaa
     3421 ataattgtcg acaatatgtt tgccacagca ttgcaatcca tagtgatgat ggcagaactg
     3481 gcaattcgtg gtccggaaaa gttccagaaa cgagtgttct ggaaaggcct cgaaattctt
     3541 gaggaaatcc ggcacaataa cagaaatgtg atttttctgg ttccccatgg ctggagcgtg
     3601 gatattcctg caatgttgct ggcagctcag ggggaaaaaa tggccgccat gtttcatcag
     3661 caacgaaatc cagtgattga ttatgtctgg aattctgtac ggcgtaaatt cggggggcgc
     3721 ttacattccc gggaggatgg gataaaacca tttattcagt cagtacgcca gggatactgg
     3781 gggtattacc ttccagatca ggatcatggt cctgaataca gtgaatttgc tgattttttt
     3841 gcgacctata aagcgacatt accaattatt ggacgtctga tgaacatcag tcaggctatg
     3901 attataccgc ttttcccggt ttatgatgaa aaaaaacatt tcctgactat tgaagttcgg
     3961 ccaccaatgg atgcatgcat tgccagcgcg gacaataaaa tgattgcccg acaaatgaac
     4021 aaaacagtgg aaattttggt ggggtcacat ccggaacagt atatctgggt tttaaaattg
     4081 ttaaaaacgc gcaaatcaaa cgaagcggac ccgtaccctt gaaattataa agtacaggta
     4141 tttccactag ttgtttctta caggttacca atcgaaacac atcccccttc cgtgtgttgc
     4201 ccgtttggca cggaggactg tgtagatgca gttcgttaaa tttaatttat gcctaaatag
     4261 ctgcgcttaa taccgctaca cttttgccag
//
LOCUS       DAETQN010000229         4246 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.232, whole genome shotgun sequence.
ACCESSION   DAETQN010000229 DAETQN010000000
VERSION     DAETQN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.232"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1358
                     /gene="dcuC"
                     /locus_tag="K0W68_004310"
     CDS             <1..1358
                     /gene="dcuC"
                     /locus_tag="K0W68_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBI3075681.1"
                     /translation="VVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSSQ
                     ASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQY
                     INSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPA
                     AIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHI
                     SHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILEF
                     LRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFG
                     SASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASN
                     LGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTG
                     K"
     gene            complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="K0W68_004311"
                     /gene_synonym="citB"
     CDS             complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="K0W68_004311"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HBI3075682.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="K0W68_004312"
     CDS             complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="K0W68_004312"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HBI3075683.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4086..>4246
                     /locus_tag="K0W68_004313"
     CDS             4086..>4246
                     /locus_tag="K0W68_004313"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBI3075684.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGG"
BASE COUNT         1048 a         1079 c          980 g         1139 t
ORIGIN      
        1 gggttgtggt tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg
       61 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      121 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      181 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg
      241 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      301 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga
      361 tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc
      421 cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg
      481 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaag
      541 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
      601 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
      661 atgaaatgtt agatgtcagt gaaatcacca ccactgctcc tgcgttttat gccattttgc
      721 cgttcacgcc gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca
      781 tcatcactat tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct
      841 ttaataccca gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt
      901 ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca
      961 tcggctttat tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc
     1021 tgatgctggt attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg
     1081 cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc
     1141 cggcgtattt gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg
     1201 tttctggcgt agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa
     1261 aacgcacctc ggtgccggtg cttgttggtt tggtgattgt tatcgttgct acagagctga
     1321 tggtgccagg aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt
     1381 tcctccggct ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc
     1441 gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc
     1501 agtggtgcag ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg
     1561 ctctttaaac agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggtagctc
     1621 gtccttaggt tcaccgcgag cataagcatt aaacatctca tcaatttgct tctggctggc
     1681 gctatcaata ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag
     1741 ccgttcatag gcaatgggct taatgagata atcaaataca ccacaacgta cggcttcaga
     1801 caccgtttcc atatcgctgg ctgcagtggt aaacaccacg tcgccgggat aatgcgcctg
     1861 caccagttca tgcagtaaat taatccctct accgtcagga agatagttat cgagcaagat
     1921 tagccccggc ttaaaacgct cgatcatcat tcgggcctgc gccagatttc ccgccagtaa
     1981 tatctgactg aatccgggaa tgtgacgaat atattccgca tgcatctctg ccagcggcgt
     2041 ttcgtcctca acgatcaata gggttaatgg agctgtcatt aggtttcact ttcggaatat
     2101 agattgaaaa taaggtaccg cagggatcat tatcttcgag agtgataaca ccaccgcagc
     2161 gcgttacgta gctggcaatc aagtacaacc caatgccatg ttcaccgggc tcgtcagcac
     2221 gcgtactgac cccctgctca aatattttgt ctcttagaga ctctggaacg ccgcagccct
     2281 gatcggcgac ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgatct
     2341 tgtttccttc atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgccaacaa
     2401 tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga
     2461 cgatgatcat ttttagcccc agttcgcggg cgcgctgcac tttaccaaaa agcagccccg
     2521 ccacctggcg atcggcaaac gcctcacgca ggctgtcaat aagctgttgc tgggcctgag
     2581 actccccctg caccatcgcc agcacgcgat catactcttt catctgcaac agaccattga
     2641 gcgtcgacat ccaattgtga tgctcgtgtc gcaatgtacg aaggctctcg acgtattgtt
     2701 ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc tttactacga aagctgataa
     2761 tggcccccag caaatcatca cctgaacgaa tagcttcccg gttggcaata acgctcagac
     2821 cattaaagtt cgccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg
     2881 cgggcctgac cacttcagca atgggtttac ccaaccattg ccgtccggga gagctcagcc
     2941 ccagcatctt tcttgcgtta cgattgatgg cggtaatgta accatgcgga tccaccgcaa
     3001 tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg acggaccacg cgtgcgattt
     3061 gctttggctc catgcccatc atttgtcgac ggatatgcgc ggccaggaac cacgacagca
     3121 acatcagaat ccctaacagc acgacaaaca cgcctgccat cggtaataaa aactcagccc
     3181 gccagctatc gattttactc accaggtagc caatcgacac cacgccgatg acttttccat
     3241 cgtcatcaaa gattggcgtt ttggcgcgca tcgccatccc cattgcccct ttcccggtaa
     3301 tgaagtagct ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa
     3361 ttttctccgg attaagatgg taaaggcgga tcgagtgccg gtcgccaatc accacataat
     3421 caaaatcggt atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg
     3481 ttttcaccgc agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca
     3541 tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg
     3601 ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgcca
     3661 gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt ttccttgcgg
     3721 gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata catagatatt
     3781 aattatgatc gtggcaaatt ttattcatta aatcaattaa atcacttaaa cctgcaggtc
     3841 atttcgtaat tacattaatt aaaaaactta aaaccattaa ataaataaaa aaccactaac
     3901 tctatgtgaa ataaatcaaa atttcacgct gcaatactcc ttagaatgta tagcgaaagg
     3961 agaaaaagat atacctcgat cacccccctt tctcccaagt gaaaataaaa ggttatcaac
     4021 ttgcaacatt gaacaacatt cgttgcaaat cgataacaac atgcaccttc aggatactat
     4081 ttattatgtt cggcaatgat attttcaccc gcgtaaaacg ttcagaaaat aaaaaaatgg
     4141 cggaaatcgc ccaattcctg catgaaaatg atttgagcgt tgacaccaca gtcgaagtat
     4201 ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg tggaat
//
LOCUS       DAETQN010000230         4198 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.233, whole genome shotgun sequence.
ACCESSION   DAETQN010000230 DAETQN010000000
VERSION     DAETQN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.233"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1361)
                     /locus_tag="K0W68_004314"
     CDS             complement(<1..1361)
                     /locus_tag="K0W68_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBI3075685.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1426..1545)
                     /locus_tag="K0W68_004315"
     CDS             complement(1426..1545)
                     /locus_tag="K0W68_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBI3075686.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1700..1918)
                     /locus_tag="K0W68_004316"
     CDS             complement(1700..1918)
                     /locus_tag="K0W68_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3075687.1"
                     /translation="MKKLLRTLSAVDARVVCKPHRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
     gene            complement(2272..3750)
                     /locus_tag="K0W68_004317"
     CDS             complement(2272..3750)
                     /locus_tag="K0W68_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HBI3075688.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKVESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWRTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1184 a          920 c          739 g         1355 t
ORIGIN      
        1 actaaagcac tgcccgtctc ctggttaacc ccatgtgcta taccgccggt atagacaatc
       61 agcgagcctc cggcgctaat attgctgcca gttgccgtac catctttctc aacaacctgc
      121 cggctcccgg tggaaatatt tgtcgtgtca gctttcccgc cgctcttgat attttgtgtt
      181 ccgctgttga tattggtacc cgtggcaata ccatgattat taatattctg tgtaccacca
      241 ttgattatgg tattggtcgc gtttctggta acatccatta ccccgccgtt atctattcgg
      301 gtcgcatcag ctttagcatt cgttaaaact gacattgttc ctttatcttt aataatcgtc
      361 ttatttgccg aaccatatgc gtttatgtct aaatgaccac cgttttccag caacacattg
      421 tctgccacgt gattgtggat ggagaatgca ccttcactat tcgtaccgct caccgtcgta
      481 ccgttagtgt ttgtttttaa aattgcacca tcgtgctggg taacatttgt tgccgtacca
      541 ccactaacat caagcacgcc accggaataa acttcaataa catccgagga gctggtgtaa
      601 tcaacaattt gcgtaccacc agaatagatc tgagtatttt ttgccgttga cttattattc
      661 agagactgag ttccaccttc aatcgtcgtg tccagcgcat ggctttcata tactctttgc
      721 tcaccgccat ttttaatggt ggttgttcct actgtgctct gttcaacata ctgtcgtcca
      781 ccatttatga ttgtgttcgt tgccatactt ccttgcgtga tgtcctgata accggactta
      841 tttatcgttg taccatcaac atgcccctga gttgttactg tctgccctcc accatcaatg
      901 atggttccat tcgcagtccc cccacttatg tgtgaattac cacccttaat tgtcgttcca
      961 ttgctgatac cgcctgtata aacgtcctga ttgccactgt cgattatcgt accggtggaa
     1021 atgcccccgt catgaattgt ctgtcttcct ccgttaatgg ttgtattatt agactgcccc
     1081 atataattgt tattccttcc aacatcttga tatccaccag attcaataag acttccatta
     1141 gatactccgc cataaacact ctgctggcca tggttgataa tatgagtgtt atttgttgta
     1201 cctcgttcat ctactcgttg gttgccatct acagtctcat cgtttaccac accaataaca
     1261 ttagtagtaa aggccgccat cacgggcggg gcatatatca aggcagatat caataaggaa
     1321 agtactgagc ggcgacaata atggggactg gtcctgttca taaatttcat cctctgaaaa
     1381 gtgaatactg agtagcgttt aagcgacttt agctctgctg caacatcagc ccgcaggcac
     1441 cagaccgggg ggttcatcct gaagagacag cgcaagtgta ttgtgttcac cgctcatcaa
     1501 agacatcatg atgaaatgat gatgttccac ataagaaaga ggcatttttt aaacacagtg
     1561 cgctgaagtg tgattggata aaaaagtcaa tctattcagg aaatacgggc gtattctttt
     1621 ctttcgacaa tcaggccgtc ggcaaaataa aatgatttac ataatcgttt ctgatgaata
     1681 tcttctgctc acataaaaat cacacaataa ctttgagatc gcagattctt ttacttttac
     1741 agcattcgtc cccccattgt tgggcaaata tagattgggc cagagcacga aagttaatac
     1801 cacgtttgca cagctcctcc aacagcacga caagatgcca catactgcgc cccagtcggt
     1861 gcggtttaca gactacccgt gcgtccaccg ccgataatgt cctgagcagt tttttcagtc
     1921 cggacctcct gccacggttt tacgttgaag aggtaaccct gagcacgcag ttcttcagtc
     1981 aggcgtggtg caccgtaacg ctgttattgc tgggtaagat caaaaaactt tcaggcagct
     2041 aaggaaagtt gaaccagaca ttagatgaaa tatttcaacc aattacagca ccaatttaaa
     2101 ctcagtaaat cacagcacag atcaaggggt tacgtgaaag cgtagcccct ttttctttgg
     2161 tagtggcagc aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca
     2221 tattgtttca atctacgtta ttatctcttt gtaaaaatag ccatttatta atcattgaaa
     2281 actgctttta gaacttgata caacgggact agtcacaaca ggactattct caacgggatc
     2341 atcctcagag gaactatcat caaagtcatc atccataaat aaaatatcat cgaatggtgc
     2401 cacgcccgtg atgagtttta ttttattatt acgatcagtc aagactccac ttaaaccgtt
     2461 ttcgctcaca ggttttaatg atttttcatt actcttgttg taagcaggcg cattaaaaat
     2521 acacggagta tcaagatcaa acaatgacgt tctccagttc acatattgaa tatcatagtt
     2581 actgaagttc tgtccagaaa agaagcatcc cttaaaatcc aatccaccta aattatataa
     2641 accatcctct tctttttgga gagtaatgtt aattctggct atctcccgga caaaacttcc
     2701 ttttttgtat ttgaataccg tttcaagatt ttcaccagac agcttgactt ctgggaataa
     2761 tttaaaatta aaccctacat cattattatt caaagtagat gaacgaaaga tggaaaaagc
     2821 ttgtagtgct gaattatagc tttcgacttt atcttgaggc tgcgctcttg gtaaaaactt
     2881 atagcagcac tcataaaaat tgaataaacg ttctgaacaa ttcccatttt tatcaattaa
     2941 taccccccgg aattctttca caaaggcatc cgttattttt tgaatatcta tggtgtgttc
     3001 ctttgactct gacaaagatg gagtctgttg aagtgcattg ctcatctggt taaggaacat
     3061 ctgcatttct ttaaatttat ctccatagtg gcctttaact tgctgagagt ttttagttac
     3121 aggtgaaacc ttatctattt ggttgtcata taaattatgt ggttccatac agacatttgg
     3181 ttgtgtttct aaaataacac tacgtgtact acctggctta aatgaatcaa catgaaaatc
     3241 actttttctt atattatcga atagggtctg ttcatttctc ttagcgcatt caataaactg
     3301 atcaacatta tttttattcg atgacaggtc agtttcagaa accgcatttt ctttgttctc
     3361 cagctttagt ttaatgagaa gatcttccca gacctgcttg cttaaacata ctacgtcagg
     3421 ctcaccatca gtaactaact cttcattatt atcaaagtat acgttgaata cttttaagtt
     3481 attttcatca acatcatatt taatacgttt taattgctct ccagatccca taatgacaaa
     3541 ggcgttgcct tcatgatata tacattcaga catcattttt tgtaaagttt caagagcacc
     3601 gcgatacgtc cctgaggcag cttccttaca aattaaattt ataatactat aagctagtag
     3661 ctccatggat tcagaagatt ttctttcatt acaatttcca ctatctataa atgagctagt
     3721 gttaaattcg ttgcttttac taactaacat tgtctattcc tcagttaatg tctacatggc
     3781 tatttttaat tttattattg tttgtcacta taaaaaatcg ctcatttgag acaattgctg
     3841 gcattaacag cttcatatgc tatacatggt acttttaatt aaattagcac aagaatgtta
     3901 aatttaataa acaaaaagtt atttcgctgt atgataaaaa ccacccgtta taatttatta
     3961 gtgaaattcg tttttcgagt gttagaaatt tatatctaaa tagcgttgat taatgagcaa
     4021 agccagactc ctgtaacgct cacaagactc atctacctgc gacgggtatt ctggtcagta
     4081 gtagatgttt aaggcgtggc agagacattt catccttact ctacggcatt ggcccgcata
     4141 caaatcgtgg ttgtgacact cacttatcat cagtgagcga acagagaata gttcagtg
//
LOCUS       DAETQN010000231         4192 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.234, whole genome shotgun sequence.
ACCESSION   DAETQN010000231 DAETQN010000000
VERSION     DAETQN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.234"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="K0W68_004318"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="K0W68_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBI3075689.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="K0W68_004319"
     tRNA            complement(330..405)
                     /locus_tag="K0W68_004319"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="K0W68_004320"
     tRNA            complement(412..486)
                     /locus_tag="K0W68_004320"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="K0W68_004321"
     tRNA            complement(603..687)
                     /locus_tag="K0W68_004321"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="K0W68_004322"
     tRNA            complement(696..771)
                     /locus_tag="K0W68_004322"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="K0W68_004323"
                     /gene_synonym="panK"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="K0W68_004323"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBI3075690.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="K0W68_004324"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="K0W68_004324"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBI3075691.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="K0W68_004325"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="K0W68_004325"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBI3075692.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1040 c          936 g         1136 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatct tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 acttcccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAETQN010000232         4186 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.235, whole genome shotgun sequence.
ACCESSION   DAETQN010000232 DAETQN010000000
VERSION     DAETQN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.235"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..324
                     /locus_tag="K0W68_004326"
     CDS             <1..324
                     /locus_tag="K0W68_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3075693.1"
                     /translation="FYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYS
                     AGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKEN
                     VAPGG"
     gene            551..1816
                     /locus_tag="K0W68_004327"
     CDS             551..1816
                     /locus_tag="K0W68_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3075694.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1828..2694
                     /gene="ydiB"
                     /locus_tag="K0W68_004328"
     CDS             1828..2694
                     /gene="ydiB"
                     /locus_tag="K0W68_004328"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBI3075695.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2725..3483
                     /gene="aroD"
                     /locus_tag="K0W68_004329"
     CDS             2725..3483
                     /gene="aroD"
                     /locus_tag="K0W68_004329"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBI3075696.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            3627..>4186
                     /locus_tag="K0W68_004330"
     CDS             3627..>4186
                     /locus_tag="K0W68_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075697.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIR"
BASE COUNT         1092 a          938 c         1005 g         1151 t
ORIGIN      
        1 ttttatgtgg tgataatatt tgcttttgtc attggtttta cctctgctgg aggtgttgtg
       61 caaattggcc tgacgttaat ggctgaacgt ttcccttacg ctaaaggtaa agctacaggg
      121 atctattaca gtgcgggcag tattgcgacc tttactattc cgttgattac ggctcatctg
      181 tcccaaagaa gtattgccga tattatgtgg ttcgataccg ccatcgctgc catcggtttt
      241 ttactggcac tgtttatcgg cttacgcagc cgcaaaaaaa cgcggcatca ctcgctaaag
      301 gaaaatgtcg ctccgggtgg gtaatgcaat attcttttca ggtcatgcaa gatcttacgg
      361 ataaataact ctttctgcgc taactaagga aaatcgcgat caaaaacaaa ctatgacatg
      421 caatattcct ggaaacataa actttatgcc atgtacccag ggaaaatcat cttcagtata
      481 gtaattatgt aaaccgtcgg agaacaatac gtacggtaac gaaattatct ttcagcaagg
      541 agctgtgaaa atgtctcaaa ataaggcttt cagcacgcca tttatcctgg ctgttctttg
      601 tatttacttc agctatttcc tgcacggcat tagtgttatt acgcttgccc aaaatatgac
      661 atctctggca gaaaagtttt ccactgacaa cgcgggcatt gcctatttaa tttccggtat
      721 cggtttgggg cgattgatca gtattttatt cttcggtgtg atctccgata agtttggtcg
      781 tcgggcggtg atattaatgg cagtaataat gtatctgcta ttcttctttg gtattcccgc
      841 ttgcccgaat ttaactctcg cctacggtct ggcagtgtgc gtaggtatcg ctaactcagc
      901 gctggatacg ggtggctacc ccgcgctcat ggaatgcttt ccgaaagcct ctggttcggc
      961 ggtcatactg gttaaagcga tggtgtcatt tgggcaaatg ttctacccaa tgctggtgag
     1021 ctatatgctg ctcaataata tctggtacgg ctatgggctg attattccgg gtattctgtt
     1081 tgtactgatc acgctgatgc tgttgaaaag caaattcccc agccagttgg tggacgccag
     1141 cgtagctaat gaattaccgc aaatgaacag caaaccgtta gtctggctgg aaggtgtttc
     1201 atcggtactg ttcggtgtag ccgcattctc gaccttttat gtgattgtgg tgtggatgcc
     1261 caaatatgcg atggcttttg ctggtatgtc agaagctgag gcattaaaaa ccatctctta
     1321 ttacagtatg ggctcgttgg tctgtgtctt tatttttgcc gcactactga aaaaaatggt
     1381 ccggcccatc tgggctaatg tatttaactc tgcgctggca acaataacag ctgccattat
     1441 ctacctgtac ccttctccac tggtgtgtaa tgccggcgcc tttgttatcg gtttctcagc
     1501 agctggcggc attttacagc tcggtgtttc ggtcatgtca gagtttttcc ccaaaagcaa
     1561 agccaaagtc accagtattt atatgatgat gggtggactg gctaactttg ttattccgct
     1621 gattaccggt tatctgtcga acatcggcct gcaatatatc attgttctcg attttacttt
     1681 cgcgctgctg gccctgatta ccgcaattat tgtttttagc cgctattacc gcgtttttat
     1741 tattcctgag aatgatgtgc ggtttggcga acgcaaattt agcacccggt taaacacaat
     1801 taagcataga ggttaaagga gttaattatg gatgttaccg caaaatacga attgattggg
     1861 ttgatggctt atcctatccg ccacagttta tcgcctgaaa tgcagaataa agccttagaa
     1921 aaagcgggat tgccatttac ctatatggcc ttcgaagtgg ataacgatag ctttcccgga
     1981 gcaattgaag gattaaaagc cctcaaaatg cgcggaactg gtgtgtcgat gccgaacaaa
     2041 caactggcgt gtgaatatgt tgatgaatta acgccagcag ccaaactggt gggtgccatc
     2101 aacaccatcg ttaatgatga tggctatctg cgtggctata acaccgacgg cacgggccat
     2161 attcgcgcca ttaaagagag cggttttgat atcaaaggca aaacgatggt gctgttaggg
     2221 gccggaggtg cctcaacagc gattggcgcg cagggggcaa ttgaaggttt aaaagaaatt
     2281 aaactcttta accgtcggga tgagttcttc gataaagccc tcgccttcgc gcagcgggtt
     2341 aacgaaaaca ccgattgtgt cgtcacggtt accgatctcg ccgatcagca agcctttgct
     2401 gaagtcctgg cttccgccga cattttaacc aatggcacaa aagtaggtat gaaccccctt
     2461 gagaatgaat cattggttaa tgatatcagt ctgttacatc cgggactgct ggtcactgaa
     2521 tgcgtgtata acccgcatat gacgaagtta ttgcagcagg cacaacaagc aggttgcaaa
     2581 acgattgatg gatacggcat gttgttgtgg caaggggctg aacagttcac gttatggact
     2641 ggcaaagatt tccctctgga atatgttaaa caggtcatgg ggttcggtgc ctgacaggct
     2701 gaccgcgtgc agaaagggta aaaaatgaaa accgtaactg taaaagatct cgtcattggt
     2761 gcgggcgcac ctaaaatcat cgtctcgctg atggcgaaag atatcgcccg cgtgaaatcc
     2821 gaagctctcg cctatcgtga agcggacttt gatattctgg aatggcgtgt ggaccacttt
     2881 gccgacctct ccaatgtgga gtctgtcatg gcggcggcaa aaattctccg tgaaaccatg
     2941 ccagaaaaac cgctgctgtt taccttccgc agtgccaaag aaggcggcga gcaggcgatt
     3001 tccaccgagg cttatattgc tctcaatcgt gcagccatcg acagcggcct ggttgatatg
     3061 atcgatctgg agttatttac cggcgatgat caggttaaag aaaccgtcgc ctacgcccac
     3121 gcgcatgatg tgaaagttgt catgtccaac catgacttcc ataaaacgcc ggaagccgaa
     3181 gaaatcattg cccgtctgcg caaaatgcag tccttcgacg ccgatattcc taagattgcg
     3241 ctgatgccgc aaagtaccag cgatgtgctg acgttgctta ccgcgaccct ggagatgcag
     3301 gagcagtatg ccgatcgtcc aatcatcacg atgtcgatgg caaaaactgg cgtaatttct
     3361 cgtctggctg gtgaagtatt tggctcggcg gcaacttttg gtgcggtaaa aaaagcctct
     3421 gcaccagggc aaatctcggt aaatgattta cgcacggtat taactatttt acaccaggca
     3481 taagcaataa tatttcggcg ggaacaccct ccccgccgaa cttaaaaaat atatttaacc
     3541 gtatttaata aaaatatttc gtgagtctct gtgcgctaat tctccatctg gcgtagggaa
     3601 aatcacatct gaatcaggaa ttaacaatga aacctgtaaa accacctcgt attaatggac
     3661 gagtgccggt cctgtcggca caggaagcgg tgaattatat tcccgacgaa gcaacacttt
     3721 gtgtgttagg cgctggcggc ggtattctgg aagccaccac gttaattaca gctcttgctg
     3781 ataaatataa acagactcaa acgccacgta atttatcgat tattagtccg acagggcttg
     3841 gcgatcgcgc cgaccgtggt attagtcctc tggcgcaaga aggtctggtg aaatgggcat
     3901 tatgtggtca ctggggacaa tcgccgcgta tttctgaact cgcagaacaa aataaaatta
     3961 ttgcttataa ctatccacaa ggtgtactta cacaaacctt acgcgccgcc gcagcccacc
     4021 agcctggtat tattagtgat attggcatcg ggacatttgt cgatccacgc cagcaaggcg
     4081 gcaaactgaa tgaagtcacg aaagaagacc tgattaaact ggtcgagttt gataacaaag
     4141 aatatctcta ttacaaagcg attgcgccag atatcgcctt cattcg
//
LOCUS       DAETQN010000233         4124 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.236, whole genome shotgun sequence.
ACCESSION   DAETQN010000233 DAETQN010000000
VERSION     DAETQN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.236"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..83
                     /locus_tag="K0W68_004331"
     CDS             <1..83
                     /locus_tag="K0W68_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI3075698.1"
                     /translation="FWHDLAALVIAGILASMIVNWLNKRK"
     gene            459..566
                     /locus_tag="K0W68_004332"
     CDS             459..566
                     /locus_tag="K0W68_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI3075699.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(653..2332)
                     /gene="bcsG"
                     /locus_tag="K0W68_004333"
     CDS             complement(653..2332)
                     /gene="bcsG"
                     /locus_tag="K0W68_004333"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBI3075700.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2329..2520)
                     /gene="bcsF"
                     /locus_tag="K0W68_004334"
     CDS             complement(2329..2520)
                     /gene="bcsF"
                     /locus_tag="K0W68_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBI3075701.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2517..4088)
                     /gene="bcsE"
                     /locus_tag="K0W68_004335"
     CDS             complement(2517..4088)
                     /gene="bcsE"
                     /locus_tag="K0W68_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBI3075702.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1051 a         1029 c         1074 g          970 t
ORIGIN      
        1 ccttctggca tgatttagcg gctctggtca ttgctggcat tcttgccagt atgatcgtga
       61 actggctgaa caagcggaag taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt
      121 tgcgcccgga ccaggccgca ggggtgaaac tctgcggcct ttttcgtaag aaaaatgcaa
      181 cgattgcacg cggataatat tctgcggaat gcgcagcaca gagtgaagac gtgacggatt
      241 ccggaacaga aaaacagtcg tagaatcagc ggcaagccgg aaaggttccg gtgaggcgca
      301 atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa cccccgcacg
      361 ttgcaggtat gcacctgaca acatcacggg ggctaatctt gaacccaaac aactcaagaa
      421 tagccgcgaa acgttggcat tacaacacag gcggctatat gacgctcgca gaactgggca
      481 tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc agtatgatcg
      541 tgaactggct gaataagcgg aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca
      601 atttgcgccc ggatcaggcc gcagaggtta aactctgcgg cctttttcgt gcttactgcg
      661 ggtaaggcac ccagtcgccg ccgttcaggc gaacgtacgg tttatcctgg tattgaataa
      721 ctactgcatt tgagttctcg gagaccggtg ctgtttgtgg caacccactg gtgagttttt
      781 tccagtcaac attgtcttcg gtgaaaatct tgccatcaag aacgcgaacc accagatcgg
      841 agatagctag gaagctgctc ggttggtcaa tgacaatcgg tgccccctga tgtggtgcct
      901 tcatgccgaa gaatttcacc ccaacgggga cgtctgtgat agacgggcta gggatatcac
      961 gtaggccaga tacctgcatt ctgtcgccct tcagcgcgcc gccgtgttcc ggcaccacga
     1021 ccaccatcac tttacgaccc gatttctcca gttcagtaaa gaaggcgtcc agttcatcaa
     1081 agaatttctg cgcccgcgct ttgtaatccg ctgttttgct gacccccgga taatggttgc
     1141 cgtcatgcag tggaagcgtg ttgtagaacg tggcactacg gctgttttta tctttttcgg
     1201 taacgtccag ccagcggtta agcacggcgg tatcgtcata aaccggcgaa ccatcaaagc
     1261 ccagcaaaat aaccggcaga tttgtttgat ccatcaattc agtctgcatg ccgccatttt
     1321 cgcgaacttc tttcaaaaaa ccgccgaact ggccgttatg tcccatcatc aggtgctggg
     1381 taaagcccag tttcgaaagg ttatcaaaca gatagcagtc gttatttgcc ggttgataca
     1441 gattagtgtg cgaagtctgc ccgcagctgg cgcgcagtaa acggatcgcc gccgggccac
     1501 tgtaggaggt ggcggagtta aagttcttga actcaatatc gaaatgcgac cacagtggat
     1561 gcgacatcaa cccggcggct tctatatccg accaggaaag cgaacagatg ttaatcacca
     1621 gtagttcaaa tggctgagca tcagcgggca gcgaagacgg gaaggtcgat ttacgtttcg
     1681 cctccgcgtt atagaaatta ttcagccagg cgttaaggtt cgccgttgtt ggcggtgcag
     1741 tttgtgcggg catatcaccc actaccgatg cgccacccgt cgccgcaacg gttgccgctg
     1801 cgttaccacc cgtcgttgtt acagtggtcg tcggttgtcc ggctggccac aaggagaaac
     1861 ttggtcccgc cagggtaagt acgttcagcc atagcagtat ggcaaccaca aaaacggtaa
     1921 tgcgaatcca ttgtgacagg aataaccagg ccactaataa aacaaaaatg gccccaatca
     1981 tctgccagtt aataaagcgt gtgacaaggt cgattaaata atcggtactg aaccccgcca
     2041 cctgcgaacc ctggctcatt atgctttccg ggccaggcaa ccaggtgtca tgccagaaca
     2101 aagcaaagcc gatcggcagg gcaatccagt ggcgcaagcg atgcaggctg tagcgcggaa
     2161 tgggcatcag cagaaacgtg gcaaacacca aattgaggag cggatggaag ttaagatatc
     2221 ccgcccacaa caggccgaac ttaaccagaa aatagaagtt ccagccggaa aggccgcgcc
     2281 agtattgcca gagggaagaa ggcatggcgg tattttgcgt aaattgagtc attttttggt
     2341 tgccctggct ttttccgtgc ggcgtaacgt cccggccggt ttaacataac gaggtttagc
     2401 aaagaataaa cgtaaggtgt cgcgaatgcg tcgcaaaaag tgccgcgcca gatagcccag
     2461 cgggaaaaat atcagtgcgc aaacgacaat aatttcaatg atatcgctga tggtcatcat
     2521 gatgagcgct ccacagcatc atctaacagt cgcatgggtt ctggtattcg tcgccagtgg
     2581 cgaccatcgt gctcggcatt gatgaccggt ttagaacttt gcgttaaagg cagcggcatg
     2641 ccccattgtt ctggggcaag caagcgcatc tgcaccagct cggcactgat ttgatcatct
     2701 tcaaaccaga ccatacggtt tgagaaaatg tcgccagtag gcaatgggaa aatatgattc
     2761 aacgcggtat ccagatcgtt aatccgacag aatgagagaa acagcaccag ccgattaccg
     2821 ccaatggtca tgatatcgcc ggtacggtta gggcgacaca gcgtcagggc ttgttcaacg
     2881 cggatacccg gtaccggacg tagggcaacc agaacgcctt taccgtgggc aggtaataga
     2941 gggttattca tcatgttgtt gacggcatta cagaacacat cccacttctg gaaaccacgc
     3001 agtttgagcg gctgggtcat tgacagcaag gtagtgatat cttccggcac atagcgacta
     3061 aacttctgcc cttgcacgct ttcgatcatc gtcagacaac gggagagtgg cgcattccac
     3121 ggaataacca tatttgcacc gcaggccaat aacaaacgtt catcggtggc gcgcaggcta
     3181 gcggtatttt cccgcacgag gattttcatc gcactaccgc gctggcgacg cagggtatga
     3241 atgctgcggg ccagtggctc gatttgcgca ttttgctgta aagaaaagac caccgtcgcc
     3301 gcctgagcgg tacgggcttc attgaacagg acttcgttat tgttgaacag ttgccagtgt
     3361 tccgatagcg gcggcgcacc ttccagtaca gcaacattac tcagaatgcg tttttcgtcg
     3421 ctgcgtggtt ggatctccgc ctcttcgctt tgaactaatg tccagatacc attttgttgc
     3481 tgaacgctaa gctgctgacg ggcgctgacc cctttttcgt tgcaccagaa ggcaatatcc
     3541 agcaaatgtt ggtcaccctg aaaacgcaaa ctggcaagac caaaaagtga acggtattcc
     3601 tcaagcaaca atgaaaattg tttatcgtta ttatttccgg gattaattac caaaagcgaa
     3661 caatggttta acctgctcca tttattcatt ttatccaacc atgagcgaag ccgctcggca
     3721 ggaatgtttt gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt atggggatca
     3781 atagaacact gcaaatcacg ggccaaatag tatagacctt tttcatgatt tagcattgaa
     3841 aataatttta ttttttccgg cccttgagaa tcatctaatt gaaagatttt cgccggatcg
     3901 ctgtccatgc taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat tgtttgattc
     3961 gccagactga tagcatcttc atggcgatcg acgttaaacc accagacgcc gcctgctggc
     4021 atatgtcgca gctcatccca taatgatgag ataccgatag agaatacagg gtccacaatg
     4081 tccctcatgt caccattttg tctgattatc agtttactag cgaa
//
LOCUS       DAETQN010000234         4114 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.237, whole genome shotgun sequence.
ACCESSION   DAETQN010000234 DAETQN010000000
VERSION     DAETQN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.237"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..118
                     /locus_tag="K0W68_004336"
     CDS             <1..118
                     /locus_tag="K0W68_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3075703.1"
                     /translation="ISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            complement(161..1117)
                     /gene="ydcO"
                     /locus_tag="K0W68_004337"
                     /pseudo
     CDS             complement(161..1117)
                     /gene="ydcO"
                     /locus_tag="K0W68_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
     gene            1170..1706
                     /gene="sutR"
                     /locus_tag="K0W68_004338"
     CDS             1170..1706
                     /gene="sutR"
                     /locus_tag="K0W68_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HBI3075704.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1779..3740
                     /gene="rlhA"
                     /locus_tag="K0W68_004339"
     CDS             1779..3740
                     /gene="rlhA"
                     /locus_tag="K0W68_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HBI3075705.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3832..4062)
                     /locus_tag="K0W68_004340"
     CDS             complement(3832..4062)
                     /locus_tag="K0W68_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HBI3075706.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1072 a         1048 c         1034 g          960 t
ORIGIN      
        1 catctcagta atacgggaat acattgagat tttctacaat cgtcagcgtc gtcactctcg
       61 tctggggaat atctccccgg cagccttcag ggaaaaatat catcagatgg ctgcttaaaa
      121 aaagaacaaa tggtagtgtc cgctattgcc agtacacctc actaccaaac agacctgcga
      181 gcaaatagaa aatgcctgca acggcagctg ccagccaacg ttgatcttta tccggatgcg
      241 cttccgggct ttggcaaata gccgcggtga ttgccgcaat accgacggaa taaacgccga
      301 aaggggaaaa aaccagtgcc agcaatccag taaatacaat taatggcgaa acaggagccg
      361 aatatccagc tgctttcatt gctgcgatac ccggtgcgtt ttgcgatgcc atcgtcacca
      421 gaaaaagggg gagtgcaacg ctcaggctgt gagcaaacga aaaatcaggg gtaatataag
      481 tggggagaac gggtttaaag acaacatcag ttgtgacaac gtcaccttgc gcgataacga
      541 tcacgatccc aataatcatc gcggcaatta ccgcatagcg cggcgcaacg gccttggttg
      601 ccagccatac cagcaacata cttccacaca acgtaaattg accgtccaga ctggcaaacg
      661 cctgtaaacc aaagcgtaat aaaatcccgg caagcattgc cgccgcaagc gagtgcggaa
      721 taatgcgcat cagacgagca aagagtcccg ttatgccgca gaggactatt aacgcgttgg
      781 tgacaataaa aacgccgatg gcttcgttaa gtgttagtcc ctgcaatccg gtgaccaaca
      841 aagccgcgcc aggcgttgac catgcggtga gaacaggtac gcgataccat aatgtcagag
      901 tcagcgtact gacgcccatt gccagcccca gcgccgtcat ccagccagag atttgtgcag
      961 tggtggctcc ggcgacaatc gctgcttgcc agattattgc cgctgaactg gcgtagccaa
     1021 ttaataccgc cagaaacccc gccagtagcg tgggtggagg aatagaaaac agacgcatag
     1081 tcacctcgtg cgttatagcg tacaggcacc gtagcatttg tccgttataa cgcacaagtg
     1141 ataaacttcc gttttgccgg aggagtcgca tggaaaatct cgctcgcttt ttatccacta
     1201 cacttaaaca actacgccag cagcgcggct ggagtctttc gcgactggca gaagcgacgg
     1261 gcgtttctaa agcaatgtta gggcagattg agcgtaatga gtccagtccg acggtcgcga
     1321 cattatggaa aattgccacc gggttgaacg taccattttc aacatttatt tctccgccgc
     1381 agtccgctat gccttcagtt tatgatccac aacagcaggc gatggtgatc acttcgctgt
     1441 ttccttatga tccacaactc tgctttgaac acttctcgat tcagatggca tctggtgcaa
     1501 taagcgaatc gacgccccat gagaaggggg taatcgaaca tgtcgtcgtc attgatggac
     1561 aacttgatct gtgcgttgat ggggaatggc agacccttaa ttgtggggaa ggcgttcgat
     1621 ttgctgcaga cgtcacgcat atctatcgta atggtgggga gcaaaccgta cattttcatt
     1681 ccctcatcca ttacccgcgc agttaagcgg gaaaactatt tcgcaacgtc gcgcttgtgg
     1741 ctaaaatagc cgccattttt cagctactgg ataagaatgt gaccgtatct tctcatcgac
     1801 ttgaactgtt aagcccggca cgcgatgccg ccattgcccg cgaagctatt ttgcacggtg
     1861 ccgatgctgt ttatatcggc ggccctggtt ttggtgcccg tcataatgcc agtaatagcc
     1921 tgaaagatat tgccgagctg gtgccgtttg cccatcgtta tggtgcaaaa attttcgtca
     1981 cgcttaacac cattttgcat gatgatgagc tggaacccgc gcaacggctg attactgacc
     2041 tctaccagac cggtgtcgat gcgctgattg ttcaggatat ggggattctg gaacttgata
     2101 ttccgccgat tgaactgcac gccagtacgc agtgcgacat tcgtacagtt gaaaaagcga
     2161 agttcctctc tgatgttggc ttcacgcaga ttgtgctggc gcgagagctg aatcttgatc
     2221 agatccgcgc gattcaccag gctacggacg cgaccattga attctttatt catggggcac
     2281 tgtgcgtggc ctattcgggt cagtgctaca tttctcatgc gcaaacaggg cgtagcgcca
     2341 accgtggcga ttgctcgcag gcgtgccgtt tgccatacac attgaaagac gatcaggggc
     2401 gggtggtttc ctatgaaaaa catctgctgt cgatgaaaga taacgatcag actgccaacc
     2461 tcggcgcgct gattgatgct ggcgtacgct ccttcaagat tgaagggcgt tacaaagata
     2521 tgagctacgt gaagaatatc accgcccatt atcgccagat gctggatgcc attattgaag
     2581 aacgtggcga tctgacgcgt gcttcatcag gtcgtactga acatttcttt gttccatcga
     2641 cggaaaagac tttccaccgt ggtagcacag attattttgt gaatgcccgt aaaggcgata
     2701 ttggcgcgtt cgattcgccg aaatttatcg gcctgccggt gggtgaagta ttgaaagtgg
     2761 cgaaagatca tctcaatgtt gccgtcaccg agccactggc aaatggtgat ggcctgaacg
     2821 tgatgattaa acgtgaagtc atcggttttc gtgccaatac ggtcgagaaa accggagaaa
     2881 atcagtaccg cgtctggccc aatgaaatgc cagcagattt gcacaaaatt cgtccacatc
     2941 acccactaaa ccgtaatctt gatcataact ggcagcaggc actgacaaaa acctccagcg
     3001 aacgtcgggt ggcggtagac attgaactgg gcggctggca ggaacaactg attctgaccc
     3061 tcaccagtga agagggtgtc agcatcacgc atacgctgga cgggcagttc gacgaagcca
     3121 ataacgccga aaaagcaatg aacaatctga aggatggtct ggcaaaactg gggcaaaccc
     3181 tctattacgc ccgcgatgtg caaattaatt tgccgggggc gctgtttgta ccaaacagtc
     3241 tgttaaacca gttccgccgt gaagctgctg acatgctgga tgctgcgcgt cttgccagtt
     3301 accagcgcgg cagccgtaaa ccggttgctg atcctgcgcc ggtttttccg caaacgcatc
     3361 tgagtttcct cgcgaacgta tacaaccaga aagcgcgtga attttatcat cgctatggtg
     3421 tgcagctgat tgacgcggcg tatgaggcac atgaagagaa gggcgaagtc ccggtgatga
     3481 tcaccaagca ttgtctgcgc tttgccttta atctgtgccc gaaacaggcg aaaggcaata
     3541 tcaaaagctg gaaggcgacg ccaatgcaac tggttaacgg cgatgaagta ttaacgctaa
     3601 agtttgattg ccgcccatgc gagatgcacg tcattggcaa aatcaaaaat cacatactga
     3661 aaatgccgtt accgggaagc gtagtggcat ccgtaagtcc ggatgagctg ctgaaaacat
     3721 tgcctaagcg aaaagggtaa aacgccagtt ttctggttac tcacaactca ttgaatctgc
     3781 atgatattgc ctgccgggta aggcgtcacg ccgcatccgg catcaaacga ctcaacgttg
     3841 tcccgcttct ggtttgcgcg atttttgcca gtaaaaatgc tcgcgcaaac cttccgccga
     3901 ctcttccgcc acagcacgca attcatcgct gtccgcttca tgacgcagct gatgatccac
     3961 attctttacc cacacaaatt catgtccttt gtgccctgcc atgagttgtc ctgagaacag
     4021 cgcacacgtt aataagacaa ccgataacgc cttcgtaaac atcctgccac ctttttctta
     4081 ccttttgccg ctatgatgcc gatcgtttct tgag
//
LOCUS       DAETQN010000235         4107 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.238, whole genome shotgun sequence.
ACCESSION   DAETQN010000235 DAETQN010000000
VERSION     DAETQN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.238"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..759)
                     /gene="nlpA"
                     /locus_tag="K0W68_004341"
     CDS             complement(<1..759)
                     /gene="nlpA"
                     /locus_tag="K0W68_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HBI3075707.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVALGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
     gene            981..1274
                     /locus_tag="K0W68_004342"
     CDS             981..1274
                     /locus_tag="K0W68_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075708.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(1315..2495)
                     /gene="nepI"
                     /locus_tag="K0W68_004343"
                     /pseudo
     CDS             complement(1315..2495)
                     /gene="nepI"
                     /locus_tag="K0W68_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2706..3158)
                     /locus_tag="K0W68_004344"
     CDS             complement(2706..3158)
                     /locus_tag="K0W68_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBI3075709.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(3211..>4107)
                     /gene="adeQ"
                     /locus_tag="K0W68_004345"
                     /gene_synonym="yicO"
     CDS             complement(3211..>4107)
                     /gene="adeQ"
                     /locus_tag="K0W68_004345"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HBI3075710.1"
                     /translation="LESLPGIGLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGI
                     LGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSG
                     ALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAG
                     AFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALI
                     FVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRW
                     RDLNLCVVVVASLFALKIILVD"
BASE COUNT         1211 a         1080 c          911 g          905 t
ORIGIN      
        1 tgattgataa gattgcagaa attccttcac gttttctgca ttcttattat cttcccgtgc
       61 caccaaaata ttcacatacg gcgaattctt atcttcaata aatacgctgt cgtgcaccgg
      121 agaaagccct gtctgctgaa tgtaagtggt gctgataatc gctacatcaa ctttgggatc
      181 atccagtacg cgcgggagct gcgccccctc gagttccata atctgcaaat gacgaggatt
      241 atcggttata tccagcgcgg taggtaataa cccttttccc tctttcagag taattagctt
      301 ctctttttgc aacagtaaaa gtgcgcgacc aaggttggtc gggtcgttgg ggatcgccac
      361 tgtcgcgcct tcttttattt gcgccacggt tttgattttt ttggaataac ccgccatagg
      421 gaagacaaac gtatttccca gcgctaccag tttataacca tgcgcctgat tatcttgttc
      481 aagaaagggt cgatgctgga agacattcgc atcaagttcg ccatgatttg tcgcatcgtt
      541 cggtagcaac gaaccgctaa atcctaccag ttcaacatcg aggccatatt tctcttttgc
      601 caccttttta gcgacttccg cgacatcttg ttctgcgcca tttataacgc caactttaat
      661 gtgttttgca tcgctgctac tctggtcgca acctgccagc agaattccgg ccagcaataa
      721 tgcggcccct gtccgtagat gatgtgttgt cagtttcatt tttttatcct tttattaatg
      781 attacctgac tatagcggta aggtcgctgc ggtttaaaaa acgaaacgct atcgataaga
      841 ataaaaagga ataaaagtgg aatataaagt ctggcacttc ttactgacca cgcaagcgcg
      901 tttcgtacag catgatgaga gcgatgaaag caaactacac ttatgcttca ttcgatacac
      961 atttgtaaaa ggttgaatcc atgaaaccaa cgacactact gcttattttc acttttttcg
     1021 ctatgccagg catcgtatac gccgagtcgc cttttagctc actgcagtcc gcaaaagaga
     1081 aaaccacggt attacaagat ttacgtaaaa tttgcacgcc acaggcgtca ttatcagatg
     1141 aagcgtggga aaagttaatg ttgtcagatg aaaacaataa acaacatata cgcgaggcca
     1201 tcgtggcgat ggaacgtaat aatcagagca attattggga agcgttgggt aaggtagaat
     1261 gcccggatat gtaagaacag caacaactac tgaccaacgc ttctttacct gatatcagga
     1321 tttcttcatt ttcacctttg cagtaaccaa caatgcagtc agcaacatca atgtgccaga
     1381 aaacatcaac ggcgaagtca gaccaatatt atccagcgca taaccgccga ttgccgcgcc
     1441 acaagctgaa taaccgccac ctgaatagac ccggcttttt ctgcctgatc ggccagtgag
     1501 cgggtgatcc acgttgacca gccgacggga accaatgcaa aagttagccc ccagataatc
     1561 gccacgccgg tagcaacgat tttatcgctt ccccacaacg tcagcaccaa cgcactcaca
     1621 gccagtatta acggcgcgcc tgctaaggcc agttttaccg aacgtttaag aatgaatgac
     1681 gaaagcgacg taccgataaa gctggcaatg ccaaaactca acagcaccag cgttaagcca
     1741 tccacgctga atcccgccag gttcatatac accgggcgaa tatatgtgaa gaaagcaaac
     1801 tgcccggcga aagacatgaa gatggcgatc atccctgcca tcacacccgg acgttgtaat
     1861 aagcggaaag tattttgttt ctgatgcgag ggttcgcctg gcagtgaagg caatgatttg
     1921 ataatccaga aaatacacag cacgcccatc accgccgccg cattaaagac attgcgccaa
     1981 ccgataagct cgcctaaaaa actgcccaac ggcgcggcaa tcaccagcgc aatagaaacc
     2041 gcgccgaaga tcaccgacag cgccttcggc accgtacgcg gcggcaccag acgcatggtc
     2101 agcgacgccg acatcgccca gaacccgccc agcgccagcc ccagacaggc acgaccgatt
     2161 aaaagcaaac tgaatgagtt agcaaaggaa accagcaagc aggagagcgt cagcaaaacg
     2221 gcaaacaaaa taacaacgtt gcggcggtca gtagcctgaa ttgtctgggt aataaacaaa
     2281 ctggcaaaca ttgccacaaa ggcggtcacg gtcaccgatt gcccggcaac cccttccgaa
     2341 atgcctaaat cctgggccat tggcgtcaac aaactgacgg gcaaaaactc aacgataatc
     2401 agacaggcga cacaaaacgc caccgagaaa acggctgacc agttcggtcg ggtgatggca
     2461 tccgcgccgc ggttttcggc aataaattca ctcatggtgt tacccgtggc atgtttcagg
     2521 ggaaaagccg accactttaa cattgaaatg tgtgacgcat ttaacgtttt tgcaactttt
     2581 acggtgttgc ttgagctgga gttaagatcg aaacggaaca gggcaaagat taccggatgc
     2641 ggcgtgaacg tcttatccac ccgacaggtc aggcgcggtc tgtaggctga taagacgaag
     2701 cggttttaat gcagccagtg aacgccctcg gcaggctgaa agaacgcgtt atagcgcatc
     2761 tgaaaagcat taatatcctg catttcaggt tcggtttcgc gcagaaaacc cagcgccagc
     2821 ctgacctgag catcggtaat cggcgcggaa aggcgggctt tttgtaaaat ccgatgccat
     2881 tgcagcaggt tctgttcgct accatcgaca atgacaattt gccagatgag caacacctgc
     2941 gcggcgtttt gcaggtgcga ctcatccggg cgatgcaaat aacgtaaaaa ttcgtcaccg
     3001 gctgactttc ccatttgatc gaccatcgac tccacgggta cgacatcgat attcagccga
     3061 tcactgagac ggcgaatcgc ttttctggcc ccggatgtca gcacccgaaa accaaccaca
     3121 aacactaccg ccagcgtggc gagcattatc cagatcatga gttctcctgc gttatcagaa
     3181 ctcatgatat ctggaaaccc tcgccagtta ctaatccacc agaataatct tcagtgcaaa
     3241 cagagatgcg accaccacca cacacaggtt cagatcgcgc cagcgcccgg tgcacacttt
     3301 catgatgcag tacgacataa agccgagtgc aatcccttcg gtgatcgaga aagtaaaggg
     3361 catcatcacc gtggtaataa acgcaggcac cgattcggta aaatcatccc agttaacgcg
     3421 cgccaggctc gaagtcatca gcacgccaac aaagattaac gctccggcgg ttgcgtaagg
     3481 aggaaccatc gccaccagcg gggagaagaa cataaccaac aggaacataa cgccaaccac
     3541 aaccgcagtc agccccgtgc ggccaccgac tgccacacca gaagtacttt caatataggc
     3601 agtaacagac gaggtgccga taaacgcacc cgccaccgaa ctgacgctat caacatacag
     3661 cgccttattc atattgggga atttaccgtt accatctatt aagcccgctt tatcagttac
     3721 accaattaat gttcctgatg aatcaaatag gttgatcagc ataaaggaga aaatgatacc
     3781 ggcgagttca agtgttaacg cgccgctcaa atctacttca ccaatgacgc cgctaatatc
     3841 aggcggaatg gaatagacgc cgctaaaatg aacatcaccg aaaaataatc cacagcaaga
     3901 cgtcaccaca atagaaacca gcaccgcggc atgaaaatga cgtgatgaca acacggttat
     3961 aataaaaaaa cctaaaatac ctaacaacac gccgtgagaa cttaaatcgc caatcatcac
     4021 cagcgtgtct ttattggcga caataacgcc agtatttttt aatcccatta aggcgataaa
     4081 taatccaatt ccgggtaatg actccaa
//
LOCUS       DAETQN010000236         4082 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.239, whole genome shotgun sequence.
ACCESSION   DAETQN010000236 DAETQN010000000
VERSION     DAETQN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.239"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(46..747)
                     /locus_tag="K0W68_004346"
     CDS             complement(46..747)
                     /locus_tag="K0W68_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBI3075711.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPK
                     LQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYAKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTG
                     ERIIALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
     gene            complement(1280..1534)
                     /locus_tag="K0W68_004347"
     CDS             complement(1280..1534)
                     /locus_tag="K0W68_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HBI3075712.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
     gene            1713..3872
                     /locus_tag="K0W68_004348"
     CDS             1713..3872
                     /locus_tag="K0W68_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBI3075713.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEAL
                     PKDSNAAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGL
                     AALRVQLLKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAP
                     MPGNGEMTCYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHP
                     PLQRVYSSATFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVD
                     FTQGRLLPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNS
                     QLVISALTDKGLRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQP
                     DHLFRSLGFNRTWDEWRREEDARTHATERRHDRGWSQ"
BASE COUNT         1074 a         1032 c          986 g          990 t
ORIGIN      
        1 aaaaaaaacg cagcagggag cgttttaggt gccttaaaat cgattttaga tcgtcatctt
       61 gatttgtttg tcttccttga agcgaaaatg cagggctaca accgtgcgcc cctttttgat
      121 cgtctcaacc gttacagcca ggtcgctttt tttgttcagt tcgtcaatgc agggtcttaa
      181 taattgctgg ttaagtgatt tatatgtttg atatttatgc tctatatcaa gcattgagcg
      241 aaaatcatcg agagcgataa tacgttcgcc agtactgcgg aactgctgga ggagttcgta
      301 tatccttata ctgtaacttc ttgataatct agatatattc ttgattacaa ctctagtaaa
      361 ttgcccttgt agttgtgtca aataaggcat tactgcgtca gaaaatgtta tttgtgctct
      421 ggcctcgcct ttagcgtact gtgcccgata ctggatccag cggaactcct cacgtttctc
      481 gtcatccttg agaatgattg acctgtccca caaacgatca attgcttcct gtaactgaac
      541 ctcggcattt ttccgcccca tgtcagggaa tgaatcgaaa tattccgcag ctgtaatggt
      601 cattgtcttt tgcaattttg gagattctgc atcagcccca gacttcaatc gaccgatgca
      661 aagtaacaaa aatcgctgtt cctgtagggt caatttataa ctggcttcaa ccagcgcatt
      721 ggatttatag gcaattagat ccgacataga gcatccctcc ccaaaaggta atgaataaaa
      781 gataaacata ccctcaacga aaggcaatgc ctttttattt taatatccct ttttctgtga
      841 atattacctt aaatcatatt gcctttacct taaattcccc cacctaaaca ccttaaatca
      901 tattaccttt accttaaatc acattaccta tagcattgta acattttgtt ttctataagg
      961 aattttggcg ctaaaacaag tattaaaaac aagatcttaa aaacaagatc taaaaactta
     1021 acgacgagcc tgtggataac ttggagtgct gaaaacaaaa aaccaataaa aacaatgcgt
     1081 tgcaaaaaag cagaattgca agttccgaat acaccccctc caaccctgtg gaaatccagt
     1141 attcatgcgg gttcagcgca tatcacctcc cgaaaaaccg tgcgtatgtg ctcaatcagg
     1201 cgacggattc acttccgctt cctcgcccac tcgctcgcta acgctcggtc gctgggactg
     1261 cggcgagcgg acgatacaat cactgtcctg gcagcgcttc cagctcgtta atcagcggca
     1321 gtatgcgcgt cacatcagcc tgtcgtgtca cctctgcctt caggaactgg agtaaccagg
     1381 cacgcaactg cacctcctcc ggctttctga cctttgccag caatgctgca cccacaatga
     1441 ccttctgcgc tgtgtccaga ctcctgctct tcgccttcag gcgctgtaat ctggcctcag
     1501 catcagcaat cttctgttcg agtgttctgc tcatgatgta actccgtgcg cgatgaaaaa
     1561 tcgcatttta gcgcgtcact ggtagtttaa aaactgagct ggcataatgc acggaaaatc
     1621 acgaagtgcg cacttataca atctccactc cgtttcgatt gtgtgcgccc tgcggggctg
     1681 aaagaaaacc acaaaaaggc attacggcag aaatggcgat ttatcatctc agcatgaaaa
     1741 tcatctccag aaacagcggc tacagcgccg ttgcttctgc tgcctatcgt tccggttcac
     1801 tgatgcttga tgagcgtacc ggactgactc acgactacac ccgcaaaagc ggtgtggctg
     1861 aagctgtgat tctgactccc gcgacagccc ctgcctggtg tacgaaccgt gccgaactct
     1921 ggaatgccgt tgagaaggcc gaacgccgga agaactcaca acttgcgcgg gaaattgaac
     1981 tcgccatccc ccgcgaacta ccgcaggatg ccgcacggga aaccgttctc gcctttgtca
     2041 gagaaaactt tgtcagtcag ggcatgattg ctgatgtggc gttccatcac atggataaaa
     2101 caaatcccca tgcgcacatc atgctgacca cgagagctgt cggacctgct ggattcggtg
     2161 gaaaggtcag ggactggaac gaccggacac atgccgagac gtggcgtgca tcatgggctg
     2221 accatgcgaa cagggcactg gcgaacgccg gttatcagga agagatagac catcgttcat
     2281 acgagcgaca ggggcttgag aaaacgcctg gtattcacct cggaaagtct gcctgtgcga
     2341 tggaaacacg cggtatcgaa acagaacgcg gcgaacagaa tcgtctgatt aacagactta
     2401 acctggaaat acagatttcc cgtaccgaac tcagaaacag cgggctgaca gagcctgccc
     2461 gccgtgtggc tgaccttctg aatatcgtgc ttcctgacaa cgcaacttcg tacacactac
     2521 gtgacatcat cgaagcgttg ccgaaggaca gtaatgcggc atggaaactc accagtaatg
     2581 ccatgtcgat gatggcagat atgcaggcga cgcgcagacg ctgggaagag ctgaacaccg
     2641 aacgaaagga agcgatgagc catgctgcca gcctgaaaaa atcatcgccg ttttcgtccg
     2701 gtttttcccg tattcccctg atgcaatggg cggcaccgga ataccgtagc gaacagggaa
     2761 aaatccagtc gctgagccag caaatggaaa aattgcgaca gcattaccgg aaggtggaga
     2821 aacaggacat tcccgcgagt cagacggcat ttgaaaaaca gtggcagcag tggggggcat
     2881 ccggccttgc agctctcaga gtccagttgc ttaaacgtga agaggaattg cgccggaagg
     2941 agcaggagga tacagaacgt agtcgcgcgg agcgacgagc ggaacagctc aggaaaaatg
     3001 acaatgccgt tatggattca ggggtactga gcgcagtcgt tacggggtat ggcgaggccc
     3061 ccatgcctgg taacggggaa atgacctgtt accttctgct tcacaaccgg aacggagaat
     3121 acacgctctg ggggaacgag ctggagaaat accgcaccag aatctttgag cgtgttgatt
     3181 taatgcgtga caggagtggt tatatctgtg accgaagcga aatcggtcaa catccaccac
     3241 tgcaacgtgt ttattcctct gcgacctttg agcaacttct gacgcaggtt tgccagacat
     3301 ggccgcaata tacccgcaat ctccgccagc cgaagacatg gccggagtct ttctgtcttg
     3361 gagaagaccg ccagccagcc atgccatcac tggcagcaag gaaggtggat tttacgcagg
     3421 gacgtctgct accgacgctg atgccggtaa tgagttctgt cgaccgggaa acccgccagc
     3481 ttcagctttt gctggtcatg ggcgtggatg actctctggg cggtgttgtc cggcttaatg
     3541 gtacgctgta tcccgcgttt gccgttccct cagccgataa cagccagctg gtcatcagtg
     3601 cactgaccga taaggggtta cggtatgcag ggtatggtgt tgccgtaaat cacgatgcag
     3661 acagccacat atcacctgcg ccggaactta tggagtttca tctgaaaacc cgtgaggcac
     3721 cgctttttgc tgccgtcaac acaccagaaa aacagccgga tcatctgttc cgctcgctgg
     3781 gttttaatcg tacctgggac gaatggcggc gcgaggaaga tgcacgaaca cacgccaccg
     3841 agaggagaca tgatcgcggt tggtcacagt gaatgtgaac aatattttgt tttgtttaac
     3901 atcgtgttat gttcgtcgat gttcttcgat gttcatcgac agggatgaat gcaagaagca
     3961 cacacataat gcaagcatag ttttgttttt ccgcccccaa aaaatggggg ttcttttttc
     4021 gctgcattag agcgcttttg acgcaatttt tcacttcaat agtaccttcg cactactaac
     4081 ac
//
LOCUS       DAETQN010000237         4079 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.240, whole genome shotgun sequence.
ACCESSION   DAETQN010000237 DAETQN010000000
VERSION     DAETQN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.240"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..787
                     /locus_tag="K0W68_004349"
     CDS             <1..787
                     /locus_tag="K0W68_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBI3075714.1"
                     /translation="IAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYV
                     NQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQL
                     SDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQ
                     GNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQG
                     SRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            800..1760
                     /locus_tag="K0W68_004350"
                     /pseudo
     CDS             800..1760
                     /locus_tag="K0W68_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(1798..2697)
                     /gene="nhaR"
                     /locus_tag="K0W68_004351"
     CDS             complement(1798..2697)
                     /gene="nhaR"
                     /locus_tag="K0W68_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBI3075715.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2763..3929)
                     /gene="nhaA"
                     /locus_tag="K0W68_004352"
     CDS             complement(2763..3929)
                     /gene="nhaA"
                     /locus_tag="K0W68_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBI3075716.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1226 a          953 c          861 g         1039 t
ORIGIN      
        1 tattgccaaa acacgtcacc aaattaactt atcgaattcg acctcatttt ccaaagatgg
       61 ctattcctcc aacaatactg gaattactgg catagccggt gaacatgatc agttaaatta
      121 tggtatatat gttaatcagc aacaacaaaa taatgatacc tcgcttggta cgaatttaag
      181 ctggagaact cccatcgcca taatagatgg cagctatagt cattctaaaa acgcctggca
      241 aagtggtgga agtattagta gtggattagt tgtctggccc ggtggtatta atatcactaa
      301 ccagttatcc gatacatttg caattctgga tgcgcctggg ttagaaggtg cacatattaa
      361 tggacaaaaa tacaatcgaa caaacagcaa aggccaggtt gtttacgacc tgataatacc
      421 tcatcgtgaa aaccatctgg tacttgatat agcaaacagt gaaagtgaaa cagaattgca
      481 gggcaatcgt caaattattg caccttaccg tggagcagtt tcttatgtgc agtttacaac
      541 tgaccaacgt aagccctggt atatacaggc actgcgtccc gatggttcgc cattaacctt
      601 tggctatgac gtactggatc tccaggaaaa taatattgga gtcgttggcc agggtagtcg
      661 cctttttatt cgcgtagatg aaattcccac tggaataaaa gttgctctca atgatgaaca
      721 gaacttattc tgtactatta cttttcaaca cgttatcgat gaaaacaaaa catatatatg
      781 ccagtaaaag gagtttacta tgaagtggct tttattaatc accttgagtc tatattcttt
      841 tattgtgcaa agtgctccct gtgcattgac caatgtcggg gaacaacgtg gaacatatat
      901 actcaagcca ctgagtatga aaggtaatct caccgccgaa aaaattgaaa acaccggaaa
      961 tatcattgag tgtgcagact tacaagagac gaccaacgcc attaaagttc attttttaac
     1021 acagaacgct ttaaggttta aagtcggaag tcgtcattat attattaatt tcccctttga
     1081 atctgacggt aaaaaaaata gagcttccag gaaggtcatc ttataattta gaagaaatgc
     1141 tatctgcgat aaatttcacc attccatact ctatcgcatc tgtatcagat agttccaaca
     1201 ccgcagatat ttttccagga actccttttc ctttgttgtt cgaaattgaa gtgagttcat
     1261 gtacagggaa taatactaat tgtaatagcg tccgctttaa ttataattta aatactattt
     1321 tacaggtaaa tttaatgacc tgtggttttg aagatcaatc catcgacact ggacgatttg
     1381 tactgtctcg tatcaataat gaaaattatc agtttcatca tataagattt gactgcatcc
     1441 agggcgagca aggtgaatta gtttttgaac ccagtgatgt tgaatattac tttgaaccag
     1501 tgacgataca ggagtcagga accagcatat taaaaaatga gttagaaaac agtgaggacg
     1561 gtgcgggtca agtaggcttt caaccctcca atgatggtac gaatgaaatc cagtatggta
     1621 aaagcaatta ctactctttt cagcatcccc atgaaggaag taatcagatt cctctattta
     1681 ttcgcccgcg tacatatgga aataatgtct caagcgggca aataatgagc cgagtcaaaa
     1741 tcgtggttat gtacaactaa tctatctccc ccaccttaac tttgggggag gtgccgatta
     1801 acgcgccgct ggactaaaaa gcgcagaata atccgtattg cagattcgct gtaccgccgg
     1861 gtgctgaatc atccgctcag caaaaatagc atggtactct tccatcacat tctcgacgcg
     1921 accaatttct acgacagttt tatcggcata aaagtcatat gcataaagcg ttggggcaac
     1981 gaagattgca ttgtgcatcg caccaaaagc tttcatcaac gcggcatcat caaactcgcc
     2041 gaggatttct acgtttaatc cctgggagtt aaaccagtta agcaatttgc gccctaacat
     2101 tgaacgtcgc ccaggaatca aaagtcgccg ttcttccaga caagccggga acgatttttc
     2161 tggtggtgga tttgtacacc agaaactcac gccacattcg ccaattctca cggagaacag
     2221 gccttcctgc tgcgtagagt ctatcggaca gtcagaaatg atcatatcca gtttatgctg
     2281 acttaattgc tccagcagca tttcgtgggt ggattcgaag cagcgaagat gaatgggctc
     2341 gccttctacc actgcggcgt taagtacgct actgaccagg cgtttggaaa gtgcatcagc
     2401 cacgccaacg tcaaacaata aattggattc tttgcgatag ttcacaatat ccagcatttc
     2461 ctggcttaag gtgaacattt tatcggcata gcgatagacc agttctccca gctcgctggg
     2521 ttcgagacca cgtcccttgc gtttaaataa tttgccttgc aggcgctctt ccagcgctcg
     2581 aatctgtccg gtaatggttt gtggagttaa ataaagcgcc tccgctgcgc caaccacgga
     2641 accttcttta tagacatgcc agaagtaata caagtggttg taattgatat gagacatgct
     2701 catttctctc cctgataaca atgaaaaggg agccgtttat ggctccccgg taaaccgtcc
     2761 tgtcaaactg atggacgcaa acgaacgcgt aaccagctgt atccaattac cgccgaagag
     2821 atagaaccga ccaggatacc gagtttcgcc cagttaatca gttctggatc tacgctacca
     2881 aaggccaggc tggcaataaa gatagacata gtaaaaccga taccgcacag gatccccacc
     2941 gccataattt gctgataagt cgttccctca ggcagatgcg ccagtttcaa acgcagcgcc
     3001 aaccagcaga acagactaat ccccagcggt ttgccaatca gcaagccagc gatgatcccc
     3061 aatggcagaa tggaggtcaa gccatccagc gtgacgcctt gcagtgaaac gccagcatta
     3121 gcaaatgcaa acagcggtaa aatcagatac gccacccacg ggtgcaatac atgctccagt
     3181 cgcttcgctg gagaacgccc atgcttctct ttcaaaggaa taaagaagcc gacaattacc
     3241 cccgccaggg ttgcgtgaac ccccgatttc aacaccgcag tccacaacac cacgccaaca
     3301 agaatataga cgcccgtgcg gcgtacacca cacagattca ataccgcgag taccgcaatt
     3361 gctacagccg cgacgccaag agaggccatc gataagtcat tagtgtagaa caatgcgatg
     3421 ataatgatgg ccccaagatc gtcgataata gccagagcca tcaaaaagat cttcagtgct
     3481 aacggaacac gacttcccaa cagcgccagc acaccaagtg caaaggcaat gtcagtagcc
     3541 gccgggatcg cccacccttc gcgggtaatc ggatcggcat agttaaaggc cagatagagt
     3601 aatgccggaa caatcatccc accaatagcg gcgataaccg gaaatgcggc ctggcgtaag
     3661 ctggctagcg atccttgcat cagttcacgt ttaacttcca gaccgactaa caggaaaaat
     3721 accgccatca gcgcgtcatt tatccataac agcatgtttt tgttgatttc gagtgaacca
     3781 acccggagct gaaccggcgt ctccagaaag tcgtgatacc atccactggt tgcgccgctg
     3841 ttggccatca tcatcgccag gatagcggca atgataagaa taatgcctcc cgaggcatca
     3901 ctgctaaaga atcgatgcag atgtttcact ttttatttct ctttcaggtg aatagatcgt
     3961 ttttactatt ttaccccgca caaatcatcg ttaaaatcag attatagtgt aggaataact
     4021 cgttttttac gagcaattaa aggatttgcg ccatcagcgg tgagatgagt ttttgacgg
//
LOCUS       DAETQN010000238         4045 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.241, whole genome shotgun sequence.
ACCESSION   DAETQN010000238 DAETQN010000000
VERSION     DAETQN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.241"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="K0W68_004353"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="K0W68_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBI3075717.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="K0W68_004354"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="K0W68_004354"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBI3075718.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="K0W68_004355"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="K0W68_004355"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBI3075719.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="K0W68_004356"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="K0W68_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBI3075720.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          969 a         1142 c          970 g          964 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat catcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caccccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacc
//
LOCUS       DAETQN010000239         4003 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.243, whole genome shotgun sequence.
ACCESSION   DAETQN010000239 DAETQN010000000
VERSION     DAETQN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.243"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            168..503
                     /locus_tag="K0W68_004357"
     CDS             168..503
                     /locus_tag="K0W68_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3075721.1"
                     /translation="MLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTED
                     TIDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLT
                     LSYREVNRD"
     gene            496..978
                     /locus_tag="K0W68_004358"
     CDS             496..978
                     /locus_tag="K0W68_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075722.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1012..1092)
                     /locus_tag="K0W68_004359"
     CDS             complement(1012..1092)
                     /locus_tag="K0W68_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075723.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1128..1552)
                     /locus_tag="K0W68_004360"
                     /pseudo
     CDS             complement(1128..1552)
                     /locus_tag="K0W68_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1982..2472
                     /locus_tag="K0W68_004361"
                     /pseudo
     CDS             1982..2472
                     /locus_tag="K0W68_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2697..3188
                     /locus_tag="K0W68_004362"
     CDS             2697..3188
                     /locus_tag="K0W68_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBI3075724.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            3523..3981
                     /locus_tag="K0W68_004363"
     CDS             3523..3981
                     /locus_tag="K0W68_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBI3075725.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDG"
BASE COUNT         1495 a          644 c          646 g         1218 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 ttatggcatg caataaaaac tataaccaac atgaagtggc agactgttta accctgtctt
      481 accgtgaggt taatcgtgat tgattataaa aaaaacttat tatttatttt agtttttatc
      541 agtgggttta ttctcttcac tgtctattct tacactgcag aaaaaatgat atataacgaa
      601 acatgcactg caaattgggt aatttttaat gaccagggac gagcaaatct taccatcgac
      661 tttatgtata acaaaaaaaa taaaacagga actgttgcgt tgagtggaac ctggcaacaa
      721 ggtaacagag aaagtaaatc aatacggcga aatattgaat acacatggat tgaaaactat
      781 gacacagccc atttaacatc aaagaaagtt aataagtttg agattatgga tcaagttgat
      841 gatgatagac ttgcacaact tattcctgat ttttatgtct ttccagaaaa aagtgtaagc
      901 tataatatac taaagcaagg taagcatgct tttattttga gcattggtaa cagagcaata
      961 atgcattgtg caaggtaaac taaaatagat aacaaaaaag atggaacctc gttaatcata
     1021 aaacaataaa attaaatatt ctcgcagtat atggcagtct aaagcatcaa agttttgatc
     1081 aacatctttc attttagaca tctccttgca atgtaaaata tcatgtatca acaacatctg
     1141 gtgcaaatcc atgagtcgaa ctcgacattc atcttagctc gattattatt ccttcgtaca
     1201 gtcgatgtaa acaatacaga aagaggatta ttaagaacca gtttactcag gaatcaatag
     1261 aggaaaggac taacgtttct ttaaaagaat tgatttcatc atctgttaaa ctaaactcat
     1321 cattgacaga tcgtgagata taactgtttt taactttact ctttacgttg actttattga
     1381 cagaattaac attcacatat cttgaattta atgtccatga tgttgtttgc gtgagaacat
     1441 tctcagcatt aaggaatttt tttaacggaa tccttggttt ctttttcgag tccgaattta
     1501 caatatcatg catatagacc atttcatgaa tctgcgaaaa ctcaatgtcc attgtatacc
     1561 tcacattttt accgtgactc gatgttactg ttcaataatc accttccatc aatactaaaa
     1621 ttaatacccc taatgtgccg ataacaaata tagtcattct acgtaacgtc tccataaggt
     1681 gataaccttg atcaaatgaa tttcgaatat aataaaggaa atcttttgag atgtttatca
     1741 cgaataaagc tatttatgtt accaatatca tttcgagtgc gcacactatc tatctgtttt
     1801 ttcgagatat aaaaaacaac gtgtaagtta tgtaaattgc cgatgctact gagtaacatt
     1861 atggtaaaaa aagtaaaaat gagcgatttt tgatagaata aagcatgaga aaaggtgata
     1921 tttgacatta tcagaagctg cgaattcgga ttttgctcta atcaaatgag agaaatgtaa
     1981 aatgaagcca cgaaatatta ataatagcct accactgcaa ccattagttc ctgatcagga
     2041 gaacaaaaat aagaaaaatg aagagaaatc cgttaatcca gttaaaatca caatggggtc
     2101 tggtttaaat tatattgaac aagaatctct tggaggaaaa tatctaacac atgatttgtc
     2161 aataaagata gcggatattt ctgaagagat aattcagcaa gcaatattat ctgctatgag
     2221 catatataaa ttttcgataa cagatgattt aatgagtatg gctgtaaatg aactcataaa
     2281 actgaccaaa atagagaata atgtagacct gaataaattc actactatat gcacagacgt
     2341 tctatccccc ctcgtcacca gacataataa agaaaaaaaa caaacgacat tctacccttc
     2401 tcaaaaatcc cctttttaat tttcattgaa aaaatggcaa ttgatactat gaatccaggt
     2461 catacacatt aaggcattta tgtaatgtcg taattaagat aactaataag gtgaatatta
     2521 gtaaaacaac taatgatgtt attagtttgt agtgaacagt acttttacca ataatgaaaa
     2581 atataccata agcaatgcaa gcttaaaact aataatttaa aaaagataaa tgtaaaaata
     2641 ttaatgagtc aatcaataat aatttagcat cacgagacac atcacagagg aatattatga
     2701 gcacagaaac aattgaaata ttcaataata gtgatgaatg ggcaaatcaa ctaaaacacg
     2761 cattatcgaa aggagaaaat ctggcattac tacatggttt aactcctgat atccttgata
     2821 gaatatatgc atatgcattc gactaccatg aaaagggtaa tataacggac gcagaaattt
     2881 attataaatt tttgtgcatt tatgcgttcg aaaatcatga gtatctaaaa gattttgcat
     2941 cagtatgtca gccaaaaaag aaatatcaac aagcatatga cctttacaaa ctaagttaca
     3001 attactcccc gtatgatgac tattcagtta tttatcgtat gggtcaatgt cagattgggg
     3061 ctaaaaatat cgataacgca atgcaatgtt tctatcacat tattaacaat tgtgaggatg
     3121 atagtgttaa gagtaaagcg caggcatata ttgaactctt aaacgataat tcagaagata
     3181 atggctaagc attacaatca tttggttaaa tttgctggtt atagtgcagt taactactat
     3241 aaccagcacc tcttatgttt tcttaaatca gcatcttaaa acagttaaga gcatactata
     3301 aatcattatt tttcaatgcg ttagagaaca atctacttta taaaaattga ataaggctat
     3361 ttcagattac atcactgaat tcctaagata aattagtgaa cacaaccata gatatgtcga
     3421 atgtattttc ctgtagcatt aatgacacat aaatatgaat agccataatt tctattgctt
     3481 aagcaggatg caagaaacca attttttcat agaggttaac taatggactt agaaaataaa
     3541 ttctcatatc attttcttga gggattaacg ctcacggaag atggaattct tactcaagga
     3601 aatgagcaag tttatattcc acagaaagag ttaggtgtat taatagtatt acttgaatcc
     3661 gctggtcatg ttgtactgaa agatatgatc atcgaatcag tatggaaaaa tattattgtt
     3721 agtgacgagt ccctgacaag atgtatctat tctttgcgct gcatttttga aaaaattggc
     3781 tatgatcgtt gcatagaaac aatctaccgg aaaggttatc gtttcagcgg gcaggttttc
     3841 aaaactaaaa ttaatgaaga taatacttca gactattcca tagctatatt ccctttcact
     3901 acttcattga atacactgga tccattaata cttaatcagg aattagtgca aatcatttca
     3961 aataaaaaaa tcgatggtta gactggcccc ctgaatctcc aga
//
LOCUS       DAETQN010000240         3959 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.244, whole genome shotgun sequence.
ACCESSION   DAETQN010000240 DAETQN010000000
VERSION     DAETQN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.244"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..242)
                     /locus_tag="K0W68_004364"
     CDS             complement(<1..242)
                     /locus_tag="K0W68_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBI3075726.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="K0W68_004365"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="K0W68_004365"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBI3075727.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="K0W68_004366"
     CDS             2255..3904
                     /locus_tag="K0W68_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBI3075728.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1031 a         1001 c          927 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagg
//
LOCUS       DAETQN010000241         3950 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.245, whole genome shotgun sequence.
ACCESSION   DAETQN010000241 DAETQN010000000
VERSION     DAETQN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.245"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            753..1181
                     /gene="uspC"
                     /locus_tag="K0W68_004367"
     CDS             753..1181
                     /gene="uspC"
                     /locus_tag="K0W68_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HBI3075729.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1188..2612)
                     /gene="otsA"
                     /locus_tag="K0W68_004368"
     CDS             complement(1188..2612)
                     /gene="otsA"
                     /locus_tag="K0W68_004368"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HBI3075730.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(2587..3387)
                     /gene="otsB"
                     /locus_tag="K0W68_004369"
     CDS             complement(2587..3387)
                     /gene="otsB"
                     /locus_tag="K0W68_004369"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBI3075731.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(3554..>3950)
                     /gene="araH"
                     /locus_tag="K0W68_004370"
     CDS             complement(3554..>3950)
                     /gene="araH"
                     /locus_tag="K0W68_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBI3075732.1"
                     /translation="RNTLAIGGNEEAARLAGVPVVRTKIIIFVLSGLVSAIAGIILAS
                     RMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKISYVVAGILILGTVENAMNLLNIS
                     PFAQYVVRGLILLAAVIFDRYKQKAKRIV"
BASE COUNT         1064 a          948 c          885 g         1053 t
ORIGIN      
        1 tttttatgcg gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt
       61 agcattagaa tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt
      121 ccttacaaat agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact
      181 gtactcgtca ctacacgcac atacaacgga ggggggctgc gattttcaat aatgtgtgat
      241 gcagatcaca caaaacactc aattacttaa cataaatgta taagtcatac ttttgttttg
      301 ggtgtatttc aatatgttaa aaagtttttc gctacgctag caagcaaaaa tgaaacagga
      361 ataatcgaaa tgggatgttg cgcacagtta aaataaatca ccgtaaataa tcatctgcta
      421 taaataatca ctttcatgca ataccagata aactattttt aaacagacac ttaccacaca
      481 acaaactaat taaatacaaa gaaaggcaac aatcaactta atgttaatga aatgttttac
      541 atacattaac cacttgttta atgcggagaa tcagtacact attttcttat aattacattt
      601 gaaattatat gatcaccggg agtgattgat taattgtctt tactaataat cagactaata
      661 tttacctgtt tgaccgagtt gggattgcgt cgtttctcca ttaggagtaa agctttaatg
      721 tcacctgaag ttcacagaat aaggaacagg ttatgagcta tagcaatatt cttgtcgctg
      781 ttgcggttac accggaaagt cagcaactgc tggcaaaagc ggtatctatc gccaggccag
      841 taaagggaca catcagttta attactctcg cttccgaccc ggaaatgtac aatcaattag
      901 ccgcgccgat gcttgaagat ttacgcagcg tgatgcaaga agaaacgcaa agctttcttg
      961 ataagttcat tcaggatgcg gggtatcccg ttgacaagac gtttattgcc tacggtgagt
     1021 taagcgaaca tattctggaa gtatgtcaca aacatcattt cgatttggtg atttgcggta
     1081 atcacaatca cagtttcttt tcgcgagcat cctgctcggc gaaaagagtg attgcctcaa
     1141 gtgaggtcga tgtgctgtta gttccactta cgggagatta accgctccta cgcaagcttt
     1201 ggaaaggtag caactttatc gcgctgctgg ctttccgcgc ttcgcggaac tatctgcttt
     1261 aggtcgctaa tgaagcactc ctgccagtgg ttaatatcgt ttttcacgat aacgtccagc
     1321 atttctgcat gacgggaaat acgttccgcc agcgacatag tcaatgcacg atccagcgca
     1381 gctgcaactt cgtcacgatc gtaggggtta acaattaacg ccgacgttaa ctcgtttgcc
     1441 gctcccgcaa attgcgaaag aacaagaacg cccgggttgg ctgggtcctg agcagcaaca
     1501 tactcttttg ctaccaggtt catcccgtca cgcagtggcg tcactaagcc cacgtcagag
     1561 tagcggaata ttttcatcag taatttacgg tcaaaatgct gattcaaata ataaagcggc
     1621 gtccagccta attgcccgta tttaccatta attcgtccag cttcattttc gagctgatga
     1681 cgaatatcct gataggcttg cacatcacca cgcgacgttg gtgcaatctg ggtataacga
     1741 attttaccat gatgctgcgg atatttttcc agcaacgctt cataggcgag aaaacgctct
     1801 ggcaaacctt tggaataatc cagccgttcg acagaaaaga tattttgtac gtttttcagt
     1861 tccgctttaa gttgcgccag ttttggcggc agtggcccgg cagcctgttt ggctatttct
     1921 ttcggttcaa tgccgatcgg gtagacttct gttcgaaatg ctttgcccca ggctgtatgg
     1981 ctttttgcgc tacgtgtcgt gacgcgggtc aggttagaaa gacaatccag gaacgccaga
     2041 cgatcgtttt ctgtctggaa acccagcaaa tcataatcac aaagctgttc aagcaaggtg
     2101 tcatatgtcg gcagcgcgtt gaagatttcc ggtgtcggga aaggaatatg cagaaagaaa
     2161 ccaatgcgat tattcactcc ccgtttgcgt aattcatgcg caaatggcaa caggtgataa
     2221 tcgtggatcc agataatgtc atcgtcttgc aacagcggca gtaatttatc tgccagcaac
     2281 gcatttacgc gtagatagcc gtcccaggca ggacgctgaa attgcaccag atcgagccga
     2341 taatgaaaag cgggccagag aacggcattg gagaattggt tgtagtattc gtcaaggtcc
     2401 tgttcgctga ggttaaaaga ggcccacgta atgttacctt ttttcacctt ttttagcggc
     2461 tgatcctcat tccctgtttc accactccag ccaaaccaca gtccgcctgc ggctttcagt
     2521 gcccccagta tgccaacggc aaggccaccg gcactggcgg cgtgctcgtc tggtggtgca
     2581 atccggttag atactacgac taaacgactc atagtcatca ctcctgttat tttctctttt
     2641 ttgttgtaat gcggtggtta tcatttcaag ccagctccag acatccggca cacccgccag
     2701 tcgccatgat gcctgagttg cacctgtgcc aatttttact gacattccgc ccagtcggtt
     2761 aacgactgcg aagccagatt catcggttaa atcatcgccc agaaatacgg gcgttcgccc
     2821 gataaaggga gcttcctgca taaaagctgc aattgcctca cctttactgg tacctctcgg
     2881 tttgatctcg acaacacact ttccctgctg taacgccatt tgtggccaga tctgagtaat
     2941 acgttgcgct aatgtcatta atgcgtcttc atgctgcgga gcctgacgat aatgcagcgc
     3001 aaaagccatc cctttcgcct ccagctccgc gccgggatac tgagcgatga ctgtatgcag
     3061 ttgcacgcta atatcacgcg caatcgcatc cggcagatga acgatatgtg ttttaccatt
     3121 gatgtcacgg cgctccgccc catgcacgcc cgctaacggg aagcgataag gttttgccag
     3181 tgcgtcaagc tccaccattg agcgccctga tatcaatgcc aatgcaccat cacttgcggt
     3241 tgccagtagc tgtagtcctt gcagaatatt gtcaggcacg acgacctgat cgggatgcgg
     3301 tttgatttcc gccagcgttc catcaagatc aaaaaaccag gcatatttcg cggatagttc
     3361 aggggtttcg gttaacggtt ctgtcacccg gctctcctcc ttctcttttc ttattgtttt
     3421 gcgcagaacg cgctttacag acatgtaagt atagacagtg tgacgggggt cgccatttgc
     3481 aatgaagaac agccaaccgt tgtggcggct ggctgtggtg ggaaaaaacg ttaaattgtt
     3541 gtggaaaaaa gcatcagaca atgcgtttcg ctttttgctt gtaacggtcg aagatcaccg
     3601 ctgccagcag gattaagccg cgaaccacgt actgcgcgaa aggagaaata ttaagcaggt
     3661 tcatggcgtt ttccacggtg cctaaaatta agatacccgc caccacatat gagatttttc
     3721 cgatgccacc tttcagagaa acgccaccta aaacgcaggc ggagataaca ataagctcat
     3781 aaccaatcga cgtcattggc tgcccactag tcatacgtga agccagaata attccggcta
     3841 tcgcagatac caggccagag agaacaaaga taataatttt ggtgcgaaca accggtacac
     3901 ccgccagacg cgcagcctct tcgttcccgc caatcgccag ggtgttacga
//
LOCUS       DAETQN010000242         3922 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.246, whole genome shotgun sequence.
ACCESSION   DAETQN010000242 DAETQN010000000
VERSION     DAETQN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.246"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..301
                     /locus_tag="K0W68_004371"
     CDS             <1..301
                     /locus_tag="K0W68_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075733.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            390..2258
                     /locus_tag="K0W68_004372"
     CDS             390..2258
                     /locus_tag="K0W68_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HBI3075734.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2295..3922
                     /locus_tag="K0W68_004373"
                     /pseudo
     CDS             2295..3922
                     /locus_tag="K0W68_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT         1231 a          721 c          769 g         1201 t
ORIGIN      
        1 acgtgtagaa ctgtattacc gctttaacca tgaagaagaa tattaccctt ctcaccgttt
       61 tatgcttgtt gttgatatga agacagtaat ggttttttca caacgagctg aatcactggg
      121 gttatactcg aataatcgct tgcttatcaa tataattgcc gaacatatcc atagtgatat
      181 atatctaacc aagtatcagc aacaatatcc agatgccaat gcattaataa atactttgca
      241 tgaatctggc aatgatatgg tgcaggatga aaaacatttg agtgatagca aaaaccgtta
      301 atattttcct ttcattgtga tcgtctgtag gcgatcacaa tctatttgtc ttttataacg
      361 tctaaacttt tttatgcaaa ggtgtattta tgattttttt taacgatata aagcccactg
      421 gttggttaaa gaataagatg gaatggtata tgaacggtca tataggagaa ctggataaat
      481 tagttcccca tcttattacg gagcataaaa tatatggaag agatcgtata actccacgca
      541 ctgtatctgc tgaagatggc gttattaaaa aacagaataa tcccgatgat aacatgatgc
      601 aatattattg gtgggatagt gaaactcaat cgaattggaa ggatggatat tgccgcagtg
      661 cctacttgtt agataataaa gaatggcagg aaaaagcgag cgatttatgt ttggaactta
      721 tcgatacgca agatgatgat ggctacattg gaatttatga ttcatcatta aaatttaact
      781 gtaaagcaga aaacggagag ttctgggcac aagctactgc cttgcgtttt gtgcttggat
      841 gttatgagtc cacccgtaat gagagtttat taacttctct tacccaagcc gcacaatgca
      901 ttatggctgg ctaccctaaa gaaacgtcac atcctttcgt tgtggaacag ggttttgctg
      961 gacatagcca cggattaaca ataacagatg ttttctaccg cctttatgaa ataaccaata
     1021 acattgaata tctctcatat tgcttgtggc tgtatgaaga ttattccgca ggatgcgtgt
     1081 cggagcaaga tctgcaatat aaaaatgtca tagaccctgg ttaccttctt aaaggtcatg
     1141 gcgtacatac gtatgagcat attagggcat tagccatcgc agcgagccaa cggccgcagt
     1201 atcaaactgc attagatatt tttttatccc agttgaaata ttatcttaca ccatctggcg
     1261 ctcccattgg tgatgagtgg attacaggac gcacagctaa tgccacttat acaggctatg
     1321 aatattgttc tgttcatgaa ctatttagca cttatgttat gttaataaaa ctgaccacta
     1381 atattggtct ggccgatgat gctgagtggc tattctataa tgctagtttt ggcatgcagc
     1441 atcccatact tcacgcgata atgtactgta agactgataa ttgttatgaa gcaaatgaaa
     1501 ggaaacatcc tgacagtaat cagtttaata ccagatataa atactcccct actcatgagg
     1561 aggctgctgt ctgctgcgta ccgaatacag caagaaccat accggctttt gtcgaaaata
     1621 tgtttcaaga aaataacaat ggttttactg cattatttta tggcccgtgt gagttttatg
     1681 gcacatttaa taatgttgca attaaaatta cacaattaac tgaataccct catgatttaa
     1741 gcgtaaaatg tctcattgag cctgagtcat ctgttcgttt tgctctcagt ttccgttacc
     1801 ccggctgggc aaaaatgatg attattaatg gtgttacttt tactaaaaat gatactcaaa
     1861 actcacttat tattttagat agaacatggc aacatcatga tgtgattgac attaaattta
     1921 tagctgatat acagtttaat actgacttat gtggtgacac ttatatttcc agaggtcccg
     1981 ttttatatgc aattgagctt gagagcgata ttttaatcaa aaaaaacctg ctgaacggga
     2041 aatattttga ctcaggatac ataccctgta gtagagctga tgaatctatc cagttctctt
     2101 atgctgatat ggaatctttt tcatattcaa cctcaccaga gggcaaacaa tacattgaag
     2161 gctgttttta tttaaacaag actaaaatag tcagaaaact tataccattt ggtcaaacca
     2221 ttcttcgcaa agtaactttc tccaatatag agagttaata ttttcgagtg ttataattaa
     2281 ttaaagggat ttctatgtac cttactgata atattatcaa actgtcgaat aaaataggtc
     2341 agcgtgttta tactgttgtc tctgaaatgt cgattgaagc atggataaca aaagaaccgc
     2401 agccttttgt ttatcgccgt agcggcgctt accagaaact caccattggc tcagactggg
     2461 gtcaactgtt tgactgtgcc tggatgcgag tttatggtaa acgtccagca gataaattta
     2521 gacacaaact cgtcgctctc gttgatatcg gtggcgaagg tcttattgtg gataagaatg
     2581 gttcacccat ctgcggcatc acgaataaag catcatctta tggcgtaccg ccagataaac
     2641 caggtaaatg ggtggttgac ctttctctcg ttagtgaaaa tgacgaggtt gagttttgga
     2701 ttgatgctgc ctgcaacgat ttatttggct atgttactaa tggagggatt attactgatg
     2761 ttcatatcgc gacacgtaat caactactga aatcccttta ttatgatatt gaggtgcttt
     2821 tcgactggat taatgatggt cagaagtttg aatccataca tcctaaaggt atcaccccag
     2881 aacaatttat tgccaaaaga agtgagcgta ctgacgaaat cataagtact ctcgaatata
     2941 ttgataatac gttaattacc tttagcaatg aagaaataat taaatgtcaa attgcaatcc
     3001 aatccataat caataaaaac aataatcctt cagagttcag gattatggct actggtcatg
     3061 cccatttaga tatagcgtgg atgtggcctt taagagaagg acgcagaaaa gcaataagaa
     3121 catttgccac cgcccttgca aacattgaaa aatatcctga ttacattttt ggcgctagtc
     3181 agtaccaact ttttcactgg ataaaaaaag attatcccta tttcttcgaa aagttaaaaa
     3241 aacaaatcgc cgcaggccgc tttgagttac aaggttgctt ctgggtcgaa tgcgatttaa
     3301 atctggtaag tggtgaatcg ctgataaggc aaatcatgca tgggacacga ttcacactgc
     3361 agaatttttc aaaaaagata aattatgtct ggcaaccgga tgtctttgga ttccccgcaa
     3421 cgcttccaca aattctcaaa aaatcaggca ttaactatat tgcatcgcaa aagctctcac
     3481 aaaacaagat taataaattt aataattacc ttttccgctg gcagggcctg gatggctcag
     3541 aaattctgat gcataacttt cctgaagaca cttatgatag ccgagcacgt gctcgctctt
     3601 tagaatatat tgaacaaaac tataatgaaa aagagatctg tccctatgcg cttatggtgt
     3661 atggtgtatg gtgtcggtga tggcggcgct ggccccggtg aggaacatat agaacgatta
     3721 acacgtatac gcaatattga tggcttacct catgttgatt tcagccgtgt tgataagttc
     3781 ttcacttacg ccgacgcctt tcgcgaaagc ttgcccatta tttcaggtga actttatttc
     3841 gaggctcatc aagggtgctt tacatcagag tcagcaacta aagctcataa tcgaaatatg
     3901 gaaaataaat tacatgatgc tg
//
LOCUS       DAETQN010000243         3908 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.247, whole genome shotgun sequence.
ACCESSION   DAETQN010000243 DAETQN010000000
VERSION     DAETQN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.247"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            24..590
                     /locus_tag="K0W68_004374"
     CDS             24..590
                     /locus_tag="K0W68_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBI3075735.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="K0W68_004375"
     CDS             594..1169
                     /locus_tag="K0W68_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBI3075736.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="K0W68_004376"
     CDS             complement(1211..2263)
                     /locus_tag="K0W68_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBI3075737.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="K0W68_004377"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="K0W68_004377"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBI3075738.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3777
                     /locus_tag="K0W68_004378"
     CDS             3580..3777
                     /locus_tag="K0W68_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBI3075739.1"
                     /translation="MSPCGNDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIIPS"
BASE COUNT          971 a          987 c          951 g          999 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta ttcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DAETQN010000244         3900 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.248, whole genome shotgun sequence.
ACCESSION   DAETQN010000244 DAETQN010000000
VERSION     DAETQN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.248"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..196
                     /locus_tag="K0W68_004379"
     CDS             <1..196
                     /locus_tag="K0W68_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075740.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            207..374
                     /locus_tag="K0W68_004380"
     CDS             207..374
                     /locus_tag="K0W68_004380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075741.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            482..715
                     /locus_tag="K0W68_004381"
     CDS             482..715
                     /locus_tag="K0W68_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075742.1"
                     /translation="MDIAVLEIALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLT
                     WQLRKAKPDCKLPDKAMSYLERNGLISVEDILR"
     gene            1007..1162
                     /gene="hokD"
                     /locus_tag="K0W68_004382"
     CDS             1007..1162
                     /gene="hokD"
                     /locus_tag="K0W68_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBI3075743.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1466..1738
                     /locus_tag="K0W68_004383"
     CDS             1466..1738
                     /locus_tag="K0W68_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075744.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1812..2798
                     /locus_tag="K0W68_004384"
     CDS             1812..2798
                     /locus_tag="K0W68_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBI3075745.1"
                     /translation="MSAFHNGRILVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSVYSESDIIPGEQTATSILKQRTKNIALLPHVHQQQNPPQEKTVVS
                     IAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            2799..3179
                     /locus_tag="K0W68_004385"
     CDS             2799..3179
                     /locus_tag="K0W68_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBI3075746.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3176..>3900
                     /locus_tag="K0W68_004386"
     CDS             3176..>3900
                     /locus_tag="K0W68_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBI3075747.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRAS"
BASE COUNT         1050 a          869 c         1086 g          895 t
ORIGIN      
        1 atcaccgaaa aaaattggct tttacaccat tggcgataaa agcatttacc tgtatgacct
       61 acgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcgatgg attggtgtgt
      121 tgctgttcat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
      181 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
      241 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
      301 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
      361 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
      421 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
      481 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
      541 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      601 aaagcagtta acctggcaac tgaggaaagc gaagccagac tgcaaattac cggataaggc
      661 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      721 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      781 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      841 gcttgaggct atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      901 aacattacgc gtccggcaag acgcttaaca ttaatctgag gctcaatcta tgaacggcaa
      961 atctaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     1021 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     1081 gaggaaagac ctctgcgagg tacgaattcg aaccggccag acggaggtcg ctgtcttcac
     1141 agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc cacctctgat
     1201 gggtcaggca tcctcaatgc acccacactt aacccgcttc ggcgggtttt tgtttttatt
     1261 ttcaacgcgt ttgaagtttt agatggtgcc ggaatagaat caaaaatact taagtagcgc
     1321 gcagggagaa gagggatgga ccccgaacag gggagtgcta tttatctgga aggattctgt
     1381 tgatgaaaat cgaagaatta cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg
     1441 ttgagaatgg cgctattgtc agccactgcc ttccagacta cacaattctg ataattcagc
     1501 cgtctttgcc agcaggcacg ggcggcgctc tcacgcattt aaatctgatt ggtaccagca
     1561 tcccccatgc actgaagaac aggccgaatg gctcattcag tgttaccgca ggcgcggatg
     1621 cgaggttaaa aaagccctta gcctcgacta ccgtcactgg ataatctccg tcaggctccc
     1681 ttactccgaa cggccaacgc gtccgtcccg cacattccag caacggatct ggaggtaatg
     1741 tgcgggtatt acttcgacct gttctggtac cggaactcgg tctggttatc gttaagccag
     1801 gccgtgaatc aatgtcagca ttccataacg gcagaatact ggtggagccg gaaccaaaaa
     1861 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgctcg ccagccgctg gtggaagaca
     1921 aaacattgct gccgtttttc agtaacgcac gggtgattcg tgctgctggt ggtgctggtg
     1981 cattgtctga ctggctgttg cgccatatta aatcctgcca gtggccacac ggcgattatc
     2041 atcacagcga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     2101 gcgacaacca gctgcgtgac cagacatccg aatcactcga gcaacttgct catcaaaacc
     2161 tgtcagcatg gatgattgac gtcatcggtc acgcaataag cggtacgcag gagcgtgaat
     2221 tatctctggc tgaattatcc tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg
     2281 aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa aattcgctct gtgtacagtg
     2341 aaagcgacat cataccggga gagcagacag ccaccagcat actgaagcag cgcacaaaaa
     2401 atattgcgtt actgcctcac gtccaccagc aacagaaccc accacaggaa aagacggtgg
     2461 tcagcattgc cgttgatccg gagtcaccgg ctcagtatct ccagcgccag aaatcacaac
     2521 gggaagagat gcctgtatac acgcgctggg taaaaacgca gaaatgcatg acgtgtggca
     2581 atcaggcaga tgatccgcat cacatcattg gtcatggact gggagggatg ggaacaaagg
     2641 ctgatgattt gtttgttatt ccgttatgcc gtaaatgccg taacgaacta catgccggag
     2701 taaaagattt tgaagagaaa cacggcagtc agctgttgtt gctgattcgt tttttaatgc
     2761 acgcgagaaa ttcgggtgtt ctgaagtgga aagcataaat gaccgagcgc atagaatttg
     2821 ttttacctta cccgccgacg gtgaacactt actggcgtcg tcgtggcagc acatattttg
     2881 tatcaaaagc cggggagcgt tatcgccggg cagtggcgct tattgttcgc cagcagcggc
     2941 tgaaattaag cctgtccgga aggttggcaa taaaaattat tgcagaacca ccggataagc
     3001 gccgccgtga cctggacaat attctgaaag cgccgctgga tgcgctgacg catgcggggt
     3061 tgctaatgga cgatgagcag tttgatgaaa tcaatatcgt tcgtgctcag ccagtatctg
     3121 gtggacgtct gggggtgaag atttatccca taatgcttga agggcaggtc aaaaaatgaa
     3181 actggaagat ttaccgaaat actactcccc aaaatccccc ggcctgactg atgcatcggc
     3241 ctcaacgtcg aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg gcatgacaca
     3301 gaatcgggct gagatggggg tttctgcgtt ccttgggaaa atgggcatta gtatgaatga
     3361 cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg atcgcgtggc
     3421 ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta tggcttcgta
     3481 tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgcccct gctgtcacgg
     3541 aaaaaaattt attgaaagcg aggtttttac aaacaagatc cagtatccgg atggtaagcc
     3601 gccggtatgg gcaaagtgta cgaaaggtgt gtatccgtct tactgggaag aatggaaaaa
     3661 agtcagggag gtggtaaaag ttgcctgtcc ggagtgtggc ggaaagggtg aggtttccac
     3721 cgcctgtaag gattgccgtg ggcgtggtgt cgccattcat cgtgaagagt cggtaaaacg
     3781 tggtatgcct gttatcagag actgccagcg ttgtggtggt cgtggctatg aaagactacc
     3841 atcaacggag gcatttaatg ctatatgcga ggtgacaaac cagataacac gcgcgtcatg
//
LOCUS       DAETQN010000245         3866 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.249, whole genome shotgun sequence.
ACCESSION   DAETQN010000245 DAETQN010000000
VERSION     DAETQN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.249"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            752..1468
                     /gene="nanC"
                     /locus_tag="K0W68_004387"
     CDS             752..1468
                     /gene="nanC"
                     /locus_tag="K0W68_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBI3075748.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1488..2594
                     /gene="nanM"
                     /locus_tag="K0W68_004388"
     CDS             1488..2594
                     /gene="nanM"
                     /locus_tag="K0W68_004388"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBI3075749.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2659..3639
                     /locus_tag="K0W68_004389"
     CDS             2659..3639
                     /locus_tag="K0W68_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBI3075750.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         1163 a          801 c          877 g         1025 t
ORIGIN      
        1 gacttcagaa gcgaccagtt acaagccaca aatcaggaaa catatttcat ggtgagaatt
       61 atgatcagcc tgatggcggg taaacacttg ttttcaccac gctcactgag ttagcttgca
      121 aagcttccct tgtttagacc ctaaataaat tttttataaa acaaacagtt aaaagaaatc
      181 ctttagtttt tgaagaccac ctcaagtgtt cgtctggctt cacatggcat cttcctctta
      241 gaaaaagatc gacatatttt gtgacacgaa ttgcaaatct cgttttgttg tatggcttgc
      301 gtgatttttg atctggtata acaggtataa aggtgcaccg cgatagtcaa tgagacaggg
      361 catctcgcaa tctatggcaa acatcacttc agttctttct catcgggtga tgaaaacgca
      421 cttcagtctg aaaggaatat gaaaatgaga tcaacagaca ctctatttta tgactctggg
      481 taaaatggat tgagtaagtg atatagctta cgaacattca aatcaattaa acatcagaag
      541 agattttata ctcaggtatt taatctgatt ctctgtttat ttaaataatg tggaaagaga
      601 attttcacag gagaccttat acaaaaatat aaaataagct acccgttgcc aaagacacta
      661 taagcctggc aaaaaatatt acacaacata aatgctaatt gtttatgcgg gctttgtatt
      721 gctttctgta tcctacaaat gagtgaaatt tatgaaaaag gctaaaatac tttctggcgt
      781 attattactg tgcttttcgt caccattaat ttctcaggct gcgacactgg acgtacgtgg
      841 tggatatcgt agcggaagcc atgcctatga gactcgactc aaagtcagtg agggatggca
      901 aaatggatgg tgggcaagca tggaaagtaa tacctggaat accattcatg ataataaaaa
      961 ggaaaatgcc gcactcaatg atgttcaggt tgaagttaat tacgcgatta aacttgatga
     1021 tcaatggacg gtgcgcccgg gaatgttaac gcattttagc agcaacggca cacgctacgg
     1081 accctacgta aaactgtcct gggacgcgac aaaagatctt aattttggca ttcgctatcg
     1141 ttacgactgg aaagcttacc gacaacaaga cttatccggt gatatgtctc gtgataacgt
     1201 tcatcgttgg gatggatatg tcacttacca tattaatagt gatttcacct tcgcatggca
     1261 aacgacgcta tacagcaaac agaatgatta tcgctatgca aaccacaaga aatgggcgac
     1321 ggaaaatgcc tttgttctac aataccatat gacgcccgat attacgccat acatagaata
     1381 tgactacctt gaccgccagg gtgtttacaa cggcagagat aatttatcgg aaaacagtta
     1441 tcgcattggt gtgtcattta aactgtagta gacaggagac agtcacaatg aataaaacta
     1501 taatggcgct tgctatcatg atggcttcat ttgccgcaaa cgcgtctgta ttaccggaaa
     1561 ctcctgtgcc atttaaaagt ggtaccggag caattgataa cgacactgtc tacattggtt
     1621 taggtagcgc aggtacagca tggtacaagc tagatacaca ggccaaagat aaaaaatgga
     1681 ctgcgttagc tgcattccct ggtggaccga gagatcaagc aacctctgca tttattgatg
     1741 gcaatctata tgtgtttggc ggcattggca aaaacagcga gggattgact caggtattta
     1801 atgacgtaca caaatacaac cccaaaacca atagctgggt taaattgatg tcgctcgcgc
     1861 cgatgggcat ggcgggtcat gtgacttttg tacacaacgg caaggcttat gttactggcg
     1921 gtgttaacca gaatatcttc aatggctatt ttgaagatct caacgaggct ggaaaagatt
     1981 caaccgctat agataaaatc aacgcccact attttgacaa aaaagcagaa gattatttct
     2041 tcaataagtt tctgttgtct tttgatccct caacacagca atggagttac gctggcgaat
     2101 cgccctggta cggaacggct ggtgcggcgg ttgtgaataa aggtgataaa acctggctta
     2161 ttaatggcga agccaaacca ggattgcgaa cggatgccgt atttgaactt gatttcaccg
     2221 gtaataattt aaaatggaat aagcttgatc ccgtctcatc accagatggc gtcgctggcg
     2281 gttttgcggg gataagcaat gattctctta tatttgccgg aggggccgga ttcaaaggtt
     2341 cacgagaaaa ttaccagaac ggtaagaact atgcgcatga aggcctgaaa aaatcatata
     2401 gcactgatat tcatctttgg cataacggga aatgggataa atcgggtgaa ttatcgcaag
     2461 gtcgggccta cggagtatca ttgccctgga ataatagttt attaattatt ggcggtgaaa
     2521 ctgcaggcgg caaagcggtg acggattcag ttttaatttc tgtgaaggat aataaagtca
     2581 cagtacaaaa ctaacgcttc agggccccgg taaaggggcc ctgctatcaa tttgtcattt
     2641 gtattacgcg aaaattatat gaacgcaata atatcgcccg attattacta tgttcttacc
     2701 gttgctggtc agtctaatgc catggcgtat ggcgaaggac tgccattacc ggacagggaa
     2761 gatgcgcctc attccagaat taaacaatta gcgagatttg cgcatacgca tcccggaggc
     2821 ccgccatgtc actttaacga cattattcca ctgactcact gcccacacga tgttcaggat
     2881 atgcagggtt atcaccatcc tctggcaacg aatcatcaaa cacagtacgg caccgttggc
     2941 caggcactgc atattgcacg gaaattactg cccttcattc ctaataatgc agggattctt
     3001 atcgttccgt gttgccgtgg cggatcggct tttaccgcgg gcagcgaagg gacatattca
     3061 gaacggcacg gagccagcca tgatgcttgt cgttggggaa cggatactcc gctgtaccag
     3121 gatttagtca gcagaacgcg agccgcactg gcaaaaaatc cgcagaacaa attcctcggc
     3181 gtatgctgga tgcaaggcga atttgactta atgaccagtg actacgcgtc acaccctcaa
     3241 cactttaatc atatggttga agcctttcgt agggatctaa aacaatacca ttctcagctt
     3301 aataatatta ctgacgcgcc atggttttgc ggcgatacca cctggtactg gaaagaaaat
     3361 ttcccgcatt cgtatgaagc tatttatggc aattatcaaa ataatgtttt agccaatatt
     3421 attttcgtcg acttccagca acaaggtgaa agaggactga cgaacgcgcc tgatgaagac
     3481 ccggacgatt taagcacggg atattacggt tcagcgtacc ggtcaccgga gaactggacg
     3541 acggcactgc gaagcagtca tttcagcacg gcagcccgtc gggggattat ttctgacaga
     3601 tttgtagaag caattttgca gttttggcgc gaaaggtgag cggggagttt attcatgggg
     3661 gatgtccttg ttcgttctca gagtagacaa caaaaaacaa ataagatcaa ggtaatatct
     3721 cttcacttgc gggctcgaac agcaggcaat tgccaatata ataatctggc acaagggtga
     3781 aaaacgcgca ccgcataaac cgttgttatt gcttacagat tagccggatg ccttaataga
     3841 catccgcgcc ttttcgatta tggctc
//
LOCUS       DAETQN010000246         3864 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.250, whole genome shotgun sequence.
ACCESSION   DAETQN010000246 DAETQN010000000
VERSION     DAETQN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.250"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..1803
                     /gene="fimD"
                     /locus_tag="K0W68_004390"
                     /pseudo
     CDS             1..1803
                     /gene="fimD"
                     /locus_tag="K0W68_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1816..2823
                     /gene="fimH"
                     /locus_tag="K0W68_004391"
     CDS             1816..2823
                     /gene="fimH"
                     /locus_tag="K0W68_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HBI3075751.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2834..3349
                     /gene="sfmF"
                     /locus_tag="K0W68_004392"
     CDS             2834..3349
                     /gene="sfmF"
                     /locus_tag="K0W68_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HBI3075752.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="K0W68_004393"
     CDS             complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="K0W68_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HBI3075753.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL
                     IDYAKLYELI"
BASE COUNT          988 a          845 c          959 g         1072 t
ORIGIN      
        1 tggtgtacgg ctttattctt ccgataatat gtatccagat agccagcaag ggtttgcccc
       61 aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat
      121 tatctatcaa agctatgttt cccccggtgc ttttgaaatt actgatttgc acccgacatc
      181 ttcaaatggc gatctggatg tcaccatcga cgagcgcgat ggcagaatta cacaattccg
      241 tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct gacggcgggc
      301 gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg cacggcactc
      361 ggtggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc caattacacc
      421 gcctttttat tagggctggg gcgcaacctc gggaactggg gcgcagtgtc gctggatgtg
      481 acccatgcgc gcagccagtt agccgacgac agtcgtcatg agggggattc cattcgcttc
      541 ctctatgcga aatcgatgta caccttcggc accaattttc agttaatggg ttaccgctat
      601 tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga ggggtacgaa
      661 tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa ttaccacaat
      721 ttacgcttta gccgcaaaga ccgtttgcag ttaaatattt cacaatcact taatgacttt
      781 ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga ttcagatacc
      841 tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact ctcattttcg
      901 tggaatgaat ctgtagggat ccccgataac gaacatattg tcggacttaa tgtttcagtg
      961 cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga ccgcgcttat
     1021 gcctccttga acgctaaccg taacagcaac gggcaaaata gctggctggc aggcgttggt
     1081 gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggcga tacctcgaat
     1141 aatgggtaca cgggcagcgc cacggcaaac tggcaggcca cttacggtac gctgggggtc
     1201 gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg cggtgtggtc
     1261 gggcatgaaa atggcataac actgagccag cctttagggg ataccaatgt tttgattaaa
     1321 gcgcttggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac cgactggcgc
     1381 ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc gcttgatacc
     1441 aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt gccgacgcaa
     1501 ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct cattaccgtt
     1561 acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag taccggaata
     1621 accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt gtctggtgaa
     1681 ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta tgtattgcca
     1741 aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca tcctcgctca
     1801 taaaggaaat tatcaatgaa gataatctgc agattattat tggcaatggc atgtttgtgt
     1861 ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc agaagatgaa
     1921 cactatgatc tctcaaatgt ctttaatagc accaataacc agccagggca gattgttgtt
     1981 ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt gtccactcgg cacgctggtg
     2041 aattatacat accgtagtta tgtcactaac tttattgttc aggaaactat cgataattat
     2101 aaatatatgc aattaaatga ttatctatta ggtgcgatga gtctggttga tagtgtgatg
     2161 gatattcagt tccccccgca aaattatatt cggatgggaa cagatcctaa cgtttcgcaa
     2221 aaccttccgt tcggggtgat ggattctcgt ttgatatttc gtttaaaggt tattcgtccc
     2281 tttattaaca tggtggagat ccccagacag gtgatgttta ccgtgtatgt gacatcaacg
     2341 ccttacgatc cgttggttac acctgtttat accattagtt ttggtggccg ggttgaagtg
     2401 ccgcaaaact gcgaattaaa tgccgggcag attgttgaat ttgattttgg tgatatcggc
     2461 gcatcgttat ttagtgcggc agggccgggg aatcgaccag ctggcgtcat gccgcaaacc
     2521 aagagcattg cgatcaaatg tacgaatgtt gctgcgcagg cttatttaac aatgcgtctg
     2581 gaagccagtg ccgtttctgg tcaggcgatg gtgtcggaca atcaggattt aggttttatt
     2641 gtcgccgatc agaacgatac gccgatcacg cctaacgatc tcaatagcgt tattcctttc
     2701 cgtctggatg cagctgcggc agccaatgtc acacttcgcg cctggcctat cagtattacc
     2761 ggtcaaaaac cgaccgaagg gccgtttagc gcgctggggt atttacgcgt cgattatcaa
     2821 tgaggtacgg agaatgagaa gagtactctt tagctgtttc tgcgggttac tgtggagttc
     2881 cagtggatgg gcagctgacc ctttgggaac gattaatatc aatttgcacg gtaacgttgt
     2941 tgatttctcc tgtaccgtaa acactgcgga tattgataag acggtagatt taggcagatg
     3001 gcctacgaca caattactga acgctggcga taccacggca ctcgtccctt ttagcctgcg
     3061 gctggaggga tgtcctccgg gttccgttgc gattttattt acgggaacgc cggcatccga
     3121 taccaacctg ctggctctgg atgatcccgc aatggcacaa accgtcgcca tcgaattacg
     3181 taatagcgat cgctcccggc ttgcactggg ggaggcgagc ccgactgagg aagtagatgc
     3241 aaatggcaat gtcacactaa acttttttgc caattatcga gcgttagcca gcggtgttca
     3301 gccaggtgtg gagaaagcgg atgcgatatt tatgatcaat tataattaat attatattaa
     3361 ttcgtataat ttggcgtagt cgataagctc tacaattgaa tgcaaaccta gtttgccata
     3421 aatattagat ttatgcgcac taactgtttt attgctaagt aataacttat cggcaatttc
     3481 tttattagat aatccgctaa ccagataacg taatatggtc acttcacgat tagatagcac
     3541 agtgaccgtt gaactattcg tactacattt attgcttttt atatagttga gcgtttcgct
     3601 aggaaaaaac gtgtatccgg agaggatcat ctgaacggga tgaaaaatat cattctgatc
     3661 attgcattta ctgacaaaac cgttagcacc agcttgtatc gctctgccag cataaaagca
     3721 ttctgatttc gatgataaaa ataacacttt cactgtgctc tggatttgtt tgatcctttt
     3781 caggaaggta aaaccgtctg ttccgggcaa gtctatatcc ataatgatta aatcaacagg
     3841 acgggttcgg agataatcga tggt
//
LOCUS       DAETQN010000247         3858 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.251, whole genome shotgun sequence.
ACCESSION   DAETQN010000247 DAETQN010000000
VERSION     DAETQN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.251"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            115..510
                     /gene="caiF"
                     /locus_tag="K0W68_004394"
     CDS             115..510
                     /gene="caiF"
                     /locus_tag="K0W68_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBI3075754.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(719..1309)
                     /gene="caiE"
                     /locus_tag="K0W68_004395"
     CDS             complement(719..1309)
                     /gene="caiE"
                     /locus_tag="K0W68_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBI3075755.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="K0W68_004396"
     CDS             complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="K0W68_004396"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBI3075756.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="K0W68_004397"
     CDS             complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="K0W68_004397"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBI3075757.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          911 a         1049 c          942 g          956 t
ORIGIN      
        1 ttatcgacgg tgaagttgca tactatcgat atatccacaa ttttaatatg gccttgttta
       61 attgcttcaa aacgagtcat agccagactt ttaatttgtg aaactggagt tcgtatgtgt
      121 gaaggatatg ttgaaaaacc actctacttg ttaatcgccg aatggatgat ggctgaaaat
      181 cggtgggtga tagcaagaga gatctctatt catttcgata ttgaacacag caaggcggtt
      241 aataccctga cttatattct gtcggaagtc gcagaaataa gctgcgcagt taagatgatc
      301 cctaataagc tggaaggacg gggatgccag tgccagcgac tggtaaaagt ggtcgatatc
      361 gatgagcaaa tttacgcgcg cctgcgcaat aacagccggg ataaattagt cggcgtaaga
      421 aagacgccgc gtattcctgc cgttccgctc acggaactta accgcgagca gaagtggcag
      481 atgatgttgt caaagagtat gcgtcgttaa tgtcatttcg ttgataccac gcagcctggt
      541 tgctggatgc aatgctggcg catcttatcc agcctacggt tgctctccag ccgggttgga
      601 taaggcgttt atgccgcatc cgacagctat cgccggatgc gatgctggcg catcttatcc
      661 ggcctacggt tgctctccag cagagttgga taaggcgttt acgccgcatc cggcaagatc
      721 accgtttagg cgtcacatcc gtcgtcccct gcaaacgggg gcgattttcc tccatttgcc
      781 tcagtggctg tgtttcatgt aacgatgcat gacagcgccc gacaagatcc tgatactctt
      841 tggtattcaa ccgtttccag tgtaactcgt cgtcactaac actgcgtaca gcgcgggctg
      901 gcgtacccat caacaactgg cgtttctcgc cgtgaaagcc tgctttgaca aagctcatgg
      961 cggcaacaat gctctcttcg ccaatgaccg cgccatccat aatcacgctg ttcatcccga
     1021 ccaatgcatc gcgaccaatc acacaaccat gcaggatcgc tccgtgcccg atatggccgt
     1081 tttccccaac gatggtgtca gtgtcgcagt agccatgcat aatgcagcca tcctgaatat
     1141 tggctcccgc ttgcacgatc aaccgcccgt agtcaccacg cagtgaggcg agtgggccga
     1201 tgtagacacc ggctcccaca atcacatcgc caatcaagac ggcactggga tggacaaacg
     1261 ccgtcgggtg aaccaccgga attaacccct caaaggcgta atagctcacg gttgttaacg
     1321 tcctttccac accggatcgc gcttctcggc aaacgccagc ggcccttcaa tggcatcctc
     1381 cgaatgcaga accgacggat agtgtttcaa cacgccgctg cgaatatagc gatacgcttc
     1441 ttctaccggc atttcgctgg tggtgcggaa gatctctttc agcgccgcaa tcgccagcgg
     1501 ggcgctgtta accagctgct gcgccagttc gcgggcgtta tccatcagtt ccgcctggct
     1561 aaccacgcgg ttgactatcc cccaacgcag cgcctcttct gcgcccattc gtctgccggt
     1621 catcaccatt tcattgacga tggcaggcgg caggatcttc ggtagacgca gcacgccgcc
     1681 gctgtcagga acgatgccca gcttggcttc cggcagggcg aagctggcgt tatcggcaca
     1741 aacaataaaa tctgccgcca gcgccagttc gaaaccgccg tcaaaggcat agccgttcac
     1801 agctgcgata accggtttgt cgagattgaa aatttcggtt aatcccgcaa atccacccgg
     1861 gccaaagtca gcatccggtg cttcgccttc tgctgccgct tttaaatccc agcccgcgga
     1921 aaagaacttc tctccggcac cggtaataat ggcgacacgt aattgcggat cgtcacggaa
     1981 atttagaaat acttcgccca tttcaaagct ggtttttgca tcaatagcat tcgcttttgg
     2041 acgatcaagg gtaatttcca gaattgatcc attgcgggtc agatgtaatg attcactcat
     2101 tccttttctc catttttgct ttttcaggga cgacaacatc cctgcaaaaa atgcatattg
     2161 ttttatagcg tgattattag ttggcagggt agttccctgc tgtttcattt atttcagatt
     2221 ctttctaatt attttccccg agcaattacg tggcagatct tttctgatct ccagataaga
     2281 gggcacttta aatttcgcca tattttgttc gcagaagcgg aaaaattcct cttcgctcaa
     2341 tgtttcacct tcattcagca ccacaaatgc tttgatggct tcatcgcgaa tcgaatcttt
     2401 aatacccaca accacgatat cctgaatttt tggatgggtg gcgatgatat tttccagctc
     2461 cacgcaggag acattctcgc caccgcgttt gatcatattg cagcggcgat cgacgaaata
     2521 aaaaaagccc tcttcgtcgc agtatccggt atcgccggta tgtagccagc cgtcggcttc
     2581 cagcactttc gcagtggctt tcgggttgag aaaatactct ttgaagatgg ttttccctgg
     2641 tacgccttta atacagattt caccgatctc acctgccggg agcgggcgat tgtgatcgtc
     2701 gcggatctcc gcttcgtagc aaaaccccgc ccgaccaatc gacggccagc gtcgtttatc
     2761 gccagggcga tcgccgataa tgcccacaat ggtttccgtc atcccataag acgtcagcaa
     2821 gcgaacgccg aagcgttcac aaaacgcatc tttttcctgc tccgacaagt tgagataaaa
     2881 catcacttcc cgcaggcggt gttgccgatc gttcgctgaa ggcggctgcg ccatcaaggt
     2941 acggatcatc atcggaatac attcggtaac ggtggcgcgg tacttctgta cctgtcccca
     3001 gaaggcgcgg gcgctgtatt tctcgaccag cacaaaggtg gccccggcag aaaacgccgc
     3061 catcgccgca gtacactggc aatcgatatg aaacgcaggc attaccgtca ggtagacgtc
     3121 atcgtcacgc agtgcacact gccaggcgga gtaatatcca gcgaagcgca ggttgtaatg
     3181 ggtaatcacc acacctttcg gtcgggaggt ggtgccggag gtgaagagaa tttccgccgt
     3241 atcgtcagtc gatagcggcg gtgcatagca caaggtggca ggttgttgat ttttcagttg
     3301 agtaaacgaa ctcacgccat catcagcggg aagtgccaca tctgtcaggc aaatgtgccg
     3361 caattgagtg gcatcttcct gctgaatctg ttgatacata ggatagaatt gcgcactggt
     3421 caccagcagg cacgcctggc tattttgcag gatccacgcg ctttcctcgc gcaacaggcg
     3481 ggcgttaatc ggcaccataa tcgcgccaat ttttgccagc ccgaaccagc aaaagataaa
     3541 ttccgggcag ttgtcgagat gtagagcaac cttgtcgcct ttgcgaatcc ccagcgtata
     3601 aaacaggttt gtcgtgcggt taatctcctg atttaactca agataactat accggttaac
     3661 gactccgccg ctggattcac aaatcagcgc cgttttatga ccgtaaacgt ccgccagatc
     3721 gtcccacatt tgacgtagat gttgtccgcc aatgatatcc attgcacctc tatccatttt
     3781 tgttcgtttg ttattgggcg ggcgctagtc aggcaagccg actgacgcca cgcgtttagt
     3841 cctcaacttt ggccagac
//
LOCUS       DAETQN010000248         3831 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.252, whole genome shotgun sequence.
ACCESSION   DAETQN010000248 DAETQN010000000
VERSION     DAETQN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.252"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..265)
                     /locus_tag="K0W68_004398"
     CDS             complement(<1..265)
                     /locus_tag="K0W68_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBI3075758.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            352..759
                     /gene="nudG"
                     /locus_tag="K0W68_004399"
     CDS             352..759
                     /gene="nudG"
                     /locus_tag="K0W68_004399"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBI3075759.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(725..997)
                     /locus_tag="K0W68_004400"
     CDS             complement(725..997)
                     /locus_tag="K0W68_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBI3075760.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            1233..2576
                     /gene="gdhA"
                     /locus_tag="K0W68_004401"
     CDS             1233..2576
                     /gene="gdhA"
                     /locus_tag="K0W68_004401"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBI3075761.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2693..3732)
                     /locus_tag="K0W68_004402"
                     /pseudo
     CDS             complement(2693..3732)
                     /locus_tag="K0W68_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
BASE COUNT          997 a          932 c          917 g          985 t
ORIGIN      
        1 caagagaaat atcaagaaag ccgcccgcgt cggtaagctc tctgcgccgc gccagcgcac
       61 cgtccagacc atcaagcagc ctgttcaaca aaatgacgac tagcgccgcc agataccagc
      121 ccagcgccag aaacggcaga gccagcacgc cgatggcaaa tcccactaat gttaaaccat
      181 ctggcgtaat gcccggttta tcaagaaccc gcacgcactg atgcaacaac ggtttaatcc
      241 ggggatgaag atggcggtct agcacaggca ctccttaaat ataaagcctt tctgattgag
      301 caacagtgcg gatattatgg catttttcgc ttatctgccc gtgtgtaatt tatgaaaatg
      361 attgaagttg ttgccgccat cattgaacgt gatggcaaaa ttttactcgc gcaacgcccc
      421 gcccagagcg atcaggcggg attatgggag tttgccgatg gtaaagtcga gctggatgaa
      481 agccagcagc aggcactggt gcgtgagtta aacgaagaat tgggcatcga agcaactgtg
      541 ggtgagtatg ttgccagcca tcagcgagaa gtttcgggcc ggattatcca tcttcatgcc
      601 tggcacgtac ccgacttcca cgggacgtta caggcacatg aacatcaggc gctggtctgg
      661 tgctcacctg aagaggcgct gcaatatccg ctggcccctg ctgacattcc actattagag
      721 gcgtttatgg ctttacgcgc cgccagagca gcggattagt gctaagggtt ttgtcatccc
      781 gctggcattg cagcagtatt ccttcggctt taattaccgc cccttcagaa taattttgat
      841 cctgataaac gcagcactga gtacagggct gcgctgactg cccgcctgaa ctgaacactt
      901 ctggcggtac gtttacctcc acgtccggac gataatgcgg gttagccagt gcaattaatg
      961 gaaatgctaa tactacggcg aacaatgctc gactcacagg gaactcctta acgttattgt
     1021 ctctgcaact gataacggta gccgggtggc aaaactttag cgtctgagtt atcgcatttg
     1081 gttatgagat tactctcgtt attaatttgc tttcccgggt catttttttc ttgcttaccg
     1141 tcacattctt gatggtatag tcgaaaactg caaaagcaca tgacataaac aacataagca
     1201 caatcgtatt aatatataag ggttttatat ctatggatca gacatattct ctggagtcat
     1261 tcctcaacca tgtccaaaag cgcgacccga atcaaaccga gttcgcgcaa gccgttcgtg
     1321 aagtaatgac cacactctgg ccttttcttg aacaaaatcc aaaatatcgc cagatgtcat
     1381 tactggagcg tctggttgaa ccggagcgcg tgatccagtt tcgcgtggta tgggttgatg
     1441 atcgcaacca gatacaggtc aaccgtgcat ggcgtgtgca gttcagctct gccatcggcc
     1501 cgtacaaagg cggtatgcgc ttccatccgt cagttaacct ttccattctc aaattcctcg
     1561 gctttgaaca aaccttcaaa aatgccctga ctaccctgcc gatgggcggt ggtaaaggcg
     1621 gcagcgattt cgatccgaaa ggaaaaagcg aaggtgaagt gatgcgtttt tgccaggcgc
     1681 tgatgaccga gctttatcgt catctgggcg cggataccga cgttccggca ggtgatatcg
     1741 gcgttggtgg tcgtgaagtc ggctttatgg cggggatgat gaaaaagctc tccaacaata
     1801 ccgcctgcgt cttcaccggt aagggccttt catttggcgg tagtcttatt cgcccggaag
     1861 ctaccggcta cggtctggtt tatttcacag aagcaatgtt aaaacgccac ggtatgggtt
     1921 ttgaagggat gcgcgtttcc gtttctggct ccggcaacgt cgcccagtac gctatcgaaa
     1981 aagcgatgga atttggtgct cgtgtgatca ctgcgtcaga ctccagcggc actgtagttg
     2041 atgaaagcgg attcacgaaa gagaaactgg cacgtcttat cgaaatcaaa gccagccgcg
     2101 atggtcgagt ggcagattac gccaaagaat ttggtctggt ctatctcgaa ggccaacagc
     2161 cgtggtctgt accggttgat atcgccctgc cttgcgccac ccagaacgaa ctggatgttg
     2221 acgccgcgca tcagcttatc gccaatggcg ttaaagccgt cgctgaaggg gcaaatatgc
     2281 cgaccaccat cgaagcgact gaactgttcc agcaggcagg cgtactgttt gcaccgggta
     2341 aagcggctaa tgctggcggt gtagcaacgt cgggcctgga aatggcacaa aacgctgcgc
     2401 gcctgggctg gaaagccgag aaagttgacg cacgtttgca tcacatcatg ctggatatcc
     2461 accatgcctg tgttgagcat ggtggtgaag gtgagcaaac caactacgtg cagggcgcga
     2521 acattgccgg ttttgtgaag gttgccgatg cgatgctggc gcagggtgtg atttaagttg
     2581 taaatgcctg atggcgctac gcttatcagg cctacaaatg ggcacaattc attgcagtta
     2641 cgctctaatg taggccgggc aagcgcagcg cccccggcaa aatttcaggc gtttatgagt
     2701 atttaacgga tgatgctccc cagggaacat ttcttatggg ccaacggcat ttcttactgt
     2761 agtgctccca aaattgcttg tcgtaacgat aacacgcttc aagttcagca tccgttaact
     2821 ttctgcgata gcagcagata tgccagtaaa gaaaccccat tagactattt ttttgataat
     2881 cttcttcgct ttcgaacaac tcgtgcgcct ttcgagaagc aagcattata taatgccagg
     2941 ccagttcttc ttcaattgtc ccgttttgaa aagctgtgct tgatatcgag gtcatccatg
     3001 ataattccgc cgcccatatt agcttcgccg aggatttacc ggagcgatta ttagcgcaat
     3061 cagagatata gtctgaggga aaaacagcaa atttattcaa caaggcgata acctgctctg
     3121 gggcttcctc catgtttgct ttaaaggtat tggctccatg gtcgccagaa agaaaatgct
     3181 ccattaggca caataacttt cgctatcttc gataccccat tgattctcta aagactcgcg
     3241 tcttttactg atgatatcga tcgagtcaaa aggaagcaca tgatattgga aggtatcttt
     3301 accaggttca ggctttcgcg gccagaactc tagcgtttca gaccattgct tatgatagaa
     3361 tcgataaggt gcgatcaatt gtagcgcctg taacttctcg atactgagcg gctcaatacc
     3421 tttagcctga taataatgca gttgttcttt ttttgcttta aaaccggccc gaacaataag
     3481 ccccatcata attaatagat aaagaaaaga gcatcccgcg gtaatcaggc ctctttcatt
     3541 caaaccgttg gatgttatcg ctgcgaacac aaacattaca gcgacaacac atgttaaata
     3601 aaacccccac ttacaaagca gcatggcctt attttcttta atcatccgtt caaaattact
     3661 attaaatatt tcccagccat taaaagaata cttctcgctc ccaggatggt tttgtaataa
     3721 aacttttttc atcacattcc ctgttattac atactttaga ggcgataatt ttatccatgc
     3781 aaaaaaaata tccaacgaat tttcttgatc cgctggatat ttcagaatat g
//
LOCUS       DAETQN010000249         3797 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.253, whole genome shotgun sequence.
ACCESSION   DAETQN010000249 DAETQN010000000
VERSION     DAETQN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.253"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(98..907)
                     /gene="proC"
                     /locus_tag="K0W68_004403"
     CDS             complement(98..907)
                     /gene="proC"
                     /locus_tag="K0W68_004403"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBI3075762.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1027..1485
                     /locus_tag="K0W68_004404"
     CDS             1027..1485
                     /locus_tag="K0W68_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBI3075763.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1668..2192
                     /gene="aroL"
                     /locus_tag="K0W68_004405"
     CDS             1668..2192
                     /gene="aroL"
                     /locus_tag="K0W68_004405"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBI3075764.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2242..2433
                     /gene="yaiA"
                     /locus_tag="K0W68_004406"
     CDS             2242..2433
                     /gene="yaiA"
                     /locus_tag="K0W68_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBI3075765.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2691..3368
                     /locus_tag="K0W68_004407"
     CDS             2691..3368
                     /locus_tag="K0W68_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBI3075766.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3440..3724
                     /gene="ppnP"
                     /locus_tag="K0W68_004408"
     CDS             3440..3724
                     /gene="ppnP"
                     /locus_tag="K0W68_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBI3075767.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          904 c          990 g          952 t
ORIGIN      
        1 cgtgagtggt tgaaatcggc agatttggcg ctttacaaag caaagaaagc cggacgtaac
       61 cgcaccgaag tggcggcctg acgtccggcg aaagtcatca ggatttgctg agtttttctg
      121 atttttccat acacttcgtc atcgcttcga tcactgcggc acggaagcct ttctcttcca
      181 gtacgcgtac cgcttcaatg gtggtgcctc ccggtgagca gaccatatct ttcagtgccc
      241 ccggatgttc tcccgtttcc agcaccattt ttgcggaacc cattaccgcc tgagcggcaa
      301 atttatacgc ctgggcgcgt ggcatcccgc ccagcacggc ggcgtcggcc atcgcttcga
      361 taaacataaa tacgtaggct ggcgaagaac cgctcacacc gaccaccggg tggatcatcg
      421 gctcagcaat tacttccgct tcgccaaagc agcggaaaat attcagcaca tcagcggtat
      481 cttcgggggt taccagcgcg tttggcgtta cggaggtcat cccggcatta accagcgcgg
      541 gagtgttcgg catggcgcgg ataattttcc ggtcatggcc cagcgcgcgg gcaagctggt
      601 cgagcgtgac acctgcagca atagaaacga ccagagagtc tttattcagg ctggaggtga
      661 tttcgctaag cactttaatc atgatgccag gtttaacggc ggcaaaaatg atgtcggcga
      721 tttgcgccac ttcttgcgcc gattctgcgg cgttgatgcc gaactggtca tgcagggcgg
      781 cgactttatc cggggagggg gtgtataccc agatttgccc tggaagcacc tgaccgctgg
      841 caatcagacc gccgagaatg gcttttccca tattgccgca gccaataaaa ccgattttct
      901 tttccattgc ctcactcctg ccgtgaaatt cattgttttg ataatcgctg gcagaagcat
      961 aaacagaact atgccggaag gcaaaagcgc gacacaatag aggattaccc aacaaaggat
     1021 gactttatga caatttgggt ggatgccgac gcgtgtccca atgtaattaa agagattttg
     1081 tatcgcgcgg cggaacgtat gcagatgccg ctggtactgg tagcaaacca gagtttacgc
     1141 gtgccgccat cgcgatttat tcgtacgctg cgcgtcgcgg caggtttcga cgttgccgat
     1201 aacgaaattg tccggcagtg tgaagcgggc gatttggtga tcaccgcaga tatacctttg
     1261 gctgctgaag ccatcgagaa aggcgcagcg gcgcttaatc cgcgcggcga acgttacacg
     1321 ccagcgacca ttcgtgagcg cctgacgatg cgcgatttta tggatacctt acgtgccagt
     1381 gggatccaga ccggcggacc agatagcctt tcacaacgtg accgccaggc ctttgccgcg
     1441 gagctggaga agtggtggct ggaagtgcaa cgtagtcgtg gctaaatgta atttattatt
     1501 tacacttcat tcttgaatat ttattggtat agtaagggat gtattgagat tttcacttta
     1561 agtggaattt tttctttaca atcgaaattg tactagtttg atggtatgat cgctattctc
     1621 atgacaccgg ctttcgccgc attgcgacct attggggaaa acccacgatg acacaacctc
     1681 tttttctgat cgggcctcgg ggctgtggta aaacaacggt cggaatggcc cttgccgatt
     1741 cgcttaaccg tcggtttgtc gataccgatc agtggttgca atcacagctc aatatgacgg
     1801 tcgcggagat cgtcgaaagg gaagagtggg cgggatttcg cgccagagaa acggcggcgc
     1861 tggaagcggt aactgcgcca tccaccgtta tcgctacagg cggcggcatt attctgacgg
     1921 aatttaatcg tcacttcatg caaaataacg ggatcgtggt ttatttgtgt gcgccagtat
     1981 cagtcctggt taaccgactg caagctgcac cggaagaaga tttacggcca accttaacgg
     2041 gaaaaccgct gagcgaagaa gttcaggaag tgctggaaga acgcgatgcg ctatatcgcg
     2101 aagttgcgca tattatcatc gacgcaacaa acgaacccag ccaggtgatt tctgaaattc
     2161 gtagcgccct ggcacagacg atcaattgtt gattttagag cgcctatact taacgttcat
     2221 cccgtgaaat aaggaagaac gatgccaacg aaaccgcctt atcctcgtga agcatatata
     2281 gtgacgattg aaaaaggaaa gccaggacag acggtaacct ggtaccaact cagagccgat
     2341 catcctaaac cagactcgtt gatcagtgaa catccgacgg ctcaggaagc gatggatgcg
     2401 aaaaaacgct atgaggaccc tgacaaagag tgaccgcatc agactgctcg gaagggattc
     2461 tgagtgccac tacaagggat ctgcgtcaca tttttcataa ttcatgtttt tctaataatt
     2521 agaatattaa acaataacaa tccattactg gaatcatttg gaatctttac attatgccgt
     2581 gcacgtctgc tgctacgctt tttgtcattt gtagcacaag taagtgtcag cagtggtgct
     2641 ccacacttgc ccggtaatta acgacgaaag aaaagtaagg tggatgaaca atgagtgcgt
     2701 cgttggcgat cctgaccatc ggcattgtac ctatgcagga ggttttgccg ctcctgacgg
     2761 aatacattga cgaagataat atttcccatc atagcctgct ggggaagtta agtcgtgaag
     2821 aagtgatggc ggagtacgcg ccagaagcag gcgaagacac cattctcaca ttattaaatg
     2881 acaaccagct ggcccatgtt tcgcgtcgta aagtggagcg agacctgcaa ggtgtggttg
     2941 aagtgctcga taatcagggt tatgacgtca ttttattaat gagtacagca aacattagta
     3001 gtatgactgc gcgtaatacg atctttcttg agccgtcgcg aatattgcct ccactggttt
     3061 cctctattgt tgaagatcat caggtggggg ttatcgttcc ggttgaggag atgctgaccg
     3121 ttcaggcgca aaaatggcaa attttgcaga aatcgccggt attttcattg ggtaacccca
     3181 ttcatgattc agaacaaaaa atcattgatg ccgggaaaga attactggca aaaggggctg
     3241 atgtcatcat gctggattgt ttaggatttc accaacgtca tcgcgattta ctgcaaaaac
     3301 agctcgatgt tcctgtcttg ctgtctaacg tattgattgc acggctggct gcggaattac
     3361 tggtgtaatt ttgcgtgaca gccagcgcct ctggccccta tagtgaagta gatgttcaac
     3421 taccagacag ggccagttta tgcttcaaag taatgagtac ttttccggca aagtgaaatc
     3481 aatcggcttt tccagcagca gcactggtcg cgccagcgtg ggtgttatgg ttgaaggcga
     3541 atacaccttc agcaccgctg agccggaaga gatgacggta atcagtggcg cgctgaatgt
     3601 gttactgcct gacgcgaccg actggcaggt gtatgaagcc ggttcggtgt ttaatgttcc
     3661 cggtcacagt gagtttcatc tgcaagttgc cgaacccacc tcttatctgt gccgctatct
     3721 gtaattcctc gccttcccct tgaacgggag ggcatttttc tgaaatatcc tctctttaac
     3781 ccataataat atttcct
//
LOCUS       DAETQN010000250         3735 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.254, whole genome shotgun sequence.
ACCESSION   DAETQN010000250 DAETQN010000000
VERSION     DAETQN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.254"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..182)
                     /locus_tag="K0W68_004409"
     CDS             complement(<1..182)
                     /locus_tag="K0W68_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HBI3075768.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            426..683
                     /locus_tag="K0W68_004410"
     CDS             426..683
                     /locus_tag="K0W68_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HBI3075769.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(733..1683)
                     /gene="uspE"
                     /locus_tag="K0W68_004411"
     CDS             complement(733..1683)
                     /gene="uspE"
                     /locus_tag="K0W68_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBI3075770.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="K0W68_004412"
     CDS             complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="K0W68_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBI3075771.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="K0W68_004413"
     CDS             complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="K0W68_004413"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBI3075772.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3308..>3735)
                     /locus_tag="K0W68_004414"
     CDS             complement(3308..>3735)
                     /locus_tag="K0W68_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBI3075773.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1003 a          886 c          893 g          953 t
ORIGIN      
        1 ataatgctac caacagcact gataattatg gtgtagatta aaaattgcac cgcatggaat
       61 aaaaaacctt ttcttttacg atttccgcgg cgaaaccaaa agctcatcag aatcaatgct
      121 gcgcagctac cgaaaataat gaccagatta agcgcattat ttgtaaacag ttcagcgatc
      181 attgttttat caggctcctc cagataattg tcgtcatgcc ggaaacccct ggcggggcta
      241 ttttaccgcg acaattcatt cagatcatca atagtcaggg aaggaagtag caacattagc
      301 taactaatca atgcgttgat tgtaaatcca gctaagaggt gaggttttca gagcagacaa
      361 cggtgaaatg tcatggtatt gttacgttta ggtaacaaga aatttgtctg cacaaggatt
      421 acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa aaaatttctc tttggtttgt
      481 tggcggtttt cattattgcg gcgtcggttg tgactcgcgc gactatcggc ggcgttatag
      541 aacagtacaa tattccgctg tctgagtgga cgacatcaat gtatgtgatt cagtcatcga
      601 tgatttttgt ttatagcctg gtctttactg tgttgctggc aatcccgttg ggaatttatt
      661 tccttggcgg cgaagagcag taagtaaaaa ataggcccga taactcgggc cttgtcagtt
      721 attgaagagt cgttaatcgt cttcttcgtc atccagttca acgggtgtct gatactggtc
      781 aggtttaatg accaacagat cgcagcgaag atgatcaatc acctgttccg ccgtgttgcc
      841 gaggaatgct gctgaaatac cggtgcgtcc taccgtgccc agaaccacaa tccccgcctg
      901 taagtgctcc gccaaatcag gaataacctc ttccggcaga cctttttcta cgtgcgtcat
      961 gttttcatta atgccgaatt tctgccgcag agctttcatt gccagcaaat gttgcccacg
     1021 aatggcatcg ttataaacgc tcggatcaaa ttccggcagt tcaatcgcga tattaattgg
     1081 cgttactggg taagcgccaa ccagatgaac ttcggtatgg ttgacttgtt ctgccagttc
     1141 gatcgtctct ttgaccagtt tttcattgag cgcattatga tacggctctt cactggcgag
     1201 attcaccgcc accagcgcct tacctccttc cggccacggc tggtctttca ccatccacac
     1261 cgggcttggg catttgcgta acagatgcca gtccgttggc gtaaaaatca ccgcttccag
     1321 acggtcatgt tggtgcgcca tttttagcac caaatcgtgc ccgccgctga tcacttcctg
     1381 aatgatggct tcgaagggac ggttatgcca gaccacttta atttcaatgg gaacgccagc
     1441 attgagataa tattttgcct gctcgtggat ccaggctgta cgctggctga tgacgccctg
     1501 acgcatagcg gtacgttcgt ccggggagag cagggtggtc atttcgtatg agaagtcata
     1561 gatcggcaaa aaggctttaa ttttgccacc aatccgttga tgtaaataaa cagctcgccg
     1621 caatgctggt tggtcgtcct ggttaggatc gataacaacg agcatgttct gatacatagc
     1681 catacagggt ctccttacaa caactgtcaa cgcagtttgt aattaaaaga ttaacccata
     1741 tctggtgaat gaaacagtga tgaaccttct gccagatcaa taaatcagaa aaatttaatg
     1801 atatgacaga aggatagtga gttatgcgga aaaatcaggc aacattacgc gtatgaccag
     1861 caagctgggc cagcgcatcg ttattttcga tggtgatgta tttacctttg actgccagca
     1921 taccgctttt ctggaagcga cctagcagac ggctgatggt ttctaccgtc aggcccagat
     1981 agttaccgat atcgccacga gtcatcgtca ggcggaattc acgaggggag aagccgcgtt
     2041 gggcaaaacg acgggacagg ttgtagatga atgcagccag acgttcctcg gcatttttct
     2101 tcgacaacag caagatcatg tcctgatcgc ctttgatttc accgctcatc agacgcatca
     2161 tctgctgacg cagattcggc attttaccgg acaaatcgtc cagcgtttcg aacgggattt
     2221 cacataccat cgaggtttcc agcgcctgag cgaagctcgg gtgatggccg ctgccgatgg
     2281 cgtcaaatcc caccaggtcg ccagctaaat ggaaaccagt gatttgctcg tcgccttgct
     2341 cagtgatggt ataactttta atcgtaccgg agcggatggc ataaagcgat ttaagttcat
     2401 caccagcctt aaacagcgtc tggcctttct gaataggctt cttccgctca atgatattat
     2461 caagctgatc aagctcatgt tcgttgagtg tgaacgggat gcaaagctgg ctgatgctgc
     2521 aatcctggca atggatagca caaccgccag actgaatgcg ccgtataatt cgcttttccg
     2581 ggatcatagg tctgctcaag ccgtaattga tatttgtcaa ttttaacatc tttttaggga
     2641 gcaagtaagt ctaagcaaac cttaacagca gagaattccg atattagatg taaatatatg
     2701 tctatctatt tgaaaaccct taagttgtta agggtaactt tacataaaag tgtgaacaag
     2761 ctggcacaaa ttgtttaatg tttacagcaa aagataacct tcgtggcgca ataaccactc
     2821 ttttcgctga actccgcctg catatccggt catggtgccg tttcggccaa taacccgatg
     2881 gcaaggtacg acgatgctga tgggattcga tccgtttgcc gcaccaacgg cacgcgccgc
     2941 gccagggcgg cccaattgtt cagccagttg gccgtaatgc attacctgtc cgcaggggat
     3001 agtgcgtaga gttttccaga cttcgcgctg aaatggcgtc ccccctgtgg cagtgggaag
     3061 cgtatcaata atgctaagat taccggcaaa ataatcacga agcttgtcgc ttaaaccgcc
     3121 tgggttggtg gcagaaatgc gctcatagcc ttctttgcga taatggatgt ccagcagctg
     3181 caccatgcgt tcgctgtact cttcccattc aaccgcccgc aggcgaaatt gctcatcgca
     3241 aatcacccac agtggaccca gtggcgtggc aattttttct tcaagtaatc tcagcatccg
     3301 ttctctctta agacaaacgt gggtaaatcc ccggacctat cggcagacca acaagatacc
     3361 acgccagcaa catcagcagc cataccacca aaaagataag cggatagggc aagactaacg
     3421 aatagtaagt acccagtttc gcgtctggtt tgtagcgttg caggaatcca agaaacagtg
     3481 gaacaaaagg agataccggc gctaaaggca atacggatga gtcggcaata cgaaagagga
     3541 tttgcgcaaa tgccgggtga aagccaagta gcataaacat tggtacgaaa atgggggcca
     3601 gaatcgacca gattgcggaa ccgctggcaa taaacatgca taagaaagag gaaagcaacg
     3661 ccagaccgac aaacgccggg atgccgctaa gccctgaact ttccagtata tcggtcagcc
     3721 ccacggcgat gaatt
//
LOCUS       DAETQN010000251         3674 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.255, whole genome shotgun sequence.
ACCESSION   DAETQN010000251 DAETQN010000000
VERSION     DAETQN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.255"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..95
                     /locus_tag="K0W68_004415"
     CDS             <1..95
                     /locus_tag="K0W68_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3075774.1"
                     /translation="RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            244..1491
                     /locus_tag="K0W68_004416"
     CDS             244..1491
                     /locus_tag="K0W68_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HBI3075775.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2020..2220)
                     /gene="torI"
                     /locus_tag="K0W68_004417"
     CDS             complement(2020..2220)
                     /gene="torI"
                     /locus_tag="K0W68_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HBI3075776.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2343..2687)
                     /locus_tag="K0W68_004418"
     CDS             complement(2343..2687)
                     /locus_tag="K0W68_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075777.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2929..3531
                     /locus_tag="K0W68_004419"
     CDS             2929..3531
                     /locus_tag="K0W68_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075778.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          919 a          740 c          845 g         1170 t
ORIGIN      
        1 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
       61 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc cgcttgcagt
      121 aacggcgaaa ggggcaatga cagcgctgcc atgtcgacaa gaactggaat agtgagaagt
      181 aaacggcgaa gtcgctctta tctctaaata ggacgtgaat tttttaacga caggtaggta
      241 attatggcac tgaatattcc attcagaaat gcgtactatc gttttgcatc cagttactca
      301 tttctctttt ttatttcctg gtcgctgtgg tggtcgttat acgctatttg gctgaaagga
      361 catctagggt tgacagggac ggaattaggt acactttatt cggtcaacca gtttaccagc
      421 attctattta tgatgttcta cggcatcgtt caggataaac tcggtctgaa gaaaccgctc
      481 atctggtgta tgagtttcat cctggtcttg accggaccgt ttatgattta cgtttatgaa
      541 ccgttactgc aaagcaattt ttctgtaggt ctaattctgg gagcgctctt ttttggcctg
      601 gggtatctgg cgggatgcgg tttgcttgac agcttcaccg aaaaaatggc gcgaaatttt
      661 catttcgaat atggaacagc gcgcgcctgg ggatcttttg gctatgctat tggcgcgttc
      721 tttgccggca tattttttag tatcagtccc catatcaact tctggttggt ctcgctattt
      781 ggcgctgtat ttatgatgat caacatgtgt tttaaagata aggatcacca gtgcgtagcg
      841 gcggatgcgg gaggggtaaa aaaagaggat tttatcgcag ttttcaagga tcgaaacttc
      901 tgggttttcg tcatatttat tgtggggacg tggtctttct ataacatttt tgatcaacaa
      961 ctttttcctg tcttttatgc aggtttattc gaatcacacg atgtaggaac gcgcctgtat
     1021 ggttatctca actcattcca ggtggtactc gaagcgctat gcatggcgat tattcctttc
     1081 tttgtgaatc gggtagggcc aaaaaatgca ttacttatcg gtgttgtgat tatggcgttg
     1141 cgtatccttt cctgcgcgct gttcgttaac ccctggatta tttcattagt gaagctgtta
     1201 catgccattg aggttccact ttgtgtcata tccgtcttca aatacagcgt ggcaaatttt
     1261 gataagcgcc tgtcgtcgac gatctttctg attggttttc aaattgccag ttcgcttggg
     1321 attgtgctgc tttcaacgcc gactgggata ctctttgacc acgcaggcta ccagacagtt
     1381 ttcttcgcaa tttcgggtat tgtctgccta atgttgctat ttggcatttt cttcctgagt
     1441 aaaaaacgcg agcaaatagt tatggaaacg cctgtacctt cagcaatata gacgtaaact
     1501 tttccggttg ttgtcgatat ctccctatcc ctcaaccgga aaataataat actaaagtgc
     1561 ttagcccggc taataatcac ctaatccaaa cgcctcattc atgttctggt acagtcgctc
     1621 aaatgtactt cagatgcgcg gttcgctgat ttccaggaca ttgtcgtcat tcagcgacct
     1681 gtcccgtgta tcacggtcct gcgaattcat caaggaatgc attgcggagt gaagtatcga
     1741 gtcacgccat atttcgctat caggattctg tgtgatggtt acatcgcccg gcccagggct
     1801 gtttagtcat cagcgctttc tgacagtgct gagatttcaa cctgttgcag taaaaatgag
     1861 tagatataag gcaagtgtgc tgccaaaccc atcttttacg gggtgaaggt agatttcgtt
     1921 tgaagggtat ctggtgtccc ctgcagacat ctacttgagg cggcagggga ttgattggaa
     1981 tgctgttttt tagatttgaa aaatatttta cccgctattt tacccattgg cgcggcttaa
     2041 gagcttattt ttgaattcac aatggtcacg atataaccat cttgctcgtc cgtggataac
     2101 tttggcttta ggcaggtcgc cggacttaat ccggtcatag atgaaggtct taccgaagcc
     2161 agtatcagcc atgatgaatt tcaaatcaac cagtgaatca ggttgtagtt cgtgttgcat
     2221 gagtgctatc tccgaatatg gaatcgaacc tgcaaatcag gcaataaaaa gccgcattga
     2281 tgcagcaatg gtaggtctgg atatcttgag aaatgaacag gcctcatcga gtgtgaggcg
     2341 ggttagtcct tgcgtagctc gctgattctt ctgtaagtct ctggtgcttt gttcccgtac
     2401 gtcttcattt cagacttcaa cagagcaacg agtgaatccc attcgttgag gattcctttg
     2461 aatgccggaa cgcgctttgc aaccttgtcg aatgaatctc tgatttctgg aatctgctca
     2521 acaagtgcaa cgcatcgccg aaagtctgct gcgtcatgga gagcgccgaa gtgatgacca
     2581 tagatattct ttttcaggcc acatgcgatt gaggcaagag ttgcgctact gatgccgaca
     2641 tcgccagttg attgccattt caaaaccttc atagccaaat ctgacatttc ttgtctccat
     2701 aaaacaaaac tcgccgtagc gagctcagat aaaagaaatc cccgtcagtg cgaggatgct
     2761 gttcattgct gctatacact tttttgctct caacgtaagc ggtagcccat tctgttgggt
     2821 tggtcagttg cttttaggaa atgctcttta ccccttaaac gtcggctgaa agagctaaaa
     2881 tccatgcaaa aaatttacgc aattttgtgt attattgtgc agtaagtaat gagctatttt
     2941 ctgtgcaaaa aatggatgat aaatttgtcc ggggcaggaa aaattttatg ggcgctaaac
     3001 atgaaaaaag attcgtatcc ttatttgatt tgcatgacag tttcagggct gatctttatt
     3061 ttccttttct tctggtggcg ggcagatatc tacagggtca cgtttcttaa tcagagtata
     3121 tcccactatt acattctgtt tagcatggga atagcttttc tgttatctct gttttgggtt
     3181 aagaagggga tagtaaaaca aagcggctgg aagagtctgt cagcatacct taaggtttat
     3241 gcagggatat gcatattttc tggatttttt ctgattatac cccttacaac actaacttat
     3301 tttttgcctg gagagacatc gtcttatgtt gcaccgtatc ggtatacttc cggtagttca
     3361 aaaagttgtt ctggagctga ggtggatgac cccgatctac atgagaatat tcgcatttgc
     3421 tatccgtatg gcaattatga gtacgataat attatctatg ttgaaaagaa aattaataca
     3481 ttaggtgcgg tagtgacata tgcacagacc gcgcgtgatg atactgaatg agatagtata
     3541 tagcgggcaa gttttagtta atttatcgag gtaatataat ttacctcgac tcgtttattc
     3601 tggtatgaat atttcgcttt acgatcgatt tttatctgat gatatcatgc ggttttcata
     3661 tactgactta ctgt
//
LOCUS       DAETQN010000252         3588 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.256, whole genome shotgun sequence.
ACCESSION   DAETQN010000252 DAETQN010000000
VERSION     DAETQN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.256"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            30..383
                     /locus_tag="K0W68_004420"
                     /pseudo
     CDS             30..383
                     /locus_tag="K0W68_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
     gene            623..883
                     /gene="repA"
                     /locus_tag="K0W68_004421"
     CDS             623..883
                     /gene="repA"
                     /locus_tag="K0W68_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBI3075779.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1107..1181
                     /gene="tap"
                     /locus_tag="K0W68_004422"
     CDS             1107..1181
                     /gene="tap"
                     /locus_tag="K0W68_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBI3075780.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1174..2031
                     /gene="repA"
                     /locus_tag="K0W68_004423"
     CDS             1174..2031
                     /gene="repA"
                     /locus_tag="K0W68_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBI3075781.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESDAGTLSITRATRALTFLAELGLITYQTEYDPLIGCNIPTDITFTPALFAALDV
                     SEEAVASARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2731..2922
                     /locus_tag="K0W68_004424"
     CDS             2731..2922
                     /locus_tag="K0W68_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075782.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTAACRLT
                     CRWRGIRIIQAAFNAFAKV"
     gene            2995..3246
                     /locus_tag="K0W68_004425"
     CDS             2995..3246
                     /locus_tag="K0W68_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBI3075783.1"
                     /translation="MPNIILSETSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            3243..3530
                     /locus_tag="K0W68_004426"
     CDS             3243..3530
                     /locus_tag="K0W68_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBI3075784.1"
                     /translation="MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
BASE COUNT          953 a          879 c          914 g          842 t
ORIGIN      
        1 actaaatcag taagttggca gcatcaccga agcggtggta tctgtgtccg caggtacggg
       61 tcgcggatat cgtccagctg aacgggaata tccggccacg atcacgccag tggtggcagt
      121 tattcaggat ggtgtctcag tggcatgttg atgtggttat cgttgagctg cgttcgttca
      181 gtattgttgc agcagtagag ctggatgatg ccagccattt acgaccggaa cgcagacgcc
      241 gggatattct tctggaagag gttctgaggc aggctggtat tccgttgctc agaagccacg
      301 atgccagaaa actgctgcag atgaccggag aatggctgaa tacaacaggg gctgatcagc
      361 agtcaccgga acatcgtagc tgacgccttc gcgttgctta gttgtccaac cccggaaacg
      421 ggaaaaagca agttttcctc gctcccggcg tttccataac tgaaaaccat actatttcac
      481 agtttaaatc acattaaacg acagtaatcc ccgttgattt gtgcgccaac acagatcttc
      541 gccacaattc tcaagtcgct gatttcaaaa aactgtagta tcctctgcga aacgatccct
      601 gtttgagtat tgaggaggcg agatgtcgca gacagaaaat gcagtgactt cctcattgag
      661 tcaaaagcgg tttgtgcgca gaggtaagcc tatgactgac tctgagaaac aaatggccgc
      721 agttgcaaga aaacgtctta cacacaaaga gataaaagtt tttgtcaaaa atcctctgaa
      781 agatctcatg gttgagtact gcgagagaga ggggataaca caggctcagt tcgttgagaa
      841 aatcatcaaa gatgaactgc agagactgga tatactaaag taaagacttt actttgtggc
      901 gtagcatgct agattactga tcgtttaagg aattttatgg ctggccacgc cataaggtgg
      961 cagggaactg gttctgatgt ggatgtacag gagccagaaa agcaaaaacc ccgataatct
     1021 tctttaactt tggcgagtca gaaagattac cggggcccac ttaaaccgta tagccaacaa
     1081 tcaagctatg cggggagtat agttatatgc ccggaaaagt tcaagacttc tttctgtgct
     1141 cgctccttct gcgcattgta agtgcaggat ggtgtgactg atcttcaaca aacttattac
     1201 cgccaggtaa agaacccgaa tccggtgttc acaccccgtg aaggtgcccg gacgcttccg
     1261 ttctgcggga aactgatgga aaaggcggtg ggcttcacct cccgttttga tttcgccatt
     1321 catgtggcac atgcccgttc gcttggtctg cgtcggcgca tgccaccggt actacgtcgc
     1381 cgggccattg atgcgctgct gcaggggctg tgtttccact atgacccgct ggccaaccgc
     1441 gttcagtgct ccatcaccac gctggccatt gagtgcggac tggcgacgga gtctgatgcc
     1501 ggaacacttt ccatcacccg tgccacgcgt gccctgacgt tcctggcaga gctggggctg
     1561 attacctacc agacggaata tgacccgctt atcggctgca acattccgac cgacatcacg
     1621 ttcacaccgg cgctgtttgc cgccctcgat gtatcagagg aggcggtggc gtccgcgcgc
     1681 cgcagtcgtg tggaatggga aaacagacag cgcaagaagc agggactgga taccctgggt
     1741 atggatgaac tgatggcgaa agcctggcgt tttgtgcgtg agcgtttccg cagttaccag
     1801 acagagctta agtcccgtgg aatgaagcgt gcccgtgcgc gtcgtgatgc ggacaggcag
     1861 cgtcaggata ttgtcaccct ggtgaaacgg caactgacgc gtgaaatctc ggaagggcgc
     1921 ttcactgcca gtcgtgaggc ggtaaaacgt gaagtggagc gtcgtgtgaa ggagcgcatg
     1981 attctgtcac gtaaccgtaa ttacagccgg ctggccacag cttccccctg aaagtgacct
     2041 cctcagaata atccggcacg cgccggaggc attcgcacgc ctgaagcccg ccagtgcaca
     2101 aaaaatcagc gctacataca aaaaacaacc tcatcatcca ccttctggtg catccggtcc
     2161 cccctgtttt tgatacaaaa cacgcctcac agacggggaa ttttgcttat ccacattaaa
     2221 ctgcaaggga cttccccata aggttacaac cgttcatgtc ataaagcgcc agccgttagc
     2281 tttacagggt gcaatgtatc ttttaaacag ctgtttatat ctcctttaaa ctacttaatt
     2341 acatccattt aaaaagaaaa cctgttcact gcctgtcctg tggacagaca gatatgcacc
     2401 tcccaccgca agcggcgggc cccgaccgaa gccgatttag ttacaacatg caaacataaa
     2461 cccctaaaaa acccaagtcc gacgcagaac tgaaaccaca acgcccctcc tcataactga
     2521 aaagcgccgc tgcccccgcc cgaagggcgg gaacagagtc gcttttaatt atgaatgttg
     2581 taactacatc gtcatcgctg tcagtcttct ggctggacgt atcgaataca cgctcgtaag
     2641 cggccctgac ggcccgctaa cgcggagata cgccccgact tcgggtaaac cctcgtcggg
     2701 accactccga ccgcgcacag aagctctgtc atggctgaaa acgggtatgg cttagcaggg
     2761 ctggggatgg gtaaggtgaa atctgtcaat cagtaccggc taacgccggg cttcggcggt
     2821 tttactccgg tatcacatgt aaccgcagcg tgccgcctta catgccgctg gcgcggcatc
     2881 agaataatac aagcagcatt taatgcattt gcaaaagtgt gagtaataaa gacttgttaa
     2941 gtggtcgttt cagggatatc gttaagactt gttaaacagt ctcaaaggtg cgttatgcct
     3001 aacatcattc tgtctgagac cagtgcaagt gtcagtgaac tgaaaaaaaa tcctatggct
     3061 acagtcagtg ccggagatgg ttaccctgtc gccattctta accggaatca gccggcattc
     3121 tactgtgtgc ctgctgaact gtatgaaagg atgcttgatg ctctggatga ccaggaactg
     3181 gtgaaactgg ttactgaacg cagcaatcag ccactgcatg atgtggacct ggacagttat
     3241 ttatgactta tacggtaaaa ttcagggatg atgcgctcaa ggaatggctg aaactggata
     3301 agaccattca acaacagttt gtgaaaaaac taaaaaaatg cagcgaaaat cctcatatac
     3361 cgtcggcaaa actaagaggg ctaaaggact gctacaaaat taaactacgt gcatcgggat
     3421 ttcgtctggt ttatcaggtg attgacgata tgttaattat tgcggttgtt gcggtaggaa
     3481 aacgagaacg tagtaacgtt tataatctcg ccagtgagag aatgagatag taacatataa
     3541 ataacctttc ccgcagataa ctcagaattg ttattttctc tgtggact
//
LOCUS       DAETQN010000253         3584 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.257, whole genome shotgun sequence.
ACCESSION   DAETQN010000253 DAETQN010000000
VERSION     DAETQN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.257"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            63..387
                     /locus_tag="K0W68_004427"
                     /pseudo
     CDS             63..387
                     /locus_tag="K0W68_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(417..3329)
                     /locus_tag="K0W68_004428"
     CDS             complement(417..3329)
                     /locus_tag="K0W68_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075785.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPKGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3332..3448
                     /locus_tag="K0W68_004429"
     CDS             3332..3448
                     /locus_tag="K0W68_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBI3075786.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
BASE COUNT          898 a          963 c          882 g          841 t
ORIGIN      
        1 ggcaaaggcc atgaaagccc tgatttccgg aagcgagcga acagtccaca aactgttgaa
       61 cgtggtgtca gcggcggaca gggataagcc ctcgtcaaca gttccgtcag cactgcgaca
      121 gcgttcttta cccggtcaaa acagcgttgc ggtgcaccac gcctgacgga tgaactgcgt
      181 gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg
      241 agggcaaagg cctcccggaa gttcagcccg gtcagttacc gcgcacatgg cctgcccgtg
      301 tcagaaaatc tgctggagca ggatttttac gccagtggtc cgaaccagaa gtgggcggga
      361 gacatcacgt acttacgtac agatgaagtt cggttacatc ctgtcagcac agaacctcac
      421 gctttttgag cggcacgacg gatccccccc acccagtatc cgacagactt ttcactgcgt
      481 tcgtaaaaac ccttcttccc tcccagcata tccactaatt ccggtgacat atcctgcaga
      541 taagtctgca aatcatgccc cagatcttcc ggtgactgca actcataaac cgcgcgccac
      601 acatcaccct gccttatttt aagccccggg ttaagaatat actgtccgtg gttcagcctc
      661 agaaaaagct tttttccata aggatttttg ctgttaactt catgttgaga gagctggctg
      721 ctgatataac tccttcgttt gcggcgttcc ggcagcaacg cttcaggaag atgaaccaca
      781 cactctgcca gtcgtgccgc actgaaggct tccgaaaaaa acagagcact tcccagatgc
      841 gtgtggaaca gcgcctgcat caggatcacc agaaacattg ccatctgccg gttgtccagt
      901 ttgatgaggc gtccttccac ctgcagtgac agactttcag gtttcagcag ggtatacaac
      961 tgcgcacttt tctgttgcgc ataacggggc gtactgaccg ccgctgacag aacttgatga
     1021 taagccgcca gcccgtcatt accggtcagc aagggatctg caccacgctc cagcagtaac
     1081 tgaaccagtg caatattacc tgcccgggct gctgacatca gcggagtaag attaaacggc
     1141 gaacggtgat caacaccata acgttcaata tcccgcaaca cggcaaccgg gttattcatt
     1201 gagtatggcc ggaaatactt cagctccagt aactgcagag tcttcgttct gggctgcctt
     1261 gtgggcttaa aatcttcccg gtacagtgca ttatacagcg ggaacaggga ataaatcaga
     1321 ctgtactcca gcgcctgcag ttgtatggtt ttatcggctg tacctgcgag gatatgctgt
     1381 ttccactgtc gtgcatcctc tgcggtgata atctgccatg gcatctcaga tgtctgcagg
     1441 atccggctac ggatggtgtc cgcctgctcc tgcttcccct gtttttcaag cagatgtgcg
     1501 gttttctgcc actcatcacg gctggaaatc tcttcctgca gattcagagg ctcttcctga
     1561 tgcgtgagcg caagcagact ccacagagga tgctccacct gctgttcaac aagatacaca
     1621 ttgtgtgtag cacgggtgag cgcaacatac agcgcgttgg taaaaaattt atagatttcc
     1681 gctgaacgat cctgcctgtc ccgcggacgg gaataacggg cctcgccctc aagatcagcc
     1741 ggtgtaagac cctcgcaaat atcatcaaat gcctgccggg cagcgcttac tatattgtaa
     1801 aggatcaccg tttcgtattc cagcccctta acctcctgaa ccgaaaagac cagcggcgta
     1861 ctgaaccagc atcgcgcctg tgctttctgt tccggatgca tcacaatgac cgctacacga
     1921 ttcgaaagtg aggttttggc attcagttcc tgctttgtct cttccctgtc gtccagcaac
     1981 cggatggtcc cttctgcctg accgcagctt ctgacaaaat ggtgactttc ccgatcaata
     2041 gcactaaagc ggacctgttt gagtctcagc aaccggttag ccagtgcagt gacgtggtgc
     2101 ccgttacggt aattcgcctg caggatatta acggttgtgt cgtttccctg ttgctgccgg
     2161 aaaaagagac ttttgagcga actccatgag aaaaagctcg gatgaactat ctgattggca
     2221 tcaccacaga gtaaaaactg accgggatga cgtagcgttt tcagtaccag ttgcagttga
     2281 acaggcgtca tatcctgaac ttcatcaaca aaaatgacat cataacgggg agttgccaac
     2341 gaaagatact gatgactgag cagattactg tcatactgat gcgactgttt cagccatgcg
     2401 atataacgtt caaacaggac atagaccgtc gggcggtcct ccatgccata gagcgactgc
     2461 cgtatcccca gtgacagata cgcctcccgg gacagcggcc cattcccgga ggcaaccgca
     2521 ccgatcacgc ccctgaactc ctcatacagg gtatgggcag cccccagtgc ggcgatagcc
     2581 ctgttgcggg gcagccagtc gctgaacgca ctgaaagtca cctccctgcc tttcggaatg
     2641 cgcagagttt ccagaaattc cgtcagcgaa agaaagtcaa tattctgtac ctcactccct
     2701 tcccctgaag cgtcatagag agtacgcgct ttttccacca gaaatgagga aaggctgaga
     2761 tacagtatat caccagcagc ctgcttcatt ttttccagta ccagcgccgt cttaccactt
     2821 cccgccgagc caacaatcac cagcggtagc gggtgttcgt acagtgcctg ctgatcatca
     2881 tcaaaggaca gcattttatc caggcggtgg aaacgcccat gagacggatt gatatacgtc
     2941 agcgcttcag tcgcgatatc aacaggatcc ggcactggtt gttgttgtaa gcgcccttca
     3001 tcaatcacca cgtttctgcg aagaaaacgt gaagtgttat aggcatgatt gcgaatatat
     3061 tccagaacaa ggcagatagt ttcaccgtgg tagcgataca gcgaaaacag taatcggtcc
     3121 cggatactga gccgggcgcg gtaaagattg gtgtcaattt tacgaacatc agcctgaaca
     3181 aagttatcgg tggcaatagc ctgtgcaaat tttttataac ccgggatcgt gtcaggattc
     3241 agagtatgat aaagacagat tttcatggct gccggtttcc gtcgtgcaaa aaacatgctg
     3301 attttacgaa ggaatgcctt actgaacaac cttgcccgga ataccccccg aaatcagatg
     3361 gaacaatcag aaaaatgctc tgcagttatg gaataaagaa ggtggggtgg tacgccggga
     3421 gggcgatccg ctggaaatgg cgcagtagct gcaggcctgt catgatgccg gaatagaagt
     3481 tcgtgtacag gttaatgaaa gcgtcacccc gtaaataaac tctgcgctga aattcctttt
     3541 tttcattgga gcacctgtgt tgttctgagg tgaacatatc acct
//
LOCUS       DAETQN010000254         3546 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.258, whole genome shotgun sequence.
ACCESSION   DAETQN010000254 DAETQN010000000
VERSION     DAETQN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.258"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..143)
                     /locus_tag="K0W68_004430"
     CDS             complement(<1..143)
                     /locus_tag="K0W68_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075787.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVL"
     gene            complement(189..530)
                     /locus_tag="K0W68_004431"
     CDS             complement(189..530)
                     /locus_tag="K0W68_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075788.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(699..890)
                     /locus_tag="K0W68_004432"
     CDS             complement(699..890)
                     /locus_tag="K0W68_004432"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075789.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(823..2154)
                     /gene="xylB"
                     /locus_tag="K0W68_004433"
     CDS             complement(823..2154)
                     /gene="xylB"
                     /locus_tag="K0W68_004433"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBI3075790.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQKLDYRTGGGCGASTGRSKAGADCGESREIAH"
     gene            complement(2226..>3546)
                     /gene="xylA"
                     /locus_tag="K0W68_004434"
     CDS             complement(2226..>3546)
                     /gene="xylA"
                     /locus_tag="K0W68_004434"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBI3075791.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          924 a          983 c          784 g          855 t
ORIGIN      
        1 agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca tagccctaac
       61 agataggtaa cgataccacc aacgagagct atccatgaca caatactaaa ggccggtgaa
      121 taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt gtaagagctt
      181 tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac aaaaaaggcg
      241 atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc gataaacaag
      301 agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt atccagttgc
      361 ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat taacacgcca
      421 agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac cagataaacc
      481 agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat aacgatctcc
      541 atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaag aatgccatta
      601 acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag atatatagat
      661 gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt
      721 tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca
      781 tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct
      841 ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc
      901 cgtacggtaa tcgagcttct gaccgctgat atccgccagc atctgacgcc agtactcact
      961 acgcgccccg ccaccaatca acgtaacact ttgcggttta atgccgcagg catgcacgac
     1021 atccatgcca tctgccagcg cataacccac gccttccagc actgctcgcg ccagttcatt
     1081 ggggccatgt tgatgagtca aaccaaagaa aacccccttc gcctggggat tattgtgtgg
     1141 tgtacgctcg ccggaaagat aaggcagaaa ccaaactggc tcgacacttt catcagcctg
     1201 ttgagctgca gcgattaaag ctgggacatt gcacaggccg gttaatttcg cggcccaatc
     1261 cagacacgat gctgcactca gcattacaga cattaaatgc caacgctgcg gtagcgcatg
     1321 gcaaaagctg tgtacagcgc tttctggctt gcttaagaac ccttcgctga cagcaaaata
     1381 gacccccgac gtccccagcg ataacattgc ctgattagca tcaaccattc ccacaccaac
     1441 tgcaccagct gcattgtcgc caccgcctgc gacaactggc accgtcgcca taccccacgc
     1501 tttcgcaact tcaggtaaca aagcaccagt aatttcgctg ccttcgtata atgcgggcat
     1561 ctggtcacga gataagtcgc aagcctgcag catgacgtca ctccagtcac gctttgcgac
     1621 atccagccac atggtgccag ctgcgtcaga catatcgctg gcaaactccc ccgtcatacg
     1681 cagacgcaag taatctttcg gtaataatac tttgtcgatt tgacggaata tctccggctc
     1741 atgccgctga acccatagca atttaggcgc agtaaatccg ggcatcatca ggttgccggt
     1801 aatcactcgt gattgcggaa ctctcgcttc cagcaaagtg cactcttgcg cacagcgccc
     1861 gtcgttccac aaaatggcag ggcgcaatac ccgttgttga gcatccagta aggttgctcc
     1921 atgcatctgg ccggcaatac ccaatgcttt aacgtcctgc agagaatgct gatcgcccag
     1981 agctttcatt gcgcgatcag ttgcctgcca ccactgttcc gggtcttgtt ccgaccagag
     2041 tggatgcggg cgcgaaacgg tcagcttttc cgtttgcgaa tcaaccacct caccctgctc
     2101 gttgagcaaa ataactttta cgcccgaggt gccaagatct atcccgatat acatatcgat
     2161 cgttccttaa aaaaatgccc ggtatcgcta ccgataaccg ggccaacgga ctgcacagtt
     2221 agccgttatt tgtcgaacag ataatgattt accagatttt ccagttgctc ctggcgacca
     2281 ctctgatgca ccggagacaa attatgttcc tgagcatatt tggctaaatc tgccagtgac
     2341 atttggcctt tcaggatttg ctggcccaat tcgctattcc agccggaata acgctgcgcg
     2401 atgcgtttat ccagctcgcc atcttcaatc atgcgcgctg caattttcag cgccagtgcc
     2461 atcgtatcca tcgcgccgat atgaccgtaa aacagatcat atttatcagt actttgacga
     2521 cgtactttgg catcgaagtt cagaccaccg gtggtgaaac cgcctgcttt gagaatttca
     2581 tacatcacca gcgcattctc ttccacactg ttcgggaact ggtcggtgtc ccagcccagt
     2641 tgcgcatcgc cacggttggc gtcgacagaa ccgaacaggc caagcgcaat ggcggtggct
     2701 atttcatgat ggaaagagtg acctgccagc gtcgcgtggt tagcttcaat gttcagttta
     2761 atctcttttt ccagaccaaa ctgtttcagg aagccataga ccgtcgcggc atcgtaatca
     2821 tattgatgtt tagtcggttc ttgcggtttc ggttcgataa gcaacgtgcc ctggaagccg
     2881 attttatgtt tatgctcaac caccatctgc ataaagcggc ccagttgttc acgctcctga
     2941 cgcaagtcgg tatttaacag cgtttcgtaa ccttcacgac caccccacag gacatagttt
     3001 tcaccgccca atttatgggt tgcttccatc gctgtaacaa cttgcgttgc cgcccagctg
     3061 aagacttcag gatctgggtt cgtcgccgca cccgcgccat agcgagggtt tgtaaagcag
     3121 ttagcggttc cccacagcag cttcacgccg ctctcttctt gcttggctgc caggacatca
     3181 accatttgcg caaaattatt gatgtactct tttaacgacg cgccctcagg ggaaacatcc
     3241 acatcgtgga agcaataaaa tggcacatgt aacttgtgga aaaactcaaa tgcgacatct
     3301 gctttacgct tcgccaacgc cagtgcctca ccaggctgct gccacggacg attaaacgcc
     3361 cccacaccaa acatgtccgc cccgttccag cagaaggtgt gccagtagca ggcggcaaaa
     3421 cgcaagtgct cttccatacg cttacccaac accagttcgt cgggattgta gtgacggaat
     3481 gctaacgggt ttgaggattt tgagccttca taacgaacgc gatcgagctg gtcaaaatag
     3541 gcttgc
//
LOCUS       DAETQN010000255         3446 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.259, whole genome shotgun sequence.
ACCESSION   DAETQN010000255 DAETQN010000000
VERSION     DAETQN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.259"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            532..2385
                     /locus_tag="K0W68_004435"
     CDS             532..2385
                     /locus_tag="K0W68_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBI3075792.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            2536..2751
                     /locus_tag="K0W68_004436"
     CDS             2536..2751
                     /locus_tag="K0W68_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBI3075793.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2756..3070
                     /locus_tag="K0W68_004437"
     CDS             2756..3070
                     /locus_tag="K0W68_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HBI3075794.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
BASE COUNT          855 a          825 c          938 g          828 t
ORIGIN      
        1 ttccaggccc atccgctccg gtgggccttt ttactgcaga aaacaggttc cccgttaaat
       61 gctatgttgc tcacaattca gtaagttgac agttgcctgt cagactgggc atttgttaaa
      121 aaaatttcgc atggtgaatc cccctgagcg gaggggcgac tggtgacggt ataatctctg
      181 attatcaaaa cgagaatgac gcgggtttag tggcaccggg ctgaactcac cgggaggcac
      241 ccggcaccat gtgcatgatg atacagatac gcggctttag cccctctccg gaggggtttt
      301 cttgtgggca aaaaaagccc gcgctgggag acgcgggcgg caaggaataa acaataaaac
      361 gtgaagtaat atttcagctg gcgaataata ccccatagta atcactctgc gcaactgcgc
      421 ggtctttttc gaattgcggg ctgtcgtctc tcttctgcca ttgtcctgta acttccggac
      481 ttcagcccgc tccttatttt actcacaata ttatcccggc cgggaggatt catggcattt
      541 aaacactatg acgtggtcag ggcggcatcg ccgtcagacc ttgcgaaacg actgacacaa
      601 aaactgaagg agggctggca gccgtttggt agtccggtgg ccataacccc ttataccctg
      661 atgcaggcga ttgcagcaga aggtgatgtg gtcgtcagtg gtgcaactga gccggagtgg
      721 tactacgtca tcgtactggc cgggcaatcc aatgccatgg cttacggtga aggtcttccg
      781 cttccggatt catacgatgc gccccatccg cgcattaagc aactggcccg tcgtaacaca
      841 gtgactcccg gtggtgaagt atgcgtattt aacgacatca ttcctgctga ccattgtctg
      901 catgatgttc aggatatgag tacgattaac catccccggg ctgacctgag caaagggcag
      961 tacggctgtg tcggacaggg cttacatatt gccaaaaaac tgcttccgta tatccctaat
     1021 aatgcgggga tcctgctggt accatgctgt cgtggtggtt cggcattcac ccagggcacg
     1081 gaggggacat tcagcgagtc cacgggggcc agtcaggatt cggctcgctg gggagtgggt
     1141 aagccgttat atcaggatct gcttttccgc acgaaggcag cattgcagaa aaacccgaaa
     1201 aacgttttgc tggcgatatg ctggatgcag ggggaattcg atatgacgaa tgccagttac
     1261 gcccagcagc cagcagcatt tcttgcaatg gtacagcagt tccgtgctga ccttgccggg
     1321 ctggcggcgc agtgtcacgg tggaagtccg gcatcagtcc cctggatttg tggcgacacg
     1381 acatacgcgt ggaaacaaga acacggtacg caatatgaag tggtatatgg tgcatataaa
     1441 ggtaaagaat cccagcagat ttattttgtt ccctttatga ccgatggtag cggagttaat
     1501 acaccgacaa acaacccgtc agaagatcct gatattgccg ggtctggtta ttacggttcg
     1561 gcatcccgaa cgaacaaaaa ctgggtatca tcaaatcgcc cgacgcattt cagctcatgg
     1621 gcgcgtcgtg gcattattcc cgatcgtatg gcaactgcta ttctgaacgt agccggtcgc
     1681 accttagcct tcattagtgg taaggcaccg gaaatcaaac cctcgcccgg cggcgacacg
     1741 ccatcggggc cgtctgaaga tgcatccata cgcacaatct ccctgttgcc gacagccgga
     1801 gacgctgctg cgcagggctg gagcattaag aatggcggag ttcagttgtc agatggtgta
     1861 tttaagatca ccaagcagag caataaagcc tggtccctga cgcgcccggt ggatgacgca
     1921 gtctccctgc tgacacgggg tggcagactg agctgtaagt ttcgactgtc aggcgcactg
     1981 accaacaacc agttcggtct gggaatttat ctgtataccg atgtagcgtt acctgacgtc
     2041 gtggcgatga ccgggactgg taacccgttc ctgatgtcgt tcttcaccca gaccacagac
     2101 ggcaaactga atctgatgca tcacaagaaa gcaggaaaca caaagttggg cgagttcggg
     2161 aattacagta acgactggct gacgctggag ctggtgttca ccgccggcag tgccacggtt
     2221 actccgaaac tgaatggagt ggctggcccg gcattccagg tcataaaaga cagtctgaca
     2281 ctggggctga atgcgctgac gctgacggat attaccaaaa atgcagcgta tggcgttgag
     2341 atagaaagtc tggtgctgga gataaatgca ccggcatcat cataaaaagt gagccagcca
     2401 aatggaaggt atcgttaaac tcaccggtag tgtcagtggg tcgtctgaga tgcctgcatg
     2461 agttatcaga gccatcagta cttaactggt ggcttttttt attgttgtca gcttccggat
     2521 aacgggagac ggggtatgta ccagatggaa aaaatcacaa caggtgtgtc atacaccacg
     2581 tcagcggtgg gaacgggcta ctggttcctg cagttgctgg acagggtttc cccgtctcag
     2641 tgggcggcaa taggcgtgct ggggagtctg ctgtttgggc tgctgacata tctgactaac
     2701 ctgtatttca aaatcagaga ggaccgtcgt aaggctgcac ggggagagta attcaatgac
     2761 tcaaaactat gaactgattg tgaaagggat ccgcaatttt gagaataaag ttacggtaac
     2821 tttagcgtta cgggacaaaa aacgctttga cggtgaaatt tttgacctgg acatctcgct
     2881 ggaccgtgtt gaaggtgccg cgctggagtt ttatgaggca gcagccagaa ggagcatcag
     2941 acaggtcttc ctggatgttg ctgccgggtt atgtgaaggg gacgagctgt tgccagaaac
     3001 gcgcccctgt tcagaggcgc ggtataccat aaaaattaac agttctgata actcgattac
     3061 gggttgttag ctttttgcag ttggctttcc agtatctttc attggtagca tcctgataaa
     3121 tatccatgag cgcaaaaatc aaatacggcc tgtcagctgc tgttctggcg ctgattgcaa
     3181 aatggcatct gaatcgatcg cagtttggca ttcgaatcga tcatgatttg gcatccatct
     3241 ccgatcacag tttggcatca caatcgatca cgatttggca tacttccgat cattgattgg
     3301 aattcgccag tcactccggg aattaactct tttcaccaca gtctccattg ccgtgtttaa
     3361 accagtggag acgtcaatgt ccaaaaagag aaaatccagg agcactatgg aaacttgttt
     3421 taagatcctt cagctcaagt tcgata
//
LOCUS       DAETQN010000256         3431 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.261, whole genome shotgun sequence.
ACCESSION   DAETQN010000256 DAETQN010000000
VERSION     DAETQN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.261"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..229)
                     /locus_tag="K0W68_004438"
     CDS             complement(<1..229)
                     /locus_tag="K0W68_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075795.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKK"
     gene            403..1116
                     /locus_tag="K0W68_004439"
     CDS             403..1116
                     /locus_tag="K0W68_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBI3075796.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            1148..1906
                     /locus_tag="K0W68_004440"
     CDS             1148..1906
                     /locus_tag="K0W68_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075797.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1952..3310
                     /locus_tag="K0W68_004441"
     CDS             1952..3310
                     /locus_tag="K0W68_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBI3075798.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGVMTP
                     TY"
     gene            complement(3304..>3431)
                     /locus_tag="K0W68_004442"
     CDS             complement(3304..>3431)
                     /locus_tag="K0W68_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3075799.1"
                     /translation="RHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          781 a          828 c          889 g          933 t
ORIGIN      
        1 atttttttgc tgttttcacc tgatttcgtt caattgtttt atgtaaggat tttcccatca
       61 acggtaattt tgtcactgca cgaccgacat ttccggcctg ttttcctaaa tgaactcttt
      121 cggcagcacc atatttttcg acaacggtaa taagatgttc acacaccgtt gacttgcctg
      181 aaccatcgct accaataacg gcaattaatg gaggtgttat tgactgcatg aatatatcct
      241 ttaatttaaa catccattaa aaatatttat ttggttaata tgtttttatg aaagcgtaat
      301 tcaggtcaat gtcacaatta accattgtca caataaggtt gaacggatat tctgagcacg
      361 gaccacgtaa tattcaatac attatttact gccgggtttt tcgtgaatca acgtaatatg
      421 tcaataatta attccacgcc agtgcgtgtt attgccattg taggatgtga tggttcaggt
      481 aaatcgcccc tcacggcaag cctggtaaat gaactggcag caagaatgcc aacagaacac
      541 atttatctcg ggcaatcgtc cgggctaatt ggcgaatgga tttcacagct ccctgttatt
      601 ggcgcacctt ttgggcgtta tctgcgaagt aaagcggcac atgtgcacga aaaaccctca
      661 acaccgcctg gcaatattac tgctctggtt atctatctgc tttcctgctg gcgggcgtac
      721 aagtttcgca aaatgttgtg taaaagccag caaggctttc tgctcatcac cgaccgctac
      781 ccgcaggttg aagtgccggg ttttcgcttt gatggcccgc aattggcaaa aaccacgggc
      841 ggtaacggtt ggataaaaat gttaaggcag cgcgagctga agctgtacca atggatggca
      901 tcttatttgc ccgtattgtt gattcgtctt ggcattgatg aacaaaccgc gtttgcgcgt
      961 aaacctgacc accaactggc agcgttacag gaaaaaatcg ccgttacgcc gcaactgact
     1021 ttcaacggcg ccaggatcct tgaactggat ggtcggcaac cagccgatga aatcctgcaa
     1081 gcgtcactac gtgcaattca cgccgcgctt tcctgatcac caaaatggat aggggatgat
     1141 ttaacgaatg gatgcactac aaacacaaac tgttaatagt acaaccgcgc cgcagcccag
     1201 ctacattccg gggctaattg cggtggtcgg gtgcgatggc accggtaaat ccacgctgac
     1261 caccgatctg gtgaaatcat tgcaacagca ctggcaaacc gagcggcgct atctggggct
     1321 gctctcgggc gaagacggcg acaaaatcaa acgattaccg ttggttggcg tctggctgga
     1381 acggcgactg gcggccaaat cctcgaaaac ccaaagcatg aaaaccaaat ctccggcgct
     1441 atgggcggcg gtgattatgt actgcttatc gctgcgaaga atggcgaatc tacgcaaggt
     1501 tcagcgactg gcgcaaagtg gcgttctggt ggtcagcgat cgcttcccgc aggctgaaat
     1561 ttcgggcttt tattacgacg gaccggggat tggcgtcgaa cgtgcgaccg ggaaaatcag
     1621 catgtttctg gcgcagcgcg aacggcgttt ataccaacaa atggcgcaat atcgcccgga
     1681 attaattatt cgcctgggca ttgatattga gactgccatc tcccgcaagc ctgaccatga
     1741 ctatgccgag ctgcaggaca aaatcggtgt catgtcgaag attggctata acggcacaaa
     1801 aattcttgag attgattcca gagcgcccta cagcgaagtg ctggaacagg cacagaaggc
     1861 ggtttctctg gttgccatcg tttctgaccg ccgaagttta acttaaggct ggcgggatac
     1921 tttttactat caacgataag gtcaggctga attggcgggt tttaacatca aacattggtt
     1981 tgcagatggc gcgtttcgca ccattattcg caatagcgcc tggttaggct ccagtaatgt
     2041 cgtgagcgcc ttgctggggc tgttggcgct ctcgtgtgcc ggtaaaggga tgacgcccgc
     2101 catgtttggc gtactggtga ttgtgcaatc gtacgccaag tcgatcagcg attttattaa
     2161 gtttcagaca tggcagctgg tggttcagta cggaacgcca gccttgacca acaataatcc
     2221 gcagcaattt cgcaatgtcg tctcattttc cttctcgctg gatatcgtca gcggcgcggt
     2281 ggcgattgtc ggcggcattg ccttactgcc attcctttcc cattcattag gtctggatga
     2341 ccaaagcttt tggctggcgg cgctgtattg cacgctcatt ccttcaatgg cttcctccac
     2401 gccgaccggc attctgcgtg cggtagatcg cttcgattta attgctgtac agcaggcgac
     2461 gaaacctttt ctgcgcgcag cggggagcgt cgtagcctgg tattttgact ttggttttgc
     2521 gggttttgtt attgcctggt acgtgtcgaa tctggttggc ggcaccatgt actggtggtt
     2581 tgcagcgcgc gaattacgcc gcagaaatat ccataatgcc ttcaaattga atctgtttga
     2641 gtctgcccga cacatcaaag gcgcgtggag ttttgtctgg tcaaccaaca ttgcccactc
     2701 catctggtcg gcgcgtaact cgtgcagcac agtgctggtg gggatcgtgt taggacccgc
     2761 tgccgccggg ttatttaaaa tcgccatgac attctttgac gccgccggaa cgccagcggg
     2821 tttgctgggt aaaagttttt acccagaggt gatgcgttta gatccgcgca ccaccagacc
     2881 gtggttgtta ggtgtgaagt ccggtttact ggcgggcgga atcggtattc tggtggcgct
     2941 cgcggtgttg atcgccggca agccgctcat ttcgctggtg tttggcgtta agtatctcga
     3001 agcgtatgac ctgattcagg tgatgctggg cgcaattgtg atttcgatgc tgggtttccc
     3061 gcaggagtca ttgctattga tggcgggaaa acagcgcgct tttctcgtgg cgcaaaccat
     3121 cgcctcaatc ggctacatcg tattgctgtt catgttctgt catctgtttg gtgtgctggg
     3181 agcagcgttt gcttacttcg gtgggcaatg tctggatgtg gtgctctcgt taattccaac
     3241 tttaaaggca ttttttcagc gtcattcctt gctttataac gcagccgggg taatgactcc
     3301 aacttattga tagtgtttta tgttcagata atgcccgatg actttgtcat gcagctccac
     3361 cgattttgag aacgacagcg acttccgtcc cagccgtgcc aggtgctgcc tcagattcag
     3421 gttatgccgc t
//
LOCUS       DAETQN010000257         3367 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.262, whole genome shotgun sequence.
ACCESSION   DAETQN010000257 DAETQN010000000
VERSION     DAETQN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.262"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..262)
                     /locus_tag="K0W68_004443"
                     /pseudo
     CDS             complement(1..262)
                     /locus_tag="K0W68_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(377..1015)
                     /gene="ospI"
                     /locus_tag="K0W68_004444"
     CDS             complement(377..1015)
                     /gene="ospI"
                     /locus_tag="K0W68_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HBI3075800.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1174..1644)
                     /locus_tag="K0W68_004445"
                     /pseudo
     CDS             complement(1174..1644)
                     /locus_tag="K0W68_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1980..2165
                     /locus_tag="K0W68_004446"
                     /pseudo
     CDS             1980..2165
                     /locus_tag="K0W68_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2265..2474)
                     /locus_tag="K0W68_004447"
                     /pseudo
     CDS             complement(2265..2474)
                     /locus_tag="K0W68_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(2524..2703)
                     /locus_tag="K0W68_004448"
                     /pseudo
     CDS             complement(2524..2703)
                     /locus_tag="K0W68_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            3281..>3367
                     /locus_tag="K0W68_004449"
     CDS             3281..>3367
                     /locus_tag="K0W68_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3075801.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQR"
BASE COUNT          933 a          839 c          701 g          894 t
ORIGIN      
        1 gcaagcgctg taactctttt atttgtttta tagcggcagc cagttctgag gcaggaacga
       61 catcctctcc ggcagcaaca gcagtgagac ttccttccag atattatttt ttccacttaa
      121 gcaacaggct gggctgaata ccatgcaaac gcgccacatg ggacaccgtc atgccaggct
      181 ccattgtctg ctgaataatg gcaatttttt cctgaggagt tttacgttta cgaagctcca
      241 gccccagcaa agttccagtc atctcaaaat tagaactagt gttagacata tatccaagcc
      301 tatctcttat tttaagatac atccactgtc tggcgtttca aggggccggc tcaaatctat
      361 gtacaggctc tcagcatcag caaagcctct tactcttcca actactactg ttagcatagt
      421 gatcaaattc ctgatcagat acatgaatta cctgaaccga actattttca tctacaaatg
      481 catctggcga tttttctaca ggatcataaa gattaaatcc ctttcctgtt tgaacatcca
      541 aaaacataat tttttcacca tcgttaaatc cagctatcca atgagtggcc tgttcaatag
      601 aaatcattac tacacttcct tttgaagtgt tttgtaaaat attttctgca atacgctcac
      661 gaaatttact ataagtttca ttaggatgtg acactatgcc ttcactatca atttgctcta
      721 aatgcctatg aaaaaatccg gtcatatact ctgatatttc attaggtacg ggagcctctc
      781 ttactccata ccctaacata gccatacaag catgaagcgc acaccctgaa cagttaccat
      841 ccccaccaga cggatttatc attttctgta aagtcgctgc caattcagaa tctttatcca
      901 tatcaccaag acttttagag tttaaagatt ccagcaattt tttaacattt acatttccct
      961 cctctgtatg tgcctcatat ccagcagttc cctgtaacga caccccatta atcatattgt
     1021 atcctgacag tatatatact cactatattt tcttcataac aaatatccct ctgatatgac
     1081 aacaaaaaac ggagccggat tcctgttttt gtgaactgca gctattcgag tctaaaaagt
     1141 gatccactta ccaccaacgg cctaatatat acagaacgga gtaaacaggg ctaacagttc
     1201 ccgacaggtc tcatcattcc gtggcccgaa agtgtaagcc aacacaccgc cagttttagt
     1261 gttgtacgca taccagagcc agtgttgttg cagccttgct gccaacgaag cgccactgct
     1321 catcaagttc acagataagg gcaacatcag cgtgagcgac tggcgcagat gttatccgcc
     1381 ggggcgggag ctttttaatg tccgtatgac ggtattgatg ccgattttca gtgtcctggc
     1441 ggtatcacgg ccccctgcgc cgttatgtgc catttcaacg atctgctcct taacgccggg
     1501 ttttctggct tcataagagt aagtgagctg gaaaacacgt ttacatgagc gacaacggaa
     1561 acgctcatgc cgggaacaac tacggccatg tcgataaact tcatcggaat gacagcgcgg
     1621 acaatgaaca gtaacggtag ccatggaaaa ccttaaatac cagtagatta ccagaaactt
     1681 ctgtcgattg aagacatcac ccgaagtttg aattcattcg aatacttttt acgaggttct
     1741 tttcgccagt cctgtaattc catacttagg tgttcgtcta tgtcagatgg gcgtctaagt
     1801 taccaattct tgtctaatgg ctacatacgg tggtcggttt acgcttaccg caggacaggt
     1861 ggtaactgaa aaatagtttg ctgaggcgac tggaaacaga agggcaagat cgattccgac
     1921 agcagtgatg gtcagctacc tcatcgataa ctttgcaaca gtgcccttct tgaagcatca
     1981 tgcttcatga agaaaaactg gcacgtcatc aacgtaaaca ggcgatgtat acccgaatgg
     2041 tagccttccc ggcggtgaaa atgttcgaag agtatgactt cacgttcgcc accggagcac
     2101 cgcagaagca gctccagtcg ttacgctcac tcagcttcat agagcgcaat gaaaacatag
     2161 tgttacaggg gacatctgac atcacaaatc cccgggtggg gatttgtgta taagagacag
     2221 cccatggagg aaggggatat atcgcagctc tgacgactca ctccttattt acccgggaaa
     2281 tacttctcca gcaatgcata caggatggca gcggtttcag catccattat gccgtcataa
     2341 tttttctgcc ggaaatgaag ctgaaaagcg cggacaagcg ccttatatcc gtcatccgtc
     2401 tccacaccag aaatactgta tccataggtc ttcagccgtg acacgatatc aaccttatcc
     2461 ggaatgccct gcgattcaaa aaataaatat ttagtcagta attttatgtt tttcctgaaa
     2521 aaattactct ttttcgaact ccagtgagcg ttcacgactc atcagtgtga aaatgatggc
     2581 aaaaacagca gacagtatca gtacgataaa tgtggccccc catcccagat aatcgacaac
     2641 aagtcccatt cccagtgtcg caacaattga gccgaagata taggaaaatg agcctgacag
     2701 ccctaacaga ataagtttac gcgtaaatcg cccggaatcc agaagagcat taagtatcac
     2761 ataaacaggt tccataccag tcctgaccgc atcaggacgc cagtaaataa aatctcgtag
     2821 tccaccggca tcagagacaa gagtaatcgt cgttaccggt atttcgactg gtttgtactg
     2881 gtgtgagcgg acaaaactac tgtacgtctc tcatacacgc tcgtaagcgg ccctcacggc
     2941 ccgctaacgc ggagatacgc cccgactgcg ggtaaaccct tgtcgggacc actccgaccg
     3001 cgcacagagg cattttcatc gctgacaggc cgcatggcct cgcagagccg gagggggact
     3061 ggtgatattc ggacgaccgt accggcttgc gccgggcttc ggcggtttta cacctgtgct
     3121 acatgtaacc accgtatgcc gccttatatg cgctgactcg cattatttgc cttgttccgt
     3181 ctgaaagtaa tcactatgat taaatatgat taacaactaa tcgggggtga tgctgccaac
     3241 ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag
     3301 ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ctgccggaca
     3361 tcagcgc
//
LOCUS       DAETQN010000258         3308 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.263, whole genome shotgun sequence.
ACCESSION   DAETQN010000258 DAETQN010000000
VERSION     DAETQN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.263"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..110)
                     /locus_tag="K0W68_004450"
                     /pseudo
     CDS             complement(1..110)
                     /locus_tag="K0W68_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
     gene            complement(344..2053)
                     /locus_tag="K0W68_004451"
     CDS             complement(344..2053)
                     /locus_tag="K0W68_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HBI3075802.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2485..3204)
                     /locus_tag="K0W68_004452"
     CDS             complement(2485..3204)
                     /locus_tag="K0W68_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HBI3075803.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPNYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1023 a          635 c          542 g         1108 t
ORIGIN      
        1 tttttgttat aacaaaactc tcggaatcct tgatctttac tctttcttat aaaatcaata
       61 agatgcaacc caattccaag cctcatatct ttagtattaa atattggaac atactcaccc
      121 tctggatgtg acctttatcc atgaagaaga tttggccaca ccatggaaac ctgaacaatc
      181 atcagtttac gcccatgtaa acccctacgg gatatttgaa ctcgatatgg aaataaggtt
      241 gctaatcgag gtggttgcct gaaagttggc agttacccgg tcactacacc ttgttggagt
      301 gaacatatca aaaacaggta attacacaat ttaaattaca gtctcataaa aaatgattaa
      361 atctatttat tatttcaata aacgtttttt tcatcttctt tgaaaattgt tctgctatag
      421 tatccggttc cagattgttc tcttttatta ttctacaata ctctaatatt gtttctttgt
      481 ctctatttac tacagataca aatacaccag gaataccatc tttattagtt gcggacagca
      541 tttcgaccaa tcgtcttgtg gtaaaactat agttttttag aatattacaa aaggcacgta
      601 ttgtttcggt atggccatta taaaatgcca taaaaagtcc tgatgtaccg ttgggatttt
      661 ttatccataa taattcatct atatattcct ggtgaggggg gagtattttt agtatatctc
      721 catacgccat aatggccttt gtgtgcccat tttgaagtgc cataaacaac ccatgcttta
      781 ctttcaataa ttctttaatt tgttcactac taagcatttt cttattaagc agacttccga
      841 acatacgaat actatctgca tgtccattct gcaatgcaaa aaataaccca ggtaaatcat
      901 ggctgtcttt cgcagctaat agatataatc tcttatcgga agttaacggg gttttaaaga
      961 ctatttcccc ataggctttt attgtgtcta catgtccgtt cataaaagac atgcataaac
     1021 ccggcacatt gtttgcccct accgcagcaa gcaatgtaag tattgtttct tgactaagat
     1081 tgcacatatt taatatctta ccatatactt gaataatgtc tgaacatccg ttttgaagtg
     1141 ctatataaaa tgcaggagta ccatcagagc ttctcgtggc cagaaggttc accagtttag
     1201 attgagaaat ttcaccattt ttgtattttt ccatcagtat aaataatact tctgtcagat
     1261 tctttgttat cgcatgatat ataactctgg aattgatcaa ttcatctggt aaaagtttca
     1321 ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat tacctgcccg ggttgctcca
     1381 gaccataaat tttccaataa aaatcatcaa aaaaatcctt agcggataat tgactcagtt
     1441 cgaaatggct atcacaagta aatacagtac gtttatgagt aacagttcga ttaggatcga
     1501 aaaactgaac aacccatttt tcactttctt gtacgcgctt tattttcaac cctaacgcca
     1561 tttggtggtt taatgtgcat gctgataatg ttctgacaaa gtctccattt tttttcatgt
     1621 tataaaaaaa attagtaata actgttcccc actttttatt ttccacaaaa taaatataag
     1681 ctggtgattt tgaaaaatct tccgtttttt ccaaacatac attttttgca atagcatcag
     1741 gggtgctaag aagctgatag tttaccttcc catttgtttc cataaaattc atttcccaat
     1801 aggaagctag gtgacggcaa actatttgtc ttccatatat atcttttgcc actccattta
     1861 tatttacctt cacatttctc ttttctgaaa aataaggtgc tgttttattc ttaaactgaa
     1921 ttagttcact ggagtttgag accctcataa tagaacttgt tattgttcta ttatctctgt
     1981 taacagacat atctgttgat aacgaatttt ttgttgaaaa tatactagat gaaaatgttt
     2041 tatttaacgg catagaagac aaccatagag atgttataat actattagat tgcagttact
     2101 aatagtaaaa gcctccttac aaggaggtag cttattcaga atgctcctat aagatagcga
     2161 gctcgtcaac gatacagatg atctgattat attgtgcaaa aggagaacag gaaaataggc
     2221 actggaaaag atgaaggcct gatgggagca ttaactaatg caaaatccga caggttaata
     2281 tcccgaaaag caatcaaacg actcaaacag gagatgcacg ctgaagtggt ggacaactgt
     2341 tcggatttca gatgagtagc tcagcagttt atgagccgaa tgctctgtca cgggcaggaa
     2401 tcaggctgca tacgcagtgc caaatctaat cgtagttatt ctaaccagtt ttcaagctac
     2461 aaggtggtgt agctggcatc ttctctactc tatcatcaaa cgataaaatg gttcctctcg
     2521 taacatttgc tcttgccttt cacttccatc tcgatcgctt cgtagttcat tacgatagct
     2581 gacatatttc cagtcttcag gtctaacatc tgacgccgga tactccccgg gagcgatttt
     2641 gcttcttaat agattagact cgagacacat tataaattgt cgtattttat gaagtaataa
     2701 agcactatat tgcgagcatt cctgatctga ttttacatat agcgtaaact gagctcctat
     2761 ccccacacga gattgctgag aaactcgatt catatcagtt atcttccatt tatctatagg
     2821 actgtcctct gaaaataata aaccagacaa aatttggaat gccagaggaa cttgctccct
     2881 agctatacta atatgaaact tgtccccaac aaaatccccc ttactctgag gattttcacg
     2941 acatgcatga ataaacacat ctccactatg gttcacaatc tggaatcctt ggtacatgac
     3001 agaaaaaaat tgacatatat cagagtaatt tggcacgttc gatgctactt tgaaatagta
     3061 cgctgggtat tgccgtattt ggttgtacaa tatattgaag ttatttttca atctttcatt
     3121 cgcagaaagc atctgcggta tttcagacct cacaacattc aaagaatcta aatttagttt
     3181 tagacagggc ttttttatgg gcatagaaaa cgtcctctat aaaatagata tattatctta
     3241 tattgattgt ctatcatctt ttataaaacg atacattata tgtaatgtat gtaagcgtaa
     3301 gggtcttc
//
LOCUS       DAETQN010000259         3294 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.264, whole genome shotgun sequence.
ACCESSION   DAETQN010000259 DAETQN010000000
VERSION     DAETQN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.264"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(89..652)
                     /locus_tag="K0W68_004453"
     CDS             complement(89..652)
                     /locus_tag="K0W68_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBI3075804.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(756..974)
                     /locus_tag="K0W68_004454"
     CDS             complement(756..974)
                     /locus_tag="K0W68_004454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075805.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1133..1519)
                     /locus_tag="K0W68_004455"
     CDS             complement(1133..1519)
                     /locus_tag="K0W68_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBI3075806.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1509..1880)
                     /locus_tag="K0W68_004456"
     CDS             complement(1509..1880)
                     /locus_tag="K0W68_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBI3075807.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1880..2137)
                     /locus_tag="K0W68_004457"
     CDS             complement(1880..2137)
                     /locus_tag="K0W68_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075808.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2134..>3294)
                     /locus_tag="K0W68_004458"
     CDS             complement(2134..>3294)
                     /locus_tag="K0W68_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBI3075809.1"
                     /translation="ITDTGRKTWVKSSLEHYVAALRRNAALRDAEKTLNEALQKLRDA
                     HTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEVVERVECRNQGLEKSRTLMT
                     GIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKVTEQGHGVLLFTMEMANIQI
                     GERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTGRNIWMVDQANLTIDEICAT
                     TKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISKGLKGLAKSGGFPLIALSQL
                     SRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVYNPDTQATGIAEINITKQRN
                     GSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYSNTRTDSSKMERFF"
BASE COUNT          766 a          915 c          728 g          885 t
ORIGIN      
        1 catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat tcaacttttt
       61 acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc accagttgct
      121 ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt tccagagtac
      181 ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt ttatcgaagt
      241 ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt
      301 tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga tcggtttcga
      361 gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag cgaacgttac
      421 gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt aacgcatcac
      481 cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga ttgaaattgt
      541 ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt gtcatgacag
      601 gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc atagacatgt
      661 cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca aaaccgtcga
      721 aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg gtcataaacc
      781 aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc tttcgaggtg
      841 ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc
      901 caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac cggcgagctt
      961 ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg acaataaaaa
     1021 acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc cgacgccaga
     1081 ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc acctacataa
     1141 ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac atgccatcaa
     1201 taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac ccatgtttcc
     1261 gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa tcgtgcaaat
     1321 cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca tcatcatcag
     1381 aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca gcagcgatcg
     1441 acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt tccataacct
     1501 gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac gcccagtcgc
     1561 ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg
     1621 agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga
     1681 cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat
     1741 ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc accaactttt
     1801 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1861 aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca aactttccaa
     1921 ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt tgtgcgcgat
     1981 gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg
     2041 ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca
     2101 attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca tcttactgct
     2161 gtcggtacgt gtattgctgt atctgcgcgg ctggggctgc cgcattgggg tggaaagaac
     2221 ctgtgcgctt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt
     2281 ccccagagaa ccgttacgtt gtttcgtgat attaatttct gctatgcctg tagcctgtgt
     2341 atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat ccgcctctat
     2401 ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg actccacacc
     2461 gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca ggcctttaag
     2521 cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg tttttatcag
     2581 cccgagataa tcaaccacca ccagcgccgt ttccggatgt ttaatcagat ggtgtttcgt
     2641 tgttgcgcat atctcatcaa tggtcaggtt cgcctggtcc accatccaga tattgcgccc
     2701 ggtcatccgc cccacccctt gtgagaaacg cgcccagtct tcatcttcaa agtgagccac
     2761 agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt caccaatctg
     2821 gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag tcaccttgtc
     2881 gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa tgaataccag
     2941 gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt
     3001 cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt ccggaagcac
     3061 atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt
     3121 ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac gtaatttctg
     3181 cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc
     3241 aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag taat
//
LOCUS       DAETQN010000260         3222 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.265, whole genome shotgun sequence.
ACCESSION   DAETQN010000260 DAETQN010000000
VERSION     DAETQN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.265"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1680
                     /gene="tssI"
                     /locus_tag="K0W68_004459"
     CDS             <1..1680
                     /gene="tssI"
                     /locus_tag="K0W68_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBI3075810.1"
                     /translation="DFLCRMAAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPW
                     NPNTRTEVSTFCISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYE
                     VYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNR
                     EWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVV
                     TGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQE
                     VIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDAT
                     GKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVG
                     VNLVETVGSNQIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLH
                     KSLMVGMGYSVNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSI
                     FLNGTHIHLEGESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            1756..>3222
                     /locus_tag="K0W68_004460"
     CDS             1756..>3222
                     /locus_tag="K0W68_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBI3075811.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFD
                     IRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALW
                     QVLPEDVRLSPHVYLATNSLQGPWWILSWPEPPAYRVLTVVVDGFGRSLTFHRAAEGD
                     VAGAVTGVTDGAGRRFHMALSTQAQRAEASRKQRASSLSSPASPRSVSSSQVFPDTLP
                     AGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAVYDRSGTQVRGF
                     TYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITDSLN
                     RREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGK
                     LTSVVLPDGRTVRYGYNNQLQLTSVTYPDGLRSSRKYDR"
BASE COUNT          724 a          838 c         1071 g          589 t
ORIGIN      
        1 gatttcctgt gccggatggc ggcggaggaa ggcatcttct tttatgagga gcacgcgcaa
       61 aaaagtaccg accagagcct ggtcctgtgc gacaccgtgc gttatctgcc ggagtccttt
      121 gagatcccct ggaacccgaa cacccgtacc gaggtaagta ccttctgcat cagccagttc
      181 cgctacagcg cacaaatccg tccttcttcc gtggtgacca aagactacac ctttaaacga
      241 cccggctggg cagggcgttt tgatcaggaa ggccagtacc aggattacca gcgcacacag
      301 tatgaagtgt atgactaccc cggacgtttc aagggtgctc acgggcagaa ctttgcccgc
      361 tggcagatgg atggctggcg caacaacgca gaagtggcgc gcggaacaag ccgttctccg
      421 gagatatggc cgggacggcg aattgtgctg acggggcatc cgcaggcgaa cctgaaccgg
      481 gaatggcagg tggtggcaag tgaactgcac ggcgagcagc cgcaggcggt gccgggacgc
      541 agtggttcag gaaccacgct gaataaccac tttgcggtaa taccggcaga ccggacatgg
      601 cggccacagc cgttgctgaa accgctggtg gatggcccgc agagcgccgt cgtgacggga
      661 ccggcaggcg aggaaatctt ctgtgatgaa catggccgcg tgcgggtgaa atttaactgg
      721 gaccgctata acccgtcaaa ccaggacagt tcatgctgga tccgtgtggc acaggcgtgg
      781 gcaggcaccg gattcggtaa ccttgcgata ccgcgcgtgg gtcaggaggt cattgtggac
      841 ttcctcaacg gcgatccgga ccagccgatt attatggggc gtacctacca ccaggaaaac
      901 cgcacacccg gtagcctgcc ggggacgaag acgcagatga ccattcgttc gaaaacctat
      961 aagggcagcg ggtttaatga actgaagttt gacgatgcga cagggaaaga acaggtctac
     1021 atccacgtgc agaagaacat gaacaccgag gtgctgaata accgcaccac ggatgtgata
     1081 aacaaccatg ccgaaaaaat aggtaacaac caggcgatca ccgttaccaa taaccagatc
     1141 cagaacattg gcgttaatca gatacagacg gttggtgtca acctggtgga aacggtgggc
     1201 agtaaccaga ttatcaaagt gggatcaaac caggttgaaa aggtggggat cattcgtgcg
     1261 ctgacggtgg gtgtggctta ccagacgacg gtaggcggca ttatgaatac ctcggtggcg
     1321 ttgttgcagt cctcacaggt agggctgcat aaatcactga tggtgggaat gggctacagc
     1381 gtcaatgtag ggaataacgt caccttctcg gtgggcaaga cgatgaagga aaacaccgga
     1441 caaacagcag tttattctgc cggtgagcat cttgaactct gctgtggtaa ggcaaggctg
     1501 gtgctgacga aggacggaag catatttctc aacggtacgc acattcatct ggaaggggag
     1561 tcggatgtga acggtgatgc gccagtgatt aactggaact gtggtgccac acaacctgta
     1621 ccggatgcgc ctgtgccgaa agatttaccc cccggaatgc cggatatgcg gcaattttga
     1681 gtcaggtgca aaaaattaaa acggaactgg tgcagtgacc agatgaacca tattaaaagc
     1741 agaaggaatt gattcatggg cggcggaaaa ccggcggcgc ggcagggcga catgacccgc
     1801 aagggactgg atattgtgca gggttcagcg ggtgtgctga taggtgcgcc gacgggagtg
     1861 gcctgttcgg tgtgtccggg agggattacc tatgctaacc cggtgaaccc ggtgctgggt
     1921 gcgaaggtgc ttccgggcga gacggacctt gcgctgccct gcccgctgcc gtttattctt
     1981 ttccgcgcct acagcagcta ccggaccaga acacccgcgc cggtgggggt gtttggtccc
     2041 ggctggaaag cgcccttcga tatccgctta cagatacgcg atgaaggcct gatactcaac
     2101 gacagcggcg gcaggagcat tcactttgag ccgctgtttc ccggcgagat aagctacagc
     2161 cgcagcgagt cactctggct ggcgcggggc ggagtggcgg cgcagcacag ttcgcagccg
     2221 ctaagcgcgc tctggcaggt gctgccggaa gatgttcgcc tgagtccgca tgtgtacctg
     2281 gcgacaaaca gccttcaggg gccgtggtgg atactcagct ggccggagcc gcccgcatac
     2341 cgggtactga cggtcgtggt ggatggcttc gggcgctcac tgactttcca ccgggcggcg
     2401 gagggtgacg tggcgggcgc ggtgacgggg gtgacggacg gcgcgggccg ccgttttcac
     2461 atggcgctga gcacgcaggc gcagcgggcg gaagcgtccc gtaaacagcg cgcctcttcc
     2521 ttatcttccc cggccagtcc ccgttctgtt tcctcttctc aggttttccc cgacacgctg
     2581 cccgccggta cagagtacgg tgccgataac ggtatccggc tggaggcggt atggctgaca
     2641 cacgacccgg catacccgga tgaacagccg actgcgccgc tggcgcgcta cacgtacacg
     2701 gccggtggtg aactgcgggc ggtgtatgac cgcagcggga cgcaggttcg cgggtttact
     2761 tatgatgcgg agcacgccgg gcggatggtg gcgcaccatt atgcggggag gccggagagc
     2821 cgctaccggt atgatgatac cggtcgggtg acggagcagg tcaacccgga ggggctggac
     2881 taccgctttg agtacggaga gagccgggta atcatcacgg acagcctgaa ccggcgggag
     2941 gtgctgtaca cggaaggcga gggtggcctg aagcgggtgg tgaagaagga gcatgccgac
     3001 gggagcatca cccgcagcga gtatgatgag gcggggaggc tgaaggcgca gacggatgcg
     3061 gcggggcggc ggacggagta ccggctgcat atggcgtctg gaaagctgac ttcagtggta
     3121 ctgccggacg gcaggacggt acggtatggt tacaacaacc agttgcaact gacgtcagtg
     3181 acatatccgg atggtttacg cagcagccgg aaatatgacc gt
//
LOCUS       DAETQN010000261         3220 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.266, whole genome shotgun sequence.
ACCESSION   DAETQN010000261 DAETQN010000000
VERSION     DAETQN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.266"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            8..511
                     /locus_tag="K0W68_004461"
     CDS             8..511
                     /locus_tag="K0W68_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3075812.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(557..973)
                     /gene="rraB"
                     /locus_tag="K0W68_004462"
     CDS             complement(557..973)
                     /gene="rraB"
                     /locus_tag="K0W68_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBI3075813.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1135..2139
                     /gene="argF"
                     /locus_tag="K0W68_004463"
     CDS             1135..2139
                     /gene="argF"
                     /locus_tag="K0W68_004463"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBI3075814.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2211..>3220)
                     /locus_tag="K0W68_004464"
     CDS             complement(2211..>3220)
                     /locus_tag="K0W68_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBI3075815.1"
                     /translation="LLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDD
                     LNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPYI
                     NDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLFF
                     NLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSIL
                     VNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPGY
                     VVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKLNPPEDLRIAIKKLGWN
                     N"
BASE COUNT          893 a          719 c          675 g          933 t
ORIGIN      
        1 ttaaactatg aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgctgga
       61 taatcctgct atcgcccgcg tcattcgtca ggtatccgcc gaatacggtc ttaccgctga
      121 taaaggctac accgtcgccg atccgaatct tgacgagctg tatcaagtgt atagtcagcc
      181 tggccatgca tattgggtgg ttgagtacga gggtgaagtg gtcggcggcg gtgggattgc
      241 gccattaacc gggagtgagt cggacatttg cgaactgcaa aagatgtatt ttcttccggc
      301 tattcgcggt aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga
      361 gatgggcttc aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct
      421 ttatgagcat ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga
      481 ttgtgaagtg cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg
      541 cattttaatc aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat
      601 cgtcgccatc ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt
      661 caaatttctc cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca
      721 acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct
      781 cttctggatc ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag
      841 tttccagatc gtctgcggaa agatggtgtt cgatggtgta caacgcgtcc ggatcgctgc
      901 catcttccag taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt
      961 ccgggtttgc catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc
     1021 actgacattg cctccacctt tgtgataaag atttatgctt tagacttgca aatgaataat
     1081 catccatata aattgaattt taattcatta aggcgttagc cacaggaggg acctatgtcc
     1141 gggttttatc ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc
     1201 ctgctgcagt tagccgcgaa gctgaaagcc gataagaaaa gcggtaaaga agaagccaaa
     1261 ctcactggta aaaacatcgc gctcatcttc gaaaaagact cgacccgtac ccgatgctct
     1321 ttcgaagttg ccgcatatga ccagggggct cgtgttactt atctcggccc aagcggcagc
     1381 caaattggtc ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac
     1441 ggtattcagt atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc
     1501 gtgccggtat ggaacggcct gaccaatgag tttcacccca cgcagctgct ggcggatctc
     1561 ctcaccatgc aggagcattt gcccggcaaa acgttcaacg aaatgacgct agtctatgca
     1621 ggtgacgcac gtaacaacat gggcaattcg atgctcgaag ctgcagcgct taccggtctg
     1681 gatttgcgtc tggtcgcgcc acaggcatgc tggccggaag ctgcgctggt tacggaatgc
     1741 cgcgccctgg cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga
     1801 gttgaaggtg ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagag
     1861 aaatgggctg aacgcattgc attgctgcgt gattatcaag tgaacagcaa gatgatgcag
     1921 ttgaccggta acccggaggt caaattcctc cactgcctgc ccgcgtttca tgacgaccaa
     1981 acgacgcttg gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact
     2041 gatgaggtct tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat
     2101 accatcaaag cggtgatggt cgcgacgctc agtaaataat tgatatcgct ataaagatgc
     2161 cgggaaatac aaaaacatcc cggcgtcaca ttattttatg cagtgatagt tcaattattc
     2221 caccccaatt tttttattgc tatacgtaaa tcttccggag gatttaattt tcctaactgt
     2281 tcttcattaa tatatccata tatattgatt ttgccattcc ggaggatttt catcattctt
     2341 gttctaattt ttaattcatt acccatcttt tcttcaaaaa tcttaacgac ataacccgga
     2401 agtttattta caatttcttt tgttttttct tcatcaccat aaacatatcc taactcatta
     2461 agatacagct gttcgccctc ataacactta agtaattgat agaagtacca cttagcaaaa
     2521 tcaggttgct tcatgaaaaa atccttcaaa aaaatatcat ttactaaaat gctgacaggc
     2581 acttcattaa atagtaactt aacatcatca aataacctta gtattaagcg tgatttttct
     2641 tgattgctta agctaaaatc attgacatat gcaaccaact tatcccaaat caattcctgt
     2701 aatttacggc tatttttata gggttcttta tcgagattga aaaataaggt ctcagctatt
     2761 ttaattttag attgatcgca gctaaaatct ttagattgat cgcagctaaa atcaatatga
     2821 tttatcacgg agcatgcata ctttatcttt tcagaatcac caaaatattg cattctttta
     2881 ttaaaacacc aatccagtat tcttacatca ttaatatatg gggattttaa aaaagtctcc
     2941 agaatcgaat cgatgaccgg tgtaaggtta acttttgcat catctaagga atcaataaga
     3001 atatgagaca ggttaacttt gatgtcatta atttcattgt cagtagcttt cttattatta
     3061 agaagataat tatttactgc gttaaggtca tctatttctt tcagtatccc tttttcacca
     3121 agcaagtatg aaatcgattc attacataca taaggcatta atagcagatg ttctttcctt
     3181 attgctaata atgcattgtt caatggtttt tctaataaat
//
LOCUS       DAETQN010000262         3206 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.267, whole genome shotgun sequence.
ACCESSION   DAETQN010000262 DAETQN010000000
VERSION     DAETQN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.267"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(60..587)
                     /gene="mobB"
                     /locus_tag="K0W68_004465"
     CDS             complement(60..587)
                     /gene="mobB"
                     /locus_tag="K0W68_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBI3075816.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(569..1153)
                     /gene="mobA"
                     /locus_tag="K0W68_004466"
     CDS             complement(569..1153)
                     /gene="mobA"
                     /locus_tag="K0W68_004466"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBI3075817.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1223..1492
                     /locus_tag="K0W68_004467"
     CDS             1223..1492
                     /locus_tag="K0W68_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBI3075818.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1569..2555
                     /gene="srkA"
                     /locus_tag="K0W68_004468"
     CDS             1569..2555
                     /gene="srkA"
                     /locus_tag="K0W68_004468"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBI3075819.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2572..3198
                     /gene="dsbA"
                     /locus_tag="K0W68_004469"
     CDS             2572..3198
                     /gene="dsbA"
                     /locus_tag="K0W68_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBI3075820.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          820 a          773 c          778 g          835 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc ggatgcgaca ctggcgcgtc ttatccagcc tactcttgct
       61 tatccgttct gcttttgcat ccactccacc acaaaatcag ccagcccctc aacatcatta
      121 atatccagta atgcgacatc aagattaagc ggcacatcac tggctacagc aataacatgc
      181 ctgtctatca ctaattcttc aggtcgatgt ccggctccat cgcgaaacag cacaatcttt
      241 gcgatctctt catgcttaaa cccttcgacc agaatcaaat ccagctttga ggtatccatt
      301 cgacttgcga gaaactgtag atccagctct tcttcgtctg gtttttccgt catcaaggcc
      361 catcgttgct ggctggcaac gatggtttgt gccgcgccag ccttgcgcag ctcatagcta
      421 tctttgcctg gcttatcaac atccatatca tggtgcgtat gcttaatcag ccctggacgg
      481 atccctctgg cgcataatgc cgggatcagt tttttcaaaa gcgtagtttt tccggtacca
      541 ctccacgcgg caaaggcgag taacggtatc atcgtttttc ctgccatcgg gcaagctcct
      601 ctggcgtatt cacgttaaca aatgcatctt tatgatcgct gaaatcaacc gcatggccgc
      661 cagccagacg cataaatgcc attacccggc gttctcctgc ttgcagatat tctagtaata
      721 aaggctcaat agcacggttt accagagcaa tagtcgggtg atcgcgttca ccgtcatgga
      781 cccacacgac aggcgcatct ttgcgctgat gattaagccg ggaagctaaa acatggggaa
      841 tgtaaggcgt atcgcacgga caaaacaaaa accactcacc agcttcctgc tgcattactg
      901 aaagcattcc tgccagaggg cctgggtaat ccgccagtga atcttcaatc actttcagac
      961 cgcttgcctg atagatttcc tgatgacgat tggcattaac cacgacgtga gagagctgcg
     1021 tcataagcgc gtcagcgaca tgttgccata atggtttgcc gtttaattca agcaatcctt
     1081 tatctacgcc gcccattcgt ctggctttac cgcctgccag cacaacgcct gttatcgtcg
     1141 tcatcagatt caccgatatc gcctctttta ttgtgggatt gaccctgcta acgtgtctgt
     1201 ctcaagagta aggagcatca ctatgaaatg taaacgtctg aatgaagtta ttgaactcct
     1261 ccagccagcc tggcaaaaag agccagacct taacctgttg caatttttgc agaaactggc
     1321 gaaagagtca ggttttgacg gcgaactggc ggatttgacg gatgacattc tgatctatca
     1381 cctgaaaatg cgtgattccg cgaaagatgc ggtgatcccg ggtttgcaga aagattatga
     1441 agaagacttc aaaacggcgc tgttacgcgc tcgcggcgta attaaagagt aaaagcttgt
     1501 aagcggcgcc accaaaatca tcgtgaaatg atatccttca tcattcgtaa tgttttccgg
     1561 atgatgggat gaacaacagc gcttttactt tccagacact acacccggat accatcatgg
     1621 acgctctgtt tgagcaaggg atccgggtgg attccggtct taccccgctt aacagctatg
     1681 aaaaccgtgt ctatcaattt caggacgaag agcgtcgacg ttttgtcgtc aaattttatc
     1741 gccctgaacg ttggacagcc gatcaaatcc tcgaagaaca tcaatttgcg ttgcagctgg
     1801 taaatgatga agttccggtc gcagcacctg tggcctttaa cggtcagact ttattgaatc
     1861 atcagggatt ttatttcgct gtttttccaa gcgtcggtgg tcgccagttc gaagctgata
     1921 atatcgatca gacggaagcg gttgggcgtt atttagggcg tatgcaccag acggggcaca
     1981 aacagctttt tatccatcgc ccgaccatcg gtctgaatga atatctcatt gagccacgca
     2041 agctgtttga ggacgctaca ctgatacctt ccgggttgaa agcggcattc ctgaaagcga
     2101 cagatgagct gatcgccgcc gttacagcac actggcggga agatttcacc gttctgcggc
     2161 tacatggaga ctgccacgcc gggaatattc tctggcgcga tggtccaatg tttgttgatc
     2221 tggatgatgc acgtaatggt ccagctattc aggatttgtg gatgttgctc aatggcgata
     2281 aagccgagca gcggatgcaa ctggaaacta ttattgaagc ttatgaagaa tttggcgagt
     2341 tcgacaccgc tgaaatcgga ctgattgaac ctttacgcgc catgcgtttg gtttattatc
     2401 ttgcctggct aatgcggcgt tgggctgatc ccgcgttccc gaaaaatttc ccgtggttaa
     2461 ccggggaaga ttactggctg cgacaaacgg cgacttttat agaacaggca aaagttctac
     2521 aagaaccccc tttacaatta acacctatgt attaatcgga gagagtagat catgaaaaag
     2581 atttggctgg cgctggctgg tttagtttta gcgtttagcg catcggcggc gcagtatgaa
     2641 gatggtaaac agtacactac cctggaaaaa ccggtagctg gcgcgccgca agtgctggag
     2701 tttttctctt tcttctgtcc gcactgctat cagtttgaag aagttctgca tatttctgat
     2761 aatgtgaaga aaaaactgcc ggaaggcgtg aagatgacta aataccacgt caacttcatg
     2821 ggtggtgacc tgggcaaaga tctgactcag gcatgggctg tggcgatggc gctgggcgtg
     2881 gaagacaaag tcactgttcc gctgtttgaa ggcgtacaga aaacccagac cattcgttca
     2941 gcatctgata tccgcgatgt atttatcaac gcaggtatta aaggtgaaga gtacgacgcg
     3001 gcgtggaaca gcttcgtggt caaatctctg gtcgctcagc aggaaaaagc tgcagctgac
     3061 gtgcaattgc gtggcgttcc ggcgatgttt gttaacggta aatatcagct gaatccgcag
     3121 ggtatggata ccagcaatat ggatgttttt gttcagcagt atgctgatac agtgaaatat
     3181 ctgtccgaga aaaaataatt caatgt
//
LOCUS       DAETQN010000263         3142 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.268, whole genome shotgun sequence.
ACCESSION   DAETQN010000263 DAETQN010000000
VERSION     DAETQN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.268"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            61..576
                     /locus_tag="K0W68_004470"
                     /pseudo
     CDS             61..576
                     /locus_tag="K0W68_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            574..705
                     /locus_tag="K0W68_004471"
                     /pseudo
     CDS             574..705
                     /locus_tag="K0W68_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013183226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(799..1272)
                     /locus_tag="K0W68_004472"
                     /pseudo
     CDS             complement(799..1272)
                     /locus_tag="K0W68_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154977.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1445..2227
                     /locus_tag="K0W68_004473"
                     /pseudo
     CDS             1445..2227
                     /locus_tag="K0W68_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            2805..>3142
                     /locus_tag="K0W68_004474"
     CDS             2805..>3142
                     /locus_tag="K0W68_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBI3075821.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLT"
BASE COUNT          669 a          892 c          840 g          741 t
ORIGIN      
        1 ggcacggaac cacggcagct tgacggctgc aatgacgtgt gtcgtctggc ggtggagtat
       61 ctgctgtatg cagcccgaaa acgggggctg gaaatcggca tcttctgcac catccacact
      121 ttacgccttc actttgaaga acatttaccc ctcgttgtag ccggacgtcg cctgggcgta
      181 ccaaaatcaa cagtttgtag tatgtttgtg cgcttccgca aagctggcct ttcatggcct
      241 ctgcccgcag gtatgtcgga gcgggaactt gacgcccgtc tttacgggag tgcctccaca
      301 gtacctgtcg tacttactga aagtaccgtt atgcccgaag tccccggggt aaaaaagcgt
      361 ccccggcgcc ccaacttccc ttatgagttc aaaatcgcct tagtggagca gtcactgcag
      421 cccggagcct gtgtggcgca gattgcccgt gaaaatggaa tcaacgataa cctgctcttc
      481 aactggcgcc atcaataccg gaaaggtggc ctgctgcctt ccggaaaaaa tatgccggca
      541 ctgcttcccg tgacgttaac gccggagccg gataaccatg gttttgacat catctatatg
      601 ctttcgacac atcatcagcg gttcactttc gttcgtctct tcgatccata cctgatcggc
      661 tcacgtccga ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg
      721 gtggtttggt gcctgcgcct gcacgccgac accgaggggc cttccctcat ctcttgtgca
      781 gcatggctgc acagggactc acgtccctgg tgcgcgcctt cgtggcacac catcatcggc
      841 gtacctttcg aagggaacgt cgggacactg acgttgcatc cacatatcaa aggcatagtg
      901 caggaacaga tttgagagca ggggcgagat cacgccgccc tgcggcgttc cttctgtcac
      961 cggccttacc agacctgcgt ccattacccc tgcattcagg aacctgcgga taagtgacaa
     1021 cacccgttta tccgatactc gtttctctat ccggctcatc agcacgtcgt gatttatccg
     1081 gtcgaagaac ttctccagat cgaggtcgac cacccagtga tacccggccc cgatatattc
     1141 ctgggcctgt atcactgcct ggtgggccga acgaccggga cggaacccgt agctgttgtc
     1201 actgaacgag ctatctcaca gtgcctgcaa tacctgcatc atagcctgct ggatgaagcg
     1261 atccaccacc atctggatgc ccagcagacg ttcgccgccg ccgggcttca gtatggatac
     1321 tcttctcaca ggggatgggc ggtatctgcc ggacagcagt tgcgctttca gctctggcca
     1381 gtggtgcctc aggtagtccg gcagttcact tacgcgcatg ccatcgactc ccgccgcacc
     1441 ttgattgcgt cttgtcgatg gaacagcaat cagcgcgccc gggggcggca gcgctgaatg
     1501 gcgactacat atgggatatg atcctcatac ctgtcagttc actgattttg agctaaccga
     1561 cagcagagac gctgaacggc tggatcgatt tgcgcaaacg gcagacgaga tacgaattgc
     1621 tgaccgggga ttcggttcgc gtcccgaatg tatccgctca cttgcttttg gagaagctga
     1681 ttatatcgtc cgggtttact ggcgaggatt gcgctggtta actgcagaag gaatgcgctt
     1741 tgacatgatg gattttctgc gcgggctgga ttgcggtaag aacggtgaaa ccactgtaat
     1801 gataggcaat tcaggtaata aaaaagccgg agctcccttt cctgcacgtc tcattgccgt
     1861 atcacttcct cccgaaaaag cattaatcag taaaacccga ctgctcagcg agaatcgtcg
     1921 aaaaggacga gtagttcagg cggaaacgct ggaagcagcg ggccatgtgc tattgctaac
     1981 atcattatcg gaagatgaat attcagcaga gcaagtggct gattgttacc gtctgcgatg
     2041 gcaaattgaa ctggctttta agcggctcaa aagtttgctg cacttggatg ctttgcgtgc
     2101 aaaggaactt gaactcgcga aagcgtggat atttgctaat ctactcgccg catttttaat
     2161 tgacgacata atccagccat cgctggattt tccccccaga agtgccggat ccgaaaagaa
     2221 gaactaacgc gttgtggaga ataacaaaaa tggtcatctg gagcttacag gtggccattc
     2281 gtgggacagt atccctgaca gcctacaaaa cgcaattgaa gaacgcgagg catcgtctta
     2341 acgaggcacc gaggcgtcgc gttcttcaga tggttcaacc cttaagttag cgcttatggg
     2401 gtaaatcccc gcctttttcc tgccggtccc cggacagagc acaaaggaag tcgccgccgg
     2461 ctccgcttta cccggccatg cggggcatgg ccttgtgggt tttcagctct gtagcctcag
     2521 cgtcgtgtgc gggctgtgcc gtgcctccat cttagccggg ctggcgttga tgcaagggta
     2581 cgcttcgccg ctgcggtcac ccggtccctc cttcccgtct gccgtgattt tccggtcgtt
     2641 cacccgctca gatttcgcgg tttcaccctc caactcccgc cagccgtgtg cgtcaggctg
     2701 cggcttccct tgcgccctgt gcatccccgc cttatcgccc ggctaagatg gaggcacggc
     2761 accgcccggt gtcgcggaat atgtgaacaa cggaggaaaa catcatgcaa tatgcgaaac
     2821 ctgtcactct gaatgttgaa gagtgcgacc gtctctcctt tctgccttac ctgtttggca
     2881 acgattttct gtatgccgag gcgtatgtgt acgcgctggc gcaaaaaatg atgccggaat
     2941 atcagggcgg cttctggcac ttcatccgcc tgccggacgg tggcggttac atgatgccgg
     3001 atggcgaccg tttccacatg gtgaacggtg caaactggtt tgaccgtacc gtgagtgctg
     3061 atgccgcagg catcattctg acttctcttg tgattaaccg ccagttgtgg ctgtaccacg
     3121 acagcgggga tgcaggactg ac
//
LOCUS       DAETQN010000264         3112 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.269, whole genome shotgun sequence.
ACCESSION   DAETQN010000264 DAETQN010000000
VERSION     DAETQN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.269"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1258)
                     /locus_tag="K0W68_004475"
     CDS             complement(<1..1258)
                     /locus_tag="K0W68_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBI3075822.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLIERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVFPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLLN"
     gene            complement(1415..1624)
                     /locus_tag="K0W68_004476"
     CDS             complement(1415..1624)
                     /locus_tag="K0W68_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075823.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1647..2357)
                     /locus_tag="K0W68_004477"
     CDS             complement(1647..2357)
                     /locus_tag="K0W68_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3075824.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2873..>3112)
                     /locus_tag="K0W68_004478"
     CDS             complement(2873..>3112)
                     /locus_tag="K0W68_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075825.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          913 a          653 c          507 g         1039 t
ORIGIN      
        1 aattaagtaa attagtcagt gaccttgtaa agtctgttaa accttcctgt gaaccatcga
       61 acattaaggc cagattatta ttccatgaat tacttaagcg gcctaactgc gtctgagtag
      121 agttaataat cttttcccat ccaccagctt tatcaatcat ccttcccatt tcagcggata
      181 ctttaggaaa aacatcttta gaaagaacct taccgtcttt catggccttg tctaattctt
      241 ccgtagtcat cccaagactt ctggcaaata actgaaaagc acctgccatt ctgtcaccta
      301 attgaccgcg tagctcttca gcctgaatct ttcctttaga tgccatctgc tcgaaagctt
      361 ttaaaatccc gttaatttca tcggtgctta gccccattaa tctggatgta ccaatcacat
      421 tagaaaataa ggattctttc tggtctgcat tcatatcacc agcaccagcg gaaaacttag
      481 caaaactgct taatgtttca atctggtcta caccactact atcagcaaga ttcttaacaa
      541 attctgtagc tcgtttagca ccttcatcac ctaaaacgaa ttgtgccgct cgttgtgatg
      601 cttctttttt cagaccaacc tccataatcc gcctgtaagc ttctaaggct gtctgaaaac
      661 tataaatggc tgtacctgct ttgattgcac tcgtagctaa tacatccaga ctatttgaag
      721 cattttcact gtgattattt ttgctggtgg tcttgttaaa cttagaaagt gtttcacgaa
      781 gagactgatt taactttgct aactcacgat taaaagtttc gtttccaagt gtcttggatt
      841 tactgcttaa gctgtaatac tggcttttaa aatacttctc aagcatagga ttaaagtcac
      901 gaagcttgct catattcgct gagtaagctg ctgtgattgc tctgcttccg ctaattcgtg
      961 gctgtcttgg ttgtctaagt ggctttaaag tatcctcaat ttcagtttta atcttaaacg
     1021 ctctagtgtt tttaagacgc tgctcaatct tggaaacagt tttatcgaca tttctttcta
     1081 ttagtttgag agaatcattt aaaggcgtag gattaactct aaacgactta ttcaatcgct
     1141 tctcaattcg tgatgctacc tgcatcgttt gtttctcggc tttttccaag ccttttaaaa
     1201 ctttctgaga gttaaaactt aactcaatca gaaaatcatc aactttaaat tgtgccattc
     1261 atatttttca cctctttaag cgtgttgtac gtaaattcta tttttcagtg tgggtattag
     1321 ctttacagat ttcacgtagc acacatacaa aaaaagggag agctaccgat gtcactcgat
     1381 aatctctccc cgtatttacc caaccaattc ttttttaatg tcggacaaga taatgtaatt
     1441 ccgtttcacc tatgatttga gcatcagaag gaatacggtc aatacaccac tttggaatct
     1501 tgcgtgtttt tggtttgtgt gaggtgataa cttgacgttt gtttttaatt cttaaggctg
     1561 gcttagtttg taactttaag ttttcacaaa cagaccgaat agtctctaaa cgttcttcaa
     1621 acattaatca cctctttaat tcataatcaa acagccttgc tatacttctg agaaatcttc
     1681 gttaactgct caatatcagc catcaaagca atcttcttct gcgactcata ttcactaaca
     1741 agttctttca tagtatcaag ttctttttta agcttttgct ttaacttatc cagtttaccg
     1801 gaataatgct tagtcaaatt agcttcagct aactgaagaa tctcttcatc tgttttatca
     1861 gtcatcagaa taaattgacc agaacccctt aatgcttgtc tgacatcaat taaccggaac
     1921 ccttcaggga tactttgaag aactttagga acgtcttcaa tcgctgtacc aaaaccatca
     1981 ttaaagtttc cttcataata gataaacggg tttgacttaa tctcgtaagc aatatcatat
     2041 tctgaaggtt tcgaggtttc ttcgaagttc ttaacagcat cgtaagcgac atcaagtcta
     2101 agacgctgtg attttaaagc tgggtaatat tcacgctcaa gctcaaatag tgcttcagtt
     2161 ttgcagatat caggcatttc ttgaggctca ggagacacaa aggaaattac ctttggtgac
     2221 atctttagct cttgctcaag ctcaatgcgc ttgtcatgct cttgacgctg aatagtatgt
     2281 agcggatgtt caccctgtgg tgaccagtct ccgaagctat caaccttttt accgttaata
     2341 tataaatcgt ttgtcattat aatttcctct aagtgtggtt atttcttaat agagcacctt
     2401 caacgccaat acacttagaa gatgacttaa cgatttatta aagatgctct attaagaaaa
     2461 gccctaaaca taatctgtga agggctttgg aggtttgctt ataacgttaa cgaagtttca
     2521 ggaaaacttt ctttaaaagc tctgtttaaa aactcttaaa gaaagcccta actcatgatt
     2581 agggcaaagc tgtaaaggat tcaataatga atgaacaaag actttttgag atagcctctc
     2641 agaccaacct gaaaggctag gacgaaaagc attgcacctt tccaactcag aatatccatt
     2701 catcggatta ttgaaagaaa aaataagaga gaattgctct cccccatata tacaattatg
     2761 tcatgctcaa ggttgtttgt caacagcctt taacagattg gtaacattta tactttaata
     2821 gatgcacctg caatcatacc aatcaataca aaacagataa tcaccaattc cattaatgca
     2881 ccaccttcga atccgtcacc agaatattat cgctatacaa aattgctata gccgccttga
     2941 gatatttttc agggctaacg ctgtatttgt tcgtttcttc gataataaga cgcttcatgt
     3001 ttgagtcggt aacaggaaac ataaaacgcg gttcaccagt ttcaatttcc tgaataaagt
     3061 cttcaatttc ttctgagttt tcagccatta gctttagtaa tttatcaagg tc
//
LOCUS       DAETQN010000265         3046 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.270, whole genome shotgun sequence.
ACCESSION   DAETQN010000265 DAETQN010000000
VERSION     DAETQN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.270"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..173
                     /locus_tag="K0W68_004479"
     CDS             <1..173
                     /locus_tag="K0W68_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075826.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            186..1037
                     /gene="hyi"
                     /locus_tag="K0W68_004480"
     CDS             186..1037
                     /gene="hyi"
                     /locus_tag="K0W68_004480"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBI3075827.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            956..2134
                     /gene="allB"
                     /locus_tag="K0W68_004481"
                     /pseudo
     CDS             956..2134
                     /gene="allB"
                     /locus_tag="K0W68_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2191..>3046
                     /locus_tag="K0W68_004482"
     CDS             2191..>3046
                     /locus_tag="K0W68_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HBI3075828.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          697 a          710 c          836 g          803 t
ORIGIN      
        1 aggcgaaagc cttaatggcg caatatcggg taccggtagt cgtggaagtt attcttgagc
       61 gtgtgaccaa tatttcgatg ggcagcgaac tggataacgt catggaattt gaagatatcg
      121 ccgataacgc agtggacgca ccgactgaaa cctgcttcat gcactatgaa taagggagat
      181 aaataatgtt acgtttctct gctaatttat cgatgttatt tggtgaatat gattttctcg
      241 cccgttttga gaaagctgcg cagtgtggtt ttcgcggcgt tgaatttatg tttccttatg
      301 actacgacat tgaagaatta aaacaggtgc tggcgagtaa taaactcgaa catacgctgc
      361 acaatctacc ggcgggtgac tgggcggcgg gggagcgcgg tattgcctgt attcctggcc
      421 gtgaagaaga gtttcgggat ggcgtagcag cagcgattcg ttatgcccgt gcgctgggga
      481 ataaaaaaat taactgtctg gtcggtaaaa cgccggctgg tttcagcagt gaacagattc
      541 acgcaacgct tgtagaaaac ctgcgttatg ccgcgaatat gctgatgaaa gaagatattt
      601 tattactgat tgaacctatt aaccattttg atattcctgg tttccatctc accggaactc
      661 ggcaggcgct gaaattgatt gatgatgttg gttgctgcaa tttgaaaatt cagtatgaca
      721 tttatcacat gcagcggatg gaaggtgaat taaccaacac catgactcag tgggccgata
      781 aaattggtca cctgcaaatt gctgataatc cgcatcgcgg cgaaccgggg accggagaaa
      841 ttaattatga ttatctcttt aaggtaatcg aaaattctga ctacaacggt tgggttgggt
      901 gtgaatataa accccaaacc accacggaag ccggtttacg ctggatggat ccgtactttc
      961 tgaaccgggt cgtagccact gggaaggtta tgaaaccggt actcgcgcag cggcaaaagg
     1021 tggtatcacc accatgatcg aaatgccgct caaccagctg cctgcaacgg ttgaccgcgc
     1081 ttcaattgaa ctgaagttcg atgccgctaa aggcaagctg actatcgatg cggcacaact
     1141 cggtggcctg gtgtcttaca acatcgaccg tctgcatgag ctggatgaag tgggcgttgt
     1201 cggcttcaaa tgcttcgttg cgacctgtgg cgatcgcggt atcgacaacg acttccgtga
     1261 tgtaaacgac tggcagttct tcaaaggtgc gcagaagctg ggcgaactgg ggcagccggt
     1321 gttggtgcac tgcgaaaacg cgctgatctg tgacgcaccg ggcgaagaag cgaaacgtga
     1381 aggtcgcgta actgcccatg actatgtggc ttcgcgtccg gtatttaccg aagtggaagc
     1441 gattcgccgc gtgctgtatc tggcgaaagt tgccggttgc cgtctgcacg tttgccatat
     1501 cagcagcccg gaaagtgttg aagaagtgac tcgtgcacgt caggaaggtc aggatgttac
     1561 ctgtgaatcc tgcccgcatt actttgtact ggataccgat cagttcgaag aaatcggtac
     1621 tctggcgaag tgttcaccgc cgatccgcga tctggaaaac cagaaaggca tgtgggaaaa
     1681 actgtttaac ggtgaaatcg actgcctggt ttccgaccac tcaccatgcc ctccggaaat
     1741 gaaagccggc aacatcatgg aagcatgggg cggtattgcc ggtctgcaaa actgtatgga
     1801 cgtgatgttc gatgaagcgg tacagaaacg cggaatgtct ctgccaatgt tcggcaaatt
     1861 aatggcgact aacgcagcag atattttcgg tctgcagcaa aaaggccgta tcgccccagg
     1921 aaaagatgcc gacttcgtct tcattcagcc gaatagcagc tatgttctta ccaatgacga
     1981 tctggaatat cgccacaaag tcagcccgta tgttggccgt accattggcg cgcgtatcac
     2041 gaaaaccatc ttacgtggtg atgtgattta cgacattgaa cagggcttcc ctgttgcgcc
     2101 gaaaggtcaa tttatcctta aacatcagca gtaatctggc ccctgcaatg cccgtccttg
     2161 cggcgggcat tctccggtta aggtgtgttt atgttcaatt ttgcagtcag ccgcgaaagc
     2221 ctgttatcag gatttcagtg gtttttcttt attttttgca acacggttgt ggttcctcct
     2281 acgctacttt ctgcttttca gttgccgcaa agtagcctgc ttacgctcac gcaatatgct
     2341 tttcttgcta ccgcactggc ctgcttcgct caggcgttct gcggtcatcg tcgcgctatt
     2401 atggaagggc cgggtggcct gtggtgggga accatcctta ctatcaccct tggagaagca
     2461 tcgcgcggga caccgatcaa cgatatcgct accagtctgg cggtggggat tgcactctcc
     2521 ggcgtgctga cgatgttgat tggttttagc ggattaggcc atcgcctggc acggttattt
     2581 acgccgtcgg tgatggtctt gtttatgttg atgctgggcg cacagctgac cactatcttt
     2641 ttcaaaggta tgctcgggct gccgtttggc atagccgacc catattttaa aattcagtta
     2701 ccaccgttcg cgctctcggt ggcggtgatg tgcctggtac tggcgatgat tatctttctg
     2761 ccgcaacgtt ttgcccgtta tggcctgctg gtcggcacca taaccggctg gttgttgtgg
     2821 tacttttgct ttccttcttc gcattcgctc tccggtgagt tgcactggca gtggttcccg
     2881 ctcggcagtg gcggtgcttt gtcgccggga attattctga cggcggtgat tacaggtctg
     2941 gtaaatatca gcaataccta cggtgcgatt cggggcacgg atgtttttta tccgcagcag
     3001 ggcgcgggga atacgcgtta tcgtcgcagc tttgtggcga ccggat
//
LOCUS       DAETQN010000266         3010 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.271, whole genome shotgun sequence.
ACCESSION   DAETQN010000266 DAETQN010000000
VERSION     DAETQN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.271"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            63..1226
                     /locus_tag="K0W68_004483"
     CDS             63..1226
                     /locus_tag="K0W68_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBI3075829.1"
                     /translation="MPNHDRFECQTAIDSDAILQLAWAVFKIFRIPVNQWTLATAALG
                     GVFLVSGLILLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFK
                     LDPVRYQARVDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKG
                     SQAAVNPFSERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQ
                     LTEAKYNLEQTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQN
                     SLLRLKPGDDAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVL
                     GTIELDPNDDIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1686..2123)
                     /locus_tag="K0W68_004484"
     CDS             complement(1686..2123)
                     /locus_tag="K0W68_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075830.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(2212..>3010)
                     /locus_tag="K0W68_004485"
     CDS             complement(2212..>3010)
                     /locus_tag="K0W68_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBI3075831.1"
                     /translation="QMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYD
                     EWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNN
                     MQCKNNPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLT
                     QSCLNAFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          792 a          649 c          676 g          893 t
ORIGIN      
        1 tcaatgatcg gaagtatgcc aaatcgtgat cgattgtgat gccaaactgt gatcggagat
       61 ggatgccaaa tcatgatcga ttcgaatgcc aaactgcgat cgattcagat gccattttgc
      121 agctggcgtg ggcggtcttt aaaatctttc gtattccggt gaatcagtgg acgctggcga
      181 cggcggcgct gggaggcgtg tttctggtga gtggtttgat tttgttgatg aactacaacc
      241 acccttacac ttttaccgcg caaaaggcag tgatagcgat ccctatcacg ccacaggtga
      301 cgggaattgt tactgaagtc actgacaaga ataatcagct tattcaaaag ggcgaggtgc
      361 tttttaagct cgacccggtt cgttaccagg cgcgagttga cagacttcag gctgacctga
      421 tgacggcgac gcataatata aagacgctgc gtgcgcagct cactgaagcg caggccaaca
      481 ccacccaggt ttcagcggag cgcgaccgtc tgtttaaaaa ttatcaacgt tacctgaaag
      541 gcagccaggc ggcggtgaat ccgttctcgg aacgtgacat cgacgatgcg cggcaaaatt
      601 tcctcgcgca ggatgcgctg gtgaaaggct cggtggcgga gcaggcgcag atccagagcc
      661 agctcgacag tatggttaac ggcgagcaat cgcagattgt gagcttaaga gcgcaactta
      721 ctgaagcaaa atataacctt gagcagactg tcattcgcgc gccgagcaat ggctacgtta
      781 ctcaggtact gatccgccca ggtacatacg cagctgcctt gccgctgcgt ccggtgatgg
      841 tcttcatccc cgagcaaaaa cggcaaattg tcgcccaatt tcggcaaaac tcgctgttac
      901 gtctgaaacc tggcgatgat gcggaagtgg tgtttaacgc gctacctggg caggtgttcc
      961 acggcaaatt gactagtatt ttacctgtcg tgccaggcgg ttcttatcag gcgcaggggg
     1021 tattgcaatc attaacggtc gttcccggca cggacggcgt gctgggaacc attgaactgg
     1081 accctaacga tgatatcgat gccttacccg acggcattta cgcccaggta gcagtttact
     1141 ccgaccattt cagccatgtt tcggtgatgc ggaaggtgct gctaagaatg accagttgga
     1201 tgcattattt gtatttggat cattgagggg gaagggggag attatggaac tttgctccct
     1261 gcaagggcta attatttttg catattactt cattggctgc gatgattttt ctttaatatt
     1321 cattatgtta ataagttctg gatgagtgtg ctctcattgg attttttatc cgtcttgttc
     1381 tgccgcagga tgagactata catcgtcgag atcgcaatga tgatatgagt tgttcctcct
     1441 gcgacggctc taatcaaaaa agagcaggag tgcttttcca tctattgcga tgatggcttt
     1501 attatctgaa ggatgacagt cacatttaaa aaatatttaa tctatagatc acaatgtagg
     1561 gtcatggaat atatgtatac aactattttc ttcttcactt ttaattatcg tattcattat
     1621 aaaactaagt taaaaaaact cctagagagt gagttgttaa tatattttta aataaaatta
     1681 agttatcatt catcgatgca ttttatagaa tcatcaatgg ccataatagt actattaact
     1741 ctcaatttta tatttttatc atcactacaa atattaactg aatttatatc aatgattttt
     1801 ttattcttat cttttatttt atttactgtt tcaatgcaat cagcggcaag ttggagtgga
     1861 tttagatttt tcagatatgg tcttatgatc tttcccttac ttaataaata gcatgtatag
     1921 gattcggaaa gtccagcatc tattttgtac ctcattatat tagataagga tgtgagaaat
     1981 tccttgttat cattcgcagc catatatgcc agtccgagtt cagcctctgc ggccttacag
     2041 gcaaaatagc ttagtgaaca cgcttttcgt ccctctttag tactggctga tattagtgcc
     2101 cataatattg ctttgtctgc caaatctaac tctctctgaa attgtcttgc agtaactgca
     2161 ctcgcagaaa tagagaaaaa aatacaattg ttaataactt tgccatattt tttagtccgt
     2221 aactgttaat gtgctatctg gctctgaaaa tattaactca tttaattttt gcatgtcatt
     2281 agatgaacca gttatacaac cctcggaaca gaatcgaggt cctaaagacg gaccgaaagc
     2341 gtttaaacaa ctttgtgtta aaaatccatc acgattatat gtttctgtat ttgctgacgg
     2401 tactaaacgt ataccattag gttttctatt ggttgctcca ggaccattaa cattccagct
     2461 ccatacccct tgaggtattg gacctctatt ttgtaagtgt gtacaaccag gattattttt
     2521 acattgcata ttattaccat tattaccaga ggcgacagga atagtaagca catcataaga
     2581 gttatcatca ttttgcgtat ctgaaacgca tgaaagcata cttaaagtta tgctgtataa
     2641 acaggtcgct aatcccaacg ggtcaatatc agttatcgga ttcaacggat actgataaaa
     2701 attccatccc cccttcagtc caatcggatc ctgagtgata tatcgcccct gcagcgggtc
     2761 ataatagcgg tggcggttgt aatacaggcc ggactcctca tcatactgct gccccggcag
     2821 gcggataagc tgctgcagct gatgcgggtt ctcttcattc agcaggttgc cccactcatc
     2881 gtattctgcg caccattctg ttgccccttc cgtgctgata agcaccagcg gcaggccgcg
     2941 atggtcgcag tggtacaggt ggatttttcg cgccggcgtg tacaccgggt ccatctggtt
     3001 ttgcatctgc
//
LOCUS       DAETQN010000267         2994 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.272, whole genome shotgun sequence.
ACCESSION   DAETQN010000267 DAETQN010000000
VERSION     DAETQN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.272"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            18..1076
                     /locus_tag="K0W68_004486"
     CDS             18..1076
                     /locus_tag="K0W68_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBI3075832.1"
                     /translation="MAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTV
                     FTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACH
                     SLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFR
                     DHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKS
                     SEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDH
                     LVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKK
                     SFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="K0W68_004487"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="K0W68_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBI3075833.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2994
                     /locus_tag="K0W68_004488"
     CDS             2805..>2994
                     /locus_tag="K0W68_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylanase"
                     /protein_id="HBI3075834.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSL"
BASE COUNT          839 a          632 c          745 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc ttag
//
LOCUS       DAETQN010000268         2965 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.273, whole genome shotgun sequence.
ACCESSION   DAETQN010000268 DAETQN010000000
VERSION     DAETQN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.273"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..216
                     /locus_tag="K0W68_004489"
     CDS             <1..216
                     /locus_tag="K0W68_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBI3075835.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(271..369)
                     /locus_tag="K0W68_004490"
                     /pseudo
     CDS             complement(271..369)
                     /locus_tag="K0W68_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(415..597)
                     /locus_tag="K0W68_004491"
     CDS             complement(415..597)
                     /locus_tag="K0W68_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3075836.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
     gene            696..1586
                     /locus_tag="K0W68_004492"
                     /pseudo
     CDS             696..1586
                     /locus_tag="K0W68_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1589..2122
                     /locus_tag="K0W68_004493"
     CDS             1589..2122
                     /locus_tag="K0W68_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBI3075837.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2151..2678)
                     /locus_tag="K0W68_004494"
     CDS             complement(2151..2678)
                     /locus_tag="K0W68_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBI3075838.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2680..>2965)
                     /locus_tag="K0W68_004495"
     CDS             complement(2680..>2965)
                     /locus_tag="K0W68_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3075839.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          837 a          661 c          649 g          818 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccag ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg taggccactc
      301 aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact ttttctaggc
      361 ttccaccacc agcacgacaa gatgccgcat acagtgcccc agtcagtcca gttttcagac
      421 aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag gtcggcctgt
      481 ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa caagagcgtt
      541 tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa tcagcacgct
      601 gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgcc attatctaaa
      661 acctcggttt acgagaactc ggcacaagcg gggagaaaat accgttactc agtacagcga
      721 atacctggac taatcgacaa acattcagcg gtggcctttc tggtgaacta tccggcaatg
      781 cttctacagc tgcaaaatta aaaactgcca ggaaaataag caatgtggct tttgatggtt
      841 cctccgatat cacattaaaa gcaagtcatg ttggtgcgtt tgccttaggg aaaacaggaa
      901 gcaccgttgc gaatgataaa gcagttggat ggaactggag tagcggagcc tataacgcaa
      961 ctattagtgg tgcatcaacg ttaattattc atttttatat gggagaagga agttgtcctg
     1021 cagctcagtt tcggattaat tataaaaatg gcggtatttt ttatcgttca gcccgtgatg
     1081 gttatggttt tgaagccgac tggtccgaat tttacaccac cagaaaacct tcagcaggag
     1141 atgttggtgc actgccgtta tctggtggtc aactgaatgg tgcactgggt atcggaacat
     1201 ccagtgctct tggcggtaat tcgattgtat tgggtgataa tgacacgggc tttaaacaaa
     1261 aaggcgatgg taatctggat gtttatgcta ataacgtcca tgttatgcgc tttgtctccg
     1321 gaagcattca aagtaataaa accataaata ttacgggacg tgttaatccc tcggattacg
     1381 gtaactttga ttcccgctat gtgagagatg tcagacttgg cacacgagtt gtttagacca
     1441 tgaagaaagg cgtgatgtat gaaaaatcag gccatgtaat cacggggctt ggtattatcg
     1501 gtgaagtcga tggtgatgat ccggcagtat tcagaccaat acaaaagtta attaacggaa
     1561 catggtataa cgtatcgcag gtataatcat gcagcattta aaaaacattg tcgcaggtaa
     1621 tccaaaaacc gttgaacaat accagctaac aaagaatttt gatgttatct ggttatggtc
     1681 cgaagatgga aaaaattggc atgaggaatt aaaaaacttt caggaggata caataaaact
     1741 ggcttacaca gttgagggaa taattattgc tatagacaag gatgtatcag caattaatcc
     1801 agaaggttta agtgtcgttg agttgcctga tattacagca aatcgccggg ctgatatttc
     1861 gggcaactgg atgttcaaag atggtgcggt gataaagcga acttataccg aggaagagca
     1921 aaggcaacaa gcggaaaatg aaaagcagag cctgctgcag ctcgtcaggg ataaaaccca
     1981 gctgtgggac tctcagctac agctgggcat catttccgat gagaataaac aaaaattaac
     2041 cgagtggatg ctctatgcgc agaaggttga atccacagac acctccagcc tgccagtaac
     2101 gtttcccgaa caaccagaat gagagaaggc ccgctatcgg gccttaattt ttattcaggc
     2161 ttttgtggcc attcaggatt tgccgtatcc acacggctga ccagaacact gtagcgttcc
     2221 catgcttcca gtcgtgtgcg ttcctcgtct gttgccatat tcagcctgac agctcgttcc
     2281 agcggctgga tgactgattc agcttcggaa agcaatgcgg ccttttgtga ttcggcctgt
     2341 tgttgcagtt cctgttctgt atattccctc tttactactt taccatccag aaacatccat
     2401 tttccgttat tgtctgcgtg acgatttgcc gtaatatcag gaacttcaac tatgcttagc
     2461 ccttcaggat taatagttga aacgtcttta ctgacagcaa cgatgatgtt gttctcatcg
     2521 taagcaattt ttatggtgtc tggctgaaaa ttcttttgtt cctcatacca gtttttgcca
     2581 tcttcgcaaa agagccatac cacgcccgct tttttagtta gctggtattg ttccggtgtt
     2641 ttcggattcc cggccttaat atttttcagg tgcatcatct taaacactcc ccacgttgta
     2701 ccatattccc ccaatcaatt tctgaattgg ccttctggca acagtgtcga caatatcatc
     2761 cgcattgctg ttaattgcgg ctgttaaaac ataacctgac gcatcgccaa agccgtagtc
     2821 gcgccatacg tttttatatt caatgctgcc aagacgaata tcccgaacga catgattatg
     2881 cagccatgtg cttaaccagt tgttttccca cactgaacca taaatatcgc cactagacgc
     2941 aacttgcgca ccacttccca gcgca
//
LOCUS       DAETQN010000269         2944 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.274, whole genome shotgun sequence.
ACCESSION   DAETQN010000269 DAETQN010000000
VERSION     DAETQN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.274"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            45..1120
                     /locus_tag="K0W68_004496"
                     /pseudo
     CDS             45..1120
                     /locus_tag="K0W68_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1136..1513
                     /locus_tag="K0W68_004497"
     CDS             1136..1513
                     /locus_tag="K0W68_004497"
                     /inference="COORDINATES: protein motif:HMM:NF012565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBI3075840.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKM"
     gene            1513..1884
                     /locus_tag="K0W68_004498"
     CDS             1513..1884
                     /locus_tag="K0W68_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBI3075841.1"
                     /translation="MQVTMQHALKLFWRPKAIELEDDGVMSYEKVEIIRRNDGSIRFN
                     NKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVGYINDFGGLSF
                     YEINCPTVNNSCNVSVAKRDK"
     gene            1886..>2944
                     /locus_tag="K0W68_004499"
     CDS             1886..>2944
                     /locus_tag="K0W68_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075842.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT          932 a          587 c          613 g          812 t
ORIGIN      
        1 gggcaactgg acgtcaaggt tgaagaagag gacgggccag tctatatatc aatgacacta
       61 accattacat gaattatcag aatgaaaaag agagttatat tgtcacgttt aaccagtatc
      121 ttgaatcatt aaggttaaat acatacgtaa gtttggctcg taatacttac tgggacgcca
      181 gcagtaatgt gaattattca ttatcactta gccgcgattt tgatatcggg ccattaaaaa
      241 acgtctccgc ttctctaaca tttagccgaa taaactggga agaagacaac caggatcaac
      301 tgtacctaaa tatttcgatt ccctggggaa ccagtagaac attgagctat ggtatgcaac
      361 gaaatcagga taacaagatt tcgcatactg cttcgtggta tgactcttcc gatcgaaata
      421 attcctggag cgtttctgct tcaggcgaca atgatgaatt caaagatatg aaagcgtcac
      481 tacgcgccag ttatcagcat aataccgaga acggtcgtct ctatctctcc gggacatcac
      541 agcgagacag ttattattct ctgaatgcca gttggaatgg ttcattcacg gcgactcgtc
      601 acggagccgc tttccatgac tatagtggta gtgctgactc gcgttttatg atcgacgcag
      661 atggtgcaga agatattccg ttgaacaata aacgcgcggt aactaatcgg tatggcatcg
      721 gagttattcc atcagtcagc agttacataa caacatcatt aagcgttgat acccgaaatc
      781 ttccagaaaa tgtggatatc gaaaactcgg ttatcaccac caccttaacc gaaggtgcta
      841 ttggctacgc caaacttgat accccaaggg ctatcaaatc atgggggtta ttcgcctggc
      901 agatggtagt cacccaccac tggggattag cgtaaaagat aaaaccagcc ataaagaatt
      961 aggactggtt gctgatggcg gctttgtata cctcaacggc attcaggatg acagcaaact
     1021 tactttacgc tggggtgata aatcctgttt tattcaacca cccaatagca gcaacttaac
     1081 caccggaacg gttattttac cgtgtattag ccaaaattaa ttcaggatga aactaatgcg
     1141 ctacttaaac acaaagaatc tcattgcagc aggagtatta ctcgcctgca tgagcagcat
     1201 tgcctggggt gccatcatcc cggaccgtac ccgtatcata atgaatgagt cagataaagg
     1261 ggaagcatta aaacttacta atcaaagcaa aaatctaccc tatctggcgc aaacatggat
     1321 agaagatacg aaaggcaata aatcacgtga ctttattgtc acggttcccc ctatggtacg
     1381 tttaaatccc agtgagcaaa ttcaaataag aatgataagt caggaaaaaa ttgctcaact
     1441 gcctaatgac agagaaacgc tcttctattt taacgtgcga gaaataccgc caaaaacaga
     1501 taaaaaaatg tgatgcaagt cactatgcag cacgcattga aactattctg gcgaccaaaa
     1561 gctattgaat tagaagatga tggggtaatg tcttacgaaa aagttgaaat catccgtaga
     1621 aatgatggtt cgatccgctt taacaataag atgccttatc acgttacgct gggttatatc
     1681 ggaacaaacg gaatcacaat gttgccacaa acgcaaagtt taatggtaac cccttatagc
     1741 catgcagata cgcagtttaa aaatgttccg tcagcttttc aggttggtta tatcaatgat
     1801 tttggtggat taagttttta tgaaataaac tgcccgacgg ttaataactc ttgtaatgtt
     1861 tccgtagcca aacgagataa ataatatgcg ctacttatta attgtaatta tatttattat
     1921 aggttttagt gcattacctg tatgggcaat ggactgctat gctgaacatg aagggggaaa
     1981 taccgtagtc attggttacg taccaagaat ttctatcccc agtaatggta aaaaaggtga
     2041 taagatctgg caaagcagtg agtattttat gaatgtcttc tgtaataatg cattacctgc
     2101 cccatcacct ggagaagaat acccatcagc ctggactaat ataatgatgt ttttaccagg
     2161 cggtcaggac ttttataatc agaactccta tatttttggc gtaacataca atggagttga
     2221 ttatgattcg actgccccta atgctatcgc agcaccagaa tgcattgata taaaaggagc
     2281 gggtaccttt aataatcact ataaaaaccc cgctgtctgt agtggaggtc ctgagcctca
     2341 attatcagta acctttccag ctcgcgtaca actctatatt aaattagcaa aaaatgccaa
     2401 tagagttaat aaaaacctgg tgttacctga tgaatatatc gctctagagt ttaaaggtat
     2461 gagtggcgca ggagctatag aagtagacaa aaatttgacc ttcaggattc gtggattaaa
     2521 taatattcat gtccttgact gctttgttaa tgtcgcactg gaaccagctg atggcgttgt
     2581 tgactttggt aaaataaatt cccgaacaat taaaaatacc agcgttagtg agacgtttag
     2641 tgtagtcatg acaaaagatc ctggtgcggc ctgtactgag caattcaata ttttagggag
     2701 ctttttcact acggatattt tgagtgatta tagccatctg gatatgggta atggactgct
     2761 attgaagata tttcataaag atggaacagc aacggaattt aaccgcttct cacaatttgc
     2821 ttctttttca tcgtctagtg caccttcggt caccgcacca ttcagggcag aacttagtgc
     2881 gaacccggca gagactgttg ttgagggacc gtttagtaaa gacgtaatcc tcaaaatcac
     2941 ctat
//
LOCUS       DAETQN010000270         2937 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.275, whole genome shotgun sequence.
ACCESSION   DAETQN010000270 DAETQN010000000
VERSION     DAETQN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.275"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            35..262
                     /locus_tag="K0W68_004500"
     CDS             35..262
                     /locus_tag="K0W68_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBI3075843.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(263..637)
                     /locus_tag="K0W68_004501"
     CDS             complement(263..637)
                     /locus_tag="K0W68_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HBI3075844.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="K0W68_004502"
                     /pseudo
     CDS             complement(720..1766)
                     /gene="mdtG"
                     /locus_tag="K0W68_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2097..2672)
                     /locus_tag="K0W68_004503"
     CDS             complement(2097..2672)
                     /locus_tag="K0W68_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3075845.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          762 a          695 c          687 g          793 t
ORIGIN      
        1 ttatgccggt atttttttac ggaagaggta tcaaatgcgt ttaattgtgg tgagcatcat
       61 ggtgaccttg ctgagtggct gtggcagcat cattagccgc actataccgg ggcaggggca
      121 cggcaaccaa tattatcccg gtgtgcaatg ggatgtgcgt gactccgctt ggcgttatgt
      181 cacaatcctt gatctgccat tctccctggt ttttgatact ttactgctgc cgatcgacac
      241 tcatcatggc ccgtatgagt gattaacgtt catcccactc atcagctgca gcgtgtcctt
      301 cctcggtatc cagcggtggc tcaagctgaa attccccctc gtcccattca tgtaatgtat
      361 tctcttcctg ccactcctgg cggatctcta tctcatcata gtcgccatca aaaacacttt
      421 gcgcggcttc accgctaagc ataggtaaac attcaccttc ttccccttcg tcggcaaaaa
      481 actcaacttg ccacatgatg tcgccgtcct gcaaaacgta tttttgggca ttgaactgtt
      541 gcacattcgc atcttcggcg tcgatgccgg ggttgtctgc aagaaattct tcgcgtgcag
      601 cgtcaatggc ttcttcaagc gttgcgtaca tggtcatcac gattctccct ttgagttgat
      661 gaggtttcag ggaaaaggat agctgattct ccgcttttgc aagtatgaaa ggcgaaaaat
      721 cagttcgata cctggggtat tcgacgacga cgtagactgt tccatgaata gactgcgttg
      781 aataacacta cgccagcggt gacgagaaat accgctctga aaccgtagtt cgctgaaatc
      841 gctgctccca tcaatggtcc ggtaacgttg ccaatatcac gaaacgattg gttatagctg
      901 aagatacgtc cggcgatttg gttgctcgag ttataaacta acagtgtctg tacggcgggg
      961 agtagtgcac catcggcggc accgagcaaa aaacgtaaaa tcccaagttg caatggcgtc
     1021 tgaacgtaag acattgggat caacagcagt acagaaaaga tcagcgccgt aatcaggatc
     1081 ttttcgggtc cgattcgatc gccaagtttg ccgagtcgtg gtgcacttag cagagccgcc
     1141 acgcctggca ccgaggcgat catgccactg ataaaggcga cgttactgac gttacccgcc
     1201 agttcgcgga catacagcgt cagaatgggg gcaattgagc ccgtcgccac ctggatgatt
     1261 aacgtagtga caaacaggct gagtaccagt ttcgggtttt taagtgatgt caccacttcc
     1321 cgcatgtgca gcatctcttt tttgctgatc ggctggaatt tttctcttat gcaaaacagg
     1381 gtgacgaaaa agcagagtat gagcacactg gcggtaataa agaataccgg acgtaagccg
     1441 tagctatcgg cgagcaggcc gccagccatt gggccgagca acgcaccact aacgccgcct
     1501 gtggagagcg tacccagcgc ccagccgctt ttattacgcg gtacttgtgt ggcgataaga
     1561 gcattagcgt tggggacaaa tccgccaagt aacccaagaa gcgcccgcag gatcaaaaac
     1621 tgccagatat tttgtgccag ccccatcaac accatcacga tgcccatgcc gagggcagag
     1681 cgtaatagca tgagttttcg gcctttacgg tcggcgagtc caccccaaaa cggtgaggcg
     1741 atggccgaaa ataaaaatgt aatgctcata tatgctatct taatgcacta cctgataagt
     1801 gagcctatac aggttattgc tgtatgttta tacagtatta ttggtagaag actgtgtaga
     1861 tttggtatag agatgaatag atactattaa ggtaacttat tgaaatcaat aaagattctt
     1921 gtgcagtctg aatagataac ttagcgttat gtctagtcgt ttccctctcc attcctaaaa
     1981 agtgctgtat actggcctat acaacgctat aaagacctct acatagcgcc ttgatagcta
     2041 tgtcacaggt acaaaaaagc cccctataaa ggaggctgtg tagtctatta agggtcttac
     2101 agggtcttgg tccagtgtct gctaacagtc cttacagaga ggttaagaac cttagctacc
     2161 tgagactgag atagtcccct ttccttgtac tctaagaccg ctctagttgt gtcactcgct
     2221 ataggtcttc caagtacctt accttgaact ctagcacgct ctagaccctg tttagtgcgc
     2281 tctctgatac ggtttctttc aaactctgca aaggtactaa atagctgtag aaccaactta
     2341 ccctctgagc ttgaaaggtc tttaacgggt aaatctaaac agatgagctt cacaccctta
     2401 tcattaagaa gggtcactgt attctgtagg tcgatgttat cccttcccag acggtcaagt
     2461 ttaagaacaa caagggtgtc accttcttct agcttatggt tgaccagatt cgcaaatccg
     2521 tccctatcca tagctgacac tgaaccagag acaacctcct ctataactcg tgacttagga
     2581 atatcatagc cagccttctt aatggcttga atctggttct ctgtggtgga agtctctact
     2641 gatacacggc agtaagcaaa ggttcgtgac atagatatcc ctctgggtag tcattaaacg
     2701 gttagtttgt cttgtctata gacattaaag caaatggtag atttgttgtc tatagctata
     2761 gtgactcttc tatgtagaca ttaaacggac gtttcttgtc gtgggtcaca tagatacttt
     2821 tagggcttct gtcaagtgat attcgtagat tttgcaaggg ggaggtgtaa ggctgaaaca
     2881 gggctactaa gaacacatac atctaaggct ggaaagtacc tgagcagata ttcttca
//
LOCUS       DAETQN010000271         2912 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.277, whole genome shotgun sequence.
ACCESSION   DAETQN010000271 DAETQN010000000
VERSION     DAETQN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.277"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..95
                     /locus_tag="K0W68_004504"
     CDS             <1..95
                     /locus_tag="K0W68_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3075846.1"
                     /translation="RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            83..732
                     /locus_tag="K0W68_004505"
                     /pseudo
     CDS             83..732
                     /locus_tag="K0W68_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
     gene            729..1208
                     /locus_tag="K0W68_004506"
     CDS             729..1208
                     /locus_tag="K0W68_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HBI3075847.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(1262..2212)
                     /gene="corA"
                     /locus_tag="K0W68_004507"
     CDS             complement(1262..2212)
                     /gene="corA"
                     /locus_tag="K0W68_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBI3075848.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2582..>2912
                     /locus_tag="K0W68_004508"
     CDS             2582..>2912
                     /locus_tag="K0W68_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075849.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          878 a          645 c          618 g          771 t
ORIGIN      
        1 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
       61 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc caagttccgg
      121 aacaaaaata tcacaacgtg aatgcactga tattttattt gcagaaatga aagaactagc
      181 aaaaatgttt tccttttttg gacaatataa aacacttatc gaagatctga ttgaacattt
      241 tagatatgga aatggcagca attttcatag ccaacaatta aatttatctt ttcatgaaaa
      301 aataaatatg gttataatag tccaatacga ataatcaaag aatgtatcga aaatggcatt
      361 aattcaactc cctcgacagg ataccaacct cttatcctgc aaagcattaa aaccaaatta
      421 ctttcatcaa gactaaataa atttaatgac ttcgaggaca gttttaatgg cttaggaatc
      481 agtgttcatg atatttcagc tcaaaaaata tctcttttaa gttttcaaaa atacgctata
      541 ggatggagtg caacaattca ttttgtagca caggaccact tcggtcttga tgtaactgat
      601 atcaaaaaca agacttacag caaatataga ttttttcgca tatggttctt tttacaacgg
      661 cataaagact ttgctttcaa accttttttc accaatttta acactatcga aagaatcgaa
      721 aattatctat gaaaagcaaa ctccagaaaa ttatattatg tttatttctt ctatgctgca
      781 tctataattt atggacattg cgtcctgtac agattttgta tacatactct gatgctggca
      841 attcagtctt tctggtcgtt gatcatctgc catggactga cagcgataaa ataaattggt
      901 atctgaagca tcagaatgaa attaaaaatc aacatcccct accagaaggt agttggcata
      961 cctggtatgt cattgatatc ggtaatggct tcactgacta taaaaaatat atcgaagggc
     1021 cgtatgaaga tctctactgt ttcccgacaa ttaagagcaa tgataattgc atcgtgaaaa
     1081 attacttaat ggttattaat gaataccctt atcgtaacac tcatattggt attaacgatt
     1141 ttactgagta tcaattaaca caagaaaata aaatagagcg agttttcaat ccgcatgact
     1201 ttaaataaga caatttttag aaataagccg gataaaaaaa tagctttatc cggcactcat
     1261 attacagcca gttcttgcgc ttaaagtaca gatacggtgc caggcccgcg aggatcataa
     1321 agataatcgc gcctgggtag ccgaaactcc acttcagttc tggcataaac tcaaagttca
     1381 tcccatagct ggaagcaacg agcgtcggcg gcaggaatac cacggatacc accgagaaga
     1441 ttttgatgat gcggttctgc tcgatgttga taaaacccat cgccgcctgc atcaggaagt
     1501 tcaccttctg gaacagggat tcgttatgcg gcagcaggga ttcgatatct cgcaggattt
     1561 cacgcgcctg ctccagttgc ccacccggta aacgcgcttt acgcaccagg aagttgagcg
     1621 cgcgctgggt atccatcaga cacaggcgaa ctttccagcc gatatcttcc agttccgcca
     1681 gagtggagag cgcctcgtcg tactcatcgc cctgatgccc ttccataatc acccggctca
     1741 actgctccag gtcgctatag atattttcaa tttcatctgc caactgttcg attttggttt
     1801 cgaacagatc cagcagcagc tcgtaggcgt taccgtctac catcgactgg ctacgggcac
     1861 gcatacgata cagacgaaaa gcgggcagtt cacgctcacg cagagtaaac agacgaccat
     1921 cacggatggt aaatgccaca gtggagttac cggcgtgatc ttccgcatct tcaaagaaga
     1981 agaaggagtg aatatgcagg ccgtcgtcgt cttcaaagaa acgtgccgat gcttcgatgt
     2041 cttccagttc cgggcgggtt gccaggctct ggccaagttc agattgtacg cgcagtcgct
     2101 cgtcgtcgtc cggttcgaca agatcaatcc atactgcatt tacaaggggt tgtgactctt
     2161 cgacttccag ccgggtcagt cggttatttt ccagttgaaa tgcgctcagc atgaccggga
     2221 ctcccaatgc ttaaaatatc ggacagttcg gtgggcacac agaaacaaat tgggtttcag
     2281 accattaaac agcctgactc agcgcgacgg gaaaaattga ggtcgctgac aaccgctaag
     2341 gctatcagca aaaagggata gccttaggag ttgatcctgg atgacaggat agtgagccag
     2401 tatctactgg gtgtgtccaa ggcgaatgtc ctcttagagt gatcgtgcgc gcatgttacg
     2461 ccagcaaaat tttgccgtca acacgcaaca gaacaccaca gaacaatagt tgaacttcgc
     2521 gctttttggt actcgtcaag gtcgctaaca ggataaaaaa atccaacatc caggacctat
     2581 aatggcgcat cggctactca ttggtaaagg aatgatcacc ttgaatctca aacggatttt
     2641 tctcgccctc accttacttc ccttatttgc tgttgccgct gatgattgcg cactctcaga
     2701 tccgatgctg accgtacagg cgtataccgt taatcctcaa acagagcggg tgaaaatgta
     2761 ctggcaaaaa gccaatggcg aagcgtgggg aactttacat gctctgctgg tggatatgaa
     2821 tagtcagggt caggtgcaga tggcgatgaa cggcggcatc tatgatgaaa gctatgcgcc
     2881 gctcggtttg tacatcgaaa acggtcagca ga
//
LOCUS       DAETQN010000272         2893 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.278, whole genome shotgun sequence.
ACCESSION   DAETQN010000272 DAETQN010000000
VERSION     DAETQN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.278"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..493
                     /locus_tag="K0W68_004509"
     CDS             <1..493
                     /locus_tag="K0W68_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBI3075850.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="K0W68_004510"
     CDS             493..1083
                     /locus_tag="K0W68_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBI3075851.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1070..2110
                     /locus_tag="K0W68_004511"
     CDS             1070..2110
                     /locus_tag="K0W68_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3075852.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTGFKQNGDGLLDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNG
                     GVQDVRYGYEMYYTPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQK
                     LINGTWYNVASI"
     gene            2113..2658
                     /locus_tag="K0W68_004512"
                     /pseudo
     CDS             2113..2658
                     /locus_tag="K0W68_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2682..>2893
                     /locus_tag="K0W68_004513"
     CDS             2682..>2893
                     /locus_tag="K0W68_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBI3075853.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEY"
BASE COUNT          753 a          632 c          824 g          684 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattacaccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctg
//
LOCUS       DAETQN010000273         2877 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.279, whole genome shotgun sequence.
ACCESSION   DAETQN010000273 DAETQN010000000
VERSION     DAETQN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.279"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..953)
                     /gene="curA"
                     /locus_tag="K0W68_004514"
     CDS             complement(<1..953)
                     /gene="curA"
                     /locus_tag="K0W68_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HBI3075854.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDG"
     gene            1134..1652
                     /gene="mddA"
                     /locus_tag="K0W68_004515"
     CDS             1134..1652
                     /gene="mddA"
                     /locus_tag="K0W68_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBI3075855.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1649..2098
                     /locus_tag="K0W68_004516"
     CDS             1649..2098
                     /locus_tag="K0W68_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI3075856.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2099..2332)
                     /locus_tag="K0W68_004517"
     CDS             complement(2099..2332)
                     /locus_tag="K0W68_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBI3075857.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="K0W68_004518"
     CDS             complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="K0W68_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HBI3075858.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2786..>2877
                     /gene="yncL"
                     /locus_tag="K0W68_004519"
     CDS             2786..>2877
                     /gene="yncL"
                     /locus_tag="K0W68_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBI3075859.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          691 a          738 c          692 g          756 t
ORIGIN      
        1 aaaccatcag taatatcttc gtggtagtgg attttatcct ctttcaccca ttgccccatc
       61 tccttctgaa actcatggat gcggtgacca taatcctgag cgataataaa cccttgcaag
      121 cgaatacgtt ttttcagcac cgttgccatc aacaggggta aacgatccgg accgggtggt
      181 agctctgtag cgttatagct gctcactaag ccacaaacgg gaatgcgcgc agatgtatta
      241 agtaatggta gcactgcatc aaatacctta ccgcccacgt tttcataata gatatcaata
      301 ccttttgggc acgctttcac cagttgttcg gcaaaatcat ccgcgtggtg atcaagacaa
      361 acatcgaagc ccagcacctc gatagcatgg cggcattttt ccgcgccacc ggcaaccccc
      421 accaccctgc aacctttaag tttgccgatt tgccccaccg ttgctcccac aggacctgtc
      481 gccgcagcta ccaccaacgt ttcgccctct ttaggctgac cgatatccag cagccccata
      541 taagcggtaa agcctggcat ccccagcaca cccagcgacc acgatggatt ttgcggatga
      601 tcgccaagtt tcaccagatc atcaccactg gatatgtcat agtcctgcca tccactgtag
      661 cccagcaccc agtcgctagg ctgataatca ggatgattcg actccacaac acggctcacc
      721 gtgccgccga ccatcacgcc gccaatatca acaggcggtg aataagatgg ctcatcgctc
      781 atacgtccac gcatatacgg gtccagggac aaataaactg tgcgcagtaa cacctgtcct
      841 tcacccggtg tggcgacatc atcttcttca agacggaaat tctccggaac cggtgcgcca
      901 tgtggacgcg aggccagaac ccaacgtcga ttacgctgct tttgttgccc cataatgctc
      961 tcctgtgctt ttggcatgaa tccatcaata gtagcaggct cacgggattg gttttgtttg
     1021 cagccgtcac atcccctctg caacacgcaa taatttcatc gaggaatccg ttgcttcggg
     1081 ctgttttcga tagtattgat aacatcatca tcattatttt atcgaggcca gacatgtcca
     1141 tccgttttgc ccgcaaagcc gattgtgctg ccattgcgga aatttataac cacgccgtgt
     1201 tgtatacggc ggctatctgg aatgaccaaa cggttgatgc agataaccgc attgcctggt
     1261 ttgaagcgcg gaccatagca ggttatccgg tgctggtaag tgaagaagat ggcgtagtga
     1321 cgggatatgc ctcgtttggc gactggcgta gtttcgatgg ttttcgccat accgtggaac
     1381 attcggttta tgtccatccc gatcatcagg gcaaaggtct ggggcgtaaa ttgttaagcc
     1441 gattgattga tgaagcgcgg gattgcggga agcatgtcat ggtcgccggg atcgaatcgc
     1501 aaaatcaggc ctcgctgcat ctgcaccatt cgctgggatt tgtcgtcacc gcacaaatgc
     1561 cgcaggtggg cactaaattt gggcgctggc tggatctgac atttatgcag ttgcaactcg
     1621 acgaacgcac tgaaccggac gcgattggat gaatcagtcg ctcacccttg cttttttgat
     1681 tgccgccgga attggtctgg tagtccagaa cacgctgatg gtgcgcatca cccagacctc
     1741 ctctaccatt ctcatcgcca tgttgctgaa ctcactggtg gggattgtgc tctttgtttc
     1801 tattttatgg tttaaacaag gcatggcggg gtttggcgaa ctggtgtcca gcgtgcgctg
     1861 gtggacactt atacccggct tgctgggatc gttttttgtc ttcgccagta tcagcggata
     1921 tcagaatgtg ggagcggcga caactattgc agtactggtc gccagccagc taattggcgg
     1981 tctgatgctg gatatcttcc gcagtcatgg cgtaccacta cgggcgttgt tcggcccaat
     2041 ttgtggcgca attttacttg tggttggcgc ctggttggta gccagacgct cattttaatc
     2101 agaggatggt gccgcctttg gttaactgtt cgtggcgcgc ttccatatct tctttatgct
     2161 tgcgaccgtg atgagcaata gcggtgcgca aacgctgctg ctgggtgtag cgatcttccc
     2221 gacttaactc cgcgtcatcg ctcagggcga ttaataattc gttcatatgg gcaatgacgc
     2281 tctcttcaat cgcggcatcc acgcgcgcga tgacttcatc cagatgcgac atactttctc
     2341 cttaaccact tgtgaaattt atcgattcat ccaaatgata taagtctgcg tggtgccgtc
     2401 aagccagtga gtaagattta aaacagtgaa aacaacagcg ccaccgggaa actcatcggc
     2461 catgttgccc ccaccagaaa ggcgcttaag aaacggatgc gtttcttatc gtgagaaaga
     2521 aaccaggtga taagaaatgc gattgctgcc ataaccgcat aaaaaacaag catatgttga
     2581 tagagagaca tgtacgggcg tccagccaga tgaataaaac gcgcgcaaat atgcgtcatt
     2641 ttttgacgtg catcaagttt tttcaccagt ttgagaaaaa atcatcaaac agcaacttgc
     2701 ccttatccat cttcagaatg agtattattg cggattttct taacccgtac tatacacata
     2761 ggggcaatga taaaaggtgg caaaaatgaa tgtttccagt agaactgtag tactgataaa
     2821 tgtctttgct gctgttggtt tgtttactct tatctctatg agatttggct ggtttat
//
LOCUS       DAETQN010000274         2838 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.280, whole genome shotgun sequence.
ACCESSION   DAETQN010000274 DAETQN010000000
VERSION     DAETQN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.280"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1118..1453)
                     /locus_tag="K0W68_004520"
     CDS             complement(1118..1453)
                     /locus_tag="K0W68_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HBI3075860.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            1582..1929
                     /locus_tag="K0W68_004521"
     CDS             1582..1929
                     /locus_tag="K0W68_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075861.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1949..2539)
                     /locus_tag="K0W68_004522"
     CDS             complement(1949..2539)
                     /locus_tag="K0W68_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075862.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLPACHDAGMEVRVQINESAAP"
     gene            complement(2536..2796)
                     /locus_tag="K0W68_004523"
     CDS             complement(2536..2796)
                     /locus_tag="K0W68_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075863.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT          795 a          671 c          588 g          784 t
ORIGIN      
        1 tcacataaac caccgattga gtgacagtta ctgaaacaac tatttcgaca caaaatctcg
       61 tctattcaac atcatgcaaa tttgtcagtg actgaattta gtgacagaac cctctggaat
      121 tctgccgtgt aaccacttat atgcagacct gccccgttgc tctgggtttt caggctgcga
      181 tgagtgatga gcgatgattt ccatacagat gatatatcga ctggaattga ttgcctgcaa
      241 ttagatttag ttttattgtg tggatcgttt tagttagtgg ggatgaattt ccgataaatt
      301 aaccaatgga aaggagatga tatgaataaa aagataactg gattattaat aattagtctg
      361 gcgtcaattt tcttaattga ttgcacccag tgccattaac atctatctac acaaaaatag
      421 aggaaaacaa ttttgatata tcaaataatt acaagcagct ccattctttt tattgtttgg
      481 gggattacat ctataataat tagaacaaag aattattttt ctaagaagaa aacagacctt
      541 aatataagct tttttatagc cttccctatt tctataacaa tccttttatt ttgccgaggt
      601 atattataaa tataacataa tacatctata tggggtgtac atatccccac ataaaaacac
      661 atactctcat taacacggga agaaactatg ttttccaatt ctcactacat gtacataatc
      721 gttcttgtac tatggtttag tattctcaag tacttacaga gaaagaatag catattacat
      781 cctttatttt cactaatcct gacaccacta accattttaa tcattagact tatgatagat
      841 ttaacataca accaataaat tataaacaaa ataaaaatag ggaacattat atgctttata
      901 aaattttcac attaaacaca aagataaaaa caactatcat tacaatatgt gcattatcat
      961 ataatcttac agcgcaagct tccccgtcat acagacctcg tgatcgagtt aataagacta
     1021 tgatagaact acaaactaac ggagtagaac ggcaactttt agataattct tgtggcataa
     1081 gttctggggt atttaaataa aatataagac aggctgtcta tcttacagac agccttttta
     1141 taataacaga aagaatatat atcatactta acggaagaga taatatgaaa acagtacccg
     1201 tataaacagc aagcaatccc atttttccag gtgtatcagc aaaaaaaacg ctgttccaga
     1261 aatcaggccg ggtaaatttc agagctgtat cttcaatata ccattttgca accggataca
     1321 aaaccattcc gcagagagaa atacaccaga acagtaatct gtatttaaaa tcataatccc
     1381 atgacatata cagcatatat cctcccgtca cccatcccca ccacatatta ttaaaataat
     1441 attttctgtt catctgccag ttttcctttc ttttgcactt ttgtcagtgt actgatgcat
     1501 gacaacataa ccacagcatg ataataataa tcaataacaa taagctgtgt cacgtttaca
     1561 tcatggaacg gaggtgctcc tgtgactttt gagcagaaaa aagcccgggc cattgccctg
     1621 atggacagca aaaagatgtg gcggagcaat tatgcccctc ctcttctgcg tattctctgg
     1681 cggctgggta tccggcttcc gccattaccc tttatgccgt tctggcaggt cacggtgctg
     1741 acggggggct tatggggcat ttcctgggga tgtgcaatgt ggtttatcta ctgggggccg
     1801 tcaggaatgg tggcaggtga ggccattatt ataagtatca cggggggttt tttgtccgga
     1861 ctgctcatgg cctctttcca ctggtggcgc aggaaagtta accgactgcc accatgggat
     1921 gatgtgtaaa ccgtgcacag gcgtgttctt acggagcggc actttcattt atctgtacgc
     1981 gaacctccat tcctgcatcg tgacaggccg gcagccactg cgccacttcc agcggatcgc
     2041 cctcccggcg taccacccag ccttctttat tccataactg cagacaggtg ctgccgtcga
     2101 gacgcaccac aaaatcccca cggcaggcct gatacgttat gccgttcagc tccaccgcca
     2161 gatcagtggc agaagtgccg cctgcgggca gacctgctgc cgggaccagc ggcagtacga
     2221 cgacttctgc cgcacgctgt tctgcccgga tgcggtcctg ttctgccagc agcagaggct
     2281 gtgccacggc ccggccggcg tccgtcagct ccactgccag ctgcaggttc ggagcgcgca
     2341 gggtacgcag ccagccggca tcctccagac gacggcagga ggcgcgcagg tttggcccgt
     2401 agaccggggc ttccccgctt tgctccagca cccgttctat gtcccgcgtc agcacgggtc
     2461 ctggccgttt ttcatcaagg gcagccagca caatcagcac tctccgctgc agtggcgacg
     2521 gtcggcggct ctgagtcatt tccagtcatc ctccggccag atgagctttc tgaggcgctg
     2581 atggtcggcg tcaatgatct cctgtctctt tatggcattc attgaggtat agccatttat
     2641 ggccagttca tgccagaaga tcagcacggc tccggctttg gctgactcgc agagataagt
     2701 tacgccgtgg ctgtccagat tgcattcccg gatgcgtttt tccgcaaacc gggtcagttg
     2761 ttcatagctg agtgaaaacg tcatttcctg atccatcctg cacgttccat ctgataatgt
     2821 tctgacaggt aaaaatca
//
LOCUS       DAETQN010000275         2787 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.281, whole genome shotgun sequence.
ACCESSION   DAETQN010000275 DAETQN010000000
VERSION     DAETQN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.281"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..411
                     /locus_tag="K0W68_004524"
                     /pseudo
     CDS             1..411
                     /locus_tag="K0W68_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            996..1712
                     /locus_tag="K0W68_004525"
     CDS             996..1712
                     /locus_tag="K0W68_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075864.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYILARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2264..2668
                     /locus_tag="K0W68_004526"
     CDS             2264..2668
                     /locus_tag="K0W68_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075865.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
BASE COUNT          808 a          533 c          611 g          835 t
ORIGIN      
        1 gaagacccag atatgaacag gcacattcag agttacttta aagagcttac tgaacgtctg
       61 aggtgttaga gccatgactg ccgcagcatt gctggcggaa atgcctgaat cgagcagcct
      121 ctcgagacgt gaaatcagcg cacttgtcgg tgttgctcag gtaaaccggg actcgggaac
      181 tctgcgcggt cgacgaacca ttttcggtga ttgtgctggg gaagaacagc tctgtacatg
      241 gcggcgcttg aggcaccccg gtttaatctg gtgataaagg cgttttacat gcgcctactt
      301 gcagccggaa acgccaaaaa agtggcactg gttgcctgta tgcgtaagtt gctgaccatt
      361 atgaacgcaa tgctcagaaa aaacgaagaa tggaatgaat cgtatttatg aaaactggat
      421 tgtgggccat aaatattcca aaaagatagc ccgctccgtt tattaccgga acgggcgaga
      481 gcatgacatt gtctgtaatt ataatgagac tgctgattct acttattttt aatttcaaga
      541 aaagcgtttt ctacaaagaa ttatgagatt taatgttttg tttagacaat attatcattc
      601 tgttcaataa tgaaagtctt catcaacaac aagtaaaaac aacgtccctc tcatcgaacc
      661 ggacttgcag atttcccgca tccggctctc ttgcaacaca tcagaccttc acgttcgggc
      721 ggttatgggg aaggtccagc aagctgtatg agccctaact gtttgaatac cagtagatta
      781 ccagaaactt ctatcgattg aagacattac caactagcgt tactaaatgt tgaccaagtt
      841 ttgttcactt gtctggtttt gatagtagca ttctgcagag tattttgata tgataaatat
      901 gcattaacaa tgatattatt gttaatttcc tagcatgaaa attaaaagat caacttttta
      961 tctcgaatat tttttacatt atcagttggt ttcttatgaa tgacaattca ttgcttagaa
     1021 attcttcatt attcatcgcc tatatgggat gtgtagggtg ggtcagtgct tattcttatg
     1081 gctggggaac ctctttttac tatggatttc catggtgggt tgttggtgca ggtttggatg
     1141 atgttgccag aagtttactc tatgctatca tagtaatggg tatacttttt accggttggg
     1201 ggattggtat tttatttttt cttcttataa agaaaagaag taaaatacaa gacttgagtt
     1261 tttttagact attttttgct attaccctac tgtttttccc tgtaatattc gaacttttaa
     1321 ttctcaaaca atatttcata cttccattat cactttcatg cattattagt tcattggtta
     1381 tttcaataat aattcgtata tatggacgga tcttttctgt atcatgtttt tcagatatcc
     1441 cttttgttag agaacaccgc attaagttaa ttatggctgg ttttttggtt tatttttggc
     1501 ttttttcctt tcttgttggc tggtataaac ctcaattaaa aaaagaatat cagatgcttt
     1561 gttataataa tagttggtat tatattcttg ctcgttatga ttcacggctc gttttgtcta
     1621 gttcatttaa agatgacagt aacagattct taatatttaa tacagaacag tcgggatttt
     1681 atgaaatcaa tgatgtttat gtcagaaagt aataatcctc tcccacaaag tgaattaaat
     1741 gaatatataa tgcgttcgaa gtatatgtat tgagctgtca tactgagaaa caaaccttag
     1801 ggcagcacaa ttaaagccag aaacaactat gcaagaaagt tactaaataa gtgctcggaa
     1861 cagtaaaaca ctcagggggg actcagtccg gattatgtaa tcagataaat tgccaaacat
     1921 cacaaatcac cagccggact gagtgatgcc gagcattgca ccaatacccc gtgatgaacg
     1981 atgcctgatg cagaaagctc cctataaaac aaagaacaaa aactacgacc ggcgattcac
     2041 cgtcatactg atgctgcatc gtggtgacac tgtctgtcac tttcatgttg actgaacaac
     2101 tctgctgcga agaaggtgta ccgccggcgg gatttgattg tccggcgtct agcttccact
     2161 tccctgatgg aactgaaagt tgttatggcc ggtcagcttg ccctgtccgc aggacatgag
     2221 gtgctgtgac ggacagcacc agtaacggaa agtgacaatg aaaatgaata ctgcgctgat
     2281 agttgcacta atgtgtatgt ggtatgcggt acccgctgct gcaaaagaga ctttactggc
     2341 tatgccacgt aattcaacag aacattgcta tgcggagatt aatgtgcacg gaccatatgg
     2401 ggtgtatttc cgagtggtcc cgcatccccc cgggggaaag agctgggtcg aatgtaacag
     2461 tgactactat tactctgata aaccaccggg agtacagata ctcggaaccc gtgcagggtg
     2521 tcgggtatac ggaatctgcg ggaccacctc gacactgcat gtggctggaa gaggggttgt
     2581 gtgtattaaa aatatatgca gtccacgtgg gatgataatt cacaggatac ggaaacgtcc
     2641 tgtagtggct gtaagcgatg agatgtgatc ttacccagca atagtggaca cgcggctaag
     2701 tgagtaaact ctcagtcaga ggtgactcac atgacaaaaa cagtatcaac cagcaaaaaa
     2761 acacgtaaac agcattcgcc tgaattt
//
LOCUS       DAETQN010000276         2739 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.282, whole genome shotgun sequence.
ACCESSION   DAETQN010000276 DAETQN010000000
VERSION     DAETQN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.282"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="K0W68_004527"
     CDS             complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="K0W68_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HBI3075866.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
     gene            1322..2374
                     /locus_tag="K0W68_004528"
     CDS             1322..2374
                     /locus_tag="K0W68_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HBI3075867.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
BASE COUNT          849 a          592 c          616 g          682 t
ORIGIN      
        1 gccataaaga aaggaaatgc gctcaacgtt ccgattaacg cacctgtgat ataaacccta
       61 cggcgaccaa aacgatcggc aagccaggca aaacagggaa ttgtcaggca gcttaatcca
      121 cctaccagca aaccaatatt aaggaaaagt tcgcgcggta gccccatatt ctgggttgaa
      181 taattaagtg caaaggcagt aacgatgtac atcgtcagca attcgcacag tcgtagcgca
      241 ataatcttca ggaaagcacc gggatgtcgt aacagcgctt cgataaccgg gatgcgtttt
      301 ttcgcggcag cttgataatg ttgctgttgt tcaaattccg cggactcctc catgccattg
      361 cgcacccaca atgctcccag taccagtacg atgctaaaca ggaaaggaat gcgccagccc
      421 cagcttaaaa actgttcgtc agtcgtcatc atactgatca atgaaaccag tccggttgaa
      481 agcagtaaac ctacaccgta gccaacttgt acaccgctac tgtaaaaggc ttttttattt
      541 ttcggtgcac tttcaacgga aagcaacgcc gcgcctcccc attcgccgcc gacggcaaat
      601 ccctgaatga cacgcagtgt caccagtaaa ataggtgccc accacccaat ggtcgagaat
      661 gaaggaagaa taccaatcaa ggctgtcgcg atgcccatca tccagacggt cagcattaac
      721 atgcgcttac gtcccagtcg gtcgccaaag tgaccgaaaa tgacaccgcc gagcggacgg
      781 aaaaggaatc cgacgccaaa ggtagcaaat gcggcgagcg ttcccatcgc cgggcttact
      841 tgcgggaaaa actcgcgatt aaacaccagt gcggcggtga tgccatagag taaaaaatca
      901 taccagtcga cgacggcacc agcgaagctg cctaacgcag ctcgtcgggc gcgacttaac
      961 gaaagcgtcc cttcatcggg acgagtggag atgagcgtgg agtccataat gatcctgttt
     1021 gtactgtctt tttataatta gtatggaaaa ggtaactcac attgccgtcg atctataaac
     1081 gaaccttatg agactacctc gacagagcgc atctgcaaat gattttatgg ctgattagcg
     1141 gcaacatgaa aaattcgatg gaagatgaaa gcgcaggagt tgctggtgtt gtttaaggat
     1201 taagcagagg acccacaggt tagagatatg aaacataaaa gtccgcatat gcacaaaatg
     1261 gcaccctgat gagtctgata gactgcattg cattataacc agtagggagg cgaggttagg
     1321 tatgttttta gcatcattgt tgagacgtat tgcatttagt tactacgatt ataaagctta
     1381 taatttcaat attgaaaaaa cagactttgt tgtcatccat attcccgatc agattggcga
     1441 tgctatggcc atctttcctg ttattcgggc gcttgaattg cataaaatta agcatctttt
     1501 aattgtaacg tcgacaatta atttagaagt ctttaatgcg cttaaacttg aacagactaa
     1561 attaacatta gtcacaatga ctatgcagga tcacgcaaca ttaaaagaaa taaaagattt
     1621 agcaaagaac ataacacagc aatacggtac gccggatctt tgtattgagg ggatgcgtaa
     1681 aaagaacctg aaaacgatgt tatttatcag tcagttgaaa gcaaaaacga attttcaggt
     1741 tgttggtata accatgaatt gctactcccc tttgtgcaag aacgcgtccc gtatggatca
     1801 gaaactccgg gctcccgtac ctatgacatg ggcatttatg atgcgtgagg cgggttttcc
     1861 agcagtcagg ccaatatatg aattgccact aagtgaggat gtactcgatg aggtgcgcga
     1921 ggaaatgcga tcgttaggat cttacattgc gttcaattta gaaggtagct cgcaggaacg
     1981 tacattttca ttatcgattg cagaaaatct aatagcaaaa attcaaagtg aaacagatat
     2041 accaatagtg atcgttcatg gacccaaagg tgaagataaa gccagggcat tagtggattg
     2101 ttataataat gtctaccgtt tatccttacc accctcgatt aaacgttcag cagcaatcat
     2161 aaaagatgct tatatcgcaa taactcctga cacctcaata ttacatatgg caagtgccta
     2221 taatacccct gttgttgcaa tttatgctga ttacaaaacg cgatggcccg caatggccga
     2281 tgtctcggag tcagtcgtcg ttgggcaaaa aattgacaat ataagtctgg atgaattcgc
     2341 aaaggcatta aaaagtgttt tggcgagaat atgagacagt acttatgcat agtaatgccg
     2401 atctgttatg aacggtggtg tcatcaatac tctgaggctg gtcaatcatc acagaaaacc
     2461 agcctttgaa tcaaagcagt actcaccgga tacgatcata gtcacatcga acgcaattct
     2521 ggcggtgccg catttttatt tattcatttt ctgtgagaga agggcaagct ctatagaggg
     2581 aatgtaaaaa agagagctcc gaatggagca ctctttaaga aattataacg gaaaatcaga
     2641 ggtttttata acatgtcgca taagcaaacc caagcttttc aaatacgctt tgatcacctg
     2701 ttgccaggga aactacgtat tgttcgcttg agtttgcgg
//
LOCUS       DAETQN010000277         2730 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.283, whole genome shotgun sequence.
ACCESSION   DAETQN010000277 DAETQN010000000
VERSION     DAETQN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.283"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..328
                     /locus_tag="K0W68_004529"
     CDS             <1..328
                     /locus_tag="K0W68_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075868.1"
                     /translation="NIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVRVDEKERQVRC
                     KICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIERNAKARIRRA
                     TKSRTE"
     gene            421..639
                     /locus_tag="K0W68_004530"
     CDS             421..639
                     /locus_tag="K0W68_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBI3075869.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            641..1006
                     /locus_tag="K0W68_004531"
     CDS             641..1006
                     /locus_tag="K0W68_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBI3075870.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            1003..1668
                     /locus_tag="K0W68_004532"
     CDS             1003..1668
                     /locus_tag="K0W68_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075871.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1668..2033
                     /locus_tag="K0W68_004533"
     CDS             1668..2033
                     /locus_tag="K0W68_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBI3075872.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2488..2643
                     /locus_tag="K0W68_004534"
     CDS             2488..2643
                     /locus_tag="K0W68_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBI3075873.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          755 a          576 c          730 g          669 t
ORIGIN      
        1 caatatcgtt ttatttccgg taaaagagga tgacccacgt aatcaggtta attttcttta
       61 tgaaccatcg gaaagaccat attgccatca cgcctctgtc cgggttgacg aaaaagagcg
      121 tcaggtccgc tgtaaaatct gcggtgcggt tgtggagccg tttgactgga tgctctctgt
      181 ggcaaaaaga gaaaccagac tggcagatga tgtaaggcta ttgcgccagg aggaacagga
      241 aaggcggaga aatatagaaa agctgataca gattgagcgt aacgcgaaag cgcggatacg
      301 cagggcgaca aaatccagaa ctgaataatt aaatttagca ctgttaaaaa tttaatcctt
      361 aaccggaggg atttctgcac cctcaaatca tcaggagacc gcccgaaagg gcggtaatga
      421 atggttacat tatttagaaa aaaatatccg cgaaagagta gaacaacaga attcctgttt
      481 ctcattctgt ttatcgtgtt gatgacaccg atatccccgc taatttttgt ctggataatc
      541 ggaaaaataa ttgagccagt tattgaattg tataacgacg tggtatgggc gtcattcaac
      601 acactgcaca ataaaattaa tccgtataag gaaagctgat atggcactga cgaaaaaaca
      661 acgtgcagaa ctgcgcatga agttcggtgg tcgctgcgct tattgcggct gcgaacttgg
      721 cgaaaagtgg catgcagacc atgtaaaacc ggtcattcgt tttgatggaa atatgcttca
      781 ccaggaacgt gacgatatat ccaacatggt accagcatgc cacccatgca atctgcacaa
      841 gcattgcagt agcctggaag attatcgacg aattatcagt gatggtcgtc gtgaattcct
      901 tgcgtccggg aaaggcaaag cgctggttcg tatgggattg gttgaaatga aatctgaccc
      961 ggttgtgttc tggtttgaaa aatatcaaga aggggctacg gcatgaccac tattaccaaa
     1021 gagcgactgc aatggctggc taacatttct ggccgcgatg atattgacga tatagacggc
     1081 ggtgaaattc gtgaactggc gcttatcgct ctggcatcac tggaagcaga gcctgtagcg
     1141 gaatgcattg ttgaagatgg gggcatgtgt attgacgggt tcggtgagta tgtgggtcac
     1201 tcgctgcctg atggaatgca tgagctttat gctgccccgc gaatgcgaca acctgatgga
     1261 tggaaagccg tagctgtagc gtggaaggtg acgtttactc aagttgacca ggaatctaat
     1321 acgttcactg ctatatattt tgacaaagcg gaagttgagt gttgggtacg actgcataaa
     1381 gcatgtgatt ttcgggcaga tataacaccg ctttacgcag cgccggcagt gccggttgca
     1441 gtaaacgacg acatggctta cgcattccat cacgcactgt cagattcatc gcttggctct
     1501 gatgaaatcg aagaaattaa aaccggtttg cgtgctgcct ttgccaacgt cactatccaa
     1561 ccagagccga tagtgccgga tgaaatcggg ccaaacgata gtaatacgtt tgattatgtt
     1621 gatggctgga acgcctgccg cgctgccatg cttaagggag ataaataatg attaatcgaa
     1681 ccaaactgga gcacattctc gaatatgcca ggcagcagaa atgtattggg caactttgta
     1741 aaattccacc aggagatatg gttgaaatcg tggaaatggc catgcgtaag gctggcaact
     1801 ctccggtagc tccggctggc tggataagct gtagtgatgc agttcctgct gaatattgcg
     1861 atgtgattct tctcgatgat ctcgggaatg tattccccgg ttcctgggat aaggtttttt
     1921 gccccactcg tggcgggaat aagatggctt ttgtggacaa agacggcgtt gaagtagaga
     1981 gctcaactca ctggatgccg ctaccggaac caccgcagga ggtgaatcaa tgacctggcc
     2041 tgaggcattc accacggtag gaattgcgat ggcggtggcg ctggtggtgt attcgatttg
     2101 ccgttggggt taacaaacaa aaaccccgga ttgatggtcc ggggtttttg aaggaaacaa
     2161 aacagaaaca gcaattgccg ttacctgttg ttaccatggc aagtaaacgt atctcaggcg
     2221 agcgcattgc gccgttctga cgcagataaa ctagcctgga tagatggtgc tggcaataaa
     2281 aaatagcgtt ttcttatcgg tgtcggtaag attgctgcgg gtgcttgagg ctgtctgcct
     2341 cgggtatgtc actgtaaggc agacagagaa aagccccagt taacattacg cgtcctgcaa
     2401 gacgcttaac attaatctga ggcccaatct atgtctcaca aatgtaggtt agcctcttac
     2461 gtgccgaaag gcaaggagaa gcaggctatg aagcagcaaa aggcgatgtt aatcgccctg
     2521 atcgtcatct gtttaaccgt cattgtgacg gcactggtaa cgaggaaaga cctctgcgag
     2581 gtacgaatcc gaatcggcca gacggaggtc gctgttttcg tagactacga atctagagag
     2641 taagagtgac caggcgggag agtaatctcc cgccaccttt gatgtgtcag gcatcctcaa
     2701 cgcacccgca cttaacccgt ttcggcggtt
//
LOCUS       DAETQN010000278         2727 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.284, whole genome shotgun sequence.
ACCESSION   DAETQN010000278 DAETQN010000000
VERSION     DAETQN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.284"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(423..680)
                     /locus_tag="K0W68_004535"
     CDS             complement(423..680)
                     /locus_tag="K0W68_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075874.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="K0W68_004536"
     CDS             complement(677..1174)
                     /locus_tag="K0W68_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HBI3075875.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="K0W68_004537"
     CDS             complement(1376..1834)
                     /locus_tag="K0W68_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBI3075876.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="K0W68_004538"
     CDS             complement(1831..2328)
                     /locus_tag="K0W68_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBI3075877.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="K0W68_004539"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="K0W68_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HBI3075878.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          648 a          708 c          620 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatatct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataacc
//
LOCUS       DAETQN010000279         2722 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.285, whole genome shotgun sequence.
ACCESSION   DAETQN010000279 DAETQN010000000
VERSION     DAETQN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.285"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            31..2547
                     /locus_tag="K0W68_004540"
     CDS             31..2547
                     /locus_tag="K0W68_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI3075879.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            2558..>2722
                     /locus_tag="K0W68_004541"
     CDS             2558..>2722
                     /locus_tag="K0W68_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3075880.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          779 a          657 c          630 g          656 t
ORIGIN      
        1 acaaatataa aaaacacagg tcatcaggga atgccacaac gacaccacca gggacataaa
       61 cgcacaccga aacagttggc gctcatcatc aaacgttgtt tgccgatggt gctcactggc
      121 agcggcatgc tttgcactac cgctaacgcc gaagagtatt atttcgaccc cattatgctg
      181 gaaactacaa aaagtggtat gcaaacaacc gatctgtcac gtttttcaaa aaaatacgca
      241 caactaccag gaacttatca ggttgatatc tggctgaata aaaagaaggt ttcacagaaa
      301 aaaattacat ttaccgccaa tgcagagcaa cttctgcagc cacagtttac ggtagaacaa
      361 ctacgtgagc tgggtattaa ggtggatgaa atcccggcgc tggctgaaaa agatgacgat
      421 agcgtgatca actcgcttga acaaatcatt cccggtacag cagctgaatt tgatttcaat
      481 catcagcgac ttaatttgag cattccccaa attgcactgt accgtgatgc aagaggttac
      541 gtctcccctt ctcgttggga cgatggtata ccaacgctgt ttaccaacta ctcgtttaca
      601 ggttctgata accgttaccg ccagggcaat cgtagccaac gacagtacct gaatatgcaa
      661 aatggtgcta attttggccc ctggcgatta cgcaactatt ccacatggac acgcaacgat
      721 cagacatcaa gctggaatac catcagtagt tatttacaac gtgatatcaa ggcattgaag
      781 tctcagttgc ttctgggaga aagcgccacc agcggcagta ttttttccag ctacaccttt
      841 actggcgttc aactcgcttc cgacgataat atgttgccaa acagccagcg cggatttgcc
      901 ccaacggtac gcggtatcgc aaacagtagt gcaatcgtga ctatcaggca aaatggttat
      961 gtgatctatc aaagcaacgt gccagcgggt gcctttgaaa ttaacgatct ctacccctct
     1021 tccaacagcg gcgatttaga agtcacgatt gaagaaagtg acggtacgca acgtcgcttt
     1081 atccagcctt attcttcatt acccatgatg cagcgacctg ggcatctaaa atatagcgcg
     1141 accgctggac gctatcgcgc tgatgcaaac agtgatagca aagaacccga atttgctgaa
     1201 gccacggcaa tatatggttt gaataatact tttacgctgt atggtggcct gctcggttct
     1261 gaagattatt atgcgctggg gatcggtatc ggcggcacac ttggcgcact gggcgcgttg
     1321 tcgatggata tcaacagagc tgacacccaa ttcgataacc agcactcttt tcatggctat
     1381 caatggcgta cgcagtacat caaagatatc ccggaaacca acaccaatat cgctgtcagc
     1441 tactatcgct ataccaacga tggctatttt agttttgatg aagccaatac ccgtaattgg
     1501 aactataaca gtcgccaaaa aagtgaaatt caattcaaca tcagccagac aatatttgat
     1561 ggggtaagtc tgtatgcctc cggttcgcag caagactatt ggggcaataa cgataaaaac
     1621 aggaatatct cagttggggt ttccggtcag caatggggaa ttggttacag cctgaattat
     1681 caatacagtc gctacacgga tcaaaataat gaccgcgcac tctctttgaa tctcagtatt
     1741 ccgttagaac gctggttacc gcgtagccgg gtttcctatc agatgaccag ccagaaagat
     1801 cgcccaaccc aacatgaaat gcgtcttaat ggctcactgc tggatgatgg tcgcctgagc
     1861 tatagcctgg aacaaagtct ggatgacgat aacaaccata acagtagcct gaacgccagt
     1921 taccgttcac cttatggcac cttcagtgcc ggatacagct acggtaatga cagcagccaa
     1981 tacaattacg gcgttaccgg cggcgtggtt atccatactc atggcgtgac gctctcgcaa
     2041 tatctgggca acgcttttgc gcttatcgat gctaatgggg cttctggcgt gaggatacaa
     2101 aactatccgg ggattgctac tgatcccttt ggctatgcag tggttcctta tctcacgact
     2161 taccaggaaa accgtctctc ggtagatact acgcagctgc ccgataacgt cgatcttgag
     2221 caaacaacac agtttgtggt gcccaacaga ggtgcaatgg tagcggcgcg tttcaacgcc
     2281 aatatcggtt atcgcgtact tgttatagtc agcgatcgca acggtaaacc gttgcccttt
     2341 ggcgctcttg ccagcaacga tgagacgggg caacaaagta tcgtcgatga gggcggcata
     2401 ctatatctct ctgggatatc gagtaaatca caaagctgga ctgtacgctg gggaaatcag
     2461 gcagatcaac aatgtcagtt tgcttttagt acaccggatt cagaaccaac aacctctgta
     2521 ttacaaggca cggcgcagtg ccattaagga taaaaaaatg aaaagagcgc ctcttataac
     2581 aggacttttg ttgatatcca catcctgcgc ttatgcctcc tcaggagggt gtggagctga
     2641 cagcactagc ggtgcgacaa attacagcag tgtggttgat gatgttacgg tgaaccagac
     2701 agataacgtg acaggacggg ag
//
LOCUS       DAETQN010000280         2702 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.286, whole genome shotgun sequence.
ACCESSION   DAETQN010000280 DAETQN010000000
VERSION     DAETQN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.286"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..246
                     /locus_tag="K0W68_004542"
     CDS             <1..246
                     /locus_tag="K0W68_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBI3075881.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
     gene            complement(286..1494)
                     /locus_tag="K0W68_004543"
     CDS             complement(286..1494)
                     /locus_tag="K0W68_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3075882.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            1502..2003
                     /gene="tnpA"
                     /locus_tag="K0W68_004544"
                     /pseudo
     CDS             1502..2003
                     /gene="tnpA"
                     /locus_tag="K0W68_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(2021..2689)
                     /locus_tag="K0W68_004545"
                     /pseudo
     CDS             complement(2021..2689)
                     /locus_tag="K0W68_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          626 a          665 c          663 g          748 t
ORIGIN      
        1 gctgccctgc ccgtaagttg gatccagatg ctggcaggtc tggcgctgtt gagtaccatt
       61 ggcggcagtt tgtatcaggc gctgcataat gagcgtgagc gtgagcgcga cgcggcggtg
      121 gtggcatttc tggtaacggc aagtggattg acgctggtcg ggattggttc tgcgttctgg
      181 ggattaattg ccggaggcgt ttgttacgtg gtgttgaatt taatcgctga cagaaaccga
      241 tattgacatc ctccacgccc tgaaggacgt ggatccctgc tacgttcagg ctgtcgcctg
      301 aatcatttcg gtgggttcct gcttcaacgg gcggcctgac tgcaccatct ctccacaggc
      361 aagaacggcg tgccccgccg ctaaaatgtt acgagcacca tttacatcgg cgttcgctgt
      421 atatccacat acctggcatc tgaatttact ttgtgacagg cggttctctt tcgctgtatg
      481 accacagtac gcgcaacgct ggcttgtata cgctggcgga acagcaagca cctgaccgcc
      541 acgccagagc tgcttgtact caagctggcg gtgcatttca taccagccct gatccagtat
      601 cgaacggttt aaacctgatt ttgcccggac attgcgcccc ggctggctga ccgtacccgc
      661 tgctgacttt gacatgttgc tgaccttcaa atcctcaatg actatcattg cgtggttttt
      721 gctgacggcc gttgtgactt tgtgaaggta gtccctgcgg atatttgcga tacaggaatg
      781 cagtcgctgt attttgcgtt tctgcttctg ccagttgttg ctgaatttga ccttgcggct
      841 taactgtcgc tgaagtctcg ccagcgtctt ctggtttttc tgaaaactgt ttacaggctc
      901 aaagactgtg ccatctgaca gcgtggcgag tttagccacg ccagcatcca gcccgaccat
      961 tgatgctgaa gggtgagccg gagtggatac ttcactttct gtctgaatac tgatgtacca
     1021 cgtaccgcag gactggctga cagtgacatt tttcacaaca cccgtgacct gacggctatt
     1081 ccggtagcgc atccagccca gtttcggcag aaaaatacgg ctgttttcct gatcgagctt
     1141 aacgccctgc gggtagcgga atacatcatt ctgtccccgc tttttgaatc ggggaaaagc
     1201 agcccgcttc cggaagaagt ttttgtaagc ccgctcaagg tctttcagtg actgttgcaa
     1261 tggctgtgag ggagaatctt taagccattg cgtttcagtg gcatttttcc actcaaccag
     1321 ccaggaagcc attttcccgt aagggatgta tttattaccg gcctcatgat tctcattctg
     1381 acgtgccaga gcacgattga aaacgaaacg acatgcgcct gcgaagcgcc tcatttcaca
     1441 ctcctgttga ccacctggtc ttaactggaa tttaaatgcc tgtaatcgtt tcacaggtca
     1501 aatgatactt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc attgtgtttt
     1561 cctgatgcat gttcacctgg tctttgtcac tagataccga cgccagattt tttgacgcga
     1621 cagaaaaact acgcacttac ttttcaaatg tatgtgctta ttttgaagct gaactggttg
     1681 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctaacctccc aaactggcga
     1741 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac
     1801 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
     1861 gtagctgcgg aggtgtgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
     1921 ctggtcaggt ggaaaaccgc gccttatatc cccgccctga aggatggggt tttacggcgc
     1981 accggataaa aagcgccatc taacgatggc gcgtctggaa ctactttttg ttaacgtcaa
     2041 acatggtggc atcggtcgcc atgtcatcaa taacttgttt aatattttcg aaagccattg
     2101 gcgtactttc gttattcagg tctttacctt cgccctgacg aacgactttg ataaccggtt
     2161 tattagtcgc tgcatcaatc agctcacctt caaaatagag tcgagtatcc atggtacggt
     2221 ggcctgtagc catttgcgtc cccgcaacca ctaatgcaac aggaaccact tcatagaatt
     2281 gcagcccttc tttgctggta tctacaccgg taatggcccc acggaaaatc agactacgcg
     2341 gcccagcggt ggtaaccagt ggtttacgct gcgctatcgc ttctttcatt tcggtgttgg
     2401 tatagttcaa aattttatcc agaactttct gccctaccta ggtagaaggt tttggcaccg
     2461 gataataagt gattgggttc cagacgatgc tgtcatattt gctttgatca aaactcgggt
     2521 ctacccaacg taaaacaggt ttacctgttg ctgaggttgt ttcttttaaa tcagagtaat
     2581 tgtttaaaaa accagaatat ttatctggct gggtgatttt agaggcacaa ccagacagag
     2641 ccaacaagcc gcataaagct gcaactttcg caaatgatgt ggtacgcatg aatagtttcc
     2701 tg
//
LOCUS       DAETQN010000281         2690 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.287, whole genome shotgun sequence.
ACCESSION   DAETQN010000281 DAETQN010000000
VERSION     DAETQN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.287"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            230..769
                     /locus_tag="K0W68_004546"
     CDS             230..769
                     /locus_tag="K0W68_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBI3075883.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="K0W68_004547"
     CDS             complement(995..1318)
                     /locus_tag="K0W68_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HBI3075884.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="K0W68_004548"
     CDS             1604..1798
                     /locus_tag="K0W68_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBI3075885.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcaggga caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttggtagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAETQN010000282         2643 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.288, whole genome shotgun sequence.
ACCESSION   DAETQN010000282 DAETQN010000000
VERSION     DAETQN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.288"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            11..142
                     /locus_tag="K0W68_004549"
     CDS             11..142
                     /locus_tag="K0W68_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HBI3075886.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVERMGSWPEVVMA"
     gene            402..1397
                     /gene="pgl"
                     /locus_tag="K0W68_004550"
     CDS             402..1397
                     /gene="pgl"
                     /locus_tag="K0W68_004550"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBI3075887.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1438..2391)
                     /locus_tag="K0W68_004551"
     CDS             complement(1438..2391)
                     /locus_tag="K0W68_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3075888.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
BASE COUNT          673 a          588 c          647 g          735 t
ORIGIN      
        1 gggatttacc atgcttattg gctatgtacg cgtatcaaca aatgaccaga ataccgatct
       61 acaacgtaat gcgtttattt gtgccggaag tgagttgatt gttgagcgaa tgggaagttg
      121 gccagaagtt gttatggcat agtgtttacg gtcgttgata caggtaaaca aaagcgggga
      181 gagtcatact ccctgaatag tcgcgaaaat atctgtggca ttgtcaaatt cgtgtcggtg
      241 actaatattt tagtaatatg ccattcgaaa tggagtgcta acttattgat ttaattgatg
      301 tgcgatattg tattttatcg tgttttgttg tctgtttttt atccagtaag tgttgttatg
      361 tgtatgaaag ataagggatt tatcattttt gaggtgtgtt catgaagcaa acagtttata
      421 tcgccagccc tgagagccag caaattcacg tctggaacct gaatcatgaa ggcgcactga
      481 cgctgacaca ggttgtcgat gtgccggggc aggtgcagcc gatggtggtc agcccggaca
      541 aacgttatct ctatgttggt gttcgccctg agtttcgcgt cctggcgtat cgtattgccc
      601 cggacgatgg cgcactgacc tttgccgcag agtctgcgct gccgggtagt ccgacgcata
      661 tttccaccga tcaccagggg cagtttgtct ttgtaggttc ttacaatgcg ggtaacgtga
      721 gcgtaacgcg tctggaagat ggcctgccag tgggcgtcgt cgatgtggtc gaggggctgg
      781 acggttgcca ttccgccaat atctcaccgg acaaccgtac gctgtgggtt ccggcattaa
      841 agcaggatcg catttgcctg tttacggtca gcgatgatgg tcatctggtg gcgcaggacc
      901 ctgcggaagt gactaccgtt gaaggggccg gcccgcgtca tatggtattc catccaaacg
      961 aacaatatgc gtattgcgtc aatgagttaa acagctcagt ggatgtctgg gaactgaaag
     1021 atccacacgg caatattgaa tgtgtccaga cgctggatat gatgccggaa aacttctccg
     1081 acacctgttg ggcggctgat attcatatca ccccggatgg tcgtcattta tacgcctgcg
     1141 accgtaccgc cagtctgatt accgttttca gcgtttcgga agatggcagc gtgttgagta
     1201 aagaaggctt ccagccaacg gaaacccagc cgcgcggctt caatgttgat cacagcggca
     1261 agtatctgat tgtcgccggg caaaaatctc accacatctc ggtatacgaa attgttggcg
     1321 agcaggggct actgcatgaa aaaggccgct atgcggtcgg gcagggacca atgtgggtgg
     1381 tggttaacgc acactaaccg ctgatttacc cggcgcagtc tctcctgcgc cggtgtatta
     1441 acctatctcc tgtaacgcat gtctctggcg ttcgacgata ttggtccaca aattgtcttt
     1501 atcgtcagtc cacaaattaa tcagcaaggc aaaaaagcgt tctgctgccg gagaaagcac
     1561 ggcatctttg cggcgaataa tccccaatgt ccgacgtata acgggttcca ccagtggaat
     1621 accaataatc gaagaatagg gcgcatgggg cattgccaga ccaggaagcg ccgaaatccc
     1681 cagtcccgcc tctaccagtc ctaatgacgt cgaaagatga cgcacttcgt aaaaccagtc
     1741 cagcttccag ggcttgtcgg ccagctgttg ttctatcaac agtcggttgc cgctggagga
     1801 gcgtacgcca atcattttgt aacccactaa ctcctgccat tctacaagct gctttttggc
     1861 caatggatgg tcacgccgac aggccagcac gaacggctcg ttgaccagtg gggtaaaatc
     1921 aatggatgaa tttgtgacgt tgttcatgtt tatgccaaag tctgattcgt tacacagcac
     1981 cgattccatg caattattcg tgccttgttc cagaatccgc actttaatat tgggatacag
     2041 ctcattaaat ttaccgattg ccagcggtaa aaaataaaat actgcggtcg gaatacacgc
     2101 taacgtcacc ataccacgat gataagcatt catatcacga atattaaaaa gcgtttcatc
     2161 gaatttcttt attaattccc gcgcctccgg gagcaacctt tttcctgctt ttgttaacgt
     2221 aaccttgcga gttgtacgct caaatagttg aatatgtaaa tcttcctcca tcttttttat
     2281 tcttcgcgtt aatgcaggtt gcgtaatatt gagtaattta gcggcattgt taaacgaact
     2341 ggactctgcc agtatgacaa atgccttcat acttgataat tcatgcttca ttattactcc
     2401 ggaaaatgga agcgacgatt ttgggtggct ggccgttaaa aattttaact gcatttagcc
     2461 aacttaaatt aatgaaaaaa tgttattaat cgttgagcta aagtcattag agatgctttg
     2521 cccttaatgt aaccatatcg caataagtta tgtttttaaa ttgagggcat tattatgaaa
     2581 aaaataccct gggtaatgac tccaacttat tgatagtgtt ttatgttcag ataatgcccg
     2641 atg
//
LOCUS       DAETQN010000283         2608 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.289, whole genome shotgun sequence.
ACCESSION   DAETQN010000283 DAETQN010000000
VERSION     DAETQN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.289"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(132..848)
                     /locus_tag="K0W68_004552"
                     /pseudo
     CDS             complement(132..848)
                     /locus_tag="K0W68_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(901..1497)
                     /locus_tag="K0W68_004553"
     CDS             complement(901..1497)
                     /locus_tag="K0W68_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3075889.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1524..2126)
                     /gene="yadL"
                     /locus_tag="K0W68_004554"
     CDS             complement(1524..2126)
                     /gene="yadL"
                     /locus_tag="K0W68_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HBI3075890.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2141..>2608)
                     /locus_tag="K0W68_004555"
     CDS             complement(2141..>2608)
                     /locus_tag="K0W68_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3075891.1"
                     /translation="KATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQAD
                     FKLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDAT
                     FLKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          714 a          565 c          536 g          793 t
ORIGIN      
        1 gcgttcgagc attataacga atggcatccg catagtgcgc tgggttatcg ctcgccacgg
       61 gaatatctgc ggcagcgggc ttgtaatggg ttaagtgata acagatgtct ggaaatatag
      121 gggcaaatcc aaccttggcc tgcgccatag gctttaaatg aatacagata atttgatgag
      181 ctggataatt tgatctgttg agttactgtt ggatcaaagt ttgtatctgt atagaattca
      241 attgtaaggg tctgataaat accacctaca gcccagtatt tacgggtgct attcgcatta
      301 ttgcaactcc aatataacac gtcttcgctt ggattatacc acgaaaaata ttgttctgca
      361 gagtcaccaa tgtatatccc aggtgaacta acgttagtta cggtaccgta agctgaccac
      421 atgtttgaaa ttaataatgt gtaatacaaa ccaggaactg atgtattaaa taatttatgg
      481 gagccataca ttttacctga gtcaaccatt gcattttcaa tacgcattgc gctaccactt
      541 gtgctatcac ttctgttgca ataaagaaaa ccaccaccag aagcggtatc taactgactc
      601 tactgtaatt ttacacctgt aggtccaagc tgcgtcggtt tgtagaaaac taaattattt
      661 gtacttgttg catatctgaa tatgacagta gcatcgccat tcggccccgt ttctctccat
      721 ttttgatctg acggaccaac gccgtaacta ttcccaacaa tatatcctac gttatcatca
      781 gtgccagcac tcgcggtata acaactcaag cacagaatta aaaaaaaatg tctgaagaat
      841 ttcttcatta tcactccacc atctactgct gtaacagtcg cagttatttg ttttgatcta
      901 ttattcgtag tacacatcaa ctaatatatg actcattact tggccagata tgacatccag
      961 cgcgggatcg attttttgca ttctggcata aaatttccat gaagaattat gcagggcaga
     1021 agtcattact ggatacacgg aacgagaatt tcctgctgaa ctaataacat tgaatatatt
     1081 ggtcgggtcc tgaatggaaa agagaacgac accgacattt ttagccccgg ttgcgacttc
     1141 attggcaaat atttgccttg aacctgcagc catgacaccg ctaagggctc taaaatcaat
     1201 atgtaactga ttaagcacat cgccagttcc ctgtaatgca cagtctgata ctgtgacagt
     1261 gaactctttt ccacctggtt gatagctttc aggagtaaca ttatctgaaa aatatcccac
     1321 cccaacgacg ccaagatcaa tattaccctg gttactgatt ccgcttttgc aagtgctgtt
     1381 ttttacattg gcaacaaggt caatatcctg cccagcgatg gctggatagg taatgggaaa
     1441 ctgtagtgac agaaacagaa ttattctctt catcagctta cgttgagtgg gatgcatata
     1501 tagttcctgg atctgtcagt ctgttagtta taggttactg taaatgtggc aggtgcagaa
     1561 aatttacccg cagtgacatt gcttacggtt ttattttttt cgacgaccag tcgagcactc
     1621 atcggataat aaacaaccgc gttggctgca ttggagatag tgacctcttg agatgctggt
     1681 gtcacacaac taaattgtgt tgcgctccct gttgccggag tttcggtgct ccagacttca
     1741 acggcgacgg cacttgcttt atctgcggct gtggatccat ttgcgagccc cgcaccgtca
     1801 ttagcagtac cttcgcatgt ggctcgcttg gttaatttta tttgtgcttt tttattgggg
     1861 atacccgcac agtctttgaa tttgagtttg aatgttttaa ctttggtctt ggcattgatt
     1921 tcagaaatat atacatcgcc aaagtcaaca acagaaatgt cctgatcaga gtcatttact
     1981 aatgtagcgg tacaagtacc tatttctacg gtagtaatca cggtcagccc aatgctgtct
     2041 gttgccgcat aagagggtac gctgaacaat gaggcagcca aaacgacact gctggataat
     2101 ccataacgta aatttttaaa tgtcatctta attatccttc ttattgatag atgaaattaa
     2161 acgttgccag tgcacgaaaa ttccccggta cgccttgccc tgcatctgtt tcacgtagcg
     2221 caacggtcat ttcaagaccc ttatcgggct gcatctcgtc tgtgctccag cgtatctttt
     2281 ccgcactgtt aggtttaagg aaagtggcat catcagtagt ccgttttttg aaacccatac
     2341 cgatattact tgtcgtcgaa gatgaatcac cagactgcgg tataataagc ttaggtgagc
     2401 ttgatgatgc atttccggtc agagtggtat caatccaact gatgccactg ctgcacccac
     2461 tagcaaccag tttaaaatca gcctgagatt ctgtcgtttt attcgcgatc ttatccagac
     2521 ccatcttagg gattctcaat gtgtagttat tattgccatc attcgtgacg ttattaccag
     2581 taagtgtgat gttacaggtt gtcgcttt
//
LOCUS       DAETQN010000284         2557 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.290, whole genome shotgun sequence.
ACCESSION   DAETQN010000284 DAETQN010000000
VERSION     DAETQN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.290"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(59..262)
                     /gene="tatE"
                     /locus_tag="K0W68_004556"
     CDS             complement(59..262)
                     /gene="tatE"
                     /locus_tag="K0W68_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBI3075892.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(391..1179)
                     /locus_tag="K0W68_004557"
     CDS             complement(391..1179)
                     /locus_tag="K0W68_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBI3075893.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1272..1655
                     /gene="crcB"
                     /locus_tag="K0W68_004558"
     CDS             1272..1655
                     /gene="crcB"
                     /locus_tag="K0W68_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBI3075894.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1709..1918)
                     /gene="cspE"
                     /locus_tag="K0W68_004559"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1709..1918)
                     /gene="cspE"
                     /locus_tag="K0W68_004559"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBI3075895.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2093..>2557)
                     /gene="pagP"
                     /locus_tag="K0W68_004560"
     CDS             complement(2093..>2557)
                     /gene="pagP"
                     /locus_tag="K0W68_004560"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HBI3075896.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          652 a          659 c          580 g          666 t
ORIGIN      
        1 ttttgaagcg gtttttttgt atcgaacaag atattgaggg agcgtcctgc tcgccacgtc
       61 actctttatg agagagcttt tcagcctgaa gatcaacgtc tgcgcctttt ttcgccgcag
      121 catcgtcatc attcatcgcc ttcttgaacc ctttaatggc cgctccaagg tctccgccca
      181 gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag cgccgcaact accagcagtt
      241 tggtaatact aatctccccc atagatacct tcttgacata taaccggcaa cagaacgcgc
      301 cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc acacaatagc aatctgtaac
      361 aaggtgaatc aagcgccgag taaaaaaaca tcataataat tgcggcggcg caaagcgacg
      421 gttgtttaag acgggcagtt gcgcgcgcac ctgacgcaca cgttcgggcg tcacttccgc
      481 cataatgagt gcaggcattt ctgacgctgc cgcaatggtg acgccaaagg gatctataat
      541 ccggctttgc ccgatatttt tgttcccgca ctcccccgcc gccaccatat aacaggtggt
      601 atccagcgca cgagcggcaa gcaacgttga ccagtgatgc tctttgagcg gcccgcgaac
      661 ccaggcggca ggaagtacca ggatttcagc tccctgtaat gcctgtgcca gcgccagctc
      721 tggaaagcgt aagtcataac aggtcatcag accgaccttc atcccctcca cctcgagtaa
      781 cggagcgatt tcattaccag catcaacacg gcgtgattcc tgaatggcaa atgcatcata
      841 gagatgcaat ttggcataac gggcgacgat gtttcctgcc tgtagcgcca ccagcatatt
      901 ccatgcgcgc cccggcgttg aaggaacatg aatcgtcaga attgtcgtca tcatgttacg
      961 tttactttct cgccgtaaac gtccgaggaa ttcgccttcc agcagctgtg ctgatttaac
     1021 cgatagatct gcatcatgat catcgcgcgc cagcaatgct tccggcagga cgaacagcga
     1081 tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa atctcagcgt tcttttccca
     1141 cacagatgta acagcaaact gtcctgcggc aaccagcatt gccacctcct gtcagagtcg
     1201 taaaattcgc caacgacgat tattcggcgt tacacttatc actcatacaa atcaaatagc
     1261 aggattttgc agtgttacaa cttcttttag cagtttttat tggcggtggt acgggaagcg
     1321 tggcgagatg gctgttaagt atgcgattta acccgctgca tcaggcgatt ccgttgggga
     1381 cgctggcagc aaacctgatt ggggcattca tcataggaat gggattcgcc tggttcagca
     1441 ggatgacgaa cattgatcca gtgtggaaag tattaatcac caccggattt tgtggcggtc
     1501 taacaacctt ctcaacattt tcggcagaag tggtgttttt gttacaagag ggccgctttg
     1561 gctgggcgtt actgaacgtt ttcgtcaacc ttctggggtc ttttgccatg accgcactgg
     1621 ctttctggct gttttcggcc tcaaccgcac actaaagacc aaaaaaaacc cgctgattaa
     1681 gcgggttttg aattcttgct gacgtatctt acagagcgat tacgtttgca gcagaagggc
     1741 ctttggcacc gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga gttttaaaac
     1801 cattagtctg tattgcagag aagtgtacga acacgtcttt gctgccgtct tccggagtaa
     1861 tgaaaccgaa tcctttggac tcattaaacc acttaacgtt acctttaatc ttagacatca
     1921 aaattacctt tacatgaaaa atagacacaa atgctgtgtc ggttaccagt acaccaattg
     1981 tggtacgctt tgtccagtgc aactttacta aaaagtgata aaagtcgcgt tatttttgat
     2041 gttacttatt ttagttattg attttaaaga agttactaaa acttcatttg tctcaaaact
     2101 gaaagcgcat ccaggcaaag tacacattgc cattgttgta ggtacccgga atgtaggtca
     2161 tctgaaaagt cactgggcca taacccacgg aggccaatgg cagtagaacc gggagaggga
     2221 tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa tcccagacct aaatgaaaat
     2281 tttcatccgc cagcggtcgc caggtacttt cccatccgta tccggcaatc ggttcccatt
     2341 tgttccacga gtccttaaat gccatggcat acaggccatg ccagtttcct ttttcatccc
     2401 atcgcgactg accaaaaccg ccaccccacg gtcgctcgtt atagcgatcg gttttttctt
     2461 tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat atataaatca taatgttcag
     2521 gctgttgcca ggtttgtgca atattttctc taaacgt
//
LOCUS       DAETQN010000285         2539 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.292, whole genome shotgun sequence.
ACCESSION   DAETQN010000285 DAETQN010000000
VERSION     DAETQN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.292"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(107..1525)
                     /gene="phrB"
                     /locus_tag="K0W68_004561"
                     /pseudo
     CDS             complement(107..1525)
                     /gene="phrB"
                     /locus_tag="K0W68_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
     gene            complement(1522..2031)
                     /locus_tag="K0W68_004562"
     CDS             complement(1522..2031)
                     /locus_tag="K0W68_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBI3075897.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          642 a          658 c          579 g          660 t
ORIGIN      
        1 caggcctacg tatttcctgc aatttattga atttgcacaa atttgtaggc tggataaggc
       61 gttcacgccg catccggcat ctgggctcga ttgcctgacg cgtctgttat ttccccttcc
      121 gcgccgcctc atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg actatcggtt
      181 gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca tgcaccgatt
      241 tccccggtac attgcgtagt tccggtagcc actggcggat aaactcacct tcgagatcaa
      301 atttctcgcc ctgggttgtc gggttgaaaa tacgaaaata cggcgctgca tcggttccgg
      361 ttgaagcggc ccactgccag ccaccgttat tggctgccaa atcaccatca atcagctgcg
      421 acatgaaata tcgctcgcct tcgcgccagt cgatcaataa atctttcacc agaaaactgg
      481 ctgtaatcat ccgtagcctg ttatgcatcc agccagtgct gttaagctga cgcatagcgg
      541 catcaacaat cgggtatccc gttttgcctt cctgccaggc ctgtaaatgt gcgggattgc
      601 tctgccactg tacacgatcc gtctaggcaa taattggacg atgtttacac aacgaggggt
      661 gatacgttat caggtgacgg taaaactcgc gccagatcag ctcattaagc cagacactac
      721 cgaccccacc gtccagcgcc tgcggctgtt cagccaacaa gcgatgcaag cactggcgag
      781 gcgataaccc gcccgttgcc aggctggccg acaaacggct ggtgccttcc actgccggaa
      841 aatctcgttg ttgctcatat tctccggcac cgttctggca aaactggcgt aattgcgcaa
      901 tcgccgcttt ttcttccacc ggaaaatgcg cagtatcgaa agactgacga ggataattca
      961 gcgtaatgga tggcgagggc tctatcgatc cgctactacg aacttttggc gcagcgacgc
     1021 actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt
     1081 tgtacatctc gtgattaccg gtcatcaccg cgccaggcgg caggatcacg ctgtcatcaa
     1141 atccttcaca caccacgtta cgcagcgctc tttcaacttc cacatcccgc gcccgctcat
     1201 tcacttcata ctgatagtta taaaacaggt gggtaacgct gttttccgcg cacacctgtt
     1261 taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt
     1321 tttccgcaag cgctatttgt agcccattca gttgagcatt gataagttca gcctgacgcg
     1381 gcgacatgtt atgcgtcgcc cactggcgtg gtgtagcgat atacaacgcc agcacgcgtg
     1441 cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt aaatcttggc
     1501 gaaaccagac cagatgggta gtcataaaac tcctgaatgg cgcaagttaa tcacacgcgg
     1561 ccaaagttcg aaataacgct gccctgaaag ccaggcgtca tgataaagcg ccatatagtg
     1621 tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac tgtcgataag
     1681 cgcagccagc tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg
     1741 catcaaaaca ttggtgtgat cgcgcgggtt ggcggggtga gaaagcaaaa caactacgcg
     1801 ttggcggtac tggttgtaat agtcatccag attttgccac tggtgaatat cagccacgaa
     1861 cgggccaagt ttgcggtact ccggctgaga atgcgccaga aagacgagtt tatagcggct
     1921 gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg
     1981 aagggcaaag atacggatta gggtgctgtc atcaaatcgt tgtagattca ttactgctcc
     2041 tgaacggtat aaaggagtaa gtgtagacga tggtaaatga attttatggg gcagagtttt
     2101 tgcggggaag tattttgttg gtaaaaatat tatcaacttc atctgagtga gaattattct
     2161 actatttccc gagctgcatt cattttttgt attttttatt tttagaaaat aacagttctc
     2221 aaagcttaat tacctcacta ttaattgtat ttcaaactaa caatcgcact ccattttctc
     2281 tttttaaaaa acataaacac tgttaaaaca aatacttatt tcgttatctt aaatgctatt
     2341 tctacatcac tcgtccttat aatcaaatta ttcttattca tagctatttt tgcaggttta
     2401 aaatagcggc tttcaccaaa gtcagccatg aaaattagac tacaagtcag ggcaagatta
     2461 cgaaagcccg ctccccgcaa ggactgacgc cagatagttt ctgtccatgg ctgcttttcg
     2521 catcttacgt cttaaccct
//
LOCUS       DAETQN010000286         2446 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.293, whole genome shotgun sequence.
ACCESSION   DAETQN010000286 DAETQN010000000
VERSION     DAETQN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.293"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(25..315)
                     /locus_tag="K0W68_004563"
     CDS             complement(25..315)
                     /locus_tag="K0W68_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBI3075898.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="K0W68_004564"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="K0W68_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBI3075899.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="K0W68_004565"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="K0W68_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBI3075900.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="K0W68_004566"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="K0W68_004566"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2446)
                     /locus_tag="K0W68_004567"
     CDS             complement(2171..>2446)
                     /locus_tag="K0W68_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBI3075901.1"
                     /translation="GRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          667 a          655 c          549 g          575 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat
     2341 cggcatcacc ccacgccata tgcgtttgtg ctgtgacaga ttttgccagc atgactaccg
     2401 gggacaggcg gcccagtgag tcggggccag cattccagat acgacc
//
LOCUS       DAETQN010000287         2438 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.294, whole genome shotgun sequence.
ACCESSION   DAETQN010000287 DAETQN010000000
VERSION     DAETQN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.294"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(443..519)
                     /locus_tag="K0W68_004568"
     tRNA            complement(443..519)
                     /locus_tag="K0W68_004568"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(483..485),aa:Arg,seq:tct)
     gene            complement(533..609)
                     /locus_tag="K0W68_004569"
     tRNA            complement(533..609)
                     /locus_tag="K0W68_004569"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(573..575),aa:Arg,seq:tcg)
     gene            complement(617..692)
                     /locus_tag="K0W68_004570"
     tRNA            complement(617..692)
                     /locus_tag="K0W68_004570"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(657..659),aa:Ile,seq:cat)
     gene            complement(733..1791)
                     /locus_tag="K0W68_004571"
     CDS             complement(733..1791)
                     /locus_tag="K0W68_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HBI3075902.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1899..2139)
                     /locus_tag="K0W68_004572"
                     /pseudo
     CDS             complement(1899..2139)
                     /locus_tag="K0W68_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2364..>2438)
                     /locus_tag="K0W68_004573"
     CDS             complement(2364..>2438)
                     /locus_tag="K0W68_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBI3075903.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          586 a          698 c          560 g          594 t
ORIGIN      
        1 atagcggtca tgtgatgccg gtttcccggt aactcagcac cggtatctga gtcaacgttt
       61 tctctactgg gtcatttccg atacgccctg cctgctgaca ggctttcatc acatctgaaa
      121 atatagcacc ctgactgata ctgtagtacc taaggttcca gaaactgtga tgtatccggc
      181 acagaaaagc ccctccggag agggctggag agtggcgcta tgtgccattg catgatgccg
      241 ggtgcctccc ggtgaattca gtaccagcac ctgaatccgc gattatccca tatacctact
      301 cgctgattgc ccctccgcac agggggattc accatgccag tttcttttaa caaactcccc
      361 gcaaaacaga caactgtcaa ccgtctgaat tgtgagacat ttaaaaaaaa ggcccgcaaa
      421 agcgagccag ggaaaataag tgtggcgcgt tgtactggat tcgaaccagt gaccgattgc
      481 ttagaaggca attgctctgt ccggctgagc taacaacgca tgatgcagat aatggaccgc
      541 catcggggac ttgaaccccg cgcagccagc ttcgaaggct ggcgctcttt cctgatgagc
      601 taatggcggt atgtgatggt ggcccttgct ggatttgaac cagcgacctg gcgattatga
      661 gtcgctcgct ctcaccactg agctaaaggg ccgggagccg cataataacg acgcgtaatt
      721 aattcttcaa tatcatccgt tctggctgac taaatcctgt acttcccgaa ccgtctgctc
      781 aaaacgttca gtctccagct caacgccaat tgcacgacgc ccgagcgcca gtgccgcttt
      841 cactgtcgaa cccaacccca tgaaaaaatc tgcaatcagg tcacccggac gactgctagc
      901 gctgattatc tgctgcagca tttctgccgg tttttcgcac ggatgtttcc cgggatagaa
      961 ctgcaccggt ttatgcgtcc acacatcggt atacggcacc tgcaccgtca cgccaaaata
     1021 ccgccgcaga tgtttatatt cactcagcag ctccgcatac tgccggttca gtgaggtata
     1081 cgtatccacc agctggtggt ggagcttttc cagttcacca cgccgatgct tctcttctgc
     1141 cacccgggca aacagcgcct gtaatttcag ataatcgctt tcattcggca actgccactg
     1201 accggcactg aaccagtgcg acaccatgtt tttctttcct gtggcatctg caatctgttt
     1261 tgccgttatc cccagggcag ctcgcgcatc acgaaagtaa gaaatcagcg gggccatcac
     1321 atgctgtttc agtgcactgc ccttcgccgc atacccggca tctttcggac gatacggccc
     1381 ctgataatgt tccgcgaaca gaatgcgctc tgtggcgggg aaatacgccc gcaggctttc
     1441 cttgttgcat ccgttccagc gtccggacgg cttcgcccag ataatatggt tcagcacact
     1501 gaagcgttca cgcatcatga tttcgatatc agatgccagg cgatgaccac agaacaggta
     1561 aagacttccg gcaggtttca gcacccgcca gaactgcgcc agacactggt ccagccactt
     1621 caggtaatca tcgtcgccct tccactggtt atcccagccc tcaggcttca ctttaaagta
     1681 cggcgggtcc gtgactatca ggtcaacaga attttcgggt aacgaccgga taaattccag
     1741 gcagtcggcg ttgattaact cacaactgga tatttttaca gtattaagca tggatcatta
     1801 agcctgtctc tgataggctc attctgcttt tgcgcaaagc agtgggcctg aggtttgctt
     1861 gtgaacccaa cgcatgagca gatggctggt gggtgcccct aacacccacc agccgcccat
     1921 ttaccacaaa taaaaaagcc ttcactgcgg aaggcgtctg taacaaccga actgatagtc
     1981 tgccagaccc gccataacca gctgggtcag tattaactgg cagcgttcgc gtgaaaggta
     2041 agtattctgc gctatctccc cgactgtcgc cggttcggta acgcttaatt cattaaacac
     2101 cactctggcg gtttctgtca tatcctgctg ttttagcatg tcttttcctt ttccggttaa
     2161 cgtgacacac caataactct tgtcgaaaaa gccagcaagc tgaaagacag gtattcaccg
     2221 ccaccagcgc gtttactgta ctgacgcgat ttcagtcata aaaaacccgc tcggcggcgg
     2281 gttgtagaaa ctcttctaac gtcaggcata aaacgcccat cgttatgacg aatttaccac
     2341 agattccgga aaaatcaacc ttgttaccta gttacctttt ttaactgccg ctcagcccat
     2401 gcttcttcaa tatcaaaccg ggtcaccagc gcatcata
//
LOCUS       DAETQN010000288         2403 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.295, whole genome shotgun sequence.
ACCESSION   DAETQN010000288 DAETQN010000000
VERSION     DAETQN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.295"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            376..642
                     /gene="hipB"
                     /locus_tag="K0W68_004574"
     CDS             376..642
                     /gene="hipB"
                     /locus_tag="K0W68_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HBI3075904.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            642..1963
                     /gene="hipA"
                     /locus_tag="K0W68_004575"
                     /pseudo
     CDS             642..1963
                     /gene="hipA"
                     /locus_tag="K0W68_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2147..2248
                     /locus_tag="K0W68_004576"
                     /pseudo
     CDS             2147..2248
                     /locus_tag="K0W68_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          666 a          505 c          619 g          613 t
ORIGIN      
        1 gagatttggc gcagtggagt agcaacgcgc aggatcgctg gcatcttggc gtgatggcag
       61 gctacgccaa tcagcattag cctgtggggg aatgtcggtg tgcaactagg tgataaaggc
      121 tatagcgata ctcagggcat gctgggagtg aaatatagct ggtaaaccgt ataagccgca
      181 tgtcgagatg gcatgcggct taatattgcc aacttcaaaa ggcttttaaa gcctcccttt
      241 tatccgcgat cgcggatatc gcagcgttta tcccgtacag cggataagat gtgtttccag
      301 attgacttta tcctcactaa aggataaaac tgataatatc cccttaagcg gataaatttg
      361 ctgtggacgt atgacatgat gagctttcag aagatctata gcccaacgca attggcgaat
      421 gcaatgaaac tggttcgcca gcaaaatggc tggacgcaga gcgagctggc gaaaaaaatt
      481 ggcattaagc aggcgacgat ttccaatttc gaaaacaacc ctgacaatac cacgctgacg
      541 acctttttta agattttaca gtcgcttgaa ctctcaatga cgctatgcga cgcgaaaaat
      601 gcctcgccag aagcaacaga acagcaagat ctggagtggt aatgcctaaa cttgtcactt
      661 ggatgaacaa ccagcgggta ggcgagttaa cgaagttagc caacggcgcg cacaccttta
      721 agtatgcacc ggagtggtta gccagccgtt atgccagacc gttgtcactt tcgctgccat
      781 tgcagagggg gaatatcacc tctgatgccg tatttaactt cttcgataac ctgttacccg
      841 atagcccgat tgtacgtgac cggatcgtta aacgttatca tgccaaatcc agacaaccgt
      901 ttgatttgtt gtcagaaata gggcgagaca gcgttggtgc cgtgacgtta atacccgaag
      961 acgaaaccgt aacgcgtccg ataatggcat gggaaaagct tactgaagcc agacttgaag
     1021 aagtattaac ggcttataaa gcagatatcc cgctaggcat gattagagaa gaaaatgact
     1081 ttcgcatctc ggttgctggc gcacaggaga agacagcact gctcagaata ggcaatgact
     1141 ggtgcattcc gaaaggaata acgccgacga cgcacatcat taaattaccg attggcgaaa
     1201 tcaggcagcc caatgcgacg ctcgatctca gccaaagcgt tgataatgag tattactgtc
     1261 tgctgctggc gaaagaactt gggttgaatg ttccggacgc agaaatcatt aaagcgggaa
     1321 gggtgcgcgc gttagcggtc gaacgttttg acaggcgttg gaatactgag cgaacggttt
     1381 tacttcgctt gccacaggag gatatgtgtc agacattcgg tttaccttca tcggtgaaat
     1441 atgaatcaga tggaggccca ggcatcgcgc ggatcatggc gtttttgatg gggtccagcg
     1501 aggcgctgaa agatcgctat gattttatga agtttcaagt gttccagtgg ttgattggcg
     1561 caacggatgg ccatgcaaaa aacttctccg tatttattca ggctggcggc agttatcgac
     1621 tcacgccatt ttacgacatc atttcagcat ttccggtcct tggcggtacg ggaataacat
     1681 cagcgatctc aaactggcaa tggggcttaa cgcatccaaa ggcaaaaaaa cggcaatcga
     1741 taaaatttat ccgcgacatt ttttggcgac agcaaaggtg ctgagattcc cggaagtgca
     1801 gatgcatgaa atcctaagtg actttgccag aatgattccg gcagcactgg ataacgtgaa
     1861 gacttcatta ccgacagatt ttccggagaa cgtggtgacg gcagttgaaa ccaatgtgtt
     1921 gaggttgcat ggacggttaa gccgagaata cggtagtaag tgagatatgg ggtttggtcg
     1981 ttgttaatga gcaatcatga ccggccaacg actggattct cccttggttt atgtttgtgc
     2041 gcataatgta tattatgtta aatcatgtct gagtgatgcg aagtgctttt ctggtagtcg
     2101 ttattcgttc agattaggtg tccttgtgat ttaaagatgt tatcgtggca cgaatatact
     2161 gccattccag gctcgttata ttgctctcgg gcagtccgta gctggtactg caaacgctga
     2221 tgcgaccttc aaagttcaat atctataatt caatcttaag gtggctatac gtcatctgaa
     2281 attgaaacag ggtgtgtgtg gcatggctac gcacacccga gcaattcacc aatgtgaatc
     2341 tcattattaa tgaggagtgg tttgtatttg gagcgctatg aaaaatcaca taatatctaa
     2401 act
//
LOCUS       DAETQN010000289         2233 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.296, whole genome shotgun sequence.
ACCESSION   DAETQN010000289 DAETQN010000000
VERSION     DAETQN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.296"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..926)
                     /gene="ospC4"
                     /locus_tag="K0W68_004577"
     CDS             complement(<1..926)
                     /gene="ospC4"
                     /locus_tag="K0W68_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HBI3075905.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNILRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQGFREFCYNK"
     gene            1342..1866
                     /locus_tag="K0W68_004578"
                     /pseudo
     CDS             1342..1866
                     /locus_tag="K0W68_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1949..2221
                     /locus_tag="K0W68_004579"
     CDS             1949..2221
                     /locus_tag="K0W68_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075906.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          645 a          465 c          439 g          684 t
ORIGIN      
        1 tttttgttat aacaaaactc tctgaatcct tgatctttac tctttcttat aaaatcaata
       61 agatgcaacc caattccaag cctcatatct ttagtgttaa atattggaac atcagttttc
      121 ccctgattac catgcacata cctgctaact tcatccacaa cctctttaaa gttatctaag
      181 aaaaatgatt ggtgctcatg gtaaaaaaca ggtggaatag cattatcaaa gtgatcggtt
      241 aatgtcatat atccatatgg agagtcatga tcaatgatat acgcattggc gccaaaatct
      301 acagtatgat gtgttttatt tagtgggagt ttttcttgat gattggaaat ttcaacccca
      361 aaaaaaacaa aatcgtggtt agagatttct ttaatatcct cagagtatgt attcttatgt
      421 gtttgtatat tttcagactg aattttatca aaggatttaa tattaagaat attatttgac
      481 agattagagt tactttgatg tctgaagtta aaattagaat taataataat agaaagtaag
      541 tgacgttcgt ctgaagaaag aggatgaact ttttgtatta tctcagagat atagtgtctg
      601 gacaccttat cgagatgctc aagtgaccga aatgatgacc gtgctccaga tggcgcttca
      661 atagaaagat ttagtgattt gaaactggtc ccttgagaaa acattttgct ataactttgc
      721 gctgctatag acttccttaa aaagtttttc acagtattag cacagtgatc taaatgtttt
      781 tcacttatgc cagaagaaga tgagtttgtt acgcgtaata tatttttttg taatgctttt
      841 gttgctttat tcggattcgt ttttccatca acaagatcaa tattattctg aacattaata
      901 tgatttactg cttcaggtat tttcatcccc cccccaatta tgtgcgtttt tatctgtggt
      961 tatttagata acattatcta actggacact ttcttgtatg tattatagct aaatgaacag
     1021 attttaatat gttgtatggt tttcactctg aaatcccctg aatttaatca cgtttccttt
     1081 actgaaggca attttaaccg tagtatcaat tattaaccct tgggcttgat atccctctcc
     1141 tcttctgggc acgggggatg tcagattact tattacagaa accaaaagaa ttgtaagcgt
     1201 catctcagtg tcgtctattt caatgtctga tgattggcga tgatagagtc ctcttataag
     1261 taagcacccc atcagcgacg tcttgtgaaa attgtcctgt ctggcaacaa tcgcgcccat
     1321 ctatattgat ggacacgaac gatgaattcc cagacaacaa aagatattcc ctgcttccgt
     1381 tcttatttgc ctgatgccct gcgtttaaga tttgaagata aactgaccat ccgggccatc
     1441 gctcagcgtc tgggtctcag tcattccaca atacatacgc tttttcagcg atttcttgca
     1501 tccggtatcg catggccatt gcccgattca gtttcattcg ctcaacttga cgccatcctt
     1561 tatgccaaca gaaagaagga attaacagag cctcaaatca gagaaggctc atggcgaaaa
     1621 gaacggcgaa ccagctatag ccgtgaattt aaggtccgtc tggctaagca ggcattacag
     1681 cctggtgctg ttgttgcccg gatcgccaga gaacacgata tcaatgataa cctgctgttt
     1741 aaatggaaaa gccagtacga ggacggctta ctgagcgatg atgacataca ggaatgcatg
     1801 cctgtcccgg tggcactgac tgatacgccg gagccgacca gaccagttac aaatcccttc
     1861 tggcgtaaga accacggcag cctggcggct gcaaatcggg gtgtggcaga atatgagctt
     1921 tcagagtaac catccagaat gagttcccat gtcacacaac ctggagcacc agaaagtcca
     1981 caccagaatg gtgaaagagg ttctgaaagc cgtcgccaga gccaataatc acccttatca
     2041 gtccgtattt acagacttta tcgccggtca tccctcctgc acggtctgtt tctgggaaac
     2101 cttccacaaa atgtacccgg attcaccgta cgaatacgtc acgttctgcc atacctgccg
     2161 tcgttttgat ttatacgaaa cagaagcgga gatgaaggcg gatgacccga agtggtggtg
     2221 attttcagcc ccg
//
LOCUS       DAETQN010000290         2095 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.297, whole genome shotgun sequence.
ACCESSION   DAETQN010000290 DAETQN010000000
VERSION     DAETQN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.297"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..125)
                     /locus_tag="K0W68_004580"
     CDS             complement(<1..125)
                     /locus_tag="K0W68_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBI3075907.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYT"
     gene            386..721
                     /gene="iraM"
                     /locus_tag="K0W68_004581"
     CDS             386..721
                     /gene="iraM"
                     /locus_tag="K0W68_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBI3075908.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1002..1133)
                     /locus_tag="K0W68_004582"
     CDS             complement(1002..1133)
                     /locus_tag="K0W68_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HBI3075909.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1633..1709)
                     /locus_tag="K0W68_004583"
     tRNA            complement(1633..1709)
                     /locus_tag="K0W68_004583"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1673..1675),aa:Arg,seq:tct)
     gene            complement(1810..1885)
                     /locus_tag="K0W68_004584"
     tRNA            complement(1810..1885)
                     /locus_tag="K0W68_004584"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1850..1852),aa:Ile,seq:cat)
     gene            complement(1988..>2095)
                     /locus_tag="K0W68_004585"
     CDS             complement(1988..>2095)
                     /locus_tag="K0W68_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBI3075910.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          677 a          521 c          371 g          526 t
ORIGIN      
        1 gtataagggg ttatggccac cggactacca aacggctgcc agccctcttt cagtttatgt
       61 gtcagctttt ccgcaagatc tgacggcggc gccgccctga caacatcata gtgtttaaat
      121 gccatggttc tttccaccat ctgaaaaata attctttaaa atacctgaca tgtaatacag
      181 aaaaaacaca aaaccatacc ttaaataaaa acctgattat caagcagata tgcatggata
      241 aactacaaga cgagatataa accaccctgc atttaaataa acaataaaca acatcagaaa
      301 aataattctg ctctatggtt tacattcaaa aatatcattt atacttttca gaacatcacc
      361 agcaaggcat aaacaaggaa actaaatgaa gtggattgtg attgatacag ttatccagcc
      421 atcatgcgga atatcttttt cagtcatatg gagtaaaata aaattaataa tctggtatca
      481 atcggatgct ttcttacctc ctgaaagtat atttacactg actcacacag gcatcatgct
      541 caataacaaa gtgctacctg taaccattta caacgtagta ccattcaata aaacattctg
      601 gaattcaatc aaaaatagcc aggaatgccc tacaaataca gataacgtat tgaatgaatg
      661 ctttaataac cgttgcactc tgcaaatatg tccttatgga ctaaaacaac aaagtccata
      721 aggagtttac tcacatctga caaaatcaat ataaacagcc cctccggaga ggggctggag
      781 agtggcgcta tgtgccattg catggtgccg ggtgcctccc ggtgaattca gtaccagcac
      841 ctgaatccgc gattatccca tatacctact cgctgattgc ccctccgcac agggggattc
      901 accatgccag tttcttttaa caaactcccc gcaaaccaga caacagtcaa ccgcctgaat
      961 tgtgaagtat ttaaaaattt ctcccgctaa ctgatacccg gctaacagtc tggcgttttc
     1021 tttttcagca acgggaaagc aacaaccacc acacccgcca ccagcacacc gtcagccagc
     1081 actgacatta tccggctgct gcaatgccat tcacaaaaac agtaagcaat cactttttac
     1141 cgtaacaggt gataatccag atatgtatct accccagatg agtaatccga agttcatcca
     1201 taccacaggt cctggctatt ctgttgtact cctgaacaag agcaaataat tctgaattag
     1261 caaccatgaa ctcatcgcaa accctctgta tagcatcact attcagaata ataacgtctc
     1321 ttcccgaaag acgatcagga gtacagaaca aaactgtcaa acggctgaag gcctttgctc
     1381 gtgctgcatt gactatatca atacgctgcc taaggatgaa acaccccgac gcctcatcaa
     1441 tattcactct acccacacca tatgaatgat aaatatttaa tgctgaaaaa accattagac
     1501 cgtataacaa acactcaatc aatacttaac agaactttta tttttgacaa acataaaata
     1561 ttttcaacaa tatcctgagc caggtatatt tcagtataag gctctgccgg aaggaatctg
     1621 gaagaatgaa tatggcgcgc tgtactggat tcgaaccagt gaccgattgc ttagaaggca
     1681 attgctctgt ccggctgagc taacaacgct gaataccgat aatggaccgc catcggggac
     1741 ccgcccccgc accaacaacc ctgttatcgt gtcgtctgct cttcctgata agctaatggc
     1801 ggtttgtgat ggtggccctt gctggatttg aaccagcgac ttggcgatta tgagtcgctc
     1861 gctctcacca ctgagctaaa gggccgggag cagaataata acggtccgta attaattccg
     1921 caataaaaaa cccgctcggc ggcgggttgt agaaactctt ctaacgtcag gcataaaacg
     1981 cccatagtta tgacgaattt accacagatt ccggaaaaat caacctagtt acctttttta
     2041 actgccgctc agcccatgct tcttcaatat caaaccgagt caccagcgca tcata
//
LOCUS       DAETQN010000291         2089 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.298, whole genome shotgun sequence.
ACCESSION   DAETQN010000291 DAETQN010000000
VERSION     DAETQN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.298"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(60..1025)
                     /locus_tag="K0W68_004586"
     CDS             complement(60..1025)
                     /locus_tag="K0W68_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBI3075911.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQIAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="K0W68_004587"
     CDS             complement(1879..>2089)
                     /locus_tag="K0W68_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075912.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          549 c          437 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcaatc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag acttgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcactccc gaacggctga gcccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAETQN010000292         2080 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.299, whole genome shotgun sequence.
ACCESSION   DAETQN010000292 DAETQN010000000
VERSION     DAETQN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.299"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..689)
                     /gene="ipaH7.8"
                     /locus_tag="K0W68_004588"
     CDS             complement(<1..689)
                     /gene="ipaH7.8"
                     /locus_tag="K0W68_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HBI3075913.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEYYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRI"
     gene            1018..1197
                     /locus_tag="K0W68_004589"
                     /pseudo
     CDS             1018..1197
                     /locus_tag="K0W68_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1214..1912)
                     /locus_tag="K0W68_004590"
                     /pseudo
     CDS             complement(1214..1912)
                     /locus_tag="K0W68_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          614 a          384 c          466 g          616 t
ORIGIN      
        1 ttaatcctgt aacttgaatc taattgtgaa aaactctccg gaagagtggt tatctgatta
       61 aaatgaaaat aatattcctt ttgtctgata ttgtttctat cagaaaattg aggaaaagta
      121 accagattat tgcgctgagc acagagaatt tccagtttat ccggtaagtc gggtaatgtt
      181 gtcagcctgt tataataaac atgtaaaaat ttcaattttt cgggaagagc cggtaagtta
      241 tatagcctgt tattttcaac aaataatgat gttaaatggt ttggcaaaac aggaaggttt
      301 tccagttgat tagaacgaac acttaaagtt gttaatgact caggaagttc tggtatcaca
      361 gaaagcctgt tattatcagc attaagttct ttgagaaatg caggcagctt tggcaatgat
      421 gttaatttat tattttctac aatcaaagca gaaatatgct ttggtaattc gggaagagac
      481 gtcaaattta ggtctgataa atttaatact gcttcttgat tctgaaagca ctgggagagt
      541 ctgttaaaag caatgcctcg ctcttccccg ggagaagagt tcttttccca ttcagtcagg
      601 attctcagat aatattcatt actggttgag tttgagttaa tagaggggga gcaagaaact
      661 gatgagtgtg tattatttac agagaacata atgctaggtc aaccttatat ttgtgagaat
      721 aaatatttaa aattgtatat ttatacaaat ttcacttacc atttccgata ttaagtttta
      781 atagtttcca ttttacacat aacagaagtt agttgtaatt tatttattga ggttttctat
      841 ggaattcttt ttttattgtg actgcagtca ataataacac tgaattcaac attccagtga
      901 gcaaggaaat gtaatccgag taagtatatt tgttttacat catattttag ccaagctcat
      961 ttattcctat tatagaatga taaattaaaa ttactgtaaa aaaacgatac acatttaata
     1021 cgagggaggc cggcagaaaa acggcttgcg gtaaggcagg agaaatgtgt gccgcgaatg
     1081 aagtccttgt atgactggat aaagctccag aggaaaactt tgtcaaacca tgctgaaatg
     1141 gtaaaggcgt tcaactatat cctgaatcac tggaatgcac tgaatgagtt ttgccgggta
     1201 gtacttccaa tcattagaat atgtgctttt caatgaagga accgatgact ttttcgtgga
     1261 tttccacgga acgcgagaag cagattgttt tacgtgccag ccgcttgatg cgggggcgga
     1321 gggtcaggtt gttacgttca tacgttgcgt gaagattttg ccggtcaggt gtttctcctc
     1381 cggcacttct ctggcgtagc tgccccagtc atcgctggtc accatgccac catgccgata
     1441 cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg tggcccgcaa
     1501 gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca gtgttgttgc
     1561 gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc aacatcagcg
     1621 tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc cgtatgacgg
     1681 tattgatgcc gattttcagt gttctggcgg tataacagcc ccctgcgccg ttatgtgcca
     1741 tttcaacgat ctgctcctta acgccgggtt ttctggcttc ataagagtaa gtgagctgga
     1801 aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta cggccatgtc
     1861 gataaacttc atcggaatga cagcgcggac aatgaacagt aacggtagtc atggaaaacc
     1921 tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct tttgccgcaa
     1981 taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa aaaaattatc
     2041 ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata
//
LOCUS       DAETQN010000293         2060 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.300, whole genome shotgun sequence.
ACCESSION   DAETQN010000293 DAETQN010000000
VERSION     DAETQN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.300"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(135..1589)
                     /gene="ospC2"
                     /locus_tag="K0W68_004591"
     CDS             complement(135..1589)
                     /gene="ospC2"
                     /locus_tag="K0W68_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HBI3075914.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKMIDFLLKNGAVSGKRFGR"
BASE COUNT          649 a          363 c          329 g          719 t
ORIGIN      
        1 tctccaggat tcccggggcg ttcaccacat gcaatgtgtt gttgcagggg aacggtctgc
       61 accatcccct atatcgatca atataacaaa aatgacaacg tcagattcag ggcacaacct
      121 aaatatattt taggtcacct cccaaatcgt ttgcctgata ccgctccatt ttttaataaa
      181 aaatcaatca tttttgaatc tttgcttttc attgcattgt caagcatagt gtccccacta
      241 tttgcttttt gaaatctttt gtttacatca accaatccat tattaataaa atattctagc
      301 actttatagt tggcactatc ttcactaagt aaatactcaa catcatacag ctcacaatac
      361 atattgttgt tcattttctc catctctgcg acatcttgtt ttgtaaaatt aaatttagat
      421 agtaaataaa gtgccatatc agattttgca ttacctagag aataagcaag agcttgatga
      481 gccatttttt tatcagtcac cttattgtta atttcttcat aattggatgc tttaatggca
      541 tcctccaaag atatatctct gagtcttatc tttttgaagt tatctgtgct tagcattctt
      601 ggtatatgat actcaagctg aaacacaaag tttataattc tatccagact aacaggatca
      661 atgtttttat tataacaaaa ctctcggaat ctttgatctt tactctttct tataaaatca
      721 ataagatgca acccaattcc aagcctcata tctttagtat taaatattgg cacatcagtt
      781 tttccctgat taccatgcac atacctgcta acttcatcca caacctcttt aaagttatct
      841 aagaaaaatg attggtgctc atggtaaaaa acaggtggaa tagcattatc aaagtggtcg
      901 gttaatgtca tatatccata tggagagtca tgatcaatga tatacgcatt agcaccaaaa
      961 tctacagtat gatgtgtttt atttagtggg agtgtttctt gatgattgga aatttcaacc
     1021 ccaaaaaaaa caaaatcgtg gttagctatt tcttcaatat cctcagagaa tgtgttttta
     1081 tatgtttgta tattctcaga cttaatttta tcaaaggact taatattaag agtattattt
     1141 gacaggttag cgttactttg atgtctgaag ttaaaatcag aattaataat aatagagagc
     1201 aagtgacgtt catccgaaga aagaggatga gttttttgta ttatttcaga aagatagtgt
     1261 ctggacacct tatcgaggtg ctcaagtgac cgaaatgatg accgtgctcc agatggcgct
     1321 tcaatagaaa gatttaatga tttgaaactg gtcccttgag agaacatttt actataactt
     1381 tgcgctgcta tagacttcct taaaaagctt ttcacagtat cagcacagtg atctaaatgt
     1441 ttttcactta tgccagaaga ggatgagttt gttacgcatg atatgttttt ttgtaatgct
     1501 tttgtatctt tgttcggatt tatttttcca tcaacaagat caatattgtt ctgaacatta
     1561 atatgattta ctgcttcagg tattttcata aaacatcccc cattatgtgc gtttttatct
     1621 gcagttattt agaataatat tatctaactt aatattttct tgtatgtatt ataacgaaca
     1681 tattttaata tgttgtacga ttttcactct gaaatcccct gagtttaatc acacttcctt
     1741 tactgaaagg aattattgca acagtattat ttgtttttgg atatatatct tgcgccttgt
     1801 tatgtagtta atgctcagaa tatgatagat aaaatagaga cgcagaacag ggcatgctca
     1861 taacatgatg ataacataaa aaattagtaa caataaatga aagaatcatg ggcgctcaac
     1921 aaaaagagag ctaaatagct gcgcggaata gtaggctgtg tcccttaatc atctgagtta
     1981 tagtaactgt ctgtttacac aacatattga actatggctt gctacgacct tcccgatgag
     2041 gcatggacca tcatcaagcc
//
LOCUS       DAETQN010000294         2047 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.301, whole genome shotgun sequence.
ACCESSION   DAETQN010000294 DAETQN010000000
VERSION     DAETQN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.301"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(50..193)
                     /locus_tag="K0W68_004592"
                     /pseudo
     CDS             complement(50..193)
                     /locus_tag="K0W68_004592"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(197..916)
                     /locus_tag="K0W68_004593"
     CDS             complement(197..916)
                     /locus_tag="K0W68_004593"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3075915.1"
                     /translation="MTIGTKEKVIIINELRQCHPLSQLLVIADLPRSTFYYHVKRLNA
                     PDPYQLVKQVILRIYHQHKGRYGYRRIRLACRNESILLNGKTIRKLMKELGISSLIRR
                     KKYRTYRGEQGRTCNNLLKRQFYADRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEI
                     VSYNLTERPLASMVKSMLLDAVEQLNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQS
                     MSRRGNCLDNAAMESFFSTLK"
     gene            1057..1332
                     /locus_tag="K0W68_004594"
     CDS             1057..1332
                     /locus_tag="K0W68_004594"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3075916.1"
                     /translation="MVLESQGEYDSQWAVICSITPKIGCTPETLRVWVRQHERDTRGG
                     DGGLTTAERQRLKELERENRELRCSNDILRQASAYFAKAEFDRLWKK"
     misc_feature    1287..1403
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            1332..1481
                     /locus_tag="K0W68_004595"
     CDS             1332..1481
                     /locus_tag="K0W68_004595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075917.1"
                     /translation="MPLLDKLRGQYGVGPVCSELHIAPSTYYHCQQQDIILINAVPVR
                     STMTG"
     gene            1418..1816
                     /locus_tag="K0W68_004596"
     CDS             1418..1816
                     /locus_tag="K0W68_004596"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3075918.1"
                     /translation="MSATGHHPDKRSARAQHDDWLKREIQRVYDENHQVYGVRKVWRQ
                     LLREGIRVARCTVARLMAVMGLVGVLRGKKVRTTVSRKTVAAGDRVNRQFVAERPDQL
                     WVADFTYVSTWQGFVYVAFIIDVFAGYIVW"
     gene            1832..2038
                     /locus_tag="K0W68_004597"
     CDS             1832..2038
                     /locus_tag="K0W68_004597"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBI3075919.1"
                     /translation="METTFVLDALEQALWARRPSGTIHYSDKGSQYVSLAYKARLKEA
                     KLLASTGSTGDSYDNAMAEIIKGL"
BASE COUNT          518 a          457 c          499 g          573 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 taaggttata tgagactatt tctccattat acaggtccat gattggtgat aaatagagct
      481 tcctgccatc gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag
      541 cataaaattg tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac
      601 ggtacttctt gcgcctgata agtgagctga tacccagttc cttcattaat tttctgatgg
      661 ttttaccatt aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat
      721 aacgcccttt atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat
      781 ctggcgcatt aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa
      841 ccagtaattg tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt
      901 ttgttccaat cgtaattttt cctgagctaa ggctttcagc ttttttagat aatgaaccgc
      961 cccgggaatc ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac
     1021 tcgttttccc ccgaagtccg tcaacgggca gtccgtatgg ttctggaaag tcagggcgaa
     1081 tatgactcac aatgggcggt aatttgttcc attactccaa agattggctg tacaccggag
     1141 actctgcgtg tctgggttcg ccagcatgag cgggatacca ggggcggtga tggagggctc
     1201 accaccgctg aacgtcagcg tctgaaagag ctggagcgtg agaatcgtga actgcgctgc
     1261 agtaacgata tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc
     1321 tggaaaaaat aatgccactg ctggataagc tgcgtgggca gtacggggtc ggaccggtat
     1381 gcagtgaact gcatattgcc ccgtcaacgt attaccactg tcagcaacag gacatcatcc
     1441 tgataaacgc agtgcccgtg cgcagcacga tgactggctg aagagagaga tacagcgcgt
     1501 atacgatgaa aatcatcagg tgtacggtgt gcgtaaagtc tggcgccagt tgttacggga
     1561 aggaatcagg gtggccagat gtacagtggc gcgcctcatg gcggttatgg gacttgtcgg
     1621 tgttctccgg ggtaaaaagg tccgcactac cgtcagccgg aaaaccgttg ccgcaggtga
     1681 ccgcgtaaac cgtcagttcg tggcagaacg tcctgaccag ctgtgggtgg ctgattttac
     1741 ttacgtcagc acatggcagg gcttcgtcta tgtggcgttc atcattgatg tgtttgccgg
     1801 atacatcgtg tggtgacgag tctcatcgtc tatggaaacg acattcgtgc tggatgcact
     1861 ggagcaggcg ttgtgggccc gtcgtccgtc tggcaccatc cattacagcg ataaaggctc
     1921 tcagtatgtg tcactggcct ataaggcgcg actaaaagaa gcaaaactgc tggcatcaac
     1981 agggagtaca ggcgactcgt atgacaacgc gatggcagag atcatcaagg gcctttaggt
     2041 aatgact
//
LOCUS       DAETQN010000295         2038 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.302, whole genome shotgun sequence.
ACCESSION   DAETQN010000295 DAETQN010000000
VERSION     DAETQN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.302"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="K0W68_004598"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="K0W68_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HBI3075920.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(1452..1883)
                     /locus_tag="K0W68_004599"
                     /pseudo
     CDS             complement(1452..1883)
                     /locus_tag="K0W68_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          538 a          441 c          494 g          565 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tatgtgacag
      901 taatacgctg cttcatgata agccctcaaa aacatactta tgatatgtat atgagtatat
      961 attaatggta caaaccatca atatccataa aaaatacacc attaattcgc tataagtttt
     1021 taatacaatt ttttctgctg cgattttttt atacgataat tagcatactt ataacaccgt
     1081 aagtaaatta catgaaagca ggcacagcgt gatccgattt ttctgcgtat aacgattttc
     1141 cggaagtgta atacggttgc gccccgggcc gggccccctt ttctgttgtt gtgtacgggc
     1201 ccacagagaa tggtaccgga ggcgggctca gaagtactga gtcgggtgat cgcgtcaggc
     1261 cgcgaagtac tgacacgggt attggtgact ggtgatgaag tactgccccg ggtatgattt
     1321 caggctgatt caggttctga ttctctgtca gtccgcgtgg ttgctggcct ggctgatttc
     1381 ctgttgtcag gcgttcaaca accgactgct ggttatagcc cggttcacga acacctgacg
     1441 gcaggtatca ctcacccggt gaaggtaaat ccctcaagag gcagcaactc tgccagtcgc
     1501 tcctccggcc attccggcag acgcgtcagg acgtccgtca gccaggcatg tggctccaga
     1561 ccattacgtt ttgctgttta cagaaggctc attatctgtg ccgcccggtt tccggccatc
     1621 agtgaaccgg cgaacagcca cgctttccgt cccataacaa ccgggcgaat ggcacgctcg
     1681 cagatattgt tgtccagggg caccgctccg tcacccagga agcagctcag ttcatcgcgc
     1741 cggttcagaa tatagttaat cgccttattt aatggcccgt cgggcggaca gcacggctcc
     1801 tgctcttcaa gccaggcaaa caggtcattc actatcggcc gggagtaccg ttatcgccac
     1861 tgccgtattt tttccacagg gcgctgtcag cgccagtgat ataagacggt aattcgccat
     1921 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttctcacacc agaaaggaca
     1981 tcaacatgct gagcagagag gacttttaca tgataaagca aatgcgccag cagggcgc
//
LOCUS       DAETQN010000296         2005 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.303, whole genome shotgun sequence.
ACCESSION   DAETQN010000296 DAETQN010000000
VERSION     DAETQN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.303"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..389
                     /locus_tag="K0W68_004600"
     CDS             <1..389
                     /locus_tag="K0W68_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075921.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="K0W68_004601"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="K0W68_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBI3075922.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="K0W68_004602"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="K0W68_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBI3075923.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="K0W68_004603"
     tRNA            complement(1806..1881)
                     /locus_tag="K0W68_004603"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="K0W68_004604"
     tRNA            complement(1890..1966)
                     /locus_tag="K0W68_004604"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAETQN010000297         1998 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.304, whole genome shotgun sequence.
ACCESSION   DAETQN010000297 DAETQN010000000
VERSION     DAETQN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.304"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..691
                     /locus_tag="K0W68_004605"
     CDS             <1..691
                     /locus_tag="K0W68_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBI3075924.1"
                     /translation="WSQKDVFMTYRTTGLATYRNYDFFGLIEGLNFAAQYQGKNERTD
                     NSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANPLNASGKNA
                     ELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQFDFGLRPSLA
                     YLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFTRAVDIRTD
                     NIVATGITYQF"
     gene            855..1337
                     /locus_tag="K0W68_004606"
                     /pseudo
     CDS             855..1337
                     /locus_tag="K0W68_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1452..>1998
                     /locus_tag="K0W68_004607"
     CDS             1452..>1998
                     /locus_tag="K0W68_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBI3075925.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT          668 a          413 c          344 g          573 t
ORIGIN      
        1 ctggagtcag aaagatgtct tcatgacata ccgtaccacc ggtctagcaa cctatcgcaa
       61 ctacgatttc tttggcttaa ttgaagggct gaactttgcc gcgcaatatc aaggcaaaaa
      121 tgaacgtact gacaacagtc atctttatgg tgctgactac acgcgtgcca acggtgacgg
      181 tttcggtatc tcctcaactt atgtttatga tggctttggt atcggtgcgg tatataccaa
      241 atccgatcgg acaaatgcgc aggaaagagc cgctgctaat cctctcaatg cctccggtaa
      301 gaatgcagaa ctatgggcca caggtataaa gtatgatgcc aacaacatct actttgcagc
      361 taattacgct gaaacattaa acatgaccac ctatggggat ggttatatct ctaacaaagc
      421 acaaagtttt gaagtggtgg cgcaatatca attcgacttc ggcttgcgcc catcactcgc
      481 ttacctgaaa tcgaaaggca gagatctggg ccgctacggc gaccaggaca tgattgagta
      541 tatcgacgtt ggtgcgacgt atttcttcaa caaaaatatg tcgacctatg ttgattataa
      601 aatcaacctg attgatgaaa gcgactttac ccgtgccgta gatattcgga ccgataacat
      661 cgtcgctacg ggcattacct atcagttcta acgtcctctc ctgaccagcc cttctctggg
      721 ctggtttttg atttaagtgc tgctgtatga ctccttaagt aaaaatgagc aatttttgat
      781 atttctggtc attcacctca attaatatta ttccatatcc tccggggaca tcttactata
      841 atatttattg atgagtgatt atgaccaata ttaatacagc ttgtgtaaaa aataatgcca
      901 gttatcaact taataacgca ttacctaaca aagaaaccat ctccagcaat ttttgtgaac
      961 gactagaaca atggggtaat aaatcggtta ataatggtga agaaagagca attgccgtgg
     1021 agcgaattaa agaagcttac aattcgaaca tggcatctct tgatttgtca tatcttgatt
     1081 tgagtgaatt accgcctatc ccctcgacag ttaatacgtt aaatctggaa aacaattgtc
     1141 taacttgtct tgacttagct gacaatgcca gtctcgttaa tatcaacctc agcgttaata
     1201 aaattaacac gataaccttt ccaaaagaat caaaactgga aaacatttat attgatcata
     1261 acaatctgga aagtttagat ttaaaaaatc agcattcatt ggttaatctg gaagcgcaaa
     1321 ataacaaact gacaaaaatt aatatttctg atagttataa actgaaattt cttaatcttg
     1381 attataacaa gctagcatcg ctggatgtct cccggcaaga atctctgatt gagttaagtg
     1441 cccatcacaa tatgatcact gaccttatat tacacaatca tcccaggatg aaaacaatca
     1501 ctttaaacga caaccatatt gcacatttaa acgccaaaaa cactacaaaa ctggaatatt
     1561 taaacttaag caataacaat ttactgccaa ccaatgacat tgatcaacta atatcatcaa
     1621 aacatctttg gcatgtatta gttaacggca tcaacaatga tccacttgca caaatgcagt
     1681 actggactgc agtaagaaat ataattgatg acactaatga agtgaccatt gatttatcag
     1741 gacttaattt aaccactcaa ccaccagggc tgcaaaactt cacatctatc aatcttgata
     1801 ataaccaact cacacatttt gatgcaacca actacgatag actcgtaaag ctaagtctga
     1861 atagtaatgc tcttgagtca ataaattttc ctcaaggcag aaatgtaagt attacacata
     1921 tatctatgaa taataatgct ctcagaaata ttgatataga taggctttca tcagttactt
     1981 attttagtgc ggcacata
//
LOCUS       DAETQN010000298         1995 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.305, whole genome shotgun sequence.
ACCESSION   DAETQN010000298 DAETQN010000000
VERSION     DAETQN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.305"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            270..539
                     /locus_tag="K0W68_004608"
     CDS             270..539
                     /locus_tag="K0W68_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075926.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            536..762
                     /locus_tag="K0W68_004609"
                     /pseudo
     CDS             536..762
                     /locus_tag="K0W68_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            791..1381
                     /gene="ospG"
                     /locus_tag="K0W68_004610"
     CDS             791..1381
                     /gene="ospG"
                     /locus_tag="K0W68_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HBI3075927.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1720..1995
                     /locus_tag="K0W68_004611"
                     /pseudo
     CDS             1720..1995
                     /locus_tag="K0W68_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          563 a          439 c          471 g          522 t
ORIGIN      
        1 tgactgacga ggtactggcc ctgcgattgt ctgaaaacgg ctcacaactg caccattcat
       61 aatcacatcg cataaaactg tcagcgccag tgataggaca ggtgaaacct gtcaatacgt
      121 accggcttac gccgggcttc ggcggtttta ctcctctgcc atatgcaaca acggagtgcc
      181 gccttacatg ccgctggcgc ggcatatcct ggtgacgata tctgaacagt tatatactgt
      241 gtatatacgt ggtaatgacg aggtggcaaa tggcacaggt taatatgagt gtaagaatcg
      301 acgcagaact gaaggatgct tttatggccg ctgcaaaaag catggaccgt aatggctctc
      361 agttaatccg ggattttatg cgccagaccg ttgaacggca gcacaattct tggttccgtg
      421 accaggttgc ggcaggccgt cagcaactcg aatgcggtga tgtgcttccc catgacatgg
      481 tcgaatcctc tgctgctgca tggcgggatg aaatgagcag gaagattgct gataaatgat
      541 ggagatgttc tggaccatgc tggccatcca ggacaggaag cgcattcgta gtacattgcg
      601 gagcagaacc tgatggccgc cattgaaccg gacgaacgga ttggttattc agccagcagc
      661 ctggcagggc agccgtataa aggccgcaac ggtcgtgtgg aaggaacatc tggaccacac
      721 aaggtagcgt gtaacgttat tttgtgtgaa aatctcttgt gatttttaaa tatcgtaaca
      781 aaggtatgac atgaaaataa catctaccat tattcaaaca ccttttccat ttgagaataa
      841 taattctcat gctggcatag taacggagcc cattctcggt aagttaatag gtcaggggtc
      901 gacagcagaa atctttgaag atgtgaatga ttcatctgct ttgtataaaa agtatgatct
      961 tattggcaac cagtacaatg agattctgga aatggcttgg caagaatctg agctttttaa
     1021 tgctttttat ggcgatgaag catccgttgt tatacagtat ggcggagatg tgtacctccg
     1081 aatgctgcgc gtgcctggga ctccccttag tgacattgat acagctgata tccctgataa
     1141 tatagagagc ctttatctac agttgatatg taaattgaat gagttgagta taatccatta
     1201 cgatcttaat acaggtaata tgctgtatga taaagaaagt gaaagtttat tcccaataga
     1261 ttttcgcaat atttatgctg aatattacgc tgcaaccaaa aaagataaag agattatcga
     1321 ccgacgatta caaatgcgta caaatgattt ttattcgtta ttaaacagga aatatttata
     1381 gccgtatttg ttgatgctat aagccagagg ctcttctatg agaatatggg gaccattccc
     1441 tcgcgatatt aacgtaatat cttaattgta tgcaccgcca gaatgcgttc agtgcgatca
     1501 cggcagcaga caggtaaaaa tagcaacaaa ccacccgaaa aaccgccgcg atcgcgtatg
     1561 ataaatttta accacatgca tacctgtgca gcaaggcgaa tcacgcacgg atttccagcc
     1621 agagacacct gaaacgtaac cggctcattt aaaccgtctg gtttgttacc tccggttcca
     1681 caaaaataat gtccatcatt ttgaagtgga cactatcgta tggaataccg gacctgaatc
     1741 actgaagctt tacgccttca tttcgaagaa catttaccac gggttgtggc cggacgtcgc
     1801 ctgggcgtac caaaatcaac tgtctgtggt atgttcgtgc gcttccgcaa cgctggcctt
     1861 tcatggcctc tgcccgcagg tatgtcggag caggaacttg atgcccttct ttacggaagt
     1921 gcctccacag tacctgtcgt acttactgaa agcaccgtta tgcccaaact cccggtggta
     1981 aaaaaacgtc cccgg
//
LOCUS       DAETQN010000299         1937 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.306, whole genome shotgun sequence.
ACCESSION   DAETQN010000299 DAETQN010000000
VERSION     DAETQN010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.306"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1249
                     /locus_tag="K0W68_004612"
     CDS             <1..1249
                     /locus_tag="K0W68_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBI3075928.1"
                     /translation="YRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVL
                     DSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMA
                     SPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLD
                     EMIVLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYI
                     ENRMYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVD
                     FDSDKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFQPLQRDMIGKFGAAKDQ
                     WFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWK
                     KSGPEMASLEAWLEEHEKYRSVA"
     gene            1246..1632
                     /gene="trbI"
                     /locus_tag="K0W68_004613"
     CDS             1246..1632
                     /gene="trbI"
                     /locus_tag="K0W68_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HBI3075929.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            1629..>1937
                     /locus_tag="K0W68_004614"
     CDS             1629..>1937
                     /locus_tag="K0W68_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HBI3075930.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     R"
BASE COUNT          447 a          503 c          588 g          399 t
ORIGIN      
        1 ctaccgtaac cagctggcgt ttatcgatat tttcttccgg ggaatgaata acaccaacta
       61 caacatggcg gtctgtggca cctccggggc cggtaaaacc gggctgatac agccacttat
      121 ccgcagcgtg ctggactccg ggggctttgc cgtggtgttc gacatggggg atggctacaa
      181 gtccctgtgt gagaacatgg gtggcgtata tctggacggt gaaaccctgc gttttaaccc
      241 gtttgcgaac atcaccgata tcgaccagtc agcggagcgt gtccgtgacc agttgtcagt
      301 gatggccagc cccaacggta acctggatga agtgcatgaa ggtctgctgc tgcaggcggt
      361 cagggcctcc tggctggcca aagagaacag ggcacgtatt gatgacgtgg tggatttcct
      421 gaaaaacgcc agtgacagcg agcagtatgc cgggtcaccg actatccgca gccgtctgga
      481 cgaaatgatt gtgctgcttg accagtacac tgccaacggc acttacggcc agtattttaa
      541 ctctgatgag ccgtccctgc gggatgacgc aaaaatggtg gtgctggagc tgggcggact
      601 ggaagaccgt ccgtcactgc tggttgcggt gatgttctcc ctgattatct acatcgagaa
      661 caggatgtac cgcacgccgc gtaacctcaa gaaactgaac gtcattgatg aaggctggcg
      721 tctgctggac ttcaaaaatc acaaggtcgg tgagtttatt gagaaaggtt accgtaccgc
      781 ccgtcgtcat accggtgctt atatcaccat cacacagaac atcgtcgact ttgactctga
      841 caaggcctcc agtgcggccc gtgcggcatg gggtaactcc tcctacaaaa ttatcctcaa
      901 acagagtgcg aaggagttcg caaaatacaa ccagctgtat ccggaccagt tccagccact
      961 gcagcgcgac atgattggta agtttggtgc ggccaaagac cagtggttca gttccttcct
     1021 gctgcaggtg gaaaaccatt cctcctggca ccgcctgttt gtggacccgt taagccgcgc
     1081 catgtacagc tctgacggcc cggattttga gtttgtgcag cagaaacgta aagaggggct
     1141 gagtattcat gaggcggtgt ggcagctggc gtggaagaag tcagggccgg agatggcttc
     1201 gctggaagcc tggctggaag aacatgagaa atacaggagt gttgcatgac cacaacgcaa
     1261 aaaacgactg atgttacagc cccccgacgc agccactggt ggtggacggt gccgggatgc
     1321 ctggcaatgg tgttactgaa cgcagccgtc agttacggca ttgtcagact gaacgcaccg
     1381 atgactgccg ccttcaacat gaaacagact gtggatgcat tttttgacag cgccagtcag
     1441 aaacaactgt cagaagcaca gtcaaaagcc ctttcagcgc gttttaacac ggcactggaa
     1501 gccagcctgc aggcgtggca gcagaaacac cacgcggtca ttctggtgtc gcctgccgtg
     1561 gtacaggggg ctccggatat cacccgggaa atccagcagg atattgcccg gcgaatgaga
     1621 gcggaaccat gagatgccgg gggctgattg ccctgctgat atgggggcag agtgtggccg
     1681 ccgccgatct tggcacctgg ggtgatctgt ggccggtaaa ggagccggac atgctgacgg
     1741 tgatcatgca gcgcctgacg gcactggagc agtccggtga gatgggccgg aaaatggatg
     1801 cctttaagga gcgggtgatc cgcaacagtc tgcggccccc tgccgtgccc ggtatcggac
     1861 gcacggagaa atacagcagc cggttgtttg acccgtccgt cagactggct gcggatatcc
     1921 gggataacga agggcgg
//
LOCUS       DAETQN010000300         1937 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.307, whole genome shotgun sequence.
ACCESSION   DAETQN010000300 DAETQN010000000
VERSION     DAETQN010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.307"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            73..1461
                     /locus_tag="K0W68_004615"
     CDS             73..1461
                     /locus_tag="K0W68_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBI3075931.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQK
                     LVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDE
                     FCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKASDER
                     LYVNKQNKNSRS"
     gene            1504..>1937
                     /gene="yeaK"
                     /locus_tag="K0W68_004616"
     CDS             1504..>1937
                     /gene="yeaK"
                     /locus_tag="K0W68_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HBI3075932.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSV"
BASE COUNT          509 a          451 c          456 g          521 t
ORIGIN      
        1 tttatcgccg caagtgtcat tgtgctgaca tcttccttcc ttatttttga acttgtcgcc
       61 agcgacagag caatgagtgc ctatctgcgc tatatcgtgc agaaagcaga ctcctccttt
      121 ctttatgata agtatcagaa tcagagtatt gccgcgcatg tgatgcgcgc tctcgctgct
      181 gagcagtcgg aagtgtcgcc agaacagcgg cgcgccatct gcgaggcttt tgagtctgcc
      241 aataataccc atggcttaaa cctgactgcc cataaatacc cgggcttacg cggcacacta
      301 caaaccgcat ccactgactg cgacacaatt gtggaagctg cagcactatt acccgctttt
      361 gatcaggcag tggaaggcaa ccgccaccag gatgattacg gttcaggtct tgggatggcc
      421 gaagagaaat ttcactatta tctcgatctc aatgaccgct atgtctattt ttatgagccg
      481 gttaatgttg aatactttgc gatgaataac tggtccttcc tgcagtcagg aagtattggc
      541 atcgatcgca aagatattga aaaggtattt accgggcgta ccgtattgtc gagcatttac
      601 caggatcagc gtactaaaca gaacgtgatg agtttgctga cgccggtata tgtcgcaggg
      661 cagctaaaag ggattgtgct gctggatatt aacaaaaaca atttgcggaa tatcttttac
      721 actcatgacc gccctctcct ctggcgtttt ctcaatgtca cgctaaccga taccgattcg
      781 gggcgcgaca ttatcatcaa ccagagcgaa gataatctgt tccagtatgt cagttacgtc
      841 catgacttac cgggcggcat tcgtgtctcg ttatccattg atattcttta ctttatcacg
      901 tcttcgtgga aaagcgttct gttctggatt ttgacggcgt taattttgct gaatatggtc
      961 cgaatgcact tccgtttata ccaaaatgtg tcgcgagaaa atattagtga tgcgatgact
     1021 ggactgtata atcgcaaaat tttaacccct gaactggagc agcggttgca gaaactggtg
     1081 caatccggtt cttcggtgat gtttattgca attgacatgg acaagttaaa gcaaataaat
     1141 gacaccctcg gtcatcagga gggggattta gcgattacgt tattagctca ggcgattaaa
     1201 caatcgattc gtaaaagtga ttatgccatc cgactcggtg gcgatgaatt ctgcatcatt
     1261 cttgtcgatt cgacgccgca aattgcagca caactgcctg aacgtatcga aaaacgtctg
     1321 caacatatcg cgccgcagaa agaaatcggc ttctcttccg gtatttacgc gatgaaagaa
     1381 aacgatacgt tacatgatgc gtataaagct tccgatgagc gtttatatgt caataagcag
     1441 aacaaaaaca gccgttcatg ataaccttct gtggttgttt gcttgtaatc tcaggagcgt
     1501 gaaatgactg aaatggctaa aggaagcgtg acgcatcagc ggttaatcgc tttattatca
     1561 caagaaggtg ctaacttccg cgtcgttacc catgaagcgg taggtaaatg tgaagcggtg
     1621 tcagaaattc gtggcaccgc tttaggccag ggagctaagg cactggtatg taaagtcaaa
     1681 ggcaatggcg taaatcaaca tgttctggcg atcctcgctg ccgatcaaca ggccgatctg
     1741 agccaacttg ccagccatat aggcggatta cgcgcttcgt tagccagccc tgctgaagtc
     1801 gatgaactca caggctgtgt cttcggcgcg atcccccctt tcagcttcca tccaaaactc
     1861 aaactggttg ccgaccctct actcttcgaa cgatttgatg aaatcgcctt caatgcaggg
     1921 atgctggata aatccgt
//
LOCUS       DAETQN010000301         1929 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.308, whole genome shotgun sequence.
ACCESSION   DAETQN010000301 DAETQN010000000
VERSION     DAETQN010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.308"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..898
                     /locus_tag="K0W68_004617"
     CDS             <1..898
                     /locus_tag="K0W68_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HBI3075933.1"
                     /translation="NAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLR
                     QAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE
                     GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVSIAGEGRPWNPGA
                     ITDGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENK
                     PDLPDGKTELAVREIAGQERDRAAITEREAALPESVLRESQREREAVREVARENLLQE
                     RLQQMERDMVRDLQKEKTPGGD"
     gene            918..1665
                     /gene="traX"
                     /locus_tag="K0W68_004618"
                     /pseudo
     CDS             918..1665
                     /gene="traX"
                     /locus_tag="K0W68_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            1720..>1929
                     /locus_tag="K0W68_004619"
     CDS             1720..>1929
                     /locus_tag="K0W68_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBI3075934.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNL"
BASE COUNT          423 a          488 c          653 g          365 t
ORIGIN      
        1 caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg accgggaggt
       61 catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg cggcaggccg
      121 tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt ttattgctcc
      181 gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta acggcaagtc
      241 cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc ggggattcag
      301 cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc agggcagccg
      361 taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg cccgtgataa
      421 tcctgacagt ggtgtggtgg taagcattgc cggtgaaggc cgtccgtgga atcccggtgc
      481 catcaccgat ggccgcgtgt ggggggatat cccggacaac agcgtccagc cgggagccgg
      541 aaatggcgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag aggccatccg
      601 ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga acaaacctga
      661 cctgccggac ggcaaaacag agctggctgt cagggaaatt gccgggcagg agcgtgaccg
      721 ggctgccata actgaacggg aagccgcgct gccggagagt gtgctgcgtg aatcacaacg
      781 ggagcgggaa gcggtccggg aggttgcccg ggaaaatctg ctgcaggagc gtctgcagca
      841 gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg gagactgata
      901 caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac gattcactgg
      961 ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga cagcgggata
     1021 tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg atattccagc
     1081 tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt gccctggtgt
     1141 gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac cggctgtggg
     1201 gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccctgg tatgagggga
     1261 atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa acgcgcagtg
     1321 ggtggcgtac agcagcggcc attcttctga tggccctgtt gggggccttt gtccggcacc
     1381 agttacggca ttgccgggct gctgatgctg gcagtcagcc accgactgta ccgggcggaa
     1441 gacagagcgg aacgtctggc gctggtcgcc tgcctgctgg ctgttattcc ggcgcttaac
     1501 cttgccacca gtgatgcagc ggcggtggcc ggtcttgtga tgacggtgct gaccgttggt
     1561 ctggtgtcgt gtgcaggaaa atcattaccc cgtttctggc ccggggattt tttcccggta
     1621 ttctacgcct gtcatctggc tgtgctgggc gttctggcgc tgtgacgggt gtggtatctt
     1681 tgaccgtaac aggatgattc gtcagaggca gaacacagca tgacagagca gaagcgaccg
     1741 gtactgacac tgaagcggaa aacggaaggg gagacgcctg tccgcagccg gaaaaccatc
     1801 atcaatgtca ccacgccacc aaagtggaag gtgaaaaagc agaagctggc cgagaaagca
     1861 gcccgggaag cagagctggc ggcaaaaaaa gcgcaggcca gacaggcact gtccatttat
     1921 ctgaacctg
//
LOCUS       DAETQN010000302         1899 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.309, whole genome shotgun sequence.
ACCESSION   DAETQN010000302 DAETQN010000000
VERSION     DAETQN010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.309"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(89..643)
                     /locus_tag="K0W68_004620"
     CDS             complement(89..643)
                     /locus_tag="K0W68_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBI3075935.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(640..930)
                     /locus_tag="K0W68_004621"
     CDS             complement(640..930)
                     /locus_tag="K0W68_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBI3075936.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(930..1529)
                     /locus_tag="K0W68_004622"
     CDS             complement(930..1529)
                     /locus_tag="K0W68_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBI3075937.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1601..1813)
                     /locus_tag="K0W68_004623"
     CDS             complement(1601..1813)
                     /locus_tag="K0W68_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075938.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          472 a          501 c          387 g          539 t
ORIGIN      
        1 taaagcgggc tctttcaaat gtccatgtct gctattcgcc tcgcggtaca gctttgcgaa
       61 gcgtacaaga attgaagcag tttttacgtc aaaaagcaat aacttttttc tctataccaa
      121 aagccataac cattggtttg tacaaaataa attctgccac ctttagccaa tgctcaatgc
      181 gtctttcaca ggttcttaaa ctccatttcg ggtgtgcatc attcagcagt tcagccattt
      241 tgcgtttggt catcccccgc ccctcatacc gttgccgaag gacgctaatc aatcctggat
      301 gctctgccag cacctcactt atgacccgat caatacataa cgcctctgca tcagtacaat
      361 gcgccagcca gctcttttgc ttaccgttaa tcatatcccg caaaaaagcc tcaagttcag
      421 acttgttcag acctgctttt ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca
      481 gctttttaga ggtcaacatc aggttaaaca tattccccgt cttaccgcca ccaatatacg
      541 accagcgccc ccacatacgt agttttccct gaatccagac actttccagc gtggtgagac
      601 gaaggtgttc tccgcttttt cctgtattcg ttgggtaaat cataaatatc cctcctttct
      661 ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt cttctttggt
      721 gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg cacgagctac aggcaatcgc
      781 tgcctgcata tcgtgtggct ttatcgctgt tccgcacgtt cccgccagtc ggtaatgcgc
      841 cagcacagac gtttccggat cgtgattgca gtagccagga attctgacgg tgcacatctg
      901 cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagcagctg cgcggccaca
      961 ttttcgactt gttccggaga ggaaaattta cggaacagaa tccagttcca cagcacattc
     1021 agaacagatt tataaacctg ctgaaactcg gtttcgtcca tattcgcaaa agcgatggat
     1081 tttgcccgac gcccgcggct accgtccgga taaaaatgtt cggtgtaaaa tccagcctga
     1141 atggttaccc actcgcgaaa agcctcaaac gactttagca atgccgtatc ccgggttctg
     1201 cgtgtcgcaa ctgtattcag atattgctct gcggcttcgc tcagagctgg cgtatgttcc
     1261 cgacctactg attcgcacag gtaatcaacg aaaccagaca gcagttctcg ttcgcgaggc
     1321 gtgatcgccc caccgaccgg agtccagtaa tcgaatccca gttgcaggag tttgaaaaaa
     1381 cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca ctcacctgtt
     1441 ttgatttgat gcagaaaatc gcaactctcc ggcgtcgccg ggagaagtaa tccggaagag
     1501 gtttgtttga tcagttgtat atgcgccatc gtagttctcc gctggcgcag tagaatgggt
     1561 gttcagcccg ttatgtagta taccagaatt aatgccaata ctaacaggat gctctgactc
     1621 gcaattcatc cagcagttta tcatttccca taatgtcact taccctcatc ggtaaaaaaa
     1681 ttgcctttcg accattacga tacatcattg attttttggt ttcagggaag taatccattt
     1741 cgactataac tgacaggtca tcacgacgta tgactgcgta tttactacta aatagtttct
     1801 ttattttttc cacgatgcct ccaggtttat aagtacaaac ggttatatca acatagagac
     1861 aaaaggtgat gctgccaact tactgattta gtgtatgat
//
LOCUS       DAETQN010000303         1884 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.310, whole genome shotgun sequence.
ACCESSION   DAETQN010000303 DAETQN010000000
VERSION     DAETQN010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.310"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            76..345
                     /locus_tag="K0W68_004624"
                     /pseudo
     CDS             76..345
                     /locus_tag="K0W68_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            460..630
                     /locus_tag="K0W68_004625"
     CDS             460..630
                     /locus_tag="K0W68_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075939.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(757..1314)
                     /locus_tag="K0W68_004626"
     CDS             complement(757..1314)
                     /locus_tag="K0W68_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBI3075940.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1311..1586)
                     /locus_tag="K0W68_004627"
     CDS             complement(1311..1586)
                     /locus_tag="K0W68_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HBI3075941.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1771..1884
                     /locus_tag="K0W68_004628"
                     /pseudo
     CDS             1771..1884
                     /locus_tag="K0W68_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
BASE COUNT          528 a          387 c          457 g          512 t
ORIGIN      
        1 ttttcattga tttcaccatg ttatagtttt atttgtgaat taaatcaatt atggcgatga
       61 attacaaggg gttaaatgct gccgcagcat agcgatattg aaatagccta gtatgcttcg
      121 atacagcagg agccgaatgg ctggaagacc gtcaccacac agttctacat ccaggaattt
      181 agtgagtata ttgcgccact gcaggatgct gtagatctgg aaatcgcaac ggaggaagaa
      241 agatcgttgc tggaggcatg gaataaatat cgggtattgt tgaatcgtgt tgatacatca
      301 actgcacctg atattgagtg gcctgcaaat cctgtcaggg agtaatcatt gggattatgc
      361 cgcagacacg tcgtatgcag gaacgtgctg cggttagttt gtgagctttc gatagtggtt
      421 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      481 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      541 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      601 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      661 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      721 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      781 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      841 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      901 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      961 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
     1021 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
     1081 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
     1141 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
     1201 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
     1261 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
     1321 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1381 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1441 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1501 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1561 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata
     1621 aaattaaagc gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat
     1681 caggcagtta aaaacacgga aaacggtata aaaaagcgcc tcaaaatcct gttttttctt
     1741 tcagatacaa tgtcttattg cattttttaa aacatgtcag tgtggtacat ggatatcgat
     1801 accatcgcca attataaact gggcggtgca cagcaacatg atagcgtacg cctcgatccg
     1861 tgggtgttta tgttctcagc agga
//
LOCUS       DAETQN010000304         1838 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.311, whole genome shotgun sequence.
ACCESSION   DAETQN010000304 DAETQN010000000
VERSION     DAETQN010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.311"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1087
                     /locus_tag="K0W68_004629"
     CDS             join(<1..183,183..1087)
                     /locus_tag="K0W68_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3075942.1"
                     /translation="ERIGVAAAARELSLYESQLYAWRSKLQQQMTSSERESELAAENA
                     RLKRQLAEQAEELAILPKGRDILCEAPEMKYVFIEKHQAEFSIKAMCRVLRVARSGWY
                     TWCQRRTGISPRQQFRQHCDSVVLAAAFTRSKQRYGAPRLTDELRAQGYHFNVKTVAA
                     SLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGW
                     LYLAVVIDLWSRAVIGWSMSPRLTAQLACDALQMALWRRKRPRNVIVHSDRGSQYCSA
                     DYQALLKWHNLRGSMSAKGCCYDNACVESFFHSLKVECLHGEHFISREIMRATVFNYI
                     ECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            1427..1838
                     /locus_tag="K0W68_004630"
                     /pseudo
     CDS             1427..1838
                     /locus_tag="K0W68_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          453 a          460 c          488 g          437 t
ORIGIN      
        1 ctgaacgcat cggtgttgct gccgcagccc gtgaactcag cctgtatgaa tctcagctct
       61 atgcgtggcg cagcaaactg cagcaacaga tgacctcgtc tgagcgtgaa agtgaactgg
      121 ccgctgaaaa tgcccgcctg aagcggcaac tggcggagca ggcagaggaa ctggctatcc
      181 tccaaaaggc cgcgacatac tttgcgaagc gcctgaaatg aagtatgtct ttattgaaaa
      241 acatcaggct gagttcagca tcaaggcaat gtgccgcgtg ctccgggtgg cccgcagcgg
      301 ctggtatacg tggtgtcagc ggcggacagg gataagccct cgtcagcagt tccgtcagca
      361 ctgcgacagc gttgtcctcg cggcggcctt tacccggtca aaacagcgtt acggtgcacc
      421 acgcctgacg gatgaactgc gtgctcaggg ttaccacttt aacgtaaaaa ccgtggcggc
      481 aagcctgcgc cgtcaggggc tgagggcaaa ggcctcccgg aagttcagcc cggtcagtta
      541 ccgcgcacat ggcctgcctg tgtcagaaaa tctgctggag caggattttt acgccagtgg
      601 tccgaaccag aagtgggcgg gagacatcac gtacttacgt acagatgaag gctggctgta
      661 tctggctgtg gtcattgacc tgtggtcacg tgccgttatt ggctggtcca tgtcaccacg
      721 cctgacggcg caactggcct gcgatgccct gcagatggcg ctgtggcggc gtaagagacc
      781 ccggaacgtt atcgttcact cggaccgagg aagtcagtac tgttcagcag attatcaggc
      841 gctgctgaag tggcataatc tgcgtggcag tatgagcgca aagggctgct gctacgataa
      901 tgcctgcgtg gaaagcttct ttcattcgct gaaagtggaa tgtctccacg gagaacactt
      961 tatcagccgg gaaataatgc gggcaacagt gtttaattat atcgaatgtg attacaatcg
     1021 ctggcgacgg cacagttggt gtggcggcct cagtccggaa caatttgaaa accagaacct
     1081 cgcttaggcc tgtgtccata ttacgtgggt aggatcactt acgccctgtg gttgaaacgt
     1141 gagcatattg ttttcagatg ctgttgtctt gcttcagagt gatctgtcct ccggatttac
     1201 gcaaagttat ccacaggcac tgtgcattta aaaaacttat cttttcttct tgattttaaa
     1261 aactcagatc gtcatatcat tatgataaac ataataaaaa actctaaaca tgagaaggtt
     1321 tttaacgtaa ggtgagagga tttttaagtg tatccataga ttaaaaatga caatgcctcc
     1381 aattagttga attaggggtt ataatacccg cttttaaggt tctttcatgg tcacagttaa
     1441 ccttcattgc ccccgttgcc agtccgttca ggtttaccgt cacgggcaga acccaaaagg
     1501 acatgatcga ttccgttgcc gtgactgtca ccgggtgttt cagctcactt actgttacga
     1561 ggcccgtaaa ccgggagtta aaaaccaaat cactgaaatg gctttcaacg gtgccggagt
     1621 tcgcgatact gcccgtacgc tgaaaatcgg catcaatacc gtcatacgga cattaaaaag
     1681 ctcccgcccc ggcggataac atctgcgcca gtcgctcacg ctgatgttgc ccttatctgt
     1741 gaacttgatg ggcagtggag cttcgttggc agcaaggcgc aacaacactg tcacgaacgg
     1801 tgcaatagtg atccacaccc aacgcctgaa atcagatc
//
LOCUS       DAETQN010000305         1822 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.312, whole genome shotgun sequence.
ACCESSION   DAETQN010000305 DAETQN010000000
VERSION     DAETQN010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.312"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..77
                     /locus_tag="K0W68_004631"
     CDS             <1..77
                     /locus_tag="K0W68_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBI3075943.1"
                     /translation="AARLDKIRRQNRIKAELQAVLDEK"
     gene            207..419
                     /locus_tag="K0W68_004632"
     CDS             207..419
                     /locus_tag="K0W68_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBI3075944.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            663..1124
                     /locus_tag="K0W68_004633"
     CDS             663..1124
                     /locus_tag="K0W68_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBI3075945.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWMHRK"
     gene            1170..1379
                     /gene="hha"
                     /locus_tag="K0W68_004634"
     CDS             1170..1379
                     /gene="hha"
                     /locus_tag="K0W68_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBI3075946.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            1418..1663
                     /locus_tag="K0W68_004635"
                     /pseudo
     CDS             1418..1663
                     /locus_tag="K0W68_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1678..>1822)
                     /locus_tag="K0W68_004636"
     CDS             complement(1678..>1822)
                     /locus_tag="K0W68_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3075947.1"
                     /translation="YTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIK
                     HYQ"
BASE COUNT          535 a          364 c          471 g          452 t
ORIGIN      
        1 cggcagcgcg tctggataaa atccgccgcc agaaccggat aaaggcagaa cttcaggccg
       61 tgcttgatga gaaataaaaa aaagcctcca tcagagagga ggcagggaaa taaggcttaa
      121 aaggaaataa cttccgcttc cgaaaggaag ccagtccact gaattattta tcacaataat
      181 actgacaact aaagggggac aagaaaatga atggattcag aaacagttca cggaacggcc
      241 aggtctggcg ttaccagcgt gccggcgggc gggccgttat tctggaagtc agtggacgct
      301 ggatggaagc ggcagaagca tggcgacggg ctgcctgtgt tgcgcccagg acagactggc
      361 agcagtttgc ccgaaaaagg gctgaacact gccaccggcg ctgccgggga agggtgtaac
      421 gaaaaaagca gccttcatta ctccaggagt cagaaggctg aaaggagttt atgagatttg
      481 cttcttaata tccggtcggg cagtatgacc ggacagttct gagtagcaca gagtcttttc
      541 cgcattaagt cgatttttta tattttgtga ttaatttcac aaaataaggt gttattcaat
      601 gtgtgctgcc ctattcaggg gggctgaaat acaggtgcca tttatctgat atggagaata
      661 acatgaggaa atatattcca ctggtattat ttatcttttc atggccggta ttgtctgcag
      721 atattcatgg acgggttgtt cgtgtactgg acggtgatac catcgaggtc atggactcac
      781 tgaaggcggt gcggatcagg ctggtaaata ttgatgcacc ggaaaagaaa caggattatg
      841 gccggtggtc aacagacatg atgaaatcac tggtcgcagg aaaaacggtt accgtcacat
      901 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
      961 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
     1021 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
     1081 atgctgatcc ggttcccccc tggatatgga tgcaccgtaa ataaagcgat ggcaatccta
     1141 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
     1201 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
     1261 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ttagcggaat
     1321 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
     1381 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
     1441 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
     1501 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg accggtgatg cagagcgaat
     1561 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
     1621 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tgggtaatgg ctccaactta
     1681 ttgatagtgt tttatgttca gataatgccc gatgactttg tcatgcagct ccaccgattt
     1741 tgagaacgac agcgacttcc gtcccagccg tgccaggtgc tgcctcagat tcaggttatg
     1801 ccgctcaatt cgctgcgtat at
//
LOCUS       DAETQN010000306         1816 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.313, whole genome shotgun sequence.
ACCESSION   DAETQN010000306 DAETQN010000000
VERSION     DAETQN010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.313"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(8..859)
                     /gene="hchA"
                     /locus_tag="K0W68_004637"
     CDS             complement(8..859)
                     /gene="hchA"
                     /locus_tag="K0W68_004637"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HBI3075948.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            1115..1310
                     /locus_tag="K0W68_004638"
                     /pseudo
     CDS             1115..1310
                     /locus_tag="K0W68_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1450..>1816)
                     /locus_tag="K0W68_004639"
     CDS             complement(1450..>1816)
                     /locus_tag="K0W68_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBI3075949.1"
                     /translation="MMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYVY
                     SKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDFY
                     DNNGIATDDVVGVGLVYQF"
BASE COUNT          478 a          369 c          444 g          525 t
ORIGIN      
        1 acagggatta atccgcataa gctgccagca tttcctgcgc cgccagttta cccaacgcat
       61 tcgctgcgaa aggactgtcg ccggtgagaa ctttacggtc cttatgtact cgcccggtga
      121 tgtcgtcatt aataatgttc atgcccattt tcttcagctc ttcgccgaag taccaggtga
      181 gatgacccgg catatagcct atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc
      241 aaatggaata accattcagt gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc
      301 cgtggcaaag ggagataaca aaacggtcat ttttgattgc ccactgcaaa gcagcagcta
      361 cgtcctggct ttctggtaaa ccaataagtg cgccatgacc accaggaaca aagattgctg
      421 catattcgct atcagcgttg aggctggcga caacatccgc gagtttcttc ggattgcgga
      481 acaacgattt atgctgctca aagaatggca ttactttttc atctttgtgc ggcatagccc
      541 agtattcaaa cttggtcatc agaccggaaa tggtcgccac ttcgaattcg aaaccagcag
      601 catggagatg atacaacggc agcaacgttt caatcggatg gttaccggtc gagaacagtt
      661 ttccgttatc ggttggcaaa taacgttcgt ccgcggcgat caccagaatt ttatgcttac
      721 cgcgatacgg ttttggatag tccacgccat caagatcaga gacgggactg gtatattggc
      781 taagcgaata ttctgaaggg aagaatgcat tatcttcagc aatatcgacc tgcggatttt
      841 tacttgtttg aacagtcata gtgtattcct tattgttgct tagttagggt agtcactata
      901 tttgcgactg agctggtcgc tatttttaac cagtctggaa aattgagtca atatatcgaa
      961 agcggtagtc ggcgggtgta agtatgtaat gatgatgctg cactggtgtt gaatattatt
     1021 cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg gcctcaatga ttttaggcca
     1081 ttatcaaagg tacggggcgg cggggagaga tttagtgctg tgaaatattg tggtactgat
     1141 gaatatttat atcgcggatt ggtagagaaa agattatttc atgcactatt tttgcaaata
     1201 tttctatatg ggaagcgtgt ttgttacgga ttaattcata aagaaatgca tttgggtgtt
     1261 ggtttgaaga gtcgctggtt gatgttattg tgccaggagg ggcagggtaa ggtgctgtat
     1321 cgatatgcat tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaagcct
     1381 gaattcgatg agggacgaac tcaggctagt gtttagtttt acagtaacac gccgcagcac
     1441 gttggctgat tagaactggt agactaagcc cacaccaaca acgtcatctg ttgcgatgcc
     1501 attgttgtca tagaaatcgt catctttatc caacaagttt attttatagt caacatatgt
     1561 tgagaagttc ttattgaaat agtaggtcgc tccaatttca acatagttga ccagatcctg
     1621 atcaacatac tcctgatgac cattgttgtt atagtcgtta ccgccgaggt ctttgccttt
     1681 tgaataaaca tatgcaagag atgggcgcag accaaaatcg aactgatact gtgctacagc
     1741 ttcaaagttc tgggttttat ttgcgatacc cacattacca tatggtgtca tattgcgggt
     1801 ttcagcatac atcatt
//
LOCUS       DAETQN010000307         1731 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.316, whole genome shotgun sequence.
ACCESSION   DAETQN010000307 DAETQN010000000
VERSION     DAETQN010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.316"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            71..166
                     /locus_tag="K0W68_004640"
     CDS             71..166
                     /locus_tag="K0W68_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBI3075950.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            292..1029
                     /gene="erm(B)"
                     /locus_tag="K0W68_004641"
     CDS             292..1029
                     /gene="erm(B)"
                     /locus_tag="K0W68_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBI3075951.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            974..1144
                     /locus_tag="K0W68_004642"
     CDS             974..1144
                     /locus_tag="K0W68_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HBI3075952.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFCKFGKLHVTKGNVDKLLGIL
                     LTASKELKRSLD"
BASE COUNT          642 a          287 c          277 g          525 t
ORIGIN      
        1 aattttgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
       61 gggattcgtc atgttggtat tccaaatgcg ttatcaaatg cgttatgtag ataaaacatc
      121 tactgttttg aaacagacta aaaaaagtga ttacgcagat aaataaatac gttagattaa
      181 ttcctaccag tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc
      241 gtttaacttc tgtatttgtt tatagatgta atcacttcag gagtaaatta catgaacaaa
      301 aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca aataataaaa
      361 caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa agggcattta
      421 acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga cagtcatcta
      481 ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat tcaccaagat
      541 attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa tattccttac
      601 catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc tgacatctat
      661 ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac actagggttg
      721 ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga atgctttcat
      781 cctaaaccaa gagtaaacag tgtcttaata aaacttaccc gccataccac agatgttcca
      841 gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt
      901 caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt aaacaattta
      961 agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt atttaacggg
     1021 aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta ctaaagggaa
     1081 tgtagataaa ttattaggta tactactgac agcttccaag gagctaaaga ggtccctaga
     1141 ctagcaagaa gtacacaaga agccttaaag attatagaaa agcttttcca tgaaatgctt
     1201 aacattattt aatgttaaca tgtggctttg tggtaactac cacgagtggt aacttgtaac
     1261 atgaaaaaag ttacaagtta caagtggttt atttacgaac ataaaggcta ggaaagcact
     1321 tgataagtat atcaagtgct ttttttccgt tcgattttac tatccaagat accgcttgac
     1381 gtacctaata ttttagtgtg ctaagggctt tgctttttct ggttatagtt cgttcaaaaa
     1441 agaccgcaag ggtaaatgca cgatcataga tcgcccttgc agctttttaa tctctccact
     1501 tttttataac caagcaaacc accaagcaca ctgaaaaaat aattaggaac taaagcgaag
     1561 tgcgtatctt ggataaatct tcactcgatt ttaaaagatt aagcacccag ccaaagcgcc
     1621 taaaaaaata atttttttaa agaaaggaag taatttaaat gactacaatt ttaagcgact
     1681 acaataaatc tatcctaatt gaattactaa acaccaatcg ccaaattatc g
//
LOCUS       DAETQN010000308         1710 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.317, whole genome shotgun sequence.
ACCESSION   DAETQN010000308 DAETQN010000000
VERSION     DAETQN010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.317"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..95
                     /locus_tag="K0W68_004643"
                     /pseudo
     CDS             1..95
                     /locus_tag="K0W68_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            204..472
                     /locus_tag="K0W68_004644"
                     /pseudo
     CDS             204..472
                     /locus_tag="K0W68_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            862..>1710
                     /locus_tag="K0W68_004645"
     CDS             862..>1710
                     /locus_tag="K0W68_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBI3075953.1"
                     /translation="MIKSTNIQAIGSSIMHQINNIYSLTPFSLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
BASE COUNT          609 a          341 c          270 g          490 t
ORIGIN      
        1 aagcggctgg cacgtaaaac aatctgcttc tcgcgttccg tggaaatcca cgaaaagtca
       61 tcggctcctt cattgaaaaa cacatattct actgattgga agcgccaccc gaaaaagtat
      121 cgttttttta cagtaaactt catttagccg actataatgt aaaaaaataa attttttacc
      181 aaaatataac ataaggaagt aatatgctta cacaaactat attcccttgt cttcccaaaa
      241 aataagaaaa tatcatccct gaagtatcaa atactatatt attatcttct actgttacta
      301 cagatggata tactgttttt aataaaaaaa aacagccata tacgaattgc aaatacctgc
      361 taccaaccga acgaaaaggt tgaaattcac agctacagaa atacaatggt tgacaaagat
      421 aaatgaagcc ggaattgata aaaaacaaag ccaacggtat tctgatttct gaagttaatt
      481 tcagactcgt ttaagctcta ttttcaaaaa aatatatcag ggtttgttac taataacttc
      541 tgaaaacaga aaaaaataga gcagtaattc tatttcagag aggagtaact ccatctatcc
      601 tcgatacgta ctgaacgtta tcgtgatata ttcatcatgc tttcttcacc attattcgag
      661 tacagggaga gacgcaatga ccttaaatac ctttttatgg ctgaattacc attctcagga
      721 ataacagcag actccttccc atagacggct tagggggaaa gcaatacccg gagaaagggt
      781 acaaagcaaa ctgttttaaa attaataagc tatattctat ttcactagaa ttcttatcat
      841 tgataagaaa ttttagttat aatgattaaa tcaaccaata tacaggcaat cggttctagt
      901 attatgcatc aaataaacaa tatatactcg ttaactccat tttctttacc tatggaactg
      961 actccatctt gtaatgaatt ttatttaaaa gcctggagtg aatgggaaaa gaacggtacc
     1021 ccaggcgagc aacgcaatat cgccttcaat aggctgaaaa tatgtttaca aaatcaagag
     1081 gcagaattaa atttatctga gttagattta aaaacattac cagatttacc gcctcagata
     1141 acaacactgg aaataagaaa aaacctatta acacatctcc ctgatttacc accaatgctt
     1201 aaggtaatac atgctcaatt taatcaactg gaaagcttac ctgccttacc cgagacgtta
     1261 gaagagctta atgcgggtga taacaagata aaagaattac catttcttcc tgaaaatcta
     1321 actcatttac gggttcataa taaccgattg catattctgc cactattgcc accggaacta
     1381 aaattactgg tagtttctgg aaacagatta gacagcattc ccccctttcc agataagctt
     1441 gaagggctgg ctctggctaa taattttata gaacaactac cggaattacc ttttagtatg
     1501 aacagggctg tgctaatgaa taataatctg acaacacttc cggaaagtgt cctgagatta
     1561 gctcagaatg ccttcgtaaa tgttgcaggt aatccattgt ctggccatac catgcgtaca
     1621 ctacaacaaa taaccaccgg accagattat tctggtcctc gaatattttt ctctatggga
     1681 aattctgcca caatttccgc tccagaacac
//
LOCUS       DAETQN010000309         1693 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.318, whole genome shotgun sequence.
ACCESSION   DAETQN010000309 DAETQN010000000
VERSION     DAETQN010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.318"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..799
                     /locus_tag="K0W68_004646"
                     /pseudo
     CDS             1..799
                     /locus_tag="K0W68_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            914..1255
                     /locus_tag="K0W68_004647"
                     /pseudo
     CDS             914..1255
                     /locus_tag="K0W68_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1213..1693
                     /locus_tag="K0W68_004648"
                     /pseudo
     CDS             1213..1693
                     /locus_tag="K0W68_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          424 a          409 c          499 g          361 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc agtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggg cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggccagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtcaggc ccggatgttc atcagttaca gcgaagtcag cataaaaaat
     1261 cccgacaaca gcggccaggc gctcccgctc acgtatgtct gttgccggga gcaggcagag
     1321 gacggtgcct gctggcatct gctgacgtca ggaaaagcgg cgagtgccgc cgatgcacga
     1381 cgtattgtca gccattacga gcgacgctgg ctgacagagg aataccacaa ggcgtggaaa
     1441 agtggtggta catggaatcg ctgcgaatgc agacccggga taaccttgag cgcatggtgg
     1501 ttatccaggc gtttatagcg gtacgggtgc tgggcctgcg gcagggaggt gtcagtgaag
     1561 aaacacaaaa tgacagctgt gagaaaatac tgacgccgac agagtggaaa ctactgtggg
     1621 ttaagctgga aggaaagccg cttccggtgc aggcaccgac cctgaaatgg gcctggggcg
     1681 atggcatgac agc
//
LOCUS       DAETQN010000310         1687 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.319, whole genome shotgun sequence.
ACCESSION   DAETQN010000310 DAETQN010000000
VERSION     DAETQN010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.319"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(784..1455)
                     /gene="queE"
                     /locus_tag="K0W68_004649"
     CDS             complement(784..1455)
                     /gene="queE"
                     /locus_tag="K0W68_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBI3075954.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1594..>1687
                     /locus_tag="K0W68_004650"
     CDS             1594..>1687
                     /locus_tag="K0W68_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075955.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
BASE COUNT          551 a          343 c          302 g          491 t
ORIGIN      
        1 atcgcactct atcaattttc acttaaagta tgaactggat atctttattc ataagaacta
       61 ttcatcaaca gcgtatagag gcggttatgt aaaaccactc gttagcctca aaacaaagac
      121 aaatttccag atttattact taatcaatga ataacaagct taaaaaacaa actattaacc
      181 taaagaaaaa attaacagac attaattcaa tattaaaaaa ataatagatt aaaatttctt
      241 aacgatttaa gaatcataca aataacactt tgattaaatt taattttttg tatcgataat
      301 tgtgaacctc tctggcatgg agaactattt tgaacatgag gtgttacgtg gatattttgc
      361 ttattacaag tactgctaat ataaaaactt gagaaagaga taacgggtta tatggtggtt
      421 tatccccgct ggcgcgggga acactctaaa cataacctat tattaattaa tgatttttta
      481 agccagtcac aatctaccaa ctttacagta tcacacaaac aacacatcca ttatgttaaa
      541 gagtacttaa ttcattgatt aaaaaggtaa atatttaaaa taactctata caaattaaaa
      601 tctaccaaac tttaccgcaa taattttcac tccagcgaaa aattaatgcc acagaatttg
      661 tagagaatgc taatgattag ccctggatgg gtaaaatgga ggagttttca gaggcgacaa
      721 acaatataaa tgagtagaag tctttacagg ttccacccat ttataacgct tataaatgtt
      781 taatcaggca atatttagat atttatgtgt ttgcatcgac aaacgccaat tacgcgcaat
      841 gcaggtttca atgcacaaac gtgtggcatc atccttttgg ctaatcggct gcagtgcaat
      901 gactcgcggt ttatcatcgg tcagcgttgc caatagttca tccagtgctt caatatcgcg
      961 tacgcgcccc actggatgct tgatttcgtt ggctcgctcc agtgcctgtg acaacacgtc
     1021 atagccgccg cgcatgttca gctttggcga tacggtaacc caggtattcg gtgtgcagcg
     1081 tacctcatga gtaccactgg tttcgatctg gcagctaaaa ccgttctttt cgagcagatc
     1141 agtcagtggt agcaaatcat gaatgcaagg ctcaccaccc gtaatcacca catgccgcgc
     1201 ggtgtatccc tggcgactaa tgacagccaa caagtcttca ctgctcgccg ccccccactt
     1261 atcactctct ttggtcttcg ccagaatgct gaaaagggag acttcccgat cctcaagctt
     1321 ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc gggcatccct gtaaacgaat
     1381 aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc agggtctgga acatctcgtt
     1441 aatcgggtac tgcatagcat tctctgtgaa gtggataatt gttaattatt gcagatcctg
     1501 ccacaacaat catgtcttat taacattctg ttacaggcag atttaaagaa aaatgcgaaa
     1561 catatcgtta ataattaaag ggagtaacgt attatgtcag aagaaaataa agaaaatgga
     1621 tttaatcatg tcaaaacatt caccaaaatt atatttattt tttctgtatt agtttttaat
     1681 gataacg
//
LOCUS       DAETQN010000311         1679 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.320, whole genome shotgun sequence.
ACCESSION   DAETQN010000311 DAETQN010000000
VERSION     DAETQN010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.320"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..889)
                     /locus_tag="K0W68_004651"
     CDS             complement(<1..889)
                     /locus_tag="K0W68_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3075956.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHP"
     gene            complement(989..1345)
                     /locus_tag="K0W68_004652"
     CDS             complement(989..1345)
                     /locus_tag="K0W68_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBI3075957.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(1481..1588)
                     /gene="rprA"
                     /locus_tag="K0W68_004653"
     ncRNA           complement(1481..1588)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="K0W68_004653"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          539 a          334 c          393 g          413 t
ORIGIN      
        1 tgggatgcag gcagacggta aacagagcaa taaatgagat aaaggtgtac agcatcagta
       61 atgttgttgg gcgaacggta ttacgaatga gtggagcggt aataaataca caaagcagcg
      121 aacccacggt gtagatactg agtagtttga tcgacatagt gtatgacatg cctgcaacaa
      181 attgtccgta ctgtgccagc cactggctaa ccagataaaa cgttgccatt gagatatagc
      241 catataaggt ataactggct aaatcgataa ttgaacagcg atgttccgta gagctggtgg
      301 ttttctttat gacaggtaag cgacgacccg gatggggtgg gaacgtacaa cggtataaaa
      361 acagagcgtt aataaacata atgcctgcag caatcataaa ggaccaaccg aaccacagtt
      421 cagcccacac taacaggcta atgattagcg gtaataaaaa ttgtccgctg gaaacaaatg
      481 ctttaattaa aatattggct gtcccaggtg agcgtggaaa agcttccatc aaactgggat
      541 aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag aaagccaaaa acataagcga
      601 taatgatgtt attggtctgc aggatgccaa aaaagaaggc catatagcag cacatcccga
      661 gcatgataaa agggcggcga ccaaagcgat cggataataa tcctgcaaaa agcaagacac
      721 ttaatcgacc aatgcccagc gatgagataa ctatcgagac acccgcggca ttagtctgcc
      781 aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat gacgcccata ccatgcacca
      841 ggtaattaaa atacaaccca agtgcggtag ggtaataggg atttttcata ggttctactt
      901 agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt atccattcat tattgttgta
      961 acaacgacaa ttcctttctg tggaaaaatt aacaaagccg ctccagatcg tgtgcatata
     1021 attcagcggc taccgattga tagatcttcc agtcaataaa attgccatca gggtagactt
     1081 gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt gttgccgatg cggttattta
     1141 ttttctctat gatggcatga agatgttttt ttcgttgctg acgcatagcc aacagttgct
     1201 cgactacaca atcaggaatg gcgcatttgc cattttccca ctgacgccag ctttcactgt
     1261 caccggtctg ggcaatccag gtcgcgcatt catcaatagt catggcaaaa atatggcgga
     1321 gtgcctggag ttcataagcg ttcatcgtgt aatggctgca tgaaataaac aataaaatat
     1381 aaataacatt atcagcctgc tgacggcttg aagagagtca cagtatcttg tgcaacatta
     1441 ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg ttaaaaaaaa gcccatcgtg
     1501 ggagatgggc aaagactaca cacagcaatt cgttgtttca ctcaggggat ttccatgctt
     1561 ataaatcaat atgttgattt ataaccgtga gctaatagta ggcatacgga ctttttgcgt
     1621 cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat ttgcgacagg tactaccag
//
LOCUS       DAETQN010000312         1667 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.321, whole genome shotgun sequence.
ACCESSION   DAETQN010000312 DAETQN010000000
VERSION     DAETQN010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.321"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(183..604)
                     /locus_tag="K0W68_004654"
                     /pseudo
     CDS             complement(183..604)
                     /locus_tag="K0W68_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(588..815)
                     /locus_tag="K0W68_004655"
     CDS             complement(588..815)
                     /locus_tag="K0W68_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3075958.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYAPKVYDEYRKAKRPWKVIHENQA"
     gene            892..1299
                     /locus_tag="K0W68_004656"
     CDS             892..1299
                     /locus_tag="K0W68_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3075959.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1503..1658
                     /locus_tag="K0W68_004657"
     CDS             1503..1658
                     /locus_tag="K0W68_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBI3075960.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT          470 a          436 c          344 g          417 t
ORIGIN      
        1 cttccctaat gcggcatctg gatcaatatt cagaatctca gcaagcctga atatttccgg
       61 cttatccggc gtaataacct cgagctttat ccagtttgaa gccatttgtt ttcaccttgt
      121 aacgctcgca gcgttacatt taactgatac cgaacaaaac aatccggcac gattaatttc
      181 aatcaatgca ctacgacaga atcgccgggc gacccaccac cgctgaaatg tgctttccgg
      241 taaacggcct ggacagcatc atcatgcgca tcaattgccg tactcaacgc ttcctgcgcc
      301 gccagtaatg cacggcgttc cagggtatcg aagatacaga gtcggtgacg cagctcacgc
      361 ggaagaattg ccagaaccgc agggatcagt ttctgaattt tttccctttg cgctttcgtt
      421 tcacctttca accaacggtg atagatattc tgctgattgt tccagtcctt gcctggtacc
      481 agagacaatt cgccgccccc ctgccgcaga tattcttcag taattgcgtt agcgacccac
      541 gcctgccctt tttcggctgc cagggcaaca gcactgattc gatgtgctca tgcctgattt
      601 tcatgaatca ccttccaagg tcgttttgcc ttacgatatt cgtcataaac tttgggggcg
      661 tactgaagtt ccccgccaga tgcctcttgc aggcgcatcg cgcgaccttc aggaaccagt
      721 tccccccatg cagcaatgct tgccagccta actcctgcgg cattggcaag ctttgttttg
      781 ctgccaaaaa acgctatagc atcaattttc aacatatcga gctccttaga ttttcctaag
      841 gaaactagat cgtagagaaa cctaagtcaa gaaaaattag aattccctaa tatgaaaaac
      901 gaaaccttcg gtgctcgcct cttacatagg cgtaaaaaat taaaactgtc tcaggccgca
      961 ttaggtaagc tggtcaaagt ggctcacgta acaatttctc aatgggaaag agatgaaaca
     1021 cagccggcgg ggaaaagatt attcgcactg agccaagcgc ttcagtgctc accgacttgg
     1081 cttctttttg gggatgaaga taaacaacca ggcgaaccga tcccggataa tcagccagcc
     1141 attctgacag aagatcaaaa agagttactt caactgttcg acgcactgcc tgagtcagag
     1201 caaaaggccc tgttgtcaga gatgcgtgct cgagttgaaa atttcaacaa actttttgaa
     1261 gaactactca aagctcgcaa aagaagcgca aacaaataat cccctttttt ctccacaccc
     1321 tgtaataaaa agcacaaact ttcaaacact tgtgtttttt acatcaaaaa aacttaggtt
     1381 tttctacaca aaaatcttga ccgcccacct taggttattc taaatttcat tcatcaagac
     1441 accgcacggt gttctcagca aacagttccg ctaccccggc gttaagggga aatggggtca
     1501 acatgaatac tatcgatctt ggcaacaacg aatctctggt gtacggcgtg ttccccaacc
     1561 aggacggcac attcaccgcc atgacgtata ccaaaagcaa aacgtttaaa accgaagctg
     1621 gcgcacgtcg ttggttggga agacattcag gtgagtaaaa tgaacga
//
LOCUS       DAETQN010000313         1658 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.322, whole genome shotgun sequence.
ACCESSION   DAETQN010000313 DAETQN010000000
VERSION     DAETQN010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.322"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..127
                     /locus_tag="K0W68_004658"
     CDS             <1..127
                     /locus_tag="K0W68_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI3075961.1"
                     /translation="CYTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVENGQHQ"
     gene            553..1131
                     /locus_tag="K0W68_004659"
     CDS             553..1131
                     /locus_tag="K0W68_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075962.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1240..1498)
                     /locus_tag="K0W68_004660"
                     /pseudo
     CDS             complement(1240..1498)
                     /locus_tag="K0W68_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1482..1658
                     /locus_tag="K0W68_004661"
                     /pseudo
     CDS             1482..1658
                     /locus_tag="K0W68_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          541 a          291 c          375 g          451 t
ORIGIN      
        1 ctgctacacg ctgaatatca aaggagagag ttaccggtta aaagagaaac gtaaagctgg
       61 agtgctgacc aaaaacacaa cgccaatcag tgatgatgaa atggtggaaa acggacagca
      121 tcagtaacga aagtatctta gcaggcatga aaatcgtccg catcccctaa acggtcaaaa
      181 tgcttctgcc gtgaaggagg gagaatgcca tctcatcact tatatctggc gaatgaaaca
      241 cagatggtcg gccttgcccc caaacacacg gcggtgcaaa atggaatggc agaaaacttc
      301 gtcaaaacga tgaaatatga ttaaatcagt gttatgttcg aacctgacgt gttaacagca
      361 gtaaagtagt ctcgcgaagg tgttcaaaca ttataatgaa tggcatcctc atagtgcgca
      421 gggttatcgc ttgctgcgga aatatctgaa gcgacgtacc agtaatgggg aaaatgatag
      481 ctggtttctg aaaatataga ggaaaatgtg atatatatat cctgttaata tttaaggaga
      541 atataaaata aaatgaaagt ctcgtttaag tctttaggct atatttttca tgatatatat
      601 aataaaaaac acactatcga tgagttcaac gatgtggtaa gaaaagcagt tttatcaggt
      661 aaaattaacg agctgaatgc atgtcataaa gtggcaattt ttcttgccga aaaagacaat
      721 gagataacaa agaaagacaa agccaagata atagatacat taactgaaaa ctattccatt
      781 gaatttcaac aattaatgaa tattagtgag cgcacgttaa attcatcttt atatattact
      841 cctggtgaaa gtggttttgt cagttttgtt aatcgtgaag gaaaaatatg tcatactgca
      901 tatgtaaaat catcagataa tagcatggca tattaccatg caaattatag cagcattgac
      961 aaatatatta cggatatgtg cggccttatt tgtatgaggc atattgagtc tactggcatt
     1021 attttttata tgcttgatga aaaagtttta tctgccattg ctgaatttat gaatgaaaaa
     1081 ggatggcggg ctgcgttttg ttcagcaaag aacttatata aatgtgttta attttagaaa
     1141 aactatttgg taaaatccaa caagagtcga gaggttcgcg ttgcttcagc agggcctcag
     1201 cctgttcaac cagactcagt tgcaggtcgt gacgcagatt tagtggtggt ggttgattgt
     1261 cagataccag ccgatgatct tgtagtgcat atcatcagac tttgagaagc atatggtttt
     1321 gcgggtaagc cgcttaagat gtgtccgcag gttcagatta tgtcgctcta tccgctgagt
     1381 gcatttcttg cttataacat gactggttga ggacaataaa gttcggtata ccggctcagg
     1441 catcggtcat gtagaaggca atgctaaact ggctcagcaa actaagagca gcgcgcatcc
     1501 acttacccaa tgcggttgag aaaatagcct tcgatcgctt tcatgtggcg aaacaaccgg
     1561 gtgaggtggt ggataaaacc cgccagaatg aacatccgca tcttcctgtt gaaagtcgtc
     1621 gtcaggcaaa gggaactcgc ttcctgtggc agcacagc
//
LOCUS       DAETQN010000314         1624 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.323, whole genome shotgun sequence.
ACCESSION   DAETQN010000314 DAETQN010000000
VERSION     DAETQN010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.323"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..266
                     /locus_tag="K0W68_004662"
     CDS             <1..266
                     /locus_tag="K0W68_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3075963.1"
                     /translation="GLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSR
                     AYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTFYTPQGS"
     gene            complement(637..903)
                     /locus_tag="K0W68_004663"
     CDS             complement(637..903)
                     /locus_tag="K0W68_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075964.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEVGIVEKQSQRHSNI"
     gene            complement(1085..1483)
                     /locus_tag="K0W68_004664"
                     /pseudo
     CDS             complement(1085..1483)
                     /locus_tag="K0W68_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          403 a          362 c          353 g          506 t
ORIGIN      
        1 ctggccttgc tccggtaact cggcgatccg gctcgtcaat acgcggtgag catccctcgc
       61 gacggggtaa taaagctctc aaacgggcgt tgttcctgtc ggccttcgcc gcgctcaggg
      121 atccgctctc cagggcttac tacacccgca aaatgagtca gggaaaacga cacaatcagg
      181 cgcttatcgc cctggcgaga cgacgctgcg acgttctgtt cgccatgatg cgcgacggga
      241 ctttttatac cccgcagggg tcataacatg cttgacaact taatagggcc ccccccctaa
      301 gccgtctatg ggaaggagtc tgctgttatt ccagagaatg gtaattcagt catatatttc
      361 tccatctatg acttgtcaat accatcctga tatttgtggg tcagcaatat attctactaa
      421 aaggtattta agatcattgc gtccctgcac tcgaataatg gtgaagaaag catgatgaat
      481 atatcacgat aacgttcagt atgtatcgag gatagatgga gttactcctc tctgaaatag
      541 aattactgct ctactttttc tgtttccaga aattactagt aacaaaccct gatatattct
      601 tttgaaaatg gagcttaaac gagtctgaaa ttaacttcag atattagaat gccgttggct
      661 ttgtttttca acaattccta cttcatttat ctttgtcaac cattgcattt ctgtagcagt
      721 gaatttcaac gttttcgtcc ggttggcagc aggtatttgt aattcgtata tagctgcttt
      781 tttattgaaa acagtatatc catctgtagt aacagtagaa gataataata caggatttga
      841 tacttcaagg atgatatttt cttgtttttg tggaaggcaa gggaatatag tttgtgtaag
      901 catattgctt ccttatgtta tattttagta aagaatttat tttttacatt atagtcggct
      961 aaatgaagtt tactgtaaaa aaacgatact tttttcgtaa gcgatgagat gtggagccag
     1021 aaaaatggct gcgttacgtc attgaactgt ccttgaccca cgatattcag acagtcgttg
     1081 tatcttaagt tgacattacc cgctgacgtc gatattccct tggtgagaga taacccaacg
     1141 cactatgcgg atggttttca ttgtaatgct cgatcgccac tgcaaggtta tgcaatgccg
     1201 ttcttggttt cggcatgaac gcgatgcagt cttccttcat cgttttcacg aaccgttctg
     1261 ctattccatt actctgaggg ctactgactg ccgtcgtgca gggctccaga ttcaactctc
     1321 tggcgaactg ccgtgtttca tgagccgtat aagccgaacc gttatcggcc actggattgg
     1381 ccgttccggt cctttatcgc catatcgctg acatgacatc ctgcaccgtt gctttgtcat
     1441 aatctcccgt gcctgctgcc cagtctatgg cttcacgatc gcaatatgga acgtaacgct
     1501 gtcatcggga gaatcgtcaa tgtggcattc ggttaacgct gatatgactt tccgtgtcaa
     1561 accggaatct ctaattttga cgattttctt tttaggttta attccctgag ggcgacgtag
     1621 cagt
//
LOCUS       DAETQN010000315         1618 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.324, whole genome shotgun sequence.
ACCESSION   DAETQN010000315 DAETQN010000000
VERSION     DAETQN010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.324"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..160)
                     /locus_tag="K0W68_004665"
                     /pseudo
     CDS             complement(1..160)
                     /locus_tag="K0W68_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(159..>1618)
                     /locus_tag="K0W68_004666"
     CDS             complement(159..>1618)
                     /locus_tag="K0W68_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HBI3075965.1"
                     /translation="KGIVNISTDSLWNLKTSSTNAQLLQVGVLGTGELNITTGGIVKA
                     RDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLNVEGGEVYLGV
                     FEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSDAGYQVDMLIT
                     GDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMGSSEVTIASPG
                     TLDILASTNSAGDYTLTNALKGDGLMRVQLSSYDKMFGFTHATGTEFAGVAQLKDRTF
                     TLERDNTAALTHAMLQSDSENTTSVNVGEQSIGGLAMNGGTLIFDTDIPAATLAEGYI
                     SVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPIANNPLTTLNLLEHDDSHVG
                     VQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGFRLTTAPGDGL
                     YVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAK"
BASE COUNT          364 a          464 c          362 g          428 t
ORIGIN      
        1 gcaatacata gcggttagcc tgagtattta gctggccgtc accggtcctg aaacgttcgt
       61 gccccccgac atgacgcatc cacatactgc ttgccgatcc ctgagaatgc agtgaatctg
      121 tatactgcgg ctcacccaga cggtcgtgta agcgatggct attttgccga catatcggcc
      181 gttgcgccat aggctccgcc atgttcggct aacgtcagct tttgcccacc atggatgttc
      241 agcgctttca gcccatagtt aacgtacaaa ccatcacctg gtgcggtcgt gaggcggaat
      301 ccataatcac cctcggcgac caccgttccg ttttgcgcaa tgtgtaacgt tttgtccgct
      361 tccacctcgt cgccctgtaa atcacgtaac gttaatgagc cacccgaccc aataaccgtt
      421 tgcgccttca ccagttgaac gccgacatgg ctatcatcgt gttccagcaa attgagcgtc
      481 gtcagagggt tattcgctat gggatcattc cacggtttag gcacgtcgat aagcacgtcg
      541 cccgtcccgt ttacctgata gttacggcct ttccaggtgt agtcacccgc gccgacaacc
      601 agcgtatcga cgctgatata tccctctgca agcgtcgcag caggaatatc cgtatcgaaa
      661 atgagcgtac cgccattcat ggccagtcca ccaatggatt gctctcccac gtttaccgat
      721 gtggtatttt cactgtcaga ctgcaacatc gcgtgagtaa gcgcagcggt gttgtcgcgt
      781 tccagagtga aggttctgtc tttcagttgg gcaacaccgg cgaattcagt ccctgttgca
      841 tgggtaaagc caaacatctt gtcgtaggat gacagctgca ctcgcatcaa gccatcgcct
      901 ttgagcgcat tggtcagcgt gtaatctcct gcactgttcg ttgatgcgag aatgtcgagc
      961 gtaccggggc ttgcaatggt gacttcactc gaacccatgc ccgttacccc atctgccgta
     1021 tgagacgcaa tggtcaggag gccgtcattg accagcgttt taccgctata agtattccct
     1081 gcattgaaca ccgtatggcc tgcatcatgg atcacttttc cgtctttatc gtcacctgta
     1141 atcagcatat cgacctggta gccggcatca ctgttattag tatgattaaa gacaaaaaca
     1201 ccttcgccag aaccaaactc tactttcgtc gcgttggtta tatacccggc atctgccgcc
     1261 gcctcaccgt gagctgcacc aatgtttagc gttcctacag caggttcaaa aacacctaag
     1321 taaacttctc caccttcgac attcagcgtg ccgctattcg tcagggttaa cgtacccgta
     1381 ccagatgtcc ctacgtacat attgaatgtt tcaagaagag agttctgccc atccaccctc
     1441 acgtcgccct tacttttgtc attgagagct atctgtgtat cacgcgcttt aactatacct
     1501 ccggtggtaa tattcagttc acccgtgcct aatacaccga cctgtagcaa ttgtgcgtta
     1561 gtagatgatg tctttaaatt ccagagactg tccgttgaaa tattaactat ccccttac
//
LOCUS       DAETQN010000316         1557 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.325, whole genome shotgun sequence.
ACCESSION   DAETQN010000316 DAETQN010000000
VERSION     DAETQN010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.325"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            271..1386
                     /locus_tag="K0W68_004667"
                     /pseudo
     CDS             271..1386
                     /locus_tag="K0W68_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          614 a          280 c          221 g          442 t
ORIGIN      
        1 aaaaaaatca cttcacacac tatcagagaa cataataact ttttaaaatg agcgcatttt
       61 gatagttgtt aacgatttcc tttacgatac tatcactacc ctttttttat acacaaatat
      121 taccgggtgg agataaaagg gaaatcaaag gtaattatat caggaagata taacgcatta
      181 catttattgt gtgtagaaga aaatccattc ctgttctaat tatccaattt aaacatctta
      241 gcataaaaca aatgatgaat aaggaatttt atgtcaattg actttacccc aggtataata
      301 aatacatatc acggcgatat ttataactgc acaacaaata ccgataatgc taaaacgcca
      361 gacacaccga agtggccttg cgataattgg gaagaacaac aacccattaa ttccacattc
      421 tctggagaag gatatatctc tgatcaatat gatttagtgc aacaccaact tcaacaaatt
      481 aatgcgtgcc acacgaacac tacatataca aatgcagact actcaaaagt tgtggctcaa
      541 cttgtaagtc ttattactaa cattgaaacg ataagttcga cacaacttac gcaacagaca
      601 caatcaatac ttaatcagat taataacatt agatatgaaa aaaacaaaag tgctgaatgt
      661 cgaatcattg ttgtcgctaa tcctaaaccc gacaaggcaa tcataactaa aattagcgta
      721 gaagagggaa tacccataac attcagtgtc caaaccatgt tttcagatac taattttatt
      781 gctgaacaac gagctgactt accaactaat ataaaagata ttcaaagcct gtatcaaaaa
      841 atgacgaagt tatatattga gcacagtgaa aataaaaaca ggatgaaagt ctttgccgga
      901 gccaacttta ttgatttcaa tatgacagga cagaacctgt ccgggttcgt tctaaccttg
      961 tcaaggtttt atttcgaaga tctacttaat ataaatttca ctgacgcaaa tttaggtgac
     1021 actatttttt acataaagaa caccctaccc ccaaattata taaagatgga caatatcttg
     1081 acaaacaaat cgaaggttta ttttcaacat tattaaccat taatgacaac tttttacgag
     1141 caaaagcgga aattgcttcg acaataatta aatttttaga ggccaggata accaatcttt
     1201 catacaatga catattaaaa tatcagcaag aattccaaaa acaatgttat aagcaagtta
     1261 aagcctttac gacactctct cgatacaata aaattcaaac atgggcagaa atgtctgaat
     1321 atcagttcga agtatttcaa tacgagacgc ttaaccccaa aaaaatgtca cacacgcctt
     1381 atttaaagag gccacttcct aatgaaaaag atataaatta tggggtggaa atagaaatac
     1441 catctggcaa aagaattcgc ttatcaaatc attatcagaa tataatacca taatattgat
     1501 tattagcact ttataattat tgaatgaaaa tagattttat gtaattttaa aacaaag
//
LOCUS       DAETQN010000317         1551 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.326, whole genome shotgun sequence.
ACCESSION   DAETQN010000317 DAETQN010000000
VERSION     DAETQN010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.326"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..104
                     /locus_tag="K0W68_004668"
     CDS             <1..104
                     /locus_tag="K0W68_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3075966.1"
                     /translation="HLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(121..243)
                     /locus_tag="K0W68_004669"
                     /pseudo
     CDS             complement(121..243)
                     /locus_tag="K0W68_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
     gene            complement(255..947)
                     /gene="rutB"
                     /locus_tag="K0W68_004670"
     CDS             complement(255..947)
                     /gene="rutB"
                     /locus_tag="K0W68_004670"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBI3075967.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(947..>1551)
                     /locus_tag="K0W68_004671"
     CDS             complement(947..>1551)
                     /locus_tag="K0W68_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBI3075968.1"
                     /translation="GDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCF
                     GKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADE
                     EALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPG
                     AEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          359 a          425 c          427 g          340 t
ORIGIN      
        1 agcacctggc acggctggga cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg
       61 acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga gtcattaccc
      121 gttattatgt tgatcaaaag ccagcgtacc ggagacatac accacgccat cagccagcgt
      181 gccgggaacg aagggggcca gcggtgcgct gctgccagcg ggaataatta cggattttgg
      241 catcgttaaa ctccttaagc gatacgagca aaggacgtgg gagaaagcgc gtcgcagaac
      301 gtttcgacgt cgctgaccca gccaaaaaag gtttcgatat tgaacaacgc ggctttctgt
      361 gcaaattcag gccccgcctg gtgtgttgcg tcttccagca ccacgccgaa atactccaga
      421 aaaaagccat cgcgtagcgt cgattcgacg cagacgttgg tagcgatacc ggtgaaaacc
      481 agatggcgta ttccacggct gcgcaaaatg ctgtccagcg gcgtattgaa gaaaccgctg
      541 tagcgcggct tcggcagcac aatatcgcca ggctgcggca ccagttcatc caccagttga
      601 taatcccagg agcctttcgc cagcaatttc ccctgcagct gcggctgctt acgcatggtt
      661 ttcagggcgt tcgatttatg aaaattcggt gagccgggtc cgccagcttc gacatactgt
      721 tcatcccagc cattttgaaa ccagatgatc agcatccctg ccgctcgcgc tgcggtcacg
      781 gcggtttgaa tgttggcaat gaccgggcga gtggttgaga catcaaaccc ggcgagatct
      841 aagtagccgc ctggcgtggc ataagcgttt tgcatatcca ccacgatcag cgcactttgc
      901 tgcggatcga aggtaatggc ttccggtcga gcggttaagg tcgtcatcat gccacctcct
      961 gagtcagcgc agggagatgg gcgcggcact gcatcagtgg ttgaatgcgc tcgccgaagg
     1021 tttcgattcc cgacagaaaa tcatcgaagg tcaacagcac gccttcggca ccaggcacgc
     1081 ttgcgacttc atctaacatg cgcgcgacac tggcgtaaga accgactaat gtccccatat
     1141 tgatgtttac cgccgaagtg ggatcggcca tctgacggac gttggtgtca gtaccggagc
     1201 gggtatcttt ctgactttgt tcggttagcc agcttaacgc ctcttcatcc gcgcccgctt
     1261 tgtagtgttc ccatttggcg cgagcggcat cgtcggtttc atcggcaatc accataaaca
     1321 acacataaga gccaacgtcg cgcccggttt gctctgcggc ctgtttcatc cgcacagcgg
     1381 tcggggcgaa agccgtgggt gtatttacgc ctttgccgaa acagaagttg aaatcggcat
     1441 actgagcgga gaacgccatg ccagcgtcgc tttgcccggc gcagatcact ttcatgggga
     1501 cactcggttg cggactgacg cgacaatcat tcatggtgaa aaaatcgcct t
//
LOCUS       DAETQN010000318         1549 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.327, whole genome shotgun sequence.
ACCESSION   DAETQN010000318 DAETQN010000000
VERSION     DAETQN010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.327"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            351..476
                     /locus_tag="K0W68_004672"
     CDS             351..476
                     /locus_tag="K0W68_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBI3075969.1"
                     /translation="MWLPVLFRFLVDSLAMLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="K0W68_004673"
     CDS             complement(921..1397)
                     /locus_tag="K0W68_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3075970.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          359 a          405 c          384 g          401 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcaccc cgacacccca catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctatgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggttcaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atccaggatg
     1501 cgacaacgtt gtgtcatcct ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DAETQN010000319         1548 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.328, whole genome shotgun sequence.
ACCESSION   DAETQN010000319 DAETQN010000000
VERSION     DAETQN010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.328"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="K0W68_004674"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="K0W68_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HBI3075971.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..>1548
                     /locus_tag="K0W68_004675"
     CDS             1253..>1548
                     /locus_tag="K0W68_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI3075972.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSE"
BASE COUNT          387 a          388 c          437 g          336 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaa
//
LOCUS       DAETQN010000320         1536 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.329, whole genome shotgun sequence.
ACCESSION   DAETQN010000320 DAETQN010000000
VERSION     DAETQN010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.329"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(488..1012)
                     /locus_tag="K0W68_004676"
                     /pseudo
     CDS             complement(488..1012)
                     /locus_tag="K0W68_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
     gene            1267..1513
                     /locus_tag="K0W68_004677"
                     /pseudo
     CDS             1267..1513
                     /locus_tag="K0W68_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
BASE COUNT          438 a          353 c          275 g          470 t
ORIGIN      
        1 tttttccccc aaaataaaca gacattggtg catcattata agctaatgct ttacccattt
       61 tacccgcagc taaatcaact gatgtggcag aaccattatc ttcaacaaat gcagttacac
      121 gaccaccaaa gtttgctaac tgaacatcct taccacccac ctgaactttt tcataacctg
      181 tagagttttg tttcaataat gtaaaaccgt tcgtgttggt attattatct tgaccaacaa
      241 aataagtctg cccttccacc tgtacttgat aaacttttgt tcctgcagta ccggtcgcat
      301 cagttgtatc ctttgtatct tgtacattac cggtaccaaa ataagtttca acatcagcat
      361 ttttataatt gctaccgaat gctttagcac ctcccacact accatcagtt gcatttacct
      421 tagttaagtc aggagcggct tcgctcttaa gtgcaaccgc agaggacatt ggaacttttc
      481 ctagtgctac gctaaagtta tcaagcccca aggttttagc atcaatttta tcaagggcaa
      541 tctcaatggt ttcaccatcg ttcgcaccta cttgaatttt catagtctga tcagaagcca
      601 gaacttttac accattaaat tgtgtctggt tagaaacacg atcaatttca ctaaggcgtt
      661 gtgtaatttc atcctgaatt gaattcaggt cggagtcaga gtttgtacca ttagtggcct
      721 gtactgacaa ttcacgaata cgctgtaagt tattgttgat ttcagaaagc gcaccttcag
      781 tggtctgcgc aacagaaata ccatcattcg cgttacggga agcctgagtc agacctttaa
      841 tatttgctgt aaaacggtta gcaatcgcct gacctgctgc gtcatcttta gcactgttaa
      901 tacgcaggcc agaagagaga cgttcaatgg cggagctcag agaagactga gatttgttca
      961 ggttattctg ggtcaacagc gacaggctgt ttgtattaat gacttgtgcc atgatctttt
     1021 ccttatcaat tacaacttga tgttattggg ctgttgccca cggtttctca ccgtacccct
     1081 tgtatcggca cctgaatttc gaactttaga aaattttagt ttctctcctg ttttttctta
     1141 acggctgaaa tatccgtaat atacaactct actctgcgca tcatcttcgt gaatcaacta
     1201 ttaaactttg cgcacaaatt gccgataacc cgcttatcta ctgttttcaa tcaaaaggaa
     1261 ttaggtatgg cttcatttac atctcttggc gttggttcga atctgccgct ggatacattg
     1321 ctaaacaacc tgaccatcgc tgaaaaaaaa cgcttaaacc caattactca gcaacagagt
     1381 gacaataccg cacgtctgac agcctatggc acattaaaaa tgtgcattag aaaaattcca
     1441 gactgcaaat actgcgttaa ataaagccga tctgttcaaa agcactaccg ttaccagtag
     1501 caatgaagac ctttgaggta gcctgagttt aacgga
//
LOCUS       DAETQN010000321         1532 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.330, whole genome shotgun sequence.
ACCESSION   DAETQN010000321 DAETQN010000000
VERSION     DAETQN010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.330"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..1216
                     /gene="rhsC"
                     /locus_tag="K0W68_004678"
                     /pseudo
     CDS             1..1216
                     /gene="rhsC"
                     /locus_tag="K0W68_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
BASE COUNT          385 a          443 c          434 g          270 t
ORIGIN      
        1 gttacagagc cagcacctga acagcctgct gtctgaccgc gattacacct ggaacgacaa
       61 cggcgaactc atccgcatca gcagcccgcg ccagacccgg agttacagct acagcaccac
      121 cggcaggctg accggcgttc acaccaccgc agcgaatctg gatatccgca tcccgtatgc
      181 cacagacccg gcggggaacc gcctgcccga cccggagctg cacccggaca gcaccctcag
      241 catgtggccg gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccatca
      301 cggcagactg acagagaaaa ccgacctcat cccggaaggg gttatccgca cggatgatga
      361 gcgcacccat cggtaccatt acgacagtca gcaccggctg gtgcactaca cgcggacaca
      421 atatgcagag ccgctggtcg aaagtcgcta tctttacgac ccgctgggcc gcagggtggc
      481 aaaacgggta tggcggcgtg aacgggacct gacgggctgg atgtcgctgt cacggaaacc
      541 gcaagtgacc tggtacggct gggacggcga ccggctgacc acaatacaga acgacagaac
      601 ccgcatccag acgatttatc agccggggag cttcacgcca ctcatcaggg tcgaaactgc
      661 caccggtgag ctggcgaaaa cgcagcgccg cagcctggcg gatgcccttc agcagtccgg
      721 cggcgaagac ggtggcagtg tggtgttccc gccggtgctg gtgcagatgc tcgaccggct
      781 ggaaagtgaa atcctggctg accgggtgag tgaggaaagc cgccgctggc tggcatcgtg
      841 cggcctgacg gtggcgcaga tgcaaagcca gatggacccg gtatacacgc cggcgcgaaa
      901 aattcacctg taccactgcg accatcgcgg cctgccgctg gcccttatca gcacggaagg
      961 gacaacagcg tggtgcgcag aatatgatga atggggcaac ctgctgagtg atgagaaccc
     1021 gcatcatctg cagcagctca ttcgcctgcc ggggcagcag tatgatgagg agtccggcct
     1081 gtattacaac cgccaccgct attatgaccc gctgcagggg cggtatatca ctcaggatcc
     1141 gattggactg aaggggggat ggaattttta tcagtatccg ttgaatccgg ttcagtatat
     1201 agattcaatg gggctggcat ctaaatatgg acacttaaat aatggcggat atggagcgaa
     1261 acccagcaaa ccgcctacgc ccgatccaag taagttgcca gacatagcga aacaattaag
     1321 actgccatat cctattgacc aggccagtag tgcgcctaat gttttcaaaa cattcttcag
     1381 agcattaagc ccttacgatt acacactgta ttgcaggaag tgggtaaaac ccaatctgac
     1441 ttgtatgccg caggatgatc cccagtatcc agggatggag acaaagacag caagtgatta
     1501 cctgccacag acaaattggt caacaactca at
//
LOCUS       DAETQN010000322         1516 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.331, whole genome shotgun sequence.
ACCESSION   DAETQN010000322 DAETQN010000000
VERSION     DAETQN010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.331"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..>1516)
                     /locus_tag="K0W68_004679"
     CDS             complement(<1..>1516)
                     /locus_tag="K0W68_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBI3075973.1"
                     /translation="SRNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFT
                     DCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGD
                     LTAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYD
                     PVDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQ
                     WQYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAY
                     SEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAY
                     ELATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVH
                     TTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAE
                     KTDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTG"
BASE COUNT          222 a          510 c          442 g          342 t
ORIGIN      
        1 cccgtccggc ggcccagcgg gtcatagagg taccggctct ccacctgtgg ttcgccgtgc
       61 tgtatccgcg tgtagaacac caggcggtgc tggctgtcgt agtgatagtg gtgggtgcgc
      121 tcgtcgtgca tccggataac cccttccggg atacggtccg tcttctccgc cagcctgccg
      181 tattcatcgt agcgatagac atagtgcgca tcttccgcga tgcggttatc cggccacgcc
      241 gtgagggtgc tgtccgggtg cagttccggg tccggcagcc gatttcctgc cgggtctgtt
      301 gcatacggga tatcgatatc caggttcgct gccgtggtgt ggacgcccgt cagcctcccc
      361 gtgtcgctgt aacggtactc ccggctctcc tgcgggccac tgatgcggat tagctgtccg
      421 ttgtcgttcc agtcgtagtc acggtcaagc tgcggaaggt tcaggtgcct gctccggagc
      481 tggccgctgg tattccaggc ggtggcaagt tcatatgcct ccccgccgaa gctgcgggcc
      541 gtctcacggt gcagccggtc ccgcgtgtac tcgaccagcg gtgttccgcc cagcttcatc
      601 cccgcaagat aaccgctgcc atacgtcagc cactccaccg gcggcaggcc gtccggctcc
      661 tgacgggtcg ccagcccctg ttcgctgtac gcgtgccccg tctcatgctc ccacagcatc
      721 tcccccgtct ccggggtctc caccgtctgc cgttcgcccg tcagacggcc tttatcgtca
      781 tagccgtagt ggaccgccac acggtggcct tcactcaggt ggctgatttc tgtcagccag
      841 ccgtggtcgt catactgcca ctgctccgcc ggttcaccgt tcaccgtgcg ccgggtgata
      901 cggtccgacg catcgtaatg ccagagggtg ataagcccct catcttcact ctgcgtgagt
      961 tttccggtca ggtcatactg gtaacgttgc gtccggccgt caaagccgcg ctgctccgtc
     1021 agcctgtcga ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcaca
     1081 gtgatgcgcc cggcggcgtc ataccccatg cttcgcgtca gaccgccctg cgtggtgctg
     1141 acggcctttc cccacgcatc atactgtatc tcactgcggc tgccgtccgg ggcaacgata
     1201 gcggtgaggt ctcctgcggc gctgtactca taacgggttt cacgcccctg cgcatccctc
     1261 tgactgacca gttgaccgcg cgggttataa ctgctgtaag tgctgatgcc ttcttcccgg
     1321 tgaacggcga tttgttgacc gtaccggtca tactcatacc gcgtcgtgta ccccgagcag
     1381 tccgtaaacg cctgaagctg accgtaccgg tttcgcgtta ttctccgcct caccccggtt
     1441 ccgtcttcca ccgcacaggg caggccactg cgggaagaat cataaaacca gcgcgtaatg
     1501 ttaccgttgc gcgagg
//
LOCUS       DAETQN010000323         1480 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.332, whole genome shotgun sequence.
ACCESSION   DAETQN010000323 DAETQN010000000
VERSION     DAETQN010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.332"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="K0W68_004680"
     CDS             complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="K0W68_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HBI3075974.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHAAIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1090..1437)
                     /locus_tag="K0W68_004681"
     CDS             complement(1090..1437)
                     /locus_tag="K0W68_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HBI3075975.1"
                     /translation="MTALIILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDL
                     IRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQ
                     SHSMDNHTASNAH"
BASE COUNT          413 a          324 c          370 g          373 t
ORIGIN      
        1 gtgtaaggca cccacgcgga gtcaaagtgg atggatttca catccagtgt tttcttgatg
       61 aagtcggtgt tgtacagcag accatcatag gtagagttgg taattacagc atgtaccggc
      121 caggttgcgt ttggtgtttc tttcacgcgc ttagcaatgg cagcgtgctg gaattcactc
      181 tgtgggatac caccaagaat accgtaagcg ttacgggtcg ggcggaaata gattggcgta
      241 acatcgctca tcatcatcag gtgggtcagc gatttgtggc agttacggtc aatcagaatg
      301 gtgctgcctg ccggagcaga gtacatgcca acaattttgt tcgcagtgga agtaccgttg
      361 gtcaccatgt agctgcggtc tgcgttaaag acgcgagcga tatactgttc tgcttctttg
      421 tgtggaccac tatgatccag cagagaaccc agttcagata ctgaaatgga aatatcagat
      481 ttcatggtat tcggaccaaa gaaatcatag aacaggctac ctaccgggct tttctggaat
      541 gcagtaccgc ccatgtgacc aggagtacag aaagtatatt taccttcacg aacatattta
      601 aacagtgctt tagtcagcgg aggcagaata gtgttgatat attcgtcagt ggtctgcttg
      661 atcttattag caatatcttc agcagcaccc agcgcatatt caaagaagct aatctgtaaa
      721 cgcaggtcat tcaggcttac atcgagagtg gaatacgtat tagcgaacgc gtacaacggc
      781 aggttctcgt tcattttgct aatttcttcg cacagctcga gattatattt atcccagtca
      841 aaaataacgc cgcacagacg cgcattgttt tcgatcagtt ttaataagtc gtcacggtcg
      901 ttcgggtaaa caatctggaa gttcagacgt tcaagcgcgc gatgaagttc acggatgggt
      961 tcttctttaa aataaacccc catgtgattc aatattgcaa taacgttcat agtcatatct
     1021 ccaggtaaaa aaggcccctc ccaacacatg ggacaaaatg aaaggaggag cctcggaaaa
     1081 tacttttaat taatgtgcgt tagacgcggt gtggttatcc attgagtggc tctggcgctc
     1141 gtgcattttg cgagcgtaga acatcaggat aatcaggctg acgatgaagg tacctgccag
     1201 ctcgaaggag cttgcgccca tcagcgcgat gaagcagaac acacaaccca gtacagagca
     1261 gatcaggctg acaaagttgc ggatgttaac gccttcaaaa cgaatcaggt caacgcaaga
     1321 gtagaagtac ggcagcatag tcagcagtac tgcgataccg gtcagttcac cgaacaggtc
     1381 agatgcttta ccaccggcag agttcatcag agtgatgagg attatcaggg cagtcatttt
     1441 cactgcagcc agcagcagta atgccgatca gttaaggatc
//
LOCUS       DAETQN010000324         1462 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.333, whole genome shotgun sequence.
ACCESSION   DAETQN010000324 DAETQN010000000
VERSION     DAETQN010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.333"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            503..958
                     /gene="csgB"
                     /locus_tag="K0W68_004682"
     CDS             503..958
                     /gene="csgB"
                     /locus_tag="K0W68_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBI3075976.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            999..1451
                     /gene="csgA"
                     /locus_tag="K0W68_004683"
     CDS             999..1451
                     /gene="csgA"
                     /locus_tag="K0W68_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBI3075977.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          447 a          276 c          337 g          402 t
ORIGIN      
        1 aaatgataga aaagttgtac atttggtttt tattgcacaa ttttaaaaaa tcatacaaat
       61 ggtgataact tactaataat gcatataaaa aatatttcgg tgtagtcctt tcgtcatgta
      121 aaaagttgtt gttttttctc cacacctccg tggacaaatt tttactccaa aaagacgagg
      181 tttgtcacgg cttgtgcgca agacatatcg cagcaatcag cgatgggcaa gaagaatgac
      241 tgtctggtga tttttgatag cggaaacgga gatttaaaag aaaacaaaat attttttgcg
      301 tagataacag cgtatttacg tggcttttaa tactttggta tgaacaaaaa aagaaaaata
      361 caacgcgcgg gtgagttatt aaaaatattt ccgcagacat actttccatc gtaacgcagc
      421 gttaacaaaa tacaggttgc gttaacaacc aagttgaaat gatttaattt cttaaatgta
      481 cgaccaggtc cagggtgaca acatgaaaaa caaattgtta tttatgatgt taacaatact
      541 gggtgcgcct gggattgcag ccgcagcagg ttatgattta gctaattcag aatataactt
      601 cgcggtaaat gaattgagta agtcttcatt taatcaggca gccataattg gtcaagctgg
      661 gactaataat agtactcagt tacggcaggg aggctcaaaa cttttggcgg ttgttgcgca
      721 agaaggtagt agcaaccggg caaagattga ccagacagga gattataacc ttgcatatat
      781 tgatcaggcg ggcagtgcca acgatgccag tatttcgcaa ggtgcttatg gtaatactgc
      841 gatgattatc cagaaaggtt ctggtaataa agcaaatatt acacagtatg gtactcaaaa
      901 aacggcaatt gtagtgcaga gacagtcgca aatggctatt cgcgtgacac aacgttaatt
      961 tccattcgac ttttaaatca atccgatggg ggttttacat gaaactttta aaagtagcag
     1021 caattgcagc aatcgtattc tccggtagcg ctctggcagg tgttgttcct cagtacggcg
     1081 gcggtaacca cggtggtggc ggtaataata gcggcccaaa ttctgagctg aacatttacc
     1141 agtacggtgg cggtaactct gcacttgttc tgcaaactga tgcccgtaac tctgacttga
     1201 ctattaccca gcatggcggc ggtaatggtg cagatgttgg tcagggctcg gatgacagct
     1261 caatcgatct gacccaacgt ggcttcggta acagcgctac tcttgatcag tggaacggca
     1321 aaaattctga aatgacggtt aaacagttcg gtggtggcaa cggtgctgca gttgaccaga
     1381 ctgcatctaa ctcctccgtc aacgtgactc aggttggctt tggtaacaac gcgaccgctc
     1441 atcagtacta atacatcatt tg
//
LOCUS       DAETQN010000325         1431 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.334, whole genome shotgun sequence.
ACCESSION   DAETQN010000325 DAETQN010000000
VERSION     DAETQN010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.334"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            14..439
                     /locus_tag="K0W68_004684"
     CDS             14..439
                     /locus_tag="K0W68_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBI3075978.1"
                     /translation="MLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVK
                     TRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNM
                     IRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            464..>1431
                     /locus_tag="K0W68_004685"
     CDS             464..>1431
                     /locus_tag="K0W68_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI3075979.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDL"
BASE COUNT          386 a          331 c          341 g          373 t
ORIGIN      
        1 ttcgcaagaa aaaatattac gcattattca caccaaaggt gtgtcattgc ctgatgatcg
       61 cgagtcagtc ttctggctga atattaaaaa tattccgcca tcagcgagca acaaagcgac
      121 gaactcgctg gaaatagcag taaaaacgcg tatcaagctg ttctggcgac cagcaaatat
      181 ccgccttatt cccgaagatg ctgcgccgaa agtgaaatgg cgccgggaag ggcgaaatct
      241 gatagcagag aatcccaacc caattcatat cagtgtgatg gatgtcatcg ttgatggcca
      301 tgatgtgccc ctcaatatga tccgtccttt tgaaacgctc acgttacctt tacctgctaa
      361 ttctgcggga ggccaaatga cctggcgatt tattaatgat tacggcgcag tcagtgaccc
      421 gataaaaatg acgctgtaag caagggataa ggataggcgt attatgatga cgaccagaat
      481 agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga tggccgaggt
      541 cagtgtattc aatccggcgc ttctggaaat cgaccatcaa tccggagtcg atattcgcca
      601 gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt ttatcaacgg
      661 taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg ctgatctgca
      721 cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag ttgatgcgct
      781 caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac gtattgaagg
      841 ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc cccaaatcta
      901 catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg ggattaacgc
      961 gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt atggttcaca
     1021 agagacagat accagttatc tcaatctgcg caatggactg aatattggac cgtggcggct
     1081 acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact ccattagtac
     1141 ctggatacag cgcgatattg ccgcgttaag aagccagatt atgattggtg atacgtggac
     1201 ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata ctgataacga
     1261 tatgctaccc gccagccaga atggctttgc tcctgtggtt cgtgggattg caaagtccaa
     1321 cgccaccgtc atcattcggc agaatggcta cgtgatttat cagtctgccg ttccacaagg
     1381 tgcttttgag atcaccgatc tcaacaccgc cagtacaggt ggcgatttgg a
//
LOCUS       DAETQN010000326         1420 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.335, whole genome shotgun sequence.
ACCESSION   DAETQN010000326 DAETQN010000000
VERSION     DAETQN010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.335"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..683
                     /locus_tag="K0W68_004686"
                     /pseudo
     CDS             1..683
                     /locus_tag="K0W68_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            848..>1420
                     /locus_tag="K0W68_004687"
     CDS             848..>1420
                     /locus_tag="K0W68_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI3075980.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLICLCY
                     SQISLAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPMADFSS
                     VSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPRLNKLV
                     TEVIPSAVTSEGTKANAYKYTERYTAFYRVG"
BASE COUNT          373 a          320 c          360 g          367 t
ORIGIN      
        1 aagtctggcg tcagttgtta cgggaaggta tcagagtggc cagatgcact gtggcacgtc
       61 tcatggcggt tatgggactt gccggtgttc tccggggtaa aaaggtccgt acgaccatca
      121 gccggaaagc cgttgccgca ggcgaccgcg taaaccgtca gttcgtggca gaacgacctg
      181 accagctgtg ggtggctgat tttacttacg tcagcacatg gcagggcttc gtctatgtgg
      241 cgttcatcat tgatgtgttt gccggataca tcgtgtggtg acgagtctca tcgtctatgg
      301 aaacgacatt cgtgctggat gcactggagc aggcgttgtg ggcccgtcgc ccgtctggca
      361 ccatccatca cagcgataaa ggctctcagt atgtgtcact ggcctataag gcgcgactaa
      421 aagaagcaaa actgctggca tcaacaggga gtactggtga ctcgtatgac aacgcgatgg
      481 cagagatcat caagggcctt tacaaagcgg aggtaataca ccgtaagagc tggaaaaacc
      541 ggacagaagt ggagctggcc acactcacgt gggtggactg gtataacaat cgacgattgc
      601 tggaaaggct gggccatatc ccaccggcag aagcagaaaa agcttattat gcttccatcg
      661 gaaatgatga tctggcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      721 gttcagttca caaaaacaca ttaatgcagt aactatattt tcctttctgg tgataacgtc
      781 gggttatcat tagcttcttc agctatttta cttttatatc ccttgtaaac atcatggaga
      841 atccatagtg aataaagttt attctcttaa atattgcccc gtcaccgggg ggcttattgc
      901 tgtctctgaa cttgcccgca gggtaataaa aaagacatgc cgaagattaa cgcatattct
      961 tctaatctgt ctgtgttact ctcagatatc cctggcgggt attgtccgtt ccgatattgc
     1021 ctatcagatt tatcgtgatt tcgccgaaaa caaagggctt tttgtacctg gtgccaatga
     1081 tattccggta tatgataagg acggaaaact tgtgggaaga ctgggtaaag ccccaatggc
     1141 cgatttcagc agtgtgagct caaatggcgt tgctacgctt gtatcgcctc agtatatcgt
     1201 cagcgtaaag cataacggag gatatcggag tgtgagcttt ggtaatggga aaaatacata
     1261 ttcccttgtt gaccgtaata accacccttc tattgacttc catgctccac gtctgaataa
     1321 actggttaca gaagttattc cctcagcggt aacatcagaa ggaaccaaag ccaatgctta
     1381 taaatacact gaacgttaca ccgcttttta tcgggtgggt
//
LOCUS       DAETQN010000327         1420 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.336, whole genome shotgun sequence.
ACCESSION   DAETQN010000327 DAETQN010000000
VERSION     DAETQN010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.336"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>1420
                     /gene="rhsC"
                     /locus_tag="K0W68_004688"
     CDS             <1..>1420
                     /gene="rhsC"
                     /locus_tag="K0W68_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HBI3075981.1"
                     /translation="VLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRL
                     SAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAH
                     RHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGL
                     KRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASA
                     FYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATED
                     ATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQL
                     IAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDA
                     AGRVIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHW
                     HYDEADRLTHRTVNGETAERWQ"
BASE COUNT          338 a          421 c          436 g          225 t
ORIGIN      
        1 tggtactgac cacgcaggcg cagcgggcag aagaagcccg gcagcaggcc atttccggcg
       61 ggacggaacc gtccgctttt cctgataccc tgccgggtta caccgaatat ggccgggaca
      121 acggcatccg tctgtctgcc gtgtggctga cgcacgaccc ggaatacccg gagaatttac
      181 ctgccgcgcc gctggtgcgc tatggctgga cgccgcgcgg cgaactggcg gcagtgtatg
      241 accgcagcaa tacacaggtg cgcagcttta cttacgatga taaataccgg ggccggatgg
      301 tggcgcaccg tcacacgggc cggccggaaa tccgttaccg ttacgacagc gacgggcggg
      361 tgacagaaca gctaaacccg gcaggcttaa gctacacgta tcagtatgag aaggaccgca
      421 tcaccatcac cgacagcctg aaccgccgtg aagtgctgca cacgcagggt gaagccgggc
      481 tgaagcgggt ggtgaaaaag gaacacgcgg acggcagcgt cacgcagagt cagtttgatg
      541 cggtgggcag gctcagggca cagacggatg ccgcaggccg gacgactgag tacagcccgg
      601 atgtggtgac gggactcatc acgcgcatca ccacgccgga tggcagggca tcggcgtttt
      661 actataacca ccacagccag ttaacgtcag ccaccgggcc tgacgggctg gaaatgcgcc
      721 gcaaatatga tgaatccggc cgtctgattc aggaaactgc ccctgacggc gatatcatcc
      781 gctaccgtta tgataatcca cacagtgact taccctgcgc aacggaagat gccaccggca
      841 gccggaaaac catgacgtgg agccgttacg gtcagttgct gagcttcacc gactgttccg
      901 gttatgtaac ccgttatgac catgaccgct tcgggcagat gacggcggtg caccgcgagg
      961 aagggctgag tcagtaccgc gcatacgaca gccgtggaca gttaattgcc gtgaaagaca
     1021 cgcagggcca tgaaacgcgg tatgaataca acgccgccgg tgacctgacc accgtcattg
     1081 ccccggacgg cagcagaaac gggacacagt acgatgcgtg gggaaaagct atctgtacca
     1141 cgcagggcgg tctgacgcgc agtatggaat acgatgctgc cggacgggtc atccgcctga
     1201 ccagtgaaaa cggcagccac accaccttcc gttacgatgt actcgacctg ctgatacagg
     1261 aaaccggctt tgacggccgc acacagcgtt atcaccacga cctgaccggc aaacttatcc
     1321 gcagcgagga tgaggggctg gtcacccact ggcactatga cgaagcagac cgcctcacgc
     1381 accgcaccgt gaatggcgaa accgcagagc ggtggcagta
//
LOCUS       DAETQN010000328         1394 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.337, whole genome shotgun sequence.
ACCESSION   DAETQN010000328 DAETQN010000000
VERSION     DAETQN010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.337"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..863)
                     /locus_tag="K0W68_004689"
     CDS             complement(<1..863)
                     /locus_tag="K0W68_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBI3075982.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIW
                     HAFEHEEHANT"
     gene            complement(1179..>1394)
                     /locus_tag="K0W68_004690"
     CDS             complement(1179..>1394)
                     /locus_tag="K0W68_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBI3075983.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          389 a          247 c          309 g          449 t
ORIGIN      
        1 aaggtgttgg cgtgctcttc atgttcaaaa gcatgccata tctgtgatac atcagattgt
       61 ttgttttccg ggaaccatgc tgtcacggca tcagccaggg ggcgatggag tgtattctgt
      121 tgtccgtcac tcatggagaa gtaaatccgt gggccgtggt agtccggttg ggcggtttgt
      181 tgtaacaaag tttgcattat tctgggagat agttgatttt ctctaagatt gacaacgcta
      241 tcagaactga ggccaagtat gctttccgga attgccgtga tccggttctg attaagccag
      301 aatattctta taatatgatc atctctttct ggaaaatctg gtattacttc caaagcattt
      361 ctggctgcat caagtaattc caatgacatt ggtaacctcg gaagtacaga caggtgattg
      421 tcacttacat taatatattc tagtgacgca ggtaattcag gaagtgcgaa caaatggtta
      481 tcactcacat ttatatattc cagcgacact ggtaattcag gaagtgcaga taattgatta
      541 ctgcttgcat tcagctcttt caatgccctt ggtagctcgg ggagcattga tagttggtta
      601 ttgcttacat taatttcatc aagattgtca ggcaatcgtg gtagagatct gagacctaaa
      661 caagataagt ccaacgatgt ttcactgttc tccagacata attggagccg ggtaaaagca
      721 gtttccctgt tttctccgga aatgctgttt ttagtccatt caacccattc ggagagataa
      781 ttattgtgaa cattgtcgat tgatgtagtt ctgtaaaaag agacgtttcc agtggatagg
      841 gggggattat ttacaggaag cataataacc tcgcagaagg atattctgat aaaatgtgat
      901 actgggaaat aataagctga aataaaatgt ttcaggattg aaatttattg ttttcatttt
      961 aaggaagaga tgttgattta actgtaaaaa aacgatacta ttttcttaga tggtaaatgt
     1021 ctcgcctgtg ctatattttg tttttcggtt agttaaatat cgtacgttca tatttgaacg
     1081 ttctgccgga atgcattatc aatagaggta aagtcgcaac cccaaatcgt aaaggaaacc
     1141 gtagcacgtc ttaagcaaga acgtgctgcg gttggatgct attttttccc tgaagcggaa
     1201 aacattacta cagtaccttg aaccttggtt ttaacattct cgaaatgctc tgagagtata
     1261 tgtgttaagc cttcttcgga atcttttgtg tttgaaaaga tgcctttctg attgtaaatg
     1321 cgcatcagtt tttgaccgaa gctattgtgc acaactccat cgccaagaat tgtggctccg
     1381 tatagagttc catc
//
LOCUS       DAETQN010000329         1392 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.338, whole genome shotgun sequence.
ACCESSION   DAETQN010000329 DAETQN010000000
VERSION     DAETQN010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.338"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..459
                     /locus_tag="K0W68_004691"
     CDS             <1..459
                     /locus_tag="K0W68_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI3075984.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="K0W68_004692"
     CDS             477..1115
                     /locus_tag="K0W68_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI3075985.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          307 g          359 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac ac
//
LOCUS       DAETQN010000330         1376 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.339, whole genome shotgun sequence.
ACCESSION   DAETQN010000330 DAETQN010000000
VERSION     DAETQN010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.339"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            3..983
                     /locus_tag="K0W68_004693"
     CDS             3..983
                     /locus_tag="K0W68_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBI3075986.1"
                     /translation="MVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            998..>1376
                     /locus_tag="K0W68_004694"
     CDS             998..>1376
                     /locus_tag="K0W68_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBI3075987.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          359 a          315 c          363 g          339 t
ORIGIN      
        1 tagtggtggg tgttccggtt ccggacaata ttcacccgat gatcaaagac gaagcaggag
       61 tctggtcgtg gcgcacgcct gtcctgaaag gcaacctgta tgagtatttt ttcaatgttg
      121 atggtgtacg cagcattgat ataggcaccg caatgactaa gcctcagcgc caggttaact
      181 ccagtatggt tctggtgcca ggcagttatc tggatacgcg ttctgttgcg catggtgatt
      241 tgatcgccat aacttaccac tccaacgcat tgcaatctga acgtcagatg tatgtctgga
      301 ccccgccagg atacaccggc atgggcgagc ctttgccagt gctctatttc tatcacggtt
      361 ttggtgatac cggacgttcc gctatcgatc aggggcgtat cccgcaaatc atggataacc
      421 tgctggctga agggaaaatt aaaccgatgc tggtggtgat cccggatacc gaaaccgatg
      481 cgaagggcat tattcccgaa gatttcgtgc ctcaggaaag acgtaaagtc ttttatccgc
      541 tgaatgctaa agcggcagat cgcgaactga tgaacgatat tatcccgctg attagcaagc
      601 gttttaatgt ccgtaaagat gccgatggtc gcgcgctggc agggctttca caaggcgggt
      661 accaggcgct ggtttccgga atgaatcatc tggaaagctt tggctggctg gccacattca
      721 gtggtgttac cacgacaacc gtaccggatg aaggtgtcgc ggcccggctg aacgatccgg
      781 cagctattaa ccagcaacta cgtaatttta ctgtggtcgt tggtgataaa gatgtcgtaa
      841 ccggcaagga tatcgccggg ctgaaaactg agcttgagca gaaaaaaatt aagtttgatt
      901 accaggaata tcccggcctg aaccatgaaa tggatgtctg gcggcctgcc tatgcagcct
      961 tcgtacagaa attatttaaa taaaataagg cattaagatg aaattaatca ttaccgaaga
     1021 ttaccaggaa atgagccgtg tcgcggcaca ccatctgctt ggttatatgt cgaagacgcg
     1081 tcgtgttaac ctggcaatta ccgccggtag cacgcccaaa ggcatgtatg aatacctcac
     1141 caccctggtt aaaggtaagc cctggtacga taactgctat ttctataatt tcgatgaaat
     1201 tccctttcgc ggcaaagagg gagaaggcgt aacgattacc aatctgcgta atctgttttt
     1261 cacccctgcg gggatcaaag aagagaatat ccagaagctc actattgata actaccgcga
     1321 gcatgatcag aaactggcgc gtgaaggcgg cctggatttg gtggtgctgg gattag
//
LOCUS       DAETQN010000331         1348 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.340, whole genome shotgun sequence.
ACCESSION   DAETQN010000331 DAETQN010000000
VERSION     DAETQN010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.340"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..478)
                     /gene="umuC"
                     /locus_tag="K0W68_004695"
     CDS             complement(<1..478)
                     /gene="umuC"
                     /locus_tag="K0W68_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBI3075988.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
     gene            complement(478..897)
                     /locus_tag="K0W68_004696"
     CDS             complement(478..897)
                     /locus_tag="K0W68_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBI3075989.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1270..>1348
                     /locus_tag="K0W68_004697"
     CDS             1270..>1348
                     /locus_tag="K0W68_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HBI3075990.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDL"
BASE COUNT          405 a          321 c          277 g          345 t
ORIGIN      
        1 tctgccgctg ccattttttt gccgcatgat tggcaagctt agccagcgtt ttggtctggg
       61 cgatccccat accaacagta agatgggtac gttgtagcac cgttgcgcga atttctctgc
      121 caaaatcagt cagatcgcga caattacgca cacctgtcag atcgcagaat gcctcatcaa
      181 tactgtaaat ctcgacgcgg ggcgatagtt cttccagcgt cgacatcacc cgattgctca
      241 tgtctgcgta aagctcataa ttgctgctaa agcaaaccac accacagcga cgaaacagat
      301 ctttttgttt gaaccaggga tcgcccattt taacacccag cgcctttgcc tcagcgtttc
      361 gggcgataac gcaaccgtca ttattcgata gcacaaccac cggtttaccc cataaatcag
      421 ggcgaaacac cgtctcacag ctggcataaa acgcgtttac atcacagaga gcaaacatca
      481 gcgcatcgcc ttaacgacgt ggatcaccac accaaagaca tccagcgtat cttcgctact
      541 gatggtaatg ggcgagtacg cactgttcat gggaataagc tgtaccgtcg ggcgtagttg
      601 caattttttc accgtaaact cgccgtcaac agcagcgatg acaatatcac catggctggc
      661 ggtaatagcg ctatcgacaa tcagtaaatc accgtcacta attccaccat caatcataga
      721 atcaccactt gctttgacga agtaagtcgc gctgggatgc tggatcaaca gttgattcag
      781 atcgatgcgc tgttcgacgt aatctgctgc cggtgaagga aagccacact gaacaagatc
      841 gctaaatagc ggaaaagtca caatttcgcg gagatccgca ggcttgataa acaacataat
      901 aatctgcctg aagttatact gtttttatat acagtaatct gttcttgcca gcagatcaat
      961 actgattcag gctatcaata tttgtcgctg catagactgc tgatttttcg ttctcttatc
     1021 ctgtgctcac gtggccttct ggcgacgacg ctcatccagc agaaatgaaa aatatcaccc
     1081 ggcaaaaaaa tagaatagaa gcattcgcca taatgacatt aaacattgtt tgatatttat
     1141 catattaata gaaataaaga cattgacgca tcccgcccgg ctaactatga attagatgaa
     1201 gtaaaattta ttaatagttg taaaacagga gtttcattac aatttatata tttaaagagg
     1261 cgaatgatta tgactgaaat cgttgcagat aaaacggtag aagtagttaa aaacgcaatc
     1321 gaaaccgcag atggagcatt agatcttt
//
LOCUS       DAETQN010000332         1341 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.341, whole genome shotgun sequence.
ACCESSION   DAETQN010000332 DAETQN010000000
VERSION     DAETQN010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.341"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..184
                     /locus_tag="K0W68_004698"
     CDS             <1..184
                     /locus_tag="K0W68_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075991.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="K0W68_004699"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="K0W68_004699"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBI3075992.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAETQN010000333         1300 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.342, whole genome shotgun sequence.
ACCESSION   DAETQN010000333 DAETQN010000000
VERSION     DAETQN010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.342"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            271..528
                     /locus_tag="K0W68_004700"
     CDS             271..528
                     /locus_tag="K0W68_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3075993.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            528..902
                     /locus_tag="K0W68_004701"
     CDS             528..902
                     /locus_tag="K0W68_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBI3075994.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            899..>1300
                     /locus_tag="K0W68_004702"
     CDS             899..>1300
                     /locus_tag="K0W68_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBI3075995.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          361 a          286 c          350 g          303 t
ORIGIN      
        1 gcgatctttt cctgtgtggt acgccgtctg cgtttctccg gccctaagac atcaatcatc
       61 tgtactccaa tgactagtct aaaaactagt attaagacta tcacttattt aagtgatact
      121 ggttgtctgg agattcaggg ggccagtcta cggacatttt ctgcctgtgg accaggaaag
      181 tgcacgggtg ctttccactc ccatgaagcc cggcaatccg cgcagataca gtaataaacg
      241 aactgacagc agtaagatgg agcgtttctt ttgaacaacc agacaatgac ttttacccct
      301 gaacaattgc gtaaacacgc gcaggaaatg ctgcgtcatg ctgaacaact cgaaaaaacg
      361 ggcataacaa aagacgctat ccgtaaagat atggtacccg cgcttcggga actgatgcag
      421 gcgaagcatc gcgcacagaa agcagtagat gagctggtgg actgtgtggc agagctggaa
      481 accagagtcg gaaagtttga aaaactggtg caggaggtgc tgcgctgatg cgccatgagt
      541 ttattttacc ttatccgccg acggtgaata cttactggcg acgtcgtgac aacacatatt
      601 ttgtatcaaa agccggtgag cgttatcgcc gggatgtggc gcttattgtc cgtcagcagc
      661 gactgaaatt aagcctgtcc ggaagactgg cgataaagat tattgcagag ccaccggata
      721 agcgccgtcg tgacctggac aatatcctga aagcaccact ggatgcactg acacatgcgg
      781 gagtgttaat ggacgatgag cagtttgatg aaatcaatat cgttcgtggt cagccagtat
      841 ctggtggacg tctgggggtg aagatttacc ccataatgca tgaagagcag gtcaaaaaat
      901 gaaactggaa gatttaccga aatactactc cccaaaatcc ccttgcctga ccgatgcatc
      961 ggcctcaacg tcaaaagatg cgctgagtat cactgatgtg atggccgcgc agggcatgac
     1021 acagaatcgg gctgagatgg ggttttctgc gttcctgggg aaaatgggca tcagtatgaa
     1081 tgacagggcg cgggcaacag aattactggc agattatgca ctcagtcggt gcgatcgtgt
     1141 ggcggcgttg agaaaacttc cggcagaaat aaaaccggta gtgatgcgca ttatggcttc
     1201 gtacgctttt gaggattatg cccgcagcgc agcgagtaaa aagcagtgcc cttgttgcta
     1261 tggggaaaaa tttattgaaa gcgtagtttt tacaaacaag
//
LOCUS       DAETQN010000334         1294 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.343, whole genome shotgun sequence.
ACCESSION   DAETQN010000334 DAETQN010000000
VERSION     DAETQN010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.343"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..1112
                     /locus_tag="K0W68_004703"
                     /pseudo
     CDS             1..1112
                     /locus_tag="K0W68_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          355 a          262 c          399 g          278 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacacc actggggtta gaacgagtta
      661 taagtgtata tgggcctccc gctccagacc gggcaggtgc tgaaactcct cttgttctta
      721 ctgatatgac tggaggagtg actatttatt atgatcctga aacaggtgat tcaatgactt
      781 ttgatagtag caatagaatt gatcgtcgtt ctcaacgtgg tgctggtgat ccatatacag
      841 gtgaagtcgt tggttgtgag acaaatggat caggaatatc ggcagcatat ggcacaacga
      901 agatctatac tacagatact agagcaagat ggctacatgg tgggggatca tcgttgcgcg
      961 atccttatgc tcctcgacaa ggctggaaac caactatggg atgtacccgt gcacagaatg
     1021 aagatgtaga tgagttgtgt aagaaagtga caagttggat gtattcgcat cctggagaga
     1081 gaattagata tgaacgtttt aagacacgtt aatgcgatta acatgggagt gtgcattcta
     1141 attttggtta ttggatgtgc aaatgctaaa gttcttaaac gatgtatatt acccgctcct
     1201 gatggtggcg tagaaaaact atcaaaaccc aacttacatg gagtaattaa aaaagttgat
     1261 ttgaacacaa atgaagcgga aattaaaata aaag
//
LOCUS       DAETQN010000335         1265 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.344, whole genome shotgun sequence.
ACCESSION   DAETQN010000335 DAETQN010000000
VERSION     DAETQN010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.344"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(24..>1265)
                     /locus_tag="K0W68_004704"
     CDS             complement(24..>1265)
                     /locus_tag="K0W68_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3075996.1"
                     /translation="IKHIIIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAP
                     GWLELSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYPTINQRKVRIS
                     KTKKPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFY
                     AVQQLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNK
                     ESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTE
                     TLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEA
                     QIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDM
                     LIFGEPVTRIYPAGDMEEVDA"
BASE COUNT          333 a          298 c          228 g          406 t
ORIGIN      
        1 tcagtgaacc ctaacaaaaa atactaagcg tccacctctt ccatatcacc agcaggataa
       61 atccttgtta ctggttcacc aaaaataagc atatcatcta atgactgcat ctcatcggtt
      121 gcttcttcaa gcgttaaggt gtggtcatct ttaccatcaa gaggaaaacc acgaatcata
      181 tttccattgt gaagatatat atccaccagc ttttcattac cagaaacaac aatctctttc
      241 accagacgct taataataat ctgggcttca gttctcccct ctacagactc catatcaaga
      301 ccgcttaaat ccaagcttga tacagatttt accctcgcct tttctcgtga taactgaact
      361 atcagttcat ctttttcttc ctgtttaacc cgtagtgctt cttgaatctg agtgatgtta
      421 ggaagctgta tcagcgtctc agtgagtttt tccagttcac catcaacggt gtttaatctt
      481 ctttgtagtt catctaattt agctgtatcg gtagcttcat cgtgtagctg tgacagtttg
      541 ctaaacagac gactacaaag ctgtgtatca agtaactttc tggacactgt accgtagcta
      601 caacgggact ccttattacc attgcaacgg taagtacctt gataaacggg tttacgtatc
      661 cctgtaggag tcattacaag cccacactta caacggataa gccccttcag tatgttcaca
      721 tatagccagt gttcaccgct ggatgttttc cccttccctg tttcctctaa cagttgctgt
      781 acagcgtaaa acttagagtc tgtgacaatc tgaggataga acccttcaat ttcttcacgt
      841 aacactgttt tacggtcttc tgtagaaatc ttagcgggaa ggtaacctaa tacacttcgt
      901 gaagtcagga tgtgtttaac gcttaatgct gaccagttgc catccggttt cttagtcttg
      961 gatattctga ccttacgctg attaatggtc gggtaacctt gttcgtttag ccatttacag
     1021 atagcaaaca tcgacatacc agaaagacgc ttgtcataga tgatattcac cagtgacgct
     1081 ttatcttcat caacgatgta gtatgaacca tcttctgata gctctaacca tccgggggct
     1141 tgcttaccca taggcttctt aagttctctt gcaagctgtg ttttatggtt ccagacctga
     1201 gctatacgct tttgcttaac aagagattct tggtgtgctc tctcagctat gatgatgtgc
     1261 ttaat
//
LOCUS       DAETQN010000336         1232 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.345, whole genome shotgun sequence.
ACCESSION   DAETQN010000336 DAETQN010000000
VERSION     DAETQN010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.345"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            368..1156
                     /gene="virF"
                     /locus_tag="K0W68_004705"
     CDS             368..1156
                     /gene="virF"
                     /locus_tag="K0W68_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HBI3075997.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(1098..>1232)
                     /locus_tag="K0W68_004706"
     CDS             complement(1098..>1232)
                     /locus_tag="K0W68_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI3075998.1"
                     /translation="NENIVLLGPSGVGKTHLAIAMGYEAFKIFYDISKISLELYHNIH
                     "
BASE COUNT          432 a          193 c          205 g          402 t
ORIGIN      
        1 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
       61 ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag
      121 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      181 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      241 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      301 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
      361 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
      421 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
      481 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
      541 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
      601 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
      661 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
      721 caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag cttgcctttt
      781 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
      841 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
      901 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
      961 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1021 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1081 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1141 tcataaaaaa ttttaaatgc ttcatagccc atcgctattg ccagatgggt tttccccaca
     1201 cctgatggcc ccagtaatac gatattttca tt
//
LOCUS       DAETQN010000337         1213 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.346, whole genome shotgun sequence.
ACCESSION   DAETQN010000337 DAETQN010000000
VERSION     DAETQN010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.346"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            231..>1213
                     /gene="gspA"
                     /locus_tag="K0W68_004707"
     CDS             231..>1213
                     /gene="gspA"
                     /locus_tag="K0W68_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HBI3075999.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQDIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSG"
BASE COUNT          329 a          249 c          310 g          325 t
ORIGIN      
        1 agcatcttta ttatgattcg ccagatggaa ggggaaacgt agtgtgggca cgatgtatgt
       61 tctaataaaa tagattgggt gggttgtata ttgacgttag tataaatagt attaatgccg
      121 cttgcatgaa tcaagcaaaa tttatttata ttagtaatat gtatttaatt aataaataca
      181 tacattattt ttatgattaa ggataatcaa taattaaaac aggaagttct atgtctacga
      241 gaagagaagt tattctttcc tggttgcgtg agaaacgtca gacctggcgt ctgtgctatt
      301 tgttgggtga ggctggaagt ggaaaaacct ggctggcgca gcaactgcaa aaagataaac
      361 atcgccgtgt gattacttta agcctcgttg tttcctggca aggtaaggcc gcatggatcg
      421 ttaccgacga taacgcggct gaacagggct gccgtgacag cgcctggacg cgagatgaga
      481 tggcggggca attactgcat gcgcttcatc gaactgatag tcgctgccct cttattatta
      541 ttgaaaatgc tcacctgaat caccgccgca tacttgatga tctccaacgc gcgatatctc
      601 ttattcctga cggtcaattt ttgctgatcg gcagacccga tcgaaaagtc gaacgtgatt
      661 ttaaaaaaca ggacattgaa cttgtctcga caggacgtct gacggagcac gaacttaagg
      721 caagcatcct tgaaggtcag aatattgacc aacccgatct ccttttaacc tccagagttc
      781 tgaaacggat agctttatta tgtcggggcg atcgcagaaa gctggcgctt gccggcgaaa
      841 caattcgctt attacagcaa gccgagcaga ccagcgtttt cactgcaaaa caatggcgaa
      901 tgatttaccg catattgggt gataaccgcc cccggaaaat gcagcttgcc gtggtaatga
      961 gtggaaccat tatcgctatt acctgtggtt ggctactgct gtcctctttc acagctccgt
     1021 tccccgttcc tgcgtggctg gtacccgtaa cgccagtagt taaacaagat atgactaagg
     1081 atattgctca tgtggtgatg cgcgatagtg aggcgctaag cgtgttatac ggcgtatggg
     1141 ggtacgaggt cccagctgat tcggcctggt gcgatcaggc agtaagagcg gggctggcat
     1201 gtaaaagtgg gaa
//
LOCUS       DAETQN010000338         1211 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.347, whole genome shotgun sequence.
ACCESSION   DAETQN010000338 DAETQN010000000
VERSION     DAETQN010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.347"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            54..1211
                     /locus_tag="K0W68_004708"
                     /pseudo
     CDS             54..1211
                     /locus_tag="K0W68_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT          321 a          305 c          273 g          312 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
      841 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
      901 tttacgccgg acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt
      961 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
     1021 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
     1081 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
     1141 aagttttact caataacgat tactggtgtc gggcattatc atttagtcct acgaaagcga
     1201 agaactatca g
//
LOCUS       DAETQN010000339         1200 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.348, whole genome shotgun sequence.
ACCESSION   DAETQN010000339 DAETQN010000000
VERSION     DAETQN010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.348"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            223..1038
                     /locus_tag="K0W68_004709"
     CDS             223..1038
                     /locus_tag="K0W68_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3076000.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          365 a          230 c          234 g          371 t
ORIGIN      
        1 tggtttaaca tattaacttc gctctccact taacttttta gttaaggaga gcgaaataat
       61 atcaaagtag cagtaaaacc tataacgtaa attcaaattg ttaaattaac tccctccagt
      121 acacaatact tctcacgtta gttatgagcg atttctgata gtgcctggtt taatcagcgc
      181 tttattatct gcgacgttta tttttattta agagagatcg cgatgatatc attaaaagct
      241 ccgcacaata atttgatgcc atatacgcaa caaagcatac ttaatacggt taaaaacaat
      301 cagttaccag aggatatcaa aagctcccta gtttcctgtg tcgatatatt caaggttttg
      361 attaaacaat attatgatta tccttatgat tgtcgtgatg atttagtcga tgacgacaaa
      421 ctcattcatc tcatggctgc cgtacgagac tgtgagtggt cagacgataa cgcactcacc
      481 ataaatgtgc agtttaatga tttcccagga ttttatgact ggatggatta ccctgatcac
      541 ccggttaagt ttgtttttcg catacttgaa aatcaaaaag ggacagtttg ggtatatgac
      601 caggatgatg cgtttcttga tattaaagca aatgtgcagg ctggacgctt taccgggctt
      661 aagaaattag tgcagtttat caattcagtg cgaacagatt gcaaatgtat tcttcttgaa
      721 caccatatgc ctttgctgag acttttccct aaagggaaag agtgtatgca tgtggaaaaa
      781 tggcttcgcg aaatgtcgtc tattccggag acagacgcgc ccattaagca ggcgcttgcg
      841 catggattat tattacactt aaaaaatatc taccctgttt ttcctgaatc actggtgatg
      901 ttattgctct ctgttctgga cgtaaaaaca tacagagatg atgcacgatt aaatgaatgg
      961 atatctaata gagtgcagga attaggtgac agatattatc ctgtaaataa gcacgttaaa
     1021 atccgctaca ctctttagtt cagaaagggt tccacctgca agttgttatt ccaccagaga
     1081 gtgaatcgca aagcaggtaa tcatttcatc tgtatatccg tatgccaaat ggcattccac
     1141 cgccttttcg ccgatatttt gttgtgatcg gcgacacttc gcttaaaaaa gcaccagcag
//
LOCUS       DAETQN010000340         1199 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.349, whole genome shotgun sequence.
ACCESSION   DAETQN010000340 DAETQN010000000
VERSION     DAETQN010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.349"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="K0W68_004710"
     CDS             complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="K0W68_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HBI3076001.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNV"
BASE COUNT          341 a          215 c          259 g          384 t
ORIGIN      
        1 ttaacattcg tacaaatggg caatgtgatt atattttcgg gtaatgaagt tagtaggtta
       61 ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg ataatctgcg tagttgattt
      121 tcacctacct tcaaatattt taagcgctga ggaaattctg gtagttcctg tagtctgttg
      181 ccggaaacaa agagttcttg tagtctatga ggcaattcag gcaagacagt aaggcgatta
      241 ccttcaatac gtaatattgt tagcgttgat ggaagctctg gtaacgtctc cagactatta
      301 aaacgtgccg agagtgattg taaatatggt ggtaatgatg gcagacatga caaatttcga
      361 ttgaatgaaa tattaagctt aatcagttga gatggaagtg tcgggagtat attcaagttg
      421 ttaccattta catgaagttc tgtcaaaagc ggagaatttt caggtaggga gattaactca
      481 ttgttggaaa tattaagttc acgtatatgc aaagggagtg gtggtaaaga acgtagtttc
      541 agtaagctta aattgagaac aggctcccgg gtatccagac atattttcaa taatcgaacc
      601 gcctggatgc gattttctgt tgttgttcct tccctagccc agtcatccca gatgcggtgg
      661 taatctctgg tacactgttc ttctaccgat aaacgagata tacatgataa gccacaaagg
      721 gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt aacccctcac tattcatatc
      781 ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa aaacaatcct agagctttat
      841 cggcgttttc attccagctg gaggaatatg ttcctgtctt tccagtaacc gtcgattgtt
      901 atatcagtct acccatgtta atgtgaccag ttctactttc atccagtttg gattgatctt
      961 tatcccctat attttcaaac aatttttatc atttaactca ttactgtctc gatattccca
     1021 tagcaaacga tctggttggt tagcccctca aaaaagtcag ttgcacgccg taaatccctg
     1081 ccggtattga gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt cacagttttt
     1141 ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt gattatgaat ggtgcagtc
//
LOCUS       DAETQN010000341         1192 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.350, whole genome shotgun sequence.
ACCESSION   DAETQN010000341 DAETQN010000000
VERSION     DAETQN010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.350"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..297)
                     /locus_tag="K0W68_004711"
     CDS             complement(<1..297)
                     /locus_tag="K0W68_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBI3076002.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPS"
     gene            complement(345..917)
                     /locus_tag="K0W68_004712"
     CDS             complement(345..917)
                     /locus_tag="K0W68_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HBI3076003.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          380 a          247 c          221 g          344 t
ORIGIN      
        1 cgaaggttct aatcgtgata ctggcggaat aacggtaaac ggcgctttgc ttttatcctc
       61 tttattaaat ggatctaccc aagcctggat gagataaggt attttttctt ctttgctaaa
      121 tacagaaata gatttatcag cattattact taagtaaacc acccgcgtac caccaatgac
      181 aacgcctgca taagttaatg ctggttggcc taaaaaaacg ctgagtataa tcgcggtaaa
      241 taacttacat gaaactgttg ttttaaagag cctgattaaa caactttttc tggacataac
      301 gactccctgg ccaaagccaa ctgaataaaa aggcccttga taaattattt cttatattcg
      361 acagtaaatt gtgaatcagc gttcgccgta ccagccgtta cggtcgcatt ggttgccaca
      421 tagcgtgcaa taaacttcat ttcagccttg ccttcagcat cgatagcggt ataagcagag
      481 tcgttataaa gtttaacctg cgaattatcg gactggttaa aaatacgaat accaacgccg
      541 gttgccgcaa tcgcttgtcc tgtaccggta aaatcaccag ggttgcctgc tgtaagcggc
      601 gtacccactt tcaggtcgcc gtcgccgcca ggcgcttctg taccatcaaa acgaacggct
      661 gcttgagtgt acgttgccgg gcaattttca agtttaatac tgaacttctg tggcgacgcc
      721 agactaccta cacctgaaaa tgcattttta ctgactttac ccagtgggac aatttgatcg
      781 gctgtatcgg aggtaacttc acaagtagtg tcgacaattt caccgacgaa gtgaacaaca
      841 ccatcttctg ccagtgcatg cccggccagc attagagatg ccagagtgaa tgcaccgcct
      901 ataatattac gcttcataaa aactccattg atataatata tcctttacca tgaatcatca
      961 cttatattaa taagtgtatt tttgattcaa aggaatagta caccaaagag acggctttat
     1021 ctaaaaataa tattaattac gcaaaaataa aatataaatt cattgtttaa atattattta
     1081 aacagtaaaa aatcaagttt gttatcagac attaatgata attcagaata tatttatata
     1141 gaaagataga taaatttatt aatgtcagtt ataaaaaagg gccaccggtc gg
//
LOCUS       DAETQN010000342         1190 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.351, whole genome shotgun sequence.
ACCESSION   DAETQN010000342 DAETQN010000000
VERSION     DAETQN010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.351"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>1190
                     /locus_tag="K0W68_004713"
     CDS             <1..>1190
                     /locus_tag="K0W68_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBI3076004.1"
                     /translation="AEQEALIHALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVS
                     RRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAP
                     CCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERG
                     IAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEAL
                     HGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDR
                     KGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALT
                     EEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELA
                     KAFA"
BASE COUNT          265 a          339 c          375 g          211 t
ORIGIN      
        1 gccgaacagg aagcgctgat ccacgccctg caggaaaagc ttagcaaccg ggagcgcgaa
       61 atagaccatc tgcaggcgca gctggataaa ctccgccgga tgaacttcgg cagtcgttcc
      121 gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc tgaaccggct tcagaaagag
      181 agcgatacgc tgactggtag ggtgtatgac ccggcagtac agcgtccgtt gcgtcagacc
      241 cgcacccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact gttgcctgcg
      301 gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga taccgccgaa
      361 cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga aaaacatgcc
      421 tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat cgagcggggt
      481 atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga gcacaccccg
      541 ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg ttcactgctg
      601 tcgggctggg tggatgcatg ctgccggctg ctgtctccga tggaagaggc gcttcatggc
      661 tatgtgctga ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt gctgttgcca
      721 ggcaataaga aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga ccgtaacgcc
      781 gggtcaacgc tggcgccggc ggtgtggttc gcttacagcc cggacagaaa aggcatccat
      841 ccgcagaccc atcttgcggg gttcagtggt gtactgcagg cggatgcata cgccgggttc
      901 aacgagctgt accgggatgg ccggataacg gaagccgcct gttgggctca cgcccgccgt
      961 aaaatccacg atgtgcacgt tcgcaccccg tcagccctga cggaggaagc gctgaaacgg
     1021 atcggcgaac tgtacgccat cgaggcagag ataaggggaa tgacggcgga acagcgcctt
     1081 gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg gctgcgtgaa
     1141 aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata
//
LOCUS       DAETQN010000343         1179 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.352, whole genome shotgun sequence.
ACCESSION   DAETQN010000343 DAETQN010000000
VERSION     DAETQN010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.352"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1000
                     /gene="htrE"
                     /locus_tag="K0W68_004714"
     CDS             <1..1000
                     /gene="htrE"
                     /locus_tag="K0W68_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HBI3076005.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIILNGIRCQIQ"
     gene            1017..1121
                     /locus_tag="K0W68_004715"
                     /pseudo
     CDS             1017..1121
                     /locus_tag="K0W68_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          356 a          236 c          276 g          311 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 atctattatt ctgaatggaa ttcggtgtca gattcagtaa ctacaaggaa cgtcaaatga
     1021 taaaaataac gccacataaa atagcgatac tgatgggaat attattatca ccatcagtat
     1081 ttgcaacgga tattaatgta gagtttacag ccactgtcaa agtagactgg ccccctgaat
     1141 ctccagacaa ccagtatcac ttaaataagt gatagtctt
//
LOCUS       DAETQN010000344         1160 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.353, whole genome shotgun sequence.
ACCESSION   DAETQN010000344 DAETQN010000000
VERSION     DAETQN010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.353"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            247..492
                     /locus_tag="K0W68_004716"
     CDS             247..492
                     /locus_tag="K0W68_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3076006.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(509..1141)
                     /locus_tag="K0W68_004717"
     CDS             complement(509..1141)
                     /locus_tag="K0W68_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBI3076007.1"
                     /translation="MDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRRAGVLTG
                     LGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQSTPQMST
                     LQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWAGIVLAS
                     VIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          351 a          277 c          275 g          257 t
ORIGIN      
        1 tttcgcagcc atgcggtgta taaaaaatga tctcatgcag atgttttgtg aatgtgtggg
       61 ttgacattca tatgaaaaaa atcataattc catcatgttt gtgtggggtc ttttctgtac
      121 cttacgcatc gcactcaagc ctgacagaaa atatgctgta aggctcatat caaaaaccgc
      181 cattagctca tcaggaagag cagacgacaa ccaggattgt tgtatggtac gggggtcgag
      241 gcctcgatgg cggtccaact tttcaaaact cttctgaatc agatcccctt attatcatcc
      301 ctgcaaagtg gaacattgcc attgtttggc tattcaggac gggtcagacc aatgaaaaaa
      361 gcccacacag gggagagtgg gctgaaatgg gaagctaaag actcaagtaa acttatcgga
      421 aataaggacc acgcattacg ggggctatca tcgccgatag tcgggattcg gcaaatcagg
      481 ttgattacgt agagagtaaa ttatctgctc accgctgcgt caccccttcg taaatcaggc
      541 gtagcgcgaa tacaccaata attgcaccaa taacccgact ggcaacgcgt tgcatacgcc
      601 cataagcacg acgcacagcg ggcaaagaaa acgcctgact aagaaagact cgccagataa
      661 ctgatgcgag cacaatcccc gcccaggcca ttaaacgtgc ccatgttggt gtttcggcat
      721 ttaatgttac tgagaaaata ctgataaaaa ataaaacggt ttgcgggtta gagagatcgg
      781 taattaatcc gcggcgaaaa aagacatacc aggaggcgct aatcggttgt tgtagtgtgc
      841 tcatttgcgg tgttgactgg cggcgcatgc tgcaccacgc aaaccataag agataagcgc
      901 cgccgacgat tctgataagc gaaaaaatct cctcacactg cgttattagc gttgcaagac
      961 caaacaaacc caacccggaa taaaatgcat cgcccagcgc cacgcccagt ccggtcagca
     1021 cccctgcgcg tcgaccggaa gccaggctgg tttgtactac cacaaagaga ttggctcccg
     1081 gattaaaaaa agtaatcacg aacagtccta cggtcaggta aacggcgtgc aaaggatcca
     1141 tagtgatttc atccataaat
//
LOCUS       DAETQN010000345         1104 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.354, whole genome shotgun sequence.
ACCESSION   DAETQN010000345 DAETQN010000000
VERSION     DAETQN010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.354"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            122..589
                     /locus_tag="K0W68_004718"
     CDS             122..589
                     /locus_tag="K0W68_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3076008.1"
                     /translation="MSVKLRLPQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVGLI
                     LHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFVHK
                     IKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
     gene            741..1003
                     /locus_tag="K0W68_004719"
                     /pseudo
     CDS             741..1003
                     /locus_tag="K0W68_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904911.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          328 a          228 c          243 g          305 t
ORIGIN      
        1 ctgatcgaaa gttgattttc aggaaccgtt ctgacagctt cggctttgaa ctctttagag
       61 taacgttggg tttttctgct cattattagc tccttctgat gccattctat ttcaggaagg
      121 agtgtccgtt aaactcaggc tacctcagtg cactgataat aaaaagacgg agacagatgc
      181 catttacgat aaagttcgga gttcttattt gctttcatgt attctgaaaa aaaacaaaaa
      241 tgttgggctt attcttcatg caccatcatt tgtttctgtg tctgaaaaaa tcgccagaat
      301 tgtgatggca aattactccc gtaactggag taattccgag ttggccagtg cggttttaat
      361 gagtgaatct tctttaaaaa gaagaatgta caaagaggtc ggttctataa gtacatttgt
      421 acataaaata aagcttacag aagctatcag gaaattacga aggacaaata ctcccatttc
      481 tgtcatatca tctgagttag gttacagttc accatcgtat ttttctaaag ttttctttaa
      541 atatttaaag acataccctc aaaacataag gaagaaaaat ggtcggtaat gtcaccaatt
      601 aggaggactt gcagagccgc ttttttacga tactcacccc gtaagtaagc gataattggt
      661 aacttaaacg cccatctgac atagatggac acctgagtat ggaattacag gactggcgaa
      721 aagaacctcg taaaaagtat ttggtgcaaa tcgttggttc ctttgctgaa tttgaacgcg
      781 ccatgcttcg tgaacggaca aagagtggac ttgcagcggc acgccaggac gggttggtgg
      841 atggcgacca aagttaacac cccagcaaca aaaagagatt gtctcgctcg tcacatcggg
      901 gcagaaaaca ggtgctgatg ccgcccgtct gtttcgggtt cacccttcca ccgtggtgcg
      961 tcttctcgcc agacaccgga tgactgagat caaacagaca taatctatgc ggagatttac
     1021 agcagggcat ggttcacaaa tctgacagcg atgaattatc ggcactgcgg gcagaaaatg
     1081 ctcgaatcat caagcccctg ttac
//
LOCUS       DAETQN010000346         1087 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.355, whole genome shotgun sequence.
ACCESSION   DAETQN010000346 DAETQN010000000
VERSION     DAETQN010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.355"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..645)
                     /locus_tag="K0W68_004720"
     CDS             complement(<1..645)
                     /locus_tag="K0W68_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBI3076009.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITD"
     gene            complement(768..>1087)
                     /gene="tabA"
                     /locus_tag="K0W68_004721"
     CDS             complement(768..>1087)
                     /gene="tabA"
                     /locus_tag="K0W68_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBI3076010.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          313 a          247 c          191 g          336 t
ORIGIN      
        1 atccgtaatc gtagaatcaa ctgaatcttt acaactcacc tgcgagttat tcaagtcctg
       61 agaattattt aactcctgcg gaatatttaa tagacgagct atttccttgt tagcttcgcg
      121 ttggtaacat gccagaatct tgttagtaat caggtgtaat gaactctcat cttttctttc
      181 aaaggacttt ccttcataac gattagtcga accatcaggt tgttgaaata taatagccgt
      241 atgtccttga cgagtcccca tagtagtgaa agcggaaagt gtgatttcta tttccacctt
      301 agataaatcc gctcgttccc cttcaggatt attcataaca aacttcatta tctcactgcc
      361 atcattattt ttgatataag atgaggactc aatatgagcc atcatatttc ttcccgcttc
      421 gttggagcgt ttgctaaaat ccacataacc tagagtaaat atttttacca aaaattccaa
      481 aaatgtctta ggatcaagga tatttcttgt gttatcctgc ccctcggcat tacgggcaaa
      541 attattcatt ccaacacgcc aggtatttaa aacttcactt ttttgtttaa aattgtcggc
      601 taatgtaatg tgttgagaat aattcaaatc tgatattttg ctcatgacga actccttgag
      661 ttaataagaa cgtctaagca ttacaaattt tactcacaaa aaaatatcaa aaaacgctct
      721 tttcacaaac tcgccgtaaa cctggagtta tttacggcga aaagtcatca cgccatcagc
      781 atcttcacta ctgctttgcg aacctgggct ggtgcaccca ctgcgcacag cggtttatgc
      841 acttcccccg gataaaacac aacaaaatca ccttcattaa ggataactgt tttctcatca
      901 acgccttccg gcaaaaatgc gatgtcttta tcagctaacc agtcggtatc cggcgtgcct
      961 gcaggttgcg tgctgaaggt catgccttcc tgacctttta acacaatctg aatgtcgaga
     1021 tagcgggcat ggtactccgt acggcgagct tcgtgcggct cggtcatatc ttccgagata
     1081 agataaa
//
LOCUS       DAETQN010000347         1045 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.356, whole genome shotgun sequence.
ACCESSION   DAETQN010000347 DAETQN010000000
VERSION     DAETQN010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.356"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            284..388
                     /locus_tag="K0W68_004722"
                     /pseudo
     CDS             284..388
                     /locus_tag="K0W68_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            728..985
                     /locus_tag="K0W68_004723"
     CDS             728..985
                     /locus_tag="K0W68_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HBI3076011.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          357 a          179 c          198 g          311 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tattgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 ccattagtct gcttcctgtt cttacaataa tcagtattat gtattttatt tatatagcaa
      661 aatatgcttg ttacacctgt cataattagg catggtaaaa ttattgactg aaccctgggg
      721 gaggaagatg cacagtgatt attataatat ggtttttggt gaaaaattgg ccaatatttt
      781 atatgaggct aactctcaat ttttttacga gagaaatgtc atagaagagg cagtcaacgc
      841 tttattctgt gagcgtgaaa taataaacaa caagaacata ataaagaagc tgatgttctt
      901 tttgtcagat gttaatcaca ccaagaaaga tgttgttcag agcgcactta atattatcat
      961 tgacattaca tcaggtgata tttgagattt tacataaaag ataatctctc aaggagtctt
     1021 atctgaaaac gaaatatata gtccc
//
LOCUS       DAETQN010000348         1041 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.357, whole genome shotgun sequence.
ACCESSION   DAETQN010000348 DAETQN010000000
VERSION     DAETQN010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.357"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(88..>1041)
                     /locus_tag="K0W68_004724"
     CDS             complement(88..>1041)
                     /locus_tag="K0W68_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3076012.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDK
                     KTGKGIWKGGDSGINTLMNEGFLTKKDLKDFANNSAGLISKAVNGMVKKGYTDAQIGS
                     RLEDLADDLLLTSKYWQRSAREVQASITQQKAAGQWLTETQ"
BASE COUNT          223 a          254 c          209 g          355 t
ORIGIN      
        1 tgtactccaa tgactagtct aaaaactagt attaagacta tcacttattt aagtgatact
       61 ggttgtctgg agattcaggg ggccagtcta ctgtgtttct gttagccatt gtccggcggc
      121 tttttgctgg gtaatgcttg cctgcacctc tctggctgat ctttgccaat atttagaagt
      181 taagaggagg tcgtccgcta aatcttccag acgactaccg atctgtgcgt cggtatagcc
      241 ctttttcacc attccgttga cagcttttga aattaaacca gcagagttat tggcaaagtc
      301 tttcaaatcc tttttagtta agaatccttc attcattaaa gtgttaatac cagaatcacc
      361 gcccttccat atgcctttcc ctgttttctt atcaactaca tattcagcct cgtcatagct
      421 tttggtagcg cgttccttta catctttacc gatggcatct agtatttttc tgcgtcccgg
      481 atcaccctgg aggctatcga tcccgttgtc atacgcccaa cgctcaagat tattaagtaa
      541 gttgtaatca actccaccaa gccttgcacg ttggattagc tccccttctg tttcagcatt
      601 agcaaagctg ttagccataa attcagaggt tttactaaca gccatcacgc cagcagcagc
      661 cgtagcgcct ccaaataaca gagcgcccat cgtggcggaa ttccctgatc ctcccttaac
      721 gggggcaaat tgagcttttc tgttgccatt aatcttacgt tgttgctgtt gtaggcgttt
      781 catctgacta ttcatacgcg ccaggctgat agccccttgc tcatattgtt ttgctatctc
      841 tctagcctgt gcaatagctt tgtattgttc cgctacagag agacggtgca ttgcactgat
      901 agagcttgat acatcaagga gttttaattc tgctttctct ttacgtgctg ctatttgttg
      961 ctgttgcctt gcctgtgctc ttgctgcggc agcttgtgct ctttgattct gcttttctgt
     1021 tttagctttc tgttgagcaa t
//
LOCUS       DAETQN010000349         1023 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.358, whole genome shotgun sequence.
ACCESSION   DAETQN010000349 DAETQN010000000
VERSION     DAETQN010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.358"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(77..865)
                     /locus_tag="K0W68_004725"
     CDS             complement(77..865)
                     /locus_tag="K0W68_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI3076013.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="K0W68_004726"
     CDS             complement(905..>1023)
                     /locus_tag="K0W68_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBI3076014.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAETQN010000350         1005 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.359, whole genome shotgun sequence.
ACCESSION   DAETQN010000350 DAETQN010000000
VERSION     DAETQN010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.359"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..1005
                     /locus_tag="K0W68_004727"
                     /pseudo
     CDS             1..1005
                     /locus_tag="K0W68_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          248 a          243 c          307 g          207 t
ORIGIN      
        1 cctctgaact tgctgccgcc atgaagcaga ttaaagaact ccagcgcctg ctcggcaaga
       61 aaacgatgga aaatgaactc ctcaaagaag ccgttgaata tggacgggca aaaaagtgga
      121 tagcgcacgc gcccttattg cccggggatg gggagtaagc ttagtcagcc gttgtctccg
      181 ggtgtcgcgt gcgcagttgc acgtcattct cagacgaacc gatgactgga tggatggccg
      241 ccgcagtcgt cacactgatg atacggatgt gcttctccgt atacaccatg ttatcggaga
      301 gctgcccacg tatggttatc gtcgggtatg ggcgctgctt cgcagacagg cagaacttga
      361 tggtatgcct gcgatcaatg ccaaacgtgt ttaccggatc atgcgccaga atgcgctgtt
      421 gcttgagcga aaacctgctg taccgccatc gaaacgggca catacaggca gagtggccgt
      481 gaaagaaagc aatcagcgat ggtgctctga cgggttcgag ttctgctgtg ataacggaga
      541 gagactgcgt gtcacgttcg cgctggactg ctgtgatcgt gaggcactgc actgggcggt
      601 gactaccggc ggcttcaaca gtgaaacagt acaggacgtc atgctgggag cggtggaacg
      661 ccgcttcggc aacgatcttc cgtcgtctcc agtggagtgg ctgacggata atggttcatg
      721 ctaccgggct aatgaaacac gccagttcgc ccggatgttg ggacttgaac cgaagaacac
      781 ggcggtgcgg agtccggaga gtaacggaat agcagagagc ttcgtgaaaa cgataaagcg
      841 tgactacatc agtatcatgc ccaaaccaga cgggttaacg gcagcaaaga accttgcaga
      901 ggcgttcgag cattataacg aatggcatcc gcatagtgcg ctgggttatc gctcgccacg
      961 ggaatatctg cggcagcggg cttgtaatgg gttaagtgat aacag
//
LOCUS       DAETQN010000351         1001 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.360, whole genome shotgun sequence.
ACCESSION   DAETQN010000351 DAETQN010000000
VERSION     DAETQN010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.360"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>1001
                     /locus_tag="K0W68_004728"
     CDS             <1..>1001
                     /locus_tag="K0W68_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HBI3076015.1"
                     /translation="SGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTG
                     SVTVSAVTTAGGMQTVDITLVAGPADASKSVLKNNRSSLKGDFTDSAELHLVLHDISG
                     NPIKVSEGLEFVQSGTNVPYVQVSAIDYSKNFSGEYKATVTGGGEGITTLIPVLNGVH
                     QAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAA
                     DYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSYVYEIRVKSWWVNAGEA
                     FMIYSLAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDMGHYTTEAGFQSNMYW"
BASE COUNT          293 a          221 c          253 g          234 t
ORIGIN      
        1 cagtggtaac ctgatcgaag gtctcactgt gtactttgcc ttaaaaagcg gctctgccac
       61 attaacgtca ttaacagcgg tgaccgatca aaacggaatc gcgacaacaa gcgtgaaagg
      121 agcgatgaca ggtagcgtca cggtaagcgc agtcacgacc gctggtggaa tgcaaacagt
      181 agatataacg ctggtggcag gcccagcaga cgcctcaaag tcagtcctta agaacaatcg
      241 gtcatcattg aaaggagact ttaccgatag tgctgagcta catcttgttc tgcacgatat
      301 atcaggcaat ccgatcaaag tttctgaagg gctggaattt gtacagtcag gtaccaacgt
      361 gccctatgtg caagttagtg caattgacta cagtaaaaat ttctcaggcg agtacaaagc
      421 cactgttaca ggaggcggag agggtatcac aacgctgatc cctgtattga atggtgttca
      481 tcaagctggt ctgagtacca caatacaatt cactcgcgca gaagacaaaa taatgagcgg
      541 tacagtatca gtcaatggta ctgacctacc gacaactaca ttcccttcgc aggggttcac
      601 cggggcgtat tatcagttga ataatgacaa ctttgcccca ggaaaaacgg cggctgatta
      661 tgagttttca agctctgcct cctgggtcga tgttgatgct accggtaaag tgacatttaa
      721 aaatgtcggc agcaattcgg aaaggattac ggcgacacca aaatcaggag gccctagcta
      781 tgtatacgaa atccgtgtga agagttggtg ggtgaacgcc ggcgaggctt tcatgatata
      841 cagccttgct gaaaattttt gcagcagcaa tggctacacg ctccccagag caaactattt
      901 aaaccacagt agttcccgag gcatcgggtc actgtacagt gaatggggag atatggggca
      961 ttacacgact gaagctggtt ttcaatcaaa tatgtattgg t
//
LOCUS       DAETQN010000352          987 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.361, whole genome shotgun sequence.
ACCESSION   DAETQN010000352 DAETQN010000000
VERSION     DAETQN010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.361"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            211..>987
                     /locus_tag="K0W68_004729"
     CDS             211..>987
                     /locus_tag="K0W68_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HBI3076016.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRD"
BASE COUNT          220 a          300 c          258 g          209 t
ORIGIN      
        1 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
       61 atccgggggg tgatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
      121 tcaggcactt ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta
      181 tctggttaac ccatatacaa gggagacaga atgaccgaat ccagcgatta cgaatccgtc
      241 caggtcttta tcggcgttga tgtcggtaaa gatacgcatc acgctgttgc cattaatcgt
      301 tcaggtaaac gcctgttcga taaagcatta cccaacgacg aaaacaaact caggtcgcta
      361 atatctgacc tgaaacaaca tggtcagata ctgctggttg ttgatcagcc agctaccatc
      421 ggtgcgttac ctgtcgccgt tgcccgctca gaaggagtcc ttgtcggata cctccctgga
      481 ctggccatgc gccgcatagc cgacttacac gccggtgaag ctaaaactga tgctcgtgac
      541 gctgccatca ttgccgaagc tgcccgtacc ctgcctcacg cgctacgcac gctgaaactg
      601 gctgacgagc aaatcgccga actctccatg ctctgcggct tcgatgatga tcttgccgca
      661 cagacaacgc aggccagcaa ccgtatccgc ggccttctga cccagataca tccggcactg
      721 gagcgcgttc tcggtccgag acttgagcac ccggcggtac tcgatcttct ccagcgatat
      781 ccctcaccag aaaaactcgc ttcgctgggt gagaagaagc tggcagccca gctctgcaaa
      841 cttgcgcctc gtctgggtaa acgccttgca gcagacatag ctcaggcact ggccgaacaa
      901 accgtcgtcg ttcccggcac gaatgccgct gccgtagtac tgccacgtct ggcactccag
      961 ctcatcacgc tgcgtaagca aagagac
//
LOCUS       DAETQN010000353          977 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.362, whole genome shotgun sequence.
ACCESSION   DAETQN010000353 DAETQN010000000
VERSION     DAETQN010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.362"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            15..>977
                     /gene="istA"
                     /locus_tag="K0W68_004730"
     CDS             15..>977
                     /gene="istA"
                     /locus_tag="K0W68_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBI3076017.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVSFD"
BASE COUNT          233 a          258 c          260 g          226 t
ORIGIN      
        1 attcaggaga gcttatggtc acttttgaga cagttatgga aattaaaatc ctgcacaagc
       61 agggaatgag tagccgggcg attgccagag aactggggct ctcccgcaat accgttaaac
      121 gttatttgca ggcaaaatct gagccgccaa aatatacgcc gcgacctgct gttgcttcac
      181 tcctggatga ataccgggat tatattcgtc aacgcatcgc cgatgctcat ccttacaaaa
      241 tcccggcaac ggtaatcgct cgcgagatca gagaccaggg atatcgtggc ggaatgacca
      301 ttctcagggc attcattcgt tctctctcgg ttcctcagga gcaggagcct gccgttcggt
      361 tcgaaactga acccggacga cagatgcagg ttgactgggg cactatgcgt aatggtcgct
      421 caccgcttca cgtgttcgtt gctgttcccg gatacagccg aatgttgtac atcgaattca
      481 ctgacaatat gcgttatgac acgctggaga cctgccatcg taatgcgttc cgcttctttg
      541 gtggtgtgcc gcgcgaagtg ttgtatgaca atatgaaaac tgtggttctg caacgtgacg
      601 catatcagac cggtcagcac cggttccatc cttcgctgtg gcagttcggc aaggagatgg
      661 gcttctctcc ccgactgtgt cgccccttca gggcacagac taaaggtaag gtggaacgga
      721 tggtgcagta cacccgtaac agtttttaca tcccactaat gactcgcctg cgcccgatgg
      781 ggagcactgt cgatgttgaa acagccaacc gccacggtct gcgctggctg cacgatgtcg
      841 ctaaccaacg aaagcatgaa acaatccagg cccgtccctg tgatcgctgg ctcgaagagc
      901 agcagtccat gctggcactg cctccggaga aaaaagagta tgacgtgcat cctggtgaaa
      961 atctggtgag cttcgac
//
LOCUS       DAETQN010000354          971 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.363, whole genome shotgun sequence.
ACCESSION   DAETQN010000354 DAETQN010000000
VERSION     DAETQN010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.363"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..133)
                     /locus_tag="K0W68_004731"
     CDS             complement(<1..133)
                     /locus_tag="K0W68_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3076018.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     "
     gene            141..572
                     /gene="tnpA"
                     /locus_tag="K0W68_004732"
                     /pseudo
     CDS             141..572
                     /gene="tnpA"
                     /locus_tag="K0W68_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          297 a          221 c          184 g          269 t
ORIGIN      
        1 cctcatgatt ctcattctga cgtgccagag cacgattgaa aacgaaacga catgcgcctg
       61 cgaagcgcct catttcacgc tcctgttgac caccgggtct taactggaat ttaaatgctt
      121 gtagtcgttt cacaggccaa atgatacttt ggtctgtgaa aaaagaaacc gatatccggc
      181 gtggaaggca ttgtgttttc ctgatgcatg ttcacctggt ctttgtcacc aaataccgac
      241 gtcagatttt tgaccatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg
      301 ctgattttga agctgaactg gttgaaatgg atggcgaacc agatcacgtc catttgttaa
      361 ttaactatcc tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa ggcgtatcgg
      421 gtaggtgact gcgacgagat cgaccagata ttgcagtcag gtattactac aaaggcgttt
      481 tgtggagtgt tggctatttt gccagtagct gcggaggtgc gccaatatcc gtcatccgcc
      541 aatacattga acaacagcaa acacctggtt aggtggaaaa ccacgcctta tatccctgcc
      601 ctgaaggacg gggttttacg gcgcaccgga taagcctgga gattatctac accagcatgg
      661 acatcattgt actatgcccc gtacatgccc catcacatcc tcaacatcaa agtattagag
      721 ccttgcaaac cattatgcgt ctcagtttgt ctcatctatc ttacgcaaaa aaaaatagaa
      781 ctaatatcaa gcggcgctat attgcgtatt aaataaaacc acctcaatta cattaataaa
      841 tctaaacaaa catcatgcaa ataatttcca tggttatttc taggctattt aaattaaata
      901 aataaattat gagtcaatca taaataacaa cctttaatat aatttaacta ccaaatttat
      961 attcgttcac a
//
LOCUS       DAETQN010000355          962 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.364, whole genome shotgun sequence.
ACCESSION   DAETQN010000355 DAETQN010000000
VERSION     DAETQN010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.364"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..400
                     /locus_tag="K0W68_004733"
     CDS             <1..400
                     /locus_tag="K0W68_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HBI3076019.1"
                     /translation="DVINAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSL
                     AAVLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTV
                     LDGKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            574..>962
                     /locus_tag="K0W68_004734"
     CDS             574..>962
                     /locus_tag="K0W68_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3076020.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHT"
BASE COUNT          328 a          177 c          190 g          267 t
ORIGIN      
        1 tgatgtgatt aatgccattg tcaaaccgtt ctatgaacgt cagatgagta agtcagataa
       61 tccaattgat aaagttgtaa ggtcaaaggt ggaacccaca gaccaagcat tgactggtga
      121 catgaactct ttagccgctg ttttatgggc tatggttcag ggcaaatcaa aagatgttcc
      181 acagttttca cctgaagcat taaataatct gaaaggtcga gtggaaacat tcacacaaga
      241 tttaaaggtt atcaaaccgt cagtaaatat tcagcctcag acaatcaata tcacaactca
      301 gacggtactt gatggaaagg ttattgatga aagaaccaca tcgcatatta atcgtatgca
      361 ggaagatacc ttgattagct cagcttatcc agaggaataa acaatgttag agctactaat
      421 atttattgcc acacatggca atctattaat ctttatctag tatgacgatt caactatgaa
      481 tagtcgaatg ctacgtgaaa tcttctgaac agataccaac atcaaaaatt agatttcacg
      541 tagctcacgc ttaacagaat aggaataaca acaatgtttg acacaaagca aattagatac
      601 tcaggtaaag atggtatcta ttttcacctt agagataatg tggaatcttt tcttgcctta
      661 acagctactg aacagatgga ctatgactca ccgatgatgg tgacaactca ggctatgcaa
      721 tcggggcaaa ctatcactga taatgttcag agagcaccta agaccatctc tattagcggt
      781 gttgtagtgg ttgattcgac gggttcagtt ctattaacca gacaggggca acttgtagaa
      841 acctttattt ctacggttga acaatggcga gaccagaagc aaatcatcac tattgtttgt
      901 agagatggaa tagctatcac tgatagtatc attactcagt ttaaagcgag taaagagcat
      961 ac
//
LOCUS       DAETQN010000356          958 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.365, whole genome shotgun sequence.
ACCESSION   DAETQN010000356 DAETQN010000000
VERSION     DAETQN010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.365"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>958
                     /locus_tag="K0W68_004735"
     CDS             <1..>958
                     /locus_tag="K0W68_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HBI3076021.1"
                     /translation="ILGYQANSNGKVIVEKGGEWLIKNNDSSIEFQIGNQGAGEATIR
                     EGGLITAENTIIGGNATGIGTLNVQDQDSVITVRRLYNGYFGNGTVNISNNGLINNKE
                     YSLVGVQDGSHGVVNVTDKGHWSFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIIT
                     AGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGENGV
                     GNVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLM
                     DATSSFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIGGNASG"
BASE COUNT          272 a          172 c          266 g          248 t
ORIGIN      
        1 attttaggat atcaagcgaa cagtaatggt aaggttatcg ttgaaaaggg tggcgagtgg
       61 ctaataaaaa ataatgattc ctcaattgaa tttcaaattg gtaatcaagg agctggggag
      121 gcgactattc gcgagggtgg gctgattacg gctgaaaata cgattatcgg tggcaatgcc
      181 accggtatcg gaaccctgaa tgtgcaggat caagactctg tcatcacggt acgcagactc
      241 tataatgggt atttcggtaa tggcacagtc aatatttcca ataatggtct gattaataac
      301 aaagaatatt cattggtggg cgttcaggac ggttcccacg gtgtcgtcaa cgtgaccgat
      361 aaagggcatt ggagtttcct cggaacaggc gaagctttcc gctatatcta tatcggtgat
      421 gctggtgacg gtgaacttaa tgtctcgcgt gaaggtaaag tagattcggg aattatcact
      481 gcggggatga aagaaacagg cacaggcaac attactgtta aggataagaa ctccgttatc
      541 actaatctcg gaactaatct tggttatgac ggccacggcg aaatgaatat cagtaatgag
      601 gggcttgttg tcagcaacgg aggaagctca ctcggttatg gagaaaacgg cgtcgggaat
      661 gtcagcatca ccacgggggg gatgtgggag gtcaataaga atgtctatac caccattggt
      721 gttgcgggcg tcggaaacct caatattagc gatggcggta agtttgtatc gcaaaatatt
      781 acttttttgg gcgataaagc aagcggtatc ggcacactga acctgatgga tgcgacatca
      841 tcgttcgata ctgtgggtat caatgtcggt aattttggta gcggtatcgt aaatgtcagt
      901 aatggtgcca cccttaattc aacgggctat ggatttatcg gaggaaatgc ctccggta
//
LOCUS       DAETQN010000357          958 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.366, whole genome shotgun sequence.
ACCESSION   DAETQN010000357 DAETQN010000000
VERSION     DAETQN010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.366"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            6..958
                     /locus_tag="K0W68_004736"
                     /pseudo
     CDS             6..958
                     /locus_tag="K0W68_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          236 a          279 c          293 g          150 t
ORIGIN      
        1 cgtagggtga agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca
       61 cgcagagtca gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga
      121 caacagaata cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg
      181 gcagggcatc ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg
      241 acgggctgga aatgcgccgc aaatatgatg aatccggccg tctgattcag gaaaccgccc
      301 ctgacggcga tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa
      361 cggaagatgc caccggcagc cggaaaacca tgacatggag ccgttacggt cagttgctga
      421 ccgtcactga ctgttccggt tatgtaaccc gttatgacca tgaccgtttt ggtcaggtga
      481 cggcggtgca ccgcgaggaa gggctgagcc agtaccgcgc atacgacagc cgtggacagt
      541 taattgcggt gaaagacacg cagggccatg aaatgcggta tgaatacaac gccgccggtg
      601 acctgaccgc cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg
      661 gaaaagccat ctgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg
      721 gacgggtcat ccgtctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac
      781 tcgaccggct gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc
      841 tgaccggcaa acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg
      901 aagcagaccg cctcacgcac cgcaccgtga agggtgaaac cgcagagcgg tggcggta
//
LOCUS       DAETQN010000358          958 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.367, whole genome shotgun sequence.
ACCESSION   DAETQN010000358 DAETQN010000000
VERSION     DAETQN010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.367"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>958
                     /locus_tag="K0W68_004737"
     CDS             <1..>958
                     /locus_tag="K0W68_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBI3076022.1"
                     /translation="FLDAGGLRDIEVEAVTKMLACGTRILGVKEYNCDKPECPHVRYV
                     TNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTLWPVFESNRWLLNDV
                     CRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGCLNKHGQWKKLSF
                     LKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQWRSLVLKAGGKYWHVYMSK
                     KTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKTGETATETLTQRELV
                     ARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKPE"
BASE COUNT          221 a          240 c          310 g          187 t
ORIGIN      
        1 ttcctggatg cgggcggtct gcgcgatatc gaggttgagg ctgtcacaaa aatgctggcc
       61 tgcggcacgc ggatactggg agtgaaggaa tacaactgcg ataaacctga gtgcccccac
      121 gtcagatacg tcactaactc atgcggcagc cgtgcctgcc cgtcctgcgg aaaaaaggcc
      181 acagacctgt ggatagcgac acagctgaat cgtcttcctg actgcgactg ggtacatctg
      241 gtcttcaccc tgccggacac gctgtggccg gtgttcgaaa gcaaccggtg gctgctgaat
      301 gacgtgtgcc gtctggcggt ggagaatctg ctgtatgccg cccggaaacg ggggcaggaa
      361 cccggtatct tctgcgccat ccacacgtat ggccgtcgtc tcaactggca tccgcatgta
      421 catgtgtctg taacctgtgg atgtctgaat aagcatggtc agtggaaaaa gctgagcttc
      481 ctgaaagacg cgatgcgttc gcggtggatg tggaatatgc ggcaacggct tctgaaagcg
      541 tggtcagagg ggctggcgat gccggagtcg ttgtcacata tcacgacgga atcacagtgg
      601 agaagcctgg tgctgaaagc cggaggaaaa tactggcatg tgtacatgtc gaaaaaaacg
      661 gccggcggga ggaatacggc gcgctacctg ggtcgttatc tgaagaagcc gccgatagcg
      721 gcctcccggc tggcacatta caacggaggg gcgagcctga gcttccgtta cctggaccac
      781 aaaacgggag aaacggcgac ggaaacgctg acacagcgtg agctggtcgc gaggctgaaa
      841 cagcacatcc cggagaagtt ttttaagatg gtgaggtact tcgggttcct tgccaaccgg
      901 gtgtgtggag agaagctgcc gcaggtgtac cgtgctctgg ggatggataa accggaac
//
LOCUS       DAETQN010000359          938 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.368, whole genome shotgun sequence.
ACCESSION   DAETQN010000359 DAETQN010000000
VERSION     DAETQN010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.368"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            84..>938
                     /locus_tag="K0W68_004738"
     CDS             84..>938
                     /locus_tag="K0W68_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HBI3076023.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIY"
BASE COUNT          253 a          241 c          255 g          189 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttat
//
LOCUS       DAETQN010000360          934 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.369, whole genome shotgun sequence.
ACCESSION   DAETQN010000360 DAETQN010000000
VERSION     DAETQN010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.369"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(196..>934)
                     /locus_tag="K0W68_004739"
     CDS             complement(196..>934)
                     /locus_tag="K0W68_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBI3076024.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          258 t
ORIGIN      
        1 tgcttctccg ataaaatgtc agagaagcat gattacgtgg ttattgtctg ttgatgactt
       61 gttgaccgag ccgtcatcat tctcaatgca ttagttaaat catttgtgat ttttaaggat
      121 tatcctgtct atgattttct acttagaagg atatggcact aactatgcat cttatagttt
      181 actgctggca tctatttaaa atagcaattg agtcaaatag cccatcaaga tagccatacc
      241 taatataacc gtcaggaatg cagctatcat cggcattctg aacattgatt taagtaatat
      301 cacttccgtc aggcttgcgc cggcactgcc gatgattaat gccattaatg ctcccagacc
      361 cattcctttt gtcatcagaa cagatgccag agggataacc gcctcagccc ggatatacag
      421 cggaataccg acaacggcgc ttaatgggat ggcgaaggga ttatctgctc ctgcatgagc
      481 ggcaatccac gctgagggaa taaagccata aataaaagat cctattaata ctcctaacaa
      541 aagatatggc agtacatctt taaactgttg caacgcatct ttggtggcta gttttatggg
      601 attaatactt actacagctt tggtactgca acaggtaact acggggttct caatggcttt
      661 agccgctgga ctacaacaac tcacttttat gggcgtatat gtcgttcccg gcgaagtttt
      721 tgctggagca caacaatttg ctgatgagct tttactggca atgatatgac gttcaaaccc
      781 cagagaatcc aggataatac tggcaagaac ggagacgccg gctgcgataa tcgcgtacaa
      841 caaggtgact ttccaaccaa aggtcatcca cattagtccg acgataatgg gattaagtaa
      901 tggtgaaaca aataaaaaag tgagagtcgg acca
//
LOCUS       DAETQN010000361          912 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.370, whole genome shotgun sequence.
ACCESSION   DAETQN010000361 DAETQN010000000
VERSION     DAETQN010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.370"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..757)
                     /gene="ipaH3"
                     /locus_tag="K0W68_004740"
     CDS             complement(<1..757)
                     /gene="ipaH3"
                     /locus_tag="K0W68_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HBI3076025.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLSQ"
BASE COUNT          268 a          145 c          224 g          275 t
ORIGIN      
        1 gttgcgacag agactccctg atccgtgagg acagaggatt gtcttcgagg atgatagtgc
       61 aggtcggatc aaggctaagt atattttccg gaatgtatgt gatgcgattc tcgcggcacc
      121 ggaaaaatat ctcggtttcc tctgagtgat gatttcttac aggtacggct ggtaggcttt
      181 ccagaagatt agtacttaca tcgagcgctt ccagtgattc aggtaactca gggagaaatg
      241 tcagctggtt atttcttact gagagcactt ccagcgatgt aggtaattca ggaagcatgg
      301 ttagctgatt gttatctgca ttaatatatt ccagcaatgc aggcaattca ggaagcatgg
      361 ttagttggtt gttatctaca tcaagatgtt tcagagatgc gggtaattca ggaagtgttg
      421 acaggtgatt gtcacaggcg tcaaggtatt ccagcgatgc tggcaattct ggtaatgata
      481 ttagggcatt ctgagtaatt tccagaacag tgatttgagg tggtaagttg tcaggtagcg
      541 aggacagatt taaacgattc agttgaagct cactgaactg attgatgaga cattctttaa
      601 gtagggagac cgcttcattc cgattttcac cggggagcgc ttgtttttcc catttatccc
      661 atgctgaaaa gtaatcagca tatgtaccgg aaatagtgtt ataaaaagaa ttttgggaca
      721 atgaaaagtt attatttatc ggtaacatga tggacattca gttaaagccg atggacaaat
      781 ggctttgtta aaagaaataa gtaggagttt ttatgaagtt cattccatga aatgaacttc
      841 agcacttttt tattataaaa ggacaaacaa aattggctgt aaaaaaacga tactgtttca
      901 gttaactgtg aa
//
LOCUS       DAETQN010000362          901 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.371, whole genome shotgun sequence.
ACCESSION   DAETQN010000362 DAETQN010000000
VERSION     DAETQN010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.371"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..372
                     /locus_tag="K0W68_004741"
                     /pseudo
     CDS             1..372
                     /locus_tag="K0W68_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            553..702
                     /locus_tag="K0W68_004742"
                     /pseudo
     CDS             553..702
                     /locus_tag="K0W68_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(729..>901)
                     /locus_tag="K0W68_004743"
     CDS             complement(729..>901)
                     /locus_tag="K0W68_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3076026.1"
                     /translation="VIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKA
                     YYASIGNDDLAA"
BASE COUNT          230 a          184 c          266 g          221 t
ORIGIN      
        1 atgaaattgg cctgcgttac aacagccacc ggacaaaacg agaagaaaac cttgtgatga
       61 gtggcgacga gtttatggaa aggttctcct ggcatgtggc ggataagggg ttccggatgg
      121 tgatacgggg tcctgagtcc ggtgaagcgg cgattactgg aagatgtggt gtacgccata
      181 acggagacag tgagaaaaac ggcgaggcaa atcacaagga gagggatgta tcagcagtga
      241 ctgaaggttg acccgctgaa gtgcgagctg tgcggaggtc agatgcggtt tacggggctg
      301 aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc tcgaatacgg
      361 tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa cggagcaaaa
      421 aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct ataccgatta
      481 cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt aatcttttgg
      541 ggatttaaat tcctatcgat ggacatgaat gcaggctata taagagcagc gcgcatccac
      601 ttacccaatg cggttgagaa aatagccttc gatcgctttc atgtggcgaa acaaccgggt
      661 gaggtggtgg ataaaacccg ccagaatgaa ccgccccggg tttcctggag agtgttttat
      721 ctgtgaactc aggctgccag atcatcgttt ccgatggaag cataataagc tttttctgct
      781 tctgccggag gagtatggcc cagcctttcc agcaatcgtc gattgttata ccagtccacc
      841 cacgtgagtg tggccagttc cacttctgca cggtttttcc agctcttacg gtgtattacc
      901 t
//
LOCUS       DAETQN010000363          876 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.372, whole genome shotgun sequence.
ACCESSION   DAETQN010000363 DAETQN010000000
VERSION     DAETQN010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.372"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>876
                     /gene="cadA"
                     /locus_tag="K0W68_004744"
     CDS             <1..>876
                     /gene="cadA"
                     /locus_tag="K0W68_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HBI3076027.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKELH"
BASE COUNT          238 a          220 c          212 g          206 t
ORIGIN      
        1 aacttctcac cgatttacga aggtaaatgc ggtatgagcg gtggccgtgt agaagggaaa
       61 gtgatttacg aaacccagtc cactcacaaa ctgctggcgg cgttctctca ggcttccatg
      121 atccacgtta aaggtgacgt aaacgaagaa acctttaacg aagcctacat gatgcacacc
      181 accacttctc ctcactacgg tatcgtggcg tccactgaaa ccgctgcggc gatgatgaaa
      241 ggcaatgcag gtaaacgttt gatcaacggt tccattgaac gtgcgatcaa attccgtaaa
      301 gagatcaaac gtctgagaac ggaatctgat ggctggttct ttgatgtatg gcagccggat
      361 catatcgata cgactgaatg ctggccgctg cgttctgaca gcacctggca cggcttcaaa
      421 aacatcgata acgagcacat gtatcttgac ccgatcaaag tcaccctgct gactccgggg
      481 atggaaaaag acggcaccat gagcaacttt ggtattccgg ccagcatcgt ggcgaaatac
      541 ctcgacgaac atggcatcgt tgttgagaaa accggtccgt ataacctgct gttcctgttc
      601 agcatcggta tcgataagac caaagcactg agcctgctgc gtgctctgac tgacttcaaa
      661 cgtgcgttcg acctgaacct gcgtgtgaaa aacatgctgc cgtctctgta tcgtgaagat
      721 cctgaattct atgaaaacat gcgtattcag gaactggctc agaatatcca caaactgatt
      781 gttcaccaca atctgccgga tctgatgtat cgcgcatttg aagtgctgcc gactatggta
      841 atgactccgt atgctgcgtt ccagaaagag ctgcac
//
LOCUS       DAETQN010000364          866 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.373, whole genome shotgun sequence.
ACCESSION   DAETQN010000364 DAETQN010000000
VERSION     DAETQN010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.373"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..228)
                     /locus_tag="K0W68_004745"
     CDS             complement(<1..228)
                     /locus_tag="K0W68_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBI3076028.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAP"
     gene            complement(225..332)
                     /locus_tag="K0W68_004746"
                     /pseudo
     CDS             complement(225..332)
                     /locus_tag="K0W68_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(344..>866)
                     /locus_tag="K0W68_004747"
     CDS             complement(344..>866)
                     /locus_tag="K0W68_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBI3076029.1"
                     /translation="WCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFNSETV
                     QDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRSPESN
                     GIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQQ
                     ASNGLSDNRCLEI"
BASE COUNT          187 a          267 c          207 g          205 t
ORIGIN      
        1 cggggcaagt tccggcatct cattacacaa cagcacgggg tcaatcactc ctgaaacacg
       61 ggcctgtctg cagatgcctg tgtagatatc ccgatacgct cgtacagaaa aaatgtccgc
      121 cggtagtgtg atcagaatat ccatcacttc atgatctgcc ccacgcagaa agaatgcgcc
      181 aatgacagcg ccttccaggt catcattacg ccagactggt gttgtcatgc agccacacct
      241 ctgatacaag aacggtagct gggccagttg aacgacaacc agttgcgccc cccgtctgtg
      301 atcctgtcgg caatgcgggg gctgatgaac gctggatttg cccctatatt tccagacacc
      361 tgttatcact taacccatta ctggcttgct gccgtagata ttcccgtggc gagcgataac
      421 ccagtgcact atgcggatgc cattcgttat aatgctcgaa cgcctctgca aggttctttg
      481 ctgccgttaa cccgtctggt ttgggcatga cactgatgta gtcacgcttt atcgttttca
      541 cgaagctctc tgctatgccg ttactctccg gactccgcac cgccgtgctc ttcggttcaa
      601 gccccaacat ccgggcaaac tgccgtgttt cattagcccg gtagcatgaa ccattatccg
      661 tcagccactc tactggagac gccggaagct cgttgccgaa gcggcgttcc accgctccca
      721 gcatgacgtc ctgtactgtt tcactgttga agccgcccgt agtgaccgcc cagtgcagtg
      781 cctcacggtc acagcagtcc agcgcgaacg tgactcgcag tttttctccg ttatcacagc
      841 ggaactcgaa cccgtcagag caccat
//
LOCUS       DAETQN010000365          854 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.374, whole genome shotgun sequence.
ACCESSION   DAETQN010000365 DAETQN010000000
VERSION     DAETQN010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.374"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..854
                     /locus_tag="K0W68_004748"
                     /pseudo
     CDS             1..854
                     /locus_tag="K0W68_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          237 a          209 c          228 g          180 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      601 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      661 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      721 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      781 gatcgaggtt cacagtactg cgcatacgat taccgggtca tacaggagca gtctggtctg
      841 aaaacatcaa tgtc
//
LOCUS       DAETQN010000366          796 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.375, whole genome shotgun sequence.
ACCESSION   DAETQN010000366 DAETQN010000000
VERSION     DAETQN010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.375"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..742)
                     /locus_tag="K0W68_004749"
     CDS             complement(<1..742)
                     /locus_tag="K0W68_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBI3076030.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQLAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          266 a          138 c          161 g          231 t
ORIGIN      
        1 cacataaaca ggtaaagacc ataaatatgg tgaaagtaaa aaagaaggaa aatacagcaa
       61 tggaaaaaat tttatcggag aacaggaata aatctaaaag atagattgct gaaatacaga
      121 ctccactttt acatgcgcga caaaaattac tgccttgatg aaaaaaccag aaagggaaga
      181 acatcactgc aaatacccaa aaaatagcaa agtaataatg atcactggaa gacgctgatt
      241 cactttctga aggattagtc ggatattggt gttgtgaagt gagttggtta ttcaaagcgt
      301 ttatagctag ctctaatccc tgagctaatt tttgttgttt aaatgcgggt atcatgttgc
      361 tacggatgat atctccagcc agagcatcgg ttaccttttc ttccagccca taacctacct
      421 ggatgcggac agtacgatct gaccaggcaa caatgatcag tatcccatca ttacgtttgg
      481 catctcctaa acgccaattg tcaaaaactc ttgtagcata ttgttcaatt gtctcgtctt
      541 tggttgtggg aacaattaat acagcaagtt gcacttgggt attattgaaa atagattcta
      601 acttgtgcgt cagttctttc ttttcttgtg agtttaagct actcgtaaga tccgtaactt
      661 gttgctgtaa ttgtgggaca atagttggtt gggctgcaac aaaggaacac acgaaagtga
      721 acattagtat gaaatagcgc attataaatt tctcattaaa tctggataaa taaattgacg
      781 gcggcatcgg taattt
//
LOCUS       DAETQN010000367          793 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.376, whole genome shotgun sequence.
ACCESSION   DAETQN010000367 DAETQN010000000
VERSION     DAETQN010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.376"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>793
                     /locus_tag="K0W68_004750"
     CDS             <1..>793
                     /locus_tag="K0W68_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HBI3076031.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAV
                     KLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGEDDKFDNFMRQHRFSK
                     VNFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRS
                     DDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSSQ"
BASE COUNT          282 a          116 c          135 g          260 t
ORIGIN      
        1 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
       61 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      121 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      181 ggaattaact attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa
      241 taacattata aaaatgattg atacattgcc agataatgca atgatattga aatccgttct
      301 ggcagtaaaa ctggtgatgc aattaaaaat tctcaatatt gttaataaaa actttattga
      361 gaatatgaaa aaaacattta gccattgtcc ttatataaaa gatccaatta tacgtagtta
      421 tatacattct ggggaagatg acaagttcga taattttatg cgtcaacatc gattcagtaa
      481 ggtgaatttc gatacccaac agatgatcca ttttattaac agatttaata tgaataaagg
      541 gctgattgat aaaaataaca atttttttat ccaacttatc gatcaggcct tacgatcaac
      601 ggatgatatg atcaaagcaa atgcctggta tctttataaa gagtggattc gtagtgatga
      661 tgtttcacct atatttatag aaactgaaga aaagttaaga acctttaaca ctaataaatt
      721 aacacgaaac gataatatat ttatcctgtt ctcctctgtc gatgatgggc ctgttatggt
      781 ggtaagctcc cag
//
LOCUS       DAETQN010000368          777 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.377, whole genome shotgun sequence.
ACCESSION   DAETQN010000368 DAETQN010000000
VERSION     DAETQN010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.377"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..345
                     /locus_tag="K0W68_004751"
     CDS             <1..345
                     /locus_tag="K0W68_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBI3076032.1"
                     /translation="GADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSL
                     VPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVI
                     ADKAAAAELALG"
     gene            complement(407..>777)
                     /locus_tag="K0W68_004752"
     CDS             complement(407..>777)
                     /locus_tag="K0W68_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBI3076033.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          210 a          190 c          177 g          200 t
ORIGIN      
        1 ggtgcagatg gtcatttttg tggcaatttg ccgaatacga cccatttcca tgagcaaacc
       61 gtggagttcc cgatccaggg agagatggtt gatattgtgg cgcatggtga gttgggcggc
      121 gacttttcgt tagtgcctga cagctacgtg acgatggggc cgaaaagcat catggcggcg
      181 aaaaacttgc tgataattgt cagtggcgca ggtaaagcgc aggcactgaa aaatgtgctg
      241 caaggtccgg ttaccgaaga tgttccagct tccgtgttgc agctgcatcc ttcgctgata
      301 gtaatcgctg ataaagccgc tgccgcagaa ttagcgctcg gttaagctga agaaataagg
      361 ccggatgtat gaattacgct ccggcctgtt ctcattattt aaataactac gaaaaatcac
      421 cccacagtct cagcttgccg cagtggagac agcggaagag ataaccctga caatcaccac
      481 ccttttgcag gcatttagct agatcagaat ttctaatacc gaaattctca caatcttctt
      541 caaggttggc aaattcatcg aggcgatctt ttatatcatt ccagcccaca tagccgataa
      601 aagcacagaa atcgccacaa tgcgcaagcc agaattcctg ttgccatcca tgataacctg
      661 gcgtgcgttc aaccaactct ttcagcattt catcaggata tgtgttctta ataccggcaa
      721 attcgtcctc ttcatcatac tcaaattcaa caccttctat gctggcatca tcctgaa
//
LOCUS       DAETQN010000369          749 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.378, whole genome shotgun sequence.
ACCESSION   DAETQN010000369 DAETQN010000000
VERSION     DAETQN010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.378"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            584..>749
                     /locus_tag="K0W68_004753"
     CDS             584..>749
                     /locus_tag="K0W68_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBI3076034.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVI"
BASE COUNT          212 a          135 c          164 g          238 t
ORIGIN      
        1 cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga
       61 acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa
      121 accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg
      181 agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac
      241 aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa
      301 ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg tcagggatta
      361 aaagatgaac gtaaatttat ttaacgcatt aattttaaag ggttttattg tttgttgacg
      421 aaaacaggaa tcgtgttcgg tctcttttta tctgttaaaa gccagaagca tttccttcgc
      481 tgactttata gtcaaccata acacacactc tactgtctga gtccaacgtt ttttaacatt
      541 cttgttaaaa ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc
      601 cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg gcgatataca
      661 cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc cgtggcgaga
      721 tttacttatc tcagtgggag acgttatcg
//
LOCUS       DAETQN010000370          747 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.379, whole genome shotgun sequence.
ACCESSION   DAETQN010000370 DAETQN010000000
VERSION     DAETQN010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.379"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(28..>747)
                     /locus_tag="K0W68_004754"
     rRNA            complement(28..>747)
                     /locus_tag="K0W68_004754"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          166 a          247 c          164 g          170 t
ORIGIN      
        1 catccgccaa aacatcttcg gcgttgtaag gttaagcctc acggttcatt agtaccggtt
       61 agctcaacgc atcgctgcgc ttacacaccc ggcctatcaa cgtcgtcgtc ttcaacgttc
      121 cttcaggacc cttaaagggt cagggagaac tcatctcggg gcaagtttcg tgcttagatg
      181 ctttcagcac ttatctcttc cgcatttagc taccgggcag tgccattggc atgacaaccc
      241 gaacaccagt gatgcgtcca ctccggtcct ctcgtactag gagcagcccc cctcagttct
      301 ccagcgccca cggcagatag ggaccgaact gtctcacgac gttctaaacc cagctcgcgt
      361 accactttaa atggcgaaca gccataccct tgggacctac ttcagcccca ggatgtgatg
      421 agccgacatc gaggtgccaa acaccgccgt cgatatgaac tcttgggcgg tatcagcctg
      481 ttatccccgg agtacctttt atccgttgag cgatggccct tccattcaga accaccggat
      541 cactatgacc tgctttcgca cctgctcgcg ccgtcacgct cgcagtcaag ctggcttatg
      601 ccattgcact aacctcctga tgtccgacca ggattagcca accttcgtgc tcctccgtta
      661 ctctttagga ggagaccgcc ccagtcaaac tacccaccag acactgtccg caacccggat
      721 cacgggtcca cgttagaaca tcaaaca
//
LOCUS       DAETQN010000371          745 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.380, whole genome shotgun sequence.
ACCESSION   DAETQN010000371 DAETQN010000000
VERSION     DAETQN010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.380"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..723
                     /locus_tag="K0W68_004755"
     CDS             <1..723
                     /locus_tag="K0W68_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HBI3076035.1"
                     /translation="SNNLSWFIYKYDELAELAAVIDRLYEFHQLTEQRPTNKPKNCQH
                     AVQVANASIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTLLKTLSHCWPWFKGD
                     ISSPADSWYVSQTPLIKSGLLKEIICKALPLPVDDKSLSEVLHQVGLGKLAARIHDHD
                     RWGDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLRLVREKLPTSGVI
                     MVTHQPGVWNLADDICDISTVL"
BASE COUNT          214 a          163 c          164 g          204 t
ORIGIN      
        1 tcgaacaatt taagctggtt tatttataaa tatgacgaac ttgctgaact ggctgcggtt
       61 atcgatcgct tgtatgagtt ccatcaactc actgaacagc gccctacgaa taagcctaaa
      121 aattgccaac atgcggtaca agtggctaat gcgagtattc gtacgcctga taataagatc
      181 atattagaga acttgaactt tcatgtttcg ccaggcaaat ggctattact gaaaggctac
      241 tctggtgcgg gaaaaaccac actgcttaaa acattatccc actgctggcc gtggtttaaa
      301 ggtgatattt cttctcctgc tgacagttgg tatgtgtcac aaacaccgtt aatcaaaagc
      361 ggcttactga aagagattat ttgtaaagca cttcccctgc ccgtagacga taaatcgttg
      421 agcgaagtac tgcatcaggt tggtctgggg aaattggctg cgcgtattca tgaccacgat
      481 cgctggggag atattctttc cagcggcgaa aaacaacgta tcgccctggc acgattaatt
      541 ttacgacgtc cgaaatggat atttcttgac gaaactacct ctcatcttga ggaacaagag
      601 gctatacgct tactgcgttt agtgcgtgaa aaactaccca caagcggcgt cattatggtt
      661 acacatcaac ccggtgtctg gaacctggct gatgatattt gtgacattag cacggtttta
      721 taacaacaat aaaagccctg ctgta
//
LOCUS       DAETQN010000372          744 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.381, whole genome shotgun sequence.
ACCESSION   DAETQN010000372 DAETQN010000000
VERSION     DAETQN010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.381"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..397)
                     /locus_tag="K0W68_004756"
     CDS             complement(<1..397)
                     /locus_tag="K0W68_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBI3076036.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          233 a          173 c          108 g          230 t
ORIGIN      
        1 ccaacctgga ccatgctaaa ttcacaccgt gctgcgcggc ctgattaaat agtgcatacg
       61 ctttggcata atccacccgc acctgctcac cgtcgtagta gatacccccc agcgctaacg
      121 gagcttctct gtcaccttgt ttagccgcta actcgaacca tttaatcgct tcctgaacat
      181 caggtttggt atcataacga ggctgataat attcccagcc taacaggctc tgcgcgtcga
      241 tatctcccga ttcagccatt ttatgcagtg tatttataac atcgggagca ggtttaccac
      301 cgtatatgct gtcatacaat tcatgcgctt tctcgctggc caccacgctt ggtgaaacta
      361 tcagcattac agaaaataat attcttttag ttaacatgta tacctttatg tcctcactgt
      421 catatatttc cataataatt aaccaccaaa tatccattta atgaataaat acagtgccat
      481 aaaggtccaa gtaataagca cacatttagc ctgaaacatt atcattcatg tcaccaaatc
      541 gtttatttag tgagataaaa ctagcatatt cgtaagataa aaaatgcaca tggcaatcaa
      601 gaaatgatat tattactttt ttgatgtaaa actatttagt tttaaaattc attttttcaa
      661 catatagagc attcatttta tgaatgcaaa aaaacatacc tgacacacaa attactctcc
      721 tctgtaatat tttctcatta acac
//
LOCUS       DAETQN010000373          731 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.382, whole genome shotgun sequence.
ACCESSION   DAETQN010000373 DAETQN010000000
VERSION     DAETQN010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.382"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            81..239
                     /locus_tag="K0W68_004757"
                     /pseudo
     CDS             81..239
                     /locus_tag="K0W68_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
     gene            344..535
                     /locus_tag="K0W68_004758"
     CDS             344..535
                     /locus_tag="K0W68_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBI3076037.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(536..>731)
                     /locus_tag="K0W68_004759"
     CDS             complement(536..>731)
                     /locus_tag="K0W68_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBI3076038.1"
                     /translation="DVDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGN
                     LHYIDTGAVFGGELTLVQLQ"
BASE COUNT          189 a          185 c          161 g          196 t
ORIGIN      
        1 cagctgatag aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag
       61 taagttggca gcatcacccg ttaaccattc cttgtaaatc tgaccccgat ttatgtatgc
      121 aactcgatgc ctgggatgct gaaaccagta tccctgccct gctaaatggc gaacactctg
      181 tcctttaccg tacccgttac gatcaacaat ctgatgcctg gattatgcgt cttgcctgat
      241 ccaaaaagaa cccgtcggga tggcgggtta tttgtcctgg ttattccccc gttgtaaaat
      301 ctctcctaaa cttaacggta cggcaccaca cttcggggat gaaatgttcg cgctggtact
      361 ttttgtttgc tacctggatg gcggttgtga agatattgtt gtggatgtct acaacacgga
      421 acagcagtgt ctttattcta tgagcgatca acggatccgc catggcggtt gttttccgat
      481 tgaggatttt atagatggtt tctggcgacc tgcacaggag tacggtgatt tttaattatt
      541 gcaattgcac aagagtcagt tcgcccccaa agacagcacc ggtatcaata taatgcaggt
      601 tgccaatatc cacgcgatgt cgcaacggtg tatgaccaaa ccagaaatga tcagcacctg
      661 taattccctg ccctttttgg cgttcaccta atcgcgagcg gctccacaag acctgatgca
      721 aatcaacgtc c
//
LOCUS       DAETQN010000374          731 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.383, whole genome shotgun sequence.
ACCESSION   DAETQN010000374 DAETQN010000000
VERSION     DAETQN010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.383"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>731
                     /locus_tag="K0W68_004760"
     rRNA            <1..>731
                     /locus_tag="K0W68_004760"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          197 a          165 c          220 g          149 t
ORIGIN      
        1 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
       61 gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc tgaagcaaca
      121 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
      181 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
      241 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg aagtcccttg
      301 cggatggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
      361 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
      421 taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt
      481 aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat
      541 ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa
      601 gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac
      661 tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct
      721 gcatggagcc g
//
LOCUS       DAETQN010000375          730 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.384, whole genome shotgun sequence.
ACCESSION   DAETQN010000375 DAETQN010000000
VERSION     DAETQN010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.384"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..>730)
                     /locus_tag="K0W68_004761"
     CDS             complement(<1..>730)
                     /locus_tag="K0W68_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HBI3076039.1"
                     /translation="KAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFG
                     HNGVISWGSTAGFGDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAETF
                     TVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQA
                     LTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNP
                     LSGYIANWNNSPQKDYPASDLFAF"
BASE COUNT          194 a          194 c          155 g          187 t
ORIGIN      
        1 aaaaaggcaa acagatccga agcgggataa tctttttggg gagaattgtt ccagttagca
       61 atatatcccg acagggggtt atacacctta gggttcattt caaaaggcaa tagccctttc
      121 cagtcccatt ttcccgtacc aggaacgggt aatcgcggat catggcctga ttgacgatct
      181 ggataagcac cagtatgaac ataaccaata ttgccgttta catcagcata gtaccagttg
      241 atggtcagtg cttgtttcgc tgcctgctgt gtccactcct gccaattttt ggccttcatc
      301 tgatgagtcc aggccagcaa agacgccacc tctttaccat cccatgcgcg ggatttagcg
      361 taagccgttt gtgtcgtctg gtcagtttgg agaatgttgc catgcaccgt acgccagaca
      421 gtaaaggtct ctgcctgacc atttttcacc gtaatggttt cctcacggct taacattttc
      481 acccacttac cattatgcaa gtagtagcct ggtttctctg ccaacagccg ttcagcaaaa
      541 atatcgacat catcgccgaa acctgccgtt gatccccagg aaatcacacc attatgacca
      601 aaaaccagcc caggataggc aaatggtgta ttgccagtga catcataacc agcaccgtgc
      661 agaccaatac cataagtata cgcaggcgca taccagccaa actgcggacc atttaccatg
      721 attgctttcg
//
LOCUS       DAETQN010000376          730 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.385, whole genome shotgun sequence.
ACCESSION   DAETQN010000376 DAETQN010000000
VERSION     DAETQN010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.385"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(162..>730)
                     /locus_tag="K0W68_004762"
     CDS             complement(162..>730)
                     /locus_tag="K0W68_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3076040.1"
                     /translation="FPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVI
                     AGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFI
                     AVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQI
                     HLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          225 a          180 c          158 g          167 t
ORIGIN      
        1 gctttggctt gataaccgga taacaacgtg cgctttgttg atgccggatg cggcgtgaat
       61 tccttatccg gtctacaaaa tttgcaaatc caaagcatta cattaagacg tggcctgata
      121 aacatatcgc atcaggccat tttcgttagc tattgtccac gtcaggcttt cacatcactc
      181 actgcaccga ccggaatatc agcatgaaca ggctcgggcg gtactttgcg atatttatcc
      241 agcagatgaa tctggatcct gcgcagcgtg tcgccattga ggcgatagaa acggaagtag
      301 agaatcagcg ttaccatgaa aaacagagtt ggtagagcaa tcataataaa ctgcatacct
      361 aatagggctt gcgtagactg ttcaacattc ggtacatagc caatcatccc taacacaacc
      421 gcaatgaaaa aagccgcaaa ggctgaaccg cccttcacca ccatagtctg cacggagtaa
      481 gcgatacttt cacagcgtac gtgcagttta tattcaccgt aatcaacggt atctgccacc
      541 atgatgacct gtaataccca gaaaagcgcc gttcccacat tcagcaaaat acccgcaatc
      601 acaatgagga cgacgttgtg atagctcatt aatgccatta acaggagaac accacagctt
      661 aacaccggaa gaatagatgc tccggcccat aaaatgcgtc gggataatga tttaactaag
      721 cgtgggaaga
//
LOCUS       DAETQN010000377          726 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.386, whole genome shotgun sequence.
ACCESSION   DAETQN010000377 DAETQN010000000
VERSION     DAETQN010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.386"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            35..726
                     /locus_tag="K0W68_004763"
                     /pseudo
     CDS             35..726
                     /locus_tag="K0W68_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAETQN010000378          710 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.387, whole genome shotgun sequence.
ACCESSION   DAETQN010000378 DAETQN010000000
VERSION     DAETQN010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.387"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
BASE COUNT          245 a          109 c          147 g          209 t
ORIGIN      
        1 gatcgattca gatgccattt tgcagtagtc tattttataa aaaaattaag taggctaaga
       61 agtaccttag aaatcaattt taaggggtct aaaacgctct gtaacgagct aaactgtata
      121 agtggcagta agatgccttt tagactgaca ctcgcttaga gagcttccta gagcttctgg
      181 gaggattcat tagatatcgt tgtatacaga gacacagtat ctataaaggg tattaagaga
      241 ttcttagaag ttactgtaaa gaaccactaa gataagaaga gagggacaaa gaaccgaagg
      301 aaagtccctt attaagtagg agtcttaata gactgtctta atagataact taaaagtctt
      361 aaaggatatc ttccaagaag agaggaacga agaaccagag gaagtgaccg ttgaccgttt
      421 gaacagactg tttaagtaaa gcctataaag tatctattaa ttaagggcta tgtctcaata
      481 ctagcagttt gtcaatacct ttgtctttag caattcgtgc tatttattaa atatagcact
      541 ttttgttaaa acagagtatg ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca
      601 tgttcttggg ttcttagtga gagttacact atcttctaag gtactcaata aaaagacgag
      661 taacaaccga agaacgagga tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAETQN010000379          695 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.388, whole genome shotgun sequence.
ACCESSION   DAETQN010000379 DAETQN010000000
VERSION     DAETQN010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.388"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>695
                     /gene="tuf"
                     /locus_tag="K0W68_004764"
     CDS             <1..>695
                     /gene="tuf"
                     /locus_tag="K0W68_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBI3076041.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAETQN010000380          683 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.389, whole genome shotgun sequence.
ACCESSION   DAETQN010000380 DAETQN010000000
VERSION     DAETQN010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.389"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..560
                     /locus_tag="K0W68_004765"
     CDS             <1..560
                     /locus_tag="K0W68_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBI3076042.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
BASE COUNT          244 a          121 c          165 g          153 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acg
//
LOCUS       DAETQN010000381          672 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.390, whole genome shotgun sequence.
ACCESSION   DAETQN010000381 DAETQN010000000
VERSION     DAETQN010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.390"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            114..>672
                     /locus_tag="K0W68_004766"
     CDS             114..>672
                     /locus_tag="K0W68_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HBI3076043.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRL"
BASE COUNT          184 a          152 c          190 g          146 t
ORIGIN      
        1 gcagtatgat ggttaaaagg gtgaaactgg caggtgtgga gcctcgcaga tttccggcat
       61 catagtgccc aatacgggat gcataaagct ccctcagact ggagactcca caaatgaaat
      121 atacaccggt tggcgttgat atcgcaaaac atgtcattca gattcacttc atcaatgagc
      181 acacaggtga agtggttgat aaacagttgc gtagacagga ttttctgacg ttcttcggca
      241 accgtgagcc atgcctgatt ggtatggagg cctgtggagg ttctcagcac tgggcacggg
      301 aactgacaaa acttggtcat aaagtccggt tgttgcaggc ccgcttcgtt aaggcattcg
      361 tcatgggcaa taagaatgat gtgatggatg cccgggctat ctggatggcg gttcagcagc
      421 cgggtaaaga aatcgccgta aaaacagaag aacagcagtc ggtactggtt ctgcaccgta
      481 cccgcatgca actggtgaag ttccggaccg cacaaattaa tgccctgcac gggacgttac
      541 tggagtttgg tgaaaccatc cacaaaggcc gggcagcgat ggagcgggag ttccccgaag
      601 cactggaacg gatgaaagag agactgccac cgtatctcat tatggttctg gaaaaccagt
      661 acaaccgact ga
//
LOCUS       DAETQN010000382          643 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.391, whole genome shotgun sequence.
ACCESSION   DAETQN010000382 DAETQN010000000
VERSION     DAETQN010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.391"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>643
                     /locus_tag="K0W68_004767"
     CDS             <1..>643
                     /locus_tag="K0W68_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3076044.1"
                     /translation="EQWRQQFPVEAMCRVPGVSRSGYYDRVQHVPSDRKQSDERLKLE
                     IKVAHIRTRETYGTRRLQTELADNGIIVGRDRLARLRKELRLHCKQKRKFRATTNSDH
                     NLPVTPNLLNQNFTPTAPNQVWVADITYVATREGWLYLAGVKDVYTCEIVGYAMGERM
                     TKELTGKALFMALRSQRPPAGLIHHTDRGSQYCAYDYRVIQEQSGLKTSMSRKG"
BASE COUNT          173 a          163 c          179 g          128 t
ORIGIN      
        1 tcgaacaatg gcgacaacaa ttccctgttg aagcgatgtg tcgggtacct ggtgtatcca
       61 ggagcggtta ttacgaccgg gtacagcatg tcccctcaga cagaaaacaa agtgatgagc
      121 ggctaaaact ggagattaag gtggcacata tccgcactcg cgaaacatat ggaacccggc
      181 ggctccagac ggagctggca gataacggaa ttatcgtcgg tcgtgacaga ctggcacgtc
      241 ttcgtaagga gctgaggctg cactgtaagc agaaacgcaa gttcagggcg accacaaact
      301 ctgaccacaa tctgccggtt acgccgaatc tgctgaatca gaactttacg cccacagcac
      361 ctaatcaggt ctgggtcgca gatataacgt atgttgccac ccgggaagga tggctgtatc
      421 tcgccggcgt caaagatgtt tatacgtgcg aaattgtcgg ttacgccatg ggagagcgca
      481 tgacgaaaga gttgacgggt aaagccctgt ttatggcgct caggagccag cgcccacctg
      541 ccgggctaat ccaccacacc gatcgaggtt cacagtactg cgcatacgat taccgggtca
      601 tacaggagca gtctggtctg aaaacatcaa tgtcgcgtaa agg
//
LOCUS       DAETQN010000383          641 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.392, whole genome shotgun sequence.
ACCESSION   DAETQN010000383 DAETQN010000000
VERSION     DAETQN010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.392"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>641
                     /gene="fliD"
                     /locus_tag="K0W68_004768"
     CDS             <1..>641
                     /gene="fliD"
                     /locus_tag="K0W68_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBI3076045.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          217 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac a
//
LOCUS       DAETQN010000384          641 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.393, whole genome shotgun sequence.
ACCESSION   DAETQN010000384 DAETQN010000000
VERSION     DAETQN010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.393"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>641
                     /gene="istA"
                     /locus_tag="K0W68_004769"
     CDS             <1..>641
                     /gene="istA"
                     /locus_tag="K0W68_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HBI3076046.1"
                     /translation="LKFDKKLTNRCIGLTLHISASTVFEVLARFKASSLSWPLPADIS
                     HDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQAGDKAMGYSH
                     FCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVAIFVAVMGAS
                     NYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRYEPVI"
BASE COUNT          156 a          173 c          163 g          149 t
ORIGIN      
        1 gctcaagttc gataagaagc tgactaaccg ttgtatcgga ctgacgttac acatcagcgc
       61 ctcaaccgtt tttgaggttc ttgcccgctt caaagcctct tctctgtcat ggccattacc
      121 tgcggatata tcccatgaca cgctggagaa actcattttc ccgcccaaag acacctctgc
      181 gtcagagctt gttatgcctg acatgctgta cttcgatacg gaaatgagaa aacctggcgt
      241 gacgcgccaa ctgttgtgga tggaatacaa ggcacaggcg ggtgacaaag cgatgggata
      301 ctcacacttc tgccgttgct accggaagtg gaaaaaaacg cgacgcctct ccatgcgaca
      361 ggagcatcgt gctggcgaaa agctctttat cgacttctgt ggtcctacgg tccctgttat
      421 taatccggac accggagaga tacgccgcgt ggcaatcttc gtggctgtaa tgggggcgtc
      481 caactatacc tatgttgaag cgtgcgaagg tcaggacatg atgtcatggt taaatgccca
      541 cagccgttgc ctgaccttcc tgggcggcgt accgaagctg ctcatccccg ataacctcag
      601 aagcgcagtg aaaaaagccg atcgctatga gcctgtcatc a
//
LOCUS       DAETQN010000385          639 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.394, whole genome shotgun sequence.
ACCESSION   DAETQN010000385 DAETQN010000000
VERSION     DAETQN010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.394"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(19..369)
                     /gene="traA"
                     /locus_tag="K0W68_004770"
                     /pseudo
     CDS             complement(19..369)
                     /gene="traA"
                     /locus_tag="K0W68_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            complement(415..>639)
                     /locus_tag="K0W68_004771"
     CDS             complement(415..>639)
                     /locus_tag="K0W68_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3076047.1"
                     /translation="DSYDNAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT          164 a          173 c          137 g          165 t
ORIGIN      
        1 acatcgtttt atttcctgtc agaatccggc aacagacata cctactgcaa taaatacaga
       61 gagtatggca aagccgacca ggaacttgac gtttttggtc atcatgtaca tgactgcacc
      121 gaccagaact tcagccagaa caacccattt aacgatactg gagtccttac cgaaggtcgc
      181 cttaaccgtt tcctgacctt tagccatcag gtccgtgcct ttaccagcag ccatagctaa
      241 ctcagggaac aacatcatca gaacagcaac cgggatcact gcacgaacca ggcgaagcat
      301 attcagacga gtgaatttag aaaaaaacga tttctttttg acgggcgcag aagtaccctg
      361 aacacttaac gctgaaccgc cccgggtttc ctggagagtg ttttatctgt gaactcaggc
      421 tgccagatca tcgtttccga tggaagcata ataagctttt tctgcttctg ccggaggagt
      481 atggcccagc ctttccagca atcgtcgatt gttataccag tccacccacg tgagtgtggc
      541 cagctccact tctgtccggt ttttccagct cttacggtgt attacctccg ctttgtaaag
      601 gcccttgatg atctctgcca tcgcgttgtc atacgagtc
//
LOCUS       DAETQN010000386          597 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.395, whole genome shotgun sequence.
ACCESSION   DAETQN010000386 DAETQN010000000
VERSION     DAETQN010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.395"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(76..423)
                     /gene="tnpB"
                     /locus_tag="K0W68_004772"
     CDS             complement(76..423)
                     /gene="tnpB"
                     /locus_tag="K0W68_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBI3076048.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          130 a          162 c          171 g          134 t
ORIGIN      
        1 atgtcgtcag aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa
       61 ccaggataaa gagatttaca gcatggtcag ggaggtcagc agccgcttag gctgtcgcca
      121 gtcgatacct tccagcagca tcgccagctg cgcctgtgta aggaacactt tgccatcacg
      181 ggctgacggc caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc
      241 actggtggac cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaacatg
      301 gccggacatg ggatcgtctt tcagcgccgt ctgtactttc gcagccaggc cgttgaagcc
      361 atttctcata tcggtgatac cggcaaccag ccaaattttg atcccggaag gtaacgggat
      421 catcgcttca gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag
      481 cggagcgtcc cgtgccggaa cgttacctca cagctgatac tgagggtttt cgggtcctct
      541 gcgagcgatt ctggctgttc ggcagctgca tcgagagtca caggaagtag ctggggg
//
LOCUS       DAETQN010000387          597 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.396, whole genome shotgun sequence.
ACCESSION   DAETQN010000387 DAETQN010000000
VERSION     DAETQN010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.396"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..290
                     /locus_tag="K0W68_004773"
     CDS             <1..290
                     /locus_tag="K0W68_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3076049.1"
                     /translation="LVTDTITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNM
                     TLRLRLQDQQEETSVTLFQGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
BASE COUNT          177 a          145 c          123 g          152 t
ORIGIN      
        1 tgctggtcac tgacaccata acagaggcca ccctgtacga aaacgggctg tatacgcgcc
       61 acatcagact cagtcgccca gtatctgaca gcaaataccc cttgccaccc ggatctcagc
      121 tcattaaaaa tatgacctta cgactgcgcc tgcaggatca gcaggaagaa acatccgtga
      181 cattatttca gggaaaaatg agtaccgacg gaaataacta ttacatcatc aaaggtaaag
      241 taagctcagt acttctgtta ttaaaaatgc tgcagattaa tcacgcctga tctctgtccc
      301 ggtattttag cccgaggtat attctgaaat atcattcatc atgaccggcg tttatacaaa
      361 cgccggtcat tcgctaacag cttcaccgtt ccttccccca tccaggttcc gcatgagcgt
      421 gttgtaatgt tcataagcgt attatcagga ttgtctggaa aattcccgat actttggact
      481 attactcctg aacaattcga aattcagcag attaaaggta agcgttaacg gaggtaagcg
      541 ttaacggagg taagcgtaaa ccgactgccg tatgtagcca ttaagccagt attggta
//
LOCUS       DAETQN010000388          593 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.397, whole genome shotgun sequence.
ACCESSION   DAETQN010000388 DAETQN010000000
VERSION     DAETQN010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.397"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            289..>593
                     /gene="mcbR"
                     /locus_tag="K0W68_004774"
     CDS             289..>593
                     /gene="mcbR"
                     /locus_tag="K0W68_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBI3076050.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          172 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tga
//
LOCUS       DAETQN010000389          575 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.398, whole genome shotgun sequence.
ACCESSION   DAETQN010000389 DAETQN010000000
VERSION     DAETQN010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.398"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
BASE COUNT          157 a          113 c           91 g          214 t
ORIGIN      
        1 tatttatact tatcgggtat cagttttgat aggggtatca gacttgatag gggtaacaga
       61 attctgtggg ggtagttcac tatgacctac ccctagttca ctgtgagcta ccgttacatc
      121 caatcatctt ccgatgtaac gtatcgttac taccgatgtg taatatcgtt acattcaata
      181 attagtctat gtaatatatc gttactacca actttcttac agagagcatt tcagataaat
      241 gaatgtaacc tctcgttaca tccatttgtc acatagcact ctgtgtaatg tctttctatg
      301 tgtgtgccat aatcccccta tattccctgt gtaaatcata gaataatgct ctctattccc
      361 tgtgtaattc cctactaaaa aatccaatga aaagtagctt aaaagttcaa ttcttacttt
      421 cttcactttt acatcacttt cgcaaagcct tgtgtaatgc ggcttacaga gcaccatcag
      481 aaattgttgg ctaaaaattg ttctgtttgt ttctgaacga gtttctcgtt tgtgcaaatt
      541 ttaaaattta ttttgttttt atagccttat ttcgt
//
LOCUS       DAETQN010000390          574 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.399, whole genome shotgun sequence.
ACCESSION   DAETQN010000390 DAETQN010000000
VERSION     DAETQN010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.399"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..516)
                     /locus_tag="K0W68_004775"
     CDS             complement(<1..516)
                     /locus_tag="K0W68_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBI3076051.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDSK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNP"
BASE COUNT          160 a          137 c          115 g          162 t
ORIGIN      
        1 tgggttgctg gcatcattgc ctttcgcatc acccggacag ttagaagagg ctacccacca
       61 agcattttta tataccgcac cattaaagac gacataccag gtctgaccgc cctgctgttt
      121 attccaggca actacagaag aattagacgg aacagtgccc tgtgctggtg tagccgaacc
      181 gccattagcc ggcgtattgg atgcaggcgt atttgaattt gaagaagatg agctgccgct
      241 cttttcgcag gaaagtgtat tgccaaactg actaatttca gcagctgttg cggtacgctt
      301 taaacgccac gggttagttg catcatttgc tttcgctttt cccgggcaat ttgtagaaaa
      361 accccaccag gcattttcat aaattttgct atcgaaaata acctgatatt tattaccacc
      421 ttgttggtta ttccatgctt ccattgccaa tgctggcaga gcggaacaca ccagccccat
      481 accaatcata gatttagtaa atatatttaa tttcattata atcccttgtg acgtaaaaac
      541 tgcaaaacaa aagtcctacg caggaattat ttac
//
LOCUS       DAETQN010000391          569 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.400, whole genome shotgun sequence.
ACCESSION   DAETQN010000391 DAETQN010000000
VERSION     DAETQN010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.400"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(409..>569)
                     /locus_tag="K0W68_004776"
     CDS             complement(409..>569)
                     /locus_tag="K0W68_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HBI3076052.1"
                     /translation="YFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGA
                     ILGKRFEI"
BASE COUNT          149 a          125 c          112 g          183 t
ORIGIN      
        1 tgattttcag gaaccgtttt gacggcttcg gctttaaact ccgtagagta acgttgggtt
       61 tttctgctca ttattggctc cttctgatgc cattctattt caggaaggag tgtccgttaa
      121 actcaggcta cctcactccc gtaaagacgg gagtcaagtt cccgctccga cataactgcg
      181 tgcaatggat tttgaaggaa ccgtcagccc attccccgga ggcaaacatg ggctggcaaa
      241 agtgtagcgg tattaggcac agctatttag gaaagaggga ccatctccat ctcattaaat
      301 ggaccacgcc agacaattca ctgtcatgct gatgctgcac tgtggggaca gaatcactca
      361 tgatgacttc gataatcgac gacattatta tttggccgag cttttaggtc atatctcaaa
      421 tcgtttgcct aatattgctc cattttttaa taaaaaatca atcatttttg aatctttgct
      481 tttcattgca ttgtcaagca tagtgtcccc actatttgct ttttgaaatt ttttgtttac
      541 atcaaccaat ccattattga taaaatatt
//
LOCUS       DAETQN010000392          559 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.401, whole genome shotgun sequence.
ACCESSION   DAETQN010000392 DAETQN010000000
VERSION     DAETQN010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.401"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..428
                     /locus_tag="K0W68_004777"
     CDS             <1..428
                     /locus_tag="K0W68_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HBI3076053.1"
                     /translation="QLTSVARQNETCKRLLDIPGVGPLIATAAVATMGEASAFKSGRE
                     FAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTLFIHGARAVALVAKEPGPWITELKK
                     RRPASVAIVAMANKLARTVWAITAHDRKYDRNHVSIRPY"
BASE COUNT          163 a          124 c          156 g          116 t
ORIGIN      
        1 aacagcttac cagcgtggcg aggcagaatg aaacctgtaa gcggttgctg gatattcctg
       61 gcgttggacc acttattgcg acggcagcgg tggccaccat gggggaagca tcagcgttta
      121 aatcggggcg agagttcgcc gcatatgttg gtctggttcc aaaacaaact ggctccggag
      181 ggaaagtacg tctgctgggg ataagcaaac gtggtgacac ttatctcagg acattattta
      241 tccacggtgc aagagcggtg gcattagtag ctaaagagcc tggcccgtgg ataaccgaac
      301 tgaaaaaacg tcgtccagcc agtgtggcaa tcgtcgccat ggcaaacaag ctggcacgaa
      361 cagtatgggc gataaccgcc catgaccgta agtatgacag gaaccacgtc agtatcagac
      421 catattaatc gctgatacca ttaaacaatg aactcttaac aaaagggtga atgctgaaag
      481 gttgctatgg cggccagagt gatgacaaag acaggtaaga ccgtgactca ctaaacctga
      541 acagtatttt gggcttgaa
//
LOCUS       DAETQN010000393          559 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.402, whole genome shotgun sequence.
ACCESSION   DAETQN010000393 DAETQN010000000
VERSION     DAETQN010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.402"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>559
                     /locus_tag="K0W68_004778"
     CDS             <1..>559
                     /locus_tag="K0W68_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBI3076054.1"
                     /translation="ITQVVISKYTDHLPLYRQQHIFSRMGVELPVSTMADMVGVAGAA
                     LAPLVKLLRHELLTRDVIHADETSLRLLDTRKGGKSCSGWLCAYVSGERSGPPVVCFD
                     SQTGRALRYPETWLQCWCGGTLVSDGYSVYKSLADNHPGITSACCWSHAGRGFANLYK
                     ASREPRAGVELRKIAGLYRIEKLIRE"
BASE COUNT          116 a          154 c          167 g          122 t
ORIGIN      
        1 cattacccag gttgtcatca gcaaatatac cgaccatctg cccctgtatc gccagcagca
       61 cattttctcc cgaatgggtg tggagctccc cgtcagtaca atggcggata tggtcggtgt
      121 tgccggagct gcactggccc cgctggtgaa actgctacgc catgagcttc tgacacggga
      181 tgttattcat gcagatgaaa cgtccctgag gctccttgat acccggaaag gcggtaaatc
      241 ctgctccggc tggctgtgtg cttacgtcag cggtgaaaga agcggtccgc cggtcgtctg
      301 ttttgactcg cagaccggac gggccctcag ataccctgag acctggctgc agtgctggtg
      361 tggcggtacg ctggtgagtg acggatacag cgtgtacaaa tctctggcgg ataatcaccc
      421 cggcatcaca tcagcctgtt gctggagtca cgcagggcgc ggcttcgcaa atctgtataa
      481 ggccagcagg gagccccgtg caggcgtgga gctgagaaaa atcgcgggtc tttaccgcat
      541 tgagaaactc atccgtgag
//
LOCUS       DAETQN010000394          559 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.403, whole genome shotgun sequence.
ACCESSION   DAETQN010000394 DAETQN010000000
VERSION     DAETQN010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.403"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            109..>559
                     /locus_tag="K0W68_004779"
     CDS             109..>559
                     /locus_tag="K0W68_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HBI3076055.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLK"
BASE COUNT          135 a          132 c          149 g          143 t
ORIGIN      
        1 gagacagatg ctggccaacg tgggatattt aacgcgggtt aacatcgttg tcagagccgc
       61 atgggtgagc tgttgctaca ccccggtatt tccaccacgg aggccagcat gaacaacaat
      121 aacacattgt acgtcgggtt agatgttcac aaagaatcga ttactgtcgc ttatgctatc
      181 aattcagaac ctgttgaact gatgggtaaa attggcacat cacctactga tattcagaat
      241 ctttgtaaac gtctcaggtc aaagtcatcg caggtcagta tcgtttatga agcggggccc
      301 tgtggttatg gactttatcg tcggctggtg aaatccggtt ttgactgcat ggtctgcgcc
      361 ccctcactta tcccgaaaaa accgggggag cgggttaaaa ccgatcgccg tgatgccatc
      421 agacttgtgc gttcactgcg tgcaggagat ctttctgctg tctacgtacc cggcattgaa
      481 gatgaggcat tccgggattt ggcccgggca tgggcatctg cccgcgatga tttacggcat
      541 gcaaggcaac gtctgaaat
//
LOCUS       DAETQN010000395          557 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.404, whole genome shotgun sequence.
ACCESSION   DAETQN010000395 DAETQN010000000
VERSION     DAETQN010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.404"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>557
                     /locus_tag="K0W68_004780"
     CDS             <1..>557
                     /locus_tag="K0W68_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBI3076056.1"
                     /translation="IPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRALNARL
                     RELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPDYHVEY
                     ARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHMPERHR
                     RHGTWTPERLIEQGNRTGPSTGRVV"
BASE COUNT          142 a          169 c          148 g           98 t
ORIGIN      
        1 tatcccggcc cgcccgcgca aacctaaaga taaaccaaaa gcagaaaacg gcgtcctcat
       61 cgtcgagcga tggcttcttg cccgcatccg taatgaaacc ttccacacgc tcagggcgct
      121 gaatgcacgc cttcgggagt tgctgacgga catgaataac cgaccaatga agggatacgg
      181 taatcaaacc cgcgcagaac gcttccgcat gctggatgca ccagcactct ctccgctgcc
      241 tctggagcct tatgaataca cggaatacaa agccgtgaag gttggccctg actaccacgt
      301 tgaatatgcc cgtcactggt actccgttcc acacgaactg gtcggtcagc gtctgtcact
      361 gaaggtgggg cagtcggtgg tacagctctg gcacaagggg cagtgtgtgg cgcaacatcc
      421 gcgcagcaca catgaatata aacacacaac caatccgctt cacatgccag aacgccaccg
      481 gcggcatggg acatggacac cggaacgcct tatcgagcag ggaaaccgta caggtccgtc
      541 cacaggccgg gtggttg
//
LOCUS       DAETQN010000396          552 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.405, whole genome shotgun sequence.
ACCESSION   DAETQN010000396 DAETQN010000000
VERSION     DAETQN010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.405"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..552
                     /locus_tag="K0W68_004781"
                     /pseudo
     CDS             1..552
                     /locus_tag="K0W68_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT          196 a          120 c           84 g          152 t
ORIGIN      
        1 gattatccga cttattaaaa atgcctgttt aaaattatat aatttagcac ataccgcaac
       61 caactcccac cttaaaccaa ctggtccgga taattcagat gttctcctgt tcaaaaagct
      121 attctcccct tcgaaattaa tgacaattat cggtgatgaa attcctctta tatctgaaaa
      181 acagtcgctt tcaaaggtgc ttttaaatga tgagaataat gaactgagtg atggtacaaa
      241 cttctgggat aaaaatcgtc aattaaccac agatgaaata gcttgctatc ttcagaagat
      301 cgccgctaat gcaaaaaata ctcaagtcaa ttatcctact ggtctctacg tcccctactc
      361 caccagaact cacctggaag acgctctcaa tgaaaatatt aagagcgatc catcatggcc
      421 gaatgaagtc cagttattcc ccataaatac tggcggacac tggatattag tttcgctaca
      481 gaaaatagta aataaaaaaa taataaacta caaataaaat gcgtcatatt caactcattg
      541 cgtgcactag gc
//
LOCUS       DAETQN010000397          550 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.406, whole genome shotgun sequence.
ACCESSION   DAETQN010000397 DAETQN010000000
VERSION     DAETQN010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.406"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>550
                     /gene="feaR"
                     /locus_tag="K0W68_004782"
     CDS             <1..>550
                     /gene="feaR"
                     /locus_tag="K0W68_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HBI3076057.1"
                     /translation="QEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDASR
                     PCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNNP
                     ALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWIA
                     GETGMSVRSLYRMFADKGLVVA"
BASE COUNT          139 a          125 c          146 g          140 t
ORIGIN      
        1 ggcaggaagt aaaaggcagc gacgatgcct ggttttacac cgtttttcag cttagtggtc
       61 aggcaataat ggagcaggat gagcgtcagg tgcagattgg cgctggcgat attacgttac
      121 tcgatgcctc acgcccctgt tcgctttact ggcaggagtc ttctaaacag atttcattac
      181 ttttgccacg cactctgctg gaacaatatt ttccccatca aaaacctgtc tgcgcagaaa
      241 gactggacgc tgacttaccc atggtgcaac tcagtcatcg cctgttacag gagagcatga
      301 ataatccggc actttctgaa acagaaagtg aagctgcgct acaggcgatg gtgtgtctgc
      361 tgcgcccggt acttcatcag cgggaatctg ttcaacctcg ccgtgaacgt cagtttcaaa
      421 aagtggttac gttgatagac gataatattc gcgaagagat attacgcccg gagtggatag
      481 ccggagagac aggtatgtca gtacgtagtt tgtaccgaat gtttgccgat aaaggtttgg
      541 tagtcgcgca
//
LOCUS       DAETQN010000398          520 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.407, whole genome shotgun sequence.
ACCESSION   DAETQN010000398 DAETQN010000000
VERSION     DAETQN010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.407"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>520
                     /locus_tag="K0W68_004783"
     CDS             <1..>520
                     /locus_tag="K0W68_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI3076058.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLA"
BASE COUNT          138 a          121 c          117 g          144 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc
//
LOCUS       DAETQN010000399          509 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.408, whole genome shotgun sequence.
ACCESSION   DAETQN010000399 DAETQN010000000
VERSION     DAETQN010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.408"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..>509)
                     /gene="espL1"
                     /locus_tag="K0W68_004784"
     CDS             complement(<1..>509)
                     /gene="espL1"
                     /locus_tag="K0W68_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HBI3076059.1"
                     /translation="LDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALLRHPPA
                     GSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSVYGQLNYVV
                     SVYDPNDTNVAVRGTHRAARGFLSLDKFISSAPDAQTWADRYVRNCAIAILPLLPEGV
                     PVAILAGITT"
BASE COUNT          149 a          115 c          117 g          128 t
ORIGIN      
        1 gtcgtgatac ccgctaaaat agcaactgga actccctcag gtaatagagg aagaatagca
       61 attgcacagt tgcgaacata cctatcagcc caggtctgag catcgggagc tgaactgatg
      121 aacttatcaa gcgagagaaa gccccgtgct gccctgtggg tgcctctgac ggcaacgttg
      181 gtatcgttcg ggtcgtaaac agacacaaca taatttaatt gtccatacac tgactgcttt
      241 attcgtagcc ttactgacaa agtatgattg tctatatata aaatcgcgga catatgactg
      301 acattgtttg ccatcattcg acaaaagaaa atatctaaag cgaagccaaa atccttactc
      361 gcgataacgc gactccctgc aggaggatgg cgaagtaatg ctctgaactc cgcgtcccgg
      421 actaagggga catgctgctg tatggattca acggaggagt agtgtttaat caggttgact
      481 ttgccttttc catttctcaa tgaatcaag
//
LOCUS       DAETQN010000400          508 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.409, whole genome shotgun sequence.
ACCESSION   DAETQN010000400 DAETQN010000000
VERSION     DAETQN010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.409"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..188)
                     /locus_tag="K0W68_004785"
     CDS             complement(<1..188)
                     /locus_tag="K0W68_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3076060.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDG"
     gene            242..445
                     /locus_tag="K0W68_004786"
                     /pseudo
     CDS             242..445
                     /locus_tag="K0W68_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          126 a          136 c          118 g          128 t
ORIGIN      
        1 ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc ggacacgcag agtctccggc
       61 gtacagccaa tctttggggc aatggaacaa attgccgccc actgtgagtc atattcatcc
      121 tgactttcca gaaccatacg aatcgcccgc tgacggactt cgggggaaaa acgagtattt
      181 ttagtcatcc tgtttacctc tttctcaggg agtttagtct ccaggattcc cggggcggtt
      241 cagacatcta gaatttactg tcagcaccgg cgttaaagtt tacttctgcg atcctcagag
      301 tccttggcag cggggaacaa atgagaatac aaatgggcta attcggcagt actttcctaa
      361 aaagacatgt cttgcccaat atactcaaca tcgagtaggc agcctgacgg ctgcggcttg
      421 tcatggtctg caattaccgt tataaaaaca ggcaatgtca ttgtctttca ggtggttata
      481 tggcccgttc agctaaaccc cgtaaacg
//
LOCUS       DAETQN010000401          507 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.410, whole genome shotgun sequence.
ACCESSION   DAETQN010000401 DAETQN010000000
VERSION     DAETQN010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.410"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            227..>507
                     /locus_tag="K0W68_004787"
     CDS             227..>507
                     /locus_tag="K0W68_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3076061.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSL"
BASE COUNT          174 a           97 c          107 g          129 t
ORIGIN      
        1 aacgcttaaa gattttaacg atcgcttatt gtaaaaataa tagcaaagaa aattgtaaga
       61 tacagaccgt aaataaaaac atcttccttg gtcaggaaag aaatcttgaa agcatatctg
      121 gctacgacaa aatcgttgtc aaatataata actggatcac caaagataat ggcgagtttg
      181 aactggcggt ccattaatta tcattgtaat aaacgaagga atagtaatga aaaaggtatt
      241 tgcaaaatct cttctggtcg cagcgatgtt ttctgttgca ggctccgcat tggcagtaca
      301 aaaggatatt actgtaacgg ccaacgttga tgccgctctg gatatgacac agaccgataa
      361 caccgcgttg ccgaaagcag tagaaatgca atatctgccg gggcagggtc tgcaatctta
      421 ccagttgatg accaaaatct ggtctaacga cgttaccaaa gatgtaaaaa tgcagttagt
      481 ttccccggcg caactggttc aaagcct
//
LOCUS       DAETQN010000402          496 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.411, whole genome shotgun sequence.
ACCESSION   DAETQN010000402 DAETQN010000000
VERSION     DAETQN010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.411"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..159
                     /locus_tag="K0W68_004788"
     CDS             <1..159
                     /locus_tag="K0W68_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3076062.1"
                     /translation="RDAYCNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIR
                     LFYQRLRN"
     gene            298..453
                     /locus_tag="K0W68_004789"
                     /pseudo
     CDS             298..453
                     /locus_tag="K0W68_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          142 a          103 c          120 g          131 t
ORIGIN      
        1 cgtgatgcgt actgcaatcg taatgtcgtt gaacggtgct ttgggcggct gaaagaatat
       61 cgccgcatcg ccacgcgtta cgacaaaacg gcgagaaatt acctggcgat ggtgaaactg
      121 ggctgcatcc gactctttta tcaacgctta cgtaattaag ggacacagcc tggcactctg
      181 cgagtaaact tgctggtaaa ctattattga cagttggttt tgttttgaga ctgtaattta
      241 aattgtgtaa ttgcctgttt ttgatatgtt cactccaaca acggagacag gtaaattatg
      301 gacgaaaaga agcttaaagc ccttgcggct gaactggcta aaggcctcaa aaccgaggcc
      361 gacctcaatc agttttcccg tatgcagacg aaattaactg tcgaaacggt gcttaatgcg
      421 gaactaactg accatctcgg gcatgaaaaa agctacatcc ggtagtaaac attatcagtg
      481 ggttatgcga tatgat
//
LOCUS       DAETQN010000403          476 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.412, whole genome shotgun sequence.
ACCESSION   DAETQN010000403 DAETQN010000000
VERSION     DAETQN010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.412"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>476
                     /locus_tag="K0W68_004790"
     rRNA            <1..>476
                     /locus_tag="K0W68_004790"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAETQN010000404          473 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.413, whole genome shotgun sequence.
ACCESSION   DAETQN010000404 DAETQN010000000
VERSION     DAETQN010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.413"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..>473)
                     /locus_tag="K0W68_004791"
     CDS             complement(<1..>473)
                     /locus_tag="K0W68_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBI3076063.1"
                     /translation="VYTFILSVLIYREVKWRHLPKLILESVVTTSIVLLLIGFSVGMS
                     WAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMTPAVLIFTPIFLPIA
                     QELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQHLIKPLLPFYAS"
BASE COUNT          162 a          107 c           98 g          106 t
ORIGIN      
        1 tgctagcata aaatggcagc aatggtttga ttaaatgttg aatcttcaca cccgagatag
       61 aacaaccaac aaatagcgca ctacccaccg gcggtgttaa cagcccaata caaaggttgg
      121 ccaccatcat aataccgaaa tgtactgggt ccattcccag ctcttgtgca atcggcaaga
      181 agatcggtgt aaagattagt acagccggtg tcatgtccat aaagatgccg acaattaata
      241 agacgatatt aatcagcagc aatatgatca gtgggttatc agaaatcccc atcaatgcgt
      301 cgctgatcat ataaggaata tcagcattag tcattgccca ggacatgccc acggaaaaac
      361 cgatcaacag taatacaatc gaagtggtaa caaccgactc cagaatcagt ttgggtaaat
      421 ggcgccattt tacttcccgg taaatcagca ccgagagaat aaatgtgtag aca
//
LOCUS       DAETQN010000405          472 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.414, whole genome shotgun sequence.
ACCESSION   DAETQN010000405 DAETQN010000000
VERSION     DAETQN010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.414"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            89..451
                     /locus_tag="K0W68_004792"
     CDS             89..451
                     /locus_tag="K0W68_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBI3076064.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKD"
BASE COUNT          114 a          112 c          138 g          108 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt gggtacgtcc tgaaagactg aggtagcctg agtttaacgg ac
//
LOCUS       DAETQN010000406          471 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.415, whole genome shotgun sequence.
ACCESSION   DAETQN010000406 DAETQN010000000
VERSION     DAETQN010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.415"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..286
                     /gene="traL"
                     /locus_tag="K0W68_004793"
     CDS             <1..286
                     /gene="traL"
                     /locus_tag="K0W68_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HBI3076065.1"
                     /translation="KYRFPETLTNQSRWFGLPLDELIPAAICVGWGITTSKYLFGIGA
                     AVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWIK"
     gene            308..>471
                     /locus_tag="K0W68_004794"
     CDS             308..>471
                     /locus_tag="K0W68_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3076066.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGF"
BASE COUNT          113 a          106 c          115 g          137 t
ORIGIN      
        1 gaaatatcgt ttcccggaaa cactgaccaa ccagagccgc tggtttggcc tgccactgga
       61 tgaactgatc cccgcagcaa tctgtgttgg ctggggtatc acaacatcga aatatctgtt
      121 cggtattggt gcagcggttc tggtttattt cgggattaaa aaactgaaaa aagggcgggg
      181 cagttcctgg ttacgtgacc tgatttactg gtatatgcca acagccctgc tgcgcggtat
      241 ttttcataat gttcccgatt cgtgtttccg gcagtggatt aaatagaact gataccagga
      301 ttgttatatg gaacacggtg cccgtttaag taccagtcgt gtaatggcca tcgcctttat
      361 atttatgtca gtgcttattg ttctcagcct ctctgttaac ctcattcagg gggtgaataa
      421 ctaccgtctt cagaatgagc aacgcactgc cgtgacccca atgggattta a
//
LOCUS       DAETQN010000407          462 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.416, whole genome shotgun sequence.
ACCESSION   DAETQN010000407 DAETQN010000000
VERSION     DAETQN010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.416"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            163..363
                     /locus_tag="K0W68_004795"
                     /pseudo
     CDS             163..363
                     /locus_tag="K0W68_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          140 a          107 c          111 g          104 t
ORIGIN      
        1 actcacgtgg gtggactggt ataacaatcg acgattgctg gaaaggctgg gccatactcc
       61 tccggcagaa gcagaaaaag cttattatgc ttccatcgga aacgatgatc tggcagcctg
      121 agttcacaga taaaacactc tccaggaaac ccggggcggt tcaaccttca cgataaaaaa
      181 cttttcatta aaggtaaagc ggatcaatat ccggcacttc attctctgat atctccgacc
      241 cgcattaatc tgaaagaaat agaaattcac tggcgtgaag tgctacgtct ggcgacctca
      301 ataaaacagg gaacagtgac agcatccctg atgctgagaa aacttgccag ttaccccaaa
      361 caaaagtaag gcagtatgac tttccgtgag acagatgctg gccaacgtgg gatatttaac
      421 gcgggttaac atcgttgtca gagccgcatg ggtgagctgt tg
//
LOCUS       DAETQN010000408          461 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.417, whole genome shotgun sequence.
ACCESSION   DAETQN010000408 DAETQN010000000
VERSION     DAETQN010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.417"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>461
                     /locus_tag="K0W68_004796"
     CDS             <1..>461
                     /locus_tag="K0W68_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3076067.1"
                     /translation="LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTL
                     RSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQ
                     GASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          134 a           94 c          143 g           90 t
ORIGIN      
        1 tctgctgacg tcgatgacgg acgccatgcg cttccccgga ggagaaatgg cggatctgta
       61 cagtcatcgc tgggaaatcg aactgggata cagggagata aaacagacga tgcaactgag
      121 caggctgacg ctgagaagta aaaagccgga gcttgtggag caagagctgt ggggtgtctt
      181 actggcttat aatctggtga gatatcagat gattaaaatg gcggaacatc tgaaaggtta
      241 ctggccgaat caactgagtt tctcagaatc atgcggaatg gtgatgagaa tgctgatgac
      301 attgcagggc gcttcaccgg gacgtatacc ggagctgatg cgcgatcttg caagtatggg
      361 acaacttgtg aaattaccga cgagaaggga aagggccttc ccgagagtgg taaaggagag
      421 gccctggaaa taccccacag ccccgaaaaa gagccagtca g
//
LOCUS       DAETQN010000409          455 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.418, whole genome shotgun sequence.
ACCESSION   DAETQN010000409 DAETQN010000000
VERSION     DAETQN010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.418"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..386
                     /locus_tag="K0W68_004797"
     CDS             <1..386
                     /locus_tag="K0W68_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBI3076068.1"
                     /translation="KGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDVY
                     NSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNIIC
                     SECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          133 a           96 c          122 g          104 t
ORIGIN      
        1 ttaaagggca ggttgttgaa cttgtacgtc tgagcggtcg cgagacgttg cggcaactgg
       61 aagccaagac aggtgcgaca agatatctga tgagtgttct cgccagagag ctggttgcca
      121 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      181 ggcaaaacgc ccgcaaaaaa ctctcaaggg caaatctgaa gaaaccatct gtggttgatc
      241 ctgaccttat ctggtcatta ccagacggag aaatacgccg ctacgacagg cgtcaaaaca
      301 taatctgtag cgagtgccgg aagagcgaag ctatgcagcg tgtactggct ttctatcagg
      361 gtaattttca gaaggtgctg ttgtgagcca aattaacaat cggaacttcg tgaagagaaa
      421 gcataatcca aaatctgaat aattaagttc agcac
//
LOCUS       DAETQN010000410          444 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.419, whole genome shotgun sequence.
ACCESSION   DAETQN010000410 DAETQN010000000
VERSION     DAETQN010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.419"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>444
                     /locus_tag="K0W68_004798"
     rRNA            <1..>444
                     /locus_tag="K0W68_004798"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          123 a           90 c          139 g           92 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggca
//
LOCUS       DAETQN010000411          440 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.420, whole genome shotgun sequence.
ACCESSION   DAETQN010000411 DAETQN010000000
VERSION     DAETQN010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.420"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..>440)
                     /locus_tag="K0W68_004799"
     CDS             complement(<1..>440)
                     /locus_tag="K0W68_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HBI3076069.1"
                     /translation="MPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWL
                     VRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVL
                     PAPLPPYRVLTGLVDRFGRTQTFHREAAGEFSGEITGVTDGAGR"
BASE COUNT           79 a          154 c          128 g           79 t
ORIGIN      
        1 gacgcccggc accatccgtc acgccggtga tttcgccgct gaattcaccg gcggcttcgc
       61 ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc cggtacggcg
      121 gcagcggcgc aggcagcacc tcatccgctt ccggcacccg ctcacaccag ccgagcagcc
      181 accacggccc ctgcggactg tttgtcgcca gataacgatg cggacttaag cggagttctt
      241 ccggcagcgc ctgccagagt gcggccagcc ggtgaccttc atccagtttc gccacgccgc
      301 cgcgcaccag ccagagtgat tcgctgcggc tgtaaccgtc ctcaccggga aacaggtgct
      361 caaaatacag gcttctgccg ccgttatcac tgagtatcag tgtgttatcg cgcagctgta
      421 agcggatatc cgcaggcatt
//
LOCUS       DAETQN010000412          436 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.421, whole genome shotgun sequence.
ACCESSION   DAETQN010000412 DAETQN010000000
VERSION     DAETQN010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.421"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>436
                     /locus_tag="K0W68_004800"
     CDS             <1..>436
                     /locus_tag="K0W68_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3076070.1"
                     /translation="TLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRH
                     WMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEV"
BASE COUNT          127 a          113 c          122 g           74 t
ORIGIN      
        1 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
       61 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      121 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      181 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      241 atggataaag gttattactc actgggactg ttaaatgcct ggagcctggc gggagaacac
      301 cgccactgga tgatacctct cagaaaggga gcgcaatatg aagagctcag aaaactgggt
      361 aaaggcgatc atctggtgaa gctgaaaacc agcccgcagg cacgaaaaaa gtggccggga
      421 ctgggaaatg aagtga
//
LOCUS       DAETQN010000413          422 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.422, whole genome shotgun sequence.
ACCESSION   DAETQN010000413 DAETQN010000000
VERSION     DAETQN010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.422"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            41..116
                     /locus_tag="K0W68_004801"
     tRNA            41..116
                     /locus_tag="K0W68_004801"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          119 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
      421 aa
//
LOCUS       DAETQN010000414          415 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.423, whole genome shotgun sequence.
ACCESSION   DAETQN010000414 DAETQN010000000
VERSION     DAETQN010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.423"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..230)
                     /locus_tag="K0W68_004802"
     CDS             complement(<1..230)
                     /locus_tag="K0W68_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBI3076071.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSP"
BASE COUNT          109 a           92 c           92 g          122 t
ORIGIN      
        1 aacggactgt ctctcccttg tcgcccatta ggctgatggt gtgttatatc ggtatcaacc
       61 ttatttgggt tgatatagaa agcctccgga accaccctgt aactcctccc gtgtagttca
      121 ggtgcaggat aaatgtttcc attccttgcc catcgtctca gcgttgatat tgatggtggg
      181 ttatccggat atctgagttt tccccacgtt ttgagtgtca caagattcat tgccatacct
      241 ctcacgatat gaccgccagt aaatatacag aatactggcg ggtgtggttg atttttaata
      301 atcagctatg tgaggtagcc tgagtttaac ggacactcct tcctgaaata gaatggcatc
      361 agaaggagct aataatgagc agaaaaaccc aacgttactc taaagagttc aaagc
//
LOCUS       DAETQN010000415          408 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.424, whole genome shotgun sequence.
ACCESSION   DAETQN010000415 DAETQN010000000
VERSION     DAETQN010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.424"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            54..130
                     /locus_tag="K0W68_004803"
     tRNA            54..130
                     /locus_tag="K0W68_004803"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="K0W68_004804"
     tRNA            173..248
                     /locus_tag="K0W68_004804"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAETQN010000416          403 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.425, whole genome shotgun sequence.
ACCESSION   DAETQN010000416 DAETQN010000000
VERSION     DAETQN010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.425"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            12..119
                     /locus_tag="K0W68_004805"
     CDS             12..119
                     /locus_tag="K0W68_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI3076072.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           95 a           92 c          131 g           85 t
ORIGIN      
        1 caggcggcta tatgacgctc gcagaactgg gcatggcctt ctggcatgat ttagcggctc
       61 cggtcattgc tggtattctt gccagtatga tcgtgaactg gctgaacaag cggaagtaac
      121 gtgccatgcg ggcgtcaggc tgccgtaatg gcaatttgcg cccggatcag gccgcagggg
      181 tgaaactctg cggccttttt cgtaagaaaa atgcaacggt tgcaagcgga taatattctg
      241 cggaatgcgc agcacagagt gaagacgtga cggattccgg aacagaaaaa cagtcgtaga
      301 atcagcgtca agccggaagg gttccggtga ggcgcaatgt tgcgggggct ttatccctgg
      361 tggcattggt tgctggagag agaaaacccc cgcacgttgc agg
//
LOCUS       DAETQN010000417          401 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.426, whole genome shotgun sequence.
ACCESSION   DAETQN010000417 DAETQN010000000
VERSION     DAETQN010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.426"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..356)
                     /locus_tag="K0W68_004806"
     CDS             complement(<1..356)
                     /locus_tag="K0W68_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBI3076073.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTA"
BASE COUNT          105 a          101 c          118 g           77 t
ORIGIN      
        1 tgcgctgttt tcgtgaacac gcggcggaca gcctcacttc ccaggcgctg gcgggcctga
       61 ataacggcac tgggggcaac gaaggggcga ttgcccggca gcatgatgtc caggcgattc
      121 acaatctggt gaagaggttc tttacgctca agcgccatgc caacaataca ccagaccatc
      181 atttcgaggg gaagacggcg cttgcgtagc gttacagtac ctgattcggc aaggcaacga
      241 gagatgagtt cggggtcgag gtaatccccc agagaagtca gtgggttacg cagagaatcg
      301 taacgggata ccagatcaag agcctgtcca atgtgcataa aaaaatccgg aaacaagtga
      361 gcgtttccgg attcttacac agccactgga tcggtcaact g
//
LOCUS       DAETQN010000418          399 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.427, whole genome shotgun sequence.
ACCESSION   DAETQN010000418 DAETQN010000000
VERSION     DAETQN010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.427"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>399
                     /locus_tag="K0W68_004807"
     CDS             <1..>399
                     /locus_tag="K0W68_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HBI3076074.1"
                     /translation="QSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSL
                     NDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAGR
                     INSRSTGSVEGGKSRLSVADAITRVMIVGMT"
BASE COUNT          105 a           65 c          110 g          119 t
ORIGIN      
        1 tcaatctaaa agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt
       61 cgattcacta accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc
      121 ttatcgttca ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt
      181 aagtgcatta aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg
      241 tggtatctgg aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc
      301 aggaagaatc aattcacgtt ctactggtag tgttgaaggt ggaaaaagta ggctatctgt
      361 agctgatgca attaccagag taatgattgt cggtatgac
//
LOCUS       DAETQN010000419          398 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.428, whole genome shotgun sequence.
ACCESSION   DAETQN010000419 DAETQN010000000
VERSION     DAETQN010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.428"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..363)
                     /locus_tag="K0W68_004808"
     CDS             complement(<1..363)
                     /locus_tag="K0W68_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="phosphorylates aminoimidazole ribotide (AIR) which
                     forms 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate
                     (HMP-P); synthesis of HMP-P from AIR is complex and
                     requires the product of thiC gene at least; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HBI3076075.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSR"
BASE COUNT          105 a          120 c           89 g           84 t
ORIGIN      
        1 acgcgaaggc tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca
       61 gcagggcaag tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa
      121 tgaacgttcc ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc
      181 ttgcttcaga tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc
      241 aaaaggatca tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc
      301 caccgcaacg ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg
      361 caagagatct acgaccgcat cccctaaaac ccatactt
//
LOCUS       DAETQN010000420          396 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.429, whole genome shotgun sequence.
ACCESSION   DAETQN010000420 DAETQN010000000
VERSION     DAETQN010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.429"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..224
                     /locus_tag="K0W68_004809"
     CDS             <1..224
                     /locus_tag="K0W68_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3076076.1"
                     /translation="RNLTRERTQVGLQAARARGRKGGRPKTLSKDKQALAVQLYNEKK
                     HTVAQICVLMGISRPTLYKYIESARLFKK"
     misc_feature    complement(275..391)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          114 a           94 c          105 g           83 t
ORIGIN      
        1 aacgcaacct gacaagagag agaactcagg tcgggcttca agcagcacga gccagaggac
       61 gtaagggcgg caggccgaaa acactgagta aggataaaca ggcccttgct gttcaactct
      121 ataacgagaa aaaacataca gttgcgcaga tttgtgtgtt gatggggata tcgaggccaa
      181 cgctgtacaa atacatagag tcagccaggt tgttcaagaa atgaacttag cgtacgtttt
      241 cgtacggttg ggcttcaaat ccctaatacg ttgacggggc aatatgcagt tcgctgcata
      301 gcggtccgac cccgtactgc tcacgcagct tatccagcag tggcatcatt ttttccagag
      361 gcggtcgaac tccgccttcg caaaataagc ggaagc
//
LOCUS       DAETQN010000421          395 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.430, whole genome shotgun sequence.
ACCESSION   DAETQN010000421 DAETQN010000000
VERSION     DAETQN010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.430"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
BASE COUNT          126 a          104 c           67 g           98 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcata
//
LOCUS       DAETQN010000422          393 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.431, whole genome shotgun sequence.
ACCESSION   DAETQN010000422 DAETQN010000000
VERSION     DAETQN010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.431"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            63..>393
                     /gene="yhaV"
                     /locus_tag="K0W68_004810"
     CDS             63..>393
                     /gene="yhaV"
                     /locus_tag="K0W68_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HBI3076077.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT          120 a           72 c          100 g          101 t
ORIGIN      
        1 ggaaagaaac ttgtcactgg catggacgtc aacattgatg atgagattgg cgacgacgaa
       61 taatggattt tccacaaagg gttaatggtt gggcgctata tgctcatccc tgttttcagg
      121 aaacctacga cgctttagtt gccgaagtcg agacattaaa gggaaaagat cctgaaaatt
      181 atcagagaaa agccgccaca aagttattgg cggtagtcca taaagtgatt gaggagcata
      241 tcacggtcaa tccatcatca ccggcattcc gtcatggcaa gtcgttaggc tctgggaaaa
      301 ataaagactg gtcacgggta aaatttggtg caggtcgtta tcgtctcttc tttcgttata
      361 gtgaaaaaga gaaagtcatc attctgggat gga
//
LOCUS       DAETQN010000423          390 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.432, whole genome shotgun sequence.
ACCESSION   DAETQN010000423 DAETQN010000000
VERSION     DAETQN010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.432"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..>390)
                     /locus_tag="K0W68_004811"
     CDS             complement(<1..>390)
                     /locus_tag="K0W68_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI3076078.1"
                     /translation="IYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPHFLEHLGNNHL
                     DIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALH
                     GQFDSISMFYLLHCLPGNISTKSCVIR"
BASE COUNT          120 a           78 c           82 g          110 t
ORIGIN      
        1 ttgcgtatta cacagctttt tgtagatata tttccaggca ggcagtgaag aaggtaaaac
       61 atggaaatgg aatcaaattg accatgtaac gccgcgggat aaggttcaaa aacatcatgg
      121 ctaattttat gtttaatttt tgattcccca gcccttgtag atgccgcgtt caggctagct
      181 tcgttcaaat ccattaaaga tatcagacta ctctcaggta cgtgagtaag gtaaaaccca
      241 gttccaacac caatatccag atggttgtta cctaaatgtt ccagaaagtg tggaagaagg
      301 tgttcctttg taggacatcc ccatgcaagc cgatttgata ctcccaaaac ccaccagtca
      361 taaagcttta gggtaagtgg tgtgtaaatt
//
LOCUS       DAETQN010000424          389 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.433, whole genome shotgun sequence.
ACCESSION   DAETQN010000424 DAETQN010000000
VERSION     DAETQN010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.433"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            251..>389
                     /locus_tag="K0W68_004812"
     CDS             251..>389
                     /locus_tag="K0W68_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBI3076079.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RS"
BASE COUNT          121 a           69 c           65 g          134 t
ORIGIN      
        1 ctaaacttaa taaaacctta agattaacat tttaatataa cgagttgcat ttagttacat
       61 gtttaacact tgatttaaga tttgtaatgg ctagattgaa atcagatgta atccattagt
      121 tttatatttt acccatttag ggctgattta ttactacaca cagcagtgca acatctgtca
      181 gtacttctgg tgcttctatt ttagaggcag ctgtcaggtg tgcgatcaat aaaaaaagcg
      241 gggtttcatc atgtttaatg aagtccatag tattcatggt catacattat tgttgatcac
      301 taaaccttct ttgcaggcga cggctctctt gcagcacctt aaacaatcgc tggcaattac
      361 aggaaaatta cataatattc aacgttctc
//
LOCUS       DAETQN010000425          386 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.434, whole genome shotgun sequence.
ACCESSION   DAETQN010000425 DAETQN010000000
VERSION     DAETQN010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.434"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>386
                     /locus_tag="K0W68_004813"
     CDS             <1..>386
                     /locus_tag="K0W68_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3076080.1"
                     /translation="LYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNR
                     FVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIV
                     IPLMIIPMLGSNTVQDLMNTIINMTA"
BASE COUNT           81 a           98 c          102 g          105 t
ORIGIN      
        1 actgtattcc gtctcctcgg tgctgattaa cgtcttcgtc agcagcaaag agttgaaatt
       61 taccggcgga tcggtgcagg tattccacgg catggcggcg tattttggtc taccggaagc
      121 gttgatgaat cgctttgtcg gtctggtgtc ctttaccgcg atgttcggtt ccctgctgat
      181 gtggaccgca acgccggtga aaattttctt ctccgaaatc ccggaaggca tctttggtaa
      241 gaaaaccgtc gaactgaacg aaaacggcgt tccggcgcgc gcagcgtgga tccagttcct
      301 gatcgtcatc ccgctgatga ttatcccgat gctcggttcc aataccgtgc aggatctgat
      361 gaatactatt attaatatga ccgccg
//
LOCUS       DAETQN010000426          374 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.435, whole genome shotgun sequence.
ACCESSION   DAETQN010000426 DAETQN010000000
VERSION     DAETQN010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.435"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..336
                     /gene="istB"
                     /locus_tag="K0W68_004814"
     CDS             <1..336
                     /gene="istB"
                     /locus_tag="K0W68_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HBI3076081.1"
                     /translation="QLSYARVLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSN
                     KGFADWGEMFGDHVLATAILDRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDD
                     EMVESGQHQ"
BASE COUNT          125 a           76 c          101 g           72 t
ORIGIN      
        1 caactgagtt atgcccgggt gttgatcctg gatgaaatag gctatctgcc gatgaacaga
       61 gaggaagcca gtctgttctt ccggctactg aaccgtcgat atgaaaaagc gagcatcata
      121 ctgacgtcaa acaaagggtt cgcagactgg ggagaaatgt tcggagatca cgtactggca
      181 acagcgatcc tggatcggct gctacatcac tcaaccacgc tgaatatcaa aggagagagt
      241 taccggttaa aagagaaacg taaagctgga gtgctgacca aaaacacaac gccaatcagt
      301 gatgatgaaa tggtggaaag cggacagcat cagtaacgaa agtatttagc gggcatgaaa
      361 atggcaaata acgg
//
LOCUS       DAETQN010000427          365 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.436, whole genome shotgun sequence.
ACCESSION   DAETQN010000427 DAETQN010000000
VERSION     DAETQN010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.436"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>365
                     /locus_tag="K0W68_004815"
     CDS             <1..>365
                     /locus_tag="K0W68_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3076082.1"
                     /translation="RYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMATRR
                     GYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWCGD
                     VTYIWTGKRWAYLAVVLDL"
BASE COUNT           87 a           93 c          111 g           74 t
ORIGIN      
        1 agatactgga aaaaccgtcc tgaaaaacca gacggcagac gggctgtatt acgcagtcag
       61 gtacttgagc tacatggcat cagccacggt tcggccggag caagaagcat cgccacaatg
      121 gcaacccgga gaggctacca gatgggacgc tggcttgctg gcaggctcat gaaagagctg
      181 gggctggtca gctgtcagca gccgactcac cggtataaac gtggtggtca tgaacatgtt
      241 gctatcccta actaccttga aaggcagttc gccgtgaccg agccaaatca ggtgtggtgc
      301 ggtgatgtga cctatatctg gacgggtaag cgctgggcgt acctcgccgt tgttctcgac
      361 ctgtt
//
LOCUS       DAETQN010000428          365 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.437, whole genome shotgun sequence.
ACCESSION   DAETQN010000428 DAETQN010000000
VERSION     DAETQN010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.437"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            14..365
                     /locus_tag="K0W68_004816"
                     /pseudo
     CDS             14..365
                     /locus_tag="K0W68_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          124 a           83 c           82 g           76 t
ORIGIN      
        1 cacaggtgct ccaatgaaaa aaagaaattt tagcgcagag tttaaacgcg aatccgctca
       61 actggttgtt gaccagaaat acacggtggc agatgccgcc aaagctatgg atgttggcct
      121 ttccacaatg acaagatggg tcaaacaact gcgtgatgag cgtcagggca aaacaccaaa
      181 agcctctccg ataacaccag aacaaatcga aatacgtaag ctgaggaaaa agctacaacg
      241 cattgaaatg gagaatgaaa tattaaaaaa ggctaccgcg ctcttgatgt cagactccct
      301 gaacagttct cgataatcgg gaaactcaga gcgcattatc ctgtggtcac actctgccat
      361 gtgtt
//
LOCUS       DAETQN010000429          363 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.438, whole genome shotgun sequence.
ACCESSION   DAETQN010000429 DAETQN010000000
VERSION     DAETQN010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.438"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>363
                     /locus_tag="K0W68_004817"
     CDS             <1..>363
                     /locus_tag="K0W68_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3076083.1"
                     /translation="QEKLIWLRAQMKLTSLCWALKELAKDIWSRPWSEERRNDWQRWL
                     SLAANSDVPMMKNVAKTIGKRLYGILNAMRHGVSNGNAEALNSKIRLLRIKAKGYRNR
                     ERFKLGVMFHYGKLNMAF"
BASE COUNT          107 a           76 c          118 g           62 t
ORIGIN      
        1 ggcaggaaaa gctgatatgg ctgcgtgcgc agatgaagct gacgagcctg tgctgggcgc
       61 tgaaagaact ggcaaaggat atctggagca gaccatggag cgaggaaaga cgcaacgact
      121 ggcagagatg gctgtcgctg gcggccaaca gtgacgtccc catgatgaag aatgtggcta
      181 aaacgatagg gaaaaggttg tacggtatcc tgaacgccat gcgccatggc gtatcaaacg
      241 gaaatgcgga agcactgaac agcaagatca ggctgctgag aataaaagcc aagggctacc
      301 gcaaccggga gcgctttaag ctgggagtga tgttccacta tgggaaactg aatatggcgt
      361 tct
//
LOCUS       DAETQN010000430          343 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.439, whole genome shotgun sequence.
ACCESSION   DAETQN010000430 DAETQN010000000
VERSION     DAETQN010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.439"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
BASE COUNT          103 a           79 c           65 g           96 t
ORIGIN      
        1 tcggaaagaa aaaaggccat cagcaaaact ctgatggcct gaatcacctt taccaatatt
       61 gtatgaaaaa acacgcacga ctaattgaca ataattttca tttccattaa aaaagatcgc
      121 gtagcattct taattcatga ggaactttac ccgccagcaa tctgagtagc gaaagctgtt
      181 catccccaat gtttttgctg gcgggtcctt ttttctacgg tcctcctccc cggagggggg
      241 catttcatta tttttctaat cgttactggt gagaaggcgg aagaaaaagc cagttcttcg
      301 cccctaaata acagcatgat tccagtccat gcaaaatctg att
//
LOCUS       DAETQN010000431          338 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.440, whole genome shotgun sequence.
ACCESSION   DAETQN010000431 DAETQN010000000
VERSION     DAETQN010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.440"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..263)
                     /locus_tag="K0W68_004818"
     CDS             complement(<1..263)
                     /locus_tag="K0W68_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBI3076084.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDT"
BASE COUNT           88 a           84 c           66 g          100 t
ORIGIN      
        1 gtgtctggtt cttccggcca ctcaatatca ggtgcagttg atgtatcaac acggttcagc
       61 aacacccgat acttcttcca ggcttccagc aacgagtttt cttcctccgt tgcgatctcc
      121 agatctacag catcctgaag tggcgcaata tgctcactga attcctggat gtagaactgt
      181 gtggtgacgg tcttccagcc attcggctcc tgctgtatcg aagcatacca ggctatttca
      241 atatcgctat gctgcggcag catttaaccc cttgtaattc atcgccataa ttgatttaat
      301 tcacaaataa aactataata tggtgaaatt aatgaaaa
//
LOCUS       DAETQN010000432          336 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.denovo.441, whole genome shotgun sequence.
ACCESSION   DAETQN010000432 DAETQN010000000
VERSION     DAETQN010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.denovo.441"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            43..>336
                     /locus_tag="K0W68_004819"
     CDS             43..>336
                     /locus_tag="K0W68_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBI3076085.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSR"
BASE COUNT           73 a           87 c           96 g           80 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
      301 cgctgggcgt atctgtaccg ggccgtcgac agccgg
//
LOCUS       DAETQN010000433        11014 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.guided.136, whole genome shotgun sequence.
ACCESSION   DAETQN010000433 DAETQN010000000
VERSION     DAETQN010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11014
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.guided.136"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            110..1015
                     /locus_tag="K0W68_004820"
     CDS             110..1015
                     /locus_tag="K0W68_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBI3076086.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1012..2250
                     /locus_tag="K0W68_004821"
     CDS             1012..2250
                     /locus_tag="K0W68_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3076087.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2250..2834
                     /locus_tag="K0W68_004822"
     CDS             2250..2834
                     /locus_tag="K0W68_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBI3076088.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3327..4091)
                     /locus_tag="K0W68_004823"
     CDS             complement(3327..4091)
                     /locus_tag="K0W68_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HBI3076089.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(4209..4274)
                     /locus_tag="K0W68_004824"
                     /pseudo
     CDS             complement(4209..4274)
                     /locus_tag="K0W68_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(4318..4623)
                     /locus_tag="K0W68_004825"
     CDS             complement(4318..4623)
                     /locus_tag="K0W68_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3076090.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            complement(4634..5839)
                     /gene="chrA"
                     /locus_tag="K0W68_004826"
     CDS             complement(4634..5839)
                     /gene="chrA"
                     /locus_tag="K0W68_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HBI3076091.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            complement(5995..6198)
                     /locus_tag="K0W68_004827"
     CDS             complement(5995..6198)
                     /locus_tag="K0W68_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3076092.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            complement(6326..7165)
                     /gene="sul1"
                     /locus_tag="K0W68_004828"
     CDS             complement(6326..7165)
                     /gene="sul1"
                     /locus_tag="K0W68_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HBI3076093.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(7159..7506)
                     /locus_tag="K0W68_004829"
     CDS             complement(7159..7506)
                     /locus_tag="K0W68_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HBI3076094.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            complement(7712..8500)
                     /locus_tag="K0W68_004830"
     CDS             complement(7712..8500)
                     /locus_tag="K0W68_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HBI3076095.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            complement(8631..9104)
                     /locus_tag="K0W68_004831"
     CDS             complement(8631..9104)
                     /locus_tag="K0W68_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HBI3076096.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            9262..10275
                     /gene="intI1"
                     /locus_tag="K0W68_004832"
     CDS             9262..10275
                     /gene="intI1"
                     /locus_tag="K0W68_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBI3076097.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            10478..10828
                     /locus_tag="K0W68_004833"
     CDS             10478..10828
                     /locus_tag="K0W68_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBI3076098.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
BASE COUNT         2289 a         3248 c         3186 g         2291 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttccg gatcggcaac
     1081 gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc atacaaagag cgcggcctgg
     1141 gcgggcgcca cggccgctag cagcgtcatc gcgaccatca tcggcgcgtg ggttggtggt
     1201 ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga tctcgggtgt ggtgggcggc
     1261 gtggccatgg cgagcatccc actgctcgat gccgttggcg ccctctcgaa cactgggctg
     1321 atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg gtatggccgc gcaggacagt
     1381 gagctgccca aactcggcca cgtcgccggg ctctccgttg agcgcgtctc gtcactgaaa
     1441 gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc ttgggggggc cgcaatcggc
     1501 ctgcttggcg ctgcgccaac gctcgggctg acggcgttct gctccgtcct tgcaggtctg
     1561 ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga tgaccacgac ggcgactctc
     1621 tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc tgctgcgccc tctctttggt
     1681 atagtgatga tcttcgtggg catcgttggc gccaacggca gcgtcatcat gcctgcgctg
     1741 tttgtagatg caggacgcca agtagcagag ctcgggctgt tctcctcaat gatgggggct
     1801 ggtggtctcc ttggcattgc cattcatgcg tcggtcggcg cccggatatc agcgcagaac
     1861 tggctggcgg tggcattttg tggctctgcg gtgggctcgc ttctgctttc acagttgcca
     1921 ggcgtgccgg tgctgatgtt gttgggcgcg ctcgtgggac tgctgaccgg ctcagtctct
     1981 cccattctca acgctgccat ctacaaccgc acgccgccag aacttctcgg ccgggtactc
     2041 ggcacggtct cggcggtgat gctgtcagcc tcgcccatgg ttatgcttgc ggccggcgcg
     2101 tttgtcgacc ttgctggtcc gctccctggc ctcgttgtat cggccgtgtt tgcggggctc
     2161 gtggctctac tctcgctccg tcttcaattt gctacaatgg cggcggcagc cacagcctcc
     2221 gccccaaccc atacagaagg tgaacactga tgccccgccc caagctcaag tccgatgacg
     2281 aggtactcga ggccgccacc gtagtgctga agcgttgcgg tcccatagag ttcacgctca
     2341 gcggagtagc aaaggaggtg gggctctccc gcgcagcgtt aatccagcgc ttcaccaacc
     2401 gcgatacgct gctggtgagg atgatggagc gcggcgtcga gcaggtgcgg cattacctga
     2461 atgcgatacc gataggcgca gggccgcaag ggctctggga atttttgcag gtgctcgttc
     2521 ggagcatgaa cactcgcaac gacttctcgg tgaactatct catctcctgg tacgagctcc
     2581 aggtgccgga gctacgcacg cttgcgatcc agcggaaccg cgcggtggtg gaggggatcc
     2641 gcaagcgact gcccccaggt gctcctgcgg cagctgagtt gctcctgcac tcggtcatcg
     2701 ctggcgcgac gatgcagtgg gccgtcgatc cggatggtga gctagctgat catgtgctgg
     2761 ctcagatcgc tgccatcctg tgtttaatgt ttcccgaaca cgacgatttc caactcctcc
     2821 aggcacatgc gtaaacggag gtgtgcagag tccctgcggc aggcgacgaa cacgaccgtc
     2881 gtcgattagt accggtacgg tcggtggtat cgaagtcttg atcaccactc aggtctacgg
     2941 cttacaaatg gtgaccatcc cgatacttgc gtcagagcac cgggccgatt ctttgacagt
     3001 gaatcactcc cgtaaggttg tgccggtgtg ggtgtcccgg gtcgagacga tactccgcca
     3061 atgcgcccag caaacaacct ggccatcgca ggtggtgggg agcggtgtgg cggatgagtt
     3121 ggacaagttg gtgtagcagc acgagcacgg cgagataaca tcgcaggagt tcgacatgct
     3181 caagagacag ctgattgcga atcgcgatgc agattcataa cccgattgcg ggttggcttc
     3241 actccaccat caccgagcag actagcacgg cgggctctgt tgcaaagatt ggcggcagtc
     3301 agaggtaggc tgtcgctctg cgccgatcag gcggctgctg cgaaatggtg gttgagcatg
     3361 cccatggcct ccgtcagcgc cgagggccca atgccaaaag ctctctccac aaggcgcacc
     3421 tcgcccctga tgccgggctg caggcaccag gggcgagcct gtcctttgcg cagggctcgc
     3481 atgacttcga atcccttgat cgtggcatag gccgtgggga tcgatttgaa accgcgcacc
     3541 ggcttgatca gtatcttgag ctttccgtga tcggcctcga tcacgttatt gagatacttc
     3601 acctgccggt gggccgtctc ccggtccagc tttccttcgc gcttcaattc ggtgatcgct
     3661 gcaccatagc tcggcgcttt gtcggtattg agcgtggcag gcttttccca gtgcttcagg
     3721 cctcgcaggg ccttgcccag gaaccgcttc gctgccttgg cgctgcgggt cggcgacagg
     3781 tagaaatcga tcgtgtcgcc ccgcttgtcg actgcccggt acaggtaggt ccacttgccc
     3841 cgcaccttga cgtaggtttc atccaggcgc cagctcggat caaagccacg ccgccagaac
     3901 cagcgcagcc gcttctccat ctccggggcg tagcactgga cccagcgata gatcgtcgta
     3961 tggtcgaccg aaatgccgcg ttccgccagc atttcctcaa ggtcgcgata gctgatcgga
     4021 tagcgacaat accagcgcac cgcccacagg atcacatcac cctggaaatg gcgccacttg
     4081 aaatccgtca tcgttccgtc cgtccaatct ccgccaagca tgctcaagct tcacgatttt
     4141 tgcaacagag cccacacgag tattgagcat agtcgagatt ggtgcagatc acttctgata
     4201 ttgaactgtc aggagctggc tgcacaacag ccattacgcc caatcaactg gtgcagtcgt
     4261 cttctgaaaa tgacaatcca gttagggtat agctcaacct gacatagaag caaaaactca
     4321 accaccttct accaactctc cgaacagctc cttgaccttt gttttcgcat cagcaagtgc
     4381 agttctgcct tgttcagtga tgtcataaac acgccgttca cgtcgcccgg tgcgttcgtg
     4441 gcgtgaggtc agatagcctt ttttttccag gccgtgcagc atcgggtaca cggtgccagc
     4501 gctcatctcg tagccgtgtc ggcgtagctc ttcgatgatc cccagcccaa agacaggttc
     4561 ctcggctgca tggtgaagga tgtgcaggcg gatcaaaccg ccgtagaggt ctttgtcagt
     4621 cattttttgt gcctcacaga gcgacgctca acagccaccc agctgcaccg ctaccgagga
     4681 caaccagcca cggcgggagc ttccagaaca taagtgcgac aagggcaact aatgccaagc
     4741 cgaagtcttg cggctgaaag atggcgctag tccatacagg ctgatacagc gcggccagca
     4801 gcaagccgac tacagcggca ttgatcccgg ccagcgcagc ttggatgcct gtattgcggc
     4861 gcaaacgctc ccaaaatggc attgatccga cgaccagcaa gaacgagggc gcgaagatag
     4921 ccagcagaca cacaatgccg ccgatccagc ccgacggggc ggtgttcatc gaggcaccaa
     4981 gaaacgcggc gaacgtgaac aaagggccgg gcaccgcttg agctgccccg taccccgcga
     5041 gaaaggattc attgttgacc cagccggagg gcaccacttc ggcttgcagt aatggcagca
     5101 caacgtgacc accgccgaac accagtgatc cgacacgata gaaggaatcc accattgcca
     5161 tggtttgact tggcatcagt tcggccaaca ccggcaggcc aatcagcaag acaaagaaca
     5221 gcgagagcca aagcacgccg gcccggtgac tgaccgtgat aggtaggggg tcatgctcaa
     5281 caactttcgc tggcttgaac aataaccggc ctgcgatgcc tgcgatagca atcacgccaa
     5341 cctgtcccca cgcggacggc acaagtaaaa cgacgcaggt agcaattgcc atgatggtga
     5401 ctcgcagccc atccgtgcat aggttacgcg ccatgcccca tactgcttga gcgaccacgg
     5461 ccacagccac cacttttaag ccatgcaacg cgccctgcga gacgtaatcg ccatagctgg
     5521 agatgccgag cgcaaaaagg atcaaggcta tggcagacgg cagcgtgaag ccagcccaag
     5581 cagccagcgc cccgctgtat ccagcccgag acagtcctac cgctatgccg acctggctgc
     5641 ttgcaggccc tggcaagaac tgacaaagcg cgaccaagtc agcatagctc cgttcggaga
     5701 gccagcgccg ccgtgtgaca aattcggcgc ggaagtagcc caagtgcgca atggggccgc
     5761 caaaagatgt caatccaagc cgcagaaaaa taagaaagac cgaccatggt ctgctgtcat
     5821 cggtagggtt attcgtcata ctttcgcctt catgatctgc aacgagttga tcaataataa
     5881 gcgaaattcg ataacgaaat tcgatataaa tctagaaaaa aatacctcta tgtgtactac
     5941 gcagttttag ctgtggcttt cacaggagca cgcttactta cggcttagcg tgctttattt
     6001 tccgttttct gaggcgatcc ctaggagctc ggatctcagg acgaaggtct ccgcgaatgt
     6061 ccggtcgatc cgcgcgacgt cccaggcggg cgttcccttg gcggacatcc acgccgcagc
     6121 gtcgtgcatc agccgcacaa cctcgtcgat atcacccgag caggcgaccc gaacgttcgg
     6181 aggctcctcg ctgtccattc gctcccctgg cgcggtatga accgccgcct catagtgcag
     6241 tttgatcctg acgagcccag catgtctgcg cccaccttcg cggaacctga ccagggtccg
     6301 ctagcgggcg gccggaaggt gaatgctagg catgatctaa ccctcggtct ctggcgtcgc
     6361 gactgcgaaa tttcgcgagg gtttccgaga aggtgattgc gcttcgcaga tctccaggcg
     6421 cgtgggtgcg gacgtagtca gcgccattgc cgatcgcgtg aagttccgcc gcaaggctcg
     6481 ctggacccag atcctttaca ggaaggccaa cggtggcgcc caagaaggat ttccgcgaca
     6541 ccgagaccaa tagcggaagc cccaacgccg acttcagctt ttgaaggttc gacagcacgt
     6601 gcagcgatgt ttccggtgcg gggctcaaga aaaatcccat ccccggatcg aggatgagcc
     6661 ggtcggcagc gaccccgctc cgtcgcaagg cggaaacccg cgcctcgaag aaccgcacaa
     6721 tctcgtcgag cgcgtcttcg ggtcgaaggt gaccggtgcg ggtggcgatg ccatcccgct
     6781 gcgctgagtg cataaccacc agcctgcagt ccgcctcagc aatatcggga tagagcgcag
     6841 ggtcaggaaa tccttggata tcgttcaggt agcccacgcc gcgcttgagc gcatagcgct
     6901 gggtttccgg ttggaagctg tcgattgaaa cacggtgcat ctgatcggac agggcgtcta
     6961 agagcggcgc aatacgtctg atctcatcgg ccggcgatac aggcctcgcg tccggatggc
     7021 tggcggccgg tccgacatcc acgacgtctg atccgactcg cagcatttcg atcgccgcgg
     7081 tgacagcgcc ggcggggtct agccgccggc tctcatcgaa gaaggagtcc tcggtgagat
     7141 tcagaatgcc gaacaccgtc accatggcgt cggcctccgc agcgacttcc acgatgggga
     7201 tcgggcgagc aaaaaggcag caattatgag ccccatacct acaaagcccc acgcatcaag
     7261 cttttgccca tgaagcaacc aggcaatggc tgtaattatg acgacgccga gtcccgacca
     7321 gactgcataa gcaacaccga cagggatgga tttcagaacc agagaaagaa aataaaatgc
     7381 gatgccataa ccgattatga caacggcgga aggggcaagc ttagtaaagc cctcgctaga
     7441 ttttaatgcg gatgttgcga ttacttcgcc aactattgcg ataacaagaa aaagccagcc
     7501 tttcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa
     7561 gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgcata
     7621 gttgagcggc gggcgcagcc cgtccgcttg aacgccgagt taggcatcag atgccctcgg
     7681 cgcgggtcga tgcacttttc gcacatgccg ctcaacgcaa gattctctca atcgttgctt
     7741 tggcatatcg aacgaacgcg gccgtctctt cgacgcgcat tgctaggtcg tcgtcctcgc
     7801 tacccaggta cgccgcgcgt gccttgcaga tgaggggccg atgctcggca ggcaaacgct
     7861 ccgataccca tgcggcagca acgtccttag gagcaatgag accagttgaa gcgctgtacc
     7921 aaatgcgagc aagagcaaga acgacgttcc gctcgtcacc cttccaatcc gactctgcat
     7981 tccactgggc aatagtgtcg aaaagcgcct tggagaaatg ctccttcggc accggctcga
     8041 aaaacgtggc tgcggatggg cctagaagcg caaggctgtg ttgcctcgcc ttggtcagca
     8101 aaatcgcaag atcgtgatcc agaacggcag gctcgaacgt tccggaaagg atgtcgtggc
     8161 ggagccactc accgaactga agctcacgcc gcgccggata gcgccaaggc actacttcgc
     8221 ttcgagcgac aacagttagc tccagcggtc gccatgttcc gccatcgcct ggcggtgatg
     8281 agactttcag caaatcgagc attagcgcct gccggagcga atcgttaggt gcggcgctga
     8341 cggtcacgag caagtctatg tcgctgtccg gcttcagccc tccatcgatc gcagatccga
     8401 acaggtggat tgtgtccagt gtcgcagcca gatggcgctc gatcaccgcg cgagcgtggg
     8461 acagctgctt gaaaacttgt gcagggaaaa attcacccat gatgcctaac gttaagttca
     8521 gcggcagctt ttaagttgcg gctttgtgga atacttttgc gcagcaaaac cacaaagacg
     8581 cgacttaaaa gctgtccaag gagcgaagcg actggtgctg caacgcattg ttagcctttt
     8641 ttccaaatct ggtatgtata atttatatta gacataaaaa actgttcaaa aaccaaattg
     8701 aaattctcag gcattatagg gaatttgata tcaccttcga cttcaacgtg aacagtagac
     8761 aaatgaatta tatctgcttt ttcaataagg ctattataga tttgaccccc gccagagaca
     8821 tatacatgat ctgtaacttt tgatagctct ttcaaagcat tttctattga aggaaaaact
     8881 aggacgtttt catttgagct tgaaattccg ttctttgaca ctactgcata tttgcgattt
     8941 ggaagaacac ccatagagtc aaatgttttt cttccgacaa ggagccattg attatatgtg
     9001 agcgctttaa agagtagttg ctcacctttt actgaccacg ggatatcagg accactaccg
     9061 attacgccat tttctgacac tgcagaaatc aatgatattt tcaatttaac tcccttaatg
     9121 gctaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc
     9181 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca
     9241 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     9301 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc
     9361 gaacaggctt atgtccactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg
     9421 gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag
     9481 gtttcggtct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg
     9541 ctgtgcacgg atctgccctg gcttcaggag atcggaagac ctcggccgtc gcggcgcttg
     9601 ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga aggcgagcat
     9661 cgtttgttcg cccagcttct gtatggaacg ggcatgcgga tcagtgaggg tttgcaactg
     9721 cgggtcaagg atctggattt cgatcacggc acgatcatcg tgcgggaggg caagggctcc
     9781 aaggatcggg ccttgatgtt acccgagagc ttggcaccca gcctgcgcga gcagctgtcg
     9841 cgtgcacggg catggtggct gaaggaccag gccgagggcc gcagcggcgt tgcgcttccc
     9901 gacgcccttg agcggaagta tccgcgcgcc gggcattcct ggccgtggtt ctgggttttt
     9961 gcgcagcaca cgcattcgac cgatccacgg agcggtgtcg tgcgtcgcca tcacatgtat
    10021 gaccagacct ttcagcgcgc cttcaaacgt gccgtagaac aagcaggcat cacgaagccc
    10081 gccacaccgc acaccctccg ccactcgttc gcgacggcct tgctccgcag cggttacgac
    10141 attcgaaccg tgcaggatct gctcggccat tccgacgtct ctacgacgat gatttacacg
    10201 catgtgctga aagttggcgg tgccggagtg cgctcaccgc ttgatgcgct gccgcccctc
    10261 actagtgaga ggtagggcag cgcaagtcaa tcctggcgga ttcactaccc ctgcgcgaag
    10321 gccatcggtg ccgcatcgaa cggccggttg cggaaagtcc tccctgcgtc cgctgatggc
    10381 cggcagcagc ccgtcgttgc ctgatggatc caacccctcc gctgctatag tgcagtcggc
    10441 ttctgacgtt cagtgcagcc gtcttctgaa aacgacaatg gaggtggtag ccgagggtgt
    10501 ggaaacaccc gactgccttg cgtggttgcg gcaggcgggt tgcgacacgg tgcagggttt
    10561 cctgttcgcc aggccgatgc cggcggcggc cttcgtcggc ttcgtcaacc aatggaggaa
    10621 caccaccatg aacgccaatg aaccgagcac cagttgctgc gtgtgctgca aggaaatccc
    10681 gctcgatgcc gccttcacgc cggaaggggc cgagtacgtg gagcatttct gcgggctgga
    10741 gtgctatcag cgcttccagg cgcgggccag cactgcgacc gaaaccagcg tcaaaccgga
    10801 cgcttgtgat tcgccgccgt caggttgagg cataccctaa cctgatgtca gatgccatgt
    10861 gtaaattgcg tcaggatagg attgaatttt gaatttattg acatatctcg ttgaaggtca
    10921 tagagtcttc cctgacattt tgcagggaat tccatgactg gacagcgcat tgggggcact
    10981 gttgcaaagt tagcgatgag gcagcctttt gtct
//
LOCUS       DAETQN010000434         9555 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.guided.154, whole genome shotgun sequence.
ACCESSION   DAETQN010000434 DAETQN010000000
VERSION     DAETQN010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.guided.154"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(46..1023)
                     /gene="epmA"
                     /locus_tag="K0W68_004834"
     CDS             complement(46..1023)
                     /gene="epmA"
                     /locus_tag="K0W68_004834"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBI3076099.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1348..3156
                     /gene="frdA"
                     /locus_tag="K0W68_004835"
     CDS             1348..3156
                     /gene="frdA"
                     /locus_tag="K0W68_004835"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBI3076100.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3149..3883
                     /gene="frdB"
                     /locus_tag="K0W68_004836"
     CDS             3149..3883
                     /gene="frdB"
                     /locus_tag="K0W68_004836"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBI3076101.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            3894..4289
                     /gene="frdC"
                     /locus_tag="K0W68_004837"
     CDS             3894..4289
                     /gene="frdC"
                     /locus_tag="K0W68_004837"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBI3076102.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4300..4659
                     /gene="frdD"
                     /locus_tag="K0W68_004838"
     CDS             4300..4659
                     /gene="frdD"
                     /locus_tag="K0W68_004838"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBI3076103.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            4722..5855
                     /locus_tag="K0W68_004839"
     CDS             4722..5855
                     /locus_tag="K0W68_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBI3076104.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            5944..6477
                     /gene="blc"
                     /locus_tag="K0W68_004840"
     CDS             5944..6477
                     /gene="blc"
                     /locus_tag="K0W68_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBI3076105.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6474..6791)
                     /gene="sugE"
                     /locus_tag="K0W68_004841"
     CDS             complement(6474..6791)
                     /gene="sugE"
                     /locus_tag="K0W68_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBI3076106.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(6966..7112)
                     /gene="ecnB"
                     /locus_tag="K0W68_004842"
     CDS             complement(6966..7112)
                     /gene="ecnB"
                     /locus_tag="K0W68_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBI3076107.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7223..7348)
                     /gene="ecnA"
                     /locus_tag="K0W68_004843"
     CDS             complement(7223..7348)
                     /gene="ecnA"
                     /locus_tag="K0W68_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBI3076108.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7400..7966)
                     /gene="efp"
                     /locus_tag="K0W68_004844"
     CDS             complement(7400..7966)
                     /gene="efp"
                     /locus_tag="K0W68_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBI3076109.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8008..9036
                     /gene="epmB"
                     /locus_tag="K0W68_004845"
     CDS             8008..9036
                     /gene="epmB"
                     /locus_tag="K0W68_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBI3076110.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9426..9521
                     /locus_tag="K0W68_004846"
                     /pseudo
     CDS             9426..9521
                     /locus_tag="K0W68_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2403 a         2443 c         2444 g         2265 t
ORIGIN      
        1 gcttatcacg ccattcttcg ctgttaattc agtaattttt cagaattatg cccggtcaac
       61 gctaaaggcg atgacttcag ccagtgtctc cgcgcccagc gccaacatca ccagacgatc
      121 aacacctaat gccacgccgg aacagtcagg cataccgact ttcaaggctt caatcagatt
      181 ctggtcaatg gggtgctgcg gcaaaccgcg cgccgcgcgc ttacggttat cttgttcaaa
      241 gcgttgttgc tgctcacggg catccgttaa ttcatggaaa ccattcgcca gctcaatacc
      301 tttataataa acctcaaagc gttcagcgac ccgatgatct tcggtactga tttgcgccag
      361 tgatgcctgg ctggctggaa agtggtacac aaaggtcggt ttttctttgc caatatttgg
      421 ctctacgcca aaggtaaaca gcaattgtag caacgtgtcg cggtcttctt cggtatcagc
      481 aacgttactc aaatccagtt tcgctgcgac ttcccgcagt tgcgttttgt cggcagagag
      541 cgggtcaatt tccagataac gcaagaaagc ttgttgataa gaaaggcttt ctgctgccgg
      601 gcagtccagc acctgttgta agagatcgtc cacctcgttc atcaaccggt acatatcata
      661 gtgcggtcga taccactcaa gcatagtgaa ctcagggttg tgataacgcc ccatctcttc
      721 attacggaag ctgcggcaca gctggaatac cggcccacaa ccggcaacca gcaggcgttt
      781 catatggtat tccgggctgg tcattaacca gagattcatc ccctgcgaat gcccggggcc
      841 aacgaaacgt gtctcaaacg ggaccaaatg aatatcggtt accgtcgcct ggctcataca
      901 aggcgtctcc acctccagca ctccacgatc ggcaaagaaa cgacggatct ccgccataat
      961 cgccgcacgt tttaataagt taggaatgga tgcgctcggc tgccaggatg ccgtttcgct
     1021 catagttaaa tctccagttt ttgacaaggg cacgaagtct actcgcaacg cgacggcgag
     1081 acaaatttta cgcaggaatc aaacagcggt gggcagtgac taaaaaaagc acgatctgat
     1141 ggtttagtaa ttaaattaat catcttcagt gataatttag ccctcttgcg cactaaaaaa
     1201 atcgatctcg tcaaatttca gacttatcca tcagactata ctgttgtacc tataaaggag
     1261 cagtggaata gcgttcgcag accgtaactt tcaggcactt accctgaagt acggggctgt
     1321 gggataaaaa caatctggag gaatgtcgtg caaacctttc aagccgatct tgccattgta
     1381 ggcgccggtg gcgcgggatt acgtgctgca attgctgccg cgcaggcaaa tccaaatgca
     1441 aaaatcgcac taatctcaaa agtatacccg atgcgtagcc ataccgttgc tgcagaaggg
     1501 ggctccgccg ctgtcgcgca ggatcatgac agcttcgaat atcactttca cgatacagta
     1561 gcgggtggcg actggttgtg tgagcaggat gtcgtggatt atttcgtcca ccactgccca
     1621 accgaaatga cccaactgga actgtggggg tgcccatgga gccgtcgccc ggatggtagc
     1681 gtcaacgtac gtcgcttcgg cggcatgaaa atcgagcgca cctggttcgc cgccgataag
     1741 accggcttcc atatgctgca cacgctgttc cagacctctc tgcaattccc gcagatccag
     1801 cgttttgacg aacatttcgt gctggatatt ctggttgatg atggtcatgt tcgcggcctg
     1861 gtagcaatga acatgatgga aggcacgctg gtgcagatcc gtgctaacgc ggtcgttatg
     1921 gctactggcg gtgcgggtcg cgtttatcgt tacaacacca acggcggcat cgttaccggt
     1981 gacggtatgg gtatggcgct aagccacggc gttccgctgc gtgacatgga attcgttcag
     2041 tatcacccaa ccggtctgcc aggttccggt atcctgatga ccgaaggctg ccgtggtgaa
     2101 ggcggtattc tggtcaacaa aaatggctac cgttatctgc aagattacgg catgggcccg
     2161 gaaactccgc tgggcgagcc gaaaaacaaa tatatggaac tgggtccacg cgacaaagtt
     2221 tctcaggcct tctggcacga atggcgtaaa ggcaacacca tctccacgcc gcgtggcgat
     2281 gtggtttatc tcgacttgcg tcacctcggc gagaaaaaac tgcatgaacg tctgccgttc
     2341 atctgcgaac tggcgaaagc gtacgttggc gtcgatccgg ttaaagaacc gattccggta
     2401 cgtccgaccg cacactacac catgggcggt atcgaaaccg atcagaactg tgaaacccgc
     2461 attaaaggtc tgttcgccgt gggtgaatgt tcctctgttg gtctgcacgg tgcaaaccgt
     2521 ctgggctcca actccctggc ggaactggtg gtcttcggcc gtctggccgg tgaacaagcg
     2581 acagagcgtg cagcaactgc cggtaatggc aacgaagcgg caattgaagc gcaggcagct
     2641 ggcgttgaac aacgtctgaa agatctggtt aaccaggatg gcggcgaaaa ctgggcgaag
     2701 atccgcgacg aaatgggcct ggcaatggaa gaaggttgcg gtatctaccg tacgccggaa
     2761 ctgatgcaga aaaccatcga caagctggca gagctgcagg aacgcttcaa gcgcgtgcgc
     2821 atcaccgaca cctccagcgt gttcaacacc gacctgctct acaccattga actgggccac
     2881 ggtctgaacg ttgctgaatg tatggcgcac tccgcaatgg cacgtaaaga gtcccgcggc
     2941 gcacaccagc gtctggacga aggttgcacc gagcgtgacg acgtcaactt cctcaaacac
     3001 accctcgcct tccgcgatgc tgatggcacg actcgcctag agtacagcga cgtgaagatt
     3061 actacgctgc cgccagctaa acgcgtttac ggtggcgaag cggatgcagc cgataaggcg
     3121 gaagcagcca ataagaagga gaaggcgaat ggctgagatg aaaaacctga aaattgaggt
     3181 ggtgcgctat aacccggaag tcgataccgc accgcatagc gcattctatg aagtgcctta
     3241 tgacgcaact acctcattac tggatgcgct ggtctacatc aaagacaacc tggcaccgga
     3301 cctgagttac cgctggtcct gccgtatggc gatttgtggt tcctgcggca tgatggttaa
     3361 caacgtgcca aaactggcat gtaaaacctt cctgcgtgat tacaccgacg gtatgaaggt
     3421 tgaagcgtta gctaacttcc cgattgaacg cgatctggtg gtcgatatga cccacttcat
     3481 cgaaagtctg gaagcgatca aaccgtacat catcggcaac tcccgcaccg cggatcaggg
     3541 tactaacatc cagaccccgg cgcagatggc aaagtatcac cagttctccg gttgcatcaa
     3601 ctgtggtctg tgctacgccg cgtgcccgca gtttggcctg aacccagagt tcatcggtcc
     3661 ggctgccatt acgctggcgc atcgttataa cgaagatagc cgcgaccacg gtaagaagga
     3721 gcgtatggcg cagttgaaca gccagaacgg cgtatggagc tgtactttcg tgggctattg
     3781 ctccgaagtc tgcccgaaac acgtcgatcc ggctgcggcc attcagcagg gcaaagtaga
     3841 aagttcgaaa gactttctta tcgcgaccct gaaaccacgc taaggagtgc aacatgacga
     3901 ctaaacgtaa accgtatgta cggccaatga cgtccacctg gtggaaaaaa ttgccgtttt
     3961 atcgctttta catgctgcgc gaaggcacgg cggttccggc tgtgtggttc agcattgaac
     4021 tgattttcgg gctgtttgcc ctgaaaaatg gcccggaagc ctgggcggga ttcgtcgact
     4081 ttttacaaaa cccggttatc gtgatcatta acctgatcac tctggcggca gctctgctgc
     4141 acaccaaaac ctggtttgaa ctggcaccga aagcggccaa tatcattgta aaagacgaaa
     4201 aaatgggacc agagccaatt atcaaaagtc tctgggcggt aactgtggtt gccaccatcg
     4261 taatcctgtt tgttgccctg tactggtaag gagcctgaga tgattaatcc aaatccaaag
     4321 cgttctgacg aaccggtatt ctggggcctc ttcggggccg gtggtatgtg gagcgcaatt
     4381 attgcgccgg tgatgatcct gctggtgggt attctgctgc cactggggct gtttccgggt
     4441 gatgcgctga gctacgagcg cgttctggcg ttcgcgcaga gcttcattgg tcgcgtattc
     4501 ctgttcctga tgatcgttct gccgctgtgg tgtggtttac accgtatgca ccacgcgatg
     4561 cacgatctga aaattcacgt gcctgcgggc aaatgggttt tctacggtct ggctgctatc
     4621 ctgacagttg tcacgctgat tggtatcgtt acaatctaac gtatcgccaa tgtaaatccg
     4681 gcccgcctat ggcgggccgt tttgtatgga aaccagacct tatgttcaaa acgacgctct
     4741 gcgccttatt aattaccgcc tcttgctcca catttgctgc ccctcaacaa atcaacgata
     4801 ttgtgcatcg cacaattacc ccgcttatag agcaacaaaa gatccccggt atggcggtgg
     4861 cggtaattta tcagggtaaa ccttattact ttacctgggg ctatgcggac atcgccaaaa
     4921 agcagcccgt cacacagcaa acgttgtttg agttaggttc ggtcagcaaa acatttacgg
     4981 gcgtgcttgg tggcgacgct attgctcgag gggaaatcaa gttaagcgat cccgcaacaa
     5041 aatactggcc tgaacttact gctaaacagt ggaatgggat cacactatta catcttgcga
     5101 cctacaccgc tggcggcctg ccattgcagg tgccggatga agtgaaatcc tcaagcgact
     5161 tgctgcgctt ctatcaaaac tggcagcctg catgggctcc aggaacacaa cgtctgtatg
     5221 ccaactccag tatcggtttg ttcggcgcac tggctgtgaa gccgtctggt ttgagttttg
     5281 agcaggcgat gcaaactcgt gtcttccagc cactcaaact caaccatacg tggattaatg
     5341 tacctccccc agaagaaaag aattacgcct ggggatatcg cgaaggtaag gcagtgcatg
     5401 tttcgccagg ggcgttagat gctgaagctt atggtgtgaa gtcgaccatt gaagatatgg
     5461 cccgctgggt gcaaagcaat ttaacccccc ttgatatcaa tgagaaaacg cttcaacaag
     5521 ggatacaact ggcacaatct cgctactggc aaaccggcga tatgtatcag ggcctgggct
     5581 gggaaatgct ggactggccg gtcaatcctg acagcatcat taacggcagt gacaataaaa
     5641 ttgcactggc agcacgcccg gtaaaagcga ttaagccccc aactcctgca gtacgcgcat
     5701 catgggtaca taaaacgggg gcgaccggcg gatttggtag ctatgtcgcg tttattccag
     5761 aaaaagagct gggtatcgtg atgctggcta acaaaaacta tcccaatcca gcgagagtcg
     5821 ccgccgcctg tcagattctc aacgctctac agtaaaattt catcgggtcc gaattttcgg
     5881 accttttctg cgcttttcct tgctgtcatc tacacttaga aaaaaaccag taaggaaaca
     5941 tttatgcgcc tgctccctct cgttgccgca gcgacagctg catttctggt cgttgcctgc
     6001 agttctccta cgccgccgcg tggcgtgaca gtagtgaata atttcgacgc taaacgctat
     6061 ctcggtacct ggtatgagat tgcccgtttt gatcaccgtt ttgaacgtgg actggaaaaa
     6121 gtcaccgcaa catacagtct gcgtgatgac ggcggcctga atgtcatcaa taaaggctat
     6181 aaccctgaca gagaaatgtg gcagcagagt gaagggaaag cgtactttac cggcgcacca
     6241 acacgcgctg cgctgaaagt gtcattcttt ggtcctttct atggcggtta taacgtcatt
     6301 gcacttgatc gggaataccg ccatgcgctg gtttgcggcc cggaccgcga ctacctgtgg
     6361 atactctccc gcacgccaac catttctgac gaagtgaaac aggagatgct ggcagtcgcg
     6421 acccgggaag ggtttgatgt cagtaagttt atttgggtac agcagcccgg tagttagtga
     6481 gtgctgagtt tcagaccaat aatccccaat acgattaacg ccagactcgc caggcgcatc
     6541 gggttagcgg actcaccgag cagcacaatg ccggtgatgg ccgcgccgac tgcgccaata
     6601 cccgtccaca cggcataagc cgtccctacc ggtaacgatt tcatcgccca ggcaagtagc
     6661 gccatactga ctatcatcgc cgtcacagta ataacacttg gcgtcaaacg actaaagccg
     6721 tgggtatatt tcaggccaac ggcccatacc acttccagca gaccagcaat aactaagata
     6781 atccaggaca tatcaggctc cggagaattg gggccatccc cggtgaaaag aggcgtttgc
     6841 aggtcgtcct gcaaagctga tggttgaacg gatattttgc caggtggcag gggaatttca
     6901 actattacct ttgtgactat cgcaaaaaaa caaacggcac gacataactg tcgtaccgtt
     6961 attgcttatt gctgcgcttt cgttgctgca ccagaaatcg cgttaccgcc atcagaaatg
     7021 tcttcaccaa cgccacgcgt ggtgttgcag gcagttaata ctgttgaaag caccagaaca
     7081 gaaaagatcg ctgcaattgt cttcttcacc ataacgtctt ccttttaggt aatgttattt
     7141 atgtttgcct atagcaaatt aataatagac aacatcgcca aaaatgatgg aaatctgatg
     7201 atttttcgga agagaagaaa aattagctgg cagcgcggga gatggagttg ccgagatgtt
     7261 tgatgtcttc gccgaaaccg cgagcggtgt tacagcccgt gagcagcgtg ctggcaagca
     7321 aaacaagaac gataaggcgt ttcatcatct ctgacctgaa taagtgatgg tgcagcctgc
     7381 aggccgcacc acaaccgcat tacttcacgc gagagacgta ttcaccagag cgggtatcca
     7441 ctttgatgac ttcgccgatt tgtacgaaca gcggaacttt aaccacagcg ccagtagaca
     7501 gggtagccgg tttgccgcca gtacctgcgg tatcaccttt caggcccgga tcggtatcaa
     7561 cgatttccag ttcaacgaag ttcggcggag taacggagat cggctgacca ttccacagag
     7621 ttacgataca ctctgcctga tccagcagcc atttagcgtt gtcgccaatt gcttttgcat
     7681 cagcagacag ctgctcgaaa gtttcgttgt tcatgaagtg ccagaactca ccgtcgttgt
     7741 acaggtaagt caggttcata tcgacaacat cagcgccttc agcggaatca gtagatttga
     7801 aggttttttc tacgcgagta ccggtcagca gacgacgcag tttaacgcga gcaaatgcct
     7861 ggcctttacc cggttttacg aattcactcg cttcaaccgc gtaaggttcg ccgtctaaca
     7921 tgattttaag accagcacga aaatcgttgc tatagtacgt tgccataagg ccctctgaaa
     7981 tttgttaatt ggtagctaag ccacaaaatg gcgcatattg taaccctaaa taccccatcc
     8041 agagaagatt ggttaacgca acttgccgat gttgtgaccg atcctggtga acttctgcgt
     8101 cttttgaata tagacgcgga cgaaaaactg ttggccggac gcagcgccaa aaagctgttt
     8161 gccctgcgtg tgccccgctc atttatcgat cgcatggaga aaggcaatcc ggacgatcct
     8221 attttgcgtc aggtacttac ctcgcaagat gagtttgtcg tcgcgtccgg attctccacc
     8281 gacccactgg aagaacagca cagcgtagtg cctggtttgt tgcataaata ccacaaccgg
     8341 gcgcttttgc tggtcaaagg cggctgcgcg gtaaattgcc gctattgctt ccgtcgccac
     8401 ttcccctatg ccgaaaatca gggcaacaag cgtaactggc agactgcact tgagtatgtt
     8461 gctgcgcatc cggaactgga cgagatgatt ttctccggcg gcgatccgct gatggcgaaa
     8521 gatcacgagc tggactggtt gctcacacaa ctggaagcca tcccgcatat caaacgtctg
     8581 cggattcaca gccgtctgcc gattgtgatc ccggcacgta tcaccgaggc gctggttgaa
     8641 cgctttgccc gttctacgct gcaaatcttg ctggtgaatc acatcaatca tgccaatgag
     8701 gtagatgaaa cgttccgtca ggcgatggct aaattgcgcc gtgtcggtgt caccctgctt
     8761 aaccagagcg ttctgttacg tggtgtgaac gataacgcac aaacgttggc aaacctgagt
     8821 aatgcgttgt tcgatgccgg cgtgatgccg tattacctgc atgtgctaga taaagtacag
     8881 ggcgcggcgc attttatggt gagtgatgac gaagcacggc agattatgcg tgagttgctg
     8941 acattggtgt cgggatatct ggtgccgaaa ctggcgcgag aaattggcgg cgaacccagc
     9001 aaaacgccgc tggatctcca gctacgccag cagtaactga ttaaacaggg aagttaaaca
     9061 ttagcctgat gcgctacgct tatcaggcct acattgtgca ttgaaattga atggatttgc
     9121 aatgaactgt aggccggata aggcgtttac gccgcatccg gcaagagcaa caaacgcaag
     9181 tacagcaacc tgtcattaaa ctgggaaatt taatctttgc ccgttgcata acctttaatt
     9241 attcatatac attgctgttg ttactcttta tatagccaac accaataatt aacctttctt
     9301 ttctgtttat atagagtgag tacggctatt tatgttattt taacaactat tactaccaag
     9361 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
     9421 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
     9481 tcattgcact ggcgctgaaa attaaagaac ctcgcaatag gtaatgactc caacttattg
     9541 atagtgtttt atgtt
//
LOCUS       DAETQN010000435         6085 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.guided.199, whole genome shotgun sequence.
ACCESSION   DAETQN010000435 DAETQN010000000
VERSION     DAETQN010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.guided.199"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            561..800
                     /locus_tag="K0W68_004847"
     CDS             561..800
                     /locus_tag="K0W68_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HBI3076111.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(988..1965)
                     /gene="intI2"
                     /locus_tag="K0W68_004848"
                     /pseudo
     CDS             complement(988..1965)
                     /gene="intI2"
                     /locus_tag="K0W68_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2297..2770
                     /gene="dfrA"
                     /locus_tag="K0W68_004849"
     CDS             2297..2770
                     /gene="dfrA"
                     /locus_tag="K0W68_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA"
                     /protein_id="HBI3076112.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2865..3389
                     /gene="sat2"
                     /locus_tag="K0W68_004850"
     CDS             2865..3389
                     /gene="sat2"
                     /locus_tag="K0W68_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HBI3076113.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3458..3955
                     /locus_tag="K0W68_004851"
     CDS             3458..3955
                     /locus_tag="K0W68_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3076114.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            4016..4387
                     /locus_tag="K0W68_004852"
     CDS             4016..4387
                     /locus_tag="K0W68_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3076115.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            4797..5174
                     /locus_tag="K0W68_004853"
     CDS             4797..5174
                     /locus_tag="K0W68_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3076116.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            5197..5349
                     /locus_tag="K0W68_004854"
                     /pseudo
     CDS             5197..5349
                     /locus_tag="K0W68_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5405..>6085)
                     /locus_tag="K0W68_004855"
     CDS             complement(5405..>6085)
                     /locus_tag="K0W68_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HBI3076117.1"
                     /translation="IEAEPTWISFSRPSRIEKSRRARKSSQTILEKEEATTSENSNLV
                     STDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAFDELLSILKTKFACRVLFEETLV
                     LPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLEVDASDGVKMLSTKVLSGVDSET
                     WRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGVNHPKGLGELQVSREDMEGWAER
                     VVREQFTH"
BASE COUNT         1699 a         1392 c         1349 g         1645 t
ORIGIN      
        1 ggatcggtca actgatcctt aactgatcgg cattacaccg gtcggcagcc ctgtgggcgg
       61 acaaaatagt tgggaactgg gaggggtgga aatggagttt ttaaggatta tttagggaag
      121 agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg
      181 acaaaatagt ttggaactag atttcactta tctggttaac taatctagct aacctgccgc
      241 tcagcttagt acgatcctat atccaactga tcataatcct ttcatcagta tcccaaatta
      301 aatccccctc ctagactgcc tcaccaacac tttacagtga tcccgtattt ccggacaggt
      361 gattgatttt tttcgcttgt acaggtgtta agccacagtt atcttgatgt ggacattatg
      421 cattgtaata gcccattaac atgcgctaag agcataaatt aatatacagt aacaagcatt
      481 acgaggtgct aatgtttgga aagcagcctc ttccaactac aataataagt taactatcct
      541 ccacccacaa ataattacaa atgacccctt tccgctataa ctccgatctc accagcggct
      601 cgctacaaac ccgagagtgt cgcattatta ctggcttgtt actccaagag cttgatgaag
      661 ctgcctggga taaagccatg tataaggaaa atgtgcttca gaaacgcacg caatctacgg
      721 taagacgtat ttcttcagcg cttagaaaac gcctagaaca cctaagctct gacttttggg
      781 cttttgcgtt tttatgttag gcattgtctg gcaactggag gaagtgtaat gaccatgaat
      841 ggtcactgtg attgacagta tcttgcttaa acttatctga acaatggtag aactggctca
      901 tatttatccc ctctatgatt gtttatcttt ttcaccataa aaagtatcag gggctactca
      961 gggctacata aagatattta tctactttta ctgattgata agtagcatca gtccatccgc
     1021 aggactggtg gtgccggcaa aatgctgacc caacacatgc gtatagattt gcgtggtctt
     1081 aacatcgtta tgccctaaga gttcttgcac agtgcgaata tcacgccccg cttgtaatag
     1141 atgcgtagca aacgagtgac gaaatgtatg acaagtgaca cgcttgctaa cgatgcctgc
     1201 tttttgtacg gctgccttca atgcctttcg cgcaacggag tcatgcagat gatggcggca
     1261 taatttgccg ttatacgggt ggttgcagag cgtgctggag ggaaagacaa acatccacgc
     1321 cgcttgtcga taagcagaag ggtatttgtg atctaaagca aaaggcagcg atggccctac
     1381 gccttgtaag ttgtcgtctt gctgaataag ccgcgcttgc tcaatgagtt attttattgc
     1441 tgggattagg cgcgtgggca gtaggctgtt tctgcttttc ccacccttac cgtcatgcac
     1501 agtgatgcag ccattatcaa aatcaaaatc tttaacccgc aaacgcaagc attcattaat
     1561 gcgcaaacct gcaccataca gcagcgtaaa aataacttgg ttgcgagtat ccataacctg
     1621 caaaatgcgt tgcacttcat ttgcagagat aacagagggt agccgtctag gcttgcttgc
     1681 agggatataa tcaatatcgc ccaacggctg ttgtaaaaac ctgttgtaca aaaaagctag
     1741 ggcatttaaa gcgattttct gcgtgtttat ggctacatgt ctgctgtttg ctaagctgga
     1801 taaaaacagc ctgacctctt cactgcccat ggtctgagga tgacgttttt tgtgaaacag
     1861 aataaaacgc ttaatccagt gcaggtaagt tttttcagtt ttcagcgcat aacctttttg
     1921 tcgcatatcc gtgcgtatag aatttaaaaa tggactgtta gacataaaac gctccttgtc
     1981 ttgcaactgt ctgcctatac agcctattct agctgggatt taaaaaagtg cctgtttttt
     2041 acgcctagag atgcttgttt accggtagag ttttaattta atgctaaata aattaaaatg
     2101 ttatgagttc tttgggtgag ataatgtgca tcgtgcaagc aggatagacg gcatgcacga
     2161 tttgtaataa cagagtgtct tgtattttta aagaaagtct atttaataca agtgattata
     2221 ttaattaacg gtaagcatca gcgggtgaca aaacgagcat gcttactaat aaaatgttaa
     2281 cctctgagga agaattgtga aactatcact aatggtagct atatcgaaga atggagttat
     2341 cgggaatggc cctgatattc catggagtgc caaaggtgaa cagctcctgt ttaaagctat
     2401 tacctataac caatggctgt tggttggacg caagactttt gaatcaatgg gagcattacc
     2461 caaccgaaag tatgcggtcg taacacgttc aagttttaca tctgacaatg agaacgtatt
     2521 gatctttcca tcaattaaag atgctttaac caacctaaag aaaataacgg atcatgtcat
     2581 tgtttcaggt ggtggggaga tatacaaaag cctgatcgat caagtagata cactacatat
     2641 atctagaata gacatcgagc cggaaggtga tgtttacttt cctgaaatcc ccagcaattt
     2701 taggccagtt tttacccaag acttcgcctc taacataaat tatagttacc aaatctggca
     2761 aaagggttaa caagtggcag caacggattc gcaaacctgt cacgcctttt gtaccaaaag
     2821 ccgcgccagg tttgcgatcc gctgtgccag gcgttaggcg tcatatgaag atttcggtga
     2881 tccctgagca ggtggcggaa acattggatg ctgagaacca tttcattgtt cgtgaagtgt
     2941 tcgatgtgca cctatccgac caaggctttg aactatctac cagaagtgtg agcccctacc
     3001 ggaaggatta catctcggat gatgactctg atgaagactc tgcttgctat ggcgcattca
     3061 tcgaccaaga gcttgtcggg aagattgaac tcaactcaac atggaacgat ctagcctcta
     3121 tcgaacacat tgttgtgtcg cacacgcacc gaggcaaagg agtcgcgcac agtctcatcg
     3181 aatttgcgaa aaagtgggca ctaagcagac agctccttgg catacgatta gagacacaaa
     3241 cgaacaatgt acctgcctgc aatttgtacg caaaatgtgg ctttactctc ggcggcattg
     3301 acctgttcac gtataaaact agacctcaag tctcgaacga aacagcgatg tactggtact
     3361 ggttctcggg agcacaggat gacgcctaac aattcattca agccgacacc gcttcgcggc
     3421 gcggcttaat tcaggagtta gagagctggg gaagactatg cgcgatctgt tgaaggtggt
     3481 tctaagcctc gtacttgcga tggcatcggg gcaggcactt gctgacctgc caattgtttt
     3541 agtggatgaa gctcgtcttc cctatgacta ctccccatcc aactacgaca tttctccaag
     3601 caactacgac aactccataa gcaattacga caatagtcca tcaaattacg acaactctga
     3661 gagcaactac gataatagtt catccaatta cgacaatagt cgcaacggaa atcgtaggct
     3721 tatatatagc gcaaatgggt ctcgcacttt cgccggctac tacgtcattg ccaacaatgg
     3781 gacaacgaac ttcttttcca catctggcaa aaggatgttc tacaccccaa aaggggggcg
     3841 cggcgtctat ggcggcaaag atgggagctt ctgcggggca ttggtcgtca taaatggcca
     3901 attttcgctt gccctgacag ataacggcct gaagatcatg tatctaagca actagcctgc
     3961 tctctaataa aatgttaggc ctcaacatct agtcgcaagc tgaggggaac cactagtgtc
     4021 atacgaacct ccaagagacg gttacacaaa cgggtacatt gttgatgtca tgtatgacaa
     4081 tcgcccaagt aagtatccag ctgtgttcag aacgtacgtc cgaattccag actatgtcga
     4141 actagataac gcggaggagc tggccgacgc aatcattcga gaggttgagt taaaggaggc
     4201 atggctcaca agggtcttgg ggaactcagc cgatcctgac gcttctgtgg gcaagtggga
     4261 ccggcgccta gcgcagtgcg gtggtattga tgagatgtca agtggggtgc aggcaacata
     4321 cttcagtcca aacggtcagc tggtcggctt agtggcatac attgatgccg aatggatgga
     4381 tgaatagctc tgacgttacg attcaggtga gacaaccaga tgggcattgc gtacaagctc
     4441 agttgccacc gatgtggcta cgagtctgat cttctttacc taggccaagg tatggcgatg
     4501 cttcctgagc aaatagtcgg aacgtgcaca tgctgcgaca atctaacgac gatttctata
     4561 aatgaaagct acggagtatg ctcactttgt ggggctaagg atcgtgtgat atttacaagc
     4621 tatcagcatg aggcaaggcc aagaaatccc tgctatctag agagaagttt caaatatcaa
     4681 tgcccaagat gccatgcctt cagtatggag cctccggatc taccggagat gctttgggac
     4741 taatgctccg ctttgcctcg ggtatccgtt cgacctatgt ttaagaggaa acgcacgtgt
     4801 ctgccgccaa agctcttgcc tatgcacttt gtgacaagtt ggaaagcaaa aaagttgatg
     4861 aagtcgtacg aaatcttgtt tctagcggct ggaatattag ggaagtggcc tgggaagctt
     4921 cgtcctttga tcatgaaatg ccctactttg acacggggcg ggacttcgat attggatttc
     4981 ctgacacgct gtcatccaag attgagtttt tgaccgcgaa tggatcaatc gtccaagcac
     5041 gtgctcgcct gcacttcaag caggctctaa tttttgccaa ggcgtccaaa catgtcgccg
     5101 cgctcaagaa ctcattggat tactactatg gatcgtctgt aatcccccct caaaaccacg
     5161 gaggctattt gtagaaaatt ccattaatat gaactcaagg tgttccctac ggacgaatca
     5221 gcgatgaagg tggtgtacct ggctatccag gcggcgacca agaaatggac gatgccgatc
     5281 cgcaactgga agccggccat gaaccgtttt atgatcgaat ttggtgaccg tttaaatggc
     5341 cacctttaac tgaccggcaa aagcacttac acaaaagggt ttacaggctc tttcagtcat
     5401 tcctttaatg cgtaaattgc tctctaacca ctctctcagc ccacccttcc atatcctctc
     5461 tcgatacttg cagctccccc aacccctttg gatgattcac ccctctatgc ccgtcttgtc
     5521 catataattg atcaaacaaa ctatttggcc aattcaatga gctcttcacc actccacgcc
     5581 gtatcttttc aaaatcattc cgccatgttt cgctatcaac gccactcaac actttggtag
     5641 aaagcatttt caccccatcc gatgcatcca cctctagcaa tacaaattca ctgccattac
     5701 gttgcacccc aacggcttta atcactcttg gtgagccatc tttacacaga tgtaatcggc
     5761 tacgcccaac ttttggcaaa accaaggttt cttcaaaaag cacccgacat gcaaatttag
     5821 tttttagaat tgaaagtagc tcatcaaaag cagcaaatcg attagcaaaa atagagttgt
     5881 aattggttgc atcctgcttc ccacccacat ctgccgctgc taggacacca cctaagtgtg
     5941 gctcatcagt actaaccaaa ttactatttt cacttgttgt tgcttcttct ttttctaaaa
     6001 tagtttggct actttttctt gccctgcgag atttttcaat tcgactaggt ctactaaaac
     6061 ttatccatgt cggctcagcc tctat
//
LOCUS       DAETQN010000436         4009 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.guided.242, whole genome shotgun sequence.
ACCESSION   DAETQN010000436 DAETQN010000000
VERSION     DAETQN010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.guided.242"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..652
                     /locus_tag="K0W68_004856"
                     /pseudo
     CDS             1..652
                     /locus_tag="K0W68_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /locus_tag="K0W68_004857"
     CDS             649..1263
                     /locus_tag="K0W68_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HBI3076118.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="K0W68_004858"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="K0W68_004858"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBI3076119.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="K0W68_004859"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="K0W68_004859"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBI3076120.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..>4009
                     /locus_tag="K0W68_004860"
     CDS             3588..>4009
                     /locus_tag="K0W68_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI3076121.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHL"
BASE COUNT          971 a         1004 c         1060 g          974 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaa
//
LOCUS       DAETQN010000437         3440 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.guided.260, whole genome shotgun sequence.
ACCESSION   DAETQN010000437 DAETQN010000000
VERSION     DAETQN010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.guided.260"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..476)
                     /gene="tetR(A)"
                     /locus_tag="K0W68_004861"
     CDS             complement(<1..476)
                     /gene="tetR(A)"
                     /locus_tag="K0W68_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HBI3076122.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERG"
     gene            582..1781
                     /gene="tet(A)"
                     /locus_tag="K0W68_004862"
     CDS             582..1781
                     /gene="tet(A)"
                     /locus_tag="K0W68_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HBI3076123.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            complement(1748..1936)
                     /locus_tag="K0W68_004863"
                     /pseudo
     CDS             complement(1748..1936)
                     /locus_tag="K0W68_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(1980..2369)
                     /locus_tag="K0W68_004864"
     CDS             complement(1980..2369)
                     /locus_tag="K0W68_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3076124.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            2587..3027
                     /locus_tag="K0W68_004865"
     CDS             2587..3027
                     /locus_tag="K0W68_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HBI3076125.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT          738 a          974 c          951 g          777 t
ORIGIN      
        1 ccgcgctcgc cggcatcgct gtcgccggcc tgctcctcaa gcacggcccc aacagtgaag
       61 tagctgattg tcatcagcgc attgacggcg tccccggccg aaaaacccgc ctcgcagagg
      121 aagcgaagct gcgcgtcggc cgtttccatc tgcggtgcgc ccggtcgcgt gccggcatgg
      181 atgcgcgcgc catcgcggta ggcgagcagc gcctgcctga agctgcgggc attcccgatc
      241 agaaatgagc gccagtcgtc gtcggctctc ggcaccgaat gcgtatgatt ctccgccagc
      301 atggcttcgg ccagtgcgtc gagcagcgcc cgcttgttcc tgaagtgcca gtaaagcgcc
      361 ggctgctgaa cccccaaccg ttccgccagt ttgcgtgtcg tcagaccgtc tacgccgacc
      421 tcgttcaaca ggtccagggc ggcacggatc actgtattcg gctgcaactt tgtcatgctt
      481 gacactttat cactgataaa cataatatgt ccaccaactt atcagtgata aagaatccgc
      541 gcgttcaatc ggaccagcgg aggctggtcc ggaggccaga cgtgaaaccc aacagacccc
      601 tgatcgtaat tctgagcact gtcgcgctcg acgctgtcgg catcggcctg attatgccgg
      661 tgctgccggg cctcctgcgc gatctggttc actcgaacga cgtcaccgcc cactatggca
      721 ttctgctggc gctgtatgcg ttgatgcaat ttgcctgcgc acctgtgctg ggcgcgctgt
      781 cggatcgttt cgggcggcgg ccggtcttgc tcgtctcgct ggccggcgct gctgtcgact
      841 acgccatcat ggcgacggcg cctttccttt gggttctcta tatcgggcgg atcgtggccg
      901 gcatcaccgg ggcgactggg gcggtagccg gcgcttatat tgccgatatc actgatggcg
      961 atgagcgcgc gcggcacttc ggcttcatga gcgcctgttt cgggttcggg atggtcgcgg
     1021 gacctgtgct cggtgggctg atgggcggtt tctcccccca cgctccgttc ttcgccgcgg
     1081 cagccttgaa cggcctcaat ttcctgacgg gctgtttcct tttgccggag tcgcacaaag
     1141 gcgaacgccg gccgttacgc cgggaggctc tcaacccgct cgcttcgttc cggtgggccc
     1201 ggggcatgac cgtcgtcgcc gccctgatgg cggtcttctt catcatgcaa cttgtcggac
     1261 aggtgccggc cgcgctttgg gtcattttcg gcgaggatcg ctttcactgg gacgcgacca
     1321 cgatcggcat ttcgcttgcc gcatttggca ttctgcattc actcgcccag gcaatgatca
     1381 ccggccctgt agccgcccgg ctcggcgaaa ggcgggcact catgctcgga atgattgccg
     1441 acggcacagg ctacatcctg cttgccttcg cgacacgggg atggatggcg ttcccgatca
     1501 tggtcctgct tgcttcgggt ggcatcggaa tgccggcgct gcaagcaatg ttgtccaggc
     1561 aggtggatga ggaacgtcag gggcagctgc aaggctcact ggcggcgctc accagcctga
     1621 cctcgatcgt cggacccctc ctcttcacgg cgatctatgc ggcttctata acaacgtgga
     1681 acgggtgggc atggattgca ggcgctgccc tctacttgct ctgcctgccg gcgctgcgtc
     1741 gcgggctttg gagaaattct tcaaattccc gttgcacata gcccggcaat tcctttccct
     1801 gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga tagagaaggg
     1861 tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg gccgtcctgg
     1921 ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca gtctatcgct
     1981 tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca ttgccagcca
     2041 gtgcccgtca ctcccactct tacattgttc gcagtatcta ggtacatgtt attgcgtgca
     2101 ccttcatgat gtataatgtt attaatgaca gcagaaagat cgacagaatt aatattcttc
     2161 gtacctggga cagttatcgg gtgtcggcca gtgatgagac caaaggatgc agtataagat
     2221 gaaccactac tattatcatg gccaccatta ccaccaccgt taaaactacc tcccgaactt
     2281 ccagagccgc tatgaccatc attgtagggg tcatttggtc ccatgtgacc aaaaccaccg
     2341 acagtaccgc tatttaactc tgtactcata aaattatcct ctttgacttt taaaaacagc
     2401 aaaaactgta tttataacaa tgtgtttata tacagtattc gaaatactac gcccgtcaaa
     2461 accacaccgt caacaggttt tttgtgtatt tttcacgcct cacgggcagc ctcagacctt
     2521 ttgtgttaaa acagtaatat gttctgctgt tacaatacgg tacacacagg agacactgtc
     2581 acttttatgg catggcacac agttcaagag acactgaaat taacaggaaa gtcgcgctcg
     2641 caattatatc gtgacatggc taaaggcctt gtcagctatc gcacaggaaa aaatgatcgc
     2701 cgggagttcg agaccagtga actgattcgg gcctatggcg agctgcgcac ttctgagaca
     2761 ccaaaaaggc acaaggaggg acactccaaa tctgtcgtta gccaggcgga aaatgctcag
     2821 ctagacgtca ttcagcagaa actggacgct ctgcagcaga ctgttaccct aatgcttgaa
     2881 gacaagatgg cgcgggaagc agaaaaccgc ctgcgaaatg aggagaggga acaactacag
     2941 gccgaaatta gccgtctggc tgacgcgctg gcacaggaaa aaaagcgggg attctggtcg
     3001 cggctgtttc gctccggttc agagtaacag tgcaggtgac tctgatttac cataacatct
     3061 gttatcagca ccgcctgaga ggccctcagg gcgtttctga cgggaaaccg gtaaaacagg
     3121 caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg gcatgcatga
     3181 gtatgcgcac aagtgcatag cgcggaaatt cacaccattc agccggggta agtcctgttt
     3241 ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg tggccgtgaa
     3301 gcgttgccgg cgcagccgga aaacccgctg tcagcacgtt caggaagggg gtgatgtaca
     3361 aatggcacag aggatgaaag tgacgtatga gctgaaggaa tgatgtaaaa gtgatgtata
     3421 agtgatgtaa aagtgatgta
//
LOCUS       DAETQN010000438         2921 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.guided.276, whole genome shotgun sequence.
ACCESSION   DAETQN010000438 DAETQN010000000
VERSION     DAETQN010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.guided.276"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            996..1811
                     /gene="sul2"
                     /locus_tag="K0W68_004866"
     CDS             996..1811
                     /gene="sul2"
                     /locus_tag="K0W68_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBI3076126.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1872..2675
                     /locus_tag="K0W68_004867"
                     /note="aph(3'')-Ib"
     CDS             1872..2675
                     /locus_tag="K0W68_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HBI3076127.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2675..>2921
                     /locus_tag="K0W68_004868"
     CDS             2675..>2921
                     /locus_tag="K0W68_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-kinase"
                     /protein_id="HBI3076128.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEY"
BASE COUNT          627 a          802 c          794 g          698 t
ORIGIN      
        1 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg
       61 cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag
      121 ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa
      181 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc
      241 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
      301 cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc
      361 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg
      421 gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca
      481 ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc
      541 ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa
      601 gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt
      661 acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact
      721 atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta
      781 acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc
      841 tacttattga gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg
      901 gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag
      961 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
     1021 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
     1081 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
     1141 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
     1201 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
     1261 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
     1321 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
     1381 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
     1441 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
     1501 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
     1561 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
     1621 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
     1681 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
     1741 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
     1801 gaattcgtta actgcacatt cgggatattt ctctatattc gcgcttcatc agaaaactga
     1861 aggaacctcc attgaatcga actaatattt tttttggtga atcgcattct gactggttgc
     1921 ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg catgccttcg
     1981 cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac cgcctcattt
     2041 ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag gaacaggagg
     2101 gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg tctggagcgg
     2161 atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac agcctatcgg
     2221 ttgatcaatg tcagtttgag cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg
     2281 tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt acgccgcagc
     2341 tcgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg
     2401 atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac cctaaaactc
     2461 ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc tatgccgatt
     2521 tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa gcagagcgcg
     2581 ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct
     2641 tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg tttttcctgc
     2701 tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc tcgtttggaa
     2761 agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat
     2821 tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg gagcagtccg
     2881 gttgctcggt cgtgagaaca atctgatgtt gctcgaatat g
//
LOCUS       DAETQN010000439         2546 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.guided.291, whole genome shotgun sequence.
ACCESSION   DAETQN010000439 DAETQN010000000
VERSION     DAETQN010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.guided.291"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..93
                     /locus_tag="K0W68_004869"
     CDS             <1..93
                     /locus_tag="K0W68_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBI3076129.1"
                     /translation="GADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            154..957
                     /locus_tag="K0W68_004870"
                     /note="aph(3'')-Ib"
     CDS             154..957
                     /locus_tag="K0W68_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HBI3076130.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            957..1793
                     /locus_tag="K0W68_004871"
     CDS             957..1793
                     /locus_tag="K0W68_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBI3076131.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            2099..2341
                     /locus_tag="K0W68_004872"
     CDS             2099..2341
                     /locus_tag="K0W68_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HBI3076132.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(2373..>2546)
                     /gene="tetR(A)"
                     /locus_tag="K0W68_004873"
     CDS             complement(2373..>2546)
                     /gene="tetR(A)"
                     /locus_tag="K0W68_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HBI3076133.1"
                     /translation="GTVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRL
                     VVRNVEGPRKGDD"
BASE COUNT          522 a          721 c          760 g          543 t
ORIGIN      
        1 ggagctgact tcatccgcac acacgagccg cgccccttgc gcgacgggct ggcggtattg
       61 gcggcgctga aagaaaccgc aagaattcgt taactgcaca ttcgggatat ttctctatat
      121 tcgcgcttca tcagaaaact gaaggaacct ccattgaatc gaactaatat tttttttggt
      181 gaatcgcatt ctgactggtt gcctgtcaga ggcggagaat ctggtgattt tgtttttcga
      241 cgtggtgacg ggcatgcctt cgcgaaaatc gcacctgctt cccgccgcgg tgagctcgct
      301 ggagagcgtg accgcctcat ttggctcaaa ggtcgaggtg tggcttgccc cgaggtcatc
      361 aactggcagg aggaacagga gggtgcatgc ttggtgataa cggcaattcc gggagtaccg
      421 gcggctgatc tgtctggagc ggatttgctc aaagcgtggc cgtcaatggg gcagcaactt
      481 ggcgctgttc acagcctatc ggttgatcaa tgtcagtttg agcgcaggct gtcgcgaatg
      541 ttcggacgcg ccgttgatgt ggtgtcccgc aatgccgtca atcccgactt cttaccggac
      601 gaggacaaga gtacgccgca gctcgatctt ttggctcgtg tcgaacgaga gctaccggtg
      661 cggctcgacc aagagcgcac cgatatggtt gtttgccatg gtgatccctg catgccgaac
      721 ttcatggtgg accctaaaac tcttcaatgc acgggtctga tcgaccttgg gcggctcgga
      781 acagcagatc gctatgccga tttggcactc atgattgcta acgccgaaga gaactgggca
      841 gcgccagatg aagcagagcg cgccttcgct gtcctattca atgtattggg gatcgaagcc
      901 cccgaccgcg aacgccttgc cttctatctg cgattggacc ctctgacttg gggttgatgt
      961 tcatgccgcc tgtttttcct gctcattggc acgtttcgca acctgttctc attgcggaca
     1021 ccttttccag cctcgtttgg aaagtttcat tgccagacgg gactcctgca atcgtcaagg
     1081 gattgaaacc tatagaagac attgctgatg aactgcgcgg ggccgactat ctggtatggc
     1141 gcaatgggag gggagcagtc cggttgctcg gtcgtgagaa caatctgatg ttgctcgaat
     1201 atgccgggga gcgaatgctc tctcacatcg ttgccgagca cggcgactac caggcgaccg
     1261 aaattgcagc ggaactaatg gcgaagctgt atgccgcatc tgaggaaccc ctgccttctg
     1321 cccttctccc gatccgggat cgctttgcag ctttgtttca gcgggcgcgc gatgatcaaa
     1381 acgcaggttg tcaaactgac tacgtccacg cggcgattat agccgatcaa atgatgagca
     1441 atgcctcgga actgcgtggg ctacatggcg atctgcatca tgaaaacatc atgttctcca
     1501 gtcgcggctg gctggtgata gatcccgtcg gtctggtcgg tgaagtgggc tttggcgccg
     1561 ccaatatgtt ctacgatccg gctgacagag acgacctttg tctcgatcct agacgcattg
     1621 cacagatggc ggacgcattc tctcgtgcgc tggacgtcga tccgcgtcgc ctgctcgacc
     1681 aggcgtacgc ttatgggtgc ctttccgcag cttggaacgc ggatggagaa gaggagcaac
     1741 gcgatctagc tatcgcggcc gcgatcaagc aggtgcgaca gacgtcatac tagatatcaa
     1801 gcgacttctc ctatcccctg ggaacacatc aatctcaccg gagaatatcg ctggccaaag
     1861 ccttagcgta ggattccgcc ccttcccgca aacgacccct ggaagccccg tagcgacgcg
     1921 gagaggggcg agacaagcca agggcgcagg ctcgatgcgc agcacgacat agccggttct
     1981 cgcaaggacg agaatttccc tgcggtgccc ctcaagtgtc aatgaaagtt tccaacgcga
     2041 gccattcgcg agagccttga gtccacgcta gatctatctc atctgcgcaa ggcagaacgt
     2101 gaagacggcc gccctggacc tcgcccgcga gcgccaggcg cacgaggccg gcgcgcggac
     2161 ccgcgccacg gcccacgagc ggacgccgca gcaggagcgc cagaaggccg ccagagaggc
     2221 cgagcgcggc cgtgaggctt ggacgctagg gcagggcatg aaaaagcccg tagcgggctg
     2281 ctacgggcgt ctgacgcggt ggaaaggggg aggggatgtt gtctacatgg ctctgctgta
     2341 gtgagtgggt tgcgctccgg cagcggtcct gatcaatcgt caccctttct cggtccttca
     2401 acgttcctga caacgagcct ccttttcgcc aatccatcga caatcaccgc gagtccctgc
     2461 tcgaacgctg cgtccggacc ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc
     2521 gagagcggag cctgttcaac ggtgcc
//
LOCUS       DAETQN010000440         1813 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.guided.314, whole genome shotgun sequence.
ACCESSION   DAETQN010000440 DAETQN010000000
VERSION     DAETQN010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.guided.314"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            112..207
                     /locus_tag="K0W68_004874"
     CDS             112..207
                     /locus_tag="K0W68_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBI3076134.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            333..1070
                     /gene="erm(B)"
                     /locus_tag="K0W68_004875"
     CDS             333..1070
                     /gene="erm(B)"
                     /locus_tag="K0W68_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBI3076135.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1015..1185
                     /locus_tag="K0W68_004876"
     CDS             1015..1185
                     /locus_tag="K0W68_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HBI3076136.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFCKFGKLHVTKGNVDKLLGIL
                     LTASKELKRSLD"
BASE COUNT          663 a          303 c          295 g          552 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc aaattttgta taataggaat
       61 tgaagttaaa ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta
      121 ttccaaatgc gttatcaaat gcgttatgta gataaaacat ctactgtttt gaaacagact
      181 aaaaaaagtg attacgcaga taaataaata cgttagatta attcctacca gtgactaatc
      241 ttatgacttt ttaaacagat aactaaaatt acaaacaaat cgtttaactt ctgtatttgt
      301 ttatagatgt aatcacttca ggagtaaatt acatgaacaa aaatataaaa tattctcaaa
      361 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
      421 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
      481 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa
      541 aattaaaact gaatactcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
      601 ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta
      661 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat
      721 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
      781 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca agagtaaaca
      841 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
      901 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
      961 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccgtt acttatgagc
     1021 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
     1081 cgcttttgta aatttggaaa gttacacgtt actaaaggga atgtagataa attattaggt
     1141 atactactga cagcttccaa ggagctaaag aggtccctag actagcaaga agtacacaag
     1201 aagccttaaa gattatagaa aagcttttcc atgaaatgct taacattatt taatgttaac
     1261 atgtggcttt gtggtaacta ccacgagtgg taacttgtaa catgaaaaaa gttacaagtt
     1321 acaagtggtt tatttacgaa cataaaggct aggaaagcac ttgataagta tatcaagtgc
     1381 tttttttccg ttcgatttta ctatccaaga taccgcttga cgtacctaat attttagtgt
     1441 gctaagggct ttgctttttc tggttatagt tcgttcaaaa aagaccgcaa gggtaaatgc
     1501 acgatcatag atcgcccttg cagcttttta atctctccac ttttttataa ccaagcaaac
     1561 caccaagcac actgaaaaaa taattaggaa ctaaagcgaa gtgcgtatct tggataaatc
     1621 ttcactcgat tttaaaagat taagcaccca gccaaagcgc ctaaaaaaat aattttttta
     1681 aagaaaggaa gtaatttaaa tgactacaat tttaagcgac tacaataaat ctatcctaat
     1741 tgaattacta aacaccaatc gccaaattat cgtcgttggt gatgctgcca acttactgat
     1801 ttagtgtatg atg
//
LOCUS       DAETQN010000441         1734 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella sonnei isolate SH179
            SAMN15686839-rid12761763.guided.315, whole genome shotgun sequence.
ACCESSION   DAETQN010000441 DAETQN010000000
VERSION     DAETQN010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686839
            Sequence Read Archive: SRR12358863
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097961.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097961.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,778
            Genes (coding)                    :: 4,442
            CDSs (with protein)               :: 4,442
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 336
            CDSs (without protein)            :: 336
            Pseudo Genes (ambiguous residues) :: 0 of 336
            Pseudo Genes (frameshifted)       :: 150 of 336
            Pseudo Genes (incomplete)         :: 127 of 336
            Pseudo Genes (internal stop)      :: 108 of 336
            Pseudo Genes (multiple problems)  :: 48 of 336
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686839-rid12761763.guided.315"
                     /isolate="SH179"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..474
                     /locus_tag="K0W68_004877"
     CDS             <1..474
                     /locus_tag="K0W68_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3076137.1"
                     /translation="DDKFITTDYLQQCPTDREGLDLLRMKVEEGDVILVKKLDRLGRD
                     TADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGR
                     QEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
     gene            657..1517
                     /locus_tag="K0W68_004878"
     CDS             657..1517
                     /locus_tag="K0W68_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HBI3076138.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1667..1734)
                     /locus_tag="K0W68_004879"
                     /pseudo
     CDS             complement(1667..1734)
                     /locus_tag="K0W68_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          462 a          386 c          443 g          443 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc caacagaccg ggaagggctg
       61 gatttgctga ggatgaaggt ggaggaaggt gatgtcattc tggttaagaa gctcgaccgt
      121 cttggccgcg acactgccga tatgatccaa ctgataaagg aatttgacgc tcagggcgtg
      181 gcagtccggt tcattgatga cgggatcagt accgacggtg atatggggca aatggtggtc
      241 accatcctgt cggctgtggc acaggctgaa cgccggagga tcctagaacg cacgaatgag
      301 ggccgacagg aagcaaagct gaaaggaatc aaatttggcc gcaggcgtac cgtggacagg
      361 aacgtcgtgc tgacgcttca tcagaagggc actggtgcaa cggaaattgc tcatcagctc
      421 agtattgccc gctccacggt ttataaaatt cttgaagacg aaagggcctc gtgatacgcc
      481 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      601 cgctcatgag acaataaccc tggtaaatgc ttcaataata ttgaaaaagg aagagtatga
      661 gtattcaaca ttttcgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggtgcggta ttatcccgtg
      901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1021 gtgctgccat aaccatgagt gataacactg ctgccaactt acttctgaca acgatcggag
     1081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1201 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1261 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1321 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1381 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1441 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1501 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1561 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1621 aaatccctta acgtgagttt tcgttccact gagcgtcaga cccctgagag cagagatagc
     1681 gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcagtagct gaac
//
